LOCUS       DAOQRM010000001        60484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_196_length_60484_cov_14.806302, whole genome shotgun sequence.
ACCESSION   DAOQRM010000001 DAOQRM010000000
VERSION     DAOQRM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 60484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60484
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196_length_60484_cov_14.806302"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2083)
                     /locus_tag="PKK81_00005"
     CDS             complement(<1..2083)
                     /locus_tag="PKK81_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV31275.1"
                     /translation="MYLKSLEMLGFKSFAEAKIQFPKGITAIVGPNGSGKSNVVDSIL
                     WVLGEQSTKTLRSEKMEDVIFNGTEVRKPLGLVEVSLVIGGLGELRLDAISGLPSELS
                     EYQELMITRRLYRNGDSEYLINKTPCRLKDIRNVLIETRAGSKGHTVIEQGRIEQILQ
                     ASPQDRRELIEETAGIVRYKKQKAEALRKLEATQQNLVRVRDIVAEVKKQLNSLERQA
                     RQARSYQTLQQEARGLEIELLSRDYRTMHADLESVDHEAREVEAQEAEQVAEQARLDS
                     EQEAIRLRMNDAAEAISRVRDTLAGTEQRQSQALTAAEVERNRTELFERQRMQAAQEM
                     ERLAADQEQAQSEIETLRAMLLQLEGDIVEQEVQFQQADVEAKSLAGHRASAVAEEER
                     ARKDVLNLAVLVANTEQSLTQVTARQQETAARAERVSREQEQLVAQVAGLDQQRDLLA
                     AQREEASGRIQELMAERQAAIERIEGLGGQITGLDRDIVKFSEDVAGVESRLGALQGV
                     VREEMGYGREGEEEGTALKTCDGVREALAEWLVIPPGLDRAVETILGERVRGWLVDEP
                     SAACRAVEFLKGKELGRGAFLPQQLRWAPEQAALDSSASWWPTLNGQAGVVGRATDLI
                     RAEGASAATLSYLFDGIVFVESLDVALQLWQQHQWAAPAGPTYVTLSGETLDAAGVMT
                     GGGSSASGGLLQ"
     gene            complement(2273..3235)
                     /gene="ispH"
                     /locus_tag="PKK81_00010"
                     /gene_synonym="lytB"
     CDS             complement(2273..3235)
                     /gene="ispH"
                     /locus_tag="PKK81_00010"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNV31276.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIYVRHEIVHSRH
                     VVNSLRHKGAVFVEELNEVPEGSVVIFSAHGVAKSVWEEAQSRRLHVIDATCPLVIKV
                     HNEVNRDYTQGYELILIGHAGHPEVIGTLGQIPDKFHLVSSVKDVESLHVEKTQNLSY
                     VTQTTLSVDECRDIVEALHRRFPNIKGPHQEDICYATQNRQNAVKSLSKLVDVILVIG
                     SPNSSNSNRLRELGEHCGIPSYLIDAASDINPDWLKDAKSVGLSAGASAPEVLVTEVV
                     AYLKRLGSSEEVEELTVIEEDVEFLLPKELVTIESASRASASAN"
     gene            3409..4125
                     /locus_tag="PKK81_00015"
     CDS             3409..4125
                     /locus_tag="PKK81_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV31277.1"
                     /translation="MPPPATTHKKILIVEDEKDILQLVKLYLEKEGFRTVSAMTGTEG
                     LRQVKAEHPDLIILDLMLPELDGLEVCKRIRLNQETALLPILMLTAKAEESDTVIGLE
                     LGADDYVTKPFSPKALVARVKALLRRLERNANIPQTQYHYGPLTVDLSRHEVRLDGQE
                     VALTAKEFGLLEHLLRNIGRVLTRDVLLSAVWGYDYYGTTRTVDVHVRRLKQKLPLLD
                     DAIISIKSLGYKLRESDVPA"
     gene            4122..5981
                     /locus_tag="PKK81_00020"
     CDS             4122..5981
                     /locus_tag="PKK81_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV31278.1"
                     /translation="MNLGIRWKVALGTLAAVFVGLAVAGWLVIRSVEQTELSRMAETL
                     ETSSGLAAMTLRPLFDQSGSTVPGPLLHATVRDLSQQAHLRITVIRQDGTVVADSAVP
                     TEGLTHVENHLSRPEVAQALRSGRGMDIRASQTTGERTYYVARLLSASTPAQPGISVI
                     RLGLPLTSIDERVRHIQQDLATAFGAAFLLAMTLSLWVSRNLTKPLSEMAAAARQLAG
                     GTPGVRLVVSSTDEVGLLAQTLNQMTDQLETKIKEVSDDRAQLLAMLIAMVEGVMVLD
                     YRGTVVQVNPALERMFSLELTESRGRHYAELIRHEGLTALVSSVLQTRSGQGGEITLS
                     PSGRCLRVEASIAGGSREQEACAVFVFHDITELRRLEKIRKDFVANVSHELRTPLTSI
                     KGYVEALLDGGKDEPATATAFLEIIMRQSNRLNLILDDLLQLSQIESGQVLFRREPVD
                     LRALLERTVAVIKPLADKKHHTIELSLPDEYWVVEGDEERLVQVFINLLENAVKYTPD
                     QGRISMAIRNATHLRTTPPRAMIEIVVADSGIGIPEADRPRVFERFYRVDKARSRELG
                     GTGLGLAIVKHIVEAHSGQVWVEGNTPRGSRFVVHLPVAQESSTAVSTETGSR"
     gene            complement(5947..6939)
                     /locus_tag="PKK81_00025"
     CDS             complement(5947..6939)
                     /locus_tag="PKK81_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNV31279.1"
                     /translation="MAELSGLLLVVVILALLFDFSNGWHDSANAIATVVSTRVVSPST
                     AVLVAGVLNVAGAFMSTAVAKMVGTGIVDPQSVTQAVVASALAGAIVWNFVTLLLGLP
                     TSSSHALIGGLVGAALTHGGTAAVKFSGLRSVLEAMVLSPFFGFAIGLVLMVILSWVF
                     FRVPRALALRLFSRMQLVSASFMAFSHGANDAQKAMGIITLALVSAGVLPTAEVPTWV
                     IGSCALAMGLGTAVGGWRIVRTLGMRIVKLEPVHGFAAETGAAIVLLATAHIGLPVST
                     THTITSTVMGVGAVKRLSAVRWGVTRRILSAWLFTLPGAALLSTICYLFLSKLQ"
     gene            complement(7004..7621)
                     /locus_tag="PKK81_00030"
     CDS             complement(7004..7621)
                     /locus_tag="PKK81_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNV31280.1"
                     /translation="MFGLIPREEAFFDLFKNAAHNMIEGSRLLKDMTEDFRNPVEKAK
                     RIKDVEHVGDGITHEIARKLNQTFITPIDREDIHDLASALDDILDVVEAIADRFVLYK
                     VTAPTESAIRLANVLYQAAVEVGATVDLLGKSHQAVTECSVRVNSLENEADRISRDAI
                     SLLFEKETDPIAVIKWKEIYENFEAGTDRCEDVANILERIALKHT"
     gene            complement(7646..8638)
                     /locus_tag="PKK81_00035"
     CDS             complement(7646..8638)
                     /locus_tag="PKK81_00035"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNV31281.1"
                     /translation="MKRSRIVGTGSYVPSRVVDNQEVAAPLGLDPDQIVALTGIRTRY
                     WVEQGQASSDLAVVAGQCACEAAGITPQSVDAILVSTTSPDSAFPSTACHVQRALGAN
                     PVMAFDLSASCSGFLYGVSMADVMIRSGQVRSCLVIAAEVKSRSLDARDRETAMLFGD
                     GAGAVLVVQEDSAQPDAPGILGVRLYADGSGHGLITIPAGGSRKPVGVDTVRASEHML
                     RMQGRAVFRSAVRRLEQAMIDVLKEFGLAAGDVTRVIAHQANARILEQLRRRLGLSQE
                     AVYSVIEQYGNTSSASLPIALDQAVRTQRLAAGDLVLLGAFGGGLTWATGLVRW"
     gene            8844..9764
                     /gene="bamD"
                     /locus_tag="PKK81_00040"
     CDS             8844..9764
                     /gene="bamD"
                     /locus_tag="PKK81_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNV31282.1"
                     /translation="MHRHSRGLCVGLTIVVGMLWFAAGCSSKPKTTADAKPVSGTDEQ
                     IFLGDTIEKNYDPNVIMKRGEAFFDKEEFAEAIVEYQHFLELHRAHQLAVYAQLRLAE
                     SHLRMAKSIDRDPEPIQKAIAAFEKLRKDFPGSKYEAQALQRIADCHDWLAQTHLFVG
                     QFYYRRASYLAAAHRFDQIMKDYPDKKVAPEALYYLALTYQELGADDWAMEKLQLLAE
                     KYPNSENAGDGRRLLAKLEKKSPPAPTVVAKTDTPAPQNQPSTTLLAASVPSSSFTPS
                     ATTPSIPNLKNSPALSLGQPFVSCRLGAWC"
     gene            complement(9943..11151)
                     /locus_tag="PKK81_00045"
     CDS             complement(9943..11151)
                     /locus_tag="PKK81_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HNV31283.1"
                     /translation="MADRQLATTYDVIIVGMGPAGAVAAAALCRGGLTVLGFDKDVHP
                     RYKVCGGGLSARIDLLLEPGFKSVVEQTVNGVQFVYRGQDPLLIESSQPIAYMVMRDR
                     FDHYLVQQAIEAGTEFRTGEGVVEVTQDSDGVEVVTQEGRYRAKMVIGADGANSLVAR
                     QLFPNRSLYRAPALESEVRLGNSRHYPSATTILVDVGAARQGYGWIFPKKNGVSVGVG
                     EFRRKSSGLRTTFDRFVSEARGLSGWTVPRPVGHPIPAYSDAEGGHGDGRGSRFVNGR
                     AALVGDAGHLVDPLFGEGIYYAVRSGQLAARSILGNVHDPRESLAAYEAALERDILPD
                     FRVTARIARVIYAFPRLGFRLLRRYQDVVQSYFEVLQGRTTAVQFLPDAKKRLKASVN
                     DLLLEALHLR"
     gene            complement(11271..12017)
                     /locus_tag="PKK81_00050"
     CDS             complement(11271..12017)
                     /locus_tag="PKK81_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNV31284.1"
                     /translation="MTDSVQSISLVAAFSAGLLSFVSPCVLPLVPSYISYITGLSIEQ
                     LTDVSERNRFRKAIILNSLLFIAGFSTVFVAFGASASLLGQALITYQEHLRRFGGIVV
                     IIFGLYLLGILNLNFLKMEHRYQFRNRPAGFLGSFLIGIAFAAGWTPCVGPVLGTILL
                     YASTTESMLSGVVLLTFYSLGLALPLFLTALGVDRFLSYFKEVRAYLWGVSTVSGVLL
                     IVVGVMIYANSLTMITSFLERYGIGWYLGQ"
     gene            complement(12020..12541)
                     /locus_tag="PKK81_00055"
     CDS             complement(12020..12541)
                     /locus_tag="PKK81_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV31285.1"
                     /translation="MMLCGGFSADARGALPSSPAVPMLVVERIPQVDEEAPNFLLRDS
                     EGFNVTLDQFRGKVVLLNFWATWCGPCKIEMPAMERLYRSFSRKDFEILAVSTDAQGA
                     AVTRPFQREVGFTFPVLHDADFRIGLQYGARQLPMTFLIDRKGIIRNRIPGARDWSAP
                     AGKRLVESLLQGS"
     gene            complement(12589..13212)
                     /locus_tag="PKK81_00060"
     CDS             complement(12589..13212)
                     /locus_tag="PKK81_00060"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV31286.1"
                     /translation="MTDPINQSPANTAPRTGPSRTLVVIAAAAILGVMFLVVWLQSSK
                     YEPLVVGKEAPDFQLPDLNEKQLRLSDYRGKVVFLNFWATWCKPCREEMPSMEVLYKN
                     FEKDGLVILAVSIDRVTTKKEIPPFVKGLNLTFPVLVDSWGQTDKRYKLMGVPETYII
                     DQQGVLREKVIGPRDWTRLDNLKVLTQLLKPAEKAAHAPPAQALPTS"
     gene            complement(13216..14130)
                     /gene="ccsB"
                     /locus_tag="PKK81_00065"
     CDS             complement(13216..14130)
                     /gene="ccsB"
                     /locus_tag="PKK81_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNV31287.1"
                     /translation="MMRSLFLFDMTFWLYLAALGLYVAYLFAKRPAMQMVPAGHPIED
                     LEERDGAWATQLGQVATLVTVFGWVLNTLALTTRAFERMEHSGTFAPWSNQFEAMAYV
                     SWAIILGYVLLEFRYRIKAVGAFVVGIGFIAMGAASLLPYRYQTAEPLVPALNSYWIY
                     IHVSVTLTSYAAFAMAGGLGLMYLFKERAVNRGSQSRFYAAFPDLETIDELGYKAIML
                     GFPLLAFGIILGAMWANYAWGGYWSWDPKETWSLIVWLIYGAYIHARMTRGWEGHKAA
                     IYAIFGFLMVIFCFWGVNFLLSGLHAYA"
     gene            complement(14208..15644)
                     /locus_tag="PKK81_00070"
     CDS             complement(14208..15644)
                     /locus_tag="PKK81_00070"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNV31288.1"
                     /translation="MNDKPDAASDVSASAPRTSGLGWEEFSREVVDFFASIKLAMFLF
                     IVLAMTATIGTVIQQGERPETYVQEYGEEAYRWFLRLGFTDVYHTWWFTSLLGLLCVN
                     SLTCFHKRFPSVWRSMRQDKVSVSLAFIQGMKQQTTLSLNQSRDAVAERLVKLFAEKG
                     YRVLAKSDPGEVTVYATKGIMGRVGAHVAHLSATVIVLGGLLGSYYGFQEFGVCLEGQ
                     TYHIPRGNFDLRVDKFWIDYHENGSVKSYNSTLTVIDQGTPTTTKTITVNDPLVYKGI
                     WFYQSSYGDAWDQIEAARINIKEKGSDKIIATVDLEWNKEKAVDGLPLKMKMTDFVAD
                     FAFNSTEKKVFSKTAEHANPAIRLAVDERSSVQSTPWVFYHYPDLFEIKDSAYQFEFI
                     GYQPKKFTGLQIARNPGINMVWIGCTMLVVGMTLSSLIYHRRLWAKIIPGESGVTLHL
                     GGTTHKSQIDFQKEFRKLTEKINAQAQS"
     gene            16043..17191
                     /gene="metK"
                     /locus_tag="PKK81_00075"
     CDS             16043..17191
                     /gene="metK"
                     /locus_tag="PKK81_00075"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNV31289.1"
                     /translation="MRHNYLFTSESVTEGHPDKIADQISDGILDAIIAQDKFSRVACE
                     TILTTGIAFVAGEISTKAYVEIPDIIRDVIKDVGYTDASWGFDSNTCSVLTSIHQQSG
                     DIAMGVDSGGAGDQGLMFGYATNETTELMPMPIVLAHRLTKRLAEVRKKKILKWVRPD
                     GKSQVTVEYKDGKPCRVDTIVVSTQHSPDVTNKQIEKDLMEHVIRPSMPKGLYDPTSV
                     KHHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPSKVDRSASYMA
                     RYIAKNIVAAGLASKCEVQLAYAIGVADPVSVLVDTKDTEKVAPESLDKLVRKHFPLT
                     PRGIIDHLKLRRPIFRKTAAYGHFGRNEPEFTWEKTDKAKALRKDAGL"
     gene            17299..18555
                     /gene="ahcY"
                     /locus_tag="PKK81_00080"
     CDS             17299..18555
                     /gene="ahcY"
                     /locus_tag="PKK81_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNV31290.1"
                     /translation="MDYDVKDMGLADQGKLKIEWAEATMPVLRLIRKRFEREQPFKGI
                     RATACLHVTTETANLMKTLKAGGADVRLCASNPLSTQDDVAAALVRHEGIPTFAVKGE
                     DNKTYYRHIESAIAHKPHLSMDDGADVVSHLHSKRKDLLRNVIGGTEETTTGVIRLRS
                     MAEKKVLKFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLVCGSTLVVAGYGWC
                     GRGIATRARGMGANVIVTEIDPLKGLEAVMDGFRVMPMDEAAPLGDFFVTVTGNLKVI
                     RGEHFAAMKDGAIVCNSGHFNVELDIPALEKLSKKKLAVRSGVDQYTLKNGRRVSLLG
                     EGRLVNLATAEGHPSSVMDMSFANQALGAEYIVKNYKKLEKKVYPVPADIDKEISRLK
                     LAGMGVSIDKLTKEQVKYLASWEMGT"
     gene            complement(18880..20514)
                     /locus_tag="PKK81_00085"
     CDS             complement(18880..20514)
                     /locus_tag="PKK81_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV31291.1"
                     /translation="MLVFLIHVRDPQFYALPAKTRAKNGRIRVMGFPPIGIMSLSSVL
                     KRSGHECVMFDQAHPETPNEFIIEEIKRRKPALVGLSFLSTTSYPYAKILARQIRAAD
                     STVKLAFGGVFASLNAGLVKLQCPEIDFVCRGDGEQLLLDLLDRLEDPQEVGGVTWAK
                     DGKVINNPNRTMERHLDQWPFPDRESLELDFVESMPLDVPAVLSMERFTTMQTSRGCP
                     WPCVFCDIPIFNEGKWRARSAQHVVDELKYLEANGYGSVYFVDDHFLLQPKRIDAICK
                     GIIDAKLTIQWGIEGRVDSVAQHLFPAMAQAHCRTVMFGIESGSQKILDRLQKEQTLA
                     EVETAVRNAKKAGIEIVHGFFTVGNPDETVEDMQKTFDFASKLPLDTFGFNRLCVYRG
                     TPLWQEYIKRGLVSDAKDWYKYFKCSEIDPTCLPGAVINSVRQEGLKKLFIYKLTRYP
                     IQTFKLLRRFLRFMPFRDVAYLIIKPFLGQKKGATKAEVLSRAVEHAEMKDAAAQLTQ
                     LSDELLHSVLEESKAERQRIQQEAEGSRELPMVNSR"
     gene            complement(20816..21688)
                     /gene="sucD"
                     /locus_tag="PKK81_00090"
     CDS             complement(20816..21688)
                     /gene="sucD"
                     /locus_tag="PKK81_00090"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNV31292.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTKMVAGVTPGKA
                     GQEVEGIPVFNTVADAVKKTDCDTSLIFVPPPFCADAILEAADAGVKLIICITEGIPV
                     NDMVKVKRALHGRDVRLIGPNCPGVITVDEAKIGIMPGFIHKRGVVGVVSRSGTLTYE
                     AVHQLSTLGLGETTCVGIGGDPVNGTGFVDVLPLFEKDSETQAVVMIGEIGGDAEEKA
                     AEYIKKHVKKPVISFIAGITAPPGRRMGHAGAIISGGKGTATEKMKALEAAGVRVVKN
                     PAEIGHAVKAALGR"
     gene            complement(21700..22878)
                     /gene="sucC"
                     /locus_tag="PKK81_00095"
     CDS             complement(21700..22878)
                     /gene="sucC"
                     /locus_tag="PKK81_00095"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNV31293.1"
                     /translation="MNVHEFQAKQLFAQFGVPVPRGKEITSPEAATAWASELNTPVFV
                     VKAQIHAGGRGKAGGVKITKEKGAVAGIAKELIGKTLITHQTGPKGRKVHRLLLEEGA
                     NIAKELYLSILVDRDTGWPTFIASTEGGMEIEEVAEKTPEKIIKEAVDPAVGFQGHNG
                     RNVAFALGLQTMEPAVINPFVQMLGNLYRLFMEKHAALVEINPLIITKEKTLVALDGK
                     VSFDDNGIFKHDDVQNMRDLNEEDPLEIEATANNLNYVKLDGNIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKDTVAAGFRILLKDPNVKGIFINIFGGIVRCERIAHG
                     VIEAAKEVKITVPLVVRLQGTNADEGRKLLAESGLKLEVANDLWEAAQKIVKLTGKAA
                     "
     gene            complement(22964..24565)
                     /locus_tag="PKK81_00100"
     CDS             complement(22964..24565)
                     /locus_tag="PKK81_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNV31294.1"
                     /translation="MDIHALQQIVHQTRDARRKQTIPKFSPADRDQLIHKYHPDYRTS
                     AYRPIRFGPNANDKTVVELAALLEGDSSIAENIDLTPHYTTDVLVVGGGGAGCAAALH
                     AHGTGAKVILATKLRLGDSNSVMAQGGMQISVAPEDSPVTHFLDTLKGGHMQNDHELL
                     KVMVEDGPSIAKWLIELGVLFDRQADGNLHVKKGGGSSKPRLLTCSDYTGLEIMRVLK
                     DEVLNQKIQLLEFCAAVELLSDDHGHCTGAIFKDLDNHRQVVVAAKSVILATGGIGRL
                     HIQGFPTSNHYGATGDGLCLSYRMGAKLDHIDTFQYHPSGAVYPEQLIGALVTEGIRS
                     EGGHLVNAKGERFVNELDTRDVVSSSIIRECEEGRGIRTMSGRVGVWLDTPLLDAEHG
                     PGTVEKHFPAMMMQFERFGIDISKDPVLIYPTLHYQNGGVKIDANSETNVKNLFVAGE
                     ASGGLHGRNRLMGNSLLDLMVFGKRSGLTAASRAGSMPQGKLTLQHLRRFRAEAKQHG
                     SSSGVISPMILPAYTRKEPERTANG"
     gene            complement(24635..25345)
                     /locus_tag="PKK81_00105"
     CDS             complement(24635..25345)
                     /locus_tag="PKK81_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNV31295.1"
                     /translation="MAQEDTNVIDQPEVLRHKMVTIEISGKKYEVPEGITVIKALWHT
                     GQEVVRGAGCLGGFCGACATYYRTKDDPKVRTCLACQMAVEDGMSFTIMPPFPARKAL
                     YDIQSLKDPKQDLFNLYPEAPLCRNCNACTEACPQKIDVREGVWKAVFGDFKSVSEMF
                     MDCVMCGMCTPVCIADIAPNLVALYVSRAQGAHFTEKPQGLDIRIKEIQDGRFNDEWN
                     KVLAMNEQELADHCATVK"
     gene            complement(25430..27661)
                     /locus_tag="PKK81_00110"
     CDS             complement(25430..27661)
                     /locus_tag="PKK81_00110"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNV31296.1"
                     /translation="MTTKADKIIYTKTDEAPMFATYSLLPIINAFSKAAGVSVELRDI
                     SLAGRILAVFPEHLTPEQRQPDALSELGELAKKPEANIIKLPNISASLPQLQAAIKEL
                     QSQGYKLPEYPENPKDDKEKDIKSRYDKVKGSAVNPVLREGNSDRRAPLSVKAHTRKH
                     PHKMGAWSSDSKSHVSHMKGGDFFSNEKSLTTTAATEVRIEFVGQDGKTTVLKPKVAL
                     QAGEVVDATFMSVKALRAFLEEQIQDSAKQGVLFSLHMKATMMKVSDPKIFGHAVTVY
                     YKDVFAKHGETLKKLGVDPDNGLGDLYAKIKALPDDQRKAIEADIQAVYKQRPPMAMV
                     NSDKGITNLHVPSDIIIDASMPPVIRDSGKMWGPDGKLADTKCVIPDASYAPVYREVI
                     DFCKKHGALDPKTMGSVPNVGLMAQAAEEYGSHDKTFKVPGNGTIRVVDASGKALLEH
                     KVEEGDIWRMCQVKDAPIRDWVKLAVTRARATGAPAIFWLNKDRAHDAQLITKVNQYL
                     KEHDTNGLDIRIMTPADATRLSLERIKEGKDTISVTGNVLRDYLTDLFPILEIGTSAK
                     MLSIVPLLNGGGLFETGAGGSAPKHVQQFQEEGYLRWDSLGEFLALAASLEHLAKVAN
                     NPAAKMLADTLDQANAKFLESNKSPARKVGEIDNRGSHFYLALYWAQALAQQTQDKNL
                     AARFVKVAKEMADNEAKISGELIAAQGKPVDTGGYYHPDDAKSSKAMRPSTTLNAIID
                     AIA"
     gene            complement(27687..29933)
                     /locus_tag="PKK81_00115"
     CDS             complement(27687..29933)
                     /locus_tag="PKK81_00115"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNV31297.1"
                     /translation="MSMDLAKKLYAKMPEVFAKARKQFGRGLTLADKILVSHADNFDA
                     QIWERGKAMLALRPDRVAMQDATAQMAMLQFMQANKEKAAVPSTIHCDHLIRAEMGSE
                     KDLLRAMDENREVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGCLIIGTDSHT
                     PNGGGLGGLAIGVGGADAGEVMAGLPWEVLHPKLIGVRLTGKLNGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDEKMVAYMTITD
                     RADLATFAQSHRDLLVADPEVYQSPEKYYDQIVEVDLSTLEPHVVGPHTPDLARPISK
                     LAAEAKAKGYPVELKAALIGSCTNSSYEDISRSAHVAEQGLKAGLKAKASFLVSPGSE
                     RIYHTMKRDGFLDTFEKLGGTVLSNSCGPCIGQWKRADGVKGKADSIVSSFNRNFPGR
                     NDGISETLSFLASPEVVTAYAITGDLGFDPVNQMLKGADGKEFKFQPPQGEELPAKGF
                     AKGEEGFVAPAESGEGLTVDIPPTSERLQLLQPFPRWDGKDFEKLPLLIKTKGKTTTD
                     HISPAGPWLKFRGHLDKISDNMFLGANSAFTSEPGRGTNVLTGEANLTIAQIARDYKS
                     KGIGSVVVGDENYGEGSSREHAAMSPRFLNVKVVITKSFARIHETNLKKQGIVALTFA
                     DPKDYDKIEQQDRISVTGLNNLAPGKPVQVTIHKADGKALTIQTNHSITEQQIAWFKA
                     GSALNALN"
     gene            complement(30048..31868)
                     /locus_tag="PKK81_00120"
     CDS             complement(30048..31868)
                     /locus_tag="PKK81_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNV31298.1"
                     /translation="MSILANKDTRVVIQGGQAGVNAARRMAEFCYLIKRPLNVEAFVY
                     PPDAGKSNEIPYGSGLIAIPVYKSIAEATKHHPTLNTSLVYIGADRAMKGGIEALDDS
                     HIKVVSMITEGVPEKDAKILGAHARKLGKVFNGPSSIGIISAGACRLGVIGGAFDNLV
                     LSKLYREGSFGVITKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRVKLIAVVSGFCQESLPKGMKFGHAGA
                     KEGMKGEGSARSKSDALKKAGALVPPTFGALGPAIKETYQDLLKSGQIKEPVEPATLP
                     RLPKTIEEAMKADEVMVSPLIRTTISDDRGDEPCYDGYPASELINKGYEIPHVIGLLW
                     DKRLISKQEAEIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQSVAAGMIMIGPRF
                     GGAVTDAGRFFKYAVDNKMSVDEFLAYMKKNHGPVPGIGHRVKSLRNPDKRVKELVGY
                     VKSLNIKTPCLDFALEVEKITSVKKDNLILNVDGTMAAVLVDIGFPVDSLNGFFILSR
                     TIGLIGHWVDQKRQDSRLIRLFDYLVNYAAPKRREVPPLK"
     gene            complement(31911..33110)
                     /locus_tag="PKK81_00125"
     CDS             complement(31911..33110)
                     /locus_tag="PKK81_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HNV31299.1"
                     /translation="MAKVLEGPGMGLMKKWGISVPNYVVVTSVDELTRLGQANEWLKK
                     SKLVAKAHEALGSRFKLGLVKIDLDFKAAEAATKEMIGRQVGSITVTQVIVSEMIPHK
                     EEYYCAVKSTREGSEILVANCGGIEVESNWERVKRLCLEIGQAPSPESLEKLSKEAGF
                     GGPLTKKMAEFAGKMFACFDSEDAQYLEVNPVVLRAADEELVALDAVTLLDGDAKFRH
                     PDWNFAFAAEFGRAYSKQELEVMAVDSKIKGSVKFIEIPGGDTAMLPAGGGASVYYSD
                     AVVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIRNIIVGGAIANFTDVKKTFGG
                     IINGFRKAKSEGKLKSVKIWVRRGGPREKEGLDAMRALKEEGFDINVFDRNTPLTDIV
                     DKALQAK"
     gene            complement(33311..34165)
                     /gene="mutM"
                     /locus_tag="PKK81_00130"
     CDS             complement(33311..34165)
                     /gene="mutM"
                     /locus_tag="PKK81_00130"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNV31300.1"
                     /translation="MPELPEAEVAARQLRERVVGATVRDCWIGREDIVREGLPSLDQY
                     RQAKIAGVERKGKSVILHFLCGKEPRFLVAELGMTGLLLFRSTLTKHPQHTHFILHLD
                     GCCEPDIRYWNPRRFGRLSLLDRAGLDRYVARRFGHDPLTISHEQFLRVLRATRSRLK
                     SLLMRQQVIAGIGNIYANEILFRSRLHPNQPSNSIPGKSITRLHQVMGEVLREAIAMG
                     GSSVRDYFAPDGTEGQYKRRHLVYAKAGEPCPNACGAVICRSIGERSSFYCPTCQRNG
                     RRRATRPE"
     gene            complement(34354..34776)
                     /locus_tag="PKK81_00135"
     CDS             complement(34354..34776)
                     /locus_tag="PKK81_00135"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HNV31301.1"
                     /translation="MINKRLLVGNADDARNPPPQVSAVLMVAEEQNVTVPSRVIYAKI
                     PLKEFGEPTAAALYEAVEWIAAHVADNRLMVCCRVGMGRSVSVVIAYLCCVEGMAYAD
                     AVKLVLTRRPGGMPLPRLQETIQDVCRRRQTQVNSSTS"
     gene            complement(35500..>35780)
                     /locus_tag="PKK81_00140"
     CDS             complement(35500..>35780)
                     /locus_tag="PKK81_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31302.1"
                     /translation="KTLVPRDPDYHRVVSELRVMKGEKRVWLCDQTGRSEIGRQDKES
                     SMSNTAFDGWHRGAIIRVSDIVRKERKGRPAMVGRIISSGTVEIVRSA"
     assembly_gap    35781..35880
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<35881..36740)
                     /locus_tag="PKK81_00145"
     CDS             complement(<35881..36740)
                     /locus_tag="PKK81_00145"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HNV31303.1"
                     /translation="MFTRNGQVGSRVYFEDPQLCAGYLVYYLPVNLAKVQILLDELQP
                     VLPVAQDQDFRVLDLGGGPGTGVLGVLDWCLSKSARPPSTLRMIAVDRSPHVLRVSTD
                     IWRAYCEQQRDQSIPPLNTVECNLERSLPSAIRDGGGVNGYQLIIVQNLLSELFVGSM
                     DSVGQRTALIGELLNYLAPEGSLMLIEPATRPASRELHQVRDNLLAGQHCSVFAPCLH
                     DAPCPALLKPDDWCHEERKWKTPAWIAQVDREVGLLKDALKFSYVIFRKDGKTLVPRD
                     PDYHRVVSELR"
     gene            36926..37174
                     /locus_tag="PKK81_00150"
     CDS             36926..37174
                     /locus_tag="PKK81_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31304.1"
                     /translation="MTDPILQAYLEVEMAMERFTLVLHDHVDHLRKTEAPGSDKLHRM
                     ANGTKAMRDSASIYLSYAKYVAHGMPESPDLVEEDLQG"
     gene            complement(37268..38614)
                     /locus_tag="PKK81_00155"
     CDS             complement(37268..38614)
                     /locus_tag="PKK81_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNV31305.1"
                     /translation="MSEALEPKQKTNPEGDPTTSVSAPKAETKKDPHADAKRQKVVTP
                     EYMFLEAPRTKEFITGSEAAKEAIRRSNVDLAIAYPITPQSETMQLVGVLYGEGYVKE
                     YYRGEEEVGVMAAIAGGSRAGVRCYTATAGPGTLRGLEGIASWPGHRLPVVAMFTCRV
                     VNAPLAIQPDNIEVSYLLNCGMIVFHAENQQDMYDFTLAGFIISEKNDVTLPVGVCCD
                     GFFVTHARGYVRMQDRGMKLPPREPWRGAVPVLDAENPPARLSRDAPVQKSNFMAYNI
                     HAVWQQEVWAAVERSRKYINQYMGGLLTAENVDDAEAVIIASGSAAAQSREAVRICAE
                     KGIKIGLIKIRSLRPFPTQELRKLCGKAKLIVVPEFNYVGWLAKEVATAIYGFSNAKI
                     IGGPRVYGGQSMPVELIVDEVESGLTGKKSTNVAISQVMGASAADHEAMGHFMRSI"
     gene            complement(38653..39411)
                     /locus_tag="PKK81_00160"
     CDS             complement(38653..39411)
                     /locus_tag="PKK81_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HNV31306.1"
                     /translation="MTQDTRERIIVPGPAGFHPPSAAQLGVALPDPGEGLYYGLLEPN
                     EDIVIEEMARKMLTSPNATIFPGPLVLWAWNNHAVEKAKAVLEIAAQIPEVMIIPMPD
                     YRPKYPKIDPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNCCTMA
                     ICAEQGHEDAMLTIRDSDTLKLKRTAQIFKKVREEMGIKLPDNGENVRFTGTQSKVHN
                     GKTHTNPMTFMPSAAGVGSAATFGHSAEQMKREG"
     gene            complement(39789..40502)
                     /locus_tag="PKK81_00165"
     CDS             complement(39789..40502)
                     /locus_tag="PKK81_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV31307.1"
                     /translation="MAKRFNIRMAGVGGQGVVTGSHILSTAVINAGGESTIVPFYGSE
                     KRMAPVESYVRVSDEPIYEIGEITFPHIIIIFHPQVITHGKSYTMPFYFGLKEDGIAL
                     INNDGPMKLHRDQARELEERRARLYYFPATKLSLEVAGMDLCTNMALMGCIGAITGLT
                     TVEALEQSVKDRFLGKGFVVSGGTAALDSVVERKFKKKQELIDKNVAVIQAGWNYAVD
                     HGWAAPTVKRSEIRATVSA"
     gene            complement(40526..41425)
                     /locus_tag="PKK81_00170"
     CDS             complement(40526..41425)
                     /locus_tag="PKK81_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV31308.1"
                     /translation="MSLDYVKFSSGFEKFMPKEYRDMVEHGPFGKKVSVSQMGSFKEV
                     LEEHPMCAGCAMTLFIRLAMIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTNRGAVLKMAPLGKKFEKMDMLQMAKVAGCAYVATVVPNNPRRVESVI
                     KKAVLIAREVGSTYIQAYTSCNIEYAIPTDKVMEDAKNVENDRYQFSEYITDEAKQFL
                     TERYGYKEFLPKPAAPAPGLPKA"
     gene            41891..42139
                     /locus_tag="PKK81_00175"
     CDS             41891..42139
                     /locus_tag="PKK81_00175"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNV31309.1"
                     /translation="MPIRVLVPGEEHGEQHAGGVHKRPPIPDGSLKAECPKFMSHGPC
                     GGVRKGGFCEVYPEMTCPWVTLYNKLNELGQLEWMKQV"
     gene            42163..42591
                     /gene="queF"
                     /locus_tag="PKK81_00180"
     CDS             42163..42591
                     /gene="queF"
                     /locus_tag="PKK81_00180"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNV31310.1"
                     /translation="MTAKGSKKRKPARATTKLGYNERHAKSGISAPLPDIETFPNQYK
                     GYEITIVIPEYTAICPKTNLPDFGTITLHYKPDKHCLELKALKTYIHAYRNVGIFYEN
                     AVNRILQDIVRACRPIKATVTGEFAARGGLRSVIEAKYPA"
     assembly_gap    42731..42830
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42831..43970
                     /locus_tag="PKK81_00185"
     CDS             <42831..43970
                     /locus_tag="PKK81_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31311.1"
                     /translation="KKNLGDLSLDDFANYTGVRLRGNVLRTHNFKQQNPPGAESGAVQ
                     NKSELGFPEVFSLFTAGTLSKNIGVFAELESNLEEGTTGMERAFITFNNVGGENIANI
                     RIGKLDPSAVFSFSTLRQQLEFIGESTNSSTDAVQRAGLFPLATAAKFYGLRDRSGAV
                     ISPYAPSLYNAVAETGIEVRGRPFGDWFMYQVGMLNGSNEGFGDSNKGKDFYGAIRLD
                     YARSANFSASLSGFAYLGNSNATVFDGTRTVDVNWNRYGAAAHLRYKMLDFHGMYTLD
                     RIKHVPTAALSNFDTTASGVTVALDAYVTNRTLLSLRYDNMDAGGDLTQRTSQSFAGM
                     QLKHYLRSNVAVYARHDLNLRRAEEGNAAAHNLRHAVFVGIDISY"
     gene            43984..44757
                     /locus_tag="PKK81_00190"
     CDS             43984..44757
                     /locus_tag="PKK81_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV31312.1"
                     /translation="MHRHVLRYLAILIVTGFCATTAQALLPKDGRDTITEGHRIYDRA
                     CAWCHGSEGKGDGPSGWSIGRYAAPRPRDFTAESYKFRSTPSGELPTDQDLFRTITQG
                     IPGFMPSYRSLNEQERWQVIAYLKSLNPAFEQEQPTPIALPSPPPLPSGNGITNGRAL
                     YAKYECRTCHGDNGAGDGPESKAGHLRDAHNLPITATDLTDPSSFKNGATPHDLYRSI
                     MSGLDGTPMPAYADQFAGQEEATWDLVWYLQSLSGQPGR"
     gene            44833..45552
                     /locus_tag="PKK81_00195"
     CDS             44833..45552
                     /locus_tag="PKK81_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNV31313.1"
                     /translation="MKPILLTVTLCLMLGQSALLRAESPAGTLVGRITYAGPIESGQV
                     VAVTRDSSFCGATTAIHTVTVNAKTRGLQGAVVSIDGDTVPMADSPRRPLVLTNTHCA
                     FSPRIVAGEVGQQLEIRNDDPVMHNTHISMGPRTFVNVAMIPASRPVEKPLKQPGIYL
                     VKCDAHKFMRATVLAFTHPFFSVTDDTGAFQIAQLTPGEHVVRVWHDTLGTFQQRVTI
                     PTRGEATITIEYPAHVDANKK"
     gene            45620..>48165
                     /locus_tag="PKK81_00200"
     CDS             45620..>48165
                     /locus_tag="PKK81_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31314.1"
                     /translation="MAVLITIGMAGFATFRLIEIRQSIEQATQARALAVSRTFSMVGA
                     AAVLDNLFRIQEALGRYAQDSEILSILIIDPDNMIVAATNPQQIGQQLTDETLAQAHE
                     TNAETISHGQTMGGVPTLLVATPLLNEQDIATWVRIEFSLAAMQEELTREARRLFLLS
                     VLFMGATISVAQLGIRRMSGLFRETAKKLQETLHTLHCVSEQAPSGDETSDNGNSPAS
                     PSCKGELEQVVDLVETTTTLLTTQAQRLQSFTDSLEQAVRERTIELHQAKEAAEAANR
                     AKSQFLANMSHEIRTPMNGVLGMTELLLTTTLTPKQRSLADTLHRSGTGLLDIINQIL
                     DFSKIEAGKLKLEMIEFGLRQTVEDAVDLFAETAGRKHLELTCFVPPNIPDTVIGDPV
                     RLRQILLNLVGNAMKFTHTGDVAVTLELITHTTTNLTLKFRVRDTGVGIPEAAQARLF
                     QAFSQADNSTTRKFGGTGLGLAIVKQLALLMGGDVGVDSVSGQGSTFWFTVELERAAQ
                     RTAASGATERPLDGFSIFIVDDNSTNRQILETHLTAWGAAVLSATSGLEALALLDRQC
                     TARRRIHLAVLDIHMPDMDGITLARTIREDARLSGMGLVALSSVDQVTDESQRYPSLF
                     SAWLRKPVHQSLLKDCLTRMCHHVPATEAPAPTQPDDHAVPFAAHVLLAEDNPVNREV
                     AFTMLELLGCTTTMVENGREAVEALTNRPFDLVLMDCHMPEMDGLTATALIREQETQA
                     AQPRHTTIVALTANALEGDRERCLAAGMDDYLSKPFTLTTLKEVLHRRLSIKGPSRTT
                     EPLLRSTPQAPPPQTAPPTGPAAPGDDHVDLNAWQAIRARQRPGQPDLLH"
     assembly_gap    48166..48265
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            48340..>52498
                     /locus_tag="PKK81_00205"
     CDS             48340..>52498
                     /locus_tag="PKK81_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV31315.1"
                     /translation="MRRFLQRLRQSVRRTSGQLATDSGDSQTEHREIPPSNTPPSLDE
                     GRALDAVLCSLEEGLCIVDSQWHILRLNPQAEILIGAPAHGLKGRPAYQLIAPGPEEF
                     RHECLVTDRSIPPLQSGLPYRTDNGLLLRQDGQLTPISLTITPMSHDGVVRGAVLTFR
                     DLTAQKEAESHQIENSALLRRLQTGMVRLASNQAIYRGQLTEAFHLITRVTTQSLRVA
                     RVSIWFYAPDHAVLRCADLYDQPGDQHSHGIEQPAALYPQYFSSLEAGEMIVANEAQA
                     DPRTVEFAAHHLLPLGITSMLNVPIRAEGILVGALHCEHIGTPRVWTTEEQQFAASVA
                     NTVSLAVEAADRHKAETETKRAEQFLDSVIENLPIMVYVKEAEQLRFTRWNRAAEELT
                     GFSRELILGRTGHHFFRKEEADRFTRHDREALRSGRLIEVASEEIHTQHRGVRLLRTK
                     KLPVMDEQGRPRFLLGIAEDITDRTAHESSLHESEEKYRELFESSRDAIMILSAPNWN
                     FTACNPATVQLFGARDAEHFTSLGPWDVSPEHQPDGEESMVKAPKMIGIALQEGSHNF
                     EWLHKRVGGPSFLASVQLTRITLKGPPGLQATVRDITEQRRSEAALQAYSTFQKAMLD
                     NAGHAIISCKPDGMIQVFNPAAEALLGYSADELVGKQTPGIFHDLDEVVARARQFSKE
                     LGSTIEPGFDVFVEKCRRNLPNEHEWAYVCKDGTRKTVLLNVTALREADGRITGYLGI
                     ASDITPLKLAAQELVSAKEAAEAANVAKSRFLANMSHEIRTPLNGVLGMTELLLSTSL
                     SAKQQSLLETVRRSGVALLEVINDILDFSKIEAGKLELEQVEFGLRQVVEEAVEIFAG
                     SAGNKKLELTCFIPADVPDSIVGDPVRLRQILLNLIGNAIKFTTQGEVAVAFALTKQH
                     ADTFTLACQVRDTGIGIPDAAQAQLFTAFTQADGSTTRRFGGTGLGLAIVKQLVQLMG
                     GEVGLVSAPGQGTTFSFTFTVTRGSAHAAYPPLAERHLAGRQILIVDDARTNLEILAT
                     HLRSWGATVFTAESADDALLQLEHFRHTQHRIDMAILDLRMPGRDGVDLARSIKQQTA
                     YQNLPLIALSSVERLSDDSDVTHRLFHSFLRKPVRQSLLKGCLTQVLGGSAGTVTPSP
                     QSSAAPRPRVEAHILLAEDNPVNQDVASSMLEMLGCRITIAPNGRAAVEAATTGRFDL
                     ILMDCQMPEMDGFTATAAIRRQEATASDRRHVPVIALTANAMEGDRARCLAAGMDDYL
                     TKPFTVAQLHAVLTQWLTPQPAKASETDQASSPHTTDADEPAATEEAPVSTTIDKTAW
                     DAILSLQRPGRPDILARVLATYLDDSRLLVEQIRSAVQSQDAVVLCQAAHRLKSSSAQ
                     LGVLATAA"
     assembly_gap    52499..52598
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <52599..52790
                     /locus_tag="PKK81_00210"
     CDS             <52599..52790
                     /locus_tag="PKK81_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31316.1"
                     /translation="AHRLKSSSAQLGVLATAAHCKELETLGRLARIDEAAHLLSQLIE
                     AHQFACAAITSELQQRSAR"
     gene            52857..53675
                     /locus_tag="PKK81_00215"
     CDS             52857..53675
                     /locus_tag="PKK81_00215"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNV31317.1"
                     /translation="MATYAIGDVQGCFASLRQLTKTVGFNPAQDRLWFVGDLVNRGPD
                     SLAVLRYIRGLGPAAVTVLGNHDLFLLAVATGLATLRRDDTLTQVLDAPDCDELTTWL
                     RQQPLLYREGSYVLVHAGLLPHWTIEEAQQLAMEAETALRGEQFSATLRALHPSGHLQ
                     WAPDLKGPVRLATIIKVLTRIRTCSDDGIMESTFSGPPKLTPQGFHPWFDLPNRRSAT
                     ATIVFGHWAAMGLHLTPNLCALDSGCVYGRQLTAVRLEDRKVFQVRCEETRGGK"
     gene            53662..54201
                     /locus_tag="PKK81_00220"
     CDS             53662..54201
                     /locus_tag="PKK81_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNV31318.1"
                     /translation="MEVNEPPLTVDWSRYSSRALPTYRFLPGLTPHPRRDPLGHSFAE
                     PEPRPRPFAQHQWPDSEDYLYAVDLYNFGYWWESHEVLEGLWHACGRRTTAGNYFQAL
                     IQFAAAHLKRALGNEVAAHNLAHSGLLRLQNSPASYMGLDTVTFAHDITSWMGDHSRP
                     AVRIRLESFGNGPSVNEAP"
     gene            complement(54357..55145)
                     /gene="panB"
                     /locus_tag="PKK81_00225"
     CDS             complement(54357..55145)
                     /gene="panB"
                     /locus_tag="PKK81_00225"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNV31319.1"
                     /translation="MTVPDLQKCKRDRRKIAVVTAYDALFARMVEQAGIDVILVGDSL
                     GVVVQGKSNTLSVTMEEMLYHTKLVAGAAQRSLVIGDMPFLSYQVSQEEALRNAGRFI
                     QAGAHAVKLEGGAAVADRVSAIVKIGVPVMGHLGMTPQSVHQYGGYKVQGKGKDRAQQ
                     LLEDAQALEAAGAVGIVLEAIPAGLAKTVTEALTIPTIGIGAGPHCDGQVLVLYDLLG
                     LFDEFVPKFVKPYAHLKVDALQALRRYKDEVEQGTFPSDSESYH"
     gene            complement(55145..56035)
                     /locus_tag="PKK81_00230"
     CDS             complement(55145..56035)
                     /locus_tag="PKK81_00230"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNV31320.1"
                     /translation="MSREPRRLKDVLAQGQFAVTVEYNPPKGTNLTHVVESAKALVGR
                     VHGVNVTDNTAAIVRAGSLPVCRVLYELGHDPVMQLTCRDRNRIAMQSDLMGAHILGI
                     RNILCLTGDYPTVGDHKEAKPVYDLDSVQVMQLVTGLNNGKDYAGNKLDGSTAFTIGG
                     AVTPEADPLGPMLVKFEVKVRAGAEFFQTQAIYQPEQFKKFMEAVRPFKVKVLAGILL
                     LRSAKMAEFMNANIPGVCVPQDMIDEMRAAGDKRALDVGVEIAVRTIKAVRPYCDGVH
                     IMAIKSTERLPEILTKAELG"
     gene            complement(56208..57068)
                     /gene="folD"
                     /locus_tag="PKK81_00235"
     CDS             complement(56208..57068)
                     /gene="folD"
                     /locus_tag="PKK81_00235"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNV31321.1"
                     /translation="MAARIIDGKALAQQVRERLAVESAAVLAKTGVKPGLATILVGDD
                     PASHVYVRNKQKACELAGIYVDDHKLPASTTQAELLALIEKKNADPKIHGILVQLPLP
                     KHIESRVVLEAVSPNKDADGFHPYNFGRLVEGNPVFEACTPKGVIKMIESTGVSIEGK
                     RAVVVGRSNIVGKPLALMLLQRNATVTICHSKTKDLAAVCREADLLLVAIGKAKFVTA
                     DMVREGAVVIDVGTNKLPDGKLCGDVDFEPVSQKAGWISPVPGGVGPMTIAMLLDNTV
                     ESAKRMAGMK"
     gene            57325..58356
                     /locus_tag="PKK81_00240"
     CDS             57325..58356
                     /locus_tag="PKK81_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31322.1"
                     /translation="MNSRTIRHNRFTRLLRRRSFGLALALAIGVLFHQPWAWALQVSG
                     LESPHSFLADPATRSYYISNINGEADARDNNGFITKLSDDGRITAFKFIEGGRGDVTL
                     HAPKGMVVVDDVLYVTDLDTLRAFDKTTGKPLATLVLPRPAAGGAAQSLTDVASDGQG
                     HLYLADQGGNAIYRVDLVPTLTLSLYVSGAHLAGPSGVAVHPKTGHVVVVSYDSGKIF
                     DITPEGTLTELASNGFFTARFQNLSGVDFDRWGSMYVSDGTKGKIWRMLPNGKFQVIA
                     EYLPGPSDVGIDRVNHLILVPYQDSNAAEVNGLESPTASSGDRAKRTLADYGFVEPKK
                     SDKEGSPRK"
     gene            58353..59051
                     /locus_tag="PKK81_00245"
     CDS             58353..59051
                     /locus_tag="PKK81_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31323.1"
                     /translation="MSRCVYCKQRKGKRSCPALTGLICSQCCGEHRLTRISCPPDCDY
                     LDTGSDYQQKRLGEQFAPVRRELYRQLSVAGGEKAAALFNLIEVVTFGYFHDRRDGQD
                     AEVFAAIQALRRTLSPLHVPSAPMPVFAERLKKEYDTFVKQQPQQVSDAGSAPEILDR
                     AVAILTEFSGQDFQSRRFLNGMIGYVKTFHPEIAEHLTKQHEAGRIVLPGQLTPPPQE
                     PTHVHGPECHHHHH"
     gene            59245..>60484
                     /locus_tag="PKK81_00250"
     CDS             59245..>60484
                     /locus_tag="PKK81_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNV31324.1"
                     /translation="MGSFVMDLLASLPKGDALAEPAWNSRHRGILSILWLHVLSVPMF
                     GIYMGASPSLYLGGGGLLAFIAVAARLSVVRRRLQSAIATCGLMTASALLVHLSGGQI
                     ELHFHFFVMMSVIVLYQDWLPFLVGLQFIILDHGVVGTLMPDMVYGHAQGQTHPWTWA
                     LIHGGFILAECAALLYFWRVNELAQNEVRQSEARTRMIIETALDAVITSDATGTITGW
                     NTQAELMFDVPRTEAIGKYLTTYVSASYPSTEPGPITPYAGLVPGAILNRRSEMLGHT
                     CQGTSFPVEIAMSCLTVGGTQQFTIFVQDISERKQQEERLHRAKEAAEAASLAKSQFL
                     ANMSHEIRTPMNGVLGMTELLLTTPLNDKQRRYADTVHNSGTSLLHIINDILDFSKIE
                     AGRLVLEYIPFGLRQMLTETI"
BASE COUNT        13265 a        18784 c        15748 g        12287 t
ORIGIN      
        1 gttggagcaa gcccccactg gcactgctgc cgcccccggt catcacaccg gcggcgtcca
       61 gcgtttcacc ggataacgtg acatatgttg gcccggcggg agcagcccat tgatgctgct
      121 gccaaagctg cagcgccaca tccagtgact ccacaaacac aatcccgtcg aacaaatagc
      181 tcaaggtagc ggcagaggcg ccttccgcgc ggatcaaatc cgtcgcgcgc cccaccacgc
      241 ccgcctgccc gttcagcgtc ggccaccacg aggccgatga atcaagcgcc gcctgctcag
      301 gcgcccatcg caactgctgc ggaagaaagg cgccgcgccc cagttcttta cccttcaaaa
      361 actccaccgc gcgacaagcc gccgacggct catccaccag ccagccgcga acacgctccc
      421 cgaggatcgt ctctacggcc cggtccagcc ccggcggaat cacgagccac tcggcgagtg
      481 cctcgcgcac gccgtcgcac gttttcaacg ccgtgccttc ctcctcacct tcccggccat
      541 atcccatttc ctcacgcacg acaccctgca gggcaccgag gcgcgattcc acaccggcga
      601 cgtcttcgga aaacttcacg atatcccggt ccagcccggt gatctgcccg cccagccctt
      661 cgatccgctc gatcgccgcc tgccgttcgg ccatgagttc ttgaatgcgc ccgctggcct
      721 cttcccgttg cgcggccagc aagtcgcgct gctgatccaa cccggccacc tgtgccacca
      781 actgttcctg ttcgcgcgag acccgttccg cccgggcggc ggtttcctgc tgacgggccg
      841 tcacctgcgt caggctctgc tctgtattgg ccacgaggac ggccaggttc aacacatctt
      901 tccgcgcgcg ctcttcctcc gccaccgcgg aagcgcgatg cccggccaac gatttcgcct
      961 cgacatccgc ctgctggaat tgcacttcct gttcgacgat gtccccttcc aattgaagca
     1021 gcatcgcccg aagggtctcg atttccgact gcgcctgctc ctgatccgcc gccaaccgtt
     1081 ccatctcttg cgcggcctgc atgcgttgac gttcaaacaa ttccgttcgg ttccgttcga
     1141 cctccgcggc cgtcaaggcc tgcgactgcc gctgttcggt gccggccaac gtgtcacgta
     1201 cacgcgaaat ggcctccgcc gcgtcgttca tgcgcagcct gatcgcttcc tgctcagagt
     1261 cgaggcgggc ttgttcggcc acctgttccg cttcctgcgc ttccacctcg cgcgcttcgt
     1321 gatcgacgga ctccaggtcg gcatgcatgg tgcgatagtc gcgggacagg agttcgattt
     1381 cgaggccgcg agcctcctgc tgcaacgtct gataggagcg ggcctggcgg gcctgacgct
     1441 ccagcgaatt cagctgtttt ttgacctcgg ccacaatgtc ccgtacccgc accagattct
     1501 gctgcgtcgc ctcaagtttg cggagggctt cggccttttg ttttttgtag cggacgatac
     1561 cggccgtttc ctcgatcagc tcccggcgat cctgcggcga ggcctgcagg atctgctcaa
     1621 tccgcccctg ctcgatgacg gtatggccct tggacccggc cctggtttct atcagcacgt
     1681 ttcgaatgtc tttgagccgg cagggtgtct tattgatgag atattcgctg tctccgtttc
     1741 gatacagacg gcgggtgatc atcagctcct gatattcgct cagttcgctt ggcagcccgg
     1801 agatcgcatc cagccgtaat tctcccagcc cgccgatgac cagcgacacc tccaccagtc
     1861 ccaacggctt gcgaacctcg gtgccgttaa agatgacgtc ttccatcttc tcgcttcgaa
     1921 gcgtcttcgt actttgctca cccagtaccc agaggatgga gtccacgaca ttacttttgc
     1981 cgctcccgtt cggacccacg atggccgtga tgcctttcgg aaattggatc ttcgcctccg
     2041 cgaacgactt gaagccgagc atttccaatg acttcagata caccggcgac ctccagagca
     2101 agaaggttga gatgggacgg attgtgagga ataaaacgaa aatcttctat cacagcgata
     2161 gaatcaatgc aaagaaaaac acctgatatg ggggaataat ggcgccactc ccccaacata
     2221 tggaggagtg gcggaaacgg tatggaggaa gaacggacgg cagccgacga agctaattcg
     2281 ccgaggccga cgcgcgcgaa gccgattcga tcgtgaccaa ttctttggga aggagaaact
     2341 ccacatcttc ttcgatgacc gtcagttcct cgacctcttc cgaagagcca agacgcttca
     2401 gataggccac cacttcggtg accaggactt cgggagccga agccccggcg gacagcccca
     2461 cactcttggc atctttcagc caatcgggat tgatgtccga cgccgcatcg atgagatagg
     2521 agggaatgcc gcaatgctcg cccaattcgc gaagccgatt cgaattcgag ctgttcggtg
     2581 atccgatcac gagaatcaca tcgaccagct tcgacaacga cttgaccgca ttctggcggt
     2641 tctgggtggc ataacagata tcttcctggt gcggcccctt gatattgggg aatcgccggt
     2701 gcaacgcctc gacaatgtcc cgacattcat cgacgctcag cgtggtctgg gtcacatagg
     2761 agagattctg cgtcttctcg acatggaggc tctccacgtc tttgaccgag gacaccaggt
     2821 ggaacttatc cgggatctgg cccaacgtcc cgatcacctc cgggtgtccc gcatggccga
     2881 taaggatcaa ttcataccct tgcgtatagt cgcggttcac ttcgttatgc accttgatca
     2941 ccagcggaca ggtggcatcg atcacgtgca ggcggcggct ctgcgcctcc tcccacaccg
     3001 atttcgccac accgtgggca ctgaagatca cgaccgatcc ttccggcact tcattcagct
     3061 cttccacaaa cacggcaccc ttatgccgca gcgagttcac gacatgccgg ctgtggacga
     3121 tctcatggcg cacataaatc ggggcgccgt attttttgag cgacaaatcg acgatatcta
     3181 tggcgcggtc gacacccgcg cagaatccac gaggattggc taaataaatc ttcatctgct
     3241 gattccctct cacgctcatg aagggccgcc cggcctgccg ggaaaagcgc gtagtctcac
     3301 taaacggtca ccttacgtca gacccgccct tgtcgtcaac cggatcatct gccccgctcc
     3361 tccgcaaaag cagttgaggc cgcggaacca ttgtgggaga atcctgccat gcctcccccc
     3421 gcaacgacac ataaaaaaat tctgatcgtc gaagacgaga aggacattct ccaactcgtc
     3481 aagctctatc tggagaagga agggttccgc accgtctccg cgatgaccgg cacagagggg
     3541 ctccgccagg tcaaagccga gcatcccgac ctgatcattc tcgacctcat gttgcctgaa
     3601 ctcgacgggc tggaagtctg caaacgaatc cggctcaatc aagagacggc cctgcttccc
     3661 attctcatgc tgaccgccaa ggcggaagaa tccgacacgg tcatcggcct cgaactgggc
     3721 gccgacgact acgtgaccaa acccttcagc cccaaggcgc tggtggcgcg cgtcaaggcg
     3781 ctcctccgac ggctggaacg gaacgcaaac atcccccaga cgcaatatca ctacggcccc
     3841 ttgaccgtgg atctctcccg gcacgaggtc cggctggatg gacaggaagt cgcgctcacg
     3901 gccaaagagt tcggcctgct ggaacacctg ctgcgcaaca tcgggcgggt gctcactcgc
     3961 gacgtgctgc tcagcgccgt gtggggctac gactactacg gtaccacgcg cacggtcgat
     4021 gtccacgtgc gccggctcaa acaaaaactt ccgctgctgg acgacgccat catttcgatc
     4081 aaatcgctcg gatacaaact acgcgaatcc gacgtacctg catgaacctc ggcatccggt
     4141 ggaaagtcgc cctgggcacc ctcgccgccg tcttcgtcgg cttggccgtc gcggggtggc
     4201 tggtcatccg ctcggtggag caaactgaac tcagccgcat ggcggagacg ctggagacca
     4261 gcagcggctt agccgccatg actctccggc cgttgttcga tcagtccgga tcgaccgtac
     4321 caggccccct gctccacgcc accgttcgcg acctgagcca gcaggcccac ctccgcatca
     4381 ccgtgatccg gcaggacggc accgtcgtcg ccgacagcgc cgtccctact gaagggctga
     4441 ctcacgtcga aaaccacctc tcgcggccgg aagtcgcgca ggcactgcga tcgggacgcg
     4501 ggatggatat ccgggccagc cagaccaccg gagaacgcac gtattatgtc gcccgcctgc
     4561 tgtcagcctc gacgccggcg cagcctggca tttccgtcat cagactcggg cttccgctga
     4621 cctcgatcga tgagcgcgtg cgacacattc agcaagactt agcgacggcg ttcggcgcgg
     4681 ccttcctgct ggccatgacc ctgagcctct gggtctcgcg caatctcacg aaaccgctct
     4741 cagagatggc cgcagccgcc cgccaacttg ccggcgggac gcccggcgtc cggctggtgg
     4801 tctcctcgac agacgaagtc ggcctgctcg cgcaaaccct caaccagatg accgaccagc
     4861 tggaaaccaa aatcaaggag gtttcggacg accgcgcgca actgttagcc atgctgatcg
     4921 ccatggtcga gggcgtcatg gtgctggatt atcgcggcac cgtcgtccag gtgaatccgg
     4981 cgctggaacg gatgttctct ctggaactga cggaatcgcg gggccggcac tatgccgaac
     5041 tcatccggca cgaagggctg accgcccttg tctcctccgt gctacagacc cgatccgggc
     5101 aaggcggcga aattaccctc tcgcccagcg ggcgatgtct gcgggtcgaa gcctcgatcg
     5161 ccggaggcag ccgcgagcag gaagcctgcg ccgtatttgt gtttcacgac atcacggaac
     5221 tgcgccggct ggaaaaaatc cggaaagatt tcgtcgccaa cgtgtcgcac gaactgcgca
     5281 cgccgctgac gtccatcaag ggctatgtcg aggcgctgct ggatgggggc aaagacgagc
     5341 cggcgacggc gacggccttc ctggaaatca tcatgcgcca gagcaaccgg ctgaatctga
     5401 ttctggatga cctgctgcag ttgtcgcaaa tcgagtccgg gcaggtcctg ttccgtcgtg
     5461 agccggtgga cttacgggcg ctgctggaac gaaccgtggc ggtgatcaaa cccctggcgg
     5521 acaagaaaca tcacacgatc gagctgtcac tcccggacga atattgggtg gtcgagggcg
     5581 atgaggaacg gctggtgcag gtcttcatca acctgctcga aaacgcggtg aaatacaccc
     5641 cggatcaggg acggatctcc atggcgatcc gcaacgctac acacctccgg acgaccccgc
     5701 cccgcgcgat gatcgagatc gtcgtggccg actcaggaat cggcatccct gaagcggacc
     5761 ggccgcgtgt cttcgagcga ttctaccggg tcgataaagc ccgctcgcgc gaactcggcg
     5821 ggaccgggct gggcctcgcc atcgtcaagc atatcgtgga agcccatagc gggcaggtct
     5881 gggtggaagg caatacgccc agagggagtc gattcgtggt ccacctcccc gtcgcgcaag
     5941 aatcgtctac tgcagtttcg acagaaacag gtagcagata gtcgacagta gcgcagcccc
     6001 cggcaaggtg aagagccatg ccgacaggat tcgtctggtc acgccccaac gcaccgccga
     6061 gagccgcttg accgccccga cacccatgac ggtggaggtg atggtatggg tcgtgctgac
     6121 cggcaaaccg atatgagccg tcgccagcaa cacgatagcg gctccggttt cagccgcgaa
     6181 gccatggacc ggttccagct tcacgattcg catgccgagg gttcgcacga tccgccaccc
     6241 gccgaccgcc gtgccgagcc ccatcgccag ggcgcaggaa ccgatcaccc aggtgggcac
     6301 ttccgcagtt ggaagcactc cggcggacac caacgccaac gtgatgatgc ccatggcctt
     6361 ctgcgcatcg ttcgccccgt ggctgaatgc catgaaactg gccgagacca gctgcatgcg
     6421 tgagaacagc cgcaaggcca gggcgcgcgg cacccggaaa aatacccaac tcaggatcac
     6481 catcagcacc agcccgatgg caaacccgaa aaacggagac aacaccatcg cctcaagcac
     6541 cgagcgcagg cccgagaact tgacggccgc cgtgccgccg tgggtcaacg cagcaccgac
     6601 cagccccccg atcagcgcat gagaagagct ggtcggaagt cccagtaaca gggtcacaaa
     6661 attccacaca atcgccccgg ccaaggccga ggccaccacc gcctgcgtca ccgactgcgg
     6721 atccacgatc ccggttccga ccatcttggc gacggcagtc gacatgaacg ccccggccac
     6781 attgagcaca cccgccacca gcaccgccgt cgacggactc accacacgcg tcgacaccac
     6841 ggtcgcaatg gcattggcgc tgtcgtgcca gccgttggaa aaatcaaaga gcaatgccag
     6901 aatcacgacg accaacaaca acccgctcag ttcagccatg aatgccgatt cctacccttt
     6961 gaatagtgat caggattggt gtcgagggag acgcggagga cgctcacgtg tgctttaacg
     7021 cgatgcgctc aagaatattg gcgacatctt cgcaccgatc cgtccccgct tcgaagtttt
     7081 cgtagatctc tttccatttg atcacggcga tcgggtccgt ctccttctcg aacaacagag
     7141 agatggcatc gcgggaaatg cggtcggctt cattctccaa gctattgacc cgcacgctgc
     7201 actccgtgac ggcctggtgc gacttgccca gcaagtccac ggtcgccccg acctccaccg
     7261 ccgcctgata caacacgttc gccagccgga tcgccgactc cgtgggagcc gtcaccttgt
     7321 agaggacaaa tcggtcggcg atggcctcga ccacatccag gatatcgtcg agggcactcg
     7381 cgagatcatg aatatcctct cggtcgatgg gcgtgatgaa tgtctggttc agcttcctgg
     7441 caatctcatg ggtaataccg tccccgacat gctcaacgtc cttgatgcgt ttggcctttt
     7501 cgacaggatt ccggaaatcc tccgtcatat ctttcaggag acgactcccc tcgatcatgt
     7561 tgtgcgccgc attcttgaac aaatcgaaaa atgcttcttc ccgaggaatg agaccgaaca
     7621 taacgtcctt tctagccgcg aggggttacc agcgcaccag tcctgtcgcc caggtcaagc
     7681 ccccgccgaa tgcgcccaac aacaccagat cccctgccgc aagtcgctgt gtccgaaccg
     7741 cctgatccag cgcgatcggc agggaggccg acgatgtatt cccatactgt tcaatcaccg
     7801 agtagaccgc ctcctgcgag aggccgagac gacgacgcag ctgctccaaa atccgcgcat
     7861 tggcctgatg ggcaatgacc cttgtcacgt cgccggccgc aagcccgaac tcctttaaga
     7921 catcgatcat tgcctgctcc aggcgacgta ccgccgatcg aaacaccgcg cggccttgca
     7981 tacgcagcat gtgctcactc gcacggacgg tgtccacccc gacgggcttc cgcgaccccc
     8041 cggcgggaat cgtaatcagg ccgtggcccg agccgtcggc atagagccgc acaccaagaa
     8101 tgcccggcgc gtccggttgg gccgagtcct cctgcacaac cagcaccgct ccggctccat
     8161 cgccgaagag catggcggtc tctctgtccc tcgcatcgag agaccgtgat ttcacctcgg
     8221 cggcaatgac caggcaggag cgaacctgcc cgctccgaat catgacatcc gccatcgata
     8281 ccccatagag aaaccccgag caggaagccg acaggtcgaa tgccatgacc ggattcgcac
     8341 ccagcgcacg ttggacatgg caggccgtcg agggaaaggc gctgtcggga gaggtcgttg
     8401 aaacaagaat cgcatcgaca gactgtggag ttatcccggc ggcctcgcac gcgcactgcc
     8461 cggcgactac cgccaagtcc gacgacgcct ggccctgctc tacccaatac ctggtccgaa
     8521 tcccggtcaa agcgacaatc tggtccgggt ccaggccgag cggagcggct acctcctggt
     8581 tgtctacaac ccgggagggc acgtacgaac cggttccgac aatgcgactg cgcttcacgg
     8641 ggttcctatt cactcgcctt tggagaccgt ctgggatgac atgtcaggcg acctcacgag
     8701 gtgcggcgag attataggac ccgggcccag gagggtcaac ctccacaagc cgaattcgtt
     8761 gcttttcgct tcctgatctg ctagaaaaat tgtgtagtta cgatcagtcc acgttctcga
     8821 ctatttaccg aaggagtcgc tggatgcaca ggcattcaag ggggttgtgt gtcggtctca
     8881 ccattgtggt gggaatgtta tggtttgcag ctggatgctc cagcaaaccg aagacgaccg
     8941 ccgacgccaa acccgtaagc ggcacggatg agcagatctt cctcggcgac acgatcgaga
     9001 agaactacga tccgaacgtg atcatgaagc gcggcgaggc gttcttcgac aaggaggagt
     9061 ttgccgaggc catcgtcgaa taccagcatt tcctggaatt gcatcgagcc catcaactgg
     9121 ccgtctatgc ccagctccgt ttggcggaaa gccatctccg gatggccaag tcgatcgacc
     9181 gagacccgga gccgatccaa aaggccatcg ccgcgtttga aaaactgcgg aaggatttcc
     9241 ccggcagcaa gtatgaagcg caggcgctcc agcgtattgc cgattgccac gactggctcg
     9301 ctcagacgca cctgttcgtc ggtcagtttt actatcgtcg agcctcctat ctggcagccg
     9361 cgcatcgatt cgatcagatt atgaaagact atccggacaa gaaggtggcg ccggaggcct
     9421 tgtactattt ggccctcacc tatcaggaac tcggtgccga tgattgggcc atggaaaaac
     9481 ttcaacttct agccgagaag tatccgaaca gcgagaacgc cggcgatggc cgccggctac
     9541 tggccaagct ggagaagaaa tcgccacctg cgccgaccgt cgtggccaag accgacacgc
     9601 cggctccgca gaatcaacct tcgacgacac tgcttgcggc ctcggtgcca tcgtcgagct
     9661 ttacgccttc cgctacaaca cccagcatcc ccaacctgaa gaattcgccg gccctctccc
     9721 ttgggcaacc gttcgtttcc tgccggctcg gcgcctggtg ctgataggcg tcgaacctcc
     9781 cgttcatcca cccatgtaca ggccgttcag aacacctccc gcaagactgc agcgaagtcg
     9841 acgtcgcgac gaataaggcg ggccacgcct gccgactccg tcgggacggt gaggcgccgg
     9901 tgtcatacga gaacgccatt ggcggacgtc tccaccagcc tgttaccgta gatgtaatgc
     9961 ttcgagcagc aggtcgttga ccgacgcctt gaggcgcttc ttcgcatcgg gcaagaattg
    10021 tacggctgtg gtacgtccct gcagaacttc gaaataggac tgcaccacat cctggtagcg
    10081 tcggagaagc ctgaagccga gtcgggggaa ggcataaatg acccgggcga ttctggccgt
    10141 gactcgaaaa tccgggagaa tgtcacgctc caacgctgcc tcgtaggcgg cgagcgactc
    10201 acgggggtca tgaacgttcc ccaaaatcga ccgggctgcg agctggcctg aacgaaccgc
    10261 gtaataaatg ccttcgccaa acagcgggtc gactaagtga ccggcatcac ccaccaatgc
    10321 cgctcgacca ttgacgaatc gagagcctct tccgtctcca tgcccgcctt cggcatctga
    10381 ataggcggga atcggatggc caaccggacg aggcactgtc cagccggaca gcccccgcgc
    10441 ttcgctgaca aaccgatcga aggtcgtgcg caaaccgctc gatttgcgac gaaactctcc
    10501 tacccccaca gacacgccat tctttttggg gaaaatccat ccgtacccct gacgggcggc
    10561 ccccacatcc acaaggatgg tggtcgcgct cggatagtgt cggctattcc cgagccgcac
    10621 ctcgctttca agtgccggcg cccgatacag ggagcggttg ggaaacagct gccgcgcaac
    10681 cagactattg gcgccatccg caccgatcac catctttgcg cgataccgtc cctcctgcgt
    10741 caccacctcc acaccatcgg aatcctgagt gacctcgacc accccctcac cggtccggaa
    10801 ctcggttccg gcttcaatcg cttgttgcac aaggtagtga tcgaaacgat cgcgcatgac
    10861 catgtaggcg atcggttgag aggattcaat gagcagcgga tcctgcccac gatagacaaa
    10921 ctggacgcca ttgaccgtct gttcgacaac cgatttgaat ccgggctcta agagcaggtc
    10981 gatgcgcgcc gagagcccac cgccgcacac cttgtaccgg ggatggacgt ccttgtcgaa
    11041 accgagcacc gtcaagcccc cgcgacagag agccgcagca gcaaccgctc ctgccggccc
    11101 cattccgaca ataatcacat cgtaggttgt ggccaattgc ctatcagcca tgtctcagct
    11161 cccagttgaa ggacacagag ctccgacctg gccttggcca agggtagacc acgcactgta
    11221 acgcaggcgt caggggggag cccgatagga aagggaaaga caggccggag ttactgaccg
    11281 aggtaccagc cgatcccata gcgttcgaga aagctcgtga tcatggtcag cgaattggca
    11341 tagatcatca cacccaccac gatgagcaac accccgctca ccgtcgacac accccagaga
    11401 tatgcgcgca cttctttaaa gtaactgaga aatcgatcga cgccgagcgc ggtgagaaac
    11461 agcggtaggg ctaaccccag cgaataaaac gtgagcaata ccacaccact gagcatcgat
    11521 tctgtcgtac tggcatagag caggatcgtc cccaggacag gtcccacgca gggagtccag
    11581 cctgcagcaa acgcgatgcc gatcaggaac gaacccagaa acccggccgg gcggtttcgg
    11641 aattgatacc ggtgctccat tttcaaaaag tttaaattca agataccgag gagatagagg
    11701 ccgaagataa tcacgacgat gcctccgaac cggcgcagat gctcctggta ggtaatcaac
    11761 gcctggccca agaggctggc cgatgccccg aacgcgacaa acaccgtcga aaacccagcg
    11821 atgaacagca gggaattcaa aatgatcgct ttccggaagc gattccgttc agagacgtcc
    11881 gtgagctgtt cgatcgagag cccggtaatg taggaaatgt acgaggggac gagcggcagc
    11941 acacagggcg acacgaacga cagcagcccg gcggagaatg cggcaaccaa tgaaatcgat
    12001 tgcaccgaat cggtcatggt taggacccct gcaacagcga ttcaaccagg cgcttccccg
    12061 caggggcgct ccagtctctg gcccctggaa tccgattgcg aatgatgccc ttgcgatcga
    12121 tcagaaacgt catggggagc tggcgtgcgc catactgaag cccgatgcga aaatcggcgt
    12181 cgtggagcac ggggaaagta aagccgactt cccgctgaaa gggacgggtc accgctgcgc
    12241 cctgggcgtc ggtcgagacc gcgagaatct caaagtcctt gcggctgaag cttcgataca
    12301 gtcgctccat cgccggcatc tcgattttgc agggcccgca ccaggtcgcc cagaaattca
    12361 agagcacaac tttccctcgg aactgatcga gcgtgacgtt gaacccctcg gagtcccgaa
    12421 ggaggaagtt cggagcctcc tcatcgacct gaggaatgcg ctcgacgaca agcatcggca
    12481 cagccggcga ggaaggcaat gctccgcgag cgtcagcaga aaaccctcca cagagcatca
    12541 tgaccgcacc ggccatagat aatattgagc agcgcgtcat catcaccgtt acgacgttgg
    12601 cagcgcctga gcagggggcg catgggcggc cttctccgca ggcttgagaa gttgggtcaa
    12661 caccttaagg ttatccagtc gtgtccaatc ccgaggcccg atcacttttt cacgcaggac
    12721 accctgctga tcaatgatgt aggtttcggg gacacccatc aatttgtagc gcttgtcggt
    12781 ctggccccag gaatcaacca gtacgggaaa cgtgaggttc aagcccttca cgaacggagg
    12841 tatttctttc ttcgtcgtca cccggtcgat gctgaccgcg agaatcacca acccgtcctt
    12901 ttcaaaattc ttgtacaaaa cctccatcga aggcatttcc tcacggcacg gcttgcacca
    12961 ggtcgcccag aaattcaaaa agaccacttt gccgcgatag tccgataacc gcagctgctt
    13021 ctcgtttaag tcgggcaatt gaaaatccgg tgcctctttt cccaccacca atggttcgta
    13081 cttggaactc tgtagccaga ctacgagaaa catcacgccc agaatggccg cagcggctat
    13141 taccaccagg gtacgggacg ggccggttcg cggcgcagtg ttggcagggc tctggttgat
    13201 tggatctgtc atggtctagg cgtaggcatg cagaccggag agcagaaaat tcactcccca
    13261 gaaacagaaa atgaccatga gaaaaccaaa gatcgcgtag atcgccgctt tatggccttc
    13321 ccacccccgc gtcatgcgag catggatata ggcgccatag atcagccata cgatcaacga
    13381 ccaggtctcc ttgggatccc aactccagta cccgccccag gcatagttcg cccacattgc
    13441 tccgaggatg atcccgaagg ccagcagcgg aaaccccagc atgatcgcct tatagccaag
    13501 ctcatcgatg gtttcgagat cgggaaaggc ggcataaaac cgcgactgac tgccacgatt
    13561 gaccgcccgt tctttgaaga gatacatcag gcccaatcca ccggccatgg caaacgccgc
    13621 gtagctggtc aacgtgacgg agacatggat gtagatccag tagctgttca gggccggaac
    13681 gagcggctct gccgtctggt atcgataggg caagagcgac gcggcgccca tggcaataaa
    13741 cccgattccc accacaaagg caccgacggc cttaatccgg tagcggaatt cgagaagcac
    13801 gtagcccaag ataatggccc aggacacata ggccattgcc tcgaactggt ttgaccaggg
    13861 cgcgaaggtg cctgaatgtt ccatccgctc aaatgcccgc gtcgtgaggg ccaacgtatt
    13921 cagcacccac ccgaacacgg tgacgagggt tgccacctgc cccaattgtg tggcccatgc
    13981 gccatcacgt tcttcaaggt cctcaatcgg atgcccggct ggaaccatct gcatggcagg
    14041 acgttttgcg aacagatagg ccacatacaa ccctaacgcg gctaaataca accagaacgt
    14101 catatcgaat aggaacagtg atcgcatcat acggacctcc gtctgtctat cctagcgtgc
    14161 agcgcagacc gaatgaaacg ttgctctcgg tcgcgcggca gcgatcttca ggattgggcc
    14221 tgagcgttaa ttttttccgt caacttccta aactcttttt ggaaatcgat ctggcttttg
    14281 tgcgttgttc cgccaagatg gagcgtcaca ccggactcgc cgggaatgat cttcgcccat
    14341 aaccgccgat gataaatcag cgacgacaga gtcatgccca ccaccagcat cgtacacccg
    14401 atccagacca tattgatgcc aggatttctg gcaatctgca gcccggtgaa cttcttcggc
    14461 tgatatccga tgaattcgaa ttggtatgcg gagtccttga tctcgaagag gtcgggataa
    14521 tgatagaaca cccagggagt ggactgtacg ctactccgct catcaaccgc caaccgaatc
    14581 gcgggattgg catgctccgc ggtttttgaa aacaccttct tctcggtcga attaaacgcg
    14641 aagtcggcca caaaatccgt catcttcatc ttcaatggaa ggccgtcgac ggctttttcc
    14701 ttattccact ccagatcgac cgtagcgata atcttgtcgc tgcccttctc cttaatgttg
    14761 attcgagccg cctcgatctg atcccacgca tccccatagc tggattggta aaaccagatc
    14821 cctttgtaga ccaagggatc gttcaccgta atcgtcttgg ttgtggtggg agtgccctga
    14881 tcgatcacgg tcaaggtgct gttgtaggat tttaccgagc cgttctcatg gtaatcgatc
    14941 cagaacttat cgacacgaag atcgaagttg ccacgcggga tatggtaggt ctgcccttcg
    15001 aggcataccc cgaactcctg aaacccataa tagctaccca gtagcccccc gagcacgatg
    15061 accgtggcgc tgaggtgggc cacatgcgcg ccgacccggc ccataatccc cttggtggcg
    15121 tagaccgtca cctcgccggg atcgctcttg gccagcaccc gataaccttt ttcagcgaag
    15181 agctttacaa gtcgctcagc cacagcgtct ctcgattgat tgagtgagag ggtggtctgc
    15241 tgcttcatcc cctggataaa ggccagcgac acactgacct tatcctgacg catggaccgc
    15301 catacactgg gaaaccgttt atggaaacaa gtgagagaat tgacgcacag gagccccagc
    15361 agactggtaa accaccaggt gtgatagaca tcggtgaaac ccaatcgcag gaaccagcga
    15421 taggcttctt ctccatattc ctgcacatag gtttccgggc gttcgccctg ctgaatgacc
    15481 gtgccgatcg tcgcagtcat ggcgagcaca atgaacagaa acatcgccag cttgatcgaa
    15541 gcaaaaaaat ccacgacctc gcgtgaaaac tcctcccagc caaggccgga ggtgcgcgga
    15601 gcggatgcgc tcacatcgga tgctgcatcg ggtttatcgt tcatgaatca acatacctag
    15661 ctgcttcgac ctggctgtca tacaaatccc atcatcatac ccaatgacga gctgcgtgtg
    15721 gaatcctgcg ccaatgcagg tcggtaccac gaggctggaa gtgccggggc ggagcgttta
    15781 tgatgaaaaa tgttccaaat gcgcgaaaag taagctatct tacaagtcgt cttaaaggac
    15841 tgtcaagcaa gacgcttgtc cggcgcgaga ctcaaccaat tccaccagaa cggtggacaa
    15901 aaacagaccg gtcagctact attgccttag ttttttgcgg agttgcctga tcatcttaag
    15961 cgtagcggct tccacaatca gtcgccgtac cacctgactt caatcgccgc atcctcgtat
    16021 cacttcaatt ccagggggaa caatgagaca taactacctg ttcacgtctg agtctgtcac
    16081 cgaagggcac ccggataaga ttgccgatca gatctcggac ggaattctag acgccatcat
    16141 cgctcaagac aaattttcgc gagtcgcctg cgaaaccatt ctcacgaccg gcatcgcatt
    16201 cgtcgccgga gaaatctcga ccaaggccta tgtggagatt cccgatatca ttcgcgacgt
    16261 gatcaaggac gtcggctata cggacgcctc ctggggtttc gattccaaca cctgctcggt
    16321 cttgacgtcg atccaccaac agtccggcga tatcgccatg ggagttgatt ccggcggcgc
    16381 cggcgaccag ggcctcatgt ttgggtatgc caccaatgaa acgaccgagc tcatgccgat
    16441 gccgatcgtg ctggcccatc gattgaccaa acgtttggcc gaagtgcgca agaaaaagat
    16501 tctcaaatgg gtgcggcccg acggcaaatc gcaggtgacc gtggaatata aggacggcaa
    16561 accttgccgc gtcgatacca tcgtcgtttc cacacagcac agccccgacg tgaccaacaa
    16621 gcagatcgag aaggatttga tggaacacgt gatcaggccg tccatgccga agggtctgta
    16681 cgatccgacc agcgtgaaac accatatcaa cccgaccggc cgattcgtgg tcggcggacc
    16741 gatgggcgat accggcctga ccggccgcaa gatcatcgtc gatacctacg gcgggcacgg
    16801 cagccacggc ggaggggcct tctcgggaaa agatccatcg aaggtagatc gctccgcctc
    16861 gtacatggct cgctatattg caaagaacat cgtggccgcg ggcctggctt caaaatgcga
    16921 agtccagctg gcctacgcca ttggagtagc cgatcccgtc tccgtattgg tcgacaccaa
    16981 ggacaccgag aaggtagcac ccgaaagctt ggacaagctg gtccggaagc atttcccatt
    17041 gaccccgcgc gggatcatcg accatctcaa gctacgtcgc cccatcttta ggaagacggc
    17101 cgcctacggg cacttcggtc gcaacgaacc ggagttcacc tgggaaaaaa ccgacaaagc
    17161 caaagcgttg cgcaaggacg caggcctcta accgcctaca gcgcgtcgca ccagccaagg
    17221 gggcgggtcg caagcccgcc ccttttttct gcattcacgc atcactcgat tatcagtcac
    17281 aacacaggag gacgaccagt ggactacgac gtgaaagaca tgggattagc tgatcaggga
    17341 aaattgaaaa tcgaatgggc agaagccacg atgccggtac tccggctcat tcgcaaacgc
    17401 tttgaacggg aacagccgtt caagggcatc cgcgccaccg cttgcctaca cgtgaccacc
    17461 gaaacggcca acctgatgaa aacgctcaag gccggcgggg cagatgttcg cctctgcgcc
    17521 tccaacccgt tgagcactca agacgatgtc gccgccgccc tggttcggca cgaagggatc
    17581 ccgacctttg ccgtcaaggg agaagacaac aagacctact accggcatat tgaatcggcc
    17641 atcgcccaca agccgcacct gtccatggac gacggagccg atgtggtctc ccatctacac
    17701 tccaagcgga aagacttact ccgcaacgtg atcggtggaa cagaggaaac caccaccggc
    17761 gtcatccgcc ttcgcagcat ggccgagaag aaagtcctga aattcccggt catctccgtg
    17821 aacgatgcgg acacgaagca catgttcgac aaccggtatg gcactggcca aagcaccatg
    17881 gacggcattg tccgtgccac caatcggctg gtgtgcggct ccacgctcgt cgtcgccggc
    17941 tacggctggt gcggccgcgg cattgccacg cgcgcacgcg gcatgggcgc caacgtcatc
    18001 gtgactgaaa tcgatccatt gaagggcctc gaagccgtga tggacggatt ccgggtcatg
    18061 ccgatggatg aagccgctcc ccttggagac ttttttgtca cagtcacagg caacttgaag
    18121 gttattcgcg gcgagcactt tgcggccatg aaagacgggg caatcgtctg caattccgga
    18181 cacttcaatg tcgagctgga cattcctgca ctggaaaagc tcagcaagaa aaaactcgcc
    18241 gtgcgctcgg gtgtggacca atacaccttg aagaacggcc gtcgcgtcag cctcttgggc
    18301 gaaggtcgcc ttgtgaacct agccaccgct gaaggccacc cctccagcgt catggacatg
    18361 agcttcgcga atcaggcctt gggggccgaa tatattgtga agaactacaa gaaattggaa
    18421 aagaaggtct atccggttcc cgccgacatc gataaagaaa tttcgcggct caaactcgcc
    18481 ggcatgggcg tgtcgatcga caagctcacc aaagagcagg tgaagtactt ggcctcctgg
    18541 gaaatgggaa cctagtttta tcggagaagg ccggagctgc tctcgtggcg gctccggccc
    18601 atccaccgag cagttctgag cgtctgagcg aactcctcat accactccag acgctcctac
    18661 tactctttct ctctgctctc tataactttc tctcagtcat accgaatacc tggcctctga
    18721 caagtattgg aggtcgattg tcatgaagcg actacaccac agtaggcgca ggtgcagctc
    18781 cggccaaata gatacagaaa acgtcaagtc tagacctccc cacgcctcac ttgctgaagc
    18841 acggggatag ctgacagaac atggcaaacc ttgcgcagac taacgtgaat tgaccatggg
    18901 aagttcgcga gatccttcgg cctcctgctg aatccgctga cgctcggctt tcgactcttc
    18961 cagcacactg tgcagcaact catcgctcag ctgggtcaat tgcgctgcag cgtctttcat
    19021 ctccgcatgc tccaccgccc gcgacaatac ttcggccttt gtcgccccct tcttctggcc
    19081 caagaagggc ttgatgatga gataggccac gtcacggaaa ggcatgaaac gcaagaatcg
    19141 gcgcagtagc ttgaaggtct gaatcgggta tcgggtgagc ttataaataa acagcttctt
    19201 cagcccctct tggcgaactg aattaatgac tgcccccggc aaacaggtcg gatcaatctc
    19261 cgaacactta aaatacttgt accaatcctt ggcgtcgctc accaatccac gcttgatata
    19321 ttcctgccac agaggcgtgc cccgatagac gcacagccga ttgaatccaa acgtgtccaa
    19381 aggcaatttc gaggcgaaat caaacgtctt ctgcatgtcc tcgacggttt cgtcgggatt
    19441 gcccaccgta aagaaaccgt gaacgatttc gatccctgcc ttcttggcgt ttctaacggc
    19501 ggtctcaacc tcggcgagcg tctgctcctt ctggaggcga tccagaattt tctggctgcc
    19561 gctttcgatg ccgaacatca ccgtccggca atgggcctga gccatggcgg ggaagaggtg
    19621 ctgggccacc gaatcaaccc ggccttcgat tccccattgg attgtcagct tcgcatcgat
    19681 gatcccttta caaatcgcat cgatccgctt cggctgcagt aaaaagtgat catcgacgaa
    19741 atagacagat ccgtacccat tcgcctcgag gtacttcagc tcatcaacga cgtgctgcgc
    19801 gctacgcgcc cgccacttcc cctcgttgaa aatcgggatg tcacagaaga cacagggcca
    19861 cggacagccc cgcgaagtct gcatggtcgt aaatcgctcc atcgacagca ccgccggcac
    19921 gtccaacggc atcgactcga caaagtctaa ttccaggctt tcccggtcag gaaatggcca
    19981 ctgatccaag tggcgctcca tcgtcctgtt ggggttattg atcaccttgc cgtctttcgc
    20041 ccaagtgaca ccgcccacct cttgcggatc ctcaagacga tcgagcaaat caagcaacaa
    20101 ctgctccccg tctcctcgac atacaaagtc gatttctgga cactggagct tcaccaaacc
    20161 cgcgttgaga ctggcaaata ctccgccgaa ggccagcttg acggtactat cggccgctcg
    20221 aatctgacga gctagaattt tggcataggg atagctggtg gtactgagga agctaagacc
    20281 aaccaaggcc ggcttccgcc gtttgatctc ctcgataatg aactcattcg gggtctcagg
    20341 atgcgcctga tcgaacatca cacattcatg gcccgaccgc ttcaataccg acgacaatga
    20401 cataatgccg atgggcggaa accccatgac ccggatacgc ccgttcttgg ctcgagtctt
    20461 ggccggaagg gcataaaact ggggatcacg cacatgaata agaaacacca gcatagtaac
    20521 gtcctttcat cgggacagta accggattcc tcagactgcc accagatcgc ttcgagagcg
    20581 gagaatccag gttcgaattg cagagaggcc aaatcactca ggccattcga cccaccggag
    20641 gcgtcataca gtaccacgtc tcgcaggagg gaaaaaggga agattctgac aaagagagga
    20701 gtacaccggt ggcatcggcg atcctcaacc gttgaccggc gatatggttt gcgcgctaca
    20761 aacgcaaaga gggcgtcgga tcagatccga cgccctcttt agaagagaca cctgactagc
    20821 gaccgagcgc cgccttcacg gcatggccga tctcagcggg attcttcacg actcgtacac
    20881 cagcagcttc tagcgccttc attttttccg tcgccgtccc tttgccgcct gaaatgatgg
    20941 cacccgcatg ccccatccga cgccctggcg gcgccgtgat cccggcaatg aaactaatca
    21001 ctggcttctt gacgtgcttc ttaatatatt cggccgcctt ctcttccgca tcgccaccga
    21061 tttcgccgat catcacgaca gcctgtgttt cagaatcctt ctcgaacaag ggcaagacat
    21121 ccacgaatcc cgtgccgttg acgggatcac cgccgattcc cacacaggtc gtttctccga
    21181 gccccaacgt cgacagttga tgaacggcct catacgtcag ggtgccgctg cgtgatacta
    21241 cgccaactac accacgttta tggatgaagc cgggcatgat gccgatcttg gcttcatcga
    21301 ctgtgatgac gcctgggcaa ttgggaccga ttaatcggac atcacgtcca tgcagcgcac
    21361 gtttcacctt caccatatcg ttcacgggaa tgccttcggt gatgcagata atcagtttaa
    21421 cacctgcatc agcagcttcc aggatcgcat ccgcacaaaa tggcggcggc acaaagatca
    21481 aagaggtatc gcagtcggtc ttcttcacag catcggccac ggtgttgaag acggggatgc
    21541 cctcaacttc ctgcccggcc ttgccaggtg tcaccccggc gaccatcttc gtgccatacg
    21601 ctttgcattg cgtcgcgtga aaggaccctt ccttccctgt gatcccctgc actaccactc
    21661 gcgtattctt attgacgaga atgctcacgg tcttctcctt tacgccgcct tgccggtcag
    21721 cttcacaatt ttctgcgccg cttcccacag atcattggca acctccaact tgaggccgga
    21781 ctccgccaat agtttgcggc cttcatctgc attcgtacct tggagacgca ccactaatgg
    21841 aacggtaatc ttcacttcct tggcagcttc gatcacacca tgcgcaatgc gctcacatcg
    21901 tacgatgcca ccgaagatat tgataaagat gcccttcacg ttcgggtcct tgagcagaat
    21961 ccggaatccg gctgccaccg tgtccttcgt ggctccgccg cccacatcca ggaagttcgc
    22021 cggctcgcta cccgccagct tgatgacgtc catagtggcc atcgcaagac cggcgccgtt
    22081 caccatacag ccgatgttgc cgtcaagctt gacataattc agattgttcg ccgtcgcttc
    22141 gatttccagc ggatcctctt cattgaggtc ccgcatgttc tgaacatcgt catgtttaaa
    22201 aatgccgttg tcgtcaaatg agaccttgcc gtccaatgcc accaaggttt tctccttggt
    22261 gataatcaac ggattgattt caacgagcgc cgcgtgtttt tccatgaata ggcgatagag
    22321 attgccgagc atctgcacga agggattgat gacagcaggt tccatcgtct gcaagcccaa
    22381 tgcaaaagcg acgttacgcc cattgtgccc ctgaaatcca acggcgggat cgaccgcttc
    22441 cttaataatc ttctccggag tcttttcagc cacctcttcg atttccatcc cgccttcggt
    22501 gctggcgata aacgtcggcc aaccggtgtc gcgatcaaca agaatgctca gatataactc
    22561 tttcgcaata ttggcgccct cttcgagcaa cagccgatga accttcctcc ccttcggtcc
    22621 agtctggtgc gtgatcaagg tcttgccgat caactccttg gcgattcctg caacagcccc
    22681 cttctccttc gtaattttga cgccgccggc ctttccacgc ccacccgcat ggatttgagc
    22741 tttcaccacg aagaccggcg tattcagttc acttgcccag gcagtggcgg cctcgggtga
    22801 ggtaatttcc ttgccccgtg gcaccggaac cccgaactgt gcgaacaatt gcttggcctg
    22861 aaattcatga acgttcattg aatcctcata agagctagtg gctcatgaca aatgacgtag
    22921 agcgtgactc ctagcggggt cggcttgccc ggaccagccg ccatcagcca ttcgccgtac
    22981 gctctggttc ttttcgcgta taggcgggga gaatcatcgg agagatcaca ccgctggagc
    23041 tgccgtgctg cttggcctcc gctcggaatc gtcgcagatg ttggagcgtg agcttccctt
    23101 gtggcatgga tccggcgcgc gatgccgctg tcaaccctga gcgctttcca aacaccatga
    23161 ggtccagtag cgaattcccc atcaatcgat ttcgtccgtg caatccccca gaagcttctc
    23221 cggcaacgaa gagattcttc acattggttt ccgagtttgc atcgatcttc actccgccgt
    23281 tctgatagtg caacgtcgga taaatgagga cgggatcctt gctgatgtca ataccgaatc
    23341 gctcaaactg catcatcatg gcaggaaaat gcttttccac cgttcccggc ccatgctctg
    23401 catcaagcaa cggcgtatcc aaccaaacac cgacacgacc ggacatggtg cgaataccac
    23461 ggccctcctc acactcacga ataatggagg aggagacgac atcacgggta tcgagctcat
    23521 tcacaaatcg ctcacctttg gcgttcacca gatggccgcc ctccgacctg ataccctctg
    23581 taaccaaggc gccgatcaat tgctcggggt agaccgcgcc tgacgggtga tactggaacg
    23641 tatcgatgtg atcgagtttc gcgcccatcc ggtaggagag gcacaaaccg tcgcccgtcg
    23701 cgccataatg gttgctggtg gggaatcctt gaatatgcag ccggccgata ccaccggtcg
    23761 cgagaatcac agacttcgca gccacaacca cctgcctgtg attgtcgaga tccttaaaga
    23821 tcgcccccgt gcaatggccg tggtcatcac tgagcaactc cactgcggcg cagaattcga
    23881 gaagttggat cttttgattc agcacctcgt ctttgaggac tcgcatgatc tctaggccag
    23941 tgtagtctga gcaagtcaga agacgtggct tagagctccc cccccccttc ttgacgtgca
    24001 gattgccatc ggcctgccga tcaaaaagca cgcccagttc aattaaccac ttggctatgg
    24061 aaggcccatc ctcgaccatg accttgagta attcgtggtc gttctgcata tggccaccct
    24121 tgagtgtgtc aaggaaatgg gtgaccggag aatcttctgg cgcaacggaa atctgcatcc
    24181 cgccttgagc catcacacta ttggagtccc caagtcgcag cttcgtggca agaatcacct
    24241 tcgcgccggt accgtgggca tgcaacgctg ccgcacaccc agcaccgccg ccgccgacga
    24301 ccaaaacatc cgttgtgtag tgtggggtca gatcaatatt ctcagcgatt gagctatcac
    24361 cttcgagaag ggcagcgagt tcgacgaccg tcttgtcgtt cgcgttcggg ccaaatcgaa
    24421 tcggacgata ggcgctggta cggtaatcag gatgatactt atggatcagc tggtcgcgat
    24481 cggcagggga gaacttcggg atagtctgtt tacggcgagc gtcccgagtc tggtggacaa
    24541 tctgttggag tgcgtgaata tccatggttt cactttcagc acggagcaca gtcttcggac
    24601 atatctgccc gagaccggcg gacggcacga ggcattactt cactgtcgcg cagtggtcgg
    24661 ccagctcctg ttcgttcatt gcgagcactt tgttccattc atcgttgaaa cggccgtctt
    24721 gaatttcctt gatacggata tccagcccct gcggcttctc tgtgaaatga gcaccttgcg
    24781 cccggctcac gtacagggcg accagattgg gggcgatgtc ggcaatacac accggtgtgc
    24841 acatcccaca catgacgcaa tccatgaaca tctcggagac gctcttgaaa tcaccgaaca
    24901 ctgccttcca caccccttca cgcacatcga tcttctgagg gcacgcctcc gtacaggcat
    24961 tgcaattgcg gcaaagcggc gcttccggat agagattgaa gaggtcttgc ttcggatctt
    25021 tgagcgactg gatgtcgtac agagccttac gagcgggaaa cggcggcatg atggtgaaag
    25081 acatgccatc ttctacagcc atctggcagg ccaagcaggt ccggaccttg ggatcatcct
    25141 tggttcgata gtaggtcgcg caggctccgc agaatccgcc gaggcagccg gctccacgca
    25201 ctacttcctg gcccgtatgc cacaatgcct tgatgacggt gataccttcc ggcacttcat
    25261 acttcttacc ggagatttca atcgtcacca ttttgtggcg caacacttct ggctggtcga
    25321 tcacgttggt gtcttcttgt gccatgtttc tctcgtggac ggctgaaagc caggcgtgag
    25381 gagattcgct tcccccctca cacctgacgt ttaattaacg gatacgccct tacgcaattg
    25441 cgtcgatgat agcatttagc gtggtgcttg ggcgcatcgc cttggacgac ttcgcgtcat
    25501 cagggtggta gtacccacct gtatcgaccg gcttaccctg agccgcaatc agctccccag
    25561 atatcttggc ttcgttatcc gccatttcct tagcaacctt gacgaagcgc gctgccaaat
    25621 tcttatcctg cgtctgctgg gccaacgcct gcgcccagta gagagccaga tagaaatggc
    25681 tgccgcggtt atcgatttct ccgactttgc gcgccggcga tttattgctc tccaggaact
    25741 tggcattggc ttgatcaagg gtatccgcca gcattttcgc cgcgggattg ttcgccactt
    25801 tggccagatg ctccagcgag gcagccagcg ccaggaactc tccgagcgaa tcccaccgca
    25861 gatagccttc ttcctggaac tgctgcacgt gcttcggcgc ggatcctccc gcaccggtct
    25921 caaacagacc gccgccgttg agcaacggca cgatcgaaag catcttggcg ctggttccga
    25981 tttcgagaat cgggaacagg tccgtcagat agtcacgcaa gacgttcccc gtcacggaaa
    26041 tcgtgtcctt gccttccttg atccgctcca gcgagagacg agtcgcatca gccggcgtca
    26101 tgatccggat atcgagcccg ttcgtatcgt gctctttcaa atactgattc accttcgtga
    26161 tcagttgcgc gtcatgcgcc cggtccttgt tcaaccagaa aatcgccggg gcgccggtcg
    26221 cccgcgcgcg agtcacagct agtttcaccc agtcgcggat cggggcatcc ttcacctggc
    26281 acatacgcca gatgtcccct tcttcgacct tgtgctccag caaggctttt cccgacgcat
    26341 cgacaacgcg aatcgtaccg ttgcctggca ccttgaaggt cttgtcatgt gatccgtact
    26401 cttccgccgc ctgcgccatg agtccgacat tgggcacgct gcccatcgtc ttcggatcaa
    26461 gggcgccgtg cttcttgcag aaatcgatca cctcgcgata gaccggggca tagctggcat
    26521 cgggaatcac gcacttggtg tccgccagct tcccgtctgg tccccacatc ttgccactgt
    26581 cgcgaatgac cggcggcatg gaggcatcga tgatgatgtc gctcggcaca tggaggttgg
    26641 tgatcccctt gtcggaattc accatcgcca tcggcggacg ctgcttgtac acagcctgga
    26701 tatcagcctc gatcgccttg cgctgatcat caggcaaggc cttgatcttc gcatagagat
    26761 cgccgagccc gttgtccgga tccacgccca gcttcttgag cgtctcacca tgcttggcaa
    26821 agacatcctt gtaatagacc gtcacagcat gaccgaagat cttcgggtcc gagaccttca
    26881 tcatggtggc tttcatgtga agcgagaaca gtacgccttg tttggccgag tcctgaatct
    26941 gctcttcgag gaatgcgcgc aacgccttga cgctcatgaa ggtggcatcc accacttcgc
    27001 ccgcctgcaa ggccactttc ggcttcagga cggtcgtctt gccatcttgg ccgacgaact
    27061 cgatccgcac ttcagtcgcc gcggtcgtcg tcagtgactt ttcattcgaa aagaaatctc
    27121 cacccttcat gtgactgacg tgagacttgg aatctgaact ccaggcgccc atcttgtgcg
    27181 gatgcttcct ggtgtgcgcc ttcacggaca agggcgcccg gcgatccgag ttgccttcgc
    27241 gtaagaccgg attgactgca ctgcctttga ctttgtcgta gcgggacttg atatccttct
    27301 ctttgtcgtc cttcggattt tccggatact cgggtagctt gtagccctgg ctctgcaatt
    27361 ccttaatggc tgcctgtaac tggggcagcg aggcgctgat attgggcaac ttgatgatat
    27421 tggcttccgg tttcttggcc agttcgccca attcggacag cgcgtccggc tgccgttgtt
    27481 ccggtgtcag atgctccgga aacaccgcaa ggattcgacc ggccagcgag atatcccgca
    27541 gttccaccga tacaccggct gccttgctga aggcattgat gatcggcaag agcgaatagg
    27601 tcgcgaacat cggcgcttca tcagtcttgg tatatatgat cttgtcagct ttcgtagtca
    27661 tgattctccc tcttatcccc tatgagttag ttcagcgcat tcaacgccga acccgccttg
    27721 aaccaagcga tctgctgctc agtgatgctg tggttcgttt ggatggtcag agctttgcca
    27781 tccgccttgt gtatggtgac ctgcacaggt ttgcctggag ccagattgtt gaggcccgtc
    27841 acgctgatgc ggtcttgctg ctcgatcttg tcgtagtcct tcggatctgc gaaggtcaac
    27901 gccacgatac cctgcttctt gagattggtc tcatgaatcc gagcaaagct tttcgtgatc
    27961 actaccttca cattcaaaaa tctcggagac atagccgcat gttcacgact gctgccttca
    28021 ccgtagttct cgtcacctac tacaactgac cctatgcctt tggacttgta gtcgcgtgca
    28081 atctgagcaa tcgtgagatt tgcctcaccc gtgagcacat tcgttcctct gcccggctct
    28141 gatgtaaagg cactatttgc gcccaaaaac atgttgtcgc tgatcttatc gagatgccca
    28201 cggaacttga gccatggccc agctggagaa atatggtcgg tggtggtctt tccttttgtc
    28261 ttgatcagca gcggtagctt ttcaaagtct ttcccatccc agcgtgggaa tggttgcaac
    28321 agttgcaagc gctcgctggt aggcggtatg tccaccgtca acccctcacc gctttcagca
    28381 ggagccacga acccctcttc acctttcgca aaacctttgg ctggaagttc ctcaccttgt
    28441 ggcggctgga atttgaactc cttgccgtct gcacccttga gcatctgatt caccgggtca
    28501 aaaccaagat ctcccgtaat agcatacgcg gtcacgacct cggggctcgc caagaacgag
    28561 agtgtttcgc tgattccatc attgcgacca ggaaagtttc ggttgaagga gctaacgatt
    28621 gaatcggcct ttcccttcac cccgtcggcc cgcttccact gcccgatgca gggaccacag
    28681 gaattcgaga ggaccgttcc tccgagcttt tcaaacgtat ccaaaaaccc gtcacgcttc
    28741 atcgtatgat aaatgcgctc ggatccagga gaaacaagaa acgatgcctt cgcttttaaa
    28801 ccagctttca agccctgctc agccacatgg gcagagcggc tgatgtcttc ataggaagaa
    28861 ttggtgcagc taccgatcag tgccgccttc agctcgaccg gatacccctt cgcctttgcc
    28921 tccgccgcca actttgaaat cggccgtgca agatccggcg tatgcgggcc aactacatgc
    28981 ggttcgagcg ttgacagatc aacctcaacg atctgatcgt aatatttctc cggcgattga
    29041 tagacttccg gatccgccac aagcaggtct ctgtgagatt gagcaaaggt cgccagatca
    29101 gcgcgatccg tgatggtcat gtaggcgacc atcttctcgt cgaaggggaa cacagaggtc
    29161 gtcgccccaa gctccgcacc catgttacag atagtgccct tgcctgttgc actgatcgtt
    29221 tcggcccctg ggccaaagta ctccacaatc ttgttggtcc cgcccttcac tgtcagcaga
    29281 ccgcacaggt agagaatgac gtctttcggc gaagcccagc cattcaattt tccggtcagg
    29341 cgcacgccga tcaatttcgg atgaagaacc tcccacggca aacctgccat cacttcaccc
    29401 gcatctgcgc cgccgacacc gatcgccagc ccacccaagc caccaccgtt cggcgtatgt
    29461 gagtcagttc caatgatcaa acagccgggg aacgcatagt tctccaacac cacttgatgg
    29521 ataatcccgg ctcctggctt ccagaaccca atgccgtact tctttgccgc agaagcgagg
    29581 aaattgtaga cctctcgatt ttcgtccatc gcgcgaagca ggtccttctc ggaacccatt
    29641 tcagcacgaa tgaggtgatc gcaatggatc gtgctcggca cagccgcttt ttccttattg
    29701 gcctgcatga actgcagcat ggccatttgg gccgtggcat cttgcatggc cacacgatct
    29761 ggcctgagcg ccaacatcgc cttgccccgc tcccagatct gcgcgtcgaa attatctgcg
    29821 tgcgagacca agattttgtc agccagggtg aggcctcggc cgaattgctt tctggccttg
    29881 gcaaacactt ccggcatctt ggcatacagt ttcttggcga gatccatcga catgacttat
    29941 ctccttaaaa tatgtctgtc accaaatcag caccatcagc cggccgggaa catgacattc
    30001 caacgaccgg attgacgagt catgtttgac gaccgaccat tgtcgtctta cttcaatggc
    30061 ggaacttccc gccgcttcgg tgccgcataa ttcacaaggt agtcgaataa acggatcagc
    30121 cggctatcct ggcgtttttg atcaacccag tggccgatca atccgatcgt ccgcgagaga
    30181 atgaagaacc cgttcaagct atcgacaggg aatccgatat cgaccagtac ggctgccatg
    30241 gtcccatcga cgttcagaat cagattatcc tttttcacgg aggtaatttt ctccacctcc
    30301 agtgcgaagt ccaggcatgg tgtcttaata ttcaagctct tcacgtaccc aaccaattcc
    30361 ttcactcgct tgtccgggtt gcgcaaactc ttcacgcgat gaccaattcc tggaaccggc
    30421 ccatggtttt tcttcatgta ggccaagaat tcgtcgaccg acatcttgtt atcgacagca
    30481 tacttaaaga accgcccggc atcagtaacc gcaccgccga agcgcggacc aatcataatc
    30541 atgcccgcgg caactgattg cgacagcccg atcccggcac aagccgcaag gattgtcgcg
    30601 tatgctccgc tcacgcaagg gccgtgatca gcggaaagca tcatgatgcg cttaatgatt
    30661 tcggcttcct gtttcgagat cagccgcttg tcccacaaaa gaccgatgac atgaggaatt
    30721 tcgtagcctt tgttgatgag ctcagaggca ggataaccgt cgtagcaggg ctcatcgccg
    30781 cggtcatcgc tgatggtcgt acggatcagc ggcgagacca tgacctcgtc agccttcatc
    30841 gcctcttcga tggtttttgg caaccggggc aacgtagccg gctcgaccgg ttccttgatc
    30901 tggcctgact tcaagagatc ttgataggtt tccttgatgg caggaccaag ggcgccaaaa
    30961 gtgggcggca cgagggcgcc ggctttcttc agcgcatcgg acttggagcg agctgatcct
    31021 tcacccttca tcccctcttt cgcgccggca tgaccgaact tcatcccctt cggcaaactc
    31081 tcctgacaaa atccggagac cacggcaatc aacttcaccc ggcgcttttt cgcgccatac
    31141 cattctgcag cccgctcttc gagatcaccg cccatttcgc cgacgatgac aaccgccttc
    31201 gtttgcggat cgttttcgaa catctcgaga taactgacat aatcggttcc aggataggca
    31261 tcgccgccaa taccgatggc cgtcgtgatc ccgtcggcaa actgagagca gatccagatg
    31321 atttcgttcg agagaccgcc ggacttggtg atgacaccga acgatccttc gcgataaagc
    31381 ttggatagaa caagattgtc gaacgcaccg ccaatcacgc caagccggca tgctccggcg
    31441 gagatgatgc caatggatga gggaccgtta aagaccttcc ccaacttgcg cgcatgagcg
    31501 ccaagaatct tggcatcttt ttccggcacg ccctcggtga tcattgagac caccttgata
    31561 tgagaatcgt ccagcgcctc aataccgccc ttcatcgcac ggtctgcgcc aatgtacacg
    31621 agactggtat tcagggtcgg atggtgcttg gtggcttcag caatggactt gtagaccgga
    31681 atggcgatca gaccgctgcc gtatgggatc tcattgctct tgccagcatc gggtggatag
    31741 acaaacgcct caacgttgag cggacgttta atgagataac agaactcggc catgcggcgg
    31801 gcagcgttga caccggcttg accaccctgg atcaccacac gggtgtcttt gtttgccaga
    31861 atactcatct cgggatctcc ccttatcttc ggttggtcat aggcggcagg ctacttcgct
    31921 tggagtgcct tgtcgacaat gtcggtgagc ggtgtattgc ggtcgaagac attgatgtca
    31981 aagccttctt ctttcaacgc gcgcatcgca tcaagcccct ctttttcccg cggcccaccg
    32041 cgacgcaccc aaatctttac gctcttcaac ttcccttcgg acttcgcctt cctgaagccg
    32101 ttgatgatcc ctccgaacgt cttcttcacg tcggtgaaat tggcgatcgc accaccaacg
    32161 atgatgttcc ggattcccgg cagagaacag accttttccg tgaggacctc gacggcccaa
    32221 tcgggtggat caccggagta ttcggcatag ttggccaact tgccgccacg cgccacgacc
    32281 gcgtcggagt agtacacgct ggcaccaccg cccgccggca acatcgctgt atccccacca
    32341 ggaatttcta taaatttaac tgagccttta atcttgctgt cgacagccat cacctcgagc
    32401 tcttgcttgg aataggcacg gccaaattcc gccgcaaaag cgaagttcca gtccggatgt
    32461 ctaaatttgg catcgccgtc gagcaatgtc acggcatcaa gcgccaccag ttcttcatcg
    32521 gcggcacgaa ggacgaccgg attcacctcg agatactgcg catcttcact gtcgaagcag
    32581 gcaaacattt ttcctgcaaa ctcagccatc ttcttcgtga gcggcccgcc aaatcctgcc
    32641 tctttcgaca gtttttcgag ggattcagga gaaggagctt gtccgatctc aagacacaac
    32701 cgcttaacac gttcccaatt tgattcgact tcgatcccgc cacaattggc aaccaggatc
    32761 tcactgcctt cgcgcgttga tttgaccgcg caataatact cttccttgtg cgggatcatc
    32821 tcagagacaa tcacctgagt cacggtgatg ctgccgacct gacggccaat catctcttta
    32881 gtggcagctt cagccgcctt gaagtccaga tcgatcttca ccagccccaa cttgaagcgc
    32941 gacccgagag cctcgtgtgc cttcgcaaca agcttggact tcttcaacca ctcattggct
    33001 tgacccagcc tggtcaactc gtctacggag gtgacgacca catagttggg aacactgatt
    33061 ccccacttct tcatcagccc catcccggga ccttccagca ctttcgccat gattgacgac
    33121 tccttggtgt gtgatagggc agagaaacgg agcggtagag taccgaattc tgctgattag
    33181 ctcaatcctc caaaaggccc aagctgcgag cacagaagtc ctaccataat gagcactgaa
    33241 gattcatcac cataaataat tgataacgtt attattgttt ccgacgcttc aaggtttggg
    33301 gttagcgaga ctattcaggc cttgtggcgc gccgacgccc gtttcgctgg catgtgggac
    33361 agtaaaacga acttcgttcg ccgatagaac gacagatcac agcaccgcag gcattcgggc
    33421 agggctcccc ggctttggca tacaccagat gcctgcgctt gtactggcct tcggtcccat
    33481 ccggcgcaaa ataatcccgc acactcgacc cacccatggc gatggcttcc cgtaacacct
    33541 cccccatgac ttgatgcaac cgcgtgatcg acttccctgg aatcgaattc gacggctgat
    33601 tgggatggag gcgggaacgg aagaggatct catttgcata gatgttgccg atccctgcga
    33661 tgacctgttg acgcatcagg agagacttta gccgactccg tgtcgcccgg agcacacgca
    33721 ggaattgctc atgactaatc gtaaggggat catggccgaa ccggcgagcg acatatcgat
    33781 ccagtcccgc ccggtcaagc agcgataggc gaccaaaccg ccgcggattc caatatctga
    33841 tatcaggctc acaacaacca tcgaggtgca ggataaagtg ggtatgttgg gggtgcttgg
    33901 tcaaggtgga tcggaacagc aacaatccgg tcatgcctaa ctctgcgacg agaaatctcg
    33961 gctccttgcc gcagagaaag tgaagaatca cgcttttccc cttccgctcg acccctgcaa
    34021 ttttggcctg acgatactga tccagagaag gcaacccttc tcgcacgatg tcctcacggc
    34081 caatccagca atcacggact gtagctccga cgacccgttc gcgcaattga cgcgcggcga
    34141 cttcggcttc tggcaattca ggcatcgagc attctcgagg tggcagagag aacctactga
    34201 aggcaaatta cgccatgggg ccgaggcaaa atgcaactcc gacctgagat agtccaggag
    34261 acatcaattt ggatgactat aacgagagcg tccggagagg gatgaggaac tgacgaagaa
    34321 ggacaaatcg gaatgtccct gacagcgcag cttctaagac gttgatgaat tcacttgggt
    34381 ttgccggcgg cgacaaacat cctgaatcgt ttcttggagg cgcggcaacg gcatgccgcc
    34441 tggacgcctg gtcaacacaa gcttcaccgc atcagcatac gccatccctt ccacgcaaca
    34501 gagataagcg atcaccacag aaaccgaacg tcccatgccg acccgacaac acaccatcaa
    34561 tcgattgtcc gccacatgag ccgcgatcca ttccacagcc tcgtataaag ccgcagctgt
    34621 cggttcaccg aattccttta acgggatctt ggcatagata acccgggagg gaactgtcac
    34681 attttgttct tcagccacca tgagaacggc acttacctgg gggggaggat tcctggcgtc
    34741 atcggcattg ccgaccagaa gacgtttgtt gatcatgtgc atcgtgtcgt tagtataggg
    34801 ggcgcgtgct tccggtcaag tgaatcctcg gaattcggac aaggcggttg aacatcactg
    34861 aagccggtgc gtatactcaa ctccgctatt cgaggaggaa ttgatggctc acacgtcgct
    34921 tgaagcggta gaaaaacggc tggcgagtgt ccagtgcgca atctgtaaga ccaatacctt
    34981 tggcgtcgac cggcgcacat tgcagccaga tggggaatgt cgggcggtct gcttgaaatg
    35041 tcggtacaat tttcccgtct acacagatat ggagttttac cttcggactc aaccggatgt
    35101 accctatcgc ctaaaagaga tttcttgccc aaactgccag catcgaggcg tgaccttgga
    35161 ctttcggatc accatgtccg tccgcgaggc gatctatttc gtcacctgca acgcctgcca
    35221 aacacagttc cctgaacgct cttcattgga agcgtttgag taacccttcc gagcgcagtt
    35281 tcatgtatac taagcttatt atgagcacca cacaaaagcc agactcccag aaaaatccta
    35341 ctccggtcac tcagactgaa acgaagcccc ggagggaaat tccgcttatt tcagttccaa
    35401 acgatcggga catgattgca gcggaaatgg ccgaactctt cgacgaagat cgagtatccc
    35461 atcagcacgg ttcctcagaa cctgaggcgg actagatctt cacgcagagc gaacgatctc
    35521 cacggtgccg gacgagataa tccggcccac catagccggt cgcccttttc gctcctttct
    35581 gacaatgtcg ctcacccgaa tgatggcccc gcgatgccag ccatcaaagg cagtgttcga
    35641 cattgagctt tccttgtcct ggcgtccaat ctccgacctc cccgtttgat cgcacaacca
    35701 aacccgtttt tcgcctttca tcacgcgcaa ctcactcacc acgcggtggt agtccggatc
    35761 tcgtggcacg agcgtttttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35881 cgcaactcac tcaccacgcg gtggtagtcc ggatctcgtg gcacgagcgt ttttccgtcc
    35941 ttgcgaaaaa tcacatagga aaatttgagt gcatctttga gaagcccgac ttcccgatca
    36001 acttgagcaa tccaggctgg agtcttccac ttccgttctt catggcacca gtcatccggt
    36061 ttgagtagtg caggacaagg agcgtcatgg agacagggcg caaagacaga acagtgctgc
    36121 cctgccagaa ggttgtcccg cacctgatgc aattcgcgcg aagcaggccg cgtggccggc
    36181 tcgataagca ttagactccc ttcaggggcc aagtaattca ggagttcccc tatcaacgca
    36241 gtccgttgtc ccacggaatc catactcccc acgaataact ctgatagaag attctgaaca
    36301 ataataagct ggtaaccatt cacgccaccg ccgtcacgga tagcggaggg cagggaacgt
    36361 tcgaggttac actccacggt gttcaacggt gggatggact gatcccgctg ctgttcacaa
    36421 tacgcccgcc aaatatcagt agacaccctt aagacatgag gtgaccgatc aacggctatc
    36481 atacggaggg tagacggcgg cctcgcagac tttgaaaggc accaatcgag cactcccagg
    36541 actcctgttc caggcccgcc accgaggtcc aatactcgaa aatcctggtc ctgagcgacg
    36601 ggaagcaccg gctgcagttc gtccaagagt atctgcactt tggcgagatt aacaggcaga
    36661 tagtagacca agtaaccggc acacaactga gggtcctcga agtaaaccct gcttcccacc
    36721 tgcccatttc gtgtaaacat ttctgaaagt cccgacaccg cctcaattat tgattgggat
    36781 ggcccctccg agtgctcaag gagatttact ttggccacct tcgtgatagc cctcaaaact
    36841 gccgatgata gttggtacat agacgaaccc tcggttgacc ccccgagcag gctggggtat
    36901 tctcatgaga gaaggagcac ttcacatgac tgaccctatt ttgcaagcat atctggaagt
    36961 ggagatggcc atggagcgct tcacgcttgt cctgcatgac catgtcgatc atctccgcaa
    37021 gactgaagcc cctggctcag acaagctgca ccgtatggcc aacggcacca aagccatgcg
    37081 cgatagcgca tcgatttacc tatcctacgc caagtacgtt gctcacggga tgccggaaag
    37141 ccctgatcta gtcgaggagg atcttcaggg ctgaggagcc gcactcaagc atctccctaa
    37201 atacacaggg cccgttcggt ataaccgaac gggccctgct gtttctaccc tgacgttact
    37261 tctcggccta aatgctgcgc atgaaatgcc ccatggcctc atggtcagca gctgatgcgc
    37321 ccataacttg tgaaatcgca acgttcgtgg acttctttcc ggtcagaccg gactcgactt
    37381 cgtccacgat caattccacc ggcatggact gcccaccgta cactctcggg ccaccgataa
    37441 tcttggcatt tgagaaaccg taaatagcgg ttgccacttc ttttgctaac catccgacat
    37501 agttgaattc aggcacaaca attaatttgg ccttcccgca gagtttgcgc aactcctgag
    37561 tcgggaatgg gcggagcgat cggatcttga tcagtccgat cttaatgcct ttttcggcgc
    37621 aaatacgaac cgcttcacgt gattgagcgg ccgcactacc tgaagcgata ataaccgcct
    37681 ccgcatcatc cacattctcc gccgtgagta gtccacccat gtactggttg atgtatttac
    37741 gggaacgctc cacagcagcc cacacttcct gctgccacac cgcgtgaatg ttgtaggcca
    37801 tgaaattgga cttctgaacg ggagcatcgc gagagagtcg tgccggagga ttctcagcat
    37861 caagcaccgg caccgcccca cgccaaggtt cgcgtggggg aagcttcatc ccacgatctt
    37921 gcatacgcac atagccacga gcatgcgtca caaaaaaccc atcgcagcac acgccgacgg
    37981 ggagggtcac atcattcttc tcgctgataa taaacccagc caacgtgaag tcgtacatgt
    38041 cctgctgatt ttcggcatgg aacacaatca tgccgcaatt gagcagataa gaaacttcga
    38101 tattgtcagg ttgaatggcc aacggtgcgt tcaccactcg gcaagtgaac atggccacga
    38161 ctggcaaccg atgccccggc caagaagcaa tcccctccag ccctctcagc gtccctggac
    38221 ccgccgttgc agtataacac cgcactcccg cccgtgaccc gccggcgata gccgccatga
    38281 cgccgacctc ttcttcgcca cggtagtact ccttgacgta cccttcacca tacaacacac
    38341 cgaccaactg catagtttca ctttgagggg taatggggta cgcaatggca agatcgacgt
    38401 ttgagcggcg aatagcctct tttgcggcct cgctgccagt gatgaactcc ttcgtgcgag
    38461 gagcttccag gaacatatac tcaggagtga caactttctg ccgctttgca tccgcgtgcg
    38521 gatccttctt ggtctcggcc ttcggagccg acacactcgt agtagggtcg ccctctggat
    38581 tggtcttttg tttgggttcc aatgcttcgc tcatcgttgc acctcttgac tgtatcgcct
    38641 tagcctaaaa ccttagcctt cccgcttcat ctgctcagct gagtggccaa atgtagccgc
    38701 gctgcctacg cctgcagcgg aaggcataaa ggtcatagga ttcgtatgcg tcttcccatt
    38761 atggaccttg gactgagttc cggtgaatcg cacgttctca ccgttatccg gcaacttaat
    38821 gcccatttcc tcacggacct tcttgaagat ttgcgctgtt ctcttcaatt tcaacgtgtc
    38881 cgaatcgcga attgtcagca tggcgtcctc atgcccctgc tcagcacaaa tggccatggt
    38941 gcagcagttc gtccctgcac gaatcatttt cagcgtaaga ttggcatagt gacagtgaac
    39001 gccgatgaaa atacaggcct caatcttgtt gccccagatc gtgagattgg ggtgattggg
    39061 attgatgacc tcttcagggt caattttggg gtacttggga cgataatccg gcatgggaat
    39121 aatcatcacc tcagggatct gtgccgcaat ttccaataca gccttcgcct tctcgacagc
    39181 atggttattc caagcccaca agacgagagg tcccggaaaa atcgttgcat tcgggcttgt
    39241 cagcattttc cgagccatct cttcaatcac tatgtcttcg ttaggttcca gcagcccata
    39301 gtacagtcct tcgcctggat caggcagcgc gacccctaat tgagctgctg atggaggatg
    39361 gaatccggct ggccccggca cgataattct ctctcttgta tcttgcgtca ttcccacgcc
    39421 cactcccctt tcttacgctt acccgactac tgggctcgcc aagaccgctg tcgtactctt
    39481 atcgctatat gtgatgttat cggtcaacgc agccatcggt aattgatcaa tcatgatcat
    39541 tttaatagca ttgttcttcg ccatgttgtc gcagacccac acacattgcg cgcatccttt
    39601 acaacgatct acggccacat atgcagaccc gtacttaaag cccttctctt tccccatctc
    39661 ctcgctatac tgaatggtat tagcctcagg acaatactga gtacagagct tacagctctt
    39721 cgaggcacaa atttcaacat cgatatgggc gactaaatac attggaactc cttcgtacta
    39781 aatcctggtt aggcgctcac agtcgcacgg atttccgacc gtttgaccgt cggtgcagcc
    39841 caaccgtggt cgactgcgta gttccagccg gcctgaataa ccgcgacatt cttgtcgatc
    39901 aactcctgct tctttttaaa ttttcgctcg acgacactgt ccagggccgc ggttcctccg
    39961 gagaccacaa atcctttacc aaggaaccga tcctttaccg actgctccag cgcttcaaca
    40021 gtagtcaacc cggtgatcgc tccgatgcaa cccatcaagg ccatattcgt gcacaagtcc
    40081 atgcccgcca cttcgaggga tagcttagta gcgggaaaat agtaaagcct tgcacgacgc
    40141 tcttctaact cacgagcctg atcccgatga agcttcatcg gaccatcatt attgatcaac
    40201 gcgattccgt cttccttcaa tccgaagtaa aacggcatcg tatacgattt tccgtgagta
    40261 atgacctgtg gatggaaaat gataatgatg tgcgggaagg tgatctctcc gatttcgtag
    40321 atgggctcgt ctgatactcg cacataactc tccaccggag ccatgcgctt ttctgatccg
    40381 tagaatggaa ctatcgtact ttccccaccg gcattaatca ccgcagtact caggatgtgc
    40441 gagccggtca caaccccctg gccacccaca cccgccatcc gaatgttgaa acgtttcgcc
    40501 atgctcaatc ctccttaaac gttgctcagg ctttcggaag cccaggagcg ggagcagccg
    40561 gcttcgggag aaattccttg tagccatacc gttccgtaag aaattgcttc gcctcgtcgg
    40621 taatatattc ggagaactga tagcgatcgt tttcaacgtt cttcgcgtct tccatgacct
    40681 tgtcggtcgg aatggcatac tcaatgttgc aggacgtgta cgcctgaatg taagtcgatc
    40741 ccacctctct ggcgatcaac acggctttct taatgacgct ctcaacacgg cgaggattgt
    40801 tcggcaccac cgtcgcgacg taggcgcagc ccgcaacctt cgccatctgc agcatatcca
    40861 tcttctcaaa cttcttcccc aacggagcca tcttgagcac ggcgccgcga ttcgtcatgc
    40921 cgctctcctg cccgccggtg ttcccataca cttcattgtc tagcatgatc gtggtgaacc
    40981 gttccttacg gaaccaggag tggaggacct gctgaaatcc aatatcagcc gtaccgccgt
    41041 cacccgccat gacaaccaca tctttcggct tatcgccaaa acgaaggcgc aagccacggg
    41101 acaacccgct agccacacca ttctggtcac catagttgcc gtagacgaag gggatggcgg
    41161 cttgcgaaat cgccaagcgc ccgcaaccgg ctgttccaac agtgatagta tcttctggat
    41221 tggggaaggc aatcatggcg agtcggataa agagggtcat cgcacaaccg gcacacatgg
    41281 ggtgctcttc aagcacttct ttgaaggagc ccatctgtga aacggacacc tttttgccga
    41341 aaggaccatg ctcaaccatg tcccgatatt ctttcggcat gaacttttca aagccgcttg
    41401 aaaacttgac gtaatcaagg ctcataagga ctccctccct tttataagct gaggactttg
    41461 ttcggaccaa caacagcggc cagaaaaagc caggtgcaga ttatcttgac gtcatattgg
    41521 actaaacacc gaactgtgct cctgacacgg aacggaatgt tagtcttctt acttcgtgta
    41581 gtcgccgagt gtaacaaagc ccgaaaaaat ctgtcaatcg caaccagcac ggcaaaagca
    41641 aaagatccca tcacttcagg acaatggcga gtctatagag tccacaaaag cattgcaact
    41701 ttgcagaagg cccacaccgg acgaaagaag gcctagtaga gatacccgtg ctaggccctt
    41761 ggccctaaat ccgcttgaac gcgaagattt tactcctctg caagtccgat ctgtcacgca
    41821 attcagttgc agttcacccg agaagcgggt aaacattgtt gcttaacgcg tttcggtcta
    41881 cactccgcct atgcctatac gagtactggt cccgggagaa gaacatggcg agcagcatgc
    41941 gggaggggtg cacaagcgac caccaattcc ggatggctcg ctcaaggccg agtgcccaaa
    42001 attcatgagc catggcccct gcggaggcgt ccgcaaaggt ggattctgcg aggtctaccc
    42061 ggaaatgacc tgtccgtggg tcacgctcta caataaattg aacgaactgg gccagctgga
    42121 gtggatgaag caagtctgac aacaagaagg tggggggagc gggtgacggc gaaaggcagt
    42181 aaaaagcgga agccagctag ggcaacgaca aagctgggat acaacgagcg gcacgccaag
    42241 agtggaatct ctgctcctct acccgacatt gagacattcc ccaatcaata taagggatac
    42301 gaaattacaa tcgtcatccc agagtacaca gccatttgcc ccaagaccaa cctgccggac
    42361 ttcggaacta tcacccttca ctataaacca gacaagcatt gcctcgagct gaaggctctc
    42421 aaaacgtaca tccatgccta cagaaatgta ggaattttct acgagaatgc cgtcaaccgc
    42481 atcctccagg acatcgtacg agcctgtcga cccatcaagg ccacggtaac cggtgaattt
    42541 gctgctcgtg ggggactgcg gagcgtaatc gaagcaaaat atccggcata aattgcctac
    42601 tcaacagcct ccactttacg ccgtcgacgg gctacttatc ccgcttcacc tcatcaccag
    42661 cacacccatc gcaactatcc cagtaacact gcggcagctc cctgcgcgct acgcattacc
    42721 aatgtttctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aagaagaatc
    42841 tcggcgacct cagcctggat gattttgcga actacacagg tgtccggctc cgtggaaacg
    42901 tcctacgcac ccacaacttc aagcagcaga atcctccggg cgcagaatcc ggcgcggtcc
    42961 aaaacaagag cgagctcgga ttcccggaag tgttcagcct gttcactgcc ggcaccttga
    43021 gcaaaaacat tggggttttt gccgagctgg aatccaatct cgaagaaggg acaaccggga
    43081 tggaacgagc gttcattact ttcaacaatg ttggaggtga gaatatcgcc aacatccgca
    43141 tcgggaaact cgatccttcg gccgtattct ccttttccac cctccggcaa caactcgaat
    43201 ttatcggtga gagcacaaat tcctccaccg atgcggtcca gcgggcaggg ctcttcccgc
    43261 ttgccacagc cgctaaattc tacggtctgc gcgatcgctc cggagccgtc atttccccct
    43321 atgccccctc gctctacaac gcagtggcgg aaaccggcat cgaagttcgc gggcgtccgt
    43381 tcggcgactg gttcatgtat caggtcggta tgctgaacgg ctcgaacgag ggctttggag
    43441 attccaacaa aggcaaagat ttttatggcg ccatccgcct tgattacgcc cgctcggcca
    43501 acttttcagc aagcctttcg ggatttgcct atctcggaaa cagcaatgcc accgtgttcg
    43561 acggcacacg cactgtcgat gtgaattgga atcgctacgg cgccgccgcg cacctacgct
    43621 acaagatgct ggatttccat gggatgtaca cactggatcg aatcaagcat gtcccaacag
    43681 cggccttgag caatttcgac accaccgcca gcggagtgac cgtcgcgctg gatgcctacg
    43741 tcacgaaccg aaccctgctc tcgctacggt acgacaacat ggatgccggg ggagatctga
    43801 cccagcgcac atcccagagt ttcgccggca tgcaactcaa gcactaccta cgctcgaatg
    43861 tggccgttta cgcccgacac gacctcaatt tgcgtcgtgc agaagagggc aatgcggcag
    43921 cccacaacct gagacatgca gtttttgtgg gaatcgatat ctcttactag accgggaaga
    43981 atcatgcatc gccatgtact ccgatatctt gcgatcctca tcgtgacggg cttctgcgcc
    44041 acgaccgcgc aggcgctact gccgaaggac gggcgggata ccattacaga ggggcatcga
    44101 atctatgatc gggcctgcgc ctggtgccat ggcagcgaag ggaaagggga cgggccatcc
    44161 ggctggtcca tcggtcgcta cgcagcccct cgtcctcgcg acttcaccgc ggagagctac
    44221 aaatttcgaa gcaccccctc gggtgaactg cccacggatc aggacctttt cagaaccatc
    44281 acacagggta ttcccggctt catgccgtcg taccgatcct tgaacgagca ggaacgatgg
    44341 caggtcatcg cctatctcaa atcgttgaat ccggcgttcg agcaggaaca gccgacgccg
    44401 atcgcgctcc cctctccgcc gccccttccc tccgggaatg gaatcacaaa cggccgggcg
    44461 ctgtacgcca agtacgagtg tcggacctgc cacggggaca acggtgcagg tgatggaccg
    44521 gaatcgaagg ccgggcacct gcgggacgcc cacaaccttc ccatcaccgc cacggacctg
    44581 accgacccat cctcattcaa aaacggggcg acgcctcacg atctctatcg aagcatcatg
    44641 agcggactcg acggcacccc catgcctgcc tatgccgatc aatttgccgg acaggaagaa
    44701 gctacatggg atctggtgtg gtacctccag tccctatcag gtcagccggg gcgataattc
    44761 ggcaccatga agcacccaca tttcatacaa cctgcgaatc ggacgcccaa cggggaaatg
    44821 gagatgcggt acatgaaacc gatactgctg accgtgacgc tgtgcttgat gctcggccag
    44881 agcgccctcc tgagagcgga atctcccgcc ggcacactgg tgggccgaat cacctatgcc
    44941 ggcccgatcg aatcagggca ggtggtcgcg gtcacacgcg attcgtcgtt ctgcggcgcc
    45001 accaccgcca tccacaccgt gacggtgaat gcgaagacgc gaggccttca aggtgcagtc
    45061 gtcagcatcg acggcgacac agtccccatg gccgattcgc cacgccgccc gctagtcctc
    45121 accaataccc actgtgcctt ctcccctcgt attgtcgcag gtgaggttgg acagcaactg
    45181 gagatccgca acgacgaccc cgtcatgcat aacacccaca tttccatggg gccgagaacc
    45241 tttgtgaacg tcgccatgat ccccgcgagc cgaccggtcg aaaagccgct caagcagcca
    45301 ggcatctatc tagtcaaatg cgatgcacac aaatttatgc gcgccactgt gctcgccttc
    45361 acgcacccct tttttagtgt cacagacgac accggcgcgt ttcagattgc ccaactcacc
    45421 ccgggagagc acgttgtgag ggtatggcac gacacattag gaacgttcca acaacgagtc
    45481 acgattccaa cccgcggcga agccaccatc acgatcgaat accccgctca tgtcgatgcc
    45541 aataaaaaat aggactgact ggttctgccg cccctggcgc atctggcacg ggaacctccg
    45601 cctccgtctc agcgtcggaa tggcggtcct gattacgatc ggcatggccg gtttcgcgac
    45661 cttccggtta attgaaatcc gtcagtccat cgaacaggcc acacaagccc gggcgctggc
    45721 ggtcagccgc acgttttcaa tggtgggcgc cgccgccgtg ttggacaatc tctttcgcat
    45781 ccaagaggcg ttaggtcgtt atgcccagga ttcggagatt ctgagcatcc tcatcatcga
    45841 tcccgacaac atgatcgtcg cagcgacgaa tcctcagcaa atcggacagc agctgacgga
    45901 tgagaccctc gcgcaggcac acgaaaccaa cgccgagacc atttcccatg gccagactat
    45961 gggcggcgtg ccgacactcc tagtggccac gccgctcctg aacgagcagg acatcgccac
    46021 ctgggtgcgg atcgagtttt ccctcgcggc gatgcaggag gaactcaccc gcgaagcgcg
    46081 ccgcctcttc ctcctgtcag tcttattcat gggggccacg atttcggtcg ctcaactcgg
    46141 gatcaggcgc atgtccggcc tgtttcgaga aactgccaaa aagctgcagg agaccctcca
    46201 taccctccat tgcgtctcgg aacaggcgcc ctccggcgat gagacatccg ataacggcaa
    46261 ctcccctgcc tcaccgtcct gcaagggcga actggagcaa gtagtcgatc tggtcgagac
    46321 gaccaccacc ctgctcacca cgcaggcaca acggctccaa tccttcaccg actcactgga
    46381 gcaggctgta cgggaacgaa ccatcgaatt gcatcaggca aaggaggcgg ccgaggcggc
    46441 gaatcgtgcc aaatcgcaat tcctcgcgaa tatgagccac gaaatccgca ccccgatgaa
    46501 cggcgtactg gggatgacgg aactcctcct cactacaacg ctcacgccga agcaacgttc
    46561 actagctgac accctgcacc ggtccggcac cgggctcctg gacatcatca atcagattct
    46621 tgatttctcg aagatcgagg ccggcaaact gaagttggaa atgatcgagt tcggcctcag
    46681 acagacggtc gaagatgcgg tggatctgtt tgcagaaacc gccggtcgca aacacctcga
    46741 actcacctgt ttcgtgccgc cgaacatccc ggacaccgtg atcggagatc ctgttcggct
    46801 ccggcaaatc cttctgaatc tcgtcggcaa cgccatgaag ttcacccaca ccggcgacgt
    46861 ggccgtcacg ctcgaactca tcacccacac cacgacgaac ctgacgctga aattccgcgt
    46921 ccgtgatacg ggagtcggca ttcccgaagc agcacaagca cggctgtttc aggcgttctc
    46981 gcaagccgat aactccacca cacggaaatt cggtggcacg gggcttggcc tcgcgattgt
    47041 gaaacaactc gccctcctga tgggaggcga cgtcggcgtc gacagcgtgt ccggccaggg
    47101 atccaccttc tggttcaccg tcgaactgga acgcgccgcc cagcgtacgg ccgcctcggg
    47161 agcaaccgaa agaccgttag acggattctc catcttcatc gtcgatgaca attcgacgaa
    47221 tcggcaaatc cttgaaacgc acttgaccgc ctggggcgcc gcagtgctgt ccgccacctc
    47281 cggcctggaa gcgttggcgt tgctcgatcg ccaatgcacc gcccggcgca gaatccatct
    47341 ggccgtgctg gacattcaca tgccggacat ggacggcatt acgctggctc ggaccattag
    47401 agaagatgcg cggctttccg gcatggggct cgtcgcgctc agttcggtcg accaggtcac
    47461 agacgaatcc cagcggtacc cgtcgctctt cagtgcctgg ctgagaaagc cggtccacca
    47521 gtcgttgttg aaagattgcc tcacacgcat gtgccaccac gtcccggcga ccgaggcacc
    47581 cgcgccgacc cagcccgacg atcacgccgt cccattcgcc gcccacgtcc tgctcgcgga
    47641 agacaacccc gtcaaccgcg aagtggcatt cacgatgctg gagctacttg ggtgcaccac
    47701 caccatggtt gaaaacggcc gggaggcggt cgaggcgctc acgaatcggc cattcgacct
    47761 ggtgctgatg gattgccaca tgccggaaat ggacggcctg accgccaccg ccctgattcg
    47821 tgaacaagaa acgcaggccg cgcagccccg gcacacgacc atcgtggccc tcaccgcaaa
    47881 tgcattggaa ggagaccgcg agcggtgcct cgcggcaggc atggatgatt atctgtccaa
    47941 gccgtttaca ttgaccacgc tcaaggaagt gctgcaccgc cggctttcga tcaaggggcc
    48001 ctcacgaaca accgaacccc ttctgcggtc gacaccgcag gcaccgcccc cacagacggc
    48061 accaccgacc ggacccgccg cgccaggcga cgaccatgtg gacctcaatg cgtggcaggc
    48121 gatccgtgct cgccaacgac ccggccagcc ggatcttctc cacaannnnn nnnnnnnnnn
    48181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48241 nnnnnnnnnn nnnnnnnnnn nnnnncctga tgcgtcaaga actcaaccgg gcaggacggt
    48301 cggccgcatg atgcacagtc tgaaggacaa gccgtgacga tgcgtcggtt cctgcaacga
    48361 ctccggcaga gcgtgcggcg cacaagcggg caactcgcga ccgactcggg cgactcgcaa
    48421 accgaacacc gggagattcc gccttccaac acgcctccgt ccctcgacga aggccgcgca
    48481 ctcgacgccg tcctctgctc gctcgaagaa ggcctgtgca tcgtcgattc gcaatggcac
    48541 atcctcagac tgaatcccca ggccgagatc ttaatcggcg cccccgccca tggcctgaaa
    48601 ggacgccccg cgtatcaact gattgctccc ggcccggagg aattccgcca cgagtgcctc
    48661 gtaaccgacc gctccattcc tccgctgcaa tccggactcc cctaccggac cgacaatggt
    48721 ctcctgctca gacaggatgg ccagttgacg ccaatttctc tgacgatcac tccgatgtcg
    48781 catgatggcg tcgtacgcgg agccgtcctg acgttccggg atctcacagc ccagaaggag
    48841 gcggagtcac atcagatcga aaactccgcg ttactccgcc gcttacagac aggcatggtc
    48901 aggttggcga gcaatcaggc gatctaccgt ggacaattga ccgaggcctt ccacctcatc
    48961 acccgggtca cgacacaaag cctccgcgtc gcgcgggtca gcatctggtt ttatgcgccg
    49021 gaccatgcgg tcctccggtg cgcggacctc tatgaccaac cgggcgatca acactcacac
    49081 ggcatcgagc aacccgcggc cctttatcca cagtacttct catcgctcga ggcgggagag
    49141 atgatcgtcg cgaacgaggc gcaggcggac ccgcgaacgg tggaatttgc agcacaccat
    49201 ctgctgccgc tggggatcac ctccatgctg aacgtgccta tccgcgccga gggcattctc
    49261 gttggggcgc tgcactgtga acacatcgga acgcccagag tctggacgac ggaggaacag
    49321 cagtttgcgg cctcagtggc gaacaccgtc tcgctcgccg tagaggcagc ggaccggcat
    49381 aaggctgaaa ccgaaacgaa acgtgccgaa caatttctcg actcggtgat cgaaaacctc
    49441 cccatcatgg tctatgtgaa agaagccgaa cagctccggt tcacgcgctg gaatcgggcg
    49501 gcggaggagc tcacaggatt cagtcgcgaa ctcatcctcg gccggaccgg ccatcatttt
    49561 ttccgcaaag aagaggccga ccgcttcaca cgtcatgatc gcgaagcgct ccggagcggc
    49621 cgcctcatcg aggtcgcttc agaagaaatt cacacgcagc accggggcgt acgactgctg
    49681 cgcaccaaga aactgcctgt catggatgag caggggcgcc cccgcttcct gctcggcatc
    49741 gccgaagata tcaccgaccg gaccgcgcac gaatcgagtc tgcacgagag cgaagagaag
    49801 taccgcgagc tgttcgagtc ctcgcgcgat gccatcatga ttttgtctgc gccgaattgg
    49861 aatttcacgg cctgcaaccc ggcgaccgtc cagctgttcg gggcacggga cgccgagcat
    49921 ttcacgtcac tcggaccgtg ggacgtctcc cccgagcatc agcctgacgg cgaagaatcg
    49981 atggtcaagg cgccgaagat gattgggata gctctgcagg aaggctctca caacttcgag
    50041 tggttgcaca aacgagtcgg cggtcccagc ttcctggcga gcgtgcagtt gacccgcatc
    50101 acgttgaaag ggccccccgg actgcaggcg accgtccgcg acattaccga acaacgccgc
    50161 agcgaagccg cactgcaggc ctattcgaca ttccagaagg ccatgctgga caacgccggt
    50221 catgcgatca tctcctgcaa acccgacggc atgatccaag tcttcaatcc ggcggcggaa
    50281 gccctgctgg gatacagcgc cgacgagttg gtcggcaaac agaccccggg catcttccac
    50341 gacttggacg aagtggtcgc gcgagcacgt caattttcca aggaattagg cagcacgatc
    50401 gaaccaggct tcgacgtgtt tgtcgaaaaa tgccggagga atctccccaa tgagcacgag
    50461 tgggcgtacg tgtgtaagga tggcacccgg aagacggtgc tgttgaatgt cacggcactg
    50521 cgggaggcgg atggccggat caccggctac ctcggcatcg cctccgatat cacgccgctt
    50581 aagttggcgg cacaggagtt ggtttcggcc aaggaagcgg ccgaggcggc caatgtggca
    50641 aagtcgaggt tcctggccaa catgagccac gaaatccgca cgccactgaa cggcgtcctc
    50701 ggaatgaccg aactgctgct gagcaccagc ctgtcggcca aacaacagtc gctgctggaa
    50761 accgtccgcc gttcgggggt ggccctactc gaagtcatca atgacattct ggatttctca
    50821 aagatcgaag cgggaaagct tgaactcgaa caggtggagt tcggcctgag acaggtcgtc
    50881 gaggaggcag tcgagatatt tgccgggtcc gccggcaaca agaaactgga gttgacctgc
    50941 ttcatccctg ccgacgtgcc ggatagcatc gtgggagacc ctgtacgcct gcggcaaatc
    51001 ctgctcaacc tgatcggcaa cgccatcaag ttcacgacac agggagaggt ggcggttgcg
    51061 tttgcgctga cgaaacaaca tgcggatacg ttcacactgg cgtgccaggt acgcgatacc
    51121 ggcatcggca ttcctgacgc cgctcaggcc caactgttca cggcgttcac ccaggccgac
    51181 ggctctacca cccggcgatt cggcggcacc gggctcggcc tggcgatcgt caaacaactg
    51241 gtacagctga tgggtggtga agtcggcctg gtgagtgcgc ccggccaggg cacgacgttc
    51301 tcgttcacat tcaccgtcac acgtggaagt gctcacgccg cataccctcc gcttgccgaa
    51361 cgccatctcg ccggacggca gatcctgatc gtcgatgacg cacgcacaaa cctcgagatt
    51421 ctggccaccc acctgcggag ttggggagcc acggtcttca ccgcggagtc tgccgacgat
    51481 gcgctcctcc aattggagca cttccggcac acgcagcatc gaatcgacat ggccatcctc
    51541 gaccttcgaa tgccggggcg ggacggcgtc gatctcgcgc gttccatcaa acagcagacg
    51601 gcttatcaga acctccccct gatcgcactc agctcagtgg agcgtctctc ggacgacagc
    51661 gacgtaaccc accggctctt tcatagcttt ctgcgcaagc cggtgcgaca gtcactgctc
    51721 aaaggctgcc tgacgcaggt gctcggaggc tccgccggaa cagtgacgcc aagtccgcaa
    51781 tcctcagcgg ccccgcgacc acgcgtcgaa gcccacatcc tcctggccga ggataacccc
    51841 gtcaaccagg atgtggcgtc ctccatgctg gagatgctgg gatgtcgcat cacgatcgca
    51901 cccaacggac gggccgccgt cgaggcagcc acaaccggcc ggttcgacct cattctcatg
    51961 gattgtcaga tgcccgagat ggatgggttc actgccacag cagccattcg ccggcaagaa
    52021 gcgacagcga gcgaccgccg ccatgtgccc gtcattgcgt tgaccgccaa tgccatggaa
    52081 ggcgatcgcg cccgttgcct ggccgccggg atggacgact atctcaccaa accgtttacc
    52141 gtcgcgcagc tacacgccgt cctcacgcaa tggttgacgc cacaaccagc caaggcaagc
    52201 gagaccgacc aggcctcctc cccccatacc accgacgccg acgaaccggc cgccacagag
    52261 gaagcaccgg tttcgacaac catcgacaag actgcctggg atgcgattct gtcgctccaa
    52321 cgcccgggac ggcccgacat cttagcccgc gtgctggcga cttacttgga cgattcccgc
    52381 ctgctggtgg agcagattcg ctccgccgtg cagtcacagg atgctgtcgt tctttgccag
    52441 gccgcccacc gcttgaagtc cagcagcgcc cagctcggcg tcctggccac cgccgcccnn
    52501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ccaccgcttg aagtccagca
    52621 gcgcccagct cggcgtcctg gccaccgccg cccattgcaa agaattggag acccttggac
    52681 ggcttgccag gatcgacgag gctgcgcacc ttctgtcgca actcatcgaa gcccatcagt
    52741 tcgcctgcgc cgcgatcacc tcggaactac aacagcgctc cgcccgataa accaaccgct
    52801 cgaccggctc tcgcgacttg cgaatgaacg tcgtccgcgc tagtctgcac gcactcatgg
    52861 ctacctatgc aatcggcgat gtacaaggct gctttgcctc gctcagacag ctcaccaaga
    52921 cggtcggatt caatccggca caggaccgtc tctggttcgt gggggatctc gtcaatcgcg
    52981 ggccggattc cctcgccgtt ctgcgttata ttcgtgggct cggaccggca gcggtcaccg
    53041 tactcggtaa tcacgacctg ttcctgcttg ccgttgcgac cggcctggcg acccttcgcc
    53101 gcgacgatac gctgacccaa gtcctggacg ccccggactg cgacgaactc acgacctggc
    53161 tcagacagca accgttgctc tatcgggagg gttcttatgt actggtccat gccgggctct
    53221 tgccgcactg gacaattgag gaggcccagc aattggccat ggaggccgaa acagcactgc
    53281 gtggagaaca attcagcgca acgctccggg ccctccaccc cagcggccat ttacaatggg
    53341 cgcccgatct gaaaggcccg gtgcgccttg ccaccatcat caaagtgttg acccgaatca
    53401 gaacctgctc cgacgacggt atcatggaat cgaccttctc cggcccgccg aaactcacac
    53461 cccagggatt tcacccctgg ttcgatctcc cgaaccgacg aagtgcgact gccacgattg
    53521 tgttcggaca ttgggcggcg atgggcctgc atctcacacc gaatctgtgt gccctggaca
    53581 gcggctgcgt ctatgggcgg cagctcaccg ccgtgcggct ggaggaccgg aaggtcttcc
    53641 aagtgcgttg cgaggagaca cgtggaggta aatgagcccc cgctcacggt cgattggtcc
    53701 cgctacagta gcagggcgct gccgacctac cgattcctcc cgggcctgac tccacatcca
    53761 cgccgcgatc cactggggca ttccttcgca gaaccggagc cacgcccgcg cccttttgca
    53821 cagcaccaat ggccagattc agaagactat ttgtatgcgg tcgatctcta taacttcggg
    53881 tactggtggg aatcgcacga agtgttggag gggttgtggc acgcgtgcgg acgcagaacg
    53941 acggcgggca actatttcca agccctcatt caattcgccg ccgcccatct caaacgcgcg
    54001 ctcggcaatg aggtagccgc gcacaacctt gcccatagcg ggctcctgcg cctgcagaac
    54061 agtcccgcct cctacatggg gcttgatacc gtcacattcg cccatgacat cacatcgtgg
    54121 atgggggacc acagccggcc agccgtgcgt attcgtttgg aaagcttcgg caacgggcca
    54181 tcagtgaacg aggccccctg agcaatccat cgctcctggt agggtcagga acaccttcga
    54241 tggttcaagc caggtccgcc gacaagtccg aggtgagaag taaacagcat cgtcgctgct
    54301 ggcaacggta aggattgatt ccagtcggga tggaagttgg ggggaacggc aacgggctag
    54361 tgataacttt cagaatcgga cgggaaggtg ccctgctcaa cctcatcctt gtaccggcgg
    54421 agagcctgca gagcatcgac cttgagatgc gcgtagggtt tgacgaactt cggcacaaac
    54481 tcatcgaata gacccagcag gtcatacagc accaacacct gaccgtcgca atgcgggccg
    54541 gccccgatgc cgatcgtcgg aatcgtgagt gcctccgtca cggtcttcgc caaccccgcg
    54601 ggaatcgctt ccagcacgat ccctacggcc ccggcagctt ccaacgcctg cgcgtcttca
    54661 agcagttgct gcgcccgatc cttgcccttc ccctgaacct tatacccgcc gtactgatgg
    54721 actgattggg gcgtcatccc gaggtgcccc atcaccggga caccaatttt cacgatcgcc
    54781 gacacacggt ccgcaaccgc agctccccct tccagcttca cggcatgggc tccggcctga
    54841 atgaaccggc cggcgtttcg aagcgcctcc tcctggctga cctggtaaga cagaaagggc
    54901 atatcgccga tgaccaacga ccgctgagcg gctccggcta caagcttcgt gtggtacagc
    54961 atctcctcca tcgtgacgga gagggtattc gacttgccct gtaccaccac ccccagggaa
    55021 tcgcccacca ggatcacgtc aatcccggcc tgctcgacca tgcgcgcaaa caacgcgtcg
    55081 tacgcggtga caaccgcgat cttccgcctg tcgcgcttgc acttctgcaa gtcgggaacg
    55141 gtcattaccc cagctctgct ttcgtgagaa tttccggcag ccgttccgtc gacttgatcg
    55201 ccatgatgtg cactccgtcg cagtacggcc gcacagcttt gatggtgcgc acggcaatct
    55261 cgacaccgac atcgagtgcc cgcttgtccc ccgccgcgcg catttcgtcg atcatgtcct
    55321 gcggcacaca gacgccggga atgttggcgt tcatgaattc tgccatcttc gcgctacgga
    55381 gcaacagaat tccggccagc accttgacct tgaatggacg caccgcctcc atgaatttct
    55441 tgaactgctc cggctgataa atcgcctggg tttgaaagaa ctccgcgccc gcccgcactt
    55501 tgacctcgaa cttgaccaac atcgggccga gcggatcggc ttcgggagtc acggccccgc
    55561 cgatcgtaaa agcggtggac ccgtcgagct tgttgcccgc atagtccttc ccgttattca
    55621 atcccgtgac cagctgcatg acttgaacag aatctaggtc gtacaccggc ttggcttctt
    55681 tatggtcgcc tacggtggga tagtctccgg tcagacagag gatgttccgg atgcccagaa
    55741 tgtgcgcccc catgagatcg gattgcatcg cgatccgatt acggtcccga caggtcagct
    55801 gcatcaccgg gtcatgcccc aactcataca acacacggca gaccggcaag gacccggcac
    55861 gcacgatcgc ggccgtgttg tcggtgacgt tcactccatg cacacgtccg acgagcgcct
    55921 tggcactctc gaccacatgg gtcaggttcg tgcccttcgg cgggttatat tccaccgtca
    55981 cggcaaattg tccctgggcc aatacgtctt tgagtcgccg cggctctcgg ctcatgcgag
    56041 tctcctatac atctcgatcc ttacctgccc ttctccactg tcgcaacgga caagccgctg
    56101 gcccaacgag ggccttccgg ggccgcgcgt gatgcgatgc gagggatgcc gggcatcacg
    56161 tgggcgctca ccggcgagca aatgagccgg cagcctctcg atcaacccta tttcatccct
    56221 gccattctct tagcagattc aaccgtattg tcgagcaaca tcgcaatcgt catcggtccc
    56281 actccgccgg gaaccgggct gatccaccca gccttctggc tgaccggctc gaaatccacg
    56341 tcgccgcaga gcttgccgtc cggcagcttg ttggtgccca cgtcgatcac gaccgcaccc
    56401 tctcgcacca tatccgccgt cacgaacttg gctttcccga tcgccaccaa cagcagatcg
    56461 gcctcacggc agaccgccgc aaggtccttc gtcttcgaat ggcaaatcgt gacggtggca
    56521 ttgcgttgga gcaacatcag cgccaacggt tttccgacga tattgctccg tccgacgacc
    56581 acggcgcgct tgccttcgat cgacacgccg gtcgattcga tcatcttgat gacccccttc
    56641 ggcgtacagg cttcgaagac cgggttgcct tcgactaacc gtccgaagtt gtaggggtga
    56701 aacccgtcgg catccttatt cggcgagacc gcctccagta ccacgcggct ctcgatgtgc
    56761 ttgggcagcg gcagctggac caagatgccg tgaatcttcg gatcggcgtt cttcttttcg
    56821 atcaatgcca gcagctctgc ttgcgtcgtg ctagccggca acttgtgatc gtccacatag
    56881 atccctgcca attcgcaggc cttctgttta ttcctgacgt acacgtgcga cgcaggatcg
    56941 tctcccacca aaatcgtcgc cagtcccggc ttgactcctg ttttggccag caccgcggcc
    57001 gactctacag ccagtcgttc ccggacttgc tgtgccaacg cttttccatc gataattcga
    57061 gctgccacga tatcctccct ggcctgaata gaatcaaaaa gtgtcggcac catagcaggc
    57121 aatttttcaa caagtcaacg ctggcggctc cgcgtagacc accgacaacg ccccgttagg
    57181 aagcgatatt ccaccgctcc tctcctgtcg cagccacttc gcaatcagtc gcgcctccat
    57241 gcaccgacct gcggcttcct tgacagtctc cgaggccctt ggctacgatg catctacaga
    57301 atcctctgtg cgacaccttc gttcatgaat tccaggacca tccgccacaa cagattcaca
    57361 cggctactgc gccgccggag cttcggcctg gcgctcgctc tggccattgg agtcctgttc
    57421 catcaaccct gggcctgggc tcttcaagtg agcggactgg agtctccgca tagttttctg
    57481 gccgacccgg cgacaaggtc ctattacatt tccaacatca acggggaagc cgatgcccgg
    57541 gataacaatg gattcattac caagctgagc gacgacggcc ggatcaccgc cttcaagttc
    57601 attgagggcg gccgcggaga cgtcacgctc catgccccca aaggcatggt ggtcgtcgat
    57661 gacgtcctct atgtcaccga tctggatacc ctgcgcgcct tcgacaagac gacgggtaaa
    57721 cctctcgcca ctctggtgct cccacgcccc gcagccggcg gcgccgccca gtccctcacc
    57781 gatgtggcct ccgatggcca aggccacctc tacctggccg accagggcgg caacgcgatc
    57841 taccgcgtgg atctagtgcc gacattgacg ttgtcgttgt atgtctccgg tgcgcacttg
    57901 gccggtccct ccggagtcgc cgtgcatccc aagaccggac atgtggtggt ggtgagttat
    57961 gattccggaa agattttcga catcaccccg gaaggcacgc tcacagaact ggcatcgaac
    58021 ggatttttta ccgcccgatt ccagaatctc agcggcgtgg atttcgaccg gtggggcagc
    58081 atgtatgtct cggatgggac gaagggcaag atctggcgca tgctcccgaa tggaaagttt
    58141 caagtgatcg cggagtacct gccgggtcca tccgatgtgg gaatcgaccg agtcaaccac
    58201 ctgattctcg tcccctacca agactccaat gccgccgagg tcaacggcct cgaatcgccg
    58261 acggcttcat cgggtgatcg ggccaaacgc acactggcgg actatggatt cgtcgaaccc
    58321 aagaaatccg acaaggaagg atctcctcgc aaatgagccg atgtgtctac tgcaagcaac
    58381 gcaagggcaa acgttcctgt cccgccctca ccggtttgat ctgcagccaa tgctgcgggg
    58441 aacatcgact gacgagaatc tcctgtcctc ctgactgcga ctatctcgac accgggagcg
    58501 actaccagca gaagcggctg ggcgagcagt ttgccccggt ccggcgggag ctctaccggc
    58561 aactgagtgt ggcgggaggc gaaaaggcgg ccgcgctgtt caacctcatc gaggtcgtca
    58621 ccttcggcta tttccacgac cggcgggacg gccaggatgc cgaagtcttt gccgccatcc
    58681 aagcccttcg tcggaccttg agcccccttc atgtgccgtc ggccccgatg ccggtgtttg
    58741 cggaacgact caagaaggaa tacgacacgt tcgtcaaaca acagcctcaa caggtgagtg
    58801 atgctggttc agccccggaa attctcgatc gcgcggtcgc gatcctcaca gagttttcag
    58861 gccaggactt tcaatcacgc cgcttcctga acggcatgat cggctacgtc aaaaccttcc
    58921 accccgagat tgcagagcat ctgaccaaac agcacgaagc cggccggatc gtgctcccgg
    58981 gccaactgac cccgcccccc caggagccga cccatgttca cggcccagag tgccatcacc
    59041 accaccacta aacccgcatc tcgtcgggcc ggcatccgtc actgccccgc catgctcgtc
    59101 accacccgct cctattaatt cctgtacctc cctacgctca ttttctcttt agtttcggct
    59161 gatttgtacc gatacaggcg tgccaagacc tacgactgcg cgaaagagcg cggccaggcg
    59221 aatcgccaca actaactgaa actcatgggc tctttcgtta tggatctgct ggcaagtctg
    59281 ccgaagggag atgcactggc cgaaccggca tggaactccc gtcacagggg aattctttcg
    59341 atactctggc tccacgtgct gagcgtgccg atgttcggta tctacatggg ggcaagtccc
    59401 tccctctatc tcgggggagg agggctcctt gccttcatcg ccgtcgccgc acggctttcc
    59461 gttgtccgcc gccgtctcca atctgccatc gccacctgcg ggctcatgac agcttcggcc
    59521 ctcctggtac acctctccgg gggtcaaatc gaactgcact ttcatttttt tgtgatgatg
    59581 tccgtcatcg tgctgtacca ggattggctt ccgtttctcg tcgggctcca atttatcatc
    59641 ctcgatcacg gagtggtggg cacgctcatg cccgacatgg tgtacggaca tgcccaaggg
    59701 caaacacacc cctggacctg ggccttgatc catggcggtt tcatcctggc cgaatgtgcg
    59761 gccctgctct acttctggcg ggtcaatgaa ctcgcacaaa atgaggtccg gcaaagcgag
    59821 gccagaactc gcatgatcat cgaaaccgcc ctcgacgccg tgatcacgtc ggatgccacg
    59881 gggaccataa ccgggtggaa cacccaggct gagttgatgt ttgacgtccc acggaccgag
    59941 gcgatcggaa aatacctgac cacctacgta tccgcctcat acccctcaac tgaaccaggc
    60001 cccatcactc cgtacgcggg gttagtcccc ggagccatcc tcaaccggcg tagtgaaatg
    60061 ctgggccaca cctgccaggg aacaagtttc cccgtggaaa tcgccatgag ctgtctgacc
    60121 gtcggcggga cgcagcagtt caccattttc gtgcaggaca tttccgagcg aaagcaacag
    60181 gaagagcgcc tccatcgcgc gaaggaggcc gccgaggctg ccagcctggc caaatcccaa
    60241 ttcctggcca acatgagtca tgaaattcga acccccatga acggcgtcct cggcatgacc
    60301 gaactcctcc tgacgacccc gctgaacgac aaacaacgcc gatacgccga caccgtacac
    60361 aactcgggaa ccagcctgtt gcacatcatc aatgacattc tggatttctc caagatcgaa
    60421 gccggccgcc tagtcttgga atacatcccc ttcggcctcc gtcagatgct caccgaaacc
    60481 atcg
//
LOCUS       DAOQRM010000002        50600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_309_length_50600_cov_18.879652, whole genome shotgun sequence.
ACCESSION   DAOQRM010000002 DAOQRM010000000
VERSION     DAOQRM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 50600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50600
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_309_length_50600_cov_18.879652"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..865)
                     /locus_tag="PKK81_00255"
     CDS             complement(161..865)
                     /locus_tag="PKK81_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNV31325.1"
                     /translation="MEKEVGLFMNQQPTLVYILGRFLVAGSILLAGYAQYHVLRPTSV
                     HAAEFADVYLSLAVVGGFPEDRGLNLGGQGAPKVTLYNSLGAGVRIGIFPEFTRRIVG
                     VEIEYFGTTGRLLAATSGNNGRGEATAGLTVTNSMTNLIARKPDGEFRPYAGVGVGYS
                     SAIFHGASFPGRTNQDFDSTAAFSYQLMVGVQYSLGGRTFLFSEYKRVTANFHWSDVS
                     LDYHAHHALAGIGWIF"
     gene            complement(1020..1364)
                     /locus_tag="PKK81_00260"
     CDS             complement(1020..1364)
                     /locus_tag="PKK81_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNV31326.1"
                     /translation="MKRMLMSLGVVAFAASLYGCSGVAGYTQPAGIFPTAGIYMETTS
                     GGILHDNGATATKTGKSCGTGLLGIVATGDTSVEAAMNAGGIKKAVFVNHYIKSFVWG
                     LYVETCTIAKGN"
     gene            complement(1555..2040)
                     /locus_tag="PKK81_00265"
     CDS             complement(1555..2040)
                     /locus_tag="PKK81_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNV31327.1"
                     /translation="MRIGVVSDTHGLFDRALLRHFSGVAHILHAGDIGKRGVIEQLTQ
                     IAPVTAVSGNVDGFEASGFPIEQVLELAGHRIALYHRLYEGGRLTREGTGFLTRTHPA
                     MCVYGHTHQPKVEWQEGVLLFNPGSAGPKRFHLPRAVGLLLLDVGTIDARHIMLADRA
                     E"
     gene            complement(2055..3188)
                     /locus_tag="PKK81_00270"
     CDS             complement(2055..3188)
                     /locus_tag="PKK81_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV31328.1"
                     /translation="MNPTPNALCFGDGLPGAGAPCYVTVSADGLTMAPVGNSLLVEEA
                     IAFTAMSVEAGGLDHDQLVASWGVGASVRTLYLKDPALIIAFRRAAPSALMAHLERAA
                     GQVRRARHSHRALWGSAVGVVVGLGLMLWFGSDLIVEWAVARIPVQWEQKLGETVYQD
                     FLSKETVLKEGPAVSAVQEMTQRMTAKIPNNPYTFQVSVVQSSVVNAFALPGGYVVVF
                     TGLMKKAESGEEVAGVLSHELNHVLQRHGMERLVKMMGLAAVVSILTGDQQGLIGLAR
                     QLGLELATLKFGREQETEADVTGIRLLHDARIAPDGMIRFFERLSEKDKERVELFSSH
                     PMSAARAERLKAELAALPKQTTEPFTFEWKGVQDSLGASEPKK"
     gene            complement(3326..4009)
                     /locus_tag="PKK81_00275"
     CDS             complement(3326..4009)
                     /locus_tag="PKK81_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNV31329.1"
                     /translation="MKSEILYPGAFPMVRVYLNEGETVKAESGAMVAASPTIDIESKM
                     EGGFLGALSRKMLSGEKFFFQTLRASRGAGEVLLAPTVPGEIVLLDLDGVNEYMVQKD
                     GFLAGAEGVKIESKMQSLTRGLLGGEGFFILKIGGTGQLILNSFGAIHKIELKPNEEY
                     IVDNSHLVAWTSTTTYNIEKASSGWIASLTSGEGLVCRFRGPGVVYIQSRNPGSFGNW
                     IRQFIPVSE"
     gene            4268..4603
                     /locus_tag="PKK81_00280"
     CDS             4268..4603
                     /locus_tag="PKK81_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31330.1"
                     /translation="MKRVVIEELTTGMILAKPVTNNAGLVVLPVGAELDEATLSRLQR
                     LGLTSVYVEGEAGDASGKTLDELEAELDHRFRLVAQDPIQCLLREAIRLHLRTTHGIS
                     DNPEATPSA"
     gene            4600..5436
                     /locus_tag="PKK81_00285"
     CDS             4600..5436
                     /locus_tag="PKK81_00285"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV31331.1"
                     /translation="MNPSRKIDLHRRIEQVGELPTLPHVVQKLASMIGRPNVSAEEIG
                     TLLEKDQVLSAKVLRLANSPFYGFPSRIASVAHAVVVLGLNVVKGLTLCATAFDMMRN
                     AGMNELWRHSLGVAMTAHILGTKAAMKNPEEVFVAGLLHDIGKVVLYVKWPDVGQQIT
                     TVTRNTSRPLMDTEQELFEVTHADVGGWLASAWHLPTSLREPILHHHTPAAAQDAKLQ
                     TAIVHVADVLVKGLACGNPGDDLVPPLSRQAWDLVGLDAQSLAHCLTQATEEFQTIDD
                     YL"
     gene            5433..6407
                     /locus_tag="PKK81_00290"
     CDS             5433..6407
                     /locus_tag="PKK81_00290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV31332.1"
                     /translation="MSGSPSNRRILLLHSDPLEIERLTTGLNRSGFTVVAADAGRLAM
                     HELVHDPPCLVLAAEGTNGRSADTLARDLRADPFLGRLPLIILVRDIRVNDLDWATLG
                     VDDYIAVPYRPDEVPQRVRLCLSRLERSLDANPLTRLPGNSTILHETTARIESRAPFA
                     LAYLDIDNFKSFNDRYGYARGDEVLVVTCRILTTVVSELTGTDGFVGHVGGDDFVFMS
                     APSAIDAICQTLIKRFDLVIPDFYDPEDRQAGFIDSVDRRGNHERFPIMSLSIAVVTN
                     EHRDISHPGDVSKIASELKKVAKSRPGSVYVKDNRTNPPDSAPESTPR"
     gene            complement(6459..6965)
                     /locus_tag="PKK81_00295"
     CDS             complement(6459..6965)
                     /locus_tag="PKK81_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNV31333.1"
                     /translation="MRALLVCVIVLACGVASAGAQGDSAGVPWSQLSPGEQQLLQRFS
                     DNWDQLPPRKQQRLRNGAQQWGTMTPEERQEAKQRFKQWRTLPPEQQQQLRERYQQFR
                     QLPPEQRESVRDARRWFRSLPPEQRKELREKFKHMSPEERRAYRRELRRQYGGNAGGQ
                     APGGSSAP"
     gene            complement(7266..7625)
                     /locus_tag="PKK81_00300"
     CDS             complement(7266..7625)
                     /locus_tag="PKK81_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31334.1"
                     /translation="MNERSDEQRDPLAVAAKHLLDRSVQDLDQKTVLSVQRARLAALD
                     AASRRRQPWLRWAGGVALASMATLMVTIWLWQSNGANHSHLLLEDLELMQSPENIELS
                     EDLEFYNWLADGTATTG"
     gene            complement(7622..8239)
                     /locus_tag="PKK81_00305"
     CDS             complement(7622..8239)
                     /locus_tag="PKK81_00305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV31335.1"
                     /translation="MNGCHSQIAVDGQPGSLCLGERVGSLDRTQALDRFLAGIERRAF
                     RMAHIATGNKDEALDLVQDAMLKLAHKYGERAEEEWGPLFHCILQSRIRDWYRRERVR
                     NRLREFFRSPHDEEEGEDPLEQIADSTAVAPDEDVQRKRACAELDVALRALPLRQQQA
                     FLLRIWEELDVAQTAQAMGCSQGSVKTHLFRALQVLRTRLGAHWP"
     gene            complement(8619..13502)
                     /locus_tag="PKK81_00310"
     CDS             complement(8619..13502)
                     /locus_tag="PKK81_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31336.1"
                     /translation="MRSDKKSPLKSDKKVPPKVQKKGSIGFNKKSALLEDAITQMNAG
                     KYGRASSALKDLLALDPLNAEARRLFATLHLRLGSLMSARTAFESLAREAMERQDYWL
                     AESLLREYLTAGPRYVPFLEMLGRVYEEKGDVMAAVAEYGKAVEVLLEDPDTDHPNRA
                     SDLFVRIRSLAPGSPVAFRFAAMFDTVTGQVLQVTPQPPVVDSINETAHAPALIETPP
                     VTDSAVVMPWEQMETASSDVPRQQEIQVSAVEQSPEVIAFTTTSSTLEAVTEDLLQAK
                     PEESLSVTLPVDSPQESEVQPQSESSVSAVTSAVESVQAAGESDPPVLEVDPASIEPA
                     EIASASVAEVSTPTMDASAKTGPDSVATLSAHSAPMPWDQVEEAAGIIPPQPEGPAAT
                     PADIAPPPSGEVGLTPSVAESPAPSIEVLTQSSGHPIATAPASPAPMPWDQIEEVAVV
                     IPSPPSSPEPVIVDAESLPPTQSEVSTLPLAELAPFQPVEPEPPAAAAPMPFEEMDLQ
                     APVSEPIAPTIEVLTQASSHPIAQAPTSPAPMPWDQIEEAAGAVDPRTETLMEPGTDE
                     VEKAIESPAPNQAAEVSQAAESVSSSRPSELTSSGLSWEEILAAVAAMQTSPAPAQTL
                     SDGEQKPASDAAVQERTAGALTAPPAPPEEESPLFEATVPDIPADQAGAATTLSAPMP
                     WEQIEVDDVTIPRQEPEPEFGPAFAEGVVAVQDSTVALPAAPDVTEATVAGEACLAEP
                     VADRPVEPIATVTAELRILSPDAPTEAPEDSIRIPVEPYKAVEAPNPDTVPEAEQPAE
                     STIDRAVEAPLRLADSESVVATSLEEPPPASVEPVTEFAIAVNTDVTPMEPEQVTVEE
                     PQAPVGEAVAELPSEPVLPVEQPAEILCAQEVRVELAPEALAESRVPAAAPSLADEKP
                     VAPDAPLSPVVLGVTPQAEPEMALTDQEPSSPSAAEAREVDTVPISEPIVPPVEVSLN
                     PVAPSPEAASLPPSPLPIVVATEEPVLPPVEQTQAAVVSPSPELVEAPAESVAPSEEQ
                     APPPTAQVVSPPDAGEGLRILWDDTSSKPTPSASTGNMLTRWLKKPAEPVLAEVAEAT
                     PPATIPDEAPAPVPALSVEQQGPAVVAVERLPDEQETVTPMPVDAPVARPKPSQPVGA
                     GAWTRMGELVASLVGAGVSTTQSLVVMVLALVGLTLVLIGGAVGVVALTWLVLEEQPS
                     AAYRTMTSVPQRTLQDSSKNGYVLLLGFGAAASQDPVQAGMDRRVEGADRAYAHACLT
                     GEGVSSGGEQGASAELAGKWMKTADPAAQMRAEATGVKGWASRAEVSMGRYRQWLTKP
                     FEDWGYGQSITPNCGLILHAHRLYIAEGFAQDVEAGVTRLETDLTAWRTVLGQAKTLP
                     VKMLASAAMNDDIVVMSGLLLRPELDDRFVSRLAKLARPLEQAEQSVRWPMQSQFVLA
                     TKTLDEAVSEDRSDARPFYGSVAAALPLPKQRRFNAYAQYYEAAGKAAAEGRYTDLPK
                     QSQFVHAPPYGLGDIVMNPIESLVGVDPLPTWETYAGRVLETDARLRLASLQAWLRRT
                     PPEQDLLTRIAKAGQGVYDPFTGFPMLVNMKKGVLYSVGQDLKDNEAQDRFDLVAPIP
                     SVAWAGGKRPADADKSK"
     gene            13912..14742
                     /locus_tag="PKK81_00315"
     CDS             13912..14742
                     /locus_tag="PKK81_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNV31337.1"
                     /translation="MTKAHILILTDLDGTLLDATTYSYEAATGALSAIQTLGATLILV
                     SSKTRSEMEPLRLRLDNRHPFIVENGGALFIPMGYFPFPLEQAAPRGDYEVVEIGTPY
                     VRLRTALKEIGRDLGCRLRGFGDLSLEEVSDLTGLSPAETLLATQREYDEPVVVESNG
                     MVWNRLSAAAATRGLRWTRGGRFYHLMGGNDKGVASRRLIAWYERRAQQEGRALITVG
                     IGDSLNDLPMLEAVTYPILVRKPDGSYDSDVQFPHLTRAEGVGPVGWNRSLMDLLPTL
                     "
     gene            complement(14804..15025)
                     /locus_tag="PKK81_00320"
     CDS             complement(14804..15025)
                     /locus_tag="PKK81_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31338.1"
                     /translation="MTDKISDVDAEAKARRWLGGEAAVPRYPHRRQSPFVFNRSLVGL
                     VAFAVGLGFAGGLLAGLQCRRRPPVSAKE"
     gene            complement(15072..15779)
                     /locus_tag="PKK81_00325"
     CDS             complement(15072..15779)
                     /locus_tag="PKK81_00325"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31339.1"
                     /translation="MKEFFEKGLLDPLELMARQVLAVLPSLLAMSIIFFAGLVVAWTA
                     SQALERLLRVVGLDRLFDRLGVTGALLRGGVKTDPSRLVGQAAYWLVMIFATVAALGA
                     LNLQPINDFAKSFLAYVPHLVTAGLILVAGWLLSNFVSQAVLIAAVNAGLPPARLVAT
                     CSRWGIQLLAVAMALEQLGIAQNIVVVGFGITLGGLVMAGAIAFGLGAKDLAKDYLER
                     GLSIRTRDGAPDDLRHL"
     gene            complement(15837..17138)
                     /locus_tag="PKK81_00330"
     CDS             complement(15837..17138)
                     /locus_tag="PKK81_00330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV31340.1"
                     /translation="MTPGPQLPDATHTTPLTPETEAQVDQFAHTDILVGIPSFNNVET
                     IGHVVKAVSAGLAKYFPEARAVLVNSDGGSTDGTQQVVSQAVVDLKTLFIGDQQSSLH
                     KIITPYHGIPGKGSAFRTIFEIARRLKAKACAVVDSDLRSITPEWIELLVSPVFEQGF
                     DYVAPYYLRHKYDGTITNSIVYPLTRTLYGHRIRQPIGGDFGFSGQLAQHYLDKHVWE
                     SEVAKFGIDIWMTTEAIASGARVCQSFLGAKIHNPKDPAADLSAMLVQVLGAVFALME
                     DHYAVWGKTDGSNAVPLFGFQYEVGVEPVNVNVDRMISTFRQGLSDLGTIWDQILAPG
                     TRQSLRPLGTCSAQDFRIADNLWARVVYDAAVAYRNRVLPRDHVLKAFTPLYLGRTAS
                     FVLDTQGLTSSEAEGRIEALCQAFEKDKSYLVARWNEPHAG"
     gene            complement(17438..17803)
                     /locus_tag="PKK81_00335"
     CDS             complement(17438..17803)
                     /locus_tag="PKK81_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31341.1"
                     /translation="MEQQNRPRFIVECTGSRSEDGLLVWNGRMLDLSIPGWSRTGLKG
                     LQAGDLLQLHLHIPGQPRPLSVRLATIRWASESKLGVDPILMDADDQLRLNDFVSAHD
                     TTPPFSTDRKEQIVISDAG"
     gene            complement(18028..18372)
                     /locus_tag="PKK81_00340"
     CDS             complement(18028..18372)
                     /locus_tag="PKK81_00340"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV31342.1"
                     /translation="MAKSQTSRSHSRVPVSCFLYYLGEGLVGTGKVCDLSVKGWRIDG
                     DKPVTVGMKLTLRVFLPDQPKAIDVDGVTVQWVQGRVFGLETVRMSATAQARIHKFVE
                     SVLKESGSSRVA"
     gene            complement(18536..18910)
                     /locus_tag="PKK81_00345"
     CDS             complement(18536..18910)
                     /locus_tag="PKK81_00345"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV31343.1"
                     /translation="MTGGNKQVAPLAPRSSPAPVATTFQLRSFRRFPVQCSVYYSSDA
                     LQGTGVAWNLSLNGWRIDGTHPVEPGTVVTLCVFLPDQHPTVFIDRAVVRWSRGQEFG
                     IEVNEIKADEHARLEQFVTALV"
     gene            complement(18948..19024)
                     /locus_tag="PKK81_00350"
     tRNA            complement(18948..19024)
                     /locus_tag="PKK81_00350"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18988..18990),aa:His,seq:gtg)
     gene            complement(19193..19912)
                     /gene="pyrF"
                     /locus_tag="PKK81_00355"
     CDS             complement(19193..19912)
                     /gene="pyrF"
                     /locus_tag="PKK81_00355"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNV31344.1"
                     /translation="MMTRIDARDRLIVALDVPSAAEADALVARMGDQVRFVKVGLELY
                     TVAGPDIVRKLVDRGKRVFLDLKFLDIEETVRRATARVAAMGATFLTVHANRKALIAA
                     VQGRGSSDLKLLAVTVLTNFDGEDLREMGIQRSVQDLVTARAMLAAEVGCDGVVASGE
                     EPQAIRAKVGPNLVIVTPGVRPAGKGTDDHARVTTPTQTIAAGADYLVIGRPIRDAQD
                     PPAATAAILEEMQAAFDRRGR"
     gene            20026..20301
                     /gene="rpsT"
                     /locus_tag="PKK81_00360"
     CDS             20026..20301
                     /gene="rpsT"
                     /locus_tag="PKK81_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNV31345.1"
                     /translation="MPVVHKSTIRRARQSEKRRLRNRSTISSVRSILRKVQDAITAKK
                     PDEAKATLREATSALSKAVTKGVLKPNTASRRVSRLTVRVNAITASA"
     gene            complement(20285..21394)
                     /gene="holA"
                     /locus_tag="PKK81_00365"
     CDS             complement(20285..21394)
                     /gene="holA"
                     /locus_tag="PKK81_00365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNV31346.1"
                     /translation="MSASVMSLELPQSLARSGVMPLYAVVGEEDYLRDQSVAALRAAA
                     LGPAADTGFNYDIFHGDDCSVEDVLACAAEIPVFAERRVVVYKSVEKLPAREGEKLLS
                     YFSAPNDTTTLIVVGVKLDGRMKWTQALTKGAVTVNCAPLRDAQLSAWIRQEAAALGV
                     RIHEEATQLLKEVGSESLYAVKRELEKLAAYVPPDRTVQPADVEALRGTESGASVFDL
                     MNAIGAHDHGRALRILARNLENGEAPLRILGALVWQYRRLWKMKEQLKAGGREGEAAR
                     TLRMDPSRVRGFLAQFSDVQLTHTFRWFLETDSKLKGGSGSAPVRVMEDLLFRLCGQP
                     SKSASSAARVQPAPPVPRPSGSRPVSNVRTVTRKR"
     gene            complement(21415..22041)
                     /gene="lptE"
                     /locus_tag="PKK81_00370"
     CDS             complement(21415..22041)
                     /gene="lptE"
                     /locus_tag="PKK81_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNV31347.1"
                     /translation="MVWLIIFFALSVSLPGCGYQFRVQGAGPTVGGAPEAATTRPHAP
                     RLAILPIANSTYEANFEVKLANYLRREFSAGAGAEIVGPSAGADLYLSGQIMQITLPT
                     LSFDQTTTFESRVEMLVSVRIEEAKTKKVVWYQVAKGTSEFFLTQDLQFNRVLQNRAL
                     EQAGRFVAEDLASRFLLFLDTGELDKALKRPVKAEVAPAGGTPPYGAR"
     gene            complement(22109..>22980)
                     /locus_tag="PKK81_00375"
     CDS             complement(22109..>22980)
                     /locus_tag="PKK81_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNV31348.1"
                     /translation="DPAKERYWMPVDLYIGGSEHAVLHLLYSRFWHKVLFDAGVVSTP
                     EPFKKLVHQGIVLGEDNQKMSKSRGNVVNPDEMVDQFGADAVRLYEMFMGPLESMKPW
                     STRGVEGITRFLDRVWRLMIGDEGALSPAVTDTEPTPEQLRLLHYTIKKVTDDIDALR
                     FNTAISQMMVFTNEMTKLEQRPRRLLEPFVLLLLPFAPHLSEELWERLGQQPSAGQQP
                     WPQFDPALVVSDRLTIPIQVNGKLRGKLEVDAGASREQLEPLAKKEVAEWLQGKEPKK
                     VIYVEKKLINFVV"
     assembly_gap    22981..23080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23081..24529)
                     /gene="leuS"
                     /locus_tag="PKK81_00380"
     CDS             complement(<23081..24529)
                     /gene="leuS"
                     /locus_tag="PKK81_00380"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNV31349.1"
                     /translation="MSKTYDHQALESKWQTYWETHRPFRAVDDHSKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIVSRYKRMRGFNVLHPMGWDAFGLPAEQYAVKTGVHPAITT
                     AQNIATFKRQMKRVGLSYDWERELSTTDQDYYRWTQWIFLKLFERGLAYVAEVPVNWC
                     PALGTVLANEEIVDGKSEVGGFDVIRKPMRQWVLKITAYAERLLEDLTLVEWPTSTLE
                     MQKNWIGRSIGAEVDFPLADKTGNLRVFTTRPDTLFGATYMVLAPEHPLVDMVTTPEQ
                     RAQVTAYRDAASRKSDLQRQELDKVKTGVFAGGYAVNPVNQKRLPIWIADYVLMSYGT
                     GAIMAVPAHDERDWAFATQYHLPIREVIEGGQVDKAAFVETDRGRVINSTTPDGSCSL
                     DGLLPSEAIPKMTAWLETQGRGKKTINYKLRDWLFARQRYWGEPFPIVWVDGEPRPLP
                     EEQLPLPLPETKNFKPSGSGESPLANLHDWLET"
     gene            24756..25400
                     /locus_tag="PKK81_00385"
     CDS             24756..25400
                     /locus_tag="PKK81_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV31350.1"
                     /translation="MYATYIFPRLMDWVLRGERFQTERRLLLTPVHGVVLEIGFGTGL
                     NLPHYPTTVTALHTVDPAPLLPDRVAVRVAQASFPVHIQHVSAERLPYDDASFDYAVS
                     TFTLCTIPDPARALRDIRRVLKPDGHFLFLEHGRSDDPLIARWQDRLNPLQHLLACGC
                     NLNRRIDQLVLDAGLRLEQLDRYCLPGVPRIGGEMYRGTARLNGQSSLHSSLDT"
     gene            25662..29504
                     /locus_tag="PKK81_00390"
     CDS             25662..29504
                     /locus_tag="PKK81_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV31351.1"
                     /translation="MTLLYEAMPAALVAGLGCALALIAVNWHVVPHDRLLVWLVYHLI
                     LAAGRYGLARTFTADRPGPDNIERWDRAFLVGTTLAGLGWGASALLLFPESSPAHQVF
                     LAFVLAGITAGAASTLAARFDAFLSFSLPMLTPLILRLFALDHEQALPMAICTMLFSL
                     LMVYTAYRTSNTVLETLRLKYHNAQLVDQLTGQLQEGEQMELLLTVKTEHHRFIMEYA
                     QDIIYRTDRSGRFTFINPAVIRLLGYHETEMLGHRALDFVHPDHRRPTERFYLRQFLR
                     KTASTYCEFPLITHTQQTLWVGQNVQLLQRDQEVIGFQAVVRDISIRKQTEEALRVSQ
                     ERYRALFESSPEMLLTVDARGTLLTVNTTTAAELGYEADELIGQPVARIVHPDDHGSM
                     DKHFADSLRRPGEVLRWNFRKMRKDGSLLWVREAARAVRNSDGQFDIIIVCENVTEQK
                     GIEDALTQTRQLLESIVEHIPHMVFLKDAQELRFVQFNKAGEDLIGLPREALLGKNDF
                     DFFPLEQAEFFTARDREVLTGGTMMDIPAEPIQTKDHGLRWLHTKKLPLYDQAGMPRY
                     LLGISEDITDRKQREEAERLRLGKLETQQAVLHELAEDPAIHSGHPQQAFPVITEYAV
                     STFAVERAGIWLFGENQSALILQDLFEATPKHHLQGTTLSTGEYPAYLHALETEPYSL
                     AAHDAQTDPRTKELTPSYLTPLGIVAMLDAPIRRHGRVVGVLCLEHIGPARVWTTEEQ
                     TFAASLAAMATLTLEASDRRQAQEALEQHVRERTGELRRTTAHLQTIIEESPLAMIEL
                     DQAGLVTTWNAAATALFGWTKDEVLGKELPYVPPGQEKVSEELWTAVMSGAAPRNLEL
                     CRQKKDGALVDVNMWGSLLQSPTGQAVGSIGFFVDITKHKQLEDQLRQAHKMEGIGRL
                     AGGVAHDFNNLLTVINGCATLVLDQVRAEDPLRRSLTEILTAGQRAAALTRQLLAFSR
                     RQVLTFQVFDLNEALSSISSMIERLIGEDINLTRNLDSRPCTIRADRGQIDQVVLNLA
                     VNAKDAMPTGGTLTMATQALLITPDSPVPHPALLPGAYAHLSIRDSGQGMDRTTLSHI
                     FEPFFTTKEEGKGTGLGLATVYGIVKQSQGFIFADSTPGAGSTFDLYFPLVEAPPLAA
                     APTLPSRPHCGLETILLVEDQHAVRLLLTQALSDYGYTLLEASSGQEALRLVAAARTP
                     IHILLTDVVMPQMTGPALAEHLRRQWPELRVLFMSGYAEGSVLPTFLAQPGTGFIQKP
                     FLPTELAQKLRELLDPAK"
     gene            complement(29551..30237)
                     /locus_tag="PKK81_00395"
     CDS             complement(29551..30237)
                     /locus_tag="PKK81_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNV31352.1"
                     /translation="MLSNRSEAAHQSVDWSRIDDVLLDMDGTLLDRHFDNFFFEEELP
                     RRYAAKHALTFEAARDRLMAMYRSVEGELAWTDLHYWSERVDIDVVAMHRELDHMIGF
                     LPDAEEFLSSLRRLGKRVTILTNAHRAGVDVKTAKTGLDRHVDRIVDAFEVGWLKMRS
                     EYWPTCRQLVGFDPARALYIDDDEQCLAAAEDFGIGRIFHRSRSSSQASAVPSARFTS
                     IEHFHAILPG"
     gene            30298..31692
                     /gene="der"
                     /locus_tag="PKK81_00400"
     CDS             30298..31692
                     /gene="der"
                     /locus_tag="PKK81_00400"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNV31353.1"
                     /translation="MLPSRTMSRTKQTRGPVAPLFTLEGGRLPIIALIGRPNVGKSTL
                     FNRILGTRAAIVDDVPGVTRDRNYADSTYRNRRFRLVDTGGLDPTASDGMLSLIRQQS
                     QLAIAEADILVLVLDARSGLTPADEEVVQTLRGSDKPVYYVINKIDTPKADPLVADFY
                     RLGQEQLYPLSAEHGIGVAELLDDLFSHMAEPLDEEATSDTPRIAIVGRPNVGKSTLV
                     NSVLGEARVVVSDVPGTTRDPVDSVATFKDRQYVLTDTAGIRRRGRVERGIEGYSVAR
                     SLRAIGRSDIAVLLLDAEEGVTEQDTKIAGLVLKQGRACILIVNKWDLKTDDVHAREA
                     YKQDLERRFPFLAWAPVLFISALEQDFLRGLFALIDQVYFSFCKRVPTGPLNQFFQGL
                     LEEHPLPVRKGKPAKASKSAFMTQVATRPPAFALFVGHPDNMTPAYLRFLENQIRKEY
                     HFSGTPVRLLTRKK"
     gene            31919..32314
                     /locus_tag="PKK81_00405"
     CDS             31919..32314
                     /locus_tag="PKK81_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31354.1"
                     /translation="MFIGQPDLAAPAAPTSSVETGLIVLSMDGRILHTNDRAREFARH
                     FTNEPRRLQAPATTPLRHPLMDLFHDVLANLEKHIAAEDWSQFEITRLARWGGGTVRL
                     RGFGIPDQARRQQSRVVLTVHYQSSPSPW"
     assembly_gap    32353..32452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32453..32971
                     /locus_tag="PKK81_00410"
     CDS             <32453..32971
                     /locus_tag="PKK81_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31355.1"
                     /translation="TKIAGRQDHQPLTIEVDGTGTIKGLHDVQFDLQVNQVPRTPSSL
                     HLLLNGTVSKLKSNYVFLKTPLGIVTVSGKTGVRNAKVGQEMSVWIQDRHVAIDLYQD
                     GLTSPSRRFLTGPLAYGSDAHDTLTMQGPEGAQSIPLTQRPAALATLKEGLPITVELN
                     QQGELVDIRRVN"
     gene            33049..33681
                     /locus_tag="PKK81_00415"
     CDS             33049..33681
                     /locus_tag="PKK81_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV31356.1"
                     /translation="MASRKNSRPEIPDGRGVSPSTSAPFDQLYRDHVDVMYRFAYRLC
                     GEAEAAKDLVQETFLNAYRAYDRFRGDAQVSTWLYAIASHACQRMRRKRKGEPERELS
                     LTEFVPTSEGEFSLQIPMEGLSPQEALENKELRQILDRAIAKLPRKYRMVLVLRDMEG
                     LSAKEVGSILGLNERAIKSRLHRARLFVRKELSGKGLATEFHPDITTGHR"
     gene            33711..34010
                     /locus_tag="PKK81_00420"
     CDS             33711..34010
                     /locus_tag="PKK81_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNV31357.1"
                     /translation="MADRVRTYPGTRKPTLHQGHGKRNCVKVLERLSAYLDDELSGDV
                     CQEIRAHLGDCPNCEVFLDSLRQTVQLCRNRPSPALSKNDRANLRRQILKAAATA"
     gene            34126..34503
                     /locus_tag="PKK81_00425"
     CDS             34126..34503
                     /locus_tag="PKK81_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV31358.1"
                     /translation="MSDRLLLALLLTCGGTGVLLAQPSENFDHGQAIYREHCMECHGT
                     TGKGDGVKAPFLSPRPGNLISAATSAKTDQELLRTIAQGKPRTAMPAWQNVLPAEDQQ
                     AVLQYIRSLVRFARPLTPPPPGP"
     gene            34591..36312
                     /locus_tag="PKK81_00430"
     CDS             34591..36312
                     /locus_tag="PKK81_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31359.1"
                     /translation="MIRGNLFHRRQTIAIVTLALSLGLWAGTAESAPATGGKGHRPTA
                     SAKQPPAVQTAVRYAEALAHGDRVTAGQLDFACQYRYVVASPAKVKQFAPANDTSYDS
                     CWQTLTDAYAPMLKRSDIAMDIIWPSTGPLMFFGDDLPRAPASTFVADVLGTSPPGSG
                     LHVTVVGSRTIPSGSFRLGRAGKVLGVPTTLVQLSVQYQDPLTSPVTYAPGNVKWTNT
                     IKRPRLALKSVTTQWVVFTGLKQHGFPGDTAVFNLPVASQPEAPGMMADKIPFATETS
                     RALPESLAWWGPNDQPGTLTAAAARAAIFPDLRDRVALLNRVLLIDPKQPDALTVLTR
                     HLYSVLLREARNSHQVPIKDPALGIVVDEFYWNIYAQGARLDLSNGMEMGGLSQPTPA
                     DFLYRLIPALQTLVSVRPEQLDNRFRLGIAYRWNNDQLPMVETFESLVKDIPEHRRTP
                     KSEALLQLAWSRINKVSWNRILHDTETPRAYADAEAALAQAELPLDKFLAEYAMAYTM
                     IFLPNYGDKAKMLQHLTEAKRWFDEVPGKSDEVWRYFLHSELLKAVLDADPMFQPILA
                     TAAAPHA"
     gene            36421..36864
                     /locus_tag="PKK81_00435"
     CDS             36421..36864
                     /locus_tag="PKK81_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNV31360.1"
                     /translation="MSTEESMPVTARTASASRCAIVATLIASHFSLTGCSIFGGSTQP
                     LMVNSEPPGAQVLINGTAAGTTPLQYQVPRRGDLTVEVHKTGYQTQSRVTGRKLSSTG
                     IVDVIGGALFLLPLLGLIAPGAWEQDPSTLGITLEPDTHQPAQAP"
     gene            36916..37962
                     /locus_tag="PKK81_00440"
     CDS             36916..37962
                     /locus_tag="PKK81_00440"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNV31361.1"
                     /translation="MIDVHGYAALNAQSPLTPFRFSRREVGKQDVLIKIQYCGICHSD
                     VHQARDEWGGSLFPMVPGHEIVGTVEKVGASVKQFSVGQTVGVGCFVDSCRTCQACKK
                     GLEQYCEGHLAFTYNGKERDGVTPTYGGYSTKVVVDQRYIVRIPKRLRPEEAAPLLCA
                     GITTYSPLRHWGVGKNHRLAVVGLGGLGHMAVKIGKALGAHVTVLSHSDKKQADAKRL
                     GAQAYYSTAKPETLTQLTKRFDFILDTVSAPHDLNAYLELLKTDGTMILVGVPDKPAQ
                     LGAFPLIMRRRRLVGSLIGGIKETQEMLDFCGTHMFGADVEVIPIQQVNAAYDRLVRG
                     DVRYRFVIDMSSLT"
     gene            complement(38156..39121)
                     /locus_tag="PKK81_00445"
     CDS             complement(38156..39121)
                     /locus_tag="PKK81_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNV31362.1"
                     /translation="MTVRNIRFRQSVIRTVLGLAAVSGLAMQPVESAFAKVVEVSMTA
                     VETKLVIDGEGHTYDAWTFNGQFPGPVIRVQEGDEVVFSLTNPETNGRPHSMDFHAAE
                     TNFLEHYKEVRPGESLQYRFKAKWPGIFAYHCGASPMIQHIARGMMGAIIVDPKDPKA
                     LPKADREYVLVQSELFKDPDDVDGMFDRKYQHVVFNGSVFRYDPVHSKAGGDFLDAKP
                     GERVRFYFVNAGPNNVSAVHPIAEIWDDVWESGNPANHTRGVQTQLIPPAGAAILDMV
                     LDNNDGVYPIVTHSLTDALRGAIALLKVDKDARQLPLMPFVTPAK"
     gene            complement(39278..40642)
                     /locus_tag="PKK81_00450"
     CDS             complement(39278..40642)
                     /locus_tag="PKK81_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNV31363.1"
                     /translation="MSDLQRQLDTYIKDIRPRYEDMLGQAVEIPSISMDPRHTPDVRR
                     MAELAAQYLRAAGAETHIVETPGYPVVSGGWTVDPSYPTVTVYNHMDVQPAQEPEWKQ
                     APFAFQNDNGIYRGRGATDDKGPALAALFGARYAIEQGVPINVRFLWELEEENGSPSF
                     AAAIKNRAAIPRPDSVVISDTIWLSKNQPAMPYGLRGLLGARLLLRTGSKDAHSGVTG
                     GAARNPLAELMDIVHACVDAKTGKVKIPGFYEDVVEPTKAEIKSFLQSGFQVSKFKQA
                     YGFKTLRTQDPAEVMRRIWAAPTFEVHGLVGGYHGPGVKTVVPGHGELKVSMRLVPNQ
                     TPEKAFALLKKHVAKLNPEVTVEREGMLHPFRGVFDGPYVDAVKRAVKAGFGKEPAFI
                     REGGSIGAVVTMQKAWKVPILFMGLSLPEHGYHAPNEYFDWGQASGGMKAFVHYFAEL
                     AAKR"
     gene            40731..41219
                     /locus_tag="PKK81_00455"
     CDS             40731..41219
                     /locus_tag="PKK81_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31364.1"
                     /translation="MRSMIHDGSQILNRSIAKLLFGLTAFLVLHTDMVAAQEAVTVQE
                     ILTDPALFHLRQITLRGTARNVQPLDPYEIPAGSTCYGGYLFNLEDDTATLPVAVPGL
                     CGVPMVKDPDVEDGARVVVEATVQAPSHGGYALSFKGGKIAMDQEGVVQAIANRIMPL
                     VE"
     gene            41223..42173
                     /locus_tag="PKK81_00460"
     CDS             41223..42173
                     /locus_tag="PKK81_00460"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV31365.1"
                     /translation="MALPLPEQSPRLVTPAQTHVGWIGTGIMGAPMCQHLQANQYRLT
                     LYTRNRSKASLILAKGATWADSPRAVAEQTEVVVTMVGFPRDVRDVYFGKQGILAGTR
                     PGMVLIDMTTTEPSLAQEIAQAAQSRGAFTVDAPVSGGDVGARNATLSIMVGGTTNAV
                     QAIMPLLQCLGKTILHQGGPGTGQHAKLCNQIVIAGTMIGVCESLLYGYKAGLQLDRM
                     LESIRGGAAACWTLDNLAPRILARNFDPGFFVEHFIKDMGIALEEAQRRQLTLPGLTL
                     AHQLYEKVQALGHGRSGTHALMLALEDLSHINPVRPSAST"
     gene            42187..42918
                     /locus_tag="PKK81_00465"
     CDS             42187..42918
                     /locus_tag="PKK81_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31366.1"
                     /translation="MRTVPLRILLLLLLSVTVSACTGARGLNRQLLQESFHDHPEVVT
                     DRDIASTMTLQANLPTPYRLAVFFKQEDFPSRPALQRVDWVSTDADRVQRALAPVQEE
                     GILQQSFLLANSTVQGTTLRDIRLAAARYNADALLIIDGASAVERYNNGHAAWYATGI
                     GAYFAHGTESHALFMMEGTLWDVRTGYLYGTQTAEGETTLVGPAPSLEDRTAVAEAKD
                     VALERFSKELAEMLRLLREKQRAMK"
     gene            complement(43138..44364)
                     /locus_tag="PKK81_00470"
     CDS             complement(43138..44364)
                     /locus_tag="PKK81_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNV31367.1"
                     /translation="MKPALLLHNDVGSAMETAPAGFLRRLWWVLLLCTLVVPAHAGEI
                     SIAPDPIPATGTDLWHYGAYLDVGYVVNPNFPENHLWRNRATAARHNEFAPNMALAYV
                     RKDATESSRWGMELGVQGGYDSERFAFLQGEREVGGADTLRHIHRANVSYLAPVGKGL
                     TITAGLFNSLIGYESLYAKDNVNYTRSWIADNTPYMMFGVNAKYPVTDKLTVAGFIVN
                     GYYHLSHPNDQPSYGGQWAYKATPQLTLTQTVYGGPDQTNTALQFWRFYANHIVEWKG
                     ENLTFAASYDIGTENIADRPGNPRAFVMGGNVVARWHVTGPWALAVRPEFYWDRNGRW
                     TGSEQFVKAVTSTIEYRIPYKWTNTTLRLEHRWDESTGAGGGFFRRGEIQPGVLSLTP
                     NQHLVLLGILWTFDSP"
     gene            complement(44479..45186)
                     /locus_tag="PKK81_00475"
     CDS             complement(44479..45186)
                     /locus_tag="PKK81_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31368.1"
                     /translation="MSSTRSHDVTVLLIEDEAEIRRFLRSTLPAHGYRLYEATTGADG
                     LAQASARNPDIILLDLGLPDMNGTAVIEKIRAWSNMPIIVLSARDQEQVKVQAFDLGA
                     DDYVTKPFGVDELLARMRAALRHATRPVGDGVDASCTFGDVTVDLGRRQVFVSGAEIH
                     LTPIEYKLLTTLVRYAGKVVTHRQLLKEVWGPLHVDEGHYLRVYMRQLRNKIEADPAQ
                     PRHLVTELGVGYRLRTE"
     gene            complement(45200..47908)
                     /locus_tag="PKK81_00480"
     CDS             complement(45200..47908)
                     /locus_tag="PKK81_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HNV31369.1"
                     /translation="MENQRPDPDALLRRVQAEEAEQVRGKLKVFFGATAGVGKTYAML
                     QAAHEQQRDGIDVMIGWVETHGRAETEALVQGLPVLPSREVVYGGSRLRDFDLDAALS
                     RRPQLLLMDELAHTNAPGSRHPKRWQDVRELLQAGIHVYTTVNVQHIEGLNDAVAQIT
                     GIRVSETVPDSILEQADDVELVDLPPDDLLQRLKDGKVYLPDQAQHAIKHFFRKGNLI
                     ALREMALRRTAERVDQQMEVYRRDHAVVGTWPAAETILVCVNLKSRGPMLVRAARKMA
                     TGLHARWIAVYVETSIHLRLTEQERSQGVDTLRLAERMGAETVTLTGDDVSAQLLAYA
                     RSRNVTKIIVGKPLRSRWKEWLFGSVVDDLVRGSGDIDIYVMTGEAGTGQPLVTGLLQ
                     RTSERRNYGYASALVMACTALSGVMAPYFGQANLIMVYLFGVVVIATRWGRGPSALAS
                     LMSVAAFDFFFIPPYYSFAVSDVQYLLTFAVMLVVALLISGLASHKSRQAESALIREQ
                     RTAALYAMSRELITQREVNKLASVASRHIHDVFHCQVAVFLPDRDGRVHLHRGDALHF
                     DLDPKEAGISQWVFDHKESAGHGTNTLSGSDSLYLPLLGSHGAVGVLAVRSSRPAELL
                     APEQLHLLETFANQMALALERARLAEETQEAHVQTETERMRNAVLSSVSHDLRTPLAT
                     IVGASSAILNTTRPLSIESQHELVRSISDEAGRLDRLLKNLLDMTKLEAGAMQLRKEW
                     HALEEVVGTALARVKSRIATHPVSTTFPDSLPLVLVDGVLIEQVLINLLENAAKYSPP
                     ASPIDVTASVKEGMIVTEVADRGPGIPRGEERRIFEKFYQLHPDREGGVGLGLTICRG
                     IIESHGGRIWVEARSGGGSSFCFTLPTEHNQPTVEPEQNGHDRAVT"
     gene            complement(47994..48575)
                     /gene="kdpC"
                     /locus_tag="PKK81_00485"
     CDS             complement(47994..48575)
                     /gene="kdpC"
                     /locus_tag="PKK81_00485"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNV31370.1"
                     /translation="MVVQLRSALMMFFILTILTGVAYPLAVTAIAQLLFPHQANGSLI
                     SKDGKPIGSTLIGQPFDDPKYFWGRPSATAPFPYNAAASSGSNLGPTNPALIETVKMR
                     VAALKAADPGNDTPVPVDLVTASGSGLDPHISPASAEYQVRRVARARGREEAFVRTLV
                     SQHTEGRQLGMLGERRVNVLALNLALDATDPPR"
     gene            complement(48586..>50600)
                     /gene="kdpB"
                     /locus_tag="PKK81_00490"
     CDS             complement(48586..>50600)
                     /gene="kdpB"
                     /locus_tag="PKK81_00490"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNV31371.1"
                     /translation="RNPVMFVVFVGSLFTSLLFLQALFGTGEAPTWFILAISLWLWFT
                     VLFANFSEAIAEGRGKAQADSLRRARTELTAKRFGKPTSGEDYLAVPAAIGKPGEVYC
                     LVPASFLKKGDIVLVEAGDDIPSDGDVIEGIASVNESAITGESAPVIRESGDRSAVTG
                     GTRVLSDWLVVRITASAGETFLDRMIAMVEGARRQKTPNEIALTILLSALTVVFLLAT
                     VTVLPFSLYSAHAMGEGVPITVTVLVALLVCLIPTTIGALLSAIGIAGMDRMIQANVI
                     AMSGKAVEAAGDVDVLLLDKTGTITLGNRQATAFISAEGVQERSVADAAQLASLADET
                     PEGRSIVILAKERYGLRGRDIHAMGATFLPFTAHTRMSGVDFDGRQIRKGAAEAIEDY
                     VTQLGGAFPPAVRNAVETIATQGGTPLVVAEGKSVLGVIHLKDVVKGGIKERFAELRR
                     MGIKTMMITGDNQKTAAAIAAEAGVDDFLAQATPEAKLKLIRDLQAGGRLVAMTGDGT
                     NDAPALAQADVAVAMNTGTQAAKEAGNLVDLDSNPTKLIEIVEIGKQLLMTRGALTTF
                     SIANDIAKYFAIIPAAFAGTYPALGALNVMGLATPESAVLSAVIFNALVIIALIPLAL
                     RGVKFRPIEASLLLRRNVLVYGLGGIVAPFIGIKLIDLVLVALGLV"
BASE COUNT        10496 a        16248 c        13966 g         9690 t
ORIGIN      
        1 cagcataagt cctcctcctt tacacaagtg aatgactccg cccgatggtc cccataaatg
       61 accggctcat tatagccaac gctccccacc atgtagagca aaaaaatctg tgggaaacag
      121 gggaatcaac ttgctcctca ttccacacaa tccctcttgg ctagaaaatc catccaatcc
      181 ccgccagggc gtgatgcgca tgataatcga gggatacgtc gctccagtga aagttggccg
      241 tcacccgttt gtattcggag aaaaggaagg tgcgcccgcc cagactatat tggaccccca
      301 ccatcagttg ataggagaac gcagcagttg aatcgaagtc ttgattcgtt cgccccggga
      361 aactcgctcc atggaaaatc gccgaggagt atcccacacc aacaccagca taagggcgaa
      421 actcgccgtc aggttttctg gcaatgaggt tagtcatact gttcgtaacg gtcagtcccg
      481 ctgtagcctc tcctctgccg ttgttcccag aggttgcagc caagagccta ccagtagttc
      541 caaaatattc gatttcgacc cccacgattc gccgggtgaa ctccgggaaa atgccaattc
      601 gcacccccgc gccaagactg ttgtataacg tgaccttggg ggcgccctgc ccgccaagat
      661 tcaaaccccg atcctcagga aacccaccca ccaccgccag gctcagatag acatcggcaa
      721 attctgccgc atgaaccgat gttggcctca atacgtgata ctgagcatac cctgcgagca
      781 aaatacttcc agccaccaga aacctgccca gaatgtagac cagggtaggc tgttgattca
      841 tgaacaaacc aacttctttc tccattcgtc caccaactca gactccatgc tccgatatta
      901 gcgtagtcgg cattcacgag actatgacac gcgaacaaca ggacaaaaaa aagcgtggat
      961 gacaatgatg ccatccacgc ttcatctgta ctgggaagag agtgtcgctc ggctagaggc
     1021 tagttccctt ttgcaatggt gcaggtctct acataaaggc cccaaacaaa cgacttgatg
     1081 tagtgattca caaacacagc cttcttaata ccaccagcat tcatggccgc ctcaacagag
     1141 gtgtcgccgg tcgccacgat gcccaagagg ccggtaccgc aagactttcc tgtcttggtc
     1201 gccgttgcac cgttatcgtg caagatgcct cccgaggttg tctccatata aattccggcg
     1261 gttgggaaaa tacctgcagg ctgcgtatat ccggcaaccc cgctacatcc atagagcgac
     1321 gcagcaaacg cgactacgcc taacgacatc agcatacgtt tcatacagac atcccccctt
     1381 tgtggttgaa attgaacgga cgagaaacgt gaactcctgc caaggactgt gggcttttcc
     1441 taggcatgac agctcaagtt ggcgcaacta tattcagaga ccacaagaat gtcaacaatc
     1501 ttcctgatgg agagtaaccc aacagcgccc catggaataa accttcaggg tttatcattc
     1561 cgcccgatcg gccagcataa tgtgccgcgc gtcgatggtc cctacatcga gcaggagcag
     1621 gcccactgca cgcggcaggt gaaaccgttt cggtcccgcc gacccgggat tgaacaacaa
     1681 caccccctcc tgccattcca ccttcggttg atgggtatgt ccgtagacac acatcgccgg
     1741 atgtgtccgc gtcaagaatc cagtcccctc gcgggtgagt cgcccccctt catacagccg
     1801 gtgatagagg gcaatccgat gtcccgccag ttcgagcacc tgttctatcg gaaacccgct
     1861 cgcctcgaac ccgtccacgt tgccggatac ggctgtgacc ggagcaatct gtgtcagctg
     1921 ctcgatgacg ccacgcttgc cgatatcgcc ggcgtgaagg atatgggcga ccccggaaaa
     1981 gtgacggagt aacgcccgat cgaacagtcc gtgcgtatcc gaaaccactc cgatccgcat
     2041 ccgcagctcc gcgattattt tttcggttcg gacgcgccca gtgaatcctg aacgcctttc
     2101 cactcgaagg tgaacggctc ggtggtctgc ttgggcaatg cggccagctc cgctttcaac
     2161 cgctcggcac gggccgcgct catggggtga gaggagaaga gctccacacg ttccttgtct
     2221 ttttccgaga gacgttcaaa gaaccgtatc atgccgtccg gagcaatgcg tgcgtcgtgc
     2281 aagagccgaa tgccggtgac atccgcttcc gtctcctgct cgcggccgaa cttcaacgtg
     2341 gccaactcca agcccagttg cctggccaga ccgatcaggc cttgctgatc tcccgtgaga
     2401 atactcacca ccgcggctaa ccccatcatt ttcacgaggc gttccatccc atgccgctgc
     2461 aacacgtggt tcaactcatg actcaatacc ccggccactt cttcgccgct ctccgccttc
     2521 ttcatcaagc cggtgaacac caccacatag ccccccggca acgcaaacgc gttcaccacc
     2581 gagctctgta ccaccgacac ctggaacgtg taggggttgt tgggaatctt agccgtcatg
     2641 cgctgggtca tttcctgcac cgcgctgacg gccgggcctt ccttgagcac cgtttctttg
     2701 gagaggaagt cctggtagac cgtttcgccg agcttctgtt cccactgaac cggtatgcgg
     2761 gcgaccgccc attcgacaat cagatccgaa ccgaaccaca acatcaatcc aagcccgacc
     2821 accaccccca cggcgctgcc ccacaaggca cgatggctgt ggcgcgcgcg ccgaacctgc
     2881 ccggctgccc gctcaagatg cgccatcaat gcagacggag cggcgcgacg aaacgcgatg
     2941 atcaatgccg gatccttgag atacagcgtc cggaccgatg cgccgactcc ccacgacgcc
     3001 accagctgat catgatccag cccccccgcc tcaacagaca tggccgtgaa cgcgatggcc
     3061 tcttcgacaa gcagagagtt cccgaccgga gccatggtca ggccatccgc cgacaccgtg
     3121 acatagcaag gcgcacccgc ccccggcagg ccatcaccaa aacagagcgc gttgggagta
     3181 gggttcatcg ggttcgatct tgcggctgat caaaatggtt gtgaacgagg ccgcaggcga
     3241 acgcaaaccg gaggcatacc ctcagcggta cgttgaggat ttgtcgtgag ccgagaacga
     3301 agtgcacgag cattttgacc ggccgctatt ccgaaacagg gatgaactgt ctgatccaat
     3361 tcccgaagct tccaggattg cgactttgga tatagaccac gcctggacca cggaagcggc
     3421 acaccagccc ttcacctgac gtcaggctgg caatccagcc ggaggaggcc ttttcgatat
     3481 tgtacgtggt ggtggaagtc caggcgacaa gatggctgtt atcgacgatg tactcttcgt
     3541 tcggcttcag ctcaattttg tggatcgccc cgaagctgtt caggatcagc tgaccggtcc
     3601 cgccgatctt caggataaaa aacccttctc cgcccagcaa gccgcgcgtg agactttgca
     3661 tcttgctttc gatcttcact ccttccgcgc cggccagaaa cccatccttc tgcaccatgt
     3721 attcattcac gccatcgaga tccagcaata caatttcgcc gggcaccgtc ggggccagga
     3781 gcacttctcc cgctccgcgg ctggcgcgca gcgtctggaa aaaaaatttc tcgccgctca
     3841 gcatcttccg cgataacgcg cccagaaaac cgccttccat cttgctttcg atatcgatcg
     3901 tcggcgaggc cgctaccatg gcgccggatt cggccttcac cgtctcgcct tcgttcagat
     3961 acacacgcac catcggaaaa gccccgggat acaaaatctc gctcttcatc tcgcactccc
     4021 ttcttgaaac cacagcctct gacaaatcac ccgattctgt ggaatgtccc ccatcctgtc
     4081 aagtcacgcg gcacttccag tgagccgccg gtcacccagc cccttcggcc ccctcaagat
     4141 cggtggagtt tactccgata gagaccacac ggacgccctc atgtccggag gggcgaatcg
     4201 tccccacagg gaatgccatt gtgactgacc tcccaggtga acgtatcgaa cacacgcgag
     4261 cgcacccatg aagcgcgtcg tcatcgaaga actcacaacg ggcatgattc tcgccaaacc
     4321 tgtcaccaac aatgcagggt tggtggtgct tcctgtcggc gccgagttgg atgaagcgac
     4381 cctctcccga ttgcagcgcc tcggcctgac gtccgtctat gtggaggggg aggcgggtga
     4441 cgcgagcggc aagacgctcg acgagctgga agccgagctc gaccaccgct ttcgactggt
     4501 ggcccaggat ccgattcaat gtctactgcg cgaggcaatt cgtctccacc ttcgaaccac
     4561 gcatggaatc agcgacaatc cggaggcgac gccatcggca tgaatccttc aaggaaaatt
     4621 gatctccacc gacgaatcga gcaagtcggt gagttaccga ccctcccgca tgtcgtccag
     4681 aaactggcct ccatgatcgg tcgccccaac gtatcggcgg aagagatcgg aaccctactc
     4741 gaaaaagacc aagtgctgtc cgccaaagtt ttgcggcttg ccaattcgcc attttatgga
     4801 tttccttcgc gtattgcgtc ggtcgcccac gccgtagtgg tcctgggctt gaacgtggtg
     4861 aagggcctca cgctttgcgc gactgccttc gacatgatgc gaaacgccgg catgaacgaa
     4921 ctctggcgac actcactggg agtcgcgatg accgcccaca ttctgggcac caaggcggcc
     4981 atgaaaaatc ccgaggaagt ctttgtcgcc gggctcctgc acgacatcgg caaggtcgtg
     5041 ctgtatgtca aatggcccga tgtcggccag cagatcacaa ccgtcacccg caacacgtcc
     5101 cggcccctca tggacacgga gcaggaattg ttcgaggtga cccatgccga tgtcggaggc
     5161 tggctcgcga gcgcctggca tcttccgacc agcctgcgtg aacccattct ccatcaccac
     5221 acccctgcgg ctgctcagga tgcgaagctc caaactgcca tcgtgcatgt cgccgacgtg
     5281 ctggtgaagg gcctggcctg tggcaatccc ggcgatgacc tggtccctcc gctgtcccgg
     5341 caagcctggg atctggtggg cctggatgcg cagagcctcg cccactgcct tacccaggcg
     5401 acggaagagt tccagaccat cgacgactac ttatgagcgg gtctccatcg aatcggcgca
     5461 ttttgttgct gcacagcgat ccgcttgaaa tcgagcggtt aacgaccggg ctgaaccggt
     5521 ctggatttac ggtggtggca gcggatgcgg gccgcctggc gatgcacgaa ctggtgcatg
     5581 atccgccctg tctcgtgctc gccgccgaag gcaccaacgg ccgctcggca gacacgttag
     5641 cccgggactt gcgcgccgac cccttcctgg gacggttgcc cctgatcatc ctggtgcgtg
     5701 atatccgggt caacgatttg gattgggcga cgctcggggt ggacgactat atcgccgtgc
     5761 cctaccgtcc cgacgaagtg ccgcaacggg tgcgcctctg cctgagccgg cttgaacgct
     5821 cgctggacgc caatcccctc accagactcc ccggcaacag cacgattctc catgaaacga
     5881 ccgcgcgcat cgaaagccgg gcacccttcg ccttggccta tctggatatc gacaacttca
     5941 aatcgttcaa cgaccggtat ggctatgccc gcggcgacga agtcctggta gtcacctgcc
     6001 gaatcctcac gaccgtggtc agcgaactga ccggtaccga cggattcgtc gggcatgtgg
     6061 gtggagacga ctttgtgttc atgagcgctc cctccgccat cgatgcgatt tgccagactc
     6121 tcatcaagcg gttcgacctg gtcattccgg atttctacga tccggaagat cgacaggcag
     6181 ggttcatcga ctccgtcgac cgccgcggca accacgaacg atttcccatt atgagtttgt
     6241 ccatcgccgt ggtgacgaac gaacatcgcg acatctccca cccgggagat gtcagtaaga
     6301 ttgcctcgga attgaagaag gtcgccaaga gccggcccgg cagcgtctat gtgaaggaca
     6361 accggacaaa tcccccggat tcagcccctg aatctactcc ccgctaggtc ctcatgaaga
     6421 gcgtcccccg agggagtccg agcgaaacac ctcaaccgtt acggcgccga cgatccgccg
     6481 ggggcctgcc caccggcatt cccgccatac tgacgacgaa gttcacgccg gtacgcgcgc
     6541 cgttcctccg gtgacatgtg tttgaatttt tcccgcagtt ctttccgctg ttcgggtggg
     6601 agggacctga accaccgccg ggcatcgcgc accgactcgc gttgctcagg aggaagctgg
     6661 cggaactgct gataccgctc gcgtagctgt tgctgctgct ccggcggaag cgtccgccat
     6721 tgtttgaacc gttgcttggc ttcctgacgc tcttccggcg tcatcgtccc ccactgctgc
     6781 gccccgttac gcaaccgttg ttgtttacgc gggggcagct gatcccagtt gtcactgaac
     6841 cgttgcagca gttgctgctc gccggggctc aactggctcc acggcacgcc cgccgaatcg
     6901 ccctgcgccc cggcagaggc cacgccgcat gccagcacga ttacacacac cagcaacgct
     6961 ctcatggtcc ggtctgcatc ctcatcggat caggtggcac gttcaaatta cgcttctgct
     7021 cgccgccctt cgacggtctt cgaggacttg tcgctgggtc tctcgtatcg tccttcgaag
     7081 tcggacgttc gtctctggga agatcctcac caatttggaa tggatccacc cagcggccgg
     7141 agtcgttctg ccaggatccc agaaagtcga gaacatcgac atcgacctct tccggtcgcg
     7201 gcgccggctc cggagctgcc tgggccagtg aggcacacag acacagaccg agccacagcc
     7261 aaggactagc cggtcgtggc ggtgccatcg gccagccagt tgtaaaattc gagatcttcc
     7321 gacaattcga tattttcagg ggattgcatc agttcgagat cttcgagcag gaggtgggag
     7381 tgattcgctc cgttggattg ccacagccag atggtgacca tcagcgtggc catcgatgcc
     7441 agcgccacac ccccggccca ccgaagccaa ggttgccggc gccgcgaagc cgcgtccagc
     7501 gcagccagtc tggcccgctg caccgacagc actgtcttct gatcgagatc ctgcacactg
     7561 cgatcgagga ggtgtttcgc cgcaaccgcg agcggatccc gttgttcatc cgatcgttcg
     7621 ttcatggcca atgcgctccc aatcgcgttc gcaacacctg caacgcccgg aacaaatgcg
     7681 ttttgacact gccttgcgaa cagcccatag cttgagccgt ttgtgcgaca tccagttctt
     7741 cccaaatgcg taacagaaac gcctgctgct gtcgcagcgg aagggctcgc agcgccacat
     7801 ccagctccgc acaagcacgt ttacgctgca cgtcctcatc cggcgccacg gcggtcgaat
     7861 cggcaatttg ctccagggga tcctccccct cctcctcatc gtgagggctg cggaaaaatt
     7921 cccgcaggcg gttgcgcacc ctctcgcggc ggtaccagtc gcggatgcgg ctctgcagaa
     7981 tacagtggaa cagcgggccc cactcctcct cggcccgttc gccgtacttg tgcgccagct
     8041 tcaacatggc atcctgcacc agatcgagcg cctcatcctt attccccgtg gcaatatgtg
     8101 ccatgcggaa tgcgcgccgc tcaatccctg ccaggaaccg atccaatgcc tgggttcgat
     8161 ccagtgatcc caccctctca cccaggcaca gactgcctgg ctgtccatcg acggcaatct
     8221 gggaatgaca gccgttcatt cccgcctatc cacttccacg cggcaacctg ccagaccgca
     8281 atctccaata cctgccgaca cgaggaccgc ctgctgcggg caagtcccat attgagcact
     8341 tctacgaacg cagatacggg tggccccggg gaatctgagg tcggttcgat tctcgacccg
     8401 aacgaccgtg aaatcaagga aaggcaggca atgcaccatg gcatcgccga ccatccgctc
     8461 gtccatcccc tcttcccgtc tctggatagt cacaccctct cccctagacc gcgtgctagg
     8521 agaggacaac gcatcaggat gcttccggtt gaccgggctg aaaaggaagg tggaagggag
     8581 ggtcggcagc agtccagaat gtggttgaag ggatcgggtt acttggattt atcggcgtct
     8641 gcaggccgct ttccaccggc ccatgcgacc gagggaattg gcgccaccag gtcgaaccga
     8701 tcctgtgctt cattgtcctt caagtcctgg ccgacactat agagaacgcc cttcttcata
     8761 ttcaccagca tggggaaacc ggtgaagggg tcgtagacgc cttgccccgc cttggcaatt
     8821 ctggtgagca ggtcctgttc cggtggtgtc cggcgtaacc aggcctgcag ggatgccagc
     8881 cgtaagcgcg catccgtttc cagcactcga cctgcgtagg tctcccatgt cggaagggga
     8941 tccaccccga cgaggctttc aatgggattc atcacgatgt ccccgagccc atacgggggc
     9001 gcgtgcacga actgagactg tttcggcaaa tcggtgtacc gcccctctgc agcagccttg
     9061 cccgcggctt cataatattg cgcataggcg ttgaaccggc gctgcttcgg caacggcaac
     9121 gcggccgcga cggagccgta gaacggtcgg gcatcggacc ggtcctcgct caccgcttcg
     9181 tccagcgtct tggtggccag cacgaactga ctctgcatcg gccaacggac cgactgctct
     9241 gcctgttcaa gcggccgggc gagtttggcc aatcggctga cgaaccggtc gtcaagttca
     9301 ggccggagca agagtccgct catgaccaca atgtcatcat tcatcgcagc actggccagc
     9361 atttttaccg gcagcgtctt ggcctgcccc aacaccgtcc gccaggccgt cagatcggtc
     9421 tcaagtcgtg tgaccccggc ctcgacatcc tgcgcaaacc cctcggcgat atagagtcga
     9481 tgggcatgga gaatcagacc acagttcggg gtgatcgatt gtccatagcc ccagtcttca
     9541 aagggcttcg tcaaccactg ccggtatcgg cccatgctga cctcggcccg ggatgcccat
     9601 cccttcacac ccgtcgcctc cgcgcgcatc tgcgccgccg gatcggcagt cttcatccac
     9661 ttgcccgcca actccgccga agccccctgc tccccgccgg atgatactcc ctcaccggtc
     9721 aggcaagcgt gggcataggc acgatcggcc ccctcaacgc gccggtccat cccggcctgc
     9781 acaggatcct gagacgccgc tgcaccgaat ccaaggagaa ggacatatcc gttcttgctg
     9841 gaatcctgca atgtacgttg cggcaccgac gtcatggtgc gataggccgc gctgggctgc
     9901 tcctccagga ccagccatgt cagtgcgacc acaccgaccg ctccgccgat cagtaccagc
     9961 gtgagcccga ccagcgccag gaccatgacg actaacgatt gggtcgtcga caccccggct
    10021 ccaaccagag aggccaccag ctcgcccatt cgagtccacg cgccggctcc gacaggctga
    10081 ctcggcttgg ggcgagcaac aggcgcgtcc acgggcatag gcgtcacagt ttcctgttcg
    10141 tcgggcaggc gttccaccgc cacaacggcg ggtccctgtt gctcgacaga caaggcggga
    10201 acaggagccg gggcttcatc gggaatggtc gctgggggag tagcttcagc aacttcagcc
    10261 agaacgggtt ctgcgggctt cttgagccag cgtgtcagca tgttcccggt tgaggcgctc
    10321 ggagtcggct tagacgacgt gtcatcccac agaatccgca acccctcacc cgcgtcgggc
    10381 ggagaaacca cctgagccgt tggaggcggc gcctgctcct ccgaaggagc cacagactca
    10441 gcgggtgcct ccacgagttc tggcgatgga gagacgaccg ccgcttgcgt ttgctcgacc
    10501 ggaggcaaaa ccggttcctc cgttgcgaca acgatcggca gaggactcgg cggaagtgag
    10561 gctgcttcgg gagacggcgc gaccgggttg agcgatacct ccaccggagg aacaatgggt
    10621 tcgcttatgg gaaccgtgtc aacttcacga gcctcagccg ctgatggaga ggatggctcc
    10681 tgatcggtca acgccatctc cggctctgct tgaggtgtca cccccagaac aaccggcgac
    10741 aagggggcat ccggtgcaac cggcttctcg tccgctaggg aaggcgcggc ggcaggaaca
    10801 cgactctccg cgagcgcctc cggcgcaagc tcgactctga cctcctgtgc gcagagaatc
    10861 tccgcgggct gctccaccgg caggactggc tccgacggca gttccgcaac cgcctcgccg
    10921 accggtgcct gaggctcttc gacggtgacc tgctccggtt ccatcggcgt cacgtcggtg
    10981 ttgacggcaa tggcaaactc cgtcaccggt tcgactgaag caggaggtgg ttcctctaaa
    11041 ctcgtggcga caaccgactc actgtcggcc agacgcaacg gagcttccac cgctcgatcg
    11101 atcgtggatt cggccggttg ttcagcttca ggcacggtgt ccggattcgg cgcctcgacc
    11161 gccttgtagg gctcgactgg aatacgaata gaatcctcag gcgcctcggt cggagcatcc
    11221 ggggagagaa tccgcaattc agcggtcact gtggcaatag gctccacagg gcgatcagcg
    11281 acgggctccg ccaaacaagc ctctccagca accgttgcct cagtcacgtc tggcgctgca
    11341 ggcaacgcca ccgtggagtc ctgtactgcc acgacaccct cggcaaaggc cgggccaaat
    11401 tctggctcag gctcctggcg cggaatcgtg acatcgtcca cttcaatctg ttcccatggc
    11461 atgggcgccg acaaggttgt ggctgcaccg gcctgatcag ccggaatgtc cgggaccgtc
    11521 gcctcgaaca agggggactc ttcctctgga ggagctggcg gagccgtgag agcgccagcc
    11581 gtccgctcct gtaccgcagc atcggacgca ggcttctgct caccatccga aagcgtctga
    11641 gcgggcgcag gagacgtttg catggccgcc acagccgcaa gaatttcctc ccatgagagt
    11701 cccgatgagg tgagttccga aggacgcgat gaagaaaccg actccgcggc ctgactgacc
    11761 tcggccgcct ggtttggcgc aggagactcg atcgccttct caacctcgtc cgttccaggc
    11821 tccatcaggg tctcagtgcg cggatccaca gcacccgctg cttcttcgat ctgatcccag
    11881 ggcatcggcg caggacttgt cggcgcttgg gcaataggat ggctactggc ctgcgtgagc
    11941 acttcgatcg tcggcgcgat gggctcagac actggtgcct ggaggtccat ctcttcgaac
    12001 ggcatcggag cggcggcagc cggcggttca ggttcgacag gttggaatgg agctaattcg
    12061 gccagcggca gcgttgagac ctcgctctgc gttggcggca acgactcggc gtcaacaatg
    12121 accggttccg gactcgacgg tggcgagggg attacgaccg caacctcttc gatctggtcc
    12181 caaggcatgg gggcagggct cgccggtgct gtggcaatcg ggtgcccact ggactgcgtg
    12241 aggacctcaa tcgacggagc tggcgactcg gccactgatg gcgtgaggcc cacctctccc
    12301 gaagggggag gagcaatgtc cgccggcgtc gccgccggtc cctctggctg cggaggaatg
    12361 atcccagcgg cctcttccac ttggtcccag ggcatcggcg cggagtgtgc cgacagtgta
    12421 gcgaccgagt cgggaccggt tttcgccgac gcgtccatcg tgggggtgga cacctcggca
    12481 accgatgcag aagctatttc ggccggctct atcgaagcag gatcgacctc taagacagga
    12541 ggatcacttt caccggcagc ctgcaccgac tccaccgccg aggtaacggc tgaaactgaa
    12601 gactcagatt gaggttgaac ttccgactcc tgtggcgaat cgacgggcag cgtaaccgac
    12661 agtgactcct ctggcttggc ctgcagcaga tcttctgtca ccgcctccag cgtcgaggag
    12721 gtggtggtga aagcaatcac ctcaggggac tgctcgaccg ccgagacctg aatttcctgt
    12781 tggcgtggga catcactgga ggccgtctcc atttgctccc aaggcatgac caccgcagag
    12841 tctgtaacag ggggagtctc gatcagtgcc ggggcgtgag cggtctcgtt gatagaatcg
    12901 accacaggag gctggggagt aacctgcagg acctgcccgg ttaccgtgtc gaacatggcc
    12961 gcgaaacgaa aggccaccgg acttcctggg gctagggaac gaatccgcac aaagagatcg
    13021 ctggcccggt tcggatggtc ggtgtccgga tcttccagca acacctccac cgccttgccg
    13081 tactcagcca ccgccgccat gacgtcgccc ttttcctcgt agacacggcc cagcatctcc
    13141 aggaaaggaa catagcgggg tcccgccgtc agatattccc gcaagagcga ctccgccaac
    13201 caatagtcct gccgctccat cgcttcccga gccagcgatt cgaacgccgt cctggcgctc
    13261 atgagactgc cgagccgcag atggagagtg gcgaagaggc gccgggcttc cgcgttgagc
    13321 gggtcaagcg cgagcaggtc tttcaacgcg gaggacgccc ggccgtactt gccggcgttc
    13381 atttgtgtaa tggcatcttc gagaagcgcc gactttttgt taaagccgat gctccccttt
    13441 ttctggacct tcggagggac ttttttgtct gacttgagag gagatttctt atcagatcgc
    13501 actgtataaa cctataaagt tacagacatt tctcgcgccg catgactcca tgactagaat
    13561 gccagcaggg actatcccaa ccgggcaatc caccgccttg aacaactgca ccattcgggg
    13621 gaaagcaagg gaagtgcctt ggccactaca tggtcctctt ctgagccatg atgcggtgat
    13681 gaccgtggaa tgcctgccgt ttacctggat aacgtcaccc agctctccga cacagccatg
    13741 tgtctatcct tttcggcatt accgggcacg atcttgatta gggattctcc tgtgccggtt
    13801 ctgacacaca gtctggaagt cgccaccgtc ggcagacggg agcactgact gtccgagaat
    13861 gatgtgttat ccgtctccgg gggttcgtat actgtgatca aggaggactc cttgaccaag
    13921 gcccacatcc tcatccttac ggacctcgac ggaaccctgc tcgacgccac cacctattcc
    13981 tacgaggcag ccacgggcgc cctctctgcc attcagaccc tgggtgcaac gcttatcctg
    14041 gtttcgagca agacccgatc ggaaatggag ccgctgcgac ttcgcctgga caatcgccat
    14101 ccctttatcg tcgaaaacgg cggagcgttg ttcatcccca tgggctattt cccatttcct
    14161 ttggagcagg ccgcaccccg cggggactac gaggtggtcg aaatcggcac cccctatgtc
    14221 cggctcagga cggccctgaa agaaatcggg cgggatctgg gctgtcgctt gcgagggttc
    14281 ggggatctct ctctcgaaga ggtgtcagac ctcacggggc tgtctcctgc ggagactctg
    14341 ctggccaccc aacgggaata cgatgagccg gttgttgtgg aatcgaatgg gatggtctgg
    14401 aaccgcttgt cagcagcggc ggcaacacgc ggcttgcgct ggacccgagg aggccggttc
    14461 tatcatttga tgggaggaaa cgataaaggg gttgcgagcc ggcgactcat tgcctggtac
    14521 gagcggcgcg cacagcagga aggacgcgcc ctgatcaccg tgggcattgg ggatagtctg
    14581 aacgaccttc ccatgctgga agcagtgacc taccccatcc tcgtccggaa gccggacggt
    14641 tcctacgatt cggacgtgca gtttccccac ctcacccgcg ccgaaggggt ggggcctgtc
    14701 ggatggaacc gaagcctcat ggatctgttg ccgacccttt gacgatcagc ggatcggcac
    14761 tcccgaacca cccggcacca acgctgcaat ggtcgactca tggttactcc ttcgcagaga
    14821 caggaggacg acgccgacac tggagacctg ccagaagccc accggcaaac cctagtccga
    14881 ccgcaaacgc cacaaggccc acaagcgagc ggttgaacac gaacgggctc tggcgccggt
    14941 gcgggtaacg gggcacggcc gcttcgccgc ccagccagcg ccgcgccttg gcctcagcat
    15001 cgacatctga aattttatcg gtcacgctag atccttacca gctatatgtt aggtgtcgaa
    15061 acagcggttg cctagagatg gcgcaagtca tcaggcgccc cgtcccgcgt gcggatcgaa
    15121 agcccgcgct caagatagtc tttggccaaa tccttggcgc ccaggccgaa tgcaatcgcc
    15181 ccggccatga cgagcccacc gagtgtaatg ccgaatccca ccaccacgat attctgggcg
    15241 atcccgagtt gctcaagcgc catggcgacc gccaacagtt gaatccccca tcgcgagcag
    15301 gtcgccacca gtcgcgccgg aggcaaccct gcattgacgg cggcgatcag aaccgcctgg
    15361 gacacaaagt tggataataa ccaaccggcg acgagaatga gacccgccgt cacgagatgc
    15421 ggcacatacg ccaggaacga cttggcgaaa tcgttgatcg gctggagatt gagcgcaccc
    15481 aaggcggcga cggtcgcgaa aatcataacc aaccaatagg ccgcttgccc caccagacgt
    15541 gacggatcgg ttttgacccc tccgcgcagc aatgcgcccg tcactcccag tcgatcgaac
    15601 aatcgatcca gcccgaccac ccgcagcaac cgctccagcg cctgactggc ggtccacgcg
    15661 accaccaatc ccgcgaagaa aatgatcgac atcgccaaca ggctcggtaa caccgcaagc
    15721 acctgccgcg ccatcaactc caggggatcg agtagcccct tctcaaaaaa ttctttcata
    15781 cttccctcct ccggcctcta ctgcagcctg ccttcagatg gatcgagttg cgcgcattac
    15841 cctgcatgtg gctcattcca gcgcgccacc agataggact tatccttttc aaacgcctgg
    15901 cacaaggctt cgatgcggcc ctcggcctcg ctcgatgtga gcccctgggt atccaacaca
    15961 aacgaagcgg ttcgccccag gtacagggga gtgaaggcct tcaagacatg gtcgcgcggc
    16021 aacacgcgat tgcggtaggc cacggcggcg tcatacacga cccgtgccca cagattgtcg
    16081 gcaatacgaa agtcttgcgc cgaacaggtt cccaacggcc tcagactctg tctggtgccg
    16141 ggagcgagaa tctgatccca aatcgtcccc agatcactca ggccctgtcg aaaggtgctg
    16201 atcatccgat cgacattgac gttgaccggc tcgaccccga cctcgtattg aaacccgaac
    16261 aacggtacgg cattcgagcc atccgtcttg ccccacaccg catagtgatc ttccatcagc
    16321 gcgaacacgg cgcccagcac ctgcaccaac atcgccgaca ggtccgccgc cggatctttg
    16381 gggttgtgga tcttcgcgcc cagaaagctt tgacagacgc gcgctccgct ggcgatcgct
    16441 tccgtcgtca tccagatgtc gatgccgaac ttggccactt ccgattccca cacatgcttg
    16501 tcgagatagt gctgggccaa ctggccggaa aaaccgaaat cgccgccgat gggctgtctg
    16561 atacgatgac catagagggt tcgcgtcagc ggatacacaa tgctgttggt gatagtcccg
    16621 tcatacttgt gccgcagata atagggcgcg acgtagtcga acccctgctc aaacaccggg
    16681 ctcaccagca actcaatcca ttccggggtg atgctccgca gatccgagtc cacgacggca
    16741 caggccttgg ctttcaagcg acgcgcaatt tcaaaaatcg tgcgaaaggc gctcccctta
    16801 ccgggaatgc cgtgatacgg cgtgatgatc ttgtggagcg agctctgctg atcgccgatg
    16861 aagagggtct tgagatcgac gacggcctgc gaaaccacct gctgcgtccc gtcggtcgag
    16921 ccgccgtccg aattcaccaa tacggcgcgg gcttccggaa aatacttggc caggccggcg
    16981 ctcaccgcct tgacgacatg cccgatggtt tcgacattgt tgaagctggg aatcccgacc
    17041 aaaatatccg tatgggcgaa ttggtcgacc tgcgcctccg tttcgggcgt cagtggggtg
    17101 gtatgggtcg catcgggaag ttgggggcca ggcgtcatac gtcaagtctc gcacgcctcc
    17161 gccctggcaa accatctatg acatgaccga gctggaccgg cgaaaggctg gtgtgtgtaa
    17221 caacgtgtct tcgctaagag acaccatctt gcccgatgat gtcaagcaat cctgccgcca
    17281 cgccgcttga ccaccttccg catctcgctc acacatttca ccaacacagc agcccgatag
    17341 gcggcagacc tcgatgacac aaggcgcatg tacgcagaat cacgagccgg caggtaagga
    17401 aaaagagcga ggacaagcgg aggaacattc gaatcggcta tccggcgtcg gaaatgacga
    17461 tctgttcttt ccgatccgta ctgaacggag gagtggtgtc atgggcgctg acgaagtcgt
    17521 tcaaacggag ttggtcatcg gcatccataa gaatgggatc gacccccagc ttcgactcgc
    17581 tggcccagcg gatcgtcgcc agcctcaccg agaggggcct gggctgcccc ggaatatgca
    17641 aatgcagttg gagtaagtct cccgcttgca atcctttgag tccagtccgt gaccatcccg
    17701 gaatcgagag atcgagcatc cggccgttcc acaccaacaa accatcttcg gagcgtgatc
    17761 cggtacactc gacgataaaa cgcgggcgat tctgttgttc catccattcc tccaagcaaa
    17821 cgcctcggca gaatatgaaa aacaacgaat agcctgatag tccccgaaag gtgggggcgc
    17881 tccggcccga tcgtttttat cctgctcaga gaggttcgta gtatcgccgg cgaaacggaa
    17941 ggaagaggga acggaaacga gagctgttgc tgcgaaccgg acccagacta ggtcggagag
    18001 ccgaataacc gataaacgat taaggagtca cgcaactcgt gaagatcccg actccttgag
    18061 cactgactcc acaaatttgt gaatgcgtgc ttgggcggta gcgctcatcc tgacggtttc
    18121 aagcccaaag acacggcctt gcacccactg aaccgtcacc ccatctacat caatcgcctt
    18181 cggctggtca ggcaaaaaga cgcgcaacgt gagtttcatg ccgaccgtga ccggcttatc
    18241 cccgtcgatc cgccagccct tgaccgaaag atcgcacacc ttgccggttc cgaccaggcc
    18301 ctccccgaga taatagagga agcagctgac gggcacacgg gaatgggaac gagatgtctg
    18361 tgacttagcc atgatttccg gcgagggaga gggctcgatg ccgtctcagc ctcgcgacga
    18421 ctcagtctac tcaactcgct ggggatggcc aagaatattc agtttctcgt cagggcaggc
    18481 agtcccgctt cgcaatctca tagatcggaa aaagataaat ggagaagggc agcggtcaga
    18541 ctaaggccgt gacgaactgt tcgaggcgag catgctcatc ggccttgatc tcattcacct
    18601 caatcccgaa ttcctgccca cgcgaccacc gcacgactgc gcggtctata aacacggtgg
    18661 gatgctggtc gggaagaaac acgcagaggg tcacgaccgt tccgggctcg accggatgtg
    18721 tcccgtcgat acgccaccca ttgagggaga ggttccaggc gacgccggtt ccctggagcg
    18781 catcgctgga gtaatacaca gaacactgaa cgggaaaacg acgaaaggac cgcaactgaa
    18841 atgtcgtggc gaccggagcc ggcgaggagc gcggggccaa tggagcgacc tgtttgttgc
    18901 caccggtcat ttggctaccc gctcgagtga tccgtatccg cagacgttgg ggtgagtgac
    18961 gggtttcgaa cccgcgacct ccggatccac aatccggcgc tctaaccagc tgagctacac
    19021 ccaccacaca aggtcaacga ggaacgcgca tcttaacaaa ccccgaagaa cagtcgcaac
    19081 cgccccgaga cctcaccctg actcagcttt tgctgcgtag aatggccggg cgaagtcgcg
    19141 acgcattctg tatccccggt cgcatggacg aaatcaactt gggacttgac cgtcaacgcc
    19201 cccggcgatc gaaggccgct tgcatttctt ccaggatggc cgccgtggcg gcaggaggat
    19261 cctgagcatc gcgaatagga cgaccgatca ccagatagtc ggccccggct gcaatggtct
    19321 gggtcggcgt cgtcacccgc gcatgatcat ccgtcccttt tcctgccggc ctgaccccgg
    19381 gggtcacgat aaccagattc ggtcccacct tggcacgaat cgcctgcggc tcttctcccg
    19441 acgccaccac gccgtcgcag cccacttcgg ccgcgagcat ggcccgcgcc gttaccaaat
    19501 cctgcacgct tcgctgaatg cccatctccc gcaaatcttc cccgtcgaaa ttggtcagca
    19561 ccgtcaccgc cagcagtttg agatcggacg atccccgtcc ctgcaccgcc gcaatcaacg
    19621 cttttcgatt cgcatggacg gtcagaaagg tggcacccat cgcggcgacc cgggccgtcg
    19681 cacgccggac ggtttcttca atatcgagga acttgagatc aaggaacacc cgcttcccac
    19741 ggtccaccag cttccggacg atgtccggcc ccgccaccgt gtacaactcc aggccgactt
    19801 tcacaaatcg cacctggtct cccatgcgag ccaccagcgc atccgcttcc gccgcggagg
    19861 gaacatccag cgcgacaatc aaccgatctc gtgcatcaat ccgtgtcatc atgtgtacgc
    19921 cgttaccccg ggttgcctct gggctcggct gactcctttc agtgtccttg acctctcttc
    19981 tcatcctatg ctacaagctc aagctttgaa cgtaggagaa tccgtatgcc tgttgtccac
    20041 aaatccacga ttcgccgtgc ccgtcaatct gagaagcgtc gtttgcgcaa ccgctccacc
    20101 atcagctcgg tccggagcat cctccgcaag gttcaggatg cgatcacggc caagaagccg
    20161 gatgaagcca aggccaccct gcgggaagcc acttcagcgt tgagcaaagc ggtcaccaaa
    20221 ggtgtcttga agcccaacac ggcctcgcgc cgagtctccc ggctcaccgt gcgtgtgaac
    20281 gcaatcaccg cttccgcgta acggttctga cattcgacac cggtctcgaa cccgacgggc
    20341 gcgggaccgg tggtgccggc tgcacccgcg ctgcggatga tgctgatttc gacggctggc
    20401 cacagagtcg gaacaagagg tcttccatca cccgaacagg ggcgcttcca cttccgcctt
    20461 tcagcttcga gtcggtttcc aaaaaccagc gaaacgtgtg cgtcagttgc acatctgaga
    20521 attgcgccag aaacccgcgc acccgggatg ggtccatccg taacgtcctg gccgcttccc
    20581 cttctcgacc gccggctttg agctgctctt tcattttcca cagccgtcgg tattgccaga
    20641 ccaaggcacc gagaatccgg agcggagcct ctccattctc aagatttcgc gcaaggatcc
    20701 gtaacgcccg tccatgatcg tgcgcaccga tggcgttcat gagatcgaag acggatgcac
    20761 cggactccgt ccctctcaac gcctcgacat ccgccggttg cacggttcgg tctggcggca
    20821 cataggcggc gagcttctct aactcccgct tcaccgcgta gagcgactcg ctccccacct
    20881 ccttgagcaa ttgcgtcgcc tcctcgtgga tgcgcacacc cagcgccgcc gcttcctggc
    20941 gaatccacgc cgacagctgc gcatcgcgca gcggggcgca attgaccgtc actgcccctt
    21001 tcgtcaaggc ttgggtccat ttcatccgtc catccagctt gacacccacc acgatcaacg
    21061 tcgttgtgtc attcggagcg gagaaatagg agaggagttt ttccccttct cgagccggga
    21121 gtttttccac cgacttatag accaccacgc ggcgttcggc gaacaccgga atttcggccg
    21181 cgcaggccaa cacatcctca accgagcagt cgtcgccgtg aaaaatatcg taattgaatc
    21241 cggtatccgc cgccgggccc aaggctgcgg cgcgcaacgc ggccacggac tggtcccgga
    21301 gatagtcttc ttcgccgacc acggcataca gcggcatcac cccgctgcgc gcgagggatt
    21361 ggggcaattc cagactcatc actgaagcgc tcatccgcag aaactctcca tcaattatcg
    21421 ggccccataa ggcggtgtcc cgccggccgg agcgacctca gctttcaccg gacgcttcag
    21481 tgccttgtcc aactcgcccg tatccagaaa gagcaggaat cgtgaggcca ggtcttccgc
    21541 gacgaaccgg cctgcttgct ccaacgcccg attttgcagc acccgattga attgcagatc
    21601 ctgggtcaga aaaaactcag aggttccctt cgcgacctgg taccagacga cctttttcgt
    21661 tttggcctct tcgatcctca cgctcacgag catttccacc cggctctcaa atgtcgtggt
    21721 ctggtcgaaa ctcaaggtcg gcagcgtgat ctgcatgatc tgtccggaca gatagagatc
    21781 ggccccggcc gaagggccca cgatttccgc cccggcccca gccgagaact cccgacgcag
    21841 gtaattggcg agcttgacct cgaagttcgc ctcgtaggta ctgttggcaa taggaaggat
    21901 ggccaggcgt ggcgcgtgcg gccgcgtcgt cgccgcctcc ggcgcaccgc cgaccgtggg
    21961 accggcgccc tgcacacgaa actgatagcc acagccgggg agcgagacac tcaacgcaaa
    22021 gaaaataatc agccacacca cgcgaccagg aacctccggg ccggcacgtg ctgcgttgtt
    22081 cctgtaacgt tcaggctggc gcacggcatc acaccacgaa gttgatcaat ttcttctcga
    22141 cgtaaatgac cttcttcggc tctttcccct gcagccattc agccacttct ttcttagcca
    22201 gaggctccaa ctgctcgcgt gaagccccgg catcgacctc gagttttccc cggagctttc
    22261 cgttcacctg aatcgggatc gtcagccggt cgctgaccac caacgcggga tcgaactgcg
    22321 gccatggctg ctggccggcg ctcggttgtt gaccgagacg ttcccacaac tcctcactca
    22381 gatgcggcgc aaacggcaag agcaacaaca caaacggttc cagcaggcgg cgaggccgct
    22441 gctccagttt cgtcatctcg ttcgtgaaca ccatcatctg cgagatcgcc gtattgaagc
    22501 gtaaggcgtc gatatcgtcg gtgactttct taatggtgta atggagcagc cgcagctgtt
    22561 caggcgtcgg ctcggtatcc gtaaccgccg gactcaatgc gccctcgtcg ccgatcatca
    22621 gccgccagac ccgatcgaga aagcgcgtga tcccctccac gccgcgggta ctccaaggct
    22681 tcatcgattc gagcggaccc atgaacattt catagagccg caccgcgtcg gccccgaact
    22741 ggtcgaccat ctcgtccggg ttcaccacat tgccgcgcga cttggacatc ttctgattgt
    22801 cttcccccaa cacaatgccc tggtgcacca gtttcttgaa cggttccggc gtactgacca
    22861 ccccggcgtc gaacaagacc ttgtgccaga accggctata caacaggtgc agcaccgcat
    22921 gttcactgcc gccgatgtag aggtccaccg gcatccaata gcgctccttc gccggatcga
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgtctccagc cagtcgtgga
    23101 gattcgccaa ggggctttcg ccgctgccgg agggtttgaa attcttggtc tcaggcaatg
    23161 ggagcggcaa ttgctcctcg ggcaagggac ggggctcgcc gtcgacccat acaatcggaa
    23221 agggctcgcc ccaataacgc tgccgggcaa agagccagtc acggagtttg taattgatcg
    23281 tcttcttacc cctgccctga gtttcgagcc aggcggtcat ttttggaatc gcttcgctgg
    23341 gcaatagtcc atcgagagag caggatccat cgggcgtggt cgagttgatg acacgtcccc
    23401 gatccgtctc gacgaacgcc gccttatcga cctgcccacc ctcaatcacc tctcggatcg
    23461 gcaggtggta ttgcgtcgcg aaggcccagt cacgctcgtc atgtgccggc accgccataa
    23521 tcgcgccggt gccgtaactc atgaggacat agtcagcgat ccagatcggc aggcgcttct
    23581 ggttcaccgg attgacggca tacccgccgg caaagacacc ggtcttgacc ttgtccagtt
    23641 cctgacgctg tagatcgctc ttgcgggacg ccgcatcgcg gtaggccgtc acctgcgcac
    23701 gctgctccgg cgtcgtcacc atgtcgacga gcggatgttc cggcgccaat accatatagg
    23761 tcgcaccgaa gagcgtgtcg gggcgggtcg taaacacgcg caaattgccc gtcttgtcgg
    23821 ccaagggaaa atcgacttcc gcgccgatcg accggccgat ccaattcttc tgcatctcga
    23881 gcgtgctggt cggccattcg accagggtca ggtcctccag caatcgctcc gcataggcgg
    23941 tgatcttcaa gacccactgc cgcatcggct tgcgaatgac gtcgaacccg ccgacctcgc
    24001 ttttgccgtc cacgatttct tcgttggcca gcacggtccc caaggcagga caccagttca
    24061 ccggcacttc ggccacgtag gcaaggcccc gttcgaacag cttcagaaag atccactggg
    24121 tccaacgata gtagtcctgg tcggtcgtgc tgagctcgcg ttcccaatcg taggagagcc
    24181 cgacgcgctt catttgccgt ttaaaggtcg caatgttctg cgcagtggtg atggcaggat
    24241 gcacgccggt tttcaccgca tactgttccg cgggcaaccc gaacgcatcc caccccatgg
    24301 ggtgcaacac gttgaagccg cgcatccgtt tatagcggga tacgatatcc gtagcggtat
    24361 acccttccaa gtgcccgacg tgcagacccg acccggacgg gtatgggaac atatcgaggc
    24421 aatagaactt gggcttggaa tgatcgtcca ccgcccgaaa cgggcgatgc gtctcccaat
    24481 aggtctgcca cttcgattcg agggcctgat gatcgtacgt cttgctcatt gtggtatcgt
    24541 catacgcagg gcaaccagtc ctgtagaatg aaagaacgga cgactctagc acacgccgca
    24601 cggagcgaca aggacgaggc ggcacaaaca gggaccgagt cgaccgcgaa ggatcccccc
    24661 tcccttgatc ccggaatcaa gtcctgttca aatgccggga caaccaatcg ccgaagatcg
    24721 accgcgcacg acctcacaga cttttgccaa cgccgatgta cgctacctac atctttccgc
    24781 gactcatgga ctgggtgctc cggggcgaac ggtttcagac cgaacgccgg ctcctgctga
    24841 cgccggtaca tggagtcgtc ctggaaatcg ggttcggaac cggactgaac ctgccgcact
    24901 accccacgac cgtgactgct ctgcacaccg tcgatcccgc cccgctcttg cccgatcgag
    24961 tcgccgtacg ggtggcacag gcttcgttcc ccgttcacat tcagcatgtc agtgccgaac
    25021 gattgcccta tgacgatgcc agcttcgact acgcggtcag caccttcacc ctctgcacga
    25081 ttccggatcc ggcgagggcg ctacgcgaca ttcgccgggt gctgaagccg gatggccact
    25141 tcctgttcct ggagcatggc cgcagcgacg atcccctcat cgcccggtgg caggatcgac
    25201 tcaacccgct gcaacacctc ctggcctgcg gctgcaatct caatcgccgc atcgatcaac
    25261 tggtactgga cgcaggtctc cggctggaac aacttgatcg ttattgtctc cccggcgttc
    25321 cgagaatcgg cggggaaatg tatcgcggca ccgcccgcct caacggacaa tcgtccctgc
    25381 attccagcct cgacacctaa cacactcgtc acgaccttgg cctcgcaccg cgttcggaac
    25441 cagccaaagc agtgacgaca tgaaacagga aaattgtatc cctatcggca attttgtaga
    25501 ctgcaccgta taatgcacga tcctcgcatt cgtccatgca acagacggga gtgacccgga
    25561 tgtcctcctc acagtcgttc cccgacagaa agcctccgtc gacctctcca ttattcaaac
    25621 ccatagattc agcccgggac agcagcatcg ccttgcaacg ggtgacgctc ctttatgagg
    25681 cgatgccggc cgcgctggtg gccggactcg gctgcgcact cgccctgatc gcggtcaatt
    25741 ggcatgtcgt cccgcacgac cggctgctcg tctggctggt gtaccatctg atcctcgcgg
    25801 caggccgata cgggctcgcc cgaacgttca cggccgacag gccgggcccc gacaacatcg
    25861 agcgctggga ccgggcattt ctggtcggca cgacactcgc gggtctcggc tggggcgcct
    25921 cagccctgtt gctgtttccg gaatcctcac cggcccacca ggtttttctg gccttcgtgc
    25981 tcgcaggcat tacagccggc gccgcttcca ccctagccgc tcgcttcgac gcctttctgt
    26041 cgttctctct tccgatgttg acacccctca tcctgcgact cttcgctctc gaccatgagc
    26101 aagccctgcc gatggcgatc tgcaccatgc tcttctcgct gctcatggtc tacacggcgt
    26161 accggacgtc caacaccgtc ctggaaaccc tccggctgaa atatcacaac gcccaactgg
    26221 tggatcaact gaccgggcaa ctgcaggaag gggagcagat ggaactgctc ctcaccgtca
    26281 agaccgaaca ccatcgcttt atcatggagt atgcccagga catcatctat cggacagacc
    26341 ggagcggccg tttcacgttc atcaatcccg cggtcatccg gctcctggga tatcacgaga
    26401 cggagatgct cggccatcgt gcgctggatt tcgttcatcc cgatcaccgc cgcccgaccg
    26461 aacgtttcta tctccggcaa tttctccgga agacggccag cacctattgt gaattccccc
    26521 tgatcaccca cacgcaacag accctgtggg tcggacagaa tgtgcaacta ctgcaacgcg
    26581 accaggaggt catcgggttc caggccgtgg tacgcgatat ttccatccgg aaacagaccg
    26641 aagaagcgtt acgcgtcagc caggagcgat accgcgcgct gttcgagagc agccctgaaa
    26701 tgttgctgac cgtcgacgcc cggggcacct tgctgacggt caataccacc accgccgccg
    26761 aattgggcta cgaggccgac gagctgatcg gtcaaccggt agcgcgaatc gttcacccgg
    26821 atgaccacgg cagcatggac aagcattttg ccgactctct ccgccggccg ggagaagtcc
    26881 tccgctggaa tttccggaag atgcgcaagg acgggagcct gctctgggtg cgagaagccg
    26941 cccgggccgt gcgtaactcc gacggacagt tcgatatcat catcgtgtgc gaaaacgtga
    27001 ccgaacagaa aggcatcgaa gatgccctga cccagacgcg ccaactcctg gagtccatcg
    27061 tcgagcacat tccccacatg gtctttttaa aagacgctca agaattgcgc ttcgtccagt
    27121 tcaataaagc gggcgaagac ctgatcggcc tgccgcgaga ggcgctgctg ggaaagaacg
    27181 attttgattt cttcccgctc gagcaggcgg aattctttac cgccagagac cgcgaggtcc
    27241 tgaccggcgg cacgatgatg gacattccgg ccgaaccgat ccagaccaag gatcatggcc
    27301 tccggtggct ccacaccaag aagcttcccc tctacgacca ggccggcatg ccacgctatc
    27361 tattggggat atcggaagac atcacggacc ggaaacaacg cgaagaagcc gaacggctgc
    27421 gcttggggaa attagaaaca caacaagccg tgctgcatga attggcggaa gatccggcca
    27481 tccatagcgg acacccgcag caagccttcc cggtcattac cgaatatgcg gtgagcacgt
    27541 ttgccgtcga acgagccggc atctggctct ttggcgagaa tcagtccgca ttgatcctgc
    27601 aggatctgtt cgaagccact cccaaacacc atctacaggg aaccacgctc tcgaccggtg
    27661 aatacccggc ctacctgcat gcgctcgaaa ccgaacccta ctcgctcgcg gcccatgacg
    27721 cgcagaccga tccccgcacg aaggaattga ctccgtcgta ccttaccccg cttggcatcg
    27781 tggccatgtt ggacgccccg atccgcaggc atggtcgcgt agtcggcgtg ttatgcctcg
    27841 aacacatcgg tccggccaga gtctggacca cagaggaaca gacgttcgcg gcctccctgg
    27901 ccgccatggc gaccctgacc ctggaggcgt cggaccgtcg tcaagcccaa gaggcgctgg
    27961 agcaacatgt ccgtgaacgc accggagagt tgcgccggac gaccgcccat cttcagacca
    28021 tcatcgaaga gtcccccctg gcgatgatcg aattggacca agcgggcctc gtcaccacct
    28081 ggaacgccgc cgccaccgcc ctcttcggct ggaccaaaga cgaggtgctg ggcaaggagc
    28141 tcccttatgt gcctcccgga caggaaaagg tgtcggaaga attgtggacg gcggtgatga
    28201 gcggcgccgc gccgcgcaat ctggaactgt gccgccagaa aaaggacgga gccctggtgg
    28261 atgtgaacat gtggggcagc ctacttcaga gtccgaccgg ccaggcggtc ggctccatcg
    28321 gattcttcgt cgatatcacg aagcacaaac aactcgaaga tcaactccgg caggcccaca
    28381 agatggaagg gatcggccgc ttggccggcg gcgtggccca cgatttcaac aacctgctca
    28441 ccgtcatcaa cgggtgcgcc acgctcgtgt tggaccaggt gcgggccgag gatccgcttc
    28501 gtcgttcgct gacggagatc ctgaccgccg gccagcgggc ggcggccttg accaggcaac
    28561 tcctggcctt cagccggcgc caagtgttga cgttccaggt ctttgacctc aacgaggcgc
    28621 tttcgtcgat cagctccatg atcgaacggt tgatcggcga agacatcaac ctgaccagaa
    28681 atcttgactc gcggccctgt accattcgcg ccgacagagg acagatcgat caagtggtac
    28741 tgaatctggc cgtgaacgcc aaagatgcca tgcctacagg cggcacactc acgatggcga
    28801 cgcaggccct gctgattaca ccggacagcc ccgtgcccca tcccgcatta ctcccgggag
    28861 cctatgcgca cctgtcgatc cgcgactccg ggcagggcat ggaccgcacg accctgtctc
    28921 atattttcga acccttcttc accaccaaag aagaaggcaa aggaaccgga ctcggattgg
    28981 ccacggtcta cggcatcgtc aaacaaagcc aggggttcat ctttgccgac agcacacccg
    29041 gcgcggggtc aaccttcgac ctctactttc cactggtgga ggcgccgccg ctcgctgccg
    29101 cgccgacact tccatcccgt ccacactgcg ggctggaaac catcctgctg gtcgaagatc
    29161 agcatgccgt ccggttgctg ctcacccagg ccctgtccga ttacggctac accctgctgg
    29221 aagcctccag cggccaggaa gcgctccgcc tcgtcgccgc cgcccgcacc cccattcata
    29281 ttctgctcac ggacgtcgtc atgccgcaga tgacaggacc ggccttggcc gagcacctgc
    29341 gtcggcaatg gcccgagtta cgagtgctct ttatgtcggg atatgccgag gggagcgtgt
    29401 taccgacatt tctcgcgcaa ccgggcaccg gcttcatcca gaagcccttc ctccccactg
    29461 aattggcgca gaagctccgg gaattgctcg atcccgccaa ataggacacg tgatggattg
    29521 tgcaaaaaaa ccggatgcgc gacaaacggc ttagccgggc aggatcgcgt ggaaatgttc
    29581 gatggaggta aaccgcgcgg aaggaacggc ggaagcctga gaactcgacc tggagcggtg
    29641 aaaaatccgg ccgatcccga aatcttccgc cgcagccaga cattgctcat cgtcatcgat
    29701 gtagagcgcc cgcgcaggat cgaatccgac caattgccgg caggtcggcc aatactccga
    29761 tcgcatcttg agccacccca cctcgaaggc atccacgata cgatcgacat gccgatccag
    29821 ccccgtcttg gcggttttca catccactcc tgcccgatgc gcgttcgtca ggatcgtcac
    29881 gcgcttcccc aatcgacgca aggatgagag aaactcctcg gcatcgggaa gaaacccgat
    29941 catgtggtcg agttcgcgat gcatcgccac cacatcgatg tctacccgct ccgaccaata
    30001 atgtaaatcc gtccaggcca attccccttc gacggaacga tacatggcca tcaagcgatc
    30061 ccgggcggcc tcgaaggtca gcgcatgctt cgccgcatac cgcctcggca gttcttcctc
    30121 gaagaaaaag ttgtcgaaat gacggtcgag taacgtgccg tccatgtcga gcaacacatc
    30181 gtcgatccga ctccaatcga cggactgatg agcggcctct gagcgatttg acagcatcct
    30241 ggcagtatac cggagaccgg cgcggcaaga cgagtcattg tgttcggtga aatctctatg
    30301 ctaccgtcgc gcaccatgtc acgcaccaaa caaacgcgcg gtcctgtggc cccgctcttc
    30361 acccttgagg gcggccggtt gcccattatc gccctcatcg ggcggcccaa tgtcgggaaa
    30421 tcgacgctgt tcaaccgtat tctcggcacg cgggcggcca tcgtggacga tgtgccgggt
    30481 gtgacgaggg atcgcaacta cgccgattcg acctatcgca atcgccgctt ccgcctggtg
    30541 gatacgggag ggctggaccc cacagccagc gacggcatgt tgtccctgat ccggcaacaa
    30601 tcccaactcg ccatcgccga ggcggatatc ctggtcctgg ttctggatgc ccgctcaggc
    30661 ctgacgccgg ccgacgaaga agtcgtacaa accctgcgcg gctccgataa acccgtctac
    30721 tacgtgatca acaaaatcga taccccgaag gccgatccgc tggtcgccga tttctatcgc
    30781 ctcgggcagg agcagctgta tcccctgtcg gccgaacatg gcatcggcgt cgccgagttg
    30841 ctggacgatc tcttctcgca catggccgag cccctcgacg aagaagccac gagcgacacg
    30901 ccccgcatcg ccatcgtcgg acgtccgaat gtcggcaaat ccaccttggt caactcagtc
    30961 ctcggcgaag cgcgcgtcgt cgtcagcgat gtgccgggca cgacgcgtga tcccgtcgac
    31021 tcggtcgcga cattcaaaga ccggcagtac gtgctgaccg atacggccgg catccgccgc
    31081 cgtggtcgtg tggaacgagg catcgagggc tacagcgtcg cccgatccct gcgcgccatc
    31141 ggccgatcag acatcgccgt gttgctgctg gatgcggaag aaggcgtcac cgagcaggac
    31201 actaaaattg cgggcctcgt cctcaaacaa ggacgcgcct gcattctcat cgttaacaag
    31261 tgggatctca agaccgacga cgtgcacgct cgcgaggcct acaaacaaga tcttgaacgt
    31321 cgcttcccat tcctcgcctg ggcaccggtg ctgttcattt cggcgctgga gcaggatttt
    31381 ctgcgcggct tgttcgcctt gatcgaccag gtctatttct cattctgtaa acgggttccc
    31441 accggccccc tgaatcaatt ctttcagggt ctgctggaag aacatccgtt gccggtcagg
    31501 aaaggaaagc cggccaaagc cagcaaatcg gcattcatga cgcaagttgc gacacggcct
    31561 cccgcattcg ccctctttgt gggacatccc gacaatatga ccccggccta cctccgtttc
    31621 ctcgaaaacc agatcaggaa ggaatatcat ttctccggca cgcccgttcg actgttgacc
    31681 agaaaaaaat gaggcagcct tgccggagcg cgacgcattc gacacaatcg acgacatttt
    31741 ccttcgcccc cttcgtttca ccgctcacga ttccttgaac atttcgtaga tttcctccct
    31801 tctctcagct caacgcaatg gcccgcatct tgcttttcca ttcagcagtc gacgctcagc
    31861 gacaaaccaa cgcgctgacc cgacggtgga cagccatagc agagaggagc ctatctctat
    31921 gttcatcggg caaccagacc tcgccgcccc cgctgcaccg acctcgtcgg tcgagaccgg
    31981 cctcatcgtc ctttccatgg acggtcgcat tctccatacc aacgaccggg cacgcgagtt
    32041 cgcgcgccat ttcacgaacg agccgcgccg gctgcaggcc cccgcgacca caccactccg
    32101 tcatccgttg atggacctgt ttcatgacgt cctggccaat ttagagaaac acatcgcggc
    32161 agaagactgg agccaattcg aaatcacccg cctcgcccga tggggcggcg gcacggtgcg
    32221 tctccgggga ttcggcattc cagaccaagc cagacgccag cagtcacgcg tcgtcctgac
    32281 agtccattac cagtcgagcc cctccccttg gtaagattca gcctgcgcca tgcacggccc
    32341 aggcgattcg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaccaaaat
    32461 cgccggacgc caggatcacc aaccattgac catcgaggtc gatgggaccg gaaccatcaa
    32521 aggtctgcac gatgtgcagt tcgacctcca ggtcaatcag gttccccgca caccttcgtc
    32581 gctgcatctc ttgttgaacg gcacggtctc gaaactgaag tcgaactatg tgttcctgaa
    32641 aacccccctg ggcatcgtga cagtcagcgg aaagaccggt gtacggaacg ccaaggtcgg
    32701 gcaggagatg tcggtgtgga tccaggatcg acatgtggcc atcgatctgt atcaagacgg
    32761 tcttacaagt ccatcgcgcc gattcctcac cgggccgctg gcctacggtt ccgacgcgca
    32821 tgacaccctc accatgcagg gccccgaggg agcccagtcc attcccctca cgcaacggcc
    32881 ggctgctctc gcaaccctaa aggaagggct cccgatcacc gtcgaactta accagcaggg
    32941 tgaactcgtc gatatccgac gggtcaattg acgcccctct ttcggcgcct cgacatccgg
    33001 gccgcttctt gacagtgccc ggcgcccccc gtagactccc tgacgcgtat ggcatcacgg
    33061 aagaacagtc ggccggaaat ccccgacggg cgcggcgtct ccccctccac ctccgctcca
    33121 ttcgatcagc tgtatcgtga tcacgtcgac gtgatgtacc gctttgccta tcgcctgtgc
    33181 ggtgaagccg aggcggccaa agatctggtc caggaaacgt ttctgaatgc ctatcgcgcc
    33241 tacgaccgat ttcgtggcga cgcacaggtc tcgacctggc tctacgccat cgcctcccat
    33301 gcctgccagc gtatgcggcg caaacgcaaa ggcgaaccgg agcgcgagtt atccctgacc
    33361 gaattcgtac cgacatcgga gggcgagttt tccctgcaga tcccgatgga gggcctcagc
    33421 cctcaagagg cgcttgaaaa caaagagctg cggcagatac tcgatcgcgc catcgcaaag
    33481 ttgccgcgca aataccggat ggtgctggtg ctccgcgaca tggaaggatt gagcgccaaa
    33541 gaagtgggga gtattctggg gctcaacgaa cgggccatca aatcgaggct gcatcgagcc
    33601 cgcctctttg tccgcaagga actcagcggc aaaggattgg cgacagaatt tcacccggac
    33661 attaccaccg ggcaccgata gtttggtata gtctaagaag aagcaccgcc atggctgatc
    33721 gcgtacgcac atatccaggc acccgtaagc ccaccctcca ccagggtcac ggaaaacgaa
    33781 attgcgtcaa agtgctcgaa cggctgtctg cctatctgga tgacgagctt tccggtgacg
    33841 tctgtcagga aattcgcgcc cacctcggcg attgtccgaa ttgcgaagtg tttctcgact
    33901 ccttgcgcca aaccgtgcag ctctgccgca accgtccttc cccggcactc tccaagaacg
    33961 atcgtgcgaa cctccgccga cagattttga aggctgcggc aacggcgtaa cccgatcgtg
    34021 actggcccga tgatcacgcc ggccggggaa caaccggcag agggggcgag agacgagtcg
    34081 ttgagtgatc agcggctgtg cccgacgcaa cgatatttgc cttcgatgtc agaccgcctc
    34141 ctgctcgccc ttctattgac ctgcggtgga accggcgtcc tgctcgcgca accttcagaa
    34201 aatttcgacc acgggcaagc catctatcgt gagcattgca tggagtgcca tggcacaacc
    34261 ggcaagggcg acggggtcaa ggccccgttt ctctcccctc gaccgggcaa tctcatctcg
    34321 gcggcgacct ctgccaaaac ggatcaagag ttgctccgca cgatcgccca aggaaaaccc
    34381 cgcacggcca tgccggcctg gcagaacgtg ctgcccgccg aggatcagca ggccgttctc
    34441 caatacatcc gctccttggt ccgctttgcc cggccactca ctccccctcc accgggtccg
    34501 taatcttgca tcgccccagc acgccccttt cacctgaaaa gagccccgtg gtacagtgac
    34561 attcttttac tgccaccgga ggtcgttcgc atgattcgcg ggaacctgtt ccatcgacga
    34621 cagaccattg ccatcgtgac cctcgcgctc tcgctcggtc tctgggccgg gacagccgag
    34681 tcggcaccgg ctaccggcgg gaagggacat cgcccaaccg cctccgctaa gcaaccaccg
    34741 gcggtccaaa ccgccgtccg atatgccgaa gcccttgcgc acggcgatcg cgtcaccgcc
    34801 ggacaattgg actttgcctg ccaataccga tacgtcgttg cctctccggc gaaggtgaaa
    34861 caattcgcgc cggccaatga cacgtcctac gattcctgct ggcaaaccct gaccgatgcc
    34921 tatgcgccca tgctcaagcg gtcggacatt gccatggata tcatttggcc gagcaccggc
    34981 ccgctgatgt ttttcggtga cgatctcccg agggcgccgg cctcgacctt cgtggccgac
    35041 gtgctgggca cctcaccccc cgggagcggc cttcacgtga ccgtcgtcgg cagccgcacc
    35101 attccgtccg gttcgttccg cctgggacgc gcaggaaagg tgctcggagt tccaacgacg
    35161 ctggtccaac tgtctgtgca gtatcaggat cccctgacct cgccggtcac gtatgccccc
    35221 ggcaacgtca agtggacgaa cacgatcaag cgcccgagac tggcattgaa atccgtgact
    35281 acccagtggg tcgtcttcac cgggctgaag cagcatggat ttcccggcga taccgccgta
    35341 ttcaatctgc cggtcgcctc gcagccggaa gccccgggca tgatggcaga caagattccg
    35401 tttgccaccg aaacaagccg cgcactgcct gagtccctgg cctggtgggg accgaacgat
    35461 caacccggca cgttgacagc cgctgcggcg cgcgccgcca tctttcccga tctgcgcgac
    35521 cgggtggcct tactcaaccg cgtcttgctc atcgacccga agcagccgga tgccctcacg
    35581 gtcttgaccc gccacctgta tagcgtgttg ctgcgtgaag cccgcaatag ccatcaggtg
    35641 cccataaagg atcctgccct gggcattgtg gtggacgaat tttattggaa catttatgcg
    35701 cagggagccc gcttggatct ctcgaacggg atggaaatgg gcgggctctc acagccgacc
    35761 ccggcagact ttctctatcg tctcatcccg gcgcttcaaa cgctcgtttc ggtcagaccg
    35821 gaacaactcg acaaccggtt tcgacttgga atcgcctacc gatggaacaa cgaccagctc
    35881 cccatggtcg agaccttcga atccctggta aaggatattc cagagcaccg gagaacgccc
    35941 aagtccgagg ccctgctcca gctggcctgg tcccgcatca acaaagtgtc ctggaaccgg
    36001 atccttcacg atacggagac gcctcgggcc tatgccgatg ccgaggccgc cctggcacag
    36061 gccgaactgc cactcgataa gtttctggcc gaatatgcga tggcctacac catgatcttt
    36121 ttgccgaact acggggacaa ggccaaaatg cttcagcacc tgaccgaagc caagcggtgg
    36181 ttcgacgagg tacccggcaa gtcggatgag gtgtggcggt acttcctgca ctcggaactg
    36241 ctcaaagccg tgctggacgc cgatccgatg ttccaaccga tcctggcaac cgccgccgcc
    36301 ccccacgcct gacaacacac ggagcggggc ggtggagtca gaagtactcc accgccccgc
    36361 gcactccagc ctccgccgcc ttgtcagccc gcatacgctg tgctaaccat ggtgatcatc
    36421 atgtccacgg aggagtccat gcccgtcacg gccagaaccg ccagcgcaag tcgttgtgcc
    36481 atcgtcgcaa ccctgatcgc gtctcatttc agcctgaccg gctgctcaat tttcggtgga
    36541 agcacgcaac cactgatggt caattcagag ccacccggcg cacaggtcct catcaatggc
    36601 accgcagccg gaacgacgcc gttgcagtat caggtccccc gaagaggtga cctcaccgtc
    36661 gaagtgcaca aaacgggata ccagactcag tcccgagtga cgggccggaa actcagtagc
    36721 accgggatcg tcgatgtcat cgggggcgca ttgttccttc ttccgttgct cggactgatt
    36781 gcgccgggcg cgtgggaaca ggacccctcc actctcggca tcaccctcga acccgacaca
    36841 catcaaccgg cccaagcgcc ttagcgacgc ctgcatcaca gcacaaacac gccatttttt
    36901 caggagcaga ccatcatgat cgacgtccac gggtatgctg cactcaacgc tcaatccccg
    36961 ctcacgccgt tcagattttc acgccgcgaa gtgggcaaac aggatgtatt gatcaagatc
    37021 cagtactgcg ggatttgcca ttcggatgtg caccaggcgc gggatgaatg gggcggatca
    37081 ctctttccga tggtgcccgg ccatgaaatc gtcggcacgg tggaaaaagt cggggcctcc
    37141 gtcaaacaat tctccgtcgg tcaaaccgtg ggggtcggct gcttcgtcga ttcctgccgg
    37201 acatgccagg catgcaagaa gggcctggag caatattgcg aaggccacct cgccttcacc
    37261 tataacggta aagagcggga cggcgtcaca cccacctacg gcggatactc caccaaggtc
    37321 gtcgtcgatc aacgttatat tgtgcggatt cccaaacggt tgcgccctga agaagcggcg
    37381 ccgctcttgt gcgccggcat caccacctac tccccgttgc ggcactgggg ggtgggcaag
    37441 aaccatcggc tcgctgtggt cgggttaggc ggcctcgggc acatggccgt caaaatcggc
    37501 aaggcgctcg gcgcgcatgt caccgtcctg agccactccg acaagaaaca agccgatgcg
    37561 aagcggctgg gcgcacaggc ctactactct accgcaaaac ccgaaacgtt gacccaactc
    37621 accaaacggt tcgacttcat cctggatacc gtctcagcgc cccatgatct gaatgcctat
    37681 ctggaactgc tgaagaccga cggcaccatg attctggtcg gggttccgga taagcccgcg
    37741 cagctcggcg cctttcccct gatcatgcgc cgacggcgac tggtcggatc gttgatcggc
    37801 gggatcaagg agactcaaga gatgctcgac ttttgcggca cacacatgtt cggcgcagat
    37861 gtggaagtca ttcccatcca gcaggtgaat gccgcctacg accgtcttgt tcggggcgac
    37921 gtgcgttatc gcttcgtgat cgatatgagt tccctgacgt agcgcctggc atccgacgct
    37981 ctcagagatg agtcccaacc aggcacgggc tcgccttcta tgcgcaccca ctcaaatcgg
    38041 gtgggggcag aacctgtggt cagcgaatgc agaaactgag agcggctcac gaaagtggga
    38101 gagtcgaatg aagcggcagg ccgggcggcc gcaaggccgc ccggcgaatc caccactact
    38161 tggcaggagt gacaaacggc atgagcggca actgccgcgc atccttgtcc accttcaaca
    38221 gggcaatggc accgcgaagg gcgtcggtca aggagtgggt gacgatcggg tacaccccgt
    38281 cgttgttgtc cagcaccata tcgaggatcg ccgcgcccgc cggcggaatc agctgcgtct
    38341 gcacgccgcg cgtatggttg gcgggattgc cgctctccca cacatcatcc caaatttcag
    38401 cgatcggatg gaccgccgac acgttgttcg ggccggcatt cacaaaatag aaccggaccc
    38461 gttcccccgg cttggcgtcc aggaagtcgc ctcccgcctt gctgtgcacg ggatcgtacc
    38521 ggaagacgga gccgttaaac accacatgct gatacttccg gtcgaacatc ccgtcaacat
    38581 cgtcgggatc cttgaacagt tcgctctgca ccagcacata ctcgcgatcg gctttcggca
    38641 gcgccttggg atccttggga tcgacgataa tggcgcccat catgccgcgc gcaatatgct
    38701 gaatcatggg agaggcaccg cagtgatagg caaagatgcc cggccatttg gccttgaaac
    38761 gatattgcag cgactcgccg ggacgcactt ccttgtagtg ctccaggaag ttggtctccg
    38821 ccgcgtgaaa atccatggaa tgggggcgac cgttggtctc gggattcgtc agtgaaaaga
    38881 caacctcgtc accttcctgc acacgaatca ccggaccggg aaactgaccg ttaaaagtcc
    38941 aggcgtcata tgtatgtccc tccccgtcga tgacgagttt cgtttctacg gcggtcatgg
    39001 aaacttcaac gactttcgcg aacgcggatt cgaccggctg catggccagg cccgagactg
    39061 cggccaatcc caagaccgtg cgaatcacag attgacgaaa gcggatgtta cgtactgtca
    39121 taatgctgcg gctcctcctg ttgaaagtga atgggtatgg gttcattcag aaccccaggc
    39181 aggataccga atcgtcagct caaaccaata tgacaattgt catgttttta aattacctcc
    39241 ggactggtag gcgttctcct acagctcagg gtaccgctta gcgcttcgcg gccagttccg
    39301 caaaataatg cacaaacgcc ttcatcccgc ctgaggcctg gccccagtcg aaatattcgt
    39361 tcggcgcatg gtagccgtgc tcaggcaggc tcaagcccat gaacagaatc ggaactttcc
    39421 aggctttctg catcgtcacc accgcgccga tcgagccacc ttcgcgaata aaggccggtt
    39481 cttttccgaa tccggccttc acggcgcgtt tcaccgcatc gacgtatggc ccgtcaaaca
    39541 cgcctctgaa cggatgcaac atcccctcgc gctcgaccgt cacctccgga ttcaacttgg
    39601 cgacatgttt cttgagcagc gcaaaggctt tctccggagt ctgattcggt acgagccgca
    39661 tgctgacctt cagctcccca tggccaggga ccaccgtttt cacccccggc ccatggtagc
    39721 cgccgacgag tccgtgcacc tcaaacgtcg gcgcggccca aatacggcgc atgacctctg
    39781 ccggatcctg ggtgcgaagc gtcttgaatc cataggcctg cttgaacttg ctcacttgaa
    39841 atccagactg caggaaactc ttgatctctg cctttgtcgg ctcgaccaca tcttcgtaga
    39901 agcccggaat cttcacctta ccggtcttgg catccacgca ggcatggacg atgtccatca
    39961 actcggccag cgggtttcgc gccgcgccgc cggtcacgcc cgaatgggcg tccttggagc
    40021 cggtacgcag gaggaggcgc gcgcccagca gcccccgcag tccatagggc atcgcaggct
    40081 gattcttcga cagccagatc gtatccgaaa tcaccaccga gtccggacgt gggatcgccg
    40141 cacgattctt gatggccgcc gcaaagcttg gactgccgtt ttcctcttcc agttcccaca
    40201 agaatcgcac gttgatgggc accccctgct cgatcgcata acgcgcgccg aacaaggccg
    40261 ccaacgccgg gcctttgtcg tccgtcgccc ctcgtccccg gtatataccg ttgtcgtttt
    40321 gaaacgcaaa gggggcctgt ttccattccg gctcctgcgc cggctgaaca tccatgtgat
    40381 tgtagacggt cacggtcggg tagctcggat cgaccgtcca tccgcctgac acgaccgggt
    40441 agcccggggt ctcgacgatg tgcgtctccg ccccggcggc ccgcagatat tgcgccgcca
    40501 gttccgccat ccggcgcaca tccggcgtat gtcggggatc catgctgata gagggaattt
    40561 ccactgcctg cccgagcatg tcttcgtagc gcgggcgaat gtccttaatg taggtatcta
    40621 actggcgctg caagtcgctc atcgctgtgc ctccctgtga tcgccggcga ttatatcgac
    40681 tcgcaggcgg aaagcctatg agggcgatga tcgagacaaa gaatggtaga atgcgctcga
    40741 tgatccacga tggctcccag atcctgaatc gctctattgc gaaactgctc ttcggcctga
    40801 cggcgttcct ggtcctccat accgacatgg tcgccgcgca agaagccgtc accgtgcagg
    40861 aaatcctgac tgatcccgcg ctgttccact tgcggcagat cacgttgcga gggacagccc
    40921 gcaacgtcca gccgctcgat ccctatgaga tccccgccgg ctcgacctgt tatggcggct
    40981 atctcttcaa cctggaagat gacacggcca cgctcccggt cgcggtcccg ggactctgcg
    41041 gcgtcccgat ggtgaaggac cctgacgtgg aagacggagc gcgcgtggta gtggaggcca
    41101 cggtccaggc cccgagccat ggagggtatg ccttgagctt caagggcgga aagatcgcca
    41161 tggatcagga gggcgtcgta caggccatcg cgaaccggat tatgccgctg gtggaataac
    41221 tcatggcgct tcctctccct gaacaatctc cccgcctcgt gactcccgca cagacacacg
    41281 tcggctggat cggtaccggc ataatgggcg cacccatgtg ccagcatctc caagccaacc
    41341 agtatcgact caccctctac accagaaatc gcagcaaggc atcgctgatt ctggccaagg
    41401 gcgcaacctg ggccgattcg ccgcgcgcag tggctgagca gacagaggtc gtggtgacga
    41461 tggtgggatt cccccgtgac gtacgcgacg tctacttcgg caagcagggt atcctggcgg
    41521 gcacacgtcc gggcatggtg ctgatcgaca tgaccaccac cgaaccgtcg ctcgcgcagg
    41581 agatcgccca ggcggctcaa tcacgcgggg ccttcaccgt ggatgcgccg gtctccggag
    41641 gcgatgtcgg cgcgcgcaac gctaccctct ccatcatggt cggagggacg accaacgccg
    41701 ttcaagccat catgccgctg ctgcaatgcc tcggcaagac aatcctccat cagggcggcc
    41761 ccggcacggg gcagcatgcc aaactctgta accaaatcgt catcgccggc accatgatcg
    41821 gcgtctgcga gagtctcctg tatggctaca aggcggggct gcagctagat cgcatgctgg
    41881 aatcgatccg cggcggcgcg gcagcctgct ggacactgga caatttagcg ccacgcattc
    41941 tggcccgcaa ttttgacccg gggttcttcg tcgaacattt catcaaagac atgggcattg
    42001 cgttggaaga agcgcaacgg cgacagctca ccttgcccgg actcacactc gcgcatcaat
    42061 tatatgaaaa ggtacaagcg ctcggccatg gccgatccgg cacccatgcc ctaatgctgg
    42121 cactggaaga tctgtcgcat atcaatccgg tcaggccttc ggcatcaacg taaccacggg
    42181 agttctatga gaaccgttcc tctgcgcatt cttctccttc ttctcttgtc ggtcacggtc
    42241 tccgcctgca ccggagcccg cggtctcaac cgtcagctcc tgcaggaatc gtttcacgat
    42301 catcccgagg tggtcaccga tcgtgatatc gcatccacca tgaccttgca ggcgaacctg
    42361 ccgacgccct atcgcctggc tgtttttttc aaacaggaag attttccaag ccgcccggcg
    42421 ctgcaacggg tggactgggt cagcacagac gccgaccgtg tgcaacgggc gctcgcgccg
    42481 gtacaggaag aaggcattct tcagcagagt ttcctcctcg ccaactccac ggtccaaggg
    42541 accacactcc gtgacattcg cctggccgcc gcccgctaca acgccgatgc gcttctgatc
    42601 atcgatgggg cctccgccgt ggaacggtac aacaacgggc atgccgcctg gtacgccacc
    42661 ggcatcggcg cctacttcgc ccacggcacc gaaagccacg cgctgttcat gatggaaggg
    42721 accttgtggg atgtccgcac gggctatctg tacggcacgc aaacagcgga aggggaaacg
    42781 accctcgtcg gcccggcgcc atcgcttgag gacaggaccg cagtggccga agccaaggac
    42841 gtggcacttg aacggttcag caaagaactc gccgaaatgc tccggctctt gagggagaag
    42901 cagagagcga tgaagtaaga cttctaccgc tccttattcc gattgtggcc cggatgtttt
    42961 ctgcgcatcg tcgcgaggca gttgaagccg aagcccgccg cggaacttgg tctctcgggc
    43021 gctcaacatg gctgtcgggg aaggccgcag tgagcgaggg acgaggcgta ctctcggtgg
    43081 tacgtcgagg tcctgagcga gacgagaacg acgcccaaag ccatggtcag cacccgatca
    43141 cggagagtcg aaggtccaga gaatgcccag caataccaga tgctgattcg gcgtcaggct
    43201 cagcacacca ggctggatct ccccacgcct gaagaatccc ccgcccgccc cggtcgattc
    43261 gtcccatcga tgttcaagcc gcagcgtcgt gttggtccac ttgtagggga ttcgatattc
    43321 gatcgttgag gtcacggcct tgacgaattg ctccgaaccg gtccaacggc cgttccggtc
    43381 ccaatagaac tccggccgga ccgccagagc ccaaggtccg gtcacatgcc atcgcgcgac
    43441 cacattgcct cccatcacaa atgccctggg atttccggga cgatcggcga tgttttcggt
    43501 cccgatatca taggaagccg cgaatgtcag gttctcccct ttccattcga cgatgtgatt
    43561 cgcgtagaag cgccagaatt gcagtgccgt gttggtctga tccggccccc cgtagaccgt
    43621 ctgggtcagg gtgagttgcg gagtcgcttt gtaggcccac tgccccccat aactcggttg
    43681 gtcgttcgga tgcgacaggt gatagtatcc gttaacgata aaccccgcga ccgtcagctt
    43741 gtcggtgacc ggatatttcg cattgacgcc gaacatcatg tagggcgtgt tgtcggcaat
    43801 ccatgaccgg gtgtagttca cattgtcctt ggcatagagc gattcgtagc cgatgaggct
    43861 attaaaaagc ccggcggtga tcgtgagccc cttcccgacc ggagccagat aggacacatt
    43921 cgcacgatgg atgtgccgaa gcgtatcggc cccgccgact tcacgctcac cctgtaaaaa
    43981 cgcaaagcgt tcggagtcgt agccgccttg cacaccgagt tccatgcccc aacgcgatga
    44041 ttcagtcgcg tctttccgaa cataggccag ggccatattg ggagcaaact cattgtgccg
    44101 cgccgccgtc gcacgattcc gccagaggtg attctctgga aagtttggat tcacgacgta
    44161 gccgacatca aggtaggccc cgtaatgcca gaggtcagtg ccggttgccg gaatcggatc
    44221 gggtgcgatg ctgatttcac cggcatgcgc cggtacgacc aacgtacaga ggagaagtac
    44281 ccaccagagt cggcgcaaaa aaccggcagg agcagtctcc atggctgagc cgacatcgtt
    44341 atgcaacagc aaagcaggtt tcatctgaca ctcatcgctc caccttctca cggcatagac
    44401 attaggtgat gtcgcatcaa aacaggatta aaagcgaaca tggaggcgta aagaatctgt
    44461 aaaagtccgt tggagctctc attctgttcg gagacgatat ccgacaccca attccgtcac
    44521 taaatggcgc ggctgagcgg gatcggcttc gatcttattc ctcaattgac gcatatacac
    44581 acgcaaatag tgtccctcgt ctacatgaag gggaccccac acttctttga gcaactgacg
    44641 atgcgtcacc accttcccgg cataccgcac gagcgtcgtc agcaacttgt attcaatcgg
    44701 cgtgaggtgg atctccgccc ccgagacaaa cacttgtctt cgccctaaat cgacggtcac
    44761 atcgccgaac gtacagctcg cgtccacccc gtctccaacc gggcgagtcg catgccgaag
    44821 agccgcgcgc atgcgggcca gcaattcatc cacgccaaac ggtttcgtga cgtagtcatc
    44881 ggcaccaaga tcgaaggctt gcaccttgac ctgttcctgg tctcgtgcgg aaagcacgat
    44941 gatcggcatg ttcgaccagg cgcggatttt ctcaatcact gcggtgccgt tcatatcggg
    45001 aagaccgaga tcgagcagaa taatgtcggg attgcgagcg gaggcctgcg ccagtccatc
    45061 cgcgccggtg gtcgcttcgt acagccgata gccatgagcc ggcaaagttg agcgaaggaa
    45121 ccgcctgatc tccgcctcat cttcgatgag cagcacggtc acgtcatggc tccgcgtcga
    45181 tgacatgtgc aaacagatcc taggtgactg cgcggtcatg cccgttctgt tccggttcga
    45241 ccgtcggctg attgtgctct gtcggaagcg tgaagcagaa gctcgatccg ccgccggagc
    45301 gggcttccac ccaaatgcgc ccgccatggg attcgataat ccctcggcaa atcgtcagtc
    45361 ccagcccgac gccaccttcc cgatcgggat ggagttgata aaacttttcg aagatcctcc
    45421 gctcttcccc tcgcggaata ccgggccctc gatccgcgac ttccgtcacg atcatccctt
    45481 ccttcaccga ggccgtcaca tcgatggggg aggccggagg agaatatttc gcagcatttt
    45541 ccaataaatt gatcagcact tgttcgatca agacgccatc cacgagcacc agagggagac
    45601 tgtccgggaa cgttgtcgac accggatgtg tagcgatgcg cgatttcact cgagccaacg
    45661 ccgtaccgac cacttcctcc agcgcatgcc attcctttcg cagctgcatg gcacccgcct
    45721 ccaatttggt catatcgaga aggttcttca gcaaccgatc cagacgacca gcctcatcgc
    45781 tgatggaccg gaccagttcg tgttgactct caatcgacag gggccgggtc gtgttcagta
    45841 tcgcgctgct cgctccgaca atcgtggcaa gcggcgtcct gagatcatgg gacactgagc
    45901 tcagcacagc gtttcgcatg cgctccgttt cagtctgcac gtgggcttct tgtgtttctt
    45961 ccgccaggcg cgcacgttcc aacgccaacg ccatctgatt cgcaaaggtc tcgagaagat
    46021 gtagctgctc cggagccagt aattcggcgg gtcgcgacga tcgcaccgcc aggacaccaa
    46081 ccgccccatg agatcctaac aatggcagat agagcgaatc cgatccggaa agggtattcg
    46141 tgccatgacc agcagattct ttatggtcga agacccattg cgagatgccg gcttctttcg
    46201 gatcgaggtc aaagtggagc gcatcgcctc gatggagatg cacccgcccg tctcgatcgg
    46261 gcagaaacac cgccacctgg caatggaaca cgtcgtggat atgacgggac gcgacagaag
    46321 ccagcttgtt cacttcacgc tgagtgatca attctctact catggcgtag agcgcggcag
    46381 ttcgttgttc acgaatcaag gccgactcgg cctgccggct cttgtgagac gcaagcccgc
    46441 tgatcagcag cgcgaccacg agcatgaccg caaatgtcag cagatattgc acatcggaaa
    46501 cggcgaatga atagtaaggg ggaatgaaaa agaaatcgaa ggccgcgacg ctcatcaatg
    46561 acgccagcgc cgaaggacct cgtccccatc tggtggcaat gaccaccacc ccgaacagat
    46621 acaccataat cagattggcc tgcccgaagt atggagccat caccccactc agcgccgtgc
    46681 aggccatcac caacgccgag gcgtagccgt agttccggcg ctcactggtc cgttgaagta
    46741 atccggtcac gagaggttga ccggtccccg cttcaccggt catgacgtag atatcgatat
    46801 ctccgctccc gcgaacgagg tcgtccacca ccgaaccgaa cagccattcc ttccagcgcg
    46861 agcgcaatgg ttttccgaca atgattttag tgacattgcg gctccgggca tacgccaaca
    46921 gctgcgcact cacgtcatcc ccggtcaacg tcactgtctc tgcgcccata cgctcggcca
    46981 accgcagggt gtcgacacct tggctccgct cctgctcagt caaccgcaga tgtatggacg
    47041 tttccacata gaccgcgatc cacctggcat ggaggcccgt agccatcttt ctggccgcgc
    47101 gaaccaacat cggcccgcgg gattttagat tcacgcagac caggatggtt tccgctgccg
    47161 gccaggtgcc gacgaccgca tggtcccggc gatacacttc catttgttga tcgactcgct
    47221 cagccgtccg acgcagagcc atctcacgca acgcgatcaa gttgcctttc cgaaagaagt
    47281 gtttgatggc atgctgcgcc tggtccggca gatagacctt gccgtccttg agccgttgca
    47341 ggagatcgtc cgggggaaga tccaccaatt cgacgtcgtc agcttgttcg agaatggaat
    47401 ccggcacggt ttcgctcacg cgaataccgg taatttgggc cacggcgtca ttgaggcctt
    47461 cgatatgctg cacgttgacg gtagtataga cgtggatacc ggcctgcagg agttccctca
    47521 catcctgcca ccgcttggga tgcctggacc caggggcatt cgtatgcgcc agttcatcca
    47581 ttagcaaaag ctgcggccgg cgcgacaggg ctgcgtccag atcgaaatca cggagcctgg
    47641 agcctccata taccacttcc cgggatggaa gaaccggcaa cccctgaacc agggcttccg
    47701 tttcggcccg gccgtgagtc tcaacccacc cgatcatcac atcgatgccg tcgcgttgct
    47761 gctcatgtgc cgcttgcaac atggcatagg tcttcccaac ccctgcagtc gcgccgaaaa
    47821 acaccttcaa cttgccgcgc acctgttcgg cctcttcagc ctgcacgcgc ctcagcaagg
    47881 catcggggtc cggacgctgg ttctccatgg cactcctgac aaaggaatct gatgatggat
    47941 gcgagatgac acaagattgg cagagcgaat tctaaaagac agacgtaaaa gcgtcaacga
    48001 ggagggtcgg tcgcatccaa tgcaaggttc aacgccagca cattgacccg gcgttcacca
    48061 agcatcccca gctgacgtcc ttcggtatgc tgactcacca acgtccggac gaatgcttcc
    48121 tccctccccc gtgcacgagc aacccggcgt acttggtatt cggccgaagc ggggctgata
    48181 tgtgggtcga gaccgctacc tgaggcggtc acgagatcga ccggaaccgg tgtgtcattg
    48241 ccgggatccg cggctttcaa cgcagccacc ctcattttga ccgtctctat cagcgcggga
    48301 ttggtagggc ccagattcga accggatgag gccgccgcat tgtaggggaa gggagcggtc
    48361 gcggaagggc gtccccagaa atacttggga tcgtcgaagg gttggccgat cagcgtagaa
    48421 ccgatcggct tgccgtcttt agagatcaga ctcccattgg cctgatgggg gaacagcagc
    48481 tgcgcgattg ctgtcacggc cagcggatag gccacgccgg tcaggatcgt cagaatgaaa
    48541 aacatcatca gcgcggatcg aagttgaacg accattgtga tctcctcaga ccaatcccag
    48601 ggcgaccagg accaggtcta tcagctttat tccaatgaac ggggcaacga tccctcccag
    48661 tccgtacacc agcacattgc ggcgcagcag caaggaggct tcgatcggcc ggaacttgac
    48721 tccccgaagc gcgagcggaa tcagcgcgat gatgaccagg gcgttgaaaa tcacggccga
    48781 cagtacggcg ctttccggcg tcgccagtcc catgacattc aacgcgccca gggccggata
    48841 ggtgccggca aacgccgccg gaatgatcgc aaaatacttc gcgatatcgt tagcgatact
    48901 gaaggtcgtg agcgcgccgc gcgtcatgag tagctgtttg ccgatttcga cgatctcgat
    48961 caatttggtg ggattggagt ccagatcgac gagattgccc gcttctttgg cggcctgggt
    49021 gccggtattc atggccacgg ccacatccgc ctgcgccaac gccggcgcgt cattggtgcc
    49081 gtcgcccgtc atggccacga gtcggcctcc tgcctgaaga tcacggatga gtttcaattt
    49141 cgcttcggga gtggcctgtg cgagaaaatc gtccacgccg gcctctgcgg cgatcgcggc
    49201 agccgttttt tggttgtcgc ccgtaatcat catcgttttg atgcccatgc ggcgcaattc
    49261 ggcaaaccgt tccttgatgc cccccttgac cacgtctttc aaatggatca ccccaagtac
    49321 cgacttgcct tcggccacga ccagaggagt gccaccttgc gtggcaatcg tctcgaccgc
    49381 atttcgcacg gccggcggaa acgccccccc gagttgggtc acgtagtctt caatggcttc
    49441 cgccgcaccc ttcctgatct gtcgaccgtc aaagtcgacg ccgctcattc gcgtgtgcgc
    49501 cgtgaacggc aggaaggtgg cccccatcgc atggatgtcg cgccctctga gtccatagcg
    49561 ctccttcgcc agaatcacga tgctacggcc ctcaggcgtt tcatccgcaa gggatgccaa
    49621 ttgggccgca tcggcgacag atctttcctg cactccttcg gctgaaataa atgcggtggc
    49681 ttgccggttc cccagcgtaa tcgtccccgt tttgtcgagg agcagcacat ccacatcacc
    49741 ggccgcttcg acggctttcc ccgacatggc gatcacgttc gcctggatca tgcggtccat
    49801 acccgcgatg ccgatcgcgg ataagagcgc gccgatcgtc gtcggtatga ggcagaccag
    49861 cagggccacc aggaccgtca ccgtgatggg caccccctct cccatggcat gcgcggagta
    49921 gagagaaaac ggcagcacgg tcacggtcgc cagcaagaag acgacggtga gagctgacaa
    49981 cagaatagtg agtgcgattt cattcggtgt cttctggcgc ctggcgccct caaccatggc
    50041 gatcattcgg tcgaggaagg tttcaccggc gctggcggtg atccgcacca cgagccaatc
    50101 ggacagcacc cgcgtgccgc cggtgacggc gctccggtca ccggactcac gaatcaccgg
    50161 cgcgctctcg cctgtaatcg cactttcgtt gacggatgca atcccctcga tcacatctcc
    50221 atcgctggga atgtcatcgc ccgcctccac cagcacgatg tcgccttttt tgagaaaact
    50281 ggccggaacg aggcaataga cctcgccggg tttgccgatg gcggcgggca ccgccagata
    50341 gtcttctccg gaagtcggtt ttccaaatcg cttggcagtc aattctgtcc gagccctccg
    50401 gagagaatcg gcttgagcct tgcctctccc ttcggcgatc gcttcggaaa agttggcgaa
    50461 gagcacggtg aaccagagcc acagcgagat ggcgaggatg aaccaggtcg gcgcctcccc
    50521 tgtcccgaac aatgcctgga ggaacaggag cgacgtgaag aggctcccca cgaagacgac
    50581 gaacatgacc ggattgcgga
//
LOCUS       DAOQRM010000003        49363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_329_length_49363_cov_15.123753, whole genome shotgun sequence.
ACCESSION   DAOQRM010000003 DAOQRM010000000
VERSION     DAOQRM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 49363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49363
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_329_length_49363_cov_15.123753"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKK81_00495"
     CDS             complement(<1..384)
                     /locus_tag="PKK81_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNV31372.1"
                     /translation="MCTRVLTILTLWMALFFGIEPVSAQTPLPKPDHIVIVIEENHSF
                     AQIIDSPLAPYLNSLARKGALLTNSYGITHPSQPNYIALFAGSIEGVNGNTCPLALTA
                     PNLHSTLTQAGQTFAGYAEDLPAVGA"
     gene            complement(401..2131)
                     /locus_tag="PKK81_00500"
     CDS             complement(401..2131)
                     /locus_tag="PKK81_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV31373.1"
                     /translation="MFSFNRFYPFLKPYVPRMMAAAVMVMAVAAINLALLRLGGTLWD
                     VITVQHDAARMTEMILLLLGLVLIQGLCSMGHSYLTAWVSQRVMADFRIHLFAHLETL
                     SVNFFSKRRTGELMSRLMNDVTVIQNVVTDTPIDAAKQIVTFIGGAGFLFAMNWQLCL
                     LILVLLPMLVVVAKLFGRRLRALSTKIQDQTASVSTLVEEVIAGIRVVKSFVQTKREE
                     ERFVAQVQSAMELSLRRATIMAWFVPTIIFVTFAAAALVLWYGGRQVIDGTVSPGDLF
                     AFVLFAGILIGPFGSAARVFAQIKEAQGAMQRVFEILDTHAEIADAPGAVDLPPIRGH
                     VRAEHISFAYDPRQPILSDLSFEAKPGELIAIVGPTGSGKTTIMNLLHRFYDPTAGRL
                     TVDGHDVKDVRLDSLYRQIALVPQDTILFGGPIRDNIRYGREDASEEEMIAASKAAHA
                     HEFIVGFPDGYQTIVGEKGINLSGGQRQRVAIARAILKNPRILLLDEATSALDTESER
                     LVQEALERLMVNRTTFVVAHRLSTIQRADRILVLNKGKIVEEGTHAALLEQRGLYHYL
                     YTLRLSELPV"
     gene            complement(2235..2453)
                     /locus_tag="PKK81_00505"
     CDS             complement(2235..2453)
                     /locus_tag="PKK81_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31374.1"
                     /translation="MPEGKKVRIRVRTVNCVYVGDFLIPPMRNRVSDAINEEQRLFIS
                     LTDVLINDKDRSDFVAINKNLIESIAQL"
     gene            complement(2485..2580)
                     /locus_tag="PKK81_00510"
     CDS             complement(2485..2580)
                     /locus_tag="PKK81_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HNV31375.1"
                     /translation="MASVLKKRRKKMRKHKYKKLRRRQKFLRRKS"
     gene            2756..3271
                     /locus_tag="PKK81_00515"
     CDS             2756..3271
                     /locus_tag="PKK81_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNV31376.1"
                     /translation="MIESCPPISLRLGRVFLALLLIMTVGCAGKTLQYKDDHDRILRI
                     DQAVELLRKSYVERDRSALEALLLPSGNLDQVQREIHADFDTFRDIQLEFSIERILIE
                     GDNIDVFVHWQGQWKRDPAEVGTRQRGHGRLAWVGTQSILLREAQGDLPFGMGARASV
                     GEDPARGQAKP"
     gene            3274..4950
                     /gene="nadB"
                     /locus_tag="PKK81_00520"
     CDS             3274..4950
                     /gene="nadB"
                     /locus_tag="PKK81_00520"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNV31377.1"
                     /translation="MSARLSSSKIETDFLVVGSGVAGLRAAIELSRAGRVLMLTKGHP
                     LESNSMYAQGGVAVALSEEDDVGSHLTDTLKAGHGLCRREAVRVLVEEGPERIQELIA
                     WGAKFDKIGKRFAFTREAAHSRSRILRARGDATGNEMVRALMSYAARQPRIQRLDRRF
                     TVDLAVVDGRCCGAIVLNEMTGERTVLPASAVVLSTGGAGQVYARTTNPGNATGDGMA
                     MALRAGALLMDMEFVQFHPTSLYLPSSPPFLLSEAIRGEGGQLRNIKGELFMHRYHPD
                     GALAPRDVVSRAIWSEMAATRARHVYLDVTHLGAAFVKRRFPTIYATCLRYDIDMTEE
                     WIPVSPSAHYMMGGVWTDVQGATTLPGLCAAGEVACSGVHGANRLASNSLLEGLVFGM
                     RAAQSAVAHAGRFPGPVRMPHLEELEQGRPTDFDDPEKLRSSLRRLMWGKVGLVRSGD
                     SLISASAQLVRWMRLLTKPFASRAALEVKNMVQVARCITDAALWRENSVGAHYRSDFP
                     EPNRPGWRQHSQLLFTDGQTSGSAAKERALVLSPPAPVRGKPGKKKAGRS"
     gene            complement(4841..5521)
                     /locus_tag="PKK81_00525"
     CDS             complement(4841..5521)
                     /locus_tag="PKK81_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNV31378.1"
                     /translation="MAHFSRTAALFSVAAALLLNALPSQADVYQYIDANGTISLTNVP
                     NDPRYRRVIAELPRSRAVISDGELEPVIARHSRAHRLHPALIRAVIKTESDFIPQAVS
                     RAGAVGLMQLMPQTAVRLDVRDSYNPDENIGGGTKYLRQLLDRFNGNLPLALAAYNAG
                     EHTVERYQGLPPIPETRQYVQKVLRYYRTFLTNDRLSSSRAYRAQALGAKARALAPSL
                     PTRSSDRP"
     gene            complement(5673..6554)
                     /locus_tag="PKK81_00530"
     CDS             complement(5673..6554)
                     /locus_tag="PKK81_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNV31379.1"
                     /translation="MWVFLNDRFVRKEEALVSVFDHGFLYGDGVYETLRSYGTRIFMR
                     DQHLARLRRSAEAIGLDIPIPESHWPELLHEAMRRNGVGNEQTDAYLRITVSRGAGEI
                     GLDPGLCPRATVVIMAKPLHPSPPVLSRDGVSLTVANTRRNLPEALSPQIKSTNFLNN
                     ILAKRESIAAGTFDSLFLNWKDELTECTVSNLFFISGKTLHTPALDCGILDGITRTIV
                     MTLAQEEGVPVREGHYHLADLQRAEECFLTNTSMEIIPVNRVDSFAIGDGRPGSLTRR
                     LQTRFADSRTRFLEPPS"
     gene            complement(6554..8038)
                     /locus_tag="PKK81_00535"
     CDS             complement(6554..8038)
                     /locus_tag="PKK81_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNV31380.1"
                     /translation="MSFSSHQAFLSGPPQPLVVVRPQPDVDAFELYCRLAPAERPSFL
                     LESANGTDTTARYSFFGRDPYLTLTSRAQEYQIETGGQIRRYQGSGIQALQQALAEST
                     ITKPEGLPPFYGGAVGYLSYDLVRSFELLPTLAADDLHLPELHMAFFDVVAAIDHHTR
                     QLYLMYCPPLSRFQAEPREKLYREGCDRLAELEARLTSPVTPLTSSPWPTQTTFVPSQ
                     SREAYKTRVRRCQDYIAAGDIYQANLSHRFTLDLHAAASPTGFEGYSTELYRRLRRIN
                     PAPFAGLVRFADLSLVSSSPERLVRLTGSQASTRPIAGTRPRGTGLQQDQLLRAELLA
                     NPKERAEHVMLVDLERNDLGKVCRYGSVRVDEFMTIEQYSHVSHLVSDVVGTLQPGVS
                     PLDLVKAVFPGGTITGVPKLRCMEIIEELEPVRRGLYTGALGYFSWNGDLDLNILIRT
                     LVLTKNRGYLQVGAGIVADSDPDREYDETLAKAGAFFKILEAGR"
     gene            8214..8432
                     /locus_tag="PKK81_00540"
     CDS             8214..8432
                     /locus_tag="PKK81_00540"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNV31381.1"
                     /translation="MPPSQDPLYAGLGQAVRIGTELLAALIVGGGLGWVVDTYLLDSN
                     PWGLVVGLGLGAAAGVRSAYRSAQRWQS"
     gene            8463..9212
                     /locus_tag="PKK81_00545"
     CDS             8463..9212
                     /locus_tag="PKK81_00545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNV31382.1"
                     /translation="MEESPLHAFELHDLIPLVPAGVDISINKAVILMWVIVGLVAFLM
                     ISAAAARKLVPGKLQNMAELIVEFIRGIILDTMGEPGMKYFPLIATLFLFILFANLIG
                     LIPGSYTVTSQIIVTAVFAVGVYGLSIVLGFSLHGMKFLGILVPPGTPGWLLPLMIPI
                     ELISQLARPISLAVRLFANMTAGHVILGVLFGLAISGGLLIGWLPFAFTIAMNGLEVG
                     IAFIQAYIFTVLSCVYLGDAVTLHGHSEHAH"
     gene            9249..9479
                     /gene="atpE"
                     /locus_tag="PKK81_00550"
     CDS             9249..9479
                     /gene="atpE"
                     /locus_tag="PKK81_00550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNV31383.1"
                     /translation="MDAAAAALVGMGLAAAGFAGAGVGIGYIFGKMIEAVARQPEAEG
                     RVGKYMWIGFALVEAIALYGLVIAFIIMGLRK"
     gene            9554..10066
                     /gene="atpF"
                     /locus_tag="PKK81_00555"
     CDS             9554..10066
                     /gene="atpF"
                     /locus_tag="PKK81_00555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNV31384.1"
                     /translation="MPQFESHFFSSLIFWEILSFGILFFLLYKYAFPSLLGMLEEREK
                     KIKDSLDQAERHRSEAERRLKEYEAKLATAAKDAEALLAQAKERAQRLMEENEQRMTA
                     DAERIKGDATREIETERRKAIQDIRSQTTDLAMMVAEKVVGRALTEGDHRRLADEALD
                     ALAKSYQSRN"
     gene            complement(10073..10894)
                     /gene="larE"
                     /locus_tag="PKK81_00560"
     CDS             complement(10073..10894)
                     /gene="larE"
                     /locus_tag="PKK81_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNV31385.1"
                     /translation="MVSTTPLIDKVSQARQILREMGSVIVAFSGGIDSSLVLKLAHDE
                     LGAQAVGITAVSPTLPASELDATQKIAAEIGARHRIVETDQLQIPEFVLNDATRCYHC
                     KTDLYSLLETLRSEYADGYIVDGTNVDDLGDDRPGLKAARERGVRSPLLEAGFSKADI
                     RDVARELHLSNWDKPAAACLSSRIPRGITITRSTLSRVERAEDALAQEGFRQYRVRDH
                     GEIARIELAVEELLGLLQPGRRERLAETLKKLGYRFVTVDLEGYRQGGVSLTPPA"
     gene            complement(10938..12320)
                     /locus_tag="PKK81_00565"
     CDS             complement(10938..12320)
                     /locus_tag="PKK81_00565"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV31386.1"
                     /translation="MTQQPATLLPRQHRSRRPVGRLLAGLLCMALSLPFALAQAADIV
                     PTRSVTANGMTVLFLEQHFLPTVEIHALVKVGSAQDPPDKAGLANLTASLLDEGTLTR
                     TSRQIAEQIDFVGGSLEAHAAEDFTTASTRVLKKDADLGFALLADMLQHPAFHKQEFE
                     RVRTQILGEIVSDDDDPGNVAMKAFHQLIFHGHPYSWPAHGTEETLNKITVADIQQFH
                     AREYLPNQTILVIVGDLTQDQAAALVQTHFGSWKKGPPSPYQIKKPASIERKMVQLIE
                     KDLTQSTIVLGHTGISRTNPDYYAVTVMNYILGAGGFSSRLMDSIRDKQGLAYGIMSQ
                     FDTRLMPGAFFISLQTRTDVTNQAIAGVLTEIKGMRDAPVTDQELNEAKSFIVGSFPL
                     RVDSSAKLANVLAQVELYNLGLDYFTQYPKAIEKVTKDDVLRVAKQYLDPQHYALVVV
                     GSIAKAKVKQ"
     gene            complement(12333..13724)
                     /locus_tag="PKK81_00570"
     CDS             complement(12333..13724)
                     /locus_tag="PKK81_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV31387.1"
                     /translation="MPNQSTTSNSAPVRFSILACSLLSFILFASQTSPAHAVEPKEYT
                     LSNDMKVILVEVPKAPVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTAKYPKGTF
                     SRLVRKNGGMDNAFTSQDFTAYFENLAADRVTLALELEADRMQGLILDANEFKTEREV
                     VKEERRLRNEDDPQGALVEALFAQAFMSHPYHWPVIGWFSDLDAMNLDDLQRHYDTYY
                     SPNNATLIVVGDIKADTLLPTIAKLFEPIPKGPSPKPLAVTEAPQHGERRFLLKREAQ
                     VPFVMMGYRVPNYSSDDSYALNVLESILSHGKSARLYQSLVYEQKTALAVGADYGLMQ
                     ADPGLFYFYAVVKPGEKVEAVEDAVLKEIQRIQTEPPSELELQRAKNQIEAAHIFEQD
                     SNFRQAMLLGEAETIGAGWRKVSQFVERTRAVTAQDVQRAASHYLAADMRTTGTLIPQ
                     PPQTQAASPAQPH"
     gene            complement(13739..14827)
                     /gene="rlmN"
                     /locus_tag="PKK81_00575"
     CDS             complement(13739..14827)
                     /gene="rlmN"
                     /locus_tag="PKK81_00575"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNV31388.1"
                     /translation="MLQRTDNRTNLLAVTESGMAAFVASLGWPAYRASQILRWLYQER
                     ARTFAEMSNLSQKDREYLTGSCSIERTSAVQIFSSQDGTKKFVLTLADGNQVECVLIP
                     DEDRLTLCLSTQVGCTLDCGFCLTGTLGLQRNLRAHEILDQVLLAQDHLQEGHRLTNL
                     VFMGMGEPLANLDAVADAVTRLTNNTWGLGFSGRRITISTAGLASRIKDVAPLKVNLA
                     ISLNATTDALRQQLMPAANRLHSLDALLEACRAYPLADRDRLTFEYVLLADVNDRAED
                     AARLVKLLRGLRCKVNLIAFNPFPGNPYRRPSDAAIETFQATLRRGHVDVYLRRSRGR
                     DVLGACGQLGRLDTSEAHVALTQIQTRC"
     gene            15140..17176
                     /locus_tag="PKK81_00580"
     CDS             15140..17176
                     /locus_tag="PKK81_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNV31389.1"
                     /translation="MMLKIDQLRSVMELYPSTIWAKRAGVVLGVLRIEREPVEAAKLL
                     RVVQPDMPVLDDYLRLWIGESLLKQNEPIQAAELLETIPKIVPDSSLIAKAAYRTGEA
                     WYSANVCFRAVDWLERAVALADKDPAAPLALWHLAECYIRENRLPEARTALKQLWLRY
                     PHSPEAREAKARLDTALGGESWTPTAEDHSIRAQAFLGLAMQAEAVEELRRFLVMAPG
                     HPRRFDARLKLGVAYVRLKQYDQARETFRGLVADRVQESSEATVWLARVYLRQNQGDK
                     LIALARSVAQGSLGGDQRAMVHLFAGVWLEDQGQFDEAIGMFRQVAKLGDSASQRAEG
                     LWRAGWAQYRTARYRDAAETFRSVVELHVNGFEPQAMYWAARADEREKNTTVADQYTR
                     LCQRHAYSYYCQLAARRVSLSPVTPVATDAERPAGDEASRLPENRRPEIERHVVYQRG
                     IELKILGFAQDAARELGSLTEQYSRDPEVLLAFSTLLSEVGAYHPALRVAKVHFKEKL
                     ERSGLPTAPALWTVAYPTGLLPTITAQGVTAVDPYLAAAIIREESQYDEKAVSMVGAV
                     GLMQLMPVTANAVAQRYGFPAVGREELFDQETNIRLGVRYLGQLLEQYSGNLAHAVAA
                     YNAGPIAVNNWIAVHRGREQDEFVELIPYQETRLYVKRVLRSYGEYRRLHNGTS"
     gene            17271..17525
                     /locus_tag="PKK81_00585"
     CDS             17271..17525
                     /locus_tag="PKK81_00585"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HNV31390.1"
                     /translation="MTLDHLDALEIRIRDLVKLVQDLKRKNASLEDDLRLARERVAVR
                     DDENRRWEQERIDIRSRIEKVLGEIDLLECLDEPKEVAVD"
     gene            17518..17814
                     /locus_tag="PKK81_00590"
     CDS             17518..17814
                     /locus_tag="PKK81_00590"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNV31391.1"
                     /translation="MTKTIDVEIYGQRYSINGEADESYVKQLADMVDKQMKQVAAGMR
                     SATPAKLAVLAAFNLAHELMESERKSRQGEADADRRVASLMESIDQQMPSILSR"
     gene            18374..19951
                     /gene="rny"
                     /locus_tag="PKK81_00595"
     CDS             18374..19951
                     /gene="rny"
                     /locus_tag="PKK81_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNV31392.1"
                     /translation="MVPISLSVVAYIFVGLVGAVLGAGLYEVFRRRSALARRAEAEDQ
                     SAQIVQSAQREAENLVKEAKLEAKDLVFQARIELEKEQKAKLAEVSNSERRVSQREEG
                     LDRKLGLLEKRDQEALKREQDLLKREETLVQKDAACAQALKQHREALERVAGLTAEEA
                     KRQLIQDLDSQARLEAAGLAKRLLEEAKENADREAREIIASSIQRVTRDYVNEATISV
                     VPIANDAMKGRIIGREGRNIRAIEAATGIDLIIDETPEAVIISGFDPLRREIAKVSLE
                     RLMHDGRIHPTRIEEIVEKVKVDIEKLMIEEAEKVIFEVGLSDFHPELVKVLGRLKYR
                     TSYGQNNLYHAREAAYICGIMASELKLDVKLAKRGALLHDIGKAVSHEEEGPHAMLGA
                     EIAKKYGENAKVVNAIAAHHEQVEPICPETVLVAAAEALSAARPGARREALESYVKRL
                     EKLESLATVHKGVQKAYAIQAGREIRVIVKQEDLTDPECFQLSRDLAKKIEQELTYPG
                     QIKVTVIRESRFVDFAK"
     gene            19962..20741
                     /locus_tag="PKK81_00600"
     CDS             19962..20741
                     /locus_tag="PKK81_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNV31393.1"
                     /translation="MKVLMIGDIMGEPGRRSVARLLPKLIANHSIDVVVGNGENVAGG
                     FGITPDLVDDLFDLGVSVITTGNHAWDKKEILDVFPREPRLLRPANYPTGVPGRGSYV
                     FTTPGGESLGVLHLMGRAFMPTIDCPFQVAKREIERLKTQVSAIVVDMHAEATSEKMA
                     MGHYLDGLVTVVAGTHTHVQTADEQILPKGTAYITDIGMTGPLHSVIGIKKELAIEKF
                     LTGMPRRFEVASGPVVFCAVLMELDATLGKALSIERIRVVD"
     gene            20779..22137
                     /gene="xseA"
                     /locus_tag="PKK81_00605"
     CDS             20779..22137
                     /gene="xseA"
                     /locus_tag="PKK81_00605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNV31394.1"
                     /translation="MTGQTLPLPLLLSVSDVTRLIRDSLEAQFRDIWIEGEITNLRAP
                     SSGHLYFTLKDEQSQLRGVLFRSGASRLRFTLQEGLVIVARGRISVYEPRGEYQLIVD
                     SLEPKGVGAFQLAFEQLKERLAREGLFDEARKRPLPPFPRTVGVVTSRTGAAVRDIVA
                     VLRRRCPVANILIASVPVQGEGAAEQIAEAITTLSGMPQVEVMIVGRGGGASEDLWAF
                     NEEVVVRAIVQSRVPVVSAVGHEIDVTLADFAADYRAPTPSAAAEAVVPVLDEIVERL
                     GEMSDRLYRVVRTLLEMQRHRFERSVGVMRDMRFRVQAHAQHLDELRDGLTRTLTERL
                     TLLHRGMVVRQHALLAQGPHNRIQTALVVIPQLYKRLEQEARRGLLSRRQAVASYMMA
                     LDALSPLAILSRGYSVIQAIPSGRIVRLASDVAVGDVVQARLAEGRLLCLVSEVLPPS
                     MP"
     gene            22177..22443
                     /gene="xseB"
                     /locus_tag="PKK81_00610"
     CDS             22177..22443
                     /gene="xseB"
                     /locus_tag="PKK81_00610"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNV31395.1"
                     /translation="MSKGVVVAAVKFEYAMARLETIVAELEKGDLPLDDSLKIFEEGI
                     RLSKTCLKMLEDAERKVEILVQEKDGKKRIQAFSPGEDEPERPQ"
     gene            22511..23266
                     /locus_tag="PKK81_00615"
     CDS             22511..23266
                     /locus_tag="PKK81_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNV31396.1"
                     /translation="MSQKLRPERERLDRVLVSRGLVASREDAARLILAGLVRVDGVVV
                     DKAAKPTLSDAKVELTGPGSPYVGRGGEKLAGALDQFQVDPKGMMCFDVGCSTGGFTD
                     CLLQRGAARVYAVDVGYGQFEWRLRQDPRVVLMERTNIRYLEPSAIRDPIDLIVIDVS
                     FISLTLVLPCVVPYLAASGFVITLIKPQFEVGKGLVGRGGIVRDDGLREGAADKVVAC
                     AQQLGLELADRMESPIEGRKGNREILAWFRRRA"
     gene            23438..24361
                     /locus_tag="PKK81_00620"
     CDS             23438..24361
                     /locus_tag="PKK81_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV31397.1"
                     /translation="MKVLVTGGAGFIGSHVVDRLLQEGHDVVVVDNLVTGKRKNVPKA
                     AQFYKLDIENPKLERIFRNERPSIVFHLAAQMNVRRSVEDPMFDAQVNVLGTLNVLEQ
                     ASKHGARKVIFSSSGGAIYGEQLAFPAPETHITQPLSPYGISKLCGEHYLSYYHRLSG
                     IQVVSLRYANVYGPRQDPEGEAGVVAIFIQKMLRGEQAVVNGNGRQTRDFVFVEDVVE
                     SNLMAMGPEVEGVYNVGTGIETSVNDLFKIVVDLTKVEFKEVHGPAKRGEQARSVIDS
                     TKLHRDLGWEPKVDLREGLRRTVEYFRDGLG"
     gene            complement(24371..24691)
                     /locus_tag="PKK81_00625"
     CDS             complement(24371..24691)
                     /locus_tag="PKK81_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV31398.1"
                     /translation="MSFTITPKDLKSRLDKGDKLVLVDVREPWEYAIAKLEGSILVPL
                     ATLQQSLGKLDRDAEIVAVCHHGMRSADATGFLLQQGFGKVKNLIGGIDAWSTQVDPS
                     VPRY"
     gene            25038..25367
                     /gene="cutA"
                     /locus_tag="PKK81_00630"
     CDS             25038..25367
                     /gene="cutA"
                     /locus_tag="PKK81_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNV31399.1"
                     /translation="MGANGSEVVVFVTAATAEEAERLGRIIVESRLAACANVLNGIRS
                     IFRWENKINVENECLMLIKTTLEQYPELEAVIRRHHSYTIPEIIALPVIAGSAPYLEW
                     VREETRK"
     gene            25485..26504
                     /locus_tag="PKK81_00635"
     CDS             25485..26504
                     /locus_tag="PKK81_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNV31400.1"
                     /translation="MSQQAAETSDAYTVVVFRGSSSKPLRFSFPRKFVRKLLILAAIL
                     VVADLLVISHYVIRTGEVWQLSAFRAEAMGAREQTAAFSAAIDDLKKRLSTMGEVNQR
                     LRVMLGIDASKPAGDLANGRGGEDGPLPDGKTSVQGNGSATSGLEPRQQVSEARDANQ
                     SEVDFATESIEEVTQQVRESLEALVREAKQQEEALESLTQVAEQRSTQWASTPSIWPV
                     RGWVTSAFGPRVSPFTEKPAWHDGLDIGAQANSPVQAPALGRVVTVAFDSKMGNMVKL
                     DHGYGIETVYGHLAKSLVKEGQRVKRGDVVALVGSTGLSTGPHLHYMVKKNGQALDPT
                     KFILD"
     gene            26702..28384
                     /locus_tag="PKK81_00640"
     CDS             26702..28384
                     /locus_tag="PKK81_00640"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNV31401.1"
                     /translation="MTDLEIARSVPHKHIFEVAQSLGIHSDDLIPFGRYKAKIAPTLG
                     ERIQNRPLGRYILVTAINPTPLGEGKTTTSIGLSMGLCRLGHRAAVTLRQPSLGPVFG
                     IKGGGTGGGRAQVHPMEDINLHFTGDAHAVSASHNLLSAFVDNHLFHGNTLKVDPKGI
                     RWPRTLGVSDRALRDILLGEETGRRPGKFVITEASEVMAVLALATDHADLRQRLGRIL
                     IGLTEGGQMVRAEEFGCAGSMAVLLKDALLPNLVQTLEGTPAFVHAGPFGNIAHGNCS
                     IVSDRLALRCADYVVTEAGFGSDLGAEKFFNIKCRTSGLRPDVGVVVATLRALKLHGG
                     GGIVKSGAPLPPGVTGPNQAALEQGFANLEQHIANVRAHGVPVVVAVNAFKDDSTDEL
                     NWVCERALEGGASAAAVSTHWSDGGRGAEELARAVVKVAAQGTQFSPLYEVTSPIKKK
                     IETIATTIYGAAGVSFSPQAERDVELATRLGFDRLPICMAKTPLSLSHDPAVKGRPSG
                     FTVPIQELRILAGAGFLTAVCSGIQLMPGLPKKPAGERIDVDPQSGQIVGLQ"
     gene            complement(28392..29252)
                     /gene="hflC"
                     /locus_tag="PKK81_00645"
     CDS             complement(28392..29252)
                     /gene="hflC"
                     /locus_tag="PKK81_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNV31402.1"
                     /translation="MSKQGFILAFVGIVLGLLVLGASPFYIVDVTQNAIVVQLGKPVR
                     NVTEGGLYLKMPFIEEVTYFDKRLLDYDSNAQDVITQDKKTLLLDNFAKWRITDPLKV
                     YQAFQSQRGALQRLHDIIYSELRVELGRHDLAEIVSSARAQLMAVVTQRANEKASAYG
                     IEIQDVRIKRADLPEQNEKAVFSRMQAERERQAKQYRAEGAEEAQKIKSEAEKDREII
                     LAEAYRESEELRGGGDAKAFRIYADAYRQDPHFFEFTRTMEAYRKTLKDKTTILVSPE
                     SEFFRYLKQR"
     gene            complement(29249..30286)
                     /gene="hflK"
                     /locus_tag="PKK81_00650"
     CDS             complement(29249..30286)
                     /gene="hflK"
                     /locus_tag="PKK81_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNV31403.1"
                     /translation="MVWDPKDPWSKKGDDLDQAFKQAQGQLRNLLPTGGFRNLLLVAF
                     TVFLIWQSAFIVAPDEEGVVKRFGIPVRVVDPGPHMKIPIIESVLQPKVAKLHRVEIG
                     FRKDRQGRQQMVPQEALMLTGDMNILAIEFIVQYKIKSSREYLFNVADIDETIGKAAE
                     ASMREVIGKSKIDEALTTGKAQIQNDTQELLQHILDDYRTGVQVAAVQLQDVDPPEAV
                     AAAFKDVTNAKEDREKLINQAQGYRNDITPKAKGEAAQLVNQAKGYAQARLNRSQGES
                     NRFLATLKEYNQAKDIISKRIYIETLEDVLPHIDKFVLDGKGADRALPYLPLDRFSKP
                     APSSSTQERTP"
     gene            30428..30733
                     /locus_tag="PKK81_00655"
     CDS             30428..30733
                     /locus_tag="PKK81_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNV31404.1"
                     /translation="MRNPARAAELFHALADPTRLEILDELKEGECCVCELTDRLQAGQ
                     SRLSFHLKVLKDAGMILDRREGRWMYYSVNADALAELDDLVDSLKQAKRARRATGCC"
     gene            30790..31614
                     /locus_tag="PKK81_00660"
     CDS             30790..31614
                     /locus_tag="PKK81_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNV31405.1"
                     /translation="MDAQAIRELVKEKYGEAAARAKSGGSSCCGASPALMNVDPITSN
                     LYSDQERQGLPADAVAASLGCGNPTALAELHAGETVLDLGSGGGIDVLLSAKRVGPAG
                     KAYGLDMTEQMLALARENQRAAGVENVEFLKGEIEHIPLPDRSVDVIISNCVVNLSGE
                     KERVLAEAFRVLRPGGRLALSDIVVRGAIPSEIRRNLELWAGCVAGALEETEYRDMLA
                     QAGFVEIGIEPTRIYQADDVKELLVGTDLSSDLLVAQVEGKFMSAFIRAKKPAVTV"
     gene            31652..32746
                     /gene="arsB"
                     /locus_tag="PKK81_00665"
     CDS             31652..32746
                     /gene="arsB"
                     /locus_tag="PKK81_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNV31406.1"
                     /translation="MELALSVDEPQVATKRLSFFERYLTLWVALCMVAGVLIGQAVPG
                     LVSSLRGAEIGQGSHINLPIAVLIWLMIVPMMMKVEFASVRDVGKRPVGLLITLFVNW
                     VVKPFSMAFFAWVFFRYVFSAWISPAEADQYIAGTIILAAAPCTAMVFVWSYLTDGDP
                     AYTLVQVAVNDLIMLVLFVPIVGLLVSGASSLAVPFDVLLYSVAIFIVIPLIVGAGLR
                     LWLVRRYGLRWMEEQFLPRFAPVTIAALLLTLVCIFAFQSQNILGKTVHVVLIAVPIL
                     LQVYMNSALAYGLMHRWRVPYAIAAPGALIGASNFFELAVATAIALFGPESGAALATV
                     VGVLVEVPVMLSVCSLCNKTRHWFNQEVGT"
     gene            32743..33183
                     /locus_tag="PKK81_00670"
     CDS             32743..33183
                     /locus_tag="PKK81_00670"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNV31407.1"
                     /translation="MKARVLFLCTGNSARSQMAEGWLRHLAGDRFEVFSAGTHPVGLN
                     SGSVEAMAEVGIDIAGHRSKHVSEFLTQPFDYVITVCDRAKESCPTWPGPTHLLHWSF
                     DDPAAVTDSDEAHRQLFRRVRDEILGQIKGFLAQEAKSLVSGPC"
     gene            complement(33180..34217)
                     /gene="arsS"
                     /locus_tag="PKK81_00675"
     CDS             complement(33180..34217)
                     /gene="arsS"
                     /locus_tag="PKK81_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNV31408.1"
                     /translation="MGLSLLARRDPLAAASEQLRLLAQTTACQPFETALNAVELYPLH
                     ATGITTLQLNLGKLCNQTCRHCHVDAGPDRTEVMSKETLDLCLQALARTDIPTVDITG
                     GAPELNPHFRWVVEQARLLGRQVLDRCNLSVLLLPSQADLGEFLAQHRVEVIASLPSY
                     QATQTDAQRGEGIFDKSMEALRLLNRLGYGKEGSGLTLNLVYNPVGAFLPPKQEGIEA
                     KFRKELASRHGVSFTRLYTITNMPISRFLEFLIESGNYEGYMERLAAAFNPAAAAGVM
                     CRYTLSVGWDGTLYDCDFNQMLELPVSQGTPQHIRDFDPARLHHRQIVTRNHCYGCTA
                     GSGSSCGGAVT"
     gene            complement(34248..35435)
                     /locus_tag="PKK81_00680"
     CDS             complement(34248..35435)
                     /locus_tag="PKK81_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV31409.1"
                     /translation="MAIDDITQKVSDRYARAAATGEQMCCPTGYDFADLKSFVPEEVL
                     TISYGCGTPAGLNTVQSGETVLDIGSGGGIDCFEAARRVGPTGRVIGIDMTDTMLEIA
                     RRNAPIVAGNLGYATSNIEFRKGMADAMPVEDASVDLIISNCVINLAPDKRKVFREMY
                     RVIKPGGRFTISDIVSDQVVPQYLVHDAAKWGDCLSGALQVQDYIGGMVDAGFRAVHQ
                     IKSTPWQSIDGIHFLSVTLTGYKFPAIADEKTSSYATLRGPFSSVTDELGQRYERGVP
                     QSIDAQRAQLLQSEPLRSLFLLGPTPVTLAATDPRWCAIHPEQKPCVWQGAYAILTGP
                     IISAEDDDHHQYYRGVPLEICSKTLQVLTQEAYRPHFTIYQRASAGVDGTEVACSPTG
                     GCC"
     gene            complement(35569..36045)
                     /locus_tag="PKK81_00685"
     CDS             complement(35569..36045)
                     /locus_tag="PKK81_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HNV31410.1"
                     /translation="MSQRMASLSLILGLALTVAACNTVGSGPAGTTAGEKSLYDRLGG
                     KTAITAVVDDFVGRVAADTRINGKFANANIPRLKSMLVDQICQASGGPCTYTGRDMKS
                     THAGMGVSSGDFDALVGDLVATLNKFKVPEREKNELLGALGPMKGDIVEKPMASMQ"
     gene            36269..36874
                     /locus_tag="PKK81_00690"
     CDS             36269..36874
                     /locus_tag="PKK81_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV31411.1"
                     /translation="MIHSQAVAQTPLMYEQEWAGLLARIAAGDQPALAEFYDASSAKV
                     FGLVMKILADRTVAEEVTMDVYTQVWRRASSYDAERGTPGSWLMTLAKTRAIDRFRSS
                     YLERGRQVPLDHAAEVPGDRATPEQYSAGLERQRLVQEAMASLSAEQRQAIALAYYWG
                     LSQSEIADRLQLPLGTVKTRMRLGMIRLREVLAPHGEGVRS"
     gene            36871..37455
                     /locus_tag="PKK81_00695"
     CDS             36871..37455
                     /locus_tag="PKK81_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV31412.1"
                     /translation="MTDPRHKEDMSEHAALYALEALGDDEQRQFKQALETAPPEVWQQ
                     VAAFQEVVQELAFSGPAIAPPASLKAKLMARIAQEPQERPGGGTFTFVRSGQGAWRDL
                     GSGVSMKLLFNDSAGARATMLLRLAPGGKLLAHRHRQVEEFYVLEGSCICAGEFLQAG
                     DYHRAEAGSVHPITSSEQGCLALVMTSTQNEPAG"
     gene            complement(37461..37838)
                     /locus_tag="PKK81_00700"
     CDS             complement(37461..37838)
                     /locus_tag="PKK81_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HNV31413.1"
                     /translation="MWYRIGADLVLLLHLAFVLFVVTGGLLLLKWPRLAWLHLPAAIW
                     GAIVEYTGWICPLTPIEHTLRAMAGESAYGSDFIGHYLLPLMYPAGLTSNIQILLGTA
                     VVLVNVTIYWRVFGRPRKGQQQE"
     gene            complement(37910..38317)
                     /locus_tag="PKK81_00705"
     CDS             complement(37910..38317)
                     /locus_tag="PKK81_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNV31414.1"
                     /translation="MSGGPPNQSRRMRHDSFKDFVLDQLGEMPELTGKAMFGGYGLYQ
                     RATFFGIIHKGRLYFKTNDVTQALYRARGMHPFRPGAKQTLRQYYEVPVDILEDPQAL
                     AAWARSAVTLPTAQLPLPFTTAPPQGYDLPAQM"
     gene            complement(38632..39105)
                     /locus_tag="PKK81_00710"
     CDS             complement(38632..39105)
                     /locus_tag="PKK81_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNV31415.1"
                     /translation="MSTLIRWDPFRVQWNPLKERDELEHRLSTLLGHRASTGNGGKEA
                     LTVAEWSPLVDIVEDENEYRIKAELPAMKKEDVRLTVDNGVLTISGERKYEQEEKREK
                     HHRIERAYGSFVRSFSLPEDADGSKVTADYKDGVLHMHLPKSEKAKPKSIEIKVS"
     gene            complement(39162..39383)
                     /locus_tag="PKK81_00715"
     CDS             complement(39162..39383)
                     /locus_tag="PKK81_00715"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNV31416.1"
                     /translation="METNVSRTTPQRAGTAHTSPSHDKAAHAGQAAKGTAAIALRAYE
                     LYEKRGRKDGQALEDWLNAERQLAAAAGH"
     gene            complement(39510..40067)
                     /locus_tag="PKK81_00720"
     CDS             complement(39510..40067)
                     /locus_tag="PKK81_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNV31417.1"
                     /translation="MTTRNVLTLCAALVLGGAMITGSAHAIGKADEKTPINDSWITAK
                     TKIALFADSRVKGSEINVETAQGAVMIRGKVDSDAAKQAAEGIAKGIDGVKTVKNDLQ
                     VVAPSKREATDDKDDAITARVHEHMEKDAHLKKAGIHAQTNAGVVSLSGEVQDLATSA
                     EASWSAWQVPGVKAVKNDLTVKEKA"
     gene            complement(40130..40357)
                     /locus_tag="PKK81_00725"
     CDS             complement(40130..40357)
                     /locus_tag="PKK81_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNV31418.1"
                     /translation="MNADQLKGKWLQFKGDLKQQWGKFTEDDLQQIAGNYDTFVGKVQ
                     ERYGEKKAELMKWASHWHEQAKPDAEGTKAP"
     gene            complement(40452..40619)
                     /locus_tag="PKK81_00730"
     CDS             complement(40452..40619)
                     /locus_tag="PKK81_00730"
                     /inference="COORDINATES: protein motif:HMM:NF018715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNV31419.1"
                     /translation="MLYYALMFLVVGLIANALNLLGVSAVAVQMSWILFLIGVVLLVV
                     HLVSGRTPRTT"
     gene            41036..41314
                     /locus_tag="PKK81_00735"
     CDS             41036..41314
                     /locus_tag="PKK81_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31420.1"
                     /translation="MRTFIIAAIAGFCLLTGLSFSAGAGETKAKIEKGKGELKGDLEE
                     MKGETKAMKEELKGNDTKAEMERAKGKLKGAGERAKGKMKELKEKATD"
     gene            complement(41390..42247)
                     /locus_tag="PKK81_00740"
     CDS             complement(41390..42247)
                     /locus_tag="PKK81_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV31421.1"
                     /translation="MPSQTPEAVIEAQRQDWNRVAAGWDKWDQFFNRNMAFINHRLVA
                     DARVRPGLRVLDLGSGTGYPALLAGEVVGSDGTVVGIDLAESMLAVATRKAKTLGLEH
                     VTFRTGDVTSLPFETGSIDAVISRFCLMFLPEIPKAAAEIARVLKPGGYVAAAVWSAP
                     DKNPFIRIPMDVIKTITPLPPPDPEAPGIFRLAKPGDLAGMFERAGLKPLDDEEFTAE
                     VTYATAEEFFRGLMDIAAPIQNLFAKLTQEQQAEAERGIVKAVNEYRGPQGVSLPIAV
                     RIVSARKPQ"
     gene            complement(42285..42788)
                     /locus_tag="PKK81_00745"
     CDS             complement(42285..42788)
                     /locus_tag="PKK81_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31422.1"
                     /translation="MPRGILRALCGLVLLFVPLFAQAADTPDTTLSPLKFLVGEWKGA
                     DAEGKAHKIAFALSSGGTSLTETLTPPDSPPMTTMYYSDGDQLMLTHYCSLNNQPRMR
                     ASAIKEGDKTITFAFVDATNLKNPTDVHMRQLTIDVKDHDHFTQTWTLSKAGKDVPKV
                     FTFERVK"
     gene            43362..43958
                     /locus_tag="PKK81_00750"
     CDS             43362..43958
                     /locus_tag="PKK81_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HNV31423.1"
                     /translation="MAHHHSATITVPRHAEPRGHDHHTNGHDCVQAHGDHRGRHQPHE
                     HQEANHQASSLDRTALMATLHCLSGCTIGEVLGMAIGTALGWSNWPTVGLAVALAFLF
                     GYAMTLYPLRRAGMAWGTALGLAFASDTLSMTTMELVDNAIMVMVPGAMDAGLPDPLF
                     WGSLVLSLLLAGVAAFPVNRWLIARGKGHALVHGQHCH"
     gene            44353..45300
                     /locus_tag="PKK81_00755"
     CDS             44353..45300
                     /locus_tag="PKK81_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31424.1"
                     /translation="MTIGKYMCMLAIVATAMVAFALAGCSQFEPRDKRFYYRALWNFS
                     LREDLAELDSEFNGVDFGHSNLYENLLLTGGQDVPAIEERARKQTIAFIATKPRLNPN
                     EEAIAPTYMKLAWRAQNTFDEAHALHRATYDIAVSDEPEKDRAIRNVLAYYKDSAYAI
                     TSKRLDHHRLDQFPYSKAFRTRFPLFNATIWSYHYLQVAVYDPLQAARDLAAKTQAVR
                     PILATYRRYLEQPPVQWTFMPLTAELSPQFAARYPELANIFDNLHMLHDNISDILTSE
                     RLPTWEAKRAEIYRVLNSYYLASADATNPMIVQGQEHHH"
     gene            45297..46082
                     /locus_tag="PKK81_00760"
     CDS             45297..46082
                     /locus_tag="PKK81_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNV31425.1"
                     /translation="MKLRDKRFLARLLGSALALVTTPLFALDHDNLDPNRPIGMEDAY
                     AIPKGEIGLEGGVRFNDRREGRTQVTFQPQIIYGAFANTQIEIQGDLFTDPHSIVGAN
                     KSGDLHLGVLYNFNTETLMLPALAVRVEADLPTGVNSKGVDTQLTGILTRSFGRLRVH
                     LNAGYTVIGSPQGQERPGAYRAVAAVSYPLGYPTSFRDTLIVSVYTRQSDLRGQQNHT
                     GIEVGLRHQLTSRVVLDGGLGTEFVGPADRAALLGTMGVSVGF"
     gene            46374..46592
                     /locus_tag="PKK81_00765"
     CDS             46374..46592
                     /locus_tag="PKK81_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNV31426.1"
                     /translation="MAVQKLSSKNQIVLPKEARQAMGVRGGDELLIVVKDDLTLVMPK
                     PKRYAQTLRGLAKGTYPSGYLKRERRSW"
     assembly_gap    46682..46781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <46782..47144
                     /locus_tag="PKK81_00770"
     CDS             <46782..47144
                     /locus_tag="PKK81_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV31427.1"
                     /translation="PRYGAWCASLFDLIERGLNPAVTSTVTLLELLVQPYRDQKDELA
                     QRIFALAGTYPRLEWVSVTMNVADRAAELRARYRLSTPDAIQLATAILHKAVRFYGND
                     RGLRKVKEIECVLIDELI"
     gene            complement(47344..48054)
                     /locus_tag="PKK81_00775"
     CDS             complement(47344..48054)
                     /locus_tag="PKK81_00775"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNV31428.1"
                     /translation="MNGFDTAVLGFFNQFAQRSWAVDSLIMTISDSDALKGYLSLPLI
                     WWAWFRAPHREDHRQTVLLTILAAFAAMAIARVLQLVLPYRARPVHTAGLDFTLPYTM
                     PADFLAGWSSFPSDHASLFFALAAGLWLVSPLVGAFAFLHAAVIVSIPRIYVGLHFPT
                     DILSGACLGVTAVAVIWKWRAVFVKLVEPALAWSKTSPHLFYPAFFILTSELAHLFKD
                     SRWLAESLWHMAKLSLAR"
     gene            48388..49281
                     /locus_tag="PKK81_00780"
     CDS             48388..49281
                     /locus_tag="PKK81_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV31429.1"
                     /translation="MGRAGRIARGIMVLVSVLLVAACGDSFNDAYCEANNHRNQQTVE
                     HLTNVLEQKRNQPGLYLARARCHYFLGAYAQALQDASEVIRRLPNKADAYLLRAKAGK
                     MLGQIPQALQDYSAAIKFQPTAEAHYGRGLTYVREYQGKDREALEDFTAAIRLDPKHV
                     GAHAFRAQIYSRHEQFQHALADSETVLKLDPTTPNAYCNVGFAHYALGHDAEGRKFLT
                     TCYQKDPDPQTRGYYETEVNRVLYARKPKRNSGGNYVAEGGVKTPYDREMERQQNVYE
                     SLRNSGFNDRAEACRTDGSKC"
BASE COUNT         9630 a        13375 c        15850 g        10408 t
ORIGIN      
        1 ggcgccgacg gcgggcagat cttcggcata cccggcgaag gtctggccgg cttgcgtcag
       61 ggtgctatga agattcgggg ccgtcaacgc gagcgggcag gtgttcccgt tgaccccttc
      121 gatggatccg gcaaacaagg caatatagtt gggttgactg ggatgggtga tgccgtaaga
      181 gttggtcagc agcgcgcctt tgcgggccag ggagttgagg taaggagcta aaggcgagtc
      241 gatgatctga gcgaacgaat ggttctcctc gatcacgatc acaatatggt cgggcttggg
      301 caacggcgtc tgggcggaaa cgggttcgat cccgaagaag agagccatcc acagtgtgag
      361 tatggtcagg acgcgtgtgc acatgtgaag cccctccggg ttacacgggc agttcgctga
      421 gtcgcagggt atacagataa tgatagaggc cccgctgctc cagcagtgcg gcatgggtcc
      481 cttcttccac gattttccct ttattcagga ccaggatgcg atcggcgcgc tgaatggtgc
      541 tgagccgatg cgccacgacg aaggtggtgc ggttgaccat caggcgctcc agcgcttcct
      601 gcaccagccg ttcggactcc gtatccagag cggaggtggc ctcgtcgagc aggagaattc
      661 tgggattctt cagaatggct cgcgcaatgg cgacacgttg gcgttgaccg cctgagagat
      721 tgatgccctt ttctccgacg atcgtctgat agccgtccgg aaatcccacg atgaactcgt
      781 gggcatgcgc ggccttgctg gcggcaatca tctcttcttc gctcgcgtct tcccgcccgt
      841 aacgaatgtt gtcccggatg ggcccgccga acaggatcgt gtcttgcggt accagggcga
      901 tctggcggta caggctgtcg agccggacgt ctttgacatc gtggccgtcc accgtgagcc
      961 gtccggcggt cgggtcgtag aatcggtgca agaggttcat gatcgtcgtc ttgcctgacc
     1021 cggtgggtcc gacgatcgca atcaactcgc cgggcttggc ctcgaaggag aggtccgaga
     1081 ggatcggctg ccggggatcg taggcaaagc tgatgtgctc cgcgcgcaca tgaccacgga
     1141 tgggcggcag atcgacggcg cccggggcat cggcgatttc ggcatgggtg tcgagaatct
     1201 caaagacgcg ctgcatggcc ccttgggctt ccttgatctg cgcgaacact cgggctgccg
     1261 atccgaacgg tccgatcaga atgcctgcaa acagcacgaa ggcaaacagg tcgccggggg
     1321 agaccgtgcc gtcgatgacc tggcgccccc cataccacaa gacgagggcg gcggcggcaa
     1381 aggtgacgaa gatgatcgtc ggcacgaacc aggccatgat ggttgcacgg cgcagtgaca
     1441 gctccatcgc ggactggacc tgagccacga agcgttcttc ttcccgtttc gtttggacga
     1501 aggatttcac gacgcggatc cctgcaatga cttcttcgac cagcgtgctg acggaggcgg
     1561 tttggtcctg gattttggtg gacagcgccc ggagtcgccg cccgaacagt ttggccacga
     1621 cgaccagcat cgggagcagc accagaatga gcaggcagag ttgccagttc atggcgaaca
     1681 ggaagcccgc gccgccgatg aaggtgacga tttgtttggc ggcgtcgatg ggcgtatcgg
     1741 tcacgacgtt ttggatgacg gtgacatcgt tcatgaggcg ggacatcaat tcgccggttc
     1801 ggcgcttcga gaagaagttg accgacagcg tctcgagatg tgcgaagaga tggatccgga
     1861 aatcggccat cacccgttgg gagacccagg cggtgagata actgtgtccc atcgaacata
     1921 acccctggat caggaccaag cccaacaaga gcaggatcat ttctgtcatg cgtgcggcat
     1981 cgtgctggac ggtgatgacg tcccaaaggg tgccgcctaa gcgaaggagg gcaaggttga
     2041 tggcggcgac agccataacc atgacggccg ccgccatcat tcgagggacg tagggcttga
     2101 ggaaggggta gaatcggtta aacgagaaca tgggtgttag gacaatcgtt tcagagaggg
     2161 caggcctgta gcgtatcacc gttggtggcg gcgatacgca tacgacatga ccttcgtggt
     2221 gaggtgagat cgcgctagag ctgagcgatg gactcgatca agttcttatt gatcgccaca
     2281 aagtccgacc gatccttatc gttgatcaac acgtcagtca ggctgatgaa cagccgctgc
     2341 tcttcgttga tcgcgtcgga cacacgattg cgcatgggcg ggatgaggaa atccccgacg
     2401 tacacacagt tcaccgttcg aacgcggatg cgaacttttt tgccttcggg cacggatccc
     2461 tccgtccatt atgtcgtgat gggctcagct cttgcgacgc aagaatttct gacgccggcg
     2521 caacttttta tacttgtgct tgcgcatctt cttccggcgt ttcttgagga cactcgccat
     2581 acattcaatc tccaactgct aggcgcggga gtctagtggg tttcagtcca aaatgcaagc
     2641 caagattatg acttgttgca tgtcgatagg ccgcataacc gtatgcaggc ggcgattgaa
     2701 cgtggggtga gccactcagc ttgacctctt tccgccaagg ttcctatact gcgccatgat
     2761 tgaatcttgc ccgccgattt ctcttcgtct cggtcgcgtt ttccttgccc tgctgctgat
     2821 catgactgtg ggttgtgcag gaaaaaccct tcagtataag gacgatcacg accgtattct
     2881 ccgtatcgac caggccgtgg agttgctccg caaatcctac gtagagcggg accgtagtgc
     2941 gctggaggcg ttgctcctcc cgtcgggaaa tctggatcag gtgcaacgtg agattcacgc
     3001 cgacttcgat acctttcgcg acattcagtt ggagttttcc atcgagcgca tcttaatcga
     3061 aggggacaat atcgatgtgt ttgtccattg gcagggccag tggaaacggg accctgccga
     3121 ggtgggcacg cgccagcgtg gccacgggcg gttggcctgg gtcggcacac agtccattct
     3181 gcttcgggaa gctcagggcg atctgccctt cggcatgggc gcgcgcgcca gcgtgggcga
     3241 agacccggca cgcgggcaag ccaagccata gccatgtctg ccaggttgtc ctcgtccaaa
     3301 attgaaacag actttctcgt ggtcggaagc ggcgtcgcgg gtttgcgcgc ggcgatcgaa
     3361 ctcagccggg ccgggcgcgt gttgatgttg accaaggggc atcctctgga aagtaattcc
     3421 atgtatgccc agggcggcgt cgcggtggcg ttgagcgagg aagatgatgt cggcagccat
     3481 ttgaccgata cgctgaaggc cggccacggg ttgtgtcgac gggaagccgt gcgtgtgttg
     3541 gtcgaagaag ggccggaacg gatccaggaa ctgatcgcct ggggcgcgaa gttcgacaag
     3601 atcggaaagc gcttcgcatt cacccgtgag gcggcccaca gccggagccg cattctgcgc
     3661 gcgcgaggcg atgccacggg caacgagatg gtgcgcgccc tcatgagcta tgccgcgagg
     3721 cagccgcgca ttcaacgatt ggatcggcga ttcacggtcg acctggcggt cgtcgatgga
     3781 cgctgttgcg gcgctattgt gctgaatgag atgaccggcg aacgcacggt gttaccggcc
     3841 agtgccgtgg tgttgtcgac cggtggcgcc gggcaggtct atgcgcggac gacgaatccg
     3901 ggcaacgcga cgggagacgg catggcgatg gccctccggg ccggggcgtt gttgatggat
     3961 atggagtttg tgcaatttca tcccacgtcc ctgtatctgc cgtcgagccc gccgtttctc
     4021 ctgtcggagg ccatccgcgg ggaaggcggc cagcttcgga atatcaaagg cgagttgttc
     4081 atgcatcgct accatccgga cggcgcgctg gcgccgcgcg atgtggtgtc gcgggcgatc
     4141 tggtccgaaa tggcggcgac tcgcgcgcgt catgtctatc tggatgtgac gcatctgggc
     4201 gcggcctttg tgaagcggcg attccccacc atctatgcga cctgcctgcg gtacgacatc
     4261 gacatgaccg aagaatggat ccctgtctcg cccagcgcgc attacatgat gggcggtgtc
     4321 tggacggatg tgcagggagc gacgacgttg cccggcttgt gtgcggccgg cgaggtcgcc
     4381 tgcagcggtg tgcatggcgc gaatcgtctg gcgagcaatt cgctgttgga aggtttggtc
     4441 ttcggcatgc gggctgcgca atctgcggtg gcccatgcgg gacgattccc ggggccggtg
     4501 cgcatgcccc acctggaaga actcgaacag ggccggccga cggacttcga cgatccggag
     4561 aagttgcgaa gctcactgcg tcgcttgatg tgggggaagg tgggactcgt gcgctcaggc
     4621 gattcgttga tcagtgcctc cgcgcaactg gtgcgctgga tgcgcctgtt gaccaagcca
     4681 tttgcctcgc gagcggccct ggaggtcaag aacatggtgc aggtcgcccg ctgtatcact
     4741 gacgcggcat tatggcggga gaatagtgtc ggggctcact accgttcaga ttttccggag
     4801 cccaatcggc ctggatggcg gcaacacagc cagttgctgt ttacggacgg tcagacgagc
     4861 gggtcggcag cgaaggagcg agcgctcgtg ctttcgcccc cagcgcctgt gcgcggtaag
     4921 cccgggaaga agaaagccgg tcgttcgtaa ggaaggtgcg gtagtaccga agcacttttt
     4981 ggacatactg cctggtttcc ggaatagggg gaagcccttg gtaacgctcc acggtatgtt
     5041 cgccggcatt gtatgccgct aaggccagcg gaagatttcc gttaaaccgg tccagcaact
     5101 gtcgcagata tttcgtcccg ccgccgatat tctcatccgg attgtaggaa tcccgcacgt
     5161 ccaagcgcac cgctgtttgc ggcatcagtt gcatcagtcc gacggcgccg gcgcgtgaca
     5221 ccgcctgcgg gatgaagtcg gattccgttt tgatcacggc gcgaatcagc gccggatgga
     5281 ggcgatgggc tctggaatgt cgggcaatca ccggctccaa ttccccgtcc gagatcaccg
     5341 cgcgcgagcg aggcagttca gcaatcactc gtctgtatcg aggatcgttc ggcacgttgg
     5401 tgagagaaat ggtgccgttg gcatcgatgt actgatagac gtcagcctgg ctggggaggg
     5461 cattcaggag cagtgccgcg gcaactgaga agagggctgc tgtgcggctg aaatgggcca
     5521 tctggattgt ggctcccgtg tacgaaccga atcccattcg gatgtttcaa ccgtagcatc
     5581 ggaccctcga cctgtcaaaa aactccgtcc ctggctagcc cgattgtttc ctcgtaacca
     5641 attgatacta tggatatgta ttgccatatt cgttacgagg gcggctcaag aaacctcgtc
     5701 cggctgtcgg cgaatcgcgt ctgcagccga cgggtcagcg agcccggccg cccatcgccg
     5761 atggcgaacg aatcgaccct attgaccgga atgatttcca tgctggtatt ggtcagaaag
     5821 cattcctccg cccgctggag gtcagcgagg tgatagtgac cttcccggac gggtacccct
     5881 tcctcttgcg cgagggtcat gacgatggtt ctcgtgatgc cgtccaagat gccgcagtcc
     5941 agggctgggg tatggagggt tttgccggag atgaaaaaga gattgctgac ggtgcattcg
     6001 gtcagctcgt ctttccaatt caagaacagg ctgtcgaagg tgccggccgc aatcgactca
     6061 cgcttggcga gaatgttgtt gaggaaattg gtggatttaa tctgaggaga cagcgcttcg
     6121 ggcagattcc gcctggtgtt ggcgacggtc agcgacacac catcgcgtga gagtacggga
     6181 ggagaggggt ggagcggctt tgccatgatg accaccgtgg cccgcgggca cagaccggga
     6241 tccaggccga tttcccctgc gccgcgtgag acggtgatcc ggaggtacgc atccgtctgt
     6301 tcgttgccga ctccgttccg gcgcatcgct tcgtggagca actccggcca gtgcgactcc
     6361 gggataggaa tgtccaggcc gatggcttcg gcggagcgcc ggagccgggc caggtgttgg
     6421 tcgcgcatga agatgcgggt tccataggag cgaagggttt cgtagactcc atcaccgtag
     6481 agaaacccat ggtcgaacac ggagacgagg gcttcttctt tgcgtacaaa acgatcattg
     6541 agaaacaccc acatcagcgg ccagcctcca gtattttgaa aaatgcgccc gctttggcga
     6601 gcgtttcatc atattcgcgg tcgggatccg agtcggccac aatgccggca ccgacttgca
     6661 gataccctcg gttcttggtc agcacgagcg tacgaatgag aatgttcagg tccaggtcgc
     6721 cgttccaact gaaataaccc aacgcccccg tgtagagtcc gcgccgcacc ggctccagtt
     6781 cttcgatgat ttccatgcaa cggagtttag ggacaccggt gatggttccg ccggggaaca
     6841 cggctttcac aaggtcgagg ggtgagacgc ccggttgcag ggtgccgacc acgtctgaca
     6901 ccaggtggct gacatgcgaa tactgttcga tggtcatgaa ctcatcgacc cgcaccgagc
     6961 cgtagcggca gacctttcca aggtcgttgc gttcgagatc caccagcatc acatgttcgg
     7021 cgcgttcctt aggattggca agcaactccg cgcgcagaag ctggtcctgt tgcaaaccgg
     7081 tgcctcgcgg cctggtgccg gcgatgggtc tggtgctggc ctgcgagccg gtcagccgca
     7141 cgagccgctc aggggagctg ctgacgaggc tgaggtcggc gaatcggacg agccccgcga
     7201 acggcgcagg gttgatccgg cgaaggcggc gatagagctc ggttgagtat ccctcgaatc
     7261 cggtcggtga agcggcggcg tgcagatcga gggtgaagcg gtgcgacaga ttggcctgat
     7321 agatatcacc ggcggcgata tagtcctggc aacgccgcac acgagttttg taggcctcgc
     7381 gcgattggct gggcacaaag gtggtttgag tgggccaggg cgaggaggta agaggcgtga
     7441 ccgggctcgt gaggcgggct tccagctcag ccagccggtc acagccttcg cgatagagtt
     7501 tctcccgcgg ctccgcctga aatcgggaca gaggggggca atacatcagg tagagctgtc
     7561 gcgtatggtg atcgatcgcg gccacgacgt cgaaaaaggc catgtgcagt tcagggagat
     7621 ggagatcgtc ggcggccagg gtcggaagta attcgaacga acgaacgagg tcgtagctga
     7681 ggtatccgac tgcgccgcca taaaacggcg ggaggccttc cggtttggta atggtcgatt
     7741 cggcgagcgc ttgttggagg gcctgaatgc cggaaccttg atagcgccgg atctgtcccc
     7801 cggtctcgat ctggtattcc tgcgcgcggc ttgtcagggt gagatagggg tcgcgtccga
     7861 aaaatgaata gcgcgcggtg gtgtcggtgc cgttggcgct ttccaggaga aacgagggac
     7921 gttcggccgg ggccagccga cagtacaact cgaacgcgtc gacatccggt tgtggacgca
     7981 cgaccaccag cggttgcggc ggtccgctca ggaatgcctg gtgagaggaa aagctcatga
     8041 acctgcgttt cagaggggtg ccatacccga ggaaatgact ataccgcatc gagcggccgg
     8101 gtcataggtg gccaacttct tgacaagccc ccaacgaatt tgcttgaatg gcgcgcccgc
     8161 cgatgggtgc actctctccg attgcgctga ggagtgccgc acgtgagcat ctgatgcccc
     8221 cctctcagga tccgttatat gcggggctcg ggcaggctgt ccggatcggg acggaactgc
     8281 ttgctgcgtt gatcgtcggg ggaggactgg gttgggtcgt cgacacctat ctgctcgatt
     8341 ctaatccatg gggattggtg gtggggttag ggttgggggc cgcagccggt gtacggagcg
     8401 cctatcggtc tgcgcaacga tggcagagtt aaccagaatc gtctgactga taaaggatca
     8461 tcgtggaaga aagtccgtta catgcgtttg aactgcacga tctgattccc ttggttccgg
     8521 ccggggtgga tatttccatc aacaaggccg tgattctgat gtgggtgatc gtcggcctgg
     8581 tggcgttcct gatgatctcg gctgcggcgg cccgtaagtt ggttccgggc aaattgcaga
     8641 acatggccga attaatcgtc gagttcattc gcgggattat tttggacacc atgggcgagc
     8701 cggggatgaa atatttcccc ctgatcgcca cgttgttcct ctttattctc tttgccaatt
     8761 tgatcgggtt gattcccggt tcctataccg tcaccagtca gatcattgtg acggcagtct
     8821 ttgctgtagg ggtttacggg ctgagtattg tgctcggctt ttcccttcat ggcatgaagt
     8881 ttctcggcat tctcgtgccg cccgggacac cgggttggct cttgccgttg atgattccga
     8941 ttgaattgat cagtcagttg gcgcgaccca tttccctggc cgtccgtctg ttcgcgaata
     9001 tgactgccgg ccacgtcatt ctgggagtct tgttcggtct ggcgatcagc ggtgggttgc
     9061 tgatcgggtg gctgccattc gcgttcacca tcgccatgaa cggactcgaa gttggtatcg
     9121 cgtttattca agcctatatt tttaccgtgc tgagctgtgt gtatctgggt gatgcggtta
     9181 ccctgcacgg tcatagcgag cacgcacact aggtcgagtt gtttacataa gggaggagag
     9241 gacaaacaat ggatgcagca gcagcagcgt tggtgggtat gggattggcg gcggcaggat
     9301 ttgccggtgc cggtgtgggc atcgggtata tcttcgggaa aatgatcgag gccgtggccc
     9361 ggcagccgga agcggaaggc cgcgtcggca agtacatgtg gatcgggttc gcgttggtcg
     9421 aagccattgc actgtacggg ctggtcattg cgttcatcat catgggcttg cgtaagtagt
     9481 cgtttcgacg gctgagccct gatgctgggg cctattcccg gctcagaacc tcaactcatt
     9541 tgggtaatag actatgcctc aatttgaatc acatttcttt tcgtccttga tcttctggga
     9601 gattctctcc tttgggatcc tgttcttcct gctctacaag tatgcatttc ccagcctgtt
     9661 gggcatgctg gaagagcgcg agaaaaagat taaggacagt ctcgatcagg cggaacgcca
     9721 ccgttccgaa gccgagcgca ggctgaagga atacgaagcc aagctggcga cggcggccaa
     9781 agacgcagag gcgctcctgg cacaggccaa agaacgcgcc caacgcctga tggaagagaa
     9841 cgaacagcgc atgacggcgg atgcggaacg catcaagggt gacgcgactc gcgaaatcga
     9901 gaccgagcgc aggaaggcca tccaggacat ccgttcgcaa acgaccgact tggcgatgat
     9961 ggtggctgag aaagttgtcg ggcgggcact gacggaaggc gatcatcgtc gtctggcgga
    10021 cgaagccctc gatgccctcg caaagagcta ccagagccgg aattagcctc tcttacgccg
    10081 ggggggtcag actcacgccc ccctggcggt atccttccaa atcaaccgtc acgaagcgat
    10141 agccgagttt cttcagggtt tcggccagcc gttcccggcg tcccggctgc aacaacccca
    10201 gcagttcttc tacggccagt tctattctgg cgatctcccc atggtctcgc acacgatatt
    10261 ggcgaaagcc ttcttgtgcg agagcatctt ccgcacgctc cacgcgtgaa agtgtgcttc
    10321 tcgtgatcgt gatgccgcgt ggtatgcggg aagagaggca ggcggctgcc ggcttatccc
    10381 aattggagag gtgtagctct ctcgctacgt cgcggatgtc ggccttcgag aatccggctt
    10441 cgagcaacgg gctacggacg cctcgctcgc gagcggcctt gaggccggga cggtcgtctc
    10501 ccaggtcatc cacgtttgtg ccatcgacaa tgtatccatc cgcatattcg cttcggagcg
    10561 tttcgaggag tgagtacaag tcagtcttgc agtggtaaca gcgtgtggca tcattgagga
    10621 cgaattcagg aatttggagt tgatccgttt caacgatgcg atggcgtgcg ccgatctctg
    10681 cggcgatttt ctgcgtcgcg tccaactcac tggccggaag ggtgggagag acagccgtaa
    10741 tgccgacggc ctgggctccc agttcatcat gcgccaattt gaggacgagg gaactgtcga
    10801 tcccgccgga gaaggccacg atgaccgagc ccatctctcg aaggatctgt cgtgcttgag
    10861 agactttatc gatcagcggc gtcgtggaaa ccatgggatt ctaggtctgc gcggcccggc
    10921 tgcgggagag tggccggcta ttgtttgact ttggccttcg cgatcgatcc gacgacgacc
    10981 aaggcgtaat gttgggggtc gagatactgc ttggcgacgc gtaggacgtc gtccttcgtc
    11041 accttttcga tggctttagg atactgcgtg aagtagtcca acccgaggtt gtagagttcg
    11101 acctgggcga ggacgtttgc cagtttggcg ctggaatcga cccgcagcgg gaagctgccg
    11161 acgatgaagg acttggcctc attgagttcc tgatccgtga ccggggcatc gcgcatccct
    11221 ttgatttccg tgaggacgcc cgcgatggcc tggtttgtga cgtcggttcg ggtctgcagg
    11281 ctgataaaaa aggctcccgg catcaagcgg gtgtcgaact ggctcatgat tccataggcc
    11341 agtccctgtt tgtcgcgaat ggaatccatg aggcgggagg agaacccacc ggcgcccagg
    11401 atatagttca tgactgtgac ggcatagtag tcgggattgg tccggctgat gcccgtgtgc
    11461 ccaaggacga tcgtcgactg cgtcagatcc ttctcgatga gctggaccat tttacgttcg
    11521 atcgaagccg gcttcttgat ctggtagggc gagggggggc cctttttcca ggagccgaaa
    11581 tgggtctgga ccagagcggc ggcctgatcc tgtgtcaggt ctcccacgat gaccaggatc
    11641 gtttgattcg gcaggtattc cctggcgtga aattgctgga tgtccgcgac cgtaattttg
    11701 ttgagcgtct cttccgtgcc atgggccggc cagctatagg gatggccgtg aaaaatcaat
    11761 tggtggaagg ccttcatggc cacattcccg ggatcatcgt catcgctgac gatttcgccg
    11821 aggatctgcg tgcggacccg ttcgaattcc tgtttgtgga acgcaggatg ttggagcatg
    11881 tcggccagaa gcgcaaagcc gaggtctgca tccttcttga gcacgcgggt ggaggcggtg
    11941 gtgaaatcct ctgccgcatg ggcttccagc gagcctccca caaaatcgat ctgttcggcg
    12001 atttgtcgag atgtccgggt cagggtgcct tcatcgagga ggctggcggt cagattggcc
    12061 agacctgctt tgtcgggtgg atcctgcgcc gatcccacct tgaccagggc gtggatttcc
    12121 acggtcggaa gaaaatgctg ttccagaaac agcacggtca tgccgttcgc ggtcacggag
    12181 cgggtgggga cgatgtccgc agcttgcgcc aaggcgaacg gaaggctgag cgccatacag
    12241 agcaggcccg ctaacagccg gcccacaggc cggcggcttc ggtgttggcg tggcagaagg
    12301 gtcgctggtt gttgcgtcat cgggatcgct cgctagtgag gttgtgccgg tgacgcggcc
    12361 tgggtttgcg gaggttgagg aatcagggtt cccgtggtgc gcatatcggc ggccaggtag
    12421 tgcgacgccg cacgctgcac gtcctgcgcg gtgacggcgc gggtacgctc cacaaactgg
    12481 ctgactttgc gccatccggc cccgatcgtt tcggcttctc ccagcagcat ggcctggcgg
    12541 aaattcgaat cctgttcaaa aatgtgggcc gcttcgatct gatttttcgc gcgctgcagt
    12601 tcgagttcgc tcggcggctc ggtttggatc cgctgaatct ctttgagcac cgcgtcttca
    12661 accgcttcaa ccttctcgcc tggtttcacc acggcataaa agtagaacaa tcccgggtcg
    12721 gcttgcatca ggccatagtc ggcgccgact gccagggcgg tcttttgttc gtacacgagg
    12781 ctttgataga gccgggcact cttgccgtga gacaaaatcg attcaagtac gttcaaggca
    12841 taggagtcgt cgctcgaata gttgggtaca cggtagccca tcatgacgaa cggcacctgc
    12901 gcttctcgct tgaggagaaa tcggcgttcg ccgtgttgcg gggcttccgt cacggccagg
    12961 ggtttggggg acggaccttt agggatcggt tcgaagagct tggcaatagt cggaaggagc
    13021 gtgtccgctt tgatgtcgcc gaccacgatc agggtggcgt tgttcggcga atagtaggtg
    13081 tcgtagtgac gctggagatc gtcgaggttc atcgcgtcga ggtccgagaa ccatccgatg
    13141 accggccagt ggtacgggtg actcatgaag gcctgtgcga agagggcttc caccagcgcg
    13201 ccttgcggat catcttcgtt gcgcaaccgg cgttcttctt tcaccacttc ccgttcggtc
    13261 ttgaattcgt tcgcatcgag aatcagccct tgcatgcggt cggcctcgag ttcgagtgcg
    13321 agcgtcacgc gatcggcagc caggttttca aagtaggcgg tgaagtcctg actggtgaag
    13381 gcattgtcca ttccgccgtt cttgcgaacg aggcgtgaga aggtgccttt ggggtactta
    13441 gccgtgccct tgaacatcat atgctcaagc atgtgcgaca acccggcccg gcccatgacc
    13501 tcgtttcgtg agcccacctt gtaccagact tgcacggtcg ccacgggagc cttcgggact
    13561 tccacgagga tcactttcat atcgttggac aaggtgtatt ccttgggctc gacggcatgg
    13621 gccggggagg tctggctcgc gaacaggatg aagcttagca atgaacaggc gaggatggag
    13681 aaccgcacag gtgctgagtt ggaagttgtg ctctggttag gcatgtacga agaacatgct
    13741 aacaacgggt ctgaatctgt gtcaaggcaa cgtgggcctc ggacgtatcg aggcgtccga
    13801 gttggccgca agcacccaac acatcgcggc cacggctgcg gcgaagatag acgtccacat
    13861 ggcctcgtcg aagggtggcc tgaaacgttt caatcgccgc gtctgagggg cgccgatagg
    13921 gattgcccgg aaacgggttg aaggcgatca ggttgacctt gcagcggagg ccacgaagca
    13981 gcttcactaa gcgggcggca tcctcggcac gatcattcac gtctgcgagg aggacgtatt
    14041 caaaggtcag acgatcacgg tccgccagcg gataggcccg acaggcctcg agcaaggcgt
    14101 cgagcgaatg gagccggttg gccgccggca tgagttgctg gcggagcgca tcggtggtcg
    14161 cattcagcga gatggcgagg ttcaccttga ggggggctac gtccttgatc cgcgaggcca
    14221 gtcctgcggt ggagatcgtg atgcggcgac ctgaaaaccc caagccccag gtgttgttgg
    14281 tgaggcgggt gacggcatcg gccacggcat ccaggttggc gaggggttcg cccatgccca
    14341 tgaagaccag gttggtcagc cggtggccct cctgcaaatg gtcctgggcg aggaggacct
    14401 gatcgaggat ctcatgcgcg cgcaggttgc gttggagtcc cagcgtaccg gtcaggcaga
    14461 atccacaatc gagcgtgcag ccgacctgcg tggacaggca gagggtgagc cggtcctcgt
    14521 cgggaatgag cacacattcg acttgattgc cgtcggccag cgtgagcaca aatttcttgg
    14581 tgccatcctg cgacgaaaag atttggaccg ccgaagtccg ttcgatactg cagctgcccg
    14641 tcaggtactc ccggtccttc tgcgagagat tgctcatctc ggcgaacgtg cgggcacgtt
    14701 cctgatagag ccagcgcagg atttgcgacg cgcgataggc cggccatccg agtgatgcga
    14761 cgaatgcggc catcccgctt tcggtcacgg ccagaagatt ggtgcgatta tcggtccgtt
    14821 ggagcatacc ggagcctagc acaggcaaaa aacttgtgac aagcaaggcg aacgtggtcg
    14881 cgtttataca gcgttcggct ataatagggc cggttcaccc tcttctcagg atgagaatgt
    14941 gaccgcactt ccttggcgga tcagcttgtc ggctcttgcc gtgtggtctg tgttgtcgag
    15001 cctgttcttg acggccacag ccgagtcacc gcgccccctg cctgttgccc ctgcaccctg
    15061 cgtctccgcg gaagattgtt tccgttccgc cgtggcgctc aatgaacgtt cgggatcgcc
    15121 cgttcaacgc gatcaaacga tgatgctgaa gatcgaccag ctgcggtcgg tgatggagct
    15181 ctatccttcc acgatctggg ccaagcgggc cggggtggtg ctgggcgtct tgcggatcga
    15241 gcgggagccg gtcgaggcgg cgaaacttct gcgtgttgtc cagccggaca tgccggtgct
    15301 cgacgactat ctccgcctct ggatcgggga atcgctgctg aagcagaacg aaccgatcca
    15361 ggcggccgaa ttgctggaga cgattcccaa gatcgtgccg gattcgagcc tgattgccaa
    15421 ggctgcctac cgaacaggcg aagcctggta tagcgccaat gtgtgcttcc gggcggtgga
    15481 ctggctggag cgggctgtgg cgcttgcgga taaggacccg gcggctccgt tggccttgtg
    15541 gcacctggcc gagtgctaca ttcgggagaa tcgactgccg gaggcgcgaa ccgcgttgaa
    15601 gcaactctgg cttcgctatc cccactcgcc tgaagcgcgg gaggcgaaag cccggttgga
    15661 tacggcactg ggcggagaat cctggacccc aacggccgaa gatcattcaa ttcgcgcaca
    15721 ggcctttctg gggctggcca tgcaagcgga ggcggtcgaa gagctgcgcc gctttctggt
    15781 gatggctccc gggcaccccc gccggtttga tgcccgcttg aagttgggcg tggcctacgt
    15841 ccggctcaag caatatgacc aggcccgtga gacgtttcgc gggttagtgg cggatcgggt
    15901 gcaggaatcc tccgaggcca cggtatggtt ggcgcgggtc tatttgcgac agaatcaggg
    15961 cgacaagctt atcgccttgg cgcgatcggt cgcgcagggg tcccttggtg gcgatcagcg
    16021 ggcgatggtg cacctcttcg ccggagtgtg gttggaggat caggggcaat tcgatgaggc
    16081 gatcggcatg ttccggcagg tcgcgaagct gggggattcg gcgagtcagc gtgcggaagg
    16141 tctctggcgt gccgggtggg cgcaatatcg gacagcccgg tatcgggacg cagccgagac
    16201 gtttcgatcc gttgtggagt tgcatgtcaa cggctttgag cctcaggcaa tgtattgggc
    16261 ggcgagggcc gacgagcgcg aaaaaaatac caccgttgcc gatcaataca cacgcctctg
    16321 tcagcggcac gcctacagtt actactgcca gttggctgca cggcgagttt cgttgtcgcc
    16381 ggtcacgccg gttgcgacag acgcggagcg tccggcaggc gatgaggctt cacgattgcc
    16441 ggagaatcgg cgtcctgaga tcgaacggca cgtggtctat caacgtggaa tcgagctcaa
    16501 gatattgggg ttcgcccagg atgcggctcg cgaacttggc tctctcacag agcagtatag
    16561 tcgtgacccg gaggtgctgc tggccttctc gacgttgctc agtgaagtgg gggcgtacca
    16621 tccagcctta cgcgtggcaa aagttcactt taaggagaag ctggaacgca gcggtttgcc
    16681 gaccgcgccc gccttgtgga ccgtggccta cccgaccggc ctgctcccga cgatcaccgc
    16741 gcagggtgtc actgcggttg acccctacct ggctgccgcg atcattcgcg aggagagcca
    16801 gtacgatgag aaggctgtct cgatggtggg agccgtgggg ttgatgcagt tgatgccggt
    16861 cacggcgaat gccgtggcac agcggtacgg gtttcccgcg gtcggacggg aagaactgtt
    16921 cgaccaggaa accaacattc gactgggagt gcggtatttg gggcaattgc tcgagcaata
    16981 cagcgggaac ctggcccatg ccgtcgcggc ctacaacgcc ggcccgattg ccgtgaacaa
    17041 ttggatcgca gtgcatcgag gacgggagca ggacgagttc gtggaactca tcccgtatca
    17101 ggaaacgcgg ttgtacgtga aacgggtctt gcgcagctat ggagaatatc gccgtctcca
    17161 taacggcacg tcgtagcggg gccgttttct tgacaagccg gagcgaactt tctatatttc
    17221 gcccccgtca cccaacgtgt tcgaccacca ctttgatatg aggaaatacc atgacgttag
    17281 atcatctcga cgcccttgaa attcggattc gcgatttggt gaagctggtg caggatctca
    17341 agcggaaaaa tgcctcatta gaggatgact tgcgtctggc gcgggagcgg gttgcggttc
    17401 gtgatgacga aaaccgacgc tgggaacagg agcggatcga tatccgttca cggatcgaga
    17461 aagtcctggg cgagatcgat ctgttggaat gcctggatga acccaaggag gtggctgttg
    17521 actaagacca ttgacgtgga gatctatggc cagcggtaca gcatcaacgg ggaagcggac
    17581 gaatcgtacg tgaagcaatt ggccgatatg gtcgataagc agatgaaaca ggtggcggcc
    17641 ggcatgcggt cggccacccc ggccaagttg gccgtccttg ccgcattcaa ccttgcccac
    17701 gaattgatgg aatcagagcg aaagtcccgg cagggagagg ccgacgcgga tcgtcgggtg
    17761 gcctccctga tggaatcgat cgatcagcag atgccgtcca tcctgtcacg gtgagtttcg
    17821 ccttgctttc actgtactgc tttgttatcg tttctgtgtt gtattgacct gagggtcaac
    17881 aagagcagga gaggaaagga agcttcgaac aaggacggaa aatctgacga cggtttttga
    17941 gcgctaaaca tatggaatcg ctggtttttg tgctgttatt cacagtggtc gtgatggtgt
    18001 ggatgagaga aggctcccag gctggcatgt ctgttcgaca ggtgtgttcg caacgtgtag
    18061 caaggggcga agagcaggtg ctggcggatg ggctatggcc tgcgctgtgc tcgcaacctg
    18121 tgacgatccc cgaccggggg gcttggtctg tgcgccttcg atgtgacaga cgtattgatt
    18181 cgggtcgaag tcgaccactc ctccacgtga attgacggcg gcgattccac gcttcgtttc
    18241 tactggctca agaagtcaga tgtttctccc tcacgcgtag acattccttt ctcacgctcc
    18301 ttgctctccc tttaacactg gctgtgacta ttcgcagggt gacagtgttt actgtcccgc
    18361 tgccgtaagg gaggtggttc ccatttctct cagcgttgtt gcgtacattt ttgtcggatt
    18421 agtgggagcg gtcctgggtg ccgggttgta tgaggtcttt cgccgtcggt cggcgttggc
    18481 tcgtcgtgcc gaggccgagg atcagtcggc gcagatcgtt caatcggctc agcgtgaagc
    18541 agagaatctc gtcaaagagg ccaagctgga ggccaaggat ctggtctttc aggccaggat
    18601 cgaactcgaa aaggaacaaa aggccaagct ggccgaagtt tccaattcgg aacggcgtgt
    18661 gtcccaacgg gaagagggac tcgatcgcaa gctcggcttg cttgagaagc gggatcagga
    18721 agcgctcaag cgtgagcagg acttattgaa gcgtgaggag acactggtac agaaggacgc
    18781 ggcctgcgcg caggcgctga aacagcatcg cgaggccctt gagcgggtgg ccggcttgac
    18841 ggctgaggaa gcgaagcggc aactgattca ggatctggat agtcaggcgc ggttggaagc
    18901 ggccggcctg gccaagcgcc tgctcgaaga ggccaaggaa aatgccgacc gggaggcgcg
    18961 ggaaatcatc gccagctcga ttcaacgagt gacccgggat tatgtgaatg aagccactat
    19021 ctccgtggtg ccgattgcca acgatgccat gaagggccgg attatcggtc gagaggggcg
    19081 gaatatccgg gcgatcgagg cggcgacggg gatcgacctc attatcgatg aaacgccgga
    19141 ggcggttatt atttcagggt tcgatccgct tcgtcgtgag attgccaagg tctcgcttga
    19201 gcggctcatg cacgacggcc gaatccatcc gacgcgcatt gaagagatcg tcgaaaaggt
    19261 gaaggtcgat atcgagaagc tgatgatcga agaggctgaa aaggtgatct tcgaggttgg
    19321 cttgtctgat tttcatcccg agttggtcaa ggtgttggga cggctcaagt accgaaccag
    19381 ctatgggcag aataatctgt accatgcgcg ggaagcggcc tacatctgcg gcatcatggc
    19441 ctccgagttg aaactcgacg tgaaactcgc caagcgcggc gccttgttgc acgatatcgg
    19501 aaaggccgtc agtcacgaag aagaagggcc gcatgccatg ctgggtgcgg agatcgccaa
    19561 gaagtacggc gaaaacgcca aagtggtgaa tgccattgcg gcgcatcacg aacaggttga
    19621 gcccatttgt ccggagaccg tactggtggc ggcggcggaa gcgctgtcgg cggcgcggcc
    19681 gggggcacgc cgtgaagccc tggagtcgta tgtcaagcgg ttggagaagt tggagtcgct
    19741 ggcgaccgtt cacaagggtg tgcagaaggc ctatgcgatt caagcgggtc gtgaaattcg
    19801 tgtcattgtg aagcaggaag atttgacgga cccggaatgt ttccagttgt cccgggatct
    19861 ggcaaagaag attgagcagg agctgaccta tccggggcaa atcaaggtga cggtgatccg
    19921 ggagagccgg ttcgtcgatt ttgcgaaatg agcgaacggt tatgaaggtt ctgatgatcg
    19981 gcgatatcat gggcgagcca gggcgtcggt ccgtggctcg cctcctgccg aaactgattg
    20041 ccaaccattc catcgatgtt gtggtcggca acggcgagaa cgtggccggc ggattcggta
    20101 ttactccgga cctggtggac gatcttttcg atctcggggt gtcggtcatc accacgggga
    20161 atcatgcgtg ggacaagaag gaaatcctcg atgtgtttcc tcgtgaaccg cgtctcttgc
    20221 gtcctgcgaa ttatcccacc ggagtgccgg gccgaggcag ttatgtgttt accacgcccg
    20281 gcggcgaatc gttgggtgtc ctgcatttga tggggcgggc gttcatgccg acgatcgact
    20341 gtccgtttca ggtcgcgaag cgggaaatcg aacgtctcaa gacgcaggtc tccgcgatcg
    20401 tggtcgatat gcatgccgag gcgacgtctg aaaagatggc gatggggcat tacttggatg
    20461 ggctggtgac ggttgtggcg gggacgcaca cccatgtgca gacggccgat gagcaaatcc
    20521 ttccgaaagg cacggcgtac atcacggata tcggcatgac cggcccgctt cattcggtga
    20581 tcggaattaa aaaagaactg gcgatcgaaa agtttctcac cggaatgccg cggcgctttg
    20641 aagtggcctc gggtcccgtc gtcttctgcg cggtattgat ggaactggat gccacgctcg
    20701 gcaaggcttt gtcgatcgaa cgaatccgag tggtggattg atctgttcgc ctagccattg
    20761 tctcaggctc ggtaagctat gaccggccaa acccttccgc tccctctgct tctttccgta
    20821 tccgacgtga cgcgactgat ccgtgattcc ctggaagcgc aattccggga tatctggatc
    20881 gagggcgaaa ttaccaacct tcgcgcaccg tcttccggac acctgtattt cacattgaaa
    20941 gatgagcaga gccagcttcg cggggtgctc tttcgatccg gggcctcgcg gcttcggttc
    21001 accctgcagg agggcctggt gatcgtcgcg cgcgggcgca tctcggtgta tgagccgcgt
    21061 ggcgagtatc aactgatcgt cgactcgctg gaaccgaagg gcgtcggggc gtttcaactg
    21121 gcgttcgagc aactcaagga gcggttggcg cgagaaggat tgttcgacga ggcgcgaaag
    21181 cgtcccctgc ctcccttccc acgcacggtg ggcgtggtga cctctcgcac cggggcagcg
    21241 gtccgcgata ttgtggcggt cctccgtcgc cgctgtccgg tcgcgaatat tctcatcgct
    21301 tccgtgccgg tccagggcga gggagccgcc gagcagattg cggaggcgat cacgacgtta
    21361 agcggaatgc cccaggttga ggtgatgatc gtcgggcgtg gaggcggcgc ctcagaggat
    21421 ttgtgggcgt tcaacgaaga agtggtcgtt cgcgccattg tgcagtcgag ggtcccggtc
    21481 gtgtctgcgg tggggcatga aatcgatgtg acccttgccg actttgccgc ggactatcgg
    21541 gctccgacac cctcggctgc ggccgaggcg gtggtgccgg tgttggacga gatcgtggag
    21601 cgactaggcg agatgtctga tcgtctgtat cgagtcgtgc gcactttgtt ggagatgcag
    21661 cgccatcggt tcgagcgatc ggttggagtg atgcgcgaca tgcgctttcg agtgcaggcg
    21721 catgcccagc atctcgatga attgcgagac ggattgacgc gcacgctgac cgaacgatta
    21781 actctgctcc accgggggat ggtggtgcgg caacatgcct tgctggctca aggaccgcac
    21841 aaccggattc agaccgcgct ggtggtgatc ccgcaactgt ataaacggtt ggagcaagaa
    21901 gccagacggg gacttttgtc caggcggcaa gcggttgcgt cgtacatgat ggctttggac
    21961 gctctcagtc cattggcgat cttaagccgc gggtatagcg tcatccaggc aattccgtcc
    22021 gggcggatcg tccggctggc gtcagacgtg gcggtggggg atgtggtgca ggcgcggctg
    22081 gccgaggggc gtctgttgtg tctcgtcagt gaggtgctgc cgccttcgat gccttgacct
    22141 caccggtggg gcaggcaata atgttctgtt tgatgaatga gcaagggagt ggttgtggct
    22201 gccgtgaagt ttgaatatgc aatggcacga ttggaaacga ttgttgccga acttgaaaag
    22261 ggcgatcttc ctctcgatga ctcgctgaag attttcgagg aggggattcg gttgtccaag
    22321 acctgcctga agatgctgga ggatgccgag cggaaggttg aaatcctggt gcaagagaag
    22381 gacggcaaga agcggattca agcgttttct cccggtgagg atgagccgga gcgccctcag
    22441 taacggtctg atcgatgcac gggctttcac gttgcccgtg tctgtcgcaa ttaaacttcc
    22501 atcacagcct atgagccaga aattacgccc agagcgggaa cggctggatc gcgtgctggt
    22561 cagccgaggg ttggttgcca gtcgggaaga cgcggctcga ctcatcctgg ccggcctcgt
    22621 ccgtgtggat ggggtggtgg ttgataaggc ggcgaaaccg accttgtcgg atgcgaaggt
    22681 ggagctgacc gggccggggt ctccctatgt cggtcgcggg ggcgagaagt tagccggcgc
    22741 gctggatcaa tttcaggtcg atcccaaagg aatgatgtgc ttcgacgtcg ggtgttcgac
    22801 cgggggattt accgattgtc tgctgcagcg cggcgccgcg cgtgtctatg ccgtcgatgt
    22861 ggggtatggt cagtttgagt ggcgcctacg gcaggatccg cgcgtggtgc tcatggagcg
    22921 gaccaacatc cggtatcttg agccgagtgc gatccgggat ccgatcgatc tgattgtcat
    22981 cgatgtgtcg tttatctcgc tgaccctcgt gctgccctgt gttgtgccct acctcgctgc
    23041 gtcggggttc gtgatcacgt tgatcaaacc gcagtttgaa gtggggaaag gtcttgtcgg
    23101 acggggtggg attgttcgtg atgatggctt gcgggaaggg gcggccgaca aagtggtggc
    23161 gtgcgcgcag cagttggggc tggagttggc ggatcgaatg gaatcgccca tcgagggccg
    23221 aaagggcaat cgggaaatcc tggcctggtt ccggcgcagg gcgtgagggg tacgagtctg
    23281 gatatcagtg ggtctgcggc gggtttcgtc cgggacgttc acgcgcaagg gcaacacgaa
    23341 aacactttgc ggaagcctgc agaaggtgta ggctgaccct caattgttcg gtcaggggat
    23401 cggcacatcc gcagacgaag gagaggcgag gagacgtatg aaagtcttgg tcacgggggg
    23461 cgcagggttt atcgggtcac atgtcgtgga tcgtttgctc caggaggggc atgatgttgt
    23521 ggtggtcgat aatcttgtca cgggaaagcg gaaaaacgtc cccaaggcgg cccaattcta
    23581 caaactggat atagaaaatc ccaagctcga acggattttt cgcaacgaac ggccgtcgat
    23641 cgtgttccac ctcgccgctc agatgaacgt gcggcgttcg gtcgaagatc cgatgttcga
    23701 tgcgcaggtc aatgtgctgg ggacgctcaa tgtgctggag caagcgtcga aacatggcgc
    23761 gcgcaaggtc atcttctcct catccggcgg ggcgatttat ggcgagcagc tggcatttcc
    23821 tgccccggaa actcacatta cccagccact gtcgccctac ggcatcagca agttatgcgg
    23881 ggagcattac ctatcctact accaccggtt gagcggtatt caggtggtca gtctgcgata
    23941 tgccaatgtg tacggcccgc ggcaggatcc ggaaggggag gcgggagtcg tcgccatctt
    24001 cattcaaaag atgctgcgtg gcgaacaggc ggtggtcaac ggaaacgggc gtcagactcg
    24061 cgattttgtg tttgtcgagg acgtggtgga atcgaatttg atggcgatgg gtcccgaggt
    24121 ggagggcgtg tacaacgtgg gcacgggaat cgagacgtcc gtgaacgacc tcttcaaaat
    24181 cgtcgtcgat ctaaccaagg tagagtttaa ggaagtgcac ggcccggcca agcgaggcga
    24241 gcaagccaga agcgtcatcg actccacaaa gctccaccgt gaccttgggt gggaaccgaa
    24301 ggtcgatctg cgcgagggac tgcggcggac ggtcgaatat ttccgcgacg ggctcggata
    24361 gccgcttgag ctaatagcgg ggcacggatg ggtctacctg agtagaccag gcatcaatgc
    24421 ctccgatcag gtttttcacc tttccgaagc cttgctgcag cagaaaccct gtcgcatccg
    24481 cacttctcat cccgtggtgg cacacggcga caatctcggc gtcacggtca agtttgccaa
    24541 gagattgctg cagggtcgcc agcgggacca ggatggatcc ctccaacttg gcgatggcat
    24601 attcccatgg ctcccggacg tctaccagca ccagcttgtc gcctttatcc agcctcgact
    24661 tgagatcctt aggggtaatc gtgaaactca tcagcgcacc tccatctata cggtcggatc
    24721 ataaggagcc gaaaaatggg tgtcaactcc ggtgtggtcg gccattcgtc gcgtggggtc
    24781 tgatctcaag actttgcgga ttcagaccga agcgtatgga tgagaggacg agggcctggg
    24841 cctgacagtc ggtcggcagg taacgcctct ggagaatggt cgttttttgt cgaattgtgc
    24901 aaatcctgcc tagtttttta agcgtaatcg gcacggagat ggggtagaat tctgccctaa
    24961 gtgcttgata actgaagcgc aaacagtact cgccgttctg tgtcggcacg caatttgctt
    25021 agagctgtgg gggagacgtg ggagcgaacg gttcagaggt agtggtgttt gtgactgcgg
    25081 caacggccga ggaggcagaa agactcggtc ggatcatcgt ggagtccaga cttgccgcct
    25141 gcgcgaatgt cttaaatggc attcgatcga ttttccgttg ggagaataag atcaacgtcg
    25201 aaaatgaatg cctgatgctc attaaaacaa ccctggagca atacccggaa ctcgaagcag
    25261 tcattcgacg gcaccatagc tatactatcc ctgagatcat cgcactcccg gtcattgcgg
    25321 gatcggcgcc ctacctggaa tgggttcggg aagagactcg taagtagttg attctaataa
    25381 tgtaattttt gaaaacaaag ggttgaccat cgtgctagag atcagtacac tgcaaaagtc
    25441 agtggctgaa ttcttggttg aatccgtgaa ggggagagcg tcctatgagc caacaagcgg
    25501 ccgaaacaag tgatgcctat actgtggtgg tctttcgggg gtcctcctcc aagccgttac
    25561 ggtttagttt tccacggaaa ttcgtgcgca agctcctgat cttggctgcg atcctggtcg
    25621 tggcagatct gctcgttatc tctcattatg tgattcggac cggagaagtc tggcaattgt
    25681 cggcgttccg ggctgaagcc atgggtgcgc gtgagcaaac cgctgccttc tccgctgcga
    25741 ttgatgatct gaaaaagcgt ctctcgacca tgggcgaggt caatcaacgg cttcgagtca
    25801 tgctgggaat tgacgccagc aagccggccg gggatttggc gaatggacgg ggtggcgagg
    25861 acggtccgct gccggatgga aaaaccagtg tgcaggggaa tggatcagcc acttctgggc
    25921 tggaaccgcg gcagcaggtc tccgaagcgc gtgatgccaa tcagtccgag gttgattttg
    25981 ctacggaaag tattgaagag gtcactcagc aggttcgcga aagccttgag gccctcgtcc
    26041 gggaggcgaa gcaacaagag gaagccctcg aaagcctgac ccaagttgca gagcagcgtt
    26101 cgactcagtg ggcgtcaact ccctccatct ggcctgtgcg cggatgggtg acctcggcgt
    26161 ttggcccccg ggtttcgccg ttcaccgaaa agccggcctg gcatgacggt ctggatatcg
    26221 gtgctcaggc aaattccccc gtccaggctc cggcgctcgg gcgtgtcgtc accgtggcct
    26281 ttgactcgaa aatgggcaat atggttaagc tggaccatgg atatgggatt gagacggtct
    26341 atggacacct tgccaagtcg ttggtgaaag aagggcagcg agtgaagcgc ggtgatgtgg
    26401 tcgcgcttgt tggcagcacc gggctttcca cgggaccgca tctccattac atggtcaaga
    26461 agaacggcca agcgttggat ccgaccaagt ttattctcga ctagtctttt cgcattcctt
    26521 ccagatttcg aaagaagttt acccgttccc ttcctctttt ccgttgtaat cctcgactac
    26581 ccgcgtccca gtccagctct accagccctg tgctccccag tctttgcggc ccattacttc
    26641 ggcgaatgcc gtggtatact ccgcactttc ctgaaattcc tgttctcacg aggaacattg
    26701 catgactgat ctggagatag cccgttccgt ccctcacaag catatattcg aagtcgcaca
    26761 gtccttaggc attcattctg acgatctcat tccctttggg cgctacaagg ccaagatcgc
    26821 gcccacgctg ggtgagcgaa tccagaacag accgctgggg cgatacatcc tggttacggc
    26881 catcaatcca acaccccttg gcgagggcaa gactacgacc tcaattgggc tgagcatggg
    26941 gttgtgccgc ttaggccatc gggccgccgt cacgctcagg cagccttcct tgggaccggt
    27001 cttcggaatc aaggggggcg gtaccggagg cgggagagcc caggtccacc cgatggaaga
    27061 tatcaatctg cattttaccg gcgatgcgca tgccgtttct gcgagccaca atctcctttc
    27121 cgcatttgtc gataaccatc tgttccacgg aaatacgctc aaggtcgatc cgaaagggat
    27181 cagatggcca cgcacgcttg gggtgagtga ccgcgcgctt cgcgacattc tcctgggaga
    27241 ggaaaccggg cggcggccgg ggaagtttgt gattacggag gcgtcggaag tcatggcggt
    27301 gctggccctg gcgaccgacc atgcagatct tcgccaacgc ctcgggagaa ttctgatcgg
    27361 cctgactgag ggcggacaga tggtccgtgc tgaggaattt ggctgcgccg gctccatggc
    27421 cgtcttgctg aaggatgcct tgttgccgaa tctcgtgcag accctggagg gaactccggc
    27481 atttgttcat gccgggccgt tcggtaacat cgcgcatggg aattgctcga ttgtctccga
    27541 tcggttggcg ttacgttgtg cagactatgt cgtgactgaa gctggattcg ggagtgattt
    27601 aggcgcggaa aagttcttca atattaagtg ccgcacgtcc ggtcttcgtc cggatgtcgg
    27661 ggtggtggtg gcgaccttgc gtgcgctcaa gcttcatggc ggtgggggaa tcgtgaaatc
    27721 cggtgctccg ctgcctcccg gtgtgaccgg gccgaatcag gcggcgctgg agcagggctt
    27781 tgccaacctc gagcagcata tcgccaatgt ccgggcccat ggagtgccgg tggtggtcgc
    27841 ggtgaatgcg ttcaaggatg attcaaccga tgaactaaac tgggtgtgcg aacgcgccct
    27901 ggaagggggt gcgtcggcag ctgcggtgtc gacccattgg tccgacggtg gacggggagc
    27961 cgaggagctg gccagggcgg tcgtcaaggt cgctgcgcaa ggtacgcaat tcagtcctct
    28021 ctatgaggtg acctcgccca ttaagaaaaa gattgagacc atcgccacca cgatctatgg
    28081 ggcggccggc gtgtcattct cgcctcaggc cgagcgggac gtagagctgg cgacacgatt
    28141 gggattcgat cgacttccca tttgtatggc gaagaccccg ctttcactat cgcacgaccc
    28201 ggcggtgaag gggcggccct ccgggttcac ggtgccgatt caggagttgc ggattctggc
    28261 cggggccggg tttctcaccg cggtctgctc aggcattcag ttgatgccgg gtctgccgaa
    28321 gaagcctgcc ggggaacgga tcgatgttga tccacaatcg ggtcaaattg ttggcctgca
    28381 gtagtggcgt gttaccgctg tttcaggtac cggaagaatt ctgattcggg actgacgagg
    28441 atggtggtct tgtccttgag ggtcttgcga taggcttcca tggtgcgggt aaactcgaag
    28501 aaatgcggat cctggcgata ggcatcggca tagatgcgga acgctttggc gtctccgcct
    28561 ccgcgcaatt cctctgattc tcgataggcc tctgcgagga tgatctcccg atctttctcc
    28621 gcctccgact tgattttctg agcctcttcc gcgccttctg cgcgatactg cttcgcttgt
    28681 cgctcccgct cggcttgcat gcgcgagaac acggcctttt cattctgctc aggcaggtcg
    28741 gctcgtttaa tccgcacgtc ctggatttct atgccatagg cagacgcttt ttcgttggcg
    28801 cgttgggtga ccacggccat gagttgcgca cgggccgatg agacgatttc cgccagatcg
    28861 tgccggccta attccacccg cagttccgaa taaataatat cgtgcaggcg ctggagcgcg
    28921 ccgcgctggc tttggaacgc ctgatacacc ttcagcgggt cggtgatccg ccacttcgca
    28981 aaattatcga gcagcaaggt cttcttgtcc tgggtaatga cgtcctgcgc attggagtcg
    29041 tagtccagaa ggcgcttgtc gaagtaggtg acttcttcga tgaacggcat tttgaggtag
    29101 agaccccctt cggtgacgtt ccggaccggt ttcccgagct ggactacgat ggcattctgg
    29161 gtgacatcta cgatgtaaaa aggcgatgca ccgaggacga gcagcccgag aacaatgccg
    29221 acaaatgcca ggataaatcc ttgcttgctc atggcgtgcg ctcctgtgtc gaactggacg
    29281 gggccggttt ggagaaccga tcgagcggca gatacggcag cgcacggtcc gcccctttgc
    29341 cgtccaggac aaatttatcg atgtggggga gcacgtcttc aagggtctcg atatagatcc
    29401 gcttgctgat aatgtctttc gcctggttgt attctttcag cgtcgcgagg aatcgattgg
    29461 attcgccctg ggagcggttg agccgcgcct gcgcataccc tttggcctgg ttcaccaact
    29521 gggccgcttc acccttggct ttgggggtaa tatcgttgcg atagccctga gcttggttga
    29581 tcagcttttc acggtcttcc ttggcgttgg tgacgtcctt gaacgcggcg gccacggctt
    29641 cgggcgggtc gacatcctgc aactggaccg cggcaacctg cacgccggtc ctatagtcgt
    29701 cgaggatgtg ttggagaagt tcctgcgtgt cgttctggat ttgtgctttg cccgtagtga
    29761 gagcttcatc gattttgctc ttgccgatca cttcccgcat agaggcttca gccgccttcc
    29821 cgatggtctc gtcgatatcg gccacattga agaggtactc gcgcgaactt ttgattttat
    29881 attggacgat aaattcaatt gccagaatgt tcatgtcgcc ggtcagcatc aaggcttctt
    29941 gcggcaccat ctgctggcgg ccttggcgat cctttcgaaa tccgatctcc acccggtgaa
    30001 gcttcgcgac cttcggttgc aagacgcttt cgatgatggg aatcttcatg tgtgggcccg
    30061 gatccacaac gcgcacgggg ataccgaagc gtttcaccac gccttcttcg tccggtgcca
    30121 caataaaggc gctttgccag atgaggaaga ccgtgaaggc gacaagaaga agattgcgga
    30181 aaccgcccgt aggtaacaga ttgcgaagtt ggccctgggc ctgcttgaag gcctgatcca
    30241 aatcgtcgcc cttcttgctc caagggtctt tggggtccca gaccatgtag tggctcaatt
    30301 cagtgagggt ggcaatgtcg ccagcgaatc accttagcaa agtgaggacc gtcgagcaac
    30361 acataaaggc ggccggttga cttatcaaga ttcgttgata tatatttttg tcatgaaggg
    30421 aagcacggtg cgaaatccag cgcgggcggc ggagttgttt cacgcgcttg cggacccgac
    30481 gcgattggag attctggacg agctgaaaga gggcgagtgc tgtgtgtgcg aactgacgga
    30541 tcgcctgcag gccgggcagt cgcggctctc ctttcatctg aaggtactta aggacgccgg
    30601 aatgatcttg gatcgacgag agggccgatg gatgtactac tccgtgaacg ccgacgccct
    30661 tgccgagctc gatgacctcg tcgattcgct caagcaggcg aagcgggcgc gccgcgccac
    30721 cggctgttgt tgattttttt ccgcctaata gatcaatgaa tcttgatgca taaaagagga
    30781 ggcgacgaaa tggacgcaca agcgattcga gagctggtca aggagaaata cggtgaagcg
    30841 gccgctagag ccaagagcgg aggcagttcc tgctgcgggg cctcccccgc gctgatgaat
    30901 gtcgatccga tcacgtccaa cctgtattca gatcaggagc gtcaggggtt gccggcggat
    30961 gccgtggcgg cctcgctggg ctgcgggaat ccaacggctc tggcagaact tcatgccggt
    31021 gagacggtgt tggatctggg atccggcggc ggcatcgatg tgttgctctc tgccaagcga
    31081 gtcggtccgg cggggaaagc ctatgggttg gatatgacgg agcagatgtt ggcgttggcc
    31141 agagaaaatc agcgcgcagc cggcgtggag aacgtcgagt ttctgaaggg cgaaatcgag
    31201 cacatcccgc tgcctgatcg ttccgtggat gtgatcatca gcaattgcgt cgtgaatttg
    31261 tccggcgaaa aggagcgcgt gctggcggaa gcctttcgcg tcctgcgtcc gggaggccgg
    31321 ctggcgttgt ccgacattgt agtgcgaggg gcgattccct cagagatccg acggaatctc
    31381 gaattgtggg ctggttgtgt cgcgggcgcg ttggaagaga cggagtatcg ggacatgctg
    31441 gcccaggcgg ggttcgtcga gatcggaatc gagccgacgc gcatctatca ggccgatgac
    31501 gtgaaggaat tgctggtcgg cacagacctc tccagtgatc tgctcgtggc ccaagtcgag
    31561 ggcaagttca tgagcgcatt cattcgggcc aagaaaccgg ccgtgacggt ctaactccgc
    31621 cgggcagcgc gagcacgagg gagcaggagc gatggaactc gcattatcgg tcgatgagcc
    31681 gcaggtggct acgaagcggc tgtcgttctt tgagcggtat ctcacgctct gggtggcgct
    31741 ctgcatggtg gccggtgtgc tgatcgggca ggcggtgccc ggtttggtgt cgagcctgcg
    31801 aggggctgag atcggacagg gcagccacat caatctgccg attgccgtgc tgatctggct
    31861 gatgattgtg ccgatgatga tgaaggttga gtttgcctcg gttcgtgacg tgggcaagcg
    31921 gccggtcggc ctgctgatta ccctgtttgt gaattgggtg gtgaagccct tctccatggc
    31981 gttctttgcc tgggtgtttt tccggtacgt cttctcagcc tggatctcac ccgccgaggc
    32041 cgaccaatac atcgcgggca ccattatcct ggctgcggcg ccctgcacgg caatggtgtt
    32101 tgtgtggagt tacctgacgg acggagaccc cgcgtacacg ctcgttcagg tggccgtgaa
    32161 cgacctgatc atgctcgtgc tgtttgttcc gattgtcggg ttgctggtga gcggcgcgtc
    32221 atcattggcc gtgccgttcg acgtgctgct ctattcggtg gcgatcttta tcgtcattcc
    32281 gttgatcgtc ggcgccgggc ttcgactgtg gctggttcgt cggtacgggc tgcgttggat
    32341 ggaagaacag tttctgcccc gattcgcgcc ggtgacgatc gcggcgctgc tcctcacgtt
    32401 ggtgtgtatc ttcgcgtttc agtcgcaaaa catcctgggg aaaaccgtac atgtcgtcct
    32461 gattgcagtg ccgattctcc tgcaggtcta catgaattcg gcgctcgcct atggcctgat
    32521 gcatcgctgg cgggttccct acgcgatcgc cgcgccggga gcgctcatcg gtgccagcaa
    32581 ttttttcgaa ttggctgtgg cgaccgccat cgccctgttc ggaccggagt ccggtgccgc
    32641 gttggcgacg gtggtcgggg tgttggtgga agtgccggtc atgttgtccg tctgcagcct
    32701 gtgcaacaag accaggcatt ggttcaatca ggaggtcggc acatgaaagc acgagtgcta
    32761 tttctctgta ccggcaactc cgcgcgcagt cagatggcgg aggggtggtt gcggcatctg
    32821 gcgggcgatc ggtttgaggt gttcagcgcg ggcactcacc cggttggctt gaactctggc
    32881 tcggtcgaag cgatggccga ggtgggaatc gacattgcgg gacatcggtc aaaacatgtc
    32941 tctgaatttc tgacgcagcc gttcgattac gtcattacgg tctgcgaccg ggccaaagag
    33001 tcctgcccga cctggccggg gcccacccat ctgttgcatt ggagttttga cgatccggcg
    33061 gcagttaccg actctgacga agcccatcga cagctgtttc gacgagttcg cgacgagatt
    33121 cttggacaga tcaagggttt cctggctcag gaggcaaagt cacttgtctc aggtccttgc
    33181 taggtgacgg cgccgccgca ggaggaaccg gagccggctg tgcacccgta gcagtggttc
    33241 ctcgtcacga tctgccgatg gtgcaggcgt gcgggatcga agtctcgaat gtgttgtggg
    33301 gttccctgcg agaccggcaa ttccagcatc tgattgaagt cgcaatcgta gagggtcccg
    33361 tcccatccga ccgacagggt gtagcggcac atgacgcccg ctgccgccgc cgggttgaag
    33421 gccgcggcaa gccgttccat gtagccttcg tagttgccgc tttcgatcag gaactccaga
    33481 aatcggctga tgggcatatt ggtgatggtg tacaggcgcg tgaagctgac gccgtgacgg
    33541 gaggccagct ctttccggaa cttcgcctcg atcccttcct gcttcggcgg aagaaatgcg
    33601 cccaccgggt tatacaccag gttgagcgtc agtccgctgc cctctttgcc gtagcccagt
    33661 cggttcaata accgaagggc ctccatcgat ttgtcgaaaa taccctcgcc gcgttgcgca
    33721 tccgtctgtg tcgcctgata ggacggcagc gatgcaatga cctccacgcg gtgctgtgcg
    33781 agaaactctc ccaggtcggc ttgcgaaggc aggagcagca cggaaagatt gcagcgatcc
    33841 agcacctgac ggcccaataa tcgggcctgt tcgacgaccc agcgaaagtg cggattcagt
    33901 tcgggtgcgc cgccggtgat atccacggtg ggaatatccg tgcgagctag ggcctggaga
    33961 cagaggtcga gggtctcttt cgacatgacc tccgtccggt ccggtccggc gtctacgtga
    34021 cagtggcgac aggtttggtt acacagcttc ccgaggttaa gttggagcgt cgtgatgccg
    34081 gtcgcgtgaa gcggatagag ctctaccgca ttgagggccg tctcgaacgg ctgacaggcc
    34141 gtcgtctggg ccaggagtcg cagctgctcc gatgcggctg caagcggatc tcgtcgagcg
    34201 agcaggctta gacccatcgg atcgatccct tcctcatcgt caggggttca gcaacaaccg
    34261 ccggtcgggc tacaggcgac ttccgtgccg tccacacctg ccgaggctcg ttggtagatc
    34321 gtgaagtgag ggcgataggc ctcctgtgtc aggacctgga gggtcttgga acaaatttcc
    34381 aacggcacgc cgcggtagta ctggtgatga tcgtcgtcct cggcactgat gatcggcccg
    34441 gtgagaatcg cataggcgcc ttgccagaca cagggtttct gttccggatg gattgcgcac
    34501 cagcgtggat cggtggcagc gagtgtgacg ggcgtagggc caagaaggaa cagcgaacga
    34561 agcggctcgc tctgcaacag ttgcgcgcgt tgggcgtcga tgctttgggg tacgccgcgt
    34621 tcgtaccgtt ggcccaattc gtccgtgacg ctcgagaagg ggccccggag cgtggcatag
    34681 gacgaggtct tttcgtcggc gatggctgga aacttgtatc cggtgagcgt caccgagagg
    34741 aaatggatgc cgtcgatcga ctgccagggc gtggacttga tctggtggac ggcgcgaaat
    34801 cccgcatcga ccatcccgcc gatgtagtcc tgcacctgca aggcgccgga cagacagtcg
    34861 ccccacttgg ctgcgtcatg caccaggtat tgcggcacaa cctggtccga cacgatgtcg
    34921 gaaatcgtaa accggccgcc cggcttgatc acgcggtaca tctcgcggaa gacctttcgc
    34981 ttgtcgggcg ctagattgat cacacaattg gaaataatca gatcgacgct ggcgtcctcc
    35041 accggcatgg catcggccat ccccttgcgg aactcgatat tcgatgtcgc gtagcccagg
    35101 tttccggcca cgatgggcgc gttgcggcgg gcaatctcca gcatggtgtc cgtcatgtcg
    35161 atgccgatga cccggccggt gggtcccacc cggcgcgccg cttcaaaaca atcgatcccg
    35221 ccgccggagc cgatatccag caccgtttca cccgattgaa cggtgttcag cccggccgga
    35281 gtgccgcagc cgtaggagat ggtcaggact tcttccggaa caaacgattt gaggtcggca
    35341 aagtcatacc cggtcgggca gcacatctgt tcgccggttg cggcggcccg ggcatagcgg
    35401 tcactgactt tctgcgtgat atcgtcgatg gccataaggt cctcggagtg ggataggaaa
    35461 tgccgtcgaa tgatctatca agagaagagg cggcaggtca acaaccagag gcgggggcag
    35521 ccggttcggc tgcccccgca ggtcacgagt gtcgtacacg ttcgggaact actgcatcga
    35581 ggccatcggc ttctcgacaa tgtcgccctt catcggaccc aatgcgccca gcagctcgtt
    35641 cttttcccgt tcaggcactt tgaatttgtt caacgtggcg acgaggtctc cgaccagcgc
    35701 gtcgaagtcg ccgctgctga cccccatgcc ggcatgggtg ctcttcatat cccgcccggt
    35761 ataggtgcaa ggtccgcccg acgcctgaca gatttgatcg accagcatcg atttcaggcg
    35821 cgggatattg gcgttggcga acttcccgtt gatgcgggtg tcggcggcca cgcgcccgac
    35881 gaagtcgtcc accactgcgg taatggccgt tttgccaccc agccggtcgt agagagattt
    35941 ttcgcctgct gtggtaccgg ccggtcctga accgaccgta ttgcaggccg cgacggtcag
    36001 cgcgaggcca aggatcaaag aaagggatgc cattcgttgc gacatgagag tacctcctgg
    36061 ttatgggtct gacggttggg ccgggtgtgt ctcgacatcg accggctctc tgtctcgaac
    36121 tacgaggtgg gatggcagtt ggatttccgg ggaacgaaaa aaactgcgtt gtgccccgat
    36181 cgttccgatg ctacagtttc ggcggatcgg cgcaggcaga gggaatctaa ttcaccgggc
    36241 agactcgtag tacttgacga ggatgctcat gattcattca caggctgtgg cacagacacc
    36301 tttgatgtat gagcaggaat gggcgggact tctcgcgcgc attgccgccg gagaccagcc
    36361 tgcgcttgcg gagttctatg atgcgagcag tgccaaggtg ttcgggttgg tgatgaagat
    36421 cctcgcggat cgtacggttg ctgaagaagt caccatggat gtgtatacgc aggtgtggcg
    36481 ccgcgcctcc agctacgacg cggagagagg cacgccgggg agctggctga tgacgctcgc
    36541 gaagacccgg gccatcgacc ggttccggag cagttatctg gagcgggggc ggcaagtccc
    36601 gctcgatcac gcggcggagg tgccgggtga cagggccacg cccgagcaat acagtgcggg
    36661 gctcgaacgg cagcggctgg tgcaggaggc catggcgagt ctctccgccg agcagcgtca
    36721 ggcgattgcc ttggcgtact actggggttt gagtcagagt gaaatcgccg accggttgca
    36781 gctgccgttg gggacggtga agacgcggat gaggctgggc atgatcagac tgcgcgaggt
    36841 cctggctccg cacggggaag gggtgcggtc atgaccgatc cacgccacaa ggaagacatg
    36901 tcggagcatg cggcactcta cgccttggaa gctctgggtg atgacgagca gcggcaattc
    36961 aagcaggccc ttgagacggc tcctccggaa gtctggcagc aggtcgcagc gtttcaggag
    37021 gtggtgcaag agttggcatt cagcggtccc gccatcgcgc ctccggcgtc actcaaggcc
    37081 aagctcatgg cgcgcatcgc gcaggaaccg caggagcgac ccggcggagg aacgttcacg
    37141 tttgttcgat cggggcaggg ggcgtggcga gacctgggtt ctggtgtgtc tatgaaactg
    37201 ctgttcaacg attcggccgg agcacgggcc acgatgctgc tgcggctggc tccgggtgga
    37261 aaactgctgg cgcaccgcca ccggcaggtc gaagagttct atgtgctgga agggagctgt
    37321 atctgcgccg gggaattcct ccaggcgggg gactaccatc gcgccgaggc cgggagcgtt
    37381 catcccatca catcgagcga gcagggttgc ctggccctgg tgatgacctc gacacagaac
    37441 gaaccagccg ggtaatcaat tcactcctgt tgctgtcctt ttcgcggcct tccgaagacc
    37501 cgccaataga tcgttacgtt caccagcacc accgctgtcc ccagcagaat ctgaatgttg
    37561 ctggtcagcc ctgctggata catcagcggc aggaggtagt ggccaatgaa gtcggagcca
    37621 taggctgact ctccggccat ggcccgcaga gtgtgttcaa tcggggtgag cgggcagatc
    37681 caaccggtgt actcgacaat cgccccccag atcgctgccg gtagatgcag ccaggcaagg
    37741 cgaggccatt tcaatagcaa cagcccaccc gtcacgacga agagcacaaa ggccaggtgt
    37801 agcagcagca cgagatccgc gccgattcgg taccacatgt gcggcagtct gaagaaacgg
    37861 cgcgatgaag gcaatcaaga gacggcgagc ggagccggtt gccgcgtccc tacatctgag
    37921 cgggaaggtc atagccctgc ggcggggcgg tggtgaaggg aaggggcaac tgcgcggtgg
    37981 gtaatgtcac ggcgctgcga gcccaggcgg ccagcgcctg cgggtcctcc agaatatcca
    38041 ccggcacttc gtaatactgt cggagcgttt gtttggcgcc cggacggaag ggatgcatgc
    38101 cgcgcgcgcg atagagcgct tgcgtcacgt cgttagtttt gaagtagagg cgtcccttgt
    38161 ggatgatgcc gaagaaggtg gcccgctggt agaggccata cccgccgaac atggctttcc
    38221 ctgtgagctc aggcatctca ccgagttgat ccaggacgaa gtctttgaag ctgtcgtggc
    38281 gcatgcgtcg agactgattg ggtggaccgc cgctcatgtc ggccgtatcg attcggcgca
    38341 ctctgcaccg atgactcaat tatttcaact gccgggtggc gaggggttcc gttgtgtgtc
    38401 cagggggtgg tcgtcattcg aggcacaagc gtgaggggga ggctttatag tatacggagc
    38461 cgtgcgagtt gatgaacgtg tggaaggtgg aggctgaaac caggatgtgc ggtctctgtg
    38521 ctgcagcagt cctcgtcgcg cgtgaacccg gtcgccccgt ggatgcgcta ttgcatgatt
    38581 tgagcacatc ccacgaggcg caagcgattc aggggtctca gcggcaggag gctaggacac
    38641 cttgatctcg atcgatttcg gttttgcttt ctcggatttc gggagatgca tgtggagcac
    38701 cccgtctttg tagtcggctg tgaccttgct cccgtccgca tcctccggga gtgagaagct
    38761 gcggacgaaa ctcccgtagg cgcgttcgat ccggtgatgc ttctcccttt tctcttcctg
    38821 ctcgtatttc cgttcgccgg aaatcgtcag caccccgttg tccacggtga gtcgcacgtc
    38881 ttcttttttc atggccggca actccgcctt gatgcggtat tcattctcat cttccacgat
    38941 gtccaccaac ggcgaccact cggcaaccgt cagggcttcc ttgcccccat tcccggtcga
    39001 tgcccgatgt cccaggaggg tcgacaatcg gtgttccagt tcgtcccgtt ctttcaaagg
    39061 attccactga actcgaaacg gatcccaacg tatgagtgtg ctcatggctt catctcctcg
    39121 tatggtcgaa atggcatcat gacgactcga tgtcggtgcg attagtggcc cgctgcggca
    39181 gccagctgac gttcggcgtt taaccagtcc tccagagcct gcccgtcttt ccgccctcgt
    39241 ttctcataca gttcgtaggc gcgtagggcg atcgcagcgg ttccctttgc ggcctgcccc
    39301 gcgtgcgcgg ctttgtcatg gctcgggctc gtgtgggctg tgccggcccg ctgcggggtg
    39361 gttcgactga cattcgtttc cattatggtg tacctccttg ttgagccggc tgtgtagcgg
    39421 ctgaaaccat cagaggctct gccgtccgcc gctacccttc tgtcggatct cggcggacga
    39481 agagcgggtc tgtgtgatga agaaaagggt taggcctttt ccttcaccgt gagatcgttt
    39541 ttcacagcct tcacgccggg cacttgccag gccgaccaag aggcttcggc gctggtcgcc
    39601 aggtcctgaa cttcgccgct gagcgagacg acgcctgcgt tcgtctgagc atgaatgccg
    39661 gctttcttta ggtgtgcgtc cttctccata tgctcgtgga cgcgggcggt gatggcgtca
    39721 tccttgtcgt ccgtcgcctc acgtttggag ggggccacga cctgcagatc gttcttcacg
    39781 gttttgaccc cgtcgatccc tttggcaatc ccttctgcag cctgcttggc cgcatccgaa
    39841 tcgaccttgc cgcggatcat gactgcgccc tgggcggtct ccacgttgat ctcgcttccc
    39901 ttcacccggg aatcggcaaa cagcgcgatc ttggtcttcg ccgtaatcca ggagtcattg
    39961 atgggtgtct tctcgtcggc cttgccgatg gcatgcgccg atccggtgat catcgccccg
    40021 ccaagaacca atgctgcgca gagggtcagt acattgcgtg tggtcatcaa gtctccttaa
    40081 gcgaacagtg aaagtgtgcg gcgaaatctg tttcgccgat ggtgtcgatt cagggagcct
    40141 tcgttccctc ggcgtccggc ttcgcctgct catgccagtg ggaagcccac ttcatcagct
    40201 cggccttctt ctcgccgtac cgttcctgca cttttccgac aaaggtgtcg taattgccgg
    40261 cgatctgctg caagtcgtct tcggtaaact tgccccattg ttgtttcaga tcccctttga
    40321 actggagcca ttttcctttg agttgatctg cgttcatacc gcctcctttc gtggtgtcag
    40381 ccatgaaacc tcggctgggt gtgactgtcg ggtccttata cggtgaacgg ttcagacagc
    40441 ctgaaccgtt gtcaggtggt tcgcggagtc cgtcccgaca ccaggtgcac caccagcaac
    40501 accaccccga tcaagaacaa gatccaagac atctggacgg caactgcgga gacccccaaa
    40561 agattgaggg cgttggcaat caagccgact actaagaaca tcagcgcgta gtagagcatt
    40621 gggcactcct ttcatggagt caccggcgtg agttgaacaa tcctccgggg cctcgaggaa
    40681 tgacggttgc gctccacgag cggaccggca catcggacag gcggcacctc agagagactc
    40741 tgggacagga gcggcggaga ctggggaatc agtcacgcgt acgaaggaac gggaagcgcg
    40801 aggatgggac agtcagcgga gcttcatcgg gcacctcttc acgctaagcg caatcggcag
    40861 tgacgtcaag cactataagg tgaggccgga acgcggaggc tgaagctaag tggaaacgcg
    40921 agtcgcaaga attggggaga tgtctcgatg tacggctagg ggttttgcta gtgctgcccg
    40981 ctcggtgatg gcggtacaag ggctataggc aactcaccac acgtggagga cgggcatgcg
    41041 tacattcatc attgcggcga tcgcggggtt ctgtctgtta acgggattgt cgttctctgc
    41101 cggagcgggg gaaaccaagg caaagatcga aaagggcaag ggggagctga agggcgatct
    41161 cgaagaaatg aagggtgaaa ccaaggcgat gaaggaagag ttgaagggga acgacacgaa
    41221 agcggagatg gaacgggcga agggcaaatt gaaaggcgcg ggagagcggg ccaaagggaa
    41281 gatgaaagag ttgaaggaaa aggcaacgga ctagcccagg ttcgtttccc gcccgccggc
    41341 tcgtcagggc cggcgggcac ggtattgtgc gagtgctgcc gtgcgacggc tactgcggct
    41401 tgcgcgcgct cacgatcctc accgcaatcg gcaacgagac tccctgtggc ccccgatact
    41461 cgttcacggc cttgacgatg ccccgctcgg cttcggcctg ttgctcctgc gtcaatttgg
    41521 caaacaggtt ttgaatcggt gcggcgatat ccatcaggcc gcgaaagaat tcctccgctg
    41581 tggcataggt cacctccgcc gtgaattctt catcgtcgag cggcttgagg cccgcccgtt
    41641 cgaacatgcc ggccaggtcg cccggctttg caagccggaa gatgccgggc gcctcagggt
    41701 cgggcggcgg caggggcgtg atggtcttga tcacgtccat tgggatacga atgaacggat
    41761 tcttgtccgg cgccgaccag acggcggccg cgacatagcc tcccggtttc aacacccggg
    41821 cgatttctgc ggcagccttc ggaatttcag gcaggaacat caagcagaac cggctgatca
    41881 cggcatcgat cgagccggtc tcgaacggca gtgaagtgac atccccggta cgaaacgtca
    41941 cgtgctccag gccgagcgtc ttggctttgc gcgtggcgac cgccagcatg gattcagcga
    42001 gatcgatccc gaccacggta ccatccgatc ccaccacttc accggccagc agcgccggat
    42061 agccggtgcc cgatccaaga tccaacaccc gcagcccggg ccgcacccgc gcatcggcca
    42121 ccagccggtg gttgatgaag gccatgttgc ggttgaagaa ctgatcccat ttgtcccaac
    42181 cggcggcaac gcgattccag tcctgacgct gggcttcgat cacagcctct ggtgtttgtg
    42241 acggcatcgg agcgactcct tgttggacaa agtagagagg aagattattt cacgcgttcg
    42301 aaggtaaaca ccttcggcac gtcttttccc gccttgctca aggtccaggt ctgggtgaag
    42361 tgatcgtggt ctttgacgtc gatggtcagc tggcgcatgt gaacatcggt cgggtttttg
    42421 aggttggtgg catccacaaa ggcaaaggtg atcgtcttat ctccctcttt gatcgcggag
    42481 gcgcgcatgc gcggctggtt attgagggag caatagtgcg tcaacatcag ttggtcgccg
    42541 tcgctgtagt acatggtcgt catcgggggg ctgtcgggcg gggtgagcgt ttcggtcaga
    42601 ctggtgcctc ccgaactgag cgcaaaggcg atcttgtggg ctttcccttc cgcgtcggcc
    42661 cccttccatt ctccgacgag gaactttaag ggactcaggg ttgtatctgg agtatcggcg
    42721 gcttgggcga agagcgggac gaagagtagg acgagaccac agagagcacg aagaattcca
    42781 cgcggcatgc aaaccctcca tcaaacaaga agatgccgaa cacaagtcag tcaagcatgt
    42841 ctccaaggtc gagatcaaga ctttttcagc cgctgtcgca gaacgggaca gcaggaacgg
    42901 cagagtgaat cagaaatgca cgtataatga cttggagtga ggggaaagac aggaggcgcg
    42961 ttcgggcgag aattgccggg ggagtgggaa cgtccgccat gaatttgata gaacaggtct
    43021 ggcgatagtt ttcctgagac acgaacggca ggccggccta tgccgcacaa ctccagcgca
    43081 tcgatctcga aggcgagtca cctgcggtcg tggtgcaggc gcttcaagtc acgccgaata
    43141 acctcaccgt gcgtctccat cgtgcgcgcc aggccctgcg ggccagcctc gaacagactt
    43201 gcgggctttg caccagacaa ggctgtttca actgcacgtg cgagtagttc ctccgcctac
    43261 cggtagatgt gaaaagaacg gtcggcgccc tgtaataatt cccgccttgt tccgtctgta
    43321 ggggtgaacg gatgatttca ccactcacag ggaggacaca catggctcac caccattcag
    43381 cgactatcac cgtgccgcgt cacgcagagc cacgaggtca cgatcaccat acgaacgggc
    43441 atgactgtgt gcaggctcac ggcgatcacc gtggccgtca tcagcctcac gagcatcagg
    43501 aagcgaacca ccaggcatcc tcgctcgatc gaacagcgct gatggcgacc ctgcactgct
    43561 tgagcggctg caccatcggc gaagtgctcg ggatggcgat cggcacggcg ttgggctgga
    43621 gtaattggcc gacggtcggc ctcgctgttg cgctggcctt tctcttcggg tatgccatga
    43681 ccttgtaccc gttgcggcgg gcgggtatgg cctggggaac ggcgctgggg ttggcctttg
    43741 cgtccgatac cctctcaatg acgactatgg aactggtgga caatgcgatc atggtgatgg
    43801 ttcccggtgc gatggacgcc gggttgcctg atccgctgtt ctggggcagt ctggtcttgt
    43861 cccttcttct ggccggagtg gcggcatttc ccgtcaaccg ctggttgatc gcgcggggta
    43921 aggggcatgc gctggtgcat gggcagcact gccactgagg cggttcggct gcaaccgtcg
    43981 atccgctgct gcgacatgcc tggctgctga tgaggggcag ccaggcgggc gggctcgtcg
    44041 ttcagtcctt cgacgtactt cacagtacgc cgcagtccct cacgactccg cgcgcccgtc
    44101 tcgcgacgcg gcttgacgat ttcgcaacga accggcgcta gcccggatta ttcatgaatg
    44161 tcgtcgcgag gcgttcgaga ccgaagcccg ccggggcttc tcgcaagcca ggccgacgca
    44221 ggcgtacttg cagtccgtcg aggaggccga acgagaacaa ccccgccggg cgagagaatc
    44281 gaacgacgca gcaggtattc atgaatagtc cgggccaggg ttttgatcag tttgattgag
    44341 gagcgattca gcatgacgat cgggaaatat atgtgcatgc tggcgatcgt ggccacggcc
    44401 atggttgcct tcgcgctggc ggggtgcagc cagttcgagc cgcgcgacaa gcgcttctat
    44461 taccgggcgc tgtggaattt ttcgttgcgg gaagatctgg ccgaactcga cagtgagttc
    44521 aacggcgtgg acttcggcca ttccaatctg tacgagaatc tgttgctgac cggcggtcag
    44581 gacgtgccgg ccattgagga gcgggcgcgc aagcagacga tcgcgtttat cgcgaccaaa
    44641 ccccggctga acccgaacga ggaggcgatt gcgccgacct atatgaaact ggcctggcgc
    44701 gcgcagaaca cgttcgacga agcccatgcc ctgcaccgcg ccacgtacga cattgcggtg
    44761 tcggacgagc cggagaagga tcgggcgatc cgcaatgtgt tggcctacta taaggacagc
    44821 gcctacgcga tcacctcgaa gcgattggat caccatcggc tggaccagtt tccctattcc
    44881 aaggccttca ggacacgctt cccgctgttc aacgcgacga tctggtcgta ccactacttg
    44941 caggtggccg tgtacgaccc gttgcaggcc gcgcgtgacc tggccgccaa gacgcaagcg
    45001 gtccggccca ttcttgcgac ctatcggcgg tatctggaac agccaccggt gcagtggacg
    45061 ttcatgccgc tcacggcgga actgagtccg cagtttgccg cgcggtatcc cgaattggcc
    45121 aacatcttcg acaatctcca tatgctgcac gacaacatca gcgatatcct caccagcgag
    45181 cggctcccga cctgggaggc gaagcgggct gaaatttatc gtgtgctgaa tagctactat
    45241 ctggcgagtg cggatgcgac caatccgatg atcgtgcaag gacaggagca tcaccattga
    45301 agttgagaga taagcgattc cttgcgcgct tgctcggctc ggcccttgcg ctggtgacta
    45361 caccgctgtt cgccctggac catgacaacc tcgatcccaa ccgtccgatc gggatggagg
    45421 atgcctatgc cattccgaaa ggggagatcg gcctggaggg cggggtgagg ttcaacgacc
    45481 gtcgagaagg ccgcacgcag gtcacatttc agccgcaaat tatctacggc gcgttcgcca
    45541 atacgcagat cgaaatccag ggcgatctgt ttaccgaccc gcactccatc gtgggggcga
    45601 acaagtccgg cgatttgcac ctgggggtcc tctacaactt caatacggaa acgttgatgc
    45661 tgccggcgtt ggccgtccgg gtcgaggcgg atctacccac cggcgtgaac tccaaaggtg
    45721 tggacacgca gctcaccggc atcttgaccc ggtcgttcgg ccggctgcga gtgcacctca
    45781 atgcgggcta taccgtgatc gggtcgccgc aaggacagga gcggcccggc gcctatcggg
    45841 ccgtcgctgc cgtcagttac cctttgggct accccacgag ctttcgcgac acgctgattg
    45901 tcagtgtcta cacccgccaa tcggacctgc gcgggcaaca gaaccacacc ggcatcgaag
    45961 tgggactccg gcaccaattg acctcgcgtg tcgtactcga cggcgggctc ggcaccgagt
    46021 tcgtcggccc ggcggatcgt gcggcgctgc tggggacgat gggggtctcc gtcggatttt
    46081 aggggtgggg ggggaggcgg cgtgcccttc ctcgcatcgc acacctggcg gaacgcgcgc
    46141 cctcagaatg gcgtcgctcg aggagcgcag gggaaccagc atgagcatag ttgctggtgg
    46201 ttagagttat acggcaggtt tggagaaagt gttcgggatg ctgtgcgtgc aacgcgcgaa
    46261 agcaaggtcc tctgctactc gcgacctatt tgtcggtgga tgcttactct tgagcgcttt
    46321 acttccttat gctaacgtaa agcagaatgt aggaagcaac ggagggaggc accatggcgg
    46381 tccaaaaact cagcagcaaa aatcagatcg tcctcccgaa ggaggcgagg caggcgatgg
    46441 gcgtccgagg cggtgatgaa ttgctgatcg tcgtcaagga tgatctgacg ttggtcatgc
    46501 cgaaacccaa acggtatgcg cagactctgc gagggcttgc taagggcacc tatccgtcag
    46561 gctatctcaa gcgagagcga cggtcgtggt agcgggctcc acacaagggt tccccctcga
    46621 acgccaacgc atcggactcg ataccaacgt attcatttat ttcttggaag accatcctag
    46681 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncctaggtat ggggcatggt
    46801 gtgcctcgtt gttcgatttg atcgagcgtg ggctcaatcc agctgtcaca tcaaccgtga
    46861 ccctcctgga gttgctggtc cagccgtacc gtgaccagaa agatgagttg gctcaacgca
    46921 tttttgctct tgccggcacg tatccaaggc tggaatgggt ttcggtcaca atgaatgtgg
    46981 cggaccgtgc agccgaactc cgtgcccggt atcgtctcag cacaccggat gcgatacagc
    47041 tggccacggc catcttgcac aaggccgtgc gcttctacgg gaatgatcgc ggtttgcgaa
    47101 aggtgaaaga gattgagtgt gtccttatag atgagttgat ctgacttcgt agactcctac
    47161 cccgttgccc cgttccgccc aagcctttcc atctggccct gcgattttca acgtagtgtg
    47221 aggctgccct atcggcaatc ggaagccgct ccgatatgtg cttccgcaag cctctcgcgg
    47281 taggaaaaag gtctgaggca tgctgtccaa ggttgtggcg cgaagaccgc ctttcgaccg
    47341 gtgctaccgt gccaggctca gtttcgccat gtgccagaga ctctccgcca accagcggct
    47401 gtctttgaac agatgggcca gttcggacgt gaggatgaag aaagcggggt aaaacaggtg
    47461 cgggctggtt ttactccagg ccagggccgg ttccacgagc ttcacaaaga cagcacgcca
    47521 tttccagatc acggccacgg ccgtcacacc gaggcacgcg ccggacagaa tgtccgtcgg
    47581 aaagtggaga ccgacataaa ttcttgggat ggatacaatc accgctgcgt ggagaaaggc
    47641 gaaggcgccc accagcgggg acaccaacca gaggccggcg gcaagggcaa agaagagtga
    47701 ggcatggtcg ctgggaaagg aactccaccc ggccaggaaa tcggccggca tggtgtaggg
    47761 cagggtgaaa tccaatccgg cggtgtgcac cggacgtgcg cggtagggga ggacgagctg
    47821 aagtacccgg gcgatcgcca tcgccgcgaa ggccgcgagg atcgtcagga ggacggtctg
    47881 tcgatggtcc tcacgatggg gagcgcgaaa ccaggcccac cagatgagcg gcagagagag
    47941 ataccccttc aacgcatcgc tgtcgctgat cgtcatgatc aggctgtcca cggcccagga
    48001 ccgttgagca aactggttga agaaacctaa gacggcggtg tcgaatccgt tcatgtgcgc
    48061 gtcatctgca gagtggagtt cgtatcacgt gaatcagtgg tcatggggct cggctcatgc
    48121 cggagcgcag gtgtgcggca acggcaagat agcggaggtc agggctgttg taaatgctta
    48181 taagccagac cggtagaaaa tcgaaaaatg ttttcgtcat tcgagcgctc gacgtgaggc
    48241 tcgtgtccgg cgccggccag ggccgatgtg ccctggtcaa agccgtcctg gaaggattgg
    48301 atctgcccgt ctctttggat cgatcagcac cttcttgacg gccattcggc cctaggcaga
    48361 taatgcgccc gtcgccagga ggcaggcatg ggacgggccg gccggatcgc acgcggaatc
    48421 atggtacttg tcagtgtcct gttggtcgcc gcttgcggcg attcattcaa tgacgcgtat
    48481 tgcgaagcga acaaccaccg caatcagcaa accgtcgaac acctcaccaa cgtcctcgag
    48541 caaaagcgca atcagcccgg actctatctc gcgcgtgccc gctgccacta ttttctcggc
    48601 gcctatgcgc aagccctaca agacgcgtcg gaagtcattc gccgtctgcc caataaagcc
    48661 gatgcctacc tccttcgcgc caaggcaggg aagatgctcg gccagattcc gcaggcgctg
    48721 caagactaca gcgctgccat taaattccag ccgaccgccg aagcccatta tggccgtgga
    48781 ttgacctacg tgcgggaata tcaggggaag gatcgcgaag ccctggagga tttcaccgcc
    48841 gccattcgcc tcgatcccaa acatgtcggc gcccatgcat tccgcgctca gatctacagc
    48901 cgccacgaac agtttcagca cgcactggcg gattcggaga cagtgctcaa gctcgatccc
    48961 accaccccca acgcctactg caatgtcggc tttgcgcact atgcgctggg gcatgacgcc
    49021 gagggccgca agtttctcac cacctgctac cagaaggatc ccgatcccca gacgcgcggc
    49081 tattacgaaa cggaagtgaa tagggtgctg tatgcccgga agccgaagcg gaattccggc
    49141 gggaattatg tcgctgaagg tggggtcaag acgccctacg atcgggaaat ggaacgccag
    49201 cagaatgtgt acgagagcct gcgcaattca gggttcaacg accgggcgga ggcatgcagg
    49261 acggatgggt cgaaatgttg agagaggggc gtcccgagtg agcccccttg ctcgcgcaac
    49321 gcgcggcctg agaaggccct cgttggacgg cgcgcagtgg ggg
//
LOCUS       DAOQRM010000004        46181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_380_length_46181_cov_17.957681, whole genome shotgun sequence.
ACCESSION   DAOQRM010000004 DAOQRM010000000
VERSION     DAOQRM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 46181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46181
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_380_length_46181_cov_17.957681"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..884
                     /locus_tag="PKK81_00785"
     CDS             108..884
                     /locus_tag="PKK81_00785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV31430.1"
                     /translation="MTVVTRPWASVVIPIKDERENLVPLTEQLVTVLDGRGESRSAPF
                     ELIFIDDGSSDGSSDVLDGLAARYPTVKVFHFDRNYGQSAAFDAGFKQSTGDLVMTID
                     GDLQNDPADIATLLPHIKTFDLVCGWRKDRHDNLTRKISSRIANSVRSAVTGDRVHDT
                     GCSLKLFRRPVVEKMQLFEGMHRFFPALALMHGFTVTEVPVRHYPRTRGTSKYGVGNR
                     LFKGLYDLVAVRWMQHRCLRYQYRAAATTSSPSASAPTRL"
     gene            881..1180
                     /locus_tag="PKK81_00790"
     CDS             881..1180
                     /locus_tag="PKK81_00790"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNV31431.1"
                     /translation="MTLDTIWLIIGFLGQGIFFMRWVVQWIASERHAESRVPTAFWYM
                     SMIGGLITLAYAIYRQDPVFIAGQSIGSIVYLRNLMLIHRPNQADSGTASPATKS"
     gene            1192..>2945
                     /locus_tag="PKK81_00795"
     CDS             1192..>2945
                     /locus_tag="PKK81_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNV31432.1"
                     /translation="MNGQHPSSQTPAQADRSIQPLALILLLALAAVLFFVGLGSLGLT
                     DRDEGRNAEAGREMYETGNYISPTFNYEPRFAKPVFVYWLMSLSYHVFGVSEFAARFP
                     SAIFGVGLILLQYLFLTRCRGPVVGLFGAAMLLLNLEIIGLSRMALTDSVLIFFTTLS
                     LYGFWLGLYGEGRERHYLWFFYIGMALATLTKGPIGFLIPMLAVGLHLWLTRSWGLFW
                     RHGFPIPGLLLFLLLALPWYLIMLNIHGQRYTTSAQGDTIGRFFGTMEGHGGTLVFYL
                     PVFLLGFFPWSGLLPFAWYQAYRSWRDSKRSGALPPSQTSVLDVHPSPHALEWFTAAW
                     VLGGLVFFSLSSTRLPHYIGPLFPAAAILTASYWNRCVTDQATPGLRAAIHTITAVGC
                     ILALAFASLPPLYAKFAGKLIDEFPLAGQVTLGPGPYTVASIFLVGMGLIAYFGFSET
                     RRPAVFWVAGGSLALVVLATTQLIFPLVHHFVIEPPQQLAEVAGVNLGPNDRLILYGQ
                     PRPSLVFYAKRKAIVVPKNEEANIKPYLTQPGRTMILLPAAFRNRLPFETMDYPVLLE
                     RYGYILLANQSLIHVPEEA"
     assembly_gap    2946..3045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3046..3865)
                     /locus_tag="PKK81_00800"
     CDS             complement(<3046..3865)
                     /locus_tag="PKK81_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNV31433.1"
                     /translation="MIEPGDGQAASQPAQPEPPPACPSIPAVLLSVIVLFGSFAALFY
                     IDIPILWFLRSHNLSALQSLGDLGEKLGNGGTLVTISLVLLGAGYMLKRQALTRVALD
                     SLLAHGAVAILVNGLKHIIGRPRPRLTHSGGWQWWPSLDSGLDSFPSGHTSATVAVAT
                     VLARALPRYRWVPFVLAAWVGASRVWRGSHFPGDVVAGMVLGFVVGSIFNGPLRWWGR
                     SCGQALIRITPVVLSLTGLFWVLTHRIVDPMTDHILLASGAMLVAGGMALRMAGR"
     gene            complement(3862..4995)
                     /locus_tag="PKK81_00805"
     CDS             complement(3862..4995)
                     /locus_tag="PKK81_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31434.1"
                     /translation="MKHVKTVMIGMVGLALVATSTVLAGAAEPKSAQAPANASASAER
                     HLTNIRQLTFGRQNAEAYFSFSGNKLIFQSTNNWMKDTFAAAMHPSDIPLGCYQMYVM
                     DLGSEKIRMVSTGAGTTTCGYFFPGDRRVLYSSTHLRGPNCPPKPKRDGGAYRWALDD
                     YDLFSVRIDGLDPQRLTNTPGYDAEATVSPNGKTIVWTSMRDGDLDIYAMDLDGTHPR
                     RLTQEIGYDGGAFFSPDSKRIVYRAQHPSNQEELDQYKALLAQNLVEPGRLEIFIMNA
                     DGSNKQQVTNNGASNFSPFFHPDGHRVIFASNVDTRNASGRPEFHLYLVNEDGSGQER
                     LTFEGQFNSFPMFSPDGKTLVWVSDRHAKEPGEFNVFLADWVP"
     gene            complement(4982..7009)
                     /locus_tag="PKK81_00810"
     CDS             complement(4982..7009)
                     /locus_tag="PKK81_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNV31435.1"
                     /translation="MTVAGGFPGQELAFSLAPTLLLERVEDLTHCQDPCEFAPSVPFA
                     RDRSSSQAESQRILLKQPPANQAGGSIRLKFVYHGVIDDPPRDPRHLRFVTPSETAGH
                     IGPEGVYLSSESQWYPDLDDSLATYDVRITLPEGWAAVTQGTAQADASRWIVSTKSEA
                     LTVVANRFVVKTRTWKAQSGQAIQLATYLFPDDAALADEYLDASARYLDAYIPLLGAY
                     PFSQFAVVENFFSSGLGMPSFTLLGSGVIKRHYTQPYALGHEIVHSWIGNGVFNRVSR
                     GNWVEGLTTYLSNYYYHELTGDEAQAREQRRLMLLGYSVYVRPGEGYPLRRFAQKSDE
                     KDNAIGYQKSAMVFHALRQEVGDAAFWRALKRIPEQYLGTVADWSDLERTFQEEAGRD
                     LRWFFAQWVERAGVPELALSGLRLQTVSGAFGHANAVEAAIHIAQSDEPYRAPVELEF
                     VLSGERTHRVRVQLSAAQEDVVVPLAEPPSAVRLDPDYHLFRRVARSEMAPVLNLYVT
                     DGQRSVVLAQGSPAQPGPFGDILQRIVAQESAKPDAVRTIVLQPGEGNRSVPSGSLLV
                     LGDPRENPIAAAAVRSCGDRVRFLDGGFSVAGKMYEGAAMALLVSCRREEHPGSVVTL
                     LYGVTPQALGRVARLLFFYGWQSYVVFQEGAVVARGDWEDRMNTEVRIETR"
     gene            complement(7205..7993)
                     /locus_tag="PKK81_00815"
     CDS             complement(7205..7993)
                     /locus_tag="PKK81_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31436.1"
                     /translation="MMSTLLVIDDDRLHCDLLQMALARHGYQVSIATSGCEGVVLFQQ
                     LRPLVTLLDLRMPEMDGLAVLKEIRTHDPRAGVIMLGGGATEELENQARELRVTDFLR
                     KGLSLDVLIGAVHRVAQQARRQASSEQSHAGGEIDQLPDEQILVVDDDVMARDLLVRF
                     LSLRGYHVRAACDGREALRLIGESAPDLLILDLAMPEMNGVEVLRTLTARDYAGRTII
                     LGGHQNDPLLAEAWALGPQEVLDKPIDLERALMAIQLVMVCREC"
     gene            complement(8197..9111)
                     /gene="ftsY"
                     /locus_tag="PKK81_00820"
     CDS             complement(8197..9111)
                     /gene="ftsY"
                     /locus_tag="PKK81_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNV31437.1"
                     /translation="MDWLQKLSAGLSKTRDAVTGQLDRLLGRAADPALLDELEVALIS
                     ADLGMPVVERVMGQLRAQMRGGNFSSADKVRDLLRQSVLDILLPTQSASMEQLVAQGP
                     RPFVILAVGVNGVGKTTTVAKLTQRFRQQGKKPLLVAGDTFRAAAIDQLQVWADRIEV
                     DVIRHRPGADPAAVAYDGMTAAKARGSDVVLIDTAGRLHTKTNLMDELRKIKRVVAQE
                     CQGAPHEVLLVLDATVGQNAIAQARQFHEAVGVTGIALTKLDGTARGGIVVAIADTFK
                     IPVRLIGVGEAVDDLQDFDAKAFVDALF"
     gene            complement(9200..9670)
                     /gene="ybeY"
                     /locus_tag="PKK81_00825"
     CDS             complement(9200..9670)
                     /gene="ybeY"
                     /locus_tag="PKK81_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNV31438.1"
                     /translation="MPVVIGMRLTRWPLRLGALNQLAMHVLQAVGEAETLLSLEIVGD
                     VRMRRLNRTFRHRDKTTDVLAFATREGPGPPSSLLGDVVISLPQAIRQACGHEQGVDH
                     ELAVLLIHGILHLCGYDHERSDAEAQRMSRREKAVLRAVAPVPRLLVSRGSDQV"
     gene            complement(9670..10731)
                     /locus_tag="PKK81_00830"
     CDS             complement(9670..10731)
                     /locus_tag="PKK81_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNV31439.1"
                     /translation="MRKIKLREGTNTAALFGHHDRHLKLIEEEFGVRCSARGEEVTVE
                     GTPETVKQAERVLNELASLTNEGYDLRSDDVTHALTALRHNHDASLKELLFSASPIVT
                     RKRFIVPKTPTQKYYLDAIEKHDIVIGIGPAGTGKTYLAMAMAVSALMKKDVSRIILA
                     RPAVEAGEKLGYLPGDMYAKVNPYLRPLYDALFDMMDMERANRLIERGDIEIAPLAFM
                     RGRTLNDSFVILDEAQNATAEQMKMFLTRLGFHSKAVVTGDITQIDLPSDRVSGLIEV
                     RDILQDIAGIEFVYFDERDVVRHRLVQEIIKAYDRHTASPAPPAQPRKGEAQTKGHRS
                     DSKPTRPAAPSTGSWGQSH"
     gene            complement(10847..>11269)
                     /locus_tag="PKK81_00835"
     CDS             complement(10847..>11269)
                     /locus_tag="PKK81_00835"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNV31440.1"
                     /translation="ENVLDTTGFGRKDIVAVEVVARDLDATATPARSVPIVLGNSPPQ
                     ILSSPAALTNREQYEYVVQAKDVDGDSISYGLETGPPGMTIDKATGHVTWKVTPGVGG
                     THRIKIMVEDGQGGTAWQDFELSIPSTAQSLTVLPTQG"
     assembly_gap    11270..11369
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11370..15775)
                     /locus_tag="PKK81_00840"
     CDS             complement(<11370..15775)
                     /locus_tag="PKK81_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31441.1"
                     /translation="MNRRTFVAVMLVAGSLSAALLGSRDAVAVVSNADYTAVPAFVSN
                     ATTPNIIVVMDNSGSMSNRACESSSCGTLADGSTSTTTTWTNTTRYSGYFDSLRCYTY
                     NTTDTRFESGTGKTALATACPDTEWDGNFLNWATLRRFDAVKRAMIGGDCTSTRAADG
                     TCPTSGTPALKTVRAQGAGLSNERADVNYGGGTGANTYVGRIPLADRGSDPATISIGL
                     SASGTPAAPYFCVDNDTTFNSNCGDSYSVRKYELKVGYSSEPTGVIQQIGSKARFGLV
                     EFKSSSEGARMLVGAGSRQSIDWADGDVETFTTNTAAMVDAVQESYPSTWTPLSEALY
                     ETARYVAQIQSTFATGYVYPIAFSGGISNGVNFATSGAGSIGTSEITALVGTETCPAG
                     YITSACGRDPYFFGQNHTPPWSASSQVVACCRTFVIVFTDGEPTQDQNIPTALQDYAH
                     AHHGQHCTGSDGAAPPRTIDGTCNTHAATPFSDLLAEHKTSYADSGSHYLDDVAYWAH
                     TTDLRPCSGTTDGTIAGLTVTGHCIAGTQSLTVYSFFAFGNINGRGILAQAARLGGFE
                     DSNNDGVPQTNEWDKENNLTGAATPDGIPDAYFESSNVDDLQDKLLATIASILRRSAS
                     GSSVSVLATASTGEGALYQSYFFPSTIEPSTLSDVKWTGYTQALFIDTFGNTREDTNQ
                     DGRLDYKVDKIIKTRFDSASNSVKVDKYVDSDGDGLPNDQNSDGVVTTADCNPCGQAL
                     SDILPIWEAGKQLALKDSTTRTILTWVDSDHDGVVDSGEQLAFTTANEPTLRPYLRAA
                     SSAEGTKIINFVRGCDVGTCAEQATTRDRRLQVPAGSGSLKVWKLGDVIHSTPTVVAG
                     PKDRHDAIYGDQSYSAFLQKWYNRRQVAYVGGNDGMLHAFNAGYYHPGDDASASAPAN
                     TTEHGWFTTAPADNSTGAPLGDELWGFVPYELLPQLEFLSRADYQHTYYVDLPLKVAD
                     VRIFTPDTDHPNGWGTILIGGFRMGGSCGACSAGTGAPPMTVNISGTDYTFYSSYFAM
                     DITNPDAPKLLWSFSSSSLGLTTSAPTVVRVNPAADAKASNTNAKWYMVVGSGPTGYD
                     ASVVQSAKVFVVDLATGPGSGNSLVTSYSVGSWNSYIGDITAFDRDLDYRHDVVYFGR
                     VIHDGALPWRGKMYRLTMGAGGATAKFGTVTSPTQWGIASGSARVPTEMLDTFVSGAE
                     MGPVATAPSVTTDDAANVWVFAGSGRYYSKSDKTDVSTQYFVGLKDSVLNSGCNQSAG
                     ALNCMDNDLVDVSNATVCVVGVGDCGQASGTNQVSGVSGATTFTGLIGLVQSKDGWVT
                     KLVEPANPPTNPVPYGIGERAVSSPTVFGGVVFFPTFIPTNDICVSSGTSRLWALFYK
                     TGSAYQEPIIGTTTSGANQTVNKFGSLGEGLAFGIVVHMGSGRDGGSPFGLLINMSQG
                     NFGDCATCGFGGGSPGGLPSSNIGANVAIDPR"
     gene            complement(15853..16182)
                     /locus_tag="PKK81_00845"
     CDS             complement(15853..16182)
                     /locus_tag="PKK81_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31442.1"
                     /translation="MNNTAFIVVLGLLTLGQTMPSVPSISLAETAQGPEAMLFSTVGF
                     QSGVIDVVQGASIRIDGRSYVLKSAVVVVNHEGEPLEIERIIPTSLVKFHLKEGHIDK
                     MVVTLPQ"
     gene            complement(16199..16831)
                     /locus_tag="PKK81_00850"
     CDS             complement(16199..16831)
                     /locus_tag="PKK81_00850"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HNV31443.1"
                     /translation="MNLQQGMHGAGREGGGAGLNNQQGIALLTVMLMLLILTILGIAS
                     ITVTSMENRMAGFFRTTEAVVAAADSCEGLAANIIQQTLSPPGVLPASFIAPTGPVPT
                     ANATTLYAEIYGYDLPSPAPANTPAVNYADVASTAPNFVMTNLPGFTVNGDIDLLYTH
                     TKSGQGTGVAGTEHVYRITCMASNPATGSNSTVTSVYGCLDGDTCVKKIN"
     gene            complement(16846..17949)
                     /locus_tag="PKK81_00855"
     CDS             complement(16846..17949)
                     /locus_tag="PKK81_00855"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNV31444.1"
                     /translation="MRRFDWNQQGFTLVELMAAVLITVVIVAATMTTVVTSNRANVVN
                     TQVADTQQNVRLAIDLLSRDIKLAGFNYNATDPATGSVGACNATIGAVVKPVGLLPQD
                     QTPTGADTGTDSVSMVLPVMNTTGWTLTAAVGGTPNNKTYDNSISLSGAVMTEMVAQG
                     LVVGSTISIGGALSKTVQAVNATSIGFGTGNFVDGQFPVGTPVYLMQCVRYQVVANTP
                     ATCGSDTPCLVRNNVPLVDGVEDLQLAYACDGCNQAVPNPLFPDGVVDDQDASSTSGF
                     PTFTQGDFVSNSAWAIAPWTPDKIRLAQVSLVVRPTNADDGLDEKGTKAVNTTGPVIV
                     GDHNPSADAGYNASTYMQQRRRVMIRTIQPRNL"
     gene            complement(17970..18494)
                     /locus_tag="PKK81_00860"
     CDS             complement(17970..18494)
                     /locus_tag="PKK81_00860"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV31445.1"
                     /translation="MKHRQNSHPCFSLLAAAQRGFTLLEAMIAAGVLSVGLLGLAGLS
                     GMSLGKNVDANDMSRVTNIAADMAERIQNNRQRVLDYHNTNTSVACAQSANTQRMALG
                     DCTQWQALVANSGLTGATGLVTAARLDPDPTANPVTMNRFLVTVTVSWQTRQTDVSTA
                     RTKTAIFTTVVAPE"
     gene            complement(18859..18978)
                     /locus_tag="PKK81_00865"
                     /pseudo
     CDS             complement(18859..18978)
                     /locus_tag="PKK81_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            complement(19357..21156)
                     /locus_tag="PKK81_00870"
     CDS             complement(19357..21156)
                     /locus_tag="PKK81_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV31446.1"
                     /translation="MSRATVSMLPVSECSQAVSPLAQTRSAACYHRLVEQGFLQQEEL
                     VLATAEAARKRLDVATVLMERYRIPKPALGAALAEFYHCPFLAYDERTVMERELLKNL
                     SLDYLRMNHWVPLRRQGNGIEVLIDDPHNPDKLFDVRRAFPGQALSYRVGLRCDIARL
                     LNAIQGRESGDAISDILGELVSEAQLEEQQNATIAAITENDSAIVRLANQIITDAYRR
                     GASDIHIEPYADRKETAVRFRVDGTCFTYMKIPAAYRRAIVSRLKIMASLDIAERRKP
                     QDGKIRFKLGTGQEIELRVATLPTSGFNEDVVIRLLTAHGARRLEDLEFSDGTRRLVG
                     QLAQKPHGIVLCAGPTGSGKTTTLHAILASINTDERKIWTAEDPIEITQDGLRQVQVH
                     PKIGLTFATTMRAFLRADPDVIMIGEMRDKETADIAIEASLTGHLVFSTIHTNSAVET
                     VVRLLDLGCDPFNFSDAMLGVLAMRLCKRICPNCREAYHPTRSEYDELVQAFGEGDWE
                     GVHPAYNPGLTLFRGRGCDTCNQTGYRGRVPIHELMVVSDSMKALIQARARTGELLTL
                     AKSDGMRTLVQDGIEKVLQGLTTYKQVRAVAIK"
     gene            complement(21261..22736)
                     /locus_tag="PKK81_00875"
     CDS             complement(21261..22736)
                     /locus_tag="PKK81_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31447.1"
                     /translation="MGRSRIAVRRDERGMALLAVLMVVFLLTLLGMTSMQLAGQEIVG
                     ASALQEERLAHHAAEAAVDVVMGWFHDPALHPQGVETTWLTKRLVNAQGDASYFDAQG
                     RAQFAGTGASPDVVFDAAHAQHDRLMNDPQTGWFKSLKGLARILKLRVYGATRPGLLC
                     TVEVTAGSGHTSRVTKTVSVEFGAYAVPALHAPIQGGTVGNESAPSSAGSVFAHWGDM
                     VVRGKAYFPRPEAVPMKSGLAPVTGQSYDEMTHREDRWFTIRLGGDAFFAQLPAEAWS
                     GVPLNLHTHQDPVPGLKVDQWDYDTLKRMARQFGQYYGIDRDGLLYAGGVIQPGAGHP
                     ASEVFASKGPGDHRGLIFVDTLDGMPPRPDNLGTIVLDQEYAEGIFIVNAHVLWKAGA
                     HGKPVPALSPPPEGQQSLGARIPVQLSGIHLQGVLCVAGDVRYAGHLKVYGGVVAQGA
                     IVDGTNGSGMLEAWYNHDLRDGLVQGMPLVFVAPGSWQAKI"
     gene            complement(22767..23357)
                     /locus_tag="PKK81_00880"
     CDS             complement(22767..23357)
                     /locus_tag="PKK81_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31448.1"
                     /translation="MVTNQDLRLGLDVLCSEVRLAGAGLLGEDSPFLKAKSDEIEFFA
                     NLSGASTTLTQVAEIGRQDLPVEDGAGWPKGKQLLVCTAVHCAWNQLAADGRKQTLTL
                     VTPTAEQFPVRSAIFLLNRVRYYVKRQDDGAMRVMRDVDGGASTLLGDVSQFDLQYFD
                     RDGRVTTDLSEVVRVRLAIQAGRQGSRLVRDVAIRM"
     gene            complement(23540..24022)
                     /locus_tag="PKK81_00885"
     CDS             complement(23540..24022)
                     /locus_tag="PKK81_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV31449.1"
                     /translation="MSARGFTLVESMVALVVLSIGVIGTIGMCEWAERGLQRGALTTT
                     ALALAESRLEAKRSVAWERLLMDDVDQDGIVESVMHDDGLQDDQTNGDGIFTASATQS
                     NIRLVWTVELNRGHEPAGASLATIEARAIFRTMGGQEKEVRVRTIRANPRYVGLSVLS
                     "
     gene            complement(24064..24498)
                     /locus_tag="PKK81_00890"
     CDS             complement(24064..24498)
                     /locus_tag="PKK81_00890"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNV31450.1"
                     /translation="MNERGGSLVELCTVVAIIGIVVGVSVPGWAALVAKHQQRAVMVE
                     IASELRMARQLAMARHERIRVVVDAEQSELRTECMDCDTSLLRRYEFAHKGTAVESMT
                     TKPEIMFQPSGRSATATTMVLVDGRRATHQVTVSITGRVTLS"
     gene            complement(24790..25563)
                     /locus_tag="PKK81_00895"
     CDS             complement(24790..25563)
                     /locus_tag="PKK81_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNV31451.1"
                     /translation="MISYVTVLLFGAVIGSFLNVCIYRLPREESVAWPASHCPSCRQA
                     IAFYDNIPIVSYLLLRGRCRACHAPIAIQYPVVEAANAIGYVLVFWTFGFAPAAWAYA
                     ALASALIVVTGTDLSHTMIPDAVTLPGIVIGLLCAILILPIGLVDSLLGVLLGGGILW
                     FLAWISPYVFGKEGMGGGDIKLMAMVGAFLGWQPVLLAIMIGSFLGSIVGVGLIAIGV
                     MRREQYIPFGPFLAVGSILALLFHQPLLEWYWALIDIPQ"
     gene            25778..26068
                     /locus_tag="PKK81_00900"
     CDS             25778..26068
                     /locus_tag="PKK81_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNV31452.1"
                     /translation="MYMSPVAKTTKSRAKVSAPDPVLPPRKRRPEALTTREQEILELI
                     WTGFKNKEIAQRLKISVKTVEAHRANMMKKIRVSNTAQLLKAAIQGKMLKLR"
     gene            complement(26086..26862)
                     /gene="rlmB"
                     /locus_tag="PKK81_00905"
     CDS             complement(26086..26862)
                     /gene="rlmB"
                     /locus_tag="PKK81_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNV31453.1"
                     /translation="MAPVDGNDNSLEVIYGLHAVREALRAGVRPIQRLLVLGTDRQFG
                     EIARLARDQRVPVRVEPRAALDRLVPSGHHQGVVGVVAAKAYAEPDDILASAQAEGQT
                     PLLVLLDGVEDPHNLGAILRTAEASGVQGVFIPERRAVGLTSVVAKASAGAVDYMPVG
                     RVPNLSRLIERLQGAGIWVYALDAEAPKPYTALDFSGPVALVFGGEGTGVRPGVRDAC
                     DEAAHIPMMGKVSSLNVSASAAIVLYEALRQRREGAGKKP"
     gene            complement(26837..28297)
                     /gene="cysS"
                     /locus_tag="PKK81_00910"
     CDS             complement(26837..28297)
                     /gene="cysS"
                     /locus_tag="PKK81_00910"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNV31454.1"
                     /translation="MLRVHNTLTGSKEPFEPLVPGKVRMYVCGVTVYDYCHIGHARSA
                     LVFDVLRRYLEYSGFVVEFAKNFTDVDDKIIKRANEQGVSCEHVTTTYINAYYEDMEK
                     LGVRRATLEPRATEHIADIVRLVDTLLAKGMAYRVDGDVYFQVDRYPVYGRLSKRNVD
                     DLQAGARVDVDERKRHPMDFALWKGSKPGEPSWDSPWGPGRPGWHIECSAMAMRHLGE
                     TFDIHGGGMDLIFPHHENEIAQSCGATGKEFARYWVHNGFVQINQEKMSKSLGNFFTI
                     REIFQKSEWSDTVTGEMLRYFLLSTHYRSPLDFSDQSLNEAKNALNGFYDLFERLNES
                     ASAHGAADQQMQESTMRARAAFVAAMDDDLNTPNALAALQTLRGEANKALEVGLSGEM
                     RRVVRQEFRALGIVLGLLQTDTWRFKSQRQQPSSGGAEASTDTLSDEDIADKLAARLA
                     AKRSKNYQLADQLRAELASHGITIEDRPDGTSRWKR"
     gene            complement(28307..28942)
                     /locus_tag="PKK81_00915"
     CDS             complement(28307..28942)
                     /locus_tag="PKK81_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNV31455.1"
                     /translation="MTGLIEWLIEVLGRFVIATISTFGYTGIVITMAIESACIPLPSE
                     IIMPFSGYLVSTGQFSMMGVTLAGAIGNVLGSLVAYYVGVWGGRPFVERYGRYVLVSQ
                     HDMELADRWFAKHGEAAVLIGRLLPVVRTFISLPAGIARMDVKKFVLYSFVGALPFCY
                     ALAYVGLKMGEHWNQLRQYFHHADLVIGLCLAVGLGYFLWSHWPKRRPSME"
     gene            complement(28939..29754)
                     /gene="surE"
                     /locus_tag="PKK81_00920"
     CDS             complement(28939..29754)
                     /gene="surE"
                     /locus_tag="PKK81_00920"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNV31456.1"
                     /translation="MARVRILVTNDDGITSPGIHAVAAALGALGEVWIVAPDRERTAV
                     GHAVTLHKPLRLTKMAPRVFMVNGTPVDCVNLALVKVLPGRPSLIVSGINRGVNLGDD
                     VMYSGTVSGALEGTILGIPSIAVSQEGNETFRFDVGAQYAARVAAEVLRHGLPPETIL
                     NVNIPNVPVRSIKGVKVTCLSRRRFNNPIVEKVDPRGRKYYWIAGTRQSWSRENDADH
                     EALEQRMVSVTPIHLDTTHHEVLEQFKAWEQPLSRPSARPKTVKPHPRRRAQA"
     gene            complement(29921..29997)
                     /locus_tag="PKK81_00925"
     tRNA            complement(29921..29997)
                     /locus_tag="PKK81_00925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29961..29963),aa:Pro,seq:ggg)
     gene            complement(30057..30410)
                     /locus_tag="PKK81_00930"
     CDS             complement(30057..30410)
                     /locus_tag="PKK81_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNV31457.1"
                     /translation="MGNEPRLGSKVFYKIGEVSKISKLPAYVLRFWESEFSFLRPKKS
                     RGNQRLYVQKDVDTVLEIKRMLYDEGHTLAGVKRYWARRGRVTVKKLSSRKELAQRVR
                     GDLQAIIRMIDSHSS"
     gene            complement(30452..30727)
                     /locus_tag="PKK81_00935"
     CDS             complement(30452..30727)
                     /locus_tag="PKK81_00935"
                     /inference="COORDINATES: protein motif:HMM:NF001401.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNV31458.1"
                     /translation="MRKADIANEIFKQVGVSKNDAADIVELVLNLLKGVLQKGDAVKI
                     AGFGNFVVRSKGARKGRNPRTGEEIGITPRRVVTFRPSQVFKKYVNS"
     gene            complement(30873..32462)
                     /gene="cimA"
                     /locus_tag="PKK81_00940"
     CDS             complement(30873..32462)
                     /gene="cimA"
                     /locus_tag="PKK81_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNV31459.1"
                     /translation="MPSLTAAQASALEIYDTTLRDGAQAEDVSFSVEDKLRVAQRLDE
                     LGVHFIEGGWPGANPKDIEFFRTVKTIPFKNATIVAFGSTRKASNSVRKDKNLQELLA
                     SGTPTITLFGKSWSFQVTDALGIALAKNLELIEDSIHYLRSKDRRVFYDAEHFFDGYK
                     ANPDYALQTIRRAIAGGAERVILCDTNGGTMPWEIREICRIVKQECPVPLGIHAHNDS
                     EMAVANSLVAVESGILQVQGTINGIGERCGNANLCSIIPNLQLKMQRPALGNKLTHLK
                     DVSGFVTEIANLMPNKRQPYVGDAAFAHKGGVHIHAVLKNAATYEHIDPAAVGNRRRI
                     LVSDYAGRSGLLEKVEAYGISLNKNHAKLQELVETLKERESQGYQFEGAEGSFELLMR
                     KAMGSHRPSFQLLGFRVIVEKKQEHGLLLSEATVMVKVGEVVEHTAAVGAGPVNALDH
                     ALRKALEKFYPQLREVKLLDYKVRVLSADKGTESKVRVLIESGDHKDKWGTVGVSENI
                     MEASWQALADSIEYKLLLADR"
     gene            complement(32592..33827)
                     /locus_tag="PKK81_00945"
     CDS             complement(32592..33827)
                     /locus_tag="PKK81_00945"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNV31460.1"
                     /translation="MALIVQKYGGTSVGTVERIHRVAERVERAQKDGHQVVVVLSAMS
                     GETDRLLKLAHEVTGAPDERELDMLLSTGERVTIALLAMELRGRGVNARSFTGRQVGI
                     HTDSAHTKARISRVTADRIKEALSAGVIPVVAGFQGINASSDVTTLGRGGSDLTAVAL
                     AAALKADRCIIYTDVDGVYTADPNIVPAARRLDKISYEEMLEMASLGAKVLQSRSVEF
                     AAKYSVPVEVNSSFKEGKGTLVTREDADMEGVMVSGVTGDRNQAKITIVGVPDRPGIA
                     ARVFGAVANANIVVDMIIQNVSQASTTDISFTVPKADLRNAVDLVQRLSEEIGARSVA
                     VTESIAKVSLIGVGMRSHSGVAAKMFEVLSREGVNIMMISTSEIKISCVIEEKYLELA
                     MRTLHTAFGLDRVSAPALG"
     gene            complement(33832..35082)
                     /gene="apgM"
                     /locus_tag="PKK81_00950"
     CDS             complement(33832..35082)
                     /gene="apgM"
                     /locus_tag="PKK81_00950"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNV31461.1"
                     /translation="MKYLILHADGMADLACPELGGKTPLQAAATPNLDQIAQRGEVGL
                     LSLPSEAGAAGSDVTSVAVLGYDPKKYYPGPGPLEAAGLGVTVGEQDVVFRCTMVTVR
                     GESASGSKDRATDIKKLGPHVLMEDATAGLIDTEQARELLEAVNEQLGSESIQFYPGS
                     GHRHLMVWVGGKSRATCLDPQPLVGRSIADALPSGDGADVLRKIMDAAFFILRDHPVN
                     EEREQEGLKPANCLWLWGQGRAAVWPPLPERYQIAGVVVSSSDVHRGVGVCAGLDAVE
                     LPLADGSDANEFTGCVQAAVQELAKKDFVYLHAGLSDDVLHATDVQDKVRAIERFDAQ
                     VVGPILAALATHPAYRLLVVCDPGLAKSHGAEVPPILYALCDSAAKPSAGVTKRFHEQ
                     DANIAGTAPRDATKLMLRLFPRGV"
     gene            complement(35101..36159)
                     /gene="thrC"
                     /locus_tag="PKK81_00955"
     CDS             complement(35101..36159)
                     /gene="thrC"
                     /locus_tag="PKK81_00955"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNV31462.1"
                     /translation="MNRWRGIIEEYRKFLPVTAQTPVITLGEGNTPLIRAARLAKKIA
                     PGIDLYLKYEGMNPTGSFKDRGMTMAISKAVEGGARAVICASTGNTSASAAAYGARAG
                     IAVYVLIPAGKIALGKLSQAMMHQATVIQVEGNFDQALTIVKELSATYPVELVNSLNP
                     FRIEGQKTAAMEICDDLGDAPAMHVLPVGNAGNITAYWNGYREYRAANQTTRLPRMMG
                     FQAAGAAPIVLGHIVEEPQTVATAIRIGNPASWQSALTAVKESSGAIDMVTDEEILHA
                     YALVASEEGVFCEPASATSVAGVIKLSQAGQLKEGATVVCTLTGHGLKDADTAIGISR
                     QPQTVKATRDDVARLLHV"
     gene            complement(36205..>37038)
                     /locus_tag="PKK81_00960"
     CDS             complement(36205..>37038)
                     /locus_tag="PKK81_00960"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNV31463.1"
                     /translation="GTSNYILSRMTSEGQRFDVVLEEAKRAGYAEADPTFDVAGIDSA
                     HKLTIMVSLAYGTPVNFKEIYTEGITGLTPLDIAYAKEFGFTIKLLAIAKYSDGEIEA
                     RVHPTMVPSASQIAKVDGVYNAIQLVGDAVEDVVLYGRGAGSMPTGSAVVSDVMAIAR
                     NLLKDAAGRVPPASYQPDQRRPLRMRPMEEITSLYYIRFMVVDRPGVLSQIAGVLGRY
                     GISISSVLQQGRKEGQTVPVVIMTHMAKERDIQNALREINPMSYISEPTTLIRVEGRD
                     E"
     assembly_gap    37039..37138
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<37139..37350)
                     /locus_tag="PKK81_00965"
     CDS             complement(<37139..37350)
                     /locus_tag="PKK81_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNV31464.1"
                     /translation="MKSEIGVGLIGFGTVGTGVARVLIENAELIRRRVGVPVTLVRIA
                     DLDITRDRGIAIPPGVLTTDIQQVLT"
     gene            complement(37347..38543)
                     /gene="alaC"
                     /locus_tag="PKK81_00970"
     CDS             complement(37347..38543)
                     /gene="alaC"
                     /locus_tag="PKK81_00970"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HNV31465.1"
                     /translation="MAIGDGFYRIKRLPPYVFAQVQTLKLEARQRGEDIIDFGMGNPD
                     QPTPPHIVDKLIEASRKAKNHRYSASRGITKLRHAITGWYKRNYDVDLDPETEAIVTI
                     GSKEGIAHLALATIGPGDVVLTPTPTYPIHMYSFIIAGGEVRGIELRQDSDFFDDLLR
                     VYRQTLPRPRILVINFPHNPTTAVVDLEFFKKIVAFAKEHDVIVIHDLAYADIAFDGY
                     KAPSFLQVPEAKDVGVEFYTLSKAYNMPGWRVGFCVGNREVVGALAKLKSYLDYGIFQ
                     PIQIASTVALNGPQDCVKEVVQRYQNRRNVLVNGLNRIGWPVALPRATMFVWARIPDP
                     FRHMGSLEFSKLLLREAKVAVSPGIGFGEGGDEFVRFALVENEHRTRQALRGIRKVLN
                     LDDQES"
     gene            38845..39558
                     /locus_tag="PKK81_00975"
     CDS             38845..39558
                     /locus_tag="PKK81_00975"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNV31466.1"
                     /translation="MARLGVNIDHVATLRQARGGTDPDPLTAAILVELAGADGLVVHL
                     REDRRHIQDRDLTMLREIVRTKLDLEMAADDAMAKIALSVKPDLVTLVPERRQELTTE
                     GGLDVANHRDRIQKIVDLLRDGGIPTSLFVEPSLDQIKAAHKIGAAYVELHTGRYSNA
                     KRSKEEDEEFEAITQAAKLAYKLGLGVNAGHGLTYKNVKRLAKLPEIVEFNIGHSIIA
                     RSVMVGLVQAVREMRELVA"
     gene            39627..41183
                     /locus_tag="PKK81_00980"
     CDS             39627..41183
                     /locus_tag="PKK81_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNV31467.1"
                     /translation="MIPIVTAEQMRALDQRTITEALVPSLTLMERAGAGVVTHLEARY
                     APLAGKSVIILCGKGNNGGDGFVVGRLLRRKQAQVHILLLASPEDLSRDAKTMYQRFL
                     RSAGKSAVTCPADVETIQQRLATGDILVDAILGTGLSTPVTGLYRDAIEAITAVGRPT
                     VAVDLPSGLHADTGAVLGAAVRADLTVTLGLPKAGLYSGSGIDCAGTVRLVDIGIPAA
                     FVEAIGSRLMLLTDTAARAALPPRRPSAHKGTYGHLGVIAGSVGKTGAAAMAALSALR
                     IGTGLVTAAIPSSANDILEAKLLEVMTLPMPETKARTFARSGLDRLLAFAGARDAVAI
                     GPGLTTHPETVDLVQELVKRIDKPCVLDADALNALAGKSSLLTECKRPPIITPHPGEM
                     ARLETEATTQSVNEDRLGTATRFARERGVFVILKGARTVIARPDGLAAICPTGNPGMA
                     TAGTGDVLTGMVGGLLAQGLAPWDAACAATYFHGLAGDLAAQHLGQAGMIASDLIQHI
                     PHALAPTTHT"
     gene            41260..41781
                     /gene="tsaE"
                     /locus_tag="PKK81_00985"
     CDS             41260..41781
                     /gene="tsaE"
                     /locus_tag="PKK81_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNV31468.1"
                     /translation="MLRSPKHTHRLGRCLGTLLQGGEVLALFGELGAGKTSLVKGIAD
                     GLLAEPTDVSSPTFTLIHEYRGRLPLVHSDLYRLTAAQLKDTGLNDYLDGHTVTAIEW
                     ADRWGDGLPSDRLDVHLSHRPPAARRAILTARGPAARRLLDALRSRLSANRPTRAAQQ
                     TRVQLNRPRRASH"
     gene            41778..42122
                     /locus_tag="PKK81_00990"
     CDS             41778..42122
                     /locus_tag="PKK81_00990"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNV31469.1"
                     /translation="MIRLILVGTLLLLALSFFLQNQEQEVTLRYFFGLRSASILIYKP
                     ILAAFGVGLLVSGILLFPAWVRGRIELRRKTKALQEAEVDLERLRQALDKAARRVGTS
                     SDIPAEGEPSDG"
     gene            42115..44763
                     /gene="mutS"
                     /locus_tag="PKK81_00995"
     CDS             42115..44763
                     /gene="mutS"
                     /locus_tag="PKK81_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNV31470.1"
                     /translation="MGDADGTPLMRQYRDIKQAYRDAILFFRVGDFYEMFQEDAVEAS
                     KLLSIALTSRDKSSETPIPLCGVPYHAATNYIAKLLRAGRTVALCEQVEDPKLAKGLV
                     RREVVRLYTPGTLIDSEFLPSTESNVLAAVYVRLRSAGTGRKEPSYGLATLEVSTGDY
                     WLSEFHGIQAEASLRDELARLEPKELLFPEDLSPDEQQWTADLTGPRCCAQPATWFDL
                     EQGRMTLQEQFRVHSMEAFGCHRLTLGIQAGAAVLRYVRETQPSAPLDHIRPPRVRQD
                     HDAMHLDSVTIRNLELVRPAGRAEDSSNQSTYTLLGVLDRTVTAMGSRLLREWLLRPL
                     VNSAAIEARLTAVDELKRQIDARVRLRTALRTVQDISRLCSRMSLGVANPRDVLALKI
                     SVSSLPAIQAELSGLDSPLIADLMSSWDNAQDLYELIEGAIEQEAPVSIRDGGILKSG
                     YHPEVDELRKASREGKGWITGLEAKERERTGVESLKIRYNQVFGYYIELTKANLGKVP
                     PDYIRKQTLVNAERFMTAELKELEERVTGADTKLTALEQALFEQLRTRLAEETARLQE
                     ISRRLATLDVVASLAETAALNRYVRPTVDEGDGLHIVQGRHPVVERLDLSGGFIPNDT
                     HLDLATSRLHILTGPNMAGKSTYLRQVALITLMAQMGSFVPASEARIGLTDRIFTRVG
                     ASDNLAGGQSTFMVEMTESAHILNCATPRSLILLDEIGRGTSTYDGLSIAWAIAEYIQ
                     DPQRLGARTLFATHYHEMTQLESLREGITNYCVAVQERDGKVLFLRKIIRGGADRSYG
                     IHVAELAGLPDQVIHRAKAVLAQLESSSSSGRSVQENQQSLQFDATLPAPHPMLEEVR
                     QMNLFSMTPLDALNRLAELQQRLLQE"
     gene            complement(44869..45543)
                     /locus_tag="PKK81_01000"
     CDS             complement(44869..45543)
                     /locus_tag="PKK81_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31471.1"
                     /translation="MKLQKQGWTFMAAVALVVGFASTALAIEAFQERFEWGDLSKPTT
                     LQGRVIILDPYDEAVWLNIAVFGGNAESGLFWQRIHPGKNLKFYPADKNVWEQLKSMA
                     RPHSGPAAAKEVPPGSPTTLVEFVAQEVEQNRRVISSVKKLADNAGEMGKPKSISSLR
                     LKECEGKTEVDAPCHAAKTLLNTSPKTPDGANLPLQYDVLLPNGKPLANLIPWTAKYN
                     QGGGHH"
     gene            complement(45604..>46181)
                     /locus_tag="PKK81_01005"
     CDS             complement(45604..>46181)
                     /locus_tag="PKK81_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31472.1"
                     /translation="LFLDTVIQRFSGHHFSKEGRVMNRVGILAALAVSSVVGLLTAGV
                     TLAADAPAGGGPSEVTTGSGKFFKGETTNTLKGRVWSQWADNPDTELLFGIQYWNTGD
                     PASGEGGGRSSTSMDVKPPDPLFTCCGWGFTGGTDKNNPYAGWHHAQTTVRLSVKDKS
                     LMDQIIKASQELVAMEVTLDGRTITGFKVLK"
BASE COUNT         9789 a        14575 c        12294 g         9223 t
ORIGIN      
        1 tgagacggaa ccctggacgg aaccgtttga ccgcccctcc atgcgttttg tagaatgtgc
       61 cccgtgcaca ccgataaacc gctgcgtgaa ggaactgttc ccgctccatg actgtagtga
      121 cccgcccatg ggcctccgta gtcatcccta tcaaggatga gcgcgagaat ctggtgccac
      181 tgactgagca acttgtgacg gtcctggacg gccggggaga gtcgcgatcg gccccgttcg
      241 aattgatctt catcgacgat ggcagttcgg atggcagttc agacgtgctc gatggactgg
      301 cggcccgata ccccacggtg aaggtctttc acttcgaccg gaactacggg caatcggcgg
      361 ccttcgatgc cggattcaag cagtcgacag gggatctggt catgaccatc gacggcgatc
      421 tgcaaaacga tccggcggat atcgccacgc tgcttccgca catcaaaacc ttcgaccttg
      481 tctgcggctg gcgcaaagac cgccatgaca acctgacacg caagatttcc tcccgcattg
      541 cgaacagcgt gcgcagcgcg gtcaccggcg accgggtgca cgacaccggt tgctcgctca
      601 aactgtttcg ccgcccggtg gtggaaaaaa tgcagctctt cgagggcatg caccgattct
      661 ttcccgccct cgccttgatg catgggttca ccgtcaccga ggtgcctgtg cgccactatc
      721 cacgcacccg gggcacgtcg aaatacggcg tgggcaaccg cctctttaaa ggcctgtacg
      781 acttggtggc cgtgcgatgg atgcagcacc gttgcttgcg ctaccaatac cgtgccgccg
      841 cgacgacatc atcgccttcg gcctcggccc ccacacgact atgacactcg acaccatctg
      901 gctgattatc ggattcctgg ggcaggggat cttctttatg cgctgggtcg tccaatggat
      961 cgcctccgag cgtcatgcgg aaagtcgcgt gccgaccgcc ttctggtaca tgagtatgat
     1021 cggcgggctg attacgctgg cctatgcgat ctatcggcag gatccggtgt tcatcgccgg
     1081 gcagagcatc ggcagcatcg tctatctccg caatctcatg ctgattcatc gtcccaacca
     1141 agccgactcc ggcacggcat ccccggcgac caaatcctaa tggaacttca catgaacgga
     1201 cagcacccgt cctcgcagac gccggcgcag gccgaccgat cgatccagcc gctggcgctc
     1261 atcctcttgc tggcactggc cgccgtgctc ttcttcgttg gactcggctc cctcggactg
     1321 accgatcgag atgaaggacg gaacgccgaa gccggccgcg aaatgtacga aacgggcaat
     1381 tacatcagcc ccaccttcaa ttatgagccg cgcttcgcca aaccggtctt tgtctattgg
     1441 ctgatgagtc tctcctatca cgtcttcggc gtgagcgaat tcgccgcacg attcccgtcg
     1501 gccatcttcg gtgtcggatt gatcctgctc caatacctct ttctgacacg ctgccggggg
     1561 ccagtagtgg gcctcttcgg cgcggccatg ctgttactga acctggaaat catcggcctc
     1621 agccggatgg cgctcaccga cagcgtgttg atcttcttca caaccttgtc gctctacgga
     1681 ttctggctgg gtttgtacgg agaaggccgt gaacgccact acctgtggtt cttctacatc
     1741 ggcatggcgc tggcgaccct gacgaagggc ccgatcggct tcctgattcc catgctggcg
     1801 gtagggttgc acctgtggct gacccgctcg tgggggctct tttggcgtca tggatttccc
     1861 attcccggcc tcctgctgtt cctgctcctg gcgttgccct ggtacctcat catgctgaat
     1921 atccatggac agcgatatac gacctcggcg cagggcgaca cgatcggccg gttcttcggc
     1981 accatggaag ggcacggcgg cacgctggtc ttttatctcc ccgtgttcct gcttggattt
     2041 tttccctgga gcggcctgct gcctttcgcc tggtaccagg cctatcgcag ctggcgggat
     2101 tcgaaacgat ccggcgcctt gcctccgtcg cagacctcgg tgctggatgt ccacccttcg
     2161 ccgcatgcgc tcgaatggtt caccgccgcc tgggtcctgg gcggattggt ctttttcagc
     2221 ctgtcctcga cccgcttgcc gcattacatc ggtcctctgt ttcctgcggc ggcgatcttg
     2281 acggcgtcct actggaaccg ctgcgtgacc gaccaggcca ctccagggct gcgcgctgcg
     2341 atccacacca tcaccgccgt aggctgcatc ctggcgctgg cctttgcttc attgccgccc
     2401 ctctatgcca agttcgcagg aaagctgatc gatgaatttc ccctggcggg gcaggtgacg
     2461 ctcggaccgg gcccctatac cgtcgcctcc atctttctgg tgggcatggg cctcatcgcc
     2521 tacttcggat tcagcgaaac gagacgaccg gccgtgttct gggtcgccgg cggatcgctg
     2581 gctctcgtcg tcctcgcgac cacacagttg atcttcccgc tggtgcatca ctttgtgatc
     2641 gaaccgcccc agcaactcgc cgaggtcgca ggcgtcaatc tcgggcccaa cgatcggttg
     2701 atcctctacg ggcagccacg cccctccttg gtgttctatg caaagcggaa agccatcgtc
     2761 gtgccgaaga atgaagaagc gaacatcaaa ccctatctga ctcaaccagg gcgcacgatg
     2821 attctcctgc cggcggcgtt tcgaaacagg cttccgtttg aaaccatgga ctacccggta
     2881 ttactcgagc gctacggcta catcctgctg gccaaccaat cgctgatcca tgtgccggaa
     2941 gaggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngacgt cctgccattc
     3061 gcaacgccat acctccggcc accagcatgg ctccggatgc aagcaggatg tgatccgtca
     3121 tcggatcaac gatgcgatgc gtcagcaccc agaagagacc tgtcagcgat aggactaccg
     3181 gagtgatacg gataagcgcc tgcccgcagg accgtcccca ccagcgcaac gggccattaa
     3241 agatcgaccc caccacaaac cccagcacca tccccgccac cacgtccccc gggaaatggg
     3301 agccccgcca gacccggctg gctccgaccc acgcagccag gacaaacggt acccatcgat
     3361 accggggcag cgccctcgcc agcacggtcg ccaccgcgac ggtcgctgag gtatgaccgg
     3421 acggaaacga atccagaccg gaatccagcg atggccacca ttgccaccct cccgaatgtg
     3481 tgagccttgg cctgggacga ccgatgatgt gcttcagccc gttcaccagt atcgccaccg
     3541 caccgtgcgc caagaggcta tccagcgcca cgcgtgtgag tgcctggcgc ttcagcatgt
     3601 acccggcccc cagaagcacc aggctgatgg tcacgagagt gccgccattg cccagcttct
     3661 cgcccagatc accaagcgat tggagcgccg atagattgtg cgagcggagg aaccacagga
     3721 tggggatatc gatataaaac agcgccgcaa acgacccaaa caagacaatc accgatagca
     3781 ggaccgccgg gatcgatggg caggccggtg gtggctcagg ctgcgccggt tgcgacgccg
     3841 cctgcccgtc acctggttcg atcatggaac ccaatcggcg agaaacacat tgaactctcc
     3901 gggctctttc gcatgccggt ccgacaccca gacgagggtc ttcccgtccg gcgagaacat
     3961 gggaaagctg ttgaactgtc cctcgaacgt taaccgttcc tgcccggaac cgtcctcgtt
     4021 aacgagatag aggtgaaact ccggtcgccc cgatgcgttc cgcgtgtcta cattcgacgc
     4081 aaaaatcacc cgatggccgt cgggatggaa aaagggcgaa aaatttgaag cgccgttatt
     4141 cgtcacctgc tgcttattcg acccgtcggc attcatgata aaaatttcaa gtcgcccggg
     4201 ctccacgaga ttttgagcga gcagcgcctt gtactgatcc agctcttcct gattgctcgg
     4261 atgctgcgcg cgatacacga tccgcttgct gtcgggcgag aagaacgccc cgccgtcgta
     4321 tccaatctcc tgcgtcagcc gacgggggtg ggtcccatcc agatccatcg cgtagatatc
     4381 cagatccccg tcacgcatag aggtccagac gatcgtcttg ccgttgggag agaccgtggc
     4441 ctcggcgtcg taccccggcg tgttcgtcaa acgttgcggg tccagcccgt cgatccggac
     4501 ggagaagaga tcgtagtcat ccaacgccca gcgataggcc cctccgtcac gcttcggctt
     4561 cggaggacag ttgggccctc gcaggtgggt ggacgagtat aagactcggc gatcgccggg
     4621 gaaaaaatac ccgcaggtcg tggtgccggc accggtgctg accatccgaa tcttttcgct
     4681 cccgagatcc atcacgtaca tttgataaca gccgagcggg atgtcggacg gatgcatagc
     4741 cgcggcaaac gtgtctttca tccaattgtt cgtcgactgg aagatgagtt tattcccgct
     4801 gaaagaaaaa taggcctcgg cattctgtcg gccgaatgtc agctgccgga tattcgtcag
     4861 atgccgctcg gccgaggcgg acgcattcgc cggtgcctgc gcagacttcg gctcggctgc
     4921 tcccgccaaa accgttgaag tggcgaccag cgcgagaccc accattccga tcatcaccgt
     4981 cttaacgtgt ttcaattcgc acctctgtat tcatcctatc ctcccaatct ccccgtgcaa
     5041 ccacggcgcc ttcctgaaag accacataac tctgccaccc gtaaaaaaac aagagccggg
     5101 ccacacgtcc cagcgcctgg ggcgtcaccc cgtacagcag cgtcaccacg ctgccgggat
     5161 gctcctcacg ccgacaggat accagcagcg ccatggcggc gccttcatac atcttacccg
     5221 ccacggaaaa tccgccgtct aggaaacgca cacggtcacc acacgaccgc acggccgctg
     5281 cggctatcgg attctcgcgc ggatctccca gcaccagcaa cgatcccgac ggtacggatc
     5341 ggttgccctc acctggttga agcacgatcg tgcgcactgc atcaggcttc gccgattcct
     5401 gtgcgacgat acgctggagg atatcgccga acgggccagg ctgcgcgggg gagccttggg
     5461 caaggacgac gcttcgctgt ccatccgtca catacagatt cagcacggga gccatctcgc
     5521 tccgggccac acgacgaaac agatgatagt ccggatccag ccggactgcc gacggcggct
     5581 ccgcaagggg cacaaccaca tcctcctgtg cggcgctcaa ctgaacacgg acgcgatggg
     5641 tgcgctcccc gctcaacaca aactccaact cgaccggggc ccgatagggc tcatcgctct
     5701 gggcgatatg gatcgccgcc tcgaccgcat tcgcgtggcc gaaagcgccg gagaccgtct
     5761 gcaggcgaag gccggacagg gcgagttccg gcacaccggc acgctcgacc cactgggcaa
     5821 aaaaccatcg gaggtcgcga cccgcctctt cctgaaaggt tcgctccaga tcactccaat
     5881 cagctaccgt cccaaggtac tgttccggga tgcgcttcag cgcccgccaa aatgccgcat
     5941 cgcccacttc ctgtctcagc gcatgaaaga ccatggcgga tttctgatag ccgatggcat
     6001 tgtctttctc atcgctcttt tgcgcaaacc tcctgagcgg gtacccttcg ccgggacgca
     6061 catagacgga atagcccaac agcatcagcc gccgttgttc acgagcctga gcctcgtcgc
     6121 cggtcagttc gtgatagtag taattcgaca ggtaggtcgt cagcccttcc acccagttgc
     6181 cccgactcac ccggttgaac accccgttgc cgatccagga atgcacgatc tcatgcccca
     6241 gggcgtacgg ttgcgtatag tggcgcttga tgaccccgct ccccagcagc gtaaaggacg
     6301 gcatgcccaa tccgctcgag aaaaagtttt cgacgacggc aaattgtgaa aagggatagg
     6361 cccccagcaa cgggatatag gcgtcgagat atctggctga tgcgtcgagg tactcgtcgg
     6421 cgagcgcggc atcgtccggg aacagatacg tagccaattg aatcgcctgt ccggattgcg
     6481 ccttccaggt acgggtcttg accacaaagc ggttggccac caccgtcagc gcttcgctct
     6541 tcgtagacac aatccatcgc gaggcgtcag cctgggccgt gccctgcgtg acggcagccc
     6601 acccttcagg cagcgtgatg cgtacatcat aggtggccag cgaatcgtcg aggtccgggt
     6661 accactgact ctcgctgctc agatacaccc cctcaggacc gatgtgaccg gctgtttcgc
     6721 tcggcgtgac aaaccgcagg tggcgtggat cccggggagg atcatcaatc accccgtgat
     6781 agacaaactt cagccgtatc gatccgccgg cctggttggc aggcggctgc ttcaggagaa
     6841 tgcgctgcga ttccgcctga cttgacgacc tgtcccgtgc aaatggaacg gagggggcga
     6901 actcacaggg gtcctgacaa tgcgtgagat cctcgacccg ttccaacaag agcgtcggtg
     6961 caagggaaaa tgccagctcc tgtccgggaa atcctcccgc aacggtcact cgatccgtcg
     7021 cgatcagtcg atgggaatca ggagacaact cgagcgcgag ctgatggtgt tcaatcgtcg
     7081 gagggggagc aacagaatca gcgactgcgg gcgcaggaag gagacaaatt gtgaggagac
     7141 tgaaacggat gaggcgatac aaaccattca cccttcaccg gcaccctgag gtggacggtg
     7201 aagattaaca ctcccgacag accatcacaa gctggatagc catcaacgcc cgctccaaat
     7261 caatgggctt gtccaggacc tcttgcgggc ccaacgccca ggcctcggcc agcaagggat
     7321 cgttttgatg tccgcccaaa atgatcgtcc tgccggcata gtctcgcgcc gtgagcgtcc
     7381 gcaagacctc cacgccgttc atctccggca tagcgagatc caaaatcaga agatccggcg
     7441 ccgactcacc gatcagtcgc aacgcttcac gaccatcgca cgccgcgcga acgtgatacc
     7501 cgcgcaggct aagaaatctc accagaagat cgcgcgccat cacgtcgtca tcgacgacaa
     7561 ggatctgctc atcgggaagc tgatctatct ctcccccggc atgagactgc tcggatgaag
     7621 cctgtcgcct cgcctgctgc gccacacgat ggacggcccc gatgagcaca tccagcgaca
     7681 gcccctttcg caggaaatcc gtgacgcgca actcgcgcgc ctgattctcc agttcctccg
     7741 tcgcgccgcc acccagcata atcaccccgg cacgggggtc atgcgttcgg atctccttga
     7801 gcaccgccag accgtccatt tccggcatgc gcaaatcgag caaggtcacc agcgggcgaa
     7861 gctgttgaaa taagacgaca ccctcacacc cgctcgtcgc gatgctgact tgatacccgt
     7921 gacgggccag cgccatctgc aacaagtcgc agtgcaaccg atcatcgtcg atcaccagta
     7981 acgtgctcat catcttatct cgtttgtgcg tcacaaccag tcgcgcaagg ccgcagacgg
     8041 tagcagcccg ttagcaggag cctagcatgg gcacgacaag atgagaagaa attgaatgaa
     8101 aacgtctgga gtggatcacg gaccgccttc cgtcaggaaa ggccagtccg cgtgcttcaa
     8161 gagagttcgt gacgaacccg tcacgaatca tgcgggctag aacaacgcgt cgacaaacgc
     8221 cttggcgtca aagtcctgga ggtcatcgac ggcttccccg acgccgatta agcggaccgg
     8281 gatcttgaag gtgtcggcaa tggccaccac gatgccacca cgcgccgttc catccagctt
     8341 ggtcagggcg attccagtca ccccgaccgc ctcgtggaac tgtctcgcct gggcaatggc
     8401 attctggccg accgtcgcat ccaacaccaa cagcacctca tgtggcgccc cctggcactc
     8461 ttgcgcgacc acccgcttga tcttccgcaa ttcgtccatg agattcgttt tcgtgtgcag
     8521 ccgcccggcg gtatcgatca acaccacgtc cgacccgcgc gctttcgcag cggtcatccc
     8581 gtcgtaggcg accgcagccg gatccgcacc cggtcgatgg cggatcacat cgacctcgat
     8641 gcggtccgcc cagacctgca attgatcgat cgccgccgcg cggaaggtat ccccggccac
     8701 gagtaatggt tttttcccct gttgtcgaaa ccgttgcgtc agcttcgcca ccgtcgtggt
     8761 cttgccgaca ccgttgaccc ccacggcaag aatcacaaag ggacgcggcc cctgtgccac
     8821 caattgctcc atcgaggcag attgtgtcgg aagaagaata tccagcacgg attgccgcag
     8881 caggtctcgt actttatcgg ccgacgagaa attaccgccc cgcatctggg cccgcagctg
     8941 ccccatcaca cgttccacga ccggcatgcc cagatctgcg ctgatcaagg ccacctccag
     9001 ctcatccagc agcgccggat cagccgcccg ccccaggagt cgatccagtt gtcccgtgac
     9061 tgcatcacgt gttttggaga gccccgcact tagtttctga agccaatcca ccgcatacct
     9121 caccctctct tcgaccgact cgaatgattg atgaacgatc cagactagga cttctcgggc
     9181 acggcgatca aattctggat catacctgat ccgatccgcg tgacaccaat agccgtggaa
     9241 ccggagcaac ggcgcggagc acggcctttt cgcgccggct catgcgttgc gcctccgcat
     9301 cactacgctc gtggtcatat ccgcacaaat ggagaatgcc gtgaatcagg agtaccgcta
     9361 actcgtggtc aacgccctgt tcatgaccac acgcctgacg aatggcctgc ggcagggaaa
     9421 taacgacgtc cccgagaaga gacgagggtg gcccgggccc ttccctggtc gcaaacgcca
     9481 gcacatccgt cgtcttgtca cgatgacgaa aggttcgatt caggcgccgc atcctgacat
     9541 cgccgacgat ctcaagactc aacagcgttt cggcttcgcc gactgcttgc aagacatgca
     9601 tggcgagttg attgagggcg ccgaggcgaa ggggccaccg cgtcagccgc atgccgatca
     9661 caaccggcat taatgagact ggccccagga acctgtggaa ggagccgcgg gacgggtggg
     9721 cttagaatcc gaacgatggc ccttcgtctg agcctcacct ttccgtggct gagccggagg
     9781 cgcaggactg gccgtatgac gatcgtaggc cttgatgatc tcctggacaa ggcgatgccg
     9841 cacgacatct cgctcatcga agtagacgaa ctcgatcccg gcaatgtcct gaagaatgtc
     9901 gcgcacctcg atgaggccgg acacccggtc cgacggcagg tcgatctgcg taatgtcccc
     9961 cgtcacgacg gccttcgaat ggaagcctaa ccgtgtgagg aacatcttca tctgttcggc
    10021 agtcgcgttt tgcgcttcgt ccaggatcac aaatgaatcg ttcagggtac ggccgcgcat
    10081 gaacgcaagg ggcgcaattt cgatatcccc tcgctcaatc aaacgattgg cccgttccat
    10141 atccatcata tcgaacaggg catcgtagag cggccgaagg tagggattga ccttggcata
    10201 catgtcgccg ggcaaatacc ccagcttctc gccggcctcc acggccggcc ttgcgaggat
    10261 gatgcggctg acatcttttt tcatcagcgc actgaccgcc atcgccatgg ccaaataggt
    10321 cttgcccgtg cccgcaggac cgataccgat cacgatgtcg tgtttttcga tcgcgtccag
    10381 atagtatttc tgagtcggag tcttcggaac gatgaatcgc ttccgagtga cgatggggga
    10441 agcgctgaaa agcagttctt tgagagaagc gtcgtggttg tggcggagag cggtcagcgc
    10501 atgcgtcaca tcatcggacc gcaggtcata cccttcattc gtcaacgaag cgagctcatt
    10561 gagaacccgt tccgcctgct tgacggtctc aggcgtaccc tccaccgtca cttcttcacc
    10621 ccgcgccgag catcgcacac cgaactcttc ctcaatcagc ttgaggtgtc gatcatggtg
    10681 cccgaacagg gctgcggtat tcgtgccttc ccgtagtttg atcttgcgca cgccggtgtc
    10741 gaaaactcct ttcgtcagga cgatagggag attctaacaa accgggaaag tccggaacaa
    10801 ggtcggaggg gacagaacag aaaataagga ggcccaccgg aaccgactag ccttgagtgg
    10861 ggaggaccgt cagcgattgg gcagtagagg gtatcgagag ctcaaaatcc tgccaggccg
    10921 tgccaccctg cccatcttcc accatgatct tgatgcgatg agttcccccg acacccggcg
    10981 tcactttcca ggtcacgtgg cccgtcgcct tgtcgatcgt catccctgga ggccctgtct
    11041 cgagcccata gctgatgcta tcgccatcga catccttcgc ttggacgacg tactcgtatt
    11101 gctctctatt ggtcaacgcg gccggacttg agaggatctg tggcggtgaa ttaccgagga
    11161 cgatcggcac tgatcgagca ggtgtggccg tcgcatccag atcccttgcg acgacttcga
    11221 ccgccacaat atcctttcgg ccaaacccgg ttgtatcaag aacgttttcn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tccgtggatc aatcgcaaca ttggccccga
    11401 tgtttgagct cggaagacct ccgggagacc ctccaccgaa cccgcaggtt gcacagtcac
    11461 cgaagtttcc ctgactcata ttgatcagga ggccgaacgg actgccgccg tcccgtcctg
    11521 agcccatatg gaccacgata ccgaacgcca atccttctcc aagagagccg aacttattga
    11581 ccgtttgatt cgctcctgag gtagtcgttc cgatgatcgg ctcttgatat gcactccccg
    11641 ttttatagaa gagtgcccag agtcggctcg ttccggatga cacgcagatg tcgttcgttg
    11701 gaataaaggt ggggaaaaac accacccctc cgaataccgt tgggctactc acagcacgtt
    11761 cgccgatgcc gtaagggact gggttagtcg gtgggttggc gggttcgacc agtttagtca
    11821 cccaaccatc tttactctga accaatccga tcaaaccggt aaacgtcgtg gcaccggaca
    11881 cgccggagac ttggttggtt ccactggcct gaccacaatc gccgactccc accacacaga
    11941 cggtggcatt cgagacatcc acaagatcat tgtccatgca gttgagcgcg ccggctgatt
    12001 gattgcatcc ggaattgagg accgagtctt tcaaccccac gaagtattgc gtggacacgt
    12061 cggtcttatc ggatttgctg taataccgcc cgctgcccgc aaagacccac acgttagccg
    12121 catcgtctgt ggtcacggaa ggcgcggtcg caacggggcc catctccgcc cctgatacga
    12181 acgtatccag catctccgtg ggcacgcgag cacttcccga ggcgatgccc cactgagtcg
    12241 gactggtgac cgtaccgaac ttggccgtag ctccccctgc tcccatggtc aaacgataca
    12301 tcttgccccg ccagggaagc gctccgtcat gaatcactcg cccgaaatac acgacatcgt
    12361 gacggtaatc gagatctcga tcgaaagccg tgatgtctcc gatatacgaa ttccaggacc
    12421 ccaccgaata cgacgtcacg aggctgttgc cgctacccgg gcccgtcgca aggtcgacga
    12481 caaagacttt tgcactttga actacgctgg cgtcatatcc ggtcgggcca gacccgacca
    12541 ccatgtacca cttcgcgttc gtattgctgg ccttggcatc ggccgcggga ttcacgcgaa
    12601 ccacggtagg agcactggtc gtcaacccca aactcgaact gctgaacgac cagagcaatt
    12661 tgggagcatc gggattcgtg atatccatcg caaagtacga actgtagaag gtgtagtccg
    12721 tgccgctgat attgaccgtc atcggcggcg caccggttcc cgccgaacag gcgccgcaac
    12781 ttccccccat tctgaatccg ccgatcagga tcgtgcccca cccgttcgga tgatcggtgt
    12841 ctggagtgaa gatgcggaca tccgccactt tgagcggcaa atcaacataa tacgtatgct
    12901 ggtagtcggc acggctcaaa aactccagct gcggaaggag ttcatagggt acaaatcccc
    12961 acaattcatc ccctaatgga gccccggtgg aattgtcagc aggggcagtc gtaaaccagc
    13021 cgtgttcagt cgtattggcg ggagcagagg cactcgcatc atcgccggga tggtaataac
    13081 ccgcgttgaa cgcgtgcagc atgccgtcgt ttccccccac gtaggcaacc tgtctgcggt
    13141 tgtaccactt ctggagaaat gccgaataac tctgatcccc atagatggca tcatgccggt
    13201 ccttcggacc cgcaaccacc gtcggagtgg agtgaatgac atccccgagt ttccacacct
    13261 tcaggctccc gctcccggcc ggaacttgaa gacggcgatc cctcgtcgtc gcctgttccg
    13321 cgcatgtccc cacatcgcac ccacgcacaa aattaatgat cttggtccct tccgcactgg
    13381 acgcggcacg aaggtatggt cggagcgtcg gctcgttggc tgtcgtgaat gccagctgtt
    13441 cgccggaatc aaccacgccg tcatgatcgg aatcgaccca tgtcaggatg gtgcgggtgg
    13501 tggagtcttt caacgccagt tgcttcccgg cttcccaaat cggaagaata tcgctcaatg
    13561 cctgcccgca cggattgcaa tccgccgtcg tcaccacgcc atcgctattc tgatcgttcg
    13621 gcaatccatc gccatccgaa tctacatatt tgtcgacctt caccgaattc gacgcggagt
    13681 caaaacgggt cttgatgatt ttgtcgactt tgtagtcgag acgaccgtcc tgattcgtgt
    13741 cctctcgggt attgccgaac gtgtcaatga acagggcctg cgtatagccg gtccatttca
    13801 catcgctcag cgtcgagggc tcgatcgtgc tgggaaagaa atacgattga tacaaggccc
    13861 cttcaccggt cgaggcagta gccaggacgg ataccgatga accggacgcg cttcgcctga
    13921 gaatgcttgc gattgtggcg agcagcttgt cctgaagatc gtcaacgttg gaggactcga
    13981 aataggcgtc cgggataccg tccggcgtcg ccgctcccgt gaggttgttt tccttatccc
    14041 attcattcgt ttgagggacg ccgtcattgt tcgaatcctc gaacccgccc aaccgggccg
    14101 cttgggccag gattccgcga ccattgatgt tgccgaacgc aaagaaggag tacaccgtca
    14161 ggctctgagt ccctgcgatg caatgcccgg tgactgttaa acctgcgatg gtgccgtcag
    14221 tcgtgccgct acaaggccgg aggtcggtcg tgtgggccca ataggcgacg tcatccaagt
    14281 agtggctgcc gctatcagca taactcgttt tgtgctctgc caacagatca ctgaaaggag
    14341 tggctgcgtg tgtattgcag gtgccatcga tggtacgcgg aggagctgcg ccatcgctcc
    14401 cggtgcaatg ctgcccatga tgggcatgcg catagtcttg cagagccgtc gggatattct
    14461 ggtcttgtgt cggttcgccg tccgtaaaga cgatcacaaa cgtccgacag caggccacaa
    14521 cctgagaact ggcggaccat ggcggggtat ggttttgtcc aaaaaaatac gggtctcggc
    14581 cacaggcact cgtgatatat ccggcggggc acgtttcagt accgaccaat gccgttattt
    14641 cacttgttcc gatcgaaccg gcaccgctcg tggcaaaatt gacgccgttt gaaatgccac
    14701 cggaaaaggc gatcgggtag acatagcccg tcgcaaaagt ggattggatc tgcgcgacat
    14761 accgtgcggt ttcatataac gcctcactca acggagtcca tgtcgaagga tacgactcct
    14821 gcaccgcatc aaccatggcc gcggtgtttg tcgtgaacgt ctcaacgtcg ccgtccgccc
    14881 agtcgatcga ctggcgcgat ccagctccca ccagcatacg cgcgccttcg ctgctggact
    14941 taaactcaac cagcccgaac cgcgccttgc tgccgatctg ctgaatgact ccggtaggtt
    15001 cagatgagta cccgactttc aattcatact tccgaacgct gtatgagtca ccgcagttgc
    15061 tattgaacgt cgtgtcattg tccacacaga agtagggcgc agccggtgtt ccgcttgccg
    15121 aaagaccaat cgagattgtt gctggatcgc tgcctctatc ggccaatgga atacggccga
    15181 cataggtatt agctccggtc ccgccgccat aattgacgtc tgcgcgttca ttactcagac
    15241 ccgcaccctg cgcccgaaca gtctttagag ccggagtgcc gctcgtcggg caggtgccgt
    15301 cggcagctct cgtgctggta caatctcctc cgatcatcgc tcgcttcacc gcatcgaacc
    15361 ggcgcaacgt cgcccagttc aagaaattcc cgtcccattc cgtgtccgga caggctgtcg
    15421 ccaatgcggt cttgccggtt ccgctctcga atcgagtatc ggttgtgttg taggtatagc
    15481 aacgcagcga atcaaagtac ccggagtacc gtgtagtatt ggtccatgtg gtcgtagtcg
    15541 acgtgctccc atccgccaac gtcccacacg acgacgattc acaggctcga ttgctcatac
    15601 tgcccgagtt gtccatcacc acaatgatat tcggcgtcgt cgcgttcgac acgaaagccg
    15661 gcacagccgt gtaatcggcg ttggacacga cggcgacggc atcacgggag ccaagcaacg
    15721 cagcactcag gctgccggcc accaacatca cagcgacaaa ggttcggcga ttcatgcgtt
    15781 cttcctcctt atctcaagca ggacaacagc cgtcgattct cggctacaag ttgagacaag
    15841 tcctttcatg cgctattgcg gcaacgtgac caccatcttg tcgatgtggc cctctttcag
    15901 atgaaacttg acgagcgaag tgggaatgat ccgctcgatc tcgagcggtt ccccctcgtg
    15961 attgacgacc accaccgcgg acttcaagac ataactacga ccatcgatgc gaatgcttgc
    16021 tccttgcacc acgtcgatca cgccggactg aaacccgacg gtcgaaaaca gcatcgcctc
    16081 cgggccttgc gctgtctccg cgagactgat agacggcacg gaaggcatcg tctgtccgag
    16141 tgtcagcagg ccgagcacca caataaacgc tgtgttgttc atcgccatgc tccctctctc
    16201 agttgatttt cttgacacag gtatcgccgt ccaaacagcc gtacaccgag gtcaccgtgc
    16261 tattggaacc ggtcgcgggg ttactcgcca tgcaggtaat ccgatagaca tgttctgtgc
    16321 cggccactcc tgtgccctgc ccactcttcg tatgcgtata caagagatcg atgtcgccgt
    16381 tgactgtgaa tccgggaaga ttcgtcatca caaaattcgg tgcggtgctc gccacatccg
    16441 catagttgac agccggtgta tttgccggag ccggcgacgg cagatcgtag ccataaattt
    16501 ctgcgtacaa tgtggtggca ttggccgtcg gaaccggccc cgtcggcgcg atgaacgaag
    16561 ccgggagcac gcctggaggg gacaacgtct gctgaatgat gttcgcggcc agtccttcac
    16621 acgaatcggc agccgctacc acagcctcgg tcgtgcggaa aaatcccgcc attcgattct
    16681 ccatgctggt caccgtgatg gaggcgatgc cgagtatggt gagaatcaac aacatcaaca
    16741 tgacggtgag caatgcgatg ccctgctgat tgttcaatcc ggctcctccg ccctcgcgac
    16801 ccgcgccgtg catgccctgt tgcagattca tcacgtcgtc tctcgttaca ggtttctcgg
    16861 ttgaatcgtg cggatcatca cgcgccggcg ctgttgcata taagtactcg cgttataacc
    16921 cgcatccgca gatggattat ggtcgccgac gatcacaggc ccggtggtat tgacggcctt
    16981 ggttcccttc tcatctagac cgtcatccgc attcgtcggc ctcaccacca ggctcacttg
    17041 cgccagcctg atcttatccg gcgtccacgg ggcaatcgcc cacgcactgt tggagacaaa
    17101 atctccctgc gtgaaggtgg gaaatcccga cgtcgaggag gcatcttgat catcgacaac
    17161 cccgtccggg aataagggat tggggacggc ttgattgcag ccgtcacagg cataggcgag
    17221 ttgcaagtct tccacgccat cgaccagcgg cacgttgttt cgcacgaggc aaggcgtgtc
    17281 actgccgcag gtcgccggcg tattcgccac cacttgatac cgcacgcatt gcatcaaata
    17341 cacaggcgtg ccgaccggga attgtccatc gacgaagttt ccggtaccga agccaatcga
    17401 agtggcattg acagcctgca ccgtctttga caatgcaccg ccgatggaaa tcgtcgatcc
    17461 gaccacaaga ccttgtgcaa ccatctccgt cataacggct ccggaaagac tgatgctgtt
    17521 gtcatacgtc ttattgttcg gcgttccacc gaccgccgcc gtcagcgtcc accctgtcgt
    17581 attcatcacc ggcagcacca tcgacacgct atcggtcccg gtgtccgcac ctgtcggcgt
    17641 ttggtcttgg ggcagcagtc caaccggctt gacgacggcg ccgatggtgg cattgcacgc
    17701 gcccacagat ccggttgccg gatcggtcgc attgtaatta aagccggcca gtttgatatc
    17761 ccggctgagc aagtcgatgg ccaatcgaac attctgttgc gtgtctgcga cctgtgtgtt
    17821 gacgacattg gcgcgattcg aagtgaccac cgtggtcatc gtcgccgcca caatcaccac
    17881 cgtgatcagg acagctgcca tcagttccac gagagtgaag ccctgctggt tccagtcgaa
    17941 tctacgcatc gtcatgctcc aggctacgcc tattccggcg cgaccaccgt cgtaaatatc
    18001 gcggtcttgg tccgagccgt gctcacatcc gtctgtctgg tttgccagct gacggtcacc
    18061 gtgacaagga accggttcat cgtcaccgga ttcgcggtgg ggtccggatc aaggcgggcc
    18121 gcggtgacaa gcccagtggc acccgtgaga ccggaattcg ccacgagcgc ctgccattgg
    18181 gtgcaatctc ccaacgccat gcgttgagtg ttcgcgctct gcgcgcaggc cacgctcgtg
    18241 ttggtgttat ggtagtccaa aacacgctgc cggttatttt gaatgcgctc cgccatatcg
    18301 gcggcgatat tggtcacgcg cgacatgtcg ttggcatcga cattcttacc cagcgacatc
    18361 cccgagagac ccgctaatcc caacagtccg accgagagca caccggccgc aatcatggct
    18421 tccaacagcg taaacccccg ctgtgcggcg gcgagcagac tgaagcaggg gtgtgagttt
    18481 tgacggtgtt tcattgggcc tctatgggca cgtcgcatgc gcacaccagt tcaccttgcc
    18541 tgccggagtg acgaccacgg aataggtcgt cccctgttgg gacgtcagcg ccacaggaac
    18601 attcgccgca gccccgctca acccttgctg ggtgaattga atcgtcggac ctcccgtgaa
    18661 tccgacgacg gcctggggca acacaatagg ggctaacgca cctccgaaat cgacattcac
    18721 cctgcccgac acaaggacca atgtggctgt gacggcaaga ttccgattca tcgctgccat
    18781 ccgggccagg ttgagactgc cagccaattc cgttgtggct tgtttcagct gatagcgggc
    18841 attccattga agatagttgg ggacggccac catggccaga atcccgacaa tcgcgaccgc
    18901 tatcatcacc tcgataaggg tgaagcccct ctggcggtgg acagcagcag accgttggag
    18961 agagatagag acacacatgg caacagccag atgagcaagt gccatgccga tggatatgac
    19021 atgggatgat ggcctaacag attgatatat caagcccatt catactggac ggcgtacaat
    19081 tccgagcgtt ccgtgatcac ttcgatgaca atttttgtcg gatttctggc gtacttcacc
    19141 tactggtgag ccaggaatga tgagagggga agggcgcaag agaagccgcg accaacctct
    19201 tgctgcgggc agagaccgga aaaatgcacc caggataggc gccagatcaa acgggctgtt
    19261 tctgactttg gcccgcgcat aattaacaag caggagcggc cttgaacaga gagtatcgcc
    19321 ctgggcaaaa cgtgagacac ggcgaacggg agggcactat ttgatcgcaa ctgcccgcac
    19381 ctgtttatac gtggtgagtc cttgcagcac tttctcgata ccgtcttgca ccaacgtcct
    19441 catcccgtca ctctttgcga gtgtgagtag ttccccggtg cgcgccctgg cctgaatcag
    19501 tgccttcatg gaatcagaca ccaccatcaa ctcatggatg ggcactcgtc cacggtaccc
    19561 ggtctggtta cacgtgtcac accctcgtcc acgaaacagt gtgagccccg gattatatgc
    19621 aggatgcacg ccttcccaat caccctcccc aaaggcctgc acgagttcgt catattcgct
    19681 gcgcgtggga tggtaggcct cgcggcagtt gggacagatg cgcttgcaca accgcatcgc
    19741 caacactccc aacatggcat ctgaaaaatt gaacgggtcg caccccagat cgagcaatcg
    19801 caccacagtc tcgacggcgc tattcgtatg aatggtactg aacaccaagt gtccggtcag
    19861 agaggcttca atggcaatat ccgccgtctc cttgtcccgc atttccccga tcatgatcac
    19921 gtccggatct gctctcagga aggccctcat ggtcgtggca aaagtgagtc caatcttggg
    19981 atgaacctgc acctgccgca atccatcttg cgtaatctcg atcggatcct ccgccgtcca
    20041 gatctttcgc tcatcggtat taatcgacgc cagaatggca tgtaaggtgg tggtctttcc
    20101 tgatccggtc ggaccggcgc acaggacgat gccatgcggc ttttgagcca actgtcctac
    20161 gagccggcga gttccgtcgc tgaactccag atcctccaag cggcgagctc cgtgggcggt
    20221 cagcaggcga atcacgacat cttcattgaa gcccgaagtg ggaagtgttg ccaccctcaa
    20281 ttcgatctcc tgccccgtac ccaacttgaa ccggattttg ccatcctgcg gcttgcgccg
    20341 ctcggcaatg tccagactgg ccataatctt caggcgggag acgatcgccc ggcgataggc
    20401 cgccggaatc ttcatgtacg taaagcaggt gccgtcgacg cggaagcgca cagccgtttc
    20461 ctttcgatcg gcatagggct caatatggat atccgatgcc ccgcgccgat aggcgtcggt
    20521 aatgatctga ttggcgagcc ggacgatggc cgaatcgttt tccgtgattg ccgcaatcgt
    20581 cgcattttgt tgttcttcga gctgcgcttc actgaccagc tcgccaagaa tgtcggatat
    20641 cgcatcgccc gactcgcgcc cttgaatggc attcaacaac cgcgcaatat cgcaacggag
    20701 tccgacacga taggacagtg cctggcctgg aaacgctcgc cgcacatcga acagcttgtc
    20761 cggattgtgc gggtcgtcga tcaggacttc gattccgttg ccctgacgcc tgagcggaac
    20821 ccaatggttc atccgaagat aatccaagct gagattcttt aataactccc gctccatgac
    20881 cgtgcgctcg tcataggcga gaaacgggca gtgatagaat tctgccagtg cggcaccgag
    20941 cgccggcttc ggtatccgat accgctccat gagcacagta gccacatcga gccgctttcg
    21001 cgcggcttca gccgtggcaa gcactaactc ctcctgttgt agaaaaccct gttcgacaag
    21061 ccggtggtag caagcagcgg agcgcgtctg cgccaaaggg ctgaccgctt gggaacactc
    21121 actgactggt aacatggaca ccgtggcacg actcatggga ccgattcctt tctgaatacc
    21181 gtggagacga ttcgattgta gatgattgtc tgaactcacc atgctggcag agtgaccgcg
    21241 tcaacctcgt agtgctgccg tcaaattttg gcctgccagc ttccaggggc cacaaacacc
    21301 agcggcatgc cctgcaccag cccatcgcgg aggtcgtggt tgtaccaagc ttccaacatc
    21361 cccgacccgt tcgtgccgtc cacaatcgct ccttgcgcca ccaccccacc gtagactttg
    21421 agatgccctg cataccgcac gtcacccgcg acacacagaa caccctgcag atgaataccg
    21481 gacagttgaa caggaatcct ggctccaaga gattgctgac cttccggcgg tggactgaga
    21541 gctggaaccg gtttcccatg cgcccctgcc ttccagagca catgcgcatt cacaatgaag
    21601 atgccctcgg catactcttg atcaagcacg atcgtcccca aattatccgg tcgtggaggc
    21661 atcccatcaa gcgtgtcgac gaagatcaat ccacgatggt caccggggcc cttcgaggca
    21721 aacacttccg aggctgggtg ccccgctcct ggctgaatca caccgccggc atagagcaat
    21781 ccatcccggt ctatgccgta gtactgcccg aactgcctgg ccattcgctt caaggtgtcg
    21841 taatcccact ggtctacctt gagaccgggc accggatcct ggtgcgtgtg aagattcagt
    21901 ggaactcccg accaggcctc tgccggaagc tgcgcaaaaa aggcgtcgcc accgagtcgg
    21961 atggtgaacc agcgatcctc gcggtgcgtc atttcgtcat aggactgccc cgtcaccggc
    22021 gccaacccgc tcttcatcgg gaccgcctcc ggccgaggga aatacgcctt gccacgtacc
    22081 accatgtcgc cccaatgggc gaacacggaa ccggctgagg atggcgcgga ctcatttccc
    22141 actgtgccgc cctggatcgg cgcgtgcagc gccggaacgg cataggcgcc gaattcgact
    22201 gataccgttt tcgttactcg tgaggtatgg ccggatccgg ccgtcacctc caccgtgcag
    22261 agcagtcctg gccgcgtcgc cccatagact cgtaacttga gaatcctcgc aagccccttg
    22321 agggatttga accaacccgt ttgaggatcg ttcataagcc ggtcgtgctg agcatgggcc
    22381 gcatcgaaga ccacatccgg agacgcaccg gttcccgcaa actgtgcgcg cccctgtgca
    22441 tcgaaatagg aggcatctcc ctgtgcattc accagccgtt tcgtcaacca cgtcgtctcg
    22501 actccctgcg gatgcagcgc aggatcatga aaccatccca tcaccacatc aaccgccgct
    22561 tcagcggcgt ggtgcgcaag ccgttcctct tgtaacgcac tggcgccgac gatctcctgg
    22621 ccggccaact gcatagaagt catccccagc aaggtcagca gaaacaccac catgagcacc
    22681 gccaacaacg ccattcccct ctcatctcgc cgcaccgcaa ttcgtgatcg tcccatgctt
    22741 gtcttcatag ttattccttt tacctattac atccggattg ccacgtcccg caccagcctc
    22801 gatccttgtc gtccggcctg tatcgccaaa cgtacccgca ccacctcgct caagtccgtc
    22861 gtcacacgcc catccctatc aaaatattgc agatcgaact gactcacatc gcccaagagc
    22921 gtactcgcac cgccatccac atcacgcatg acccgcatcg ccccatcatc ctggcgcttc
    22981 acgtagtaac gcactcgatt cagcaggaat atggcgctac gcacaggaaa ctgttcggca
    23041 gtcggtgtca ccaatgtcag cgtctgcttc cgtccatcgg cagccaactg attccaggcg
    23101 caatgcacgg ctgtgcacac cagtagttgc ttgcctttcg gccaaccggc tccgtcttcg
    23161 acggggagat cctggcggcc gatttctgcg acttgtgtca gcgtagtcga cgcaccactc
    23221 aagttggcga agaattcgat ctcgtccgac ttcgccttga gaaacggcga gtcttcacct
    23281 agcaacccgg ctccagccag acgtacttcg ctgcacagca catccagtcc gaggcgcagg
    23341 tcctgattcg tcaccatcgt cgaatgctgt ccgctgaacc gcaagccgac tgaggaaaag
    23401 acatgaatcg aagcggatat tgcgatactg ctgatcgcca ttgcgatgag gagttctatg
    23461 agactcgctc cggcagagcc gaggcaccac ccaatattca tgcgcttcgg cgcgttcgaa
    23521 ccgttgacca tgccgcgcct catgacaaca cagacaggcc gacataccga ggattggccc
    23581 tgatcgtgcg aacgcgaacc tctttctcct gccctcccat agtccggaag attgcacgcg
    23641 cctcgatagt cgccaggctc gccccggccg gctcgtggcc gcgattcagt tccacggtcc
    23701 agacaagccg gatgttggac tgggtggcac tggccgtgaa gatgccgtcg ccgttcgtct
    23761 gatcatcctg caagccatcg tcgtgcatga cggactccac tattccgtct tggtccacgt
    23821 cgtccatcaa caaccgctcc cacgccacac tccgcttcgc ctccaggcgt gattcagcga
    23881 gcgccagcgc ggttgtcgtc aacgccccgc gctgcagacc gcgctcagcc cattcacaca
    23941 tgccgatcgt tccgatcacg ccgatcgaca gcaccaccaa cgccaccatg ctctccacga
    24001 gggtgaaccc tctggcactc atcaacgcag atcgtcgcgt cacaagattc ggctcacatt
    24061 gggtcatgac agggtcaccc gtccggtgat gctcaccgtg acttgatgtg tcgcacgacg
    24121 accgtcgacc aggaccatgg tggtcgccgt ggccgaacga ccgctgggct gaaacatgat
    24181 ttccggtttc gtcgtcatcg actcaaccgc cgtgcctttg tgagcgaact cataccggcg
    24241 cagcaagctt gtgtcacagt ccatgcattc cgtgcgcaat tcagactgtt ccgcatccac
    24301 gaccactcga atacgctcat gcctcgccat cgccaattgc cgggccatgc gcaactccga
    24361 tgcgatctcc accatcaccg cgcgctgttg atgttttgcg acaagggcgg cccatccagg
    24421 cacactcacg cccaccacaa tcccgatgat cgcgactacc gtacacagct ccacaagact
    24481 cccgccacgt tcgttcatac cggctccttt cttcgagcgc gtagagcatt tccaagttcc
    24541 atgccgtttc tcattcccga cgcctgtaga agcaatgagg ccgcctcttc tacgaagcgg
    24601 ccggcgaagc tatagaaatg ttgaggaaat ctgccgtgag ccgtggaatg ttacctgagg
    24661 taggacagaa aaaagcgcgt gaacagttat gaactgttcc gaatcggata cagtcagtca
    24721 tccgttcaga tacagttcgg cgaggcgctg atcctttgaa attggctctg ggtaacgatt
    24781 tgaaggcagt tactgaggaa tgtcgatcag cgcccaatac cactcaagca ggggctgatg
    24841 aaacaacagg gcaaggattg atcccactgc caagaacggt ccgaatggaa tgtactgctc
    24901 acgccgcatg accccgatgg cgatgagccc gacaccgaca atggacccca gaaaagatcc
    24961 gatcataatc gccaacaaca ccggctgcca gccaaggaag gccccgacca tggccatcag
    25021 cttgatatcc ccgcccccca tcccttcctt cccgaaaaca tagggactga tccaagctaa
    25081 aaaccataag atcccgccgc ccaacaacac accgagcaag gaatcgacaa gtccgatcgg
    25141 gaggatcaag atggcacaca gcagaccgat gacgattccc ggcagcgtaa cggcgtccgg
    25201 gatcatcgta tgggaaaggt cggtccccgt caccacgata agcgcagacg ccagcgccgc
    25261 ataggcccag gccgccggcg caaacccaaa cgtccaaaag accagcacgt atccgatcgc
    25321 gttggccgct tccaccactg ggtactgaat cgcaattggc gcatggcagg cccggcagcg
    25381 gccccgcaga agcagataac tcacaatcgg aatgttgtcg tagaaagcaa tggcctggcg
    25441 acaggaggga cagtgagagg ccggccaggc aaccgactct tcacgcggca ggcggtagat
    25501 acagacattc aaaaaactgc caatcaccgc gccgaatagc agtaccgtaa cgtatgaaat
    25561 catgaacatt cctgaaacaa tgtggccagg ctcgacatga ccccctccta agtatacgta
    25621 ttcaatccta gggcaataag agcaggcgcg aggacccctc gtcttggctc tttccattta
    25681 caaacgccgg gggcactgcg tataatccgg gagcaatttt ggcgcggaag atcgcaaacc
    25741 tcaatcagtg aattgccttt acctgaagtg gagctagatg tacatgtcgc cagtcgccaa
    25801 gaccacgaaa agtcgagcca aagtatccgc accggatccg gtccttcccc ctcgcaaacg
    25861 acgtcccgaa gccctcacaa cccgcgagca ggaaatcctt gagttgatct ggaccggatt
    25921 caagaacaaa gaaattgctc agcggttaaa gatcagcgtc aagacagtcg aggcgcatcg
    25981 ggcgaatatg atgaaaaaga tccgggtctc caataccgcc caactgctca aggccgccat
    26041 ccaagggaaa atgctgaagc tccggtgagc ccttcgcatc cttccttacg gcttctttcc
    26101 cgcaccttcc cgacgttgac gcaatgcctc atacagcacg atggctgctg atgccgagac
    26161 attgagcgag ctgactttcc ccatcatggg aatgtgggcg gcctcatcgc aggcgtcacg
    26221 cacccctggg cgaacccccg tcccttcccc gccgaagacg agcgctaccg ggccgctgaa
    26281 atccagcgcg gtatagggct tcggagcctc agcatccaac gcatagaccc agatgcctgc
    26341 gccttgaaga cgttcgatca agcgcgacag attcggcacg cgcccgacgg gcatgtaatc
    26401 gacagccccg gcagaggcct ttgccacgac actcgtcagc cccactgcgc ggcgctccgg
    26461 aataaagacg ccttgcacac ccgacgcctc ggccgttcgc agaatcgccc cgaggttgtg
    26521 cgggtcttcc acaccgtcca acaggaccag cagtggcgtc tgcccttcag cctgtgcgga
    26581 agcgagaata tcgtccggct ccgcataggc tttggcagcg accacaccca ccaccccctg
    26641 gtggtgaccg gaaggaacca ggcgatccaa tgcagcccgc ggttccactc gcacgggcac
    26701 acgctgatcc ctggccagac gcgcgatttc cccgaactgt cggtcggttc ccaacacgag
    26761 gagacgttgg atcggacgaa ccccggcgcg aagcgcttcg cgcacggcgt ggaggccgta
    26821 gatcacctcc agcgagttat cgtttccatc gactggtgcc atccggccgg tcctcaatcg
    26881 taatgccatg ggaggccaac tctgctcgta actggtcggc gagttggtaa ttcttcgatc
    26941 gctttgcggc gagacgtgcg gctagcttgt cggcaatgtc ctcgtctgac agggtgtccg
    27001 tggatgcctc cgctccgccg gatgatggct gctgccgctg acttttaaat cgccaggtgt
    27061 cggtctggag gagtcccagg acgatcccta aggcgcggaa ctcttgccgc accacccggc
    27121 gcatctcacc ggacaatccg acctcgagag ccttgttcgc ttccccacgt aacgtctgta
    27181 aggcggccag cgcattgggc gtattcagat catcgtccat ggcggccacg aatgccgctc
    27241 gcgcccgcat cgtggactcc tgcatctgct gatcggccgc accatgggct gaggccgatt
    27301 cattcagccg ttcaaagaga tcgtaaaatc cgttgagtgc gtttttcgct tcgttcagac
    27361 tctgatcgga aaaatccaag gggcttcgat agtgcgtaga aaggaggaaa tagcgcagca
    27421 tctcgccggt caccgtgtcg gaccattccg acttctgaaa gatttcgcga atagtaaaaa
    27481 agttgcccag cgacttggac atcttctctt gattgatctg cacgaaccca ttgtgcaccc
    27541 agtaccgcgc gaactccttg cccgtggccc cgcaggattg cgcgatctca ttttcgtggt
    27601 gggggaaaat gagatccatc ccgccgccat ggatatcgaa ggtttcacca agatgccgca
    27661 tggccatagc ggaacattcg atatgccagc cgggacgacc cgggccccag gggctatccc
    27721 aggaaggctc accgggcttg ctgcctttcc acagcgcgaa atccatcggg tgccgtttcc
    27781 gctcatcgac atccacccgt gctcctgcct gcaaatcatc gacgttccgc ttcgagagcc
    27841 ggccgtacac cggataccga tcgacctgga aatagacatc cccgtcgaca cgatacgcca
    27901 ttcccttggc gagcaacgtg tcgacaagcc tcacaatgtc ggcgatatgt tccgtcgctc
    27961 tgggttcgag cgtcgcgcgg cgcacaccga gcttctccat atcctcgtaa taggcgttga
    28021 tgtaggtggt ggtgacgtgc tcgcagctca cgccctgttc attcgcccgc ttgatgatct
    28081 tgtcatcgac atccgtgaag ttcttggcaa actcgacgac gaaacctgaa tattccagat
    28141 accggcgcaa cacgtcgaac accagcgcac tccgcgcatg tccgatgtga caatagtcgt
    28201 acaccgtcac cccgcacaca tacatgcgga ccttgccggg caccaagggt tcgaacggct
    28261 ctttgcttcc ggtcagggta ttgtggacac gcaacataac gcgcaattac tccatagacg
    28321 gacgacgctt cggccagtgg gaccagagaa aatacccgag gcccacggca agacacaacc
    28381 cgatcaccag atccgcgtga tggaaatact gacgtaattg attccagtgc tcccccatct
    28441 tcaagcccac ataggccaac gcataacaga agggaagcgc tccgacaaat gaatagagca
    28501 cgaacttctt gacgtccatg cgcgcaatcc ccgccgggag agagatgaac gttcgaacta
    28561 cgggcagtag acggccgatc aacaccgccg cctctccatg tttcgcaaac caccgatcgg
    28621 ccagttccat atcatgctgc gacaccagaa catatcggcc atagcgctcg acaaacggtc
    28681 gccctcccca cacacccacg tagtaggcca ccaacgaccc cagcacgttc ccgatggcgc
    28741 cggcaagggt cacgcccatc atcgagaatt ggccggtgga gaccaggtag ccggaaaacg
    28801 gcatgatgat ttcactcggc agcgggatac aggcgctttc gatcgccatc gtaatgacaa
    28861 tacccgtata gccgaacgtc gaaatcgtgg cgatcacgaa ccggccgagc acttctatca
    28921 gccactcgat caatccggtc acgcctgggc tctccttcga ggatgcggct tcaccgtttt
    28981 tggacgcgcg gatggtcgcg agagcggttg ctcccacgcc ttgaactgtt ccagcacctc
    29041 atgatgggtc gtgtcgagat gaatcggggt caccgacacc atgcgctgct ccagcgcttc
    29101 gtggtcggca tcgttctcgc gactccacga ctgacgcgtc ccggcgatcc agtaatactt
    29161 ccgccccctc ggatcgactt tttcaacgat ggggttgttg aaacgacgcc gactcaaaca
    29221 ggtcactttc acacccttga tcgaccggac cggaacattc ggaatattca cgttcaggat
    29281 cgtttccggc ggcaatccat gccgcaatac ctcagcagcc actcgagccg catactgcgc
    29341 tccgacatcg aatcgaaatg tttcgttgcc ttcctgagac acggctatcg aaggaatgcc
    29401 taggatcgtt ccttccagag cccccgacac ggtgccggaa tacatcacat cgtcgccgag
    29461 attgacccct cgattaatcc ccgacacaat caacgacggc cgccctggca ataccttcac
    29521 cagagccaag ttgacacagt ctacgggcgt cccgttcacc ataaatacac ggggtgccat
    29581 cttggtaaga cgaaggggct tatgtaacgt gaccgcatgc ccgactgccg tgcgctcccg
    29641 gtcgggtgcg acaatccaca cctcccccaa tgcacccaac gccgccgcga ccgcgtgaat
    29701 cccgggagac gtaattccat cgtcattggt cacaaggatc cgtacacgcg ccacaaccgc
    29761 tcctttatgc ttcgcacacg tcgtgaaagg cgagcaccga aaccgctgga tcaagagtgg
    29821 ggacgaaaga ggcaggaacg cactcgaaca agctcacaag atcgtccgca atgatcaacg
    29881 tccctcagga cactgacagt ggtagagaac atcgtgccga tggtcggggc ggcgggattt
    29941 gaacccacga cccctcgcac cccaagcgag tgcgctaccg ggctgcgcta cgccccgaca
    30001 cacacgacat cgatgtaccc acaattggac gcagccgccc gcatcacaag aacagttcag
    30061 gacgaatggg aatcgatcat tctgatgatc gcctggagat cgcccctgac acgttgtgcc
    30121 agttctttcc gcgagctcag cttcttcacg gtaactcgtc ctcgacgagc ccagtagcgc
    30181 ttaaccccgg ccagcgtatg cccctcgtca tagagcatcc gcttgatctc cagtaccgta
    30241 tctacatctt tctgcacata gagccgctga ttcccacggc tcttcttcgg tctcaggaag
    30301 ctaaactccg attcccaaaa ccgcaagaca taagccggca gtttggaaat cttgctgact
    30361 tcgccgattt tgtaaaaaac tttacttccc agtcggggct cattccccat gaccggtcct
    30421 catgctgctt gtgctcgtaa caaggtagcg actaggaatt gacgtacttt ttaaacacct
    30481 gactgggacg aaacgtgacc actcgacggg gagtaatccc gatttcttct ccggttcgcg
    30541 ggttccgtcc cttgcgagcc cctttgcttc gtactacaaa attcccgaat cctgcaattt
    30601 tgaccgcgtc acctttttgc agcactccct tcagcagatt gagcacgagt tcgacgatat
    30661 cggctgcatc gttcttggag actccaacct gcttgaagat ttcgttcgcg atatccgcct
    30721 ttctcatgcc tttccccccg ccatccacta gagtcgttcc ttaggaagtg cttcttggtg
    30781 tcgagcgtcc aagacaaaga atttccccat aagttacgtg aggttacctg ccctgtcaag
    30841 gattatgtgc gcccgacgga ggtgaaaaga gcctagcgat ccgccagcag caatttatac
    30901 tcaatgctgt ccgccaacgc ctgccaactc gcttccataa tgttttccga cacaccgacg
    30961 gtgccccact tgtccttatg gtcgcccgac tcgatcagga cgcgcacctt tgattccgtc
    31021 cctttatccg cagagagcac acggacctta tagtcgagga gcttcacttc ccgaagttgc
    31081 ggatagaatt tttccaaggc tttgcggagc gcatggtcga gggcattcac aggaccggcc
    31141 ccgacggcgg ccgtgtgctc gacgacctcc ccgaccttca ccatcacggt ggcttccgac
    31201 agcagcagac cgtgctcctg cttcttctcg acgatcaccc ggaaaccaag cagctgaaag
    31261 gacgggcgat ggctgcccat cgctttgcgc atcagcaact caaacgatcc ttccgcgcct
    31321 tcgaattgat acccttggct ttcacgctcc ttgagcgtct cgaccagttc ctgcaacttt
    31381 gcgtgattct tgttcagtga aatgccgtac gcttcaacct tttccaggag cccgctgcgc
    31441 cccgcataat ccgacaccaa gatccgacga cggtttccga ccgctgcggg atcgatgtgt
    31501 tcgtaggtcg ccgcattctt gagcaccgca tgaatatgca cgccgccctt gtgcgcaaac
    31561 gccgcgtcac cgacatacgg ttgccgctta ttgggcatca ggttggcgat ctccgtcacg
    31621 aacccggata catccttcag gtgcgtcagc ttattgccga gcgcaggccg ctgcattttc
    31681 aactgtagat tcgggatgat ggagcagaga ttggcattgc cgcatcgctc accgatgccg
    31741 ttgatcgtcc cctgcacctg cagaatgccc gactccacgg cgacgagcga gttcgccacc
    31801 gccatttcgc tgtcgttgtg cgcatgaatc cccaatggca cgggacattc ctgcttcaca
    31861 atgcggcaaa tttcacggat ttcccacggc atggtcccgc cgttggtatc acagagaatg
    31921 acccgctcgg ctcctccggc aatcgctctg cgaatcgtct gcaacgcata gtcgggattg
    31981 gccttatacc cgtcgaaaaa atgctcggca tcgtaaaaca cccggcgatc cttggaccgc
    32041 aggtagtgaa tcgagtcttc gatcaactcc aaattcttgg cgagcgcaat gcccagtgca
    32101 tccgtcactt gaaacgacca actcttgccg aagagcgtaa tggtcggagt tcctgacgcg
    32161 agcagttctt gaagattttt atcttttcga accgagttgc tggctttgcg cgtcgacccg
    32221 aacgccacga tggtcgcatt cttaaaggga atggtcttga ccgtgcgaaa gaactcgatg
    32281 tctttcggat tcgcgccagg ccacccacct tcaatgaaat gcacaccgag ttcatccaac
    32341 cgttgcgcca cccggagttt atcctccacg gagaaactga catcctccgc ctgagcccca
    32401 tcccgcaagg tggtatcgta gatttcgagc gcggacgcct gcgccgccgt gagcgacggc
    32461 accgaaacag gttccgaacc gcgagggcgc cgcggcgctg tgttgcgacg tttcatagat
    32521 gtgagagtag cagggtcatg acggacctgt cgaggagacg gcacaccgac cgtgaccgcc
    32581 ctccgagcag actagcccag ggcaggcgcc gaaacccgat ccaggccgaa cgccgtgtga
    32641 agcgtacgca tggccaattc caaatacttt tcctcgatga cacaggaaat cttaatttcc
    32701 gacgtgctga tcatcatgat gttcacgcct tcgcgcgaga gcacctcgaa catctttgcc
    32761 gccactccgg aatgcgaccg catacccacc ccgatgagcg acaccttggc gatggattcc
    32821 gtcacagcca ccgaccgggc gccgatctct tcggacagac gctgaacgag atcgacggca
    32881 ttacgcaaat cagccttggg gaccgtgaac gaaatgtcgg tggtggaggc ctggctgacg
    32941 ttttggatga tcatatccac cacaatgttg gcattggcca cggccccgaa tacgcgcgcg
    33001 gcgatccctg gccgatccgg cacaccgaca atcgtaatct tggcctgatt gcggtccccc
    33061 gtcacacccg acaccatgac cccttccata tcggcatctt cacgtgtcac gagcgttccc
    33121 tttccttctt tgaaacttga attgacctcc accggcacgg agtacttggc cgcaaactcc
    33181 accgagcgac tctgcagcac cttggcgccg aggcttgcca tttccaacat ctcttcgtag
    33241 gagatcttat ccagacgccg cgcagccggc acgatgttcg ggtcggcagt gtagacaccg
    33301 tccacatccg tataaataat gcagcggtcg gccttgagcg ccgccgccag cgcaacggct
    33361 gtcagatcgg aacctccccg gcccagcgtc gtgacatccg agctggcgtt aatgccctgg
    33421 aatcccgcca caacggggat cacgcccgca gacaacgctt ccttgattcg atcagccgtg
    33481 acgcgggaaa ttctagcttt agtgtgcgcg ctgtcggtgt gaatgccgac ctgccggccc
    33541 gtgaaggacc gggcattcac ccctcgcccc cggagctcca tggcgagcaa cgcaatagtc
    33601 acgcgctccc ctgtcgagag cagcatgtcc aattcccgct cgtccggtgc accggtgacc
    33661 tcatgcgcca acttcagcag tcgatcggtt tccccgctca tggccgacaa caccactacc
    33721 acctggtggc cgtctttctg agcgcgttcg acacgttcgg cgacgcgatg aatccgttcg
    33781 accgtgccga cggatgttcc cccgtatttt tgaacgatca gcgccacagc tctatactcc
    33841 gcgcgggaat aatcgcagca tcaacttcgt cgcatcgcga ggcgcggtcc cggcgatgtt
    33901 cgcatcctgc tcgtgaaacc gcttggtgac tccggccgac ggcttggcgg cactatcaca
    33961 cagcgcatac aggatcggcg gaacctcagc accgtggctc tttgcgaggc cgggatcaca
    34021 cacgaccagc aaccgatagg ctggatgcgt cgccagtgcc gcgagaatcg gcccgacgac
    34081 ctgggcatcg aaccgctcaa tggcccgcac cttgtcctgc acatcagtcg cgtgcagcac
    34141 gtcgtcggaa agcccggcat gcagatagac gaagtccttc ttggccaact cctgcaccgc
    34201 cgcctgcacg caacccgtga actcattcgc gtcgcttccg tcggcaagcg gaagctcgac
    34261 cgcatccaac ccggcgcaca ccccaactcc acgatggaca tcactggatg acaccaccac
    34321 accggcaatc tggtatcgct ccggcaaggg cggccacacg gcggcacggc cctggcccca
    34381 caaccacagg caattggcgg gcttcaaccc ctcctgctca cgttcctcat tcaccggatg
    34441 gtcgcgcagg atgaaaaacg ccgcatccat gatcttgcgc agcacgtcgg cgccatcgcc
    34501 gctcggcaat gcatcggcaa tcgaccggcc gaccaacggt tgtggatcga gacacgtcgc
    34561 gcgggatttt cctccgaccc agaccatgag gtgtcgatgc ccggaaccgg ggtagaactg
    34621 aatcgattcc gagccaagct gctcattcac cgcttcgagc aactcacgag cctgctccgt
    34681 gtcgatgaga ccggcggtcg cgtcctccat gaggacatgc ggcccgagtt tttttatgtc
    34741 ggttgcccga tctttgctac cggaggcaga ctccccccgc accgtcacca tggtgcatcg
    34801 gaacaccacg tcctgctcac ccaccgtgac gccgagaccg gccgcctcca gcggaccggg
    34861 gcccggataa tactttttcg gatcatatcc cagcacagcc acactggtga catcacttcc
    34921 ggcggctccg gcttccgagg gaaggctcaa caaccccacc tcgccacgct gggcgatctg
    34981 gtcaaggttc ggagttgcgg ccgcttgaag cggggttttt ccaccgagtt ccggacacgc
    35041 gagatctgcc atcccgtcgg catgcaggat caaatatttc atgcaatgtt cctgaggtaa
    35101 tcagacatga aggaggcggg ccacatcatc gcgggtggct ttcaccgttt gaggctgacg
    35161 tgaaatgccg atcgccgtat cggcatcctt caaaccatgc ccggtcaacg tacacacaac
    35221 cgtggcacct tcttttaatt gtccggcctg actcaattta atgacaccgg ccacggaggt
    35281 agcggaggct ggttcgcaaa agacaccttc ctcactggcc accaacgcat aggcatgcag
    35341 aatttcttcg tcagtgacca tatcaatggc accggacgac tccttcaccg cagtgagagc
    35401 cgattgccaa ctggccgggt tgccgattcg gatcgccgtg gccaccgtct gtggctcttc
    35461 gacgatatgg cccaagacaa tcggcgccgc accagcggcc tgaaatccca tcatccgagg
    35521 caaccgcgtc gtctgattgg ccgcacgata ctcccgatag ccgttccaat aggcggtgat
    35581 attacctgca ttccctacag gaagtacatg catagccggt gcatcaccga gatcgtcaca
    35641 aatctccatc gccgccgttt tctgcccctc gatccggaag gggttcagag aattgaccaa
    35701 ctcaacgggg tacgtagccg acagctcttt gacgatggtc agcgcctggt caaaatttcc
    35761 ttcgacttga atgacggtgg cctgatgcat catcgcctgc gacaacttcc ccagcgcgat
    35821 cttgccggcc ggaatgagca catacacggc aatgcccgcc ctggccccgt acgccgcagc
    35881 ggaggcagac gtgtttccgg tggaggcgca aatgaccgcc cgcgcgcccc cctcgacagc
    35941 cttggagatc gccatggtca taccgcggtc tttgaaggat ccggtgggat tcatgccttc
    36001 atacttcaga tacagatcga tccccggagc gattttcttc gcgagccgcg ccgcacggat
    36061 cagtggcgta ttgccctcgc ccagtgtgat cacaggggtc tgcgccgtga ccggcaggaa
    36121 cttccgatat tcttcgatga tcccacgcca acgattcata acaatctatt cgaccatcct
    36181 ctcagcacaa ccggagagac acattcattc atcacgccct tcgactctga tcagcgtcgt
    36241 gggctcggag atgtaggaca tcggattgat ttcacggagc gcgttctgaa tatcccgctc
    36301 ttttgccata tgcgtcatga tcaccaccgg caccgtctgc ccctctttgc gtccctgctg
    36361 cagcaccgac gaaatgctga tcccataacg acccagcacc ccggcaatct gcgacaacac
    36421 gcccggccgg tcgaccacca taaagcgaat gtagtagagc gaggtaattt cttccatcgg
    36481 tcgcatgcgc aacggccgcc gctgatcggg ctggtaggat gcaggcggga cacgaccggc
    36541 ggcatccttg agcagattgc gcgcgatggc catgacatca ctcacgaccg cacttcccgt
    36601 cggcatcgac ccggcgcccc ggccatacag cacgacatct tcgacagcat cgcccactag
    36661 ctgaatcgca ttgtagacac cgtcgacctt cgcgatttga gaggccgacg gcaccatcgt
    36721 cggatgcacg cgcgcctcga tttcgccatc ggaatatttt gcgatcgcca gcagcttgat
    36781 tgtaaacccg aactccttcg cataagcaat atcgagcggc gtaagcccgg tgataccttc
    36841 ggtatagatc tctttgaaat tgacgggtgt cccgtaggcc aaactgacca tgatggtcag
    36901 cttgtgagcc gaatcaattc ccgccacatc gaaggtgggg tcagcctcgg cataaccggc
    36961 gcgtttcgcc tcttccagca ccacgtcgaa acgctggcct tcactggtca ttcggctaag
    37021 aatataattc gaggtgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc
    37141 cgtgaggact tgctggatat ccgtcgtgag cacgcccggc ggaatcgcaa tgccgcgatc
    37201 gcgcgtgatg tccaagtccg caatgcgaac cagcgtgacg ggtacaccca cccgccgacg
    37261 aatcaattca gcattctcga tcaacacacg agccacaccg gtgccgaccg ttccgaatcc
    37321 gattaacccg accccgatct cactcttcat gattcctggt catccaaatt caatactttg
    37381 cggatcccgc gcaaagcctg ccgggtgcga tgctcatttt ccaccagcgc aaaccgcacg
    37441 aattcgtctc cgccttctcc gaaaccgatt cccggcgata cggcgacctt ggcttcccgg
    37501 agaagcagct tcgaaaactc cagcgacccc atatgccgga acgggtccgg aatacgggcc
    37561 cacacgaaca tggtcgcgcg gggtaacgcc accggccagc cgatacgatt cagcccgttc
    37621 acgagaacat tgcgccggtt ttgataccgc tgcacgactt ccttgacgca gtcctgtggc
    37681 ccattcaacg cgacggtact ggcaatctga atcggctgaa aaattccgta gtcgagataa
    37741 ctcttcagct tggcgagcgc gccgaccact tcacggttgc cgacacagaa tccgactctc
    37801 cagccgggca tgttgtaggc cttggacagc gtataaaact ccacacccac atccttcgcc
    37861 tccggcactt gcagaaagct cggcgcctta tagccgtcga acgcgatatc ggcataggcc
    37921 aggtcgtgaa tcacgatcac gtcgtgttcc ttggcaaacg cgacgatctt cttgaaaaac
    37981 tcaagatcaa ccacggcggt cgtcgggttg tgcgggaaat tgatcacgag aatacgcgga
    38041 cgcggcaagg tttggcgata gacgcgcaac aggtcatcga aaaagtcgct gtcctggcgc
    38101 agctcaatcc cgcgcacttc cccgccggcg atgataaaac tgtacatatg gatgggatag
    38161 gtgggcgtgg gcgtcaacac cacatcgcct ggcccgatcg tagccagcgc caagtgggcg
    38221 atgccttcct tggagccgat cgtcacgatc gcttcggtct caggatccaa atccacatcg
    38281 taattgcgct tataccaacc ggtaatcgca tggcgcagct tcgtgatccc gcgtgaagct
    38341 gaataacgat ggtttttggc cttcctcgat gcttcgatca atttatcgac gatatgaggc
    38401 ggcgtgggct gatccgggtt gcccatgccg aagtcgatga tatcctcgcc ccgctgacgc
    38461 gcctcaagtt tcagggtttg tacttgcgca aatacgtagg ggggaagtcg tttaatgcgg
    38521 taaaagccgt cgccgatcgc catgaatttg tcagactcct acaggcgggt cctctgccga
    38581 acaggttcag tcgccatgcc tctgaaaggg gcgtatttta atggaggcct caaaacgagt
    38641 caatttcgct acgattgtca ggacttgggg gcgagcaccc tatcgtggtc acactcaaaa
    38701 cagtgaggcc tctcacgatc tgcctccttg ctcccccgta ccgtcttctg ttaataatgc
    38761 ctcagcaacc agagaattgt ctatgatttt cgaccagttc cgaaatcttt ctcctatgat
    38821 ctgcagggaa attgcggagg cgtaatggct cgactgggtg taaatatcga tcatgtggcg
    38881 acactccggc aggcgcgagg agggaccgat cccgacccct tgaccgcggc cattctcgtg
    38941 gaattggccg gcgccgatgg cctggttgtg catcttcggg aagaccgccg gcatattcaa
    39001 gatcgagatc tcaccatgtt gcgggaaatc gtgcgaacca agctggatct ggagatggcc
    39061 gccgacgacg ccatggccaa gatcgccctg agcgtcaaac cggacctggt gacactcgtc
    39121 ccggaacgcc gacaggagct gaccaccgaa ggcggtctcg atgtcgctaa ccaccgcgat
    39181 cgcattcaga aaatcgtcga tctgctccgc gacggaggca tccccacaag cttgtttgtc
    39241 gagccgagtc tcgatcaaat caaggcggcg cacaaaatcg gcgcggccta tgtcgagtta
    39301 cataccggcc gctactccaa cgccaagcgg tcgaaggaag aagacgagga gttcgaggcc
    39361 attacccagg cagccaaact ggcctacaag ctcggcctcg gcgtcaacgc cgggcatggc
    39421 ttgacctata aaaatgtgaa acgactcgcc aagttgcccg agattgtgga attcaacatc
    39481 ggtcacagca tcatcgcccg atccgtcatg gtcggattgg tccaggctgt gcgcgaaatg
    39541 agggaattgg tcgcctagtc cggccacccc cacggcacgg gctgtcaccc tcgctgcgag
    39601 aaaccgaccg ggagtcacag aacaccatga taccgatcgt gaccgccgag caaatgcgag
    39661 ccctggacca gcggacaatt acggaagcct tggtgccgtc cctgacgctg atggaacgag
    39721 ccggagcggg ggtcgtcact caccttgagg cccgatatgc accgcttgcc gggaaatcgg
    39781 tcatcatcct ctgcggcaag gggaacaatg gtggagacgg ttttgtcgtc ggacggctgt
    39841 tacgacgaaa acaggcccag gtccacatcc tgctgctcgc gtcccccgag gatctgagtc
    39901 gcgatgcaaa gacgatgtac cagcgatttc tccgatcagc agggaaatcg gccgttacat
    39961 gccccgccga cgtggagacg atccaacaac ggttggcgac cggggacatt ctcgtcgatg
    40021 cgattctcgg taccggcctc tccacccccg tcaccgggct ctatcgtgat gcgatcgaag
    40081 ccatcaccgc cgtcggccga ccgaccgtgg ccgtcgatct tccatcaggc ctgcatgccg
    40141 atacaggagc ggtgctggga gccgccgtgc gcgcggatct cacggtgacg ttgggattgc
    40201 cgaaggcggg gctctatagt ggatccggca tcgattgcgc tggaacggtt cgactcgtgg
    40261 atattgggat tcccgccgcc tttgtcgaag ccatcggaag tcggttgatg ctgctgaccg
    40321 atacggctgc tcgcgcggca ctccccccac gacgcccctc ggcacacaaa ggcacctacg
    40381 gccacctcgg cgtcatcgcc ggctcggtcg gcaagacagg cgcggcggcc atggcagcac
    40441 tctcagccct gcgcatcgga accggcttgg tgacggccgc aatcccctcg agcgccaatg
    40501 atattctgga agcaaaactc ctcgaagtga tgacactgcc gatgccggaa accaaagctc
    40561 ggaccttcgc gcgttccggt ttggatcgcc tcctcgcgtt cgccggcgcc cgggatgccg
    40621 tcgcgatcgg accgggcctg accacccatc ccgagaccgt cgacctggta caggaactcg
    40681 tgaagcgaat cgacaagccc tgcgtactcg atgccgatgc gttgaatgca ttggcgggga
    40741 aatcctcgtt actgaccgag tgcaagcgac cgccgattat cactcctcat ccaggcgaga
    40801 tggcaaggtt ggaaaccgag gcaaccacgc aatcggtcaa tgaagatcgt cttggcacgg
    40861 ccaccagatt tgcgcgggaa cgcggcgtct ttgtcatcct caaaggagcc cgcacggtga
    40921 ttgcgcgccc ggatggcctg gcagccatct gcccgaccgg caatcccgga atggccaccg
    40981 ccggtaccgg tgatgtcttg acgggcatgg tcggcgggct actcgcgcaa ggcttggccc
    41041 cttgggacgc cgcctgcgcg gcgacatact ttcatggttt ggccggagat ttggccgcgc
    41101 agcatctcgg ccaagccggc atgattgcga gtgatctcat tcagcacatc ccccatgccc
    41161 tcgcgccgac cacacacacc taagccggtg ccatcacctg cggcacctct caggcaaaca
    41221 accgcggggt ctccccctat cggcaaaccg tggcacattg tgcttcgatc tccgaaacac
    41281 acgcaccgcc tgggacgttg cctgggcaca ctcctccagg gaggcgaagt tctcgccctg
    41341 ttcggcgaac tgggagcagg caaaacctca ctggtcaaag gtatcgccga cgggctgctc
    41401 gccgaaccga cggacgtgag cagcccgacg tttacactca tccacgaata ccggggacgc
    41461 cttccattgg tccactccga cctctaccgc ctgaccgccg cccaactcaa agataccgga
    41521 ctcaacgact acttagacgg ccacactgtc actgcgatcg aatgggccga tcggtggggc
    41581 gacggtcttc cgtcggaccg gctcgatgtc cacttgtcgc accgcccccc ggcggcccgc
    41641 cgcgcaattc tcacagccag aggccctgct gcacggcgtc tgctggacgc gcttcgctcc
    41701 cggctctccg caaatcgccc cactagagcg gctcagcaaa cccgtgtaca attgaaccgg
    41761 ccaaggaggg cctcgcattg attcgattga tattggtggg aacgctgctg ctactcgcgc
    41821 tgtcgttttt tttgcagaac caggagcagg aagtcactct gcggtatttt ttcggtcttc
    41881 gatccgcctc aatcctgatc tataaaccga ttctggcggc cttcggcgtc ggactgttag
    41941 tgtccgggat tctgctgttt ccggcctggg tacgcggccg catcgaactc cggcgcaaaa
    42001 ccaaggcgct gcaggaggcc gaggtggatc tggagcgcct ccgacaagcc ctcgacaagg
    42061 ctgcccgccg cgtggggacg tcttcggaca ttcccgctga gggagagccc tccgatgggt
    42121 gacgccgacg gcaccccatt aatgcggcag tatcgcgaca tcaagcaggc ctatcgcgat
    42181 gccatccttt ttttccgtgt cggcgatttc tatgaaatgt tccaggagga cgcggtggag
    42241 gcgtccaaac ttctctccat tgcgctgaca tcacgcgata aatccagcga aaccccgatc
    42301 cctctctgcg gtgtccccta tcacgccgcc actaattaca tcgccaaact cctccgtgcg
    42361 ggacgaaccg tcgccctgtg cgagcaagtc gaagacccca aactggccaa gggtctggtg
    42421 cgcagggaag tcgtccggct ctataccccc ggcacactca tcgatagcga attccttccg
    42481 tccacggaat ccaacgtgct ggccgcggta tacgtgcgcc ttcgatccgc agggaccgga
    42541 cgcaaagagc cctcctatgg cctcgcaacg cttgaagtct cgaccggaga ctattggctc
    42601 agtgagtttc atgggatcca ggcagaagcc tccctgcggg acgaactggc gcggcttgaa
    42661 ccgaaagaat tgctgtttcc cgaagatctc tctccggacg aacagcaatg gaccgccgat
    42721 ctgaccggcc cccgatgctg cgcacaacct gcgacgtggt tcgacctgga gcagggtcgc
    42781 atgacgttac aagaacagtt tcgcgtgcat tccatggaag ccttcggctg ccaccgactc
    42841 acactgggca tccaagccgg ggcggcagtg ttgcggtatg tcagggagac tcaaccgtcg
    42901 gcccctctcg accatatccg cccgccacga gtgagacagg atcatgacgc catgcacctg
    42961 gacagcgtca ccatccgcaa tctggaactc gtcaggcctg ccggtcgtgc ggaggactcg
    43021 tcgaaccagt ccacctatac gttattgggc gttctggatc gcaccgtcac cgccatggga
    43081 agccggttgc tccgtgagtg gctcctgcgg ccattggtca acagtgcggc aatcgaggcc
    43141 cgactgacag cggttgacga actcaaacga cagatcgacg cacgagttcg cctccgaacg
    43201 gcattgcgaa ccgtccagga catttcccgt ctctgtagcc gcatgtccct cggagttgcc
    43261 aatccgcggg atgtcctcgc tctgaaaatt tccgtctctt cgcttcccgc aattcaggcc
    43321 gaattgtccg ggctcgattc tccactcatc gccgacctga tgtcgtcatg ggataacgcc
    43381 caggatctct acgagttgat cgaaggagcc atagagcagg aggcgcccgt atcgattcgc
    43441 gacggcggca tcctgaagag cggataccat cctgaagtcg atgaacttcg caaggcaagt
    43501 cgggagggca aaggctggat taccggcctc gaagccaaag aacgcgaacg gaccggcgtc
    43561 gagtcgctta aaatcagata taaccaagtc ttcgggtact atattgaact cacgaaggcg
    43621 aatctcggga aggtaccgcc cgactatatc cgcaagcaaa ccctggtcaa tgccgagcgc
    43681 ttcatgaccg ccgagttaaa ggaacttgaa gagcgggtca cgggagccga cacaaaactg
    43741 acggccctgg agcaagcgct gttcgaacag ctccggacac gactcgctga ggaaacagcc
    43801 cgccttcaag agatcagccg acgattggcc acccttgacg tggtagcctc cctcgcggaa
    43861 accgccgcgc tcaatcgcta tgtgcgacca accgtcgacg aaggtgatgg cctccatatt
    43921 gttcagggcc gacacccggt cgtcgaacgt cttgatctct ccggcggatt tatcccgaac
    43981 gacacccatc tggatctggc taccagccgg ctgcacattc tgactggccc caacatggct
    44041 gggaagagca cctacctccg acaggtcgct ctgattacct tgatggccca gatgggaagc
    44101 tttgtccccg cctcggaagc ccggatcggg ttgactgatc gcatatttac cagagtcggg
    44161 gcatccgaca atctcgcagg tggccagagt accttcatgg ttgagatgac ggaatcagcc
    44221 catatcctca actgcgccac acctcgcagc ttgatcctcc tcgatgaaat cggacgcggc
    44281 acgagcacct atgacgggct gagcattgcc tgggcaattg cagaatacat ccaggatcct
    44341 cagcgtctcg gcgcgcgcac gctcttcgcg actcactatc atgaaatgac gcaattggag
    44401 agtctacggg aagggattac gaactattgc gttgcggtac aggaacgtga cgggaaggtg
    44461 ctgtttctgc gaaagattat tcgagggggc gccgatcgca gctacggaat ccatgttgct
    44521 gaactggcag gtctccccga tcaggtaata caccgggcca aggccgtcct tgctcaactt
    44581 gagtcctcct cgtcatccgg gcggtcagtg caggagaacc agcagtcgct tcaatttgat
    44641 gccacgctcc ctgcgcctca tcctatgctt gaggaagttc gccaaatgaa tctcttctcc
    44701 atgacccccc tcgatgcgct caatcgactt gctgaactgc aacaacggct attacaggaa
    44761 tagctcacat cccggcctag acagttcctc cctcgacgtc gcaaataaaa aggcggcatc
    44821 cctttcggaa tgccgccttc ttacttcagg ccaatggcct tctactactc agtgatgtcc
    44881 accaccctga ttgtacttgg cagtccaggg aatcaggttg gcaagcggct tgccattggg
    44941 caacaacaca tcatattgca acggcaaatt cgctccatcc ggagtctttg gggacgtgtt
    45001 cagcaacgtc ttcgcggcgt ggcaaggagc atccacttcc gtcttccctt cacattcctt
    45061 caagcgaagg gaggagatgc tctttggctt acccatttcg ccggcattgt cggccaactt
    45121 cttgactgac gaaatcacgc ggcgattttg ctcgacttcc tgagccacga actctaccag
    45181 agtggtcggg gatccagggg gaacttcctt cgctgcagcc ggtccggaat gcggacgcgc
    45241 catggacttc agctgctccc acacattctt gtccgcggga tagaacttga ggttcttccc
    45301 tgggtgaatt ctctgccaaa ataaaccgct ctctgcgttg ccgccgaaca cagcgatgtt
    45361 gagccagacc gcttcatcgt atggatctag aatgatgaca cgcccctgca gcgtagtggg
    45421 cttgctcaga tctccccact cgaatcgctc ctggaacgcc tcgatggcca gcgcagtgga
    45481 ggcaaaaccc accaccaaag ccacggcagc cataaaggtc cacccctgct tttgaagctt
    45541 cataatcaca tcctccttaa agaagacaaa aaagtctcaa gaatgtcgcc tcgcgtataa
    45601 attctatttc aagaccttga atccagtaat ggtccgacca tcaagagtta cttccatcgc
    45661 cacaagctcc tgcgatgcct tgatgatttg atccatcaaa ctcttatcct taacagacaa
    45721 acgaacggtg gtttgcgcgt ggtgccagcc ggcgtaagga ttgttcttgt ccgtacctcc
    45781 ggtaaagccc cagccgcagc aggtaaacaa aggatctggc ggcttcacat ccatggatgt
    45841 tgaagaccga ccgccccctt caccggaagc cggatcaccg gtgttccagt actggatgcc
    45901 gaagagcaat tcggtatcag gattgtctgc ccattgcgac cacacccgac ccttcaaggt
    45961 attggtggtc tcacccttaa aaaactttcc gctgcccgtg gtgacttcgc tcggtccgcc
    46021 cccggctgga gcatccgccg ccaacgtaac accggcggtc aacaagccga caacggagga
    46081 gactgcaagt gcggctagaa ttccgaccct attcataacc ctaccctcct tggaaaaatg
    46141 atgaccagag aaccgttgaa tcacagtatc caaaaaaaga g
//
LOCUS       DAOQRM010000005        42170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_460_length_42170_cov_16.261308, whole genome shotgun sequence.
ACCESSION   DAOQRM010000005 DAOQRM010000000
VERSION     DAOQRM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 42170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42170
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_460_length_42170_cov_16.261308"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /gene="tatA"
                     /locus_tag="PKK81_01010"
     CDS             complement(<1..503)
                     /gene="tatA"
                     /locus_tag="PKK81_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNV31473.1"
                     /translation="MFGTMGFSELIIILVIVLIIFGAGKLPQIGEGVGKALKGFKKEV
                     NDIPPPENAPDQNETAAMPAQIQAQPAADIPQVAQPATVAPAAAPKATSPYTPGPELT
                     PGTTAALMAAAGPQGPQATQPTKPRVATVTPGQAAAGSAVAQSHQPPTMEERMAAPAP
                     VMRAQYPP"
     gene            complement(650..1867)
                     /locus_tag="PKK81_01015"
     CDS             complement(650..1867)
                     /locus_tag="PKK81_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNV31474.1"
                     /translation="MNVADRSHARVIRIQQAIREQPGLDGWLFYDFRHLDPIAYRVLL
                     LDPSLHVTRRWYYWVPAQGTPVKLQHRIEPHVLDGLPGDAHAYVSWRDQQAALGSLLH
                     PAKRIAMQYSPMNAIPYLSRVDAGTIDLVRSLGAEVVTSADLVQQFEAVWDDAQLASH
                     QVAAEGLRAIVDEAFGFVGTSLAARSSLTEYGLQQYILSRMQARGLVTSSPPIAAVNA
                     HSADPHYAPASEGSAPIRQGDLVLIDLWAKQPGPGAVYADITWTGFVGATVPARHQDI
                     FQIVRRARDAAVTFVQGRVRAGECPYGWEVDDVCRRVIQDAGYGQYFVHRTGHSIGEE
                     VHGNGANIDNLETQDARRLLPGTCFSIEPGIYLPDEFGIRSELDVYLSARDAVVYGQP
                     VQADLLPIPIPAS"
     gene            1962..2420
                     /locus_tag="PKK81_01020"
     CDS             1962..2420
                     /locus_tag="PKK81_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31475.1"
                     /translation="MAIVPSSDPAQYRVTFFFGPEAVEERPDHLRCVFNVKKRSWKGG
                     VQVAVDVARRHLDRARERIGYASWLAELIHSGHQEEREELTSRADDLFVQSLCTSALN
                     LALQAGIEQVNQQIDAATFADELHQLLDEQPEQVTDRIRLELDLGETSEI"
     gene            2554..3027
                     /locus_tag="PKK81_01025"
     CDS             2554..3027
                     /locus_tag="PKK81_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31476.1"
                     /translation="MNVSVYSPLKRRRSLWAQVLVFSLVTVCSLALLIAPVSAQESGG
                     ASNVPVSSNDASPSGVGIQAGSALATIVYFPLKLAFAIGGGVVGGLAYGFSGGSEQTA
                     KNIWIPSMYGTYIITPDHLKGDKPIRFLGVSGEPAREAGDYAPQEPAPLPLEPIR"
     gene            3029..3805
                     /locus_tag="PKK81_01030"
     CDS             3029..3805
                     /locus_tag="PKK81_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNV31477.1"
                     /translation="MKPVIGVTPDFNAGDRQEWGGKEPTYFLRARYVRAIEELGGVPV
                     ILPLVANRSARRRLLEGVDGLLLTGSGPDLDPTLYGEKKRYTFPVVAKRRSSFELDLV
                     RLAIRNHIPTLAICGGMQSMNVACGGSLYQDIPAQVDHVLQHRQTTPAVRLSHSISIT
                     PGSLLNRIVKRARMQVNSSHHQSVKAVGHALIASATAPDGIVEAIELPTHPFFLGLQW
                     HPEFLFERHLLHRRLFQTFLRAASRRPSLQPSPARRRNNS"
     gene            3869..5314
                     /locus_tag="PKK81_01035"
     CDS             3869..5314
                     /locus_tag="PKK81_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNV31478.1"
                     /translation="MGNPLFRTKSIERILADSDAPEHRLKRTLTAWDLTGLGIGAIIG
                     TGIFVLIGTAIVGDAHRPGAGPGIMLSFILSGITCALAALCYAEFAAMIPVAGSAYTY
                     SYATLGEFLAWLTGWNLILEYGVACVAVAIGWSGYFNNILKLCGLELPYWATHPPGAD
                     GGIANIPAAIIVLLVTCILIVGVKESARATCGIVLIKLAVIAFFIAVGTSSVDTANWS
                     PFMPFGFAGVGAAAAIVFFAYIGFDAVSTTAEEAKNPQRDLPIGIFASLAICTVLYIS
                     VAAILTGLVPYSQIDVHAPVAEGLRMVGFKWGAAIVATGAVAGITSVLVVMMLGQIRV
                     FFAMSRDGLLGPWLSGVHPKFRTPHHATYLTGVAVAIMAALIPIGEAADMTNIGTLFA
                     FVLVCIGIMVLRYTKPNHPRPFRMPFMPVVPILGVLACLGLMYFLPWMTWIRFFVWTI
                     IGVLVYATYGMRHSKLATRPSTEQSETPRPA"
     gene            5338..5553
                     /locus_tag="PKK81_01040"
     CDS             5338..5553
                     /locus_tag="PKK81_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31479.1"
                     /translation="MTGKTRTMLRLEQPGRLSEVLLQPGPQAFGQLPQVEEMLAPESR
                     YLLPQEQAEQPQAWEEQGASAEQAAPV"
     gene            complement(5892..6416)
                     /locus_tag="PKK81_01045"
     CDS             complement(5892..6416)
                     /locus_tag="PKK81_01045"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNV31480.1"
                     /translation="MTPPVTPPGPLEKSRRATMQWLLGFMDHLDRLGYVAAGFSLLAL
                     GMLIFVHAWYAFFLRSNDVPLLPAGLKLLNDLLLVIILLELFRTVVRFLQTEVLELEP
                     YLAVGIIACTRRVLTASAELSHQLEVVTREARQELFQQYVMDVGLNVTVIIVLILGVY
                     LLRMRPTRERPVTT"
     gene            complement(6413..6799)
                     /locus_tag="PKK81_01050"
     CDS             complement(6413..6799)
                     /locus_tag="PKK81_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31481.1"
                     /translation="MAIKKGDILDERKRFPDSCGLVVKVAGGGEGFQKILCCGHELTE
                     EDVVSDIISSRGRKKGALLPGAMLEEKRQFPDSCGLRLMVIDGGAGFQGIDCCGHTIT
                     AKAVNGLKFGQPLEDSPMPTGQAGTA"
     gene            complement(7022..8173)
                     /gene="nagZ"
                     /locus_tag="PKK81_01055"
     CDS             complement(7022..8173)
                     /gene="nagZ"
                     /locus_tag="PKK81_01055"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNV31482.1"
                     /translation="MTLREQIGQLFMIGFTGTTVTKDLASFLTASKPGGVIFFRRNLE
                     SVQQIVDLTNGLQKLSPAQPLLIAIDQEGGRVSRLPAEFTIFPPCGQLGQCNSSELAY
                     SAAATIAKELRAVGINMNMAPVLDVNSNPDNPVIGDRAFGAAPELVGEMGSATIGGLQ
                     DNMVVACGKHFPGHGDTATDSHKELPVVDAGLQRLRDTEFPPFQQAIRYGVASLMTAH
                     VLYRALDPEAPATLSSAVIQRLLREEFRYEGVVFTDDLEMHAIIDHDGIGEAAVRSFV
                     AGCDVLLICKDQDRVMTAMQAMERAVKDGRITQERLDQSLARVAKLKARYLHPYKPVT
                     ISDARLVVGCRSHKVLLDSWHKAYARIPKPTLVERGQAAPAHDSPVTHV"
     gene            complement(8245..9765)
                     /locus_tag="PKK81_01060"
     CDS             complement(8245..9765)
                     /locus_tag="PKK81_01060"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNV31483.1"
                     /translation="MKKIQVHAQVGRAENEAAEVLVLLHCEGGSDLTQEAAAIDAQLG
                     GQLAALIQRGEFEGKLGEGLLVHTQGKARAKRLLLAGLGKEKDLRVDAFRQALGIAIK
                     RVRQAKVTSFAVVLPAAILEEIPVQDVAQAMAEGAILGNYQFTAYRSPNGSKPIDVER
                     LTIYTSQASLLPQMTEGIRRGVATAEATVLVRDLCNHPANVMTPTRIVREAKAVAKES
                     GVRLKVLEQKDMEQLGMGALLGVARGSHEPPKFIILEYKGAKAKKGDPPVVLVGKTIT
                     FDTGGISLKPAENMEHMKADMTGGAEVLATMRAAARLKLPLHLVSILPVAENMPGGRA
                     MRPGDIVTTLSGKTVEVQNTDAEGRLILSDALAYATRYKPAVLIDIATLTGACVVALG
                     QFAIGMFGNNDQLKEHVRNAGQRAGERVWEMPLWEEYFEQLRSDVADMRNIGGRGGGM
                     ITAALFLSKFVGDCPWIHLDIASTDWSERERAYLPKGPTGIGTRLLIQFLLDRTLP"
     gene            complement(9847..10485)
                     /gene="queE"
                     /locus_tag="PKK81_01065"
     CDS             complement(9847..10485)
                     /gene="queE"
                     /locus_tag="PKK81_01065"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNV31484.1"
                     /translation="MVKITEIFHSIQGESTYAGRPCVFVRLTGCPLRCTWCDTAYAFY
                     GGRDMTIDDIVNQVRAFDCDLVEVTGGEPLSQTESLPLLSRLCDEGFEVLMETSGAID
                     TAGVDRRVHVILDVKCPGSGMTDRMYWPNLDRLAPHDEVKFVIKDRSDYEWARDVLHR
                     YDLPARCTVLMSPVFGEVEVRHLAEWILADKLPVRFQLQMHKYIWAPDMRGV"
     gene            complement(10537..11232)
                     /locus_tag="PKK81_01070"
     CDS             complement(10537..11232)
                     /locus_tag="PKK81_01070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV31485.1"
                     /translation="MTPLSLSHISGTSRQTDIVALFDVDNTLLPGQASEVRFFRFLWK
                     RGLVGWRELRDSVGWVLRNAPPVSLHPLRERKLYLAGKAAAEVESLAEEFCRAELFPR
                     LSAQGLARMDEHRRAGHHIVLVTGSLDFLIAPLATLLEVPTLLAAKLERQQRRFTGQV
                     CVPLPYGPGKRALIARLTQESRIDLTQSFAYGDSPGDVELLEMVGHPLVVNPIRGMDR
                     IARRNGWPVTRWK"
     gene            11457..11540
                     /locus_tag="PKK81_01075"
     tRNA            11457..11540
                     /locus_tag="PKK81_01075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11490..11492,aa:Leu,seq:gag)
     gene            11980..13578
                     /locus_tag="PKK81_01080"
     CDS             11980..13578
                     /locus_tag="PKK81_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV31486.1"
                     /translation="MTAQDVTTALPPPGDFSTVEALVRTLSGQASIGIFLSDPEGRTL
                     YLNERLRRIAGLPVVPTPGECWRNALAPEDHDPICSEWSGATNAERSFSREFRFRRPD
                     GSLRWVMAEAFPLRSGGGPSGGYVGIVRDITPRQLALDALHASEERYRTLAQQSPHAI
                     LVHADSAILFINEAGARLFGLATAHPIEGRPLWDFFPKEFLHDLPLAEAAPSPSVERQ
                     LVRPDGTVIDVELTAAPVMFDGHPAVQILGTDITERKVIAGQLHRAKKMATVATLAGG
                     IAHEFNNCLTAIMGFSDLALPSLVPDSRVHGHIQQVVLASKRARDLVTQMLMVGRQAD
                     GAKQPLSLDILLKETLRVLKMKLPESISLREWIPGATNPVLADPTQIHELCLNLLARS
                     QQAMTISGGVLEVRLDNMGLDASMTGHDLPLPPGQYVRLMISDTGDGIPADIRTPNMY
                     PLFAPPSTGSKAGAGLAELQRIVSEHGGTLRATSTAGQGTTIEVYLPAMLQPPQTVAQ
                     EPSRERTAPPTEAKEFLAEHDKER"
     gene            13687..14058
                     /locus_tag="PKK81_01085"
     CDS             13687..14058
                     /locus_tag="PKK81_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31487.1"
                     /translation="MPSVLIVDDEEAIRRLIRSTLEQAGYHVREAADGKAGLSHYRQS
                     PADLVIMDILMPDQDGLESILTLRREFPEARIMAITGGSDMIGILNFLDVARMLGARR
                     TLQKPFEMQQLLDAVQAEIAG"
     gene            14184..14621
                     /locus_tag="PKK81_01090"
     CDS             14184..14621
                     /locus_tag="PKK81_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31488.1"
                     /translation="MKTWWTPQLHAVFGGIVVAIGVFVMWEQAATTWALLVATASTAF
                     LWWQGRSIAAVWAWTTLGLGIESMTWPIVTMIRMRMGGSQPTEEQMETILNAVLFGLF
                     TSVFWITFAFGLFKRIREQNAPPAPPPGNGPVPARSKKKRARR"
     gene            complement(14628..17003)
                     /gene="priA"
                     /locus_tag="PKK81_01095"
     CDS             complement(14628..17003)
                     /gene="priA"
                     /locus_tag="PKK81_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNV31489.1"
                     /translation="MFDHRSFATPPVAPALPPRYADVVLPRRLHRPFTYVIPSSLKGQ
                     VAIGQSVIVPFGSQDLHGLVTAVYDRLPLGAPEQGLKAVRCLAPASPDHLLTSSQIDL
                     SRWVADRYAAPWGQCIKLVAPFVDQVDRRPSRYRPTAQGTDNSSLPEGLGEVETQVLS
                     RLRRRPKGITERTLAQGDKSRVMRAVQILIRRGLVVRCDDAVVPRAARAKKPPSPGAE
                     QAVFARLTVDDLPPPLEAAAWPDTVGRALSQDVYGSFLVQGARATRLWCLVQAARAAI
                     RRGRRVLVVTGDVENAGRLAEALAAAGERPLLLHSGLSARERAAVWQSAQDSSATILV
                     GTRMAVFAPIDRLGLVWVEGEDDASLKDEQSPRYHAREVARRRAFCDRALLVLASSHP
                     SLESWSAVQQGEMTACVYRDPSGFPRVQVIDLREYSRETPAGTLLSPPLYEGIQEALR
                     QNALAILYLNRKGFASVLHCGDCGAMPQCDACSVALTFFRRSNHVRCHYCGRTKPVPD
                     HCTRCQSLKLEPVGSGTERIEEAVRRKFPLARVGRVDGETIRRPADARAFSRLLAAGE
                     LDIVIGTQMLFRFGLQARAAFVGVPDADAGLHVPDFRSAERMYHGLMDAVELALPAHA
                     GGAVMIQTRLTDHHAIMAVAAGDDSVFLQQEQAFRQMLQYPPLTSLVRLDVSGTLEPV
                     VAQAAGRWAALLRAEVVSAGNRGTNAGDGSPLPQRSTGFGGETSSIVILGPSPAPHAM
                     ARGRYCWQILVKSLSLEVGKEIVVRTREVLDRESRRGGLRFDIDVDPVAMA"
     gene            complement(17169..18725)
                     /locus_tag="PKK81_01100"
     CDS             complement(17169..18725)
                     /locus_tag="PKK81_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV31490.1"
                     /translation="MRTLPMRASLIILSCSLAAVTAIPVHAQNAQSVRLGEAALTYAA
                     GSIRQEMPIDGVINVITGDNQLSGNRMMLGWSGTDTLYLKLKNPGDAALGELYTVYRR
                     SRKVFHPMTKQYMGYIINRVGVVKVIQIDAALVGVQVVRSYGPLSPGDPVMRFTPPLA
                     EEVVETASGHAEIEAMIVELQADKHMSLVSQGNLVYLDKGQDEGLRSGEYLEVFRTGG
                     GLPERKIGEVKILSTEPHTATAVLSKATARALIGDRVRSKHASPVEVMQYENGDSDVP
                     AATVQPVMNVRTDSAVAMPSESHSMSKPRVERAHGSTRINLDDLADQLEYESGEVKVK
                     PAGVPILEQLAEYLRTAAVSQQVRVEGHSDNMEIGPSLKGVFPTNWELSKARAAEIVR
                     YLIEKGGMDSAKLSAVGYGASRPVASNGTEEGRKKNRRIEVVLDSPEEGTQAQSAKAP
                     LSESDPIPAQFTYNQLDAAPASTAVVPPASATQAGSASVDAPIAPAPSDVAVPVSPVG
                     GETASPPPGS"
     gene            19294..19677
                     /gene="ndhC"
                     /locus_tag="PKK81_01105"
     CDS             19294..19677
                     /gene="ndhC"
                     /locus_tag="PKK81_01105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNV31491.1"
                     /translation="MAGSELLLEYLSRYFPILMFVFVALAFGAGTLLISYFVQPKYPE
                     PEKLSTYECGSEPFSDARMPFPVRYYIFAMLFVIFDVEVIFLYPWAIVFNKIGLIGLV
                     EMLIFIGLFLVAYVYAWRKGALEWD"
     gene            19668..20195
                     /locus_tag="PKK81_01110"
     CDS             19668..20195
                     /locus_tag="PKK81_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNV31492.1"
                     /translation="MGLIQLGGHDKDGTPDVITLTVEKAVNWARKGSLWPMTFGLACC
                     AIEMIAAVSSRYDMDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VIAMGSCATSGNIYDSYSVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQERIMQKR
                     VFLAQPKEVKDALKV"
     gene            20214..20711
                     /locus_tag="PKK81_01115"
     CDS             20214..20711
                     /locus_tag="PKK81_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNV31493.1"
                     /translation="MSQLAKRIEDTFPGACTQVVEWRGDVSVTVKREALHDIGKFLRD
                     DPAMRFDYIVHVSSVDWPDDEERFEVVYEVYSIRTRQRIRLKTRVPESDCIVDSLTDI
                     WKGADFMEREVYDMMGIRFRNHPDLRRILMPDEYEEGYPLRKDFPLRGKGWRDTFEFL
                     DEPTR"
     gene            20740..21987
                     /gene="nuoD"
                     /locus_tag="PKK81_01120"
     CDS             20740..21987
                     /gene="nuoD"
                     /locus_tag="PKK81_01120"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNV31494.1"
                     /translation="MAQFEDQRTTIYKVDPEHPEDATLPTLRTEELLLNMGPQHPSTH
                     GVLKVILELEGERLVKSTPVMGFLHRGVEKLAEEGTYHQFIPHTDRLDYVCAMYNNFA
                     YCRAVEKLMNITVPDRAEYLRTIVAEIQRIIGHQFWLGTQALDIGAMTVFFYCFRDRE
                     ILLDWFDELCGARLTTSWYRIGGVERDFTPSLFAKLKQFLDYFPPKIDEYVIFLEKNR
                     IWLARTKGVAVISAEDALSFGLSGPTLRGSGVDYDLRKYEPYSAYPKCEFSVPVGKNG
                     DTYDRYWIRVQEIYESVKIIRQCLEQIQDGPIMADVPSVTLPPKERVFTNLESMIQQF
                     KLFSQGFNAPPGEIYCGTEAHKGELGFYIVSTGGGKPYRLKIRAPSFIHMGAFDHMSK
                     GYMIADAVTIFGTYDIVMGECDR"
     gene            22068..24776
                     /locus_tag="PKK81_01125"
     CDS             22068..24776
                     /locus_tag="PKK81_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV31495.1"
                     /translation="MGLKPATNPDVEAATIELSIDGRTVSAKDGVSLYDVIASTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKAAAGMVIRTDGDDLFAARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKFIPEDTRSLVLDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGGFNQVVAKGDQPLACEFCGDCLAVCPVGAITNKY
                     SKYLYKPWQMKKTTTTCNYCGDGCQMHLETKDTEVVRVTSPLSWKNKWGDRTETAKGH
                     GGLCVRGRFGFQYIDSEQRLRQPLVRTGETLTETPWLDTMQTVIDRLSDIHRKHGPES
                     IAGLITARCTNEELYLFQKLMRAGLRTNQLDSSARYGHLNYVHAVRHAVGVGRPLNDW
                     EDLTKAKAILVIGSNITETNPLTAVRIKEAIRVYHSQVIVVDSANTNIAQLASHPLLV
                     KPGSESFLIDGLVKSVIEQDLIDEASTTRHPQAFSALKQALAQVSLEQVSAQTGIAVE
                     QIREIAAIFAEAPRAIALCAEGIVRQPNGYQNVLKLIDLAWVTGKLGQPGCGVTTVTE
                     EINEQGAVDMGVAPEFLPGQARFDDPGARERFEKAWDVTLPASGSGANFLEILARIRN
                     GQIKALYVIGENPLATLPASMDVNGTLEKLQLLIVQDPFLTETGQMAHVVLPAATYAE
                     KDGTFTNLEGKVLRVRQAVDQVGESLPDWHIMTALANGLGYDWTYESPQDVQNEIMKL
                     LPGYYNLGQPRRVSPSADAYLSNGYEAEVASRYHRQTAQSTSSDDTQPFTLLTGQVLY
                     HSGKMSTHASGLINIEPNNGRIRMNPADVERLGLNEQSTIRLTSRQGSVQAGVKADPD
                     IQPGSCFFPEHFNEPAVKDLMAVEVDAVTGVPYFKSTRVKIEKVGA"
     gene            24836..25408
                     /gene="nuoI"
                     /locus_tag="PKK81_01130"
     CDS             24836..25408
                     /gene="nuoI"
                     /locus_tag="PKK81_01130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNV31496.1"
                     /translation="MTKKILQAALFYEIWDAMKVTFKHMFHRPITFQYPREQRTIPDG
                     HRGALGLLRYGDGRDRCVGCDLCEAACPSRCIKVISQEDPERPLQRFASEFYIDITKC
                     VFCGYCVEACPVNALAMTKMYEYSTHDKRTLLFDKKRLYEVGERHLEDAKKYLYAHNQ
                     EKNSDESREYRYYFPQSVLKPTQSQPKHLS"
     gene            25419..25943
                     /locus_tag="PKK81_01135"
     CDS             25419..25943
                     /locus_tag="PKK81_01135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNV31497.1"
                     /translation="MAFLFFAYFAVVSIVAGILTVALKNPVQCGLALLALLLHVSGLF
                     VMLNAEFLWAVQVIVYAGAILVLYLFVLMLLNLKTDDRYFHAKAPYLLAPAAIGLAYL
                     LFLLVRSPFGGSRGDASPAAILLNGDAHAIGIKMFSEYLLQFEIIGVFLTGAIVGAIV
                     LAKTPKSIETRRDA"
     gene            25940..26242
                     /gene="nuoK"
                     /locus_tag="PKK81_01140"
     CDS             25940..26242
                     /gene="nuoK"
                     /locus_tag="PKK81_01140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNV31498.1"
                     /translation="MIPLSAYVAVSAVLFATGLLGVLIRRNFIIVLMSVEIMLNAATI
                     NLVAFSHYLEAMQGQIVALFIIAIAAGEAAIGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            26244..28247
                     /gene="nuoL"
                     /locus_tag="PKK81_01145"
     CDS             26244..28247
                     /gene="nuoL"
                     /locus_tag="PKK81_01145"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNV31499.1"
                     /translation="MSDSIDLIVKLIPLFPLMAVIVNGLFGHRYSHDLAHRFAWGSVL
                     MSFLCVLAVFTETLRTGAAREVVAYKWIFGGDLTINLAYLIDPLTCIMLLVITGVGFL
                     IHVYSVGYMHGESGFTRFFVYMNLFMVSMLLLVMGNNYVVLFIGWEGVGLCSYLLIGY
                     YYDKVSAAKAATKAFVVNRIGDAGFLLAIFLVFVNFRTLDYTQVMQNAAQLSPEMATA
                     IALCLLIGAVGKSAQLPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHAIFDL
                     SPTAMTTVAWIGGLTALFAATIGLVQTDIKRVLAYSTVSQLGYMFLGCGLGAYTAAVF
                     HLMTHAFFKALLFLSAGSVIHALSGEQDIRKMGALKSKIPWTHTLFLIGTIAIAGIPP
                     LAGFWSKDEIMGHAFVHHHYVLYGMAAVGAFLTSFYMFRLTYLTFYGTSRLDHHTAEH
                     VHESPMVMIGPLVVLATLSVIGGFPGVPPENGWFHHFLHSVAGVAGEEHATAPALMLG
                     LMGTATVIALLGWGLAHYFYSGPSSAADALAARMPGVYTTLLNKYFVDELYDRLFVEP
                     TKQIGRLCDWFDRTIIDGLVRSIDRGTDASSAAITWTEKHVVYAGLNIIGYANHRLAR
                     SWRRLQTGMVHQYAAIIVAGLFILVHLILLIWTGSGSNGYLSR"
     gene            28272..29831
                     /locus_tag="PKK81_01150"
     CDS             28272..29831
                     /locus_tag="PKK81_01150"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNV31500.1"
                     /translation="MLEEFSFGFPILSYLIFLPLAGAAVLWLIEDEDLLKTTTLGITL
                     VELALACLVLVRFVPDSAAMQFAEHARWMPALGIGYHLAVDGISVLFVGLTAFLTVLV
                     VIYSWDTVRNQVRLYFMALLALETTTMGIFASIDLILFFVFWELMLIPSYFLIKLWGG
                     GAERHYAALKYVLYTLLGSVFMLVGIALLDINYHQWAVTHHLDHVYSFDLLELLSVPI
                     PLSQQILIFWLMFMGFAFKAPVFPFHTWLPDALVEGPIGMAVMLAGMKLGTYGFLRFT
                     FPLLPDASKSEGVVSIVMVLALAAILYGAVVALVQSDFKRLLAFSSISHLGFVVVGLF
                     ALNFQGLQGSLLTMINLGFSTAGLFFMAGFLSTRQQSSQLSSFGGFAKQVPLLASFLL
                     LIGMASIGLPGTNGFVGEFLILLGAFKAKWWYGAVAVTGVIFGAAYFLWYYERAMLGP
                     LSKNISATIKDLHMREITIALSLSVMILWIGLYPSPFLRMMNGSIQALVDRLDRAKVA
                     SVDTVIHVPAK"
     gene            29836..31491
                     /locus_tag="PKK81_01155"
     CDS             29836..31491
                     /locus_tag="PKK81_01155"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNV31501.1"
                     /translation="MAEYTLLIILFAPFVGALALIFVSNRQLSLVRGIAAGSAFVSLI
                     ASVYLFYAYDPLKGGYQFIQRIEWSRQLGISLHLGVDGIGTPLVLASGILLFAGIFVS
                     WHIKDRVKEFYIWILILAAATIGVFMSLDLFFLYFFYEMSVIPMYLLLGMWGSHTKKY
                     LEMTDAEGLKQRDSVGFIFNFGANSKEYAAMKLVLFLSAFAVAALMGILLIYKFSGLN
                     TFDILVLREQAHFSGPLATLIWLLIFFGFASIAPIWPLHSWSPVGHAAAPAATSMLHA
                     GVLMKLGHFSIIRVAFEILPETTRELMPIAAVLCIFSIIYGGLVAYYAKDTKYVIGYS
                     SSSHMGYVFLGMAALDYISLSGAVIYMFAHAMATGMLFAMAGWVYDQTHTRDIPSLGG
                     LSNRMPFISAAFVIGCMASIGMPGTVNFIAEVMIIVGSWNKYPFQVVVAVLGIVLTMA
                     YLFKMMRGLFYGSMAEKYSHSHDAVTVVDRMPLLIMITVSISFGIFPGHLYSVVRSGV
                     DPLIARITKVVPVAEHPMNLPHATSPTVPTSASREALAKVTPR"
     gene            31488..32984
                     /locus_tag="PKK81_01160"
     CDS             31488..32984
                     /locus_tag="PKK81_01160"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNV31502.1"
                     /translation="MTFSLTLSVSDLLLLLPELFLTAWLCVILIVDFSFKRIVQEQLA
                     YLSILGLVITLACLAWFDVTGITGTLFGKMFVLDRMAIFFKVMILLATILVILLSIDY
                     VHRFAFFRGEYYFLVVMSALGMMFMTSANDLLSLFVTLEFATFGFYVLVSYLRDDMAS
                     NEAGLKFFILGVFAAGLLAYGISLVYGETGKLVFSDMTGAAPTTGLVIGFLLIFAALG
                     FKIGAVPFHSWIPDTYHGAPTPVTAFLSIAPKVAAFAILLRLFLVALATFKPAWALLI
                     VATSILSMTYGNIVAIAQRNVKRLLAYSGIAQVGNVLIGLAAGTKMGTDSILFYLLTY
                     LFANLGAFAVIMAISNAVGSDEIDDYRGLNRRSPFLAFAMLIFLLSLAGVPPLAGFIG
                     KLYIFVAAIKEGLYILITVGLINIVVSMYYYLIVVKKMYINEPLDSSPIKTTGPLRAV
                     IYVGLAGTLVIGIYPQPFLDWAVAATLMFSNLLGPAASIPVPVVPFGG"
     gene            33135..34634
                     /locus_tag="PKK81_01165"
     CDS             33135..34634
                     /locus_tag="PKK81_01165"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNV31503.1"
                     /translation="MSMDEPKPQQAVNTDVESVLPTLPWSARILSRLSILGPLVLAFQ
                     NRSIEQRVLAGFGLVFAGILVISAISYRNMTVLIRNGHHDQRSHEFIQSLAATGEAID
                     DAENGHRRFLVTGDESYLSAYNTLQERAPEYLGYLRELTEPGSIQRARVTQLEQLITQ
                     QLREERAAIDLRKETGFESVRTLALAGVAKTALDSVRRLQAQMEQDETKALAQRVIES
                     TTTTRSSIILLIIGALLLFVLLAAVYYLIRHDITARRRIADELQRRGELLEAANKELE
                     AFSYSVSHDLRAPLRHIDGYASLLAKAASISLDDKAKRYLQTISESATRMGQLIDDLL
                     VFSRMGRQEMLRGTVNLNQLIASVLHDLRHDLQDRTISWTIAQLPEVTGDAAMLRQVF
                     MNLVANAIKFTGTQPQATIEIGSRDNGQQEAVLFVRDNGVGFDMRYANKLFGVFQRLH
                     RADEFEGTGIGLANVRRIIHRHGGKTWAEGALGEGATFYVSLPLARTSA"
     gene            34631..35092
                     /locus_tag="PKK81_01170"
     CDS             34631..35092
                     /locus_tag="PKK81_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31504.1"
                     /translation="MTLTKPILLAEDNPRDAELALAAMEEHHLADKVILCHDGAEVLD
                     YLYCRGHFKARLQGNPAVVLLDLKMPKVDGLEVLRTIKSDAALKPIPVVMLTSSREER
                     DLVESYALGANAYVVKPVEFHQFLKAVKELGVFWGMINEPPPEGASRVTSR"
     gene            35131..>36626
                     /locus_tag="PKK81_01175"
     CDS             35131..>36626
                     /locus_tag="PKK81_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31505.1"
                     /translation="MTQPLRFIHLESNPADAALIATTLRDAGILCQLKQAQTREEFLA
                     ALRQEGFSLILADTAVPGFDGATALSLARTLHPDVPFLFVSDTQGEEFAIDMMQHGAT
                     DYISKQRLGRLVPSIKRTLRELDERLERRRAEDALRVSEKQFRQAQKMEAVGRLAGGL
                     AHDFNNLLTVIMGHSQVLLGELPSGNPIRAKIEEMQKAGERAASLIRQLMAFSRKQPL
                     EPKILPLNSVVGNVESMLRRLIGEDIQLVIRPDPFNGHVKADPGQLEQVLMNLVVNAR
                     DAMPNGGLLAIETSQTELARTPMHHLHPLPLGQYVKLTVTDTGCGMNADVLAHLFEPF
                     FTTKEEHKGTGLGLSTVFGIVTTCGGGIDVWSQVGHGTTFDLYFPRVNPQASTADATA
                     PQARLRQGSETILLVEDDSGVRNLVRHELVKTGYQVIEAKNGVEACLTATQQSYHVDL
                     LLTDVVMPGMNGRELAQHLSVIKPNLRVLFMSGYLDDISVNSGMDPHRT"
     assembly_gap    36627..36726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <36727..37317
                     /locus_tag="PKK81_01180"
     CDS             <36727..37317
                     /locus_tag="PKK81_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31506.1"
                     /translation="FREDYSYLGVLLKEGRSLGTKKHHSWQGLFAAEPTDGRLRKARV
                     LDLAFDGFTEEAPPYREAAQSLMLFDRTGKLRVRDSGKRAFQTVVAEEGEGAILVIKT
                     VDIVSLHHLADCLHRQIPSIQQAMAMDGGASSDVIASPDLLHAAQETTSQATWRSLLA
                     GNIGVHIPLPTVIGISPRTHPRTMPAPDASTSSHSR"
     gene            complement(37329..37754)
                     /locus_tag="PKK81_01185"
     CDS             complement(37329..37754)
                     /locus_tag="PKK81_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV31507.1"
                     /translation="MRYLSGLLCVGVLCGLLLEWPQPTFAYHSYTLTVQQLRAGLLKA
                     PSTGAKGFLLVDVRSPEEHASGMIPGTDRNIDFREMKARHREIGAALNDHIVVYCQSG
                     HRSNIAAETLADLGYTHVYNVVGSMNAWTEAGFPVSPGR"
     assembly_gap    37845..37944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<37945..38345)
                     /locus_tag="PKK81_01190"
     CDS             complement(<37945..38345)
                     /locus_tag="PKK81_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31508.1"
                     /translation="MGKTVMLQDDLIAAFHKTQSFKWDPKGGFKLASGLTSPFYVDCR
                     TLMAFPHARHLMAQRAWEVTKGLDIDCLGGLEIGAISIATTISDFAYLATPRREWRTF
                     FVRKQPKDHGLGRLVEGVVQAGDRALIVDDV"
     gene            38768..40351
                     /locus_tag="PKK81_01195"
     CDS             38768..40351
                     /locus_tag="PKK81_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNV31509.1"
                     /translation="MCRLLSRLGRDFLIVTCLWTISATHAAEQPAAPAAVLVKGFSFS
                     GNTVISQAELEALTQPYVEQALTLPDLERIAAAVADLYKQRGYTLASAYIPQQRIRFG
                     MVEISVLEGRLGDISVSGNRFYSTEFIRSGFAGVMEARVIRNTDLERALLLLNDYPDL
                     KVSAALEPGEFTGSTNVQASVEDKRPIHATLDYNNYGFNNISRNRFGAGIEVGNALRE
                     GAILTFNGIVGDHPERLQFITGGYALPVGVHGTKVVLSGSTGRFDVGAELAALQIRGK
                     IHTYDLSITHPVVKTRWQSLVLDTGFAAKDNRLFINGALTGHDTVRMAKLGMNYDRVD
                     LYGRTYLSAYGFHGLGEILGGMDDHAAISTRPGADNRFLKGTLAVGRIQSLGHDALLI
                     LKGTGQITSGPMVVIEQMLLGGPDSVRGYQLGERFVDEGYAVTAETRIPLLPSMLPST
                     QAALFFDHGAGHLRNPQPGERRYSSLSGAGVGLQTELPYYSIHLRADLGFPVGPKPSG
                     GTIAGDRSPTMYLQAATRF"
     gene            40351..>42170
                     /locus_tag="PKK81_01200"
     CDS             40351..>42170
                     /locus_tag="PKK81_01200"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNV31510.1"
                     /translation="MYDSPIHKTARAPKTGPASFAFSEPLLRRHRILCGLLAAVQLLI
                     PSLTLALPTDGRVAGGGATLQSTSKSITIQQTTDKAILNWKSFSIAADEAVHFRQPSA
                     DSIALNRVIGGDPSIILGRLQANGRIFLLNPNGILFGAGAQINVGGLLASTLHTRDEE
                     FMAGRYLFAQDPLKGLKTVVNQGTLQVSEHGFVILAAPAVSNEGIIVANLGTTVLGSG
                     QQLTVDLMGDGLINYALTGKVLEQVTGIDGTASGSAVKNSGTIHADGGQVILQASAAG
                     DIFSSVVNQSGVIRARSLTHEDGIVRLDGGDSGQVTLAGTIDASGPASGQTGGRVSLL
                     GESVTLTPTARIDVSGDGGGGTALIGGQFQGRGTEPTATTTLVADRASISADALTQGT
                     GGTVIVWADGHTQYAGTISAQGGASGGHGGFVEVSGKQALDFTGRVDTTAPAGHTGTL
                     LLDPSNITISSAANANISAAPTFTGTTATSTLNVTTLQTALATNNVIVDTTSPFASAG
                     NITVSNAVTWSSANSLDLRAHNTITVNAGLSSTGLGILRLIGDQDGIGGGNVALNAAI
                     SARLGGIEISGDGITSGTAGTITTTGLASGDAGHITINATG"
BASE COUNT         8731 a        13117 c        11586 g         8536 t
ORIGIN      
        1 ggcggatact gcgcgcgcat cactggggca ggagccgcca tacgctcttc catcgtcgga
       61 ggctggtgac tttgtgcaac cgccgacccc gctgccgctt gccccggcgt gaccgtcgcc
      121 acacgcggtt tcgttggttg agtcgcttgc ggaccttgtg gcccagccgc agccatcaga
      181 gccgctgtcg tacctggagt cagctcaggt cccggcgtgt atggagaggt ggctttagga
      241 gccgccgccg gcgctacggt ggcgggctgc gcaacctgtg gaatatctgc cgccggctga
      301 gcctggatct gagcaggcat ggcagcggtt tcgttttggt cgggagcgtt ctcgggaggt
      361 ggaatatcat tcacctcctt cttaaaccct ttgagcgcct tgccgacccc ctcgccgatt
      421 tgcggaagtt tcccggcccc aaaaatgatc aacacaatga ccaggatgat aatcaactcg
      481 gaaaatccca tagtgccaaa catgttcgat atccttcctc ggcagatagg agtcaatcca
      541 gcgtcagagg gcctcttcgg ccctctgaac tcccacgata gctgcccgat gcaaagggtg
      601 tcaagaaagg tgggcggcca cagccggccc aggaatagca ccgaagaacc tagctcgcag
      661 ggatgggaat cgggagaaga tcggcctgaa ccggctggcc gtacaccacc gcgtcgcggg
      721 cggagagata cacatccagt tcactccgaa taccaaactc atccggcagg tagatgccag
      781 gctcaatcga aaagcaggtg cccggcaaca accggcgcgc atcctgcgtc tccagattgt
      841 cgatattcgc gccgttgcca tgcacttcct caccgatgga atggccggtt cggtgcacga
      901 aatattgccc gtaccctgcg tcctgaatca cccggcggca gacatcatcc acttcccacc
      961 cataggggca ttcaccggct cgtacacgac cttgcacaaa ggtgacggcc gcatcccgag
     1021 cccgtcgaac aatctgaaaa atgtcctggt gccgggcagg cacagtcgcc ccgacgaacc
     1081 ccgtccaggt gatatccgca tagaccgccc ctggtccagg ctgtttagcc cacaggtcga
     1141 tcaatacaag gtcaccctgc cgaatcgggg ctgatccttc ggaagccggc gcgtaatgag
     1201 gatcggcgct atgcgcattc accgcagcaa tcggcggact cgaggttacc aagccacggg
     1261 cctgcatgcg ggaaagaatg tattgctgta atccatattc agtcaggctg gaccgagcag
     1321 ccagcgaagt tccgacaaat ccaaacgcct cgtcgacaat tgcgcgtaga ccttcggctg
     1381 ccacctgatg cgacgccaat tgtgcatcat cccacaccgc ctcaaattgc tgaaccagat
     1441 cggcagaggt caccacctcc gcgccgagac tgcgcaccag atcaatcgtg ccggcatcca
     1501 cgcgtgacag atagggaatg gcattcatcg gcgaatattg catcgcgatc cgcttggcag
     1561 ggtgcagcag ggaaccgaga gccgcctgct gatcgcgcca agagacatac gcatgcgcgt
     1621 ctccgggcaa cccatccagg acatgaggct caatgcgatg ctggagcttc accggcgtac
     1681 cctgcgcggg aacccaatag taccaacgcc gcgtgacatg gagcgagggg tctaacaaca
     1741 agacgcgata cgcgatgggg tcgaggtggc gaaagtcgta aaagagccac ccatcaagtc
     1801 ccggctgctc ccggatggcc tgctgaatac gaatcacccg tgcgtgagaa cgatcggcca
     1861 cgttcatgct tgccatcata gcgtcattcc gccagcgata ccactgggca atcacgctgt
     1921 tctctgccct ccacgcaatg ttagaatgaa ccgaacccgc tatggctatc gttccctcat
     1981 ccgatcccgc gcagtaccgc gtcaccttct tcttcgggcc ggaagccgtc gaagagcgac
     2041 cggatcatct gcgatgcgtc ttcaacgtga agaagcggag ctggaaaggc ggcgtgcaag
     2101 tggctgtgga tgtggcccgg cgtcacctcg accgggcccg cgagcgaatt gggtatgcct
     2161 cctggctggc tgaactcata cacagcggcc accaagagga gcgggaggag ctgaccagcc
     2221 gcgccgatga tctcttcgtc cagtcgctct gtaccagtgc gctcaacttg gccctccagg
     2281 cgggaattga acaggtgaat caacagatcg acgccgccac gttcgccgac gaactgcatc
     2341 aactcctcga cgaacaaccg gaacaggtga ccgatcgaat tcgcctcgaa ctggacctgg
     2401 gtgagacttc cgaaatctaa tgagcaacgg tgtggcagca aagccggaag gggtttgaaa
     2461 acggggggga atttgttata acgacaacgg actcatcggc attcattttt ctttccaccg
     2521 catcagaact tcagcacgaa ggaggcaccc aacgtgaacg tctctgtcta ttcgcccctt
     2581 aaaagacgac gcagcctctg ggctcaagtc ctcgtcttca gtctggtcac agtctgttct
     2641 ctggctttac tcatcgcccc cgtctccgcg caggagtcgg gaggcgcgtc taatgtgccg
     2701 gtctcgtcga acgatgccag tccgtcgggt gtcggcattc aagcgggctc cgcactggcc
     2761 accatcgtct atttcccgct caaactggcc ttcgcgatcg gcggcggggt cgtcggcgga
     2821 ttggcctatg gcttctccgg aggcagtgaa cagacggcca agaatatctg gatccccagc
     2881 atgtacggca cctacatcat cacgccggat catctcaagg gcgataaacc catccgcttc
     2941 ctcggcgtct caggggagcc tgcgcgagag gccggcgatt acgccccgca agaaccagcg
     3001 ccgctgcctt tggaaccgat ccgctagcat gaaacccgtc atcggagtca cccccgactt
     3061 caatgccggt gatcgtcaag aatggggcgg caaggagccc acatattttt tgcgggcccg
     3121 gtatgtccgc gccattgagg aactcggcgg cgtgccggta attctgccac ttgtggcgaa
     3181 ccgatccgca cgccggcgcc tgctggaagg ggtcgacgga ctcctcctga caggaagcgg
     3241 gccggacttg gatccaaccc tgtacggaga aaaaaagcga tatacctttc cggtcgtcgc
     3301 taaacgacgc tccagctttg aattggactt ggtgcggtta gccatccgga accacatccc
     3361 cacgctggct atttgcggag gcatgcaaag catgaatgtc gcctgcggag ggagcctgta
     3421 tcaggatatt ccggcccagg tcgaccatgt gttacagcac cgacagacga caccggcggt
     3481 tcgactctct cacagcatct ccatcacacc cggcagcctg ctcaatcgca tcgtcaagcg
     3541 cgcccgcatg caggtcaaca gctcgcatca ccaatcggtg aaagcagtcg ggcacgccct
     3601 tattgccagc gcaaccgcgc cggatggcat cgttgaagcg atcgaacttc cgacgcaccc
     3661 cttctttctc ggcctgcaat ggcaccccga atttttgttc gaacgccacc tcttgcaccg
     3721 acgactcttt cagacattct tgcgggccgc ctcgcgccgc ccgtcactcc aaccttcgcc
     3781 tgcgagacgc aggaacaact catgacacgg ggttcatttc gccataacag cggacatgga
     3841 cgtggcgccg ccggagagag gacactgcgt gggcaatccg ctgtttcgca ccaaatccat
     3901 cgaacgaatc ctcgctgatt ccgacgcccc tgaacatcgc ctgaaacgga ccctgacggc
     3961 ctgggacctg accggactcg gcatcggcgc gattatcggc accggcatct tcgtgctgat
     4021 cggcaccgct atcgtcggtg atgcgcaccg gccaggcgcg gggcccggca tcatgctgtc
     4081 gttcattctt tcaggcatca cctgcgcgct cgccgccctc tgctatgccg aatttgccgc
     4141 catgatcccg gtcgccggca gtgcctatac ctattcttat gccaccttgg gagaatttct
     4201 ggcctggctg actggctgga atctgatcct ggaatatggc gtggcctgcg tggccgtcgc
     4261 gatcgggtgg tccggatact ttaacaacat cctcaaactc tgcggccttg aactcccata
     4321 ctgggccact catcctcccg gcgcggacgg cggcattgcg aacattccgg ccgcgatcat
     4381 cgtgttgctc gtcacctgca tcttgattgt cggggtgaaa gagagcgcgc gcgccacctg
     4441 cggcattgtg ctcatcaagc tggccgtgat cgccttcttc attgcggtcg gcacatcctc
     4501 cgtcgacacg gccaattggt cccccttcat gccgttcgga tttgccggcg tcggcgcggc
     4561 cgcagccatc gtgttcttcg cctatatcgg attcgacgct gtgtccacca ccgctgaaga
     4621 ggccaagaac ccccaacggg atttacccat cgggatcttc gcctcgctgg ccatctgcac
     4681 cgtgctctat atttctgtgg ccgcgatcct gaccggatta gtgccttatt cgcagatcga
     4741 cgttcatgcc ccggtagcgg aagggctccg catggtcgga ttcaagtggg gagccgccat
     4801 cgtggcaacc ggtgcggtag cgggcattac cagtgtcctg gtggtgatga tgctgggcca
     4861 gattcgggtc tttttcgcca tgtcgcggga cggtctcctg ggtccctggc tttcaggcgt
     4921 ccatccgaag tttcgaacgc cccaccacgc cacctatttg accggcgtgg ccgtcgccat
     4981 catggccgca ctcattccga tcggtgaggc agcggatatg acgaacatcg ggaccctctt
     5041 tgcctttgtc ctcgtctgta tcggcatcat ggtcttgcgc tacacgaagc cgaaccatcc
     5101 acgccccttc cgtatgccat tcatgccggt cgtccccatt ttgggagtcc tggcctgcct
     5161 gggcctaatg tatttcctgc cctggatgac ctggatccgg tttttcgtgt ggaccatcat
     5221 cggcgtccta gtctacgcca cgtacgggat gcgccatagc aaactcgcca caaggccctc
     5281 gacagaacag tccgagacac ctcgaccggc atagtcgaat tacgagccaa aaaactggtg
     5341 acgggaaaaa ctaggacgat gcttcggttg gagcaaccgg ggaggttgtc ggaggtgctg
     5401 ctgcagccgg ggccgcaggc ttttgggcaa ctgccgcagg tggaggagat gctggcgccg
     5461 gagtcgaggt acctgctgcc gcaggagcag gctgagcagc cgcaggcttg ggaggagcag
     5521 ggggcttcgg cggagcaggc cgctccggtt taatcccgcc ttcccaggaa ggacctgaat
     5581 acatatcggc ggccagccct ttgcctttcc acttttcttc gaaatctgca gccgctttgt
     5641 tcggggtttc tcccgcaccc accacgtcat tccagggata ggcccgggga ccaatctggc
     5701 acccggtctc cgtacgcctc aaatagaggg caatcgggtt cccccggtac ggcccgagat
     5761 agatatgcac tgaccccaca tattcgagca atgtggccac cgcgcctcca agaagaggtg
     5821 catgatgcca taagaggcgg gaagagggca agatggacct atcacccccc tgctatttgg
     5881 tccagaagcg attaggtcgt aacggggcgc tctctcgtag ggcgcatgcg cagcaggtac
     5941 acccccaaga tgaggacgat gataacggtc acgttgaggc cgacatccat cacatactgc
     6001 tggaagagtt cctgccgcgc ctctcgcgtc accacctcaa gctgatggga taattccgcg
     6061 ctggccgtca acacccgtcg cgtgcaggcg atgatgccga ccgccagata gggctccaat
     6121 tccaacacct cggtctgaag aaaccgcacc accgtacgga acaattccag caggatgatg
     6181 acgagcagca gatcgttcaa caacttgagt ccggcaggca gcagcgggac atcgttggag
     6241 cgcaagaaaa aggcgtacca ggcatgaaca aaaatcagca tgcctaaggc cagcaagctg
     6301 aatcccgccg cgacatagcc caaccggtct aagtgatcca tgaatcccag caaccactgc
     6361 atggttgccc ggcgagactt ctccaaaggc ccgggagggg tcaccggcgg cgtcatgctg
     6421 ttcctgcctg accggtaggc atgggggaat cctccagtgg ttgaccgaat ttcaaaccat
     6481 tgaccgcctt ggctgtgatg gtgtggccac aacaatcaat cccctgaaac ccggcaccgc
     6541 cgtcgatcac catcaggcgc aggccgcagg agtcggggaa ctgccgcttt tcctccagca
     6601 tagccccggg gagcaatgcg ccttttttcc ggcctctcga cgagatgata tcggagacca
     6661 catcttcttc agtcagctca tgcccgcaac agaggatctt ctggaaaccc tcgccgcccc
     6721 cagccacctt gaccaccaac ccgcacgaat ccggaaagcg ctttcgctcg tcgagaatgt
     6781 cacctttttt gattgccatg ccgcctcggt atgaaaggaa agacgtgaaa cgtcacatga
     6841 ttcgatagtc aatcagatcg ccgggggaaa agccagcgca gactctatcg gccgagccca
     6901 ggatatggag agcctggatc gcgctgtaga aatcaggccg gcgccatgaa ttgagcagaa
     6961 aaagaacacc cactcccccc gtgcctggag tgagaggcga acggaacagg gcgcacgcag
     7021 atcagacgtg cgtcactgga gaatcatggg ctggtgccgc ctgacccctc tcaactaacg
     7081 tcggtttggg gatgcgcgca taggccttgt gccatgagtc gagcagcacc ttgtgcgacc
     7141 ggcacccaac caccaaccgc gcatcggaaa tcgtgaccgg cttgtaggga tggaggtacc
     7201 gtgccttgag cttcgccaca cgggccagcg actggtccag cctctcctga gtgatcctgc
     7261 catctttcac cgcacgctcc atcgcttgca tggcggtcat gacacggtcc tggtccttgc
     7321 agatcaacag gacatcgcat ccggccacaa acgaccggac tgccgcttcg ccgatcccgt
     7381 cgtgatcgat aatggcatgc atctccagat cgtcggtaaa aaccacgccc tcataccgaa
     7441 actcttcgcg caacaagcgc tggatcaccg ccgaggacag ggttgccggt gcctccggat
     7501 ccaaggcccg atacagcaca tgggcggtca tcaaactggc tacgccataa cgaatggcct
     7561 gctgaaaggg aggaaactcc gtatcgcgca accgctggag ccccgcatcc acgacgggaa
     7621 gctctttgtg cgaatccgtc gccgtgtctc catggccggg gaagtgcttc ccgcatgcga
     7681 ccaccatatt gtcctgcagc cctccgatgg tcgccgaccc catctcgccg acaagctcgg
     7741 gagcagcgcc gaacgcccga tcgccgatga cgggattgtc cggattgctg ttcacatcta
     7801 ggaccggagc catgttcata ttgatcccca ccgcccgcaa ctctttggca atggtcgcgg
     7861 cagcggagta ggccagttca ctggagttgc actgtcccag ctgtccgcag ggagggaaaa
     7921 tcgtaaattc ggcgggcagc cgggataccc ggccgccctc ctggtcaatc gcgatcagca
     7981 gcggctgagc gggcgacagt ttctgcagcc cgttggtgag atcgacgatc tgctgaaccg
     8041 actcgagatt gcggcggaaa aagatgacgc cgcccggctt ggaggctgtc agaaacgagg
     8101 ccaggtcttt ggtgaccgtg gtcccggtga aacctatcat aaaaagctga ccgatctgct
     8161 cgcgtaatgt catcactcgc tactccgacg atctggtgag aagcaggcag atcccccgtt
     8221 gcgttcacgt accgaaggca ggtcctacgg caacgtgcgg tccaggagga actgaatgag
     8281 gaggcgtgtg ccgatcccgg tcggaccctt cggcagatac gctcgctcac gttcgctcca
     8341 gtcggtgctc gcaatgtcca aatggatcca tgggcaatcg ccgacaaact tgctcaggaa
     8401 caaggcggcg gtgatcatcc ctccgccccg gccgccgata ttccgcatat ccgccacatc
     8461 gctgcgcagt tgttcgaaat actcctccca cagcggcatc tcccacacgc gctcgccggc
     8521 gcgctggccg gcgttccgga catgttcttt caactgatcg ttgttgccga acatcccgat
     8581 ggcaaattgg ccgagcgcca ccacgcaggc gccggtcagg gtcgcaatat cgatcaacac
     8641 cgccggtttg taccgggtgg cataggccaa cgcatcggag aggatcagtc ggccttcggc
     8701 atcggtgttc tgaacctcaa ccgtttttcc ggacagcgtc gtcacgatat cgcccggccg
     8761 catcgcgcgc ccgcccggca tattttcggc tacgggcaga atgctgacga ggtggagcgg
     8821 aagcttgagc cgcgcggcag cccgcatcgt cgccaggact tcggcgccgc ccgtcatatc
     8881 cgccttcata tgttccatgt tctcggccgg cttcagcgaa atgccgccgg tgtcaaaggt
     8941 gatggtcttt cccaccagca ccaccggagg atcgcccttc ttcgctttgg cgcccttgta
     9001 ttccaggatg atgaacttcg gcggttcgtg gctgccacgc gccacaccca gcagggcacc
     9061 catgccaagc tgctccatgt ccttctgttc gagcaccttc agacgcacac cggattcttt
     9121 ggccaccgcc ttggcctcac gtacgatgcg cgtcggcgtc atcacgttgg ccggatggtt
     9181 gcacaggtcg cgaaccagca ccgtcgcttc agcagtcgcc acaccgcgcc ggatgccctc
     9241 ggtcatttgg gggagcagcg aggcttgcga cgtgtagatc gtcaaccgtt cgacgtcgat
     9301 cggcttgctt ccattggggc tacgataggc ggtgaattga tagttaccga gaatggcgcc
     9361 ctccgccatg gcctgcgcca catcctggac cggaatctcc tccagaatgg cggccggcaa
     9421 cacgacggcg aaggaggtca ctttcgcctg acggacacgt ttgatcgcaa taccaagcgc
     9481 ctggcggaac gcatccacac ggagatcttt ttccttaccc aatccagcca acaacagccg
     9541 ttttgctctg gccttgccct gggtatggac cagcaaccct tcgccgagct tcccttcgaa
     9601 ttctccgcgc tgaatcaaag cggcaagctg tcccccgagt tgagcatcga tggcggcagc
     9661 ctcctgcgtg agatcagagc caccctcgca atggagcaac accagaacct cggccgcctc
     9721 gttttctgcc cgtccaacct gtgcgtgaac ttggatcttc ttcatcaatc ctcccctggt
     9781 tcttgccgtt cagcgttcat catcagccgc tcacagactg ccccgtcctt aagaatcaca
     9841 cccctctcaa acgccgcgca tatccggcgc ccaaatgtat ttgtgcattt ggagctggaa
     9901 ccgcaccggc aacttatcgg ccagtatcca ctccgccaga tgccgcacct ccacttcgcc
     9961 gaacaccgga ctcatgagca ccgtgcaccg cgccggaaga tcgtaacgat ggagcacgtc
    10021 ccgcgcccat tcatagtcag atcgatcctt gatcacaaat ttcacttcat cgtgaggtgc
    10081 gagccgatcg aggttcggcc aatacattcg atccgtcatc ccactcccgg gacatttcac
    10141 atcaagaatc acatggacgc gccgatcgac accggccgta tcgatggccc cgctggtttc
    10201 catcagcacc tcgaacccct cgtcacacag gcgactcagc aagggcagcg actccgtctg
    10261 gctcaacggt tcccccccgg tcacttccac aagatcacaa tcgaaggcgc ggacctgatt
    10321 gacgatgtcg tcgatcgtca tatcccgacc gccgtagaag gcataggccg tgtcgcacca
    10381 agtgcaccgg agcggacacc cggtcaaccg gacgaatacg cagggacggc cggcataggt
    10441 agactcgccc tggatgctgt ggaaaatttc ggtaatcttg accatcgccg attgactcag
    10501 cggctcagca tcgcactgat ggatgaatcg ggggacttat ttccatctcg tcacaggcca
    10561 tccattgcgt cgcgcaatgc gatccattcc gcgaatcgga ttcacaacca atggatgccc
    10621 caccatttcc agcaactcaa catcgccggg actatcgcca taggcaaacg actgcgtcag
    10681 atcaatgcgt gattcctgtg tcaaccgggc gatcaaggcg cgcttgccgg ggccataagg
    10741 aagggggaca cagacctggc ctgtaaaacg acgctgttgc cgttccagtt tcgcggccag
    10801 cagggtgggc acctccagca aggtggccaa cggagcaatg aggaagtcga gggatccggt
    10861 caccagcacg atatggtgcc ccgcccggcg atgttcgtcc atgcgagcga gcccctgcgc
    10921 cgaaagacgc ggaaacagct cggcacgaca gaactcctca gctagcgact caacctctgc
    10981 cgcagccttc ccggccagat aaagcttccg ttcccgcagg gggtgcaatg acaccggagg
    11041 cgcattgcga agcacccagc caacactatc ccgcagctcg cgccacccga caagaccccg
    11101 cttccagaga aagcgaaaaa aacggacttc gctcgcctgc cccgggagca aggtattgtc
    11161 gacatcaaac aacgcgacaa tgtcagtctg ccggctggtg cccgaaatat gtgagagtga
    11221 gagaggtgtc atctcgattg ttggcctgct gatcgctgtg ataaggaaga atctgcgaca
    11281 ggtccgcaca gcacgcgtga attctactgg acaactagtt gattgacaag cattttggac
    11341 cacttctata atgcgcttcc tctgaggcct ggcaccggcg tagcaagaat gcgcgtttca
    11401 ccgccgtcta gcttgtgtgt acctcgcttc atgtttcgac ggagagactc tcggttgccg
    11461 aagtggtgga atggcagaca cgcacgtttg aggggcgtgt ggcgcaagcc gtgcgggttc
    11521 aagtcccgcc ttcggcacca aactcaacag tcgttcgacg aactcaccgc ccatcagcga
    11581 ctcgccgcaa atacggcacg ttcgtttcag caccatcttt cttcaggcgc ttcttctctc
    11641 cggcagggtg cacccgacac gagcacctgc accatccggc tcattgtgca gccaccagcc
    11701 aggatcgacg gctctgcaat gactcctctg ctacagataa acggccctat tccactaggg
    11761 cctaattaca ttcccaattg tcaatcatct ctaaccgcct actccggctt ggtgctatga
    11821 tgtgcggcgt gcaagcctat tcactggatg gtgagtgcga cacttcatac caaatgaacg
    11881 ttccgccgaa gcagtcccgc cacccctctc gcatccacgc atggtgaggg aacgtccact
    11941 aaggatgcag cccgctcata gcagacgaag gaggcacctg tgaccgcgca agacgtgaca
    12001 acggccctcc cgccgccggg agatttcagc accgttgagg cccttgtccg aaccctgtcc
    12061 ggtcaagcat ccatcggcat ctttctgtcg gaccccgaag gccgcacgct gtacctgaat
    12121 gagcggctgc gccgcatcgc cggacttccc gtcgtaccga cgccagggga atgctggcgc
    12181 aatgcgctgg cacccgaaga ccatgaccca atctgcagcg aatggtctgg agccaccaac
    12241 gccgagcgca gcttttcacg tgagtttcgc ttccggcggc cggacggctc gctgcgctgg
    12301 gtcatggcgg aagccttccc attgcgcagc ggcggaggac cctccggcgg ttacgtcggc
    12361 atcgtgcgcg acattacccc cagacaactg gcactggacg ccctgcacgc aagcgaggag
    12421 cgctatcgga cgctggcgca acagtcgccc catgcaatcc tcgtccatgc ggactctgcc
    12481 atcctgttca tcaatgaagc cggcgcccgg cttttcggac tcgcgaccgc acaccccatc
    12541 gagggccgcc cgctgtggga tttcttcccg aaagagtttc tccacgacct ccccttggca
    12601 gaagccgcgc ccagcccctc agtcgagcga caattggtac gacccgatgg aacggtcatt
    12661 gatgtcgaat tgacagcagc cccggtcatg ttcgatggac atccagccgt ccaaattctg
    12721 ggcaccgata tcaccgaacg gaaggtcatc gccgggcaac tgcatcgggc aaaaaaaatg
    12781 gcgaccgtgg cgacgctggc cggcggcatc gcccatgaat ttaacaattg cctgaccgcc
    12841 atcatggggt tctcggactt ggcgctgccg tcactcgtgc cggacagtcg cgtccatggc
    12901 cacatccagc aggtagtcct cgcgtcgaaa cgggctcgtg atctcgtcac acaaatgctc
    12961 atggtcggtc gacaggcgga cggcgcgaaa cagccgctct ccctggatat cctcctcaaa
    13021 gaaacactgc gagtcctgaa gatgaaactg ccggagtcca tcagcctccg cgaatggatt
    13081 cccggcgcaa ccaaccccgt gctggccgac ccaacccaga tccatgaact ttgcctgaac
    13141 ctgctggcac ggtctcaaca ggccatgacg atcagcggcg gggtcttgga ggtccgcctc
    13201 gacaatatgg gtcttgacgc ttcgatgacc ggtcacgacc ttccgctccc accaggtcag
    13261 tatgtgcgcc tcatgatctc ggataccggt gacggcattc ccgctgacat ccggacgccc
    13321 aacatgtatc cacttttcgc acccccctcg acgggaagca aagcgggagc ggggctcgcg
    13381 gagttacagc gaatcgtgag cgagcatggc ggaacactcc gcgccaccag cacagccggc
    13441 cagggcacca ccatcgaggt ctatctgccc gcaatgcttc agccccctca gacagtggct
    13501 caggaaccga gccgcgagcg caccgcaccc cccacggaag cgaaggaatt tcttgccgag
    13561 catgacaaag agcggtaaat tcactgtagt aattaaacgg tgtcgaaaag ccgccaccgg
    13621 ccgtctcctg agagccgcaa cacaataccc aaacactcga acacgaatac tcctgtgagg
    13681 aatgttatgc cctcggtcct gatcgtggat gatgaagaag cgattcgccg actgattcgc
    13741 agcacgctgg aacaggcagg ctatcatgtc cgcgaagcgg ccgacggcaa agcagggctg
    13801 tcccactatc gccaatcccc ggctgatctc gtgatcatgg atatcctgat gccggaccag
    13861 gacggccttg agagtatttt gacgctccgt cgtgaatttc ccgaggccag gattatggcg
    13921 atcaccggcg gaagcgacat gatcggcatc ttaaacttcc tcgatgtggc ccgtatgctg
    13981 ggcgcgcgcc gcacgctcca gaaacccttc gagatgcagc agctcctgga tgcggttcaa
    14041 gccgagatcg ccggctgacc gacgcctgcc tcatctagtc cgttcgtcat accgccccac
    14101 tgtcacattg acttccggca catcgatccg cacactagcg cccgctttcc gatccccttc
    14161 atacatacaa gaaagatgtg atcatgaaga cgtggtggac tccgcagcta cacgcggtat
    14221 tcggcggcat cgtcgtcgca atcggggttt tcgtcatgtg ggagcaggcg gcgaccacct
    14281 gggccctgct cgtcgccact gcatcgactg cctttctctg gtggcaggga cgcagcatcg
    14341 cggccgtctg ggcctggacc accctggggc tcgggatcga aagcatgacc tggccgattg
    14401 tgaccatgat tcgaatgagg atggggggaa gtcagccgac cgaggagcag atggaaacga
    14461 ttctgaacgc cgtgctgttc gggctgttca catcggtatt ctggatcacc ttcgcgtttg
    14521 gactattcaa gcgtatacgc gaacagaacg ccccgccggc accaccgccc ggaaacgggc
    14581 cggtcccggc acggagcaag aagaaacggg ccaggcgctg actctcgtca cgccatggca
    14641 accggatcga catcgatatc gaaccggagt ccacctcgtc gtgattcccg atcgagcact
    14701 tcacgggtcc gcacgactat ttctttgcct acctcaagag acagagactt gaccagaatc
    14761 tgccaacaat agcgacctcg cgccatggca tgtggtgccg gagacggtcc caggataacg
    14821 atgctgctcg tctctccacc gaaaccagtg gaacgctgcg gcagaggcga accgtcgccg
    14881 gcattggtcc cccgatttcc ggcgctcacc acctccgccc gtagcaacgc cgcccagcga
    14941 ccggcggctt gggcgaccac cggttccagc gtcccggaca catccaacct cacaagcgac
    15001 gtcaggggcg ggtactgaag catctgccga aaagcctgct cctgttgaag aaacacggaa
    15061 tcgtcaccag cggccaccgc cataatcgca tgatgatccg ttaaccgggt ctggatcatc
    15121 accgcgcctc cggcatgagc gggcagagct aactccacgg catccatcag gccatggtac
    15181 atccgttccg ccgagcgaaa gtccggcacg tgaagaccgg catccgcatc cggaaccccg
    15241 acaaacgccg cgcgtgcctg aaggccaaac cggaacaaca tctgcgtgcc gatcacgatg
    15301 tccaattcac cggcggcaag cagccgactg aacgcccgtg catcagcagg tcggcgaatc
    15361 gtctcaccat ccacacgacc cacccgcgcc agagggaact tccgccgtac ggcctcttcg
    15421 atacgctccg tacctgagcc aaccggctcc aacttgagcg attggcaccg agtgcaatga
    15481 tccggcacgg gtttcgttcg cccgcagtaa tggcaccgca catgattgct gcgccggaaa
    15541 aacgtcaatg caacgctaca cgcatcgcac tgcggcatcg cgccacagtc cccgcaatgc
    15601 aagacactcg caaatccttt gcggttcaga tagagaatcg ccagcgcgtt ctgccgcagg
    15661 gcttcctgaa tgccctcata gagcggcggc gataacaagg ttcccgccgg ggtctccctg
    15721 gagtattccc tcaaatcgat gacctgcacc ctcggaaaac ctgacggatc tcggtagaca
    15781 caggctgtca tctccccttg ctgaaccgcc gaccaactct caagcgacgg atggctcgag
    15841 gccagcacca acagggctcg atcgcagaag gcccgccgcc gtgccacctc ccgcgcatgg
    15901 taccggggcg attgctcatc cttaagggac gcatcgtcct caccttccac ccacaccaat
    15961 cccaatcgat cgatgggcgc gaacaccgcc attcgggtgc cgaccaggat cgtggccgac
    16021 gaatcctgtg ccgattgcca cactgctgcg cgttcccttg ccgaaagacc actgtgtagg
    16081 agcaacggcc gttctcccgc tgccgccagg gcttcggcca atcgaccggc attctcgacg
    16141 tcacccgtga cgaccaacac acgccgccca cgacggatag cggcccgagc tgcctgcaca
    16201 agacaccaga gtctggtggc ccgagcccct tgaaccagga acgagccgta tacgtcctgc
    16261 gaaagcgccc ggccaacggt atcaggccac gctgccgcct ccagtggcgg cggcaagtca
    16321 tcaactgtca atctcgcgaa aaccgcctgc tccgcccccg gagaaggggg tttttttgct
    16381 cgagccgccc tcgggacaac cgcgtcatcg cagcgcacca cgagccctct tcgaatcagg
    16441 atctgcaccg cgcgcatgac acgggacttg tcaccctgtg ccagggtacg ctctgtaatg
    16501 cccttgggac gacgacgaag gcgcgagagc acctgagttt ccacttctcc caagccctca
    16561 ggtaacgacg aattgtccgt gccctgcgcg gtcggccgat accgtgatgg ccgccgatcg
    16621 acctgatcga caaaaggcgc cacaagcttg atgcattgcc cccacggtgc ggcataccgg
    16681 tcggccaccc accggctgag atcgatttga ctggaggtga gcaggtggtc tggagacgct
    16741 ggggcaaggc agcggacggc tttcagcccc tgctcgggag cgccgagcgg aaggcggtca
    16801 tacaccgccg tgacgagacc gtgaaggtcc tgggacccaa acggcacgat cactgattgc
    16861 ccgattgcga cctgcccctt gagtgaagac gggatcacat aggtaaaggg ccggtgaagg
    16921 cggcgcggca gcaccacatc ggcataccta gggggaagcg caggagccac cggtggagtt
    16981 gcaaacgaac ggtgatcgaa catgcggcat tctaacaaca ccggatgaga gcaacaatca
    17041 cggtccttgc gagaaggggg ctgacgggac gccgtagtgg aagcgggggg gggctatctg
    17101 ccaggagcga ggatcaacac acctgtggcg acgggcctcc tcggaagaga cccgtcgccc
    17161 gtcactcatt acgaaccggg cgggggcgag gccgtctcac cgccgacagg cgaaaccggc
    17221 actgcgacat ccgagggagc gggagcgatc ggtgcatcca ccgacgccga tcctgcctgg
    17281 gtggctgaag cgggtggcac gactgcggtg gatgccggtg ccgcgtccag ttggttatac
    17341 gtaaactgcg caggaatcgg atcgctctca ctcagcggcg ccttcgcgga ctgggcctgg
    17401 gtgccctcct ccggcgaatc gagcacaacc tcgatacggc ggtttttttt gcggccctcc
    17461 tcggtcccat tgctggcgac aggacggctc gctccatacc ccacggcaga caacttcgcc
    17521 gaatccatgc cgcccttctc gatcagatac cgcacgatct cggcggcacg ggccttggac
    17581 aactcccaat tggttgggaa tactcctttg agcgacggcc ctatctccat attgtcggag
    17641 tgaccttcca cgcgcacctg ctgagagacc gcagcagtcc tcaaatactc cgccagttgt
    17701 tccagaatgg gtacgccggc aggtttcact ttcacctcac cggattcata ttccaactga
    17761 tctgccagat catcaagatt gatcctggtg cttccatgag cgcgctcgac cctgggtttg
    17821 ctcatcgaat gggactcgct cggcatcgcg acagcgctgt ccgtcctgac gttcatcacc
    17881 ggttggaccg tcgcagccgg aacatccgag tccccgttct catattgcat gacctcaaca
    17941 ggcgaagcgt gtttggagcg aacgcggtcg ccgatcagcg cacgggccgt tgccttggac
    18001 aacaccgcgg tagccgtgtg gggctcggta gaaagaatct tcacttcccc gattttccgc
    18061 tctggaagcc caccgccggt acggaagact tccagatatt cgccggaacg aaggccttcg
    18121 tcttgtccct tgtcgagata gaccaggttt ccctgcgaca cgagcgacat atgtttatcg
    18181 gcctgcagtt caacaatcat ggcttcgatc tcggcatgcc ccgaggcggt ctcaacgact
    18241 tcttctgcta aaggcggtgt aaaccgcatg accggatcgc caggggagag gggaccatag
    18301 gaccgaacaa cctggacgcc aaccagtgcc gcgtctatct gaatgacttt caccaccccc
    18361 actctattga tgatatagcc catgtactgc ttggtcatgg ggtgaaacac tttgcgcgag
    18421 cgccggtata cagtgtagag ctcaccgagc gccgcatcac cgggattctt caatttgaga
    18481 tagagggtat cggtcccgga ccagcccaac atcatccggt tcccggagag ttggttgtcc
    18541 ccggtaatca cattgataac cccgtcaatc ggcatctcct gtcgaatcga gccggcggca
    18601 tacgtgaggg cggcttcccc caggcgaaca ctttgagcgt tctgagcatg aacagggatg
    18661 gccgtcacag ctgcgagact gcaagacagg atgatcaggc tggcgcgcat gggtaatgtc
    18721 ctcatattga tcacacctag gagggttatc tgtagcaacc ttagcaaaga gcaggggctt
    18781 gtcaagaaac gggacggcgc tggacggttt gcttcaggtc tccacgggtg ctatcgtgag
    18841 cctcgcaccg gagtgacgat tcggcaggca tcgatagcag ggcgtctgaa tagcgaggta
    18901 tggcgtggga acagagaagc ggcgcatcaa cactcaaatg ccccgcagat tgaaggttcg
    18961 aaccgccacg cccatggctt cagaatggga tatcagcgtc ggatccccgc cgagtgcgct
    19021 aagaaccccg gccgacaaga tgaggacggg atcctcgaat agccaagcca cccccgacac
    19081 atcgccgaca cttcccaaga agaacgcaag tctccccggc tcatagccac aatccgctgt
    19141 ggcacctaac tgctggacag tattcagttt ctaggacttt cgggactaga ggcctaggtc
    19201 ctttgtgcgc ttgctattaa aaataggcag gggctaagat tccgacctcc taattagccc
    19261 tataagccct caaacccgtt aaggttcgag ccaatggccg gttccgagct cctcttagag
    19321 tacctaagtc gatattttcc gatcctgatg tttgtgttcg tcgcactggc gttcggcgcg
    19381 ggcacattgc tcatcagcta cttcgtccag ccgaagtacc cggaaccgga aaaactctcc
    19441 acttatgagt gcggatctga accgttttcc gatgcccgga tgccgttccc agttcgctac
    19501 tacatctttg ccatgctgtt tgtcattttc gatgtggaag tcatctttct gtatccctgg
    19561 gctattgttt tcaacaagat cggcctgatt gggcttgtag agatgctgat cttcatcgga
    19621 cttttcctcg tcgcctatgt ctacgcgtgg cgaaaaggag ctttggaatg ggattgattc
    19681 agctgggagg ccacgataaa gacgggactc ccgatgtcat tactctcacg gtggaaaaag
    19741 ccgtgaattg ggcccggaag gggtccttgt ggccgatgac cttcgggctg gcttgctgcg
    19801 ccattgaaat gatcgccgcc gtctcgtccc gttacgacat ggatcgatat ggtgccgggg
    19861 tctttcgagc ctccccccgc cagtccgacc tcatgatcgt ggccggaacc gtctgccgcc
    19921 gcatggcccc ggttattcgc aagatttacg accagatgcc ggaaccgaag tacgtgattg
    19981 ccatgggatc gtgcgcaaca tccggaaaca tttacgacag ctacagcgtg gtgcagggag
    20041 tggatcgatt tgtgccggtc gatatttatg tgccggggtg ccccccgact ccggaggcct
    20101 tattcgatgg cattctgaaa ctgcaagagc gcatcatgca aaagcgtgtg ttcctcgcgc
    20161 agccgaaaga agtgaaagac gccctgaagg tctgacgcac ggaaacgaac cgaatgagtc
    20221 aactggccaa acgtatcgag gatacctttc cgggtgcctg cacccaggtg gtggagtggc
    20281 gcggggatgt ttccgtcacc gtcaaacgtg aggccttgca cgacatcggc aagtttttac
    20341 gcgatgaccc ggccatgagg ttcgactaca tcgtccatgt gagttcggtc gattggccgg
    20401 atgacgagga acgtttcgaa gtggtctatg aggtgtattc gattcgaacc cggcagcgca
    20461 ttcgcctgaa aactcgggtt ccggagtcgg attgtattgt ggactccttg accgacatct
    20521 ggaagggcgc tgatttcatg gagcgcgaag tgtacgacat gatgggaatc cggtttcgga
    20581 accatcccga ccttcgtcgc attctgatgc cggacgagta tgaagaaggc taccccctac
    20641 gcaaagactt tccgctccgc ggcaaagggt ggcgcgatac gtttgaattt ttggacgagc
    20701 cgacccggta gggcggccat tttggactac taaacgctca tggcacagtt cgaagatcaa
    20761 cgaactacaa tctataaagt cgacccggaa catccggaag acgcgacgct tccaaccctg
    20821 cgaacggaag agctgctcct caatatgggg ccgcagcacc ccagcacgca cggcgtgctg
    20881 aaagtcatcc tcgagctgga aggcgagcgg ttggtcaaat ctaccccggt gatgggcttt
    20941 ctccaccgcg gtgtagaaaa gctggctgaa gaaggcacgt atcatcagtt cattccgcac
    21001 accgaccgcc tcgattacgt gtgtgcgatg tacaacaact tcgcgtattg ccgcgccgtc
    21061 gaaaagctaa tgaacattac cgtgccggac cgcgccgagt acctgcgcac gatcgtcgcg
    21121 gaaatccagc gcattatcgg ccaccaattc tggctcggca cgcaggccct cgacatcggg
    21181 gcgatgacgg tcttcttcta ttgcttccgg gaccgtgaaa ttctactcga ttggtttgat
    21241 gaactgtgcg gcgctcgcct cacgacgagc tggtatcgta tcggcggcgt cgagcgcgat
    21301 ttcacaccgt ccctgttcgc caagctgaaa caattcctcg actatttccc accgaagatc
    21361 gatgagtacg tgatcttcct ggagaagaac cgcatctggc tggcgcgcac caaaggcgtg
    21421 gccgtgattt cggccgaaga tgcactgagc tttggcttga gcggaccaac gttacgaggc
    21481 tcaggcgttg attacgatct tcgtaaatac gagccatact ccgcctatcc caagtgtgaa
    21541 ttcagcgtcc cggtcggcaa gaacggcgac acctatgacc ggtactggat ccgcgtgcaa
    21601 gagatctacg aaagcgtcaa gatcatccgc cagtgccttg agcagatcca ggacggcccg
    21661 atcatggctg acgtgccgag cgtgactctg cctccgaaag aacgcgtgtt cacgaacctg
    21721 gagtccatga ttcagcagtt caaactcttc tcgcaagggt ttaacgcgcc tcccggagaa
    21781 atctactgcg ggaccgaagc gcataaaggc gaattgggtt tctacatcgt cagcacgggc
    21841 ggcgggaagc cgtaccggct gaaaatccgc gccccgtcat ttatccatat gggcgcattc
    21901 gatcatatgt cgaaaggcta catgatcgcg gacgccgtga cgatcttcgg cacgtacgat
    21961 atcgtcatgg gggaatgcga tcgatgaaga ccgatacagc acaaaggaaa aagtgagcgg
    22021 cgctgattca ttctttcgcc ccatcgcttt tgacttgagg taacttcatg ggtttgaaac
    22081 cagcaaccaa tccagacgtc gaagcggcca cgatcgaact gtcgatcgac ggacggacgg
    22141 tatcggccaa agatggcgtc tcgctgtacg atgtcattgc cagcacgggc aagattattc
    22201 ctgccatgtg ttatcactat acctttgatc ccttcggatc ctgcggtatg tgcctggtca
    22261 tgcaggaagg caagaaggcc ccggtccgtt cctgcactgc caaggcggca gccggtatgg
    22321 tgatccgcac tgacggcgac gacctcttcg cggcacgaaa gaaagccgtc gaaaaacatc
    22381 tctcggttca cccgctggac tgcccggtct gcgacgcaga cgggcattgc gagctccagg
    22441 acatggcctt ccagcatggt gtgaccaatc tcgccaatgc gaagcaaaag ttcattcctg
    22501 aagacacgcg gagtctcgtc ctcgacttca acatgaatcg ctgcatcgct tgcgcggaat
    22561 gcatcaacgt ctgcaaagac gtgctgatga tcgacgcgtt gcaattcatg aagaagggcg
    22621 gattcaacca ggtcgtggcg aaaggggacc aaccccttgc ctgtgaattc tgcggagact
    22681 gcctggctgt ctgtccggtc ggcgcgatca cgaacaaata ttccaagtac ctgtacaagc
    22741 cctggcagat gaaaaaaacc accaccacct gcaactattg tggtgatggc tgccagatgc
    22801 atctcgaaac caaagacacc gaagtcgtcc gcgtgacttc cccgctctcc tggaaaaata
    22861 agtggggcga ccggactgaa actgccaagg gacacggtgg actctgcgtt cgcggccgat
    22921 tcggattcca gtacatcgac agtgaacaac gccttcggca gcctctcgtc cgcacgggcg
    22981 agacgctgac ggaaacgccg tggctcgaca ccatgcaaac tgtgatcgat cgattgtccg
    23041 acattcatcg gaagcacgga ccggagtcga tcgccgggct gattaccgcg cgctgcacga
    23101 acgaagaatt gtatctgttc cagaaactca tgcgcgccgg gctaagaacc aaccagctcg
    23161 acagcagtgc ccgctacggc cacctcaact atgttcatgc cgtacgtcat gccgtcggcg
    23221 tcggccgtcc gctcaacgac tgggaagatt tgaccaaagc caaagccatc ttggtcatcg
    23281 gctcgaatat cacggaaact aacccgctga cggctgtccg catcaaggaa gccatccgcg
    23341 tgtatcactc ccaggtcatc gtcgtcgact ctgccaacac caacatcgcg caactagctt
    23401 cgcatccgct tttggtcaag ccaggcagcg aatcgttctt gatcgatggg ctggtgaaat
    23461 cggtgatcga acaggatctg atcgatgaag ccagcaccac tcggcacccg caggcttttt
    23521 ccgcattgaa gcaagccctg gcacaggtct ccctcgaaca ggtctccgca cagaccggca
    23581 tcgcagtcga gcagatccgg gaaatcgccg ccatttttgc tgaagccccg cgcgccattg
    23641 cgttgtgtgc cgaaggcatc gtccggcagc cgaacggtta ccagaacgtt ctcaagctga
    23701 ttgatctcgc ctgggtgacg ggcaagttag gtcaacccgg atgcggcgtg accacggtca
    23761 cagaagaaat caatgagcag ggtgcagtgg atatgggcgt cgcaccggaa ttcctccccg
    23821 ggcaggcacg attcgatgat ccaggggcaa gggaacgctt cgagaaagct tgggacgtga
    23881 cccttcctgc cagcggaagc ggcgctaatt tcctggaaat tctcgcccgt attcgaaacg
    23941 gccagatcaa ggcgttgtat gtcatcggag agaatcccct ggccactttg cccgcctcca
    24001 tggatgtgaa cgggacgctc gagaagctcc aactgctcat cgttcaagac cccttcctca
    24061 cagagacagg gcaaatggcg catgtcgtcc tcccggctgc cacctacgcg gaaaaggacg
    24121 gcaccttcac caatctggag ggcaaagtgc tccgtgtgcg tcaggcagtt gatcaggtcg
    24181 gggaaagcct gccggactgg catatcatga cggcgctggc caacggtttg ggatatgact
    24241 ggacctatga atcgccgcaa gacgtgcaga atgaaatcat gaagctgtta ccgggctact
    24301 acaatctcgg ccagcctcgc cgcgtctcgc catcggccga tgcctatttg tcgaacggct
    24361 atgaggctga agtcgcgagc cggtatcacc gccagacagc acaatcaacg tccagcgacg
    24421 acacgcagcc ctttacctta ctgaccggac aagtgctcta tcattccggg aaaatgtcca
    24481 cgcatgcatc ggggctcatc aacatcgaac cgaacaatgg gcgaatccgc atgaatccgg
    24541 ccgatgtaga acgcctcggg ctgaacgagc aatccaccat acgcctcacc tctcggcagg
    24601 gttccgtgca agccggcgta aaagccgatc ccgatatcca acccggatcg tgcttcttcc
    24661 ccgagcattt taatgagccg gccgtgaagg acctgatggc cgtagaggtg gacgcagtca
    24721 cgggagttcc gtacttcaaa tccacgcgtg taaagattga aaaggttggt gcgtaagagt
    24781 aagctcgctc gtctttttga tgtcgacaga ggtggtgaga atgagtgtcg gggccttgac
    24841 caagaaaatt cttcaggcgg cgttgttcta cgaaatttgg gacgccatga aggtgacctt
    24901 caagcatatg tttcatcgcc ccatcacctt ccaatacccg cgcgaacaac gcaccatccc
    24961 cgacggtcac cgcggcgcat tagggctgct tcgttatgga gacggccggg accgctgcgt
    25021 gggatgcgac ctttgcgaag cggcatgtcc gtcccgttgt atcaaagtca tcagccagga
    25081 agacccagaa cgtcccttgc agcgctttgc cagcgagttc tacatcgaca tcaccaagtg
    25141 cgtgttttgc ggttattgtg tcgaggcatg ccctgtcaac gcgctcgcga tgaccaagat
    25201 gtacgaatac tcgacccacg acaagcgcac cctcctcttc gacaagaaaa ggctctacga
    25261 ggtcggcgaa cgtcacctcg aggacgccaa aaaatatctg tacgcccata accaagaaaa
    25321 gaattcggac gagagccggg aatatcgtta ttactttccg cagtcggttc tcaaacccac
    25381 acagtcacaa cccaaacatt tgagctgatc gtaccgttat ggccttcctg tttttcgcgt
    25441 atttcgcagt ggtaagtatt gtcgccggca ttctgaccgt cgcgctgaag aatccggtgc
    25501 aatgcggtct ggcgctcctg gccctgctcc ttcacgtctc aggcctcttc gtcatgctga
    25561 acgccgaatt tttgtgggcg gtccaagtga tcgtctacgc cggggccatt ttggtcctct
    25621 atttgttcgt ccttatgttg ctgaacctca aaaccgacga ccgctatttc cacgccaagg
    25681 ctccttatct tctcgcacca gcggccatcg gtttggccta cctcctgttc ttgttggtga
    25741 gatccccgtt cggcggctcc aggggcgatg cctcaccggc ggcgatcttg ctgaacggag
    25801 atgctcatgc catcggcatc aagatgttca gcgagtattt gctgcaattt gaaatcatcg
    25861 gggtgttcct gacaggagcc atcgtcggcg cgatcgtcct cgcaaagacc cccaagtcga
    25921 tcgaaacgag gcgcgacgca tgattccctt gtccgcctat gtggctgtaa gtgccgtgtt
    25981 gtttgcgacg gggttgctcg gcgtcctgat ccgtcgcaat tttattattg tgctgatgtc
    26041 ggtcgaaatc atgctgaacg cggccaccat caacctggtc gccttctccc attacttgga
    26101 agccatgcag ggacaaatcg tggccctctt cattatcgcc attgcagcgg gggaagcggc
    26161 tatcgggctc gccatcatca tcgtggtgtt ccgcggcaag atttcaacca acgtcgacga
    26221 aatgaatttg ttgaagtggt aacgtgtccg actctattga tctgatcgtc aaactgattc
    26281 ctctgttccc gctgatggcc gtcattgtga acggcctctt cgggcatcgg tattcccacg
    26341 acctggctca ccggttcgcg tggggatcgg tcctcatgtc cttcctctgt gtgctggccg
    26401 tcttcacgga gaccctgcgt acaggcgcgg ctcgtgaagt ggtcgcctac aaatggattt
    26461 tcggcggcga tctcacaatc aatctcgcct atttgatcga tcctttgacc tgcatcatgc
    26521 tgctggtcat caccggagtg ggattcctga tccacgtcta ctccgtggga tacatgcacg
    26581 gagaatccgg cttcacgcga ttctttgtgt acatgaacct ctttatggtt tcgatgctcc
    26641 tgttggtgat gggcaataac tacgttgtcc tctttatcgg ctgggaaggc gtcggactct
    26701 gctcctatct gttgatcggg tactactacg acaaggtgtc cgccgccaaa gcggccacca
    26761 aagcctttgt ggtcaatcga atcggtgatg ccggatttct cctcgcaatc ttcctggtgt
    26821 tcgtgaactt ccgaaccctc gactacaccc aggtgatgca gaacgccgca cagctctcgc
    26881 ctgaaatggc cactgcgatc gcactctgcc tcttaatcgg cgcggtcggc aaatcggcgc
    26941 agctcccgct ctacacctgg ctaccagacg ccatggaagg gccgaccccg gtcagtgcgc
    27001 tcatccatgc ggccaccatg gtcacggcag gcgtctatat gatcgttcgc aatcatgcca
    27061 ttttcgatct gtcgcccacc gccatgacga ccgttgcctg gatcggcgga ctcaccgcgc
    27121 tcttcgccgc tacaatcggc ctggtacaga ccgacatcaa gcgcgttctg gcctattcca
    27181 ccgtcagcca gctcgggtac atgttcctcg gttgtggtct cggagcgtat acggccgcgg
    27241 tcttccatct gatgacccat gcgttcttta aagcgctctt gttcttgtcg gccgggtccg
    27301 tcatccacgc attgtccggc gagcaggata tccgcaaaat gggcgcgtta aagtcgaaga
    27361 tcccctggac ccatacactc ttcctgatcg gcacgatagc gattgccgga attccgccct
    27421 tggcgggctt ctggagcaaa gacgaaatca tgggccatgc cttcgtgcat catcactatg
    27481 tgctctacgg catggcagcg gtcggcgcat tcctgacctc gttctatatg ttccgcctga
    27541 cctatctcac gttctatggc acctcgcggc tggatcacca caccgccgaa cacgtgcacg
    27601 aatcccccat ggtgatgatc gggcccctcg tcgtgctggc caccctctca gtcattggcg
    27661 ggttccccgg tgttccgcct gaaaacggct ggttccacca cttcctgcac tcagtcgcag
    27721 gtgtggccgg ggaggagcac gccaccgcgc ccgcgctgat gctcgggctg atgggcacgg
    27781 ccaccgtcat tgcgctcctg ggctggggac tggcgcatta cttttacagc gggccttcct
    27841 ccgcagcaga tgcgctcgcc gcacggatgc cgggcgtgta tacaaccctg ctgaacaagt
    27901 actttgtcga cgaactgtac gaccggctgt tcgtggaacc gaccaagcag atcgggagac
    27961 tttgcgactg gtttgaccgg accatcatcg acggcctcgt ccgatctatc gatcggggga
    28021 cagacgccag ttcggcggcc atcacctgga ccgaaaaaca tgtggtctat gccggcttga
    28081 acatcatcgg ctatgcgaac caccgcttgg cccgctcgtg gcggcggctt cagaccggca
    28141 tggtgcatca gtacgcggct atcattgtgg cgggactttt tattctcgtg cacctcattc
    28201 ttttgatttg gaccggaagc ggttccaacg gctatttatc acgctgacgc agcagaattt
    28261 ctggacacac gatgcttgaa gagttcagtt ttggcttccc gatcctctcg tatctgatct
    28321 tcctcccgct cgccggcgcg gcggtgctct ggttgatcga agacgaagat ctcctcaaga
    28381 cgacgacatt gggtatcacc ctggtggaac tggccctggc ctgcctggtg ctggtgcgct
    28441 tcgtgccgga ttccgctgcg atgcaattcg ccgagcatgc gcgctggatg ccggcgctcg
    28501 gcatcggcta ccatctcgcc gtcgacggca tcagcgtgct ctttgtaggg ctgactgcgt
    28561 ttctcaccgt gctggtcgtc atctattcct gggataccgt acgcaatcag gtccgcctgt
    28621 acttcatggc gctcttggcc ctagagacca ccaccatggg tatctttgcc tcgatcgacc
    28681 tgattctgtt cttcgtcttc tgggaactca tgctcatccc gagctacttc ctgatcaaat
    28741 tgtggggcgg aggagcagag cgccattacg cagccctcaa gtatgtgctc tacacactcc
    28801 tgggcagcgt cttcatgctg gtcggcatcg ccttgctgga catcaactat caccagtggg
    28861 cggtgactca ccacctcgac cacgtctact cattcgacct gctggagtta ctctcggtcc
    28921 cgatcccgct ctcccagcag attttgatct tctggctgat gttcatggga ttcgccttca
    28981 aagcgccggt gttcccattt cacacctggc tccctgatgc cctcgttgaa ggacccatcg
    29041 gtatggccgt catgttagcc gggatgaaat tgggtaccta tggcttcctt cgttttacct
    29101 tcccgttact tcccgatgca tcgaaaagcg aaggcgtcgt ctcgatcgtc atggtcctgg
    29161 ccctcgcggc aattctgtac ggagcggtgg tggcgctcgt tcaatcggac ttcaagcgcc
    29221 tcctcgcatt cagcagcatc agccaccttg ggtttgtggt ggtcgggctc tttgctctga
    29281 atttccaggg cttgcagggg agcctcctga ccatgatcaa cctcggtttc agcaccgccg
    29341 gcctcttctt catggcaggc ttcttatcca ccagacaaca gagttcccaa ttgtcctcct
    29401 tcggaggatt cgcgaaacag gtaccgctgc tggcctcgtt cctcctgttg atcggtatgg
    29461 cctccatcgg actgcctggg accaatggtt ttgtgggtga gtttctgatc ctcctcggcg
    29521 ccttcaaagc caaatggtgg tatggcgccg tggcggtcac cggcgtcatc ttcggagcgg
    29581 cctatttctt gtggtattac gagcgagcca tgctcggccc gctgagcaag aatatctccg
    29641 ccacgatcaa ggacctgcac atgcgggaaa tcaccatcgc actctcactc tcggtgatga
    29701 tcttgtggat cggcctgtac ccgtcaccct tcctgagaat gatgaatgga tcgattcaag
    29761 ccctcgtcga tcgtttggat cgtgccaaag tggcctcggt agacactgtc atccacgtgc
    29821 cggctaagta ggaccatggc tgaatacacg ctgctcatta ttttgttcgc gccgtttgtc
    29881 ggggccctgg ccctgatctt cgtctccaac cgccagctct cgctggtccg cggcattgca
    29941 gccggctcgg cctttgtctc attgatcgct tcagtctatc ttttctatgc ctacgatccg
    30001 ttgaaaggcg gctaccaatt tatccagcgg attgaatggt cacgacagct ggggatctcc
    30061 cttcatctcg gggtggatgg aatcggcacc ccgctggtcc tggcctccgg catcctcctc
    30121 ttcgccggca ttttcgtctc ctggcacatc aaagaccggg tcaaagagtt ttacatctgg
    30181 attctgattc ttgccgcggc gaccatcggc gtattcatgt ccttggattt gttcttcctg
    30241 tatttcttct acgaaatgtc cgtgatcccc atgtatctcc tcttgggcat gtgggggagc
    30301 cacacgaaaa agtatctcga gatgacggac gccgaaggcc tcaagcagcg ggattccgtc
    30361 ggctttatct tcaatttcgg cgcgaacagc aaagaatatg cggccatgaa gctggtcctg
    30421 tttctgtccg ccttcgccgt cgcggcattg atgggtattc tcctgattta caagttctca
    30481 ggactcaaca ccttcgatat tctggtgttg cgcgagcagg cccatttctc ggggcctctg
    30541 gccacgctga tctggctgtt gatctttttc ggctttgcgt cgatcgctcc gatctggccg
    30601 ctgcactcct ggtctcccgt cggccacgcg gccgccccgg cggcaaccag catgttgcac
    30661 gcaggcgtgc tgatgaaatt gggacatttc tcgattatcc gggtcgcctt cgaaatcctg
    30721 cccgagacca cccgcgaact catgcccatt gccgcggtgc tgtgcatctt cagcatcatc
    30781 tacggcgggc tcgtcgccta ttacgcaaag gacaccaaat acgtcatcgg ctattccagc
    30841 tccagccata tgggctacgt gttcctggga atggccgccc tcgactacat cagcctgagc
    30901 ggggccgtca tctatatgtt cgctcacgct atggccaccg ggatgctctt cgccatggcc
    30961 ggctgggtct acgatcaaac ccacacccgc gacattccct cgctgggagg cctctccaac
    31021 cggatgccat ttatttccgc cgccttcgtc atcggctgca tggcctcgat cggaatgccc
    31081 ggcaccgtga actttatcgc tgaagtcatg atcatcgtcg gcagttggaa caaatatccc
    31141 ttccaggtcg tcgttgccgt cctcggtatc gtgctgacca tggcctatct cttcaagatg
    31201 atgcgcggtc tgttctatgg atccatggcg gagaaataca gccattcaca tgatgccgtg
    31261 accgtcgttg atcgcatgcc gctgctgatc atgatcaccg tcagcatcag ttttggaatt
    31321 ttcccggggc acctctactc cgtcgtccga tccggggttg atcccttgat cgctcgtatc
    31381 accaaggtcg tcccggttgc ggagcaccct atgaaccttc cacatgccac ctcgccaacc
    31441 gtacccacat ctgcttcccg tgaagcactt gcgaaggtga ccccgcgatg actttttccc
    31501 ttaccctgtc agtcagcgat ctcctgcttc tgttacccga gctgttcctc actgcctggc
    31561 tgtgcgtcat cctgatcgtc gatttttcgt tcaagcggat cgtccaggaa cagctcgcct
    31621 acctgagcat tcttggatta gtgatcacac tggcctgcct ggcctggttt gacgtgaccg
    31681 gcatcaccgg caccctgttt ggcaagatgt tcgtgctgga ccggatggcg atcttcttca
    31741 aagtgatgat cctcctcgcc acgatcctgg tcattctctt gtcgatcgat tatgtgcacc
    31801 gctttgcgtt cttccgtggc gaatactatt tccttgtcgt gatgtcggca ttaggcatga
    31861 tgttcatgac ctccgccaac gatttgctgt cgctcttcgt caccctggag ttcgcgacat
    31921 tcggctttta tgtcctggtg tcctatctcc gtgacgatat ggcatccaac gaggccggtc
    31981 tcaagttctt catcctggga gtttttgcgg cgggcttgct cgcctatggc atcagcctgg
    32041 tctatggcga aacaggcaag ctcgtcttct cagacatgac tggcgcagcc ccgactaccg
    32101 gactcgtcat cggcttcctg ctcatttttg cggccttggg attcaagatc ggcgcggtcc
    32161 cattccattc atggatcccc gacacatacc acggcgcacc gacgccggtc accgccttcc
    32221 tgtcgatcgc acccaaggta gccgcctttg cgattttgct gcgcctcttc ttagtggcgc
    32281 tggcgacctt caaaccggcc tgggcattgc tcatcgtggc aacctccatc ctctcgatga
    32341 cctacggcaa catcgtggcg atcgcacaac gaaatgtgaa acgtctgtta gcctattccg
    32401 gtatcgcaca agtcggcaac gtgctgattg gtctggcggc ggggaccaag atggggaccg
    32461 attccatctt gttctacctc ctgacctatc tctttgcgaa cctcggagcc tttgccgtta
    32521 tcatggcgat cagcaatgca gtgggcagcg atgaaatcga tgactacaga ggactcaacc
    32581 ggcgctcgcc gtttctggcc tttgctatgc tgattttcct gttgtcgctg gccggagttc
    32641 cccccctcgc cggtttcatc ggcaaattgt atatttttgt cgccgcgatc aaggaggggc
    32701 tctatatcct gatcacggtc ggcctcatca acatcgtcgt gtcgatgtac tactacctga
    32761 tcgtcgtcaa gaagatgtac atcaacgaac cgctcgattc ttctcccatc aagacgacgg
    32821 gtccgttacg ggcggtcatc tatgtcggat tggccggcac actcgtgatc ggtatctatc
    32881 cacagccgtt cctcgactgg gccgtagccg ccacgctgat gttttccaat cttctaggcc
    32941 ctgccgcttc cattcccgta ccagtcgtgc cgttcggcgg gtaagggcca gctccgatcc
    33001 gccttacgaa ttcaatcaaa tcccttcgcg tatcaatagc ttctgctaca atacaatcgc
    33061 ctgatctctg agcggttcgc cgaggctctg cagccttgct cggacgcttc tcaatacctc
    33121 cgcctggttc tcgcatgagt atggacgaac cgaaacccca acaggccgtc aacacagacg
    33181 tggagtccgt ccttcctacc cttccctgga gcgcacgtat tctttcgcgt ctttctattc
    33241 ttgggccgtt ggtcctggca tttcagaacc gctccattga acaacgcgta ctcgctggct
    33301 tcggcctcgt gtttgccggc atcctggtca tttctgccat ttcgtatcgc aatatgacgg
    33361 tgctcatccg aaacggtcat cacgaccaac ggagtcacga gttcatccaa tccttggccg
    33421 cgaccgggga ggccatcgac gatgccgaaa acggccaccg acgattcctg gtcaccgggg
    33481 acgaaagtta cctctccgcc tacaacaccc tgcaagaacg cgcaccggaa tacctcggct
    33541 acctgcggga actcaccgag ccgggcagca ttcagcgcgc acgcgtcacc caactggaac
    33601 agttgatcac ccaacaactg cgggaagagc gagccgccat cgacctgcga aaggagacgg
    33661 ggttcgagtc tgtccggaca ttggccctgg ccggtgtcgc caaaaccgcg ctcgattccg
    33721 tccgtcgact gcaagcgcag atggagcagg acgaaaccaa ggctctcgcg caacgggtca
    33781 tcgaatccac cacgaccact cgttcgagca tcatcctcct gatcatcggc gcccttctgc
    33841 tgttcgtact gctggctgcc gtgtactatc tgattcggca tgacattacg gcacggcggc
    33901 ggatcgccga tgaactccag cgccggggcg aattgctgga ggccgccaat aaagagctgg
    33961 aggcgttcag ctattccgtc tcccacgact tacgagcccc gttacggcat atcgacggct
    34021 acgcctcgct gctggccaag gccgcctcca tctcgcttga cgacaaagcg aagcggtact
    34081 tacagacgat ctctgaatcc gccaccagga tgggacaact catcgacgat ctgttagtgt
    34141 tttcccggat ggggcgacag gaaatgctgc gcgggacggt caacctgaac cagttgatcg
    34201 cctccgtgct gcacgatctg cgacatgact tgcaagatcg gacaatctcg tggacaatcg
    34261 ctcaactccc tgaagtcacg ggggatgcag ccatgttacg acaggtcttt atgaatctcg
    34321 tggccaacgc gatcaagttt accggtacgc agccgcaggc tacgatcgag atcggaagcc
    34381 gtgacaacgg gcagcaggaa gcggtgttat tcgtacgcga caatggggta ggatttgata
    34441 tgcgctacgc gaacaagctg ttcggcgtct ttcagcggct ccaccgagcg gatgagttcg
    34501 aggggacggg tatcggactg gccaatgtca gacgcattat tcaccggcat ggcgggaaga
    34561 cctgggcaga aggtgcgctc ggcgaaggcg cgacctttta cgtcagcctc ccgttggcga
    34621 ggacatcggc atgaccctga ccaaacccat attactggcg gaagacaatc cacgcgatgc
    34681 cgagctcgct ctagcggcga tggaagaaca tcatcttgcg gataaggtca tactctgtca
    34741 cgacggcgcg gaagtattgg attacctcta ctgccgtggc cacttcaaag cccggctcca
    34801 gggcaatccc gcggtggtac tcctcgactt gaagatgccc aaagtcgacg gcctcgaggt
    34861 gctgcgcacc atcaagagcg acgctgccct gaaacccatc cccgtggtga tgctgacatc
    34921 gtccagggaa gagcgggacc tggttgaaag ttatgctttg ggcgccaacg cctatgtggt
    34981 caagcctgtc gagtttcatc agtttctcaa agccgtcaaa gagctcggtg tgttctgggg
    35041 aatgatcaac gaaccgcctc ctgagggggc gagccgcgta accagccgat gacctaaccc
    35101 ccgttcagga ctctgttccc gatacacacg atgactcagc cgttgcgctt catccatctt
    35161 gagagcaacc ccgccgacgc ggcgctgatt gccacgaccc tccgtgacgc cggcattcta
    35221 tgccagctga aacaggcgca gacacgtgag gaatttctgg ccgcacttcg ccaggagggg
    35281 ttcagtctca tcctagccga taccgccgtc cccggcttcg acggagccac cgccctctcc
    35341 ctcgcgcgca ccctgcatcc cgacgtgccg tttctgtttg tctccgatac acagggagaa
    35401 gagtttgcca tcgacatgat gcagcacggc gccaccgatt acatttccaa gcagcgcctg
    35461 gggcgcctcg ttccctccat caaacgaacc ttgcgggaac tcgacgagcg gctggaacgg
    35521 agacgggcgg aggacgccct gcgggtgagc gaaaagcagt ttcgtcaggc ccagaagatg
    35581 gaagccgtcg gacgtctcgc cggaggactt gcccatgatt tcaacaatct gttgacggtc
    35641 atcatgggac acagccaggt gctcttggga gaactgccct caggcaatcc catccgcgcc
    35701 aagatcgaag agatgcaaaa ggccggggag cgagccgcga gtttgattcg ccaactcatg
    35761 gccttcagcc ggaagcagcc gctggaaccg aaaatcctgc ccttgaattc ggtcgtcggc
    35821 aatgtggaaa gcatgctccg gcgactcatc ggtgaagaca ttcaactcgt gatccgccct
    35881 gatcctttta acggccatgt gaaggcagac cccggacaac tggaacaggt cctcatgaat
    35941 ctggtcgtga atgcccggga tgccatgccc aacggcggcc tgctggcgat cgaaacctcg
    36001 caaacggaac tggcacggac cccgatgcat catctccacc cgctgccatt gggacagtat
    36061 gtgaaactca ctgtcaccga taccggatgc ggcatgaatg ccgacgtgct cgcccatcta
    36121 ttcgaaccgt tttttacgac gaaggaagaa cacaagggta ccggactggg gctgtcgacg
    36181 gtcttcggca tcgtgaccac ctgcggaggc gggatcgatg tgtggagtca agtcgggcat
    36241 ggcaccacct ttgacctgta tttcccgcgc gtcaacccgc aagcctctac tgcggatgcc
    36301 accgcaccgc aagcccgcct gcgtcaggga tcggaaacca ttcttctcgt cgaggacgat
    36361 agcggggtac gcaacctggt ccgtcacgaa ctggtcaaaa ccggctatca ggtgatcgag
    36421 gccaagaatg gcgtcgaggc ctgtctcacg gccacacaac agagttacca tgtagacctt
    36481 ctgctcaccg acgtggtgat gccggggatg aacggtcgcg aactggcaca acatctgtcc
    36541 gtcatcaagc cgaacctcag ggtgctgttc atgtcgggct atttggacga tatctccgtg
    36601 aacagcggca tggacccgca tcgaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36721 nnnnnntttc gagaggacta ctcgtacttg ggtgtcttat tgaaagaagg gcggtcactc
    36781 ggcacgaaaa aacatcactc atggcaggga ctattcgcgg cggaaccgac cgacggcagg
    36841 ctccggaagg cccgtgtgct ggaccttgcc ttcgacggat ttacagaaga agccccaccc
    36901 taccgggagg ccgcacaatc gctgatgctg ttcgaccgga cgggaaaact ccgggtccga
    36961 gacagcggga aacgcgcgtt tcaaaccgtg gtggctgaag aaggggaagg cgcaattctt
    37021 gtgatcaaaa cggtggatat cgtctcgctc caccacctcg ccgactgtct ccatcgacag
    37081 atcccgtcga tccaacaggc catggctatg gacggaggcg cctcttcgga tgtgatcgcc
    37141 agcccggatc ttctccatgc cgcccaggag accacgtcgc aagccacctg gcgatcactc
    37201 ctggcgggca atatcggcgt gcacattccg ctgccgactg tcatcggcat cagcccccgg
    37261 acgcacccgc gcaccatgcc cgctccggat gcctcaacat catcacacag ccgatgacca
    37321 acgccgatct accgtccagg cgagacggga aagccggcct cagtccaggc attcatgctg
    37381 cccacgacat tgtagacgtg cgtatagccc aaatcggcca acgtctcggc ggcaatgttg
    37441 ctgcgatggc cggactggca atacacgacg atatggtcgt tcaaggccgc accgatctcc
    37501 cgatgccgcg ccttcatctc gcgaaagtcg atattccgat cggtccccgg aatcatgccg
    37561 gacgcatgct cctcgggcga acgcacatcg accaacagga accccttcgc tccggtcgat
    37621 ggtgccttga gcagaccggc tcgcagttgc tgaaccgtca aggtatagga atgatacgcg
    37681 aaggtgggct gcggccactc caggaggaga ccgcacagca cgcccacgca gagaagacca
    37741 ctcagatatc tcatggtcgc cgctcctttg ttcataccgg gaaggttgac gaacgtccta
    37801 tgataggagc cttatgaaaa gctggtcaag agtggttgcc gtttnnnnnn nnnnnnnnnn
    37861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37921 nnnnnnnnnn nnnnnnnnnn nnnnaacaca tcatcgacga tgagggcccg atctcccgcc
    37981 tgcacgacac cttcaaccaa tctgcccaac ccatggtcct tcggctgctt ccggacaaaa
    38041 aatgtccgcc actcccgacg aggcgtcgcc aaataagcga agtctgaaat cgtcgtcgca
    38101 atcgagatcg ctccgatctc caaccctccc agacaatcga tgtcgagccc cttggtcact
    38161 tcccacgccc gttgcgccat caagtgacgc gcgtgaggaa aggccatcaa ggtgcggcaa
    38221 tcgacataaa acgggctcgt cagacccgag gcgagtttga agccgccctt cggatcccac
    38281 ttgaacgact gagtcttgtg aaacgcggcg atcagatcat cctgcagcat aaccgttttt
    38341 cccatgcggt aatgaaatct tacgtatcca catctaatga gttcaacgtc acgaaaacat
    38401 gaatatagat ggaccacgcc cacccttcag ggtacgatca aatggcccta ttccagagag
    38461 ccgtccctgc gcggcagaca cctagcagga tcctctcatc gcgggcgggc acgtggtcgt
    38521 cggccgcttc ggtggtgcgg tttgttcgat cgcggaagga ggctccgttt cttcggcggc
    38581 cggccgagga cgtgactcaa cggaatgatc cgaaggactc gcgcagcctg accacatccc
    38641 gatcacgctc agaagcagca accacttcaa cacaccaccg cctcgcggtg aaccattgac
    38701 gggttcgatg tcgggcattc tgacatagcc cggcggcaaa gtgaagcaag ggcagatgcg
    38761 ccacacgatg tgtcgacttc tttcgcgact cggccgggat ttcctgatcg tgacttgcct
    38821 gtggacgatc tctgcgacgc acgcagccga acaaccggcc gctcccgccg ctgtgctcgt
    38881 gaagggcttt tcattcagcg ggaacaccgt gatctcccaa gccgagctcg aagccctcac
    38941 gcagccctat gtcgaacagg cactgaccct ccccgatctc gaacgcatcg ccgccgccgt
    39001 cgccgacctc tacaaacagc gggggtatac cctcgcaagc gcgtacattc cccagcagcg
    39061 cattcggttc gggatggtcg agatcagcgt gctcgaaggc cgtctcggcg acatttcggt
    39121 ctccgggaac cggttctact cgacggaatt cattcgcagc ggattcgccg gcgtgatgga
    39181 agcgcgggtg atccggaaca ccgacctgga gcgagccctg ttgttgctga acgactatcc
    39241 ggacctcaag gtctccgcgg cgctcgaacc gggggaattc acgggcagca ccaatgtaca
    39301 ggccagcgtt gaggacaagc ggccgattca cgccacgctc gattacaaca actacggctt
    39361 caacaacatt tcgagaaacc ggttcggagc cggcattgag gtcggcaacg cgctgagaga
    39421 aggggccatt ctgacattca acggaatcgt gggggaccat cccgaacggc tgcaatttat
    39481 caccggcggg tacgccctgc cggtgggcgt gcacggcacg aaggtggtgc tttcaggatc
    39541 cacaggccgg ttcgacgtgg gcgccgagct ggccgctctc cagattcgcg ggaaaataca
    39601 cacctacgac ctgtcgatca cacatccggt ggtgaaaacg cgctggcaat ccctcgtact
    39661 cgataccgga ttcgccgcca aagataatcg actgttcatc aacggcgcgc tcaccggtca
    39721 tgacaccgtc agaatggcca agctcggaat gaactacgat cgagtggacc tctacggccg
    39781 gacctatctg tcggcctacg gttttcatgg cttgggcgaa atcctgggcg gaatggacga
    39841 tcacgcggcc atcagcacca ggccgggtgc cgacaatcgg ttcctcaaag gcacgctcgc
    39901 cgtggggcgt attcagagcc tgggtcacga cgcgctgttg atcctcaaag gaaccggaca
    39961 gatcacgagc ggccccatgg tcgtcatcga acagatgtta ctgggcgggc cggattcggt
    40021 ccggggctat caactcgggg aacgattcgt agacgagggc tacgcggtaa ccgctgaaac
    40081 acggataccg ctcctgccct ccatgctccc ctcgacgcag gccgcgctgt tcttcgatca
    40141 cggcgccggt cacctgcgaa accctcaacc gggagagcgg agatacagca gtctgagcgg
    40201 agccggtgtc ggcctgcaga ctgaactccc gtactattcg atacacctgc gagcagacct
    40261 ggggtttccg gttggtccaa aaccgagcgg gggaaccatt gccggggacc gtagcccgac
    40321 gatgtatctc caggcggcga ccaggttctg atgtacgatt cacccattca taagacggca
    40381 cgggcgccga aaacgggccc cgcttcattt gccttcagcg agccactgct gcggagacat
    40441 cgcatcctct gcgggttgct cgccgcagtc caactactga ttccctccct caccctcgcc
    40501 ctccccacag acggccgggt cgcgggaggt ggcgcgaccc ttcaatccac atcaaaatcg
    40561 atcaccatcc aacaaaccac cgacaaggcc attctgaatt ggaaaagctt ctccatcgcg
    40621 gccgatgaag cggtgcattt cagacaacca tcggccgact ccattgcgct caaccgggtg
    40681 atcggaggag atccctccat cattctgggc cgattgcagg ccaacgggcg catcttcctc
    40741 ctcaacccca acggcattct cttcggcgcc ggcgcgcaga tcaacgtcgg cggcctcctc
    40801 gcgtcaacct tgcacacgcg cgatgaagaa ttcatggccg ggcgttacct ctttgcccaa
    40861 gatccgttga aggggctcaa gaccgtcgtc aaccagggca cgctacaggt ctccgaacac
    40921 ggctttgtca tccttgcggc acctgccgtc tccaacgaag gcatcatcgt cgccaatctg
    40981 ggcacgaccg tattgggatc cggacagcaa ctgacggtcg atcttatggg cgacggtctc
    41041 atcaactatg cgctcaccgg caaagtcctc gaacaggtca cgggtatcga cggaaccgcc
    41101 tcaggctcgg cggtaaagaa cagcgggacc attcatgcgg acggcggaca ggtcattctg
    41161 caggccagcg ccgccggcga cattttctcc tccgtcgtca accagagtgg agtgatacgc
    41221 gcccgcagcc tcacacacga ggatggaatc gtgcgactcg acggagggga cagcggtcag
    41281 gtcacactgg cgggaaccat cgacgcctcc gggcctgcct ccggccaaac aggcggacgg
    41341 gtgtccctgc tcggcgaatc ggtcacgtta acaccgacag cgcgcatcga tgtctccggt
    41401 gacgggggtg gaggcaccgc gctgatcggc gggcagttcc agggacgggg cacagaaccg
    41461 accgcgacca caaccctcgt cgccgaccgt gcatccatct cggcagacgc cctcacacaa
    41521 ggcaccggcg gaacagtcat tgtctgggca gacggccaca ctcagtacgc cggaaccatt
    41581 tcggcacaag gcggagcctc agggggccac ggcggcttcg tggaagtctc aggcaagcag
    41641 gctcttgatt tcaccggccg cgtggacaca accgcaccgg cggggcacac gggaacgctc
    41701 ctgttggacc cgagcaacat caccatctcg tcagcggcca acgccaatat cagcgccgct
    41761 ccgacattca cgggcacgac cgccacatcg acgctgaacg tgaccacgct gcaaaccgcc
    41821 ctcgcaacca acaacgtaat cgtcgacacg acgagcccct tcgcgagcgc cggcaatatc
    41881 acagtctcaa atgccgtgac ctggtccagc gccaactccc tcgatctgcg cgcccacaac
    41941 acgatcaccg tcaatgccgg actctcctcc accggattgg gcatcctccg cctgatcgga
    42001 gaccaggatg gaatcggagg cggcaacgtc gccctcaacg ccgcgatctc agcccgactc
    42061 ggcggcattg agatttccgg cgacggcatc accagcggca cagccggaac gatcaccacg
    42121 accggactgg cgagcggaga cgcgggccac atcacgatca acgcgaccgg
//
LOCUS       DAOQRM010000006        40406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_483_length_40406_cov_14.146291, whole genome shotgun sequence.
ACCESSION   DAOQRM010000006 DAOQRM010000000
VERSION     DAOQRM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 40406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40406
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_483_length_40406_cov_14.146291"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1597)
                     /locus_tag="PKK81_01205"
     CDS             complement(296..1597)
                     /locus_tag="PKK81_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV31511.1"
                     /translation="MSDIAIKADGLSKHYRLGAASHQHNTLRDHLMHRLRGLTRWGGG
                     PPESNPSFWALKDVSFEVKRGEVLGIVGHNGAGKSTLLKILSRITQPTTGTADIYGRV
                     SSLLEVGTGFHSELSGRENIYLNAAMLGMRRGEVRRKFDEIVAFSGVEAFIDTPVKRY
                     SSGMYVRLAFAVAAHLEPEILIVDEVLAVGDASFQQKCLGKMEEVSRSGRTVLIVSHN
                     MTVIEGLCERAILLEKGRVAKIGNTHEVVEGYADAIRGLAGTAIEARHDREGLGEILL
                     TRIEVMDTQRHPVAAAITGRDVVIRMYYRCAAGKEFLNCRVSISVNGRKAQDLFVLST
                     DIVDPKPLTLRGEGYVDFILPELPLTGGAYFFQSYIESNGHAQDWIKNVAPFSVLDAD
                     YYGTGKLCPPGWEANGVLVRYRWEAGETGSTGALPIRARAR"
     gene            complement(1598..2455)
                     /locus_tag="PKK81_01210"
     CDS             complement(1598..2455)
                     /locus_tag="PKK81_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV31512.1"
                     /translation="MSALTVDQRPSSTLPHVRIQPARGLLDLDLAALWQYRELIYILV
                     WRDVTVRYKQTLLGVAWSMFQPVATMLIFTVVFSVMGKIPSDGFPYPVFLYAGLLPWF
                     YVSQALSRGGTSLVGEAPLISKVYFPRLILPLSATIAPLVDLALAFVAFLGLMAWFGI
                     IPTWRILSLPLFAALAATITLAAGLWISALYVKYRDVGHILPMFIQLWMFVSPILYPV
                     SKVPESWRALYALNPVVSVVEGFRWALIPGFQVDFMMFLPSLGVVFVVFVGGLIYFRS
                     MERTFADVI"
     gene            complement(2832..3377)
                     /locus_tag="PKK81_01215"
     CDS             complement(2832..3377)
                     /locus_tag="PKK81_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31513.1"
                     /translation="MNIRNRVMMKQASRLALILFLGLSSACVSVQVDPLTSKAFEPRP
                     GSEDVTTLQREPNHGHVQIARIIATSEYASEDTLRDRILARAKELGADAVVLGDADVR
                     RLADRGPTFQSTMGTAVPGATSGNSYRSGYWNPFRMDAWSFTQGAGGGQGWMLHMSGL
                     AIRYVSADELRAAPKPVPPAP"
     gene            complement(3546..3905)
                     /locus_tag="PKK81_01220"
     CDS             complement(3546..3905)
                     /locus_tag="PKK81_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HNV31514.1"
                     /translation="MIRSLQPCPSSPNCVSTQASDHGHGIVPYRYQKTLAGAKETLKT
                     IVANLPRAKLVEEDEAYLHYEFTSLLLRFVDDVEFVFDDATKTIHFRSASRTGYSDLG
                     VNRRRMEEIRALAEGRL"
     gene            complement(4084..5268)
                     /locus_tag="PKK81_01225"
     CDS             complement(4084..5268)
                     /locus_tag="PKK81_01225"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNV31515.1"
                     /translation="MVSSNNMDATSLWPAVAALLLAVGTATLLPDIGHLRTTSAARYS
                     CIDGLRGYLAFAVFLSHSSVWYFYLRSGTWDVPPSNFYTHLGQSSVTLFFMITGFLFW
                     SKLLDGRVQPVDWSRLYLSRIFRLVPLYLLMVIGVVSIALYRAGFRLQESMTSFALHL
                     ASWLTFTIPGIAPVNGFAETIPLAGAVWSLPYEWLFYAFLPLGSWCLRAATPTSWLLF
                     SAAAVVTLTTVMPDVRGPVLYAFGGGIAAASLARVDAIRTRLAHRVWGLVVVACLGIT
                     MWLFPTAYTFPALMLLTGAFTIIACGNSIYGILEWPTSVLLSEMGYSLYLLHSLLLFA
                     AYRLVLGEWASTLSAAEHWSIVLGLVPVLILLCFATFRLIEQPAMAAVPRCHAWLVAH
                     LN"
     gene            5520..6695
                     /locus_tag="PKK81_01230"
     CDS             5520..6695
                     /locus_tag="PKK81_01230"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNV31516.1"
                     /translation="MAGFPIEVATRIKTLPPYLFAAIDKMKQEALARGVDIINLGIGD
                     PDLPTPTPIIDSLAQAAKNPKHHQYPSYEGMLSFRKAVADWYKRRFNVALDPADEVLT
                     LIGSKEGIGHVHLAFIDPGDIVLVPSPGYPVYPVGTSFCGGVSHIMPLTKANGFLPDL
                     NAIPKEVAKKAKLMWLNSPNNPTSVIMSKDYFKRVVEFAQDNQVIVCHDAAYSEIYYD
                     GKRPPSFLEVDGAKDVGVEFHSLSKTYNMTGWRIGFAVGNKGVLAGLGRVKSQLDSGV
                     FEAVQAAGITALGLDDSVTDGLRKIYQERRDTLVPGLKKLGLEVDPPPAAFYIWVTVP
                     KGYTSASFTAHLLEKAGIVTTPGNGFGAPGEGYIRMTVCTTKERLAEAVERIKKAGF"
     gene            6783..7346
                     /gene="folK"
                     /locus_tag="PKK81_01235"
     CDS             6783..7346
                     /gene="folK"
                     /locus_tag="PKK81_01235"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNV31517.1"
                     /translation="MATAFIGFGSNLGNRIDFCDRAVTLLGLLPHSRLEAVSSLYETE
                     PLEDGAAPGPSWFLNGVVQIETNIAPKSLLEICREIERALGRDQDNRKGPRTLDLDVL
                     LYDDCILAEPALTVPHPRLHQRRFVLTPLVELDPERRHPVLGQNLRDLLSQLTDPSIV
                     RLLEPQPNSRYGSRPTCSAPPTAGPQA"
     gene            7343..8200
                     /gene="panC"
                     /locus_tag="PKK81_01240"
     CDS             7343..8200
                     /gene="panC"
                     /locus_tag="PKK81_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNV31518.1"
                     /translation="MKIIRATPAMADWSRRLHREGITIGFVPTMGALHEGHRALIRAA
                     RLACDAVVVSIFVNPTQFGPTEDLSKYPRQLRRDQALCRKEGVDVIFAPTVRTMYPTG
                     AQTVVTVPGIARRWEGEARPHHFQGVATVVTKLLSLVRPDKTWFGQKDYQQAALVQQL
                     VKDLNLPGLPIVHPTVREADGLALSSRNVYLSSSERQAAPVLSRALQAGVAAIRAGER
                     NGTRIRRHMLRVVATEPLATVEYLAVCDPATLEPLSLLNRQAVLLGAIRIGTVRLLDN
                     VLTSFRRKP"
     gene            complement(8286..8729)
                     /locus_tag="PKK81_01245"
     CDS             complement(8286..8729)
                     /locus_tag="PKK81_01245"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV31519.1"
                     /translation="MEKTLNRLSIFQVRCQLCTARFQARRPGNRQTSQEFDRREYRRL
                     RANFAASLILDQPAVGGVITDISMGGCTLQASSSLPRGTFVKLIVHAPAGQPDIKVDA
                     AMICSIQPLSIGVKFLEFEPDDKQRMGQLVLELLAHQQAPPRVSA"
     gene            complement(8797..9225)
                     /locus_tag="PKK81_01250"
     CDS             complement(8797..9225)
                     /locus_tag="PKK81_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNV31520.1"
                     /translation="MMDPQAMVEEFHRKFDIAVSDRPSLPEEATRQLRVRLIQEEFEE
                     LQEAMVAQDLPGVAKELADLLYVVYGTAVSYGLDMDPVFREVHRSNLSKVGGYKRADG
                     KWVKPPTYSPAQVEPLLAAQSSGSLAGRSQVDTRMQESES"
     gene            complement(9222..10637)
                     /gene="rmuC"
                     /locus_tag="PKK81_01255"
     CDS             complement(9222..10637)
                     /gene="rmuC"
                     /locus_tag="PKK81_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNV31521.1"
                     /translation="MIDLTSPTFLVGTAVGLAVGVLVAGWWVIVRVQSRHHARLLESG
                     ERAQRADAVAASLQQRVDDQQSEMGQLRDALAESQQGRTKAETRMEEVARSLEDQKAL
                     LAQARQELHDSFEALSGQALKQNNEAFLDLARTSFATLQAEAKGELSQRQQAIGELVK
                     PLEESLQRYQEQLQQAEQVRQREYGGLDQQLRFMAESHQRLQQETGNLVKALRAPSVR
                     GRWGEMTLRRVAELAGMVAHCDFVEQDTIGSLEGLIRPDMVVYLPGDRQIVVDAKTVL
                     AAYLDAYEAEDDRQRQAHLLRHAGQVRARMDALSLKAYWTQFGQTPEFVVLFLPGEQF
                     LGAALEQDPTLIEDGFAQGVVLATPTTLMALLRAVAYGWKQEQLNEHAEQAGRLGKEL
                     YERMAVLTDHLNEIGQALGKSVGAYNKAVGSLESRVLPAARKFKDLGISSDKDIALLE
                     ASGIEPRTGVAFAAESRGTLG"
     gene            complement(10733..13363)
                     /locus_tag="PKK81_01260"
     CDS             complement(10733..13363)
                     /locus_tag="PKK81_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV31522.1"
                     /translation="MADPFLLSLYESCAPFLVGGKAAGLAKVLTAGLPVPKGLCVTTA
                     VYQHGLDQAGVDAVALWTKLPHLSEGQRAQELARIQRLLLEQPWPTGFPADLETRLTG
                     LAGDSSTRWAVRSSATNEDAADMSAAGLYRTTLGVPLAAVLQSIRDCWISLWDERVFR
                     YFLRSGADRPCPAMAVVIQPMLSAQAAGVAYSIHPVTGRTSQVSIDAVPGLASSLVNG
                     VVTPDQYVVEVYDDDHRPFRVRRRMLARKRNKLTATPGGVREESIPEPEQRQACLTDK
                     QLFELARLSKRIEVAFRQPVDLEWVLDVERLWIVQARPITAVRPWPSLTNDECEWTRA
                     NFKETLPELPSPLGLSFLERFMDAYIITPYRRLGCTVPEGLSSVRVFHGRPYLNVTLF
                     YTLVMQLHGNPAFLTEQMGGEPLMFTPSVRPLGALALLRAGVGMLREWRKAAKQGPKN
                     FSAMKAMAQRYRYDRIQNLSVQELAAILDSIGRWLDDHEVTFAIAGGVAQSLQAMGTV
                     LPGWLGSDWRELLNGALQGQGTVISASQIVRLAELVVAAQQEETVRQWFFSEEWAACG
                     YRDAIQGTEFLRLFDQYLAEYGHRAVGESDIMSPRIADQPDAVLALLRAQVRAGVTAP
                     PQEVLSRQAQRREQALSEIARRFGWRRHRWLVFRWWYRRLSRFCALREENRHHLMYYS
                     TAARHLLLRLGELMVARGSFAVREDVFYLTLDERIALTDGASRDWQSLVRRRREERLQ
                     YEALQVPDTIRDWEAVVEQGAQSSDQGQGGVLRGIAISAGVAKGPVRLVRSTADWARV
                     RAGDILVVPVIDPGMAPLFGMAAGLIAEMGGTLSHGAIIAREYGLPVLVNVPYATSLL
                     RENEQVEIASSTGRISRLVS"
     gene            complement(13379..14719)
                     /locus_tag="PKK81_01265"
     CDS             complement(13379..14719)
                     /locus_tag="PKK81_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNV31523.1"
                     /translation="MIQVGVSQLRARALFTTLFRAGLQAVDPYEAVCRQVRFRRGQLT
                     IGSERYPLSQHQRLVVVGAGKASGRMAQALERQLGARIDTGLVVVKYGHGAPTKKIRI
                     LEAGHPVPDEAGLQASRAMMALIGTLKSDDLLIVLLSGGASSLLPSPAPGVTLKAKQL
                     TTKLLLRSGATIHEMNAVRKHLSCVKGGQLAAATSARVASVILSDVIGNDLGTIGSGP
                     TAPDPTTFRDAWRILERYEVARKVPASVRRRLEAGMKKTLPETPKAGAVLFRRVDNLL
                     IGDNQAAVDAVVKAAKGKRLQTLVLSTTVTGEARELAKFFGAIAREIATHGRPLARPC
                     CVIAGGEPTVTIRGQGKGGRAQEFALAAALEIAGLSDVWVAGFATDGTDGPTSVAGAV
                     VDGETTARARRAKLDPPGALQGNDAYPFFKKLRSHIVTGPTGTNVNDLYLLLAL"
     gene            complement(14725..15804)
                     /locus_tag="PKK81_01270"
     CDS             complement(14725..15804)
                     /locus_tag="PKK81_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNV31524.1"
                     /translation="MWNKERGVQAFLACVTMLFGLSSAMPVLSADSSSAALTGAKAEE
                     GRNAQESDQVPVAPPGESELVPELEDRLVILPEIKREGERFFLSSFKLPDKLTFAGQA
                     IPLDNWQVRERIEYEFYQFLEDQGESIILAKRTGRCFAPAEKQLAEAGLPDDLKYMLL
                     VESKCIAAAYSRAKASGPWQFINSTGRRYKLHNERWLDERRNLEMSTEAAIKYLRYLR
                     DLYQGDWFLAMASYNAGEDRVRKLIKEQKINDYWRMHGPRETMRYVARIIAAKEIYSQ
                     PEKYLGLTKKDLYPPLETETVTVTIKEPQRRLTAIAEEYGTYFLELKMLNPEFTKDYL
                     PKGTYQLKVPRQTCPNRCFKQEKLP"
     gene            15899..16597
                     /locus_tag="PKK81_01275"
     CDS             15899..16597
                     /locus_tag="PKK81_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNV31525.1"
                     /translation="MSALTMLNNEIVACTCCPRLVAYREAVARDKRRQFREWSYWGKP
                     VPGFGDSNARLYILGLAPAAHGGNRTGRVFTGDRSGDWLYEALHRFGFANQATSVHAG
                     DGLTLTDCYIGATVRCAPPANKPTPDEFTACRPFVLRELRLLKRMRVIVTLGKIAFDH
                     YLKASRELGLGTPGLRPQFGHGVVYDLPWGVTLIGSYHPSQQNTFTGKLTRPMFHRIF
                     LKAQQKLAEAPLRD"
     gene            complement(16611..17258)
                     /gene="fsa"
                     /locus_tag="PKK81_01280"
     CDS             complement(16611..17258)
                     /gene="fsa"
                     /locus_tag="PKK81_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNV31526.1"
                     /translation="MQIYLDTANVKEIQEGANLGLIDGVTTNPSLVAKEGRSFKEMLL
                     EICKMVDGPISAEVVGVESEAMIKEGRDLAKVHKNIVVKVPLIPEGLRATKKLAAEGI
                     RVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDVSSDGMALIRQIVTIYKNYDYKTQV
                     LVASVRHPQHVVEAALAGGHICTMPYAVFQQLVKHPLTDSGLKKFLADWDAMGKK"
     gene            17457..17759
                     /locus_tag="PKK81_01285"
     CDS             17457..17759
                     /locus_tag="PKK81_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31527.1"
                     /translation="MRKSAWLIAIALLSGACSHNPYLDASLTRYQGKDRAWIEKELGA
                     PDAKTSRFFGGEKWTYNRIAGGKAGPPLFNFKANECQLILFFNKDEMVSDSSYSGC"
     gene            complement(17756..18016)
                     /locus_tag="PKK81_01290"
     CDS             complement(17756..18016)
                     /locus_tag="PKK81_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNV31528.1"
                     /translation="MYRLLLVSGLLVLLYYLLRRAIRKMKEDGGAVSLQEEQAAGNQM
                     IQDPVCRVFIPRGNAVREEIGGQTYYFCSRSCADTFQKQLSS"
     gene            complement(18709..19512)
                     /gene="dapB"
                     /locus_tag="PKK81_01295"
     CDS             complement(18709..19512)
                     /gene="dapB"
                     /locus_tag="PKK81_01295"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNV31529.1"
                     /translation="MIKVVITGAAGRMGSRLVSLVKDSAFLTLAGAVEGKGHHAVGED
                     SGEVAGCGRTGVPIVDDLSSLMERGEVVVDFTTPAATLGHLKIVAQHRRGIVVGTTGF
                     SSSELDELRSVSKQIPCVFSPNMSVGVNVIYKVIAEMAKTLGEDYDIEVIEAHHRLKK
                     DAPSGTALKMAEVLARAVNRDLGQVGVYARKGLIGERKRGEIGIQTIRAGDIVGDHTV
                     MFGTMGERIELTHRASSRDTFARGALRAARWVVKQPPGLYDMLDVLSLK"
     gene            complement(19542..20414)
                     /gene="dapA"
                     /locus_tag="PKK81_01300"
     CDS             complement(19542..20414)
                     /gene="dapA"
                     /locus_tag="PKK81_01300"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNV31530.1"
                     /translation="MFTGSLVAIVTPFKNGKLDEKSLGDLIEWQITSGTQGIVPCGTT
                     GESATLTHAEHDRVVAFTVEVARRRVPVIAGTGSNSTQEAIALTKHAKAAGAGGALLI
                     TPYYNKPTQEGLFLHYKAVAEAVDLPLVLYNIPGRTGVNMMPSTISRLTVCPTIVAIK
                     EGSGSVQQASEIIQLCGERLTVLAGDDALTLPMMAVGGKGVITVTANLVPSDMAELVN
                     AFLAGRVDDARAMHYRLYPLFTALFYETNPIPVKEALHMMGKIDREMRLPLCPMGTDN
                     REKLLHVMKEAGLV"
     gene            complement(20451..21713)
                     /gene="lysA"
                     /locus_tag="PKK81_01305"
     CDS             complement(20451..21713)
                     /gene="lysA"
                     /locus_tag="PKK81_01305"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNV31531.1"
                     /translation="MNDFQYREGELYCEDVPLSRIAKEVGTPCYVYSHHTLVRHFRVY
                     DSAFQNIPHIVAFAMKANSNLAVLRLMAKEGSGVDIVSGGELFRALKAGVSPAKIVFA
                     GVGKKAEEIRDALKADILMFNVESSAELQAINDVAASMGVKARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRAIEEFKAAATFSHIEVVGLHAHIGSQLTQVAPFVESLKKVL
                     AMVQTLAEQGIPIRYLNIGGGLGITYSDETPPEPKDLAAAIFPLVRDLKCILIMEPGR
                     VIVGNAGVLLTKVLYTKDGETKRFLIVDAAMNDLIRPSLYDAHHDIRPVYENVARAAK
                     QTVDVVGPVCESGDFLAKDRVMPEMNAGDLMAVMSAGAYGFVMSSNYNSRPRVPEVLV
                     HEGQIHIIRARESYDDLVHGEQIPAFLS"
     gene            complement(22115..23569)
                     /gene="argH"
                     /locus_tag="PKK81_01310"
     CDS             complement(22115..23569)
                     /gene="argH"
                     /locus_tag="PKK81_01310"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNV31532.1"
                     /translation="MAKPQSAGARARKSVTAPQRSRRALPKGKAWGGRFAEQTDRLVE
                     QFTSSLACDRRLYPYDIQGSIAHCRTLERAGVLTARESAQLVRGLQFVKVELDGGRFP
                     FSPQDEDIHMAIERRLTELIGPLGGKLHTGRSRNDQVALDLRLFLRDVLSTLMGQLQE
                     FRRVLVGQARAHLDVVMPGYTHLQRAQPVLLAHHFLAYVEMFDRDRGRLHDCRQRLDV
                     MPLGSGALAGSNYPVDRRYTAALLEFPAVTQNSLDAVSDRDAVVETLSALSLIMMHLS
                     RLSEELILWASQEFRYVDLPDTFCTGSSMMPQKKNPDVPELVRGKTGRVYGHLMGTLT
                     LLKGLPLSYNRDLQEDKEALFDAVDTTGQSLALCTELMRRLVVNKTVLAEAAEGGGML
                     ATELADYLVTKGVPFREAHSITGQIVRFSLEQQRPLQQLTLKDLRGFSAHFGEDVLSC
                     LTVRGAIDRKGQIGGTARRRVEARIKELEKALKG"
     gene            complement(23569..24786)
                     /locus_tag="PKK81_01315"
     CDS             complement(23569..24786)
                     /locus_tag="PKK81_01315"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNV31533.1"
                     /translation="MSRSSYKKVVLAYSGGLDTSVILKWLEEVYGCEVIAFCADLGQG
                     EDLKAIKKKAQSLGVKKVYVEDLREVFVKDHVFPMLRGNAIYEGSYLLGTSIARPLIA
                     RRQIEIAADEGAEAVCHGATGKGNDQVRFELTYMALHPQIKIIAPWREWTMRSRRELI
                     EYAEKHGIPVTATKAKPYSMDMNLFHTSYEGGILEDPWKAPPEEIFVMSVSPERAPNK
                     AREVEIDYVAGNPVAVDGKKMSPAALLAHLNKLGGEHGVGRVDLVENRYVGMKSRGVY
                     ETPGGTILHAAHRGLESLTMDREVLHLRDSLIPRYAELIYYGYWYAPEREMLQVALDE
                     AQRDVTGTVRVKLYKGTCTVVGRKSPRSLYRLDMATFEEDDVYRQKDAEGFIRLSALR
                     LAIRAQRKKRSTR"
     gene            complement(24802..25782)
                     /gene="argF"
                     /locus_tag="PKK81_01320"
     CDS             complement(24802..25782)
                     /gene="argF"
                     /locus_tag="PKK81_01320"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNV31534.1"
                     /translation="MSRRPHRSSSRAGLGKDFLELLSIPVAELTGLLRLAAQLKVKQR
                     RGVSHPLLQGRMLGLLFQKPSTRTRVSFEAGMNQLGGQAMVLPMGDIQLSRGESVADT
                     AHVLSRYLDAIVLRTFDHAIAEEWAREASIPVINGLTDLNHPCQALSDLLTIQEKKRR
                     LKGIKIAYVGDGNNVTNSLIEAAAKMGMTIAVGCPLGYQPDRGIVDRAREEARQTGAV
                     IEIGADPFVAVKDADVVYTDVWISMGQEREQAKRLKILAPYQLNARLLKCAKPDALVM
                     HCLPAHRGEEISAEVLDGPQSVVFDQAENRLHMQKAILVRLLGKKPVRST"
     gene            complement(25799..27007)
                     /locus_tag="PKK81_01325"
     CDS             complement(25799..27007)
                     /locus_tag="PKK81_01325"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HNV31535.1"
                     /translation="MPTGELRLNAEQYLMNTYTRQPISIVRGRGSKVYDLEGREYIDF
                     VAGIAVNLLGHGHPDLVLAIQKQVQHLIHTSNLYYTEPQVRLAQTLVEHSFAQKVFFC
                     NSGAEANEAAIKLARKYSYDKYGADRYEIVTMTNSFHGRTMATLTATGQEKVQKGFAP
                     LMPGFSHVTFNDLSEVERAITSKTAAVLLEPIQAEGGVHVADRGYMQGLRDLCRERDV
                     LLIFDEVQTGMGRTGTLFCYEQFGMQPDIMTLAKGLGGGVPIGACLATETVAQAFSPG
                     THASTFGGNPLACAAALAVLRVLLEGKILDQGRRMGETLAKGLAVLKDRHRCVKDVRG
                     LGLLQGVELDIDGKAVVADCLARGLLINCTGDRVLRFVPPLIITEREIDRLLVALAQV
                     LSQRTTSSHH"
     gene            27180..27824
                     /locus_tag="PKK81_01330"
     CDS             27180..27824
                     /locus_tag="PKK81_01330"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HNV31536.1"
                     /translation="MKSCSQCRQENRDDARFCHQCGGAFAVESRETVVEADSAPSPQT
                     DAELWKAFIGPNADRYLETFKKFTGPSGPKFALTWHWPAFVFEPFLWFLYRKMYVYAL
                     IYAIGPAMAFYITQDLSADIIWRVIAGASANYIYFWHAKEQLAKIKGERATGGEARQQ
                     MLGELGGVQPYVVWVGVGLLVLKIGLVVAMFKDGPPDGSKNPPAKPHPASVTHV"
     gene            complement(27887..28354)
                     /locus_tag="PKK81_01335"
     CDS             complement(27887..28354)
                     /locus_tag="PKK81_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31537.1"
                     /translation="MAVWQFQIELVPKAVAEANSLRTGMVLTEEQRESVVWWRDQRLP
                     LTLLRDVEKMLPSGKTWSKDLEVLGSLETSCLTILREGESLVEVQFRFDLRETTIELL
                     ETAVQFSRDLQCWLISEGGKVIEPGLSALKDEIRESTAFQFVKDPLSFLSRLK"
     gene            complement(28420..29250)
                     /locus_tag="PKK81_01340"
     CDS             complement(28420..29250)
                     /locus_tag="PKK81_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31538.1"
                     /translation="MTERSPSDWTPLQEWWRAMGRRLQVEDRVEAFFRRALGVSQALA
                     GVAQAGGEVEYVLFVLVRYWIPVVLPPPGRERASKGDPDYWLESEQILKAAAVRLREL
                     KPLIELLTVANPLSTGGPDHSPARSPVVEVELANMLQGIAESAGSYGGPDYTSVIKTF
                     DPVPLRQTQPFKHNKKNSAELWVVFLLREHFRSVGLGKDRYWPLVAGLCAAAGITNPN
                     GQPYGPDELKSWWQKNWPRTYTQLEQTQAAPDLSGAAYQQDFDWFVSWIEWQRQRLSE
                     "
     gene            29433..>29551
                     /locus_tag="PKK81_01345"
     CDS             29433..>29551
                     /locus_tag="PKK81_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNV31539.1"
                     /translation="MSKLVLIRHGESQWNLENRFTGWVDVPLSPKGIEEAKAAG"
     assembly_gap    29552..29651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29652..29915
                     /locus_tag="PKK81_01350"
     CDS             <29652..29915
                     /locus_tag="PKK81_01350"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNV31540.1"
                     /translation="YDVRPPGGESLKDTAERVLPYYDSRIKPYVLKGETILIAAHGNS
                     LRALVMQLEQLTREQVLELNIPTGAPLLYELDNNGKVLSHRYL"
     gene            complement(29944..30438)
                     /locus_tag="PKK81_01355"
     CDS             complement(29944..30438)
                     /locus_tag="PKK81_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31541.1"
                     /translation="MPKGAMTPAEVADALYKLIPRRVSVELLSEYGIEGQEEHEETMT
                     RELLSFTLYWVHAAVNAHIPRKYREVLFQRVLELIQADWAATFKLESVKWEDYLVEME
                     ERRALYAPVGDYEGGAMAASEEISDLLENQCLIQPEDRPKLLVLLPDLVPLDKYQELL
                     SQCV"
     gene            30585..31136
                     /locus_tag="PKK81_01360"
     CDS             30585..31136
                     /locus_tag="PKK81_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31542.1"
                     /translation="MTPMRPVRALFIGLAFLGILWAAPTTAAEVQEPRWGFSTDLGLW
                     SGTTNDTTFALGFGLDYYMDQNFSFGGMALFTPVGDLTQIGIAGVAKYHLRLNSGFNL
                     VPFAGLGILHADLNRGSGPTKVDRNDTSHFIPLGMSLEYQVGPKIAFSTTLMVNLHHI
                     TLSAPVPDDNTSVALLFGIRWGP"
     assembly_gap    31208..31307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31308..32326)
                     /locus_tag="PKK81_01365"
     CDS             complement(<31308..32326)
                     /locus_tag="PKK81_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNV31543.1"
                     /translation="MRRLRVLVLMHKDLVPPDQLNGQDLEGAAWKTEYDVVSTLRKLG
                     HDVQPLGVKSDLEVIRAAVEDWKPHIAFNLLEEFDGRAVYDQNVVSYLELMRIPYTGC
                     NPRGLMLARDKALAKQVMSYHRIPYPEFMVVPLHRMVRRPKYLPFPLIVKSISEEASL
                     GISQASIVDDDEKLRERVAFIHDNVGTGALIERYIEGRELYVGVMGNAHLQVFPVWEL
                     VMDKMPDEARRIATERVKWSRKYQDKYGICSCEARNLPDGVVDHIQHLAKRVYRALGL
                     SGYARIDMRMDKDGNVYVLEANPNPQIATGEDFADSAEKADLAYKDLLQELLHVGLRW
                     RPAQAA"
     gene            complement(32323..>32505)
                     /locus_tag="PKK81_01370"
     CDS             complement(32323..>32505)
                     /locus_tag="PKK81_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31544.1"
                     /translation="EWTGEYQYTIDQVLEGMIERCQASKLHLTQHKEQAKLDFAILLT
                     VQTMNYLHSGRHKVAL"
     assembly_gap    32506..32605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32606..33234)
                     /locus_tag="PKK81_01375"
     CDS             complement(<32606..33234)
                     /locus_tag="PKK81_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HNV31545.1"
                     /translation="MGRTKNHRGQGIGATDPVPVWAEWTDEQLLDLRMCDLDLRLEGT
                     FYQEPIAQLARELETRRLTFRPHFWISDEWFTPDGVPGIAVPFYLAHPRLAKLEASQM
                     LEVEGGTRDWCMRILRHEAGHAIENAYLLRRRRRRQKLFGRSSQPYPEYYTPRPYSRS
                     FVRHLDVWYAQSHPDEDFAETFAVWLDPHSLWKERYRGWPVMKKLDFMDR"
     gene            complement(33417..33884)
                     /locus_tag="PKK81_01380"
     CDS             complement(33417..33884)
                     /locus_tag="PKK81_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HNV31546.1"
                     /translation="MTSILFVCSGNIFRSLVAEYALKAQLGSRVGYLIGSAGIEALPQ
                     EIHPVVLAGLREKGVDPSRHRQRKLTRDLIEETAVVIAMGRNHQAWIRGEFGREVPLF
                     NHVCYGIDQPILDVHEIIPDWREQPDQSRDYLQFVIDHIWGGVPHLLNREPAL"
     gene            33963..34838
                     /gene="ttcA"
                     /locus_tag="PKK81_01385"
     CDS             33963..34838
                     /gene="ttcA"
                     /locus_tag="PKK81_01385"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNV31547.1"
                     /translation="MQSTTTPPSPGTAQPTRHSNEIERVHTRLCRLVGQAIADYRMIE
                     EGDKVMVCLSGGKDSYGLLEILLSIRSRAPIHFDIIAVNLDQKQPGFPAHVLPEYLTR
                     RGVPFHIETRDTYSVVKRLIPEGQTTCSLCSRLRRGHLYRLATELGATKIALGHHRND
                     ILETLLLNLLFTGKMKAMPPKLRSKSGRHVVIRPLAYVKEADLARYAALLQFPIIPCD
                     LCGSQEDLKRKQVKTLLQDWDQRFPGSNDSMFAALGNIAPSLLLDRTLFDFSSLKADA
                     SPTEPAASDSEDDLL"
     gene            35706..36143
                     /locus_tag="PKK81_01390"
     CDS             35706..36143
                     /locus_tag="PKK81_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31548.1"
                     /translation="MKRSTPSRRPAAEPPLWSIAAAAGVIILTPMLLYSLAPEGPIRE
                     GDTVFSSGAHKVSLFEPDRYRQAGYEATCMLDPKDPLIVTHSPSEDESGEVIIAQVQG
                     KSTIEWPFCPPQAELLVKRHHITQQPSILQDIRDGLLRLFKPS"
     gene            complement(36168..36713)
                     /gene="msrA"
                     /locus_tag="PKK81_01395"
     CDS             complement(36168..36713)
                     /gene="msrA"
                     /locus_tag="PKK81_01395"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNV31549.1"
                     /translation="MGQETTDIATLAGGCFWCLEAVYDQVNGVRSVESGYIGGQVDAP
                     TYEQVCGGHTGHAEAVRITFDPRLVTYRDLLNVFFVIHDPTTRNRQGNDIGTQYRSGI
                     FYHSPEQQQIAQEVIAAVTRERVYPNPIVTEVVPATRWYEAEAYHQEYFARNPFEGYC
                     AYVVGPKVAKFRQHYASLLKA"
     gene            complement(36746..38257)
                     /gene="zwf"
                     /locus_tag="PKK81_01400"
     CDS             complement(36746..38257)
                     /gene="zwf"
                     /locus_tag="PKK81_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNV31550.1"
                     /translation="MDLPQLRTPCVMVIFGIDGDLAKRKLIPAVYNLMAGHFLPEGFA
                     IVGLDRPEMTTAEFREKLDRMMGDYLPATVDRSVWQALSERVHYLAGDFQEGATYRRL
                     NELLQQVDASSGTQGNYIFYMATIPGLFGQIVTHLGEYGLTVEREGTWRRVVIEKPFG
                     HDLESARALNHELQRVLQERQIYRIDHYLGKETVQNILVFRFANGIFEPVWNRQYIDH
                     VQITVAESLGVEHRGRYYEQAGALRDMVPNHLLQLLCFLAMEPPNSFAADAVRDEKAK
                     VLRGVVPLSSLDVLRFVSFGQYGPGTSDGKAVTGYRSEPHVGSESMTETYVAMKLFID
                     NWRWAGVPFYLRTGKRMTRRLTEIVVQFKRAPFMLFRKTQVDRLVPNVLVIRIQPDEG
                     ISLRFDAKVPGPTVRIGTVDMDFQYADYFGNAPQTGYETLLHDAMAGDATLFQRADNI
                     ELGWAVVQTILDVWKSLPGPASFPNYPSGSWGPPEAEALLKREGREWWNGGQA"
     gene            38544..39371
                     /locus_tag="PKK81_01405"
     CDS             38544..39371
                     /locus_tag="PKK81_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNV31551.1"
                     /translation="MMAGAGFVSGTTPQLTPRDVHIEVPADSLFGSLPVNRGLTVSIH
                     VPEPPVSRGEPLVAVFETPSSAPYTVPLEIDRSRHHYSATVDLGRLSGTMGTPPKATA
                     LQVLIGRRQGLHVEALVRRTVVVTLAIPGYADHRSARTDFANQMANGSGPPRNQPADL
                     ALLDGQVEEEELVGNGGLPQQEGYWKMLQGLIHKGMPDGAATRRGREIGRMPGIGFRL
                     YANGEAQLIEVERSSGDLELDQAAVLAVVNAHPFPPFPPGTSDTHVDVHVEIPGLPR"
     gene            complement(39405..39725)
                     /locus_tag="PKK81_01410"
     CDS             complement(39405..39725)
                     /locus_tag="PKK81_01410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV31552.1"
                     /translation="MERRDSPRYEIEVPLTFSGNAIAGSGLVTSLSNEGCHVVSEESL
                     PARACLSLHVEFPAPYEPMTVEVAEVRWTNATGFGLVFVHVHDEEQTRLQRFIDWLKR
                     THNN"
     gene            40104..40226
                     /locus_tag="PKK81_01415"
     CDS             40104..40226
                     /locus_tag="PKK81_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31553.1"
                     /translation="MEHLSEIVITVVAFAALIGQWMTDLRTMPNRNRPTSPEEQ"
BASE COUNT         8772 a        12755 c        10690 g         7889 t
ORIGIN      
        1 cgttcctgga gacgctgatt gtcgaggcgc agcagatcga gacgatccaa caattgctcc
       61 cgttggatat tcgtccattt cagcgaccgc ctcaatccag ccagctctaa ctgagcctgc
      121 tgtccatcct caaacccgga gcccatgcgt gcgcctcagt cgatcgccat ccggcagtag
      181 atggcaaatc gtaacgcact ctcggtccat tgcggagtga ttactgattt atcgacagag
      241 acggtgagcg gagggggatc aaccgaatgg gatgaataca cccggagcgt gaggatcaac
      301 gcgcgcgggc acggatcggc aatgcgccgg tcgaaccagt ctctcctgcc tcccagcgat
      361 agcgaacaag aacgccgtta gcctcccatc ccggcgggca cagcttgcct gtgccatagt
      421 agtcggcatc cagcacggaa aagggcgcca cattcttgat ccagtcctgg gcatggccgt
      481 tcgactcgat ataggattgg aagaaatacg cgccaccggt gagcggcaac tcaggcaaaa
      541 tgaaatcgac atacccttca ccacgcaagg tcaagggttt cggatcgacg atgtcggtgg
      601 ataaaacgaa caggtcttgc gccttccgtc cattcactga aatgctgacg cggcaattca
      661 aaaattcttt tcccgccgca caacggtagt acatacgaat caccacatct cgcccggtga
      721 tcgccgctgc gacaggatgc ctctgagtat ccatcacttc gatcctggtg agcaggatct
      781 cgccgagtcc ttcccggtca tgacgcgctt cgatcgccgt ccccgccaga ccccgaatgg
      841 catcggcata gccctcaacg acctcgtgcg tgttgccgat cttagcgaca cgccccttct
      901 cgagaagaat cgcgcgctca cacaaccctt cgatcacggt catgttgtgg ctgacgatca
      961 gcaccgtccg cccgcttcgg ctcacttctt ccatttttcc caggcacttc tgctggaaac
     1021 tggcgtcgcc gacggccagc acctcgtcca cgatcagaat ctcaggctcg aggtgtgcgg
     1081 cgactgcgaa cgccagccgc acgtacatcc cgctcgaata ccgcttgacc ggtgtatcga
     1141 tgaacgcttc cacacccgag aacgccacaa tttcatcgaa ctttcgccgc acttcccctc
     1201 gtcgcatgcc tagcatcgcg gcgttcaggt aaatgttctc gcgaccgctc aactcggaat
     1261 gaaaccccgt acccacttca agcaacgagc tgacacgccc gtaaatatcc gccgttcccg
     1321 tcgtcggctg agtgatcctg gaaaggatct tgagcaaggt gctcttcccc gcgccgttgt
     1381 gcccgacgat tcccagcact tcacctcgct taacctcaaa cgagacatcc ttcagcgccc
     1441 agaacgaagg gttcgactcc ggtggaccac caccccatcg cgtcaggcct cggaggcgat
     1501 gcatcagatg atcccgaagg gtattatgct gatggcttgc ggctccgagc cggtagtgct
     1561 tcgacaggcc gtctgctttg atggcaatat cgctcattca aatcacgtcc gcaaacgttc
     1621 gttccatcga acggaaatag atgagtccgc cgacaaagac gacgaacacc acacccagac
     1681 ttggtaagaa catcataaaa tcgacctgaa acccgggaat gagtgcccag cgaaatccct
     1741 cgaccacact cacgaccgga ttcagcgcat agagcgctcg ccaggactcc ggcaccttgc
     1801 tcacggggta gaggatgggc gacacgaaca tccacagctg gatgaacatc ggtaggatat
     1861 gcccgacatc ccggtacttc acgtacagcg cggagatcca gagcccggcg gccagcgtaa
     1921 tcgtggcggc cagcgcggcg aacagcggca gggacagaat ccgccaggtc gggatgattc
     1981 caaaccaagc catgaggccg agaaacgcga caaatgccaa ggccagatcg accagaggcg
     2041 cgatggtggc cgaaaggggc agaatcaggc gtgggaaata cactttgctg atgagcgggg
     2101 cttctcccac taaactggtt cctcccctgc tcaacgcctg agacacatag aaccacggca
     2161 gcaggcctgc gtagaggaac accgggtagg gaaatccgtc ggatggaatt ttccccatga
     2221 cactgaatac aaccgtaaaa atgagcatgg tcgcgaccgg ctgaaacatc gaccaggcga
     2281 ctcccagcag ggtttgcttg taccgcacgg tcacatcacg ccagacaaga atataaatca
     2341 gctcgcgata ctgccagagc gcggccaaat ccaaatccaa taggccgcgg gcaggctgga
     2401 tacgcacgtg cggtaaggtc gacgatggcc tctggtcgac agtcaacgcg gacatggcaa
     2461 tttcgtcact cctcatgatc ctcacaccga cggcaaccgt atcgaaagac cggcccccgc
     2521 gcctcaaaaa caagagggcc tcacagccac attacacttg gttctaactc gaaccgactg
     2581 taatcgttta tgtcaaaatt ttccgctttg gtcaagaaat tcgaactgtt atgccccttt
     2641 ggggccccag cactgcatgg atcgccgaat cccggcccca cgtcgagcag gcggaaattg
     2701 caggacgccc actaggaaaa ctccatcacc tcaattgcca ggcaatacct tgaagaatca
     2761 actataccaa atgactctgg atgaggaagg atacagggtt gggagcagtc acgtgtaata
     2821 gaaacgatcg gctacggtgc aggagggaca ggttttggag cggcacgcaa ttcgtcggca
     2881 gagacatacc gaatcgctaa cccggacatg tggagcatcc agccctgccc accaccggct
     2941 ccctgcgtaa aactccacgc atccattcga aacggattcc aatagccgct gcgataggaa
     3001 ttgcccgacg tcgctcccgg taccgccgtg cccatggtcg attggaatgt cgggcctcga
     3061 tctgcgaggc gccgcacgtc cgcatctcca aggacaacag catcggcccc caactctttc
     3121 gcccgggcga gaatccgatc acgcaacgtg tcttcgctgg catactcact ggtcgcaatg
     3181 atgcgggcga tctgcacgtg tccatgattg ggctcgcgct gtaacgtcgt cacgtcctcg
     3241 cttccaggtc gaggctcgaa cgccttgctg gtcaagggat ccacctgcac ggagacacag
     3301 gcgctcgaca acccaagaaa aaggatcaac gcaaggcggg aagcctgctt catcatgact
     3361 ctatttcgaa tattcatgtc caacgatcca agctccgacc cactttggct tgatcgagtt
     3421 ccatagtgac ggcttgggag gtccaattca acccccaatc actccgtgtc ccgatggtac
     3481 ccgtcaccaa ctgcggcgtc aatccggaaa ctcttgtacc ccactcaatg ttgtcatgtt
     3541 ttacactaca gcctgccttc agccagcgct cgaatctcct ccatccgccg tcgattgacg
     3601 ccaagatcac tatagccggt tcgggaggcc gaccgaaaat ggatcgtctt cgtcgcatca
     3661 tcaaacacga attcgacatc gtccacgaat cgaagcagca ggctcgtaaa ttcgtaatgg
     3721 aggtaggcct cgtcctcttc aacgagtttc gcacggggta gattggccac aatggttttg
     3781 agcgtctctt tggcccccgc cagagttttc tggtatcgat acggcacgat tccatgccca
     3841 tgatcagagg cctgggtcga cacacaattc ggactggatg ggcagggctg gagtgatctg
     3901 atcattgcgt tatcaacaat atatagtgcc tacgggcacg ggctccaagc cggagacagc
     3961 tgaccagata cactaccccc aatatcccaa cgaggcttaa cacctgatta ttgaactgta
     4021 atgagaggcc cctcccctgt caaccgctgt accagaggtc tggcaagctc ctgcctatgg
     4081 gctttagttg aggtgagcaa ccaaccaggc gtgacaccgg ggtacggcag ccatcgccgg
     4141 ttgttcaatc aggcgaaacg tggcaaaaca cagcagaatg agaacgggca cgaggcctag
     4201 cacaatggac caatgttcag cggccgagag agttgacgcc cattcgccaa gtaccaaccg
     4261 ataggcggca aacaacaaca ggctatgcag taggtacaga ctgtacccca tttcgctcaa
     4321 caggacagac gtcggccatt caagaatgcc gtagattgag ttcccacagg cgataatcgt
     4381 gaacgcgccg gtcagtagca tcaacgcggg aaatgtataa gccgtcggaa agagccacat
     4441 agttatccca agacaagcga ccacgaccaa cccccagact cgatgggcga gcctcgtacg
     4501 tattgcatca acacgcgcca ggctcgcagc cgcgatccct ccaccgaacg catacaacac
     4561 agggccgcga acgtccggca tcaccgtcgt gagtgtcacg actgccgcgg cgctgaacag
     4621 cagccacgag gtcggcgtcg ccgcacgaag gcaccatgag ccgaggggca ggaatgcata
     4681 gaacaaccat tcatacggca gagaccacac ggccccggcc aagggaatcg tttcggcaaa
     4741 gccattgacg ggcgcaatcc caggaatcgt aaacgtgagc caactcgcta ggtgcagcgc
     4801 aaaggaggtc atcgattcct gcagtcgaaa tccggcacga tagagcgcga tactcaccac
     4861 accgatcacc atcaatagat acaacggcac cagtcgaaat atccgggaaa ggtacagtcg
     4921 cgaccagtcg accggttgca ctcgcccgtc gagtagcttg gaccaaaaga gaaagccggt
     4981 aatcatgaaa aagagggtca cgctgctttg tcccaagtgc gtgtaaaaat tagacggagg
     5041 gacatcccac gtgccggaac gcagatagaa ataccagacg gaagaatggc tgagaaacac
     5101 ggcgaaggcc agatatcccc ggagtccatc tatacacgaa tatcgggctg ccgatgtagt
     5161 tcgaagatgg ccgatgtccg gaagaagtgt ggcagtgccc actgcaagca agagcgccgc
     5221 gacagcaggc cacaggctgg tagcatccat gttattggat gacaccatag gagttcacat
     5281 ttgtctaacc tttcccgcaa cctcaccggc gtatggcttc cgactatacg agtccggctc
     5341 ccagcccctc gcccaaccac caccgggcat caagcctctt cccatagcac tccgttgatt
     5401 gccggagtga tcctgcggta ccctagctcc ttattgatcc caggttgagg gcgaacagcc
     5461 gctcaccgat tgcacacagc tgagcgctga cgctcaggtc aacaaaggag tcctcacgca
     5521 tggccggttt cccgatcgaa gtcgcaaccc gcattaagac gctgccgcct tacttgttcg
     5581 ccgccatcga caagatgaag caggaggcac tcgcgcgggg agtcgatatc atcaacctcg
     5641 gtatcggtga ccctgatctc cccacgccca cgccgatcat cgacagcctg gcccaagccg
     5701 ccaaaaaccc gaagcatcac caatatccct cttatgaagg catgctgtcg tttcgtaagg
     5761 ccgtcgcgga ttggtacaag cggcgcttca atgtggcgct cgatcccgcg gatgaagtgc
     5821 tgaccttgat cggatcgaag gaagggatcg gccacgtcca tctggcattt atcgatccgg
     5881 gagacatcgt cctcgtgccc agcccagggt atccggttta tccggtcgga acaagctttt
     5941 gcggtggcgt ctcccacatc atgccgctga caaaggccaa cggcttcctg cccgacctca
     6001 acgccatccc gaaagaagta gcgaagaaag cgaagctgat gtggctgaat tcaccgaaca
     6061 atcccacctc ggtgatcatg agcaaggact acttcaagcg cgtggtggaa tttgcccagg
     6121 ataaccaggt gatcgtctgt cacgatgcgg cctactcgga gatttactac gacggcaagc
     6181 gtccgccgag ttttctcgaa gtggacgggg ccaaggatgt cggtgtggaa ttccactcgc
     6241 tttccaagac ctacaacatg accggctggc ggatcggatt cgccgtcggg aacaaagggg
     6301 tgttggccgg actgggccgc gtgaaaagcc agttggactc aggcgtattc gaagctgtgc
     6361 aagcagcggg aatcaccgcg ctcgggttgg acgattcggt caccgatgga ctgcgaaaga
     6421 tttatcagga acggcgcgat accctcgtgc ccgggttgaa gaagctcggc ctggaagtcg
     6481 atccgcctcc agcggccttc tacatctggg tgacggttcc caaaggttat acctccgcct
     6541 ctttcacagc ccatctcctg gagaaggccg gtatcgtcac gacacctggg aacgggttcg
     6601 gtgccccggg cgaaggctac attcgcatga cggtgtgcac gacgaaggaa cgactggcgg
     6661 aagcagtcga acgaatcaag aaagccggat tctaaggcaa gcgtgacggc ccctcttcca
     6721 ttctgagaag aggggcccga tacgaatatc ctcatgtgcc attgagccct gtcaggacaa
     6781 cgatggccac agcattcatc ggtttcggct ctaacctggg aaaccggatc gacttttgtg
     6841 atcgggccgt gacactgctc ggcctgctcc cacattcacg gctggaggcg gtgtcatcgc
     6901 tctacgaaac cgaaccgctc gaggatgggg ccgcccccgg cccctcctgg ttcctcaacg
     6961 gtgtggtaca gatcgaaacg aacatcgccc cgaagagtct gctggaaatc tgccgtgaga
     7021 tcgagcgggc gctagggcgc gatcaagaca accgcaaggg gcctcgcacc ctggatctcg
     7081 acgtactgtt gtatgatgac tgcattctcg ccgagccggc cctgaccgtc ccgcatcccc
     7141 gcctgcatca acgacgattt gtgctcaccc cgttggtcga actcgatccc gagcgacgac
     7201 atccggtgct cggacaaaac ctgcgcgatc tcttatcgca actcactgat ccatcgatcg
     7261 ttcgcctgct cgagccccaa ccgaactccc gctatggatc acgtccaacc tgcagcgcac
     7321 ccccaacagc agggccacaa gcgtgaagat cattcgcgcg acgccggcca tggccgactg
     7381 gagccgccga ctccatcgag aaggcatcac gatcgggttc gtgccgacga tgggagcctt
     7441 acacgaaggg caccgcgcct tgatccgtgc cgcccgactg gcctgcgacg ctgttgtggt
     7501 gagcatcttt gtgaacccga cacaattcgg gccgacggaa gacctttcga aatatccacg
     7561 ccagcttcga cgtgatcaag cactgtgccg aaaggaaggg gtggatgtca tttttgcccc
     7621 caccgtcagg acgatgtatc cgaccggcgc tcaaacggtt gtgactgtcc cggggatagc
     7681 ccggcgctgg gaaggggaag cgcgaccgca ccattttcaa ggcgtggcga cagtcgtcac
     7741 taagttgctg tccctggtcc ggcccgacaa gacgtggttc ggacaaaaag actatcagca
     7801 agccgccctg gtgcaacaac tcgtgaagga tctcaacttg cccgggcttc ccattgtcca
     7861 tccgaccgtg cgggaggccg atgggctggc gctcagttca cgcaatgtct atctgtcgag
     7921 ttcagagcgg caggcggcgc ctgtgctctc tcgcgcactc caggccggcg tagcggcgat
     7981 tcgtgccgga gaacggaatg gaacgcgtat ccggcgccac atgctgcgcg tggtcgcgac
     8041 agagccgctc gcaactgtgg agtaccttgc agtttgtgat cctgcgactc tcgagccgct
     8101 ttctctgctc aaccgccagg ctgtcctact cggggccatc cgcatcggga cggtacgcct
     8161 ccttgacaat gtgctgacgt cattccgccg caagccttaa catcgaagct tcacgcgcaa
     8221 tcgcgacccg acgccggaag gaggcgggag tcacgcgaat gccacgaagt tgaaccgtgt
     8281 ggttgttatg cgctgacgcg gggaggcgct tgttgatgtg cgaggagctc gaggaccagc
     8341 tgacccatac gctgcttgtc atcaggttca aactccagga acttcacccc gatcgacagc
     8401 ggctggatgg agcaaatcat agcggcgtcg actttgatat cgggctgtcc ggcaggagca
     8461 tgcacgatca gtttcacgaa cgtaccccgc gggagcgagg aggatgcttg caacgtacag
     8521 ccgcccattg agatatcggt gatcaccccc ccgactgccg gctggtccag gatcagcgac
     8581 gcggcgaagt tcgcccgcag ccggcggtac tcacgccgat caaactcttg ggaggtctgc
     8641 cggttacccg gccggcgggc ctgaaagcga gccgtgcaga gctgacaacg aacttgaaag
     8701 atggacaagc ggttcagtgt cttctccatc aaattcgtcc cggatgtcac tctgacacag
     8761 ggcgtgccgc acgccggaca atacagctct ttcatgctac gactctgact cctgcattct
     8821 tgtgtccact tgcgatcggc ccgccaaact cccggaggat tgcgccgcaa gcagcggttc
     8881 cacctgggcc ggagaatagg tcggcggttt tacccacttg ccatcggctc gcttgtagcc
     8941 acccactttg ctcaaattcg accgatgcac ctcacgaaac accggatcca tatccaatcc
     9001 ataggacacg gctgtgccat agaccacata caacagatcg gccagctcct tcgccacccc
     9061 ggggagatcc tgggcgacca tggcctcctg cagctcttcg aactcttctt gaatcagccg
     9121 cacccgaagc tgtcgggtcg cttcttccgg aagagaaggc cggtcgctga cggctatatc
     9181 gaacttgcgg tgaaactctt ccaccatcgc ctgcggatcc atcatcctaa cgtccctcta
     9241 ctctctgccg caaacgcaac tccggtcctt ggttcgatgc cggaggcttc cagtagcgca
     9301 atgtctttat cagatgatat cccgaggtct ttgaattttc tagctgccgg cagcacgcga
     9361 gactccaaag atccgaccgc cttattgtag gcccccacgc ttttcccgag cgcctggccg
     9421 atctcgttca ggtgatcggt gaggacggcc atccgttcgt acaattcctt ccccagtcgt
     9481 cccgcctgct cggcatgttc gttcaactgt tcctgtttcc agccataggc caccgcgcgc
     9541 aagagcgcca tcagcgtcgt cggcgtcgcg agcacgaccc cttgcgcaaa cccgtcttcg
     9601 atcagcgtcg gatcctgctc caacgccgca ccgagaaact gctcccccgg cagaaaaagg
     9661 acgacgaatt caggcgtctg cccgaactgc gtccagtagg ccttgagact gagcgcgtcc
     9721 atcctggccc gcacctgacc ggcatggcgg agcagatgcg cttgacgctg ccggtcatcc
     9781 tccgcctcat aggcatcgag gtaggcggct aagaccgttt tggcatccac cacgatctgc
     9841 ctgtcccccg gcaggtacac gaccatgtcc ggacgaatga gcccttccaa ggacccgatc
     9901 gtatcctgct ccacaaagtc gcaatgggcc accatccccg caagttcagc gacgcgacgc
     9961 agcgtcatct caccccatcg cccacgcacg gacggagccc gcaaggcctt caccagattg
    10021 ccggtttcct gttggagccg ctggtgcgat tcagccatga atctcaactg ctggtcgagt
    10081 cctccatatt cccgctgccg aacctgttca gcctgctgca gctgttcttg atatcgctgg
    10141 agcgattcct ccaagggctt gaccagttcg ccgatggctt gctgccgctg cgacaactcg
    10201 cctttggcct ctgcctgcaa ggtggcaaac gacgtgcgcg cgaggtctaa aaacgcctca
    10261 ttgttctgct tcagtgcctg gccagacagg gcctcgaatg agtcgtggag ttcttgccgt
    10321 gcctgcgcca gcagcgcctt ctggtcctcc aaactgcggg cgacctcttc catgcgggtt
    10381 tcggctttgg tccggccctg ctgcgactcg gcgagcgcgt cgcgcaactg ccccatctcc
    10441 gactgctgat catccactcg ctgctgcagc gaagccgcca cagcatcggc gcgctgcgcg
    10501 cgctctccgc tctcaagcaa ccgggcatgg tggcgtgatt ggacacgaac gatcacccac
    10561 catccggcca ccagaacgcc cacggcgaga ccaacggcgg tgccgaccaa aaacgttggg
    10621 gaagtgaggt cgatcatggc gcttccctac gaatgcaccg actgactgga aatccattca
    10681 cctaactgtt gtagggaaat cgcgtagagg gtacgagaat ccaggagaaa cgtcaagaga
    10741 ccaatcgact gatgcggcct gtggaacttg cgatttcgac ctgttcattt tcgcgcaata
    10801 gactggtggc atagggcaca ttgaccagga ccggaaggcc gtattcacga gcgatgatcg
    10861 ccccgtgcga gagcgtccct cccatttcgg cgatgaggcc ggccgccatt ccgaataaag
    10921 gagccatacc gggatcgatc accggaacaa cgagaatgtc tcccgctcgc acccgtgccc
    10981 aatccgccgt cgaccgaaca agccggaccg gtcctttggc aaccccagca ctgatggcaa
    11041 taccccgtaa cacgcctccc tgcccctgat cgcttgactg cgccccctgc tcgacgaccg
    11101 cctcccaatc ccgaatcgtg tcgggcacct gcagggcctc atactgaagc cgttcctcgc
    11161 ggcgccgacg cacgaggctc tgccaatcac gacttgcgcc atcagtcagt gcgatgcgtt
    11221 catccagcgt gagataaaac acatcttccc gcacggcgaa gcttccgcgt gccaccatga
    11281 gctcaccgag ccgaagcaac agatggcggg ctgctgtgga gtaatacatc aggtgatggc
    11341 gattctcctc tcgcaaggca cagaagcgac tgagcctccg ataccaccaa cggaacacga
    11401 gccaacgatg gcgtcgccac ccgaatcgcc gggcgatctc actgagcgcc tgttcgcgtc
    11461 gctgagcctg gcgggaaaga acttcttggg gtggcgccgt cacgccggcg cgcacctgtg
    11521 cccgcaacag ggccaacacg gcatcgggct gatctgcaat tcgtggggac atgatatcgg
    11581 actctccgac tgcacgatgc ccgtactcgg ctaagtactg atcaaacagc cgcaggaact
    11641 ccgtcccctg aatcgcgtcc cgataaccac acgccgccca ctcctccgag aaaaaccatt
    11701 gtcgaaccgt ttcttcttgc tgtgcagcta cgacaagctc agccagccgg acaatttggg
    11761 aggcactgat gacggtcccc tgtccctgca atgccccatt gagcaactct cgccagtctg
    11821 aaccaagcca gcccggaagc accgtgccca tagcctgaag actctgcgcc actccacccg
    11881 cgattgcgaa ggtcacctca tgatcgtcga gccaccgccc tatgctgtcg agaattgcgg
    11941 ccaattcctg caccgacaga ttttggatgc gatcatagcg gtagcgttgc gccatggcct
    12001 tcatggcaga aaaattcttc ggcccctgct tcgcggcctt acgccactcc cgcagcatac
    12061 cgactccggc acgaagaagc gcgagcgccc cgagaggccg taccgacggg gtaaacatca
    12121 ggggctcacc acccatctgt tcggttaaga acgccggatt cccatgaagc tgcatgacca
    12181 aggtgtaaaa cagagtcacg ttgagatagg gacgtccatg gaacacacga accgacgaga
    12241 gcccttcggg gaccgtgcaa ccgagtcggc gatagggggt gatgatgtag gcatccatga
    12301 atcgctcgag aaacgacagc cctaacgggc tgggcaattc cggcaaggtt tctttgaaat
    12361 tagcccgcgt ccactcacat tcgtcattgg tcagagaggg ccacggtcga acggcggtaa
    12421 tcggacgggc ctgcacgatc cagagtcgct ccacatccaa cacccattcg agatcgaccg
    12481 gttgacgaaa cgcgacttca atgcgcttcg acaaccgtgc gagctcgaac agctgcttgt
    12541 ctgtcaggca ggcctgccgt tgttccggtt cgggaatcga ctcttcccgc acacctccag
    12601 gggtggcggt cagcttattc cgtttccggg caagcatgcg tcgtcgtacg cggaacggcc
    12661 gatgatcgtc atcgtatacc tccaccacat actgatccgg cgtcaccact ccattgacca
    12721 gggaagaagc caatcctggc acggcatcaa tcgacacctg agaggttcga cccgtgacgg
    12781 ggtgaataga gtatgcgacc cctgctgcct gtgcgctcag catgggttga atgacgactg
    12841 ccatggcggg acaaggccgg tcggcaccgg aacgaagaaa ataccgaaac acccgctcgt
    12901 cccataacga aatccaacag tcacggatag attgcagcac ggccgccagg ggcacgccca
    12961 gggtcgtgcg atacagaccg gcagcgctca tatccgcagc atcttcattc gtggcggagg
    13021 accggacggc ccagcgtgtc gaggaatccc cggccagccc tgtcagtcgt gtttccaggt
    13081 cggccgggaa tcctgtcggc cagggctgct ccagcaagag ccgctgaatt cgtgccagct
    13141 cctgtgcccg ttgtccttca gacaagtggg gtagcttcgt ccagagtgcc accgcatcga
    13201 caccagcctg atccaggccg tgttgataga ctgcagtcgt cacacacagc cccttcggca
    13261 caggaaggcc agcagtgagt accttcgcca ggcctgcagc cttgccgccg acaagaaagg
    13321 gagcacagct ctcatacaga gagaggagga acggatcagc catagagggg gatactagct
    13381 agagtgcgag gaggaggtaa agatcgttca cattggtccc ggtcgggcca gtcacaatat
    13441 gactacggag tttcttgaag aacggatagg catcgttgcc ctgaagcgca ccaggcggat
    13501 caagcttggc gcgccgggcc cgcgcagtcg tttccccgtc taccaccgcg cctgcgacgg
    13561 aagtcggtcc gtctgtgccg tcagtcgcaa aacctgccac ccacacatca gacaacccgg
    13621 caatctccaa tgccgccgcc aaggcgaact cctgggctcg accgcccttt ccctgccctc
    13681 gaatcgtcac cgtaggctct cccccggcga tgacacaaca gggacgagcc aaagggcgac
    13741 catgcgtcgc aatctcccga gctatcgcac cgaaaaactt ggcgagttcg cgagcttccc
    13801 ccgtaacggt agtggaaagg acaagcgtct gtagccgctt ccccttcgcg gccttcacga
    13861 cggcatcgac agccgcttga ttgtctccaa tgagcagatt gtctacacgg cgaaagagta
    13921 cggcgccggc tttgggagtt tcgggcaggg tcttcttcat gcccgcctcc aggcgtcgac
    13981 gcaccgaagc tggaactttc cgggcaacct cgtatcgctc caaaattctc caagcgtccc
    14041 gaaatgttgt ggggtcagga gcggtcggac cagacccgat cgtaccaaga tcgttcccga
    14101 tcacatccga gaggatcaca ctcgccacac gcgcggaggt cgctgcggca agctgcccgc
    14161 cttttacaca agacaagtgc ttgcgaaccg cattcatttc gtgaatggtc gctccagacc
    14221 gcaacagcag cttagtcgtc agctgcttgg ccttgagggt gaccccagga gcgggggagg
    14281 gaaggagact cgaggcaccg ccggacaaga ggacaatcag caggtcgtcc gatttcagcg
    14341 tcccgattaa tgccatcatc gcacggctgg cctggagccc cgcctcgtcc ggaaccggat
    14401 gcccggcttc caggatgcga attttcttcg tcggagcacc atgcccgtat ttcaccacga
    14461 ccagtccggt gtcgatcctc gcccccagct gccgctccaa cgcctgagcc atgcgacccg
    14521 acgccttccc ggccccgacc actacaagtc gctgatgttg tgaaagagga tagcgctccg
    14581 aaccgatagt gagttgcccg cgccggaagc ggacctgacg gcacaccgcc tcgtaggggt
    14641 cgacggcctg cagtcccgca cgaaacagtg ttgtgaagag ggctcgtgcc ctgagctgcg
    14701 agactccgac ttgtatcatg ggcactacgg gagcttctct tgcttgaaac agcgattcgg
    14761 acaggtttgt cgcggcacct taagctggta ggtacccttt ggcaagtagt ccttcgtgaa
    14821 ttcgggattc agcatcttca attcaagaaa ataggttccg tattcctcag cgatcgccgt
    14881 gaggcggcgt tgcggctctt tgatggtaac cgtcaccgtt tctgtttcaa ggggagggta
    14941 gagatccttc ttggtcagac caagatactt ctccggctgg gaatagatct ctttcgccgc
    15001 aataatgcgt gccacatacc gcatggtctc tcgtgggccg tgcatgcgcc agtagtcatt
    15061 gatcttctgt tctttgatca atttccgcac gcggtcttcc ccggcgttgt aggacgccat
    15121 cgccaggaac caatctccct gatacagatc ccgcagatac cgtagatact tgatggcggc
    15181 ttcggtggac atttcaagat tgcggcgttc atccagccag cgttcgttat ggagcttata
    15241 ccgtcgccct gtggaattaa tgaactgcca gggacctgat gccttggccc gggaataggc
    15301 ggccgctata cacttgctct cgaccaataa catatatttc aagtcatccg gcaatccggc
    15361 ctcagccaac tgcttttctg ccggcgcaaa acatcgacct gtccgtttcg ccagaatgat
    15421 actttcacct tgatcctcga ggaactgata gaactcgtac tcgatccgct cgcgtacctg
    15481 ccagttatcc aacggaatcg cctgcccggc gaacgtcagc ttatcgggca acttgaagga
    15541 gctgaggaag aagcgctctc cctcccgttt gatttcgggg aggatgacca gtcgatcttc
    15601 aagctcaggc accaactccg actcgcccgg cggcgcgacc ggcacctgat cagactcctg
    15661 ggcgttccgg ccctcctccg ccttagcgcc ggtcaacgcg gcagaactcg aatccgccga
    15721 taaaaccggc atggcggaag acaggccgaa cagcattgtg acgcacgcta gaaatgcctg
    15781 aacaccccgc tctttgttcc acatgcccct gtcatccttt cgtcgcgact gatggctcat
    15841 gctagcaaca agctcgccgt gggacaagca tctcctggtt ttggtacact tcgcgcctat
    15901 gtccgccctg acaatgctca acaacgagat cgtcgcctgc acgtgctgtc cccgactggt
    15961 tgcctaccga gaagctgttg ccagggataa acgacgccaa ttccgcgagt ggtcctattg
    16021 gggtaagccg gtccctggat tcggagattc caacgccagg ctctatatct tgggcctcgc
    16081 gcccgcggca catgggggga accgaaccgg acgcgtattt accggagacc gtagtgggga
    16141 ttggctgtat gaagcgttgc accggttcgg gttcgccaac caggcgacat ccgtacatgc
    16201 gggtgacggc ctcactctca ccgactgcta tatcggcgcg accgttcggt gcgcgcctcc
    16261 ggcaaacaag ccgacgccgg atgaattcac ggcctgccgg ccatttgtcc tgcgcgaact
    16321 acggctattg aagcgaatgc gtgtgatcgt caccttgggc aagattgcat tcgatcacta
    16381 tctcaaggcg agccgtgaat tggggctagg aactcccggg ctacgccctc aatttggaca
    16441 cggagtcgtc tacgacttac cttggggcgt gaccttgatc ggctcatacc atcccagcca
    16501 gcaaaacacg ttcaccggca agttgactcg accaatgttc catcgcattt ttctcaaggc
    16561 ccagcagaaa ctcgccgagg caccattgcg cgattagccg cttcgagagt ctatttcttt
    16621 cccattgcat cccaatcggc cagaaacttc ttcagcccac tgtcagtcag cggatgcttc
    16681 acaagctgct gaaacacggc gtaaggcatc gtacaaatgt gccccccggc caacgctgct
    16741 tcgacgacgt gctgcggatg ccggacgctc gcgacgagga cctgcgtttt ataatcgtag
    16801 ttcttataaa tcgtgacgat ctggcgaatc agtgccattc catccgaact cacgtcgtcg
    16861 agacgaccga tgaatggcga gacacaccac gcgcccgcct tcgcggccag caaggcctgc
    16921 gtcggggaga agcacaaggt gacattcaca cgaatcccct ctgccgcaag cttctttgtg
    16981 gcccgaagac cttccgggat aaggggaacc ttcaccacga tattcttgtg aaccttggcc
    17041 agatctcgcc cttccttgat catggcctcg gattcgacac caaccacctc tgcgctgatc
    17101 gggccatcga ccatcttaca gatttcgaga agcatctcct tgaaactgcg cccttccttg
    17161 gcaacaagag atggattagt tgtcacaccg tcgatgaggc ccaggttggc accctcttgg
    17221 atttctttca cattggccgt atcaagatag atttgcattt gaagatcctt tcctcgctct
    17281 gagcattcga acccactttt cctcaagtta ttgtagaagc aactcgatac caaggcaata
    17341 agtcagggct gccgtgctag acttgtttga cagatgagaa tggtgaacgc taagattttc
    17401 tccgcaatag acaataatgt cgcgcttcac acccctgatt caggaggaca acctgtatgc
    17461 gcaaatccgc gtggcttata gcgatcgccc tcctgtccgg agcctgcagt cacaatccat
    17521 acctagatgc ctctctcact cgctatcaag gaaaagatcg tgcgtggatt gaaaaggaat
    17581 tgggcgctcc tgatgccaag acgtcccgat ttttcggtgg agagaagtgg acctacaatc
    17641 gaattgcggg ggggaaggca ggaccgccgc tatttaattt taaagcaaac gaatgtcagt
    17701 tgatcctgtt ttttaataag gacgaaatgg tctctgactc tagctattcc ggctgctagc
    17761 tagaaagttg cttctgaaat gtatcggcgc aactacgact gcagaaataa taggtctgcc
    17821 ctccaatctc ctctctcacc gcattgccac gagggatgaa gacacggcac accgggtctt
    17881 gaatcatttg atttccagcc gcctgctctt cttgaaggct caccgccccg ccatcctctt
    17941 tcatttttct aattgccctg cgaagcaggt agtagaggag cacgagcaaa ccggaaacca
    18001 gcaataatct atacatggta gtgcatcctg ccagcgccac taaatattag gccatcgtcc
    18061 atgacgtgaa aacagcgacg aaccctaccc cgccatctta ctagcatgga acaatcccgt
    18121 aaggggcaat cttaggacca aatggcccag atgcttttgt gtctatgagg ggccttttcc
    18181 catccttata cagtacttca gatggatggt ttatcctaca gcgcaatgtt ataagccagg
    18241 aatgcataaa tgtgacaaat gccgcggaac aatgttgccg gaacgggcgg ttgacctgaa
    18301 tgctggcttg gcgatcacag tccttgcgtg cctcaactgt ggccgacgaa aagcagctga
    18361 cgcagaaccg agaccgatca cgtcacgaca ttaacccgtc gcaataataa tccttcacag
    18421 gacgctcaca tgcgttgagc cgaagttggt ttaccccaac ttcctctggc cttttgtcca
    18481 ctctggactt gatgcccctc tcatcgcaga gcaatcctcc cctttatcct actagccaat
    18541 gcgttgagct aactgccagg tccgcccagc ttttctatcc aatccccaag attttacgtt
    18601 tctacatatt cacgcagtga tcccagcaac aaacgaattc tcatatcagg agcacaaaat
    18661 cattccaggc gtcttggaat gaaaaagagg tagcctcgga gggatttttt acttgaggct
    18721 caatacatcc agcatgtcat agagaccggg tggctgcttc acaacccagc gggcggcacg
    18781 gagggctcct ctggcaaatg tgtcccggct actcgcacga tgagtgagct caattcgttc
    18841 gcccatcgtt ccaaacatca cggtatggtc tccgacgatg tctccggctc gaatggtctg
    18901 aattccgatt tcgcccctct tccgctcacc gataagtcct tttcgcgcat aaacgccgac
    18961 ttgaccaagg tctcgattga ctgcgcgagc caagacctcc gccattttga gtgcagtacc
    19021 actcggtgca tccttcttca ggcggtgatg ggcttcgatc acttcaatat cgtagtcctc
    19081 cccgagggtc ttggccattt cggcgatcac cttgtaaatc acattaaccc ccacactcat
    19141 attgggggaa aacacgcaag ggatctgctt actcacactt cggagctcat ctaactctga
    19201 cgatgaaaac ccggttgtcc caaccacaat gccacgtcgg tgctgcgcca caatcttcaa
    19261 atgcccaaga gttgccgcag gcgttgtaaa atccaccacg acctccccac gctccatcaa
    19321 actggaaaga tcgtcgacga tcggcacgcc tgttcgccca caaccagcca cttcaccgga
    19381 gtcttccccc acagcatgat gccccttgcc ctccacagct cctgcaaggg tcaagaatgc
    19441 agaatctttt accaaggaca cgaggcgaga acccatccgt cccgcagcac cagtaataac
    19501 aaccttgatc atggcaacaa cctttaccag cagcagaccg actacaccag cccggcctct
    19561 ttcatcacat gcagaagttt ttctctattg tcggttccca tcgggcacag cggcagccgc
    19621 atctcacggt cgatcttgcc catcatatgc agcgcttctt tcacaggaat ggggttggtt
    19681 tcataaaaga gggccgtaaa cagcggatag agccgatagt gcatcgcccg cgcatcatca
    19741 actcgaccgg ctaaaaatgc attgaccaac tcggccatat cactgggcac cagattggcc
    19801 gtcaccgtga tcactccctt gccacccact gccatcatcg gcagggtcaa cgcgtcatct
    19861 cccgccagca cggtcagacg ctccccgcaa agctgtatga tttcggatgc ctgctgaacc
    19921 gagccgcttc cttctttaat ggcaacaatc gttgggcaca ccgtcaatcg tgagatggtg
    19981 cttggcatca tatttacgcc ggttcgccca ggaatgttat agagaacaag gggtaaatcg
    20041 actgcctccg cgaccgcctt gtagtgcaga aacagccctt cctgcgtagg cttattgtaa
    20101 taaggcgtga tcagcagtgc gccaccggcc cccgccgctt tcgcgtgctt ggtcagagca
    20161 atcgcttcct gagtgctatt tgatcccgtt cccgcgatca ccgggactcg tcgtctggca
    20221 acttcgaccg taaacgccac cacacgatcg tgctcggcat gtgtcaacgt ggcagactcg
    20281 ccggtagtgc cgcaggggac aatcccttgc gtaccgctgg taatctgcca ttcgatgagg
    20341 tccccgaggg acttttcatc gagcttaccg ttcttaaacg gcgtcacaat ggcgaccaga
    20401 gatcctgtga acatgggagg ctccataagg aagacagtaa atactgaaat ctaggaaaga
    20461 aacgccggta tctgctcgcc atgcaccagg tcgtcgtaac tctctcgcgc tcgaattata
    20521 tggatctgtc cctcatgaac cagcacctcc ggcacacgag gtcgcgaatt ataattcgag
    20581 gacatgacaa acccataggc gcccgcactc atcacggcca tgagatctcc ggcattcatt
    20641 tccggcatca cccgatcctt cgccaaaaaa tcgccggact cgcacacggg cccgaccaca
    20701 tccacagtct gcttcgccgc acgcgcgacg ttttcataga ccggccttat atcatgatgt
    20761 gcatcgtaca ggctggggcg aatcaggtcg ttcatcgccg catccacgat cagaaaccgt
    20821 ttggtttcgc cgtccttcgt gtacaacacc ttcgtgagca acacgccggc attcccgaca
    20881 atcacgcgac caggctccat gatcaggata cacttcaggt cacgcacaag cgggaaaatc
    20941 gccgccgcca aatctttcgg ttccggaggg gtttcatctg aatacgtaat ccccaggcca
    21001 ccgccgatgt tcagataacg aataggaatt ccctgctcgg ccagggtctg caccatcgcc
    21061 aacactttct tcagcgactc gacgaagggg gccacttgcg tcaattgcga cccgatatgt
    21121 gcgtgcaaac ccaccacctc gatgtggctg aacgttgcgg ctgccttgaa ctcttcgatg
    21181 gcacgatccg ccgcgatccc gaatttgctc ttcttcaaac ccgtggaaat atagggatgc
    21241 gttttgggat cgatgtccgg attgatgcgc aacgccacgc gcgccttgac gcccatggac
    21301 gccgcgacat cattgatggc ctgcagctcc gcagacgatt ccacattgaa catgagaata
    21361 tccgccttca aggcgtcgcg gatttcctcc gcttttttcc caacgccggc gaacacgatc
    21421 ttggcgggag acacccctgc tttcagcgca cgaaatagtt ctccgcccga cacgatatcg
    21481 acgccactcc cttccttggc catcagacga agcaccgcca aattggaatt ggccttcatg
    21541 gcaaaggcga caatgtgagg aatgttctga aaggcactat cgtagacacg gaagtgccgg
    21601 acgagtgtgt ggtggctata cacgtaacaa ggggtcccca cctcttttgc aatgcgcgag
    21661 agcggcacat cctcgcaata gagctcacct tcccgatact gaaagtcatt catggtggtc
    21721 atcctcgatc aacaattcta tacccgcctc ggagaatccg tccaatagcc acaacgccaa
    21781 ccggtcgaaa ccacgaccac aacctcgact cacgtctatc gcgtgcccat cgtcctcagt
    21841 ggcggagtcg gcaacgggtc gggatccgga ggaatcgggg cttcttcaac ggtcactgca
    21901 ctcggcgcgc tgggcctgga agcagatccc cacacattcg cgcctggcgc taacagccct
    21961 tcacgtctca actgccgctc aatcaccggt gccacaccga cgtcttcagg ggggatcggc
    22021 gcacccaata ccccgcatcc gcccagtccc acaacaatcc acactgcacc gacggcacca
    22081 cccctgactc ccgggccaca ggccctgacg agcctcatcc cttcaacgcc ttctcaagct
    22141 ccttgatccg tgcctccaca cgccgtctcg ccgtgccgcc gatctgacct ttccggtcga
    22201 tggcgccccg cacggtgagg caactcagga cgtcctcacc gaaatgggcg gagaaacctc
    22261 gcaagtcttt caaggtcaac tgctgaaggg gccgctgctg ctcgagtgag aatcgcacaa
    22321 tctggccggt aatggaatgg gcctcccgga acgggacccc cttcgtcacc aggtagtccg
    22381 ccaactccgt cgccagcatt ccgccacctt cggccgcttc agccaggacg gtcttattga
    22441 ccaccagcct ccgcatcaac tccgtgcaaa gcgccaacga ttgccccgtc gtatccaccg
    22501 cgtcgaacaa cgcttcttta tcttcctgaa gatcgcgatt gtaactcagt ggcaatccct
    22561 tcagcagcgt cagggtaccc atcaagtgtc catagactcg acccgttttc ccacgcacca
    22621 actccggcac gtccggattc ttcttttgcg gcatcatact gctgccggtg cagaaggtat
    22681 cgggcagatc cacgtaacga aattcctgcg acgcccagag aatcaattct tcgctcaagc
    22741 gtgagaggtg catcataatc agcgacaagg cactgagtgt ctccaccacc gcgtcacgat
    22801 ccgagacggc atcgaggcta ttctgcgtca ccgcagggaa ttccagcagt gcagctgtat
    22861 atcgacggtc gaccgggtag ttcgatcccg ccaacgcccc cgaccccagc ggcatcacat
    22921 cgagccgttg ccgacagtcg tgaagccgac cccggtcacg atcgaacatt tctacatagg
    22981 ccaggaaatg atgcgccagc agcaccggct gagcacgctg caaatgtgta tacccgggca
    23041 tgacgacatc cagatgcgcc cgggcctgcc ccacgaggac tcgacggaat tcctgcaatt
    23101 gacccatcag cgtcgaaagc acatcgcgca aaaatagacg cagatccaga gccacctgat
    23161 cgttgcggct tcgacccgtg tgcagctttc cacccagcgg gccgatcaac tcagtcaacc
    23221 ggcgctcgat cgccatatgg atatcttcat cctgtggcga gaagggaaac cgcccgccat
    23281 ccaactccac cttgacgaat tgcagtcccc gcacgagctg cgccgactca cgggcagtga
    23341 gcacccccgc gcgctcgagc gtcctgcaat gggcgatgct gccttgaatg tcgtagggat
    23401 agagccgacg atcacaggcc agggaggagg tgaactgctc caccaaccga tcggtttgtt
    23461 cggcaaaccg gccgccccag gccttgcctt tgggcaaggc acgacgggac cgttgcggag
    23521 cagtgacaga tttgcgggca cgcgcgccgg cagattgggg cttcgccatt accgcgtcga
    23581 cctcttcttg cgctgggccc gaatcgccaa ccggagcgcg ctcagacgaa taaacccttc
    23641 cgcatccttc tgacgataca cgtcgtcttc ttcgaacgtc gccatgtcca gccgatagag
    23701 cgagcgcgga gacttccgcc caaccacggt gcaggtccct ttgtagagct tcacccgaac
    23761 cgtgcccgtg acatcccgct gggcttcatc gagcgccacc tggagcattt cccgctccgg
    23821 agcataccaa tatccgtagt aaatgagctc ggcatagcgg gggatcagac tatcccgcag
    23881 atgcagcact tcccggtcca tcgtgagtga ctccaggcct cgatgggccg catgcagaat
    23941 cgtgcctccg ggcgtttcgt acactccgcg tgatttcatc ccgacatagc ggttttcgac
    24001 gagatcgacg cgaccgacac catgctcgcc gcccaattta ttgagatggg ccagcaaggc
    24061 ggccgggctc atcttcttgc cgtccaccgc caccggattg cccgccacat agtcgatctc
    24121 gacttctcgg gccttgttcg gcgcccgctc cggcgagacc gacatcacaa aaatctcttc
    24181 agggggggcc ttccagggat cctccaaaat gccgccctca taactcgtgt ggaacaggtt
    24241 catgtccatg ctgtagggtt tcgccttagt ggccgtgacc ggaatgccgt gcttctccgc
    24301 gtactcgatc aactcacgcc gcgagcgcat ggtccattcc cgccagggcg caatgatctt
    24361 gatctgcgga tgcaacgcca tataggtcaa ttcgaagcgg acctgatcgt tacccttgcc
    24421 ggtcgcgccg tgacagaccg cctcggcgcc ttcatcggcc gcaatttcaa tttggcgccg
    24481 cgcaatcaag ggacgggcga tcgaggtgcc cagcaggtag ctaccttcat agatcgcatt
    24541 cccgcgcaac attgggaaca catggtcctt gacgaacacc tcgcggagat cctccacgta
    24601 gactttcttc acgcccaggg actgcgcctt ctttttgatg gccttcagat cttcgccctg
    24661 ccccaggtcg gcacaaaacg cgatgacttc gcagccatag acctcttcca gccacttcag
    24721 aatcaccgac gtgtccaggc cccccgaata ggccaatacc acttttttgt acgatgaccg
    24781 actcatacga ctccctttca cttacgtgct ccgtacaggt ttctttccga ggagcctgac
    24841 cagaatggcc ttctgcatgt gcaatcggtt ctcggcttga tcgaacacca ccgactgcgg
    24901 tccatccaac acctcggcac tgatttcctc accgcgatga gccggcagac aatgcatgac
    24961 cagcgcatcg ggcttcgcgc atttcaatag tcgcgcgttg agctgatagg gcgccagaat
    25021 cttgagacgc ttggcttgct cccgctcctg tcccatgctg atccagacgt cggtatacac
    25081 cacatccgca tctttcacgg ccacaaacgg atcggcgccg atttcaatca ccgcaccggt
    25141 ctgccgggct tcctcccggg ctcgatccac aatgccccga tccggctgat atccgagcgg
    25201 gcaaccgacg gcgatcgtca tccccatctt cgccgccgcc tcgatcagag aattcgtcac
    25261 gttgttgccg tcgccgacat acgcgatctt gatgcctttc agccgccgtt tcttttcctg
    25321 aatggtcaac aggtcggaca gggcctggca ggggtgattc aagtcggtca gcccgttgat
    25381 gacgggaatg ctcgcctcgc gggcccattc ttcggcaatc gcgtggtcaa aggtgcgcag
    25441 gacaatcgcg tccagatacc gagacaggac atgcgccgtg tcggcgacac tctcgccgcg
    25501 cgacaactgg atatcgccca tcggcaacac catcgcctgc ccaccgagct gattcatccc
    25561 ggcctcgaac gacacccgcg tccgggtcga aggcttctga aacagcaggc ccaacatgcg
    25621 gccctgcagc agaggatggg acaccccgcg acgctgcttc actttcagtt gcgcagccag
    25681 gcgcaacaac cccgtgagct ctgcgacggg gatcgagagc agttccagaa aatctttccc
    25741 gagaccggcc cgggaggatg accgatgcgg acgccgcgac atacgcgccc ctacaggatt
    25801 aatggtggct tgatgtagtt cgctgactca acacctgcgc aagcgcgacc agcaagcgat
    25861 cgatctcgcg ctccgtgatg atgagtgggg gaacgaagcg cagaacccga tcgccggtgc
    25921 aattgatcaa caatccgcgc gccaggcaat cggcgacaac agctttccca tcgatatcca
    25981 attccacgcc ttgcaacaga ccaagtccgc gcacgtcctt cacgcaacga tgccggtcct
    26041 tcagaacagc caaccctttg gccaacgtct cacccatgcg ccgtccctgg tccagaatct
    26101 tcccttccag cagcactcgc aataccgcca gcgcggccgc acaggccaga ggattaccgc
    26161 caaaggtcga ggcgtgggtt ccaggagaaa aggcctgcgc cacagtttcc gtcgctagac
    26221 aggccccgat cgggacacca ccgcccagcc ccttcgccag ggtcatgatg tccggctgca
    26281 taccgaactg ctcgtagcaa aaaagcgtgc ccgtgcgccc catgccggtt tgcacttcat
    26341 cgaaaatcag caacacatcg cgctcccggc agaggtcgcg caagccctgc atatagcccc
    26401 gatcggccac atgcactccg ccttcagcct gaatgggctc caacaggacg gcggcggttt
    26461 tcgatgtgat cgcgcgctcg acttccgaga gatcgttgaa cgtcacatgc gaaaaccccg
    26521 gcatgagcgg agcgaatccc ttctgcacct tctcctgacc ggtggcggtc aaggtggcca
    26581 tggtgcgccc gtgaaaggag ttcgtcatcg tgacgatctc gtagcggtct gctccgtact
    26641 tatcgtacga atactttcgg gccaacttga tcgccgcttc gttcgcttcc gcgccgctgt
    26701 tacagaagaa gactttctgc gcaaacgaat gttcgaccag ggtctgagca agccgcacct
    26761 gcggctcggt ataataaagg ttcgatgtgt ggatcaggtg ctgcacctgt ttttgaatcg
    26821 ccagcaccag gtccggatgt ccatgaccga ggagattgac cgcaatgccg gccacaaagt
    26881 cgatgtactc ccggccttcc aggtcataga ccttcgagcc acgtccccgc acgatcgaaa
    26941 tcggctggcg cgtataggtg ttcatcaagt actgttcggc attgagacgc aactcacccg
    27001 tcggcatggc acacctctcc catcgtgagt cggcgaagct accatagggc acaggtgaaa
    27061 gcaaccgaac gcacgcctgt cagcagctcc tggcgcggcc ggcggtctgt ttcgaaccct
    27121 gccgcttacg gagggccgcc cttgacgggg agagacggcg gatgggataa ggtgaccgta
    27181 tgaaatcctg ttcacaatgc cgacaagaaa accgggacga tgcccgcttt tgccaccaat
    27241 gcggcggcgc cttcgccgtg gaaagccggg agacagtagt ggaggccgac tcggcacctt
    27301 cgccgcagac ggatgcagag ctctggaaag ccttcatcgg ccccaatgcc gaccgctacc
    27361 tggagacctt caagaaattc acggggccgt ccgggcccaa attcgccttg acctggcact
    27421 ggccggcctt cgtctttgag cccttcctct ggtttctcta ccggaagatg tacgtctacg
    27481 cgctgatcta tgccatcggc ccggcgatgg cgttctatat cacccaggac ctctccgccg
    27541 acatcatctg gcgtgtcatt gcaggcgcca gcgctaacta tatctatttc tggcatgcaa
    27601 aagagcagct cgccaagatc aaaggcgaac gggccacggg tggagaagcc agacagcaga
    27661 tgttgggaga attaggcggc gtgcagccgt acgtcgtgtg ggtcggcgtc ggcctcctgg
    27721 tcctgaagat cggcctggtc gtcgccatgt tcaaggacgg accgcctgac gggtcgaaga
    27781 atcctcccgc caaaccccac cccgccagcg tcacgcacgt ctagcaaaac aacgttcaca
    27841 agtcggctcc agctttctga caacagctgt cacaaatctg aggcgcctat ttcaggcggg
    27901 ataagaagct caacggatct tttacaaact gaaaggcagt ggattcccgg atttcatctt
    27961 tcaaagccga caaaccaggc tctatcacct tcccgccttc actgatgagc cagcactgaa
    28021 gatcgcgtga aaactgcact gcagtctcca gcaactcaat cgttgtctcc ctcaagtcaa
    28081 aacggaattg gacttctaca agagactcac cctctcgcaa aatcgttaaa cacgacgtct
    28141 caaggctacc aagcacctca agatccttcg accatgtctt accagacgga agcatctttt
    28201 ccacatcgcg caacagcgta aggggtagcc gctgatccct ccaccacaca acggactctc
    28261 gctgttcctc agtgagaacc attcctgtac gcaacgagtt cgcctctgcg accgccttcg
    28321 gcactaactc gatttgaaat tgccagacag ccattaccta atccgaaccc tcggggcgga
    28381 atccaccctc cagctcgact catgaaacac cggccttcgc tattcggaca gacgctgccg
    28441 ctgccactca atccaggaca cgaaccagtc aaagtcctgc tggtaggcgg ccccgctgag
    28501 atcaggcgcg gcctgggtct gctccagttg cgtgtaggtg cgcggccagt tcttttgcca
    28561 ccaggacttg agctcgtccg ggccgtaggg ctgcccgttg ggattggtga tgccggctgc
    28621 cgcgcagagc ccggccacaa gcggccagta gcgatccttg ccaagaccga cgctccggaa
    28681 atgttcgcgg agcaggaaga ccacccagag ttcggcgctg ttcttcttgt tatgcttgaa
    28741 aggctgcgtt tgtcgcagtg gaaccgggtc gaaggttttg atgaccgacg tgtaatccgg
    28801 acctccgtag ctccccgccg actcggcaat cccctgcagc atgttggcga gttccacctc
    28861 gacgaccggc gagcgggccg ggctgtggtc agggcctccg gtactcaacg gattggccac
    28921 cgtcagcaat tcgatcaacg gcttgagctc acgcaaccga acggcggcgg ctttgagaat
    28981 ctgctcggac tcgagccagt aatccggatc gcccttggag gcccgttccc gaccgggcgg
    29041 cggcaacacc acgggaatcc agtagcgcac cagcacaaag aggacgtatt caacctcgcc
    29101 accggcctgc gcgacaccag ccagcgcctg actgaccccg agcgcccgcc gaaaaaaggc
    29161 ttcgaccctg tcttcaacct gcaggcgccg tcccattgcg cgccaccact cctgcaatgg
    29221 ggtccagtct gagggggaac gttccgtcat cgatgcgctc cggcgtgagg agaaaccggc
    29281 ggcatggtac tggctgcctc atctgattgc aactcagcat gacggagcca cggcgcactt
    29341 ccgtgggctc atcccgcttg acagggcgaa ggggaaatcg cataagctgt cctgaaaccg
    29401 ttctatcctt tcggacaagg agacgaggca cgatgagcaa attggttctc attcgtcatg
    29461 gcgagtcgca gtggaatctt gaaaaccgct tcaccggctg ggtggatgtc cccctctccc
    29521 cgaaaggtat cgaagaggcg aaggcggcag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29641 nnnnnnnnnn ntacgatgta cggccaccgg gcggagaaag cttgaaagac acagcggagc
    29701 gggtgctccc ctactacgac agccgcatca agccctacgt gctcaaagga gaaacgatcc
    29761 tcatcgccgc ccatggaaac agcctgcgag cgctggtgat gcagctcgag cagctgaccc
    29821 gggagcaggt cctggaactc aacattccaa ccggagcccc gctgctctat gagctcgaca
    29881 acaacgggaa ggtgctgtca caccggtatc tctaacagga aacaggaggg cgcggccgac
    29941 agcctacacg cactggctca gcaactcctg gtacttatcc aagggcacca gatcaggcag
    30001 gagcacgagc aacttcggcc gatcctccgg ttgaatcaaa cattgatttt cgagcagatc
    30061 tgagatttcc tcggaggcgg ccatggcgcc gccttcgtaa tcccccaccg gcgcatagag
    30121 tgcgcgccgt tcttccattt cgaccaggta gtcctcccac ttcaccgact caagcttgaa
    30181 cgtcgcagcc caatccgcct gaatcaattc cagcacccgc tgaaacagga cctcgcggta
    30241 cttccgtgga atgtgggcat tgacggccgc atgaacccag tagagcgtga aagagaggag
    30301 ctctcgcgtc atcgtctcct catgttcttc ctggccctcg atgccatatt cgctcaacag
    30361 ctcaacggaa acccggcgtg gaatcagttt gtacaaggcg tcggcaactt cggccggcgt
    30421 catggctcct tttggcatac tcatcctccc gaaaggggtc ccagcatact ggagccccgc
    30481 gcgcattgac aaccctggac ggtgaactgc cacgcggcgt cgattgcttg tccgccggaa
    30541 aggcctatgc tacatagagc ctcatcaccg tgagatggag caccatgaca cctatgcgac
    30601 ctgttcgagc gctgttcatc ggcctcgcct tcctcggcat cctctgggcc gcaccgacca
    30661 cagcagcaga ggtgcaggag ccacgctggg gcttcagcac cgatcttggc ctctggagcg
    30721 gaaccaccaa cgacaccacc ttcgcactcg gcttcgggct cgattactac atggaccaga
    30781 atttttcgtt cggcgggatg gcgctcttca cgccggtcgg ggacctgaca cagatcggca
    30841 tcgcgggagt cgcgaagtat catcttcgcc tgaatagcgg attcaatctg gtgccgttcg
    30901 ccggactagg cattctccac gcggacctca atcgaggcag cggcccgacc aaagtcgatc
    30961 gcaacgacac gagccatttc attcccctgg gcatgtcgct ggaatatcag gtcgggccaa
    31021 aaatcgcctt ctcgaccacc ctcatggtaa acctgcacca catcaccctc tcggccccgg
    31081 tccctgacga caacaccagc gtggcactcc tcttcggtat ccgctggggg ccgtaacgag
    31141 gccacagtga acgacagctc cggcgaactg cactgagcat gtcgaaaatt tgagtaccgc
    31201 gagaacgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg gcttgggcgg
    31321 ggcgccaccg caaccccaca tgcaacaatt cctgcagcag gtctttgtag gcgaggtcag
    31381 ctttttcggc cgagtcggca aaatcttcgc cggtggcgat ctgaggattg ggattcgctt
    31441 cgagcacgta gacgttaccg tctttgtcca tccgcatatc gatgcgagcg taaccactca
    31501 gccccaatgc acgatagacg cgcttggcca ggtgctgaat atggtcgacg actccgtccg
    31561 ggaggttcct cgcttcgcac gagcagatgc cgtacttgtc ctggtacttg cggctccact
    31621 tcactcgctc cgtcgcgatg cggcgggcct catccggcat cttgtccatc acgagttccc
    31681 agaccggaaa cacttgcagg tgcgcattgc ccatcacccc gacatagagc tcgcgtcctt
    31741 cgatgtaccg ctcgatgagg gctccagtgc cgacattgtc gtggatgaat gccacccgtt
    31801 cccgcaactt ctcatcgtca tcgacaatcg aggcctgcga aatccccagc gatgcctctt
    31861 cgctgatcga cttcacgatc aatgggaagg gcaggtattt gggtcgccgc accatgcgat
    31921 gcaggggcac gaccatgaat tcagggtatg gaatacgatg gtacgacatc acctgcttcg
    31981 ccagcgcctt gtcgcgcgca agcatgaggc cgcgtggatt gcagccggtg tagggaatgc
    32041 gcatcaattc gagataggac accacgttct ggtcgtagac cgcccgcccg tcgaactcct
    32101 ccagtaaatt gaacgcaatg tggggcttcc aatcctcgac ggccgcacga atcacttcca
    32161 gatcgctttt gaccccgagc ggctggacat catggccgag tttccggagg gtggagacca
    32221 catcgtactc agtcttccag gccgcgcctt caaggtcctg cccgttcagt tgatcgggag
    32281 gcacaagatc cttgtgcatc aaaacaagca cgcgcaatcg tctcatagtg ccaccttgtg
    32341 ccggccgctg tgcagatagt tcatggtctg caccgtcagg agaatcgcaa aatccagctt
    32401 ggcctgctct ttatgctggg tcaggtgtaa tttcgacgcc tgacatcgtt cgatcatgcc
    32461 ttccaacacc tgatcgatgg tgtactgata ttcgccggtc cattcnnnnn nnnnnnnnnn
    32521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32581 nnnnnnnnnn nnnnnnnnnn nnnnncggtc catgaaatcc agcttcttca tcaccggcca
    32641 gcctcgatac cgttccttcc agagcgagtg cggatcgagc cacacggcaa aggtttccgc
    32701 gaagtcttca tccggatggc tctgggcata ccagacatcc aggtggcgca cgaaactccg
    32761 gctatagggg cgcggcgtgt aatactcggg gtagggctgg gacgaacgtc cgaagagttt
    32821 ctggcgacga cgacgacgac gaagcaggta ggcgttttca atggcatggc cggcctcgtg
    32881 ccggaggatc cgcatgcacc agtccctggt gccgccttcc acttccaaca tctgactcgc
    32941 ttccagtttt gccagccggg gatgcgcaag atagaacggc acggcaattc cgggcacacc
    33001 atccggcgtg aaccattcgt ccgagatcca gaagtgtggc cggaaggtga gcctgcgcgt
    33061 ctccaactca cgagcgagtt gtgcaatggg ctcttggtag aaggtccctt cgaggcgcag
    33121 gtccagatcg cacatgcgca gatcgaggag ttgttcgtcg gtccactccg cccagaccgg
    33181 aacaggatcg gtcgcgccga tcccttgtcc gcgatgattc tttgtccgcc ccatcgttca
    33241 gccaccaaaa agccccgttc ggcgccgttt tgttctgttt caactatagc agctcatgga
    33301 aattatgcag ggcggtctcc ttcgaccaac gatgcaccac tcagggaaaa cccgcggacg
    33361 gtgagcgatg cgaaccggcc acttctgttt ggactacaga agcggtcggt tacggattac
    33421 agggcgggct cacgattgag cagatgggga actccgcccc agatgtggtc gatgacaaac
    33481 tgcagatagt cacgcgattg atccggttgt tcccgccagt cggggatgat ctcatgcacg
    33541 tccagaatcg gctgatcgat tccgtaacat acgtgattga agagagggac ttctcgaccg
    33601 aattccccgc gaatccacgc ctgatggttc ctgcccatgg caatgacgac ggcggtctcc
    33661 tcgatcaagt cgcgcgtcag cttccgttga cggtggcggg acggatcgac ccccttctca
    33721 cgaaggccgg ccaggaccac gggatggatc tcctgcggta acgcctcgat gccggcggat
    33781 ccgatcaggt acccgacccg cgagccgagc tgagccttca acgcatattc ggccacgagg
    33841 cttctgaaaa tattgcccga gcacacgaac agaatcgacg tcatcgcctc agcctgtcag
    33901 tctgaatgcc cctgttcacg gctatcccaa tcacggcccc ggttgcgcta caatgccggg
    33961 ccatgcagag cacaaccacc ccaccatccc caggcaccgc acagccgacc aggcactcca
    34021 acgagatcga acgcgtgcac acccgcctct gccgcctggt gggacaagcc attgccgact
    34081 accgcatgat cgaggaaggc gacaaggtca tggtctgcct ttccggcggg aaagacagtt
    34141 acgggctcct ggagatcctg ctgtcgatcc gcagccgtgc gccgatccat ttcgacatca
    34201 ttgccgtcaa cctggatcag aagcaaccgg ggttccccgc ccatgtcctt ccggaatacc
    34261 tgaccaggcg cggcgtgccc ttccatatcg aaacgcgcga tacctactcc gtcgtcaaac
    34321 gcctcatccc cgaaggccag accacctgct cactctgctc gcgcctccgt cgcggccatc
    34381 tctaccggct ggccacggaa ctgggcgcca ccaagattgc ccttggccat caccgcaatg
    34441 acattctgga aacgctgctg ctgaatctgt tgttcaccgg caagatgaag gccatgccgc
    34501 ccaagctgcg ttcgaaaagc ggccgccatg tagtcatccg ccccctggcc tatgtgaagg
    34561 aagccgatct ggcgcggtac gccgccttgc tccagtttcc gatcatcccc tgcgacctct
    34621 gcggctcaca ggaggacctc aagcggaagc aagtcaagac cttactgcag gactgggatc
    34681 aacggttccc cggttccaac gacagcatgt tcgccgccct cggcaatatc gccccgtcgc
    34741 tgctgttgga tcgaacccta ttcgacttct catccctcaa ggcagatgcc tccccaacag
    34801 aaccagccgc gagtgattct gaagacgatt tgctctagcc cgacgcgctg ctgcctatag
    34861 caggctgtgg gaaaacccgg ttggcatgcg aacactcaga tggtccacat cgagtggcat
    34921 agcagaagaa taccccgcaa gatgcgcaaa atggccgtcc agcgttgagc ctctatgcga
    34981 tgcgagaacg ccgttggcgg aatttttccg catcctgcta gacagaccgg cggatgcgaa
    35041 acatctctag aagggattgc cggagcagta ccggaagtca gacacgctgg cggagatggg
    35101 tgcgacggcc gcatcgcaaa catcgataag gatagtagcc gatcaggaag aagaaaaaat
    35161 ccaacaggcc ttggcggcgg gaacgtttcg tataccctga acatttgccg caatacgaca
    35221 tcgtacactc tcacttgttg aacatccggt ccactgcttc caacggccgg gattggaagg
    35281 aaatatccta ctgaaacatc ctagggccat gcaagtctag tttgcacgac gtgtccgtcg
    35341 tcactcggcc acgggagtcg tctcaacgtg acacaattgc tgtcggtcga tcaggtgcgc
    35401 agacgtgcca ggcaaggaag tgtgccggac tcagaccggc taggttcgac ggcggagacg
    35461 aaaccggtag acgcaaatgg tgcagcgaaa tggatagaac ccgatgagat gcataaaaaa
    35521 atcccgccaa ccatgacggg tcactcgctt ggcctcacct gaacacattg gacaatagac
    35581 catggactgt tgcggcgcgc ctttctccgt cgcatcgggt aagatgaccg aggtgcgcgg
    35641 ggacgtgagt gtgaatcagt ttgctacggg gacgcaagtc ccatcgaagg aggccggccg
    35701 gcgacatgaa gcggtccaca ccatcccgcc ggcctgcagc cgagcccccg ctgtggtcga
    35761 tcgcggccgc agccggcgtg atcatcctca ccccgatgtt gctgtattcc cttgcgccgg
    35821 aagggccgat tcgcgaaggt gataccgtct tctccagcgg agcccacaag gtatcgctct
    35881 ttgaacctga ccgctaccgg caggccggct acgaggcgac ctgcatgctc gatccgaaag
    35941 acccgctcat tgtgacccat tccccctcag aagatgagtc gggcgaggtg atcatcgccc
    36001 aggtccaagg caaaagcacc atcgagtggc cgttttgccc gccgcaagcg gaacttctcg
    36061 tcaaacgaca ccacatcacc caacagccca gcatccttca ggacatacgt gacggactgc
    36121 tgcgcctgtt caaaccttcg tagcgacaga accctccccg cccggcgtta cgccttgagc
    36181 aacgacgcat agtgctgacg aaacttggcc actttgggtc cgacgacgta ggcgcagtag
    36241 ccctcaaacg gattccgtgc aaaatactcc tggtgatagg cttcggcctc ataccaccgg
    36301 gtcgccggca ccacttcggt cacaatcgga ttgggataga cgcgctcccg cgtgacggca
    36361 gcaatcacct cctgagcaat ctgctgctgc tccggggaat ggtaaaaaat tccggatcga
    36421 tactgcgtgc cgatatcgtt gccctgccga ttgcgcgtcg tcggatcatg aatcacgaaa
    36481 aatacgttca agagatcacg atacgtcacc aaccgcggat cgaacgtgat ccgcacggcc
    36541 tccgcatgcc ccgtgtgccc accacacacc tgctcatatg tgggggcatc cacctgcccg
    36601 ccgatatatc ccgactcgac cgaccgcacc ccgttcacct ggtcgtatac ggcttcaaga
    36661 caccagaaac agccccctgc caatgtggcg atgtccgtcg tttcctgccc catgtcccac
    36721 ctcgtcgttg actcgcaccg gcaggctagg cctgccctcc attccaccac tcccgccctt
    36781 cacgcttcaa caaagcctct gcctccggtg gcccccagct tccactcggg taattcggga
    36841 acgacgccgg tccgggaagg gacttccaca cgtcgagaat cgtttgcacg acagcccagc
    36901 ccagttcgat attgtccgcg cgctggaaga gcgtcgcatc cccggccatg gcatcgtgca
    36961 agagggtctc ataaccggtc tggggggcgt tgccgaaata gtcggcatac tgaaagtcca
    37021 tgtccaccgt gccgatccgg accgtcggtc ccggcacctt ggcatcgaaa cgcagggaaa
    37081 tgccttcatc cggctggatg cggatcacga gcacgttcgg aaccaatcga tccacctgag
    37141 tttttctaaa caacatgaac ggcgcccgct taaactgcac cacaatctcc gtcaaccgcc
    37201 tggtcatccg cttcccggtc cggagataaa acgggacccc ggcccagcgc cagttatcga
    37261 tgaacagctt catcgccaca taggtctcgg tcatcgactc ggaccccaca tgcggctccg
    37321 accgataccc cgttaccgcc ttcccgtcgg acgtgcccgg gccatactgc ccgaacgaaa
    37381 caaaccgcag cacgtccagc gaactcagcg ggacaacccc gcgcaacacc ttggcctttt
    37441 cgtcgcgtac ggcatcggcg gcaaaggagt tgggcggttc catcgccaga aagcaaagca
    37501 gttgcagcag gtgattcggc accatatccc gcaacgcgcc ggcctgttca taatagcggc
    37561 cccgatgctc caccccgaga ctctccgcca cggtgatttg cacatggtcg atgtactggc
    37621 gattccagac cggctcgaag atcccgttgg caaaccggaa gaccagaata ttctgcaccg
    37681 tctctttccc gagatagtga tcgatccggt agatctgccg ttcttgcaag acccgttgga
    37741 gctcgtggtt cagcgcccgc gccgattcca ggtcatgtcc gaacggtttt tcgatgacga
    37801 cgcgacgcca tgtgccctca cgttcgaccg tcagaccata ctctcccaga tgcgtcacga
    37861 tttgtccgaa cagaccggga atggtggcca tgtaaaaaat atagttgccc tgggtacccg
    37921 aggacgcatc gacctgctgc agcaactcgt tcagccgtcg ataggtggcg ccctcttgga
    37981 aatccccggc caggtaatgc acgcgctccg acaacgcctg ccagaccgat cgatccacgg
    38041 tcgccggcag atagtcgccc atcatccgat ccaacttctc gcgaaactcc gccgtcgtca
    38101 tctcgggacg gtccaaccct acgatggcga acccctccgg caaaaaatgg ccggccatga
    38161 gattgtagac cgccggaatc agttttcgct tcgccaggtc gccatcgatc ccgaagatga
    38221 ccatgacgca aggagtgcgc agctggggga ggtccatcag aggctccgcg ctcatcgctc
    38281 tggccgttca atcagcatgg ggcatcctgc ctgatccctg ctacaaccgc aacctcctcc
    38341 gcgggctcat gacggttctg ccatttgagt aggcgcccgg cctgccccgt gatactcatg
    38401 gaagggacga cacacctgac aaccggacgg gcacctccaa catgccgctg ccatctcctc
    38461 cgtcacctgc tcctcctctc cgagtcccgc tccctggatt gaggcggtgg agtaccttcg
    38521 ccgcactctg cggcatgctc gccatgatgg ctggcgccgg attcgtgagc ggcacgacgc
    38581 ctcagctcac tccccgggac gtgcacatcg aagtgccggc cgatagtctg ttcggctccc
    38641 tgccggtcaa ccggggtctc acggtttcaa ttcatgtccc ggaaccaccg gtcagtcgcg
    38701 gagaaccact cgtcgccgtt tttgaaaccc cgtcctccgc accctacacc gtcccgttgg
    38761 aaatcgatcg ctcccggcat cactattccg ccaccgtcga cctcggtcgg ctgtcaggca
    38821 ccatgggcac tcccccgaaa gcgaccgccc ttcaggtgct catcggccgc cgacaggggt
    38881 tacacgtgga agctctcgtg cgccgtaccg tcgtcgtcac ccttgcgatt ccgggatacg
    38941 ccgatcaccg gtcagcccgg acggatttcg ccaaccagat ggccaatggt tcagggcctc
    39001 cccgaaacca accagccgac ctggcgttgc tggacggaca ggttgaagaa gaggaattgg
    39061 tcgggaacgg gggactgcca cagcaggaag gctattggaa gatgctgcag ggactgatcc
    39121 ataaagggat gccggatgga gcggccactc ggcgcgggag agagatcggc aggatgccgg
    39181 gcatcgggtt tcggctgtat gccaacggcg aggctcagtt gatcgaggtc gaacgtagtt
    39241 caggggattt ggaactcgat caggcggcgg tcctggccgt cgtcaacgcc cacccctttc
    39301 ctccgtttcc gcccggaacc agcgacaccc acgtcgatgt gcatgtcgag attcccggcc
    39361 ttccccgtta gaacggcacc gggtctcacc ggaccccgga cgcgctaatt gttgtgcgtc
    39421 cgcttcagcc agtcgataaa gcgctggagc ctcgtctgct cctcgtcgtg cacgtgcaca
    39481 aacaccagtc caaagcctgt ggcattcgtc cagcgcactt cggcgacctc gaccgtcatg
    39541 ggttcatacg gagccggaaa ctcgacgtgg agggagagac aggcgcgtgc agggagcgac
    39601 tcctcgctca cgacatggca accttcattg gacagacttg tgaccaaccc gcttccggca
    39661 atggcattac ccgaaaacgt gagcggcact tcgatttcat agcggggact atcgcgacgc
    39721 tccacatgtg cctttcaaaa gatcagggat ctggactcga tccataaaaa tgtccggaaa
    39781 tggatacgct gttccgctcc cgaaatcaac cgtgctgtcc agggtatcac gagcctgcgc
    39841 accggccctg ttgccgctcg aggcctggac gatcagtcgg tcggcgtatc gcttaggata
    39901 caaggccgta tctcttgtga cagcctgaat caccccgccc atcgttgaaa caaccagaca
    39961 cccttgaacc accggaatat ccagtccggc atgccaccca ctccttgaaa gccggttcct
    40021 gtttcggctg gcacgagggt tgctcgctac aagagtacca gtagtttgat cgacccgaac
    40081 gggaccctca gagggagcgt ctcatggagc acctatcgga gattgtgatt accgtcgtgg
    40141 ccttcgcagc cttgatcggg cagtggatga cagacctccg caccatgccg aacaggaacc
    40201 gccccacaag ccccgaagag caatgagact gcctccgtca ccatgcaagg actctgaggc
    40261 cgggacgata gaccatgcgc ttcgccaccc cgatcgcgcc ccctcgaaga cccaccggta
    40321 ggattctggc tgtcatcgcc cttgtcctca tcccgttccc tgtgagcatc cccctgtcgc
    40381 tgtggctgac aggccggcgc catttc
//
LOCUS       DAOQRM010000007        36471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_597_length_36471_cov_15.679042, whole genome shotgun sequence.
ACCESSION   DAOQRM010000007 DAOQRM010000000
VERSION     DAOQRM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 36471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36471
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_597_length_36471_cov_15.679042"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKK81_01420"
     CDS             <1..763
                     /locus_tag="PKK81_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV31554.1"
                     /translation="GSIEPALFWDTLDPYHYVRIHRQVSAREGEDSVLIVGGEDHKAG
                     QADDGEARYARLEAWARERFPMMNAVVFRWSGQVMESVDGLAFIGRNPGDTDNIYIAT
                     GDSGMGMTHGTIAGLLITDLIMKRESPWSSLYDPSRRPVRAAGEFIRESLNVAAQYTD
                     WVTGGDVETEAQIPHAQGAVLRDGLTKIAIYRDAQGELHKCSAVCPHLGCIVAWNHAE
                     NTWDCPCHGSRFDKFGKVLNGPATSDLSPLKQEDT"
     gene            963..2696
                     /locus_tag="PKK81_01425"
     CDS             963..2696
                     /locus_tag="PKK81_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV31555.1"
                     /translation="MRYLALAADYDGTLASAGTVDTDTIQAIERLVASGRKLILVTGR
                     VLPEILEIFPQISLCERVVAENGAVLYRPATKEITLLAPPPPPAFLEEVQRRKVEHLT
                     LGNSIVATRVPYETVILDIIRDLGLELRIIFNKGAVMVLSHGINKATGLTAALKQLEL
                     SPHNIAAIGDGENDHAMLTYSEYAVAVENAVPMLKETADRTTVGDHGHGVIELINELV
                     ENDLAVADRSVARHRIALGTQENGGDITFHPARQNLLLAGTSGSGKSTLATGLLERLG
                     ERGYQLCVIDPEGDYENFPQAIVLGTAQDGPSHAEILTALANPNNHVVVNLVGLPLQD
                     RPSFFLTLLPKLQELRSKSGRPHWMLVDETHHLLPVDGNPTTPGLMKDLAGMIYVTVH
                     PDHIEHSILKTVDIVFALGKSPDETLKQYCAAIQQPAPAATAARLQPGRAIMWNRASG
                     ETPFVLEIAPSTIERRRHRRKYAEGELPPEQSFYFRGPAGQLNLRAHNLLLFMQLGEG
                     VDQATWIHHLRSHDYSTWIKQVIKDEALAQRVHDVEQQAHLPAEESRQLIRSAIEERY
                     TVPAGGDEHTS"
     assembly_gap    3037..3136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3201..3638
                     /locus_tag="PKK81_01430"
     CDS             3201..3638
                     /locus_tag="PKK81_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31556.1"
                     /translation="MKTALHSLLALALVSLAACSHSPTASKTRSEMEAGTKTGMVKNI
                     VIKETIVPDALTVRAGDEVRWINQRQDSVTVTIDEPLEHNVSCRNGFSKAMGMGVDNS
                     TTLSSNETAGLCFSRIGTVKYSVRPADETKTSMANTHGSITVQ"
     gene            complement(3731..5926)
                     /locus_tag="PKK81_01435"
     CDS             complement(3731..5926)
                     /locus_tag="PKK81_01435"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNV31557.1"
                     /translation="MPKDRSKRENEKTAALDSAREKSGDHFLTTDQGLRINDDQNSLK
                     AGARGPTLLEDFILREKITHFDHERIPERVVHARGAAAHGYFQVYKSLASLTRAKFLQ
                     DPQVKTPVFVRFSTVAGSRGSSDLTRDARGFAVKFYTEEGNYDLVGNNIPVFFIQDAV
                     KFPDLVHAVKPEPHHEMPQAASAHDTFWDFISLMPESMHMIMWVMSDRAIPRSFRMME
                     GFGVHTFRFVNAKGVGRFVKFHWKPLLGVHSLLWDEAQKISGLDPDFHRRDLWEAIEA
                     GQYPEWELGVQIVEEKDEQKFEFDLLDPTKLIPEELVPVVRIGKMTLDRNPDNFFAET
                     EQVAFHPGHLVPGIDFTNDPLLQGRLFSYTDTQLIRLGGPNFHEIPINRPLAPLHNNQ
                     RDGHMRQTINSGPVSYQPNTLGGGCPMQAKADMGGFLSYAERLPGSKVRERSKSFFDH
                     FSQAALFWRSQSEPEQEHLVQALQFELGKVENLPIRERMVGMLSQIDKGLAGRVAEGL
                     GMPVKKIEGLLNLSIPADGDPKDFQPKRAMTSLDKSAALSMVLAPKEGIQTRKVAILV
                     ADGVDGEAVKGMQMALVAAGGQGKIVATRSGSLKAANGSELKIDFSLLTTGSVLFDAV
                     YIPGGEQSVSALLRTPKARTFVQDAYLHCKAIAATGEGVALFEAAVGAAYPEAEHGSK
                     TRQTPKDWSQDSGIVLAPDSSPKQIAARFIKAIEMHRHWAREIRLASPS"
     gene            complement(5962..6462)
                     /locus_tag="PKK81_01440"
     CDS             complement(5962..6462)
                     /locus_tag="PKK81_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV31558.1"
                     /translation="MARAAGFVSTVFEMLKEDHDKVKKLFEDFESAEGRERGEIAKTA
                     IRELEIHADLEERLIYPAIRAEVDEADMMNEAIEEHHVVHVLIAELKKLKPGDEKFQA
                     KFSVLSELVKHHIEEEEGEMFPKAEECDIDWESLEASVMKRKETLLAKASGRKTGARP
                     SRKTIR"
     gene            6672..6911
                     /locus_tag="PKK81_01445"
     CDS             6672..6911
                     /locus_tag="PKK81_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31559.1"
                     /translation="MTTPRFIPICCGCNNVRDDSRRDEREPGWGTLDAYLHTHHLTSG
                     DYRLTHTYCPSCSVRFSKLSGGHAVTPEVAASRSL"
     gene            complement(7052..7741)
                     /locus_tag="PKK81_01450"
     CDS             complement(7052..7741)
                     /locus_tag="PKK81_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNV31560.1"
                     /translation="MRLRVASYNIHRAIGRDGRYEPGRILRVAQELNADIIALQEVDF
                     PRQDHAPIAPWLAQKTGMIAIGGPVWSREGVGYGNALLSRYPVDRVRRWDLSVGRHEP
                     RGALDVELTICGRTVHVLNTHLGLWPRERPQQADRLLELLSLSRRDLTILMGDLNEWH
                     GWGKSLRGLRRVFGSPAAPVTFPARWPVLALDRIWIFPAQALLTVEAHDTPRSRLASD
                     HLPVKADIYLP"
     gene            complement(7763..9970)
                     /locus_tag="PKK81_01455"
     CDS             complement(7763..9970)
                     /locus_tag="PKK81_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNV31561.1"
                     /translation="MERAEVLKACQAQEQTPPSFLVRGRTCWRVERSDRCGFLIDGEA
                     YFKTFRDVALHAKHSIMIVGWDLDTQIELVRGPAEPSEFPSKLGEFVSALLRRKRKLR
                     VHVLNWDFAMIYALEREWMPTAQTGWSGHRRLSYQVDGQHPIGASHHQKLVVIDDTIA
                     FVGGLDLTKSRWDTPAHACQDPLRVNADGQPYAPFHDVQMMVSGEAAAALGELARDRW
                     FNATGRRLPLAIQRPVEELWPVALVPDVEPCGVGIMRTQPAYAGQPEVREIELAYLEG
                     IKRARRSIYIESQYFTSNAISRALAARLMEQDGPEVVLVLRHNCDGWLERQTMDALRS
                     RILHELELADHYGRLRIYAPTVPGTQGRDMVAVHSKLLIVDEDFVCLGSANVSNRSMG
                     FDTECNLAIESGGTSRLQAVIARLRNGLIGEHLGVEPELVAEHVQRLDSLGPAIDHLR
                     GGDRSLEDGCFDKSPAEAISIPEQLLIDPERPMAADEVLGAVVQHRERAHVGRRVFLG
                     YTVLLLLGLLAAAWRWSPLSEWMNVNDLVDAVRALGQGPTGSLVMLGGFLVGGFLAVP
                     ITVLIVVTILAFGPLVGAPSALAGALLSAVTIFGLGRALGRYRVQRFAGRRVAHLSRR
                     IAQRGFWAILIVRLLPIAPFSMVNLVAGATSLSLRDFLLGTALGMSPGIVLMSAFVDR
                     LAEALRNPSPVTFGVLGLLMAAAWSVAWYVSRRLQVRDTAPDVGNVQQVAPAA"
     gene            10659..11117
                     /locus_tag="PKK81_01460"
     CDS             10659..11117
                     /locus_tag="PKK81_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31562.1"
                     /translation="MLRTIIGVGLVSGALLTGWSPSWNEAPAPIPHPGPGPTVPPKPS
                     DPVPLPPDPKPVLPPHPANTPHAYLMVDPATDPQNTVSGVLEKVDLIAKQGLLKTDLG
                     REVAFQIPKPELFLNLSVGQRVTLKLDDRQQAVRVMDTTAPEIPPPFPAK"
     gene            11287..>12260
                     /locus_tag="PKK81_01465"
     CDS             11287..>12260
                     /locus_tag="PKK81_01465"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV31563.1"
                     /translation="MNSSDSVSWLTGSEMGGRIRTFDWRRTPLGPIEDWPAALVSILG
                     VCLTAQYPMAIYWGSEGWLLYNDAWRPILGDKHPWALGRAAHEVWPELWDTISPLLHS
                     VQTTGQAVWRGDELLPMQRFGYTEECYFDYSFNPIRGQNGAVEGILNIVQETTYRVLN
                     DRRMRLLRELASRSGFAHSQEDACNLAIEALATDQADVPFALLYHIDRDRRHAHLIAS
                     TGLPPEHPARQQTVSLTPEEPDSGWPLSAPLQEGVPVIVDDVGDRFGPLPGGSWSEPT
                     GQALLLPLSTVRWDGRAVILAAGINPRRPLDDDYRSFFTMVESHIAGAL"
     assembly_gap    12261..12360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12361..15756
                     /locus_tag="PKK81_01470"
     CDS             <12361..15756
                     /locus_tag="PKK81_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31564.1"
                     /translation="YVDRDMWEKIVLNLLSNAFKFTFQGQIALSLHAQTGGVELHVQD
                     TGVGIPPEALPRLFERFYRVQDMRSRTYEGSGIGLALVQELVQLHGGTVRVNSQVDQG
                     STFVVTLPFGNAHLPPDKVVPHGRPTTPTTGVGPYLEEALLWLSEPERESKPADGRPD
                     PAEAPLSTTSAETARPRVLVVDDNADMRQYIARLLADRYHVIAMPEGMSALIEARRQP
                     PDLILSDIMMPQLDGFGLLQALRDDPALKTIPVILLSARAGEESRIEGLGRGADDYLV
                     KPFGARELLARVATHLDMARVRREAQREIAHSEARFEAIVSQATAAVAQTDLTGRFLF
                     VNQRFCELLGYSASELLGMNMQQVTDPDDLPANLVLFHRLTAGGPDFVIEKRYRRKDG
                     SAVWTRVSVGGVREDGELRHILAVGLDITDRKRQEAELLALTHRQSLLYDLADVVNRA
                     EALTGLYEKAIDTIIEALHADRASILLFDDEGVMRFEAWRGLSQAYREAVEGHSPWIP
                     DQQDPPAIFIPDVAASDLHTSLRSVIQSEGVSALGFIPLTYGGRLLGKFMMYFDRPHV
                     MDEQEIGLAQTIARTLAVGIERARAGELLRSNEERLRLAMAAGSMGAWDADLSTGATD
                     WDAKQYELFDRPKDKPLTHAEQFYESIHPDDVPRVREAAAQAELTGSFSQEFRVIAQD
                     GSIRWLSGHGTAVKNDQGQAVRMVGINYDITRRKHVEEALQRSASDLEQRVVERTLEL
                     SQSREQLRALATELNLAGQRERQHLATELHDYLAQLLALSRIRLAQAKQQPMTTALGK
                     ILSELQDVMDQALTYTRTLVAQLSPPILKEFGLPMALKWLAEQMMQRDLQVSLELGTD
                     HLALPEDQAMLLFQSVRELLMNIVKHASAKQARITVREEEGWLHITVADQGRGFSPPD
                     SIPATRSSGISGFGLFSIRERMLALGGRFELQSHPGQGTEATLTAPLAPRQPRAKPQA
                     GADASVPGALSHKTAHAATVREGLADEREPVNRPIRVLIADDHAMVRQGLCGLLAAYG
                     NIQVVGEAADGEEAVTLTIQLQPDVVLMDVNMPQMDGIEATRRIKQVLPATIVIGLSV
                     QNAAHVGRVMREAGAAAFLNKEAAVEDLYQTIQVARKELLQVP"
     gene            15753..18872
                     /locus_tag="PKK81_01475"
     CDS             15753..18872
                     /locus_tag="PKK81_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31565.1"
                     /translation="MRRFQQSPNPLLSGAIVFSLATVFVLDFYTPLGITVWVLYLIPV
                     VLAYLFWFPVTPVAVAGAVTILTLAGLLLSAPGIDPSVSRINRLVGIITSWALAAFGA
                     HFIRNRIAVRKEEWIRAGQTLLSERMMGELALNSLGENLLHCLAKYLGAEAGAIFIDD
                     GRGFHRTATYGVPEGTAMPERFAAGDGLLGQALQHKHTVVVHDLPEGYFTIGSALGRS
                     APRHLMIAPLTVDGRVNSVLELGFFTRVTESDREFVDRVAESIAVAVRSARYRTRLQD
                     LLEETQRQAEELQVQSEELRVANEELEEQSRALKESHSRLEQQQAELEQTNSQLEEQA
                     QILEIQKEDLNQSTAILESQTRQLERASRYKSEFLANMSHEMRTPLNSTLILTKLLAD
                     NPQGNLTADQIKSLTTIESSGHDLLALIDDVLDLAKVEAGRIDLTPQPVPIPRLLDSL
                     RSIFKPLAEQRGLRLTMQQRPGTPDSFETDSQRLEQVLKNLLSNAIKFTEVGEVSLEV
                     SRLSDGRLAFTVQDSGIGIPQDQHELIFEPFCQADGTTSRKYGGTGLGLSISREFVRL
                     LGGDIHLTSTPGQGSSFTVLLPQRLSSAPFPAPARSLHPQSPPKPMRPGDIPPIPAIP
                     SESLVATTHIEDDREQLAANRRVILIVEDDEPFSKILADLAHELNFQVLVATTAADAL
                     ALASRYLPSAIILDIGLPDHSGLSVIDRLKADPRTRHIPIHVVSGHDYERTALSLGAV
                     GYMLKPVKREQLVEAFRQFETRLTDKPRRVLIVEDDPAQRESLRLLLGSDEVETIGAD
                     SAAECLAHLAERTFDCMVLDLTLPDASGFSLLETLSREEHLAFPPVIVYTGRELSPDE
                     EQRLRKYSSSIIIKGAKSPERLLDEVTLFLHQVVTALPPEQQRMLEKARSRNAVLESR
                     RILIVEDDVRNIFALSAVLEPHGAKIEIARNGRESLRILEESLQHDDRLIDLVLMDIM
                     MPEMDGLTAMRAIRERPEWDTLPIIALTAKAMKNDQEQALAAGANDYMAKPLDVDQLL
                     SLVRVWMPR"
     gene            18869..19708
                     /locus_tag="PKK81_01480"
     CDS             18869..19708
                     /locus_tag="PKK81_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNV31566.1"
                     /translation="MTSDTAMKTEDIELQLFLEAVYQRYHYDFRGYSKASIKRRLLLA
                     RERIGCTTFSGLQDRMLHDHAVLPQLLDYLTVQVSDMFRDPGYFRALREQILPHLKTY
                     PSLKVWVAGCSSGEELYSLAILFREEGLESKTMFYATDINAGALAKAEAGVYDLQRIA
                     LFTRNHRLAGGRGSLSDYYTAAYGAARFDKTLRRRTVFSDHSLVSDSVFAEVHLVSCR
                     NVLIYFDRPMQDRAIGLFKEALVRKGFLGIGARESLRFSAHVKAFTELLPGERIYQKR
                     GDE"
     gene            19708..20310
                     /locus_tag="PKK81_01485"
     CDS             19708..20310
                     /locus_tag="PKK81_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNV31567.1"
                     /translation="MALPLHGCLREPRAIVIGASAGAVTALSRLLPPLAADFPLAILV
                     TVHVPADKPNSIPALLRTKCRIAIKEAEDKEPIVPGTVYFAPPDYHLLVEQDRRLSLS
                     NEEPVNFSRPSVDVLFESAADAYGKDLLAIILSGANEDGARGARAVREAGGIVVVQSP
                     DSAEARMMPESALTACPKARAMNLHTLAELLCACGGTVQC"
     gene            20304..22385
                     /locus_tag="PKK81_01490"
     CDS             20304..22385
                     /locus_tag="PKK81_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31568.1"
                     /translation="MLTDSPAKFLLVDDHEPNLVALSAVLSRDDVEMLRARTGREALE
                     LLLQHDIALAVIDVQMPIMDGFELAELMRGSQRTQHVPIIFLTAGPQDNLRRFRGYQA
                     GAVDFLYKPIEPDVLRSKTGIFLDLYRQREELARQRDTFITLAEEKARLLRERDEANR
                     RLRESEMRFRTLADSAPVIIWMNGPAGCEFVNQTCLDFLGIDHLGQIDIFDWARHVHP
                     DDRDQAAAVYRQAVEGRTRFEASLRCRRRDGTYRWIRSVGMPNLSADGGVLGYIGASF
                     DVTESKEAEERLQRWSVDLERAVSQKTSELLQSQEQLRALATELNLTEQRERKRLATE
                     LHDYLAQLLVVVRMKLRQTIPLVTGERIPELLKDADHALTQSLDYTRSLVAELTPPTL
                     KEFGLLQALTWLATQMHRHGLTVTVRQDAAPLPLPEDQAVLLFQSVRELLFNVLKHAK
                     ATEATIVLTVRADGQLEVVVADDGCGFVTAPKNRPGTTANRFGLFSIQERMAAMGGQL
                     VIESVLGGGTRATLTTPYWNSREKPEDDDTLTPAESRAPSLRTRSTDVEHLSHSHHPT
                     SKIGILLVDDHAMVRQGLRSVLEHYDDVAVVGEASTGREAIEFVERLRPAVIVMDINM
                     PETNGIDATVEIKARHPDVAVIGLSVHNTAEARKAMLQAGATTLLSKESAVDELYAAI
                     RQALADEGVPR"
     gene            complement(22394..22777)
                     /locus_tag="PKK81_01495"
     CDS             complement(22394..22777)
                     /locus_tag="PKK81_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31569.1"
                     /translation="MKQTLGGEIDGMRLNDSRQASAQTTAGSLGSSRAVQQGPLIEVS
                     ESDGTFNVYADFRAMPEMASAEEIAVEFARQGIILSSGSHQRYVPIPTDAQIEHARVT
                     IDAGIVRMSVPTADLGHRWRSIVMW"
     gene            22992..24971
                     /locus_tag="PKK81_01500"
     CDS             22992..24971
                     /locus_tag="PKK81_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV31570.1"
                     /translation="MHSTDGDNPTASEIPLQIDLLLSRLAAIVESSDDAIISKDLNGC
                     IMSWNQGAQRLFGYTAQEMIGQPVHRLIPQDRLDEEPRILERLRLGQRIDHYETVRQR
                     KDGTMIDVSLSVSPIKDRGGRIVGASKVAREISERKQGERDLANLVEALPVAVYTTDA
                     EGRITRYNQAAVDLWGSRPVLGTTRWCGSWRVYWPDGRPMPLAACPLAQCLRLNRPIR
                     GLETIIERPDGTRVPVAPHPTPLRNAAGELVGGVDVLMDLTERKRAERQLQESAAELE
                     RRIKERTHELVLSQERLRALASELTLTEQRERRRLAVELHDYLAQLVVASRLRLSQAI
                     PKVQDETASAALTNVDGMLDQALTYTRSLVAELSPHILYQFGLSKSLVWLGERMKQHA
                     LEVTVKTGPTSFTLPDDQAVLLFQSVRELLFNIVKHAKTDRAVITINVDEQRELWICV
                     EDEGVGFDVTELTDGEDSRGKFGLLSIRERMETLGGECELSAAPGAGTVAILRLPLSG
                     SATASPSTPPTTVPSHTPAAASKDPARRIRIVLVDDHAMVRQGLRSILDGYANLIVVG
                     EGADGQDAVVMVRSLRPDVVIMDVNLPLIDGIEATRIVSREYPSIAVIGISVRNDPQV
                     HVAMTEAGAVAFLPKESAASQLYEIILRHCPAIAI"
     gene            25189..>27440
                     /locus_tag="PKK81_01505"
     CDS             25189..>27440
                     /locus_tag="PKK81_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV31571.1"
                     /translation="MTSMLLTLQRVVLSAAGLAIAFPALTLLCWAYDHSILYHLHTSF
                     RTLKPTTMLAVLFCGLSLWLQAEEGAVTTARRYVAQGSAIVACLLASLILSEHLLALD
                     LGIDLWRIALPEISSDAHPGRTAPATAVAILMLGLTLLWLDTKPFATYFFDQSAALIG
                     GTIGCIGLVGYLYGVQSLYKVGTFSSTSVYSALLLVTLGLGILCARPARGLMATLTSE
                     KLGGVMLRRLLPFAVMLPILAGWIRIAAQRSGKYDFNFGVALAVAAVMLIMLAFLWLL
                     AGSLNHTDGQKKHIMQILQQREARHRLALRAGRMGTFHQDLDDESLVFSPELEAIFGL
                     PPMSFASTFAAFQQLIHPDDRQRVQQTVAEAVENRTAYDLECRILRTSPPGESWIAVT
                     GQVLPDSTGHPRQITGVMFDITERKRSETALRQSEEQLRVVTDALPGLIAYVDRDERY
                     RFVNAGYERHFGRSREQIVGSSVKELLQQDYPQVQRFIERVLEGEQISFETTSLGETD
                     EQTLLATYVPERTAGGTVEGFYVLITNITDRKRTEQALRESEERFRHLFEQASDGIVM
                     ADMSGHYLDVNSRACHMLGYSREELLGMGVTDLLDPHDYKRLREIKAELLEGHRHSGE
                     WKLRRRDGATLSVEISAKLQPDRRWHAIVRDITERKRAEAGLRESEAHFRMLADATPV
                     LVWMAGTDTRCTWLNRSWLEYTGRPLEDELGSGWTSGIHPDDQAEYLRVYKAHFSRHE
                     PFEMEYRLRR"
     assembly_gap    27441..27540
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27541..27897
                     /locus_tag="PKK81_01510"
     CDS             <27541..27897
                     /locus_tag="PKK81_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV31572.1"
                     /translation="LVDDHEMVRQGLRSVLDNYEDLEVIGEAGDGESAISIAAALKPD
                     VVVMDVNMPRIDGIEATRRIVSAQPDIVVIGLSVQNEHHVEEAMTRAGAAVFVTKERA
                     AGQLYEAIVRTVRTRK"
     gene            complement(27907..28074)
                     /locus_tag="PKK81_01515"
     CDS             complement(27907..28074)
                     /locus_tag="PKK81_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNV31573.1"
                     /translation="MLSWAVTFLVIGLIAGVLGVSGVAGTATHIAYVLFVVFLILAVI
                     GMVMGKRPPAV"
     gene            complement(28467..29549)
                     /locus_tag="PKK81_01520"
     CDS             complement(28467..29549)
                     /locus_tag="PKK81_01520"
                     /inference="COORDINATES: protein motif:HMM:NF018439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HNV31574.1"
                     /translation="MIPPWKVVLLACVTCAFVAAGAAAAGSDEYIAGYAAATLEHEFH
                     LSGAVIEVDRGAVTVQVKRLGGEDPAKIVRALEQIPGVVRADVQAGKEPVPSSGGTAA
                     RAVTPEAESKFLPRGLLVDPLHADLRWPHFGAAYHNLSSGKEFASAFGESFAFYRNAA
                     PFDGQWELGMQAGVFGVFDTEKRSIDLINADYMVGLLASYRADKLSGFFRVRHQSSHL
                     GDEFLLNNAQVARINLSYEEIDLKVSYDLTTWLRVYGGVGTIVRKDPSTLGRETSQGG
                     VEFKSPWLFFGGKVRPVVYADFQANARTNWNVAQSIMGGLQFENARIGDRKLQVLAEY
                     FSGPSPDGQLFTQKVEWIGMGVHLWF"
     gene            complement(29636..30235)
                     /locus_tag="PKK81_01525"
     CDS             complement(29636..30235)
                     /locus_tag="PKK81_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNV31575.1"
                     /translation="MKTVSPSILMVVLGLTLAVGSMAAPTGAQAADDKGLFEQIDIGL
                     LSIGGRATYVDPKDGSSRWFGGGQVRLHPSRYFAFEGSVDYRRNDIGDTRVHSYPVMV
                     SALIYPLGTTRLAPFLLGGGGWHYTTVKGPGGFDDTQNRFGAHAGGGLQFFFNKHVSI
                     DSTYRYIWLEKIESRDQNIVDKKFQDNGHMVTVGLNFHF"
     gene            complement(30270..30629)
                     /locus_tag="PKK81_01530"
     CDS             complement(30270..30629)
                     /locus_tag="PKK81_01530"
                     /inference="COORDINATES: protein motif:HMM:NF017353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNV31576.1"
                     /translation="MKLRMRLLSMTLTGMVLTSGLSQAGDRSFGSTHGADEHPGTVTV
                     AVNQDQLQGKWKQFKGELKQKWGDFTDDDLLAIEGSVDKLEGKIQERYGDRREDVKRW
                     VDEWFDTHGTQREQTSS"
     gene            complement(30696..30974)
                     /locus_tag="PKK81_01535"
     CDS             complement(30696..30974)
                     /locus_tag="PKK81_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNV31577.1"
                     /translation="MKKIIAMALMSGVLASPAWALFETNKQLASTATVTLEDAVRHAL
                     KAVPGKAVEAEIGKEDGRTVYEVEIVDANNKTQKVYVDAQSGQTKIDH"
     gene            complement(31038..31277)
                     /locus_tag="PKK81_01540"
     CDS             complement(31038..31277)
                     /locus_tag="PKK81_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNV31578.1"
                     /translation="MNTDQFKGKWVQFKGEVKKQWGKLTDDDLMQVEGDYDKFVGRVQ
                     ERYGDKKEEVVKWADDWYAQQGKPRTEGRQAQPQR"
     gene            complement(31747..33888)
                     /gene="glgX"
                     /locus_tag="PKK81_01545"
     CDS             complement(31747..33888)
                     /gene="glgX"
                     /locus_tag="PKK81_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNV31579.1"
                     /translation="MMKVWPGRPYPLGATWDGEGVNFALFSENATSVELCLFDGPDAS
                     KESHRIRLEERTNQTWHAYLPEGRPGLHYGYRVDGPYEPGAGHRFNPSKLLIDPYAKS
                     IADTIRLSDRMFGYRIGDPEGDLARDDRDNADESPKCVVIDQAFSWGGDHLLRTPWSK
                     TVIYEVHVKGFTARHPDVPEHLRGTYAGLSTPPVIEYLQGLGVTAVELLPVHHAVQDK
                     HLTDQGLTNYWGYNTIGFFAPDMRYALSPVRGRHVREFKTMVKTLHSAGIEVILDVVY
                     NHTAEGNHLGPTLSFRGIDNAAYYRLMGDDPRYYMDYTGCGNSLNVRHPRTLQLIMDS
                     LRYWVLDMHVDGFRFDLASTLARELHEVDRLSAFFDIIHQDPVLSQVKLIAEPWDLGA
                     GGYQVGNFPVGWAEWNGKYRDAIRRYWKGDGGQVAELAYRLSGSSDLYEMSGRRPFAS
                     INFITAHDGFSLQDLVSYNEKHNEANGENNQDGHNDNLSWNCGAEGPTEDRDVLELRR
                     RQKRNMLATLLLSQGVPMLCGGDEIGRTQLGNNNAYCQDNEISWHNWDLSRENRTLLT
                     FVRRLIALRQDHPVFRRRRFFQGRRIHGSDITDIVWLHSDGKEMDEADWNQGHLRAIG
                     LVLAGDAIEEKDARGNAIVDDTVVLLFNAHHESIPFVLPACDERTSWVLMLDTCDPTP
                     RRSSAVFKGGEPYTIEARSMAILCRESVHGA"
     gene            complement(33941..>34267)
                     /locus_tag="PKK81_01550"
     CDS             complement(33941..>34267)
                     /locus_tag="PKK81_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNV31580.1"
                     /translation="ESVKSEFLAHAGEEQAHADQLAERIVQLGGEPNLSPEGLADRSH
                     SEYIEGENLEDMIKEDLVAERIAIESYRDMIAFVGSDDPTTRRLLEEILMKEEEHAED
                     LVSLLK"
     assembly_gap    34268..34367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<34368..34558)
                     /locus_tag="PKK81_01555"
     CDS             complement(<34368..34558)
                     /locus_tag="PKK81_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31581.1"
                     /translation="MPFDTTGKQVGLFLSLAACALVVGTQSKCLLQPANDRDPGVEPA
                     AELGLESALPHLRVDLAAP"
     gene            complement(34695..35162)
                     /locus_tag="PKK81_01560"
     CDS             complement(34695..35162)
                     /locus_tag="PKK81_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV31582.1"
                     /translation="MKHEGPRTMEEARARRDRSSLYLESTLDEQLGLLPGEARKVKSA
                     MSSAVTVASPKTSLKEAMSMMTSLNVPVVVVYDGRRLVGMLTDRDIGLNRRVQEAPQD
                     APISGFMRTSVPCCLEDDLLADAISVMHGSQSDWLPVMNRHEQLVGVLSVHAS"
     gene            complement(35192..35488)
                     /locus_tag="PKK81_01565"
     CDS             complement(35192..35488)
                     /locus_tag="PKK81_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31583.1"
                     /translation="MDSHEKVMTIRRWIHPEERITVDFEDERDLNAEVIECESQTVTL
                     LLETAFPHYKQHLTLPLSIVSIGEDKTKYTRNPDKPVEYGRLRLTVHEVRPQAV"
     gene            complement(35593..35796)
                     /locus_tag="PKK81_01570"
     CDS             complement(35593..35796)
                     /locus_tag="PKK81_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31584.1"
                     /translation="MKLLVIRPDPDPQPPDPAPFPGPDPGPFPPQPFPPPIPPLPTRP
                     PIPQLLEQEFMCRDPVPVVSEMA"
     gene            complement(35807..>36471)
                     /locus_tag="PKK81_01575"
     CDS             complement(35807..>36471)
                     /locus_tag="PKK81_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNV31585.1"
                     /translation="CIRDVMEGHMRKIDPYTAKSYDLFGLSGISDHSLAVHFGLYEGY
                     VKAANSLRARLHEILSDGTVDQEEMPAYSELTRRLGFEYNGMILHEYYFGNLRRGGMD
                     RPADGSPFGQAVERTFGSYDRWKTDFCSVGMLRGVGWAICNIDPSTGLVSNHWVSLHE
                     HGNVAGFLPILVMDVWEHAYLLDYQPQERKQYIEAFFSNISWSAVEERLQAACSAPVV
                     PR"
BASE COUNT         7467 a        11213 c        10176 g         7215 t
ORIGIN      
        1 cggatcgatc gaaccggcat tattttggga tacgctcgac ccgtatcatt atgtgcgaat
       61 tcatcggcag gtatcggcgc gggagggaga ggattccgtc ctcatcgtgg gcggcgagga
      121 ccacaaggca ggacaggcgg acgacggaga agcccgctat gctcgcctgg aggcgtgggc
      181 gcgcgagcgt tttcccatga tgaatgccgt cgtatttcga tggtccggac aagtcatgga
      241 gtcggtggac ggcctggcct tcatcggccg gaacccgggc gatacggaca atatttacat
      301 cgcaaccgga gactccggta tgggcatgac ccatggcaca attgccggac tgctgatcac
      361 cgatctcatt atgaaacgcg agtcgccttg gtcctcgttg tatgatccgt cgcgccggcc
      421 ggtgcgcgcc gcaggcgaat ttatccgtga atcactgaac gtcgcggctc aatataccga
      481 ctgggtaacc ggaggggatg ttgaaaccga ggcacagatt ccacatgcac agggagcggt
      541 actgagggat ggactgacaa agatcgcgat ctaccgcgat gcccagggag aactccacaa
      601 gtgttcggcc gtgtgcccgc atctgggctg catcgtggcc tggaaccatg cagaaaatac
      661 gtgggactgt ccctgtcacg gctcaaggtt cgacaaattc ggaaaagttc tcaatggtcc
      721 tgcgacgagc gacctgagcc cactcaagca ggaagatact taggcaacgt catggccgag
      781 agtctgtttt gcaccgacga atcatgcccc gcaccttttc agccttctga tggcgtggat
      841 gggagcaggc caggcgtgga acgtccctag gacacttccc agctttacaa tgggtcggcg
      901 caggttaggg tgtggctcat gccaaacaga tgagtccttc gtgggcgagg agaagaaact
      961 gaatgcgtta cttagcgcta gccgccgact atgacggcac attggcgagt gcaggcactg
     1021 tcgacacgga caccatccaa gccatcgaac gcctggtggc ctcgggtcga aaactcattc
     1081 tggtcaccgg acgggtcttg ccggaaatct tagagatttt tccccagata tctctgtgcg
     1141 aacgtgtcgt ggcggaaaat ggcgcagtcc tctaccggcc ggccactaaa gaaatcacgc
     1201 ttcttgcccc tcctcctcca ccggcattcc tggaagaagt ccaacgtcgc aaggtagagc
     1261 atctcaccct cgggaattcg atcgttgcga cccgggttcc gtatgagacg gtcatcctgg
     1321 acatcattcg tgatctcggt ctcgaactgc gcattatctt caacaagggc gcagtgatgg
     1381 tgctatccca tggcatcaat aaggccaccg ggctgaccgc agcgctcaag cagttggaat
     1441 tgtcccctca taacattgct gcgatcggag acggagaaaa cgatcatgcc atgctgacat
     1501 actccgaata tgccgtggcc gtggagaatg ccgttccgat gttgaaggaa acagcagatc
     1561 gcaccaccgt cggcgatcat ggccacggcg tcatcgagtt gatcaatgag ctggtcgaga
     1621 acgatctggc ggtagcggat cgatcggtag cgcgacatcg gattgcgctc ggcacacagg
     1681 agaacggagg cgacatcacc tttcatccgg cgcgacaaaa cctcttgctt gccggcacgt
     1741 ccggcagcgg aaagtctacg ctggcgacgg ggctactcga gcgccttggc gaacgaggtt
     1801 atcaattgtg tgtgatcgat ccggaggggg actacgagaa ttttccacag gcgattgtcc
     1861 tcggaactgc acaggatggt cccagtcacg ccgaaattct cacggcattg gccaatccga
     1921 acaaccatgt ggtcgtgaac cttgtgggac ttcctctaca ggaccggccc tcattttttc
     1981 tcaccctgtt gcccaaactt caggaactcc ggtcgaaatc gggtcgcccc cattggatgc
     2041 tggtcgatga aacgcatcat ctgttgcccg tcgatgggaa ccccacgaca ccggggctga
     2101 tgaaggattt agccggcatg atttacgtca ccgttcatcc cgaccacatc gaacattcga
     2161 tcctcaagac cgtggatatt gtgttcgccc tgggcaaatc gccggacgag acgttgaagc
     2221 aatattgtgc tgcgatccaa cagccggctc cggccgcgac tgccgcccgg cttcaaccgg
     2281 gtcgagcgat tatgtggaat cgcgcctccg gggagacgcc tttcgtgctt gagatcgcac
     2341 caagcacgat cgaacgccgc cggcatcgac ggaaatacgc ggaaggcgaa ttgcctccgg
     2401 agcaaagttt ttactttcga gggccggccg gacagctcaa tctccgggct cacaatttac
     2461 tgctctttat gcagctcggg gagggcgtcg accaggcgac atggatacat cacctccgtt
     2521 ctcacgatta ttcgacgtgg atcaagcagg ttatcaagga tgaggcgctt gcgcagcgcg
     2581 tacacgacgt ggaacagcag gctcatctgc ccgcagaaga gagccggcaa ctgatccgca
     2641 gcgcgatcga agaacggtac acggtccctg caggcgggga tgaacatacc tcttgacctg
     2701 ggacactatc gcccgcaccg ccaggggatc gcctagtacc gaagttcccg gtcctgcggc
     2761 atcgtaaaag cagccgcgga catgtattcc aggttaatgt ccacgatacg gaaggaaatc
     2821 ggcgacacga gaacagcttc cttccttttt aatccattat cgcaagagga gacgatacca
     2881 tgccgatcct tttatggctt ctgggagtgc cgataagttt gattctggtg ctgtggttgt
     2941 tcggtgtcgt cgggttctga tcacgaaccg ggggacaggc cgtgcaggtc gtcgatgaga
     3001 cgttggatag acgttaccgc cttacgcctc tgttgtnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnctgc aaaatcctgc aaggacggca ttcctgcaac accatgttca
     3181 tcttaccaag gaggaaatcg atgaagaccg cactgcattc gctgctcgca ctggcgcttg
     3241 tttcactggc tgcctgctcc cactcaccga ccgcgtcgaa aacccgctca gagatggagg
     3301 caggcacgaa gaccggtatg gtcaagaaca tcgtcatcaa ggaaacgatc gtccccgatg
     3361 ccttaacggt acgggcgggc gacgaggttc gatggatcaa tcagagacaa gactccgtca
     3421 ccgtgacgat cgatgaaccg ctggaacaca acgtctcctg ccggaacgga ttttccaagg
     3481 cgatgggaat gggcgtagac aactccacga ccttatcgag caatgagacc gctggtctct
     3541 gttttagccg gatcggcacc gtgaagtatt ccgtacgacc ggcggacgaa accaagacca
     3601 gtatggctaa cacccatggc tcgatcaccg ttcagtaatg cgtgaatagc aagccgccgc
     3661 ccacaccatc acggtgtgga cggcggctcc cctcagtaca accagtctca cgtttagatc
     3721 acatttcacg tcatgaggga ctggcgagtc taatttctct ggcccaatgg cgatgcatct
     3781 cgatagcctt tatgaagcgg gctgcgatct gcttgggcga agagtccggc gccagaacga
     3841 ttcctgaatc ctgcgaccag tctttgggcg tctgtctggt tttgcttcca tgttccgcct
     3901 ccgggtaggc cgccccgacc gccgcttcaa acaaggcaac cccctccccc gttgcggcaa
     3961 tcgccttgca atgcaggtag gcgtcctgaa cgaacgttct cgctttgggg gttcgcaaca
     4021 atgcgctgac gctttgttca ccgcctggaa tgtagaccgc atcgaataac accgatcccg
     4081 tcgtcaacag gctgaaatcg atcttcagct cacttccgtt cgcagccttg aggcttcccg
     4141 aacgggtcgc cacaatcttg ccctgccctc cggcggcgac caaggccatc tgcattccct
     4201 tgaccgcctc gccatcgact ccatcggcaa ccagaatcgc cacctttctg gtttgaatgc
     4261 cttccttagg agccaacacc atgctcagcg ccgcagactt gtccagcgaa gtcatcgccc
     4321 gcttgggttg aaagtccttc ggatccccgt cggcggggat actcaaattg agtagaccct
     4381 cgatcttctt gaccggcata cccaagccct ccgcgacccg tccagccagg cctttgtcga
     4441 tctgggacag catgccgacc attcgttccc gaatcgggag gttctcgacc ttgcctagtt
     4501 caaactgcaa cgcctgcacc aaatgctctt gctccggttc agactgactt cgccaaaaca
     4561 gcgcagcctg actgaaatgg tcgaaaaagc tcttgctccg ttctcgcact ttgctgccgg
     4621 gcagccgctc cgcataactc aggaacccgc ccatatctgc cttcgcctgc atgggacaac
     4681 caccccccaa tgtgttcggt tggtaactca ccggcccgga attgatcgtc tgccgcatat
     4741 ggccgtcgcg ttgattgtta tgcaacggcg ccagcggtcg attgatggga atctcatgga
     4801 agttcggtcc gcccagtcga atgagttgag tgtccgtata ggagaagagt cggccctgca
     4861 acaagggatc gttggtgaaa tcgatgccgg gaacgaggtg gccggggtgg aaggccactt
     4921 gttcggtttc ggcaaaaaaa ttatccggat tgcgatcgag tgtcatcttg ccgattcgaa
     4981 ccaccggcac cagttcctcg ggaatgagct tggtcggatc cagaagatcg aattcgaact
     5041 tctgttcgtc cttctcctcc acaatctgca ccccgagctc ccattccggg tactgccccg
     5101 cctcgatcgc ctcccacaaa tcacgccggt gaaaatcggg atccaagccg gaaattttct
     5161 gcgcctcatc ccacaagagt gaatgtacac cgaggagcgg cttccagtga aatttcacga
     5221 accgtccgac gcccttggca ttcacgaagc gaaaggtatg caccccgaag ccttccatca
     5281 tgcggaaact ccgggggatg gcccgatccg acatgaccca catgatcatg tgcatggatt
     5341 ccggcatcag ggagataaag tcccagaacg tgtcatgcgc cgaggccgcc tgcggcatct
     5401 catgatgagg ctccggtttc acggcatgga ccagatccgg gaatttcacc gcatcctgga
     5461 tgaagaagac cgggatgttg ttgcccacca ggtcgtaatt cccttcctcc gtgtaaaact
     5521 tcacggcgaa gccgcgcgca tcccgcgtca gatcactgga acctcgggac ccggccacgg
     5581 tcgaaaaacg cacaaagacc ggggtcttga cctgcgggtc ctgtaaaaat tttgccctcg
     5641 tcagcgatgc gagtgactta tacacttgga aatagccgtg cgcggcggcg cccctggcat
     5701 gcaccaccct ctcgggaata cgctcatggt cgaaatgcgt gattttttcc cgtaggatga
     5761 aatcttccag caatgtcggg ccgcgagccc ccgccttgag gctgttctga tcatcgttga
     5821 tgcgcaggcc ttgatcggtg gtcagaaagt gatcgccgga cttctcccgc gcgctgtcaa
     5881 gggcagcggt tttctcattc tcccgcttgg aacgatcttt gggcatggca cagtcctccc
     5941 ctggtcggcc ttcgccggca tctaccgaat tgttttcctg gatgggcggg cgccggtttt
     6001 tcgaccggat gctttggcca gcaacgtctc cttgcgcttc atgacactgg cctccaacga
     6061 ttcccaatcg atgtcgcact cttcggcttt cggaaacatt tcgccttctt cttcctcgat
     6121 atggtgcttc accagttcac ttaaaacgct gaacttggcc tgaaatttct cgtcgccagg
     6181 tttgagtttc ttcaattcgg cgatgagcac atggacgacg tgatgttcct caatcgcctc
     6241 gttcatcatg tccgcctcat ccacctccgc tcgaattgcc ggataaatca aacgttcttc
     6301 caggtcggca tgaatttcca gctcccggat cgccgttttt gcaatctcgc cccgctcccg
     6361 tccctccgcc gattcgaaat cctcaaacag cttcttgacc ttgtcgtgat cctctttcag
     6421 catttcgaaa accgtcgaaa cgaatcctgc cgctcgtgcc atggtcagct cctttcgaat
     6481 gaagagattc cttactgcca ggcgcaacct atcacgcgga tccaaccggg tgagactcgt
     6541 ggaagcccta gttatcgacg gagtgacgac ccttgtgtac ggactagggc tactcctagt
     6601 ggaccggccc gggggcgtca cgtatgctgc taaagaatcc gttcaacacc ctcaaccctg
     6661 gaggtgtagc catgacaacg ccacggttca ttcccatttg ctgcggttgc aacaatgtgc
     6721 gagacgattc acgacgtgat gagagagaac cgggatgggg aacgttggac gcctatctcc
     6781 acacccatca tctgaccagc ggggactacc ggttgaccca tacctactgc ccttcctgct
     6841 cggttcgttt ttcgaaactg agcggtggcc atgcggtcac gccggaggtt gcggcaagcc
     6901 gttcgctgtg aaacgtgaac gggcagacgt aaaacgtatc caggagcact tcctccgacg
     6961 gattatgacg ccacctgcgc gggaacagcc cgggaaaagt ggcgacgtgt cacgttttgc
     7021 gccccacatt ttacgcctca cggttcacgt ctcaaggaag gtaaatgtcg gccttgaccg
     7081 gcagatggtc cgaggccagt cggctccggg gagtatcgtg cgcttccact gtgagtagcg
     7141 cctgagcggg aaagatccag atccggtcca gcgccaacac cggccatcga gcgggaaagg
     7201 tgaccggagc agccggggaa ccgaacaccc gacgcaaccc gcgcaaggat tttccccatc
     7261 cgtgccactc attcaggtct cccatcaaaa tagtgagatc acgccggctg agtgacagga
     7321 gttccaacaa tcgatccgct tgttgcggcc gttcgcgagg ccacagtccc aggtgggtat
     7381 tgagcacgtg aaccgttcgg ccgcagattg tgagctcgac atccagcgcg cccctcggtt
     7441 cgtgccgacc cactgagaga tcccatcgcc gcacccgatc caccgggtac cggctcagca
     7501 gcgcgttgcc ataccccaca ccttcccgtg accacacagg tccgccgatg gctatcatcc
     7561 cggttttctg cgccagccac ggcgcaatag gagcgtggtc ctgccttgga aaatcaactt
     7621 cctgcaacgc aatgatgtcc gcgttgagtt cttgcgcgac acgcaggatg cgaccgggct
     7681 catatcgccc gtcccgtccg atagcgcgat ggatattgta ggaggctacc cgcagacgca
     7741 tcgtgctcct attcacacgg cctcaggcgg ccggcgccac ctgctgaacg ttgccgacat
     7801 ccggtgcggt gtccctgacc tgtaaccgcc gcgacacgta ccaggcgaca ctccatgccg
     7861 cagccatgag caggcccagc acgccaaagg tgacgggact cggattgcga agggcttccg
     7921 ccaaacggtc gacgaacgcc gacatcagca caattccggg gctcatgccg agcgccgtcc
     7981 ccagcagaaa atcgcggagc gacagcgagg tcgccccggc aaccagattg accatggaaa
     8041 agggcgcgat aggcaggagc ctgacgatca gaatcgccca gaatcctctt tgggcaatcc
     8101 ggcggctcaa atgcgccact cgacggccgg caaaacgctg cactcgatat ctacccaagg
     8161 cccgacccag gccaaaaatg gtcacggcgc ttaggagcgc gccggccagg gcggatggcg
     8221 caccgaccag cgggccgaac gccagaatcg tcaccacaat caacaccgtg ataggaaccg
     8281 cgagaaaacc acccaccagg aaaccgccca gcatgacgag cgaaccggta ggtccctggc
     8341 cgagggcccg cacggcatcg accaggtcgt tcacattcat ccactcactc agagggctcc
     8401 aacgccacgc agccgccaag aggcccagca ggagcagcac cgtatatcca aggaacaccc
     8461 ggcgccccac atgtgcccgc tcccgatgtt gaaccacggc ccccaacacc tcatccgcgg
     8521 ccatgggacg ttcggggtcg atcaggagtt gttccgggat tgaaatggct tcggcggggc
     8581 tcttgtcgaa gcatccatct tccaggcttc gatcgccgcc ccgcaggtga tcgatcgcag
     8641 gccccaggga atccagccgc tggacatgct cagccaccag ctcaggctcg acgccgagat
     8701 gttccccgat caatccattg cgcaatctcg caatcaccgc ctggagccgg gaggttccgc
     8761 cggattcgat cgcgagatta cactccgtat cgaatcccat cgaacgattc gacacattgg
     8821 ccgaccccag gcaaacaaaa tcttcgtcaa cgatgagcaa cttgctgtgc accgccacca
     8881 tgtccctgcc ctgcgtgccc ggcacggtag gcgcgtagat gcgcagacgt ccataatggt
     8941 cggcgagttc cagctcatgc aggatacggg aacggagcgc atccatcgtc tggcgttcca
     9001 accatccgtc gcaattgtga cgaagaacca acacgacttc cggaccatcc tgctccatga
     9061 gccgggccgc cagggcccga ctgatcgcat tggacgtaaa atactgcgac tcgatataaa
     9121 tagagcggcg agcgcgcttg attccttcca gataggccag ttcgatctcc cggacctcag
     9181 gttgccccgc ataggcaggc tgggtgcgca tgataccgac gccgcacggt tccacgtccg
     9241 gcacgagggc gaccggccag agctcctcca ctggtcgttg aatggcaagg ggaagtcgcc
     9301 gtcccgtcgc gttaaaccat cggtcacgag ccaactcacc caaggcagcg gccgcctcac
     9361 ccgacaccat catctgaaca tcgtgaaacg gcgcataggg ttgcccgtcc gcgttgaccc
     9421 gaaggggatc ctggcaagca tgcgcagggg tatcccatcg ggatttcgtc aaatccagcc
     9481 ctccgacaaa agcaatcgtg tcgtcgatga ccacaagctt ctggtgatgc gaggcgccga
     9541 taggatgctg cccatccacc tgataggaga gccgacggtg gccgctccag cccgtctggg
     9601 ccgtcggcat ccattcacgc tccagagcgt agatcatggc gaagtcccaa ttgagcacat
     9661 ggactctcag ctttcgctta cgccgtagga gcgccgacac aaactccccg agtttcgacg
     9721 gaaattcgga cggctctgcc ggacccctga ctaattcgat ctgcgtatcg agatcccatc
     9781 cgacaatcat gatggaatgt ttggcgtgca atgccacatc ccgaaacgtc ttgaaatacg
     9841 cttccccatc gatcaagaat ccgcatcgat cgcttcgttc cacacgccaa caagtgcggc
     9901 ctcgcactaa aaacgacggc ggtgtctgtt cctgcgcctg acatgccttc aaaacttcgg
     9961 ctcgctccat gcaacaccct cccgcatcca cggtcacagt cgttccacgc gcagtcggtc
    10021 tcacaggcga tgcgcgcagt cactttaact cagaccacgc aggatgccca gctggtcata
    10081 cccctagctg ttcgatcggc tccattcgag cggctatcgg gatgtgtcga gaggtttccc
    10141 tagtacctct tgaatcgtgg aacgttagaa tgaacttctc aatctgaact ggaggacggc
    10201 ctatgacgat cgcggcatca aaccattcca tcgcgcagca ccggttcgaa tgtattcaag
    10261 cctgtttgga ttgcgctcgc atgtgcaaca cctgcgggga cgatatgatc ggcatgtctc
    10321 atgaaggggg agaggcaaat ctgatgacac gatgcatcag gctctgccgg gattgttccg
    10381 atatctgcct ccttgcagcc cagtggatgg gccgcacttc ggcctttgct gtccagcttt
    10441 gcgcgttgtg cgccgaaatt tgtgaggtct gtgcgggagt ctgtgagcaa catgcgccgc
    10501 atcatgccct gtgtggagaa tgcgcgacag cctgccgccg ttgcgctgta gaatgccata
    10561 agatggtgat ggaaacagtc ccgagttcct ccggtggtac ggcctagccg ccccgcattt
    10621 gatcgtcagt cgccagtaac cggggaaagg aggtcatcat gttacgcacc atcatcggag
    10681 tcggcctggt gagtggagcc ttgctgacgg ggtggagtcc ttcatggaac gaggcgccgg
    10741 ctccgattcc gcatcccggt cccgggccga ccgttccacc gaaaccgtcc gaccccgttc
    10801 cgcttccacc cgatccgaaa ccggtgctgc cgccccatcc agcaaatact ccgcatgctt
    10861 atctcatggt ggatccggcg acagatccac agaacaccgt gtccggggtg ttggaaaaag
    10921 tggatttgat cgcgaaacag ggactgctca agaccgatct tggtcgggaa gtggcttttc
    10981 aaatcccaaa accggaactc tttctcaatt tgtccgtggg tcaacgtgtc accctcaaac
    11041 tcgatgatcg ccaacaggct gtccgggtga tggacaccac ggcgcctgag attccccccc
    11101 ctttccccgc aaaataaggt acggtgtcat acacgccggt gtcgaactag gggtttcact
    11161 agtggggcga cctggcccaa ggtgctacta cccatgcgca ttgtgcaatg ttgcgtcatg
    11221 ggccccagaa tccgtccatg accggcacat agagtcatca tccagcccga tgcgattgag
    11281 cggcagatga attcaagcga cagcgtcagt tggttgaccg gcagtgagat gggtggccgg
    11341 atccggacgt tcgattggcg ccggaccccg cttgggccga tcgaagattg gcctgccgca
    11401 ctcgtgtcca tcctcggtgt ctgtctgacc gcgcaatatc ccatggccat ctactggggg
    11461 tcggaaggct ggctcctcta caacgatgcc tggcggccta tccttggcga taagcaccct
    11521 tgggcgctcg gtcgcgcggc ccacgaagtg tggcctgagt tgtgggacac catcagtccc
    11581 cttctccata gcgtccagac taccggtcaa gccgtctggc gaggcgacga actcttgccc
    11641 atgcaacggt tcggctatac cgaggaatgt tatttcgact atagcttcaa ccccattcgc
    11701 ggacaaaacg gcgcagtcga gggcatcctg aatatcgtcc aggaaaccac ctatcgggtc
    11761 ttaaacgacc ggcgcatgcg cctcctgcgt gaactggcct cccggtcagg gttcgcccat
    11821 agccaggaag atgcctgcaa tctggccatc gaagcgctcg ctaccgatca agccgacgtt
    11881 ccctttgcgc tcctctacca tatcgaccgg gatcgtcggc acgcacactt aatcgcttcc
    11941 acgggactgc ctccggaaca tccggctcgg caacagaccg tgagcctcac gccggaggag
    12001 cctgattccg ggtggccgct gtcagcccct ctccaagaag gcgttccggt tatcgtcgac
    12061 gacgtaggcg accgcttcgg accactgccc ggcgggtcct ggtcggaacc gaccgggcaa
    12121 gccctcctcc tgccgctcag cacggtacgt tgggatggcc gtgccgtcat cctggcggca
    12181 gggatcaacc cccgccggcc gctggacgac gactaccgct cgttcttcac catggtcgag
    12241 tctcacatcg caggagccct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 tatgtcgatc gtgacatgtg ggagaagatc gttctgaacc tgctcagcaa cgccttcaaa
    12421 ttcactttcc agggccagat cgcgctctca cttcatgcac agacaggcgg ggtggaacta
    12481 cacgtccagg ataccggtgt cggcattccg cccgaagccc tcccgaggct cttcgaacgt
    12541 ttctatcgcg tgcaggatat gcgcagccgg acttatgaag ggagcggtat cggcttggcg
    12601 ctggttcagg agttggtcca acttcacgga ggaaccgtcc gggtcaacag ccaggtggac
    12661 caaggcagca cctttgtcgt tacgctgccg ttcggaaacg cccatctccc gccggataag
    12721 gtcgtcccgc acggccgacc aaccacaccc acgacgggag tcgggcccta tctggaggaa
    12781 gcactcctct ggctgtctga accggagcgc gagtcgaagc cggcggacgg gaggcctgat
    12841 cctgccgaag cccctctctc cacaacgagc gcggagacgg cccgacccag ggttctcgtg
    12901 gtcgatgaca atgcggatat gcggcaatac atcgcccgtc tcttggcgga tcggtatcac
    12961 gtcattgcca tgccggaagg catgtccgcc ctgatcgagg cccgccggca accgccggat
    13021 ttgattctca gcgatatcat gatgccgcaa ctggacggtt tcggactgct tcaagccctg
    13081 cgcgacgacc ccgccctcaa gaccattcct gtcattttgc tctccgcgcg cgcaggcgaa
    13141 gagtccagaa tcgaagggct ggggcgtggc gcggacgact atctcgtcaa acctttcggc
    13201 gcccgtgaat tactcgcgcg agtcgccacc catctcgaca tggcgcgcgt ccgacgtgag
    13261 gcgcaaaggg agattgcaca cagcgaggca aggtttgagg ccatcgtcag ccaagcgacg
    13321 gccgccgtcg cgcagaccga tctcaccggg cgattcctgt tcgtgaatca acgcttctgc
    13381 gagttgctcg gatacagtgc atccgaactg ctcgggatga atatgcagca ggtcaccgat
    13441 ccggatgacc tgccggccaa cctcgtgctc tttcatcggt tgaccgccgg cggtccggac
    13501 ttcgtgattg agaagcgata ccggcgaaag gacggctccg cggtctggac cagagtcagt
    13561 gtgggcgggg ttcgggaaga cggcgaactg cgccatatcc tggccgtggg gctcgacatc
    13621 accgaccgca aacgtcagga agcggaactc ctggccctca cccaccgaca atccctcttg
    13681 tacgacctgg ccgatgtcgt gaaccgggcg gaggccttga ccggcctcta cgagaaagcc
    13741 atcgacacga tcatcgaggc cctgcatgcg gaccgcgcct cgattcttct gttcgacgac
    13801 gagggcgtga tgcgcttcga agcctggcgc ggcctgtcgc aagcctatcg agaggcagtc
    13861 gagggccatt ccccctggat tcccgaccag caggatcccc cggccatctt cattcccgat
    13921 gtcgccgcat ccgacctcca cacgtctttg cgatccgtta ttcagagcga aggtgtctcc
    13981 gcgctgggat ttatcccgct gacgtatggc ggccggttac tcggcaagtt catgatgtat
    14041 ttcgaccggc cccacgtgat ggacgagcag gagataggct tggcccagac gatagccagg
    14101 acactggccg tcgggatcga acgggctcgg gccggcgagt tattgcggtc caatgaggaa
    14161 cgacttcgtt tggccatggc agccggctcg atgggtgcgt gggacgcgga cctctcgacc
    14221 ggagcaaccg attgggacgc caaacaatac gagttgttcg atagaccgaa agacaagccc
    14281 ctcacccatg cggagcagtt ctacgaatcc atccaccccg acgatgtgcc ccgcgtgagg
    14341 gaagcggccg cgcaagccga gctgacgggt tcattctctc aagaatttcg agtgatcgcg
    14401 caggacggct cgatccgctg gctctccggg cacgggacgg cggtgaagaa cgaccaggga
    14461 caggccgtcc ggatggtcgg gatcaattac gacattacgc gacgaaagca cgtggaggaa
    14521 gccctccagc ggtccgcctc cgacctggag cagcgggtcg tggaacgcac cctcgaactg
    14581 tctcagtccc gggaacagct gcgggcgctc gccaccgaat tgaatctcgc cggacagcgg
    14641 gaacggcaac atctggcaac tgaactgcac gattatctgg cgcaacttct ggccctgagc
    14701 cgaatccgtc tggcccaggc caaacagcaa cccatgacga cggccctggg aaagatcctg
    14761 agcgagttgc aggacgtgat ggatcaggcc ctgacctata cccgcaccct tgtggcccaa
    14821 ctcagcccgc cgattctgaa agaattcgga ttgccgatgg ccctaaagtg gttggcggaa
    14881 caaatgatgc aacgggacct gcaggtatcc ctggagctcg gaacagatca tctcgccctg
    14941 cctgaggacc aggccatgtt gctgttccaa tccgtacgcg aactgctcat gaacatcgtg
    15001 aagcatgcct ctgccaaaca ggcccggatc acggtcaggg aagaggaggg atggctgcat
    15061 atcaccgtcg cagaccaggg ccgaggcttc tcccctcccg attccattcc tgcgacgaga
    15121 tcctcgggca tctccggctt tggacttttc agcatacggg agcgtatgct cgcgcttggc
    15181 ggccgttttg aattgcaatc ccatcccggt caaggcaccg aagccacatt gaccgcaccg
    15241 cttgcaccga gacagcctcg cgcgaaacca caggcaggag ccgacgcgag cgtaccgggc
    15301 gctctgtctc acaagacggc acacgcggcg actgtgcggg aaggcctcgc cgacgaacgt
    15361 gaacctgtca atcgccccat ccgcgtgttg atcgccgacg accatgccat ggtgcgacag
    15421 ggcttgtgcg gacttctggc cgcctatggg aatatccagg tcgtgggaga ggcagccgat
    15481 ggtgaagagg ccgtcaccct gacgatacag ctacagcccg acgtcgtcct catggacgtc
    15541 aacatgccgc aaatggacgg aatcgaagcc accaggcgga tcaagcaagt ccttccggcg
    15601 acgatcgtca tcgggctctc cgtccaaaac gctgcccatg tgggacgcgt gatgagagaa
    15661 gcaggggctg cggcctttct caataaagag gccgccgtgg aagacttgta ccagaccatc
    15721 caggtcgccc ggaaggaact gctgcaggtc ccatgagacg attccaacaa tcccccaatc
    15781 ccctgctgtc cggagcaatc gtcttctctc tggcaaccgt cttcgtgctg gatttttaca
    15841 cgccgctcgg catcaccgtg tgggtgctct acctgatccc ggtcgtgctc gcctatctct
    15901 tctggtttcc tgtcacgccg gtggccgtgg caggagcggt gacaatcctc actctagcgg
    15961 gcctcttgct cagcgcccct ggcatcgacc cctccgtttc acgcatcaat cgcctggttg
    16021 gaatcattac ctcctgggcg ctggcggcct tcggcgcgca tttcatccgc aaccggatcg
    16081 ccgtgcgcaa agaagaatgg atacgggcag gccagacgct cctcagcgaa cggatgatgg
    16141 gagagttggc gctgaacagc ctgggcgaaa acctgttgca ttgcctcgca aaatacctgg
    16201 gagctgaggc cggcgccatt ttcatcgacg acgggcgcgg ctttcaccgc acggcgacct
    16261 atggcgtccc ggaaggcact gccatgccgg aacggttcgc agcgggcgac ggactcctgg
    16321 gccaggcgct acagcacaaa cacacagtcg tggtgcatga cttgccggaa gggtatttca
    16381 ccatcggctc cgctttggga cggagtgccc cacgccacct gatgatcgcg ccgttgaccg
    16441 tcgatggacg cgtcaacagc gttttggaac tcggattctt tactcgcgtg acggaatccg
    16501 accgggagtt tgtcgatcgc gtggcggaat cgatcgccgt cgccgtccgg tcggcccgat
    16561 accggactcg ccttcaggat ctcctggagg agacccagcg gcaagccgaa gagttgcaag
    16621 tgcaaagtga ggaactccgc gtcgccaacg aagaactcga ggaacagagc cgggcgctga
    16681 aggaatcgca cagccgcctc gaacagcaac aggcggaact ggaacagacc aactcccaat
    16741 tggaagagca ggctcaaatc cttgaaattc agaaggaaga tctcaatcaa tcgacggcga
    16801 ttctcgaatc gcaaaccaga cagttagagc gggccagtcg ctataagtcc gagtttttgg
    16861 ccaacatgtc gcacgaaatg cggacccccc tgaactcgac gctgatcctg acgaaactgt
    16921 tggccgacaa tccacagggc aacctcaccg ccgaccagat caagtccctc acaacgatcg
    16981 aatcctccgg ccacgacctg ctggcgctga tcgacgacgt cctggacctg gccaaggtgg
    17041 aggccggacg tatcgacctc accccgcaac cagtgccgat cccgcggctg ctcgacagct
    17101 tgagatcgat attcaaacca ctcgcggaac aacgggggct ccggctcacc atgcaacagc
    17161 ggccgggcac gcccgactcg ttcgaaaccg actcgcaacg gctcgaacag gtgttgaaga
    17221 atctcctgtc gaacgccatc aaattcaccg aggtcggcga agtgtcgctg gaagtatccc
    17281 gcctctcgga cgggcgtctg gcgttcacgg ttcaagacag cggcatcggc attccgcaag
    17341 accagcatga gttgattttc gaacccttct gccaggccga cggcaccacc agtcgaaaat
    17401 acgggggaac cggactcgga ctctccattt cgcgcgaatt cgttcgattg ctggggggag
    17461 acattcatct gacgagtacc ccgggccagg gcagcagctt caccgtgctc ctcccccaac
    17521 gcttatcgtc cgcgccattt cctgcgcctg cccgttccct ccatccacaa tcgccgccca
    17581 aaccgatgag gcccggcgac attcccccca tccctgcaat cccgtctgag agcctggtgg
    17641 cgacgacaca catcgaggat gatcgggaac agctggccgc caacaggcgg gtgattttga
    17701 tcgtcgagga tgacgaaccc ttttcgaaga ttcttgcgga cctggcccat gaactgaatt
    17761 ttcaggtcct ggtcgccacg acggccgccg atgccctggc gctggcctcg cgctacctgc
    17821 cgagcgcgat catcctcgat atcggacttc ccgaccactc cgggctctcc gtcatcgatc
    17881 gcctgaaagc cgatccccgg acgcgacata ttccgattca cgtcgtctcg ggtcacgatt
    17941 atgagaggac cgcgctctcg ttaggcgccg tcggctacat gctcaaaccg gtgaaacggg
    18001 agcagctggt ggaggccttc cggcaattcg agaccaggct gacggataaa ccgcggcgag
    18061 tgctgatcgt ggaagacgac cccgcccagc gggagagcct gcgtctactg ctcggttcgg
    18121 acgaagtcga aacgatcggc gcggacagcg cggcggaatg tctcgcccat ctcgccgagc
    18181 gaaccttcga ttgcatggtg ctcgacctca cgctgcccga tgcgtcgggc ttttcgttgc
    18241 tcgaaaccct cagccgggag gagcacctcg cgtttccacc ggtgatcgtc tatacgggcc
    18301 gggagctttc tcccgatgag gagcagcggc tgcgcaaata ttcgagttcg atcatcatca
    18361 agggcgcgaa atctccggaa cggttgttgg acgaagtgac cctgtttctg caccaggtcg
    18421 tgacggcgct tccaccggag caacagcgga tgttggagaa ggcgcgcagc cggaacgccg
    18481 tgctggaaag ccggcgcatt ctcatcgtcg aagacgacgt acggaacatt tttgccttga
    18541 gcgcggtcct ggaaccgcat ggggcgaaga tcgagatcgc gcggaacggc cgcgagtcgc
    18601 tgcgcatcct ggaggaatcg cttcaacacg acgaccggct gatcgatctg gtgctgatgg
    18661 atatcatgat gcctgaaatg gacggcctca cggccatgcg cgccatccgc gaacggcccg
    18721 aatgggacac acttcccatc atcgccctga cggccaaggc gatgaaaaac gaccaggagc
    18781 aggcgctggc cgccggggcg aacgactaca tggccaaacc gctcgatgtc gatcaattgc
    18841 tgtcgctcgt gcgcgtatgg atgccccgat gacctccgac acggcgatga agacagaaga
    18901 tatcgaactg cagctgttcc ttgaggccgt gtatcaacgg tatcactatg attttcgagg
    18961 ctactccaag gcctcgatca aacggcggct cctgctggca cgcgaacgca tcgggtgcac
    19021 caccttttcc gggctacaag ataggatgct gcacgatcat gcggtgcttc ctcagttgct
    19081 ggactacctg accgtgcagg tcagcgatat gttccgcgat cccggctatt ttcgcgcgct
    19141 acgggaacag atccttcccc acctcaagac ctatccctcg ttgaaggtct gggtggccgg
    19201 ctgcagttcg ggtgaagaat tgtactccct cgccattctg ttccgtgagg aagggctgga
    19261 gagtaagacc atgttttatg ccaccgacat caatgccggc gcgctcgcca aggcagaggc
    19321 cggggtctac gatctccagc ggatcgcgct ctttacgagg aaccaccgtc tggccggtgg
    19381 gagaggttcg ctatcggact actacacggc cgcgtacggg gcggccaggt tcgataaaac
    19441 gctccggcgg cggacggtct tttccgacca tagtctggtc tcggattcgg tctttgccga
    19501 ggtgcatctg gtgtcctgcc ggaacgtgct gatttatttc gaccggccga tgcaagaccg
    19561 cgcgatcggc ctgttcaagg aggccctcgt ccgaaaagga tttctcggta tcggcgccag
    19621 agagagcctc cgtttcagcg cccacgtcaa ggcgttcacg gagttacttc ccggcgagcg
    19681 gatttatcag aaacgaggcg atgagtgatg gctctccccc tgcatggatg cctgcgagaa
    19741 ccacgcgcca tcgtcatcgg cgcgtccgcc ggcgcggtca ccgcgctttc gcgcctcctg
    19801 cccccgctgg cggcagactt tccgctggca attctggtga cggtacacgt gccggccgac
    19861 aagcccaaca gtattccggc cttgttgcgg accaagtgcc ggattgcgat caaggaagca
    19921 gaggataagg agcccatcgt gcccggaacc gtctacttcg ctccgcccga ctatcatctt
    19981 ctcgtggagc aggatcgacg cctgtcgctg tccaatgagg aaccggtgaa cttctcccgc
    20041 ccgtcggtcg atgtgctctt cgaatcggct gcggacgcgt acggcaagga cttactggcg
    20101 atcattctca gcggcgcgaa cgaggacggc gccagggggg ctcgcgcagt ccgcgaagcc
    20161 ggcgggatcg tcgtggtgca aagcccggat tctgccgaag cccgcatgat gccggaatcc
    20221 gccttgacgg cctgtccgaa ggcgcgagcg atgaacctgc atacactcgc ggagctgttg
    20281 tgcgcctgtg gaggcaccgt ccagtgctga ccgattcacc ggccaaattc cttttagtcg
    20341 acgaccatga gcccaacctc gtggccctga gcgccgtcct gtcccgtgac gacgtcgaaa
    20401 tgttacgcgc caggaccggg agggaagcgc ttgagctgtt gctccaacac gacatcgccc
    20461 tggctgtcat cgatgtccag atgccgatca tggacggctt tgaactggcg gaactcatgc
    20521 gtggctctca gcgcacccag catgtgccga ttatttttct gacggcaggg cctcaagaca
    20581 acctgcgccg ctttcgcggc taccaggccg gtgcggtgga ttttctctat aagcccattg
    20641 agccggacgt gttgcgcagc aagaccggga ttttcctgga cctgtaccgg cagcgggagg
    20701 agctggcccg gcagcgtgac acattcatca cgctcgctga agaaaaggcc cgcctcctgc
    20761 gggaacgcga cgaggccaat cgtcggctgc gcgaaagcga aatgcgcttc aggacattgg
    20821 ccgacagcgc gccggtgatc atctggatga acggaccagc cggctgcgaa ttcgtcaacc
    20881 agacctgcct cgatttcctg ggcatcgacc acctgggcca gatcgacata ttcgactggg
    20941 ctcgccacgt ccatccggac gatcgcgacc aggcggccgc ggtctaccgg caggcggtcg
    21001 agggccgaac ccgtttcgag gcctctttgc gctgtcgtcg acgggacggc acctatcgat
    21061 ggattcgttc tgtcggcatg ccgaatctct ctgccgacgg aggcgtcctg ggttatatcg
    21121 gggcgagttt cgacgtcacc gaaagcaagg aagccgaaga acgcctgcaa cgatggagcg
    21181 tggaccttga acgggccgtc agccaaaaaa catcggagtt gctgcaatcg caggagcagc
    21241 ttcgtgcgtt ggcgacggag ttgaacctga ccgagcaacg ggaacgcaaa cgactggcca
    21301 cggagctcca cgactacctg gcccaactgc ttgtggtcgt gcggatgaaa ttacggcaga
    21361 ccattcctct ggtcacgggg gaacggatcc ccgaactgtt gaaagacgcc gaccacgccc
    21421 tgactcaatc cctggactac acccggtcgc tcgtcgcaga gttgacgccc cccaccctca
    21481 aggaattcgg cctgctccag gcgctgacct ggctggccac acagatgcat cgccacgggc
    21541 tcaccgtcac cgtgcgacag gacgcagcgc ctctgccgct gccggaagac caggcggtgc
    21601 tcctgtttca atccgtgcgc gaactgcttt ttaacgtgct caaacatgcc aaggctacag
    21661 aggcgaccat cgtgctcacg gtgagagccg acggccagtt ggaggtggtg gtcgccgatg
    21721 acggctgcgg gtttgtcact gcgccgaaga atcgacccgg aaccaccgcc aaccgcttcg
    21781 ggttgttcag cattcaagag cgcatggccg cgatgggtgg gcaattggtc atcgaatccg
    21841 tcctgggtgg cgggacacgc gccacactca cgacgcccta ttggaacagc cgggagaaac
    21901 cggaggacga cgacaccctg accccggcgg aatcacgggc accctcgctg cggacccggt
    21961 ccacagacgt ggagcacctt tcccattctc atcatcccac gtcgaaaatc ggcattctgc
    22021 tggtagacga ccacgccatg gtccgccagg gattgcggag cgtgctggaa cattatgacg
    22081 acgtcgcggt ggtgggcgaa gcttcaaccg gccgcgaagc catcgagttc gtggaacggc
    22141 tgcggcctgc ggtgatcgtc atggatatca atatgccgga gaccaacggg atcgacgcga
    22201 cggtcgaaat caaggcacgg catccggacg tggccgtcat cggactctct gtccacaaca
    22261 ctgccgaggc acggaaggcc atgctgcagg ccggcgccac caccttgctc tcgaaagagt
    22321 cggccgtcga cgaactctac gccgcgatcc gacaggcgct ggcagacgag ggagtgccac
    22381 ggtgagtcgc cggtcaccac atgacgatgg agcgccagcg atgccccaaa tccgcggtcg
    22441 ggacggacat tcgtacgatg cctgcgtcga tggtcaccct ggcatgttcg atttgggcat
    22501 ccgtcgggat cggcacgtat cgttgatgcg acccgctgct gagaatgatg ccctgccggg
    22561 cgaactcaac ggcaatctcc tcggcactgg ccatctccgg catcgctcga aagtctgcat
    22621 agacattgaa cgtgccatcg gactcggaca cctcaatcaa ggggccttgc tgaacagcgc
    22681 gagaggaacc gagtgaaccg gcagttgtct gtgcggatgc ttgccgggag tcattcaatc
    22741 gcattccatc gatctcgcca cccaatgtct gcttcatact tgctccaatc gttatgccat
    22801 catgttgaag gagtctcgct gtgtcctgcc ggtaccttac ggcatactgt cgggcaaacc
    22861 actaggattt tcccgcggtc cagacctgga agacgctagc ggtttgccta gttgttcacc
    22921 cgggcccgac atcctaatat cacgtccgcg cccggatcgg tctttgttcg cgcacaatga
    22981 ggcacactga gatgcattcg acagacggag acaaccccac tgcttccgag attccgcttc
    23041 agattgatct cctcctgtcg cgtctggctg cgatcgtgga gtcgtccgat gacgccatca
    23101 tctccaaaga cctcaatggc tgcatcatga gttggaatca gggggcgcaa cgcctcttcg
    23161 gatacacagc acaggaaatg atcggacagc cggtgcatcg cctgatcccc caagaccgac
    23221 tcgatgaaga acccagaatt ctggaacgcc tccgcctggg gcaacggatc gaccattacg
    23281 aaacggtgcg gcagcgcaaa gatggaacga tgatcgacgt ttcgctttcg gtgtcgccga
    23341 tcaaggatcg gggaggccga attgtcggcg cctcaaaagt cgcccgggag atttcggaac
    23401 gaaaacaggg tgagcgtgac ctggcaaatc tggtcgaagc ccttccggtc gcggtctaca
    23461 cgaccgatgc cgagggacga atcacgcgct acaatcaggc ggctgtggat ctctggggat
    23521 ccagacccgt gctcggcacc accagatggt gcggctcatg gcgggtctac tggccagacg
    23581 gtcgccccat gccgcttgca gcctgtccgc tcgcccagtg cctcaggctc aatcgcccga
    23641 tacgcggact tgaaacgatc attgaacgcc cggacggaac ccgggtcccg gtcgcacccc
    23701 accccacccc ccttcgcaac gcggccggag aattggtcgg cggggtggac gtgctcatgg
    23761 acctcacgga acggaaacgc gcggaacgcc aactgcagga gtccgcggct gagctcgaac
    23821 gtcgcatcaa ggaacgcacg catgaactcg tcctgtctca ggaacggctg cgggcactgg
    23881 cctcggaact gacgttgact gaacaacgcg agcgccgacg gctcgccgtt gaattgcacg
    23941 actatcttgc gcagctcgtc gtggccagcc ggctcaggct ttcgcaagcc atccccaagg
    24001 ttcaggatga aaccgcatcc gccgcgctga cgaacgtgga cgggatgctg gatcaggcct
    24061 tgacctacac ccgatcgctc gtggccgaat tgagccctca catcctctac caattcggcc
    24121 tctccaaatc gctggtctgg ctgggagagc ggatgaagca acacgcgctt gaagtcacgg
    24181 tcaaaacggg tccgacgtcg tttaccctgc ccgacgacca ggccgttctc ttgtttcaat
    24241 cggtccgcga attgctgttt aacatcgtca aacatgccaa gacagatcgc gctgtcatca
    24301 cgatcaacgt cgatgagcaa cgggagctgt ggatctgcgt cgaggatgaa ggcgtcggat
    24361 tcgacgtgac ggaactgacc gacggcgagg acagccgggg aaaattcggc ctgctcagca
    24421 tccgggaacg gatggaaacg ctgggaggag aatgcgaact ctccgccgcg cccggcgccg
    24481 gcaccgtcgc gatcctccgc ctgccgctct ccgggagcgc caccgcctcg ccttccacgc
    24541 cacccaccac ggttccttcg catacgccgg ccgccgcgtc caaagaccct gcccgacgga
    24601 tcagaatcgt gcttgtcgac gaccacgcca tggtccggca aggactgcgc agcatactgg
    24661 atggctatgc gaacctgatc gttgtcggcg agggagccga cggccaggac gccgtcgtga
    24721 tggtccgttc gcttcgcccg gatgtcgtga ttatggacgt gaacctgccg ctcatcgacg
    24781 gcatcgaagc cacccgcatc gtgtcgcgcg aatatccctc tatcgccgtc atcggcatct
    24841 cggtgaggaa cgacccccag gtgcatgtcg ccatgactga agccggagcg gtggcattcc
    24901 tcccgaaaga atccgcagcc agccaactct acgaaattat cctccgccat tgccctgcga
    24961 tcgctatatg agatttcgcc cctgacggag gaggactagg gtatccccca gttctcaatc
    25021 gtcttccgac gcggtatgct gtttgtaccc gtgtgtaccg ccgcttgcct tgctgttccg
    25081 acctgaccga ctctcgtgcc tgcactgggt atgtccttcg atgaagagcc tgcgaaaggc
    25141 cttcatcgca cgatgtgacg gcatcaaccg aaggtatgtt gttctcgcat gacctcgatg
    25201 ctcctcacat tgcagcgtgt ggtcctatcg gccgccggac tggctatcgc gttcccagcc
    25261 ctcacattgc tctgctgggc ctacgaccat tcgatcctgt accatctgca tacgtctttc
    25321 cgcacactga aacccacgac catgttggcg gtcctcttct gcggcctatc cctctggttg
    25381 caagctgaag agggcgccgt cacgaccgca cggcggtatg ttgcacaggg ctccgcgatc
    25441 gtcgcctgcc tgcttgccag cctgatcctg tcggaacacc tccttgctct cgacctggga
    25501 atcgacctct ggcgcattgc cctcccggaa atctccagcg atgcacatcc tgggcgcacg
    25561 gctcctgcca cagccgtcgc cattctgatg ctgggactga cactgttgtg gcttgatacc
    25621 aaaccgttcg cgacatactt cttcgatcag tctgccgcac tgataggagg aacgatcggc
    25681 tgcatcgggc tggtcgggta tttgtacggg gttcagagcc tttacaaagt ggggaccttc
    25741 tcatccacct ccgtctatag cgccctcctg ttggtgacat tgggcctggg aattctctgc
    25801 gcgcgaccgg cgaggggcct gatggccacc ctcaccagcg agaagctcgg gggggtgatg
    25861 ttgcgacgtc tcctgccctt cgccgtcatg ctgccgatac tcgccggatg gattcgcatt
    25921 gcagcccagc ggtcggggaa gtacgacttc aatttcggcg tggcactggc tgtggccgct
    25981 gtgatgctga tcatgctggc gttcctctgg cttctggcag gaagcctgaa ccacaccgac
    26041 ggccagaaga aacacatcat gcaaattctc caacagcgtg aagcccggca tcgcctggcg
    26101 ttgagagccg gccgcatggg cacatttcac caggacctcg acgacgagtc gctggtgttc
    26161 tcccctgaac ttgaggccat tttcggcctc cctcccatga gcttcgccag caccttcgcg
    26221 gcctttcaac aactgattca tcccgatgat cgacaacggg tgcagcagac cgttgccgag
    26281 gcggttgaaa acaggaccgc ctacgacctt gagtgccgga tccttcgcac gagccctccc
    26341 ggtgagagtt ggattgcggt gaccgggcag gtgctccccg actcgaccgg gcatccgcga
    26401 caaatcaccg gcgtcatgtt cgacattacc gaacggaaac gatcggaaac cgcgcttcga
    26461 cagagcgaag aacaactgcg ggtcgtcacc gatgcgttgc ccggcctcat cgcctatgtg
    26521 gaccgcgatg aacgataccg ttttgtgaac gccggatatg aacggcattt tggacggtct
    26581 cgtgagcaga tcgtcggaag ctctgtcaaa gaactcctgc aacaggatta tccgcaagta
    26641 caacgtttca tcgagcgggt actggagggc gagcagatct cattcgaaac taccagtctt
    26701 ggtgagaccg acgaacagac gctcctggcg acctatgtcc cggaacggac agccggggga
    26761 acagtcgaag gcttctatgt cctcatcacc aacatcacag accggaaacg gacagagcag
    26821 gccctgaggg aaagcgaaga acggtttcga catctttttg agcaggcttc cgacggtatc
    26881 gtcatggcgg atatgtcggg ccattacctg gatgtgaata gtcgggcttg tcacatgctg
    26941 ggatattctc gcgaagaact gctcggcatg ggcgtgaccg acctgctcga tccgcatgac
    27001 tacaaacgcc tgcgggagat caaggccgag ttgctggaag gacaccgtca ttccggtgaa
    27061 tggaagttgc gcaggaggga cggagcgaca ctgtccgtgg agatcagtgc caaactccag
    27121 cccgacagac gctggcatgc catcgtccgc gacatcaccg aacgcaaacg ggccgaggcc
    27181 gggctgcggg agagcgaagc ccatttccgc atgttggcgg atgccactcc ggtgctcgtt
    27241 tggatggcgg gcaccgacac ccgctgcacc tggctgaatc gatcgtggct tgagtacaca
    27301 ggccgtcccc tggaagacga gctcggcagc ggatggacat ccggcattca tccggacgat
    27361 caggccgagt acctgcgagt ctacaaagcg cacttctcgc ggcacgagcc gtttgaaatg
    27421 gaataccgcc tccgccgtca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27541 ctcgtcgatg accacgaaat ggttcgacaa ggcctacgga gcgtgctgga caactacgag
    27601 gacctggagg ttatcggcga agcgggtgac ggcgagtcgg cgatcagtat cgccgcggcg
    27661 ctcaagccgg atgtcgtggt gatggatgtg aatatgccga ggatcgacgg gattgaagcg
    27721 acccgccgca tcgtctcagc gcaacccgac atcgtcgtga tcggattgtc ggttcagaac
    27781 gaacaccatg tagaagaggc catgaccaga gccggggcgg cggtctttgt gacaaaagaa
    27841 cgcgccgccg gccagctcta tgaagcaatc gtgcgcacag ttcgcacgag aaagtaggag
    27901 cggcctttag acggcgggag ggcgcttgcc catcaccatg ccgatcaccg ccagaatcag
    27961 aaatacgaca aacagcacat aggcgatatg ggtcgcggtc cctgccacac cgctcactcc
    28021 caacaccccc gcgatcagcc cgatgactaa aaatgtcaca gcccagctca acatgatggc
    28081 acctcatctg aaatggtgga cgatacgctt gcaccgcatg gaacctctat gaatgcaatg
    28141 gatgcttcgt ggcgcagccc ttgtgcccgc aggcacaggc tccgctggga gccgacgaac
    28201 tgcgacaggc gtcggaacaa aactcccgcc ccttctcagc agggcaggaa catccgggat
    28261 gcgcacagga tttctgtcca ctggcgggat ctggcatcat gacctccttc cagacgaccg
    28321 aggccgtctt tcatgacgga gttgcggaac cgaacgaaca cccataagca ccacgggtcc
    28381 caccgagaag gcgagcggca cagctcgata cgtttctacc gccggtccgc tcatcagaaa
    28441 aacgggccgc tcatcgcagg cttggatcag aaccagagat ggactcccat tccgatccat
    28501 tcgacttttt gtgtgaacag ttgtccgtcc ggactggggc cggaaaagta ttcggctaag
    28561 acctggagct ttctatcgcc gatccgggca ttctcaaact gcaaccctcc catgatcgat
    28621 tgggcgacat tccaattcgt gcgagcgttg gcttgaaaat ccgcatagac gaccggccgc
    28681 accttgccgc cgaagaacaa ccagggactc ttgaactcga ctcctccctg gctcgtttcc
    28741 cggccgaggg tgctgggatc ctttctgacg atggtaccga ccccaccgta gactctcagc
    28801 catgtcgtca ggtcatacga caccttgaga tcgatctctt cataactcag attgattctg
    28861 gcgacctggg cattgttgag gagaaactca tcgcctaaat gcgaactctg gtgacgaacc
    28921 cggaagaacc cggagagttt atccgcgcga taactggcca ataacccgac catatagtcg
    28981 gcattgatca aatcgatgga ccgtttctcc gtatcgaaga cgccgaacac ccccgcctgc
    29041 atccccaatt cccattgtcc gtcgaacgga gcggcgttcc ggtagaaggc aaacgattca
    29101 ccgaacgcgc tggcaaattc cttcccggat gaaagattgt gataggccgc gccgaagtgt
    29161 ggccacctca ggtccgcgtg caggggatcc acgagcaatc cccgcggcag gaactttgat
    29221 tctgcttccg gcgtcaccgc ccgcgctgcg gtgccccccg acgacggcac cggctctttc
    29281 cccgcctgaa catcggccct caccacgccg ggtatctgct ctaacgctct gacgatcttg
    29341 gcagggtctt ctcctcccaa tcgtttgacc tgtacggtga cggcgccacg gtctacttct
    29401 atcaccgctc cagaaaggtg aaactcgtgc tccagggtag cggccgcata gcccgcgata
    29461 tactcatcgg agccggcggc cgccgccccc gccgcaacga acgcgcaggt cacacacgcg
    29521 aggagcacca ccttccatgg agggatcatt cccttcggac tcctctcaga gtcatgcccg
    29581 atccagaccg tctccgcctg ttatccagaa tcacagatcg aaccatagcc acaccctaga
    29641 aatggaagtt gaggccgacc gtgaccatgt ggccattgtc ctggaacttc ttgtccacga
    29701 tgttctggtc tcgagactca atcttttcca gccagatgta gcgataggtg ctatcgatcg
    29761 agacgtgctt attgaagaag aattgcagac cgccaccggc atgcgcgccg aaccggttct
    29821 gcgtgtcgtc gaacccgccc ggtcctttca ccgtcgtgta gtgccagccg ccaccgccca
    29881 acagaaacgg agccagcctt gtggtaccca ggggatagat caacgccgac accatcaccg
    29941 gatagctgtg cacccgtgtg tcgccgatat cattcctgcg ataatccacc gaaccttcaa
    30001 atgcgaagta ccgggacggg tggagccgca cctgcccgcc tccgaaccag cggctcgacc
    30061 cgtctttagg atcgacatag gtggcgcgac caccgatgga gagcagcccg atatcgattt
    30121 gctcaaacag ccctttatcg tccgcagcct gcgcacccgt gggggcggcc atgctgccga
    30181 ctgcgagtgt caaccccaat acaaccataa gtatcgaagg ggaaactgtt ttcattgttc
    30241 actcctctct gaatgtgtcg tgtagggcat catgaggacg tttgctctcg ttgagtgccg
    30301 tgggtgtcga accattcatc gacccagcgc ttgacatcct cgcgccggtc gccgtatcgc
    30361 tcctggatct taccttccag tttatcgacg cttccttcga tcgccaggag atcgtcgtcg
    30421 gtaaagtcac cccacttctg cttcaattcc cccttgaact gtttccattt tccttgtaac
    30481 tggtcctggt tgactgccac cgtgacagtg cccgggtgtt cgtcagcccc atgtgtgctg
    30541 ccgaaggatc ggtcgccggc ctggctcaga cccgatgtca ggaccatgcc tgtcagagtc
    30601 atcgaaagca gcctcatcct gagtttcatg gcctctctcc tccgctacat cgcgtggcgc
    30661 gcgccggcct gcgcggccgg cacgcccgtc tccatctagt gatcgatttt cgtttgacca
    30721 ctctgcgcat cgacatacac cttctgcgtc ttgttgttgg cgtcgacgat ctccacttcg
    30781 tagacggttc ggccgtcttc ctttccgatc tccgcttcga cagccttacc cggcaccgcc
    30841 ttcaacgcgt gccggacggc gtcttcaagc gtgaccgtcg cagtggaggc caactgcttg
    30901 ttggtttcga acaatgccca ggcgggactc gccaggactc cggacatcaa cgccattgca
    30961 ataatttttt tcatctcgaa tgctccttgt tatgcttgtt atgattgatc tgatactcaa
    31021 tgaagacagg cccaccgtca acgctggggt tgagcctggc gtccttcggt gcgcggcttg
    31081 ccctgttgcg cgtaccagtc gtcggcccat ttcaccactt cttccttctt gtcgccgtag
    31141 cgttcctgga cccgcccgac aaatttgtcg taatcgcctt cgacctgcat caggtcgtcg
    31201 tcagtgagct tgccccactg ttttttcact tcgcctttga actgcaccca ctttcctttg
    31261 aattgatcgg tattcatcgt gatcctcctt gttgtgatgt catcgctgcg gaagcaggcg
    31321 acgctgatat cgccgcatct ccgcctcgac tttgctgggc ggcctctcga aacacccggt
    31381 ggtccggagt gcggaaggcc aaaccggtct taccgccacg gtaaagcacg acggtgttcc
    31441 tgccgtcatc ttcaccggtc ctgacgacga ccgtctgctt gactcctttg aatttcagat
    31501 gtttcatcgg cgcctccatg ctgtacatgc ttgttatatc tctcagggct tccggccagg
    31561 actggacata cccctagttt ttacagcggc ttaccccgga cgatcgcatc cgcatatacg
    31621 tacttcatct attccttcgc acgatgatga ggtcgagcac actcggctcg gccgatcagc
    31681 cggccggcac aaacatagac atgacgttgt tcgtgaataa gggcagtcgg cccgggtgcg
    31741 gaaggatcac gcgccgtgta cagactcccg gcaaaggatc gccatggagc gggcttcgat
    31801 ggtgtatggc tcgccgccct tgaatacagc gctcgatcga cgaggtgtag gatcgcaggt
    31861 atccaacatc aggacccagg aggtgcgctc atcgcatgcc ggcaggacaa atggaataga
    31921 ttcatgatgg gcgttgaaga gtaagacaac cgtatcgtcg acgatggcgt tgcctcgtgc
    31981 atccttttct tcgatggcgt cgccggcgag gacgaggccg atggctctca agtgcccctg
    32041 attccaatcg gcctcatcca tttctttgcc gtccgagtgc aaccagacaa tgtctgtgat
    32101 gtcggacccg tggatccgcc gcccttgaaa gaaacgccgg cgacgaaaca ccggatggtc
    32161 ctgccgcaag gcaatgagcc ggcggacgaa cgtcagcaag gtccggttct cccggctgag
    32221 gtcccagttg tgccaactga tttcattgtc ctgacagtag gcattattgt tcccgagctg
    32281 cgtccggccg atctcatcac cgccgcacaa catcggcacc ccctgcgaga ggagcagagt
    32341 ggccaacatg ttgcgtttct ggcgacgacg gagttccagc acgtcccgat cctcagtcgg
    32401 tccttccgcc ccgcagttcc agctcaaatt gtcgttatgg ccgtcctggt tattctcgcc
    32461 gttggcctca ttatgttttt cgttgtagga cacaagatcc tgcaagctga atccgtcatg
    32521 cgctgtgatg aagttgatgc tggcaaacgg gcgtcgcccg ctcatctcgt agagatcgct
    32581 gctgccggaa agccgatatg ccagctcggc aacctgccct ccgtctccct tccaataccg
    32641 gcggatcgca tcacggtact ttccgttcca ttcagcccag ccgacaggga aattaccgac
    32701 ctggtatccg cccgccccga gatcccaggg ttcggcaatc agcttcacct gcgacagcac
    32761 cgggtcctgg tgaatgatat cgaagaaggc gctgaggcga tccacctcat gcagttcacg
    32821 ggccagcgtg gaggccagat cgaaccggaa cccgtccaca tgcatgtcca gcacccaata
    32881 ccgcaaactg tccataatca actgcagcgt tctcggatga cgcacattca ggctgtttcc
    32941 acagccggtg taatccatgt agtaacgcgg atcgtcgccc atcagcctgt aataggcggc
    33001 attgtcgatt ccgcgaaacg acagcgtcgg cccgagatgg ttgccctcag cggtgtggtt
    33061 atagaccacg tcaagaatca cctcgatacc ggcgctgtgt aacgtcttga ccatcgtctt
    33121 aaattcacgc acatgccggc ctcgaaccgg agacagggca taccgcatgt ccggcgcgaa
    33181 gaacccgatg gtgttatacc cccaatagtt cgtgagtccc tggtcggtca aatgtttgtc
    33241 ctgcaccgca tggtgtaccg ggagaagctc gacggctgtc acacccagcc cctgcaaata
    33301 ttcgatgacg ggcggggtcg agagtccggc gtaggtaccg cgcaaatgct ccggcacatc
    33361 cggatgacga gcggtaaatc ctttgacatg cacttcgtag atgaccgtct tcgaccaggg
    33421 tgtacggagc agatggtcgc ctccccagct gaatgcttga tcgatcacca cacacttcgg
    33481 actctcgtcg gcattgtcgc gatcgtccct cgccagatct ccctccgggt cgccgatgcg
    33541 atatccgaac atgcgatcgg acaatcggat cgtgtcggcg atggatttgg catagggatc
    33601 gatcagcagc ttagaggggt tgaatcgatg cccggcgccg ggctcatagg gcccatccac
    33661 acgataaccg taatggaggc cggggcgacc ctccggcagg taggcgtgcc aggtttgatt
    33721 ggtccgttcc tccagccgaa tccggtggga ttctttcgaa gcatccggcc catcgaacag
    33781 gcacaactcc accgacgtgg cattctccga aaagagagcg aaattgaccc cctccccgtc
    33841 ccacgtggct cccagcggat agggtcggcc cggccatacc ttcatcatgt ctccgtttca
    33901 atcacagggt gaaccgaggc aaccgtcgtg ggaaaccggg tcatttcagg agactgacca
    33961 gatcttcggc atgctcttct tctttcatca gaatttcttc cagcaacctg cgcgtggtcg
    34021 gatcatcgct gccgacgaag gcgatcatgt cccgataact ctcgatcgcg attcgctccg
    34081 ccaccagatc ctccttgatc atgtcttcca gattctcacc ttcgatgtat tcggaatggc
    34141 tgcggtcggc caagccctcc ggtgaaaggt tcggttcacc acccagttgg acgatacgct
    34201 cggccagctg atccgcatgc gcctgctcct cgcctgcgtg ggccagaaat tcagacttca
    34261 cgctttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntat ggggccgcaa
    34381 gatctacacg cagatggggc aaagcggact ctaaccccag ctcggcggcc ggctcgacgc
    34441 ccggatcgcg gtcgttggcc ggttgaagca ggcacttcga ctgggtaccg accaccaatg
    34501 cgcaagcggc cagcgagaga aacagaccga cttgttttcc ggttgtatca aacggcatga
    34561 cctgttctcc tctctcatgg atctcaattt gcctgtgcgt agagtagagg cagatggccc
    34621 cggagacgta ctaggaggaa ccctggacag gcaattcaac tgccctggaa accgaagcgt
    34681 ttttttgatt cacactagga ggcgtggacg gaaagcactc cgaccagctg ttcgtggcga
    34741 ttcatcaccg gtaaccagtc cgactgcgac ccgtgcatga ccgaaatcgc gtccgccagc
    34801 aaatcatctt ccagacagca gggcacagac gttctcatga atccagaaat gggggcatct
    34861 tgaggggctt cctgcactcg gcggttcaag ccgatgtctc gatcagtgag cattccgacc
    34921 agccgtctgc cgtcatacac cacgacgacc ggcacgttga gcgaggtcat catcgacatc
    34981 gcctccttca gacttgtctt aggagaggcc accgtaaccg cggagctcat cgcgctcttc
    35041 accttccgag cctcgcccgg gagcaacccc aactgctcat ccagcgtgga ctccaggtat
    35101 aaactggacc ggtctcgtct cgccctggcc tcctccattg ttctaggacc ttcgtgtttc
    35161 attccagacc tccctctttc actcacacac actaaaccgc ctgcggacga acttcatgca
    35221 ccgtgagtcg aagtctgcca tactcaaccg gcttatccgg atttctggtg tatttcgttt
    35281 tgtcttcacc gatcgagact atgcttaaag gaagcgtgag gtgctgtttg taatgcggga
    35341 atgcggtttc gagcaggagc gtgacggttt ggctttcaca ctcgatcact tcggcattca
    35401 agtcacgctc atcctcaaaa tccaccgtga tccgctcctc gggatggatc caccgacgga
    35461 tcgtcatgac cttctcatgg gaatccatgc ggtgcctcct cgtggcggtt tgttcgagcg
    35521 catgctgccg ttcagcacag tgacaagtgg tatcatctca cgcacagtgc ttcccgccca
    35581 ctagcgatgt ctctaggcca tctccgaaac cacagggacg gggtcccggc acatgaactc
    35641 ctgttccagg agctgtggaa tgggcggcct ggtcggcaga ggcggaatcg gagggggaaa
    35701 gggttgcgga ggaaacgggc ctgggtccgg cccggggaaa ggagccggat cgggcggttg
    35761 cggatctgga tcgggtctga tgacgagcag tttcatagtc acctcctcat cgcgggacga
    35821 ccggggccga acaggcggcc tgcagtcgct cctccactgc gctccaagag atattgctga
    35881 agaacgcctc aatatattgc ttccgttcct ggggttgata atccagcaag taggcatgtt
    35941 cccacacatc catgacgagg atcggcagga agccggcaac atttccatgc tcatgcagcg
    36001 agacccaatg gttggagaca aggccggtgg agggatcgat attgcagatc gcccagccca
    36061 cacctcgcaa catgcccaca ctgcaaaagt ccgtcttcca acgatcataa ctcccgaagg
    36121 tccgctcgac tgcctggccg aacggcgacc cgtccgccgg acgatccatt cctcctcgtc
    36181 tgagattgcc gaagtaatat tcgtgcagga tcattccgtt atattcaaac cccaggcgcc
    36241 tcgtcagttc ggagtaggcc ggcatttctt cttgatccac cgtcccgtcg ctgagtatct
    36301 catgtaagcg ggctcgcaac gaattcgccg ccttgacata cccttcgtac agcccgaaat
    36361 gaaccgcgag actgtgatct gaaatgcctg acaatccgaa cagatcgtag gattttgctg
    36421 tatacgggtc gatcttcctc atgtgtccct ccattacatc acgtatgcat c
//
LOCUS       DAOQRM010000008        35245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_640_length_35245_cov_15.660983, whole genome shotgun sequence.
ACCESSION   DAOQRM010000008 DAOQRM010000000
VERSION     DAOQRM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 35245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35245
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_640_length_35245_cov_15.660983"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(396..872)
                     /locus_tag="PKK81_01580"
     CDS             complement(396..872)
                     /locus_tag="PKK81_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNV31586.1"
                     /translation="MKCQSTRFGTFEVNDDTLLVFPSGILGFPEWSKYVLLDHDTEAP
                     FKWLQCVEEPQLAFVILDPDFFKPDYQIEVSLDALIEIQKQDSDELSVVTILTIPSDD
                     PTAVTANLRGPLVMNHRTRLCKQLVLSEDWPTRYPLFSAPPAQRPTPTAPLQATAG"
     gene            complement(966..1127)
                     /gene="csrA"
                     /locus_tag="PKK81_01585"
                     /pseudo
     CDS             complement(966..1127)
                     /gene="csrA"
                     /locus_tag="PKK81_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
     gene            complement(1201..2091)
                     /gene="flgL"
                     /locus_tag="PKK81_01590"
     CDS             complement(1201..2091)
                     /gene="flgL"
                     /locus_tag="PKK81_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNV31587.1"
                     /translation="MRVADQQMYGILLGNLQRSRLQMLTSQEQISSQKRVTNPADDPS
                     AFGQIVLDKSALSQTTQWVRNINFGTSRVNAADQALGQVQNLITRVRELTIQASSDTT
                     SAEGRQSIAKEVRQLQRQLVQLGNTEVAGQAIFGGTKTDVQPFTITSGDTVAYQGNSE
                     TQSIAVGENQTVQILVPGSSIFTGSTTNMFDSLRDLLTALESNNRSGIQAGLGNLDLA
                     TAQISDVQGTVGALANRLQVTHDALDTATLTITKSISDNQDADLATAITQLRLQEVAV
                     QAASETFTKIFDSSLINYLR"
     gene            complement(2123..3517)
                     /gene="flgK"
                     /locus_tag="PKK81_01595"
     CDS             complement(2123..3517)
                     /gene="flgK"
                     /locus_tag="PKK81_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNV31588.1"
                     /translation="MSGLNGLFGIGSNALATFQRALSVTGQNIANVSTPGYSRQEITL
                     SESLPENGQPGQIGTGVTATEIRRSVDSFVERQLLSSNERVGQFGASQKALSQIQLVF
                     NDANDQGIAAGLNEFFKAWQDVATNPADLTARTVLLTKADGLTKLLNQADAQLSAQRT
                     SLDGQVQSSINDVNALASKIADLNSQIKLTEISGQHANDLRDQRGRFLNDLAGLVEIS
                     SIEDATGQVTVFVGIGQVLVAEKTAYTLTGVANVANNGLLDVRYDGGTGPNTDITSAI
                     SGGRLKGLIDARDTTAAGLQTSLNTLTSQLVSQVNTQHRLGYGLDGSTTQDFFTASGT
                     AAGTISLALTDRQKIAASSTAAGVPGNNVNALALSNLQTASVAGLGNTTFQGYYSAMA
                     GSFGATLQGATRDLQGQEILHDQLLAHRAEVSGVSMDEELINLLKYQRAFEAASKLIT
                     TSDEMLQTILTLKR"
     gene            complement(3529..3981)
                     /locus_tag="PKK81_01600"
     CDS             complement(3529..3981)
                     /locus_tag="PKK81_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNV31589.1"
                     /translation="MVSTVPQPLSTASDSLLCRMLDKVTAFQALLLEEQDAIRSLSFG
                     QFTSVTMRKTQLLDEMRELEQQRRNEATLSGSYGSPPSLKQQEADLVAAIGQTDRLNR
                     FNAALIGQSLEFLQGTLRIWQRSPQSAALYSSSGAAVAGNGGRVRAKG"
     gene            complement(4055..4360)
                     /gene="flgM"
                     /locus_tag="PKK81_01605"
     CDS             complement(4055..4360)
                     /gene="flgM"
                     /locus_tag="PKK81_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNV31590.1"
                     /translation="MEISHNGRAADLAKILLGVQDTERTHNKKTASQGTQSQDRVQIS
                     EQAKELQRLRAAAEQPDAERDARVGQIRQSVEGGTYNVDGKKVADAIIRNVLTDAVL"
     gene            complement(4465..4776)
                     /locus_tag="PKK81_01610"
     CDS             complement(4465..4776)
                     /locus_tag="PKK81_01610"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNV31591.1"
                     /translation="MSSQLDLAQMATPQPLGFRGPVEQAPAGDKARAALHKAGQEFEA
                     YFISYLIKNMRETVPKGLLDRKSEQVWYSFYDQEIAKLSTEAGGIGITAFVDAYAEKL
                     P"
     gene            complement(4813..6123)
                     /locus_tag="PKK81_01615"
     CDS             complement(4813..6123)
                     /locus_tag="PKK81_01615"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNV31592.1"
                     /translation="MTRPRKQQRAMITQCFRLMLSSGVLRPASMDPGLLTRPWTPPPL
                     KPAPVPERQPQGTAWFRRTVVGMLAFLTITLFTASLAQAVRVKDVATIEGVRENQLIG
                     YGLVVGLDRTGDQVIGGQFTIQAMMSMLNKMGINLVIDPIQLLTRNIASVMVTAKLPP
                     FVKPGMTLDAVVSSMANAKSLQGGTLLSTPLKAPNQQVFAVAQGPVSIGGFLGGTGGA
                     GGATVTKNHQAAGVVPAGAIVEKELVVDIENWDSVSVLLRHPDFTTAIRTAEAIDGTF
                     GKGTAVPVNAGLVKTSLPATFHGRVVEYIATMEGLDVTVDVAAKVVVNERTGTVVLGE
                     HVRLSTCAISHGNLTISVKNTLNVSQPPAPLIGSTQGQTTVTPDVQTEVTEQESRLIV
                     VDQTVTLGEVVRALNAVGVTPRDLVAILSALRSAGALQANLEIV"
     gene            complement(6167..6883)
                     /locus_tag="PKK81_01620"
     CDS             complement(6167..6883)
                     /locus_tag="PKK81_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNV31593.1"
                     /translation="MSVFNRSFMQCRPIGFIWCGLALCVLVGCSASPSTKQQKVEMAK
                     LPPPKTTGSLWQEENGRAYLYEDLRAMRIGDIITILIVEKHKGSKSADTNAERDSTIS
                     NGIGGTGMGYLGIPGIRLGGEAKRGFGIDASAKSKFGGKGATNREDTLTGTISAIVTE
                     VLPNGDLRVEGRREVTVNSERQIMTIGGIVRRVDVNTKNTVQSSAIADAKIEYSGLGV
                     VDDVQRPGWLVRILDWVYPF"
     gene            complement(6870..7667)
                     /gene="flgA"
                     /locus_tag="PKK81_01625"
     CDS             complement(6870..7667)
                     /gene="flgA"
                     /locus_tag="PKK81_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNV31594.1"
                     /translation="MNRLSLLSVATISLVTSAVLPAMAGERAPVRPLFQVQGAVAHQA
                     QGLPQARGKRLIYPEQIRGVIHDFVKRELAGRAVDCQVAIGDPQQPIVVPSGTVDLQV
                     SAGRSDEPLGRRVFQIHLAVNGRFIKTVDATADVAAIVEVVVPVRSIKVDEEIAADDV
                     TTERIVLYDLKQPFVTSPAEVIGKAAIRPLPPQNAIRMTSVRRPFAVHKGDRVTIEAR
                     QGGLSIQTVGVTKSHGELGQTITVSNVDSGKELRATVVAPGVVRVSF"
     gene            complement(7701..8492)
                     /gene="flgG"
                     /locus_tag="PKK81_01630"
     CDS             complement(7701..8492)
                     /gene="flgG"
                     /locus_tag="PKK81_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNV31595.1"
                     /translation="MIRAMWTAATGMTAQQLNVDTIANNLANVNTNAFKRSRAEFGDL
                     LYQIQRLPGTNASNVGVFPVGVQVGGGVRPITVAKEWVQGNMRQTGNDLDLAIDGAGF
                     FQVTRPDGTIMYTRNGSFKRDNVGNLVTGDGDQLNPVITIPSGALKIDVGQDGTVSVL
                     LPGVTQASQVGQIQLVRFDNPSGLVAMGGNLFLDSFASGPAQQGTGGFSTGFGTLQQG
                     FLESSNVNLAEEMVNMIIAQRSYEINSKTIQASDEMMQIANNLRR"
     gene            complement(8502..9269)
                     /locus_tag="PKK81_01635"
     CDS             complement(8502..9269)
                     /locus_tag="PKK81_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HNV31596.1"
                     /translation="MNRAIYPILSGAVAQEKQLTVFANNLANVNTAGFKQDQQGFRGL
                     FARASSTGMGVVSGGLSSAISTRPAGPSERVFAEVHGVRTAFEPGRIRITGNPLDVAI
                     QNDGFFEVKTPDGVRYTRNGIFSLDSQRRLVTNLGHPVMGTKGEIKVPPGNIQINAEG
                     AIQVDGTPIGSIKVVEFPENAMPQKFAEGLFVGGKPTVSTRPQVQSGHIEESNVNSLS
                     EMVKMMQGMRSYESAQKLIQTMDRMTETAIQDLGRVQ"
     gene            complement(9548..10423)
                     /locus_tag="PKK81_01640"
     CDS             complement(9548..10423)
                     /locus_tag="PKK81_01640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV31597.1"
                     /translation="MHSSRPELNTSSDRNGSDSTNRHGWPDDEAGTESLQSARLGVVA
                     CILFMAGGLYWTASVGLLTLSALTAYPVMISAGILLTLMVFGATLWTPRGPQSASRMH
                     SLSEESNGSGMNTYDSVTGLPTYRLFTSLLNQALVHAHKYGRPVAVLMIELDHFTPKT
                     DEQAQINLNLMYRVHAARVKSALRTTDTVARIAERTFVVLLDQVTGPEEVLAIARKMQ
                     LTISLPVTLDGHELFLTSRIGISLSSQDTMDSDALLDVATRAVATARAEGYALYGLPG
                     AMVSASVDATSTIAA"
     gene            complement(10583..11344)
                     /locus_tag="PKK81_01645"
     CDS             complement(10583..11344)
                     /locus_tag="PKK81_01645"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNV31598.1"
                     /translation="MMDARKMSAVRNAPRRVIAEADRERVIQEFTHVVKAMAHRLAFR
                     LPAYMDAEDLMSVGIMGLMDAMDKYDPTREAKFKTYAEFRIRGAMLDEIRSMDWIPRS
                     VHERITLLQKTYSELLHRLGRPPTDQEVGKELKMSTEELDEFLTRSRGAVVISLDDLG
                     VQDADGHKIISVLTDSNQPDPLSVLVSERARHVLEAAIQDLPEKERLVLSLYYFEELT
                     MKEIGQALKVTESRVCQIHSKAILHLKARLEPVDV"
     gene            complement(11363..12229)
                     /locus_tag="PKK81_01650"
     CDS             complement(11363..12229)
                     /locus_tag="PKK81_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNV31599.1"
                     /translation="MAMQPGILDPQMRALDDNVGPSRTQVITVTSGKGGVGKTNVVAN
                     TAIALAQTGKRVLVLDADLGLGNVDILLGLTPKYTLEHVLAKTCRLEDIALTGPHGIT
                     VLPASTGISQLTILTEAQQLILQDELERLASNMDVLLIDTGAGISSTVTYFAAAAQSI
                     IVVVSPEPTSMTDAYALMKVLLRQYRERRFHVLVNMVKSPRDAARIFRKLELAVSRFL
                     HISLDYLGAIPLDDYVPMAVTQQRAVIDLFPHAPSSRAFRELTEAVAQLTPPALPKGT
                     VQFLWQQLLRTH"
     gene            complement(12258..13541)
                     /gene="flhF"
                     /locus_tag="PKK81_01655"
     CDS             complement(12258..13541)
                     /gene="flhF"
                     /locus_tag="PKK81_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNV31600.1"
                     /translation="MKVRTFHVKSMHEAIRSIKDTLGPDAVILSTKRVRSWDNGFGLL
                     GGSVLEVMAAIEDDAAVPEAAPLLGEDEQTRAAVTPAPMVPAEESRFQKTLQGAMETG
                     KEQSRPAARQVPGPSRETRATSASTRNNGENQGATFQSFQRVYEDLLAQGVEHATAEA
                     CLRELRETLVEQGSAQRNSSLQLLRTVLLDRVSTAGPLLSAAGEQKIALFVGPSGVGK
                     TSTIAKLATHYGIVEQRSVALITMDTYRPAAVEHLRLYAEVLGIELSVAASCEEARAA
                     IAQAREAELILIDTTGFNPYEPITAQRWRGLLNGAYPMEVHLVLAAGTRVPDLVVSTS
                     QCVDVPGLRLLFTKLDETAGYGGIFEATHRTGIPLSYWGTGQRVPDDLVQAQVDRLGD
                     LLLGGQVRTPAGGTTQSWDRQTTSAVVPGIKTYAR"
     gene            complement(13538..15646)
                     /gene="flhA"
                     /locus_tag="PKK81_01660"
     CDS             complement(13538..15646)
                     /gene="flhA"
                     /locus_tag="PKK81_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNV31601.1"
                     /translation="MANENTSVSTTSLVKHPDIMMSVGVVGILMVMLIPLPRFFLDLL
                     LAFNITLSIIILLVGMQVRRPLEFSVFPSILLMVTLLRLALNIASTRLILLHGNEGAA
                     AAGEVIRAFGTFVVGGNYTVGLVVFTILVIINFVVITKGAGRVAEVAARFTLDAMPGK
                     QMSIDADLNAGLINDKEARQRRKEIAQEADFYGAMDGSSKFVRGDAVAAVIITLVNIL
                     GGLTIGVLQQGMTLAAAAQTYTLLTVGEGLVAQVPALIVSTAAGIVITRAASEVNLGF
                     EISRQVLISPKAIGTASGILITLGLVPGLPHLAFLALGGLTAWMAYQINEQQKTAEAV
                     PTQSSPQVKAEEAGTQVVPLDLMEVQVGYGLISLVDGGQGGALLDRIKGLRRQFAEQM
                     GFVLPPIHIRDNLQLRPNEYAALLKGVELAKSEVMPAHVLAIDPGTAQRGAIHGLPTK
                     EPAFGLPAMWVPEQQREQAQMAGYTVVDPGSAIATHLSELIKRHAHELLGRQEVQGLL
                     DQLGKNHPKLVEEVIPNLIPLGTLVRVLSHLLREGVPIRDLRTILETIADHAATTKDA
                     DILTEVARQALGRTITKQYLAPDGSLPIIGLDPRLDRTLADQANAAGQGTQWVPDPLV
                     AQKLLTALKAAAERMVGRGHQPVILCSPSLRRHLRKLTDRILHSVPILGLNEVESVTR
                     LQAVETVRLDLEVGETRSLA"
     gene            complement(15669..16751)
                     /gene="flhB"
                     /locus_tag="PKK81_01665"
     CDS             complement(15669..16751)
                     /gene="flhB"
                     /locus_tag="PKK81_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNV31602.1"
                     /translation="MADSGQNRTEQATPKRKADARAKGQVALSRDAAMAVGLLGSLGA
                     LYWMTPGILNKLRESLQLWLSKSMEESTQRTLSLDHLHLILRQIGIDVFIMLGPVIAG
                     IAVVGVGANLMQTGFLWKRDGLQLEFSRISPMGGFSRLFSVRSFSELVKSWLKIFAIG
                     GVGYLTIKQDMLLFSPLTQFGMETLLPTVGWATFKAALMMGGAALVIGGLDYGFQRFE
                     WERDLRMSRDEIKEESRAAEGDPGLKAKIRSTQREMSRKRMMAEVPKADVIITNPTHL
                     AVALKYDSKAMGAPVVVAKGAGFVAEKIREIGRQHGVMIVENKLVARTLFKLVEVGRE
                     VPEDLYRAVAEILAFVYRVRGKLPPA"
     gene            complement(16744..17532)
                     /gene="fliR"
                     /locus_tag="PKK81_01670"
     CDS             complement(16744..17532)
                     /gene="fliR"
                     /locus_tag="PKK81_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNV31603.1"
                     /translation="MSAAQTLHLALPQFQAFSILLVRIAGIVSVFPILNTRTIPMPVK
                     TGLVATLGLVLAPIIHLPSLPTDPALVIAGIGSEFLIGLTIGLAVRLLFSGFQVAGDM
                     IGTQMGFSAIQMFDPMSHQNTPIIAQFQLTLGSLVFLSLNAHLLVVHAIGMSYEYVPP
                     FGASLSDGIAQDIVHLAQNMLGVALKLAAPVFATLLIVNTVLAILGRAVPQMNVFVTS
                     FPITIGAGFLAMSLAMPFTLLLFEKEFETLVETILGLLKALGHG"
     gene            complement(17549..17818)
                     /gene="fliQ"
                     /locus_tag="PKK81_01675"
     CDS             complement(17549..17818)
                     /gene="fliQ"
                     /locus_tag="PKK81_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNV31604.1"
                     /translation="MTIETVTEIGRQAIETTMLVSAPILGLSLIVGLLVSTFQAMTQI
                     NEATLTFVPKVLAVFAATLLFLPWMMGVLIAFMTHIITSIPSLIH"
     gene            complement(17829..18629)
                     /gene="fliP"
                     /locus_tag="PKK81_01680"
     CDS             complement(17829..18629)
                     /gene="fliP"
                     /locus_tag="PKK81_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248836.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNV31605.1"
                     /translation="MHTASLLSPPREASAAQRSGWWAGLLISLVVLACLFSVSFAYAE
                     GPSLTIDLGQGGSKQTAVVLQILALFTVLSLAPALFIMVTSFTRMVIVLSFLRQAMGT
                     QQVPPNQVLISLALFLTMFVMAPVGQAVYKDALQPLLAEQIGYEDAWNRGIQPIRAFM
                     LKQMRDKDLELFMELSHAPKATQVEQVPIHVIIPAFVLSELRISFQIGFLLYIPFLIV
                     DMVVASVLMSMGMMLLPPVMISLPFKLILFVLADGWYLVVGSMVRSFQ"
     gene            complement(18616..18984)
                     /locus_tag="PKK81_01685"
     CDS             complement(18616..18984)
                     /locus_tag="PKK81_01685"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNV31606.1"
                     /translation="MEIWESVVRMVSALGVVLALILGLLAVARSAVGRRFLPVNGAPL
                     VKILGSGPLGSRKHVMVVAVAGEVLILGTTATDLVPLGKIADPDVAKRLIAEASVLPS
                     GAAIGIPGSGNLEETARAHS"
     gene            complement(19042..19416)
                     /gene="fliN"
                     /locus_tag="PKK81_01690"
     CDS             complement(19042..19416)
                     /gene="fliN"
                     /locus_tag="PKK81_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNV31607.1"
                     /translation="MGNGDTTAPSDASNAGAGDINVQPTSFPPVDNQNAVPANKNIEF
                     VLDIPLQVTVQIGSTRMLIRELLQLGQGSVIELNKLAGEPMEVLVNNKLVARGEVVVV
                     NEKFGIRLTDVVSPNQRIQQLG"
     gene            complement(19446..20417)
                     /gene="fliM"
                     /locus_tag="PKK81_01695"
     CDS             complement(19446..20417)
                     /gene="fliM"
                     /locus_tag="PKK81_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNV31608.1"
                     /translation="MDKILSQEEIDALMGGVMSGEVETAPKEEEDPGGVKTYSLTSQE
                     RIIRGRMPTMEMINDRFTRQQSISWSAVLREKIEFSVLGTQIMKFGDFIQKVPVPSSF
                     NLFAMEPMRGNGLYVMDAMLVYLIVDFFFGGKVQTHVKPEGREFTTIQVRLIKKLVQQ
                     ALADLEKAWQAVMPVKIGYLRSESNPQFAMVVTTSEIVVVVTLQVHLGDISREMFIAY
                     PYSMLEPIKEKLYSGLFADNVEQDSSWGSRFKDSLQECDVAMTVQLGTARINVQDLLN
                     FNPGDVLMLDQHPGDPMLCLVEGDPKFQGQPGIFKGNHACRVTKILG"
     gene            complement(20442..20978)
                     /locus_tag="PKK81_01700"
     CDS             complement(20442..20978)
                     /locus_tag="PKK81_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNV31609.1"
                     /translation="MSEATEEKAPAAPAGIPMKMVIIIVAGVLVLGLGGAFAMFKMMG
                     GPSGEKAHSENGGEKQVAHGEAEDKGGGHGAVADASAIADLDPFIVNLADTPEIRYLK
                     VTLKVEADSSNTVEQIKARTPQIRDAILVLLTGLDSATARSPQGKHKLREDITDRING
                     LLPKKSVKSTYFTEFVIQ"
     gene            complement(21312..22535)
                     /gene="flgE"
                     /locus_tag="PKK81_01705"
     CDS             complement(21312..22535)
                     /gene="flgE"
                     /locus_tag="PKK81_01705"
                     /inference="COORDINATES: protein motif:HMM:NF004238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNV31610.1"
                     /translation="MGILTSMFTAVTGLSSYGNAMGVIGNNIANVGTAGFKSSRATFS
                     DLISSSLGGGSGSDQIGLGVFLNDVQTNFSQGSMTTTGNTFDLAIDGNGFYLVRNTSG
                     ANFYSRAGQFKVDSVGQVVDSSGALLQGYQADTNGNITSTIGGITLTSSAVPPVATTS
                     AEILGNLNANATAPTTAFSVTDTTSYNFSTGMTFYDSLGNSHQMQLYFRKTAANAWGV
                     YSQIDGGAAAAQTNMTFNASGAVTAGGTQTVTATLTNGATTPQAMTVDLSSLTQFGAA
                     SGVASQTQDGYSSGTLDRISVDKEGRVMAQFTNGETRALAQVVLSRFTNPDGLVNVGD
                     NHFLETVESGAALAGAPTVNGLGRVLSGTVEQSNVDLGKEFVDMIITQRAFQANSRAI
                     TTSDEMLQELVNLKR"
     gene            complement(22609..22899)
                     /locus_tag="PKK81_01710"
     CDS             complement(22609..22899)
                     /locus_tag="PKK81_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNV31611.1"
                     /translation="MDISKVITQATPPDSSTPTTDGPKPLGKDDFLKLLVTQLQHQDP
                     LKPMENEDFVAQTAQFSQLEQMSKLVSLMEKSVTLQENAQNKTATTGTTAVA"
     gene            complement(22911..24170)
                     /locus_tag="PKK81_01715"
     CDS             complement(22911..24170)
                     /locus_tag="PKK81_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNV31612.1"
                     /translation="MKADCVSDTEVESPNAEDNTEQAEDQSTQRQEILVAALLLPTPA
                     VAQTPVQISGETDSELDPSADGAGKVAPTAATGPHETPSAQLAALDPSVQTSEEALVG
                     QAATAQAQTSDIKESAEKEQPGEGMIDMSAFPHPDSHGEAREVSADRTDAAVTASTID
                     PEKVTTVAAAVTTRTAEAGGMKQADGDRVDRPAFLEDLAQDSPVSTEQGSANAGKFLE
                     NESEPFSDSSADADRSMPNKAMSNGPIDRSLFLDRMSGLNQPAPPPSESAVGRNEAGQ
                     PAAVLRAAEAERMSELRGASPVSQSVMLDLDPLDMGPLRVRVMMSDQTVHAHIRTEHG
                     ELGQGLLQQGQSLESSLRTTGLEMGMLRVTVDQQQGRNDNAWMFQQQHQDRPTTSSGQ
                     RSMTGEEERSARGETGDYTNERVSIFA"
     gene            complement(24684..25253)
                     /locus_tag="PKK81_01720"
     CDS             complement(24684..25253)
                     /locus_tag="PKK81_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31613.1"
                     /translation="MALLGGMGTAVVADEGAPAPAPAAAKEEPKADAIKKEVQGPAIS
                     TPREIFQMLEQRKRALDKREAALRGSEMHLLELKAELEQIVTRHEQAVEAEKKRRQAA
                     QNKATSEAEKPKPTAKPGGAANVNQTQLAKIYEAMPAEEAAARLERMPDRKALEVLRL
                     IKGKSAGAILAEVKPERAARLTEQLLTAP"
     gene            complement(25319..25759)
                     /locus_tag="PKK81_01725"
     CDS             complement(25319..25759)
                     /locus_tag="PKK81_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HNV31614.1"
                     /translation="MNVTVLRTYAIQLEEVAKLELAELASALQQTVDRMTMLDSRARA
                     DADQYLLQVRQGSTVDQVYGHLDAMDQAIAARKSMEQMQRDQQVKMEDKRGELLEAMQ
                     YRKKLDLLDARAALERRRRRERQDQQLLDERAWRRSPLQQGERA"
     gene            complement(25756..27063)
                     /locus_tag="PKK81_01730"
     CDS             complement(25756..27063)
                     /locus_tag="PKK81_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNV31615.1"
                     /translation="MKLSERLEQVEPLSVTGRVAQAVGIVIEASGPFTSVGEVCEITR
                     EGGHGAVMAEVVGFREDRVLLMPLGEMQGIGPGSRVVMKGHVASVSVGPQMLGRIFDG
                     LGHPLDGLPPVRTDVQVPLYAPPLNPLHRARITQPVDLGIRAINALLTCGQGQKIGVF
                     AGSGVGKSVLLGMISRYTQADVRVIALVGERGREVKEFLERDLTPEARARSIVVVATS
                     DQPPLVRIRGALVATAIAEYFRDCGKHVLLMMDSLSRLAYSQREVGLAIGEPPTTKGY
                     TPSVFAVLPKLLERVGPAQSGGSITGLYTVLVDGDDLNDPVADAVRSILDGHIVLSRE
                     LAARNHFPAIDILQSTSRVMRDIVTPAHYAAARLVLERTALYRRSEDLITLGAYKPGT
                     SKELDKAVSAHEGITAFLRQETKQSVGLSQSIDALLALAGTIQ"
     gene            complement(27053..28102)
                     /locus_tag="PKK81_01735"
     CDS             complement(27053..28102)
                     /locus_tag="PKK81_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31616.1"
                     /translation="MRTVDVGSRLPQMSPADNEKELAPRAILIVDDEPSIGFLLTEML
                     QSTGHPILTAGSVSEALTHLKSRPIAVLIVDVQLAGTDGIAFLQQALEVDSRLLSIVM
                     TGHGNIELAVRAMKSGAADFLTKPFEVELVRQSVSRLLELYRLRQENTVLTRTLIRSG
                     NIQLRTVPLADFSRGNRPFGTDDVTEFERGLAEGEKRATERVSSLRQREQALVASLMA
                     QLEGSWRNLHDTVEEEVASLSFMIAQKVLRELVTEKREVIVTQVRTALAHLHESGLVR
                     IRVHPSDLPLLEASRAALSQTPHGLLTLKFETDPGISPGGCLVQAQSLLIDATLDTQL
                     LRLGEALRKRDTGEA"
     gene            complement(28103..29098)
                     /gene="fliG"
                     /locus_tag="PKK81_01740"
     CDS             complement(28103..29098)
                     /gene="fliG"
                     /locus_tag="PKK81_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNV31617.1"
                     /translation="MNKELSGAQKAAILLRAIGEEAAAAVMKTLDPKDIRKLGSFMKE
                     TANITKQEEDSVIAEFEQASSSGEVQFEGREFMEAILKKALGPEKAARMMESLNTKTY
                     PGIDALKWVDPRTVAQILKIEHPQTIAVCLGQMEAEQASAVLALLPVHLHADVSLRLA
                     TMQEVQPEVLSELSDSLQEILSASMGMSSMSVGGAELMADILTRVDKNTEGAIMAKIT
                     ERDQALAESIRALMFVFDDLVELDSRGMQELMKEISKEDLPVALRGATPEIKEKFLKN
                     MSSRAAEMLKEDMETRGPVKVSDVEKAQQNILKVCRKLEEEGRIVIGGGGGEELL"
     gene            complement(29110..30666)
                     /gene="fliF"
                     /locus_tag="PKK81_01745"
     CDS             complement(29110..30666)
                     /gene="fliF"
                     /locus_tag="PKK81_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNV31618.1"
                     /translation="MFSKFSQFTINQRFIILLALAGSIAGLVAVTLWTQQPDMQVLFA
                     NLAVDDASGIIDKLKDAKVPYETANGGTTVLVPSAQVHDLRLEMAGQGLPHGGGVGYE
                     IFDRTTMGMSDFVQKLNYRRALQGELARTITQMPEVERARVHLAIPERRLFATEQDRA
                     RASVVVSLRASQTLSKAQIQGVVHLVSSSVEGLQARDVTVVDGHGNLLSNTSSDESAG
                     LSGTQMEYQRTLEKDIETRIQTMLERIVGVNKAVVRVSSVLDFRKIETTEERYDPNGQ
                     VVRSEQRGQEKSSGVNGTSGGVPGVESNVPGGTEAEGGQTSSNNNQTKNETVNYEISR
                     TVSRIVEPTGTIKKLSVAVLVDGTYEGGGKAGEGGADQPKKYVPRSEEEMKRIEEIVK
                     KAMGYSTERQDQVEVVSIQFGLGAEEPAGGVEATPDASKAWMPYVRYAVGGVLFFLIF
                     FMVVRPLMVMLVQSAPAAGGGETPALPASVGQVEAAISGKQPGQILDMAKNNPANTAV
                     VVKQWLKSNA"
     gene            complement(30716..31027)
                     /gene="fliE"
                     /locus_tag="PKK81_01750"
     CDS             complement(30716..31027)
                     /gene="fliE"
                     /locus_tag="PKK81_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNV31619.1"
                     /translation="MSDLRIAGANGFRPIEMPEIHEAGPGGEAGAANFMGSLKEAIGH
                     INDAQTGASQAVDALVTGQSTNIHQTMVALQQADVSFQLMMQVRNKLVTAYEEIQRMQ
                     I"
     gene            complement(31030..31479)
                     /gene="flgC"
                     /locus_tag="PKK81_01755"
     CDS             complement(31030..31479)
                     /gene="flgC"
                     /locus_tag="PKK81_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNV31620.1"
                     /translation="MELTDSLAVSVSALDAHRHRLNVIASNLANAQSTKTSTGGPYRR
                     RDVVFQAAPVSSAFQKAFKQVTSGPGRQALDGVKVARVIEDKKPGQTVYDPRHPDADK
                     KGFVTMPNVNVMEEMVNMIGASRAYEANVQAINATRTMWNRALEIGR"
     gene            complement(31540..31947)
                     /gene="flgB"
                     /locus_tag="PKK81_01760"
     CDS             complement(31540..31947)
                     /gene="flgB"
                     /locus_tag="PKK81_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNV31621.1"
                     /translation="MTIFDRTMQLLSRSLDLRGARQQVIAANIANEETPKYRAKDLNF
                     GQALASAQQGKLPISLVSTNAQHFGPQGTGYQRVHGQLEDVPAGDLPLDANSVNIELE
                     MAKMSDNAQQYNTAATIIGMKFKGLLGAIREGR"
     gene            complement(32117..33535)
                     /locus_tag="PKK81_01765"
     CDS             complement(32117..33535)
                     /locus_tag="PKK81_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV31622.1"
                     /translation="MSVQQDLQGPSATSEEARMVLIVDDEPSMRTALSETVRRLGYQV
                     RGAIDGVDAIEQIERVKPWLVVTDLKMPRLTGLELVKAIKQKSPQTFIVLMTAYGTVE
                     TAVEAMKYGANDYILKPFSTDLLERVILNLQATTAPDEQEGPATLEARAILTQDPGMI
                     RLLTTLEGVAASQATVLISGESGTGKELLARYIHARSPRAHRPFVALNCAALPDSLLE
                     SELFGHERGAFTGAIQKKLGKFEMAHTGTLFLDEISEMNLGLQSKLLRVLQEREVDRI
                     GGREPVPVNIRVIATTNRSLYHEVTQGRFREDLFYRLNVFPVTVPPLRDRSGDIPLLA
                     RHFLRSSAQRNGLTMPTLSERAIADLQQRPWKGNVRELENVMERAILVATGGAVDVDH
                     LMPGDGTVPVREVEAGDAIVTPSAHGSLWEMERDLIFKTLARVKENRTHAAKELGISI
                     RTLRNKLREYRDMGYQVEAEKS"
     gene            complement(33532..34821)
                     /locus_tag="PKK81_01770"
     CDS             complement(33532..34821)
                     /locus_tag="PKK81_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV31623.1"
                     /translation="MTARDAEASNNDLLTRAFHDFDQAATVLQQSYDALTTRLQQMDL
                     ELAQTNASLREHLRETEEMRAHVTAVLESLDTGVIVADSQDSVVRCNHATERLLGVPQ
                     ARLRGRRATEVLEEIRKDHGEYPLVLPTGVTIAISQSDLTDETGSLIGKLVLIHDVTH
                     IRQLEDRLQRRNRLEAMGQMVGNIAHEIRNPLGSVELFASMLRKDLRDQPHLRTYAEH
                     ISVAVQAMDRLLSNLLVYTRPDCSRLAWQDTESLIREVLTLASHAMSPASIVVRCEVD
                     PRVPKIWCDGGKIKQVLLNLVLNAVQAMPEGGSLILSACMAPEQISELPAIQLTVSDT
                     GTGIPLELQSRVFDPFFTTKDHGTGLGLAIVHALVEAHHGRIDVESRPGQGTSFVMRL
                     PQGSIQQPTLSARNAALVGRSVQTRPIENATEEETYE"
     gene            complement(34818..>35245)
                     /locus_tag="PKK81_01775"
     CDS             complement(34818..>35245)
                     /locus_tag="PKK81_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31624.1"
                     /translation="WLIHNPRHPDRALVQLKVADALRQAKRDAEAAPLYESVLKAGMD
                     LSALDRLRYADVLARLNRQEPALALYKQALIAGLEPEQEAWAQFQIVQLARGVKREEF
                     ARNGLRALNENSDSLVRRMAAVLQTELPEPTSAAGGKKR"
BASE COUNT         6855 a        10748 c        10024 g         7618 t
ORIGIN      
        1 aactggttgc ggtgctggag cgccagatca caccagccga cccggctcag tcgcgctagt
       61 ctattcacag tgcatgggaa agccgcaacg ctcttttttt agcaggatgc ggaaaaagtc
      121 cgccagcggc gtcctcgaca tccgtgaatc gtgaagcgta tctcgtcgcg gtcagagcgt
      181 atcgctggtg gcatacagct gagagtagag atgcccactc tcgatttctg gccatacgct
      241 atgagctcta tgccataagc tcttttctgc agacgagaga cgaacgacgt ttcacgaacg
      301 acggactatg aagacggcct ttttgcgcat ccagcgggtt attgtcatac cgacacggcg
      361 cacgctgagc ccggtacgcg atgagtgatg cgtgtttagc cggctgtcgc ctgaagcgga
      421 gccgttggag tggggcgttg tgcgggggga gcggaaaaga gaggatatcg agtcggccaa
      481 tcctccgaga ggactaattg cttgcagagc ctggtccggt gattcatgac cagcggtccc
      541 cgcaaattgg ccgtcacggc agtgggatca tctgaaggaa tggtcaagat cgtgacgacg
      601 gacagttcat cgctgtcctg cttttgaatt tcgatgagcg catccaacga cacctcgatc
      661 tgataatcgg gcttgaagaa gtcaggatcc aggatgacga aggccagctg gggttcttcg
      721 acacattgca gccacttgaa cggggcctcg gtatcgtgat cgagcagcac gtacttgctc
      781 cattccggaa agcccagaat accggaagga aacaccagga gcgtgtcgtc atttacctca
      841 aaagtcccga acctggtcga ctgacacttc atgacggttc ctcaacctcc acgacgcccg
      901 gttttgttcg acaattgacg aaacgcttcc aatggaataa cccctggccc ggcggcgatg
      961 cgattttcgt cttggatcct cgcatgcact tcatcgcgat gcacctgcac attgggcggt
     1021 gcttcgatcc cgagccggac ttggccgcct ttgatgccga gcaccacgat gcgaacatcg
     1081 ggaccgatgg tcacgccttc tccccggcgt cgtgttaaga ccagcataca agccttcctt
     1141 ggcgtgtgaa tacaagtaca tggatatatc ggtttcgatg aaggtgaact tgagcgtcga
     1201 tcagcggaga tagttgatca acgacgagtc gaaaattttg gtgaaggttt cactggcggc
     1261 ctgcacggcg acttcctgga ggcggagttg cgtaatggcc gtggcaaggt ccgcatcctg
     1321 attgtctgag atggattttg tgatggtcaa ggtggccgtg tccaaggcgt cgtgcgtgac
     1381 ctggaggcgg ttggcgagag cgccgacggt gccctgtaca tcgctgattt gagccgtcgc
     1441 caaatccaga tttcccagtc cggcttgaat accggagcga ttgttgcttt ccagcgccgt
     1501 gaggaggtcc cgcaacgaat cgaacatgtt ggtcgtcgat ccggtgaaga tgctgcttcc
     1561 cggcacgagg atctgcacgg tttgattttc accgaccgcg atggactggg tctcgctgtt
     1621 gccctgatag gcgaccgtat ctcctgatgt gatcgtgaag ggctgcacat ccgtcttcgt
     1681 accgccgaag atcgcctgcc ccgccacttc cgtgttgccg agttgaacca gctggcgctg
     1741 aagctgccgg acctctttgg cgatgctctg acgcccttct gcggacgtcg tgtcgctgct
     1801 ggcctgaatg gtcagctccc gcactctggt gatcaggttc tggacctgtc ccaaggcctg
     1861 atcggcggca ttcacacgtg aggtcccgaa attgatattg cgaacccact gcgtcgtctg
     1921 cgaaagcgcg gacttgtcga gcacgatctg tccgaatgcg ctcggatcgt ccgctggatt
     1981 ggtgacccgt ttttggctgg aaatttgttc ctgcgaggtg agcatctgca accgggagcg
     2041 ttgaagattg ccgagcagga ttccgtacat ttgttggtcg gcgactctca tactggctgc
     2101 tccctgtgag atgagaaggc ggctaccgct tcagggtcag aatcgtctgc agcatctcat
     2161 cgctggtggt aatgagtttc gaggctgcct caaatgcgcg ctgatacttc aacagattga
     2221 tgagctcttc atccatcgat actccggaca cttccgcccg gtgcgccagc agctggtcgt
     2281 gcaggatctc ctgcccttgc agatcgcgtg tggctccctg cagggtcgct ccgaagctgc
     2341 cggccatcgc gctgtagtat ccctgaaagg tcgtattgcc caatcccgcc accgaggccg
     2401 tttgcagatt gcttaacgcc agggcattca cattgttgcc gggcacgccg gcggccgtcg
     2461 atgaggccgc gatcttctgt cgatcggtca gggccaggct gatggtgccg gccgcggtcc
     2521 ctgaggccgt gaagaagtcc tgcgtcgtgg atccatccag accgtacccc aggcgatgct
     2581 gggtgttgac ctgcgagacg agttgagagg tgagggtatt gagcgatgtc tgcagaccgg
     2641 ctgcggtcgt gtcacgcgcg tcgatcaaac ccttgaggcg gccgccgctg atcgccgacg
     2701 tgatatccgt attgggtccg gttcccccat cgtagcgcac atccaggagc ccgttattgg
     2761 cgacgttcgc gacaccggtc agtgtgtagg ctgtcttctc agcgaccagc acctgcccaa
     2821 ttcccacgaa taccgtcacc tgtccggtcg cgtcctcaat cgaggagatc tcgaccaggc
     2881 cggcaaggtc gttgagaaag cgaccgcgtt gatcgcgcaa atcattggcg tgctggccgc
     2941 tgatctctgt cagcttgatc tggctgttga gatcggcaat cttgctcgcc agggcattga
     3001 cgtcgttaat gctgctctgc acctggccat cgagggaggt gcgttgagcc gacagttgtg
     3061 cgtcggcctg gttcaagagt ttggtcaatc catcggcttt tgtcagcagc accgtacggg
     3121 ccgtcagatc tgcaggattg gtggcgacat cctgccaggc cttgaagaat tcattgagtc
     3181 ccgccgcgat gccttggtcg ttcgcatcgt tgaagaccaa ttgaatttgc gagagcgctt
     3241 tctgtgaagc tccgaactgc cccacccgct cgttggaact taacaattga cgttccacaa
     3301 agctatcgac ggagcggcgg atttcagtgg ccgtgacgcc ggttccgatc tgacccggct
     3361 gaccattctc gggcaggctt tcgctgaggg tgatctcttg ccgggagtag cccggggtgc
     3421 tgacgttcgc gatattctgg cctgtgacgg acagggcgcg ctggaaggtg gccagcgcat
     3481 tggaaccgat gccgaataat ccattgagtc ccgacatacg accacccgtt atcccttggc
     3541 tctcactctg ccgccgtttc ctgcgaccgc ggcacccgag gacgaataga gtgccgccga
     3601 ctgcggtgag cgctgccaga tccggagcgt gccttgcaag aactcgagcg actgcccgat
     3661 gagggcggcg ttgaaccggt tcagccgatc cgtctgcccg atcgccgcga cgagatcagc
     3721 ctcctgttgc ttgagcgagg gcggcgagcc atacgaacca gacaaggttg cttcgttccg
     3781 gcgttgctgt tccaattcac gcatctcatc caatagttga gtttttcgca tggtcacgga
     3841 ggtgaattgt ccaaacgaaa gggagcgaat ggcgtcctgc tcctcaagca gcagggcctg
     3901 aaaggccgtg accttgtcga gcatgcggca aagaagggag tcggacgctg tggaaagtgg
     3961 ctgcggtacg gtcgacacca tgtctcccct tcaaacgaac ggttccgcca tgtgaggccg
     4021 cgtgatcgaa gagtcgagcc gtacgcgaag ggcgttacag gaccgcgtcg gtcaggacgt
     4081 tacgaatgat ggcatccgca accttcttac cgtccacgtt gtacgtgccc ccttcgaccg
     4141 actgacgaat ttgaccgact ctggcatcac gctccgcatc cggctgttcc gctgccgccc
     4201 tcagacgctg gagttccttg gcctgttcgg aaatctggac acgatcctgt gactgcgtcc
     4261 cttgagacgc ggtcttctta ttgtgtgtcc gttcagtgtc ctgaactccc aacaggattt
     4321 tcgcgagatc tgccgctcgg ccgttatgtg agatctccat gactgcttct cctcccccat
     4381 agccagggtc ttccgaaagg cctttcccct agtcgagtac tctatcggcg ggatcggccg
     4441 ggaactttag gaaaagcgat cgaattaggg aagtttttcc gcataggcat cgacgaatgc
     4501 cgtaatgccg atcccccctg cttctgtgga caatttggct atctcttgat cgtaaaagga
     4561 gtaccagacc tgctcgcttt tccggtccag gagtcccttc ggaacagttt ccctcatatt
     4621 tttaataaga tacgagatga aatacgcttc aaactcctgc ccggctttat ggagcgccgc
     4681 gcgggccttg tcccctgccg gcgcttgctc gaccggaccg cggaatccca gcggctgtgg
     4741 agtcgccatt tgggccagat ccaattggct ggacataaaa ttttgtattt ccattggaag
     4801 cgtggccctt tcttagacaa tctcaagatt cgcttgaagt gctccggcag aacgcagggc
     4861 cgataggatg gccacgagat ctcgcggagt cacgcccacc gcgttgagtg cccgtaccac
     4921 ttctccaagc gtcaccgttt gatcgaccac gatcagtcgc gactcctgtt cggtcacttc
     4981 cgtctgcaca tcgggagtga ccgtcgtttg cccttgggtc gagccgatca acggagccgg
     5041 aggctgtgac acgttgagcg tgttcttgac ggaaatcgtg aggttgccat gcgagatcgc
     5101 gcatgtggat aaccggacat gttcgccgag gacgaccgta ccggtgcgtt cattcaccac
     5161 cactttggcg gcgacatcga cggtcacgtc caatccttcc atcgtcgcga tgtattccac
     5221 gacccgtccg tgaaacgtcg cgggaagcga cgttttgacg aggcccgcat tcaccgggac
     5281 agccgtgccc ttcccaaacg tcccgtcgat cgcctccgcc gtgcgaatgg cggtggtgaa
     5341 atcgggatgc cgcagcagca cggacacgct gtcccagttc tcgatatcta ccaccaactc
     5401 cttttccacg atggcgccgg caggaaccac cccggccgct tgatggttct tggtgaccgt
     5461 cgcgccgccg gcgccgccgg tcccgccgag gaatccacca atggagaccg gcccctgagc
     5521 caccgcaaac acctgttggt tcggcgcctt cagcggcgtc gacagcagcg tgccgccttg
     5581 gagacttttc gcattggcca tggaagagac gaccgcgtcc agcgtcatgc cgggtttcac
     5641 aaacggcggg agcttcgccg tcaccatcac cgacgcgata ttgcgggtga gcaactggat
     5701 gggatcgatc accagattga tgcccatctt gttcaacatg gacatcatgg cttgaatcgt
     5761 gaactgtcca ccgatgacct gatcgcccgt ccggtcgaga ccgacgacca atccataccc
     5821 gatcagctgg ttttcgcgca cgccctcgat cgtggccacg tccttgactc gaacagcctg
     5881 ggctaatgag gccgtaaata acgtgatcgt gaggaaggcc agcatgccca ccacggtgcg
     5941 tcgaaaccag gccgttcctt gtggctgtct ttcaggaaca ggcgccggct tcagcggcgg
     6001 aggtgtccag gggcgcgtaa ggaggccggg atccattgag gccggtcgca gaaccccgct
     6061 tgaaagcatg agacgaaagc attgcgtaat catcgcccgt tgttgtttgc gcggcctcgt
     6121 catgttgact cctgtggctg gtcgattctt cccgacggac ttgcgattaa aacggataga
     6181 cccaatccag aattctgacg agccagccgg gccgctgcac atcgtcgacg actcctaggc
     6241 cggagtattc gatcttggca tcggcaatgg cactggactg caccgtgttc ttcgtattca
     6301 catccacgcg cctgacgatg cccccgatgg tcatgatctg gcgttccgaa ttgacggtca
     6361 cctcacgtcg gccctcgacc cgtaagtctc cgttcggcaa cacttccgtc acgatggccg
     6421 aaatcgtgcc cgtcaacgtg tcttcccggt tggttgcgcc tttgccgccg aacttgctct
     6481 tggcgctggc atcgataccg aaccctcgct tggcttctcc gcccaggcgg attcccggga
     6541 tgccgagata gcccatcccg gtgccgccga ttccattcga aatggtggag tcccgctctg
     6601 cgttcgtgtc ggcgctcttc gagcccttgt gtttttccac gattagaatc gtgatgatgt
     6661 cgccgatgcg catggcgcga agatcttcat agagataggc ccgcccattc tcttcctgcc
     6721 acagcgatcc cgtggttttc ggcggtggca atttggccat ctccaccttt tgttgcttcg
     6781 tgctgggcga ggccgaacaa ccgaccagca cgcacagcgc cagaccgcac cagatgaatc
     6841 cgatggggcg acattgcatg aaagagcggt taaaaactga cacggaccac ccccggggca
     6901 acgacggtcg cccgcaattc cttgccggaa tcgacattgg agaccgtgat ggtttgaccc
     6961 aattcaccat gcgatttggt gactccgacg gtttgaatcg acaacccgcc ctgccgggct
     7021 tcaatcgtga cccgatcgcc cttgtgcacg gcgaaagggc gccgcaccga ggtcatgcgt
     7081 atggcatttt gaggcggaag cgggcgaatg gccgctttac cgatgacctc ggcaggactg
     7141 gtcacaaacg gttgcttcag gtcgtacagg acgatccgtt ccgtggtgac gtcgtcggcc
     7201 gcaatttcct cgtcgacctt gatcgaccgc accggcacca ccacctcgac aatggcggcc
     7261 acatcggcgg tggcatcgac ggtcttgatg aatcgcccgt tcacggccag atgaatctgg
     7321 aacactcgac gtcccagcgg ttcgtccgat cgaccggcgc tgacttgcag atcgaccgtc
     7381 ccggagggta cgacgatcgg ctgctgagga tcgccgatcg ccacctggca gtcgacggcg
     7441 cgtccggcca gttcccgttt gacgaaatcg tggatcaccc cgcggatttg ttcgggatag
     7501 atgagccgtt tcccacgggc ctgcgggagg ccctgcgcct gatgcgcaac agccccctgc
     7561 acctggaaca gcggccggac cggagcccgc tcccctgcca tggccgggag gacggcgctc
     7621 gtgaccaggc tgatggtcgc aacagacagg aggctgagtc gattcacagg tgtcaccctt
     7681 ggttgaaggt gcggcgccga ttaccggcgc aggttgttgg caatctgcat catttcatcg
     7741 gatgcctgaa tggtcttgga gttaatttcg taactccgtt gggcgatgat catgttgacc
     7801 atctcctccg ccagattgac gttcgaactt tccaggaatc cctgctgcaa ggtgccgaag
     7861 ccggtcgaga atccgccggt accctgttgc gccggtccgg aggcaaagct gtccaggaac
     7921 aggttgccgc ccatcgccac caggcccgaa gggttgtcga agcgcaccag ctgaatctgc
     7981 cccacctgcg acgcttgtgt gacgccggga agcagaacgg agacggtgcc gtcctggccg
     8041 acatcgattt tcagcgcgcc cgacggaatc gtaatcaccg ggttgagctg gtcgccgtcg
     8101 ccggtcacga ggttgccgac gttatcgcgc ttgaaggatc cgttgcgggt gtacatgatg
     8161 gtcccgtcgg gccgggtcac ttggaaaaat ccggcgccgt cgatagccag gtcgagatcg
     8221 ttcccggtct gccgcatgtt cccttgcacc cattccttgg ccaccgtaat gggacgcaca
     8281 ccgcccccga cctgcacgcc gacaggaaag acgcccacgt tcgacgcgtt cgtgcccggt
     8341 aagcgttgaa tctgatacag cagatcgccg aattccgcac ggctgcgttt gaacgcgttc
     8401 gtgttcacgt tcgccagatt gttggcgatg gtatccacgt tcagttgctg ggccgtcatg
     8461 cccgtcgcgg ccgtccacat tgcgcgaatc atacgctcct cctattgcac ccggcccaga
     8521 tcctgaatgg ccgtttcggt catgcgatcc atcgtttgga tcaatttctg cgccgactcg
     8581 taactgcgca tgccttgcat catcttcacc atttcactca acgaattcac gttggactct
     8641 tcgatgtgtc ccgactgcac ctgcggcctt gtcgacaccg tcggtttccc tccgacaaac
     8701 agtccttcgg caaacttttg cggcatcgcg ttctccggga actcgaccac cttgatgctg
     8761 ccgatcggag tgccgtccac ctggatcgcc ccttccgcat tgatctgaat gttgccgggc
     8821 ggcaccttga tttccccttt ggtgcccatg acagggtgcc cgagattggt gaccaggcgt
     8881 cgttggctgt cgagagaaaa gatgccgttg cgcgtatagc gaaccccgtc cggggtcttg
     8941 acctcaaaaa acccgtcgtt ctgtatggcg acatccagtg gattaccggt aatgcggatc
     9001 cgccccggct caaaggccgt tcggacccca tgcacttcgg caaagacccg ttcgctggga
     9061 ccggccggtc tggtcgaaat ggcggaggag aggcctcctg acaccactcc catccccgtc
     9121 gaactggccc gggcgaatag gccgcggaat ccctgctggt cctgcttgaa tccggccgtg
     9181 ttcacgttcg ccagattatt ggcgaagacc gtgagttgtt tttcctgggc tacggcgcct
     9241 gagagtatgg ggtagatggc tcgattcata gtttgtactg tccagatgaa cctgttgtgc
     9301 tacaggggga gttagcaatg gacatgccat cgcaatacgt cagacggcct ggcacagccg
     9361 aagcgatcgc cggacgaaaa tccttgtgat tgtcgagaga tgggaaccgg aaggtatttg
     9421 aggggccgga gggaaattct tccgccgtca acggcgcctg tcaaacggcg tggcaaacgg
     9481 gaagtctttg ccggaagcag ggaaagtcct gcctacatag aatggggcgc aacgagaatt
     9541 ctggagatca cgcagcgatc gtggaggttg catcaacgga ggcggatacc atcgcaccgg
     9601 ggagtccata gagagcgtac ccttcggcac gagccgttgc gacggcccgg gtggccacat
     9661 cgaggagcgc gtcactgtcc atagtgtcct ggctggagag gctgattcct atgcggctgg
     9721 tgaggaacaa ttcgtgcccg tcgagggtga ccggcagcga tatcgtgagt tgcatcttcc
     9781 tggcgatggc cagcacctcc tccggacctg tgacctgatc cagcaggacg acaaaggtgc
     9841 gttcggcaat tcgcgctacc gtgtcggtgg tgcgcagggc gctcttcaca cgcgctgcgt
     9901 gaacgcgata catgaggttg aggttgatct gcgcctgttc gtcggttttc ggcgtgaagt
     9961 gatcgagttc gatcatcagg accgccacgg gccgcccata cttgtgagcg tgcactaagg
    10021 cttgattgag gagagatgta aagaggcgat aggtcggcaa tccggtgacc gagtcgtagg
    10081 tgttcatgcc ggacccgttc gattcttccg acagtgaatg catcctggaa gccgattgtg
    10141 ggccccgcgg agtccagagt gtcgcgccaa agaccatcag cgtgaggaga atgccggcgg
    10201 agatcatgac gggataggcc gtcaacgccg acagagtcag gaggcccacg gaggcagtcc
    10261 aatacaatcc gccggccatg aacaggatac aggccactac gccgagtcga gcgctctgaa
    10321 gcgattcggt acccgcttcg tcgtcgggcc acccatgccg gttcgttgaa tccgacccgt
    10381 tgcggtcgct cgaggtgttg agttcaggac gtgacgaatg cataccctcc ccaatggagc
    10441 atcgcagcca acgggatgtt gagctaggaa ggtatcggag aagtggaggc gaaactaaag
    10501 gcagtgtagc ccgcgcgacg atgcatgggc gccgccgaca cgaacgaaca gcaacgactg
    10561 ctacaccgtc cggaggggca cattacacat cgaccggttc gagccgcgct ttcaagtgga
    10621 ggatggcttt cgaatgaatc tggcatactc gtgattcggt caccttgagc gcctgcccga
    10681 tctccttcat cgtgagttcc tcgaaatagt agagcgacaa taccaaccgt tctttctccg
    10741 gcagatcctg gattgcagct tcgagcacgt ggcgtgcccg ttcgctcact aggaccgaga
    10801 gcggatccgg ctgattggag tcggtcaaca cactgatgat cttatgcccg tccgcatcct
    10861 gcacgccgag atcgtccagg ctgatcacca cggcgccacg tgagcgagtc aggaattcat
    10921 ccagttcctc cgtggacatc ttcagctctt tgccgacttc ctggtctgtc ggcgggcgtc
    10981 cgagtctgtg cagcagctcg gaataggtct tttgaaggag cgtaatccgc tcgtggaccg
    11041 agcgcgggat ccaatccatc gaccggattt catcgagcat ggcgccacgg atgcgaaact
    11101 ctgcgtaggt cttgaatttg gcttcgcggg tcggatcgta cttatccatg gcatccatga
    11161 gtcccatgat tccgacggac atcaaatctt ccgcgtccat ataggccggc aagcgaaacg
    11221 ccagtcgatg ggccatggcc ttcacgacgt gggtgaattc ctgaatcacc cgttcacgat
    11281 ccgcttccgc aatcacccga cgcggggcgt ttctgaccgc agacattttt cgagcgtcca
    11341 tcattcgcta cagtcctttc agtcagtgcg tccgcaaaag ttgttgccag aggaactgca
    11401 ctgtgccttt gggaagcgcc ggtggggtta actgagccac cgcctcagtc agttctcgaa
    11461 acgcacggct ggacggcgcg tgcggaaaga gatcgatgac ggcccgctgc tgggtcacgg
    11521 ccatcggcac atagtcgtcc aagggaatcg cgcccaggta atccagcgaa atatgcagga
    11581 atcgactgac ggccaactcc agtttccgga agatccgcgc ggcgtcccgc ggcgacttca
    11641 ccatgttgac gagcacatgg aagcggcgtt cccggtattg ccgcaacaag actttcatca
    11701 acgcataggc gtccgtcatg gaggtcggct cgggagacac cacgacgata atggattggg
    11761 ccgccgccgc aaagtaggtc acggtcgaag aaatgcccgc cccggtatcg atcaacaaca
    11821 catccatgtt cgatgccaag cgctcaagtt catcttgaag gatgagctgt tgcgcttccg
    11881 tcaggatcgt caactgagaa atgccggtac tggccgggag gacggtaatg ccatgcgggc
    11941 cggtcagcgc gatatcttcc agccggcagg tcttcgcgag cacgtgctcc aacgtatatt
    12001 tcggtgttag accgagcaga atatcgacgt ttcccaaccc gagatccgca tccagcacga
    12061 gcacacgctt tccggtttgg gccagggcga tggcggtatt cgccaccacg ttggtcttcc
    12121 ccaccccgcc ttttccgctg gtgacggtaa tcacttgcgt gcgggaggga ccgacgttgt
    12181 catcaagcgc tctcatctgc ggatccaaaa ttcctggttg catcgccatc gtcactcccc
    12241 tcttgttcgt cagtcgccta tcgcgcgtag gtcttgatac caggcacaac tgctgatgtc
    12301 gtctgccgat cccatgactg agtggtcccg cccgccggcg tccgtacctg cccccccagc
    12361 agcaggtcgc ccaggcgatc gacttgcgcc tgaaccaagt catcgggcac ccgttgaccg
    12421 gttccccaat aggacagcgg gatgccggtg cgatgagtgg cttcgaagat gccgccatag
    12481 cccgccgtct cgtcgagttt ggtaaacaac aaccggaggc ccggcacgtc tacgcattga
    12541 ctggtgctga ccacgagatc cggcactcgg gtgcctgccg cgaggacgag atggacctcc
    12601 atgggatagg ccccgttcag caaccctctc caacgttgcg ccgtgatggg ttcataggga
    12661 ttgaaccctg tggtgtcgat caaaatcagt tccgcttcac gggcctgcgc aatcgcggcg
    12721 cgggcctcct cacaggatgc tgccaccgag agctcgatac ccaggacttc ggcatacaag
    12781 cgaaggtgtt ccaccgccgc aggccgatac gtatccatgg taatcaaggc cacactccgc
    12841 tgctcgacaa tgccatagtg cgtcgccagt ttggcgatgg tcgaggtctt gccgactccg
    12901 ctggggccga cgaacagcgc aatcttttgt tcccctgccg cgctcagcaa cgggccggcc
    12961 gtgctgaccc gatccaacaa cactgtccgg agcaactgta gtgaggagtt ccgctgagcg
    13021 gacccctgct ccaccaacgt ttcacgcagt tcccgcagac aggcttccgc cgtggcatgc
    13081 tcgacgccct gagccaacag gtcttcatag acgcgctgga aggactggaa cgttgcgccc
    13141 tggttctccc cattgttgcg tgtggacgcc gatgtcgcgc gagtctcccg cgaaggacct
    13201 ggaacctgac gagccgccgg ccgcgactgc tccttccccg tttccatcgc tccctgcagg
    13261 gttttttgaa aacgcgattc ctccgccggc accatcggcg caggagtcac ggccgcacgt
    13321 gtctgctcat cttcaccgag cagcggcgcg gcctcgggaa cggcggcgtc atcttcaatg
    13381 gccgccatga cctccaatac cgacccgccc aacaacccga atccgttatc ccaggaacgc
    13441 acgcgtttcg tggacagaat caccgcatcc ggccctaacg tgtccttaat ggagcgaatg
    13501 gcctcatgca tcgacttgac gtggaatgtc cgtaccttca tgccaagctc ctggtttcac
    13561 cgacctcgag gtcgagccgt acggtttcca ctgcctgcaa acgtgtcacg ctttccacct
    13621 cgttcaatcc taaaatcgga accgagtgca aaattctgtc cgtcagtttc cgcagatgtc
    13681 gtcgcaacga aggcgagcac aaaatgaccg gttgatgccc gcgaccgacc atgcgttccg
    13741 cggcagcctt caatgcagtg agcagtttct gtgccaccag cggatcgggc acccactggg
    13801 ttccctgtcc ggctgcattc gcttgatcgg cgagggtacg atccaatcgc ggatccagtc
    13861 cgatgatcgg caacgagcca tctggcgcga gatattgctt cgtgatggtt cgtcccagcg
    13921 cttgcctggc gacttccgtc aaaatatcgg cgtcttttgt ggtcgccgca tggtcggcaa
    13981 tagtttcaag aatcgtccgc aagtcacgaa ttgggacacc ctctcgcagg agatgcgaca
    14041 aaactctgac gagcgtgccc aacgggatga gatttggaat cacttcctca accagcttgg
    14101 ggtgattttt gcccagttga tcgagcaagc cctgcacttc ttgccgcccc agcagttcat
    14161 gcgcatgccg cttgatcaac tcagacaagt gcgtcgcgat ggccgatccc ggatccacga
    14221 ccgtataccc cgccatttgc gcctgttcac gctgctgctc cggtacccac atggccggca
    14281 gaccgaacgc cggttctttg gtcggcagcc catggatagc tcctcgctga gccgtaccag
    14341 gatcgatcgc cagcacatgc gccggcatca cctccgactt ggcgagttcc acgcctttga
    14401 gcagcgccgc atattcgttg ggccgaagct gcaagttgtc gcgaatatgg atcggcggca
    14461 ggacaaagcc catttgttcg gcgaactgtc gccggagccc cttgatccgg tccagcaggg
    14521 cgccaccctg ccctccgtcc accaggctga tgagaccgta tccgacctgc acttccatga
    14581 gatccagggg gaccacctgg gtgccggcct cctcagcctt gacctgaggc gaggattgtg
    14641 tcggaacggc ttccgcggtc ttctgctgtt cgttgatctg atatgccatc caggcggtga
    14701 ggccgcccag cgcgaggaat gcgagatggg gaagtccggg gaccaggccc agcgtgatca
    14761 gaatccccga agccgtgccg atcgccttcg gggagatgag gacctggcgg ctgatctcaa
    14821 atcctaagtt cacctcggag gcggcgcgag tgatgacgat accggccgcg gttgaaacaa
    14881 tgagcgccgg cacttgcgcc acgaggcctt cacccaccgt caacaacgta taggtctgcg
    14941 ccgctgccgc cagcgtcatg ccctgttgca acacaccgat cgtcaacccg ccaagaatgt
    15001 tgacgagcgt gatgatgacg gccgccacgg cgtcgccgcg cacaaatttg ctcgaaccgt
    15061 ccatggcgcc gtagaaatcc gcttcctggg cgatctcctt ccgccgttgg cgggcctctt
    15121 tgtcattgat caagccggcg ttcagatcgg cgtcgatgct catctgtttg ccgggcatcg
    15181 cgtccaaggt gaaccgggct gccacttcgg cgacgcgccc ggcgcccttc gtgatgacca
    15241 cgaagttgat gatgacgaga atggtgaaca ccacgagccc gacggtatag ttgccgccga
    15301 ccacgaacgt gccgaaggcc cggatgacct cccctgcagc ggcggcgcct tcattgccgt
    15361 gcaggagaat caagcgcgtg gaggcgatat tgagggcgag acgcaggagt gtcaccatca
    15421 gcagaatcga ggggaacacc gagaattcca acggacggcg tacctgcatg ccgaccagca
    15481 gaatgatgat ggacagcgtg atgttgaagg ccaggagcag atccagaaaa aaccggggaa
    15541 gaggaatcaa catgaccatc aaaatgccca caacccccac ggacatcatg atgtcggggt
    15601 gtttgaccaa ggaggtcgtg gaaaccgatg tgttctcgtt cgccatctag actccaatcc
    15661 aatcggatct acgccggcgg cagtttgccg cgcacgcgat acacaaacgc caggatttcg
    15721 gcgactgcac gatagagatc ctcgggcact tcccggccca cctcgaccag tttgaagagg
    15781 gttcgtgcga ccagcttgtt ttcgacgatc atgactccgt gttgtcgacc gatttcccgg
    15841 atcttttccg cgacaaagcc ggcgcctttt gcgacgacga ccggcgctcc catcgccttg
    15901 gagtcatatt tcagggccac cgccaggtgt gtcgggttgg tgatgatcac gtcggccttg
    15961 ggtacctctg ccatcatgcg tttccgggac atctctctct gagtgctgcg gatcttggcc
    16021 ttcagccctg ggtctccttc cgccgcccga ctttcctctt tgatttcgtc gcgtgacatg
    16081 cggaggtcac gctcccattc aaaccgttgg aagccatagt cgaggccgcc gatcaccagg
    16141 gctgcaccac ccatcatgag cgcagctttg aatgtcgccc accctaccgt cggcaagagc
    16201 gtctccatgc cgaattgtgt cagaggagag aacaacagca tatcctgctt gatcgtgaga
    16261 tagccgactc ctccgatggc gaagatcttg agccacgatt tcaccagttc gctgaaggac
    16321 cggacggaga agagccgtga gaaaccgccc atcgggctaa tgcgggaaaa ctccaactgc
    16381 agtccgtctc gcttccagag gaaaccggtt tgcatgaggt tggcacccac gccgaccacc
    16441 gcgatcccgg caatgaccgg gccgagcatg ataaacacat cgatcccgat ctgccttaag
    16501 atcaagtgca gatggtcgag actcagtgtc cgctgtgtgg actcctccat ggatttgctc
    16561 agccacaact gcagcgactc tcgaagcttg ttcaggatgc ccggcgtcat ccaatagagg
    16621 gcgcccaagc tccccagcaa tcctactgcc atggcggcat cccggctgag agcgacctgg
    16681 cctttcgccc tcgcatccgc tttgcgtttc ggggttgcct gttctgtccg attctgtccg
    16741 ctgtcagcca tgaccgagtg ccttcaggag tccgagaatg gtttccacca gggtttcgaa
    16801 ttctttttca aacaacaaca aggtgaaggg catcgccagg ctcatcgcga ggaagcccgc
    16861 cccgatggtg atcggaaaac tcgtgacgaa cacgttcatc tgaggtaccg cccgaccaag
    16921 gattgccagg acggtattga cgatcaggag agtcgcgaag acgggagccg cgagcttgag
    16981 ggcgaccccc agcatgttct gcgccaggtg aacgatatcc tgagcgatgc cgtcggataa
    17041 cgaagcgccg aacggcggca cgtattcgta gctcatgccg atcgcatgca ccaccagcag
    17101 atgcgcgttg agagagagga ataccaagga gccgagcgtg agttgaaatt gtgcaatgat
    17161 cggcgtgttc tggtggctca tgggatcgaa catttgaatg gcgctgaagc ccatctgcgt
    17221 gccgatcatg tcaccggcga cctggaagcc cgagaacagc agtcgcactg ccaaaccaat
    17281 cgtcagtccg atcagaaact cactgccgat cccggcgatg accaaggccg gatccgtcgg
    17341 caggctcggc aggtgaatga tcggcgccaa gaccaggccc agcgtggcga cgagtcctgt
    17401 tttgaccggc attggaatcg tgcgcgtatt gaggatagga aagacgctga caatgccggc
    17461 aatccgcacc agcaggatgg aaaaggcctg gaactgaggc agtgcgagat gcagggtttg
    17521 cgcagcgctc atcgtagtaa cgggcctatc agtgaatcag ggacgggata ctcgtgatga
    17581 tatgggtcat aaatgcaatg aggacaccca tcatccacgg cagaaacaag agtgtggcgg
    17641 cgaacaccgc cagcacctta ggcacaaagg tcaacgtcgc ttcgttgatc tgagtcatcg
    17701 cctgaaaggt gctgaccagc aaccctacga tcaggctcaa acccaagatc ggcgccgaca
    17761 ccagcatggt ggtttcaatc gcttggcgac cgatttctgt gactgtttca atcgtcatgc
    17821 gtgcgctcct actgaaagct cctgaccatc gatccgacga ccagatacca gccgtcagcc
    17881 agcacgaaca aaatcaattt gaacggcaag gagatcatga cgggcggcaa caacatcatg
    17941 cccatagaca tcaggacact ggcgacgacc atgtccacga tgaggaacgg aatgtagagg
    18001 agaaatccga tttgaaacga aatgcgcagc tcactcagga cgaaggcagg aatgatcacg
    18061 tggatcggca cctgttctac ttgcgtcgct ttgggcgcat gcgacagctc catgaacagt
    18121 tcgagatcct tgtcgcgcat ttgtttcaac atgaacgccc ggatcggctg aatccctcga
    18181 ttccaggcat cttcgtaccc gatttgctcg gcgagcagcg gttgcagggc atccttgtag
    18241 acggcctgtc cgacgggagc catcacgaac atggtcagaa acaacgcgag gctgatcaga
    18301 acttgattgg gcggtacctg ctgcgtcccc atggcctggc gaagaaagga cagcacgatg
    18361 accatacgcg taaacgacgt caccatgatg aacaaggcgg gcgctaggga gaggacggtg
    18421 aacagggcca gaatctgcag gaccacggca gtctgcttgc tgccgccctg gccgagatcg
    18481 atcgtcaagg aaggcccttc ggcgtatgcg aaggagacgc tgaacaggca ggcgagcacg
    18541 accagactga tgaggagacc ggcccaccat ccggaacgtt gcgcggccga cgcctctctc
    18601 ggcgggctca ggagactagc tgtgtgcacg tgctgtctcc tcaaggttgc cggacccggg
    18661 gattccgatt gctgctccgg acggcagcac ggacgcctcg gcgatgaggc gtttcgcgac
    18721 gtcaggatcg gcaatttttc cgagcggcac caaatcggtg gcggtggtgc ccaggatgag
    18781 cacctctcct gccactgcca ccaccatcac gtgctttctg gatccgagcg gaccacttcc
    18841 taaaatcttg accaacggtg cgccattcac agggagaaac cgacgcccga cggcggatcg
    18901 cgccacggcc agcagaccca ggatcaatgc caggacgacc ccgagcgccg acaccatgcg
    18961 aaccacgctt tcccaaatct ccatggggac cgttcattcc tctcgccgcc ggagcggcgg
    19021 cattgccgca cgttgcgccg actacccgag ttgctgaatg cgctgattcg gactcacgac
    19081 atccgtgagg cgaatcccga acttttcgtt gaccacgacg acttcgccgc gcgcgaccaa
    19141 tttgttgttg accagcactt ccatcggttc gccggccagc ttgttgagtt cgatgaccga
    19201 cccctgcccc aactgcagca actcccggat cagcatccgt gtcgagccga tctgcacggt
    19261 cacttgcagc ggaatatcga gcacaaactc gatgttcttg ttggcgggca cggcgttctg
    19321 attgtcaaca gggggaaacg aggtcggttg cacgttgatg tccccggcgc cggcattgga
    19381 agcgtccgac ggtgcagtgg tgtctccgtt gcccatgcgc aaatctcctt tagttccggg
    19441 acgctctacc ccaatatctt ggtcacgcgg cacgcatgat tacccttaaa gatcccgggc
    19501 tgcccttgaa atttcggatc gccctcgacc aggcacagca tcggatcgcc gggatgttgg
    19561 tcgagcatca acacatcgcc gggattgaaa ttgagcaagt cctgcacatt gatacgcgcg
    19621 gttcccaact gcacggtcat agccacgtcg cattcctgta aggaatcctt gaaacgactg
    19681 ccccaactgc tgtcctgctc gacattgtcg gcaaacaaac cggaatacag tttttctttg
    19741 atcggctcca acatggagta gggatatgcg ataaacattt cgcgggagat gtcgcccagg
    19801 tgcacctgta acgtcaccac cacgacgatt tcagacgtcg tcacgaccat ggcgaactgc
    19861 gggttgctct ccgatcgcaa gtagccgatc ttcaccggca tcacggcctg ccaggctttc
    19921 tccagatcgg ccagcgcctg ctgaaccagc ttcttaatca gccgaacttg aatggtggtg
    19981 aactctctgc cttcaggctt cacatgcgtt tgcacttttc caccgaagaa gaaatccaca
    20041 atcaggtaca ccagcatggc atccatgacg tagagcccgt tgcctcgcat cggctccatg
    20101 gcgaacagat tgaaggagga gggaaccgga actttttgaa tgaagtcccc gaacttcatg
    20161 atttgggtgc cgaggacgct gaattcgatc ttctcgcgca gcacggcact ccaggagatg
    20221 gattgctgac gcgtgaaccg atcgttgatc atctccatcg tgggcatacg cccgcgaatg
    20281 atgcgttcct ggctggtgag gctgtacgtt ttgaccccgc ccggatcttc ctcttccttc
    20341 ggagccgtct ccacttcgcc ggacatgacg ccgcccatta aggcatcgat ttcttcttga
    20401 gagagtattt tatccatggc gtcccggcga acgacgatcg actattgaat gacgaattcc
    20461 gtgaagtagg tggacttcac cgactttttc ggcagcaacc cgttgatgcg atccgtgatg
    20521 tcctcgcgca atttgtgttt accttgaggc gagcgggcgg tggcggaatc caacccggtc
    20581 agcaacacga ggatggcgtc gcggatttgc ggagtgcgcg ctttgatctg ctcaacggtg
    20641 tttgagctgt cggcctccac cttgagggtc actttgaggt aacgaatttc aggcgtgtcc
    20701 gcaaggttga cgataaaggg gtccaggtcg gcaatcgcac tggcatcggc aacggcccca
    20761 tgccccccgc ctttgtcttc ggcctccccg tgagcgactt gcttctctcc tccgttttcg
    20821 ctatgcgcct tctcgccgga gggtccgccc atcattttga acatcgcaaa ggcgccaccc
    20881 aggccgagaa ccaatacccc cgcgacgatg atgatgacca tcttcatggg aatacccgcg
    20941 ggcgctgcgg gcgccttctc ttcagttgct tctgacatac gaacctcctg cggacagtgt
    21001 ttgccggcgc tgctcggttg tgcaatgcat atgccacgcg acgaccgaca acaggcctct
    21061 acagatttcg ctctctggac cggcgcctgc gataacccac tgatttcagg tcacgacgtt
    21121 tcccgggcac gtgcgaacga agaccgggcc tgagaccgtg tcagcggagt gacgcgggct
    21181 ttgcgagggc cgacagaatg ttgacgacgg tgagaagacg tgaggggact gtgtgttggc
    21241 gaaacgctag gtagaaattg ccgacttggg ccctgacgcg tgcttaccag gcgcatcagg
    21301 gccctcctcg cttaccgttt gagattcacg agttcctgca acatttcgtc gctcgtcgta
    21361 atcgcacgtg aattggcttg aaatgcgcgc tgcgtaataa tcatgtccac aaactccttc
    21421 ccgagatcca cgttggattg ctccacggtt cccgacagga ctctgcccaa tccatttacg
    21481 gtcggggcac cggccagagc cgccccggac tcgaccgtct ccagaaagtg attgtcgccg
    21541 acgttgacca agccgtcggg attggtaaag cggctcaaga cgacctgagc aagagcacgt
    21601 gtttccccgt tcgtgaattg ggccatcact cgtccttctt tatcgacgct gatgcggtcg
    21661 agcgtgcccg atgaatagcc gtcttgcgtt tgactcgcga caccggaagc ggcgccgaat
    21721 tgcgtcagag aggacaaatc caccgtcatg gcctgcggcg tggtggcgcc gttggtcagt
    21781 gtcgcagtca ccgtctgtgt tccgcccgcg gtcacggcgc cgctggcgtt aaacgtcatg
    21841 ttggtttggg cggcagccgc acccccgtcg atttgagagt acacgcccca ggcattcgcc
    21901 gccgtcttgc ggaaatacaa ctgcatttga tgggagttcc ccagggagtc atagaacgtc
    21961 atgcccgtgg agaaattgta ggaggtcgta tcggtgacgc tgaaggctgt cgtcggcgcc
    22021 gtcgcattgg cattgagatt cccaagtatc tcggcgctgg tcgtcgcgac cggcggtacg
    22081 gcactcgatg tcagtgtaat gccgccgatc gtgctcgtga tgttgccgtt cgtgtcggcc
    22141 tggtagcctt gcaacagagc tccgctgcta tcgacgacct gaccgacact gtcgactttg
    22201 aattggccgg cccgcgaata gaaattggcg ccggacgtgt ttctcaccag gtagaagccg
    22261 tttccatcga tcgccaggtc gaaggtattg cctgtggtcg tcatcgaccc ttgggagaaa
    22321 ttggtctgca catcgttgag gaacacgccg agaccgattt gatcgctgcc ggagccaccg
    22381 cccaggctgg atgaaatcag gtccgaaaag gtcgcccggc tggacttaaa tcctgcggtc
    22441 ccgacgttgg cgatattgtt cccgatcacc cccatggcgt tgccgtagga gctcaagccg
    22501 gtcactgccg taaacatcga cgttaaaatg cccattgtct catcctcctt gttaccacga
    22561 cctgaaacgt tctcccaagc gttcgcccgc accgcagggc gatggccgtt atgcgacggc
    22621 cgtcgtgccg gtggtcgcgg ttttgttctg cgcgttttcc tgaagcgtca cacttttctc
    22681 catcaaggac accagcttgg acatttgttc cagctgcgag aactgtgcgg tttgcgcgac
    22741 gaagtcctca ttttccatcg gcttcagcgg atcttgatgc tgaagctggg tgacgagcag
    22801 cttcaggaaa tcgtcttttc ccagcggctt cggaccatcg gtggtcggcg tggatgagtc
    22861 tgggggtgtc gcctgtgtaa tcaccttgct gatatccatg ggacctccaa tcatgcaaat
    22921 atactgacgc gttcattcgt gtagtcgccg gtctctcccc tcgcactgcg ctcttcttct
    22981 cccgtcatgg accgctggcc ggacgaggtc gtcgggcgat cctgatgctg ctgttgaaac
    23041 atccaggcat tgtcgttccg accctgttgt tgatcgacgg tgacccgcaa catgcccatt
    23101 tcgaggcctg tcgtgcggag cgatgattcc agtgactgcc cctgttgcag gaggccttgt
    23161 cccagttctc catgttccgt ccggatatgc gcatggaccg tttggtcgct catcatgaca
    23221 cggactcgca aggggcccat gtccagggga tccaagtcca gcatgacgct ttgtgagacg
    23281 ggcgaggctc ctcgaagctc gctcatgcgt tcggcttctg ctgctcgcag gactgcagcg
    23341 ggctggccgg cctcgttgcg gccgacggcg ctttcggagg gaggcggggc cggttgattc
    23401 agtccactca tccggtccag gaacaacgaa cgatcgatcg gcccgttgct catggcttta
    23461 ttcggcatcg agcgatcggc atcggcagaa gagtcggaaa acggctctga ctcattctcg
    23521 agaaatttcc cggcgttcgc acttccctgt tcggtgctca ccggactgtc ctgtgcgaga
    23581 tcttcaagaa atgccggccg gtcaacccga tcgccgtcag cctgtttcat gcctcctgcc
    23641 tctgccgtgc gtgtcgtgac agccgcagcg acagtcgtca ctttctccgg atcgatcgtg
    23701 ctcgcggtga cggccgcgtc cgttcgatcc gcgctcactt ctctcgcctc accatgactg
    23761 tcgggatgag ggaacgccga catatcaatc attccctcgc ctggctgttc tttttcagcg
    23821 gattctttta tgtctgacgt ctgcgcctgg gccgttgcag cctggccgac gagtgcctct
    23881 tcagatgtct gtaccgaggg atccagcgct gcgagctgag cggaaggcgt ctcgtgaggg
    23941 cctgtggcgg ctgtaggcgc cacttttccg gcgccatccg cagaggggtc caattccgaa
    24001 tccgtttcac cactgatctg tactggcgtc tgtgcgaccg caggcgtggg aagaagcaac
    24061 gcggccacaa ggatctcctg ccgttgcgtg ctctgatcct cagcctgttc agtattgtct
    24121 tcagcattgg ggctctcgac ttccgtgtcg gacacacagt cagccttcac acaggactct
    24181 tcttttaggc tggcagctcg ttcctgccgt gcgaactccc tagagcgagc agctccttgc
    24241 ttgtctgatt ccaccttgga atgatgcgac gaacgagtcg gtgaagccgg tttcgatgcg
    24301 gctcgagctg aggccggttc cggtgtcatc gccccccggt gggcttctgc tcgctcaagc
    24361 aacgaatcga atgtgcgaga tggcgccgaa ccgtgagccg tcgcctggcg ttctcgcgcg
    24421 actggggcgc catgagtgct gtgacttgga attccgttca agaagggctg catgtcagtg
    24481 gccataggtg ttcctgtccg tttcgaaccc cgtgcgttgt catggaacag cattacaaag
    24541 gccgtgccat ccacgtcgaa cgagaaaagc gcgcatgaca cgggattttt gcaaggagga
    24601 ctaaacgcgg agacttgaag acggaagttt ctgcaggagg tgacggcaaa acctgccgaa
    24661 tcaccgggac acgaagggtc tgtttacggg gccgtcagca attgttcggt caggcgggca
    24721 gcgcgctccg gtttgacttc agcgaggatc gccccggcag atttgccctt aatcaggcgt
    24781 aacacttcga gcgccttcct atccggcata cgctccagac gggcggcggc ttcctcagcc
    24841 ggcatcgctt cgtagatttt cgccagctgg gtctgattga cgttggccgc ccctcccggt
    24901 ttggccgtcg gcttgggctt ctccgcttca ctcgtcgcct tgttctgggc tgcctgacga
    24961 cgcttttttt cagcctcaac tgcctgttca tgccgcgtca caatctgttc gagttccgct
    25021 ttgagttcca ggagatgcat ctccgatccg cgtaacgccg cctcacgctt atccagggcc
    25081 cgtttgcgct gctccagcat ctgaaaaatt tcacgcggcg tgctgatggc aggaccttgg
    25141 acctcttttt tgatcgcgtc ggcttttggt tcttccttcg ctgctgccgg agccggcgcc
    25201 ggagcacctt catccgcgac gactgccgtg cccatgcctc ccagaagggc cacactgaag
    25261 gcaaccctga ggatccccct tgtgcagatt gttcgaacgt cacccctcgc catcgcgatc
    25321 atgcccgttc tccctgttgt aatggactcc gtcgccaggc tcgttcatcc agcagctgct
    25381 gatcctgccg ttctcgtcga cgacgtcgtt ccagcgcggc gcgcgcatca aggaggtcca
    25441 gcttcttgcg atactgcatg gcttccagga gctctccgcg tttgtcttcc attttgacct
    25501 gttgatccct ctgcatctgc tccatgctct tccgtgctgc gatggcctga tccatcgcgt
    25561 cgaggtgacc gtacacctga tccacggtgc tcccctgtcg cacctggagc aaatattggt
    25621 cggcatccgc cctcgcccgc gaatccaaca tcgtcatacg gtcgaccgtc tgctgcagcg
    25681 cgctcgcgag ttccgccaac tcgagcttgg cgacctcttc aagttgaatc gcgtaggtgc
    25741 ggagcacggt cacgttcatt gaatcgttcc cgccagcgct agcaaggcat cgatggactg
    25801 cgacaacccg acggactgct tcgtctcctg ccgcagaaac gccgtgatgc cttcatgagc
    25861 ggacacggct ttatccagct ccttgctcgt gcccggctta tacgcgccca gtgtgatgag
    25921 atcttcagat cgccgataga gcgccgtgcg ttccaggacc aatcgtgccg cggcatagtg
    25981 tgccggcgtg acaatgtcgc gcatgacccg gctggtactc tgcagaatat cgatggccgg
    26041 aaagtgattg cgcgcggcga gttcacgcga aagcacaatg tgtccgtcga gaatggagcg
    26101 tacggcatcc gcgaccggat cattgaggtc gtcgccatcg accagcacgg tatagagccc
    26161 ggtaatgctg cccccgcttt gtgccggtcc gacccgttcc aacaacttcg gcaacacggc
    26221 aaagacggac ggtgtatacc ctttggtggt gggaggttca ccgatggcga gcccgacttc
    26281 cctctggcta taggccaggc gggaaaggga atccatcatc agcaacacat gtttgccgca
    26341 atcgcgaaaa tattcggcaa tagccgtcgc cactaaggcg ccgcgaatgc gcaccagcgg
    26401 cggttgatcc gaagtcgcga ctacaacgat ggagcgggcc cgtgcttccg gtgtcaggtc
    26461 gcgctccagg aactctttga cctcgcgtcc ccgttcgccg acgagggcga tgaccctgac
    26521 gtccgcctgc gtataccggc tgatcatgcc caacaagacg ctttttccca ccccggagcc
    26581 ggcgaacacg ccgattttct ggccttggcc gcaggtcaac agggcattga tcgcccgaat
    26641 gccaagatcg accggctggg taattctggc gcgatgcaat ggattcagcg gaggcgcata
    26701 caatggaacc tggacgtcgg tgcgcaccgg cggcaatccg tccagcgggt gcccgagtcc
    26761 atcgaaaatg cgccccaaca tctgcggtcc gaccgacacc gaagccacgt gccctttcat
    26821 cacgacacgg ctgcccggtc cgatgccctg catttcaccc agcggcatca acagcacacg
    26881 atcctcccgg aatccgacca cttctgccat cacggcgcca tgaccacctt cgcgagtgat
    26941 ctcgcagacc tctccgacag aggtgaacgg ccccgaggcc tcaatgacga tcccgacggc
    27001 ttgggccacc cggcccgtga cggacaaggg ttcgacttgt tcgagacgtt cgctaagctt
    27061 caccggtatc ccgctttctc aaggcttcac cgagccgcag cagttgggta tccagcgtgg
    27121 cgtcgatcaa gaggctctgc gcctgcacga gacagccccc cgggctaata ccgggatccg
    27181 tttcaaattt cagggtcaat agcccatgcg gagtctgact caatgccgcc cgcgacgctt
    27241 cgagcagcgg cagatccgac ggatgcaccc ggatccggac aagcccgctt tcatgcaaat
    27301 gggccagtgc cgttcgtacc tgcgtcacga tcacctcacg tttttccgtc accagttcgc
    27361 gcaggacctt ttgcgcgatc atgaacgaaa gggatgcgac ttcttcttcc accgtgtcgt
    27421 gcagattgcg ccagcttccc tccaactgcg ccatcagtga ggcgaccagc gcctgttccc
    27481 gttgacgcag cgaggagacc cgctccgtgg cccgcttttc cccctccgcc aagccccgct
    27541 cgaattcggt cacgtcatcc gttccgaagg gccgattccc tcggctgaaa tcggccagcg
    27601 gcaccgtccg caactgaatg ttgccggaac gaatcaaggt gcgcgtgagg accgtattct
    27661 cctgcctgag acgatagagc tccagcagtc gcgacacgga ttgtctgacc aattccactt
    27721 cgaacggttt ggttaagaag tccgcggcgc ctgatttcat ggcacgcaca gccagttcga
    27781 tgttgccgtg tccggtcata acgatactca ggagacggct atcgacctcc aacgcctgtt
    27841 gcaagaaagc aattccatcc gttccggcca attgaacatc gacgatcagc acggcaatcg
    27901 ggcgggactt gaggtgcgtc agggcctcgc tgacagaccc ggccgtgaga atcgggtgcc
    27961 ctgtggactg cagcatttcg gtcaagagga agccgatgga tggttcgtcg tcaacgatga
    28021 gaatggcccg tggggcgagt tccttctcat tgtccgcggg agacatctgt ggcaggcgac
    28081 ttcccacatc aacggttctc atctacagca actcctcgcc tcccccgccc ccgatcacga
    28141 tgcgaccttc ctcctcaagc ttgcgacaga ctttgagaat gttctgctgc gctttctcga
    28201 catcggatac cttgaccggc ccgcgcgttt ccatgtcttc cttgagcatt tcggcggcgc
    28261 gactggacat attcttgaga aacttctcct tgatctccgg cgtggcgcca cgaagcgcga
    28321 ccgggaggtc ttctttgctg atttccttca tgagttcctg catgccgcgg gaatccagtt
    28381 ccaccaggtc atcgaagacg aacatcagcg cccggatgct ttctgccagg gcctgatcgc
    28441 gttcggtgat cttggccata atggcccctt cggtgttctt atccacacgc gtcagaatat
    28501 cggccatcag ctcggcgccg ccgaccgaca tgctcgacat gcccatcgaa gccgacagaa
    28561 tttcctgcaa actatcgctc aattcggaca gcacttccgg ctgcacttct tgcattgtcg
    28621 ccaatcgcag cgacacgtcg gcatgcagat gtaccggcaa caacgccagc acggcactgg
    28681 cctgttcggc ttccatttgt cccaggcaga cggcgatcgt ctggggatgt tcgatcttga
    28741 gaatttgcgc gacggttcgg ggatcgaccc acttcagggc gtcgatacct ggataggtct
    28801 tcgtattcaa ggactccatc attctggcag ccttttcagg acccagcgcc ttcttcagaa
    28861 tggcttccat gaattcgcgt ccctcgaatt gcacctcgcc ggatgagctc gcctgctcaa
    28921 attcggcgat cacgctgtcc tcttcctgtt tcgtaatgtt tgcggtctct ttcatgaagc
    28981 tcccgagctt gcgaatatcc ttcgggtcga gggtcttcat caccgccgcc gccgcttcct
    29041 caccgatggc tcgaagtagg atcgccgctt tttgtgcgcc cgacagttct ttattcatga
    29101 tctgcttggt taggcgttac tcttcaacca ctgtttcacg acgacggcgg tattggcggg
    29161 gttgttcttg gccatatcga ggatctgccc cggctgcttg ccgctgatgg ccgcttcaac
    29221 ctgtcccacc gacgcgggca gcgccggcgt ctcgccgccg ccggctgcgg gcgcggactg
    29281 aaccagcatc accatcaacg ggcgaaccac catgaagaaa atcaggaaaa acagaacacc
    29341 gccgaccgcg tagcgcacgt aaggcatcca ggccttgctc gcatccggcg tagcctcgac
    29401 cccaccggcc ggttcctccg ctcctagccc gaactgaatg ctcaccactt ccacttgatc
    29461 ctgccgttcg gtcgaatacc ccatcgcctt cttgacgatt tcttcgatgc gtttcatttc
    29521 ttcctcagaa cgaggcacat atttcttggg ctgatccgca ccgccttcgc ccgccttgcc
    29581 cccgccctca tacgtgccgt ctaccaggac tgcgaccgag agcttcttga tggtgccggt
    29641 cggctccacg atgcgcgaca cggtccggct gatttcataa ttgaccgtct catttttcgt
    29701 ctggttgttg ttcgagctgg tttgtccgcc ttcggcttcg gtgccacccg gcacgtttga
    29761 ctcgacgccg ggcacgccgc ccgaggtgcc gttcaccccg ctcgattttt cctgtccgcg
    29821 ctgctcactg cgaacgacct gcccgttcgg atcgtagcgt tcttcggtgg tttcgatttt
    29881 gcggaaatcc agcacactcg acacgcgcac cacggcctta ttgacgccga caatccgttc
    29941 caacatcgtt tgaatacggg tctcgatgtc cttttccagc gtacgctgat attccatctg
    30001 cgttccggag agcccggctg attcatcgct ggaggtgttg gacaataaat tgccgtggcc
    30061 atcgacaacg gtcacatcac gagcttgaag cccttcgacg ctgctggaca ccaggtgcac
    30121 cacgccctga atttgcgcct tcgacaacgt ttgactggcg cgcagggaca ccaccacaga
    30181 ggctcgcgca cggtcctgct cggtggcgaa cagccgtcgt tcaggaatcg ccaaatgcac
    30241 ccgtgctcgt tccacctccg gcatttgagt gatcgtgcgg gccaactcgc cctgcaaggc
    30301 ccgtcgatag ttcaacttct gcacgaaatc ggacatgccc atggtcgtcc gatcgaaaat
    30361 ctcatatccc acgcccccgc catgcgggag accttgcccc gccatctcca aacgcaagtc
    30421 atgcacctgc gcgctgggca ccagcacggt ggtccctccg ttggccgtct cgtagggcac
    30481 cttcgcatct ttcagtttgt cgatgatgcc tgacgcatca tcgaccgcca ggttggcaaa
    30541 gagcacctgc atatccggct gttgggtcca gagggttacc gcgaccagtc ctgcaatgga
    30601 cccggcgagc gcgagcagga tgatgaagcg ttggttgatc gtgaactggc tgaattttga
    30661 aaacatacgg tgtggaatcc tttatgcgtg agaccggcga ccggacaaca accgcctaaa
    30721 tctgcatccg ctgaatttcc tcgtaggcgg tgaccagctt gttccgcacc tgcatcatca
    30781 actgaaaaga cacatcggcc tgttgcaatg ccaccatggt ttgatgaatg ttggtgctct
    30841 gcccggtcac caacgcatcg acggcctggc tggcgccggt ctgtgcatcg ttgatatgcc
    30901 cgatagcctc cttgagagag cccatgaagt tcgctgcgcc ggcctcgccg cctggtcccg
    30961 cctcgtgaat ctcaggcatc tcaatcggtc ggaagccatt cgctcccgca atacgcagat
    31021 cagacatgac tacctcccga tctccaacgc acgattccac atggtgcggg tcgcgttaat
    31081 cgcctgcacg ttcgcttcat aggcgcgcga ggcgccgatc atgttgacca tctcttccat
    31141 gacattcaca ttgggcatcg tcacgaatcc ctttttatcc gcatcgggat gacgcgggtc
    31201 atacaccgtc tgtccgggct tcttgtcttc gatgacgcga gcgactttga caccgtccaa
    31261 cgcttgacgg cctggtccgg acgtcacttg cttgaatgcc ttttgaaacg ccgaggatac
    31321 gggcgcggcc tgaaacacga catcacggcg gcgatagggg ccgcccgtgc tggttttcgt
    31381 cgactgggcg ttggccaggt tgctggcaat gacattgagt cgatgccgat gggcatccag
    31441 cgcggagacg gagaccgcaa ggctatctgt caattccatc acacacctct ttgtagttgc
    31501 ttccggaagt catgagttga gtgtttccca cgatcgcgcc taccgtcctt ctcgaatggc
    31561 acccaaaaga cctttgaact tcatcccgat gatggtcgct gcggtgttgt attgctgagc
    31621 gttatccgac attttggcca tttccaattc aatgttgacg gaattggcgt ccagcggcag
    31681 atcgcctgcc ggcacgtctt cgagctggcc gtggacccgt tgataaccag tgccttgtgg
    31741 cccgaaatgt tgagcgttgg tggaaaccag agagatcggc aacttgccct gctgtgcact
    31801 ggccagtgcc tgaccgaaat tcaaatcctt cgcgcgatac ttgggcgttt cttcgttggc
    31861 gatattcgcc gcgatcacct gctggcgagc accgcgaaga tccagcgacc gactcaacaa
    31921 ctgcatggtt ctgtcaaaaa tagtcatgtc cgacgctcct tagaaaagct tcaccatcgc
    31981 aggatgtgca atacgtatac cgcgcttgcg gctcatcgaa tcctgaccgc gtcctttttt
    32041 ggtgcgccgc ttaggtcggc acccggcaaa aatgtccacc tcggagaata atctgcccat
    32101 ctccctgcaa gcgacattag gacttctcgg cttcgacctg gtaccccatg tcgcggtatt
    32161 cccgcaattt attccgcagc gttctgatac tgatccccaa ttccttcgcc gcatgcgtgc
    32221 ggttttcttt cacgcgggcc agggtcttga aaataagatc ccgctccatc tcccatagcg
    32281 acccatgcgc ggagggcgtc acgatcgcgt cgccggcctc tacctcccgt accggcaccg
    32341 tgccatcgcc cggcatcaga tgatccacat ccaccgcccc tccggtggct accaggatgg
    32401 cacgctccat gacattttcg agttcacgaa cgttgccttt ccagggacgc tgctggagat
    32461 cggcgatcgc ccgctcggac aacgtcggca tggtgagtcc gtttcgctgt gccgaggagc
    32521 gaaggaaatg tctcgccaga agcggaatat cgcccgaccg atcacgaagc ggaggcaccg
    32581 tgacgggaaa cacgttcaac cgatagaaaa gatcctcacg gaatcggccc tgggtgactt
    32641 cgtgatacag ggagcgattg gtcgtggcga tgacgcgaat attcactgga acaggctcgc
    32701 gcccaccgat acgatcgact tcacgctctt gcaagacgcg caaaagtttg gactgcaagc
    32761 ctaaattcat ttcgctgatt tcatcgagaa acagcgtccc ggtatgcgcc atttcgaact
    32821 tgcctaactt tttctgaatg gctccggtaa atgcgccgcg ttcgtggccg aacaactcac
    32881 tctcgaggag actgtccggg agtgcggcgc aattcaacgc cacgaaaggg cgatgcgcgc
    32941 gcggactgcg cgcgtggata tagcgtgcca ggagttcctt cccggtcccg ctctcgccgc
    33001 tgatcagcac ggtcgcctga ctcgccgcga caccctccag cgtggtgagc aggcggatca
    33061 tgcccgggtc ctgcgtcaaa atcgcccgcg cttccagtgt ggcgggccct tcctgctcgt
    33121 ccggcgccgt ggtggcctgc aggttgagaa tcactcgttc caataggtcc gtcgagaacg
    33181 gctttaagat gtagtcattg gcgccgtatt tcatcgcttc caccgctgtc tccaccgtgc
    33241 cgtaggcggt catcaggaca atgaacgtct ggggggattt ttgtttgatg gccttgacga
    33301 gttccagccc ggtcaggcgc ggcatcttta aatcggtcac caccagccag ggtttcactc
    33361 gttcaatctg ctcaattgcg tcgacgccgt cgatcgcccc ccggacttga tagcctaatc
    33421 tgcggacggt ttcagataag gccgttctca tcgagggttc atcatcgacg atcaggacca
    33481 tccgagcttc ttcggaagtg gcggaggggc cttgtaaatc ctgttgcacg ctcattcgta
    33541 ggtctcctct tcggttgcat tctcgattgg gcgggtttgc acggaccggc cgaccaacgc
    33601 ggcgtttcgc gcagacagcg ttggctgttg tatggaaccc tggggtaatc tcatgacaaa
    33661 tgaggtcccc tgccccggcc tgctctccac atcgatgcgg ccgtgatgag cctcgaccag
    33721 cgcatgtacg atggcgagcc ccaatcccgt cccatggtct ttcgtggtaa aaaacggatc
    33781 gaatacccgc gactgcagct cgagcggaat ccccgtgccg gtgtcactga ccgtgagttg
    33841 gatggcaggc aattctgaga tttgttccgg cgccatacaa gcggacaata tcaggctgcc
    33901 gccttcgggc atggcctgga cggcgttcag aacaagattc agcagcacct gttttatttt
    33961 tccgccgtca caccaaattt tcggaacgcg gggatcgacc tcgcaacgga ccacgatcga
    34021 agccggtgac atggcatggc tcgccagcgt aagtacttcc cgaatcagcg attccgtatc
    34081 ctgccatgcc agcctcgaac agtccggtct ggtatagacg agcaggttcg acaacaaccg
    34141 gtccatggcc tgcaccgcca cggagatatg ctccgcgtat gtccgcaaat gcggctggtc
    34201 acgcagatcc ttgcgcagca tcgacgcgaa caattcaacg ctgcccagag gattccggat
    34261 ctcatgcgcg atgttaccga ccatctgccc catggcctcc agccgattgc gtcgctgcaa
    34321 acggtcttcc agttgtcgaa tgtgggtcac gtcatggatc aacaccagtt tgccgatcaa
    34381 actcccggtt tcatcggtca agtcgctctg cgagatggca atggtcacgc ccgtcggaag
    34441 taccagcggg tactcgccgt ggtctttccg gatttcctcc agcacctctg tcgctcgacg
    34501 ccccctgagg cgggcttgcg gaacccccaa cagacgttcc gtggcgtgat tgcaccgcac
    34561 caccgagtcc tgcgagtccg cgacgatcac acccgtatcc aacgattcca acaccgctgt
    34621 cacatgggcg cgcatttctt ctgtctcacg tagatgttcg cgcaaactgg cattggtttg
    34681 cgcgagttcg agatccatct gctgcagccg tgtcgtcaac gcgtcatacg actgttgaag
    34741 gacggtggca gcctggtcaa agtcatggaa cgcacgtgtc agcagatcgt tgttcgatgc
    34801 ttcggcatcg cgcgctgtca tcgctttttg cctcctgcgg cggaagtggg ctcaggcagt
    34861 tcggtctgca gcacggcggc catacgccga accaggctat cgctgttctc gttcagtgcg
    34921 cgaagcccgt tccttgcgaa ctcttcacgc ttcactcccc gcgccaactg cacgatttga
    34981 aattgggccc aggcttcctg ttcaggctcc aaccccgcaa tcaatgcctg cttatacagg
    35041 gccagcgccg gctcctgacg attcagtctt gccaaaacat cggcatatcg cagccggtcc
    35101 aatgcggaca ggtccatccc cgccttgagt acgctctcat agaggggagc ggcttcggca
    35161 tcccgttttg cctgcctcaa cgcatcggct accttcagct gaaccaatgc gcggtccgga
    35221 tggcgcgggt tgtgtatcaa ccact
//
LOCUS       DAOQRM010000009        34543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_671_length_34543_cov_15.351456, whole genome shotgun sequence.
ACCESSION   DAOQRM010000009 DAOQRM010000000
VERSION     DAOQRM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 34543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34543
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_671_length_34543_cov_15.351456"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1475
                     /locus_tag="PKK81_01780"
     CDS             63..1475
                     /locus_tag="PKK81_01780"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV31625.1"
                     /translation="MAKPKVFVLTDDQAAVAAVRRSIESECEVFVAAWDRTVVVQIHE
                     QTPLVMLLDLGSSSDPAVEERVRVLQEVRQTGHAGKVIACTERTERRLAVRAIQYGAY
                     DVLSKPLDITLLAQLIRRAAGVADLEREARGVMVGGGPEEFSGMLGTSASIHRIFDAI
                     RKVSTNDAPILITGESGTGKELTARAIHERGLRQQGPFIPINCGAIPESLLESELFGY
                     ERGAFTGAVNQKKGKVEFAQGGTLFLDEVGELPNALQVKLLRFLQDHTFERVGGHQPI
                     EMNVRIIAATNVNLKEAIEKGTFREDLYYRLGVVHINVPPLRERGEDVSLMAMAFLRQ
                     AAAHYDKHLEGFTREALEAMRTYAWTGNVRELSNRVGRAVVMAEGTHVTPEDLDIPHQ
                     VARPDGSSISLKVNQQRIETDLIMKAFTLSQGNLSRAAQELGISRSTLYRRLRQYGMD
                     QTLDARRALGVFPRASLSEH"
     gene            1805..2371
                     /locus_tag="PKK81_01785"
     CDS             1805..2371
                     /locus_tag="PKK81_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNV31626.1"
                     /translation="MVSDAPALLSRRGLLRAATAGMALAVVRLALPSVTWASRLPDGR
                     VSLYNLHTDERLSVTYRDESGAYDQDALNALNHFLRCHHTNETTTMDVQLIEFVNLVQ
                     KRVGGRREVHIVSGYRSPEYNEQLIRMGTRAARHSYHVSGQAVDVQIPGVPLRTLREV
                     ALRLGCGGVGYYPRGKFVHLDSGPFRHW"
     gene            2655..3134
                     /locus_tag="PKK81_01790"
     CDS             2655..3134
                     /locus_tag="PKK81_01790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV31627.1"
                     /translation="MMKVSKGDIVSVEYTIRLDDDRVIETTVGEAPLSYTHGQNEILQ
                     GLEAGLDGMERGAAKVITVEPVDAYGEIHPEGFFEVQRERVPAEAQRIGIKLETTAPD
                     GRVVFPYVAEIRPDVIVLDLNHPLAGRTLRFDVRVVDILRGERQLVAGGARGSEKET"
     gene            complement(3131..3493)
                     /locus_tag="PKK81_01795"
     CDS             complement(3131..3493)
                     /locus_tag="PKK81_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31628.1"
                     /translation="MMAWALPVGVPYAAALVEVTELLAHPDHYDHQVVTVSGRVSSFQ
                     LATNRDGHPAFGFLLQDTAGTVKVVGLGKADVREGDYVVVEGIFSRLRQAGRAIVYNE
                     IKATSVQSMARMNPDLVG"
     gene            complement(3695..4864)
                     /locus_tag="PKK81_01800"
     CDS             complement(3695..4864)
                     /locus_tag="PKK81_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31629.1"
                     /translation="MTALLTPELIASTIEQAPIQGRIMLKLLLLQHFDITEEEITHIA
                     ADRPDPRCVAGSKPIHQVVKQDALRDVTSRRDEYRRRVRLRRERLWLQMEAMKRMIAL
                     AEGLIRAGSDLLLTQYKVDAAAVTALKQEARAAVPKPAIRLLNRRWDEDDISAEAYQE
                     ARLGLEIQTQLRLLDKYRKRFELSEREWKSINAAPLQDHEIGHIWGIPAGSLAARKVK
                     HLHQYLQAVQNSLQRSSAKDLPATAPLDLWKETLAVLGERPVERSIAQYDGLERTEDA
                     LVEKLRTFAWGTLGEDLEGKFWLSLVHGASSNAVHSENTRSLFGLQRLMAVLSEIDTS
                     PDALEQEMVTRTTPISKESLALPDEAAQPVVEEVGQMKQHVLTSFMGELHDDLRR"
     gene            complement(4898..5110)
                     /locus_tag="PKK81_01805"
     CDS             complement(4898..5110)
                     /locus_tag="PKK81_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31630.1"
                     /translation="MAENSAVYAIRFPDGSVTLYIDEEYAIDRGVDPAKLVRVEIPRE
                     LFASGSIQEIREYVAVYLENSHQGTA"
     gene            complement(5211..5873)
                     /locus_tag="PKK81_01810"
     CDS             complement(5211..5873)
                     /locus_tag="PKK81_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31631.1"
                     /translation="MSTHALSYAIPDRAAAVYSALLPGLGQLIRGRYGAGLFYGLVTI
                     LLILLSLALGRVSGRAAEVFFFMLLALPWWALQSYDAALGPSESGSDLARSTRTAWAQ
                     GHDVRFLGLLFLVSAGNDALLIARNPDYLLPFFCTRLDGSAGFITKALSPFLHTLVGY
                     GFLRIKKWSLLIYLVYAAYGTTNALVNLTCFGPGRIRNTLLIALIVFTTYVIARRRVF
                     RL"
     gene            complement(5977..6684)
                     /locus_tag="PKK81_01815"
     CDS             complement(5977..6684)
                     /locus_tag="PKK81_01815"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNV31632.1"
                     /translation="MSSPEQAPRRQYVNFTFYKIDPAWRRLPEDERTRGKQEFLRAVE
                     EYQGKVLVIAYTTVGIRGDCDLMLWRISYELELFQEMSTKIMASGLGKYLYNPYSYLA
                     ITKRSIYVDHHTHENQESKRLTVVPGKAKYIFVYPFVKTREWFLLTKAARQGMMDEHI
                     EVGHRFPSVKLNTTYSFGLDDQEWVVAFESDKPEDFLDLVMALRETEGSRYTLRDTPI
                     FTCIRKSLKEALDTLGG"
     gene            6869..7441
                     /locus_tag="PKK81_01820"
     CDS             6869..7441
                     /locus_tag="PKK81_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31633.1"
                     /translation="MGSLFVVLWGSTVSSYAIEVQPTTEQIQAQVERGKLAAGQGVSP
                     DQLHAWFGATEDLAPRGFIMTKLGSLLVMANHLALRALSPTEQDIAQVLANEHVLVNV
                     VLYGDRVNFAADSYIVLEQAGRTVKPSTVRFDARGDRTTAWPQQPAYRAKVIAQFAYA
                     ELDPRAKTTLTVFPSRGGRVSFELDFSRIE"
     gene            7489..8769
                     /locus_tag="PKK81_01825"
     CDS             7489..8769
                     /locus_tag="PKK81_01825"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNV31634.1"
                     /translation="MSKTKTWTAETIAVGSELLLGGRLDTNSLFIADRLAACGIELRY
                     KTTVGDDLSDIVAVLKTALRRARVVILTGGLGPTIDDLTREAVAQATGHRLSRRKAAL
                     DGMIARLVEWGRTPTTTQFRQAMIPARAEILFNPVGTAPGFSLVWKGTFLAALPGVPR
                     EMEPMVRDGVLPQLQAWMRTQKRLSASPMIRRVLHTSGVPESIVDQALVGLVPKGCTI
                     QLGIYASPMEVMVSLTGPAGAEGAVTIEQLLKEAKTRLGDIVYGEEDETMESVVGRLL
                     NEQQLTLAVAESCTGGLIGHRLTQVPGASTYVDRVAVTYSNRAKVEMLGVPSGLLKRH
                     GAVSADVAAAMARGIRERSGASVGVSVTGIAGPGGATESKPVGLVYVGLDGGPGEAMT
                     KEFRFHGGDRTVIKQRSSQAALDLLRRWLIKKGC"
     gene            8769..9386
                     /gene="thpR"
                     /locus_tag="PKK81_01830"
     CDS             8769..9386
                     /gene="thpR"
                     /locus_tag="PKK81_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNV31635.1"
                     /translation="MVRAFLAIELPQELRTTLATIEQDMKRQLERTVDRHVRISWVRP
                     DSMHLTLRFLGDTADESIEPLRVAIEESVSAHQAIPIPLERLGTFPRRQAPRVLWAGP
                     SVSWEQGLEAQRLTSLHRAVEACCRAANFAPEERPFSPHLTLARIKEGERQVGQALAQ
                     NGLLERPVAIGMLPVEAIVLMKSELRPTGSVYTKLWECSLAPSAT"
     gene            9670..11667
                     /gene="uvrB"
                     /locus_tag="PKK81_01835"
     CDS             9670..11667
                     /gene="uvrB"
                     /locus_tag="PKK81_01835"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNV31636.1"
                     /translation="MPPFKLEAPFKPCGDQGQAIEKLTSGILAGKQHQALLGVTGSGK
                     TFTMANVVERVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFISYYDYYQPEAYIP
                     QSDTYIAKDASINDAIDQMRHAATTSLLQRNDVLIVSSVSCIYGLGSPEVYHDMLVYL
                     EEGMETRREKILAKLVDIQYARNDVDFHRGTFRARGDVIEIFPASSEAKSVRIELFGD
                     VVDAIHEIDPLTGKSLGKLPKIAIYPNTHYLIAPDRYERAITGIEEELDARVAAFKKN
                     GQLLEAQRIEQRTKFDLEMIRAMGYCHGIENYSRHLSGRAPGDPPPTLLDYFPKDFLL
                     IIDESHASVPQVGGMYEGDFSRKRTLVDYGFRLPSAVDNRPLKFAEFERMLKQVIYVS
                     ATPGPYELEHAKGEVIEQIIRPTGLMDPLIDVRSAKGQVDNLLAEVRTEAAKGNRVLV
                     TTLTKRMAEDLTEYYHDLGVKVRYLHSDIKTLERAEIIRDLRRGVFDVLVGINLLREG
                     LDLPEVSLVAILDADKEGYLRSHRSLIQTAGRAARNLDGRVIFYGDTITDSMRRAMDE
                     TARRRHIQEAYNEAHGITPESIKKQIPLLDYATGGGNAGQLELAAESVGDYATTGDTE
                     QLIRRLEVEMKAAAKKLEFERAAELRNRIRTLRLKALELAQ"
     gene            complement(11706..11966)
                     /locus_tag="PKK81_01840"
     CDS             complement(11706..11966)
                     /locus_tag="PKK81_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HNV31637.1"
                     /translation="MRKSIGVLLIFILIGGMLGGIFGEILRVMAPNGAIQNIFASNFS
                     PGINPPLTVDLVLLKLTFGFSIKVSLLSVLGMFLGAYLYKQM"
     gene            12389..13738
                     /gene="ffh"
                     /locus_tag="PKK81_01845"
     CDS             12389..13738
                     /gene="ffh"
                     /locus_tag="PKK81_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNV31638.1"
                     /translation="MLDALSEKFERILKKLRGQGVLTEDNIAEALKEVRLALLEADVN
                     FKVVKDFLDRVREKAVGQEVLKSLTPGHQVVKVVWDELRGMMGGERSGISLASRPPTI
                     IMMVGLQGAGKTTTSGKLARLFKSQGKRVLLVAADPRRPAAGDQLASLGHDLGIDVHR
                     FDQIDASRADVVRICQRGVERGQEQGYDLIVLDTGGRLHVDDELMAELVAVKQAVNPH
                     EVLLVADAMTGQDAVNMAGQFDQQVGLTGVILTKVEGDARGGAVLSIRAVTGKPIKFL
                     GMGEKLDALEPFHPDRMASRILGMGDVLSLIEKAQETFSREEAEAAQKKLTSSTFTLE
                     DFRSQLGQMNRLGSFEQILGMLPGGQKLKDLANSGLPEKEMKRVAAMIDSMTTRERRD
                     HTLINGSRKKRIARGSGTSVQEVNRLIKQFLQARKIAKVMSGGAGGRRQLAQMLRGM"
     gene            13845..14162
                     /gene="rpsP"
                     /locus_tag="PKK81_01850"
     CDS             13845..14162
                     /gene="rpsP"
                     /locus_tag="PKK81_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNV31639.1"
                     /translation="MAVHLRLARTGRHKRPMYRVIAADSRKPRDGRFLEILGIFDPLK
                     NPAVPVLKSERVLSWLRHGAQPTVTVRTLLKRHGVWKEFETEKSAKAEKTEKTEKSAK
                     SKK"
     gene            14173..14700
                     /gene="rimM"
                     /locus_tag="PKK81_01855"
     CDS             14173..14700
                     /gene="rimM"
                     /locus_tag="PKK81_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNV31640.1"
                     /translation="MATLDQAELVTIGRIERSFGVRGEARVRSLTDVPGRFESLGQVT
                     IVGPAGKTLDTRVTHVRPGGPTWIMGFEAFTTPEQVAEFRGAFIQVPRGDSPALPADQ
                     YYQCDLIGMVVQDEAGTVLGCLEEVVSMSGNQNFLVRHDGKEMLIPAAKQVVVAVDVV
                     GRVMTVRLPEGFGDL"
     gene            14702..15475
                     /gene="trmD"
                     /locus_tag="PKK81_01860"
     CDS             14702..15475
                     /gene="trmD"
                     /locus_tag="PKK81_01860"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNV31641.1"
                     /translation="MRCAVLTLFPDMVAPVLGQSILKRAQEKGLLEVSVQNLRDHTFD
                     RHKTADDVPYGGGAGMVMKAEPVLQAVDALRAHYQASHPETTLRVIIPSPQGRPFTQE
                     LAQSLAQDERVVVFVCGHYEGMDERVRLALHPEEISVGDYVLTGGELPALVMIDAAAR
                     LVPGVLGDAASAAEESFTEGLLEYPHYTRPAEVRGMAVPEVLVSGHHEAIRLWRRKEA
                     LRNTYLKRPDLLRDRELGSEDRRLLSEVMQESLVQVPVR"
     gene            15492..15905
                     /gene="rplS"
                     /locus_tag="PKK81_01865"
     CDS             15492..15905
                     /gene="rplS"
                     /locus_tag="PKK81_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNV31642.1"
                     /translation="MNRLERIQRSLTKKTTPKFEIGDTVRVHVKVVEGEKERIQVYEG
                     TVIARKGTLNSETFTVRKLSYNVGVERTFPIHSPNVAKVDVVRQGRVRRAKLYYLRTK
                     KGKFAKVEDREFKVESKAQTAAKREAAAAVTAAKA"
     gene            16006..16668
                     /locus_tag="PKK81_01870"
     CDS             16006..16668
                     /locus_tag="PKK81_01870"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNV31643.1"
                     /translation="MEARCRGYRHIAGLDEAGRGPLAGPVVAAAVLLPRRCRLPGLND
                     SKQVGESDRVRLFAEIVRRATGVGIGAATEAEIDRLNILQATRLAMRRALQALPLQPD
                     FLLLDAVTLSGLSVPQRSIIKGDGLSCSIAAASIIAKVTRDRLMVEYHRWYPQYNFAE
                     HKGYGTPEHLRLLREHGPCAIHRRSFASVQQLCVGDARKLCSSVDQSEGPLDRCATDA
                     DW"
     gene            16644..17030
                     /locus_tag="PKK81_01875"
     CDS             16644..17030
                     /locus_tag="PKK81_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNV31644.1"
                     /translation="MRDRRRLVGDEGEGRAEAYLRRQGFRILGRNVRSPLGELDLVAD
                     DQGVLVFVEVKRRRTGTYGGAIEAVDARKRAKLIQLAAQYLAQHRIHDRACRFDVVLI
                     QDDSGTTEPVQHIANAFDVAGDDLRW"
     gene            17133..17801
                     /gene="ftsE"
                     /locus_tag="PKK81_01880"
     CDS             17133..17801
                     /gene="ftsE"
                     /locus_tag="PKK81_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNV31645.1"
                     /translation="MIQLFHVSKYYDRRLALSDVTLEIEKGEFVLLMGPSGAGKSTLL
                     KLLIGAERPEEGQILVQGRSLSKLRSSEIPLLRRKIGVVLQDFRLLPKKTVFDNVSLP
                     LLVQGASTVEIRRKVTDALRSVGLDHKKDLFPPGLSTGEQQRICIARAIVNGPIMLLA
                     DEPTGNLDPELTSEIIELFKSINARGTTIIVATHDPNVLAQVNRRVITLDQGKVVSQE
                     QVCS"
     gene            17798..18697
                     /gene="ftsX"
                     /locus_tag="PKK81_01885"
     CDS             17798..18697
                     /gene="ftsX"
                     /locus_tag="PKK81_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNV31646.1"
                     /translation="MRRVLYLLREAVANVLTNRTTTVVAVATTAFTFACVGVFLLLYV
                     NLRTMASSLEQDIQVMVYLQDDLTEQTRNEIELQLKADRAVGSLTFVSKERALADFQS
                     QFPAESHLLQGLGQNPLPASFVVTLAVESRSSDAMRRWANRTQSIPGVAQVQYNQEWV
                     EALAGIVRYIEVAAIIVGLILSAASVTIIANTIRLALYSRREEIEILGLIGASTTFIR
                     VPYLLEGAALGLLGSALSLVILKAGFELFRHEIRSASRFLGVDALLTFFPFQMCVVLV
                     CVGLFLGCAGSFLSLLRFGEGRA"
     gene            18694..19884
                     /locus_tag="PKK81_01890"
     CDS             18694..19884
                     /locus_tag="PKK81_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNV31647.1"
                     /translation="MKWARWGCVVVAAHLVLWTVPAWAERKDSYADKIEQEKKTLEKL
                     RGTIVEKRKKADEAEKKRESVLQGLQSLDERLVRYRQEHQDVVKKLKKKDVEIEQMSV
                     QLSRLSERIDERQDAIAARLRVQYVEGRFWHLRTLLAASSSGDFQRRFRYLSAVTQRE
                     YEIMETYRKDAERIAEVERSREEARQGILAYKASTEEKLAQIQGLKKQKRVYLAKITQ
                     EKESHDRAVEELERSANRVDSLLKELETRRRAALATRPPSVTGGLRALRGTLLWPTEG
                     QVVSYFGRQKHPTFNTYIQRKGIEIRAAEGSNIRSVLAGQVVYADWLKGYGLVIIMDH
                     ANGVFSLYAHASKILTSVGARIEAGEAIGETGDTGMTGENTLYFELREGAEPVDPLVW
                     LSKR"
     gene            19911..21260
                     /locus_tag="PKK81_01895"
     CDS             19911..21260
                     /locus_tag="PKK81_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNV31648.1"
                     /translation="MEQRRSRRWLYLLLMVTVALGIGIVLEKGLERTGHASETYEELR
                     TFSEVLTQVQKHYVDDTKVKDLVQGAIRGMLSTLDPHSAYMTPEMYKEMQVETKGEFG
                     GVGIQIGVKENRLAVISPIEGTPAHRAGIKAGDFITKVNDEPTKDLTLMDAVQKMRGP
                     KGTKVNLTIQRDGTADPLAFSLVRDTIKIESVKFKVLDNTIGYVRLTQFQEATGRDLS
                     RALKQFKEQKVQGTILDLRNNPGGLLTAAVDVSEQFVGNGKLIVYTKGREGKKDEWFS
                     KTKETLEDSPMIILVNEGSASASEIVAGALQDWGRAVIVGTTSFGKGSVQTILPLGDG
                     SGLRLTTAKYYTPKGRSIQSTGITPDIVVKLQTQTVAKAGEKDGKESEPKTAKAPAAG
                     KDPSPSAKPGEEPAHKNGAVSAGDGAGDISLDDDVQLQKAVELLKTWKIFKELRPAA"
     gene            complement(21235..21726)
                     /locus_tag="PKK81_01900"
     CDS             complement(21235..21726)
                     /locus_tag="PKK81_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNV31649.1"
                     /translation="MDDRTHQLAQIFRAMANLLASQRANPYRVRAYRKAADSILTVTG
                     DLADLASRHQLQEIPGIGRDLAVKIEEFLATGTIRAYEELKTPLPEEVAGWASLPGLS
                     DALVSYLYFRLNIRTLADLESLVASHLLRTQPGFSGSEDTLLAAIRERIGTVPTTPPA
                     AAP"
     gene            21855..22061
                     /gene="thiS"
                     /locus_tag="PKK81_01905"
     CDS             21855..22061
                     /gene="thiS"
                     /locus_tag="PKK81_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNV31650.1"
                     /translation="MIIHVNGESRGIGDGETVAALLHELEIRADRVAVELNLEILDRK
                     DFETRGLREGDRVEILSFIGGGAR"
     gene            22070..22849
                     /locus_tag="PKK81_01910"
     CDS             22070..22849
                     /locus_tag="PKK81_01910"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNV31651.1"
                     /translation="MDTINDRLVIAGREFTSRLWVGTGKYKDFVETKKAIEASGADVV
                     TVAVRRVNITDRSKENLLDYLDPKKYIILPNTAGCYTVEDAVRYARLARAAGVSDLVK
                     LEVLGDEKTLFPDTAGLIEAAKILIKEGFIVLPYTNDDPIVAKKLVDIGCPAVMPLAA
                     PIGSGLGIRNPYNLKIIMETVKVPIIVDAGVGTASDAALAMEYGADAVLMNTAIAGAQ
                     DPIAMAEAMKYGVWAGRLAYKAGRIPRKLYATASSPIEGML"
     gene            22979..23605
                     /gene="thiE"
                     /locus_tag="PKK81_01915"
     CDS             22979..23605
                     /gene="thiE"
                     /locus_tag="PKK81_01915"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNV31652.1"
                     /translation="MPSVDFRLYLVTDRQQTAGRPLVSVVGRAVSAGVRAVQLRERDL
                     PIRELLSLALDLQRELSDMQVFINDRVDLAVALGCRGAHLRESSLPAPVVRTLLQPSQ
                     LLGLSVHSIQDAVAAERHGADFVVLGPIYDTPSKREYGAPLGLQVLEQAARAVNIPIF
                     AIGGMTASRTREVRQAGAFGVAVLSSILSASNVEVATEKFLSAIERES"
     gene            23761..25530
                     /gene="arc"
                     /locus_tag="PKK81_01920"
     CDS             23761..25530
                     /gene="arc"
                     /locus_tag="PKK81_01920"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HNV31653.1"
                     /translation="MAESKGSSGRLRSFRDSVKRLTKSLSDGDGVVTHKNDEQAREVE
                     KLRIQIQSMEEEIRRLYQSRYQLEQTTKQNEKLVATLQEAKSQIETLRAEVEKLTAPP
                     STYGIFSSLDTDGTGNVYVSGRKMKVSLHPSIKAKSLRKGQEVILNEALNVIEVRGFD
                     VQGEVVRLKDVLEGNRALVTLHFDEEKVAELGEPLLSERLSVGDHLLYDPRSGCVIEK
                     LPKSEAEELVLEEVPDVDYEHIGGLQKEIEQVRDAVELPFLYPHIFANYKLSAPKGVL
                     LYGPPGCGKTLIAKAVASSIAKKLGHLKDKQLRSYFLHVKGPELLNKYVGESERQVRE
                     VFKKAKERADDGHPVIVFFDEMDALFRTRGTGISSDIESTIVPQFLSEIDGVERLTNV
                     IVIGASNRQDLIDPAVLRAGRLDVKVKVGRPDATAARDIFSKYVSTDLPFAEDDLKRH
                     GGDARALVDSLMNMTVDAMYATTDENKFIEVTYANGEKEVLYFKDFASGALIEGIVSR
                     AKKFAVKRAIAQEGTGLRAEDLIRAIREEFKEHEDLPNTTNPDDWAKVAGKKGEKIVH
                     LRTISGGPAEARQIETVNTGHYL"
     gene            25542..27041
                     /gene="dop"
                     /locus_tag="PKK81_01925"
     CDS             25542..27041
                     /gene="dop"
                     /locus_tag="PKK81_01925"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HNV31654.1"
                     /translation="MSDNPTHNPSRVIGTETEFGIASRDPNAADPVANSIHLIGYYPN
                     LPAPHAVWDYENENPLLDARGFEVDGERERPGPDYNRQLNKVLANGGRLYVDGAHPEY
                     STPECLNAREVVAFERVGERIVAQALEGITKARGRDQFVLYKNNSDGKGNSYGYHENY
                     LVSRAVPFERITQVLTPFFVTRSIYAGSGKVGAENQTSPVDYQISQRADFFETLVDLN
                     TMVRRPIINTRDEPHSDSAKYRRLHVIVGDANMAEVSTYLKVGTLSIVLELLEAGADL
                     PRISLADPVNAIKQVSRDVQVKESVKLSDGSSSTAIAVQRAYLKAAQSYYACHELNQV
                     TKDVLVRWEDVLDRLERDPRSLVRELDWVAKRYLIESYMERKSCGWDDPRVRLMDFQY
                     HDVRPDKGLYYTLERNHRIERVVLDHEIARAEFNPPVGTRAYFRGQCIKKYPTVVYGA
                     SWTSVLFDTGQNKIKRIPLMDPLRGTESLTGELLAQAETASALLAKLST"
     gene            27055..27879
                     /gene="prcB"
                     /locus_tag="PKK81_01930"
     CDS             27055..27879
                     /gene="prcB"
                     /locus_tag="PKK81_01930"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HNV31655.1"
                     /translation="MKHSFFFSHHEGSSFFDFLTQHHPELRPGLSEIAIQRAALPVDF
                     GRPGTIPVPHGTTVLALKYRDGAIIAGDRRATEGFQIADRRIEKVFRIDDYSAMAIAG
                     AAGPCIEMAKLFQTELEHYEKLEGVQLSCEGKANKLGQMVKANLPMVFQGLVVMPLYV
                     GYDLKRNEGRIFKYDITGGRYEESDHHSIGSGGKDARNTMREHYRSGLQEEEALRVGL
                     LALYNAADEDVGTGGPDLVRGIYPTAKIVSGAGITDVPEDRVRALFETMIAERRRS"
     gene            27883..28566
                     /gene="prcA"
                     /locus_tag="PKK81_01935"
     CDS             27883..28566
                     /gene="prcA"
                     /locus_tag="PKK81_01935"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HNV31656.1"
                     /translation="MPLPYYVSPEQMMQDKAEYAKKGIAKGRSIIALEYIDGILLAAD
                     NPSSSLHKVSEVYDRIAFAGAGKYSEFEHLRKAGIRHADLTGYMYSREDVSARTLSNA
                     YSQSLGTAFSTDVKPLEVEILVVQVGVNGQANEIFRISFDGSIVDEKNLAVIGGRSEA
                     VQQYLREHATPQPPTLKDGLRRCLASLEQVATQKIPSENLEVAVLDRNRLGRKFKRLS
                     SADISQLFA"
     gene            28698..30074
                     /gene="pafA"
                     /locus_tag="PKK81_01940"
     CDS             28698..30074
                     /gene="pafA"
                     /locus_tag="PKK81_01940"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HNV31657.1"
                     /translation="MQQRIFGLENEYGLIFSPNGKIYLPMEKVLGYIFEGLIPNSWPS
                     NAFLVNGARFYQDTGCHPEYSTPECDNILDLVIHDKAGERLLEACLPAAEERLREEGL
                     SGEIYIFKNNTDSLGNTYGCHENYLMRRDVDFWKVTEQLIPFFVTRQIYSGAGKVLKV
                     SGKPQYFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAEKYRRLHIIVGDSNMSEY
                     ATYLKVGTATLVLSMIEAGYSVSGMELEDPVKAIREISRDPTLNKKVKLDDGRQMTAI
                     EIQRVYVKRAKEFLSEQEHDPVLDDVWEKWVSVLDRLEEDPMQLVHEVDWVMKKHLIQ
                     SYTDKKDCGWDDPRVFLLDLQFHDVKRTRGLYYLMENRGMAARVVEEEAVQRAMSVPP
                     QTTRAKVRGDFIRFARAKNRSYTVDWTYLKLNGYWEETILCMDPFSAVNRRVEELLSQ
                     VSGARLYR"
     gene            30107..31093
                     /locus_tag="PKK81_01945"
     CDS             30107..31093
                     /locus_tag="PKK81_01945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV31658.1"
                     /translation="MSFLDRFLIISVVLLTSVFPVELFPNTGPAPRGGDGQFSGKQGQ
                     VILINVPDIKDAASVKGQFLGRKVTLFPDPSAGGGAGYVGLLGIDLQDEPGAHELTID
                     AQLGEQTRHFSYQVLVLKEKFAVEHLKLPKDKVDLDEKAAARWKAEQEQVRKALAEES
                     AMRLWQAGFIEPVHGKRTGIFGSVRIMNGQARNPHNGEDIGAPMGTDVMASNDGVVRL
                     IVDHIFSGRGIFVDHGLGLYSMYFHLSDVLVKEGDLIKAGQIIGKVGATGRATGPHLH
                     WGMKVNGARVNPYALLELPFPQNPAVARPVLASPTRPAQGESSATAVGGDTR"
     gene            complement(31164..33593)
                     /locus_tag="PKK81_01950"
     CDS             complement(31164..33593)
                     /locus_tag="PKK81_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31659.1"
                     /translation="MHVLLIEDNEDDAELIQQALCERSTDAVTLNWADRLQTGIKKLA
                     ECPVDAVLVDLSLPDSQGLETLDRVRAQAHDAPVIVLTGLDNDVVAEKSLLHGAQDYL
                     VKGRLTGDALRRAIRYAMGRHRVEQALRKSEERFHLTCLATRDGIWDWEITSGVTWFN
                     EAHQSVYGSAPEKLRRGHIPWAEQIHSEDQTAVLTNLAQVLQSDQKLWTAEYRFRRAD
                     GTFAYVIDHGYVLRDHEGRPYRMIGAKADITERRQAETMHAVQLAVGLALEESASLGE
                     AVPKILRTMCELQDWTLGALWLVHSQDKTLRCNALWHQPSLPAENFARIYRGLALRQG
                     AGLAGQVWKTGDATLCPDILNEADCPILHAAQKADLHGGIAFPIHTGKEILGIMEFLT
                     YERLRPSSSRLERASELGAMITQFLHRKDLERQLRQAQKMEALGRMAGGIAHDFNNLL
                     TIINSWAELLTDEPGQTTRTQRGMAQIREAGNKATALTRQLLAFTRHQIVERQPLNLN
                     DRVTDIVELMKRVIGEDIQLTLTLDPALGRIKADPGQIEQVVMNLVVNARDAMPQGGR
                     LDLETKSVSITHSDPLWPDPLTPGPYVTLAVRDTGCGMDADTVAHVFEPFFTTKELGK
                     GTGLGLSTVYGIVRQSGGTVGIESEPGRGTTFTIYLPCIDEDSESPRPVAQPRSIIEQ
                     SKTILLVEDNDMVRGLARTVLVGQHHSVLAARTGEEALQLVRQRGGKISLVITDMVMP
                     GMGGIQLAAELRTLQPEINIILTSGYSDREGSMLEQLDARTTFLPKPYTPDSLIKAVN
                     AALDPTLSNQT"
     gene            complement(33597..34037)
                     /locus_tag="PKK81_01955"
     CDS             complement(33597..34037)
                     /locus_tag="PKK81_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31660.1"
                     /translation="MTMRPFDILIAEDNEDDILLIREAFETVNMANRVAVVRDGEEAL
                     AYLRGEGPFSQCPFPGLVLLDINMPKKTGLEVLIEIKSDPRFRPLPVVMLTVSEREED
                     IVRSFEQGACSYIRKPVTFSRFLSVVKEFELYWTLVSRVPTLKR"
     gene            complement(34034..>34543)
                     /locus_tag="PKK81_01960"
     CDS             complement(34034..>34543)
                     /locus_tag="PKK81_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV31661.1"
                     /translation="QRMDPPVDDIDAEQLVHEVLRRLEKRIKETDARITIRSPLPRLR
                     VNTTWAIQGIYNLVANALKFASAGQAPDIEIAPHSGMDLEGNELIGLVVRDRGPGVAP
                     EQRDRIFELFRRAVGREIEGTGAGLAIVRQVAERHSGRAWVEPREGGGSEFFITFGVN
                     QSSPRKVQR"
BASE COUNT         7137 a         9270 c        10956 g         7180 t
ORIGIN      
        1 agtgcgacga tggtcagccg tcccatcgtg ccagtgtctc accagctgaa aggagccgaa
       61 tcgtggcgaa acccaaggta ttcgttctca cagatgatca ggcagccgtt gccgccgtgc
      121 gccgctcgat cgagagcgag tgtgaggtgt tcgtggccgc atgggatcgg acggtggtgg
      181 ttcagatcca cgaacagacc ccgctggtca tgcttctgga tttggggtcc tcgtccgatc
      241 ccgctgtcga ggagcgtgtg cgggttctgc aggaagtgcg acaaaccggg catgccggaa
      301 aggtcatcgc ctgcacggaa aggacggagc gccgcctcgc cgtccgcgcg atccagtatg
      361 gagcctacga cgtgttgtct aaaccgctgg atatcaccct tctggcacaa ctcatccggc
      421 gggctgcggg agtggctgat ctggagcggg aggcccgagg cgtcatggtt ggaggaggcc
      481 ctgaagaatt cagcggtatg ctcggcacca gtgccagcat tcaccggatc ttcgacgcca
      541 tacgaaaggt ttccacgaac gatgcaccga tcttgatcac cggggaaagc ggcacaggca
      601 aggagctgac ggcgcgcgcg atccacgaga gagggttacg gcagcaggga ccgttcattc
      661 ccatcaattg cggggcgatt ccggaaagtc tgctggaatc ggagctcttc gggtatgagc
      721 gtggcgcctt tacgggagcg gtgaaccaga agaaaggcaa ggttgaattt gcccaagggg
      781 ggacgctctt cctcgatgaa gtgggcgaac tcccgaatgc cttgcaggtc aaattgttgc
      841 ggtttctgca ggatcacacg tttgagcggg tgggcgggca tcagccgatt gaaatgaacg
      901 tgcgaatcat cgccgcgacg aatgtgaatc tgaaggaggc cattgaaaaa ggaacgttcc
      961 gtgaggacct ctactatcgg ctcggggttg tccatatcaa tgtgcctccg cttcgggagc
     1021 gcggtgagga tgtgtcgttg atggccatgg cgttcctgcg tcaggcggca gcccattacg
     1081 ataaacatct ggaaggtttt acccgcgaag cgctggaggc gatgcgcacc tatgcctgga
     1141 cggggaatgt gcgtgaattg tccaacaggg tcggtcgtgc ggtggtgatg gcagaaggca
     1201 cgcacgtgac tccggaggat ctggatatcc cgcatcaggt ggcgcgaccg gacggcagct
     1261 cgatttcgct taaggtgaat caacagcgga tcgagaccga tctgatcatg aaggccttca
     1321 cgctgtcgca gggaaacttg agtcgagccg ctcaggaatt gggcatcagt cgttcgacgc
     1381 tgtatcgacg gctgcgccag tacgggatgg accagacgct tgatgcgcgg cgagcgctcg
     1441 gggtgttccc gcgcgcctcg ctgtcagagc attgatgatg cggccggcac tgtgagattg
     1501 atcgtggctg gtgtgccgta gtgatacaat cctgtcactc tgagatcgat gcgagcgcat
     1561 aggtgtgtaa aacggttgac cgtcgtacag gtatttggta catacaacag tctccgatta
     1621 cctgctgatc atgctgttct ccggggtacg gttcctcacc tcggcttgtg gcccatcctg
     1681 tgacaatgta cgcgtgaatt ggttgtttca cgcatgacag gttgagaaat ctacgggtcc
     1741 agccttgtca atcacgtgct gacggcgcga aatggtaagt atgttttcga gtgggagtca
     1801 atcgatggtc tccgatgcac cagccctctt gagccggcgc ggattgttgc gcgccgctac
     1861 ggccgggatg gcgctggctg tggttcgcct ggccctgccg tcggtgacgt gggcgtcacg
     1921 gttacccgat ggccgggtga gtttgtacaa tcttcacacc gatgaacggt tgtcggtgac
     1981 gtaccgtgac gagtctggcg cgtacgatca ggacgcactc aacgcgctca atcattttct
     2041 gcgctgccac catacgaacg aaaccaccac gatggacgtg cagctcatcg aattcgtcaa
     2101 tcttgtccaa aagcgggtcg gcggccgtcg cgaagtgcat atcgtatccg gttaccggtc
     2161 tccagaatat aacgagcagc tcatcagaat gggcacgcgt gcggcgcgtc acagctacca
     2221 tgtgtcgggg caggctgtgg atgtgcagat tcccggtgtg ccgctgcgga ccttgcgtga
     2281 ggtggcgtta cggctcggtt gtggcggggt gggatattac ccgcgtggca agtttgtcca
     2341 tctcgattca ggtcccttcc gtcactggta acgaccagtt cttgccgttc ccttttcgtt
     2401 cacggcgctt ctcagagcat caccggatcg cctgctctcg gtgtataggt ccgatcacct
     2461 gccggcatga ggggcattcc tgctattccc atgaccccat gatcccgtcg tgatagggtg
     2521 cttctggatg ccgtctctag cgcattgccg tctgttatcc cgaacacgag cggaggctgc
     2581 aggacatggc ggactgagat gggccgaagc ggaagtgatg gcaacccatg gggactgccg
     2641 gtgaggggga ctgaatgatg aaggtatcta agggcgatat cgtttcagtc gagtacacca
     2701 ttcgcctgga cgatgaccgt gtgattgaga caaccgttgg tgaggcgccg ctgagctaca
     2761 cccatgggca gaatgagatc ttgcagggac tggaagccgg gcttgatgga atggagcgtg
     2821 gagcggccaa ggtcatcacc gttgagccgg tcgatgccta tggagagatt caccccgagg
     2881 gattctttga ggtgcagcga gaacgtgtcc cggcagaggc gcagcggatc ggaataaagt
     2941 tggaaacaac ggctccggat ggccgagtgg tattccctta cgtcgccgag atcaggccgg
     3001 acgttattgt gcttgacctc aaccacccgc ttgccggccg gacgttgcgg ttcgatgtga
     3061 gagtggtgga tattctgcgt ggtgagcgcc agcttgtcgc ggggggagcg cgaggaagcg
     3121 agaaagagac ttaaccgacc aggtccgggt tcatgcgggc catagactgg accgatgtgg
     3181 ctttgatttc gttatacaca atggcgcggc cggcctgacg aagacgactg aatatccctt
     3241 ccaccaccac gtaatcacct tcccgcacat ctgcttttcc gaggcctaca actttcaccg
     3301 ttcctgcggt gtcctgcagg agaaatccga atgccgggtg accgtcacga ttggtggcga
     3361 gctgaaaact gcttacgcgc ccgctgacgg tgacgacttg atgatcatag tgatcggggt
     3421 gggccaggag ttctgtgact tcgactaagg ctgcggcata tgggacaccg accggcaatg
     3481 cccaagccat catcgtcaac gtaccgagca gacacagggt gccaatggcg gtaggccgga
     3541 aagaccgtct gtctctacgt tgcagcatga tagttggatt atacaaaatt cactccaggc
     3601 tgagaaggag gacgtgaaaa aacttttctg aacaactggg acgatgtgca accagcgtcg
     3661 taagcaggcg ccggcgcgct gccagagcac gcgactatcg ccgcagatcg tcgtgcaact
     3721 cacccataaa gcttgtcagc acatgttgtt tcatctgccc gacttcttcc acgaccggtt
     3781 gggcggcttc atcgggcagt gccaaggatt ccttgctgat cggggtcgta cgggtcacca
     3841 tttcctgctc gagcgcatcc gggctggtat cgatttcgga cagtactgcc atcaggcgtt
     3901 gcaggccgaa gagcgagcgg gtattttcag aatggacggc attcgaactg gcgccatgaa
     3961 cgagtgacag ccagaatttc ccttcgagat cctcccccag ggtgccccac gcgaaggttc
     4021 gaagcttctc cacgagcgca tcttcggtcc gctctaaccc gtcatactgc gcaatggatc
     4081 gctccaccgg tcgttccccc agcacggcga gggtttcttt ccagaggtca agcggcgcag
     4141 tcgccggaag gtcctttgcc gacgaccgct ggagagaatt ttgaacggcc tgaaggtact
     4201 ggtggagatg tttgaccttc ctcgcggcga gacttccggc cggaatgccc cagatatgcc
     4261 cgatttcgtg atcctgcaag ggcgcggcat taatcgattt ccattcccgt tccgagagtt
     4321 cgaaccgttt ccggtacttg tccagcaagc gcagctgtgt ctggatctca aggccgagcc
     4381 gggcttcctg gtacgcttcg gcagaaatgt cgtcttcatc ccatcgacgg ttcaagagcc
     4441 ggatcgccgg cttgggaact gcagcacggg cctcttgttt gagggcagtc acagctgcag
     4501 cgtccacctt atactgtgtg agcaacaggt ccgacccggc gcgaatcaac ccctcggcga
     4561 gcgcgatcat gcgcttcatg gcttccatct ggagccagag ccgctctcgt cgcaaccgga
     4621 cccggcggcg atattcgtcg cggcggctcg tcacatcgcg cagcgcgtcc tgtttcacaa
     4681 cttggtggat cggtttcgaa cccgcgacgc aacgtggatc gggccgatca gccgcgatgt
     4741 gggtgatttc ctcctccgtg atatcgaaat gctgcaacag cagcagcttg agcatgatgc
     4801 ggccctgaat cggggcctgc tcgatggtcg aggcgatcag ttctggggtg agcaaagcgg
     4861 tcatagtgtg gcccctcgtg cgacgtaaaa ggttcgctca ggccgtgccc tgatggctgt
     4921 tttcaaggta gaccgcgaca tattcacgaa tttcctggat gctgccgctc gcaaacaatt
     4981 cccgaggaat ctcaacccgc accagcttgg cagggtcgac gccccggtcg atggcatatt
     5041 cttcgtcgat atacagagtc acggaaccgt caggaaagcg gatggcatag accgcgctgt
     5101 tttcggccat ggaggctgct ccgtctgatg ttgtggaacg gtatggctat tacaggtaac
     5161 acagccccag cccgagtgtc aaaacgcgtc gggggttcga acaggggtgt tcagagccgg
     5221 aagacccgtc tccgtgcgat cacataagtc gtaaagacaa tgagggcgat gaggagtgtg
     5281 ttccggatcc ggccggggcc aaagcaggtc agattgacga gggcattggt ggtgccgtaa
     5341 gcggcataga ccagatagat caggagggac catttcttga ttcgtaagaa gccatacccg
     5401 accagggtat ggagaaacgg tgacagcgct ttggtgatga acccggccga gccgtccagc
     5461 ctggtacaaa aaaacggcag caggtaatcg ggatttcttg cgatcaagag ggcatcgttc
     5521 ccagcactga ccagaaagag cagtccgaga aaccggacat cgtgtccctg cgcccaggcg
     5581 gtgcgggtgc tgcgggcaag gtccgaaccg ctctcggacg gcccgagtgc ggcgtcgtag
     5641 ctctgcaggg cccaccaggg aagggccaac aacataaaga aaaagacttc ggcggctcga
     5701 ccggagaccc gcccgagcgc caggctcagg aggatcagga gaatggtgac caggccgtag
     5761 aagagtcctg ccccatatcg tccccgaatg agttgtccga gcccggggag tagggctgag
     5821 tagacagctg cggcccgatc cgggatagcg taagaaaggg cgtgggtgga cattgatcga
     5881 gaacgcgtga gatcagacga cgtgtcggga tagaaacggc acggccttcg atcaagggat
     5941 cgaaggccgt gtccacagac tgctgcgcaa ccgttattag ccgcccaggg tgtccaaggc
     6001 ttctttcagg ctcttgcgga tgcaggtgaa aatcggggtg tcgcgcaggg tgtaccggct
     6061 accttcggtt tcccggagcg ccatgacgag gtccaggaag tcttccggct tgtcgctctc
     6121 gaaggccaca acccattcct gatcgtcgag gccgaaggag taggtggtgt tgagcttgac
     6181 cgaggggaag cgatggccga cttcaatgtg ttcgtccatc atgccctgcc gcgccgcctt
     6241 ggtgaggagg aaccattcgc gggttttcac gaacggatag acgaaaatgt acttggcttt
     6301 gccggggacc acggtgagcc gcttgctttc ctggttctca tgcgtgtggt ggtcgacata
     6361 aattgaacgt ttggtgatcg cgaggtacga atagggattg tacagatact tgcccaatcc
     6421 ggatgccatg atcttcgtgc tcatttcctg gaacagttca agctcgtagc tgatgcgcca
     6481 gagcatcaga tcacagtcgc ctcgaatacc caccgtggtg taagcgatga cgagcacttt
     6541 tccctgatac tcttcgacgg cacggagaaa ctcctgcttt ccgcgtgtgc gttcatcttc
     6601 aggaagccga cgccatgcgg gatcgatctt atagaaggta aaattgacgt attgacggcg
     6661 gggtgcttgt tcggggctgg acatgaggaa ccctccaggc aacttcttga taatgtaaaa
     6721 gaataagagt tagctcttta gcatttcgtc tctggggctg tcaacagagt ccggggtgcg
     6781 cggggctcga attgacaggc ccacatcgat ctgatactgc ctgcatacga tgcgcggcgt
     6841 tcgaagattg ccggccggat tctggctcgt gggaagcctc ttcgtggtcc tgtgggggag
     6901 caccgtctcc tcatatgcca tcgaggtgca gccgaccacc gaacaaattc aggcgcaggt
     6961 ggagcgtgga aagctggctg ccggccaagg tgtctctccg gatcagctgc acgcctggtt
     7021 cggtgcgacc gaagaccttg ccccgcgcgg tttcatcatg acgaagctgg gaagcctgct
     7081 ggtgatggcc aatcaccttg ccctgcgcgc gttgtcgccg accgaacagg atattgccca
     7141 ggtactggca aacgagcatg tgctggtcaa tgtggtgttg tacggagacc gtgtcaattt
     7201 tgccgccgac agttacattg tgctggagca ggccggtcgg acggtgaagc cctccaccgt
     7261 tcgatttgac gccaggggcg atcgcacgac ggcgtggccg cagcagcctg cctaccgtgc
     7321 gaaggtgatc gcgcaattcg cctacgcgga actcgaccca cgtgcgaaga cgacgctcac
     7381 ggtatttcct tcacgcggag gccgggtcag cttcgaactg gacttctcgc ggatcgaata
     7441 gtatcagtcc ctctccatcg ctctgcccat cgcaaaggtg tatcccgcat gagtaagacc
     7501 aaaacctgga cggctgaaac catcgccgtc ggatcggagt tgctgctcgg cggccgcctc
     7561 gacacgaatt cactcttcat tgccgatcgt ctcgccgcct gcggaattga gcttcgatat
     7621 aagaccacgg tcggcgacga cctgtccgac atcgtggctg tgttgaagac ggcgttacgg
     7681 cgcgcccgcg tggtgattct caccggtggg ttggggccga cgatcgacga cctcacgcgg
     7741 gaagccgtgg cgcaagcgac gggccaccgc ttgagccggc gcaaggctgc cctggatggg
     7801 atgattgcac ggctggtcga atgggggcga actccgacca ccacgcagtt tcgccaggcc
     7861 atgattcccg cccgtgcgga gattctcttc aatccggtcg gaacggcgcc ggggttttcg
     7921 cttgtctgga agggcacgtt tctcgccgcc ttgcccgggg tgccgcgtga gatggaaccg
     7981 atggtgcgtg atggcgtgtt gccgcagttg caggcgtgga tgcgcacaca gaagaggctc
     8041 agcgcgtcgc ccatgatccg gcgggtgctc cacacgtccg gtgtgccgga atcaatcgtg
     8101 gaccaggccc tggtcgggct tgttcccaaa ggttgcacga ttcaactcgg catctatgcg
     8161 tccccaatgg aagtcatggt gtcgctgacg ggaccggctg gagcggaggg ggctgtcacc
     8221 atcgagcagt tgttgaagga agccaagacg cggctcggtg acatcgttta tggagaagaa
     8281 gatgagacca tggaatcggt ggtcggtcga ttgctcaacg agcagcagtt gacgcttgcc
     8341 gtggccgaat cctgtaccgg tggactgatc ggccatcggc tgacgcaggt gccgggcgca
     8401 tcgacatatg ttgatcgagt ggctgtcacc tatagcaatc gtgccaaggt tgagatgttg
     8461 ggtgtgccgt caggactgct caaacggcat ggggcggtca gcgcagacgt ggccgccgcg
     8521 atggcgcggg gcattcgtga gcggagcggc gcctccgtcg gcgtgagcgt gaccggcatc
     8581 gccgggcccg gtggcgcgac cgagagcaag ccggtgggcc tggtctatgt ggggcttgac
     8641 ggcgggccgg gcgaggcgat gacgaaagag tttcgttttc acggcggtga ccgtaccgtg
     8701 atcaagcaac ggtcctcgca ggcggcgctt gatttgcttc gtcgctggtt gatcaagaag
     8761 gggtgctaat ggtccgggca ttcctggcca tcgagttgcc acaggaattg cgcacgacgc
     8821 ttgccaccat tgagcaggac atgaagcgac aactcgagcg aacggtcgat cgccatgtga
     8881 gaatcagctg ggttcgaccg gattcgatgc acctcaccct cagatttctc ggcgacacag
     8941 ccgacgaatc gattgagccg cttcgggtag ccatcgaaga gtccgtgtca gcgcatcagg
     9001 cgattcctat tcctctcgaa cgactgggta cgttcccgcg ccggcaggca ccacgggtgc
     9061 tgtgggcggg accctcagtg agttgggagc agggccttga ggcgcaacgg ctcacaagcc
     9121 tccatcgtgc ggtggaggct tgttgccggg cagcgaactt cgcgccggaa gagcggccat
     9181 tcagtccgca tttgacgctc gcgcggatca aggaagggga gcggcaggtc ggacaggcgc
     9241 tggctcagaa cgggttgttg gagcggccgg tggcgatcgg catgttgccg gtcgaggcaa
     9301 tcgtcctcat gaaaagtgaa ctgcgtccca ccggctcggt ctatacgaaa ctctgggaat
     9361 gttcactagc cccttctgcg acgtaagaac cgtaacgccc tccaaacttt gccggaagtg
     9421 gcatcatgct ggggtcgccc cgccactatt gctaggcccg ccgtcagcag gaacggtcga
     9481 gtgcgtcgtt tctgattcat ggacagtctg ggtatggaag ttttccgacg cccaaggccc
     9541 ggaatggtat gatgatcggt gataaactac gagcccatac gcaacgcaat ctactaccgg
     9601 tccggcggga gtccagcaag ccgcctcaag cactacagca tgcagatcta gaaagctggt
     9661 ctattcaata tgcctccgtt caagctcgaa gctccattca aaccctgtgg tgatcagggg
     9721 caagccattg agaagctgac atccggaatc ctggccggga agcagcatca ggcgttgctc
     9781 ggggtcaccg gctccggcaa gaccttcacg atggccaatg tcgtcgaacg cgtccagaag
     9841 ccgaccctcg tcctcgtgca taacaaaact ctggccggcc agctgtatca ggagttcaag
     9901 cagttcttcc cacacaatgc cgtcgagtac ttcatcagtt attacgatta ctaccaaccc
     9961 gaagcctaca tcccgcagag cgacacctac attgccaagg atgcctcgat caacgatgcg
    10021 atcgatcaga tgcgtcacgc ggcgacgaca tccttgctgc agcgcaacga tgtgctgatc
    10081 gtgtcctcgg tctcctgcat ctacggcctc ggatcgccgg aggtgtacca cgacatgttg
    10141 gtctacctgg aagaggggat ggagacgcgg cgtgaaaaga ttctagcgaa gctggtcgac
    10201 attcagtatg cgcgcaacga cgtggatttt caccgcggca cctttcgcgc acgcggcgat
    10261 gtcatcgaga tttttccagc ctcgtcggaa gcgaaatcgg tgcgtattga attgttcggc
    10321 gatgtggtgg acgccattca tgagatcgat ccgttgaccg gtaaatcgct gggcaaatta
    10381 ccgaagattg ccatttaccc gaatactcac tatctcattg cgccggaccg gtatgaacgg
    10441 gcgatcacgg gcatcgaaga agaattggac gcccgcgtgg cggcattcaa gaagaacggt
    10501 cagttgttgg aagcgcaacg gatcgagcag cgcaccaagt tcgatctcga aatgatccgt
    10561 gccatggggt attgccacgg aatcgagaac tattcgcgcc acttgagtgg tcgcgcgccg
    10621 ggcgatccgc ccccgaccct gttggattat tttcccaaag acttcctgtt gatcatcgac
    10681 gaatcgcacg cgtcggtgcc gcaggtcggc ggcatgtacg aaggcgactt ttcgcgcaaa
    10741 cggacgctcg tggattatgg attccggctg ccgtccgccg tcgataatcg gccgctgaag
    10801 tttgccgagt tcgagcggat gctgaagcaa gtgatctacg tctcggcgac gccgggtccc
    10861 tatgagctgg agcatgctaa gggtgaggtc atcgagcaga tcattcgccc gacgggactg
    10921 atggatcccc tgatcgatgt gcgatcggca aaggggcagg tcgacaatct gctggccgaa
    10981 gtgcggacgg aggcggcgaa gggaaatcgg gtgctggtga cgaccctcac caagcgaatg
    11041 gccgaagacc tcacggagta ttatcacgac ctcggcgtga aggtgcgtta tcttcactcc
    11101 gacattaaga cgctggaacg cgcggagatt attcgcgatc tccggcgcgg ggtgttcgat
    11161 gtgttggtcg ggatcaacct gttacgggaa gggctcgacc ttcccgaggt cagtttagtg
    11221 gcgatcttgg acgcggataa ggagggctac ctccggtcgc atcggtcgct gattcagacg
    11281 gcgggccgcg ccgctagaaa tctggatggg cgcgtgattt tctacggtga tacgatcacg
    11341 gattccatgc ggcgcgccat ggacgagacg gcgcgccggc gtcatatcca ggaagcctat
    11401 aatgaggccc atggcatcac ccccgagagc atcaagaagc agattcccct gctggactat
    11461 gcaacgggtg gcgggaatgc ggggcaactc gaactggcgg cggagtcagt aggcgattat
    11521 gcaacgacgg gcgacacgga gcagttgatt cgccgtcttg aggttgagat gaaggcggca
    11581 gccaagaaat tggagtttga gcgggcggcg gagttacgga atcggattcg aacgttgagg
    11641 ttgaaggcgt tggaattggc gcaatagtga aggcggctac tgtccctcct gcgccggaaa
    11701 ttctgttaca tctgtttgta gagataggcc ccgagaaaca tccccaacac gctgagcagg
    11761 ctcaccttga tgctgaagcc gaacgtcagt ttgaggagga ccagatcgac ggtgagtggc
    11821 gggttgattc cgggagagaa attgctggca aagatgtttt gaatggcgcc attcggggcc
    11881 atgacgcgaa gtatttcccc gaaaatcccg cccagcattc cgccgatcag gataaagatc
    11941 agcagcacgc cgatcgattt tctcatccga tatctctcct ggttcccgct ggtcggggtg
    12001 catgtgccga cttgaggcac catatcggaa gcggaaaatg atgtcaacaa aatgcatgcc
    12061 agaagcctct gtggatgcat gtggctgatt gcaaagcacc tcttctggcg atcggtggga
    12121 tcatagaggg caccctcggc gctcgagggt gaagcaggag aacgcccgac agtatttctg
    12181 gtgcaactaa gtctggcgcc gtgtgtcacg tgtatcaaca gtggtgcatt ggaacggtcc
    12241 gagggcatcg cttgaatccc tgccgaatat cttgacttca cgcacacccc ttctatagac
    12301 tgcccgatgc tattttttgt gatgtttttc gaggcgatcg gagctctggc tcgaccccgg
    12361 cttcgatggc ctttttgttt ggtgattcgt gcttgatgcg ttgagtgaaa agttcgaacg
    12421 aatcctcaag aagcttcgtg ggcagggtgt gctcaccgaa gacaatattg ctgaggcgtt
    12481 gaaggaagtc cgcctcgcgt tgcttgaggc ggacgtcaac tttaaggtcg tcaaggattt
    12541 ccttgaccgc gttcgcgaga aggcggtcgg gcaggaagtc ctgaaaagcc tgacccccgg
    12601 gcaccaggtc gtcaaagttg tctgggacga gctccgcggc atgatgggcg gtgagcggtc
    12661 cggtatcagt ctcgcctcca ggcccccgac cattattatg atggttgggt tgcagggagc
    12721 cgggaaaacg accacgtccg gcaaactcgc ccgcttgttt aagagccagg gcaagcgcgt
    12781 gttgctcgtg gcggccgacc cgcgtcgtcc tgccgcaggt gaccagttgg ccagcctcgg
    12841 gcatgatcta ggaatcgatg tgcatcgatt cgatcagatc gacgcctcac gcgccgatgt
    12901 ggtgcgtatc tgccagcgcg gggtggaacg cggtcaagag cagggctacg atctgattgt
    12961 gctcgatacc ggcggccggt tgcatgtcga tgatgagttg atggccgaac tcgtggcggt
    13021 caagcaggct gtgaaccccc acgaagtgct gctggttgcc gatgccatga ccggccagga
    13081 tgcggtgaat atggccggtc agttcgatca gcaggtcggc ctcaccggtg tcatcctgac
    13141 gaaagtcgag ggcgatgcgc gtggtggcgc cgtgctgtcg atccgggccg tgaccggcaa
    13201 gccgattaag tttctcggca tgggggaaaa gctcgatgcc ctggagccgt tccatcctga
    13261 ccggatggcg tcacggatcc tcgggatggg tgatgtgctt tcgttgatcg agaaggcgca
    13321 ggagacgttt tcacgggaag aggccgaagc ggcacaaaag aagctgacca gcagcacctt
    13381 tacgctggag gatttccggt cacaactcgg gcagatgaat cggttggggt cgttcgagca
    13441 gattctggga atgctgcccg gcggccaaaa gttgaaagac cttgcgaaca gcggattgcc
    13501 cgaaaaagaa atgaagcggg ttgcggccat gatcgactcc atgaccacgc gtgagcggcg
    13561 cgaccatacg ctgatcaacg ggagccggaa gaaacggatt gcgcgtggaa gcggaacgag
    13621 tgtgcaggag gtcaaccgcc tgatcaagca attcctgcag gccaggaaaa ttgccaaggt
    13681 catgtcgggt ggcgcgggag gccggcgtca gttggcccaa atgctccgag gcatgtagca
    13741 ggccacgagt gtttcgagtg tgcatcgatt cgaggtgaag aagagagtcc taaccgagcg
    13801 gacacagtct agggctgaac gctcattact ctgaggaggt tctagtggcg gttcatttac
    13861 gattggcacg gacagggcga cataagcggc cgatgtatcg ggttattgcg gccgattcac
    13921 ggaaaccgcg cgacgggcgc tttttggaga ttctcgggat tttcgatccg ctcaagaacc
    13981 cggcggtgcc ggtgttgaag tcggaacggg tgctgtcatg gttgcggcat ggcgcccagc
    14041 ccactgtgac cgttcggact ctgctgaagc ggcatggggt ctggaaagag ttcgagaccg
    14101 agaagtccgc aaaggcggag aagaccgaaa agaccgagaa gagcgcaaag agcaagaagt
    14161 agccggcatc acatggcgac gctcgatcaa gcagagctgg tgaccattgg aaggatcgag
    14221 cgatcgttcg gggttcgagg cgaggcgcgc gtgcgttccc tcacagatgt gcctggacgg
    14281 ttcgagtccc ttgggcaggt cacgattgtc gggcctgccg ggaagacgct cgacacccgt
    14341 gtgacccacg tgcggccagg cgggccgacg tggattatgg ggttcgaggc gtttacgacc
    14401 cctgagcagg tcgccgagtt tcgtggagcc ttcattcaag ttccccgcgg agactcgccg
    14461 gcgctgccgg ccgatcaata ctatcaatgt gatttgatcg ggatggtcgt gcaggatgaa
    14521 gccggaaccg tactgggctg cttggaagag gtcgtgtcta tgtccggcaa ccagaacttt
    14581 ctggtgcgtc acgacgggaa agaaatgctg attcctgcgg cgaaacaagt cgtggttgcg
    14641 gtcgacgtag tggggcgggt catgacggtg cggctgccgg aagggtttgg agatctgtaa
    14701 gatgcggtgt gccgttctga cattgtttcc ggatatggtg gctccggtgc ttggccagag
    14761 tatcctcaag cgcgcccagg aaaaggggtt gctggaggtc tctgtgcaga acctgcgtga
    14821 ccataccttc gaccggcata agaccgcgga tgatgtgccg tatggcgggg gagccggaat
    14881 ggtcatgaag gccgaaccgg tgcttcaggc tgtcgatgcg ctgcgggccc attatcaggc
    14941 ttctcatccg gagacgacgt tgcgggtgat tattccgtct ccgcaagggc ggccctttac
    15001 gcaggaactc gcacagtccc tggcgcagga tgaacgggta gtggtgtttg tgtgtgggca
    15061 ctatgagggg atggatgagc gcgtcaggct ggcgctgcac cccgaagaaa tctccgtcgg
    15121 cgattatgtg ttgaccggag gggagctgcc ggcgttggtg atgatcgatg cggcggcgcg
    15181 cctggtccct ggcgtgttgg gcgatgcggc gtcggcggcc gaggaatcat ttaccgaggg
    15241 attgttggag tatccgcact acaccagacc ggcggaggtg cgcgggatgg cggttccgga
    15301 ggtactggta tcgggtcacc atgaggcgat ccggttgtgg cgccggaagg aagcgttgcg
    15361 gaacacctat ctgaagcggc cggatctcct gcgggatcgc gagcttggat cggaagatcg
    15421 acggttgtta agcgaagtga tgcaagagag tcttgtacag gtacctgttc gttgagagga
    15481 ggagaaggac tatgaatcgg ctagaacgga tccaacgatc cttaaccaag aagacgacgc
    15541 ccaagttcga gatcggggat actgttcgtg tccacgtgaa ggtggtcgag ggtgaaaaag
    15601 agcgtatcca ggtgtatgag gggacggtca tcgccaggaa aggcacgctc aatagcgaaa
    15661 ccttcactgt gcgcaagctg tcctacaacg tcggcgttga gcggaccttc cccatccact
    15721 cgcccaatgt ggccaaggtt gatgtcgtcc gtcagggtcg tgttcgccgg gccaagctct
    15781 attatttgcg caccaagaag ggcaagtttg ccaaggtgga agatcgcgag tttaaggtcg
    15841 agagcaaggc gcaaaccgcg gccaagcgtg aggcggcagc agcagtcacg gctgctaagg
    15901 cctgatcgat cggttgtgct gtgttccgct tgagaccagg acagaggcgc acgtttggct
    15961 ggttcttcga acccgacgac agggggcccc accgaggagt ttgaggtgga ggctcggtgt
    16021 cgcgggtacc ggcatatagc cgggctcgat gaggccgggc gtgggcctct cgccggtcct
    16081 gtcgtagcgg ctgccgtgct gttgccgcgt cgatgccgcc tgcccggact caacgattcg
    16141 aagcaggtcg gcgagtcaga ccgagttcga ttgttcgccg aaatcgttcg ccgggcaacc
    16201 ggcgtcggca tcggagctgc gacggaggcg gagatcgatc ggttgaatat ccttcaagca
    16261 acccggttgg cgatgcggcg tgctctgcag gcattaccgc ttcaaccgga ctttttgttg
    16321 ttggatgcgg tcactctttc cgggctctca gttcctcaac gttctatcat caagggcgat
    16381 ggcctctcct gctccattgc ggccgcctcg atcatcgcca aggtgacgcg cgaccgtttg
    16441 atggttgagt accaccgttg gtatccccaa tataattttg ccgagcataa aggttacggg
    16501 actccggagc atctccgtct gctccgggag cacgggccct gtgcgattca tcgccggagt
    16561 tttgcgtctg tgcagcagct ctgtgtcggc gatgctcgaa aattgtgttc ctctgtcgac
    16621 cagtctgagg gcccgctgga ccgatgcgcg accgacgccg attggtaggg gatgaaggag
    16681 aggggcgagc cgaagcctat ctgcgccgac aggggtttcg aatccttgga cggaatgtac
    16741 gatccccatt gggtgaactc gacctggttg ccgatgacca gggggtgctg gtgttcgtcg
    16801 aggtcaaacg tcgtcggacc ggaacctacg gcggtgcgat tgaagcggtg gatgcgcgca
    16861 agcgagccaa attgattcag cttgcggcgc agtatctggc gcaacaccgg attcatgatc
    16921 gtgcgtgccg atttgatgtc gtcttgatac aggatgattc cgggacgact gaacccgtgc
    16981 agcatattgc gaatgcattc gacgtcgcgg gggatgatct gcgatggtag gatgggaatc
    17041 gcgctgctga ttcatccagt cactgtgtgt cgtgccgggc ggcgggggcc cgagaggtca
    17101 ctccggtccg gcggctgctc ttgtcaccat ccatgattca actttttcac gtctcaaaat
    17161 attacgaccg ccgtttggcg ctgtccgatg tgacccttga aatcgagaag ggcgagttcg
    17221 tgctcttgat gggaccgagc ggcgccggaa aatcgacctt gttgaaattg ctgatcggcg
    17281 cagagcgccc tgaggaagga cagatcctgg tgcagggccg tagtttgtcg aaattgcgat
    17341 cgtctgaaat tcccttactg cgtcgaaaga tcggtgtggt gctgcaggat ttccgcctgc
    17401 tgccaaagaa aaccgtattc gacaatgtgt cgttgccctt gttggtgcag ggggcctcga
    17461 ctgtcgaaat caggcggaaa gtgacggacg cattacggtc ggttgggctg gatcacaaga
    17521 aggatctctt tccccccggc ctctctaccg gtgagcagca gcggatttgc atcgctcgcg
    17581 cgatcgtcaa cgggccgatc atgctgttgg ccgatgaacc gacggggaat ctggatccgg
    17641 agctgaccag cgaaatcatc gaactgttca agtcgatcaa cgcccggggc acgaccatca
    17701 tcgtggcgac gcacgatccc aatgtcttgg cccaggtcaa tcgccgggtg atcacattgg
    17761 accagggcaa ggtggtctcg caggaacagg tgtgctcgtg aggcgtgtgt tgtacctgct
    17821 gcgggaagcg gtcgccaatg tgttgaccaa tcgaacgacc acggtggtcg cggtggccac
    17881 cacggccttc acgtttgcct gcgtcggcgt gtttttgttg ttgtatgtca atttgaggac
    17941 catggcctcg tcgcttgagc aggacatcca ggttatggtg tacctgcagg acgacctgac
    18001 ggagcagacg agaaacgaaa tcgaactgca actgaaggcc gatcgtgccg tcgggtcgtt
    18061 gaccttcgtg tccaaggaac gggcgttggc tgatttccag agtcagtttc cggccgagtc
    18121 ccatctgctg caggggctcg ggcagaaccc gctgccggcc tcatttgtcg tgacgcttgc
    18181 ggtggagtcc cgttcatcgg atgcgatgcg gcggtgggcc aatcgcaccc agtcgatccc
    18241 gggagttgcg caggtgcaat acaatcagga atgggtcgag gccttggccg gcattgtccg
    18301 gtatatcgag gtggctgcca tcattgtcgg cctcatttta tcggcggcgt cggtcacgat
    18361 catcgcgaac acaatccgcc tggcgctgta ttcacgccgg gaggaaattg aaattctggg
    18421 gttgatcggg gcgagtacga cctttatccg tgtgccctat ttgctcgaag gtgcggcgct
    18481 cgggttgctc ggcagtgctc tctcgctggt aatcctcaaa gccgggtttg agttgttccg
    18541 ccatgaaatc cggtctgcca gccggtttct gggcgtggac gcgctgctga catttttccc
    18601 cttccaaatg tgtgtggtgc ttgtctgtgt ggggctcttt ctcggttgtg ccggcagttt
    18661 tctctcgctg ctccgattcg gagagggacg ggcatgaagt gggcgcgatg gggatgtgtt
    18721 gtggtggctg ctcatctggt cttgtggact gtccctgcct gggcggagcg caaggactcg
    18781 tacgcggaca agatcgaaca agaaaagaaa acgctggaaa agttgcgcgg cacgattgtc
    18841 gagaaacgta agaaggcgga cgaggcggaa aagaaacgcg agtcggtcct gcagggattg
    18901 cagtccttgg acgaacgtct ggttcgttac cggcaggaac accaggacgt cgtgaagaag
    18961 ctcaaaaaga aggatgtgga aatcgagcag atgagcgtgc agctgagccg gctgtccgaa
    19021 cgcatcgatg aacggcagga tgccatcgct gctcgcttgc gggttcaata tgtcgagggg
    19081 cggttttggc acctgagaac gctcctggcg gccagttctt ccggagactt tcagcgacgg
    19141 tttcgatatc tctcggccgt gacgcagcga gagtatgaga tcatggaaac ctaccggaaa
    19201 gatgccgagc ggatcgccga ggtcgagcgg agtcgagaag aggcgcgcca gggcatcctg
    19261 gcctacaaag ccagcaccga ggaaaagctg gctcagatcc aaggactgaa aaaacagaaa
    19321 cgggtgtatc tcgctaaaat cactcaagaa aaggaatcgc atgatcgggc cgtagaggaa
    19381 ctggagcggt ctgccaaccg ggtcgacagc ttgctgaagg aactggaaac cagacgtcgg
    19441 gcagcgctgg cgactcgtcc gccctccgtg acaggaggcc ttcgcgcatt acgtgggacg
    19501 ctcctctggc cgaccgaagg gcaagtggtg tcctacttcg gccggcagaa gcatccgacc
    19561 ttcaatacct acatccagcg aaaagggatt gagattcgcg ctgcggaagg gagtaatata
    19621 cggtcggtgc ttgccggcca ggttgtctac gcggactggt tgaaaggcta cggactcgtt
    19681 ataatcatgg atcatgcaaa cggagtcttt tcgttgtatg cccatgcgtc gaaaatattg
    19741 acgtcggtcg gggcccggat cgaagccgga gaagcgatcg gtgaaacggg cgacacgggc
    19801 atgacgggtg agaatacgct ctattttgag ttgcgcgagg gagcggaacc ggtggatccc
    19861 ctcgtgtggc tgtcgaagcg ataggcctcg gaaggaagga aacctacgtt atggaacagc
    19921 ggcgaagccg acggtggtta tatcttttgc tgatggtgac ggtagccctg ggtatcggca
    19981 ttgtgctgga aaagggattg gagcggaccg gccacgcctc cgagacctat gaagagctgc
    20041 ggaccttctc cgaggtcttg acccaggtgc agaagcacta cgtcgacgat acaaaggtga
    20101 aagacctcgt acagggtgcg attcgcggga tgctctccac gctggatccg cactcggcct
    20161 atatgacacc tgagatgtac aaagaaatgc aggtcgaaac gaaaggcgaa ttcggtgggg
    20221 tcgggattca gatcggtgtg aaggagaacc gcctcgccgt catctccccg atcgaaggca
    20281 cgcctgcgca tcgagccggg atcaaggccg gtgatttcat taccaaggtg aacgacgaac
    20341 ccacaaagga tttgacgctg atggatgcgg tgcagaaaat gcgcggcccc aaaggcacca
    20401 aggtgaacct cacgatccag cgggacggca cggcggaccc gctggcgttt tcattggtgc
    20461 gggacaccat caagatcgaa agcgtgaagt tcaaggtgct cgacaatacc attgggtatg
    20521 tgcgtttgac gcagttccag gaagccacgg gacgggacct cagccgggcg ctcaagcagt
    20581 tcaaggagca gaaggttcag gggacgattc tcgacttgcg gaacaatccc ggtggcctgt
    20641 tgacggcggc cgtcgatgtg tccgaacagt ttgtggggaa cgggaagctg atcgtctata
    20701 ccaagggtcg tgaggggaag aaggatgagt ggttctccaa gacgaaggag acgctggaag
    20761 attcgccgat gatcatcctg gtgaacgaag ggtcggctag cgcgtcggag attgtggccg
    20821 gtgcgttgca ggattgggga cgggccgtca ttgtcgggac cacgtcgttc ggcaagggat
    20881 ccgtccagac gattctgccg ctgggtgacg ggtcgggact ccggctcacg actgccaagt
    20941 actacacgcc gaaggggcgc tcgattcagt ccaccggcat cacgccggac attgtggtca
    21001 aactgcaaac gcagaccgtc gcaaaggccg gcgagaaaga cggaaaagag tctgaaccga
    21061 agacggccaa agcgcctgca gccggaaagg atccatcgcc ttcggccaag ccgggtgagg
    21121 agcctgcgca taagaatggc gccgtgtcgg ccggggatgg ggccggggat atttcgctgg
    21181 atgatgacgt gcagcttcaa aaggccgttg agctgttgaa gacctggaag atctttaagg
    21241 agctgcggcc ggcggcgtag tcggcacggt tccgatccgt tcgcggatgg cggcgagcag
    21301 cgtgtcttcc gacccggaaa aacctggttg tgttcgtaag agatgggacg caaccaggct
    21361 ttccaggtcg gccaatgtgc ggatgttcag ccggaaatat aagtaactca ccagggcatc
    21421 ggagaggccc ggcaacgaag cccagccggc cacttcctcc ggcaacggtg tcttcagttc
    21481 ttcataggcg cgaatcgttc ctgtcgccag aaactcttca atcttcactg ccaagtcccg
    21541 cccgattccg gggatctcct gaagctgatg gcgcgaggct agatcagcca ggtctcccgt
    21601 aaccgtcagg atcgaatcag ccgctttccg atacgcgcgg acgcgataag gattggcacg
    21661 ttggcttgcc aacaggttgg ccatcgcacg aaagatctgc gcaagttgat gggtgcggtc
    21721 gtccatagtc gtgtgcccgg cgctctttat ctacattgtg cgagtatcga atcccaaacg
    21781 gcaaggccta accgggcttg gttgacaacc ggttttcccg tttcatagga tgatctgaag
    21841 aattgaggtg cgcagtgatc attcacgtga atggtgaatc tcgcggaatc ggcgatgggg
    21901 agacggttgc agcgctgttg cacgagttgg agatccgcgc ggatcgcgtc gccgtcgaat
    21961 tgaatttgga gattctggat cgcaaggatt ttgaaacccg cggactccgc gagggggatc
    22021 gggtggagat attgagcttt attggtggcg gagcgaggta accacattta tggatacgat
    22081 caacgatcgc ttagtgattg ccggccggga atttacgtct cgcctctggg tggggaccgg
    22141 taagtataaa gactttgtcg agactaagaa ggctatcgag gcgtccggtg cggacgtggt
    22201 gacggtggcg gttcgtcgcg tgaacatcac ggatcggtcc aaggaaaatc tcctggatta
    22261 tctcgacccg aaaaaataca tcattttgcc gaacaccgcc ggttgttaca cggtggaaga
    22321 tgccgtccgg tatgcccggt tggctcgcgc ggccggcgtg tccgatctgg tcaagctgga
    22381 agtcctgggt gatgaaaaga ctcttttccc ggacaccgcg ggattgatcg aggcggccaa
    22441 gatcctcatc aaagaaggct tcatcgtcct gccctatacg aacgatgacc cgatcgttgc
    22501 caagaagctg gtggatatcg gctgtcccgc ggtgatgccg ctcgctgctc cgatcggctc
    22561 ggggctcggg atccgtaatc catacaatct caagatcatc atggaaacgg tcaaggtgcc
    22621 catcatcgtg gacgccggag tcggaacggc atccgatgcc gcgctggcga tggagtacgg
    22681 agcggatgcg gtgctgatga acaccgctat tgccggcgcg caggacccca tcgcgatggc
    22741 cgaggccatg aagtatggtg tctgggccgg gcgactggcc tacaaggccg ggcgcattcc
    22801 gcgcaagctc tatgcgacag ccagtagccc gatcgaaggc atgctctaat agccagagaa
    22861 ccgtctttgg tcggtgtcgg cgagccgggc tgagtgcgtg tgtgagtggt gaaccggtcc
    22921 tgctctcccg gcatctcaca ttcaactggc atcattcagc atccagaata tttcccgaat
    22981 gccctccgtc gatttccgtc tgtatttagt gactgatcgt caacagaccg ccggacgtcc
    23041 gctggtgtcg gtcgtcgggc gggcggtgag tgccggtgtg cgcgcggtgc agctgcgtga
    23101 acgtgatctt ccgatccgag agctgctctc tttggcgctc gacctgcagc gcgagttgtc
    23161 ggacatgcag gtgttcatca atgaccgtgt ggatctggcg gtggcactgg ggtgccgggg
    23221 ggcgcatttg cgcgagagca gtttgccggc cccggtggtg cgaacgctgt tgcagccctc
    23281 gcagctgttg ggtctgtccg tccattccat ccaggatgcg gtggcggcag aacggcacgg
    23341 cgcggatttt gtggtgttgg gtccgattta cgacacgcct tcgaagcggg agtatggggc
    23401 gccgcttggc ctccaggtac tcgaacaggc cgcgcgcgcg gtgaacattc caatttttgc
    23461 catcggaggg atgactgcct cgcgtacacg tgaggtgcgg caggcgggag cattcggtgt
    23521 ggcggtgttg tcctcgatct tgagtgcgtc gaatgtcgaa gtagcgaccg agaaatttct
    23581 ttcggcgatc gagcgcgaat catgaagagg attgctgcag agggcgtctc acgcatcgac
    23641 acacgtcacg ctgcatcggc gtctgctgct gttgaccacc ccataggcgg gtgatagaat
    23701 ccgaaacagt gttgatgacc gttactgaca gggattgtaa gcgatccaac gagtggggcg
    23761 atggccgaat caaaagggag ttcaggccga ctccggtcct tccgtgattc cgtcaagcgc
    23821 ctgaccaagt cactttctga cggagatgga gtcgtgacac acaagaacga cgaacaggcc
    23881 cgcgaggtag aaaaacttcg catccaaatc cagtccatgg aggaggagat tcggcggctc
    23941 tatcagtcac gctaccagct cgaacaaacg acgaagcaga acgaaaagct cgtcgccact
    24001 ctccaggaag ccaaatctca aatcgaaaca ttgcgggctg aagtggagaa gttgaccgct
    24061 ccgccctcga cctacggcat tttctccagc ctcgataccg atggcacggg gaatgtctac
    24121 gtatccggcc gcaagatgaa ggtcagtctt catccctcca tcaaagcgaa gtctctccgc
    24181 aaagggcagg aggtcatcct caacgaagcg ctcaatgtca tcgaggtgcg tgggttcgat
    24241 gtgcaagggg aagtcgtccg tttgaaggat gtgctggagg ggaatcgggc gctggtgacg
    24301 ctgcacttcg atgaagaaaa agtcgcggag cttggcgagc cattgctcag tgagcgcctc
    24361 agcgtcggcg atcatctgtt atacgatccg cggtccggtt gcgtcatcga aaagctgccg
    24421 aaatcggaag ccgaggagct ggtgctcgaa gaagtccccg atgtggacta cgaacatatc
    24481 ggcggactcc agaaagaaat cgagcaggtg cgtgatgcgg tggagttgcc tttcctgtat
    24541 ccgcacattt ttgcgaacta caaactcagc gccccgaagg gcgtgttgct ctatggcccg
    24601 ccgggttgcg gcaagacgtt gatcgccaag gccgtggcca gttcgatcgc caaaaaactg
    24661 gggcatctca aggacaaaca actgcgcagc tacttcctcc atgtgaaggg gcctgaactg
    24721 ttgaataagt atgtcggcga gtcggagcgg caggtgcgcg aggtgttcaa aaaagccaag
    24781 gaacgcgccg acgacggtca tcccgtgatt gtgtttttcg acgagatgga cgcactgttc
    24841 cgcacccgcg gcaccggcat ctcctcggac attgaatcga ccatcgtgcc gcagttcctc
    24901 tcggagatcg acggcgtaga gcgtcttacc aatgtcatcg tcatcggggc aagcaaccga
    24961 caggacttga tcgatccggc cgtgctccgc gcgggtcgtc tcgatgtgaa ggtgaaggtc
    25021 ggtcgtccgg acgccacggc ggcccgggat attttctcca agtacgtctc gaccgatttg
    25081 ccgtttgccg aggacgatct gaagcggcat ggcggggatg cccgggcact tgtggattcc
    25141 ctcatgaaca tgaccgtcga tgccatgtat gcgacgacgg acgaaaacaa gttcattgaa
    25201 gtgacgtatg cgaacggcga gaaggaagtg ttgtacttca aggatttcgc cagcggcgcg
    25261 ttgatcgagg gcatcgtgtc gcgtgccaag aaattcgccg tgaagcgggc gattgcgcaa
    25321 gaaggaaccg gtctgcgggc cgaggatctg atccgcgcga tccgagagga gttcaaagag
    25381 cacgaagact tgcccaacac cacgaatccg gatgattggg caaaggtcgc cggcaagaaa
    25441 ggcgagaaga ttgtccacct ccggacgatc agcggcgggc ctgcagaggc gcgccaaatc
    25501 gaaacggtca acaccggtca ctacctgtag cgcgatattt catgagcgac aatcctacgc
    25561 acaatccgtc ccgtgtcatc ggcacggaga ccgaattcgg tatcgccagt cgtgacccga
    25621 atgcggccga tccggtggcc aactccattc acctcatcgg ctactacccg aatcttccgg
    25681 ctccgcatgc ggtgtgggac tacgagaacg aaaatcccct gctggatgcg cgcgggtttg
    25741 aggtcgatgg tgagcgggaa cggccgggac ctgattacaa tcggcaactc aataaagtcc
    25801 tggcgaacgg tggtcgtctc tatgtcgatg gcgcgcatcc tgaatattcc acgcctgagt
    25861 gtctcaatgc gagggaagtc gtggcgttcg agcgcgtcgg tgaacgcatt gtcgcgcagg
    25921 ccctggaagg gattaccaag gcgcgcgggc gtgaccagtt cgtgttgtat aagaataatt
    25981 cagacggtaa gggcaacagc tatgggtacc acgagaatta tctcgtgtcg cgggcggtgc
    26041 catttgaacg catcacccag gtcctcacgc cgttctttgt gacgcggtcg atttatgccg
    26101 ggtccggcaa ggtcggcgcg gaaaaccaga ccagccccgt cgactatcag atttcgcagc
    26161 gggcggattt tttcgaaacg ctcgtggatc tgaacacgat ggttcggcgg ccgatcatca
    26221 atacgcgcga cgaaccgcat tctgattcgg cgaagtaccg gcggctccat gtgatcgtcg
    26281 gagacgccaa tatggccgag gtatcgacct atctgaaggt cgggaccctc tcgattgtgt
    26341 tggagttgct cgaggccggc gccgaccttc ctcgaatcag cctggcagat ccggtgaatg
    26401 ccatcaagca ggtctcccgg gatgtgcagg tgaaggagag tgtgaagctc agtgacggga
    26461 gctcatcgac tgcgatcgcc gtgcagcgag cgtacctcaa ggccgcccag agctactatg
    26521 cctgccatga actcaaccag gtgacgaagg acgtgctggt gcggtgggaa gatgtgcttg
    26581 atcgcctgga acgggaccct cggtcattgg tgcgggagtt ggattgggtc gcgaagcggt
    26641 acttgatcga atcctatatg gaacgcaaat cctgtggctg ggacgatccg cgcgtccgcc
    26701 tgatggattt ccaataccat gatgtccgtc ccgacaaggg cctgtattat accctggaac
    26761 ggaatcatcg aattgagcgg gtggtattgg atcatgagat tgcgcgggcg gaattcaatc
    26821 cacctgttgg gaccagggcc tatttcaggg gccagtgcat caagaagtac ccgactgtcg
    26881 tctacggggc aagttggacg tccgtgctgt tcgataccgg acaaaacaag atcaagagaa
    26941 tccccttgat ggatcccctg cggggtaccg aatcgctcac gggggaactc ctggcgcaag
    27001 cggagacggc gtccgcgctg ctcgctaaac tttcgacctg actgtgacca ttgaatgaaa
    27061 cattccttct tcttttcaca tcacgagggg tccagcttct tcgacttcct cacccagcac
    27121 catcctgagt taagacccgg tttgagtgag atcgccatcc agcgagccgc gcttcccgtg
    27181 gatttcggcc gacccggaac gatcccggtg ccgcatggga cgacggttct cgcgttgaaa
    27241 tatcgggatg gggccattat tgccggtgat cgacgcgcga ccgagggatt tcagattgcc
    27301 gaccggcgga ttgaaaaggt ctttcgcatc gacgactatt ccgcgatggc gatcgccggg
    27361 gctgcggggc cgtgcatcga gatggccaaa ttgtttcaga cggagcttga gcattacgag
    27421 aagctcgaag gcgtgcagct gtcctgtgag gggaaggcca acaagctggg gcagatggtg
    27481 aaggccaacc tcccgatggt atttcaggga ctcgtcgtca tgccgcttta cgtcggatac
    27541 gatctcaagc ggaacgaagg gcgcattttt aagtacgaca ttacaggggg acgctacgag
    27601 gagtccgacc accattccat cggctccggc ggcaaggatg cccgcaacac gatgcgtgaa
    27661 cattaccgat ccggcctcca ggaagaagag gcgctgcggg tcgggctgtt ggccctctat
    27721 aatgcggcgg atgaggatgt gggtaccggt gggcctgatt tagtccgcgg tatttatccg
    27781 acggcgaaaa tcgtcagtgg cgccggtatc accgatgtgc ccgaagatcg ggtgcgtgcc
    27841 ttgttcgaaa ccatgatcgc agagcgaagg agatcctagc cgatgccgtt gccctactac
    27901 gtctcgcctg aacaaatgat gcaggacaag gcggagtatg ccaaaaaagg cattgccaaa
    27961 ggacgctcca tcattgcgct tgaatatatc gacggcatcc tgctcgcagc ggacaatcca
    28021 agctcctcat tgcataaagt gtcagaggtc tacgaccgga tcgcgtttgc cggagccgga
    28081 aaatatagcg agttcgagca tctccggaag gccggcattc gccatgccga tctgacaggg
    28141 tacatgtaca gccgggaaga tgtgagcgcg cggactttgt ccaatgccta ttcgcagagt
    28201 ctggggacgg ccttcagcac ggacgtcaag ccgctggaag tcgaaatcct tgtcgtgcag
    28261 gtcggtgtga acggtcaggc gaatgagatc ttccgtattt cattcgacgg cagcatcgtg
    28321 gatgaaaaaa atctcgcggt gatcggggga cggtcggaag ccgttcagca atatttgcgg
    28381 gaacatgcga caccgcagcc acccacgttg aaggatgggc tccggcgttg ccttgcctcc
    28441 cttgagcagg tcgccactca aaagatccct tccgaaaatc ttgaagtcgc cgtgctggac
    28501 cggaaccgcc ttgggcggaa gttcaaacgg ttgagcagtg ccgacatctc ccagctcttc
    28561 gcctgacatc ccctccacgt aactgcgtgc ttatccgctt tccggtcggt gagacgcttt
    28621 tcattgccgg gcatctgttg aagcctcacc cgcagcggtg tatcctgaga gaaacggatt
    28681 cgaggaaacg tctctccatg cagcaacgta tcttcggttt agaaaacgag tacggcctaa
    28741 ttttctctcc gaacgggaag atttatcttc cgatggagaa agtgctgggc tacatcttcg
    28801 aaggcctcat tccgaacagc tggccgtcga atgcattcct ggtcaacggc gcgcgctttt
    28861 atcaggatac cggctgccat ccggagtact ccacgccgga gtgcgacaat atcctcgatc
    28921 tcgtcatcca cgataaagcg ggcgagcgct tgttggaagc ctgtttgccg gcggctgaag
    28981 agcgcctgcg cgaagagggg ttgtccggag aaatttatat tttcaagaac aacaccgatt
    29041 cgctcggcaa tacctacggg tgccatgaaa attatctcat gcgccgggac gtggatttct
    29101 ggaaagtcac cgagcagctc attccgtttt ttgtgacccg gcagatctat tccggcgccg
    29161 gtaaggtcct gaaagtctca ggtaaaccgc agtacttcat ctcgcagcgc gcccagcata
    29221 ttcacgaaaa gacctcctcc tcgacgacct cgtcccgaag tatcatcaac acccgtgacg
    29281 agccccacgc cgatgcggag aagtatcggc ggctgcacat cattgtgggc gactccaata
    29341 tgtcggagta tgcgacgtat ctgaaggtcg gcaccgccac gttggtgctc tcgatgatcg
    29401 aagccgggta ctcggtcagc gggatggaac tcgaggatcc tgtgaaagcc atccgtgaaa
    29461 tttcccgcga tccgaccctc aataagaagg tcaagctgga tgacggtcgg cagatgactg
    29521 ccatcgagat ccagcgggtc tacgtcaagc gcgcgaaaga gtttctgtcg gagcaggaac
    29581 acgatccggt gctggacgac gtctgggaga agtgggtgtc ggtgttggat cgtctggaag
    29641 aggatccgat gcaactcgtg cacgaggtcg attgggtgat gaaaaagcat ctgattcagt
    29701 cgtacaccga caagaaggat tgcggctggg atgacccccg ggtgttcctg ttggatttgc
    29761 aatttcatga cgtgaaacgc acgcgggggc tgtattatct gatggagaat cgagggatgg
    29821 ccgcgcgggt cgtggaggag gaagcggtgc aacgggcgat gtccgtgccg cctcagacca
    29881 cgcgcgcaaa agtccgcggc gactttatcc ggttcgcgcg ggccaagaac cggtcgtaca
    29941 cggtcgactg gacctatttg aagttgaacg ggtactggga agagaccatt ctctgtatgg
    30001 atcctttcag cgcggtgaac cggcgggtcg aagaattgtt atcgcaggtg tccggcgcgc
    30061 gactatatcg atgacagagg ggcacgggca gagacgagac aagaccatgt cgttcctgga
    30121 tcgttttctc atcatttcgg tcgtgctgct gacgagtgtg tttcccgtcg aactctttcc
    30181 gaacacgggg ccggcgcctc gtggcgggga cgggcaattc agcggcaagc agggccaggt
    30241 gatcctcatc aacgtgccgg acatcaagga tgcggccagc gtcaaaggtc agtttcttgg
    30301 gcgtaaagtg acgctctttc cagatccttc tgccggtggc ggtgccggtt atgtcggatt
    30361 gctggggatc gacctacagg atgagccggg tgcgcatgag ctcacgatcg atgcccaact
    30421 gggtgagcag acgcgccatt tttcctatca ggtgttggtg ctgaaggaga agttcgccgt
    30481 cgagcatctg aagttgccga aagacaaagt cgatctcgac gagaaagcgg ctgcgcgctg
    30541 gaaggccgag caggagcagg ttcgcaaggc gttggctgaa gagtctgcca tgcggctctg
    30601 gcaggccggg ttcatcgaac cggtccatgg taagcggacc gggatttttg gcagtgtccg
    30661 catcatgaac ggtcaggcca gaaacccgca caacggagaa gatatcggcg cgccgatggg
    30721 caccgacgtg atggccagca atgacggtgt cgtccggctc attgtggacc atatcttttc
    30781 aggccgcggc atctttgtgg accatggtct cggcctgtac tcgatgtact ttcacctctc
    30841 cgacgtgctg gtgaaagaag gtgatttgat caaggccggg cagatcatcg gcaaagtcgg
    30901 cgccaccggt cgcgccacgg gtccgcatct ccattggggc atgaaggtca acggggcgcg
    30961 cgtgaatccc tacgctctgc tcgaattgcc ctttccccag aaccccgctg ttgcacggcc
    31021 tgtgttggcc tccccgacgc gtcccgcaca gggcgaatca agcgccaccg cagtcggcgg
    31081 tgatacgcga taacgcgact cgctgccgac ctgccgctcc atctgatggc ggatcaattc
    31141 tcaggatccg tgagccgttt ccctcaggtc tgattcgaca acgttggatc aagcgcggcg
    31201 ttcactgcct ttatcagcga gtcgggggta tagggcttgg gcagaaatgt ggtccgtgca
    31261 tcaagctgtt ccagcatcga tccttcgcgg tctgaatagc cggaggtgag gatgatgttg
    31321 atctccggtt gcagcgtccg gagttcggcg gcgagttgta tcccccccat gccgggcatc
    31381 accatatcgg taatcacgag tgagattttc cctccccgct ggcgaaccag ctggagcgct
    31441 tcctcgccgg ttcgcgcggc cagtaccgaa tgatgctgtc cgaccagcac cgtccgtgcc
    31501 aggcctcgaa ccatgtcatt gtcttcgacg aggagaatag ttttcgactg ttcaatgatg
    31561 ctcctgggtt gagccacagg gcgcggcgat tcggaatcct cgtcgatgca cggcagataa
    31621 atcgtaaatg tggtgccacg tccgggctcg ctctcaatcc caaccgtccc tccgctctgc
    31681 cggacgattc catagaccgt ggacaatccg aggccggtac ctttgcccag ctccttcgtg
    31741 gtgaaaaacg gctcaaacac atgagcgacc gtgtcggcat ccatcccgca tcctgtatcc
    31801 cggacggcga gcgtgacgta cgggcccggg gtgaggggat caggccagag cggatcggag
    31861 tgagtgatcg agaccgattt cgtctccagg tccaggcggc ctccctgcgg catcgcatcg
    31921 cgcgcgttaa ccaccaaatt catcacgacc tgctcgattt ggccgggatc cgccttgatt
    31981 cgtccaagtg caggatccaa agtgagggtg agttgaatgt cttccccgat gacacgtttc
    32041 atgagctcga caatgtcggt cacgcggtca ttcaggttga gcggttggcg ttccacaatc
    32101 tggtgacgtg tgaaggccaa caactgccgg gtgagggcag tggctttatt gccggcttcc
    32161 ctgatttgtg ccatcccgcg ctgcgtccgg gtggtttgtc ccggctcatc cgtcaagagc
    32221 tcagcccagc tattgataat cgtcaaaaga ttgttgaagt cgtgtgcaat tcctccggcc
    32281 atgcgtccca gggcttccat tttttgcgcc tgacgaagct ggcgctcgag atcctttcgg
    32341 tgaagaaatt gagtaatcat ggcccccaac tcggaagccc gttccaatcg agatgagctg
    32401 ggccgcagcc gttcgtaggt gagaaactcc atgatcccca ggatctcttt tcccgtgtga
    32461 atggggaacg cgatccctcc gtggaggtcg gctttttgcg cagcgtgaag gatcgggcag
    32521 tcggcctcgt tgagaatatc cgggcagaga gtggcatccc ctgtcttcca gacctggccc
    32581 gccaaaccgg ctccctgccg cagggcaagg ccccgataga tccgggcgaa gttctcggca
    32641 gggaggcttg gttggtgcca cagggcattg cagcgcaagg tcttgtcttg tgagtgtacc
    32701 agccacagtg cgcccagcgt ccagtcttgg agttcgcaca tggtgcgaag aatttttggt
    32761 accgcttcac cgagagaggc ggattcctca agggcgaggc cgaccgcgag ttgaacggca
    32821 tgcatcgttt cagcttgccg tcgttcggtg atatcggcct tggcgccgat catgcggtag
    32881 gggcggccct cgtggtcacg gagcacatac ccgtgatcga tgacataggc aaaggtgccg
    32941 tcggctcgac gaaatcgata ttcggctgtc cacagtttct ggtcggactg aagcacttga
    33001 gcgaggttgg tgagaaccgc ggtctggtcc tcagaatgaa tctgctcggc ccagggaatg
    33061 tggcctcgcc gaagcttctc aggggcggaa ccatataccg attggtgcgc ctcgttgaac
    33121 cacgttacgc cggaggtgat ctcccagtcc cagatgccgt cgcgggtggc gaggcaggtc
    33181 agatgaaacc gctcctcgct cttccgtaag gcttgctcga cgcggtgccg gcccatggca
    33241 taacgaatgg cgcgtcggag tgcgtccccc gtcagccggc ccttcacgag atagtcctgc
    33301 gcgccatgca ggagggattt ctcagccacc acgtcgttat ccagccctgt caacacgatg
    33361 accggcgcgt catgtgcttg agcccgtacc cgatcgagcg tctccaggcc ttggctgtcc
    33421 ggcagcgaaa gatcgaccag gacggcatcc accgggcatt ccgccagttt ttttatgccc
    33481 gtttggagtc tgtccgccca gttgagcgtg acagcgtccg tcgaccgttc gcagagcgcc
    33541 tgttggatca gctcggcatc atcttcgttg tcctcgatga gcaacacatg catgtgtcac
    33601 ctcttcagcg tcgggactcg cgagaccagc gtccaataga gttcgaactc tttcacgacc
    33661 gacaggaacc ggctgaaggt gacaggcttt cgaatgtacg agcaggcgcc ctgttcaaac
    33721 gaccgcacga tatcctcctc gcgctcactg acggtcagca tgacgaccgg gagcggacga
    33781 aagcgggggt cggacttaat ttcaatcagc acctcgagtc cggttttctt cggcatgttg
    33841 atatcgagga gcaccaggcc ggggaacgga cattggctga aaggaccttc accccgcaaa
    33901 taggccagcg cttcttcgcc gtcccgtacg acggccactc gattagccat gttcacggtc
    33961 tcgaaggctt cccggatcag caagatgtcg tcttcattgt cttctgcgat aagaatatca
    34021 aagggccgca tggtcatcgt tgcaccttcc tcggactaga ctggttgacg ccgaatgtaa
    34081 tgaaaaattc cgatccgcca ccttcgcggg gctccaccca ggcacggcca ctgtgtcgtt
    34141 cggccacttg ccggacgatg gccagtcctg cgccggtccc ctctatctcg cgtccgaccg
    34201 cccgtcggaa gagttcgaat atccgatcgc gttgctcagg agccactccc gggccacggt
    34261 cgcgcacgac caggccgatg agttcgtttc cttcaagatc catcccggaa tgtggcgcaa
    34321 tctcaatgtc aggagcttgc ccggcggatg caaacttgag cgcattcgcg accagattat
    34381 agataccctg gatggcccag gtggtattga cccgcaggcg gggaaggggg gagcgaatcg
    34441 tgattctggc gtcggtttct ttgatgcgct tctccagccg acgtaggact tcgtgcacca
    34501 gctgttccgc gtcgatgtcg tcgacgggcg gatccatgcg ctg
//
LOCUS       DAOQRM010000010        34478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_677_length_34478_cov_14.105337, whole genome shotgun sequence.
ACCESSION   DAOQRM010000010 DAOQRM010000000
VERSION     DAOQRM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 34478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34478
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_677_length_34478_cov_14.105337"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PKK81_01965"
     CDS             complement(<1..926)
                     /locus_tag="PKK81_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31662.1"
                     /translation="MSRIQPRLRILSLAIAMLACLPSLLLAQTLPQQLRVIDTPSDGG
                     GSLTVLWAPSASDSAAWKYQILLHEGSVPSDPSAWKVVAEFPANTRYVREGKTAWWTR
                     SGEPGDHIFLLKNGKGIELKANQSYAVTVAAANGQERVIALPIVASPEPNWMNWNQLN
                     NLVLALMFGGVVFYAINLAKRKEIFLRRIPGLDAVDEAIGRATELGKPILYMTGAHDM
                     NDPSTIAAAVILGRVAKKAAAYETELLVPHREPITMAVCQEITKQAYMEAGKPDLFKD
                     DANFFITSDQFSYTAAVDGIMLRRKPAANFFMG"
     gene            complement(923..2050)
                     /locus_tag="PKK81_01970"
     CDS             complement(923..2050)
                     /locus_tag="PKK81_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31663.1"
                     /translation="MAHSYTPGLTVTEQTTVRRRRILPLPGTVLVKVGDVVRADTAVA
                     RAELPGKVVPLNLANQLGIAPDEINDYLIKKEKDSVQKDDVLAENKPFIKWFKTEIRS
                     PITGKVESVSTITGQILLREPPRILELRGYIDGSIVEVHPEQGVTVETSCSLVQGIFG
                     IGGETSGELVMGVTAPDQPLTPELLTPAMKGKIVIGGAFLSAATMTRAKELGVIGLVV
                     GGIHDKDLRALLGYDLGVAITGTEQVGFTLILTEGFGTIPMAPKTFALLSAHAGHKAS
                     VSGATQIRAGVIRPEIIIPQTSRTGAARPDRVEREGIQLGDPVRVIRDPLFGKIGAVS
                     GLPSELRAIPTESEVRVLEVRFPDGTTTVVPRANIEVIEGA"
     gene            complement(2365..4215)
                     /locus_tag="PKK81_01975"
     CDS             complement(2365..4215)
                     /locus_tag="PKK81_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNV31664.1"
                     /translation="MTAQPHSPAAPAVTHPLNVIVATDCGSTTTKAILIEKVGEEYRQ
                     TYRGEAPTTVEAPFEDVTRGVLNAIAEIEELSGRTILDGDRIITPNQAAQGDPKRGVD
                     IYVSTSSAGGGLQMMVTGVVQNMTGESAQRAALGAGAIVIDVLASNDGRLPYEKIERI
                     RTMRPDMILMSGGTDGGAVTHVVEMAEYIAAADPRPRFGSTYRLPLVYAGNTDARPQI
                     KTILGEKTALDFADNIRPVLERENLAPARNKIHDLFLEHVMQQAPGYKKLIEMAGAPI
                     MPTPAAVGVIMETIAKREGINLIGVDIGGATTDVFSVFNGLFNRTVSANLGMSYSISN
                     VLAEAGLDNIMRWVPFTIDEQTLRNRIKNKMVRPTTIPQSLDELQIEHAIAREALRLA
                     LIHHKSLATGLKGIQQERTISDVFEQRTSGKTLIDLLKLDLIVGSGGILSHAPRRIQS
                     MLMMVDAYEPLGVTTLSVDSIFMMPHLGVLSTVNEQAATDVFVRDCMVYLGTCIAPIG
                     QGKDGERCADYEIALPDGRIEKGQLAFGDLKLFALAREQQATVTMQPAKQIDLGNGPG
                     QSFTKTVKGGVVGLMLDGRGRPLQLPGEQAARVAMLTRWYQAVGLYPVGS"
     gene            complement(4212..4586)
                     /locus_tag="PKK81_01980"
     CDS             complement(4212..4586)
                     /locus_tag="PKK81_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31665.1"
                     /translation="MSAFSAGFLSRRAQDFRAGLRHAFAVRPEQAAFTIEDLALLERV
                     ADAVVSRGMAAPATLFLESMGPMNFLGSQALHFLAPVVECVLTGTEIAQVARLLERRD
                     SIHRLTALIEAKAAAPQGAPAR"
     gene            complement(4685..5131)
                     /locus_tag="PKK81_01985"
     CDS             complement(4685..5131)
                     /locus_tag="PKK81_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31666.1"
                     /translation="MRWVHVLLLTIIVLLPAGKATAKVYRCQGASGSTVLTDQPKGKT
                     GCVAIDTATPPLPGGFTPPVEPSVPAQVELPANAIPPSISAAPMVPRQPGPGDPALSP
                     AVAKDAPEKAPPEAQRCSPRVNPLNPFAGMNCSPASGEPPAENKNQ"
     gene            complement(5269..5622)
                     /locus_tag="PKK81_01990"
     CDS             complement(5269..5622)
                     /locus_tag="PKK81_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31667.1"
                     /translation="MSTPAGEKHQLAGFDARERGFNRPVEFVRTASGYQATLRYETTH
                     IVTPEAPSAGEALQQLITTLQQEGYTQLRSQQSYRDGLYLGSQELWVEYPDRQAAPQV
                     GLLGLLKKWLGRSPG"
     gene            complement(5630..5998)
                     /locus_tag="PKK81_01995"
     CDS             complement(5630..5998)
                     /locus_tag="PKK81_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31668.1"
                     /translation="MADNTLTPKHIEIAETFVRLYVFLTQYIDRCEDEAARKEYPEAE
                     LQKHLGETRAKMMEILKVNPVVKGKVEKECERVLALGSKCLMGGTEKVAALDVLGAER
                     VILKNKTIALSDLLAVYRAL"
     gene            complement(6077..6382)
                     /locus_tag="PKK81_02000"
     CDS             complement(6077..6382)
                     /locus_tag="PKK81_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNV31669.1"
                     /translation="MGELIRIAGTADVKPGAGMVAEVNGKAIAVFNVDGAFYAIDNTC
                     VHRGGPLGEGDVEGDVVSCPWHNWQFNVKTGTCVNNPSAKVTAYEVTVEGTDIKVRL"
     gene            complement(6427..7314)
                     /locus_tag="PKK81_02005"
     CDS             complement(6427..7314)
                     /locus_tag="PKK81_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNV31670.1"
                     /translation="MAEQQGGAPHQEEAAAKPNIIPGVKHVVAISSGKGGVGKSTVSV
                     NLAVALALTGAKVGLLDADIYGPNIPMMMGVEKTPEQKDGKIAPAESHGVKLISMGFF
                     VPEDTAVVWRGPMVHTAIQQLFRDVLWGDLDYLLIDLPPGTGDAQLTLTQLVSLSGAV
                     TVTTPQEVALHDVRKGMMMFQKVNVPLLGIVENMSFFLCGHCGERTEIFSHGGGERAA
                     EKLGIPFLGRVPIDPAIRAGGDTGNPIVVAKPDSPQAQAFREIAAKLAAALQTGEAGA
                     AKSRIASLLDKIKRPATGN"
     gene            complement(7574..8407)
                     /locus_tag="PKK81_02010"
     CDS             complement(7574..8407)
                     /locus_tag="PKK81_02010"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNV31671.1"
                     /translation="MADSTTQSVCWGTVALFTAITLLTFVGVPLFAYFYDYTLLDWVL
                     LAVLYIVTGMGITVGYHRMITHRSFESHPWVKACLLVMGGWAVQNSALMWCADHIRHH
                     AHTDEEADPYNASKGFWHSHVGWLFFDTPYREDRFAAKLRTDPMIMWQHRYYWVIVAS
                     GFLLPFALGYLNGGFTSGLGCFLLAGVLRTVLVLNSTFTINSLCHMVGTQPHGTQDSS
                     RDSWLISFVSFGEGYHNYHHTYSHDYRNGSKWYNFDPSKWLIYSLSLFGLTYNLHRER
                     T"
     gene            8794..11775
                     /locus_tag="PKK81_02015"
     CDS             8794..11775
                     /locus_tag="PKK81_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNV31672.1"
                     /translation="MPLDPAPLEPRIRAIGEDLARRSGGRAPGLFDSRWWSQAAINLA
                     MKDPAFKAQLFRFIDVLPSLHDDARVVQLAEEYFGEMSAHLVGAQWGLKALASTKLGA
                     SLSGKAIRHQVEQMATSFIAGASIEQAVPTLRKLWEDGRAFSVDLLGEASVSEREADA
                     YRDRCLAALQALGEACPGWSPAPLLERDHLGPLPRVQLSIKISALYSQLDPIDPESSY
                     RAVAARLRPLLNLAGTLPASVIFDMEQADTKELILSIFMRLFMEEPYRTFPHAGIALQ
                     AYRTDTYDDVQRLLTWSRERLVPVTIRLVKGAYWDSDAIRYRQRGWPMPLFERKTQTD
                     VNYERLVRLLLEQSHVIRPAFGTHNLRSLAVVEAVAEALKLQSQAWEYQMIYGMAEPF
                     QHAMSDRGRRLRLYAPVGELLPGMAYLVRRLLENTSNESFLRKEYVESQPLSLLLSPP
                     DATLPSPPSPMAPEAGASSTVGSAHFVNEPAADFSRDDVRVAMAEAIDQVRKQLGQRL
                     EVSRLNTHLPTGPDLHSFNPGQPEQLVGIVQSYLPADVAGLLKVAESHAEEWKHRAVA
                     ERVAVMRKAASLMREQRWELAAWEVFEEAKPWREADADVAEAVDFLEYYAGEMEQLDP
                     PPRLGRYPGELNELFWSPRGVTVVIAPWNFPLAIPAGMVAAALVSGNPVLFKPSERAS
                     IMGYRLATILRQAGVPAGVLQYVPGGPDVGRELVTQASVATIAFTGSKDVGLGMVQAA
                     AVQQPGQKFVKRVIAEMGGKNAIIVDDTADLDEAVTGVVQSCTGYAGQKCSACSRVIL
                     LDSIHDAFIQRLADAVMSLRIGAAEDPATQVGPVIDGRAQKRIRDYLEIGRREGRVVV
                     DRSMEGPGHAVGPVVIAGIEPTHRLAQEEIFGPVLAVMRASSFEQALQLANGTAYALT
                     GGLYSRSPRHIELARQAFDVGNLYINRSITGALVGRQPFGGHRLSGIGAKAGGEEYLK
                     QFMVARVVSEQTLRRGFAPTL"
     gene            complement(11665..12570)
                     /locus_tag="PKK81_02020"
     CDS             complement(11665..12570)
                     /locus_tag="PKK81_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31673.1"
                     /translation="MIVQTLVRSTVMAVGMCGFLGIPSAPGQARATEAGQTIAQSADY
                     FPDTVGTRWQYRGQISEGPLQTIENKYFFNVSSVTGTRTLKGGVVVNVFHDTNPGNHG
                     ASDSFYRRDAAGVVYYGSEPGTPLEKQLVPYQIIRFPMKVAQSFQQFNRMDIDFGSDI
                     DGDGTNEHVDVEGVSTVIGQESITVPAGTYPDAVRVEARMTLKIHLSSADRTAVGTDV
                     MTAWFAKGVGLVKYIERQELAALKEDRGNTTDITEELEEVTVKSAPALQGRSESSPER
                     LLADDTRDHELFQVLFAPGLRPYTR"
     gene            12680..13435
                     /locus_tag="PKK81_02025"
     CDS             12680..13435
                     /locus_tag="PKK81_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV31674.1"
                     /translation="MSAFPPAAQDVRERRTVLVTGGSGGIGRALCLAFAHAGYWVGVH
                     YFRQRPAAEQTLALLSQAGGQGALYQADVRSSHEVQAMLDAIRAQRGRLDTLLCNAGI
                     ASKHLVVNCPEEAWQQIIDTNLTGTYRCMTAAVNDMMPQGGGSIVVIGSFAGLQGTNG
                     QAAYAASKAGLIGLVKTAAREWGPLNIRVNMVCPGWQQTRMAGDSFPTGDRLDDHVLG
                     RVSNLADVSRTICQLAQLSDMSGQVWNLDSRIL"
     assembly_gap    13785..13884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13885..14628
                     /gene="bioD"
                     /locus_tag="PKK81_02030"
     CDS             <13885..14628
                     /gene="bioD"
                     /locus_tag="PKK81_02030"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNV31675.1"
                     /translation="TGTDTGVGKTLVSSALVRRLVQTGRSVGVMKPVETGVLPEQLSE
                     SDAGRLMAAAGVSDGIDEVSPYRFPAPLAPFAAASAQGSVIEMQEIMRRYERLAARDS
                     CVVVEGAGGLLVPMGKDWDMRDLIGRIGLPVVLVGRAGLGGINHALLTLEGLRQRHCA
                     VMAVVLNETALSTTPAQQEQCASTVALLRERAGVPVLGPLPYQPQLGTDWSGAVEALA
                     QSSPIRELANLVWANAVGMSGSPRRGPEP"
     gene            complement(14552..>15169)
                     /gene="thiE"
                     /locus_tag="PKK81_02035"
     CDS             complement(14552..>15169)
                     /gene="thiE"
                     /locus_tag="PKK81_02035"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNV31676.1"
                     /translation="LVLDPTVRPDRPLPDLLREAAAHGVRLFQYRDKQATMRDAYAKA
                     LALRQAATDAGALLIVNDRCDLAMAVNADGVHLGQDDLPISYARRLMGPGKIIGLSTH
                     NAAQVREAVTAQPDYVGFGPIFSTSTKADHDPVVGLEGLRAARALTTLPMFAIGGITA
                     ESVGEIMAAGADGVAVISAILKAPDLGAAIRTFQQHLPTPDSQAP"
     assembly_gap    15170..15269
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15290..16639)
                     /gene="accC"
                     /locus_tag="PKK81_02040"
     CDS             complement(15290..16639)
                     /gene="accC"
                     /locus_tag="PKK81_02040"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNV31677.1"
                     /translation="MFKKVLVANRGEIALRVIRACKELGIKTVAIHSEADAASLHVRA
                     ADEHVCVGPPEAALSYRNIPNVLSAAEVTGADAIHPGYGFLSENAHFAEVCESIGVKF
                     IGPTSENIALMGDKSKAREVVAKKGLPVTPGSPGELRSEQDAQEAAKTIGFPVIIKAS
                     AGGGGRGMRVVNKPEELARAFQAAQAEAKSTFGHDGVYLERYFLEPRHIEVQIVADNR
                     GHVVHLGERDCSIQRRHQKLVEETPSPAIDEGLRREIGRVAVQAVKAIRYCNVGTVEF
                     LLDKDRNFFFMEVNTRIQVEHPITEMVTGIDLVKEQIRIASGMPLSFKQPDIKLNGHS
                     FECRINAEDPEKFTPCPGQITKYSAPGGFGVRVDSAMEPNAVVVPYYDSLIAKLITHG
                     RDRQEAMARMRRALDEFVIEGIKTTIPLHRKIFNDPDFQKGHVSTTFLDRFLAGQSS"
     gene            complement(16821..17396)
                     /gene="accB"
                     /locus_tag="PKK81_02045"
     CDS             complement(16821..17396)
                     /gene="accB"
                     /locus_tag="PKK81_02045"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNV31678.1"
                     /translation="MSSSGKKTGKGRGAGKPIVLPSAFAPRSTAAETLLSPDQAAQIQ
                     QLADLLKRNHLTELEIERSGVRIRIRHEPVIRTTTAQTVETATNQSTSTGATPAAQTR
                     PPAETDGQVTITSPIVGTFYRSPSPDADPYVEEGDYVRKGQVLCIVEAMKLMNEIESE
                     ADGRITKILAESTKPVEYGQPLFLIDPNATP"
     gene            complement(17393..17953)
                     /gene="efp"
                     /locus_tag="PKK81_02050"
     CDS             complement(17393..17953)
                     /gene="efp"
                     /locus_tag="PKK81_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNV31679.1"
                     /translation="MISTAEFRNGSPLMVEGQPFYIVEFQHVKPGKGGAFVRTKLKSY
                     LSGNVLDRTFRSGEKFDTPQIEECDMQFLYATNDSYTFMDTETYEQSTYEKSQLGSNA
                     DLLKENMIAKILIYEHRPITVLLPNFIELKVVDGEPGVRGDTASGGNKPVIVETGATI
                     KVPLYLEIGETIRIDTRTREFVERVR"
     gene            complement(17979..18476)
                     /gene="aroQ"
                     /locus_tag="PKK81_02055"
     CDS             complement(17979..18476)
                     /gene="aroQ"
                     /locus_tag="PKK81_02055"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNV31680.1"
                     /translation="MLRLLVLHGPNLNLLGTREPSVYGQASLPDIDKSIVRRAGERGI
                     AVQTKQTNVEGELVTWIQNARGHFDGIIINPAAYTHTSIAIRDAIAAVALPTVEVHLS
                     NIHQREEFRHHSFIAGVALGQISGFGPTGYLLALDALAAHLEAVSKASRPRTAPKNKP
                     TASRR"
     gene            complement(18617..19294)
                     /locus_tag="PKK81_02060"
     CDS             complement(18617..19294)
                     /locus_tag="PKK81_02060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV31681.1"
                     /translation="MASSSRIDPSTAAEIDRLATAVAKDPRSKEFLPLADEYIKVGMW
                     QEAAGVLEDGLKVYPGFVTAMAALGRVYDQLGQAVKAKAILEDVVRQRPDNLRAHRIL
                     AKLYHAEGNADLALLSCTAILNANPFDEEAASVKRSITGAPDNPPAAKREKKRIVTEP
                     RPDGTKAGAHITPAPEPTVSTSSMASEPVTETPGRPTPDLPVVKHAAAIARLEAWLRT
                     IQAQRVH"
     gene            complement(19298..20557)
                     /gene="coaBC"
                     /locus_tag="PKK81_02065"
     CDS             complement(19298..20557)
                     /gene="coaBC"
                     /locus_tag="PKK81_02065"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNV31682.1"
                     /translation="MQTTGPSLTGVHLVLAVTGSIAAYKAVGLLRLLRREGATVNVVM
                     TAGACRFVTPLTFEVLSGSHVATDLFEAHQEMLHLSLPEQAKAILIAPATANCLAKAA
                     LGLADDLLSTMLLTTQCPVIFAPAMDGDMWQHPTVVEHVATLRTRGAVIVEPEEGPLA
                     SGRSGQGRLADEARILSVLQATLHPRRDWSGRRLLISAGPTQEAIDPVRFISNRSSGK
                     MGYALAEAAQARGAEVLLVSGPTALTPPAGVELCPVTTAEEMRKAVISRFSWSDTVIM
                     AAAVADFRPTQPAAQKLKKRRSPVTSLELSPTDDILRELGERRTTQVLVGFAAETEDL
                     LAHAREKLQAKDVDLLVANDVTAAGSGFGSDTNRVFILDRDGRAEELPLLPKREVADR
                     ILDRVLTLPTGRRSTKPAAGTGTHRKE"
     gene            complement(20567..20929)
                     /gene="rpoZ"
                     /locus_tag="PKK81_02070"
     CDS             complement(20567..20929)
                     /gene="rpoZ"
                     /locus_tag="PKK81_02070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNV31683.1"
                     /translation="MGEMLSLLPEYATGEFDSRHRLVIIAAQRAKQLVQGARMSGPSK
                     FTKETSIALDEVLRHKVKFLIGQEAREAIKESKRVKEGETERLAMMTGEDAKEIKKEL
                     SVYVDDTVKPAPAPEGEE"
     gene            complement(20922..21638)
                     /gene="gmk"
                     /locus_tag="PKK81_02075"
     CDS             complement(20922..21638)
                     /gene="gmk"
                     /locus_tag="PKK81_02075"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNV31684.1"
                     /translation="MNTAPVSSNDKPPQVRRGILFIISAPSGSGKTTLCKQITANVPG
                     LWHSISYTTRKPRPGEVDGQDYHFLDEPAFRQMIDRNEFVEWAHVYGNLYGTPRKMLT
                     EKMEQGIDVLLEIDVQGARSVKKKFEDGVYIFILPPSFDTLRTRLQNRAGDSPDEIQR
                     RLQKAKEEVWSYREYYYIVRNDDLKQSLKELESIFLAERIKTKRLNMTWLEEKFILDK
                     EGKQGNSSSAPASKEHLHHG"
     gene            complement(21669..22547)
                     /locus_tag="PKK81_02080"
     CDS             complement(21669..22547)
                     /locus_tag="PKK81_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNV31685.1"
                     /translation="MIRSMTGYGKKDGTSQKAGVTVEIRSVNHRFLEVAVRLPRSLAQ
                     LEDQVRKTVQQRCLRGRVDVSVSVHSAGGSLKTVQIDQALAKQYHTALKKLQKTLGLR
                     GAVDVSTLAGFRDILSISDEPVDAGQLTKTVLRVLGGALTDLEKMRRREGEALAKDLA
                     LHLDAIREAKSLVAEKAPVLAKHAFERMKGRIETLLQAELPDPARLQQELAVFADRSD
                     ISEELVRLESHMLQFDQQLHSKESVGKTLEFLLQEMGREVNTIGSKANDADIAALVVR
                     MKAELEKLREQVQNVE"
     gene            complement(22544..23215)
                     /gene="nth"
                     /locus_tag="PKK81_02085"
     CDS             complement(22544..23215)
                     /gene="nth"
                     /locus_tag="PKK81_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNV31686.1"
                     /translation="MKVATTDQPAGVTTKRRLARMLTALRATSPAMKVELDHRTPWEL
                     LVATILSAQCTDQRVNQVTPNLFRRYQQPRDYASADPTELEALIRPTGFFKTKARNLI
                     HCAKAVAGQFQGEVPDTMEALTTLPGVGRKTANVLLGNAFEKPAIVVDTHVKRVAGRL
                     DLTRHTDPEKIELDLQRLLPADQWTEGSQRLLLHGRYICLARTPKCQHCPIYADCHWK
                     GKTAR"
     gene            complement(23249..25018)
                     /gene="hflX"
                     /locus_tag="PKK81_02090"
     CDS             complement(23249..25018)
                     /gene="hflX"
                     /locus_tag="PKK81_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNV31687.1"
                     /translation="MRYRHRRAAKRCVADPNVRVPRSITIPAIHGHVTGLKASHLAAL
                     ERLYRRRVPSSAVLTTELARTTSQLTREIRRPIGLLITRRGVIEQVLVGTGCAPTFES
                     LAKFRVGSHSLRGLRLIRTHLHDDPLSQDDLTHLALLRLDLIGILGVDEKGDPSLLHL
                     AHLLPPNQQGEVCRILKPVPFHDLDLQLDTFLHALESDLQRSDTKHDVAAGTESAILV
                     SAAPKSHAEQEEHLEELNELAESAGVRVLDRIAQRTHEGYERYLLGKGKLKEVVMRAL
                     QKGADLVIFDQDLAPAQARAISEVTDLKVIDRTQLILDIFAGRAHTREGKVQVELAQL
                     RYLLPRLSGHGTSLSRLGGGIGSRGPGETKLETDRRRIRDRIAHLEREIDDVARHQDQ
                     RRSRRVRQGLPILSIVGYTNAGKSTLLNTLTHSQIPAQDRLFETLDTTSRRLRFPHDR
                     EVIVTDTVGFIRDLPKDLVGAFRTTLDELRDADILLHVVDASAPNVDQQITAVETVLQ
                     SLNLDTIPRVMVLNKCDRLSAHEAGVLCQRYHAIGISAPNRETLRPLIAHLESLLPAI
                     PTPPGYEEDPDQPPQDLALASHS"
     gene            complement(24969..26027)
                     /gene="tsaD"
                     /locus_tag="PKK81_02095"
     CDS             complement(24969..26027)
                     /gene="tsaD"
                     /locus_tag="PKK81_02095"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNV31688.1"
                     /translation="MKPPASTTILGIETSCDETAAAVLDGEGHVLANVISSQQTVHER
                     FGGVVPELASRAHIQNVDHVTRQALDAAGLTWQDLGAIAVTQGPGLAGALLVGISYAK
                     SLAYALGIPLVGVSHLEGHIASAWIDRPSFPRTCVVLIASGGHTHLFHATEHGGFHLM
                     GRTIDDAAGEAFDKGAQMLGLGYPGGPLIDAAARKGSPAAVRFPRSRVRTGQLDFSFS
                     GLKTALLYHLQRMDQEAIVRQQADLAAGYQEAIVDVLVRQTFAAVRQAGVSALAVVGG
                     VSANSRLRARLTERAAGEGIELGLPALSYCTDNAAMIAAAGQRALEQGRLASFECEAM
                     PNYALPASSGGETMRRRS"
     gene            complement(26276..28705)
                     /locus_tag="PKK81_02100"
     CDS             complement(26276..28705)
                     /locus_tag="PKK81_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNV31689.1"
                     /translation="MFERFTDKGRKIIILAREEAERHQNDYLGTEHLVLAILRETDGI
                     ALMILKKMGLSTEQIRLEIERNLPGGGTTMTFGEIPFSPRVKKVIEYGVEEARLLGHN
                     HIGSEHLLLGLLREEEGIGGKILRSLGANLLTARQLTVTFLRKSAPRERDRKSNTPAL
                     DEFGRDLTQLAQEGQLDPVIGRADEIERVLQILSRRSKNNPVLIGESGVGKTAIVEGL
                     AQRIIASEVPDNLLSRRVIALDLGSLVAGTKYRGQFEERLKVVMKEIVQAGNIIIFID
                     ELHTLVGAGAAEGSIDASNMLKPALSRGEIQCIGATTLDEYRKHIEKDGALKRRFQPI
                     HVQPPSTDETVRIIQGLRDRYEEHHGVEITEDAIVEAVKLADRYITDRFLPDKAIDLI
                     DETGSRAKLQTYALPTELKALEQELKKISRDKELAISMQNFEEAVRHREEEERLRKLL
                     DESKREWKKNQEKHKPTIGKEEVAYVVSKMTGIPLFKLEEEESNKLLRMEEFLHKRVI
                     GQNEAISAVARAIRRSRAGLKEAKKPIGSFIFLGPTGVGKTELARTLAEFLFNSEDAL
                     IRIDMSEYQEKFTSSRLFGAPPGYVGYEEGGQLTEKVRRRPYSVVLFDEIEKAHPDVF
                     NVLLQVLDDGVLTDSLGRKVDFKNTVVIMTSNIGTKMIQKGVSLGFQNDEKGGQALRR
                     KEDVMGELKRSFSPEFLNRIDEIVIFHSLEKEHLIHILDILLHELNLRLIDKSVSIEV
                     DDEVKQWLIKEGYEPLYGARPMRRIIQRAIGDPLSEELIKGRFKENRKIKVVLRDGAP
                     AFIEQEAMAGV"
     gene            28992..29852
                     /locus_tag="PKK81_02105"
     CDS             28992..29852
                     /locus_tag="PKK81_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNV31690.1"
                     /translation="MKSKSIGILTKPKFPEVKSTVQAVVSWLRSRNIDVLLDTTATTL
                     LGEQGGFQKTHLASKADVLLVLGGDGTMLNAARLAGERGIPILGVNMGGLGFLTEVVL
                     DNLYPSLERMFANDFVLDERLMLKTHVHRHGETVARGVVLNDVVISKGTLARMIELKI
                     AIQGQFVTNLRGDGLIISTPTGSTAYSLSAGGPIINPAVPSLILTPICPHTLTHRPLI
                     VPASAEIEVVLTSKDDGAMATLDGQVGVALTQGDTVEIRASEHMTRLIRFPESSYYEV
                     LREKLKWGDG"
     gene            complement(29874..31244)
                     /locus_tag="PKK81_02110"
     CDS             complement(29874..31244)
                     /locus_tag="PKK81_02110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV31691.1"
                     /translation="MSATGSETSGHVVIVVGAGPAGMALAKKMADAGHEVIILNRDIK
                     FGGLAEYGIFPSKLKLRGGLKKQYWDMLEQPNVHYFGNVSVGQGKDLTVEDLRGLGAS
                     AVAFSIGAQGTKAIGVEGDSAKGVFHAKDVVYHFNRLPGFGDRPFDMGKHVAIIGVGD
                     VMVDIAHWLIKYKKVERVTAIARRGPVERKYNPKEIRAVCSNMDQEGIATEFARIKDR
                     LAAVGQNADEVLASMTAEFTKCEPTGSPSKMGFRFLASPKRVLVDANNRVRALEMEEN
                     KLEPKGEDTAAVGLKQLYEFPVDSVVFAVGDRVDETVGLPYKNGVYVTNPNKTGNDPD
                     DSLFQAYDEKSGQVVDGVFLAGWARKASEGLVGIAKRDGDWCAEVISRYLTTQATRSR
                     GTMDGVIAKLHTILKDRKSRPVDLEGLKVLDTVEKAHAASPEAIGEFKFASNADMLAH
                     IERGRG"
     gene            complement(31427..32881)
                     /locus_tag="PKK81_02115"
     CDS             complement(31427..32881)
                     /locus_tag="PKK81_02115"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNV31692.1"
                     /translation="MKKASRTTGTAASMTTSTRMERDTMGELPVPSNAYYGVQTARAI
                     ENFPISSLRIPRAMIRAMGLIKRAAASVNHSLKLLDKKPADAIVRAATEVVEGRLDAE
                     FPVDIFQTGSGTSTNMNTNEVISNRATELLGGARGSKLVHPNDHVNLGQSSNDVVPTA
                     IHIAASETIQRQLIPALTQLHKALNGKAREFDKIVKIGRTHLQDATPVRLGQEFGGYA
                     RQIELGIARMKRAQAALSEVALGGTAVGTGLNCHPKFAGKVMAIISKETGCAFKEAVN
                     HFEGQSAQDSLVEASGDLRTLAVSLMKIANDIRWLGSGPRCGLGEINLPETQPGSSIM
                     PGKVNPVIAESVTMVCAQVIGNDVTITVGGQAANFELIVMLPVMAYNLLQSIELLATA
                     STNFAVKCIDGIKANEERCKSLIEESLAMCTALAPVIGYEAAAKLAKDAYKSGKTVRE
                     VAREQNVLPEKRLTQLLDPWRMTQAGGPVGSAGG"
     assembly_gap    33087..33186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(33245..33700)
                     /locus_tag="PKK81_02120"
     CDS             complement(33245..33700)
                     /locus_tag="PKK81_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNV31693.1"
                     /translation="MAIVRWDPFRELEEMSDRLNRMIARPSTGTATGQGKEVMTVADW
                     TPTVDISETESEYAIKAELPEVKKEDVKVTVEDAVLTIQGERKQEKEEKGKKYHRIER
                     SYGRFVRSFTLPDSVDESKVRAEYADGVLHLHLPKSEKAKPKQIDVKIS"
     gene            complement(33990..>34478)
                     /locus_tag="PKK81_02125"
     CDS             complement(33990..>34478)
                     /locus_tag="PKK81_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNV31694.1"
                     /translation="LKGPLHGGANEEVVMMLREIGTPAQARAAVEARLEGKHKLMGFG
                     HRVYKVKDPRATVLQDLCMRLFNVCGTSPLYEVAVAVEQLAGERLTDKGIYPNVDFYS
                     GIIYDKMGIEVDLFTPLFAMARVSGWLAHWLEQLRENKLYRPDQIYSGEHDRHYVPID
                     KR"
BASE COUNT         6172 a        10004 c        10556 g         7446 t
ORIGIN      
        1 cccatgaaaa agttggccgc cggttttcgg cgcagcatga tgccgtccac cgccgccgtg
       61 taactgaact ggtcgctcgt aatgaaaaag ttcgcgtcgt ccttgaagag gtccggtttt
      121 ccggcttcca tatacgcttg tttggtaatt tcctggcaga cggccatggt gatcggctcg
      181 cggtgcggga ccagcaactc ggtctcatac gcggcggcct tcttggccac gcgccccagg
      241 atgacggcgg cggcaatggt cgaagggtcg ttcatatcgt gcgccccggt catgtagagg
      301 atcggcttgc ccaattccgt ggcgcgcccg atggcttcat cgacggcatc cagcccggga
      361 atacgccgca agaagatctc tttccgcttg gccaggttga tggcgtaaaa cacgaccccg
      421 ccgaacatca aggccagcac caggttgttc agttggttcc agttcatcca gttcggttca
      481 ggcgaggcga cgatcggcag tgcgatcacc cgttcctgtc cattcgcggc ggccaccgtg
      541 acggcatagg actggttcgc tttgagctcg atgcctttcc cgttcttgag caggaagata
      601 tggtcgccag gttcgcctga tctcgtccac caggcggtct taccctcgcg aacataccgg
      661 gtgttggccg gaaattccgc gacgactttc catgcggacg ggtctgaagg cacgctgcct
      721 tcatgcaaca ggatttgata cttccacgcg gcgctgtcgc ttgccgaagg agcccagagg
      781 accgtgaggc tgcctccccc atcgctgggt gtgtcgatga ctcgaagctg ctgcgggagg
      841 gtctgcgcca agaggagcga cggcaggcac gcgagcatcg cgatggcgag ggacagaatg
      901 cgaaggcgag gctgtatgcg gctcatgctc cctctatcac ttcgatattg gcgcgaggca
      961 ccacggtggt cgtgccgtcg ggaaaccgga cttcgagcac gcggacttca ctctccgtcg
     1021 ggatcgctct gagctccgag ggcaacccgg acaccgcccc gatcttgccg aacaacggat
     1081 cgcggatcac tcgcacggga tcgccgagtt gaatcccttc gcgttcgacc cggtccggcc
     1141 gggcggctcc ggtgcgtgaa gtctgtggaa tgatgatttc cggacgaatc acgccggcgc
     1201 gaatttgcgt cgcacccgaa acagaggctt tgtgccctgc gtgggcggac agcaacgcga
     1261 acgtcttggg cgccatcggg atcgtgccga agccttccgt caggatcagg gtgaagccca
     1321 cctgctcggt gccggtaatt gccacgccca gatcataccc gaggagcgcg cgcagatctt
     1381 tgtcgtgaat cccgcccacg acgagaccga tgacgccgag ttccttcgcg cgagtcatgg
     1441 tggcggcgga gaggaatgcg cctccgatca cgatcttgcc cttcatggcc ggcgtcaaca
     1501 gctcaggcgt cagcggttga tccggcgccg tgacacccat gaccaattca ccgctggtct
     1561 ccccgccgat gccgaagatg ccctgcacca gactgcagga ggtttcgacc gtcactccct
     1621 gttcgggatg cacttcgacg atcgagccgt cgatatagcc gcgcaattcg aggatgcgcg
     1681 gcggttcccg caacagaatc tgtccggtga tggtggagac ggactccacc ttgccggtga
     1741 tgggcgagcg aatttcggtc ttaaaccatt tgatgaaggg tttgttctcg gccagcacgt
     1801 cgtctttctg gaccgagtct ttttccttct tgatcaaata gtcgttgatc tcatccggcg
     1861 caatacccag ctggttggcc aaattgagcg gaacgacctt gcccggcaac tcggcacggg
     1921 cgaccgcagt atcggcgcgc acgacgtcac ccaccttgac caggacggtc cccggcagcg
     1981 gcagaatacg cctgcggcga acggtggtct gttcggtgac ggtcaggcct ggtgtgtaac
     2041 tgtgcgccat tgtcggttgc tgaaaacgcc ctaccgcttc gttctcagtt gctcgctccc
     2101 ctcaccgtac gacttccggt acgactcggg gctctcgcgc cttgcggcct cgctgcaggg
     2161 cgttttgagc aacctcctca agataagagc tgatggctaa tagccgatag gagctagtct
     2221 tcatacaaga gcgaactgca aatgctttct gtgaacagtt tgcatccagc atgcggtgcg
     2281 aggcttctct tctccttgcc atctgttata tgctccccgc tcttcaccat acgctctacg
     2341 ccatccgctc ccagcattcg actctcagct ccccaccggg tatagcccta ctgcctggta
     2401 ccatctggtc agcatggcga cgcgggctgc ctgctcgccc ggtaattgta acgggcgacc
     2461 acggccgtcg agcatgaggc cgaccacacc acccttcacc gtttttgtga acgactgtcc
     2521 cggtccgttc ccgagatcga tctgtttggc cggttgcatc gtcaccgtgg cctgctgctc
     2581 ccgcgccaag gcgaagagtt tgagatctcc gaacgccagc tggcccttct cgattcgtcc
     2641 atcgggtaac gcgatttcgt agtcggcgca gcgctccccg tctttcccct gtccgatcgg
     2701 ggcgatgcag gtgccgagat agaccatgca atcacggaca aagacatcgg tggcggcctg
     2761 ctcgttcacg gtggagagga cgccgagatg cggcatcatg aagatgctgt cgaccgagag
     2821 cgtcgtgaca ccgagcggtt cataggcatc gaccatcatc agcatggact gaatacggcg
     2881 cggcgcatgg gacaggatgc cgccgctgcc gacgatcaag tccagtttca acagatcgat
     2941 cagggttttg ccggaagtcc gctgctcgaa cacatcggaa atggtgcgct cctgctgaat
     3001 gcccttgagt cccgtcgcaa gcgatttgtg gtggatcagc gccagccgca gggcctcgcg
     3061 cgcgatggca tgttcgatct gcagttcgtc gagcgattgc ggaatcgtcg tgggccgcac
     3121 catcttgttc ttgatgcggt tgcgcagggt ctgttcgtcg atggtaaacg gcacccagcg
     3181 catgatgttg tcgagccccg cctccgccag cacattcgaa atgctgtagg acatgccgag
     3241 gttggcgctg accgtgcggt tgaagagtcc gttgaacacc gagaagacgt cggtggtggc
     3301 gccgccgata tcgacgccga tcaggttgat gccttcgcgt ttggcgatgg tctccatgat
     3361 gacgccgacc gccgccgggg tcggcatgat gggcgcgccc gccatctcga tgagtttctt
     3421 gtagccggga gcctgctgca tgacatgttc gagaaacagg tcgtggattt tgttgcgggc
     3481 cggcgcaagg ttttcgcgct caaggaccgg acggatattg tcggcaaaat ccaaggcggt
     3541 cttttcgcct aaaatggttt tgatttgcgg ccgggcgtct gtgttgcctg cgtacacgag
     3601 cggcagccgg taggtgctgc cgaagcgcgg acgcggatcg gcggcggcga tatactccgc
     3661 catttccacc acatgggtca cggcgccgcc gtcggtgccg ccggacatca ggatcatgtc
     3721 cgggcgcatg gttcgaatcc gctcgatctt ttcgtagggc agccgtccgt cgttcgaggc
     3781 caagacgtcg atgacgatgg cgccggcgcc cagagcggcg cgttgagcgc tctcaccggt
     3841 catgttctgc accacgccgg tgaccatcat ctgcaatcca ccgccggcgc tgctggtgga
     3901 cacgtaaatg tccacgcctc gcttgggatc gccctgagcc gcctggttgg gtgtgatgat
     3961 gcggtcgccg tccagaatcg tgcgcccgga gagttcttcg atttccgcga tggcgtttaa
     4021 cacgccgcgg gtcacgtctt cgaacggggc ttcgacggtc gtcggcgctt caccacggta
     4081 ggtctggcga tactcctccc cgaccttttc gatcaaaatg gctttggtgg tggtgctgcc
     4141 gcaatcggtt gcgacgatga cattcaaggg atgagtcact gctggtgccg ccggcgagtg
     4201 aggctgtgcc gtcatcgagc gggcgctcct tggggagccg cggccttggc ttcgatgagg
     4261 gcggtcaggc ggtgaatgga atcgcgccgt tccagcaggc gcgcgacctg cgcgatctcc
     4321 gtaccggtca gcacgcattc cacgacggga gccagaaagt gcagtgcctg gctgccgaga
     4381 aagttcatgg ggcccatcga ctcgagaaac aatgtggccg gtgcggccat gccgcgcgaa
     4441 acgacggcgt cggcgacccg ttcgagcagg gccaggtctt cgatcgtgaa ggctgcttgt
     4501 tcaggacgca cggcaaatgc gtgccggaga ccggctcgaa aatcctgtgc gcgacgggat
     4561 aaaaaaccag cggagaaggc agacataagt gcttgggaga tcagcttaaa cggttcgttt
     4621 tggcattata tgaaggggca tcgggagagt caatcgagcc tgtggcgacg cgcgcgacga
     4681 ggggctactg gttcttgttc tctgccgggg gttcgccgga tgccggtgag caattcattc
     4741 cggcgaaggg attcagcggg ttgacgcgtg gtgagcaacg ttgcgcttct ggtggggcct
     4801 tctcgggggc atccttcgca acggccggcg ataacgcagg atcaccggga ccgggttggc
     4861 gaggaaccat cggtgcggcc gaaatggacg gcgggatcgc attcgccggc agttccacct
     4921 gtgccggtac gctcggctcg accggcgggg tgaatcctcc cgggagcggg ggggtggcgg
     4981 tgtcgatggc gacgcaaccc gtcttgccct tcggctggtc tgtcaggact gtgctgccac
     5041 tggcgccttg gcaacggtag accttcgcgg tcgccttgcc cgccgggagc agcacgatga
     5101 tggtgagaag caggacgtga acccagcgca tcgtcaattc tccggtgccg gatcctgtcc
     5161 ggtgaaggct gacggacgaa cgatcagtcg ctgacctgga tggactatag gcgtgaggcg
     5221 gaggagagtc aatctcgccg cggtgcggcg aggggtttgt ggcgaccctc agcccggtga
     5281 gcggccgagc cacttcttca gcaggcccag gagtccgacc tgcggggctg cctgccggtc
     5341 cggatattcc acccacagct cctgcgatcc gaggtacaag ccgtcgcggt agctctgctg
     5401 gctgcgcagc tgggtgtagc cctcttgctg gagggtcgtg atcaattgtt gcagggcctc
     5461 gcctgcgctc ggcgcttccg gggtgacgat gtgcgtcgtt tcataccgca acgtcgcctg
     5521 gtaccccgag gctgtccgca cgaattccac gggacgattg aaccctcgtt ccctggcgtc
     5581 gaaaccggcg agttggtgtt tctctccagc cggggtggac atcgaacgat tagagcgcgc
     5641 gatacacggc taacagatcg ctcaaggcga tggtcttgtt cttgagaatg acccgctcgg
     5701 ctcccagcac atccagcgcg gccacctttt cggttccacc catgagacac ttgctgccca
     5761 atgcgaggac acgttcacac tctttttcca ccttgccctt gacgacgggg ttcaccttca
     5821 ggatctccat catcttggcg cgggtttcgc cgaggtgctt ttggagttct gcttccgggt
     5881 attccttgcg cgcggcttca tcttcgcaac ggtcgatgta ctgcgtcaga aagacgtaga
     5941 gccgcacgaa ggtttcggcg atctcaatgt gtttcggggt cagcgtattg tctgccatcg
     6001 gagcctccat tcactgtggt gccggaaaca ttcattcgct gccgtgacct ggtgaccggc
     6061 gcgggccggt cttgcgtcac agccggactt taatgtcggt gccttcgacc gtgacttcgt
     6121 acgcggtcac tttcgccgag gggttgttga cgcaggtgcc ggttttcacg ttgaactgcc
     6181 agttgtgcca ggggcaggac accacatccc cttccacatc cccttcgccc agcgggccgc
     6241 cccggtggac acaggtattg tcgatagcat aaaaggcgcc atccacattg aacaccgcga
     6301 tggccttacc gttgacttcc gccaccatgc cggctcccgg cttcacgtcg gcggttcctg
     6361 cgattctgat cagctcgccc atacatcctc cctgagtgcg tccagcttcc ttgatcgggc
     6421 gtccggttaa tttcccgtcg ccggtcgttt gattttgtct aacagactgg cgatccggct
     6481 ctttgccgcc cctgcctcgc ccgtttgtaa tgctgccgca agtttcgcgg cgatctcgcg
     6541 aaacgcctga gcttgcggtg aatccggctt ggcgaccacg atcgggttcc cggtatcgcc
     6601 gccggcgcga atggccggat cgatgggcac ccgtccgaga aacggaatgc ccagtttctc
     6661 cgcggcccgt tctccgccgc catgggagaa aatctcggtg cgttcgccgc aatggccgca
     6721 gaggaaaaag ctcatgttct cgacgatgcc gagcaggggc acgttgacct tctggaacat
     6781 catcatgcct ttgcgcacat cgtgcaacgc cacttcctgc ggggtcgtca ccgtgacggc
     6841 gcccgacagg gacaccagct gggtcagcgt cagttgcgcg tcgccggttc ccggcggcag
     6901 gtcgatcagc agataatcca agtcgcccca gaggacatcc cggaagagct gttgaatggc
     6961 ggtgtggacc atcggaccac gccacacgac ggcggtatct tccggcacaa aaaatcccat
     7021 ggagatcagt ttgacgccgt ggctctccgc cggggcgatc ttgccgtctt tctgttcggg
     7081 tgttttttcg acgcccatca tcatcgggat gttggggccg tagatatcgg catccagcaa
     7141 tccgaccttg gctccggtga gggcgagggc gacggccaga ttgaccgaca cggtggattt
     7201 accgacgccg cccttgccgc tgctgatggc gaccacatgt ttcacgccgg gaatgatatt
     7261 gggcttggcg gccgcctctt cttgatgcgg tgcgccacct tgttgctctg ccatagatct
     7321 gcctcctacc acctcgcggg tcggggtgga attcgcgggt ttcattgcat attaagctat
     7381 cgccccgggg aaaagaaatg ggccagtaga cgcagtgcgc agctgggtag aagagggcga
     7441 gatgaaaggg agggacgaaa cgtctcggga cgttcagtga tcagtgctca cggttgaatg
     7501 gagcaggtaa gaacgagaat cgagcatacc tgaacgacgc gagggatgtt tcacctttca
     7561 cgtctcacgc ctgctaggtg cgttccctgt gcaggttata cgtcaggccg aacaacgaca
     7621 acgaatagat caaccatttc gacgggtcga agttgtacca cttcgatccg tttcgatagt
     7681 cgtgcgaata ggtgtggtga tagttgtgat agccctcgcc gaagctgacg aatgagatca
     7741 accagctgtc gcggctgctg tcctgcgtgc cgtggggttg ggtgccgacc atatggcaga
     7801 gggagttgat cgtaaacgtg gagttcagga ccaacacggt ccgcaggacg ccggcgagga
     7861 ggaaacagcc cagcccgctg gtgaagccac cgttcaggta tcccagcgca aatggtagca
     7921 ggaagccgga ggccacgatc acccagtaat accggtgttg ccacatgatc atcgggtcgg
     7981 tacgtagttt cgctgcaaac cggtcctcac gatacggggt atcgaaaaac aaccagccga
     8041 cgtgactgtg ccagaatccc ttcgacgcat tgtagggatc ggcctcttcg tccgtgtgcg
     8101 catggtggcg gatgtgatcg gcgcaccaca tgagggcgga gttctgcacg gcccagcctc
     8161 ccatcaccag gaggcaggct ttcacccagg gatgactctc gaagctgcga tgggtgatca
     8221 tgcggtgata gccgaccgtg atccccatgc cggtcacgat atagaggacg gcgagcagga
     8281 cccaatcgag cagggtgtag tcatagaagt aagcgaaaag cgggacgccg acgaaggtca
     8341 gcagcgtgat tgcagtgaac agggccacgg tgccccagca gacggattgt gtggtcgagt
     8401 cggccatcgt actccttcgg aacctgtgcg caactcatcg ccacagtgca ccgccatgca
     8461 aatggtgtag gtcaaggcaa acggcgcatg ctaccggagc ggcaggcgga ttttcaagca
     8521 cagaagtgtc gcgagatgac acaacggccg attagctggg acgtcctgaa gaaatcgttc
     8581 gccggggcga tacgcatatg ccggttccgc tagttacgta cactcgactc cgcgtgcctg
     8641 tggttgagcc aaatccgtat cccgctgttc ctggcaacga tgcgaaatcc atcctgcgta
     8701 agatggaccg cggcctattc gtcctcaaat attcaagtcg cgcgctgcgg tgtatactgc
     8761 agagagcctc tcattttggt tcaggaggaa ccgatgcctc tcgaccctgc cccgctcgaa
     8821 ccccgtatcc gcgcgattgg tgaagacttg gcccggcgtt ccggcgggcg ggcgcccggc
     8881 ctgttcgatt cgcgctggtg gtcgcaggcg gccatcaacc tggcgatgaa agatcccgcg
     8941 ttcaaggcgc aactgttccg gtttatcgat gtcctgccct cgttgcacga cgatgcacgg
     9001 gtcgtgcagt tggccgagga atatttcggc gagatgagcg cacacctcgt cggtgcccaa
     9061 tggggactca aggcgcttgc ctccaccaag ctgggcgcat cgctcagtgg aaaggccatc
     9121 cggcatcagg tggagcagat ggccaccagt ttcatcgccg gtgcgtccat cgagcaggcc
     9181 gttccgacgc tccgtaagtt atgggaggac gggcgggcct tttcggtgga tctactaggt
     9241 gaagccagtg tcagcgagcg agaggcggat gcctatcgtg atcgttgcct cgcggctttg
     9301 caggccctcg gtgaggcctg tcccggttgg tcccctgcgc cgttactcga acgggatcac
     9361 cttggtcccc tgccccgcgt gcaattgtcg atcaagatct cggccctcta ttcgcagctc
     9421 gatcccatcg atcccgaatc cagctatcgc gcagtcgctg cccgcctgcg gccgttgttg
     9481 aatcttgccg gcacgctccc cgcctcggtc attttcgata tggaacaggc ggacaccaag
     9541 gagctgattc tttcgatttt catgcgcctg tttatggaag aaccctaccg aacgtttccc
     9601 catgcaggga ttgcgctgca ggcctatcgc accgatacgt atgacgatgt ccagcggctg
     9661 ttgacttgga gcagggagcg gctggtaccc gtcacgatca ggctcgtgaa gggcgcttat
     9721 tgggactccg acgcgattcg ttatcggcaa cgtggctggc cgatgccgct gttcgaacgc
     9781 aagacacaga cggatgtgaa ctatgagagg ctcgttcgat tgctgctgga gcagagccat
     9841 gtgatccggc cggcgttcgg cacgcacaac cttcgaagtc tggctgtggt cgaggcggtg
     9901 gccgaggcgc tgaagctaca gtctcaggcc tgggaatatc agatgatcta cggcatggcc
     9961 gagccgtttc agcatgcgat gagtgatcgt ggccggcggc tccggctcta tgctccggtc
    10021 ggagaactcc ttcccggcat ggcctatctg gtgcggcggt tgttggagaa cacgtcgaac
    10081 gaatcgttcc tgcggaagga atatgtggag tcgcagcctc tgtctttgtt gctgtctccg
    10141 cccgatgcga ccctcccctc gcctccttcc ccgatggcgc ctgaggccgg cgcttcttcc
    10201 accgtgggga gcgctcactt tgtgaacgaa ccggcggcgg atttttcacg agacgacgtt
    10261 cgggtcgcca tggccgaggc catcgatcag gtgcgcaaac aattggggca gcggcttgag
    10321 gtctcgaggc tgaatacgca tctcccgacg gggccggatc tacattcctt caaccccggt
    10381 cagccggaac aactggtcgg catcgtgcag agttatctgc ctgccgacgt ggctggtctc
    10441 ttgaaggtcg ctgagtctca tgccgaggaa tggaaacatc gtgcggttgc ggaacgggtc
    10501 gccgtgatgc ggaaggccgc ttcgttgatg cgcgagcaac gttgggagtt ggcggcctgg
    10561 gaagtgttcg aggaggccaa gccctggcga gaggccgatg ccgatgtggc cgaggcggtc
    10621 gattttctgg agtactacgc cggagaaatg gaacagcttg atccgccgcc ccgcttgggc
    10681 cggtatcccg gtgagttgaa cgagttgttc tggagtccgc gcggagtcac ggttgtcatc
    10741 gcgccctgga attttccgct cgccattcct gccggtatgg tcgcggcggc tctggtctcc
    10801 ggaaatccgg tcttgttcaa accgtctgag cgcgcgtcga tcatgggcta tcggctcgcc
    10861 acgattctga ggcaggccgg ggtcccggcg ggcgtcctgc agtacgtgcc gggtggtccc
    10921 gacgtggggc gtgaactggt gactcaggcg tctgtcgcga cgattgcctt taccggatcg
    10981 aaggatgtgg gattgggcat ggtgcaggcc gctgcggttc aacaaccggg acagaagttc
    11041 gtcaaacgcg tcatcgcgga gatgggcggc aaaaatgcca tcatcgtcga tgacacggcg
    11101 gatctcgatg aagccgtgac cggtgtggtg caatcgtgta ccggttatgc gggacagaaa
    11161 tgttccgcct gttcacgtgt gatcctctta gattcgattc acgatgcgtt catccaacga
    11221 ctggccgatg cggtgatgag tctgcgcatc ggcgcggctg aggatccggc tacccaggtt
    11281 ggtccggtga tcgacggacg ggctcagaaa cggattcggg actatctgga gatcgggcga
    11341 cgcgaaggcc gggtggtggt cgatcggtcg atggagggcc ccggtcatgc cgtgggaccg
    11401 gtagtcatcg cagggatcga gccgactcac cggctggcac aggaagaaat tttcggcccc
    11461 gtgctggccg tgatgcgggc ttcatcgttc gaacaggcgc tccagctcgc caacggcacc
    11521 gcctacgcat tgaccggggg actctactcg cgcagtcccc ggcatatcga gctggcgcgt
    11581 caggcgttcg atgtcggcaa cctctacatc aaccgttcga ttaccggggc cctggtcggt
    11641 cggcaaccgt tcggcggtca ccggctatcg ggtatagggg cgaaggccgg gggcgaagag
    11701 tacctgaaac aattcatggt cgcgcgtgtc gtcagcgagc agacgctccg gcgaggattc
    11761 gctccgaccc tgtagtgccg gcgcggattt tacggtgacc tcttccagtt cttccgtgat
    11821 atcggtggtg ttgccccggt cttccttgag cgcggccaat tcctgccgtt cgatatattt
    11881 caccagcccc actcccttgg cgaaccaggc cgtcatcaca tcggtgccga ccgccgtccg
    11941 atcggcggag gagagatgaa tcttgagggt catccgcgct tctacgcgca cggcgtcggg
    12001 atacgtgccg gcgggcaccg tgatcgattc ctggccgatc accgtcgaaa ctccttcgac
    12061 gtccacatgt tcgttggtgc cgtccccatc gatgtcactg ccgaaatcga tgtccatgcg
    12121 attgaactgt tggaaggatt gggccacttt catcgggaac cggatgatct gatacggcac
    12181 gagctgcttc tctaacggcg tgcccggctc ggagccgtag tagaccacgc ctgccgcatc
    12241 gcggcggtaa aagctgtcgg atgcgccatg gttgcctggg ttggtgtcat gaaacacgtt
    12301 gacgaccacg ccgcccttga gagtgcgggt tccggtcacc gacgacacgt tgaagaagta
    12361 tttgttctcg atcgtttgga ggggcccttc cgagatctgt ccgcggtact gccagcgtgt
    12421 gccgacggtg tcggggaaat agtcggcaga ctgggcgatg gtttgtcctg cctccgttgc
    12481 ccgggcctgc ccaggagccg acgggatgcc caggaacccg cacatgccca ctgccatcac
    12541 ggtcgagcgc accaaggtct gaacgatcac ggctgtcctc cagtcaaaaa aatcacggta
    12601 ccatacccga ttcgccgcag caagccggca ggtccagcct tgccttctcc tgtgcctcct
    12661 ctcataatcg cacgccgtga tgtccgcatt ccctcccgca gctcaagatg ttcgtgaacg
    12721 ccggacggta ctggtgaccg gtgggtccgg cggaatcggt cgtgcgctct gtctggcctt
    12781 cgcccatgcc gggtattggg tgggagtgca ttacttccgc caacggccgg cagccgaaca
    12841 aaccctcgcg ctgctctcgc aggccggtgg tcagggtgcc ctctaccagg cggatgtacg
    12901 gtcgagccat gaggtccagg cgatgctgga tgcgatccgg gcgcagcgcg gccggctcga
    12961 caccctgctc tgtaatgccg gcatcgcgtc gaagcatctg gtggtgaatt gcccggaaga
    13021 agcatggcaa cagatcatcg acacgaatct gacaggcacc tatcgctgca tgaccgccgc
    13081 ggtgaacgac atgatgccgc agggcggtgg gtccatcgtc gtcatcgggt cctttgccgg
    13141 attgcaggga acgaacggcc aagccgccta tgcagcgtcg aaggcggggc tgatcggctt
    13201 ggtgaagacc gcggcgcggg aatggggacc gcttaatatt cgcgtgaata tggtctgtcc
    13261 cggctggcag caaacgcgca tggccggtga ctcgtttcca accggtgatc ggctcgacga
    13321 ccatgtgctg ggacgtgtct cgaatctggc tgatgtaagc aggacgattt gccaactcgc
    13381 gcagctctcc gatatgtcgg ggcaagtgtg gaacctggac agtcgcattc tctagcagga
    13441 tgctgaaaac ggcccccagc ttcgttctcg gtcgtacgtc tccttgcgac attggaagta
    13501 taagagggag tactgaaggg gtctatccgt tcaccaagag tatctgattg gtgaacgggt
    13561 cgacaaagtt tggtgtgtac ctcctcagtc gtcgtactcc ctgcggcctt gctggacgcc
    13621 gttttgagca tcctgttggg aaattgagtc gtcgatttca cagcacgaaa cagtgtgttt
    13681 cacgggtggt aaatgggaag aacagagaat gggtaacggg atatttgtca ccggtacgga
    13741 taccggcgtt ggaaagaccc tcgtgtcctc cgcattggtc cggannnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnaccggt acggataccg gcgttggaaa gaccctcgtg
    13921 tcctccgcat tggtccggag gctggtccag actggccgct ctgtcggggt gatgaagccg
    13981 gtcgaaaccg gggtgcttcc cgagcaactg agtgagagcg acgcggggcg actcatggct
    14041 gcggcgggag tatccgatgg gatcgatgag gtgtctccct accggttccc cgcgccgctc
    14101 gccccctttg ccgccgcatc cgcgcaagga agtgtaatcg agatgcagga gatcatgaga
    14161 cgctacgaac ggcttgcggc ccgggactct tgtgttgtgg ttgaaggcgc cggtggtctg
    14221 ctcgtgccga tggggaagga ttgggatatg cgcgacttga tcggccggat cgggctgccc
    14281 gtcgtacttg tggggcgggc cgggttaggt gggatcaatc atgcgctgct gacgctggag
    14341 ggccttcggc aacgtcactg tgcggtgatg gctgtcgtgt tgaacgagac ggcgttgtcc
    14401 accacccccg cgcagcagga gcagtgtgcg tctacggtcg cgctgttgcg ggagcgagcc
    14461 ggtgttccag tgttgggacc gctgccctat cagccacaac ttggcactga ttggtccggc
    14521 gccgtcgagg cgctggcaca gagcagcccg atcagggagc ttgcgaatct ggtgtgggca
    14581 aatgctgttg gaatgtccgg atcgccgcgc cgaggtccgg agccttgagg atcgcagaga
    14641 tcaccgccac gccatccgcc cctgccgcca taatttcacc aaccgactca gccgtaatgc
    14701 cgccgatcgc aaacatcggc agcgtggtca acgcgcgcgc cgcgcgtaat ccttccagac
    14761 cgaccaccgg gtcgtgatcc gcttttgtgg acgtactgaa gatcgggccg aaccccacgt
    14821 agtcgggttg tgcggtgacg gcttcgcgca cctgagctgc attgtgcgtc gagagtccga
    14881 tgatttttcc cggccccatg aggcgtctgg cgtaggagat gggaaggtcg tcctgcccca
    14941 ggtgcacccc gtccgcgttc acggccatcg ccaaatcgca acgatcgttg acgatcaaga
    15001 gggcgccggc gtcggttgcg gcctgacgca gcgcgagcgc cttcgcatag gcgtctctca
    15061 tcgtggcctg cttgtctcga tattgaaaga gccggacacc gtgcgcggct gcttcgcgca
    15121 ggagatcggg cagcgggcgg tccggccgga ccgtggggtc gagcacgagn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tctgtttgtc agcaaactgt tacgaggact
    15301 ggccggcaag aaagcggtcg aggaacgtcg tggagacgtg tcccttctgg aaatcgggat
    15361 cgttgaaaat tttccgatgg agcgggatcg tcgtcttgat gccttcgatg acaaattcat
    15421 cgagtgcgcg gcgcatgcgg gccatggctt cctggcgatc acgtccgtgc gtgatcaatt
    15481 tggcgatcag gctgtcgtag tacgggacga ccacggcgtt cggttccatc gcggaatcga
    15541 cgcgcacgcc aaagccgcca ggggcggaat atttggtgat ctgccctgga cagggcgtga
    15601 acttttccgg gtcttcggca ttgatccggc actcgaagct gtgaccgttc aacttgatgt
    15661 ccggctgctt gaacgagagg ggcatgcccg aggcaatccg gatctgttct ttcacgaggt
    15721 cgatcccggt caccatttcc gtgatggggt gttcgacctg gatgcgggtg ttcacttcca
    15781 tgaaaaagaa gttgcgatcc ttgtcgagga gaaactccac tgtgccgacg ttgcaatagc
    15841 gaatggcttt cacggcctgg acggccaccc ggccgatttc gcgccgtaat ccttcatcga
    15901 tggcaggcga cggggtttct tccaccaact tttggtgacg tcgttgaatg gagcaatcac
    15961 gctcgccgag gtggaccaca tggccccgat tgtcggccac gatttgtact tcaatgtggc
    16021 gcggttcgag gaaatagcgc tcgagataga cgccgtcgtg gccgaaggtg gatttcgcct
    16081 cggcttgcgc cgcttgaaac gctcgcgcga gctcctccgg tttgttcacc acgcgcatgc
    16141 cccgtccgcc gcctccggcc gaggccttga tgatcacggg gaagccgatg gtcttggcgg
    16201 cctcttgcgc atcctgttcg ctccggagtt cgccggggct gccgggggtg accgggaggc
    16261 ctttcttggc gaccacttcc cgtgcctttg atttgtcgcc catcagcgcg atgttttcgg
    16321 atgtcgggcc gatgaatttg acgccgatcg attcgcagac ttccgcgaag tgcgcgttct
    16381 ctgagaggaa tccatagccg gggtgaatgg catcggcccc ggtgacttcg gctgcgctca
    16441 gcacgttggg gatattgcgg taactgaggg cggcttccgg cggtccgacg cagacgtgct
    16501 cgtccgcagc ccgcacgtgc aagcttgcgg catcggcttc ggagtggatc gcaacggttt
    16561 tgatgccgag ctctttgcag gcgcggatca cccgaagggc gatttctcct cgattggcaa
    16621 ccaacacttt cttaaacaca ggatactccg gcgcgggtca ctctctgtgt ccgcctggtt
    16681 ctaaacggtt tgctctgcca cgggtgagac ctgatccggt tctgttgcag gatcccggca
    16741 tgtgccgtct gccgtcggac ccacatgctt gaggaacgta tccccggatc gtctcgccat
    16801 gacccgcatg atctcgtcgg ctagggcgtg gcgttggggt ctatcaagaa gagaggttgt
    16861 ccgtactcga caggcttggt gctctccgcc agtattttcg tgatccgtcc gtcggcctcg
    16921 gactcgattt cgttcataag cttcatggct tcgacgatac aaagcacttg gcccttcctc
    16981 acgtaatccc cctcttccac atatgggtct gcatccggtg agggggagcg atagaaggtg
    17041 ccgacgatag gagacgtaat cgtaacctgg ccatccgttt ctgcgggtgg acgggtctgc
    17101 gccgccggcg tcgctccggt ggaggtcgac tggttcgtgg cggtctccac cgtctgggcc
    17161 gtcgtggtcc ggattaccgg ctcatgtctg atgcggatac ggactccgct gcgctcgatt
    17221 tccaactcgg tcagatggtt gcgcttgagc aggtcggcca actgttgaat ctgcgcggcc
    17281 tggtccggag agagcagtgt ctcagcggcc gttgatcgag gcgcaaaggc gctcggcagg
    17341 acgatcggct tcccggcccc ccggcctttt cctgtctttt tacctgatga gctcaacgca
    17401 cgcgctccac aaattcacgg gttctcgtat cgatgcggat cgtttctccg atttccaggt
    17461 agagcggcac cttgatcgtg gcaccggtct cgacgatcac cggcttattg ccgcccgagg
    17521 ccgtatcgcc gcgcacgccc ggttcaccgt cgaccacttt gagttcgatg aagttcggca
    17581 gcaggaccgt aatcggccgg tgctcgtaga tcaggatctt ggcgatcatg ttttctttga
    17641 gcagatcggc gttgcttccc aactggctct tctcataggt cgactgctca tacgtctccg
    17701 tatccatgaa cgtgtaggaa tcgttcgtgg catacaggaa ttgcatgtcg cattcttcga
    17761 tctggggtgt gtcgaatttt tctccggaac gaaaggtcct gtcgaggacg ttgcctgaga
    17821 gataactctt cagcttcgtc cggacgaatg ccccgccctt gccgggtttg acgtgctgaa
    17881 attccacgat ataaaagggt tgaccttcta ccattaacgg gctgccgttc cgaaactctg
    17941 cggtcgaaat cacaacaact ctccttggta caacaagttc aacggcgcga agccgtgggt
    18001 ttattcttcg gggccgtccg agggcgtgat gccttgctga cggcctcgag gtgcgcggcc
    18061 aatgcgtcca gcgccagcag atagccggtg ggaccgaatc cggaaatctg tcccagggcg
    18121 acgccggcga tgaaggagtg atggcgaaac tcctcccgct gatggatgtt cgacaaatgc
    18181 acctcgaccg tcggtagggc gacggcggcg atcgcatcgc gaatcgcgat gctggtatgg
    18241 gtatacgcgg ccggattgat gatgatgccg tcgaaatggc ccctggcgtt ctgaatccag
    18301 gtgaccagtt ccccttctac attcgtctgt ttggtctgca ccgcaatccc ccgctcgcct
    18361 gcgcgacgga caatcgactt gtcgatgtcg ggcaaggacg cctgtccgta gacggatggc
    18421 tctcgcgttc ccaggagatt gagattggga ccatgaagca ctagcagccg cagcatgtgt
    18481 ctgtcgcgcg cccccgttat cagtaggctg caatggatag agatgccagc agcggcggca
    18541 ttctaacagg gtgtcaggag tgggtcaaac gttgttcaga gggcgggacg agcgtgggtc
    18601 cgcctggggg gggcagtcaa tgaacgcgtt gggcttgaat ggttcgcagc caggcttcca
    18661 gtcgcgcgat cgcggccgca tgtttcacga caggcagatc cggggtcggc cggcccggtg
    18721 tttcagtgac cggctcagat gccatagatg acgtggatac ggttggctcg ggagcgggcg
    18781 tgatgtgagc cccagctttt gtgccgtcgg gtctaggctc cgtgacgatt cgtttcttct
    18841 cgcgtttggc ggccggagga ttgtcaggtg ctccggtgat gctgcgtttg actgaggccg
    18901 cctcttcatc gaacgggttg gcgttcagga tggctgtgca ggacagcaac gctaagtcgg
    18961 cgttcccttc ggcgtgatag agcttggcga gaatacgatg cgcgcgaaga ttgtccgggc
    19021 gctgtctgac gacgtcttca agaatggcct tcgctttcac cgcctgccct aattgatcgt
    19081 acacccggcc caatgccgcc atggcggtga caaagccggg atacaccttg aggccgtctt
    19141 caagtacgcc cgctgcttcc tgccacatgc cgaccttgat gtattcgtct gcgaggggga
    19201 ggaattcttt cgagcgcggg tcttttgcga cggcggtggc caggcggtcg atttcggcgg
    19261 cggtcgatgg atcgatacgc gatgaagaag ccacgcccta ctccttgcga tgtgtccctg
    19321 ttcctgcggc tggcttcgtc gaccttcggc cggtcggtag ggtcaggacc cggtccaaaa
    19381 tgcggtctgc gacctcacgc ttgggtaaca gggggagttc ctccgcgcgg ccgtcacgat
    19441 ccaggatgaa cacgcgattc gtgtccgacc cgaatcccga tcctgctgcc gtcacgtcat
    19501 tggcgaccag caggtcgacg tccttagctt gcagcttttc ccgcgcatga gcaagcagat
    19561 cctcagtttc cgccgcaaac cccacgagca cctgtgtcgt ccggcgctcg cccaattccc
    19621 tgagaatatc gtcggtcggc gacaactcca ggctggtgac gggactccgt cgctttttga
    19681 gtttctgtgc agcgggttgt gtcgggcgaa agtccgccac ggcggcagcc atgatgacgg
    19741 tatcgctcca ggaaaaccgg ctgatgacag ccttgcgcat ctcctcagcc gttgtcactg
    19801 gacacaactc cacgccggcc ggcggggtca gggccgtggg gcctgagacc agtaggacct
    19861 ccgcgccgcg tgcctgcgcg gcttcggcga gcgcatatcc catctttcca gaggaccggt
    19921 tggaaatgaa ccgcacgggg tcgatggctt cctgcgtagg gccggcggaa atgagcagac
    19981 ggcgaccgga ccaatcccga cgcgggtgga gcgtggcctg gagtaccgaa aggattcgtg
    20041 cttcatcggc cagccgtccc tgccctgaac ggcctgaggc cagcgggccc tcttctggtt
    20101 cgacgatgac tgcgccgcgt gttcgcagcg ttgccacgtg ctccacgacg gtcgggtgtt
    20161 gccacatatc cccgtccatg gccggcgcga aaatcacggg acattgtgta gtgaggagca
    20221 tggtcgagag gagatcgtcg gcaagcccga gcgcggcttt ggccagacaa ttggctgtag
    20281 ccggggcaat caggatagcc ttcgcctgct ccggcaggga gaggtgcagc atttcctggt
    20341 gggcttcgaa gaggtcggtg gccacgtggg aaccggacaa cacttcgaag gtcagcgggg
    20401 tcacgaaccg gcaggcccct gccgtcatga cgacgttcac cgtcgcgcct tcgcgacgga
    20461 gcaggcgcaa gagcccgact gccttatagg cggctatact ccccgtgacg gcgagcacaa
    20521 ggtggacacc ggtgagtgag gggccggtgg tctgcacgaa gcgggactac tcctcgccct
    20581 ccggagcagg ggccggcttg acggtatcgt cgacgtagac gctcagctct ttcttgattt
    20641 ccttggcgtc ctctccggtc atcatggcca ggcgttccgt ctcaccttcc ttgacccgtt
    20701 tggattcttt aatggcctcg cgggcttcct gtccgatcag gaatttgacc ttgtgccgaa
    20761 ggacttcgtc gagcgcgatg ctcgtttctt tggtgaattt ggacgggccg gacatgcgcg
    20821 cgccctgcac gagttgtttc gcccgctgcg cggcgataat caccagccgg tggcgtgaat
    20881 caaactcccc ggtggcgtat tccggtaaca aacttaacat ttcacccatg atgcaagtgc
    20941 tcctttgatg cgggggcgga ggaggaattc ccctgcttgc cctctttgtc gagaatgaat
    21001 ttttcttcca accacgtcat gttcaggcgt ttggttttga tccgttccgc caggaaaatg
    21061 ctttcgagct ctttcaagga ttgcttcagg tcgtcgttcc ggacgatgta gtagtactcc
    21121 cggtagctcc agacctcttc tttggctttc tgcaggcggc gttgaatttc gtccggcgag
    21181 tccccagccc ggttttgaag ccttgtccgc agcgtgtcga acgacggcgg cagaatgaag
    21241 atatacacgc cgtcttcaaa cttttttttc actgagcgcg cgccctgcac atcgatttcg
    21301 agcagcacgt cgatgccctg ttccattttc tcggtcagca tcttgcgcgg tgtgccgtac
    21361 aaattgccgt agacatgggc ccattcgacg aattcattcc gatcgatcat ctggcggaag
    21421 gccggctcat cgagaaaatg gtaatcctgc ccatccacct cgcccgggcg cggtttgcgt
    21481 gtggtgtacg agatcgaatg ccacaatcca gggacattgg ccgtgatctg tttgcacaat
    21541 gtcgtcttcc cgctccccga cggagccgaa atgataaaca aaatgcctcg ccgtacttgg
    21601 ggcggtttat cgttggatga aacgggcgcg gtattcatag ggtgttcgat gccggcgcag
    21661 ggccgacact attcgacatt ctgaacttgt tcgcgcaatt tttccagttc ggctttcatt
    21721 cggaccacca acgcggcgat gtcggcgtcg ttggcctttg agccgatggt gttcacttcc
    21781 cgccccattt cctggagcag gaactctaag gtcttcccca ccgactcctt actgtggagc
    21841 tgctggtcga actgtagcat atgtgactca agtctgacca attcttctga gatgtccgaa
    21901 cggtcggcaa acaccgctaa ttcctgttgg agccgggccg gatccggcaa ttcggcctgc
    21961 agcagggtct caatccggcc tttcatgcgc tcaaatgcat gcttggccag taccggggcc
    22021 ttttccgcca cgagggactt ggcctcacgg atcgcatcca gatggagagc caggtctttg
    22081 gcgagtgcct ccccttcccg tcggcgcatc ttctcaaggt ctgtgagcgc gcctcccagt
    22141 acccgcagca cggttttcgt cagttgcccg gcatcgaccg gctcatcgga aatagacagg
    22201 atgtcccgga atcccgcgag cgtggaaaca tccaccgctc cccgcagccc cagtgttttc
    22261 tgcagcttct tgagggccgt atgatactgt ttggccaacg cctgatcgat ctggaccgtc
    22321 ttcaggctgc cgccggcgga atggaccgag accgacacgt cgactcgtcc gcggaggcag
    22381 cgttgctgga cggtttttcg aacctggtcc tccagttgcg ccaacgaccg gggcagtctg
    22441 actgccactt cgagaaagcg gtgattgacc gaacggatct ccacggtgac gccggctttc
    22501 tgtgacgtgc cgtccttttt cccatacccc gtcatgcttc gaatcatcgt gcggttttcc
    22561 ccttccaatg gcaatcggca tagatcggac aatgctgaca tttcggtgtg cgcgcgaggc
    22621 agatgtatcg gccatgcaga aggagccgtt gggatccttc cgtccactgg tctgccggca
    22681 gtaagcgttg caggtccagc tcaatctttt cgggatccgt atggcgggtc aggtcgagcc
    22741 gtccggccac tcgtttcaca tgtgtgtcga ccacgatggc cggcttctcg aatgcgttgc
    22801 caagaaggac attcgccgtc tttcgcccga ctcccggtag cgtggtgagt gcttccatcg
    22861 tatcgggaac ctctccctgg aactgcccgg ccacggcctt cgcacagtgg atgaggttcc
    22921 tggcttttgt tttgaagaag ccggtcggcc ggatgagggc ttccaattcc gtgggatcgg
    22981 ccgaagcgta atcgcgaggc tgctgatagc gacggaacag attcggggtg acctgattca
    23041 cccgctgatc ggtgcattgc gccgacagga tcgtggcgac cagtaactcc cagggcgtgc
    23101 gatggtcgag ctcgactttc atcgccggtg aggtcgcgcg taatgcggtc aacatccggg
    23161 cgaggcggcg tttggttgtc acgccggccg gttgatctgt tgtggcgact ttcatgatgc
    23221 ggtgtggtgg cggccaattg tgcagaggtc aggagtgaga tgcaagcgcc aggtcctgag
    23281 gcggctggtc aggatcttcc tcgtatcccg ggggtgtcgg aatggcgggc aagagggatt
    23341 caaggtgggc gatcaacggg cgcagcgtct cacggttcgg cgccgagata ccgatcgcgt
    23401 gataccgctg gcagaggacg ccggcctcat gtgccgagag gcgatcacac ttgttcagga
    23461 ccatgacgcg gggaatcgtg tcgaggttga gcgattgcaa gacggtttcg acggcggtga
    23521 tctgttgatc gacgttgggt gccgacgcat cgaccacatg caggagtatg tcggcatccc
    23581 gcaattcgtc cagcgtggtg cgaaaggctc ccaccagatc tttggggaga tcccggatga
    23641 accccaccgt atcggtcacg atgacttcgc ggtcatgagg aaaacgaagg cggcggctcg
    23701 tcgtgtcgag ggtttcaaag agacgatcct gggccgggat ctgactgtgg gtcagggtgt
    23761 tgagcagcgt cgatttgcct gcattggtgt agccgacgat ggagaggatc ggaagcccct
    23821 ggcgcacacg tcgggatcgg cgctgatctt gatggcgggc cacgtcgtcg atctcccgct
    23881 ccaggtgcgc gatgcgatca cggatgcggc ggcgatcggt ttccagtttg gtctcaccgg
    23941 ggccgcgaga accgattccg cctccaaggc gcgacaacga ggtgccatgc ccggacagtc
    24001 gcggcagaag atatcgaagc tgagccagct ccacttgaac tttgccttca cgggtatggg
    24061 cgcgcccggc gaaaatatcc agaatgagct gcgtgcgatc gatcactttc agatccgtca
    24121 cctcggaaat ggcgcgggct tgcgccgggg cgagatcctg gtcgaagatg acgaggtcgg
    24181 ctcccttctg gagcgcgcgc atcacaacct ctttcagttt gcccttcccg agcaagtatc
    24241 gctcgtaccc ttcgtgagtg cgctgggcga ttcgatccag cacgcggacc ccggcggact
    24301 cagccaattc attcagctcc tctagatgct cttcttgttc cgcgtgactc ttgggtgccg
    24361 cgctgacaag aatggccgat tcggtgccgg cggcgacgtc gtgcttggta tccgatcgct
    24421 gcaaatcgct ttcgagcgca tggaggaatg tgtccagttg aaggtccaga tcgtggaacg
    24481 ggaccggttt caggatgcgg cagacttctc cctgttggtt gggtgggagc agatgcgcca
    24541 gatgcagcag gctggggtcg cctttttcgt ctacgcccag gatcccgatc agatccaatc
    24601 gcaggagcgc caggtgggtc aaatcgtcct ggctcaacgg gtcatcgtgg agatgcgtgc
    24661 gaatcagccg gagaccgcgg agggaatgtg atccaactct gaacttcgcc agcgactcga
    24721 aggtgggtgc gcagccggtt cccacgagca cctgttcgat gaccccgcgg cgagtgatca
    24781 ggagaccgat gggacggcga atctcacgtg tcaactggct ggtcgtgcgc gccagttcgg
    24841 tcgtgaggac ggctgaggac ggcacacggc gtcgatacaa tcgctccaat gccgcgaggt
    24901 gactggcttt cagcccggtg acgtgtccgt gaatcgcggg gatggtgatg ctccttggta
    24961 ctcgaacgtt aggatctgcg acgcatcgtt tcgccgcccg acgatgccgg taacgcataa
    25021 ttcggcatcg cctcgcactc gaaggatgcc agcctgccct gctccagagc ccgttgccct
    25081 gccgccgcaa tcatggccgc attgtctgtg cagtatgaga gggccgggag tcccaattca
    25141 atcccctccc cagctgcccg ctccgtcaat cgtgctcgca gcctggaatt ggccgagacg
    25201 ccgccgacga cggcgagggc tgagacgcct gcctgtcgca cggccgcaaa tgtctgacgg
    25261 accaacacgt ccacgatcgc ttcctggtac ccggcggcca ggtcggcctg ttgtcggacg
    25321 atggcttcct gatccatgcg ctggaggtga tagagcaagg ccgtcttgag accgctgaaa
    25381 ctgaaatcca gttgtccggt tctcacgcgc gatcgaggaa atcgcacggc tgccggactg
    25441 ccttttctgg ccgcggcgtc gatgagcggt ccacccgggt atccgagccc gagcatttgt
    25501 gcgcccttgt cgaatgcttc gcctgcagca tcgtcgatcg ttcgtcccat caggtggaag
    25561 ccgccatgtt ccgttgcgtg gaagagatgc gtgtgcccgc ctgaggcgat caacactacg
    25621 caggttctgg ggaatgacgg gcggtcgatc caggctgagg cgatgtggcc ttcgaggtga
    25681 ctcacgccga ccagtggaat gccaagcgcg taggctaagg acttggcata actgataccc
    25741 accagcaggg cgccggccaa tccgggaccc tgggtgacgg cgatggcgcc gagatcctgc
    25801 caggtgagac ctgctgcgtc cagagcctga cgtgtgacgt gatccacatt ctgaatatga
    25861 gcacgtgagg ccaattccgg cactaccccg ccgaaccgtt cgtggacggt ttgctgggaa
    25921 gaaatcacgt tggccaggac atgcccttca ccgtcgagca ccgctgcggc agtctcgtcg
    25981 caggatgttt cgatgccgag aatcgtcgtg gatgcaggag gcttcataga gaaaactgcc
    26041 gacagggtga cggcatgtga acctggttat gcatgcgcgc gccattccaa ggggcgagat
    26101 cagtcgtagt gggcgcacac tgtgactggt gctgttcgta aggaaatccg gacaacaact
    26161 gatcctgctg taaaatgcga caacccccgg atccggttgc ggaccgcggg ggttgccggg
    26221 tggtctctag gatctcaacg acgagtcgat cctctcgtaa tggtcttcgc tcgcgctaga
    26281 cgcctgccat agcctcttgt tcgatgaagg ccggcgcacc gtcgcgcagc acgactttga
    26341 tcttcctgtt ttccttgaag cgacccttga tgagttcttc agacagggga tcaccgatcg
    26401 cccgctggat gatgcggcgc atcgggcgcg caccgtagag cggctcgtat ccttccttga
    26461 tgagccactg cttcacctcg tcgtccacct cgatgctgac gctcttgtcg atcaggcgga
    26521 gattcagttc gtgcagcagg atatccagga tgtggatcag gtgctccttc tccagggaat
    26581 ggaagatcac gatttcgtcg atccggttca aaaactccgg gctgaacgaa cgcttgagtt
    26641 ctcccatcac gtcttctttt cggcgcaagg cctgtccgcc cttttcatca ttctggaatc
    26701 cgagcgacac gcctttttgg atcatcttcg tgccgatgtt cgaggtcatg atgacgaccg
    26761 tgttcttgaa atcgactttt cgacccaggc tgtccgtgag gacaccgtcg tcgagcacct
    26821 gcagcaggac gttaaacacg tccggatgcg ctttctcgat ttcgtcgaac agcacgaccg
    26881 aatagggacg acgtcgaacc ttttccgtga gctgtccgcc ttcctcgtaa ccgacgtatc
    26941 ccggaggggc tccgaagagg cgtgaactcg tgaacttttc ctgatactcg gacatatcga
    27001 tccggatgag cgcgtcttcg ctgttgaaga ggaactccgc cagcgtcctc gccaattccg
    27061 tctttccgac accggtcggg ccgaggaaaa tgaaggaccc gatgggtttc ttcgcttcct
    27121 tgagacccgc tctggatcga cgaatggcac gcgccactgc cgaaatagcc tcgttctgtc
    27181 cgatgacccg cttgtggagg aattcttcca tgcggagcag tttattggat tcttcttctt
    27241 ccaacttgaa gagcggaatg ccggtcatct tcgacacgac atacgcgacc tcttccttgc
    27301 cgatggtcgg cttgtgtttc tcctggttct tcttccactc ccgcttggat tcatcgagga
    27361 gctttcgcag gcgctcctct tcttcgcgat ggcggacggc ttcctcgaag ttttgcatcg
    27421 agatcgccag ctctttatcg cgagaaattt tcttgagctc ctgctccagc gctttgagtt
    27481 cagtcgggag cgcgtacgtt tgcaacttcg cccgcgagcc ggtttcgtcg atcagatcga
    27541 tcgccttgtc cggcaggaac cggtcggtaa tatagcgatc cgccaacttg acggcttcca
    27601 cgatggcgtc ttccgtaatt tccaccccat ggtgttcctc gtaccggtcc cgcaagccct
    27661 ggatgatccg caccgtttca tccgtgctgg ggggctgtac gtggatgggt tggaaccgac
    27721 gtttgagcgc gccgtccttc tcgatgtgtt tacgatattc gtcgagcgtc gtggcgccga
    27781 tgcattgaat ctcgccgcgg gacaacgccg gcttgagcat gttggaggca tcgatggatc
    27841 cttctgccgc ccctgcccca accaacgtgt gcaactcgtc gatgaagata atgatattcc
    27901 cggcctgcac aatctccttc atcacgacct tgaggcgctc ttcaaactgg ccgcggtatt
    27961 tcgtgccggc caccagtgag cccaggtcga gcgcgatgac gcgacgggac agcagattgt
    28021 ccgggacttc cgacgcgatg atacgctggg cgagtccctc gacgatggct gttttcccca
    28081 cccccgattc accgatgagt acgggattgt ttttcgaccg tcggctgaga atctgcagga
    28141 cacgctcgat ttcgtccgcg cggccgatga cgggatccag ctgcccctcc tgggccagct
    28201 gggtcaggtc gcgtccgaat tcgtcgaggg ccggcgtatt gctcttgcgg tcccgctcac
    28261 gcggcgcaga cttacgcagg aaggtgacgg tgagttgccg ggcggtcagg aggttggcgc
    28321 caagactccg gagaatcttc ccgccgatgc cttcttcctc acgaaggaga ccgagcaaca
    28381 gatgttcgct gccgatgtgg ttatgaccga gcaaccgggc ttcttcaacc ccgtactcga
    28441 tgactttctt gacccgcggg ctgaaaggaa tttccccgaa ggtcatcgtt gtgcccccgc
    28501 ccgggagatt ccgctcgatt tcgagtcgaa tctgctcggt ggagagcccc attttcttga
    28561 ggatcatcag cgcaatccca tccgtctcgc gaaggatggc caacaccagg tgctcggtgc
    28621 ccaggtaatc gttctgatgg cgctcggctt cttcacgtgc caggatgatg atcttgcgac
    28681 ccttgtccgt gaaccgttcg aacatcccgc ctccttatgg tgatggggtc tgtaagagaa
    28741 gccttagctg agtgattcaa tgcaggtcaa aggctcgaaa atactgatga tgtgattcta
    28801 actagcgcaa tatggggtgt caaggtattg agcgaaacgc gagagccgcg cggcgactgt
    28861 tgaaacagcg gcgtgcgcgg cggaggaaat ctcattcttg ctgcgcgctc gtttgacagt
    28921 aaaagaatca tctcgataca atcccgcacc tttcaattgg cttctcatct cctcgcgacg
    28981 cggtctcatg tatgaaaagt aagagcatcg gcatcctgac gaaaccaaaa tttccggaag
    29041 tgaaaagcac ggtgcaagcc gtggtctcgt ggcttcgttc ccggaacatc gacgttctcc
    29101 tggatacgac cgcgacgacg ctgcttggcg agcaaggcgg gtttcaaaaa acgcatctcg
    29161 ccagcaaggc cgatgtgttg ctcgtgttgg gaggcgacgg cacgatgctc aatgcggcgc
    29221 gattggccgg cgagcgcggc attccgattc tcggtgtgaa tatgggcggt ctgggattct
    29281 tgaccgaagt cgtcttggac aatttgtatc cgtcgctcga gcggatgttc gcgaacgatt
    29341 tcgtgctcga cgagcgactg atgctgaaga cgcatgttca tcggcacggc gaaacggtcg
    29401 cccgcggcgt ggtcttaaac gatgtcgtca tcagtaaggg cacgctcgcc cggatgattg
    29461 aactcaagat cgcaattcag gggcagtttg tgacgaacct gcgcggggac ggactgatca
    29521 tcagcacccc gaccggatcg actgcgtact cgctttcagc cggcggtcct atcatcaatc
    29581 cggcggtccc gtcattgatt ctgacgccga tctgcccgca cacgctgaca catcgaccgc
    29641 tgatcgtgcc tgccagcgca gaaattgaag tggtcctgac cagtaaagat gatggggcga
    29701 tggcgacgct cgacggccag gtcggggtgg cattgaccca gggcgacacg gtggagatcc
    29761 gggcctccga gcacatgacc agactgatcc gtttccctga gagtagctat tatgaggtgt
    29821 tgcgagagaa gctgaaatgg ggcgacgggt aagcgaggct gagtggggcg cggctagccg
    29881 cgcccccgtt cgatgtgggc cagcatgtcg gcgttcgatg caaacttaaa ctctccgatg
    29941 gcctcgggag aggccgcatg agctttttcc accgtatcca acactttgag tccttcgaga
    30001 tccaccggcc gactctttcg atccttgagg atggtgtgaa gtttcgcgat cactccatcc
    30061 atcgttcctc gtgagcgcgt cgcttgagtc gtcaagtagc gtgagatcac ttccgcgcac
    30121 cagtccccgt cccgcttggc gatccccacc agaccttcgc tggctttgcg ggcccaaccg
    30181 gcgagaaaaa cgccatcgac cacctgccct gatttctcgt cataggcttg gaagagggaa
    30241 tcgtccggat cgtttccggt cttgttcggg ttggtgacgt agacgccgtt cttatagggg
    30301 agccccacgg tttcatcaac ccggtcgccc acggcaaaca cgacgctatc gacggggaac
    30361 tcatacaatt gtttgagtcc gaccgcggcg gtgtcttcac ctttcggctc cagcttattt
    30421 tcttccatct caagtgcgcg gacgcgattg ttggcgtcga ccagtacgcg cttcggggag
    30481 gccagaaaac gaaagcccat cttggagggg ctgcctgtgg gttcacactt cgtaaattcg
    30541 gcggtcatac tcgccagcac ttcgtcggca ttctgaccga ctgcagccag ccggtccttg
    30601 atgcgggcaa attccgtggc gatgccttcc tgatccatgt tggagcagac ggcccggatt
    30661 tctttcgggt tgtatttgcg ctcaaccggc ccgcggcggg cgatggcggt gacacgctcc
    30721 accttcttat acttgatcaa ccagtgggcg atgtcgacca tcacgtcgcc gacgccgatg
    30781 atcgcgacat gttttcccat gtcgaacgga cggtcgccga aacccggcag gcgattgaag
    30841 tggtacacta cgtccttggc atggaacacg cccttggcgg aatccccttc aaccccgatg
    30901 gcttttgtcc cttgcgcgcc gatggagaag gcgacggcgc tggcacccaa tccgcgcaaa
    30961 tcttcaaccg tgaggtcctt accctgcccg actgagacgt tgccgaagta gtggacattg
    31021 ggctgctcga gcatgtccca atattgcttc ttcaacccac cacgcagttt gagcttgctg
    31081 gggaagattc cgtactccgc caacccgccg aacttaatgt cgcgattgag aatgatgacc
    31141 tcgtggcccg catccgccat cttcttggcc agggccattc cagccgggcc tgcgcccacg
    31201 acgatgacga catgtccgct tgtctcagaa ccggttgcgc tcattatcta cccttcaatc
    31261 tatatcgaaa gtgaatcatt aatgacgaag accgtcgaac ggtcgaaagt ttaaaaattt
    31321 tgcgcacgtt agcacagggt tcctcgggct gtcaaaatcg ccgaccgccc cggtgaaccc
    31381 cggtgtggtc ggattatcac gcccggtgag ccggtctggc ggtcgtctac ccgcctgcac
    31441 tgccgaccgg tccgccggcc tgcgtcatac gccacggatc caggagttgc gtgagtcgct
    31501 tttccggcaa cacgttttgt tcgcgagcca cttcccggac ggtcttgccg gacttatagg
    31561 catccttggc gagttttgcg gccgcctcgt agccgatcac cggcgcgagc gcggtgcaca
    31621 tggcgagact ttcttcgatg agactcttgc agcgttcctc gttggccttg atcccatcaa
    31681 tgcatttcac tgcaaagttc gtggaggccg tcgccagcag ctcgatggac tgcagcaggt
    31741 tgtaggccat caccggcagc atgacgatta attcgaaatt ggccgcctgg ccgccgacgg
    31801 tgatcgtgac gtcattgccg atcacctgag cgcagaccat ggtgacggat tccgcaatga
    31861 ccggatttac tttgcccggc atgatggatg agcccggctg ggtttccgga agattgatct
    31921 cgccgagtcc gcagcgtggg cctgacccga gccagcgaat gtcattggcg atcttcatga
    31981 ggctgaccgc cagggtgcgc aagtccccgc tggcttcgac caacgaatcc tgggcggact
    32041 gtccttcgaa gtgattgacg gcttccttaa aagcgcatcc ggtttccttc gaaatgatgg
    32101 ccatgacctt gccggcgaat ttcggatggc aattgagtcc ggtgccgacg gcggtccctc
    32161 ctagagccac ttcgctgagc gccgcctgcg ctcgcttcat ccgtgcgatg cccagttcaa
    32221 tttgtctggc gtagccgccg aattcctgtc ccaaccggac cggcgtcgcg tcctgcaaat
    32281 gggtgcggcc gatcttgacg atcttgtcga actcgcgagc tttgccgttg agcgccttgt
    32341 ggagttgcgt aagtgcggga atcagttggc gttgaatcgt ttccgaggcc gcaatgtgga
    32401 tggcggtcgg aaccacgtcg ttactggatt ggcccaggtt cacatggtcg ttcgggtgca
    32461 ccagtttgct gcccctggcg ccgccgagca gttccgtggc ccggttcgaa atgacttcgt
    32521 tggtattcat gttggtcgag gtgccggagc cggtttggaa gatgtcgacc gggaattcgg
    32581 catccagacg tccctccacc acttcggtgg cggcgcgaac gatggcgtcc gccggcttct
    32641 tgtcgagtag tttcaacgaa tggtttaccg aggcggcggc gcgtttgatg agtcccatgg
    32701 cccggatcat cgcgcgggga atacgaagcg aactgatcgg gaaattttca atcgcgcggg
    32761 cggtctggac cccataatag gcattggagg gaaccggcag ttctcccatc gtgtcgcgct
    32821 ccatgcgcgt ggaagtggtc atggatgcgg cggtgccggt tgtgcgcgaa gctttcttca
    32881 tcgtcgggtc tcctcgaata gctggcggtt gacgtgctgg agccgcgcat cttaaacggt
    32941 ccccgtgcgg aagcacaagc atggctgtgt ccgtccgtga actaaacgga cggcgtcgac
    33001 ggtgagatgg taatgcgatg gcaggcagga gggtgaaaaa gaaaaggccc gccgggggcg
    33061 ggccttcctt ctcatggcac ccgatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33181 nnnnnnggtg aaaaagaaaa ggcccgccgg gggcgggcct tccttctcat ggcacccgat
    33241 ctcgttagga gatcttgacg tcgatctgtt tcggtttcgc cttttccgat ttcgggagat
    33301 gcaggtgcag aacaccatcc gcatactctg ccctcacctt gctctcgtcg accgaatcgg
    33361 gaagtgtgaa gctccggaca aaccgaccgt acgaccgttc gatacgatgg tactttttgc
    33421 ccttttcttc cttttcctgt ttccgctcac cctgaatggt gaggaccgca tcttcgaccg
    33481 tgaccttcac atcttctttc ttgacctcgg gaagctcagc cttaatggca tactcgctct
    33541 cggtttcgct gatgtcgacg gtcggagtcc agtcggcaac ggtcatgact tccttcccct
    33601 gtccggtagc cgtgccggtt gagggtcgcg caatcatccg gttcaaccgg tcggacatct
    33661 cctccaattc cctgaacgga tcccatcgta caatcgccat ggtggaacct ccttgtgtgg
    33721 atgtgttgac gcggttcact tggaacatcc tacgtactag aaaggaggta atcttcacga
    33781 agccgaagtc aagccctggg ccggaagagt taaagtcggt gacggggtct tgacattgag
    33841 acctccggca ttatttagta gccctcttca cacagggagg cgatgaccat gctggaatac
    33901 atgtcttacg cgctgctcgg cggtatcatg tttgtgacat cgattgtctt gtggtggttc
    33961 atcggtcgca cggagtaacc gtcactccgc taccgcttgt cgatcggcac gtagtgccgg
    34021 tcatgttcgc cggagtagat ctggtccggc cgatagagtt tgttctcccg cagttgctcc
    34081 agccaatgcg ccagccaccc gcttactcgc gccatggcga acagcggcgt aaagagatcc
    34141 acctcgatgc ccatcttgtc gtagatgata ccggagtaaa agtcgacatt cgggtatatc
    34201 cccttatccg ttaatcgttc acccgcgagt tgctccaccg ccaccgccac ttcatacagc
    34261 ggggacgttc cgcagacgtt gaagagacgc atgcagaggt cctgcagcac ggtcgcgcgc
    34321 ggatccttca ccttgtagac ccggtgcccg aaccccatga gtttgtgttt cccctccagc
    34381 ctggcttcga ctgccgcgcg ggcttgagcc ggggtgccga tttccctcag catcatcacg
    34441 acctcttcgt tcgcgccgcc gtgcaacggg cccttcag
//
LOCUS       DAOQRM010000011        31497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_823_length_31497_cov_16.826506, whole genome shotgun sequence.
ACCESSION   DAOQRM010000011 DAOQRM010000000
VERSION     DAOQRM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 31497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31497
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_823_length_31497_cov_16.826506"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKK81_02130"
     CDS             <1..300
                     /locus_tag="PKK81_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31695.1"
                     /translation="VPLRLDQFGNGSYDFKDGRFRTDVLSDHRWAGAWAQRENDREGG
                     FEITLSPDYSEGEGRWWYTRIESDAAPTKAGGTFHVMKVQSIVENQPVPGARASQ"
     gene            complement(310..1194)
                     /locus_tag="PKK81_02135"
     CDS             complement(310..1194)
                     /locus_tag="PKK81_02135"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV31696.1"
                     /translation="MEVFHMNVALLGTGLLGQAVAARLHATGHRISAYNRSPEKTLAL
                     RQQGIRIASTAAEAIAAAEVVILLLADAAAIRAVLLDPSTSQALGGRTIIQMGTIGPS
                     ESCSIGEEIERLGGQYLEAPVLGSIAEAKSGALLIMVGASPERYAAWAPLLQALSSDV
                     RLIGPVGKAAVMKLALNQLIAAETAAFALSLGLIRRGGVDVENFMAVLRKSALYAPTF
                     DKKLPRLAERNYARPNFSTRHLLKDVELTLAAADSAQLSPGGLPGVRSLLSDTIALGL
                     GDVDYSALYERVDPVTGT"
     gene            complement(1245..2057)
                     /locus_tag="PKK81_02140"
     CDS             complement(1245..2057)
                     /locus_tag="PKK81_02140"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNV31697.1"
                     /translation="MDHELSILSEAMRRAGREALRMAATGFDTYTKPDQSPVTSADLA
                     VNQILRDHLSAAFPEDGWLSEETEDNHNRLTRSRVWIVDPIDGTRSFVRGLPEFCLSV
                     ALVEQGMPAVAAIFNPATGEFFSAIRGQGIRVERQAEAGQAPFTPPERPLALVNPWEL
                     RVGRFQALESHLHCRPIGSIAYALALVAAGQADAVVTLEGGNEWDIAAGVLLIEESGG
                     RATTAAGHPFTFNRADPRVKGTLAIGTDLTTPTRTHLIQLSAASRDLRAPLP"
     gene            2207..3265
                     /gene="corA"
                     /locus_tag="PKK81_02145"
     CDS             2207..3265
                     /gene="corA"
                     /locus_tag="PKK81_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNV31698.1"
                     /translation="MKLKHKHSRKAGLPPGTLVHIGEKKSETVTISVYEYGEGQFQER
                     AVSKPDEIAMTGEPTVRWVDVGGIHKMEVLESFGKMFGLHPLLLEDIANTDQRPKLDD
                     YGSYGYVVLKMLYEGDREGDINVEQVSLVFGENFLLSFQENGGDVFQGVKERLRNGKG
                     RLRHAGADYLLYALMDSIVDRYFVILETLGERIEALQDLCVTNPGPETLRDIHGLKRQ
                     LLFLRRSVWPLRDVMNNLSRSDGQFLQGSTKVFFRDVYDHIIQIGDTIETLREMVSSM
                     MEVYLSSVSYRLNGVMKVLTIITTIFMPLSFIASIYGMNFEHMPELKSPWGYPAVLGV
                     MAATGLAMLYFFKQKKWL"
     gene            complement(3286..3675)
                     /locus_tag="PKK81_02150"
     CDS             complement(3286..3675)
                     /locus_tag="PKK81_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31699.1"
                     /translation="MTWWETILFPVAWLWRPKLLFALLFFGGAAYLLIIFNWSYSDGD
                     RVGYLQKLSRKGWICKTQEGELAMTTVPGVAPVLWSFSVWDDAVAKKLDGQMGKRVVL
                     HYKEFRYIPTTCFGETAYFVDRAEVLD"
     gene            complement(3697..>4318)
                     /gene="leuD"
                     /locus_tag="PKK81_02155"
     CDS             complement(3697..>4318)
                     /gene="leuD"
                     /locus_tag="PKK81_02155"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNV31700.1"
                     /translation="MDAFTTLTGLVAPLDRLNVDTDQIIPKQFLKTIKRTGLREGLFY
                     DWRRLKDGSQDPAFFLNQPRYHNATILLARDNFGCGSSREHAPWALLDQGFRCVLAPS
                     FADIFYNNCFQNGILPVVLTGPEIQALFEGVTAREGYQLTVDLAAQQVTTPNGATYHF
                     EIDPFRKDCLYRGLDAIGLTLQHAGRITEYEQRRRTQAPWLFQDVR"
     assembly_gap    4319..4418
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4419..5548)
                     /gene="leuC"
                     /locus_tag="PKK81_02160"
     CDS             complement(<4419..5548)
                     /gene="leuC"
                     /locus_tag="PKK81_02160"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNV31701.1"
                     /translation="MAAQTLFEKIWNDHVVRAEPDGTTLLYIDRHLVHEVTSPQAFEG
                     LAVAGRTPRRPGAALAVPDHNVPTTDRRVAIADPISAKQIQTLDDNCNTFGITMFGMN
                     DIRQGVVHVIGPEQGFTLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLV
                     QKRPKTMEIRVDGQLSPRCSAKDVILAIIGKIGTAGGTGYVIEYTGSAIRALSMEGRM
                     TLCNMSIEGGARAGMVAPDDTTFAYIKGRPMAPKGALWDQAVTAWRQLATDPGAKYDA
                     VVELRAETIAPQVTWGTSPGMVTGVDGNVPDPRTMGDDKLRQATEQALEYMALLPGMP
                     IRDIKIDKVFIGSCTNSRIEDLRLAASFAKGKKVASTVHAMVV"
     gene            5709..5942
                     /locus_tag="PKK81_02165"
     CDS             5709..5942
                     /locus_tag="PKK81_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31702.1"
                     /translation="MEQEPVDAERITIPRWTIKRDPRALGCTICGSAIVKITAEWRQV
                     SFCTCRLIEYRVLPRAPKALPAPSTAADVRKEP"
     gene            6043..6594
                     /locus_tag="PKK81_02170"
     CDS             6043..6594
                     /locus_tag="PKK81_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31703.1"
                     /translation="MARWLGAFVVAGCLGVAGWCPAEAKDHPAAKSLMRTGLATHGHS
                     SRTCAGDDLPGNWHLVAFDSSYRFRNPQAPYLFPHQAFQYSHRGGAKSAHSLQPLSGD
                     PDQLFEAVPLDMTYRVEGKGRVVLKTRGHTETVETWTCRVITQDPEAMGDGTRLQRGD
                     LVMTLIGSNGQGLFVRHLRKRAA"
     gene            6686..7159
                     /locus_tag="PKK81_02175"
     CDS             6686..7159
                     /locus_tag="PKK81_02175"
                     /inference="COORDINATES: protein motif:HMM:NF020254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HNV31704.1"
                     /translation="MDDPNQVWSQPSVVEWSQLLLNSYRHWVGRDLMLRTGGPEEQAH
                     ALFMAPFVVVSHGAQEDPILNYGSHLALTLWETTWEQFLQTPSRLTAEAVNRAEREWM
                     LERARVQGYVDNYRGVRISNSGRRFLVESAIVWSVIDQAGRRQGQAATFSRWTFL"
     gene            complement(7139..7357)
                     /locus_tag="PKK81_02180"
     CDS             complement(7139..7357)
                     /locus_tag="PKK81_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HNV31705.1"
                     /translation="MMKPGRYRHYKGNDYEVLGVARHSETEEEYVVYRQLYGAGGLWI
                     RPLGMFLESVTIGETVVPRFRRLEEGPA"
     gene            7407..8816
                     /locus_tag="PKK81_02185"
     CDS             7407..8816
                     /locus_tag="PKK81_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV31706.1"
                     /translation="MSSQLSYMPERRHDVVVIGGGSAGYAAARTARDAGADVAIVDQG
                     PLGGLCILRGCMPTKTILRSAEIIALMKRAREFGLSPVTAQADLSAIVDRKNRLVQEF
                     ADYRIAALRDPRFTLYESRAEFLSPHELRAGDVRIRGGAFVIATGSSAAEVAIPGLDE
                     AGYLTSDEMLDIRRQPASLLVLGGGLVAVEFAQFFARIGTKVTMLQRSTTLLSDMDED
                     ISQALAAAFQDEGIEVMTGVSFQRVSSTPSEKTVHFVQGGAVHTRSAEVIFQALGRRP
                     NLKGLNLKAAGVCVVDGRLVVGGDMRTSQPHIFAVGDVNDLTPIVHLAIEQGETAGFN
                     ATHPNEPARVIDHRLDAEVVFTEPQVAVAGMSERQCREEAIPYLTASYPFADHGKAMC
                     LGAVHGFVKLLCRPDDGALLGAQIVGPEAGELIHELIAVMYFRGTAQDLLRIPHYHPT
                     LAEIVTYPAESIVEQMGRA"
     gene            8813..9496
                     /locus_tag="PKK81_02190"
     CDS             8813..9496
                     /locus_tag="PKK81_02190"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV31707.1"
                     /translation="MKRTVAAGASALLLLVTLACGQQTWDAAMQAGETALQRGQYEQA
                     EKIFSAAVHKAEEFGLHDRRVAVTLAHLAQAYSAQGKFVEAEPVYLEALKIYQDVHGE
                     NHLDVAAMLNNLGVLHRKHGQYADAQRLLTRALSIKEKLLGTDHPEVALALSNLAAMY
                     LAQGDGEQAGALFARALAVREKHLGPDHPDVAKNLEDYASALRKLGRVGEAEGLERRA
                     GAIRAKPKS"
     gene            9573..9788
                     /locus_tag="PKK81_02195"
     CDS             9573..9788
                     /locus_tag="PKK81_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31708.1"
                     /translation="MSDLRTNALIARGMGIVFIAGGFMLGIYGLEFPNTMWLQTALGL
                     LVTGMMAQGYAMYCSIRRVREQRDSER"
     gene            9817..10263
                     /locus_tag="PKK81_02200"
     CDS             9817..10263
                     /locus_tag="PKK81_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31709.1"
                     /translation="MVCPGRWSALLLLLCVAMLSGCGGPWRDRYLDKGVKKLTQDDIA
                     EKFGPPSTAKTPVLGGDTVWTYRVPMGDREVHPWDPGSLTAGKKTKAPPSPSPFGKSL
                     AGNDEPYREPLYCYRYTLSFDEAKLLKDWKREECVPRTSEDETASQ"
     gene            10281..11105
                     /locus_tag="PKK81_02205"
     CDS             10281..11105
                     /locus_tag="PKK81_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNV31710.1"
                     /translation="MRELLPVIGSSVFFDLLKSILLLLILLIARTVLVRWIKGNHTLT
                     IDAKRRWIVTARNSMVLGLFVGLVVIWAHELEAFAVSLVALAATVVLATKELILCWSG
                     AALRVGGGVYGVGDRIQLGTYRGVVLEYDVFATKLLEIGPGQTSHLYTGRTVVFPNSL
                     LFGNPLIKESPSQEYGLYVLSVPLQSTDDWQAAEQALLNAAKVECASFMDQMGRQMKL
                     LEQRNLLEAPSPEPRITIQLPEAGRIHLVLRFPAPDRGRSRVEQAILRRYLIAVRP"
     gene            11306..11710
                     /locus_tag="PKK81_02210"
     CDS             11306..11710
                     /locus_tag="PKK81_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31711.1"
                     /translation="MRRSIAVLVAVAFVGMSSFAVADDMGLGKMSEGMKQDMSGLSND
                     LNAQKEDLASDLKAKKESVKSEMKAKRDEAKAKKKQAKADLKAKKEHMKAKVKAKKDA
                     ARAKAAEARSVVDDAAHEAGGMKNDVTGAMRN"
     gene            11852..12625
                     /gene="xth"
                     /locus_tag="PKK81_02215"
     CDS             11852..12625
                     /gene="xth"
                     /locus_tag="PKK81_02215"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNV31712.1"
                     /translation="MKVATFNVNSLRKRLPIVLQWLERHQPDVLCLQETKVQDSEFPL
                     TALATSGYEITFRGMKAYNGVAILSRTKPEAISYGFDDGGETEDARLLRVVIQGIPII
                     NTYVPQGFEIDSPKYQYKLGWYDRLRKHFETHLSPKEPAIWCGDMNVAPRPIDVHSPE
                     KHLKHVCYHEDARNAYGKTVAWGFEDVFCRLYPDRQQFTFFDYRAPSALAANKGWRID
                     HILATPPLAQRCQLVAVDLEPRRATDPSDHTVLWAEFST"
     gene            complement(13628..14038)
                     /locus_tag="PKK81_02220"
     CDS             complement(13628..14038)
                     /locus_tag="PKK81_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31713.1"
                     /translation="MHKRKKSLVFLRRPKKFERNTAGTHRPDHRSHFERRLVVLKRDL
                     KIKDIVHMHRSLALNDAPAHRNVQDGSFAPDFAAREGQKQAHGNTQMFSPIEHLRRIT
                     ASQSEGLETEATRLTMERGNTSKRLQPLHQRGAA"
     assembly_gap    14141..14240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14256..14561)
                     /locus_tag="PKK81_02225"
     CDS             complement(14256..14561)
                     /locus_tag="PKK81_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31714.1"
                     /translation="MALDRSTDTPGGFQVRHRALGVFQGSSIGLAFWHPSSHMPEYGL
                     CRFATEAKAQDYVDFLSSPACPEPLNRADLIVEPFDHIEHDRLTTEYPQASAWETPL"
     gene            14723..15325
                     /locus_tag="PKK81_02230"
     CDS             14723..15325
                     /locus_tag="PKK81_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNV31715.1"
                     /translation="MFMSLTRSMGGVVLLAAVLALPGCGYNDLQGLDEDTKAAWSEVI
                     NQYQRRADLIPNLVNTVKGYAAHEKETLESVVQARAKATSVQVTPELLKDEAAFKKFQ
                     EAQQGLSSALGRLLALAENYPNLKADQGFRDLQSQLEGTENRITVARKRYIDKVAEFN
                     KMVRYFPTNLTAKFLLHLEEKANFTVADEKAVAKPPEVKF"
     gene            15336..16262
                     /locus_tag="PKK81_02235"
     CDS             15336..16262
                     /locus_tag="PKK81_02235"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNV31716.1"
                     /translation="MSQGAIAMRLMGRTGWGLLIGWLWLTGVASLPAAALDVPLLSGR
                     IVDQAHALPPAVAERLSADLAAHEANTSNQVAVLIIPSLEGEPLFDFSHRVATAWKLG
                     RKGTDNGVLLLVSIKDRKIRIEVGYGLEGVLTDARSAQIIRNEIVPRFRAGDVPAGVT
                     AGVQAILQTIEGTYRASEHPTATPSSSDAFATAAFAVILGVVVGLFFSRAHRVLGPVV
                     GGGLSFLAAPWVIPAVMAGAVSLVLVGLLSKLGPSSGSRRRGDGSAFDGTWFSTQQGG
                     WGSGGFGGSFGGSGGFSGGGGDFGGGGASGDW"
     gene            16249..16911
                     /locus_tag="PKK81_02240"
     CDS             16249..16911
                     /locus_tag="PKK81_02240"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNV31717.1"
                     /translation="MATGRRPTLTDEERARVEAAVQAAEQRTSAEIVPMIVGRSGLYR
                     EAHHRAGLLSAVLALTALLTIETTWLPWGWHAANAVWLLGVIMLAYALGSWVGTWPPV
                     LRLFTSRERMHRKVQLRAKLAFAQHGLAQTRERTGLLLLISLLERQVSVSADQSLRDG
                     ISDAQWQEVVAVVVERLKVGDLVGGLCCGIEASGTLLARACPARDGDNPDELSNQVIQ
                     DT"
     gene            16970..18598
                     /gene="murJ"
                     /locus_tag="PKK81_02245"
     CDS             16970..18598
                     /gene="murJ"
                     /locus_tag="PKK81_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNV31718.1"
                     /translation="MSDPAASVEPSRPAPDETRSVVKAAGLIGVATFSSRILGFVRDM
                     VLARLFGATPAADAFFVAYRIPNLLRELFAEGSMSAAFIPVFTEYHTLKTKRDAWELA
                     SATFTTLLTIVTAVTLLGILAAPGIVWLLAPGFRGSPDKLALTTLLTQMMFPYLIFIS
                     LAALAMGILNSLRAFAAPAFSPVFFNIFTIACMMFLSPWLPEPIIGVAIGIVVGGVAQ
                     FAMQLPGLKGRGMLFGLRFQPGHPGVKRIGWLMVPSLLGLSVTQINITVSTILASYFA
                     GGPTYLFYGMRLIQFPLGIFGVALATAILPTLSAQAARGALDELRTTIGFGLRMIFFI
                     ILPAMLGLILLRHPIVHLVFEHGSFTKADTLATATALLCYAVGLWAFAGVRIIVSAFY
                     SLQDTRTPAVTAGIAVGANILLSLWLMTLLDAAGLALATALASMLNGSILVAVLHRRL
                     GRVDWGSIVWSAFRVLGACIPVAVMCLWVANASLWDQDGAWVVKSAALFGGIGVSVIG
                     YLGVHIMLGSEELDVVLGMVKRKLGRVAKKFGAA"
     gene            18600..19103
                     /locus_tag="PKK81_02250"
     CDS             18600..19103
                     /locus_tag="PKK81_02250"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNV31719.1"
                     /translation="MTHAITFQTAWGLIRITASEKGVTSIDLSPSDPAARQQPAEAGG
                     AAASIVEEARAQLVAYIAGTRREFSFSVDYSAGTPFQRKVWKAITRIPYGRVRSYQWV
                     AMRVGGKQYARAVGMALGANPVPIVVPCHRIISHDGSLGGFSCGLPLKRRLLSLEGTL
                     NQLRNAQ"
     gene            19138..19608
                     /gene="dtd"
                     /locus_tag="PKK81_02255"
     CDS             19138..19608
                     /gene="dtd"
                     /locus_tag="PKK81_02255"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNV31720.1"
                     /translation="MKVVIQRVTRASVEVEGRIIGRIGAGLLVLLGVAKGDDERDLLY
                     IVEKLQTLRIFGDEQGKMNRTLIEAGGALLLVSQFTLLGDTSKGRRPGFDLAALPEEA
                     RTLYEQAVGRLRAKGLTVETGLFGAHMQVELLNDGPVTFLLDSRRGASAPTLKS"
     gene            19707..20273
                     /locus_tag="PKK81_02260"
     CDS             19707..20273
                     /locus_tag="PKK81_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31721.1"
                     /translation="MGSPVSRSHPLRQLFGALTEKSFTEHLGWPDLNVTEYVSNLLVE
                     FAHTDQLYKIQNTQGRAVDSVVEMLFESEVLLEAQSFEREREVHRHIGDFTLFMTGLF
                     PEYLRRLKSVGRIYHKDFLVDYVKTGKRSYGLVAEYGSYDPEQDSPLFRKLSENFELC
                     VTGLGFVRSDLDRMQDPACRRVKDLLLN"
     gene            20183..21208
                     /locus_tag="PKK81_02265"
     CDS             20183..21208
                     /locus_tag="PKK81_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HNV31722.1"
                     /translation="MCDRFGVRPVGPRSYARSCLPPRERSPAELKPSRPILLIHGGAG
                     RRAMTVAQAACVETALAEGHRLLMAGTPALSVVEEAIRLLEESGLFNAGRGSNRQLDG
                     VRRMDASMMEGRDLRAGAVASIEGIVHPITAARLVMEKTAHVLLVGQSASRFADYFGL
                     ERAPRAKRTAGPQPKDLRPRAGLKHTLRLHQAIMQTGRLRARSLGKETVGAVALDLDG
                     TVAAGASTGGVDVMLPGRVGDTPLIGCGVYADNEAGAVSMTGLGESIIRVAVAKEISD
                     LLASGLSPLRSANRVLRKVVERIHGTAGVLVLSPDGRFAIRHSTPNMVAGVIGRDGRP
                     HVKGRFA"
     gene            21249..21677
                     /locus_tag="PKK81_02270"
     CDS             21249..21677
                     /locus_tag="PKK81_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV31723.1"
                     /translation="MPALFHLAFPVHDLAAAKQFYVEGLGCVLGRESSSAVTFGLAGH
                     QLVAHLSTQSTVPQKGVYPRHFGLVLTQEEEWQALADRAKAKGLTFYQQPRRRFAGTR
                     IEHLTFFLQDPSRNLLEFKYYRHESAIFGERDVAAVGDAE"
     gene            complement(21726..22511)
                     /gene="mtgA"
                     /locus_tag="PKK81_02275"
     CDS             complement(21726..22511)
                     /gene="mtgA"
                     /locus_tag="PKK81_02275"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNV31724.1"
                     /translation="MSKSTGSLFGRMLLWATILVGLPAAALALYWLVTLPDVSHLAKH
                     HPTETAVMEARRAQAKEQGHPLHIKFSWVPLARIAPALQRAVVAAEDASFFAHEGFDW
                     EGIKDAALYNLEVGEFKRGGSTITQQLAKNLYLSSERSLLRKAREALITRSLEHHLTK
                     ERILELYLNVAEWGQGVFGAEAAARHHFGKSAKELTIDEAALLAAILPSPRRYDPIRH
                     TAYLNRRQHHIVRWLERGNGRRAGSLSTNRQAPEPHDLAPSEN"
     gene            22613..>23208
                     /gene="yihA"
                     /locus_tag="PKK81_02280"
     CDS             22613..>23208
                     /gene="yihA"
                     /locus_tag="PKK81_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNV31725.1"
                     /translation="MKLLSAEFIKSSVSPEQFPPDRLPEIAFVGRSNVGKSSLINSLL
                     HRKKLAKVSKTPGKTRAVNFFTVRTSDPKLSLLSLVDLPGYGYAKVSKTMRAQWGPMI
                     EQYLEQRQSLGAVILLIEARVWMPQDVMTVQWVQSLGCGMIIVLTKADKLRQSERAPS
                     LVAVRTACGLGPEVPIVLYSAETHEGRDALWGEIRTQF"
     assembly_gap    23209..23308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23309..24343)
                     /locus_tag="PKK81_02285"
     CDS             complement(23309..24343)
                     /locus_tag="PKK81_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV31726.1"
                     /translation="MNDQSLSAHTCLHGYRFFLSQKGRPAPGLVRSTTGLTTTGALFF
                     CLCLLFVLIANAEAAKLEDRIVAVVNSDLIMLSELKRDLLPEQDRLRKLYKGEDLERR
                     LKTAEAMGVTKMIERKLQLQAARNKGVDVSDQEVVQAVEEMKKQGEAIDSADPNTARS
                     IREQLTLMRVVDREVRGLIMVADSEMKRYYQEHQDRFAYPEEYQLSQILIKPRTPDGL
                     SAAHGRAEALLATLKQGEPFEDLALRFSDGADASRGGRLGLVRQGELIPALEQALTSV
                     DVGQITGIVETAEGLHIVRVDDKKPRQFRPFEQVKAEIQSLVFQQKTEDQYQIWMADL
                     KNKAYIEIKF"
     gene            complement(24453..25349)
                     /locus_tag="PKK81_02290"
     CDS             complement(24453..25349)
                     /locus_tag="PKK81_02290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV31727.1"
                     /translation="MTPPRTRQISSWQTRLTSAALGLCALGSLAACTEPPQEEPVIAM
                     INGRSITQSEFDIRWEDLSQATRARYEKEGGKRRFLDELIMRELLMQEARKQGLDQSD
                     EIREKTLRYREQLILDELLKDRIKTKVEVSKEELDAYLGKHANQLLANPKVQVSIMLL
                     PNVYAAKDLKRQVEAGGNFTRFALRYSIDERSRAKGGDLGPYKKGLLEPELDALIPSL
                     HPGVISDPIKTAQGYYLVKVSPLEPEILQADQATRERLRQELLAEKRRKRLDDVFAEL
                     RTGATIRLADAARYVTDEPARP"
     gene            complement(25380..>28718)
                     /gene="mfd"
                     /locus_tag="PKK81_02295"
     CDS             complement(25380..>28718)
                     /gene="mfd"
                     /locus_tag="PKK81_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNV31728.1"
                     /translation="HSLPSQPLADRSWLIVAKNDDEAERLYRDTLFFRTLCGQSGDDL
                     ALFPKWETLPYESTVPHIDLVARRMQTLNRLCTTARTVLFTSIPALTQRVLPALVFTD
                     AILQFQPNGSLEREVLVSSLLRLGYRKGSVVEIPGEFSIRGGIVDIYSTAYADPLRVE
                     FLGDTIESIRFFDPATQKSTDKIKQAWVLPARELIRPEDAPDALAPLAADAEWHAPSV
                     YGTMDSLLDYFPQPPVLVLDQPGTLKAHTEECWQAIEEGFLRHEDRTDPNPYPTPDNL
                     YLTWDQILAATQGYATLALEPVTAPDASWDPVLTCPAQTPASVGLGQRGTAFSHTLEM
                     LDRLREGGPVVLVARSQGQVGRLLALFGEHDRPAVEWKPSALSAGGAQKAPFSVLNGE
                     VSAGFLSPDLRLVVLTEEELFAKGARHKPQPKSKAATFLSSLEDLNIGDYVVHMQYGI
                     AKYQGLRRLSVQDFDSDFLVLEFAGTDKLYVPLDRLSQVQRYAGADAHVPRLDRLGGT
                     SWAKTTARVKKDIEEMAHELVDLYANRELVHRTSYGKDSMLYHEFEAAFEYEETPDQR
                     KAIEDIANDLASTKPMDRLVCGDVGYGKTEVAMRAAFKAVEENRQVAVLVPTTLLAHQ
                     HYDNFAERFAPFPTRVALLSRFQSPKDTKAIVKDTEAGLVDVLIGTHRLLQKDVKFRN
                     LGLVIIDEEQWFGVKHKERLKQLRTQVDVLTLTATPIPRTLQMTMASVRDLSIIDTPP
                     SGRLAIRTQVLRFSEKAIREAILRELGRGGQTYFVHNRVETMERMGAWLQELVPEARI
                     VMAHGQMDSKPLEAVMLKFFHREADILIASAIIQSGIDVPTANTIIVNRADTFGLAQL
                     YQLRGRVGRGGEQAYAYFLVPDEGNLSEDAQKRLTAIQQFTELGSGFRIAAADLEIRG
                     AGNLLGKQQSGHIAAVGLDLYLQMVEQAVQRLKGQVVEEEPDPTLRLSVSAYIPEDYV
                     ADSHQRLSLYKRLSSCGQLGDLALMHGEIEDRYGHPPDPVERLFELMQIRLLAKQLRL
                     SSVVEQPHAVVITFDPKATVSETAVQALMDRYKKRLRFLSPLSFELQIPHEDWSLVFP
                     ELNATLQTLHVCGTNNQGPRTAST"
     assembly_gap    28719..28818
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28819..29297)
                     /gene="rfaE2"
                     /locus_tag="PKK81_02300"
     CDS             complement(<28819..29297)
                     /gene="rfaE2"
                     /locus_tag="PKK81_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNV31729.1"
                     /translation="MHTKVTTRQELASRLEERRRQGQRIVFTNGCFDLMHIGHIRYLQ
                     AARNLGDVLVVGVNSDESVRALNKGTDRPIVPDAQRAEVLAALACVDYVVTFSEPDPG
                     ALIATLQPDILVKGGDWSVDRIVGRETVEARGGRVQTIPLVPGVSTTTLVQRIRSTTA
                     "
     gene            complement(29388..30581)
                     /locus_tag="PKK81_02305"
     CDS             complement(29388..30581)
                     /locus_tag="PKK81_02305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNV31730.1"
                     /translation="MSGRWPRQILFGDIDAMFASAAVLADPSLAGKPVAVGGPPPRGI
                     VAAASYEVRRFGVRSAMPTIQAMRLCPQLVLVPPDRPLYQRLHRQLQDITDRFFPETE
                     WTSIDEFYADTTRLQTRHPDPRELGQALKIDITRVTGLTCTIALASGKTVAKIAADAH
                     KPDGLAVIEPGSEAAFLAPLPIRSLPGMGPKSTEAIERLGIHTVGDLLEPRFEQSLIR
                     LLGSRLAAFQSLARGHDSDPVVADREAKSMSHETTFDEDTNDPALLEPIIHGFLETLT
                     HDLRLEGLAAGSFTVKLKDSHFRITTRQRKFSSPVNYDPDMWPDIRLALAELLQPRSR
                     YRLVGLGLSDLVPAPEPLFDRRQRDAVAILDRLIQQHGAGVVRLGGLPQGGDSPKKRG
                     RRTEN"
     gene            complement(30589..31200)
                     /locus_tag="PKK81_02310"
     CDS             complement(30589..31200)
                     /locus_tag="PKK81_02310"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HNV31731.1"
                     /translation="MSPSPEPLLTIWTDLNRRYFQGTLPPIPIEWSRRLTSSAGMFVC
                     RIGPRQPLARTTEAPTRRRCIRLSAVLLKPNNPESSHETVMTLAHEMIHQWQYDILKR
                     RPNHGADFRRMMDQMNRDGLGITIYHSLGKEVAALAKYAWRCQHCGCLYQRQRRTIQP
                     RRHFCGACRGPLRELPAVVPASTAEPAHTSAAGPQLGFPFTTV"
     gene            complement(31258..>31497)
                     /locus_tag="PKK81_02315"
     CDS             complement(31258..>31497)
                     /locus_tag="PKK81_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31732.1"
                     /translation="SAVILPEVFLKAVSLGRNLGHPLTNITTVNMDFLTHYRPMTNVV
                     RRPTQKGGKGYALTGHHEIMVPLLAAAVIEELSGR"
BASE COUNT         5920 a         8645 c         9941 g         6591 t
ORIGIN      
        1 gtgccgttga gattggatca attcggcaac ggctcgtatg actttaagga tggacggttt
       61 cggacggatg tcctctccga tcaccgttgg gccggggcct gggctcaacg ggaaaacgat
      121 cgggaaggcg gattcgagat caccctgtcg ccagactact cggaaggcga aggtcgttgg
      181 tggtacacac gcatcgagag tgatgccgcg ccgaccaagg ccggtggaac gttccacgtg
      241 atgaaggtgc aatcgatcgt cgagaaccag cctgtacccg gcgcgcgcgc ctcccagtag
      301 agaccagagt cacgtgccgg tgacagggtc cacccgttca tagagcgctg agtagtccac
      361 atctcccagc cccagggcaa tcgtgtcgct gagcagtgat cggactcccg gaagtccgcc
      421 gggagagagc tgggcactgt ctgcggcggc gagggtcaac tccacatctt tcagcaggtg
      481 gcgggtcgaa aagttcggcc gggcatagtt gcgttccgcc agtcgaggca acttcttatc
      541 gaacgtcggt gcatacagcg cactcttgcg taacaccgcc ataaaattct ccacatcgac
      601 cccgccacgg cgaatgagtc ccagactgag ggcgaacgct gccgtttctg cggcaatgag
      661 ctgattcagc gccagcttca tcacggcggc ttttccgacc gggccgatca ggcgcacgtc
      721 cgaactgagg gcctgcagga gcggtgccca tgcggcataa cgctccggac tcgcaccgac
      781 catgatcagc agggcgcccg atttggcttc ggcgatactt cccagcaccg gcgcttccaa
      841 gtactgtccg ccgaggcgct cgatctcttc tccgatcgag caactttccg acgggccgat
      901 cgtgcccatt tgaatgatgg tgcgtccgcc gagcgcctgg ctggtggagg gatcgagcag
      961 cacggcacgg atggccgccg cgtctgccaa caggaggatg accacctctg cagcggcgat
     1021 ggcttctgcc gcagtcgagg caatgcgaat gccctgttgc cgcagcgcca gggtcttttc
     1081 aggagatcgg ttataggcgg atatgcggtg accggtagcg tgcagccgcg ctgccaccgc
     1141 ctgccccagc aaccctgtgc ctagcaaggc cacattcata tggaaaacct ccatcctgta
     1201 tgcgagacgg tcatcgtaca gagaagtggc cgcggttgaa agggtcaagg gagaggggcg
     1261 cgaaggtctc tacttgcggc actgagttga atgaggtgtg tgcgcgtcgg tgtggtcagg
     1321 tctgtgccga tggccaaggt gcccttcacc ctgggatcgg cgcggttgaa cgtaaacgga
     1381 tggcctgcgg ccgtggtggc gcgccctccg ctttcttcga tcaacaggac gccggcggcg
     1441 atgtcccact cattgcctcc ttccaacgtc acgaccgcat cggcctgccc ggctgcgacc
     1501 aaggcgaggg catacgcaat cgatccgatc ggacggcaat ggagatggga ctccagggcc
     1561 tgaaagcggc cgacccggag ttcccatgga ttcacgagcg cgagtgggcg ctccggcggg
     1621 gtgaacggag cctggccggc ttctgcctgg cgctcaacgc ggatgccctg gccgcgaatg
     1681 gccgagaaga attcaccggt cgcgggattg aagatggctg caacggccgg catgccctgc
     1741 tcaacgagcg ccactgagag gcagaattcc ggcaggcctc gcacgaatga gcgcgttcca
     1801 tcgatcggat cgacgatcca gacccgtgat ctggtcaggc gattgtggtt gtcctcggtt
     1861 tcctccgaca accagccatc ctccggaaat gccgccgaca aatggtcccg aaggatctga
     1921 ttgaccgcca ggtcggcaga ggtgacgggt gactgatccg gcttggtgta ggtgtcgaat
     1981 ccggtcgccg ccattcgaag ggcttcacgc ccggcccgcc tcatggcttc cgacaagatg
     2041 ctgagttcat gatccatcgt cacaggggct cccggttcgc ggagaagggt gatggctgca
     2101 tgtcttcacc gtaccaggtc ttggttcggg aacaaagccc ggagtggctt gatgccgcgc
     2161 ccggctcttg accctcctgc cggttccgcg tacaagctga ctgaccatga agttgaagca
     2221 caaacattcc cgcaaagcgg ggttgccgcc cggcacgctg gtccatatcg gcgaaaaaaa
     2281 gtccgagacg gtcacgatct cggtgtacga atacggcgag ggacagtttc aagagcgagc
     2341 ggtgtcgaaa ccggacgaga tcgcgatgac gggggagccg acggtccgat gggtggacgt
     2401 cggcggcatc cacaagatgg aggtgctgga gagtttcggg aaaatgttcg gcctccatcc
     2461 cttgctgctg gaagatatcg cgaataccga ccaacgtccg aaactcgacg actacggatc
     2521 gtacggctat gtggtgctca agatgctgta cgaaggggac cgggagggcg atatcaacgt
     2581 cgagcaggtc agcctggtat tcggtgagaa ctttctcctg tcgtttcaag agaacggcgg
     2641 ggatgtcttt caaggtgtga aggaacggct gcggaacggg aaaggccggt tacgtcatgc
     2701 cggcgcggat tatctcttgt acgccctgat ggactcgatt gtggaccggt atttcgtcat
     2761 tctggagacg ctgggagaaa gaattgaagc gctccaggat ctgtgcgtca cgaaccctgg
     2821 tccggagaca ttgcgcgata tccacggcct caagcggcaa ctgctgtttc tgcgtcgctc
     2881 cgtctggccg ctccgggacg tgatgaacaa cctgtcgcgc tcggacggtc aatttctgca
     2941 gggttcgacg aaggtgtttt tccgcgatgt ctatgaccac atcattcaga tcggcgatac
     3001 gatcgagacc ttgcgcgaaa tggtgtcgtc catgatggaa gtgtatctct ccagcgtgag
     3061 ctatcgcttg aacggcgtca tgaaagtgtt gacgatcatc acgactattt tcatgccgct
     3121 cagctttatc gccagcatct acgggatgaa tttcgagcac atgccggaat taaaatcgcc
     3181 ctggggatac ccggccgtgc tgggtgtcat ggccgcaacc gggctggcca tgctgtattt
     3241 tttcaagcaa aagaagtggc tgtgatggcg tgcagaatgg ggccgtcaat ccaagacttc
     3301 ggcgcgatcc acaaagtagg cggtttcccc gaagcaggtg gtggggatat agcggaattc
     3361 cttataatgg aggaccactc gttttcccat ctgcccgtcg agtttcttgg ccacggcatc
     3421 atcccacacg ctgaaactcc ataataccgg tgcgacgccg ggcacagtcg tcatggcgag
     3481 ttcgccttcc tgggtcttgc aaatccaccc tttccgagaa agcttctgga gatagcccac
     3541 gcggtctccg tcggaataac tccagttgaa gatgatcagc agataagcgg ccccgccgaa
     3601 aaagagcaac gcgaagagaa gtttcggtct ccacagccag gctaccggaa acagaatggt
     3661 ttcccaccac gtcatagggc gttcctttcc cgacggttag cgcacgtctt gaaacaacca
     3721 gggggcttgc gtgcggcggc gttgttcgta ttccgtgatc cgaccggcat gttggagcgt
     3781 gaggccgatc gcgtccaatc ctcgatacag acaatctttt cgaaacgggt cgatctcgaa
     3841 atggtaggtc gcgccgttcg gcgtggtgac ttgctgtgcc gccagatcca ccgtcagctg
     3901 atagccttcg cgggccgtca cgccctcgaa gagcgcctgg atttctggcc ctgtcagtac
     3961 caccgggaga atgccgtttt gaaagcagtt gttgtagaag atgtccgcga agctgggggc
     4021 gaggacgcaa cgaaaccctt gatcgagcag cgcccagggc gcatgttcgc gtgacgatcc
     4081 gcagccgaag ttgtcccgcg ccagcagaat cgtggcgttg tgatagcggg gttgattcag
     4141 gaagaaggcg ggatcctgcg agccatcctt cagccgccgc caatcgtaga acagtccttc
     4201 gcgcaggccc gtccgcttga tggtcttcaa gaactgtttt ggaatgatct gatcggtatc
     4261 gacattgagc cggtccaacg gagcgaccag tcctgtgagt gttgtgaatg cgtccatann
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac caccatggca tgcacggtgc
     4441 tggcgacttt tttccccttg gcgaaggagg cggccaggcg gaggtcttca atcctggaat
     4501 tggtacagga tccgatgaat accttgtcga tcttgatgtc gcggatcggc atgccgggca
     4561 gcaacgccat gtattcgagg gcttgttccg tcgcctggcg gagtttgtcg tcccccatgg
     4621 tgcgagggtc cggaacgttc ccgtccactc cagtgaccat cccagggctg gtgccccagg
     4681 tgacttgcgg ggcgatcgtc tcggctcgca actctacgac ggcatcgtat ttcgcgccgg
     4741 gatcggtggc gagttgccgc caggccgtca cggcctgatc ccagagggcg cccttgggag
     4801 ccatcgggcg gcctttgata taggcaaagg tagtgtcgtc cggtgccacc atgccggcgc
     4861 gtgctccacc ctcgatcgac atgttgcaga gggtcatacg gccttccatg ctgagcgcgc
     4921 gaatggccga cccggtgtac tcaatcacgt acccggttcc gccggccgtg ccgatttttc
     4981 cgatgatggc cagaatgaca tccttggcgg agcagcgggg cgacagttgc ccgtcgacac
     5041 gaatttccat ggtcttgggg cgtttctgaa ccaggcattg cgtggccagg acatgttcga
     5101 cttcgctggt gccgatgccg aacgccagtg cgccgaaggc tccgtgggtc gaggtgtgcg
     5161 aatcgccgca tacgatcgtc atgcccggca gcgtgaaccc ctgctcaggg ccgatgacat
     5221 ggaccacgcc ctgccgaatg tcgttcatgc cgaacatcgt gatgccgaat gtgttgcaat
     5281 tatcgtccaa cgtctgaatt tgtttggcgc tgatcgggtc ggcgatagcc acgcggcggt
     5341 cggtcgtcgg gacgttatgg tcggggacgg ccagcgcggc gcccggcctg cgcggagttc
     5401 ggcccgccac ggccaaccct tcgaaggcct gcggtgaggt cacttcatgc actaagtggc
     5461 ggtcgatata gagcaatgtg gtcccgtccg gttccgcccg aaccacgtgg tcgttccaga
     5521 ttttttcgaa taacgtctgt gcagccatgg cgatactcct tgtagacccg cgccgttgca
     5581 agtgattgaa ggcgcaggcg cgatgcgtgc ctattatata tgcgagcagg cctatggcat
     5641 caagggcaaa ggggggccgg aagaaattgc ctctttagat tttaaaaccg aggaccgata
     5701 aggttggcat ggaacaggaa ccggtcgacg ccgaacgcat cacgattccc cgctggacga
     5761 tcaaacgcga tccccgggcg ctgggttgca cgatctgtgg atcggcgatc gtgaaaatta
     5821 ccgccgaatg gcggcaggtt tcgttctgta cttgccggtt gatcgagtat cgtgtgctgc
     5881 cgcgcgctcc caaagcgctt cccgccccgt ccacggcagc agacgtacgg aaagaaccct
     5941 aatctcgctg agtgtctgac aatcttcgat ccctgccagg cttgtcttcg ggcaggctcc
     6001 gggcatgggg ccggagagat catttctgaa acggaggatc gtatggcacg atggcttgga
     6061 gcgttcgtgg tagcgggctg tctgggtgtg gccggttggt gtccggccga ggcgaaggac
     6121 caccctgcgg caaagtcgct gatgcgaaca ggacttgcca ctcacggaca ctcttcacgg
     6181 acctgtgcgg gcgacgatct gcccggaaat tggcacctgg ttgccttcga ctcctcgtat
     6241 cggttcagaa acccacaggc tccatatctg tttcctcatc aagcctttca atactcgcat
     6301 cgcggcggag ccaaatcggc ccactcgctc cagcccctct ccggcgatcc ggaccagctg
     6361 tttgaggcgg tgccgttgga catgacctat cgagttgaag gaaaaggccg cgtcgtgctc
     6421 aagacgaggg gacacactga gacggtggaa acctggacct gcagggtcat cacccaagat
     6481 cctgaggcga tgggagatgg aaccaggctg caacgcggtg atctggtcat gaccttgata
     6541 ggcagcaacg ggcaggggtt gtttgtccgt catttaagaa agcgcgccgc ataacctgct
     6601 tggcatggag tgaggaggcg tgctactacc ggcaagtcag tcatgcgccg ggcacaatgc
     6661 atcgacctat tccgccgagg aggccatgga cgatccgaat caagtgtgga gtcagccatc
     6721 ggtcgtcgaa tggtcccagc tgctattgaa cagttatcgc cactgggtcg ggcgggactt
     6781 gatgctcagg accggagggc ccgaggagca ggcccatgca ttgttcatgg cgccttttgt
     6841 ggtggtctcg cacggtgcgc aggaggatcc gatcctgaat tacggatcgc atctcgcgct
     6901 gaccttgtgg gagacgacgt gggagcagtt cctgcagact ccatcccgcc tgacggctga
     6961 agctgtgaat cgtgccgagc gggagtggat gcttgaacgg gctcgcgtgc aggggtatgt
     7021 ggataattac cgcggggtca gaatttcgaa cagcggacga cggttcctgg tcgaaagcgc
     7081 gattgtgtgg agcgtgatcg accaagccgg gcgtcgtcag ggacaggccg cgaccttctc
     7141 acgctggacc ttcctctaac cgtcggaatc gagggacgac ggtctcgccg atggtcaccg
     7201 attcgagaaa cattcccagc ggtctgatcc acagcccgcc tgctccatag agttgccgat
     7261 agacgacata ctcctcctcg gtctcggaat gccgagccac gcccagcacc tcatagtcgt
     7321 tccctttgta gtgacgatag cgaccaggtt tcatcatagt gtatccttgc gggttcatgc
     7381 ctgcgggaca gtctatcgga ccgggaatgt catcacaact ctcttacatg cctgaacggc
     7441 ggcacgatgt cgtggtgatc ggtggcggat cggccggcta tgccgccgca cggacggctc
     7501 gcgacgcggg cgccgatgtg gccatcgtgg atcagggtcc gctcgggggg ctgtgcatcc
     7561 tgcggggttg catgccgacc aaaacgattc tgcggtcggc tgaaatcatc gcgctgatga
     7621 agcgcgcgcg cgagttcggg ctgagtccgg tcactgccca agcagatctg tcggctatcg
     7681 tcgatcgcaa gaaccgattg gttcaagagt tcgccgacta ccgtatcgcc gcactgcgag
     7741 acccgcgctt caccttgtat gagtcccgcg ccgagtttct ctcaccccac gaacttcgtg
     7801 ccggagatgt tcggattcgc ggcggcgcat tcgtcattgc caccgggtcg agtgcggctg
     7861 aggtggccat tccggggctg gacgaagccg gttatctgac cagcgatgag atgctcgata
     7921 ttcgtcgcca accggcgtcg ctcctggtgt tgggaggcgg cctggtcgcc gtcgagttcg
     7981 ctcaattttt tgcccgaatc ggcacaaagg ttaccatgct gcagcgtagc acaaccttgt
     8041 tgtccgacat ggatgaagac atcagtcagg cgctggcggc ggcctttcag gatgagggga
     8101 tcgaggtgat gaccggcgtg tcgttccaac gtgtgagctc aaccccgtct gagaagaccg
     8161 tgcactttgt gcagggcggg gctgtccata cgcgatcggc cgaggttatt tttcaagccc
     8221 tcggtcgtcg tcctaacctg aaagggctca atctgaaggc cgccggagta tgcgtggtcg
     8281 atggccggct ggtggtcggc ggggatatgc ggacttctca accgcacatc tttgctgtgg
     8341 gtgacgtcaa cgatctcact cccattgtgc atctggcgat tgaacaaggc gaaacggctg
     8401 ggtttaatgc gacgcatccg aatgagcctg cgcgggtgat cgatcaccgg ttggatgccg
     8461 aagtcgtgtt caccgaacct caggttgcgg ttgcgggaat gagtgaacgg cagtgccgcg
     8521 aagaggcgat tccttatctg acggcgtcct atccgtttgc cgaccatggg aaggccatgt
     8581 gtctgggcgc agtgcatgga tttgtgaagc tgctctgtcg accggacgac ggcgcactgc
     8641 tgggcgccca gattgtgggg ccggaggcgg gggaattgat ccatgagctg atcgcggtga
     8701 tgtactttcg cggcaccgca caggacctgc tgcgcattcc ccactatcat ccgacgctcg
     8761 cggagatcgt gacgtatccg gcggagtcca ttgtcgagca gatgggtcga gcatgaaacg
     8821 aaccgttgcc gcgggtgcat cggcgcttct gctgctggtg actctggcct gcgggcagca
     8881 gacgtgggat gctgcgatgc aggcaggtga aacggcattg cagcgagggc agtatgagca
     8941 ggcggaaaaa attttctctg ccgccgtgca taaggcagaa gagttcggcc tacatgatcg
     9001 gcgggttgcg gtgaccttgg cccatttggc ccaagcctac agcgcgcagg gaaaattcgt
     9061 cgaggctgaa ccggtgtatc tggaggcgct caagatttac caggatgtgc acggtgagaa
     9121 ccatctcgat gtggcggcca tgttgaataa ccttggtgtt ttgcatcgga agcacggaca
     9181 atatgccgat gcgcagcgcc tcctgacgcg ggcgctctcg atcaaagaga agctcctggg
     9241 aacggaccat ccggaggtgg cgctggccct gagcaatttg gccgcgatgt atctggcgca
     9301 aggcgacggg gagcaagcgg gggccttatt tgcgcgggcc ttggcggtgc gggagaagca
     9361 tttggggccc gaccatccgg atgtggccaa aaatcttgag gactatgcga gtgcgctgcg
     9421 caagctgggg cgggtcggag aggcagaagg gctcgagcgg agggcgggcg cgattcgggc
     9481 aaaaccgaaa tcgtgatggc ggggggcgca gagttgaacc gatgggatgc gccggtgtag
     9541 cgtacagatt cgcaagggcg tgagggcgaa ggatgtctga tttacggacc aacgcattga
     9601 ttgcgcgcgg catgggtatt gtcttcattg ccggcggctt tatgcttggt atttacggct
     9661 tggaatttcc caacaccatg tggcttcaaa ctgcgttggg gctgctcgtc accgggatga
     9721 tggcgcaggg ctatgcgatg tattgttcaa tccggcgggt tcgggaacag cgtgattcgg
     9781 aacggtagcc agttgagacg cgagacgaag aaggtaatgg tgtgcccggg tcgctggagt
     9841 gccctcctgc tcctgctgtg tgtcgcaatg ttgtccggtt gcggcgggcc ttggcgtgat
     9901 cggtacttgg acaagggggt gaagaagctc acgcaggacg atattgcaga aaaattcggt
     9961 cctccgagta cggctaagac cccggtgctg ggtggtgaca ccgtctggac ctatcgcgtg
    10021 ccgatggggg atcgagaagt gcatccctgg gatcccggta gcctgaccgc cggcaagaaa
    10081 accaaagccc cgccctcgcc ctcgccgttc ggaaagagcc tggccggtaa tgacgaaccg
    10141 taccgggagc cgctctattg ttatcgctat acgctgtcgt ttgatgaggc caagttgctg
    10201 aaagattgga aacgggaaga atgtgttcct cgcaccagtg aggatgagac cgcatcccaa
    10261 taaggcggac acacgacatt atgcgcgaac tgttgccggt gatcggatcg agcgtcttct
    10321 tcgaccttct gaagtccatc ctccttctcc tcattctgct catcgcacgc acggttctgg
    10381 tccgttggat caaaggcaat cacacgttga ccatcgacgc gaagcgacga tggatcgtga
    10441 cggcgcgcaa cagcatggtg ctggggctct ttgtggggct cgttgtgatc tgggcccacg
    10501 agttggaagc gtttgctgtg tctctggtcg cgctggcggc taccgtagtg ctggcgacaa
    10561 aggaattgat tctctgctgg agcggggcgg ctttgcgtgt cggcgggggg gtgtacggtg
    10621 tgggtgatcg tatccagctg ggcacctacc ggggcgttgt cctggaatac gacgtctttg
    10681 cgacgaagct gttggaaatc gggccgggtc agacatcgca tctctacacc ggtcgcaccg
    10741 tggtctttcc caacagtctt ctgtttggaa atcccttgat caaagagagc ccgtcgcagg
    10801 aatacggcct ctacgttttg tcggttccgc ttcagagcac ggacgattgg caagccgctg
    10861 agcaagctct gctcaatgcc gcaaaagtcg agtgcgcctc cttcatggat caaatgggcc
    10921 gacagatgaa attgctggaa cagcgaaacc tgctggaagc gccgtctcct gagccacgta
    10981 ttaccatcca actccctgag gcaggacgta ttcatctggt gctcaggttt ccggcccccg
    11041 accgtggtcg ttcacgggtc gaacaagcca ttctcaggcg ctatctcatc gccgtgcggc
    11101 cgtaaggtct tgcgacgatc cttccagttt cttgccgaat tttgtgaagc ctctgccgcc
    11161 ggcttgtcct gcttggattc gcggtctcgg caaaaactgg caaaacaatt ggcaaactcg
    11221 ccccttcacg gtattgatga gtcctgcatg acggccggtt cgccatagag ccggacacca
    11281 tatttcaacc aagggggagt tgttgatgag aaggtcaatt gctgtgttgg tcgccgtcgc
    11341 atttgtggga atgagttcat tcgctgtggc cgatgatatg gggctgggta agatgtccga
    11401 ggggatgaag caggacatga gcggactgtc gaacgacctg aacgctcaga aggaagactt
    11461 ggcgagtgac ctgaaagcca agaaagaatc ggtgaagagc gagatgaagg cgaagcggga
    11521 cgaggcgaaa gcgaagaaaa agcaagcgaa ggccgacttg aaggcgaaga aggagcacat
    11581 gaaggccaag gtcaaggcca agaaagacgc ggcgcgcgcg aaggcggcag aagcgaggag
    11641 tgttgtcgac gatgcggctc acgaggccgg cgggatgaag aacgacgtga cgggggcgat
    11701 gcggaactaa ggtcggccgg ttggctgaga gccggataac gctgatattc gacaattgcg
    11761 gcctgtgatc gggaaatgtc cgatcacagg ccttgttgtt ctgtatgctt cagttgttgc
    11821 ggcgggtggg ctctgctaga atccccgccg tatgaaagtc gccaccttca acgtcaactc
    11881 gctgcgcaag cgcctcccta tcgtgctgca gtggctcgaa cgccatcagc ctgatgtgct
    11941 ctgtttgcag gaaacaaaag ttcaggacag cgaatttcct ctgactgcgc tcgcgacctc
    12001 cggttatgag atcaccttcc ggggcatgaa agcttacaat ggtgttgcga ttctcagccg
    12061 cacgaagccg gaagcaatct cgtatggatt cgatgacggg ggcgagacgg aagatgcacg
    12121 tcttctgcga gtggtgattc agggcatccc gatcatcaac acgtatgttc cgcagggttt
    12181 cgagatcgat tcgcccaagt accaatacaa actgggttgg tacgatcggt tacggaagca
    12241 tttcgaaact catctctccc cgaaggagcc ggcgatctgg tgtggagaca tgaatgtggc
    12301 cccaagaccc attgatgtcc acagcccgga gaaacatctg aaacatgtct gctaccacga
    12361 agacgcgcgc aatgcctatg ggaagacggt tgcgtggggc ttcgaagatg tgttctgcag
    12421 actgtatcca gatcgccagc agttcacgtt ttttgactac cgtgcaccca gtgcccttgc
    12481 cgccaataaa ggatggcgga tcgaccatat tctggcaacg ccgcccttgg ctcagaggtg
    12541 tcagctcgtt gctgtcgatt tagagccacg ccgtgcgacc gatccttctg atcacactgt
    12601 cctctgggcc gagttctcca cctagcaatg cattgaacag gcttgccgga tcggccctcg
    12661 cctcgttcgg acgtcgaacc tactgagtag gtaatcagtt ctctctatct tcatcgtcgc
    12721 tacggccggt ttgaaggccg cagcgaccat tctactcgtc caccaccgcc gaggaccggg
    12781 gcgtttgtca ggacggtcct gcgactcccc gattactgca atactgcgac tggctccaat
    12841 aacagttgtt cagcgagccg agttcgacgg ttttgttcaa tcagtggatc gacaatcgat
    12901 ccgcaattaa tgcaacgcca cacaaacgtt tccggcgaaa agtccggcct ccactcatcg
    12961 accattaacc ctttgcatcg tccgcatgtc atggttccca cctccttagg attgcgccct
    13021 tattatctaa aggccgtgcc catgtgagga gagagtagca acgcgagatt aaagaggtgt
    13081 tagtgagacg ttaaaaccat ttaatcctga ttgtgtcgaa atatcagggc gttcacattt
    13141 cacggttgga caaattcgga gcgaagggga agcgttctgt acaaaatgag gtgtgcgatg
    13201 agagttgtgg cggcgatgca cgctcggaac atgacgagtc acagggtcaa ttcggcgaga
    13261 gccgaaatcg gcagatctac gctgactggc ctctgaaaag agaagggcta atcagtgaat
    13321 ctgccttcct tctggagcga accgttgagt ttcttgacag aactcccccc atcctttatc
    13381 gattgaactg acagcgccat tccgctgcgc ctacggccgg tggtgtacag actgtgggaa
    13441 gttgctgtga tctgtgtccg cggtcggttt tgcctcaagg ccatctgtgg cgagtattca
    13501 ggtggagccg tgaccggcgt ccggcagagt atcttgtatt cgggagagcg gcgctaacat
    13561 ccgccgatca ttcagcatga tccaacccct cacgtgtccg cagtgcggcc aagacaatgt
    13621 gtttcgttca cgctgctcca cgctgatgga gcggttgcag tcgcttgctt gtattgcccc
    13681 gttccattgt cagtcttgta gcttccgttt ccaggccttc cgattgggac gccgttatcc
    13741 ggcgcagatg ctcgatcggc gaaaacatct gcgtattccc gtgcgcctgt ttttgtcctt
    13801 ctctggcggc aaagtcaggg gcgaaggaac cgtcttggac atttcgatgg gcgggtgcat
    13861 cattgagagc caagctccgg tgcatatgga cgatatcttt tatcttcaga tcgcgcttga
    13921 gaacgaccag acgccgctcg aagtggctgc gatggtccgg tctgtgagtt cccgcggtat
    13981 tgcgttcaaa tttcttcggt cggcgcaaga aaacaagcga cttcttgcgt ttgtgcatgc
    14041 caagactgaa tcttccggag caggttccgc cagctagctg tatcaacctc gcctatttcc
    14101 tcaggtgctc cgctccagtt tgcctagagc cgcccaatca nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn ctcttcaatg ttctgttaga gtggagtttc ccaggcggag
    14281 gcctggggat actcagtcgt aaggcggtcg tgttcgatgt ggtcgaaggg ttccacgatc
    14341 aggtctgctc ggttcaatgg ttcggggcat gccggtgagg agagaaaatc gacatagtct
    14401 tgtgccttcg cctcagtggc aaaacggcag agcccatact ccggcatatg agaagatgga
    14461 tgccaaaagg ccagcccgat ggagctgcct tgaaacaccc ccaaggctcg atgccggact
    14521 tgaaaacccc ccggagtatc ggtcgaacga tcgagcgcca tgccgcttcc cgttgtgtgc
    14581 tgatcgacta agaatgggca agtatagcaa ggttataaag gaatcgacat ctcgcgggtc
    14641 ccgccggcga ttgacttcgc cggtgaaagc cttacgttct atggatgcgt ggtccgctac
    14701 gaccggcgcg aaggaggaga gcatgttcat gtcattgacg cgttcgatgg ggggagtcgt
    14761 gctgcttgcg gccgtgctgg ctctgccagg ctgtggctat aacgacttgc aaggtcttga
    14821 tgaggatacc aaggcggctt ggagtgaagt gatcaaccaa tatcagcggc gggccgattt
    14881 gattccgaat ctggtgaata cggtgaaggg atatgccgcg catgagaaag agacgctgga
    14941 aagcgtcgtg caggcgcgtg ccaaggctac cagcgttcaa gtgacgccgg agttgctcaa
    15001 ggacgaggcc gcctttaaga agtttcaaga ggctcaacag ggtctctcga gtgcgctggg
    15061 gcggttgctt gccctggccg agaattatcc caacctcaag gcggatcaag ggttccgcga
    15121 tcttcagagc caactcgaag gtactgagaa tcgcatcacc gtggcacgca agcggtacat
    15181 cgacaaggtt gcagaattca acaagatggt tcgctatttc cccaccaatc tcaccgccaa
    15241 gtttctcctg catctggagg agaaggccaa tttcaccgtg gctgacgaaa aggcggtggc
    15301 caagccaccc gaagtaaaat tctaacgacg gtctcgtgag tcagggagcg attgcaatgc
    15361 ggctgatggg gcgaacgggg tgggggctcc tcatcgggtg gctctggctc acaggggtgg
    15421 cgagcctccc agctgcggcg ctggatgtgc cactcctcag cggccgtatc gtggatcagg
    15481 cgcatgccct gccgcctgcc gtcgcagagc gactctcagc cgatctggcg gcgcacgaag
    15541 ccaacacgtc caatcaggtg gcggtcctca tcattccatc gttggaaggt gagccgctgt
    15601 ttgatttttc ccatcgtgtc gcgacggcgt ggaagttggg ccgcaagggg accgacaacg
    15661 gagtcctcct tctcgtctcg atcaaggatc gaaaaattcg catagaggtc ggttacggat
    15721 tggaaggggt gctgacggat gcaaggtcgg ctcagattat ccggaacgag attgtaccaa
    15781 ggtttcgagc cggagatgtc ccggccggcg tgacggccgg tgtgcaagcc atcttgcaaa
    15841 ccattgaagg gacctaccgt gcatcggaac atccgacggc tactccttct tcgagcgatg
    15901 ccttcgccac tgcggccttc gcggtgattc tgggagtcgt ggttggtctc ttcttctcac
    15961 gcgctcatcg agtgctgggg cctgttgtcg ggggaggcct ttcgtttctg gctgccccat
    16021 gggtgatacc ggcagtgatg gccggggccg tgagcctggt tctggtcggc ctgctgagta
    16081 agctggggcc aagcagcgga tcacgcaggc ggggagatgg cagtgcgttt gacgggacct
    16141 ggttcagcac gcaacaaggc ggatgggggt ccggtggttt cggggggtcg ttcggaggat
    16201 ccggtggctt ctccgggggc ggaggcgatt tcggaggagg aggcgccagt ggcgactggt
    16261 agacggccga cgctgacaga tgaagagcgt gcgcgggttg aggcggcggt gcaggcggcc
    16321 gagcaacgga ccagcgctga aattgtgccg atgatcgtcg ggcgctccgg tttgtatcgt
    16381 gaggcgcacc atcgtgccgg gctactgagt gcggtactcg ccctgacggc cctgctgacg
    16441 atcgaaacca cctggctccc atgggggtgg catgcggcga atgccgtctg gttgcttgga
    16501 gtgatcatgc tggcctatgc cctcggttcc tgggtcggga catggcctcc tgtgctgcga
    16561 ctcttcacat cccgcgagcg gatgcatcgg aaggtgcagt tgcgggcgaa attagcgttt
    16621 gctcagcatg gtctcgcgca gactcgtgag cgaacaggac ttctgctgct gatctcccta
    16681 ctcgaacggc aggtctctgt gtcggcagac caatcgttgc gcgatgggat ctcggatgcg
    16741 cagtggcagg aggtcgtcgc cgtcgtcgtc gaacgactga aggtcggtga tctggtcggc
    16801 ggcctctgct gcggcatcga agccagcgga acgctccttg cgcgcgcctg tcctgcgcgt
    16861 gacggcgaca atcccgatga actgtcgaat caggtgattc aggacacgtg atcgctggtt
    16921 cttgccggac gatggacggc tccgttcggt ttcgctacaa tcccgattaa tgtcggatcc
    16981 cgccgcctcc gttgaaccat ctcgaccagc tccggatgaa acccgttccg tcgtcaaggc
    17041 ggcgggcctc atcggcgtag cgaccttttc cagccgcatc ctcggttttg tccgcgacat
    17101 ggtgttagcg agactctttg gcgcgacccc cgccgccgac gcgttctttg tcgcataccg
    17161 catccccaat ttattgcggg aactgttcgc cgaagggtcg atgtccgccg ccttcattcc
    17221 ggtctttacc gaatatcaca cgctgaagac caagcgggat gcctgggaac tggccagcgc
    17281 caccttcacg acgctgctca ccatcgtcac cgcagtgact ctgctgggga ttctggccgc
    17341 gcccgggatt gtctggctcc ttgcgccggg ctttcgcggg agcccggaca agctggcgct
    17401 gacgacgctg ttgacgcaga tgatgtttcc ctatctgatc tttatcagcc tggcagccct
    17461 ggcgatgggg attctgaact cgctgcgcgc ctttgcggcg ccggctttct cgccggtttt
    17521 tttcaatatt ttcaccattg cctgcatgat gttcctctcg ccctggttgc cggagcccat
    17581 catcggcgtg gcgatcggca tcgtcgtcgg aggggtggcg caatttgcga tgcagctgcc
    17641 gggtttaaaa ggccgcggga tgttgttcgg cctgcggttt cagcccggtc atccgggtgt
    17701 gaagcgcatc gggtggttga tggtgccatc gttgctgggg ttgtcggtga cgcagatcaa
    17761 tatcaccgtg agtacaatcc tggcctcgta tttcgccggt ggcccgacct atttgtttta
    17821 cggcatgcgg ctcattcagt ttccgctcgg cattttcggc gtggcgctcg cgacggcgat
    17881 tcttccgacg ctctccgcgc aggccgcccg tggggcgctt gatgaattgc ggacaacgat
    17941 cgggttcggc ctgcgaatga tttttttcat cattcttccg gcgatgttgg gtctgatcct
    18001 cttgcggcac ccgatcgtgc atctggtgtt cgagcatggg tcgttcacca aagccgatac
    18061 attggcgacg gcgacggcgc tcctctgtta tgccgtcggg ttgtgggcct ttgccggagt
    18121 gcgtatcatc gtctccgcgt tttattccct acaggatacg aggacgccgg cggtcactgc
    18181 ggggattgcg gtcggggcga atattctgtt gtccttgtgg ttgatgacgc tgctggatgc
    18241 cgcggggctg gctctggcca cggcactggc ttccatgctg aatgggagta tcctggtcgc
    18301 cgtgttgcac cggcgtctgg ggcgtgtcga ttggggttcg attgtatggt cggcttttcg
    18361 agtgctgggg gcctgcattc cggtcgcagt gatgtgcctc tgggtcgcga atgcgtccct
    18421 gtgggaccag gacggtgcgt gggtcgtgaa atccgcagcc ttgtttggtg gaatcggagt
    18481 cagcgtcatc ggatatctcg gggtgcatat tatgctcggc tcggaggaat tggacgtggt
    18541 gttaggaatg gtgaagcgga aattgggacg agtggcgaag aagtttgggg cggcctgaca
    18601 tgacgcacgc aatcacgttt cagaccgctt ggggcttgat caggattacg gcttccgaga
    18661 agggcgtgac ctccattgat ttgtcgccat cggatcctgc tgcccggcag caaccggctg
    18721 aggccggcgg cgcagccgca tcgatcgtgg aagaggcgcg cgcgcagctt gtggcctata
    18781 ttgcaggcac gcgtcgcgag ttttcatttt ctgtggatta ttctgccggc acgccgtttc
    18841 agcggaaagt ttggaaggcg atcacacgga ttccctatgg ccgggtgcgg tcctatcaat
    18901 gggtggccat gcgtgtgggt ggaaagcagt atgccagggc ggtggggatg gcgctcgggg
    18961 ccaacccggt ccccatcgtg gtgccctgtc accggatcat ctcgcacgac ggttcgctag
    19021 gtggattttc ttgcggattg ccgttgaagc gccgcctgtt gagtttggaa ggcacgttga
    19081 atcaactgcg gaatgctcag tgattgtggc tggttgtcgg tctgtgaagg atgatgtatg
    19141 aaggttgtca ttcaacgggt cacacgcgcg tcagttgaag tggagggccg gatcattggc
    19201 cggatcggcg caggtctcct ggtgctgttg ggtgtcgcga agggggatga cgagcgcgac
    19261 ctgctgtaca tcgttgagaa gcttcagacc ctgcggattt ttggcgacga gcaggggaag
    19321 atgaatcgta cgctcatcga ggccggcggg gcgctgttgc tcgtctcgca gtttacgctc
    19381 ctcggcgata ccagcaaggg ccggcggcca gggttcgacc tagctgccct accggaggag
    19441 gcgcgtacat tgtacgagca ggcggtcggt cggctgcggg cgaagggtct gacggtggaa
    19501 accgggctct tcggggcgca catgcaagtg gaattgctga atgatggccc tgtgacgttc
    19561 ctgttggaca gccggcgagg agcatcggct ccgactctca agtcttgatc gcggatgccg
    19621 ataaggaatc agggagaaag tctgcttggg ttgagcatgg tctgctatac tattttcaaa
    19681 gggcagggat tgcccgggag gtcgagatgg gaagtcccgt ttctcgcagc catccactcc
    19741 gacagctctt cggagcgttg acggaaaaaa gcttcaccga acacctcggg tggcccgatc
    19801 tgaatgtcac cgagtacgtc tccaacctgc tcgtcgaatt cgctcacacc gaccaactct
    19861 acaaaatcca gaatacacag ggccgcgccg tcgattccgt cgtcgagatg ctgttcgaat
    19921 ccgaagtgct gttggaagcg caatccttcg agcgggagcg cgaagtgcac cgccatatcg
    19981 gcgactttac actcttcatg accggcctgt ttccagaata tctccgccgc ctcaagagcg
    20041 tgggccgcat ctaccataaa gattttctgg tggattacgt gaagacgggc aaacgctcgt
    20101 acgggttggt cgccgagtac ggcagctacg accctgagca ggattccccg ttgttccgga
    20161 agctgtcgga aaatttcgag ctgtgtgtga ccggtttggg gttcgtccgg tcggacctcg
    20221 atcgtatgca agatcctgcc tgccgccgcg tgaaagatct cctgctgaat tgaaaccatc
    20281 acgaccgatc ctgctcattc acggtggcgc gggacgccgc gccatgaccg tcgctcaagc
    20341 cgcctgcgtc gagaccgcct tggccgaggg ccaccgacta ctcatggccg gaaccccggc
    20401 gctgtcggtc gtggaagaag ccattcgcct gctggaggag tcaggactgt tcaatgcggg
    20461 gcgaggctcg aaccgtcagc tcgatggagt gcggcgcatg gacgcttcga tgatggaagg
    20521 gcgcgatctg cgggcagggg ctgtggcctc catcgagggt atcgtccatc ccatcactgc
    20581 cgcgcggctg gtgatggaaa aaacggccca tgtgttgctg gtcgggcaat cagcctcccg
    20641 ctttgcggac tatttcggat tagagcgcgc gccccgtgcc aagaggacag cagggccaca
    20701 gcccaaggac ttgaggccgc gggccgggct gaagcacacg ttgcgcttgc atcaggcgat
    20761 catgcagacg ggccgcctcc gtgcgcgcag cttgggcaag gaaaccgtgg gcgcggtggc
    20821 cttggatctc gacggaacgg tggctgccgg cgcatcaacc ggcggggtgg atgtgatgtt
    20881 gccgggccgg gtcggcgata cgccgttgat cggatgcggg gtctatgccg ataatgaggc
    20941 cggagcggtc tccatgacgg gattagggga gagcatcatt cgagtggccg ttgcgaaaga
    21001 gatcagcgat ttattggcga gcggcctgag tccgctgcgg tcggcgaatc gggtgcttcg
    21061 gaaggtcgta gagcgtattc acggcacggc cggggtgttg gtgctctcgc cggatggccg
    21121 gttcgccatt cgacacagta cgccgaacat ggtggccggt gtgatcgggc gcgacggccg
    21181 cccgcacgtg aaaggtcggt ttgcctagtc gcgcaagagg ctgatacagt gaccagaggt
    21241 atcagcgtat gccggcactc tttcacctcg catttcctgt ccacgatctc gccgcagcca
    21301 agcaatttta cgtcgagggt ctcggttgtg tgctgggtcg ggaatcctcc tccgccgtca
    21361 ccttcggact agccgggcac cagttggtcg cccatctctc cacccagtcg acagtgccgc
    21421 agaagggcgt ctatccgcgt catttcgggc tggtgttgac gcaggaagaa gaatggcagg
    21481 cgctggccga tcgcgccaaa gcgaagggcc tgacgtttta ccaacagccc aggcggcggt
    21541 ttgccggtac ccgcatcgaa catctcacct tctttctcca agatccctcc cggaatctcc
    21601 tcgaatttaa atactatcga cacgagtcgg cgattttcgg ggaacgagac gtagcggcag
    21661 tcggggatgc tgagtagcga atgctctccg cagcatcgtg cggaccgtgc tggtgaagtt
    21721 atggctcagt tttcagaagg ggcgaggtcg tgcggctccg gtgcctgtcg gttcgtcgag
    21781 agtgagccgg ctctgcgtcc gttgccccgt tccagccatc ggacgatgtg gtgctgccgt
    21841 cgatttaaat aggcggtatg gcggatcggg tcataccggc gcggggaggg aagaatcgcg
    21901 gccagtagcg ccgcttcgtc gatcgtcagc tccttggccg atttcccgaa atggtgccgc
    21961 gctgccgctt ccgcgccgaa tacgccctgc ccccattccg cgacattcaa atacaattcc
    22021 aaaatccgtt ccttcgtcaa atgatgctcc agggagcgtg tgatcaaggc ctctcgggct
    22081 ttccgcagca aggaccgttc agaagagagg tacagatttt tcgcaagctg ctgggtgatg
    22141 gtactgcccc cgcgtttgaa ttcgccgacc tccaggttgt agagcgccgc atctttgatg
    22201 ccttcccaat cgaatccttc gtgcgcgaag aacgaggcat cttcggcggc gaccacggcg
    22261 cgctgaaggg cgggcgcgat tcgagccaga gggacccagg agaatttgat gtgcagagga
    22321 tggccttgtt ccttggcctg agcccgccgg gcttccatga ccgccgtttc cgtggggtga
    22381 tgtttggcca gatgggagac gtccggcagc gtcaccagcc aatacagcgc caaggcggct
    22441 gcggggaggc cgaccaggat ggtcgcccac aacagcattc ggccgaacag gctgccagtt
    22501 gacttgctca ttcccgacac tctatctatg aggatacgag gtgttgcacc ggtccggccc
    22561 ctgtccccgg aggtgaacgt acatcccgca cgtcggtacg cggtggatca ccatgaaatt
    22621 actcagcgct gagtttatca aaagttcggt ctccccagaa cagtttcctc ccgaccgatt
    22681 gccggaaatc gcgtttgtcg gccgatcgaa tgtcgggaag tcctcgttga tcaattccct
    22741 gcttcaccgg aagaagctgg ccaaggtcag caagacgccg gggaaaacac gcgccgtcaa
    22801 tttttttacg gtccgcacct cggatccgaa gctctccctg ctgtcgctgg tcgacctgcc
    22861 cggatacggt tacgccaagg tctccaaaac catgcgggcg caatgggggc cgatgatcga
    22921 gcagtatctc gaacagcgac agtccctcgg ggctgtgatt ctgttgatcg aggcacgtgt
    22981 gtggatgccg caggatgtga tgacggtaca gtgggtccag tctctggggt gcggcatgat
    23041 catcgtcctg acgaaagccg ataaattgcg gcaaagcgag cgggcgccca gcctcgttgc
    23101 cgtgcgaact gcttgcggat tggggcccga ggtgccgatt gtgctgtact cggctgaaac
    23161 gcatgagggg cgggacgcct tgtggggcga gattcgtacg cagtttaann nnnnnnnnnn
    23221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23281 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt aaaacttgat ctcgatgtag gctttgtttt
    23341 tgagatcggc catccagatc tggtactgat cctcggtctt ctgctgaaac acgagggatt
    23401 gaatttcggc cttcacctgt tcaaaaggcc gaaactgacg cggctttttg tcgtcgaccc
    23461 gcacgatgtg cagcccttcc gccgtctcca caatgcccgt gatctgcccc acatccacgg
    23521 atgtcagcgc ctgttccagc gcgggaatca actcgccttg tcgaaccagg ccgagccgcc
    23581 cgccgcgcga ggcatccgcg ccgtcggaaa agcgcagggc gagatcctcg aacggttcac
    23641 cttgcttgag ggttgccagg agcgcttcgg ctcgtccatg ggcggcggag aggccgtccg
    23701 gcgtgcgcgg cttgatcagg atttggctga gttggtattc ctcggggtag gcaaaccgat
    23761 cctggtgctc ctggtagtac cgtttcattt ccgagtccgc taccataatc aggccgcgga
    23821 cttcgcggtc gacaactcgc attaaggtca actgttcgcg aatacttctg gcggtgttcg
    23881 ggtcggcgct gtcgatggcc tcgccctgtt tcttcatctc ttctactgcc tgcacgactt
    23941 cctgatcgga cacgtccaca cctttgttcc tcgcggcctg cagctgcagt ttccgctcga
    24001 tcattttcgt gacgcccatg gcctcagccg tcttgaggcg gcgctccagg tcttcgcctt
    24061 tgtacagctt gcgcagccgg tcttgctcgg gaagcaggtc gcgcttcaat tcggacagca
    24121 tgatcaggtc ggaattgacg accgcgacaa tgcgatcttc cagcttggcg gcctccgcgt
    24181 tggcgatcag gacgaagagt aagcagaggc agaaaaagag cgcgcctgtg gtcgtgagcc
    24241 cggtcgtgct gcgaacgagt cctggggcag gcctgccctt ctgggacaag aagaaccggt
    24301 agccatggag gcacgtgtgg gcggacagcg attgatcgtt catagggttg ctggattgta
    24361 cacaatcgag gcttcagaaa gggagcgctc tggagatgaa aaacgcggac gggtgaagac
    24421 ggagagtatg caggagggga gtgcgcgggc ggttacgggc gcgctggttc atcggtcacg
    24481 taacgagctg cgtcggccag gcggatcgtg gcaccggtgc gaagttcggc aaacacgtcg
    24541 tccagccgtt tccggcgctt ctcggccaac agttcctggc ggagtcgttc gcgggtggcc
    24601 tggtctgcct gcagaatttc cggctccagg gggctgacct tcacgaggta atagccctgc
    24661 gcggtcttga tcggatcact gatgaccccg gggtgcagag aaggaatcaa cgcatcgagt
    24721 tccggttcca gcaggccttt tttatacggg cccagatcgc cgcccttggc gcggctgcgt
    24781 tcatcgatcg aataccgcag cgcgaatcgc gtgaaattgc cgccggcttc gacctgccgc
    24841 ttgaggtcct tggccgcata gacgttcggc agcagcataa tcgacacttg caccttgggg
    24901 ttggccagga gctgattggc gtgtttcccc agataggcat ccagttcctc tttgctgacc
    24961 tcgaccttgg tcttaatccg gtctttgagc aactcatcca gaatcagctg ctcgcggtag
    25021 cgcagggtct tttcgcgaat ctcatcggac tgatccaaac cctgcttgcg cgcttcctgc
    25081 atcagcagtt cgcgcatgat caattcatcc aaaaaacggc gtttcccgcc ttccttttcg
    25141 tagcgcgcgc gcgtggcctg cgacaggtct tcccagcgga tgtcgaattc ggattgagta
    25201 atggatcggc cgttgatcat cgcgatcacc ggctcttcct gcggtggttc ggtgcaggcg
    25261 gccaacgacc cgagcgcgca gagacccagc gcggcgctgg tcaatcgtgt ctgccaggat
    25321 gaaatctgtc gtgtgcgcgg tggcgtcatg cggtcggggc ggagcatcct ctacgaaggt
    25381 caggtcgagg ccgtgcgtgg cccttgattg ttggtaccac agacgtggag ggtttgcaag
    25441 gttgcgttga gttctggaaa gaccaaactc cagtcttcat gcggtatctg gagttcaaaa
    25501 gacagcggcg acagaaaccg tagtcgtttc ttgtatcggt ccatcagggc ttgcacggcg
    25561 gtttcggaga ccgtggcctt gggatcgaag gtgatcacga ccgcatgcgg ttgctcgacc
    25621 acagagctca gccggagttg cttggccagc agccggattt gcatgagctc gaacagacgc
    25681 tcgaccggat cgggcggatg cccgtagcgg tcctcgattt ctccgtgcat cagcgcaagg
    25741 tcgccgagtt gtccgcagga agacagtcgt ttgtagagcg acaggcgctg gtggctgtcg
    25801 gccacatagt cttctggaat ataggcggag acgctgagac gcagcgtggg atccggttct
    25861 tcctccacca cctggccctt caggcgttgg accgcctgtt ccaccatttg caggtagaga
    25921 tcgagcccga cggcggcaat atggcccgat tgctgtttgc cgagcagatt ccccgcaccg
    25981 cgaatttcaa gatccgctgc ggcgatgcgg aagcccgatc ccaattcggt aaactgttgg
    26041 atggcggtca gccgcttctg ggcatcttcc gagaggttgc cttcatccgg caccaggaag
    26101 taggcgtagg cctgttcgcc gccccgcccg acccggccgc ggagttgata gagctgggcg
    26161 aggccgaacg tgtcggcgcg attcacgatg atggtgttgg cggtcggcac gtcgatgccc
    26221 gattggataa tggcggaggc aatgaggata tccgcttccc ggtggaagaa tttcagcatc
    26281 acagcttcca gcggtttcga gtccatctgg ccatgggcca tgacgatgcg ggcttccggc
    26341 accagttcct ggagccaggc gcccatccgc tccatggtct ccacccgatt gtggacaaag
    26401 taggtttgtc cgcctcgacc gagttcgcga agaatcgcct cacggatcgc cttttcactg
    26461 aagcgaagga cttgggtgcg gatcgccagt cgtcccgacg ggggcgtatc gatgatcgag
    26521 aggtcgcgca cactcgccat agtcatttgt aatgtgcgtg ggatcggcgt cgctgtcagg
    26581 gtcaacacat cgacctgggt gcgcagctgt ttcaaccgtt ccttatgttt cacaccgaac
    26641 cattgctcct cgtcgatgat gacaaggccg aggttacgga acttcacgtc tttttggagc
    26701 agccggtggg tgccgatgag gacatcgacc aggccggctt ccgtatcttt gacgatcgct
    26761 ttcgtgtcct tcggagattg aaatcgtgac agcagggcga cgcgggtcgg aaagggcgca
    26821 aaccgttcgg cgaagttatc gtaatgctgg tgtgcgagca gcgtggtcgg caccagcacg
    26881 gccacctgtc gattttcctc gacggccttg aacgcggctc gcatcgccac ttcggtcttg
    26941 ccgtagccca cgtcgccgca cacgaggcga tccatgggct tggtcgaggc caggtcgttt
    27001 gcaatgtctt cgatcgcctt gcgctgatcc ggcgtttctt cgtattcgaa ggccgcttca
    27061 aactcgtggt agagcatgct gtctttaccg tacgaggtgc gatgaaccag ctcgcggttg
    27121 gcgtagagat cgacgagctc gtgcgccatt tcttcgatgt cttttttaac ccgcgcggtc
    27181 gtcttggccc aactggtgcc gcccaggcgg tccaggcggg gtacgtgggc atccgctccc
    27241 gcgtatcgtt ggacctggct gagccgatcg agcgggacat aaagcttgtc ggttccggcg
    27301 aattccagca ccagaaagtc gctgtcgaag tcctgcaccg agagccgacg cagcccctga
    27361 tacttcgcga tgccgtattg catgtgcacg acgtagtcgc cgatgttcag atcttccagc
    27421 gaggagagga aggtggcggc tttgctcttg ggctgcggtt tgtgccgggc gcccttcgcg
    27481 aagagctctt cttcggtcag gaccaccagt cgcagatcgg gagaaagaaa accagccgag
    27541 acttcgccgt tcaagactga aaacggcgcc ttttgcgcgc cgccggccga gagcgccgag
    27601 ggcttccatt ccacagccgg tcggtcgtgt tcgccaaata acgccagcag gcgtccgact
    27661 tggccttggc tgcgggccac caggacgacg ggtccgcctt cgcgcaagcg atcaagcatc
    27721 tccagcgtat ggctgaacgc ggtgccgcgt tggccgagtc ccacgctggc gggggtttga
    27781 gccgggcagg tcaggaccgg gtcccagctc gcgtccggtg cggtgaccgg ctcaagcgcg
    27841 agcgtggcgt agccttgggt cgcggcgaga atctggtccc aagtgaggta caggttgtcc
    27901 ggtgtcgggt agggattcgg atccgtgcga tcttcgtgac gcaggaatcc ttcttcgatg
    27961 gcctgccaac attcctccgt gtgagctttc agcgtaccgg gttgatccag aacgagcacc
    28021 ggcggctgag ggaaatagtc gaggaggctg tccatggtcc cgtagaccga tggggcatgc
    28081 cattcggcgt cggcggccag gggcgcgagg gcatcgggcg cgtcctcggg gcgaatcaat
    28141 tcgcgggccg gcagcaccca ggcctgtttg atcttgtcgg tcgatttttg tgtggccgga
    28201 tcgaagaagc ggatcgactc gatcgtgtcg ccgagaaact ccacccgtag cgggtcggca
    28261 taggcggtcg aatagatatc gacgatcccg ccgcgaatgc tgaactcacc gggaatttct
    28321 accaccgatc ccttccggta gcccaaccgc aacaggctgg agacgagcac ttcccgctcc
    28381 agggaaccat tcggttgaaa ctgcaaaatc gcgtcggtga aaaccagcgc cggcagcacc
    28441 cgctgcgtta aggccgggat ggaggtgaag agcaccgtgc gcgccgtggt gcagaggcgg
    28501 ttgagcgtct gcatgcggcg ggccaccaga tcgatgtggg gaactgtcga ttcatagggc
    28561 agcgtctccc atttcgggaa cagcgccagg tcgtctccgg actgtccgca gagcgttcta
    28621 aagaacagcg tatcgcgata gaggcgctct gcctcgtcat cgtttttggc gacgatcagc
    28681 caggaacggt cggccagggg ctgagacggc aggctgtgnn nnnnnnnnnn nnnnnnnnnn
    28741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28801 nnnnnnnnnn nnnnnnnngc tgtcgtggag cgaatacgtt gcaccagggt ggtcgtcgac
    28861 actccgggaa cgagtggaat ggtctggacg cggccgccgc gggcctcgac cgtctcccgt
    28921 cccacaatcc ggtcaacgga ccaatctcca cctttgacga gaatatccgg ctggagggtt
    28981 gcgatgaggg cgccggggtc cggttccgaa aaggtcacca catagtctac gcaggccagt
    29041 gcagccagaa cctcggctcg ctgggcatcg ggcacaatgg gacgatcggt gcctttgttc
    29101 agggcgcgca cggactcgtc ggaattcacg ccgaccacca gcacatcacc cagattgcga
    29161 gccgcttgca ggtagcggat atggccgatg tgcatcaggt cgaagcagcc gtttgtgaag
    29221 acgatgcgct gtccctgccg acggcgctct tccagtcgcg aggccagttc ttgtcgcgtg
    29281 gtgactttgg tgtgcatcgt ggtctcgcct gcgaaaaact catcttaaca ggatgtggaa
    29341 aaagtctgcc accacgaatc cacgacggcc tgaccgtaaa ccggctgtca gttttccgtg
    29401 cgacgtcccc gcttctttgg gctgtccccg ccctgtggaa gtcctcccaa tcgaaccacc
    29461 cccgcgccat gttgctggat caaccggtcg agtatggcga ccgcgtcgcg ctgccgccgg
    29521 tcgaacaggg gttcaggagc aggaaccaga tccgacagcc cgaggcccac caggcgatag
    29581 cgcgatcgcg gctgcagcag ttcggcgagg gcgaggcgga tgtccggcca catatccgga
    29641 tcgtagttca ccggtgacga gaacttccgt tgccgcgtgg tgatgcgaaa gtgcgaatcc
    29701 ttcagcttca cggtgaatga cccggcggcc agaccctcca gccggagatc gtgcgttaac
    29761 gtttcgagaa atccgtggat gatcggttcc agcaaggccg gatcgttggt gtcttcgtcg
    29821 aaggtggtct catggctcat actcttggcc tcgcgatcgg ccaccacggg atcggagtcg
    29881 tggccgcgag cgagtgattg aaacgccgcc agccgcgagc caagcaggcg gatgagggat
    29941 tgttcgaagc ggggttcgag cagatcgccg acggtgtgaa tgcccaggcg ctcgatggct
    30001 tcagtggatt tgggacccat gcccggcaag gagcgaatcg gcagcggggc gaggaacgcc
    30061 gcttccgagc ccggttcaat cacggcgagc ccgtcgggct tatgcgcatc ggcggcgatc
    30121 ttggcgacgg tcttgccgga cgccagggca atggtgcagg tcaggcccgt gacgcgggta
    30181 atgtcgattt tgagcgcctg ccccaactca cgcggatcgg gatgccgcgt ttgcaatcgg
    30241 gtcgtatccg cgtaaaactc atcgatactt gtccactcgg tttccgggaa aaaccggtcg
    30301 gtgatgtcct gaagctggcg gtgcagacgc tggtacagcg gccggtctgg tggtacgaga
    30361 acgagctgag ggcagagccg catggcctga atggtcggca tcgccgatcg caccccgaag
    30421 cgacgcacct cataactcgc cgctgccaca atgccgcgag gcggaggccc gcccacggca
    30481 accggtttgc ctgccagcga cggatcagcg agaacggctg ccgacgcaaa catggcgtcg
    30541 atgtcgccga agagaatttg tcggggccat cgtccggaca tcgaggtcct acacggtggt
    30601 gaagggaaag ccgagctgag gacctgcggc agatgtgtgg gccggttccg cagtggaggc
    30661 gggtaccacg gcaggcagtt cgcggagcgg cccccgacag gcgccgcaga agtggcgtct
    30721 cggttggatg gtccggcgtt gtcgttgata gaggcagccg cagtgctggc agcgccaggc
    30781 atatttggcc aaggcggcca cttcttttcc cagcgagtga tagatcgtga tgcccaggcc
    30841 gtcgcgattc atctggtcca tcatgcgacg aaaatcggca ccatgattcg ggcgacgctt
    30901 caagatatcg tactgccatt ggtgaatcat ttcgtgcgcc agggtcatga cggtttcgtg
    30961 gctcgactcc gggttgttgg gtttgagcag caccgccgac agtctgatgc aacgccgcct
    31021 ggttggtgcc tcggtggtgc gcgcgagtgg ttgtcggggt ccgatccggc agacaaacat
    31081 cccggctgag gaggtcagtc ggcggctcca ttcaatggga atcgggggta aggtgccctg
    31141 aaaatagcgg cggttgagat cggtccagat tgtgagcagc ggttccggag agggtgacat
    31201 ggcactcaca acatgatcgt gatgttgaat agtcgagcga aagctgaatg gccagagtca
    31261 gcggccggac aattcttcaa tgactgcggc ggccaacagc ggcaccatga tctcgtgatg
    31321 gccggtcaat gcgtatccct tgccgccttt ctgggttggg cggcgcacga cattggtcat
    31381 ggggcgataa tgcgtgagga aatccatatt gaccgtggtg atgttcgtca acggatgacc
    31441 aaggttgcgt ccgagcgaga cggctttcag gaagacctcc ggcaggatca cggccga
//
LOCUS       DAOQRM010000012        31216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_847_length_31216_cov_14.010526, whole genome shotgun sequence.
ACCESSION   DAOQRM010000012 DAOQRM010000000
VERSION     DAOQRM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 31216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31216
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_847_length_31216_cov_14.010526"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /gene="rnpB"
                     /locus_tag="PKK81_02320"
     ncRNA           <1..221
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKK81_02320"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            717..2495
                     /gene="ilvB"
                     /locus_tag="PKK81_02325"
     CDS             717..2495
                     /gene="ilvB"
                     /locus_tag="PKK81_02325"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNV31733.1"
                     /translation="MKLTGSEILIECLKAEGVKTIFALPGGVVLKIFDMLHQQKDIEV
                     VLTRHEQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSVPLVCISGQV
                     STSLIGNDAFQEADNIGLSRPCTKYNFLVKDVNDLATTIKEAFYIATTGRPGPVLVDI
                     PKDVSMAKTEFTYPSSVSIRGYNPTYEGNKWQIKQAAEAIMKAKKPILYVGGGVVFSQ
                     ASKELLELAEMTQIPVDMTLMGLGAFPGEHPQSMGMMGMHGTYCANMAVHYSDLVIAV
                     GARFDDRVTGKVSEFCPFAKVIHIDIDPTSIRKNIHVDIPIVGDCKAVLRELNQILRA
                     TVNGNQKDLRKPWWDQIREWQQAHPLAYQQEPDGAIKPQHVVKRLYELTKDRDPIVAT
                     DVGQHQMWTAQYFKLARPNRWLTSGGLGTMGFGFPAAMGAQAAFRDRLVLCVAGDGSI
                     QMNMQEMAAAVVSKLPVKVIILNNRFHGMVRQWQDLFYEGRYASSYLDTTPDFVKLAE
                     AYGAVGLRASKVGDLDTVLKEAIATDKPVVVDVPTYPYENCYPMIPAGGCNHEMILED
                     PPELKRRMAGAPSTGSDEDKDTILTA"
     gene            2537..3055
                     /gene="ilvN"
                     /locus_tag="PKK81_02330"
     CDS             2537..3055
                     /gene="ilvN"
                     /locus_tag="PKK81_02330"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNV31734.1"
                     /translation="MEHIISVTVENKFGVLSRVAGLFSGRGFNIESLSVAPTLDPSMS
                     QMTIVTSGDERIVEQIVKQLNKLIDVIKVVDLNESEFVSRETALIKVHTKAEDRAEAL
                     RIADIFRANVIDSTPSTYTIEVTGDPKKLEAIINLLQPLGIKELIRTGRVAIAREAIR
                     PALSQPKKIARE"
     gene            3123..4136
                     /gene="ilvC"
                     /locus_tag="PKK81_02335"
     CDS             3123..4136
                     /gene="ilvC"
                     /locus_tag="PKK81_02335"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNV31735.1"
                     /translation="MKIYYDKDADLQLIRGKKVAVIGYGSQGHAHSLNLKESGANVVV
                     GLREGSSWKKAEASGLKVMPVADAVKASDVIMILAPDEAQAAIYRQEIAPNLKPGSYL
                     AFGHGFNIHFGQIVPPADINVFMVAPKGPGHLVRSEYTKGSGVPCLLALHQDPSGNTR
                     QVGLAYASAIGGGRAGIIETNFREETETDLFGEQAVLCGGLTSLIQAGFETLVEAGYS
                     PEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDITRGPRVVTDQTKQEMKKI
                     LGEIQSGQFAKEWVLENQANRPVYNALLKKGEGHPIEEVGARLRSMMPWLKKDQLVDK
                     NKN"
     gene            4188..4838
                     /locus_tag="PKK81_02340"
     CDS             4188..4838
                     /locus_tag="PKK81_02340"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNV31736.1"
                     /translation="MADRAVGIPIVKEGLPFVGGLVGATLLFGAAGWIIPAVIAGGFT
                     LFTAWFFRNPSRTIPQQPNVVVSSGDGKVIAIEEEFEPRYLKEKSIRVTVFLNVFDVH
                     VNRMPCAGTVEGVSYQPGQFLVASKPEATLRNEQNAVMLKTESGAKVLCVQVAGLIAR
                     RIVCWVGQGDRVERGERFGLIRFGSRMDTFLPLGSKICVSLGDRVKGGETIVGELR"
     gene            4850..5635
                     /gene="pssA"
                     /locus_tag="PKK81_02345"
     CDS             4850..5635
                     /gene="pssA"
                     /locus_tag="PKK81_02345"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNV31737.1"
                     /translation="MRAPFAKGNRKRQAMYLIPNLCTTGNLFCGVFAILSVFNGQHLV
                     AAIAILVGMIFDMLDGKLARLTNSTGQFGIEYDSLSDVVSFGVAPGVLIYSYALSGQG
                     MFGVAVMFAYVAMGAVRLARFNATVSTSDSKYFTGLAIPAAAGVIASLVIFDLHITQM
                     GSEVKPLLILVITFALAFLMVSTIKYRSFKDMKFRRGDHFTYLVWGILALMLIVAWPQ
                     AMLFVTFTGYAVSGPLARLWTMVSKSAGKPVAKTDTPVLDSRE"
     gene            6053..7600
                     /locus_tag="PKK81_02350"
     CDS             6053..7600
                     /locus_tag="PKK81_02350"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNV31738.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNVEEKLMIAKQLARLGVDIIE
                     AGFAYSSPGDFEAVRRIALEVEGPVVCSLARARPEDITRASEALRGSPRVRIHTFLST
                     SDIHLKHQFRMTREEAKKRAVEMVQLARTYVDDVEFSPMDASRSDPAYLCEVIEAVIA
                     AGAGTINIPDTVGYAVPQEFGGLIKRIKDSVPNSGQAVISVHCHNDLGLAVANSLAAV
                     VAGAGQVECTINGIGERAGNTSLEEIVMGLRTRRDWYGADTKVVTEEISKTSRLVSKI
                     TGMVIQPNKAIVGANAFAHTSGIHQDGLLKDKTTYEIMRPESVGLEQVKLVMGKLSGR
                     HAFRQRLEDLGYKLTEEEVNHAFERFKRLADQKKEIYEEDLEVIVSEEVAKMSERVVL
                     KSFQVESGTNKTPTATVELEIDGKLVSHSGTGDGPVDAVYRTIAAMTQTKSKLLMFGV
                     NAITGGTDAQGEVSVRVEEDGRTVSGHGADTDIITAAARAYLNALNKLAYFAAKQAEG
                     IQKVSLI"
     gene            7703..8065
                     /locus_tag="PKK81_02355"
     CDS             7703..8065
                     /locus_tag="PKK81_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV31739.1"
                     /translation="MLNTHLQRCPEFLAGDHTRLRELLHPAKASLALGYSLAHGLLAP
                     GQQSLWHRLKSSEVYYFIAGRGVMKVEDESVAVEAGSVIYVPPGAKQSLVNNGTAPIE
                     FLCLVDPAWTAEDEAVGG"
     gene            8084..9160
                     /gene="leuB"
                     /locus_tag="PKK81_02360"
     CDS             8084..9160
                     /gene="leuB"
                     /locus_tag="PKK81_02360"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNV31740.1"
                     /translation="MNAKIAVLAGDGVGREIVPEAVKVLKVVAERYGHTFEFISGDVG
                     GHAIDKVGVPLPAETLAIAKHSDAVLLGAVGGPKWEGLEYSLRPERALLGLREQLGLY
                     ANLRPAKLYPMLADASTLRRDVIEGIDMLVIRELTGGIYFGKPKGIEKLPGGGERGFN
                     TEVYTTEEIRRIAVVAFDAARKRRKKVTSVDKANVLESSELWRRVVTDVHKGYPDVAL
                     SHIYVDNCAMQLVRNPRQFDVLLCNNIFGDILSDEAAMLTGSIGMLPSASVGAKVGLF
                     EPIHGSAPDIAGKNIANPIATIASASMMLSYAFQLDKEAAAIEQAIVKTLELGFRTKD
                     IHAEGMQLVGTVEMGDAIVRNLPS"
     gene            9164..10378
                     /locus_tag="PKK81_02365"
     CDS             9164..10378
                     /locus_tag="PKK81_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNV31741.1"
                     /translation="MTTRTTQSGIIETLAGNGEPGYAGDGGPAGAASLNEPKGLCVDR
                     EGNLYIADSENHVVRRVDRVTGIITTVAGVCPSGATGPAVPEPPVAAQAEEDEDPFAD
                     HSGDSTKAYTQVTDLSGTVRYVTGGRLTVEHDSGDGGPARRARLNFPSAVAVDRAGNL
                     YIADTMNHRVRKVDAATGIITNVAGTGQARYSGDDGPAVSAAINEPTGLAVSDEALYI
                     ADQSNNRVRRVDLATGVITTVAGDGTAAYSGDQVSAVQASLAGPSGVALGGDGVVYVA
                     DTFNSRIRSVDPATGQIATVAGDGGTYRYQGPAEPSSPSLSRPAGIAVGSDGNVYMTD
                     SDSHLIRVWNGRSKTISRLSGTGVAQFGGDGGDALAGSLSYPFGVAVDASGTVYVADT
                     FNHRIRVLTATA"
     gene            10389..11408
                     /locus_tag="PKK81_02370"
     CDS             10389..11408
                     /locus_tag="PKK81_02370"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNV31742.1"
                     /translation="MLKKKQAYTVAILGATGAVGKESLEILEERNFPIETLRLFSSKR
                     SAGDVLSCQGKEYKVEELTEASSFAGVDIAFVSATDTISRDYGARLGAAGVVVIDDSA
                     VFRMDPDVPLVVPEVNAAALRSMKRGIVAIPNCTTTPLVMALKPLRDIAGIKRVVVTT
                     FQSVSGTGSAAMDELVDQTKALMAFQDVKVQVYPYQIAFNLLPQVGSFGDGGDCSEEV
                     KIVQETRKILEMPSLRVTATTVRVPVLRCHSEAINVELERPLKANDARAALAEMPGVI
                     VYDDPVKKLYPMPFDVSGKDDVYVGRVRVDESITNGLNLWVVSDNLRKGAALNAVQIA
                     ECLIQ"
     gene            11412..11666
                     /locus_tag="PKK81_02375"
     CDS             11412..11666
                     /locus_tag="PKK81_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNV31743.1"
                     /translation="MAAWTGIGRSLIVAGLVLVAVGLLLTLAEKWPGLGSAFAWIGKL
                     PGDFSVTRERFSLYVPIATSLVFSILLSLVFYFLSWLFRR"
     gene            11648..12859
                     /locus_tag="PKK81_02380"
     CDS             11648..12859
                     /locus_tag="PKK81_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNV31744.1"
                     /translation="MALSPLTRHQSSSLAAAGGMLLLALFCVPAVFAESIRVLLAQEA
                     PLVEVRSEGALALVTETGDTKILQAPIHLTARGDGVHVDGRRSMGGQVLIRPLRNNLS
                     LVIGKEGGATAGAPLALSGVVRLSRKGHGLSVVNQVDLEEYVKGVVPSEVSSAWHPEM
                     LKVQAVAARTYALYNKMLSAARDYDVVATIQDQVYRGRTGVDRRVEDAVESTRGIVVT
                     HQQAPIYAAFSSTAAGPTEDAVNVWANKDLPYLKGVECPFDLESPYYQWRASVKIDQL
                     EHNLRHQGFSVGTIATITPIAYSRAGRVSRLRILHSGGETVLRGEDLRKAVGYTVIPS
                     TQFEVESIGAEVVFAGYGAGHAVGLCQWGAKELAELGYSYSSILQYYYPGTELHNAAL
                     SQLMMPVIPTP"
     gene            12859..13899
                     /gene="queA"
                     /locus_tag="PKK81_02385"
     CDS             12859..13899
                     /gene="queA"
                     /locus_tag="PKK81_02385"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNV31745.1"
                     /translation="MLLSEFDFPFDPVLVADHPVIPRDRARLLVLDRQGGERVHRQVA
                     DLPSLLQSGDLVVVNNTKVMPARVPAQVRSNGKLLDLLFVEDLGQGLWEVLIKGRFRP
                     GAVIEFPGGGTGEIVERSRLRTTVQVSGVASVYELMQATGTMPLPPYIKREPSADDQE
                     WYQTMFAQHEGAIAAPTAGLHFTRELVQALAGKGIELAQVTLHVGPGTFRSVKTERIE
                     DHRMLAERIEVSAQTVAQIRLTRERGNRVVAVGTTVVRALETAARGGRGIEPFQGPAE
                     LFITPGFEFQVIDAMVTNFHLPRTTLLMLVSAFVGVQPLRAAYEEAVAQRYRFYSYGD
                     AMLIGSGWATVT"
     gene            complement(13892..14590)
                     /locus_tag="PKK81_02390"
     CDS             complement(13892..14590)
                     /locus_tag="PKK81_02390"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNV31746.1"
                     /translation="MIKLLREAAHDYPWFLKSIMGLLALAFVITMGWWGFGQEGGNVV
                     ASVGDQVVPLDEYRRAYENTYRFYKDKGQNDIKDEFLKQFVLEQLIDNRMWLHVAKEM
                     GLTVSDEDLRKAIMQRTEFQKNGNFDPEAYRRLLSANRLTPASFEAMEAKDILTNKAR
                     LVIMDAVALTPAEYTEAQTLAAREGDADPAKAAAAKERVFQSLLFQKQQRALMAYAES
                     MKSKVPVKIHKELM"
     gene            14664..15209
                     /locus_tag="PKK81_02395"
     CDS             14664..15209
                     /locus_tag="PKK81_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNV31747.1"
                     /translation="MNGARGKIATLFVQVKEICYSVEELERSPVVEEAIGHSPLELGV
                     YPKAQVLNRFIAKMIDLLIVAAVSKLVPPIGVLAGLAYLLLADGFGGGRSVGKRLIGL
                     QTIVPRTRDPAGFRESIIRNLPCGLAQLAFEIPYVGWIGWGAVLSLEGLLVIGNEQGR
                     RLGDEIAKTQVLESGQLDVSD"
     gene            15267..16307
                     /locus_tag="PKK81_02400"
     CDS             15267..16307
                     /locus_tag="PKK81_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNV31748.1"
                     /translation="MSDMFGWFSNDLAIDLGTATTLVYVQGKGIVLNEPSVVAVEKKT
                     ERVMAVGADAKRMLGRTPGNILAVRPMKEGVIADFEKAEAMLSHFIQKAHNRTAFVRP
                     RIIIGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMVVDI
                     GGGTTDIAVISLGGIVYSESVKVAGDRMDDAIMNYIKKKYNLLIGEHMAERIKFEIGS
                     AYPFEERKTMMIKGRDLISGIPRTLVVDDAEVREALQEPIGTIVNAIKVALENTPPEL
                     AGDIIDRGIVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELDLLR
                     KVSVMSQCSTSR"
     gene            16428..17207
                     /gene="mreC"
                     /locus_tag="PKK81_02405"
     CDS             16428..17207
                     /gene="mreC"
                     /locus_tag="PKK81_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNV31749.1"
                     /translation="MLQYVGGPLGQILSVPLAAFSSVDHGISETWNAYLALQSVQEEN
                     RQLHRDLELLKGQNNQLRESVAATRRYETLLNFKQQSPSQTLAAQVIGRDATNWYGGM
                     ILNKGESDGVRVEMGVVTPAGVVGRIVKTNSTSSVVLLVTDPNNAIAGVVQRTRDEGI
                     VEGTSHGRARLKYIPLLSRVQAGDRVVTSGLTGTFPRGLAIGGLTQVEKSEGDLFQSA
                     EIEPEVDLSKLDEVLIITAPYEDADSAQKLLQEIRGDRKKP"
     gene            17204..17704
                     /locus_tag="PKK81_02410"
     CDS             17204..17704
                     /locus_tag="PKK81_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31750.1"
                     /translation="MKFLLYLLLALVVVPFQTTLLHYFSIFGVRPDLGLVAACLVGFL
                     GGELDGLILGLLLGCFQDMLSAGDLWINVVTKGGGGFLAGLAGRHMAHITPAVLTAGL
                     AIISCLSSAVFLYSMNPAGIDEVWMGVRSTVLVQAGFDALVGTGLYLLFRQRWSDDRM
                     VTEGAL"
     gene            17750..19621
                     /gene="mrdA"
                     /locus_tag="PKK81_02415"
     CDS             17750..19621
                     /gene="mrdA"
                     /locus_tag="PKK81_02415"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNV31751.1"
                     /translation="MASIGFNDSDLLDLQRRLVILRVGLLLVVALLALRLWHLQIREG
                     PYYRDLSENNRTRSVVLEPARGLIFDRNGVLLANNVPSFALYVTLEDVKDRPALVAQL
                     ASLLNLEPEVIQKKLSTGKGSKLQPRKVKDRLTLREATLIESHRLDLPGVMIQVESQR
                     NYPGGPVAAHLLGYVGEVSADQLEKADFADLHQGSVVGQYGVEKWFDRQVRGRAGQKS
                     VEVDALGHEKRAVVSDKPVAGDDLYLTIDARLQKTAEDLLGEESGAIVALDPTNGDIL
                     AMASRPGFDPNMLSKELTNKQWVEIVQNERRPLNNRATQGQYPPGSTFKVVMAAAALE
                     SNTVTPSTMVRCNGGYQFGNRLFHDWKQGGHGSVDMNEALIHSCDVYFYTVGQRMGID
                     TIAEYAKQFGLGQETGVELPSERVGIVPSTAWKQKARNQPWYPGETISAAIGQGYVTV
                     TPLQMASVIGTVANDGLSIKPRLVQAVMNRATGERAEFPPTVRGKVAVKPATIALIKA
                     ALADVVTKGTATRAKSSLVTIGGKTGTAQTAALRTGPEKDIPKRLRDHAWFVAFAPVA
                     APRIAVAVLAEHMGHGGSAAAPLAKDVIEAYVKAYPEVLNGVPAGGRTKSADARPKT"
     gene            19621..20739
                     /gene="rodA"
                     /locus_tag="PKK81_02420"
     CDS             19621..20739
                     /gene="rodA"
                     /locus_tag="PKK81_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNV31752.1"
                     /translation="MIDRVIDSRGLDSFDLRFMGLIAVILSVGVLSIYSVTHSQDTAF
                     PFYLKQLVWILLGTIAFLVMYLSDYHKIARLAYPTYAVILIMLAVVLVMGKSSRGAQR
                     WIPIGPFAFQPSEFAKLVLVLVLANYYSRVSRAGWLHRVVLPGLIVLPGLLLILKQPD
                     LGSGLSFLAVYAAMLLMVGVRSKTLGVILLLSVMLFPFVWEMVWASLHDYQRERVMAF
                     VDPEYDPGGKGYHALQSRIAIGSGELSGKGLYGGTQSQLKFLPEGHTDFVFAVYAEEW
                     GFVGVLVLLALFIALIWVSLEIAARAKDTLGALLAAGIVAMLCFCVVVNIGMTAGMFP
                     IVGIPLPLVSYGGSATIMTMASLGLLLNVKRRRLTLFY"
     gene            20885..22396
                     /locus_tag="PKK81_02425"
     CDS             20885..22396
                     /locus_tag="PKK81_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNV31753.1"
                     /translation="MGVEIAISIAREETRVAVLDNGVVTDLYGDRAKDQDFVGNIYKG
                     RVAKVLPGMQAAFIDIGLEKAAFMHVSDLSMDVEPGDTLVEGDDDDNKDSEVPKPKRQ
                     SSKPIEELLSEGQEVMVQISKGPIGTKGSRVTTYVSLPGRYLVFMPTVEHIGVSRRIP
                     RDEERARLKEIMKRARRPGFGYIVRTVSEGVKEEELRSDVEFLHVLWQDVLTKRDQQP
                     APSLLHSDLSLSFRVVRDLFGRRVDRLLIDSREEYNAIKGFVQRFSPEQTSRIHFYDK
                     EESLFDYLGVEQEIARAMSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETI
                     LRNNLEAAREIAYQVKLRGIGGIIIVDFIDMEREKNRDKVYHALVDAMSSDKARTRIS
                     RVSDLGLIEISRERVREDLLRSLSEPCHYCDGRGYTKSPMSVAYEIFREVRRLGHEIE
                     QQRVVVGAHPAVALLLQEQEQPGVEELERRYSAKILVTPDDRLHLEQFDLVVI"
     gene            22516..23652
                     /gene="dprA"
                     /locus_tag="PKK81_02430"
     CDS             22516..23652
                     /gene="dprA"
                     /locus_tag="PKK81_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNV31754.1"
                     /translation="MSHRSLRPWLVLRAIPGVGDAILLKLVHAFGAPDAVLSAPQSAL
                     EEIGCRPPLVEAIRRGPAPDAIRELDKELEQLQRRKVTVLTYLDAQYPAPLRTIPDPP
                     PLLYVQGSLLETDRHAVAIVGTRKVSAAGRIVAEELARDLAKMGFTIVSGLARGVDAA
                     AHRGALAGKGRTLAVMGCGLDRTYPADHRQLRETIEQEGAVLSELPLGAAPHSYHFPR
                     RNRIISGLSLGVVVTEAAIESGSLITARLAGEQGREVFAVPGFVKAEQSRGPNSLIKD
                     GARLVESAQDILEELLPQLDPAFRERLAGGAGTVAQPKVQFSPDETLVYDVLSVLPQS
                     VDDVIRRSGLPAAQVAAILLSLELNNSIRQLPGNEYVRVTSGMH"
     gene            23735..26074
                     /gene="topA"
                     /locus_tag="PKK81_02435"
     CDS             23735..26074
                     /gene="topA"
                     /locus_tag="PKK81_02435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNV31755.1"
                     /translation="MAKSLIIVESPTKARTITKYLGRGYSVMASVGHVKDLPTSKLGV
                     DLENDFEPQYVTIKGKSKVLADIKKKAQEVDTVFLAPDPDREGEAIAWHIAQELHGKG
                     KKKDGKVYRVLFNEITESAIKRALKSPGEIDMKLVQAQQARRVLDRIVGYQGSQLLWK
                     KVRRGLSMGRVQSVAVRLICDREKEREAFRAEEYWSIVALLAGDNPPAFEAKLHSVNG
                     QDADIGTGERAQQILDAVQGKPFVVQSIERKEKKRNPVAPFITSRLQQEAARKLHFSP
                     KKTMTLAQQLYEGIEIGAEGPTGLITYMRTDSPRISQEATEDARVVIRERFGVEYLPA
                     TPNVYKTQKAAQEAHEAIRPTSAARDPESIRQYLEQDQYNLYKLIWNRFIASQMVPAI
                     MDVTRVDSSPVGTPDRYVFRTTGTVVKFAGHTAVYLEGTDKELPSRKPKNEQEAEDES
                     DRQLPALREGESLHLVAQEAQTLPGLVSKQHFTQPPPRYNEALLIRELEEKGIGRPST
                     YASIISTIQDRKYVEKVEGRFLPTETGRTVNDFLLKGFPDLLDTEFTSHMEEQLDEVE
                     EGTKPWVSAVRDFYTPFVREMELAQGIPGPKDIVEPPTNLPCEKCGRMLEIKWGRNGK
                     FLACPAYKDDPPCKNTQNFEKLPDGTIKIVPKLEETTDEKCEKCSSPMVVKSGRFGKF
                     LACSAYPECKTTKAIALGVKCPQPGCGGDLVQKRTKKGRNFYSCSRYPQCEYALWDRP
                     INKPCPKCQAPFLIEKVSKQAGRSVQCRNEEECGYREAG"
     gene            26395..26871
                     /gene="bfr"
                     /locus_tag="PKK81_02440"
     CDS             26395..26871
                     /gene="bfr"
                     /locus_tag="PKK81_02440"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNV31756.1"
                     /translation="MKAKQGVIEILNKVLTADLTAINQYFVHAKMCSNWGYERLHHKV
                     RERSIDEMKDADELISHILYLEGVPNVQRMNTVHVGETVPEQLKLDLKAEQEMLTLLS
                     DGVVHCTKVGDFTTRHMFEDMAKDVDEHIDWIETQIETIKQVGLENYLAEQIHKES"
     gene            26907..27386
                     /gene="bfr"
                     /locus_tag="PKK81_02445"
     CDS             26907..27386
                     /gene="bfr"
                     /locus_tag="PKK81_02445"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNV31757.1"
                     /translation="MKAKEGVLDILNKVLVSELTAVHQYLLHAALCKHWGYGRLHEHF
                     NHLAQEEVSHSSGLIDHILYLGGAPEMDHLDAVAAGKKVSDLLEADLAFERDDADVLR
                     DGIAHCTKVADFTTRRRLEEMIVDTEEHIDWFETQLCTIKQVGLDRYLAEQIHSEKS"
     gene            complement(27639..28202)
                     /locus_tag="PKK81_02450"
     CDS             complement(27639..28202)
                     /locus_tag="PKK81_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNV31758.1"
                     /translation="MMMNNAPDIEIPTDRLRTLSSELRAQSPHWADRLEQATTEQELR
                     NLVCDLFNLSHALPSHRESANETTTLAGRITRFINENLHRGLTLKVLANFLGYSEKYC
                     SDLFYRIMGESFSGYMRRHRVERAQSLLTTTAQTLAEVATAVGFSDQFAFSHFFKRAT
                     GHSPREIRSRQAHSRHRLTVHAMQKAL"
     gene            complement(28265..29020)
                     /locus_tag="PKK81_02455"
     CDS             complement(28265..29020)
                     /locus_tag="PKK81_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNV31759.1"
                     /translation="MHPSISPINATTQLIQEITRQLDALDSLQLSKESLHAVRLLQLR
                     RLTRRIETLIPGHFGHGERTAHYARLLGKAISLTDDQHIELHYAALLHDIGLLTLPGR
                     LLDETAAHSLDDYALIQSHPREGAALLSPHRFLSEAARLIAHHHERWDGAGYPYGLRG
                     GYIPLAARILAIADVFDSIANRSTSLHRALRTLQASAGSQFDPILTATFCARVRDQDQ
                     LPPLPFGDASVTALTVEPTLDQPSHQHMPFSMF"
     gene            complement(29125..29643)
                     /locus_tag="PKK81_02460"
     CDS             complement(29125..29643)
                     /locus_tag="PKK81_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31760.1"
                     /translation="MRNFNARFRLRDSDRHIRLLYTQFLFLMLVGFLFSFFWAHSMTS
                     LSPQGIADHYRGSDATFGEPMSFRELAEVTHFHLFTMPVVFMILVHVLYLTQASHATQ
                     VWLTWLSFGGVALDLLSPWLISYISPIFVLTMLAGDTLMMGSFLAMMAIPLYEMWILK
                     QPLMAGKRGEES"
     gene            complement(29667..30353)
                     /locus_tag="PKK81_02465"
     CDS             complement(29667..30353)
                     /locus_tag="PKK81_02465"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNV31761.1"
                     /translation="MFSTLSAYRTLTAVVLLLTSSLPALATEKVWDNELRRFLKDDDF
                     KYEEFMTEEEAVKTILPKSQRVRKELIRLTQDRKELIEQRIGWKFPEDSFDLYIGETG
                     DKVDGYAMIHNTIGKYKPMTYMVGVDPKGNCTDVELLVFRDAKGSEVGKKRFNSQYDG
                     KTVTDPIRINKDIINISGATMSVRSMNAGVKRVLVLVDEFYLKPAGLGSDTVAVQKAE
                     RGLLGTLFGN"
     gene            complement(30338..30799)
                     /locus_tag="PKK81_02470"
     CDS             complement(30338..30799)
                     /locus_tag="PKK81_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31762.1"
                     /translation="MTQHYRRLSVLLIPALLTACASLGGMQERFAVCSYDHAWEAALD
                     AVKDRAIARQDKAAGLIDTAWLEIPMPGRTFGALQRDLGDSKDRSRISLAVKRLDDVT
                     KIGFIEERQRWAFRGGSRLFGWTDTESSAQVMTDVQNRLDTKLKEHGCSVH"
     gene            complement(30860..>31216)
                     /locus_tag="PKK81_02475"
     CDS             complement(30860..>31216)
                     /locus_tag="PKK81_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31763.1"
                     /translation="GMQGYYVQGNYHFMPEFLKKWAPSHFTDASTFTAVVRWEQVNTD
                     TDDRTKQNALGGQRKLERLTLGLNFRPIEDTVIKFDWQFNTQRDAKGLVAAGDLGTAG
                     SPMNGNGFLIQAATYF"
BASE COUNT         6428 a         8128 c         9961 g         6699 t
ORIGIN      
        1 gtgatggcga catcaacggc acggtaagcg tcgtccggag caagaccaaa tagggggacg
       61 cctgtgggct cgcgagagct cggcagaaag gccggcccgg ccgaagttcc cgggtaggtt
      121 gcttgaggct cgaggtaact cgagtcccag agaaatgatc acccccgtgt gagcgcaagc
      181 ttcacgcggg acagaatccg gcttataggg tttctccaac gcgctttttt aatcgcttca
      241 cgcagctctc cggcggtcac cttgccggag aacgtttccc ggtcattgat caacagcacc
      301 ggcacgcggt cgctgtactg tttgcggagg cgctcgtcac tgtcgatatc gactgtgatg
      361 agctcaaagg gaaggtcttg ttgcacttgt ccgaccaccc gtgtgaccac tcgacaaaga
      421 tggcactcgt ggcgggacat gatggtgacg cgtagaggat tcataggcag cacgatcaat
      481 gggttggctg actcccctat atcacggggc atgagccggc accagtctcc ccggcgaaga
      541 attcgtgcat cggctctctt gacctcactt gagagctggt gatacgatcc gccatcttac
      601 ccgttgattt tccagaaaga ttgtatcagt ctgtgccgcg tgtgtgacgc agacagtcga
      661 gtgttctcag ttaaggcatc gcgtccttcg tcacgatgtg cgaaggggag ggtgatatga
      721 agcttacagg gtcggaaatc ctgatcgaat gcttaaaagc cgaaggcgtg aaaacaattt
      781 tcgccttgcc gggcggtgtg gtgttgaaga ttttcgacat gctgcaccag caaaaagata
      841 tcgaagtcgt cttgacccgg cacgaacagg gcgcggggca tatggccgaa ggctatgcca
      901 aggctaccgg caaggccggt gtgtgtttgg tgacgtccgg gcctggtatg accaacgtga
      961 ttacggctct ggccgatgcc tatatggatt ccgtgccgtt ggtgtgtatc agcgggcagg
     1021 tctcgacgag tttgatcggc aacgacgcct ttcaggaagc cgacaacatc ggccttagcc
     1081 ggccttgcac aaaatacaat ttcctcgtga aggacgtgaa cgacctggcg acgaccatca
     1141 aggaagcgtt ctatatcgcc acgaccggcc gtccaggacc ggtcttggtg gatattccta
     1201 aagacgtgtc catggccaag acagagttca cctatcccag ctcggtctca atccgcggct
     1261 acaatccgac ctacgaggga aataagtggc agattaagca ggctgccgaa gccatcatga
     1321 aggcgaagaa gcccattctg tatgtcggtg ggggcgtcgt cttttcgcag gcatccaaag
     1381 agttgctcga attggcggag atgactcaga tccccgtgga tatgaccttg atgggcctcg
     1441 gggcgttccc gggcgaacat ccgcagtcca tgggcatgat gggcatgcac ggcacctact
     1501 gcgccaacat ggctgtccat tattccgatc tggtcattgc ggtgggggcc cgctttgacg
     1561 atcgtgtgac ggggaaggtg tcggagttct gcccgttcgc gaaggtcatt cacatcgata
     1621 tcgatccgac ctctatccgg aagaatatcc atgtcgatat tcccatcgtg ggcgattgca
     1681 aggccgtctt acgggagctg aatcaaattc tccgtgccac cgtgaacggg aaccaaaagg
     1741 atctgcgcaa gccctggtgg gatcaaatcc gcgaatggca gcaggcgcat ccgttggcct
     1801 accagcagga gcccgatggg gcgattaaac cgcaacatgt cgtcaagcgg ttgtatgaac
     1861 tgacgaagga tcgtgatccg atcgtggcga ccgatgtggg ccagcaccag atgtggacgg
     1921 cgcagtactt caagcttgcc cgaccgaatc gctggttgac ctcgggcggt ttgggtacca
     1981 tggggttcgg gtttccggct gctatggggg cgcaggccgc gttcagagat cgattggtgc
     2041 tctgtgtggc cggcgacggc agcatacaaa tgaatatgca ggaaatggcc gccgcggtgg
     2101 tgtcgaagct gccggtcaag gtgattattc tgaacaatcg ttttcacggc atggttcggc
     2161 agtggcagga cctgttttac gaaggacggt atgcgtcgag ctacctcgac accacgccgg
     2221 atttcgtgaa gctggccgaa gcctatgggg cggtcggttt gcgcgcaagc aaggtcgggg
     2281 atctggatac cgtgttgaag gaagccatcg cgaccgacaa gcccgtcgtc gtcgatgtgc
     2341 cgacctatcc ctatgagaat tgttatccga tgattccggc gggcgggtgc aatcacgaga
     2401 tgattctcga agatccgcct gaactgaagc gaagaatggc cggcgcgccc agcaccgggt
     2461 ccgacgaaga taaggacacg atcttaaccg cttaaatcac gctgtcagcc gacagctatc
     2521 aggatagagt tttccgatgg aacatatcat ttcggtcacg gtagaaaata agttcggcgt
     2581 gttatcccgc gtggccgggt tgttcagcgg ccgcggcttc aatatcgaaa gtttgtcggt
     2641 cgcccccacg ctggatccct cgatgtcgca gatgaccatc gtcacgtcgg gggacgaacg
     2701 gatcgtggaa caaatcgtca agcagctgaa taagctgatc gatgtcatca aggtcgtgga
     2761 tctcaatgag agtgaattcg tttcgcggga gacggcgctc atcaaggttc ataccaaggc
     2821 agaagaccga gccgaggcgc tccggatcgc agatattttc cgggccaacg tgattgactc
     2881 gacgccctcg acctacacca tcgaggtgac gggtgatcca aagaagctcg aggccatcat
     2941 taatctgctc cagccgctcg gcatcaagga actgattcga acggggcgag tggcgattgc
     3001 gcgtgaagcg atccggccgg ccctcagcca acccaaaaag atcgcccggg aataattcgc
     3061 tcatcccctg tgtgcaactg tcgtttccac ccatgtgccc tatgaacctt caggagtgat
     3121 gtatgaagat ctattacgac aaagatgccg atttacagct cattcgcgga aagaaagtgg
     3181 ccgtgatcgg ctatggcagc cagggccatg cccattcgct caatctcaag gaaagcggcg
     3241 ccaatgtcgt cgtgggtttg cgtgaaggca gttcctggaa gaaggcggaa gcgagcggac
     3301 tgaaggtcat gccggtggcc gatgccgtca aagcctccga tgtcatcatg attctggctc
     3361 ccgatgaggc acaggcggcc atttaccgcc aggagatcgc tccgaatctg aagcccgggt
     3421 cctatttggc cttcggtcac gggttcaata ttcacttcgg gcagattgtg ccgcccgcgg
     3481 acatcaacgt cttcatggtg gcgcccaagg gaccggggca cctggtgcgg tcggaatata
     3541 caaaaggcag cggcgtccct tgtttgctgg cattgcatca agatcccagc ggcaacacgc
     3601 gccaggtggg tttggcctac gccagcgcta tcggcggcgg acgggccggc atcattgaaa
     3661 cgaacttccg tgaagaaacc gaaacggatt tgttcggcga gcaggcggtg ttgtgcggag
     3721 ggttgacctc cctgatccag gccgggttcg agacgctcgt cgaggccggg tattcgcctg
     3781 agatggccta tttcgagtgt ctgcatgaag tgaaattgat cgtggacctg atctatcagg
     3841 gcggcatcgc caacatgcgc tattcgatca gcaccacggc aaagtatggc gacatcaccc
     3901 gcggaccgcg cgtggtgacg gatcagacca agcaggaaat gaagaagatt ctcggcgaga
     3961 tccagagcgg tcagttcgcg aaagaatggg tgttggagaa ccaggcgaac cggccggtct
     4021 acaacgccct gctaaaaaag ggcgagggcc atccgattga agaagtcggc gcccgacttc
     4081 gctcgatgat gccctggttg aaaaaagatc agctcgtcga caagaacaaa aactaaccct
     4141 gcgaccgggc tcttcgtgat agcatcatta agaaggcctg gaggtctatg gctgatcgag
     4201 ccgtcgggat accgatcgta aaagaaggct tgccgtttgt cggtggtctt gtgggcgcga
     4261 cgctgctgtt cggagcggcc ggttggatca ttccggccgt gattgcgggc gggttcacgc
     4321 tgtttacggc gtggttcttt cggaacccga gtcggaccat tccgcagcag ccgaatgtgg
     4381 tggtgtcgtc gggggatggc aaagtcattg cgattgagga agagttcgag ccgcgctacc
     4441 tgaaggaaaa gagcatccgt gtgacggtgt tcttgaacgt cttcgatgtg catgtgaacc
     4501 ggatgccctg cgccgggacg gtggagggtg tgagttacca gcccggccag tttctcgtgg
     4561 cgagcaagcc ggaggcgacg ctcagaaacg aacagaacgc cgtgatgctg aagacggagt
     4621 caggggcgaa agtgctatgt gtgcaggttg ccgggctgat tgcccgccgg atcgtgtgct
     4681 gggtcgggca gggtgatcgt gtggaacggg gagagcggtt tgggctgata cggtttgggt
     4741 cgcggatgga taccttcctt ccgctcggct cgaagatttg tgtctccttg ggtgaccgcg
     4801 tcaaaggggg cgaaaccatc gtgggggaac tccgatgaaa tcaccggcca tgcgagctcc
     4861 gtttgcgaag ggtaatcgaa agcgacaggc catgtatctg atcccgaacc tctgcacgac
     4921 gggaaatctg ttctgcggcg tgttcgcgat cctgtcggtg tttaacgggc agcatctggt
     4981 ggcagccatc gccattctgg tcggcatgat tttcgacatg ttggacggga agttggcccg
     5041 actgacgaac agtaccggcc agttcggcat cgagtatgat tctctttccg acgtggtgtc
     5101 gttcggggtc gcacctggcg tgctgattta ttcctatgcg ctgagcggcc agggcatgtt
     5161 cggggtcgca gtgatgtttg cctatgtggc catgggggcg gttcgcctgg cccggttcaa
     5221 tgcgacggtg agcacctccg acagcaaata cttcacgggc ttggctattc ctgccgcagc
     5281 cggtgtcatt gcgtcgttgg tgatcttcga tctgcacatc acccagatgg gctcggaagt
     5341 gaagccgctg ttgatcctcg tgatcacgtt cgcgctggcg ttcttgatgg tcagcacgat
     5401 caagtatcgc agcttcaagg acatgaagtt ccggcgtggg gatcatttca cctatctggt
     5461 gtgggggatt cttgcgctga tgctgattgt cgcctggccg caggcgatgt tgttcgtgac
     5521 gtttaccggg tacgccgtgt cgggtccatt ggcgcgtctg tggacgatgg tgagcaagag
     5581 tgcagggaag ccggtggcga aaacggacac gccggtactc gattcgagag agtaacaaca
     5641 gcgacgacga ggagtagtag gcagcgctga ccgaactgag agagctggtg gatggtgcga
     5701 accagccggt gatctgccga actcgcctct gagctggact tgtgaagccc cccggggttg
     5761 taatgggttc cgaatgatcc gcgtcacgga ttgaatgaag ggcgttcgga tgaaggacat
     5821 acttcatccc tcgctgaatc agggtggtac cacgaagctc gcaaggcctt cgtccctgtc
     5881 ccggggcgga ggcttttttt attggcagca tgctgaaacg gccttccaac tttgttctcg
     5941 gctcgaaaac atcctcaacg taccccagag ggtacgcctc cggtgttttc tcgcctgcgg
     6001 ccgcgttgga agatccgttt gagcaagctg cgggacagga ggagggtaag ccatgaccag
     6061 gatgatcaga atattcgata cgacgttgcg ggatggtgag cagtcaccgg gggcgagcat
     6121 gaacgtcgaa gagaaactta tgatcgccaa gcagctggcg cgcctcggcg tggatatcat
     6181 cgaagccggc ttcgcctaca gttcacccgg agatttcgaa gcggtcagac ggatcgcctt
     6241 ggaagtcgaa ggcccggtgg tgtgcagcct ggcgcgggcc aggccggaag acatcacgag
     6301 ggcgtcggaa gcgttgaggg gctcgccccg tgtgcgaatc catacgtttc tctcgacgtc
     6361 ggacatccac ctcaagcacc agttccggat gacccgcgag gaagcgaaga agcgcgcggt
     6421 ggagatggtg cagttggctc gcacctatgt ggatgacgtc gaattttcac cgatggacgc
     6481 gagccggtcc gatccggcgt atctgtgcga agtgatcgag gcggtcattg cggccggggc
     6541 gggaacgatc aatattcccg acacggtggg gtatgccgtg cctcaagagt tcggcggcct
     6601 gatcaagcgg atcaaagaca gtgtcccgaa tagcgggcaa gccgtcatct ccgtccattg
     6661 ccacaacgat ttggggctgg ccgtcgctaa cagcttggcg gcagtggtgg caggggccgg
     6721 acaggtcgag tgcacgatca acggcattgg cgagcgggcc ggcaacacct cgctggagga
     6781 gattgtgatg ggtctccgga cgcgtagaga ttggtacggg gctgacacca aggtcgtgac
     6841 ggaggagatc tcaaaaacca gccgcctggt gagcaagatt accggtatgg ttatccaacc
     6901 gaacaaggcc attgtcggtg ccaatgcctt cgcccacacg tcggggattc accaggatgg
     6961 gttgctgaaa gataagacga cctacgagat catgcggcct gaatccgtcg gtcttgagca
     7021 ggtcaagctg gtgatgggca agttgtccgg gcgccatgcg tttcgtcagc ggctcgagga
     7081 tttgggctat aagttgacgg aagaagaggt caaccacgcg tttgaacgat tcaagcggct
     7141 ggcggatcaa aagaaagaga tttatgagga agatctcgaa gtcattgtgt ccgaagaagt
     7201 cgcgaagatg tcggaacggg tggtattgaa atcgtttcaa gtcgaaagtg gaaccaataa
     7261 gactcctacg gcgactgtgg aattggaaat cgatgggaaa ctggtctctc acagcggaac
     7321 cggcgacggc ccggtggatg cggtctaccg gacgatcgcc gcaatgaccc agacgaagag
     7381 caagttactg atgttcgggg tcaatgccat taccggcggg acggatgcgc aaggggaggt
     7441 gtcggtgcgg gtcgaagagg acggtcgaac ggtctccggc catggcgccg ataccgatat
     7501 cattacggcg gctgcgcggg cctatctgaa tgcgctcaat aaattggcct attttgctgc
     7561 gaaacaggct gaagggattc agaaggtcag cttgatttga gcccaacctg cagggtagat
     7621 tcctccgtcc gggccgatgc gttcgtatcg gactggacgg agcgacatcg tctgtcattc
     7681 ctactatgga tctcggtgca cgatgctaaa cacgcatctt caacgatgcc cggaattctt
     7741 ggcgggcgat cacaccaggc tgcgggaatt gctgcacccg gcaaaggcat cgctcgcgct
     7801 cggatatagc ctggcgcacg gactgctcgc cccgggtcag cagagcctct ggcatcgctt
     7861 aaaatcttcc gaagtgtatt acttcattgc cggccggggg gtgatgaagg tggaggacga
     7921 atcggttgcg gttgaggcgg gctccgtgat ctacgtgccc ccgggggcca agcaatcgtt
     7981 agtgaacaat ggaacagccc cgattgagtt tctctgcctg gtcgatccag cctggaccgc
     8041 ggaggatgag gccgttggtg gatagtcgag aggagatctg cacgtgaacg cgaaaatcgc
     8101 agtcctggcc ggagatggag tcggccgtga aattgttccg gaagcggtca aggtgttgaa
     8161 ggtcgttgcg gaacgctacg ggcatacgtt tgaatttata tccggcgatg tcggcggcca
     8221 tgcgatcgat aaagtcggcg tgccgcttcc ggctgagacg ctcgccatcg caaagcacag
     8281 cgatgccgtg ttgctcggcg ccgtcggcgg gccaaaatgg gaaggtctgg aatatagcct
     8341 tcgtcctgag cgggccttgc tggggctgcg ggagcaactc ggactctatg ccaacctccg
     8401 accggccaag ctctatccca tgcttgccga cgcctccacg ttacgacgtg acgtgattga
     8461 gggcatcgat atgctcgtga tccgggagct gaccggaggg atttatttcg gcaagccgaa
     8521 ggggattgaa aagctgccgg gcggcggaga gcgtggcttc aatacggagg tctacacgac
     8581 cgaggaaatc cgtcgcattg ccgtggtggc gtttgacgcg gctcgcaagc gccgtaaaaa
     8641 ggtcacctcg gtggataagg ccaatgtgct ggagtcctcg gagttgtggc ggcgggtggt
     8701 cacggacgtt cacaaagggt atcccgacgt ggccttgagt cacatctatg tcgacaattg
     8761 cgcgatgcag ctggtgcgta atccgcggca gttcgatgtg ttgctgtgca acaacatctt
     8821 cggggatatt ctcagcgacg aagccgcgat gctcaccggg tcgatcggca tgttgccgtc
     8881 cgccagtgtc ggcgccaagg tggggttgtt cgagccgatc catggcagtg cgccggatat
     8941 tgccggcaaa aacatcgcca atccgattgc aacgatcgct tctgcctcaa tgatgttgtc
     9001 ctatgcgttt cagctcgaca aggaagcagc ggcgatcgag caggccatcg tcaagacgct
     9061 ggaactcggg ttccgcacca aggatattca tgccgaaggc atgcagctgg tcggcaccgt
     9121 tgagatgggt gatgcgattg tgcgcaacct tccctcgtaa gttatgacga cacgcacgac
     9181 acaatccgga atcattgaga ctctggcagg caacggcgag ccggggtacg ccggtgacgg
     9241 tgggcctgcg ggtgccgcct cactcaacga gccgaaaggc ttgtgcgttg atcgggaggg
     9301 caacctctat attgccgatt ccgaaaacca tgttgtgcgt cgagttgatc gggtcacagg
     9361 catcattacg accgtggccg gagtttgtcc gtcaggagcg accggaccgg cagtgcctga
     9421 accgcctgtg gcggcgcagg ctgaggaaga cgaggatccc tttgccgacc attccggtga
     9481 tagtaccaag gcctacacgc aggtgaccga tctgagtggc accgttcggt atgtgaccgg
     9541 agggcgtttg acggtcgagc acgattcagg cgacggcggc cctgccaggc gagcgcgact
     9601 caatttcccc agtgcagtag ccgtcgatcg cgcaggcaac ctctacatcg ccgacacaat
     9661 gaaccaccgg gtgagaaagg tcgatgcggc gaccgggatc atcaccaacg tggccggcac
     9721 aggtcaggcg cggtattccg gcgatgatgg accggcggtg tcggccgcga tcaatgaacc
     9781 gaccgggttg gcggtcagcg acgaggcgtt gtacatcgcg gatcaaagca ataaccgtgt
     9841 gcgtcgggtc gacctggcga ccggggtcat caccacggtg gccggagatg gaacggcggc
     9901 ctatagcgga gaccaggttt cggccgttca agcgagtctg gccggaccca gcggcgtggc
     9961 gttaggcgga gacggcgtcg tctatgtggc tgatacgttt aacagccgga tacgatctgt
    10021 cgatccggcg acggggcaga ttgcgacggt agcgggagac ggcgggacct atcgatacca
    10081 gggaccggct gagcccagtt cgccgagcct ctccaggcct gctgggattg ccgtcggttc
    10141 tgatggaaac gtgtatatga cggattcaga cagtcacttg attcgggtgt ggaacgggcg
    10201 tagcaagacg atctctcgct tgtccggtac cggtgtcgcg cagttcggcg gcgatggcgg
    10261 cgatgccttg gcaggaagtc tcagttatcc attcggtgta gcggtggacg cgtccgggac
    10321 cgtctatgtt gcggatacat tcaatcaccg catcagggtt ctgaccgcga ctgcgtaagg
    10381 acagcatcat gttgaagaag aaacaggctt atacggtggc gattctcggt gcgaccgggg
    10441 ccgtgggaaa agaaagtcta gagattctgg aagaacgcaa ctttccgatc gagacgttgc
    10501 gcctgttctc ctccaagcgg tcggccggcg acgtgttgtc gtgccagggc aaggagtata
    10561 aggtggagga actgacggag gcctcttcct ttgccggagt cgacatcgcg ttcgtgtccg
    10621 cgaccgatac catcagtcgg gactacgggg cgcggttggg cgcggccggg gtcgtcgtca
    10681 tcgatgacag cgcggttttc cggatggatc ccgatgtccc gctggtcgtc ccggaggtca
    10741 atgcggcagc gttgcgctcg atgaagcgcg ggattgtggc cattccgaac tgcaccacaa
    10801 cccccttggt catggcactg aagccgcttc gcgatattgc aggcatcaag cgggtggtgg
    10861 tgaccacctt tcagtcggta tcgggcaccg ggtcggcggc catggatgaa ctcgtcgatc
    10921 agactaaggc cttgatggct ttccaagatg tgaaggtgca ggtctatccc tatcagatcg
    10981 cgttcaacct tttgccgcag gtcggctcat tcggtgacgg cggtgactgt tcggaagagg
    11041 tcaagatcgt tcaagagacc aggaagattc tggagatgcc gtcgttgcgg gtcacggcga
    11101 ctacggtgcg ggtgccggtg ttgcgctgtc actccgaagc gatcaacgtt gagctggaac
    11161 gtccgttgaa ggcgaacgat gcgcgggctg cgctggcgga gatgcctggt gtgattgtgt
    11221 acgacgatcc cgtcaagaaa ctgtatccga tgccgttcga cgtgtccggt aaggacgacg
    11281 tgtatgtcgg gcgggttcgt gtggacgagt cgatcaccaa tgggttgaac ctctgggtcg
    11341 tgagcgacaa ccttcggaag ggcgccgccc tcaatgccgt gcagatcgcc gaatgtctga
    11401 tacaatgaac gatggcagcg tggactggta tcggtcgatc gttgattgtg gcgggcctgg
    11461 tgttggtcgc cgtcgggctg ctgctcacgc tggcggaaaa atggccaggc cttggctctg
    11521 cgttcgcgtg gatcggtaaa ttaccgggcg acttctcggt cacgcgagag cgattcagtc
    11581 tgtatgtccc gatcgcgacc agtctcgtgt tcagcatcct gctgagcctg gtgttctact
    11641 tcctttcatg gctctttcgc cgctgactcg acatcagtca tccagcctgg ccgcagcggg
    11701 gggcatgttg ctgctcgcgc tgttctgcgt accggccgtc tttgccgaat ccattcgcgt
    11761 cctcttggcc caagaggcgc cgttggtgga agtgcgatca gagggcgctc tcgctcttgt
    11821 gacggagacg ggcgacacga aaatcctgca agcgccgatc catctcacgg cccgaggaga
    11881 cggcgtgcat gtcgatggcc ggcgcagcat gggcgggcag gtcctcatcc gtccgctgcg
    11941 gaacaacctc tcgctggtga tcggcaaaga ggggggcgca accgcgggag ctccattggc
    12001 gctcagcggg gttgtgcgtc tgtcgcgcaa agggcacggg ctctcggttg tcaaccaggt
    12061 cgatttggag gagtatgtga agggggtcgt gccgtcggag gtcagttcgg cctggcaccc
    12121 ggaaatgttg aaggtgcagg ccgtggcggc acggacctat gcgctgtata acaaaatgct
    12181 cagtgcggca cgggactatg atgtggtggc gaccattcag gatcaggtgt atcgaggtcg
    12241 cacaggagta gaccgccgag tggaggatgc ggttgaatcg acgcgaggca ttgtcgtcac
    12301 ccatcaacag gcgccgatct atgccgcgtt ctcatccacc gcggccggcc cgacggaaga
    12361 tgccgtgaat gtctgggcga acaaagacct gccctacctc aagggggtcg aatgcccatt
    12421 cgatctcgaa tcgccctact atcaatggcg ggccagtgtg aagatcgatc aacttgagca
    12481 caatctgcgg catcagggtt tctctgtggg aacgattgcc acgatcactc cgattgccta
    12541 cagccgggcc ggacgggtct cgcgcctgcg gattttgcac tccggcggag agacggtatt
    12601 gcgtggcgag gatcttcgga aggccgtggg gtacaccgtc attcccagca cccagttcga
    12661 ggtggaatcg atcggtgccg aggtggtgtt tgccggctat ggagcaggcc atgccgtggg
    12721 gctctgccaa tggggcgcga aagagttggc cgagctgggc tattcgtaca gcagtattct
    12781 gcagtattac tatcctggaa ccgagttgca caacgcggcg ctctcgcagt tgatgatgcc
    12841 ggtgataccg accccctgat gttactcagc gagttcgatt tcccctttga tccagtactt
    12901 gtagccgatc acccggtgat cccgcgtgat cgcgcgcgtt tgcttgtgct ggatcggcag
    12961 ggaggcgaac gcgtgcaccg gcaggtggct gaccttccat cattgctgca gtcgggggat
    13021 ctcgtggtgg tgaataacac caaggtgatg ccggcccgtg tgcccgcgca ggtccggtcg
    13081 aacggaaaac tcctcgacct gctgtttgtg gaagatctcg ggcaggggct ctgggaggtg
    13141 ctgatcaagg gacgctttcg gccaggcgcg gtgatcgagt ttccgggcgg cggtaccgga
    13201 gagattgtgg agcgcagccg gcttcgaacg accgtacagg tgtcgggtgt cgcaagcgtc
    13261 tacgaactca tgcaggccac gggcacgatg ccgcttcctc cgtatatcaa acgggaaccg
    13321 tcagcggacg atcaggaatg gtaccagacc atgtttgctc aacatgaagg ggctattgcg
    13381 gcgcccaccg ccgggctaca ttttacgcgc gagttggtgc aggccctggc gggaaaggga
    13441 atcgagctgg cacaggtgac gttacacgtc ggccccggca cgtttcgcag tgtgaagacc
    13501 gagcgaatcg aagaccatcg catgctggcc gaacgaatcg aggtgtcggc gcagacggtg
    13561 gcgcagattc gcctgacgcg ggagcggggg aatcgtgtgg tggcagtggg cactacggtc
    13621 gtgcgcgccc tggagacagc ggcgcgaggc gggcgcggga ttgagccctt tcagggaccg
    13681 gccgaactgt ttattacccc ggggtttgaa tttcaggtga tcgacgcgat ggtgacaaac
    13741 tttcatttgc cgcggacgac cctgctgatg ctggtgtccg ccttcgtggg tgtgcaaccg
    13801 ctccgcgccg cctatgaaga ggcagtggcg caacggtatc gattctatag ttatggggat
    13861 gcgatgctga tcgggagtgg ctgggcgacc gttacataag ctctttgtga attttcacgg
    13921 gaactttgct cttcatcgac tccgcgtagg ccatcaacgc ccgttgctgc ttctgaaaca
    13981 ggaggctctg gaaaacccgc tccttggccg cagcagcctt ggccggatcg gcgtcccctt
    14041 cgcgagccgc gagtgtctgt gcttccgtat attcagccgg cgtgagcgcc acagcatcca
    14101 tgatgaccag ccgcgctttg ttggtgagaa tatccttggc ttccatggcc tcgaatgaag
    14161 cgggggtcaa acgatttgcg gacagcaatc gtcggtacgc ctcgggatcg aagttcccgt
    14221 tcttctgaaa ctctgtgcgt tgcatgatcg ctttgcgcaa gtcttcgtcg gagaccgtca
    14281 ggcccatctc tttcgccacg tgcagccaca tgcgattgtc gatgagttgc tccagcacaa
    14341 actgcttcag aaactcatcc ttgatgtcgt tctgtccctt gtctttgtag aagcgatacg
    14401 tattttcata ggcccggcga tattcatcca aggggacgac ctggtctccg acggaggcga
    14461 cgacgttgcc gccttcctgg ccgaagcccc accagcccat cgtaatgacg aacgcgaggg
    14521 cgagcaggcc catgatggat ttgaggaacc atggatagtc gtgagcagct tcgcgtaaca
    14581 gtttgatcat cgagcgcgcc tcttgtcggc aaggtttcgc ggattctact caggcgacga
    14641 tcacaaaggc aagaggaaga cgcatgaacg gcgcgcgcgg caagattgcg actttgtttg
    14701 tgcaggtgaa agagatttgc tatagtgtcg aagaattgga gagatctccg gtggtggagg
    14761 aggcaatcgg gcacagcccg ctcgaactgg gcgtctatcc caaggcccag gtgctgaatc
    14821 gcttcattgc caaaatgatc gatctcctga tcgtcgcggc ggtgagtaag ttggttcctc
    14881 cgatcggtgt cctggccgga cttgcctatc tcttgctggc cgacggattc ggcggcggtc
    14941 gcagtgtcgg gaagcggctc atcgggctcc agaccatcgt gccccgtacc cgggacccgg
    15001 cagggttccg agaatcgatc atccgtaatc tgccttgcgg tcttgcccag ttggcatttg
    15061 aaataccgta tgtcggatgg atcggatggg gcgccgtgct ctcgctggaa gggttgctgg
    15121 tcatcgggaa tgagcagggc cggcgattgg gcgatgagat cgccaaaacc caggtgctgg
    15181 agagcggaca actggatgtg tcggattaag cgggacggtg tggttgatcg caggaatcgt
    15241 ttgaagggag agtcctagtg ggcttcatga gtgacatgtt cgggtggttc tccaacgatc
    15301 tggcgatcga tttggggacg gcgacaactc tcgtgtatgt ccagggaaaa ggcatcgtct
    15361 taaacgagcc ttccgtggtc gccgtggaga aaaagacgga gcgtgtgatg gcggtcggcg
    15421 ccgatgcgaa gcgcatgttg ggacgcaccc cggggaatat ccttgcggta cgtccgatga
    15481 aggaaggcgt catcgccgat ttcgaaaaag ccgaggcgat gctcagccac ttcattcaga
    15541 aggcgcataa ccgaacggcg tttgtgcggc cccgcatcat catcggtgta ccgtcgcgga
    15601 ttacgcaggt agaacaacgg gccgtccgtg attcggccga actggcggga gcccgcgaag
    15661 tgtatttgat cgaggagccg gtcgcggcgg cgatcggtgc agggttgccg attaccgaac
    15721 cgtccgggaa catggtggtg gatatcggcg gtggtacgac ggacatcgcg gtcatctccc
    15781 tgggcggcat cgtgtatagc gagtcggtca aggtggccgg agaccggatg gacgatgcca
    15841 tcatgaacta catcaagaag aagtataatc tcctcatcgg ggagcacatg gccgagcgta
    15901 tcaagttcga gatcggttcg gcgtacccct tcgaggagcg caagactatg atgatcaaag
    15961 gacgcgatct gatttccggt attccccgca cgctggtggt ggatgatgcc gaagtcagag
    16021 aagcgttgca ggaacccatt ggaaccatcg tcaatgccat caaggtggcg cttgaaaaca
    16081 caccgcctga attggccgga gacatcatcg atcgcggtat cgtactgact ggcggcggat
    16141 ccttgctgaa gggaatggat actcggttcc gtgaagaaac aaatcttccg atcattacgg
    16201 tcgatgaccc gttgacctct gtggttctgg gggtcgggaa gattcttgac gagctggacc
    16261 tccttcgtaa ggtatcggtt atgtcgcagt gcagtacctc tcgatgatct ccatcccgag
    16321 tgtggatggc tatatcgcgc tcaacatacg gcaccaggcg tcttgctata gtgctgttcg
    16381 cctgcttgct cgtcgccctt tttcttcttc ctagtcaaag ccagaggttg cttcagtacg
    16441 tcggcggacc gctcggtcag atcctcagtg ttcctctcgc cgcgttttct tctgtcgatc
    16501 acggcatttc agaaacctgg aatgcgtatc ttgccctgca gagtgtccag gaagagaacc
    16561 gccagcttca tcgcgacctc gagttgctca agggacagaa caatcagctg cgcgaatccg
    16621 tcgcggccac gcgacgatac gagacgctgc tgaactttaa gcagcaatcg ccgtcccaga
    16681 ctctggcggc tcaggtgatc ggcagagatg ccacgaattg gtatggcggg atgattctca
    16741 ataagggcga aagcgacggg gttcgtgtgg agatgggggt ggtgaccccc gcaggtgtgg
    16801 tgggacgaat cgtgaagacc aattccacat cctcggtggt gctcctggtg accgatccca
    16861 acaacgcgat tgcgggagtg gtgcaacgga ctcgcgatga aggtatcgtg gagggcacaa
    16921 gccatgggcg ggcgcgactc aaatatatcc ctctgttgtc gcgggttcag gcgggcgacc
    16981 gggtcgtcac ctcaggattg accgggacct ttccgcgcgg tctggcgatc gggggactga
    17041 cccaggttga gaagtcggag ggtgatctgt ttcagtcggc cgaaatcgag ccggaagtgg
    17101 acctttcaaa gctggatgaa gtcctcatta tcacggctcc gtatgaggat gccgactcag
    17161 cgcaaaaact gctgcaggag attcgcgggg atcgaaaaaa gccatgaagt ttctgctgta
    17221 cctgctcctg gcgctggtgg tcgttccctt tcaaaccacg ttgttgcact acttcagcat
    17281 ctttggtgtg cgtcctgacc tcggtctggt ggccgcctgt ctcgtcgggt ttctcggcgg
    17341 cgagttggac ggattgattc tcgggctcct gctggggtgt tttcaggata tgttgtctgc
    17401 gggagatctc tggatcaatg tggtcaccaa gggcggaggc gggtttctgg ccgggttggc
    17461 gggccgccat atggcccata tcactccggc ggtgttgacc gccgggttgg cgatcatttc
    17521 ctgcctgtcc agtgccgtgt ttctctactc gatgaatccg gcgggtatcg atgaggtctg
    17581 gatgggcgtg cgttcgacgg tgctcgtgca ggccgggttc gacgcacttg tcgggactgg
    17641 cttgtatctg ttgtttcgtc agcgttggtc cgacgatcgc atggtgacgg agggggcgtt
    17701 gtaatcgcgt gcgggtgtcc cggcaacaat cggaaaaagt ctggcgacca tggcctcgat
    17761 agggtttaac gattcggatc tcctcgatct ccagcggagg ctggtcattc tgcgtgtggg
    17821 cctcctgctg gtcgtcgcgt tgctcgcctt gcgtctgtgg catctccaga ttcgggaagg
    17881 cccctattac cgggacctgt ctgaaaacaa ccggacgcgc tcggtggtgt tggagccggc
    17941 ccgcggtctt atcttcgatc ggaacggtgt gttgctggcg aacaatgtgc cgagctttgc
    18001 cctctatgtc acgctggaag acgtgaagga tcggccggcc ttggttgcgc aattggcctc
    18061 gttgctcaac cttgagccgg aggtgattca aaagaagctg tcgaccggca agggcagcaa
    18121 gctgcagcct cgcaaggtga aagaccggct gacgttgcgc gaggcgacat tgatcgagtc
    18181 tcatcggttg gatttgcccg gtgtgatgat tcaagtggag tcgcaacgga actaccccgg
    18241 cgggccggtt gcggcgcatc tactgggata tgtgggagaa gtgtcggcgg atcaacttga
    18301 aaaggcggac tttgccgatc tccatcaagg cagcgtggtc gggcagtatg gagtagagaa
    18361 gtggttcgac cggcaggtgc gcgggcgcgc ggggcagaag agcgtggaag tcgatgcact
    18421 cggccacgaa aaacgcgccg tggtgtcgga caagccggtc gccggagacg atctgtatct
    18481 caccatcgat gcccggcttc agaaaaccgc cgaagatctt cttggggagg agtcgggtgc
    18541 catcgtcgcg ctggatccga ccaacgggga tattctggcg atggccagcc gtccgggatt
    18601 cgatccgaac atgttgtcga aagaactcac gaacaaacag tgggttgaaa tcgtccagaa
    18661 cgagcggcgc ccgctgaata atcgggccac gcaaggtcaa taccctccag gctcgacgtt
    18721 caaggtcgtc atggcggcgg cggcgcttga atccaacacc gtgactccct cgaccatggt
    18781 tcggtgtaac ggcggatatc aattcgggaa tcggctgttt cacgactgga agcaaggcgg
    18841 tcatgggtca gtcgatatga acgaggcttt gatccactcg tgcgacgtct atttctatac
    18901 cgtcgggcag cgaatgggga tcgatacgat cgccgagtat gcgaagcagt tcgggctcgg
    18961 gcaggagacc ggagtcgaac tcccgtccga acgtgttgga atcgtgcctt ccacagcctg
    19021 gaagcagaaa gcccgcaacc agccctggta tcccggtgaa acgatttctg ccgcgattgg
    19081 gcaggggtat gtcactgtga cacccttgca gatggccagc gtcatcggta cggtggccaa
    19141 cgacggcctg tcgatcaaac cgcgtctggt gcaggcggtg atgaatcgtg cgacgggaga
    19201 acgggccgaa ttccctccga cggtccgggg caaggtggcg gtgaagccgg ccaccatcgc
    19261 tttgatcaag gcggcgctgg cggatgtggt gaccaaagga accgcgacgc gagcgaagtc
    19321 gtccctcgtg acaatcggag ggaaaaccgg aacggctcaa acggcggccc tccgtaccgg
    19381 tccggagaaa gatattccca aaaggcttcg ggaccatgcg tggttcgtcg cctttgctcc
    19441 cgtggcggct ccgcgcattg ccgtggcggt cctggctgag cacatggggc atggcggatc
    19501 cgccgcggcc ccgttggcga aggatgtgat tgaagcctac gtgaaggcct atcccgaggt
    19561 cctcaatgga gttcctgctg gtgggcggac gaaatccgct gacgcgcgac caaagacgtg
    19621 atgattgatc gggtaatcga cagtcgaggg ttagatagtt tcgacctccg tttcatgggg
    19681 ttgatcgcgg tcatcctgtc ggtcggggtg ctgtcgatct acagcgtcac gcattctcag
    19741 gacaccgcct ttccttttta tctcaaacag ctcgtgtgga ttctgctggg cacgatcgcg
    19801 ttcctggtga tgtacctgtc cgactatcac aagattgcgc gcctggccta tccgacttat
    19861 gcggtgattc tgattatgtt ggcggtcgtg ttggtcatgg gtaagtccag ccgtggtgcc
    19921 caacggtgga ttccgatcgg gcccttcgcg ttccaaccgt ctgaatttgc caaactggtc
    19981 ctggtgttgg tgctggcgaa ctattactcc cgggtgtctc gcgcggggtg gctgcaccgg
    20041 gtggttttgc cggggttgat tgtgttgccc ggcctgctgt tgatcctcaa gcagcctgat
    20101 ctggggagcg ggttgagttt tctggccgtc tacgcggcca tgttgttgat ggtcggtgtg
    20161 cgctcgaaga cgttgggggt gattttgctg ctctccgtca tgctcttccc gtttgtatgg
    20221 gaaatggtct gggcttccct gcacgactat cagcgggagc gtgtcatggc ctttgtcgat
    20281 ccagagtacg atccaggagg aaaaggctat catgcgctcc agtccagaat tgccatcggc
    20341 tccggggagt tgtcgggaaa agggctgtac ggcggcacac aaagccagct gaagttcctt
    20401 cccgaagggc ataccgactt cgtctttgcg gtgtacgcgg aagaatgggg ctttgtcgga
    20461 gtgctggtgc tcttggcgct gtttattgcg ctgatatggg tctcattgga aattgccgcg
    20521 cgtgcgaagg acaccttggg ggcgttactc gcggcaggga ttgtggcgat gctctgtttt
    20581 tgtgtggtcg tgaacatcgg gatgaccgca gggatgtttc ccattgtcgg cattccgctt
    20641 ccgttggtca gttacggagg gagcgccacg atcatgacca tggcctcatt gggattattg
    20701 ttgaacgtca aacgacgtcg gttgaccttg ttctattagg ccatgcctgg acaggggccg
    20761 actgggtatg aaataaccat gtgaaccaga ttggtgccgg actgacggcg agacgaggaa
    20821 gaacccgggc tgtgcgtccg cgttcgtctg ccgatccgcc cgatctgagg gtaaaggagt
    20881 ttctatgggt gtggagatag caatcagtat agcgcgagaa gaaacccgcg tggcggtact
    20941 cgacaacggg gttgtcaccg acctctatgg cgaccgcgcc aaagaccagg atttcgtcgg
    21001 gaatatttat aagggccgtg tggcgaaagt gctgcccggc atgcaggcgg cgttcatcga
    21061 tatcgggttg gaaaaagcgg cattcatgca cgtctccgac ctctcgatgg atgtcgaacc
    21121 cggggatacc ctcgtagagg gcgacgacga cgataacaag gactcggagg tccccaagcc
    21181 aaaacggcaa agttcaaaac ccattgaaga gttgctgtcc gagggacagg aagtcatggt
    21241 gcagatctcc aaaggtccga tcggcaccaa gggatcgcgc gtgacgacct atgtgtcctt
    21301 gccgggccgg tacctggtgt ttatgccgac ggtcgagcat atcggcgtct cccgtcgtat
    21361 ccctcgtgat gaggagcggg cgcgattgaa agaaatcatg aagcgggcgc gccgcccggg
    21421 gttcggctac atcgtgcgga cagtcagtga aggggtcaag gaagaggagc tgcgctcgga
    21481 cgtcgagttc ctgcatgtct tatggcagga cgtattgacg aagcgtgatc agcagccggc
    21541 tcccagtctg ttgcacagcg atttaagtct gagcttccgc gtcgtgcggg atctcttcgg
    21601 tcgtcgggtg gaccggttat tgatcgattc ccgcgaggag tacaacgcga tcaaggggtt
    21661 cgtgcaacgg ttttcgccgg agcaaacctc gcggattcat ttctacgaca aagaagaaag
    21721 cctgttcgat tacctgggcg tggagcagga aattgcccgt gcgatgagcc gcaaggtgtg
    21781 gctcaagtcc ggagggtatt tggtcatcga ccataccgag gctatgacgg tgatcgacgt
    21841 gaataccggc cggtttgtcg ggaagcgcga tcaggaagaa acgatcctca gaaacaacct
    21901 ggaagcggcg cgcgaaatcg cctatcaggt gaagttacgg ggaatcggcg gcatcatcat
    21961 cgtggacttc atcgacatgg agcgggagaa aaatcgcgac aaggtttacc atgcgctggt
    22021 ggatgccatg tcgtctgata aagcccgcac cagaatttcc agggtgtccg atctcgggtt
    22081 gatcgaaatt tcccgtgagc gtgtgcgaga ggacttgctg cgttcgttgt cggagccctg
    22141 ccactattgc gacggtcgcg gctacaccaa gtcgccgatg tcggtggcct atgagatttt
    22201 ccgtgaagtg cggcggctcg gccatgagat cgagcagcag cgcgtggttg tcggcgcgca
    22261 tcctgccgtt gctctgctct tgcaggaaca ggagcaaccg ggcgtcgagg aactcgaacg
    22321 gcgatatagc gcaaaaattc tggtcacgcc cgacgatcgg ttgcatctgg agcaattcga
    22381 tctggtcgtg atctaatccg ccgagcgacc gggcgtgtac ctgttccggg tgggcatcct
    22441 gccgctgaac gcggtctttc tccagtacaa cgggcatgac atcgcactga cgacgtgtga
    22501 tgagttgaga cgatagtgag tcatcgatct ctccgtccct ggttggtgtt gcgcgcgatt
    22561 cccggtgtgg gcgacgccat ccttctcaag cttgtgcatg cattcggggc gccggatgct
    22621 gtgctctcgg cgccgcagtc ggcgttggag gagattggat gccggccgcc tctagtcgag
    22681 gcgattcgtc gagggccggc tccggatgcg atccgggaac tcgacaagga attagagcag
    22741 ctgcagcgcc ggaaggttac ggtgctgacg tatcttgatg cacagtaccc cgcgccgctc
    22801 aggacgattc cggacccgcc cccgttgttg tacgttcagg gctcgctgct ggaaaccgat
    22861 cggcatgccg tggcgattgt cggcacgaga aaagtcagtg ccgccgggcg catcgtggct
    22921 gaggagttgg cgcgcgactt ggcgaagatg ggttttacga tcgtcagtgg tttggcgcgt
    22981 ggagtcgatg cggccgcgca tcgcggggcc cttgccggta aggggcgaac gcttgccgtc
    23041 atgggctgcg gactggatcg gacctatccg gctgaccatc ggcagttgcg ggagacgatt
    23101 gaacaggagg gagccgtgtt gtctgaactc ccgttggggg cggcaccgca cagctatcac
    23161 ttcccgcggc gcaaccgcat catcagcgga ttgtcgttgg gcgtagtagt gacggaagcg
    23221 gccattgaga gcgggtctct tattacggcg agattggccg gtgagcaggg gcgggaagtg
    23281 tttgcggtgc cgggttttgt caaagctgaa cagagtcgtg gcccgaacag tttgatcaag
    23341 gatggtgccc gcttggttga gtcggcgcag gatattctgg aggaattgtt gccgcagctt
    23401 gatccggcct tccgtgagcg gctggccggg ggagcaggga ccgtcgccca gccgaaggtg
    23461 caattcagcc cggatgaaac gttggtatac gatgtcttgt ccgtcttgcc gcaatcggtc
    23521 gacgatgtga ttcggcgcag tggattgcct gccgctcaag tggcggcgat cctgttatcg
    23581 ctcgaattga acaatagtat tcgtcagcta cccggcaacg agtacgtgcg tgtcacatcg
    23641 ggcatgcatt gagagattag agctgacaag ggctcgatat ttgttatagt gggtgtggta
    23701 gtctgggcgt aggtactgag gaacgtggag ccgcatggcc aaatcgctga tcatcgttga
    23761 gtcgccgacc aaggcgcgga ccatcacgaa atatctcggt cgtggctact ccgtcatggc
    23821 ctctgtcggc catgtcaaag acctccccac cagcaagctg ggcgttgatc tcgaaaacga
    23881 ttttgaaccc cagtacgtca cgatcaaggg caagtcaaaa gtccttgccg acattaaaaa
    23941 gaaagcccag gaagtcgaca ccgtgtttct cgcgccggac cctgatcgcg agggcgaggc
    24001 gattgcctgg catattgcgc aggaactgca cggcaaggga aagaagaaag acggcaaagt
    24061 ctatcgtgtg ctgttcaatg agatcacgga atcggcgatc aagcgggcac tgaagtcgcc
    24121 gggtgaaatc gatatgaagc tggtccaggc tcagcaggcg agacgagtcc tcgatcgcat
    24181 cgtggggtac cagggcagtc agttgctgtg gaaaaaggtg cgccgtggtc tcagcatggg
    24241 ccgggtgcaa tccgtggccg tgcggttgat ttgcgaccgg gagaaggagc gcgaagcctt
    24301 ccgggcggaa gaatactggt ctatcgttgc gttgttggct ggggacaatc caccagcgtt
    24361 cgaagccaaa ttgcattccg tgaacggtca ggatgccgat atcggcacgg gcgagcgggc
    24421 ccagcagatc ctggatgccg tgcaggggaa gccgttcgtc gtgcaatcga tcgagcgcaa
    24481 ggaaaagaaa cgcaatccgg tcgcaccctt catcacgagc cggcttcagc aggaagccgc
    24541 cagaaagctg catttcagcc cgaagaagac gatgacgctg gcgcagcagt tgtatgaagg
    24601 cattgaaatc ggggcagaag ggcccaccgg tctcattacc tacatgagaa cggactcgcc
    24661 gaggatttcg caagaagcga ccgaagacgc cagagttgtg attcgggagc ggttcggagt
    24721 ggagtatctg cctgctacgc ccaacgtgta taaaacccag aaagccgcgc aagaggcgca
    24781 tgaagccatt cggccgacgt ccgcggcgcg cgatcccgaa tccatcaggc aatacctaga
    24841 gcaggaccag tacaacctgt ataagctgat ttggaatcgc ttcattgcct cacagatggt
    24901 ccctgcgatc atggatgtga cgcgtgtgga ttcgtctccg gtgggaactc cggatcgata
    24961 tgtgtttcgc acgaccggca ccgtggtcaa gttcgccggc catacggcgg tgtacttaga
    25021 gggaaccgac aaggaattgc cgtcgcgcaa gcccaagaat gagcaggagg cggaggacga
    25081 atcagatcgc cagctgcccg cattgcgcga aggtgaatcc ttgcatctgg tcgctcagga
    25141 agcccagacc ttgcccgggc tcgtttctaa acagcatttc acccagccgc ctccccggta
    25201 caatgaagca ttgctgattc gagagctgga agaaaagggg atcggccggc cctccaccta
    25261 cgcttcgatc atttcgacga tccaagaccg gaagtatgtc gagaaggtcg aagggcggtt
    25321 tctcccgacc gaaaccggga gaacggtgaa tgactttctg ttgaagggat ttccggatct
    25381 gctggatacg gaatttacgt cccacatgga agagcagctc gatgaagtcg aagagggcac
    25441 aaagccctgg gtttcagcgg tgcgcgattt ttacacaccc tttgtgaggg aaatggaact
    25501 cgctcaaggt atccctggac cgaaggatat agtcgagccg cccacgaacc ttccctgtga
    25561 aaaatgcggc cgcatgttgg agatcaaatg gggacggaat gggaagttcc tcgcgtgccc
    25621 cgcctataag gacgatcctc cctgcaaaaa tacgcaaaat tttgaaaagt tgccggatgg
    25681 gacgattaag attgttccca agctggaaga gacgacggac gagaagtgcg aaaagtgctc
    25741 cagcccgatg gtggtgaaat cgggccgctt cggtaagttc ctggcctgtt ccgcgtatcc
    25801 ggaatgtaag acgaccaaag ccattgctct gggtgtgaaa tgtcctcagc ccgggtgcgg
    25861 gggggatctg gttcagaagc gcacgaaaaa ggggcgaaat ttttattcgt gcagtcgtta
    25921 cccgcaatgt gagtatgcgc tctgggaccg ccccatcaat aagccctgtc ccaaatgtca
    25981 ggcgccgttt cttattgaga aagtcagtaa gcaggccgga cgttcagtgc aatgccgcaa
    26041 tgaagaagag tgcggctatc gggaagcagg atagtcaagg cgcatccgtt cctctgtcgc
    26101 ggttcgctcg tttcgatcag ttgttccgca tctcccccct cgactccagt tcgtgtcccc
    26161 gccgagctct tgcgggacaa gccgttcccg atcttgtcct gatgagaaga atgagaagcg
    26221 ttctcggcga tgttgataac tcgcctcatt tctacgtact tctctagttg acaagcgctt
    26281 cgagaatctc taattaaggt gtagagtaaa gagtcggtcg atcgcacgtc agcgtcggcg
    26341 tggaccaaca tgccgacgga aagcattgcg ctccaaggct ggagggaggg acctatgaaa
    26401 gccaaacaag gggtcattga gattctaaat aaagtcctca ccgcggatct caccgcgatc
    26461 aatcaatatt tcgtccatgc gaaaatgtgc tcgaactggg gttatgagcg gctgcatcac
    26521 aaggttcgag aacgtagcat cgatgaaatg aaagatgcgg acgaactgat cagtcacatt
    26581 ctgtacttgg aaggggtgcc gaatgttcag cgtatgaaca cggtgcatgt cggggaaact
    26641 gttcccgagc agttgaagct tgatctcaag gccgaacagg aaatgctcac ccttctgagc
    26701 gacggggtgg tgcattgtac gaaggtggga gatttcacca cgcgccacat gtttgaagac
    26761 atggcgaagg atgtggatga gcacatcgac tggatcgaga cccaaattga gacgatcaag
    26821 caagtggggc ttgagaatta cctggctgag cagattcaca aagagagtta agtgagcgcc
    26881 ggcccggcgt gggaatggga gtatgcatga aagcgaaaga gggcgttctc gatattttga
    26941 acaaagtgct ggtgagtgag ctcacggcgg tgcaccaata tcttctgcat gcggccttgt
    27001 gcaaacattg gggctacggc cggctgcacg aacatttcaa tcacctggct caggaggaag
    27061 tgagtcattc ctcgggtttg atcgatcata ttctgtacct gggtggcgcg ccggagatgg
    27121 atcatttgga tgcggttgcg gccggcaaga aagtgtcgga tctgctggag gccgatctgg
    27181 ccttcgagcg tgacgacgcg gacgtcctcc gcgacgggat tgcccattgc acaaaggtcg
    27241 ctgacttcac gactcgtcgt cggctcgaag agatgatcgt cgatacggaa gagcatatcg
    27301 attggtttga gactcagctc tgcaccatca agcaagtggg tctcgatcga tacttggccg
    27361 agcagattca ttctgagaag tcttagccct tgcgtgcgca ggtggtcggc cggtgggatc
    27421 cccgaaggcc ccgctggccg gctctctcct ctgtcgtctt ctcgccccac gcacatgctt
    27481 cgctgaaccc cgccgtccgg taatttgaat cacccgcgca ttcattctcc cctcactgct
    27541 gcgaagcgac tcgatcgttc tgtattccat ggccattcag ggcaggggac attcccgttc
    27601 tccaagcagt gaagccgtgt ggttggtctg gccgctggct acagtgcttt ctgcatggcg
    27661 tggaccgtca gccgatgccg tgagtgtgcc tgccgggatc gaatctccct gggagaatgt
    27721 ccggtcgcgc gtttgaagaa atggctgaag gcaaactgat cgctgaaccc caccgctgta
    27781 gcaacttctg ccaacgtctg cgcggttgtg gtgaggagtg attgagcccg ttcgacccga
    27841 tgtcgtctca tgtacccgga aaacgattca cccatgatgc ggtaaaacag atcagagcaa
    27901 tatttctccg aatagccgag gaaatttgcc aggactttga gtgtcagtcc acgatggaga
    27961 ttttcgttga tgaagcgggt gatgcgtccg gccagcgtgg ttgtctcatt agcggactct
    28021 ctgtgggacg gtagggcatg ggacaggtta aagagatcgc acacgagatt gcgtagctcc
    28081 tgttccgtcg tggcctgctc aagacgatca gcccagtgtg gtgactgtgc gcgaagctcg
    28141 gacgagaggg tgcggaggcg atcagttgga atttcgatgt caggcgcgtt gttcatcatc
    28201 attcagctcc atcagtgagg ccaatagccc gtacgccggg tatggactgt tttcgttcga
    28261 ctcctcagaa cattgaaaag ggcatatgct ggtgactcgg ttggtcgagg gtcggttcaa
    28321 cagtgagagc ggtgacactc gcgtcaccga atggtagcgg cgggagctgg tcttgatcgc
    28381 gaacccgtgc acaaaatgtg gcggtcagta tcggatcaaa ttgtgagcct gccgacgctt
    28441 gtagggttcg tagggccctg tgtaacgaag tcgatcgatt ggcaatgctg tcgaagacgt
    28501 ccgcaatggc caaaattctg gcggccaagg ggatatagcc tccgcgaagt ccataaggat
    28561 atccggcgcc gtcccatcgt tcgtgatgat gagcgatcag gcgtgcggcc tcagagagga
    28621 atcgatgggg gctcaggagc gcagcgcctt cccggggatg gctttggatc agggcatagt
    28681 catccaagct gtgcgctgct gtctcgtcga gcagtctgcc gggtaacgtg aggagtccga
    28741 tatcgtgcag cagggctgca tagtgcagct cgatgtgctg gtcatccgta aggctgatgg
    28801 ccttgcccag caagcgggca tagtgtgcgg tgcgctcgcc atgtccgaag tgtccaggaa
    28861 tgagggtttc gatcctccgc gtcaggcgtc gaagttgtaa caggcgaaca gcgtgtaatg
    28921 attctttgga aagctgaagt gaatccaagg catcaagctg tcgggttatt tcctggatga
    28981 gctgtgttgt ggcattgatc ggcgatatcg atggatgcat gggaatcctc cgaaaaaaaa
    29041 gagcccaaga tcgttgcaca cgatcttggg ctctgggctg aatacctctg gccgcaacgg
    29101 agtcaacgga cttcttctag gcgttcatga ctcttcccct cgcttcccgg ccatcagcgg
    29161 ttgcttcaaa atccacattt cgtagagagg gatagccatc atcgcgagaa agctgcccat
    29221 catcagagtg tccccggcca gcattgtgag cacaaaaata ggtgagatgt agctgatcag
    29281 ccagggggag agcaggtcca gcgccacgcc tccgaacgaa agccatgtca gccagacttg
    29341 cgtggcatga ctggcctgcg tgagatagag gacatggacg agaatcataa acacgaccgg
    29401 catggtgaac aggtgaaagt gcgtgacctc ggccagctcc cgaaacgaca tcggttcgcc
    29461 gaaggtcgca tcagagccac ggtagtggtc ggcgatacct tgtgggctca agctggtcat
    29521 gctgtgcgcc cagaagaaag aaaaaagaaa ccccaccagc atcaaaaaga ggaattgcgt
    29581 atacaacaac ctgatatgac gatccgaatc acgcaggcgg aatcgagcat taaaatttcg
    29641 catgccagtg cagtgtcccg acggccttag ttgccgaata gggtccccaa cagtcctctc
    29701 tcggccttct gcactgcgac cgtgtcgctg cctaagccgg ccggcttcaa gtaaaattcg
    29761 tctaccagta ctaacactcg tttgacgccg gcgttcatcg accggacgga catcgtggct
    29821 ccactgatgt tgataatgtc tttgttgatt cgaatggggt ccgtgacggt cttgccatca
    29881 tactgagagt tgaatcgctt cttcccgacc tcgctgccct ttgcatcccg aaacaccagc
    29941 aattcgacgt cggtgcaatt ccctttggga tccactccta ccatataggt catgggttta
    30001 tatttgccga tggtgttgtg gatcatcgca tacccgtcga ctttgtctcc agtctccccg
    30061 atatacagat cgaacgagtc ttccggaaac ttccaaccga tgcgctgttc gatgagttcc
    30121 ttcctgtcct gcgtgagtcg gatcagttcc ttgcgcacac gttgagattt tgggaggatg
    30181 gtcttcacgg cctcctcttc ggtcatgaac tcttcgtact tgaagtcgtc gtctttcagg
    30241 aagcggcgca attcgttgtc ccagactttc tccgtcgcca gcgccgggag cgacgaggtc
    30301 agaagcaata cgactgcggt caaggtgcgg tacgcactca atgtactgaa catccatgct
    30361 ccttcaattt ggtgtctaag cggttctgca cgtcggtcat gacttgggcc gaggactcgg
    30421 tgtcggtcca gccgaacagg cgagagccgc cacggaatgc ccaccgttgt cgctcttcga
    30481 tgaagccgat ttttgtgaca tcgtcgagtc gtttgactgc cagggaaatc cgggaccgat
    30541 ctttgctatc ccccaagtct cgctgaaggg caccaaaggt ccgtcccggc atgggtattt
    30601 caagccacgc ggtgtcgatc aggcctgcgg ccttatcctg ccgcgctatc gcacgatctt
    30661 tgactgcgtc aagtgcagct tcccaggcat ggtcgtagct gcagacggcg aatcgttcct
    30721 gcataccgcc cagcgatgcg caggcggtca ggagagcggg aatcaggagg acgctgagtc
    30781 ggcggtagtg ctgtgtcata tcaattctgt cggtatcgtc caggccgtcc cgatcgggac
    30841 ggcctggacg aggaaccggt tagaagtacg tggctgcttg gattaagaat ccgtttccat
    30901 tcatcgggct gcctgcagtg ccgagatccc cggcggctac aaggcccttt gcgtccctct
    30961 gggtattgaa ttgccagtcg aatttgatca ccgtgtcttc aatcggccgg aaattgagac
    31021 cgagggtcaa gcgctcaagc ttccgttggc ctcccagcgc gttctgcttg gtgcgatcgt
    31081 cggtatccgt gttgacctgt tcccatcgca cgactgccgt aaacgtagag gcgtcggtga
    31141 aatggctggg cgcccatttc ttgaggaatt ccggcatgaa gtgataattc ccctgaacgt
    31201 agtagccttg catgcc
//
LOCUS       DAOQRM010000013        30088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_909_length_30088_cov_14.030933, whole genome shotgun sequence.
ACCESSION   DAOQRM010000013 DAOQRM010000000
VERSION     DAOQRM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 30088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30088
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_909_length_30088_cov_14.030933"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..657)
                     /locus_tag="PKK81_02480"
     CDS             complement(139..657)
                     /locus_tag="PKK81_02480"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNV31764.1"
                     /translation="MNLVLIGYRGTGKSTVGKVLARRLGRTVVSTDAEIVKRAQLSVP
                     EIVKQFGWDHFRDLESAVCRDFAARDQLIIDTGGGAILRPENVDALRRTGTLVWLTAT
                     VETITRRIGGDTQRPSLTGTKSFTEEIRDVLNERTPKYQAAANHVVSTDGASTTEVAE
                     RILQLISRGSAV"
     gene            complement(724..1578)
                     /locus_tag="PKK81_02485"
     CDS             complement(724..1578)
                     /locus_tag="PKK81_02485"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNV31765.1"
                     /translation="MDITTQTQWCGIIGNPVEHSLSPAIHNAAFQKMGLDLVYLAFRV
                     EALGDALKGVRALGNARGFSVTIPHKVAAIPFLDEVEPTAKHIGAINTIVVEEGKLKG
                     YNTDASGALRALKEGGALLDGHRVLILGSGGAARAIAFALATGTGIAGLTILGIEEVE
                     RQKLVKDLREKTNLPIEDGPLTLDMLRNWVPHVRTLIHCTPVGMSPRVEESCVPTNLF
                     RPELTVMDIVYNPRETKLLQEAKAAGCRTISGLEMFLNQAVLQFELWTKQPAPADVMR
                     RVLESRFG"
     gene            complement(1592..1927)
                     /locus_tag="PKK81_02490"
     CDS             complement(1592..1927)
                     /locus_tag="PKK81_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31766.1"
                     /translation="MSGMSRMSAVVVPALFLAAVLSGLLESSVEPVRAEGGTEFVGTI
                     LSVDTTSGKFAVKKDGGGSRFTFVANEKTHFDGGAKAVKDLKKDDHVAVLYQVQGSQY
                     VALNVALKK"
     gene            complement(2018..2779)
                     /locus_tag="PKK81_02495"
     CDS             complement(2018..2779)
                     /locus_tag="PKK81_02495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV31767.1"
                     /translation="MNRLQGKVAIITGGNAGIGEAIAKLFADEGASVVVTGRRKEELD
                     RVVKGIGVNGGRALAVVGSVTDEAHVQDVVAQTLRTFGKLHILVNNAGIGDFGKRLHE
                     IDDATWAKVLDINLTGVFRMTRAVLPELLKGGGGSIINISTVASLVGIRGLSAYAASK
                     GGLDSLTRSIAVDYAQDNIRCNVMNPGLVDTPMAAPLMADPASLEPIMAQYAIRRPGK
                     PEEVAKMALYLASDEATWVTGATFPIDGGMTISKG"
     gene            complement(2776..3654)
                     /locus_tag="PKK81_02500"
     CDS             complement(2776..3654)
                     /locus_tag="PKK81_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNV31768.1"
                     /translation="MSQLQNGERVHLVDKKGRQYALTLKAGDIYHFSGETIPHDELIG
                     KPDGTVVTLSKGKRFLALRPTFGEYVLKMPRGAQVLYPKDLSLIPMWADVYPGARVFE
                     AGTGSGALTMALLRAVGPTGLVVTYEARDDFARTALTNIERYMGPVSTLMPCRKNAYE
                     GIDVLEDGRLFDRLVLDLPEPWQVVPHAAKVLRSGGIYLSFVPTIPQVMQTVDALERT
                     SVFGMIETFETLLRTWSIQGRSVRPDHRMVAHSGFLTVARKVEEGWWSRPSKTVEAEA
                     AGEQDDEAHEGEDAEA"
     gene            3953..5212
                     /locus_tag="PKK81_02505"
     CDS             3953..5212
                     /locus_tag="PKK81_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV31769.1"
                     /translation="MKDALAIQDDTDPTVAAEVDPDADDPEASTLLDSIVQGNQAETE
                     ADSEDSAPEKPARSGSSPFLLESLYFRSFGERALLERDEEIALAKQLDLGTRQIRQTL
                     QQAVKAGGRLKRTDRTTECLETLKTVRRLSGLSATVLNQAERALEELQLDATTGGRVP
                     VSVRRELEAALTQLKASRITLETAKDELVRCNLRLVVNVAKHYTGRGLTLLDLVQEGN
                     IGLMKAAERYQHRKGFKFSTYATWWIRQGITRSLADQSRTIRIPVHQTEASNRILRAS
                     RRLVQQLGRQPRLEEVASTMRMRPDRLHETIQAFQEPVALEHRVGDGGTELGELLPDQ
                     QAVPPDAHVNRTELTREMDRILGTLTPREQTVIRLRFGIGEDQARTLEQVGQHLSVTR
                     ERIRQIEAKALKKLKTPMVKEMFAAIK"
     gene            5341..6414
                     /locus_tag="PKK81_02510"
     CDS             5341..6414
                     /locus_tag="PKK81_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNV31770.1"
                     /translation="MLTALYSRNGARPDRTRWFAWGLFSLSLLSLCTPANGAGHNPNE
                     STSLTELSVPAVVAKVRPSVVTVLTRGIPQGGSQHGAPPGSGSGVILDAGGHILTNNH
                     LVQGVTSVVVGLSTGRLTPGRVVARDFLLDLALVRITAPDLVPAEFSRRASFEIGETV
                     IAIGNPLALKGGSTVTVGVISALDRSVLTPEGETLYDLLQTDAAINPGNSGGPLVDRY
                     GQVVGINVAIAPSAQAISYAIAMEAVTPHLQSMMDRGSVLRPDLGLIPLTITPSVAAS
                     FDLESDRGILALSVDPAKPAGRAGLQSGDVVTAIDNHPLYNIGDFWHAVSRASDQLSF
                     QITTQGKAGTGTITVSRPGPARP"
     gene            6418..7245
                     /gene="lipB"
                     /locus_tag="PKK81_02515"
     CDS             6418..7245
                     /gene="lipB"
                     /locus_tag="PKK81_02515"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNV31771.1"
                     /translation="MKLDQPSQTESAHPVSKVPAQAQPPRAGILIRCEPLPYEIAWSL
                     QRACRTERAADRCEDLLLLLEHPPVFTVGRTTQDQHWPGESCLTQESGIPVIRTERGG
                     SITYHGPGQLIGYPVLRLSDFCAGPKAYVHRLEDVIISTLAEWGIAGHRRERLPGVWV
                     RGDVPSKIASIGVRISQGVTTHGFALNVCLDLAPFSHIVPCGIADCRVTSMAALLGEA
                     PEMGAVQERLAANFAERFHLAWAERIGPDRFLALSDASPTIAQGPLPLRATHQQGVT"
     gene            7245..8501
                     /locus_tag="PKK81_02520"
     CDS             7245..8501
                     /locus_tag="PKK81_02520"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNV31772.1"
                     /translation="MNEFDTHTFRLAVAQFNEAAESMRLDTNLRERLKLPQRSLLVSI
                     PVKMDDGHVEVFTGYRVQHDSARGPCKGGIRYHPDVNLGEVAALAMWMTWKCAVADLP
                     YGGAKGGVKVDPKKLSRGELQRLTRRYAAEIFPLIGPDKDVPAPDVGTDQQVMAWIMD
                     TYSQQVGYAVQGVVTGKPLSIGGSLGREEATGRGVSYVTLEALQHLKLDVSKATVAVQ
                     GFGNVGSNTALIMQQAGARVVAVSDVGGGLYNPKGLDIADVLHRYRDKHEPLREIKLG
                     ESISNEELLQLDCTVLVPAALSEQITHANASKLRCRILAEGANGPTTLEADRILTDKG
                     VFIIPDILANSGGVIVSYFEWVQDVQRFFWKAKDIQDRLQDIITSAFHRTLQFSVEKR
                     TTMRMAALMSGIDKVAQAHLQRGLYP"
     gene            8533..8919
                     /locus_tag="PKK81_02525"
     CDS             8533..8919
                     /locus_tag="PKK81_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31773.1"
                     /translation="MTRYILAAIAGIWMADGLALLTVPLLVVKRVQESLLHSPQLLRW
                     EAVGIGLGAILILWSGPIPYQPLWWITGGAMIIKGCFLTWGPAAWRTPLLDWCFAREA
                     IDYRFFGLWLCMLAVLLLHALGLLHR"
     gene            8956..9249
                     /locus_tag="PKK81_02530"
     CDS             8956..9249
                     /locus_tag="PKK81_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31774.1"
                     /translation="MSHQTIASLWEEHSREGWPRFSSPNEGQLMTLDTVIGGCAVFYL
                     DGQTEIDSQRIAILQDCVADLDTLLDDLTEDSLGYFQRLRRLAAALVQLNQAT"
     gene            complement(9310..9768)
                     /locus_tag="PKK81_02535"
     CDS             complement(9310..9768)
                     /locus_tag="PKK81_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HNV31775.1"
                     /translation="MGAIVLSALAVLAGCADTGSLNGAGHPAAGATATKSLYDRLGGK
                     PAITAVVDQFVANVAADGRINSRFATTDIPKLKGHLVDQVCGATGGPCSYHGRDMKST
                     HLGMQISSADFGALVQDLVAALDKFNVPAGEKSELLGILGPMKKDIVEVP"
     gene            9971..10918
                     /locus_tag="PKK81_02540"
     CDS             9971..10918
                     /locus_tag="PKK81_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNV31776.1"
                     /translation="MELRQIEYFMAVAAHQNFSRAAEHVHVSQPSLSIQIGGLEKELG
                     TRLFDRLGRKVVLTQAGELFHVHAERALRELDQAEQVVQELLGAKRGRLVVGALSTVN
                     SYLIAPLVSRFKQRFPDIHLQVHAQASADVVSGLLSNRLDIGICLLPLTHPQLTTVPL
                     FEERLALVAPAGMNIGKRRARIQDLARLPLVLMPVDYCLRKMVEAECAKAGVHPQVVL
                     EMSSPEGILQAVAEGTGATILPELYVRSRLPGMDLQVIDLYDPTPRHAVGLAYLTKRH
                     RGLAAEEFAAICHTTMRDLQSDPEQSRRNRPTRRAAGQK"
     gene            11095..11625
                     /locus_tag="PKK81_02545"
     CDS             11095..11625
                     /locus_tag="PKK81_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31777.1"
                     /translation="MMQRKWAFTALVAMMGSLYLAAMVSAGPYELSKDDLTDPKGIAS
                     PDVSLFGIKLGDDEGKALEGLVNEKIPGIKAEQEATFIFLLDQRKPTGPMAGVRVQDG
                     KVDLIFINNRFAYKTRGIFRNVLNSESPDDVRKLLGKEDYGDENVMGAILAYDKQGFQ
                     VNYLGKDVNVEFSLPH"
     gene            complement(11780..12709)
                     /locus_tag="PKK81_02550"
     CDS             complement(11780..12709)
                     /locus_tag="PKK81_02550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNV31778.1"
                     /translation="MNRLRRTLSIGAFGILALLFGLWSGQALALDSDVDDEVITVPFD
                     PRPVPSAKQANHSLRWRHVPAHSILLKELKTGTTLYQFESEKRLSPASLTKIMSALVI
                     LEYGHLDDEVTVSPKAARAHKTHLRLRTGQIFRLEDLLKAMLIVSANDACLAASEHVG
                     GDEARFVDLMNAKAAALQLHNTHFSNACGFDAPEHYSTAEDLAKLSEIALHHPVFKEL
                     VREEREIIMPINEHRAYVLRTTNRLLGRIPGVQGVKTGFTSKAGRCLIAKVSQDGSDL
                     LLVILNSKRRWNTATSLINYGLRLSDSRAGLAR"
     gene            complement(12755..12943)
                     /locus_tag="PKK81_02555"
     CDS             complement(12755..12943)
                     /locus_tag="PKK81_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31779.1"
                     /translation="MHCLKCKGFMMVERHYTLMNRRLYARCLNCGFWIDLADLLRFFH
                     KVISSSLRGTKVRETYTL"
     gene            complement(13023..13196)
                     /locus_tag="PKK81_02560"
     CDS             complement(13023..13196)
                     /locus_tag="PKK81_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31780.1"
                     /translation="MGCLTTLAVLMVQGGVRDLVSGTNPAGSSGAILSFSTTVFGGGL
                     LAGCLALILNRLR"
     gene            complement(13365..14561)
                     /locus_tag="PKK81_02565"
     CDS             complement(13365..14561)
                     /locus_tag="PKK81_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNV31781.1"
                     /translation="MTADRRGIKIRSFFGVGPVLLAGLIMLSACSKADPYVPPDPFYY
                     FASYPVGKNPTTVTTADFNRDQITDLITTNISSNTISLLFGNGDGTFRDQVQVKVCQE
                     PRSLAINDFNHDDQLDVVLACSGSDQVSILFGRGNGKFEDGPQYPVHRTPVSVSSEDV
                     NGDGHPDLAVALRNDKVKIFLGSAGGTFRPGFQYEYGDTPTSVALKDLNQDGKPDLVV
                     TNGGPMLSAVSVWIGNGDGTFRDPKDYKTGRRPLGVSFADFNNDSLPDLLVINGEGDS
                     FTTLLGNGDATFQPGKDSGADASPNFGAAHDFDGDHIADVAIVNLQSTDLSILFGRGD
                     GTFHYPPRNYRTRNGPFAVASYRVSADNMEEPGLVTADNGAGSVSVFLHHGRKGQASA
                     AGRADE"
     gene            14808..15128
                     /locus_tag="PKK81_02570"
     CDS             14808..15128
                     /locus_tag="PKK81_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31782.1"
                     /translation="MSKTANIPLSMYGVAEVLKWCIDRNNGRVSGVDTDGFKKMQALL
                     AEKPTSNDYLALDQFWKKQVMLELTEDEVSTIDRCLYDIPNFDNEPLPQIRHKFWPDA
                     VGAH"
     gene            complement(15411..16406)
                     /locus_tag="PKK81_02575"
     CDS             complement(15411..16406)
                     /locus_tag="PKK81_02575"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HNV31783.1"
                     /translation="MAESSNITAPEVRWSDGALKRMERAPMFLRGMVRRLAEKKAREL
                     GYPEITEEILDQFKGQMMGTMGGEGGMAEAADQMAKGQLPWTAAAKERLAAVPEFMRG
                     MIKQIADEIARKGGHMEVNVDLFEKVEALGEIREREAAPLEWSEGALALLQQKIKESP
                     PIAMDFVSDMIRHDTEELAREKGFTRIDEQTAVQLWEAPQERVAWSDEAWKRLQTSPD
                     FVRSGIRKAAERRARKLGLKEVDSEHLTTFRNQAMMKAVKRIRSFGYNELTFDAFDTA
                     LQKTKRLQGNDQAEKRLQEIRSHFSDPEVKKPEGGTLGADLMGRFRKFLKGEGSL"
     gene            complement(16420..16698)
                     /locus_tag="PKK81_02580"
     CDS             complement(16420..16698)
                     /locus_tag="PKK81_02580"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNV31784.1"
                     /translation="MTKAQIIERVSEQVTTLTKRQAEIVVNTIFDCIRDSLRNGNKTE
                     IRGFGSFRLRARRMKEGRNPKTGATVAVPAKRVPFFKAGKELKELLNK"
     gene            complement(16772..17671)
                     /gene="sppA"
                     /locus_tag="PKK81_02585"
     CDS             complement(16772..17671)
                     /gene="sppA"
                     /locus_tag="PKK81_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNV31785.1"
                     /translation="MGPQADTAVRPQRSLLRRIFWAIVIGGGALILLNALLPDLDFSS
                     QDRVALIRIEGVILDAQATISELKQYSENPLVKAIVLRIDSPGGGVVPSQEIHDAVKR
                     VKNKSNKAVIASMGTVAASGGYYIAAATDRIIANPGTLTGSIGVIMEMANFEGLMKKV
                     GVEGVVIKSGRFKDVGSPLRKMSDEERKLLQSVMDDVHHQFIQAVADGRSLEVSDVEP
                     LADGRIYTGRQAKEARLVDELGDLDDAIHIAADIAGMEGEPKVVEPRKRFSFRDIIES
                     RWASVFPKLELNTGVKLKYLMAF"
     gene            complement(17687..19402)
                     /locus_tag="PKK81_02590"
     CDS             complement(17687..19402)
                     /locus_tag="PKK81_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNV31786.1"
                     /translation="MSTVTPQSEPKLDRDALAAMYEETFRNFEEGTITEGMVVAIGKD
                     KVVVDIGYKSEGMIPADQFSHEELAQLKVGDRLQVYLEECEDADGNLVLSKEKADKMK
                     IWEELETLHKEEKSIEGKIISRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGKTF
                     PLKIIKINHRRGNVVVSRRVLLEETRDRRRQTTLSTLKEGQLIQGTVKNITDYGAFID
                     LGGIDGLLHITDMSWGRVGHPSELFQVSDKVEVTVLKYDRETGRISLGLKQKSADPWT
                     GVAAKYPVGTRVRGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEVRHPSRVVSVGD
                     QVEAAVLNIDPGSRKISLGMKQTAPNPWDMIEAKYPAGTRIEGKVKSLTDFGAFVGLE
                     EGIDGLIHISDMSWTKHIKHPSELFKKGQKVDAVVIRIDKEKERLSLGYKQLSRDPWE
                     EQIPNKYRVGDSITGKVSKIADFGLFIELDGDVEGLIHISEVGLDTNVRMEEKFKPGD
                     DVTAKIIKVDRDERKIALSLRDHQMDSDRRQVDEFHASQGGIDQTLGRAAKQSRKRNQ
                     SDSEA"
     gene            complement(19460..20155)
                     /locus_tag="PKK81_02595"
     CDS             complement(19460..20155)
                     /locus_tag="PKK81_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV31787.1"
                     /translation="MSSALYGLLWVISRTIGWLCFRYRTVGTVPRQGGFLIASNHASY
                     LDIPLLGCGIPRRVWYMGRHDLFPMPLLNGLLQALGWIPLRVGRLDRDAFSKAVSLIQ
                     EGKAVAIFPEGGRTMTGALKPGKPGIGVIVSQTGCQVVPAHIGGTFEVLPPGAKWPRF
                     RRVTVAYGEPLDFSADAARLEGKAFYQHVSRTVMAKIAELGQVPIPGDRPANAGQPHD
                     SAATPTAKSCNAE"
     gene            complement(20152..20850)
                     /gene="cmk"
                     /locus_tag="PKK81_02600"
     CDS             complement(20152..20850)
                     /gene="cmk"
                     /locus_tag="PKK81_02600"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNV31788.1"
                     /translation="MGEPGTSGCGKRGLIIAIDGPAGVGKSTVARLLALRLGYLYLDT
                     GALYRAIAWKVRDAGLSPEDQFAITALLPKTTLHMACGPEQSHVLLDGRDITGELRTP
                     AVTALASMVSAIPAVREWLLPVQRQIGAEGCVVAEGRDIGTRVFPEADVKFFLEADAE
                     VRATRRHRELVAAGHSVQFDQTKRDMTGRDDRDRSRTVAPLIPAPDAERIDTSSMPAE
                     AVVEHMLAVITARL"
     gene            complement(20863..22191)
                     /gene="aroA"
                     /locus_tag="PKK81_02605"
     CDS             complement(20863..22191)
                     /gene="aroA"
                     /locus_tag="PKK81_02605"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNV31789.1"
                     /translation="MASLTITPGRPLKGTIAVPGDKSVTHRAIILTALAEGLSQVTDY
                     CRGEDCLNTMRAFQSLGVRIEETPERLTVHGKGMWGLTEPFGPIDCGNSGTGIRLMAG
                     LLAGQDFFTVLTGDESIRRRPMGRVVKPLRAMGATIAGRKGGELAPLAITGTRLKGMS
                     YESPVASAQIKSSLLFASLYADGLTTISEPRLSRDHTERMFAYFGIPFHRDGCTVRIE
                     GRPSIRWSGKTVVVPGDLSAAAFFIVGASIVPDSDVTVLSVGMNPTRTGLLDILRQMG
                     AHIEVLNPREEAGEPVADLRVRSMPLRGVQIGPEQIPQTIDEFPILCVAAAVADGETV
                     ITGAEELRVKESDRIATMAAELRAMGARIEERPDGMVIQGLGRKGANGALTGATCASH
                     GDHRVAMSVAIGALTAAQPTQIQGTVCIETSFPNFDRKLLELLTDSGKRL"
     gene            complement(22198..23094)
                     /locus_tag="PKK81_02610"
     CDS             complement(22198..23094)
                     /locus_tag="PKK81_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNV31790.1"
                     /translation="MMTPHFKQVAIVGVGLIGGSLGMILRRQKLADSVVGIGRRVENL
                     KTAVEVGAIDRYVSDPREGVEGADFVLLATPVDTYERHLREWAGCLKPGAIVSDVGSV
                     KGELVTRAEALLPPSVRFVGAHPIAGKEKTGVAAGSETLFSGARCILTPTAKTDPEAL
                     QTVRVLWELAGSIVLDMDPFLHDKILGAVSHLPHVAAFALMTALADVRDHGLPELDLA
                     SHSGGGLRDTTRIAASSPEMWRDIFLWNRDNVVSLIETYERHLGELKRLIAAGDAAGI
                     EKQLDQAKYEREQLTPRTSGKS"
     gene            complement(23154..24167)
                     /gene="aroF"
                     /locus_tag="PKK81_02615"
     CDS             complement(23154..24167)
                     /gene="aroF"
                     /locus_tag="PKK81_02615"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNV31791.1"
                     /translation="MIIVLKPDASEREVDHIIDRLRELGLKSHISAGQERTIIGVIGD
                     DRILHNQPLTALPGVESVLPILAPWKLVSREFKKENTIIDVNGVKIGDKKITIMAGPC
                     AVERLELTVGIAHEVKSAGATVLRGGAYKPRTSPYSFQGLGREGLDYLVEAKKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYIMSRGNRNVMLCERGIRTFETQYRNTLDMAAIPTLKSLSHLPVIVDPSHATGK
                     WDLVAPMSKAAIAAGADGLLIEVHSNPECALCDGEESIRPSKFKELMGDLRRIAAAVD
                     RTI"
     gene            complement(24186..25271)
                     /gene="hisC"
                     /locus_tag="PKK81_02620"
     CDS             complement(24186..25271)
                     /gene="hisC"
                     /locus_tag="PKK81_02620"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNV31792.1"
                     /translation="MPLKVHPDIASLVPYVPGKPIEELQRELGLPRAIKLASNENPIG
                     PSPKALAVLGETAPTLHRYPDGGAFRLRGALAERWKVTPDHVILGNGSDEILGLLART
                     FLSPGDEAVMAEHTFVIYKMEVKAAHGVAVEVPQKNWHHDLPAMAAAITDKTRLLFVC
                     NPNNPTGTMATKAEVAALMARVPDHVVVVFDEAYYEYVRHPEFPESLSYVNAGRNVIV
                     LRTFSKIYGLAGLRIGYGMTTPEITNYLNRIRPPFNANSMAQRAALAALDDEAHVGAS
                     RALNHAEMDKVRTGLLTLGFEALPSETNFLYFDVGRDGREVFDALLRKGIIVRHIDGR
                     MLRVTIGLPEENQLFLSALADVTRAAR"
     gene            complement(25288..26364)
                     /gene="pheA"
                     /locus_tag="PKK81_02625"
     CDS             complement(25288..26364)
                     /gene="pheA"
                     /locus_tag="PKK81_02625"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNV31793.1"
                     /translation="MSDDLQGHRQEIDRIDDQILRLLNERSKSVIEIGKLKKQRDAEA
                     HLHTPAREAAIFERLSKQNTGPFPTDAIRAVYREIMSASLSLEGPQKVAYLGPRATFT
                     HMACMQKFGSSAQYIPVNSIKDVFSEVERGRAHFGVVPIENTTEGVVNHTLDMFVDSS
                     LLIYGEVLQEVAHHLMSKSGVAGEIKRIYSHPHAIAQCRNWLETNLPHVPVSEVASTA
                     RAAELSVDDPSAAAIASELASQLYGLKVITARIEDNINNFTRFLVLSQKAPERTGRDK
                     TSLMLSVKDKVGALYDLLRPFASHGLNMTKIESRPSRRKAWEYIFFVDIEGHIDEERV
                     KKAAEEVKSRCLFMKILGSYPAYS"
     gene            complement(26504..27541)
                     /gene="ruvB"
                     /locus_tag="PKK81_02630"
     CDS             complement(26504..27541)
                     /gene="ruvB"
                     /locus_tag="PKK81_02630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNV31794.1"
                     /translation="MSDRTVSNQLTDDERGLEAALRPHSLQEYVGQSRMKESLEICIE
                     AAKRRGEALDHAIFYGPPGLGKTTIAHIIAREMGSAIRSTSGLVLSHAGDLAAILTNL
                     QERDVLFIDEIHRLPASVEEALYPAMEDYQLDLVVGQGASTRTVKLELPRFTLVGATT
                     RAGALTSPLRDRFGLVHRLEFYSSQELTSIVTRSAGLLNIPIDEAGAAEIARRARGTP
                     RIVNRLIKRIRDYAEIKAGGRITKQVAQDALVWLAVDAAGLDEMDRRILLTVMEKFNG
                     GPVGVDSLAAAVQEDRGTLEDVYEPYLIQAGFLERTGRGRQATRLAFDHFKKQKDLLS
                     LSDDDTSVTTP"
     gene            complement(27572..28078)
                     /locus_tag="PKK81_02635"
     CDS             complement(27572..28078)
                     /locus_tag="PKK81_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31795.1"
                     /translation="MMKSLSRWWGSRRLVGTVRLVIMAAMVSSGSAGLVGAADGVEEP
                     KSIRKTCGMCPEGYATTGVTHAPELCKDGEPTLVQCVPLGGNMLSVCGTCPEEYVEVG
                     RSNVPSRCGSVDGGLVSQCQLQNMGSTMPDPGQGGVRCPPDCGSTAAPGQGAVSPPPK
                     FRPSPEAR"
     gene            complement(28082..28690)
                     /gene="ruvA"
                     /locus_tag="PKK81_02640"
     CDS             complement(28082..28690)
                     /gene="ruvA"
                     /locus_tag="PKK81_02640"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNV31796.1"
                     /translation="MIALLTGLMAFKAPSQVTLDVHGVGYEVLIPLSTYYSLPNQHEL
                     VTLSIHTHVREDAIQLYGFLTAAEKEAFLLLTGISGIGPKLGLSVLSTLSVRDLFSAI
                     QVGDIEKLGTVSGIGKKSAARIALELKDKVARLHPAGEPAEPGSGRSANRLYEDALSA
                     LVNLGYRQPDVKEALKRIEKSGAMAQGLEGVIREALKDLAKG"
     gene            complement(28772..29527)
                     /locus_tag="PKK81_02645"
     CDS             complement(28772..29527)
                     /locus_tag="PKK81_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNV31797.1"
                     /translation="MGGHSHWATIKRHKSAVDAKRGKVFTRIIRELTIAARSGGDPDG
                     NPRLRLAIAKAKEANMPGDNMKKAIQRGTGELPGVTYEEFTLEGYGPGGTAVLMEITS
                     DNRNRTVAEIRNLLTKNGGNMAEAGAVAWQFHKKGVLVVEKGKVEEDALLSLALEAGA
                     EDVKVTDKAFEVLTDTHDFEAVKKALSDAKIECTLAELNFIPQNTIALEEKAAEQMLK
                     LMEILDEHDDVQKVYANFDISDEVMEKVAAATA"
     gene            complement(29667..>30088)
                     /locus_tag="PKK81_02650"
     CDS             complement(29667..>30088)
                     /locus_tag="PKK81_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31798.1"
                     /translation="WSDHDSWPIGSMTFAQTFFGSDEEVLQHKTYGVLAFAVGVIEFL
                     RRTGWFAHAVWTVPLPLFAIVGGMMLFSHSHGEHPAASRIGLHHVVMGMMAITAGSSK
                     LVSGWRARSVLTERSYWELLWASLVVFIGLQLLIYSE"
BASE COUNT         6103 a         9598 c         8295 g         6092 t
ORIGIN      
        1 ccaaccctcg gatccgaagt ccgatgctct atccaattga gctacgggcg cgcgccggta
       61 taagaaccat gttaccgagg ggatgtcaag aaacgtggat cgcaacggtg gcgttcacag
      121 gcagagggcc tcaacgcctc agacagccga cccacgagat atcagctgca gaatgcgttc
      181 ggcaacctcg gtggtcgagg ccccatcggt cgacacgacg tggttggcgg ccgcctgata
      241 cttgggtgtg cgctcgttca acacgtcccg tatttcctcc gtgaaggact tcgtgcccgt
      301 caatgacggg cgctgggtgt ctccgccgat ccgacgcgtg atcgtctcca cggtcgcggt
      361 gagccagacc agggtcccgg ttcgtcgcaa cgcgtccacg ttttccggcc gcagaatggc
      421 accgccgccg gtatcgatga tcagctggtc ccgcgcggca aaatcccggc agacggccga
      481 ctccaagtcg cgaaagtgat cccacccgaa ctgtttgacg atctcaggca cggacagctg
      541 tgcccgcttc acgatttcgg catcggtcga caccacggtg cgacccagcc tgcgcgccag
      601 cacctttccg accgtgctct tgccggtccc acggtatccg atcaacacga gattcatgcg
      661 aggatccatg ccctgacacg aggatgttgt gtggattcct gctgcgggag gccgaccgct
      721 gtcttaacca aagcgagact ccaagacccg tcgcatgaca tcggccgggg ccggctgttt
      781 cgtccagagt tcgaattgca acacggcctg gttgaggaac atctccaatc cggaaatcgt
      841 ccgacagccg gccgccttcg cttcctgcag cagcttcgtt tcgcgcggat tgtagacaat
      901 atccatgacc gtcagttcag gcctgaagag attggtcggc acacaggact cctccacccg
      961 aggcgacatc ccgaccggcg tgcagtgaat caaggtgcgc acgtgcggca cccagttccg
     1021 aagcatgtcg agcgtgagcg ggccatcctc aatcgggagg ttcgtcttct ctcgcaaatc
     1081 cttcaccaat ttctgccgct caacctcttc gatcccgaga atcgtcaacc cggcgatgcc
     1141 cgtcccggtt gccagcgcga aggcaatcgc tcgggctgcg cccccggagc ccaggatcaa
     1201 gacccgatgc ccgtcgagca acgccccgcc ttctttcaac gcgcgcaacg ccccggacgc
     1261 gtccgtgttg tagcccttga gcttgccctc ttccacgacg atggtattga tcgctccgat
     1321 atgcttggcg gtgggctcga cttcatcgag aaacggaatc gccgccactt tgtgaggaat
     1381 cgtcacgctg aatcctcgcg cattccccaa ggcccgcacc cctttcagcg catcgccgag
     1441 ggcttccacc cgaaacgcca ggtagaccag atccagcccc atcttctgaa aggccgcatt
     1501 gtgaatcgcc ggcgagaggg aatgttcgac cggattgccg atgatgccgc accattgagt
     1561 ctgagtcgtg atgtccatac gcaagaacct tctacttctt gagtgcgacg ttcagggcca
     1621 catactgaga tccttgcacc tgatacagca ccgccacatg atcgtctttc ttcagatcct
     1681 tcacggcctt cgccccaccg tcgaaatggg ttttttcatt ggcaacgaag gtaaaccggc
     1741 ttcctccgcc gtctttcttg acggcgaatt ttccggatgt cgtatccacc gacaagatcg
     1801 tgcccacgaa ttccgtccca ccttccgctc gcaccggctc cacgcttgat tctagcaatc
     1861 ctgacaagac cgcagccaag aagagagccg gaaccacaac tgcgctcata cgtgacatgc
     1921 cactcatgat cgcctccttg cttccggcaa ccaggtgcca cagcactctt cccgagtcgt
     1981 tgccctccgc tgcagcagag cggtcgcctt cttttgctta ccccttgctg atggtcatgc
     2041 ctccatcgat agggaacgtc gctccggtca cccaggtggc ctcgtcggat gcgaggtaga
     2101 gcgccatctt cgctacctct tccggcttgc caggccgacg gatggcgtac tgagccatga
     2161 tcggttccag gctggcagga tcggccatta aaggcgccgc catgggcgta tccaccagac
     2221 cgggattcat gacgttgcag cggatgttgt cctgcgcgta atcgaccgcg atggaccgtg
     2281 tcaacgagtc caagccgcct ttggacgcgg cataggccga cagaccgcgg atcccgacca
     2341 ggctcgccac ggtggaaatg ttgatgatcg aaccgccccc gcccttcaac agctcaggaa
     2401 gaactgcgcg ggtcatccga aagacccccg tgagattgat gtccagcacc ttcgcccagg
     2461 tggcatcgtc tatttcgtgg agccgtttgc cgaaatcgcc gattccagcg ttgttcacca
     2521 gaatatgcag cttgccgaag gtacgcaggg tctgcgcgac cacatcctgc acgtgggcct
     2581 catccgtgac ggatccgacc accgccaatg cccgccctcc gttcacaccg atccccttca
     2641 ccacccggtc gagctcttcc ttccggcgac cggttaccac gacggaggcg ccttcatccg
     2701 cgaatagctt cgcgatcgcc tccccgattc ccgcattccc gccggtaatg atggccactt
     2761 tcccctgtaa ccgattcatg cctctgcgtc ctccccctcg tgcgcttcgt catcctgctc
     2821 gccggctgcc tctgcctcga ccgttttgct cggacgcgac caccagccct cttcgacttt
     2881 tctggcgacg gtcaggaaac ccgaatgcgc caccatgcga tgatcgggcc ggacactccg
     2941 cccctgaatc gaccaggtcc gcagcaacgt ttcaaaggtt tcgatcatgc cgaataccga
     3001 cgtgcgctcc agcgcatcca cggtttgcat gacctgcggg atggtcggca caaaactcaa
     3061 ataaatgccg cccgagcgca agacttttgc cgcatgcggc accacctgcc agggttccgg
     3121 caaatccagc accagacgat cgaacaagcg gccgtcttcc agcacatcga tgccctcgta
     3181 ggcattcttg cgacagggca tgagggtaga cacgggcccc atatagcgtt cgatgttggt
     3241 caatgccgta cgggcaaaat cgtcacgggc ttcgtaggtg acgaccaggc cggtcggccc
     3301 tacggcgcgc aacaacgcca tggtgagcgc tccggacccg gttccggcct cgaagacccg
     3361 cgcgccggga tagacatccg cccacatcgg gatcaacgac aggtctttgg gatagagtac
     3421 ctgcgccccg cgaggcattt tcaggacata ttccccgaag gtcggccgga gcgcgaggaa
     3481 ccgtttcccc ttcgagaggg tcaccacggt gccgtccggc ttcccgatga gctcatcgtg
     3541 cggaatcgtt tcgccgctga agtgatagat atccccggct ttcaatgtca gggcatattg
     3601 ccggcccttt ttgtcgacca gatgaacacg ttcgccgttt tgtagttgtg acatagtccg
     3661 gcattctagg agactgccga cgggttttca accgaatcac cggtgacgcg cttccggcag
     3721 gccgcttcgc cgcgcctctt gacactgcga ggagcggccc tatctcgtcc catgggcaat
     3781 ccggattgcc cgctgtcgcg taataccact gagtgaatga gtcgagaaca gggacgaacg
     3841 acggtgaatc cttttcttac ccccctgtct ctaatgtagt agtggaacag gcagcctctc
     3901 caagctgtac cataagcgaa cctccccagg acggggaaga ggggtgacaa caatgaaaga
     3961 tgcgctcgcg attcaagacg atacagaccc gacggttgcg gctgaggtgg acccggatgc
     4021 cgatgacccg gaagccagca cgctgctgga cagcatcgtg caaggcaacc aggcggagac
     4081 cgaagcagac tccgaggact cggcgcctga gaagcctgcg cgctcgggct cttcgccctt
     4141 tctcctggaa tccctctact tccgttcgtt cggcgaacgc gcgctgctcg aacgggatga
     4201 agagattgcc ctggcgaaac aacttgatct cggcacacgc caaatccggc agacccttca
     4261 acaagcggtc aaagccggcg gccgcctgaa acgaaccgac cgcacgaccg agtgccttga
     4321 aacactcaaa acagtgcgcc gcctgagcgg tctgtcggcc accgtgctca atcaagcgga
     4381 acgcgcactg gaagaacttc aactggacgc caccaccggc ggcagagttc ccgtctcggt
     4441 ccgcagggaa ctggaggcgg ccttgacgca attgaaagcc tcgcgcatca ccctcgaaac
     4501 ggccaaagac gagctggtgc gctgcaacct tcgcctggtt gtgaatgtgg ccaaacatta
     4561 caccggacgc ggcctgaccc tgttggatct ggtccaggaa ggcaatatcg gattaatgaa
     4621 agccgctgag cggtaccagc accggaaggg gttcaagttc agtacctatg cgacgtggtg
     4681 gatccggcag ggcatcacca gatccctggc cgatcagtca cgcaccatca ggattcctgt
     4741 gcaccagacg gaagcttcga accgcattct cagggcctcg cgccgtctcg tccaacagtt
     4801 aggacgacag ccgcgcctgg aagaagtggc ctccaccatg cggatgcgcc ccgatcggct
     4861 gcatgagacc attcaggcct tccaggaacc ggtggcactc gagcatcgag tcggagatgg
     4921 cggcacggag ctgggcgaac tgctaccgga ccagcaggcg gtcccgccgg acgcccatgt
     4981 gaaccgcacg gagctcaccc gtgaaatgga tcgcattctc ggcaccttga cccccagaga
     5041 gcaaaccgtc atccggttac ggttcgggat cggggaagac caggcgcgta cactggaaca
     5101 ggtcgggcag catctgtcgg tcactcgcga acggattcgg cagatcgaag ccaaggccct
     5161 caagaagctc aagaccccga tggtcaagga gatgttcgcg gccatcaaat aaccacgacc
     5221 tgtttcatac ggcagcgcga tgcgggcgag gggtgacacc tcgcccgtgt tgtttgagcc
     5281 gcgctttcgc catgcatcct attgtgagag aatgcggcaa cggaggaacg gctctcccct
     5341 atgctgacag ccctctatag tcgcaacggc gcccggcctg atcggacacg atggttcgcc
     5401 tggggccttt tctcactctc cctcctcagc ctctgcacgc cggcgaacgg cgcaggccac
     5461 aatcccaatg aatcgacctc gctcacggaa ctgtcggtac ccgccgtcgt agccaaagtc
     5521 cgtccgtcgg tcgtcaccgt gctcacgcgt ggcattcccc agggaggctc ccaacacggg
     5581 gccccgccgg gctccggctc cggcgtgatc ctcgatgccg gcgggcacat cttgaccaat
     5641 aaccatctgg tgcagggcgt gaccagcgtc gtggtgggac tctcgaccgg ccggctcaca
     5701 ccggggcgag tcgtcgctcg tgattttctg ctcgatctcg ccctcgtcag aatcaccgct
     5761 ccggatctgg ttccggccga attcagccgg cgcgcgtcct tcgaaatcgg tgaaaccgtg
     5821 atcgccatcg ggaaccccct cgccctgaag ggaggctcca ccgtcacggt cggcgtcatc
     5881 agcgcactcg atcgctcggt gctcacgccg gagggagaga ccctgtacga tctgctccaa
     5941 acggatgccg cgatcaaccc ggggaacagc ggcggcccgt tggttgatcg atacggtcag
     6001 gtcgtgggca tcaatgtcgc aatcgctcct tccgcgcagg ccatcagtta cgccattgct
     6061 atggaggcgg tgacgcccca tcttcaatcc atgatggatc gcggctccgt cctgcggccg
     6121 gacctcggcc tgataccgct gacgatcacc ccgagtgtcg ccgcgagctt cgatctggag
     6181 agtgaccggg gcatcctcgc gctctcggtg gatccagcca aaccggctgg gcgagccgga
     6241 ttgcaatccg gggatgtggt gaccgccatc gataaccatc cgctgtacaa catcggagat
     6301 ttctggcacg ccgtgagtcg cgcaagcgac cagttgtcgt ttcagatcac cacgcaaggg
     6361 aaggccggca cgggcaccat tactgtgtca cgtcccggtc cggctcggcc gtaacccatg
     6421 aaactcgatc agcccagcca gacggaatcc gcgcacccgg tctcgaaggt gcctgcacag
     6481 gctcagccgc cccgagcggg catcctgata cgctgcgagc ccctccccta cgagatagcc
     6541 tggagtctgc agcgtgcctg ccggaccgag cgtgcggccg accgttgcga ggatctgctc
     6601 ctgctcctgg aacaccctcc cgtgtttacc gtgggccgca cgacgcaaga ccagcattgg
     6661 ccgggagaga gctgcctgac gcaggaatca ggcatccccg tgattcgcac ggagcgcggc
     6721 gggtcgatca cctaccacgg accgggccag ttgatcggct atcccgttct ccgcctttcc
     6781 gatttctgtg ccggaccgaa ggcctatgtg catcgattgg aagacgtgat catcagcacc
     6841 ctggccgagt ggggcattgc cggacatcgc cgggagcggc tgccgggagt gtgggtccga
     6901 ggggatgtcc cgtcgaaaat cgcctcgatc ggcgtgcgca tttcgcaagg cgtcacgacg
     6961 catggtttcg cgctgaatgt ctgcctggac ctggcgccgt tttctcacat cgtcccctgc
     7021 ggaattgctg actgccgagt gacgtctatg gcggcactct taggagaagc gcccgagatg
     7081 ggcgcagtcc aggaaaggct ggctgccaat tttgctgaac ggtttcatct ggcctgggca
     7141 gaacgcatcg ggccggatcg gtttttggct ctgagcgacg cctcaccgac gattgctcag
     7201 gggcctctcc ctctgcgcgc tactcaccag caaggagtta cgtgatgaac gaattcgata
     7261 cccacacctt tcggcttgcc gttgcccagt ttaatgaagc ggcagaatcc atgcgcctcg
     7321 acaccaacct gcgtgagcgc ttaaaactgc cgcaacgctc cctgctggtc agcataccgg
     7381 tgaagatgga cgacgggcat gtggaagtgt ttaccggata ccgtgtccag cacgattccg
     7441 cgcggggccc ctgcaagggc ggcatccggt atcatccgga cgtgaatctt ggcgaggtgg
     7501 ccgcgctggc catgtggatg acctggaaat gtgccgtggc ggatcttccc tatggcggcg
     7561 cgaaaggcgg cgtgaaggtg gatcccaaga aactgtcccg cggggaactg caacgcctga
     7621 ctcgccggta cgcggcggaa attttcccgc tgatcggccc ggacaaagat gttccggcac
     7681 ccgatgtggg cacggaccag caggtcatgg cctggattat ggatacctac agccagcagg
     7741 tcggctatgc cgtgcagggg gtcgtcaccg gcaagccgtt atcgatcggc ggcagcctgg
     7801 gccgggagga agcgaccggt cgcggcgtct cctacgtcac actggaagcc ttgcagcacc
     7861 tgaaactgga cgtatccaaa gcgaccgttg ccgttcaggg gttcggcaat gtcggatcga
     7921 ataccgccct catcatgcaa caagcgggcg cccgggtcgt ggcggtgagc gacgtcggcg
     7981 gcggattgta caacccgaag ggcctggaca ttgccgacgt gctccatcgc tatcgtgaca
     8041 aacatgaacc attgcgcgag atcaagctgg gcgagtcgat tagcaacgaa gaattgctgc
     8101 aactcgattg cacggtactg gttccagccg cgctctccga gcaaattacc cacgccaatg
     8161 catccaagtt gcgctgtaga attctggcgg aaggcgcgaa cgggccgacg acgctggagg
     8221 ccgatcgcat cctgaccgac aagggcgtgt ttatcattcc cgacattctg gcgaactcgg
     8281 gcggcgtgat cgtctcctat tttgaatggg tgcaggacgt gcagcgcttc ttctggaaag
     8341 ccaaagatat tcaagaccgg ctgcaagaca tcatcaccag tgccttccat cgaaccctgc
     8401 agttctcggt ggagaaacgg acgacgatgc gcatggccgc gttgatgtcg ggcatcgaca
     8461 aggtggccca ggcgcatctc caacgcgggc tctaccctta gacgacagga tgccgaagac
     8521 ctcgctcatg ccatgacacg gtacatactc gcggcgatcg ccggaatctg gatggccgac
     8581 ggcctcgcct tgctgacggt ccccctgctc gtcgtcaaaa gagtgcagga gtccctcctg
     8641 cacagcccac aactgctccg ctgggaagcc gtcggtatcg gcctcggagc cattctgatc
     8701 ctctggtctg ggccgattcc ctatcagccg ctgtggtgga tcaccggtgg cgccatgatc
     8761 atcaaggggt gcttcctcac atgggggccc gccgcatggc gcacgccgct gctggattgg
     8821 tgtttcgccc gcgaagccat cgactaccgg ttcttcgggc tgtggttgtg catgcttgcc
     8881 gttctgctgc tgcatgcgtt agggttgctc caccggtaac tacgcttaca gcgggcctcg
     8941 atcgagacca tcgtcatgag tcaccaaacc atcgcctcac tctgggaaga gcacagccgc
     9001 gagggctggc cgcgtttttc cagcccgaac gaaggacaat tgatgacgct ggataccgtc
     9061 atcgggggat gtgccgtgtt ttacctcgac gggcagacgg aaatagacag tcaacgcatc
     9121 gcgatactcc aggactgcgt cgccgacctg gataccttgc tcgatgacct gactgaagac
     9181 agcttgggct actttcaacg actccgccga ctcgcggccg ccctcgtcca gttgaatcaa
     9241 gcgacctaac cgcgacaccg cgaggcgcag cgggggccga ccggtaggcc tgcccccgct
     9301 gtctgttccc tacggcacct cgacgatatc cttcttcatc ggaccgagga tccccaacaa
     9361 ttcgcttttc tcaccggccg gcacgttaaa cttgtcgagc gcggcgacaa gatcctgaac
     9421 cagggctcca aaatccgcac tgctgatttg catccccagg tgcgtgcttt tcatgtcgcg
     9481 gccatgatag ctgcaggggc cacccgtcgc gccgcacact tgatcgacca ggtgcccctt
     9541 caacttgggg atgtccgtcg tagcaaagcg gctattaatc cgcccatccg ccgccacgtt
     9601 ggccacaaat tgatcgacca ccgccgtaat ggcaggcttt cctcccaaac ggtcatagag
     9661 ggactttgtg gccgtcgcac cggccgccgg gtgtccggct ccgttcaaac tcccggtatc
     9721 cgcgcaaccg gccagcaccg ccagtgcact gagtacaatc gctcccattc caacactttt
     9781 cgataccatc atccgccccc ccttgtaata tgtgaccgac tgctggccct cctcgaccca
     9841 ccatgagccg aggttttcaa ccagccgcac cggttgaccc cagacgatag gggagatatg
     9901 atctattcgt ctaatatatt gttttcatat gtcctatagc tttcccctat agcgagaagt
     9961 aaaagccatc atggaattgc gtcaaattga gtatttcatg gccgtggcgg cccaccaaaa
    10021 cttcagccgc gcggcggaac atgtccatgt ctcccaacct tctttatcga tccaaatcgg
    10081 cggcctcgaa aaggaactgg gcacccgcct gttcgaccgg ctcggccgga aagtcgtcct
    10141 gactcaggcc ggcgagttgt ttcatgtgca tgcggagcgg gctttgcgag agctggatca
    10201 ggccgagcag gtggtgcaag agctgctcgg cgcaaaacgg ggccgcttgg tggtgggcgc
    10261 actctcaacg gtcaactcct acctcattgc cccgctggtc tctcggttca agcaacgttt
    10321 ccccgacatc caccttcaag tgcatgccca ggcctcggca gatgtcgtga gtgggctgct
    10381 gtccaatcgc ctcgacatcg ggatctgctt actgccgctg acgcatccac aactgacgac
    10441 cgtcccgctg tttgaagaac gcctggccct cgtggccccg gcaggcatga acatcgggaa
    10501 acgtcgtgcg cgcatccaag acctcgcacg gcttcccctg gtcctcatgc cggtcgatta
    10561 ttgtctgcgc aaaatggttg aagccgaatg cgccaaggcg ggtgtgcatc cgcaggtggt
    10621 gctggaaatg agttcacccg aaggaatcct tcaggctgta gcggagggaa cgggagccac
    10681 gattcttccc gagctctacg tccgctcgcg actccccgga atggatctac aggtgatcga
    10741 tctctacgat cccactcccc gtcatgccgt cggcttggcg tatctcacga agcgacaccg
    10801 cggactggcc gctgaggagt ttgcagcgat ctgccacacg acgatgcggg acctgcaatc
    10861 cgatcccgaa cagtccaggc ggaaccggcc aacaaggcgt gcagcagggc agaaatagca
    10921 tcccctctat ccgctcacct aaccgctacc gctctaactg aaagaccggc cgcgaccttg
    10981 aaagtcagac tgccccttgg atagaatgcc atgttctctg agtcatcccc ccgaaatcct
    11041 ataccaactg tcagaacggt gtgagttctg aggatctgtt ccaaggaggc cttcatgatg
    11101 cagcgcaagt gggcgttcac agccttagtg gcgatgatgg gatcccttta cctcgctgcc
    11161 atggtgagcg ccgggcctta tgaactcagc aaggacgact tgaccgaccc caaggggatt
    11221 gcgagcccgg acgtgtccct cttcggaatc aagttggggg atgatgaagg aaaagcgctg
    11281 gaggggctgg tgaacgaaaa gatccccggc atcaaggccg aacaggaagc gacgtttatt
    11341 ttcctgcttg atcaacgcaa accgaccggc ccgatggccg gggtccgggt acaggacgga
    11401 aaagtcgatc tcattttcat caacaaccgc tttgcctaca aaactcgtgg gatctttcgc
    11461 aacgtcctca acagtgagag tccggatgat gtgcgcaagt tgctcggcaa agaagattac
    11521 ggtgatgaaa acgtcatggg cgcaatactc gcctatgaca agcagggctt ccaggtcaac
    11581 tatctcggaa aagatgtgaa cgtggagttt tcgctccccc actaagacag cccggtcgtc
    11641 tgttcagttc cgccgtactt gtcccctcaa cacctcaaac catgaagacc tgtgcccttc
    11701 ttccgatggg cacaacctgg ccccgcttgc cacagcaaga ctgaccgtcg cgcaccatcc
    11761 cgggaaaaac tagacgtcgt taacgggcaa gaccagcccg gctgtcactg agccgcaggc
    11821 cgtagttgat gaggctcgtc gccgtgttcc agcgacgctt ggaattgaga atgaccagca
    11881 gcaagtcgct gccgtcttgg gagactttcg cgatgaggca tcggccggct ttggacgtga
    11941 agcccgtctt gacgccctgc actcccggaa tacgacccag gagacgattg gtcgtgcgca
    12001 acacgtaggc ccgatgctca ttgatcggca tgatgatctc ccgctcttca cgcaccaatt
    12061 ccttaaatac gggatggtgc agggcgatct cgctcagttt cgccagatct tccgccgtcg
    12121 aataatgttc cggcgcatcg aaaccgcacg cgttgctgaa atgggtattg tgtaactgaa
    12181 gcgccgcggc cttggcattc atgagatcga caaacctggc ctcatccccg ccgacatgct
    12241 cactggccgc caggcaagcg tcattcgcag acacgatcaa catggccttc aacaaatctt
    12301 ccagccgaaa aatctgaccg gtccgcaagc gcaagtgcgt cttgtgtgcg cgcgcggcct
    12361 tggggctgac cgtcacctcg tcatccagat ggccatactc caggatgacc agggccgaca
    12421 tgatcttggt cagactggcc ggcgacaacc gcttttcact ttcaaattgg tagagggtgg
    12481 ttcccgtctt gagttccttg agcagaatgc tatgcgccgg aacatgccgc caccggagac
    12541 tatgattcgc ttgcttggcg gacggaaccg gccgcggatc gaagggcacc gtaatgactt
    12601 catcgtctac gtcgctgtcg agggccagcg cctgaccgct ccagaggccg aacaacagag
    12661 ccaggattcc gaaggcaccg atcgaaaggg ttcgacgtaa acgattcatg gagagagtcg
    12721 cactttcttg tgacgcacag acgaagtctg acgttcacag ggtataggtt tctcgcacct
    12781 tcgtaccacg caggctactg gagatcacct tatgaaagaa tcggagcaaa tcggcgagat
    12841 cgatccagaa tccgcaattc aggcaacggg catagagacg ccgattcatg agcgtgtaat
    12901 gccgctccac catcatgaat cctttgcatt tcaggcagtg catcgcccaa cccttgcccc
    12961 ttcgaatcgg tttcgtggtc ggcccagcgc agctcaggcc gataccgacg tcgtcactgt
    13021 atttagcgga gtcgattgag gatcagggcg aggcagccgg ccaacaaccc gcccccgaag
    13081 actgtggtgc taaaggagag gatcgcgccc gaacttcccg ccgggttggt ccctgagacc
    13141 agatcgcgca ccccaccttg aaccatcaag actgccagcg tcgtcaggca gcccatgcaa
    13201 aaccaccaca gaaacgatcg cacgagcccc cgcggtctag tagctgaaca tggcgcggat
    13261 ccttgtccgg caaccttttc gaactctatc cgagccctat tgaggtgtca agcaaactgc
    13321 ctatctcctc ttcggatgaa ccggcgcaaa cctgaatcct gctgctattc gtccgcgcga
    13381 ccggccgccg aagcttgccc ctttcgcccg tgatgcagga acaccgacac actacccgcg
    13441 ccgttatccg ccgtcaccag accaggctct tccatgttat cggcagagac acggtaactc
    13501 gccacggcaa agggtccgtt cctcgtacgg taattgcgag gcggataatg aaacgtgccg
    13561 tcgcctcgcc cgaagagaat ggagagatcg gtcgattgca gattcacgat ggccacatcc
    13621 gcaatatggt cgccgtcaaa atcgtgagcc gccccgaaat tcgggctggc atcggcgccg
    13681 gaatccttac cgggctggaa ggtggcatcc ccattgccca acaatgtggt gaagctgtcc
    13741 ccttctccgt tgatgactaa cagatcaggc agagaatcgt tgttaaaatc cgcgaagctg
    13801 acgccaagcg gccggcgccc ggtcttatag tctttgggat cccgaaatgt cccatcgcca
    13861 ttgccgatcc acacagatac ggcacttaac atcggtccgc cattcgtgac caccaaatcc
    13921 ggtttgccgt cctggttgag atctttcaat gccaccgacg tgggtgtgtc gccgtattca
    13981 tattgaaagc cgggacgaaa ggttccgccg gcactcccga gaaaaatctt cactttgtcg
    14041 ttgcgcagcg ccaccgccag atcgggatga ccgtcaccgt tcacatcctc gctggacaca
    14101 gagaccggcg tacgatggac cggatactgc ggcccatctt cgaacttccc gttgcctcgt
    14161 ccgaacagga tcgagacctg atcactcccc gagcaggcaa gcaccacatc gagttggtca
    14221 tcgtgattaa agtcgttgat ggccagggag cggggctcct gacacacctt cacctgaacc
    14281 tgatctcgaa acgtgccgtc gccgttgccg aacaacagcg aaatcgtatt gctcgatatg
    14341 ttggtggtga taaggtccgt gatttggtcg cgattgaagt cggctgtggt gacggtggtc
    14401 ggattcttac cgaccgggta actggcaaag tagtagaaag ggtcgggcgg aacgtaggga
    14461 tcggccttcg aacaggcgga aagcatgatc aacccggcca ataacaccgg tccaacaccg
    14521 aagaacgacc gaatcttgat gccccgacgg tcggcggtca tatccatctc gatgaaaaag
    14581 gcatgacatt ccggccaagt ccatgcttcg ccgctcccgg ttctcgtccg ttgggcggag
    14641 tatacagagg cccctcgggc ttcgcaacga atggcccgct ccgagaacat gccgccgggg
    14701 cgaacacgac tacttgccga atgggctcaa gcatctaggc ctttcgcccc tttgaaaact
    14761 tggaaagcct taggctacaa tgcgggccaa gtgtggagga ggtgcccatg agcaagacag
    14821 ccaatattcc tttgtcgatg tacggtgtgg cagaagtgtt gaagtggtgt atcgatcgca
    14881 ataacggccg cgtcagcggt gtcgatacgg acggatttaa gaaaatgcag gcgctcctgg
    14941 cggagaagcc gacgtcgaat gattacttgg cactggacca gttctggaag aagcaagtca
    15001 tgttggaact gacggaagac gaggtatcga ccatcgaccg ctgtctttac gatattccaa
    15061 actttgacaa cgaaccgctc ccgcagattc gacataagtt ctggcctgac gccgtcggag
    15121 ctcactaagc cccggctatc cgcgatcgac caccatcttc cggcaaccca cagatcgtca
    15181 tgcgctgtgg gttgctgtgg gattgtgctg tttgagatca cgcgcccaac gctccactca
    15241 taccttccct cccgaaggag actgtgcgta gtcgcggcca tcctgcgata gcctcaagta
    15301 tgttgatttg attgttggcg ggggaatagg cccgtccagc cacgcgatga ggcagaacgg
    15361 gccccggatc gacagggcct gggaaacccg aacagattga tgttcctgct ttagagcgac
    15421 ccctctcctt tgagaaactt ccggaatcga cccatgaggt cggcgccaag ggtgccgcct
    15481 tccggtttct taacctccgg gtcactgaag tgtgaccgga tttcctgaag gcgcttctcc
    15541 gcctgatcgt tcccctgcaa tcgcttggtt ttttggaggg cggtgtcgaa ggcatcgaac
    15601 gtgagttcgt tatagccgaa cgaccgaatg cgcttcacag ccttcatcat ggcctgattg
    15661 cggaatgtcg tgaggtgctc ggaatcgacc tctttcaacc ccaacttgcg ggctcgacgt
    15721 tcggctgcct tgcggatccc gctgcgcacg aaatccggtg aggtttgcaa ccgcttccag
    15781 gcctcatcgc tccaggcgac acgttcttgc ggggcctccc acaactgcac cgcagtctgc
    15841 tcatcgattc tggtgaaccc cttctctcgc gccagctctt ccgtatcatg ccggatcatg
    15901 tcgctcacga aatccatggc aatcggcggg gactccttga tcttctgctg caagagcgcc
    15961 aacgcgcctt cggaccattc cagtggagcc gcctcccgct cgcggatctc acccaaggcc
    16021 tcgaccttct caaagagatc gacattgacc tccatgtggc cgcctttgcg ggcgatctcg
    16081 tcggcgattt gcttgatcat cccgcgcatg aactccggca cggccgccag gcgttccttc
    16141 gcggccgcgg tccaaggcag ctgtcccttg gccatttgat cggcggcctc agccatgccg
    16201 ccctcaccgc ccatggtccc catcatctga cctttgaact gatcgaggat ttcttcggtg
    16261 atttcgggat agcccaactc tcgcgccttc ttctcagcca ggcggcggac cattcctcgc
    16321 aaaaacatgg gagcgcgctc cattcgcttg agcgcgccgt cactccaccg aacctctggg
    16381 gctgttatat ttgaggactc tgccacggta tcactcctgt tacttattga gtaattcctt
    16441 cagttccttg cctgccttga agaatggcac acgcttggcc ggcaccgcaa cggtcgcacc
    16501 ggttttcgga ttacgacctt ccttcatccg ccgggcgcgg agacgaaaac tcccgaaccc
    16561 tcggatctcc gtcttattcc cgttccgcag cgagtcgcga atacaatcga atatcgtgtt
    16621 caccacgatc tccgcctgcc gcttcgtcaa tgtcgtgacc tgctccgata cccgctcgat
    16681 aatctgcgct ttggtcatcg tccctctcct cgtgggtggg gcttacgcac ggatgaattg
    16741 ctgcgttgcc ttaccgatgc gctccggccg ttcagaacgc catcaggtat ttcagtttga
    16801 cgccggtatt caactccagc ttcggaaaca ccgacgccca acgtgactcg atgatatccc
    16861 gaaatgaaaa ccgcttgcga ggctcgacca ctttcggctc accctccata ccggcaatgt
    16921 ccgccgcgat gtggatggca tcgtccagat cgcccagttc gtcgaccagc ctcgcctctt
    16981 tggcctggcg tccggtgtaa atccgtccat ccgccagcgg ctccacatcc gacacctcca
    17041 acgaccgccc gtcggccacc gcttgaatga actggtggtg gacatcgtcc atcaccgact
    17101 gcaggagctt gcgttcctca tcgctcatct tccgcaacgg ggacccgacg tctttgaatc
    17161 gcccgctctt gatgaccacc ccttcgaccc cgaccttttt catcaggcct tcgaagttgg
    17221 ccatttccat gatcacgccg atactcccgg tcaacgtgcc gggattggca atgatacggt
    17281 ccgtggccgc ggcgatgtaa taacctccgg aagcagcaac cgtccccatg gaggcgatca
    17341 ccgccttatt gctcttgttt tttacccgct tcaccgcatc gtgaatttcc tgagacggca
    17401 cgacgccgcc gccgggactg tcgatgcgca gtacgatcgc cttgaccagc gggttctcgc
    17461 tgtattgctt caactcgctg atggtcgctt gtgcatcaag gatcacgccc tcgatgcgaa
    17521 tgagggctac acgatcctgg cttgagaaat cgaggtctgg gaggagcgcg ttcaagagaa
    17581 tcaacgctcc tcccccgatc acgatggccc agaagatacg tcgcagcagg ctccgctgtg
    17641 gtctcaccgc cgtgtctgct tgtggcccca ttcggtctcc cgtcacctag gcttcggaat
    17701 cgctctggtt ccgcttccgg ctttgcttgg ccgcgcgacc cagggtctga tcgatcccgc
    17761 cctgagaggc gtggaactca tcgacctggc gccgatcgga atccatctgg tgatcgcgca
    17821 agctgagcgc aattttccgc tcatcgcgat ccaccttgat gatcttcgcc gtgacgtcgt
    17881 cacccggctt gaacttctct tccatgcgaa cgttcgtatc gagtccgact tcgctgatat
    17941 gaatcagacc ctcgacatcg ccatcgagct cgatgaagag tccgaaatcg gcgatcttgc
    18001 tgaccttgcc ggtaatgcta tcgccgaccc gatacttgtt gggaatctgc tcttcccacg
    18061 gatcgcgtga caattgctta taccccagcg agaggcgctc cttttccttg tcgatacgaa
    18121 tgaccaccgc atccaccttc tggcccttct tgaagagctc cgacggatgc ttgatgtgct
    18181 tcgtccagga catgtcggaa atatggatca gcccgtcgat accttcttcc aatccgacga
    18241 acgcgccgaa atcggtcagg ctcttcacct ttccttcgat ccgcgttcca gccgggtact
    18301 tcgcctcgat catgtcccac ggattcggcg ccgtctgctt catgccgagc gaaatcttcc
    18361 ggcttcccgg atcgatgttc aacacggcgg cttccacctg atcgccgacc gagacgacgc
    18421 gcgacggatg tcgcacttcg tgcgtccagg acatctcgga cacgtggacc aatccttcca
    18481 cgccgggctc caactcaacg aaggcgccat agtcggtcaa gctcaccacc cgaccacgga
    18541 cgcgcgtgcc caccgggtac tttgccgcca caccggtcca gggatctgcc gacttctgct
    18601 tgaggcccaa ggaaatacgg ccggtttcac gatcgtactt cagcacggtg acttcgacct
    18661 tgtcgctgac ctggaacaat tcggagggat gccccacacg accccaggac atatccgtga
    18721 tgtgcagcaa tccgtcgatg ccgccgaggt cgataaacgc tccgtaatcg gtgatgttct
    18781 tcaccgtccc ctgaatcaac tgaccttcct tcaaggtcga caacgtcgtc tgacggcgac
    18841 ggtcgcgcgt ctcttccaac aagacccgtc gggaaacgac cacattgcca cggcgatggt
    18901 tgattttgat gatcttgagg gggaatgtct tcccgaccag cccatcgaga tcgcggaccg
    18961 gatgcagatc gatctgggag ccgggcaaga acgccttgac gccgatatcg accatcatgc
    19021 cgcccttgat acgggaaatg atcttgcctt cgatgctctt ttcttcttta tgcagcgtct
    19081 ccaattcctc ccagattttc atcttgtcgg ctttttcttt ggagagcacg agattgccgt
    19141 ccgcgtcttc acactcttcg agatagacct ggaggcgatc cccgaccttc aactgggcca
    19201 attcttcatg cgagaactgg tcggctggaa tcatgccttc cgacttgtag ccgatatcga
    19261 ccacgacttt gtccttgccg atggccacga ccatgccctc ggtgatggtg ccttcctcaa
    19321 aattgcggaa ggtttcctca tacatcgccg ccaaggcgtc gcgatccagc ttgggttcac
    19381 tttgcggtgt gacagtactc atgaaaaggt cctcgtcctc cggctcgtgc cgggtgctga
    19441 tagtgagaaa aaccgagtct tactcagcgt tgcaagactt ggctgttggt gttgcggcgg
    19501 aatcgtgggg ctgcccggcg ttggccggtc tgtcccccgg aatcggaacc tgtccgagct
    19561 ctgcgatctt cgccatcacc gtccgactga catgttgata aaacgctttt ccttccaacc
    19621 gcgcggcatc cgcggaaaaa tccagcggct ctccgtaggc caccgtcacg cgacgaaatc
    19681 tcggccactt ggcgccgggc ggcagcacct cgaacgtgcc gccgatatgc gccggcacaa
    19741 cctgacagcc cgtctgcgac acgatcaccc cgatccccgg cttgccgggc ttcagcgcgc
    19801 cggtcatcgt acgcccacct tcgggaaaaa tggcgacggc cttcccctcc tgtatcaacg
    19861 agaccgcttt gctgaaggcg tcgcgatcga gacgaccgac ccgtaatgga atccacccca
    19921 gggcctgcag caggccgttg agcaacggca tcggaaacaa atcgtgccgc cccatatacc
    19981 agacccggcg gggaatcccg cacccgagca gcgggatatc cagataactg gcatgattcg
    20041 aggcgatcag gaacccgcct tgccgcggca cagtcccgac ggtgcgatac cggaagcaca
    20101 gccacccgat ggtgcgagaa atgacccaca acaggccata cagtgccgag ctcacaatct
    20161 cgccgtgatc acggccagca tgtgctcaac gactgcctcc gccggcatac tcgacgtatc
    20221 gatccgttca gcatcgggcg caggaatcag cggcgccacg gttcgagaac gatcgcgatc
    20281 atcccgcccg gtcatatcgc gtttcgtctg atcgaattga accgaatgac ccgccgccac
    20341 taactcacga tgccgtcgcg tcgcccggac ctccgcatcg gcctcgagga aaaacttcac
    20401 atcggcttcc ggaaacaccc gggttccgat gtcgcgcccc tcggccacaa cgcacccctc
    20461 ggcgccgatc tgccgttgca ccggcagcag ccattcgcgg acagccggaa tcgccgaaac
    20521 catggacgcc agcgccgtca ccgcgggcgt gcgcaattca ccggtgatgt cgcgaccatc
    20581 cagcaacacg tgcgattgct cgggaccaca ggccatatgc agcgtcgtct tgggaagcag
    20641 cgcggtgatg gcgaactgat cctcagggct caatcccgcg tctcgcactt tccacgcaat
    20701 ggcgcgatac aaggcgcctg tatccaaata gaggtatcca agccgcaagg ccagaagtct
    20761 ggcgaccgta ctcttgccga cccctgccgg accatcaatg gcgatgatca acccgcgctt
    20821 cccgcagccg gatgtgcccg gttcacccac gtaatcctcg cactatagac gtttcccaga
    20881 atcagtcaac agttccaaga gcttacgatc gaagttcggg aacgaagtct cgatgcaaac
    20941 cgtgccctga atctgagtcg gctgggcagc ggtcagggca ccgatcgcga cggacatggc
    21001 cacgcggtgg tcgccgtgac tcgcgcaggt cgcccctgtg agcgcgccgt tcgcaccctt
    21061 gcgccccagc ccctgtatca ccatgccatc cggccgttct tcgatccgcg cgcccatcgc
    21121 ccggagttca gccgccatcg tcgcgatacg gtcgctttct ttgactcgca gctcttcagc
    21181 tccggtgatt actgtttcac cgtcggccac ggccgccgcg acacacaaaa tcggaaactc
    21241 atcgatcgtc tgcggaatct gttccggccc gatctgaact ccccgcagcg gcatcgaccg
    21301 tacgcgcagg tccgcgaccg gctccccggc ctcttcacga ggattgagca cctcgatgtg
    21361 cgcgcccatc tggcgcagaa tgtctaatag accggtccgg gttggattca tgccgaccga
    21421 cagcacggtc acatcggaat ccggcacaat cgaggcgccg acgataaaaa atgcggctgc
    21481 ggacaggtca cccggcacca ccacggtttt tccactccag cgtatggagg gccgcccttc
    21541 gatccgaacc gtacacccgt cacggtgaaa aggaattccg aagtaggcaa acatacgctc
    21601 ggtatggtca cgggaaagcc gtggctctga aatggtcgtc aggccatcgg catacagcga
    21661 ggcgaagagc agcgacgatt tgatctgagc gctcgcgacc ggggactcgt acgacatgcc
    21721 tttcagccgg gtcccggtga tcgccaaagg cgccagttcc ccgccctttc gtccggcaat
    21781 cgtcgccccc atggctcgca agggtttcac gacacgtccc atgggacgac gtctgatcga
    21841 ttcgtctccg gtcaggaccg tgaagaaatc ctggcctgcc aacaaccccg ccatcagacg
    21901 aatgccggtt ccggaattgc cgcaatcgat cgggccgaac ggctccgtga gaccccacat
    21961 gcctttgcca tggaccgtca accgctccgg cgtctcttcg attcgcacgc ccagcgactg
    22021 gaacgcgcgc atggtattca aacaatcttc cccgcgacaa tagtcggtga cctggctcag
    22081 cccctcggcg agggccgtca ggataatcgc ccgatgggta acggacttgt cgccaggaac
    22141 cgcaatcgtg cccttcagcg gacggcccgg cgtaatagtt aaggatgcca tggcagccta
    22201 gctcttcccc gatgtgcgag gggtgagttg ttcccgttca tacttggcct gatccagttg
    22261 cttctcgatc ccagcggcgt caccggccgc aatcaaccgt ttgagttcgc cgagatgccg
    22321 ctcgtacgtc tcgatcaacg acaccacatt gtcccggttc cacaaaaaaa tgtctcgcca
    22381 catctccggg gaactggccg cgattcgcgt ggtatcgcgt aatccgccgc cggaatggct
    22441 ggccagatcg agttccggca aaccatggtc acgcacgtcg gccagcgccg tcatcaatgc
    22501 aaacgcggcg acatgcggca ggtgactgac ggcaccgaga atcttgtcgt gcagaaaggg
    22561 atccatatcc aacacaatcg aacccgcgag ttcccagaga acacgaaccg tttgaagcgc
    22621 ctccggatcg gtcttggccg tcggagtcag aatgcaacgc gcccctgaga aaagagtctc
    22681 tgatcctgcg gccaccccgg tcttttcctt gccggcaatg gggtgcgcac cgacgaaccg
    22741 caccgacggc ggcagcaagg cctccgcgcg tgtcaccagc tcacccttga cgctgcccac
    22801 gtcactgaca atcgcgcccg gcttcaggca accggcccat tcccgcaaat gccgttcgta
    22861 ggtatccacc ggagtcgcga gcaggacaaa gtccgcccct tcgacgcctt cgcgggggtc
    22921 cgatacatac cgatcgatgg cgcccacttc gacggctgtc ttgagatttt caacgcgtcg
    22981 cccgataccg acaacggaat ccgccagctt ttgacggcgc aggatcatcc ccagcgagcc
    23041 gccgatcagc ccgactccga caatcgccac ctgtttgaaa tgtggtgtca tcattgaatg
    23101 agtgcgtatc ttcaactcac ccgcacggtc gacgatcacg ttcgtaacgg tgatcagatc
    23161 gtccggtcga ctgccgcagc aattctccgc aaatccccca tcaactcctt gaacttcgag
    23221 ggccggatag actcttcgcc atcgcagagg gcacattctg gattggaatg cacttcgatt
    23281 aaaagtccgt cggcgccggc tgcgatcgcc gctttcgaca tgggcgccac caaatcccat
    23341 ttgcccgtcg catgactggg atccacgatg accggcaaat gcgacagact cttgagggtc
    23401 ggaatggcgg ccatatccaa ggtatttcga tactgcgttt caaacgtgcg gatgccgcgc
    23461 tcgcataaca tgacattccg gttgccgcgg gacatgatgt attcagcgga gaggaggaat
    23521 tccttgatcg tggccgagag accgcgcttc aacagcaccg gcttatcgta ggccccgact
    23581 tccttgagca actcgaagtt ctgcatgttc cgggcgccga tctggatgat gtcggccttc
    23641 tcaagaaaca gctcgatatc gcgggtgtcc aggatctcgc tcaccaccgg caggccggtc
    23701 tgttttttgg cttcgaccaa atagtccagg ccttcacgcc ccaatccttg aaaagaatag
    23761 ggcgaggtgc gcggtttgta tgcaccgcca cgaaggacgg tcgcgccggc cgacttgacc
    23821 tcgtgggcaa taccgacggt cagttccaac cgttcgaccg cgcagggacc ggccatgatc
    23881 gtgattttct tatcgccgat tttgacgccg ttcacgtcaa tgatcgtgtt ctctttcttg
    23941 aactcccggc tgaccagctt ccagggagcc aaaatcggca ggacactttc gaccccgggc
    24001 aatgcagtca acggctgatt gtgcaggata cgatcgtccc cgatcacccc gataatggtc
    24061 cgctcctggc cggcagaaat atgcgacttc agtcccaact cgcgaagccg gtcgataatg
    24121 tgatcgactt cccgctccga cgcgtcaggc ttcaagacaa taatcatgat ctcgtctcgc
    24181 tttccttatc gagcggcgcg cgtcacgtcc gcaagcgcgc tcaggaacag ttgattttct
    24241 tccggaaggc cgatggtcac ccgcagcatt cggccgtcga tgtggcggac gatgatgccc
    24301 ttccgcaaca gcgcgtcgaa tacctcgcga ccatctctcc cgacatcgaa atagagaaaa
    24361 ttcgtttcac tgggcaaggc ctcgaagccc aaggtcaaca agcctgtccg gaccttgtcc
    24421 atttccgcat ggttcaaggc ccgactcgcg cccacatgtg cctcatcgtc caacgccgcc
    24481 aacgcggcgc gttgtgccat gctattcgca ttgaagggtg gacggattcg attcaggtag
    24541 ttcgtgattt ccggcgtggt catcccatac ccgatcctga gcccggccaa gccgtagatc
    24601 ttggagaatg tccgcagcac gatgacattg cgacccgcgt ttacatagct gagcgactcc
    24661 gggaattccg gatgccggac atattcgtaa taggcctcgt cgaacaccac caccacatga
    24721 tcgggcacgc gcgccatcaa cgccgccacc tcagccttgg tcgccatggt gccggtggga
    24781 ttgttcggat tgcacacaaa cagcaggcgc gtcttgtcgg tgatggccgc tgccatggcc
    24841 ggcaaatcgt ggtgccaatt cttctgcggc acttccaccg ccacaccgtg cgcggccttc
    24901 acctccatct tgtaaatgac gaaggtgtgc tcagccatca cggcctcatc acccggcgac
    24961 aaaaaggttc gcgcaagcag gccgaggatt tcgtccgagc cgtttccgag aatgacgtga
    25021 tccggcgtga ctttccaccg ttcggccaag gctccgcgca aacgaaacgc cccgccgtcc
    25081 gggtaccgat gcagagtcgg agcggtttca cccaagacgg cgagcgcttt gggcgacggg
    25141 ccgatcgggt tttcattgga tgccaatttg atcgcgcgcg ggagccctaa ctctcgctgc
    25201 agctcttcga taggctttcc aggaacgtac gggacaaggg aggcgatatc gggatgcacc
    25261 ttcaatggca tggcgtgact cggacctcta ggaataggcg ggatacgacc cgagaatctt
    25321 cataaagaga cagcggctct tcacctcttc ggccgccttc ttgacccgtt cttcatcgat
    25381 atgcccctcg atgtcgacaa agaaaatgta ttcccacgcc ttccggcggg aggggcgcga
    25441 ttcgatcttg gtcatgttga ggccatggga ggcaaacgga cgcaacaagt cgtatagcgc
    25501 acccacctta tctttgaccg acaacatcag cgacgtttta tcgcgcccgg ttcgctccgg
    25561 cgccttctgc gacaacacca gaaaacgcgt gaagttgttg atgttgtctt cgatccgggc
    25621 cgtgatgacc ttcagtccgt acaactggga cgccagttcg gacgcgatgg ctgcagccga
    25681 cggatcgtcg acggaaagtt cagccgcccg tgccgtactg gccacttcgg acaccggcac
    25741 gtgaggcaaa ttcgtttcca accaattgcg acattgagca atggcatgcg ggtgcgaata
    25801 gatccgcttg atctctcctg cgaccccgct cttggacatc aggtgatgcg ccacttcctg
    25861 caacacctct ccatagatga gcagactgga atcgacgaac atatccagcg tgtgattgac
    25921 tacgccttcg gtggtgtttt cgatgggcac gacgccaaaa tgggctcgac cacgctcgac
    25981 ttcgctgaac acatctttga tgctgttgac aggaatgtat tgggccgacg acccgaactt
    26041 ctgcatgcag gccatgtgcg taaatgtcgc tcgcgggccg agataggcga ccttctgagg
    26101 cccctcaagg gacaacgagg ccgacatgat ctctcgatac accgcccgga tggcatcggt
    26161 gggaaaggga ccggtatttt gcttgctgag gcgttcaaaa atcgccgcct ctcgggccgg
    26221 cgtgtgcaga tgcgcttccg catctctctg tttcttgagt tttccaattt cgatcacgga
    26281 cttcgagcgc tcgttcaaga ggcgcaaaat ctgatcgtcg atccgatcga tttcctggcg
    26341 atgtccttgc aggtcatcag acatgacggg gggactccct cctcgcgaca cctgccttta
    26401 gagagacgag gccgcgaaac gattccaagc caacgggatg aacttggaca agtgagtatc
    26461 ctacaggagc cggcgagggc attgcaagga taggtgtggg gattcaagga gttgtgacgg
    26521 atgtgtcgtc atcggagagc gacagaaggt ctttctgttt tttgaaatgg tcgaaggcca
    26581 gcctggtcgc ctgccggccg cgaccggtgc gttccagaaa cccggcttgg atcagatagg
    26641 gctcatagac gtcctccagc gtgccccggt cctcctgcac cgccgccgct aaagaatcca
    26701 ccccgaccgg tccgccgttg aacttctcca tgacggtcag gagaatgcgc cggtccatct
    26761 catccaatcc ggcggcatcg actgccaacc agaccaacgc gtcctgcgcc acctgcttgg
    26821 tgatacgtcc accggccttg atctcggcat agtccctgat acgtttaatc agccgattca
    26881 cgatacgcgg tgtgccgcga gcccggcgag caatttccgc agcacctgcc tcgtcgatcg
    26941 gaatattcag cagccctgcc gaccgtgtaa cgatcgacgt aagttcctgt gacgaataaa
    27001 actccagcct gtgaaccaaa ccgaaccggt cccgcaacgg cgacgtcaat gctccggccc
    27061 tggtcgtggc tccgaccagc gtaaaccgcg gcaattccag cttcacggtt ctcgtggagg
    27121 ccccttgacc gaccaccaga tccagttggt agtcctccat ggccgggtac agggcctcct
    27181 cgacagaggc cgggaggcgg tggatttcgt cgatgaacaa cacatcccgt tcctgcaaat
    27241 tcgtcagaat cgcagccaga tccccggcat ggctcaggac taagcccgag gtcgaccgaa
    27301 tcgctgagcc catctcccgc gcaatgatat gcgcaatcgt ggtctttccg agccccggcg
    27361 gcccgtaaaa aatggcatgg tccagcgcct caccacggcg tttggccgcc tcgatacaga
    27421 tctccaggga ttccttcatc cgtgattggc cgacatactc ctgcaagctg tgcgggcgca
    27481 gcgcggcttc aaggccacgt tcgtcgtcgg tcaattgatt gcttaccgtg cggtcagaca
    27541 tgggacgatc ctgttcgaca atgcgtcggc gttatctggc ctcgggtgag ggcctgaatt
    27601 tcggcggcgg cgacacggcc ccttgacctg gcgcagccgt actgccgcaa tcaggcgggc
    27661 acctgactcc accctgaccg ggatcgggca tggttgatcc catgttctgt aactggcatt
    27721 gcgacaccag cccaccatct acagagccgc aacgcgaggg gacgttcgag cgccccactt
    27781 ctacatactc ttcgggacag gtgccgcaga cggagagcat gttgcccccg agcggcacgc
    27841 actgcaccag ggtcggctcg ccgtccttgc acagctccgg cgcatgggtc acaccggttg
    27901 tggcatagcc ctccgggcac attccgcaag tcttgcggat gctcttcggt tcctccacac
    27961 catcggccgc gccgactaac ccggcggatc cggacgagac catcgcggcc ataatgacca
    28021 gcctcaccgt accgactaac cggcgactac cccaccacct gctcaacgac ttcatcatgc
    28081 gtcacccctt ggccaggtct ttgagtgcct cacgaatcac gccttccagt ccctgcgcca
    28141 tagcgccgga cttttcgatc ctcttcaacg cttctttcac atccggttgt cgatagccca
    28201 aattcacaag ggccgacagg gcgtcctcat agagccgatt cgcagatcgt ccgctccccg
    28261 gctcggccgg ttcgcccgct ggatgcaacc gcgcgacctt gtccttcaat tccagcgcga
    28321 tcctcgccgc agactttttc ccgatcccgg acactgtgcc taacttctca atatcaccga
    28381 cctggatggc ggaaaagaga tcgcggactg agagggtgga caggacactg aggcccaatt
    28441 ttggtccgat gccggagatc cccgtcagca acagaaaggc ttctttctcc gcggcggtca
    28501 gaaatccata gagttgaatc gcatcctcac gcacatgggt atggatgctg agcgtgacaa
    28561 gctcatgctg attagggaga gaatagtagg tactgagggg aatcaatact tcgtagccga
    28621 cgccatgcac atcgagcgta acttgcgaag gcgccttgaa ggccatgagg cccgtcagta
    28681 aggcgatcat gtgatcaaca ggggggtgtg atgcgaaggg cccgagaggc tggctgcgtg
    28741 cgcgtcgatg ctcggtgcca atccttcaga atcaggcggt agcggcagcc accttctcca
    28801 tgacctcgtc ggagatgtca aagttcgcat agaccttctg cacatcgtcg tgctcatcca
    28861 gaatttccat gagcttcaac atttgctcag cagccttctc ctccagcgcg atcgtattct
    28921 gcgggatgaa gttcaattcg gcaagcgtgc actcgatttt cgcatcgctg agcgcttttt
    28981 tgaccgcctc aaaatcgtgg gtgtcggtga ggacttcaaa ggccttgtcg gtcactttca
    29041 catcttcggc gccggcttcc aaggctaagc ttagcagcgc atcctcttct accttcccct
    29101 tctcaacgac caagacccct tttttatgga actgccaggc caccgcgccc gcctccgcca
    29161 tattgccgcc gttcttggtc aacaaattgc ggatctcggc cacggtgcga tttcgattat
    29221 cggaggtgat ctccatcagc accgccgtcc ctcctggacc gtacccttcg agcgtgaact
    29281 cctcatacgt cacaccgggc aattcacctg tgccgcgctg gatggccttc ttcatgttat
    29341 cgccgggcat gttggcttct ttggccttcg caatggccag tcgcaagcgt ggattgccat
    29401 cggggtcccc gcctgatcga gccgcaatcg tcaactcgcg gataatacgg gtaaagactt
    29461 tcccccgctt ggcgtcgacc gctgatttat gccgcttgat tgttgcccaa tgactgtggc
    29521 cacccatggc gactcctctt ggcctggacc gaccgcctgt cgatcgagtc caacgctcat
    29581 tgatatgctg attcaaaagg tagagctaac acagtcctca cagaagtgtc aacgcgaggc
    29641 cggccccctg tggccagggg aatgtcctac tctgaataga tgagcaactg aagcccaatg
    29701 aagacaacga ggctcgccca gagcaactcc caatacgaac gttcggtgag gacggaccgc
    29761 gctcgccagc cggagaccaa cttggatgac cccgcggtga tcgccatcat gcccatgacc
    29821 acatgatgga gcccaattct actggcggca ggatgctctc cgtgcgaatg actgaacaac
    29881 atcattcctc cgacgatcgc aaacagcggg aggggcaccg tccacaccgc atgggcaaac
    29941 catccggtcc gccgcaggaa ttcaatgaca ccgacggcga aagccagcac cccataggtc
    30001 ttgtgctgga gcacctcctc atcgctgccg aaaaacgtct gcgcgaaggt catcgacccg
    30061 atgggccacg agtcatgatc gctccaaa
//
LOCUS       DAOQRM010000014        28615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_998_length_28615_cov_14.395483, whole genome shotgun sequence.
ACCESSION   DAOQRM010000014 DAOQRM010000000
VERSION     DAOQRM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28615
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_998_length_28615_cov_14.395483"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PKK81_02655"
     CDS             <1..413
                     /locus_tag="PKK81_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV31799.1"
                     /translation="QADMAPLLLEGPSDREAVERVLATIKVDVPVIRGLALSTMAGVL
                     SHAKLYVGHDSGVTHLAAALSVPTIACFGPTDSRRWAPLGRAVSIVSGAPCTCRTWSA
                     VAECRERVCLQIAPERIIEVCRLLLSERRTVPAT"
     gene            498..2252
                     /gene="argS"
                     /locus_tag="PKK81_02660"
     CDS             498..2252
                     /gene="argS"
                     /locus_tag="PKK81_02660"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNV31800.1"
                     /translation="MTQSVVQDKVSQALVGALRSAQQRGVLKLEQMPMVNLEAPKRPE
                     WGDVSCTVAMSLSASERRPPFEIAQIIIDHIEQRDALFSRAEIARPGFLNLTVKRELW
                     MDVLREIEAQGERYGHSQVGQGRRVLVEYVSANPTGPLHVGHGRGAAVGQALVRLLCA
                     TGHDVVSEYYINDAGRQMKLLGLSVLARYREACGQTVSFPEDGYHGGYIRTVAEHVKA
                     EQGAALLSLPAADAEQRSREFAYRELLALIRQDLEMFGIAFESWFSEASLLSSGAVEQ
                     VLSELRARDLLFDQEGAQWFRSSAFGDEKDRVVRKQEGDYTYLASDIAYHRDKLRRGF
                     DLLVDVWGADHHGYIPRMQAVVQAYGHPKERLRVVLVQMVNLLRGGQKVEMSKRSGEF
                     ITLREVMDEVGADAAKFFFLMRDSSTHLDFDLELAKQQSQENPVYYVQYVYARIASLR
                     RVAASRGIACPLPSQADLNVLEDPDELALIRKLSAFPVVLQASAVELEPHRMAYYLRE
                     LAGMVHPFYNKHRILPPAPDSEAAAALSTEQSGSSGRATEVMTPGLTGARLALMWAVQ
                     QVVRNGLNVLGVSAPEQM"
     gene            2266..3423
                     /gene="tgt"
                     /locus_tag="PKK81_02665"
     CDS             2266..3423
                     /gene="tgt"
                     /locus_tag="PKK81_02665"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNV31801.1"
                     /translation="MLTFRITHEESGGRARVGVLTTARSEIATPAFMPVGSLGNVRSV
                     DGEELLKMGYGLILNNAYHLYLRPGHKVVEELGGVHGFTAWPGAILTDSGGFQVFSLA
                     KFCKISDEGVMFQSHIDGSSRFISPETSIEIQEALGADIIMAFDHVVALPSSLDQVRD
                     ASKRTSLWARRCVSAKRRTDQSLFGIVQGGLDAGLRVESARDLVSVGFDGYAVGGLSV
                     GEDKADMYGMLDVTVPELPSAKPRYLMGVGMPEDLVEGVARGIDMFDCVVPSRHGRTG
                     WLFTSFGRVVIKQARYARDEQPIDPACRCPVCTRYTRAYLHHLFGVKEMLGARLNTIH
                     NLWFFAKLMEEIRSAVRGGTFQEFRREFHRTYVVDRPADDGRSDIQSTNGS"
     gene            3447..3767
                     /gene="yajC"
                     /locus_tag="PKK81_02670"
     CDS             3447..3767
                     /gene="yajC"
                     /locus_tag="PKK81_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNV31802.1"
                     /translation="MWDSVAWAQGTSGSTGAGGGLLSLVPFVLIFVIFYFMLILPQQK
                     RRKQADTMLAALKKGDKVVTASGIWGTITNLGKDTVTLQIADTTKIKIQKEHIARLRG
                     EEED"
     gene            3803..5452
                     /gene="secD"
                     /locus_tag="PKK81_02675"
     CDS             3803..5452
                     /gene="secD"
                     /locus_tag="PKK81_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNV31803.1"
                     /translation="MKKVGGRLLALVTMVVASIIFFLPSYQPLYKELPRWVREVLPDK
                     GITLGLDLQGGIHLVLEVEEDRAVEIAVERTAAGLQDLLVEKKIPAESVKRTGSSQIT
                     IGFHNAELKAQVQKLLDDFPTYVEVESAGSANKVVWELRDAEIKRIKDSAINQALETI
                     RNRIDQFGVAEPLIQRQGLKQVVVQLPGIKDAKLAKDLIKQTALLEFKLLDDENQLKL
                     DLPGRIQKGKEREDAFLKQVEGKVPEGDQILFERIVEKDSGQEWLAPYLVKKRVMLAG
                     DVLSDARVSIGQFNEPYVSVTFDAKGAREFDRITGENVKKRMAIVLDNTIYSAPVIQE
                     RISGGRAQITGTFSMEEANNLSIVLRAGALPAPLKIIQDLTVGPSLGQDSIEKGVKAT
                     LFAGLLVIVFMALYYRLSGVIANFALLLNLICLIGALAGLNATLTLPGIAGIILTIGM
                     GVDSNVLIFERIREELRQGKAVRVAIDAGYDKALLTIVDSHVTTLITGFALFLFGTGP
                     IKGFAVTLCLGIAINLFTALVGTKVVFDLFNQRRKIEQLSI"
     assembly_gap    5453..5552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5554..6420
                     /gene="secF"
                     /locus_tag="PKK81_02680"
     CDS             5554..6420
                     /gene="secF"
                     /locus_tag="PKK81_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNV31804.1"
                     /translation="MTFAISGILAFLGLIAVLQIARGAANLGIDFAGGTAVQLKFEQA
                     MKIDEARRALENNGLGSAELQEFTQDNKLLIRVKASTTIEEKVAERVMAVFSKEFPAN
                     KFVVDSSMEIGPTIGKKLQEDAMIAVLISFVGIVLYIAVRFELRFGVAAALATFHDVL
                     AVLGVFYLLDKEITLLVITALLTLAGYSLTDTVVVFDRIRENLRTRRREDEETIINQA
                     INQVLSRTIVTSLTVVIVLIPLVLAGAEVLHDFSLALLGGVMFGTYSSVFVASPLLLL
                     WPGSAGGLMKRR"
     gene            6497..9673
                     /locus_tag="PKK81_02685"
     CDS             6497..9673
                     /locus_tag="PKK81_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV31805.1"
                     /translation="MLFWSRSHSLQRKIITAIVMVGLLPLCLSLVLTYIEERRALRET
                     IGANFKEVAVEAARRIEMQVTRGVNEAQQLAATPFLRTSVTESNRTYLDKDEKTVQSM
                     IKAWQQRWRQRDKQSEFPLFINRIVTNYLIRWHDIRKADYVGIFVTDNRGALVVSSIP
                     QVEYYHGKAAWWQAVMKRGTGKVFVSDIFFDPAFGTHVVNVSAPIIDDEQHVTIGAVT
                     VLLRRDTLFHSVSEATVGHTGHAMLFNSDGVPLICPVLAPEEHAVKPELVSAISASPA
                     GWTTLANDSHGGQNSIVGFAPVRLGTELTPDSLGGKRWVTFVRQDPAESYAPLSRLVV
                     QVTVYGLGVFGVLLLTGLTVARRIARPIGLLHEGVQRIGSGRLDQQLDLKTGDEIEQL
                     ADAFNKMAANLRISFEQIEQRMEDVRRLEARYRDLIEHSPEMIYQLNKAGQFVHVNKT
                     GLDKLGYSLEEMLQMRLWDLVPKGREAEVLAYLERLVSQGRSTIETVFVAGDGHPIDV
                     EIHATALFESGGGLVHSRAFVRDVTERRLLEAQIHQYTTRLEQAVNERTRQLVASQER
                     YKALFDLVADSVFMVGEDGVIVAVNKREEQALGYSEQQVVGSSILNVVEPVHHDEMRV
                     LLEKIRAGERQVPTQEITVRDVTGRETPVEMDLILVRGSDHTWVMVQLRDITDRKRLE
                     RQMHTYQQELEAKVSERTREIEETKQYLENLLENANDVIYTLDSEQCFTYVNSKVLAW
                     GYAKEDLLGRPYLGLLSKRHRGRRLKSTLDIGVKQVYEVEVLTKAGEPRSVMVSVSPL
                     HGVEGEILGVLGIARDMTDTKKLEQQIRNSEKLASVGKLAAGVAHEINNPLGGILNCL
                     YNMRKGTLSPSRQEEYLASMEDGLRRVQRIVRQLLDFSQQHNPELALADINQVVNRVL
                     LLTDHLFVPHRVQLETALSPDIPELMIDRHMMEQVLMNLVLNAIQAMRTGGVLTIRTS
                     MDEAHCLVRIQDSGCGISSSVLPRIFDPFFTTKNEGEGTGLGLSVSLGIVERHGGRIF
                     VESEVGKGTTFTVSIPLATERHAIGRFS"
     gene            9670..11121
                     /locus_tag="PKK81_02690"
     CDS             9670..11121
                     /locus_tag="PKK81_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV31806.1"
                     /translation="MKGLKVLIVDDEPLMRLSMLDALEGIGCEVMAAATGTEGLTILG
                     TRQFDIVITDLRLPGADGLTILKACKERSPTTEVILITAHGSVDTAVGAIKLGAYDYI
                     TKPFQMDELLLIVERVGKILGLRRENLELKEVLEDRFSFGGILGCNHQMRALLEKIKL
                     VAATDSPVSIVGERGTGKELVAHAIHLNSPRRDQPLIKVCCADLPEALLEAELFGHEK
                     GAFPGALRQRRGRFELAHKGTLLIDDIDAVSPNVQARVWRVLQNRRFERVGGREHIEV
                     DVRIVCASKDDLKGMAYQGRFHKELSDQLTAVQIVVPPLRERREDVLVIAEHMLEARS
                     AALGKSLDGFAQPSRDLLLHYSFPGNVGELEQMVDRAAALGRDGESLQPWDLCGFQTC
                     PYLGGSPQAGCGFCSEGLTAMGEKPESTAPATLATAREAFERDYIAAVLKQVDGSRTN
                     AATILGVSRKALWDKCKRYGLSSAKGEAEEKDE"
     gene            complement(11123..12232)
                     /locus_tag="PKK81_02695"
     CDS             complement(11123..12232)
                     /locus_tag="PKK81_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNV31807.1"
                     /translation="MSNQTTAPLTAPSTGQPSSQSGTAQLPPPVDHLEYWKAGLRELK
                     TFRGHSHGVWSVAYAPDGQTIVSGGVDRYVRVWDIETGRLLRSLRGHTADIRALVFTP
                     DGRTLASGSEDRTIRLWNPKTGEPTKLLFTRYDHNVCSLSLSPDGLMLARGSHNKDIK
                     IWEVTTGTDLMTLLGKDQYDHHWSVCVAFSPDGVHLASGSDIGKIRIWEVLPSGEEKI
                     LHNGHWEETAEDSTETRGFFIEDDGGFQKPMEYWIGAMVFTPDAKMLITGSRDNTIRF
                     FEMPTMNELRVVRGHNGWVRSLVVSPDGKVLISAGDDNTIRFWDIATGRNFRTDKTHG
                     GAVRGIALSPDGLRLASASWDRTVKLWEGGVEPAE"
     gene            12431..13462
                     /locus_tag="PKK81_02700"
     CDS             12431..13462
                     /locus_tag="PKK81_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31808.1"
                     /translation="MATGVEVSKPSMEIRFQPALLQEVIDSFIEKTEREGDPTYYKEF
                     HELADPIYEKFTLDDREAEFKKLYQYLFGIWGFSDIIRDAFNEFPELKQRVGIVLVKG
                     VLKEDQEGVDVLRKWGSVEHDLAKQFEEKGLKGVGIKLIPRRFYDPALTRYCRHELLH
                     IHDMLDPAFGYDPDTRVGQNPGEETLILHRYRILWNISVDSRLIASGKEPMLQKEDRF
                     KEFRSWYRKIPPAQLKSVFEGLWQTSYFTHSELVEMATDTLRILDRAVDVEGGEIPET
                     TNKVMLMPGFPCPLCRFPTYTWVEDMETKLEKYILDFIRENHPGWDVEYGACDRCVEV
                     YKLRADGVM"
     gene            13475..14080
                     /locus_tag="PKK81_02705"
     CDS             13475..14080
                     /locus_tag="PKK81_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV31809.1"
                     /translation="MAADPKYGRRDFLKDSVVSVAKAAREFAAHKDAPREQPAAPVRT
                     DWLRPPGAVNEAMFLERCTRCSDCIKVCPPGAIVSDVANGTPVIFSNQVACELCNDFP
                     CIAACATEALLPVAGCFDVRMGVATVSHRVCTAGQGCHACVSKCPVEALSMDFHALHL
                     VVAPERCVGCGMCEQICKTVNDRIAIKVTPARNLSAGALGY"
     gene            14291..15268
                     /locus_tag="PKK81_02710"
     CDS             14291..15268
                     /locus_tag="PKK81_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNV31810.1"
                     /translation="MTSKQVVQPTSPSSTAIAPPQAVAIKDSPAVERALNRSKIYLLF
                     SWSLLYPEDEEFLDYLQCGEFVEDGRAALDGLRLALDGIGGDRASQKIALMKKQFDQI
                     EKLVSAECVNWQIGDLQTEHRRVFTNVITLDCPPYETLFGNDHVFAQSHVMGDIAGFY
                     KAFGVELSKDVHERLDHLSVELEFMHFLTYKESYSRCHDGIDKTEIVVDAQKKFIKNH
                     IGRWVPLFCRMLAKKSDTGLFKLIADCMSEWMDFEVAFLGVTVQPYSEADYRPATFNA
                     PEGQTYECGAQDKGNELSMLLSEVGAESFMDQQTKEKGGEKSEGPVGTA"
     gene            15494..16321
                     /locus_tag="PKK81_02715"
     CDS             15494..16321
                     /locus_tag="PKK81_02715"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNV31811.1"
                     /translation="MKAIKGVIPMDGANPIWEGVPGVIIPLSGQTITTPMHPNISVKS
                     VFVKAVSNGKDLGLRLDWSDQTKNDTAIGPQDFRDQAAVMFPVNTAGAPPFQCMGQSG
                     GTVNIWRWNAEWQKDLGKDSAGMWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGP
                     FNSGIWSGNIMSDPTLRVSSVEDLNANGFSTLTTQAHQDVIGNGVWEPSGSLKGGGYT
                     GPTWRVVVKRSLETSDANDTQFKAGASVPIAFAIWDGSNIERNGMKALSTWFTLKLP"
     gene            16598..18379
                     /locus_tag="PKK81_02720"
     CDS             16598..18379
                     /locus_tag="PKK81_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV31812.1"
                     /translation="MKVSLLFPPTWHPSQPYLSLPSLTGFLTQAGVKNVSQRDLGIEL
                     LDKVLTQSFAHGLYQQLVDKQQGLERERIGERGPGSAEQLARVIESLDRFPYLFERIE
                     LAKETLRGEGFYDIEAYRNSLFLIDKWLEVLSSLYFPTRMTVVDNQFGDYSIYSSKDL
                     IKAIRDEGQNPYISLFREHVLPSLLTDRPDLVGVSITATSQIIPGLTLCRLIKEHAPE
                     LHVTVGGSIFTRLVDNLRRCPWLFDLVDDFVVFEGETALLELVNQMDGKQDFSKVPNL
                     IYRQNGKITVNQPFYSENINQLTAPNYDGFPLDLYLSPEPVLPVQFSRGCYYKDCAFC
                     ALTLDHQNFRQRDPGKTVDDLVWLKERYGAQSFFFTDECFALSPTKRLCQQMIDRQVN
                     VKWTCELRFEKNLTRELLGQMRDAGCLKIVFGLESFNQRVMDFMKKGIKQESVRRIVD
                     DCVDLGIAVHCYVIVGFPTEKEEEALDTMNFVVGNKKLHESYGFSCQPCLFDLEKEAP
                     IMSDPGSYGIRRIMRPATEDLSLGFFYEVQEGMTPAQAEQLYQQVYEKISEVVCELPF
                     NYSMADGLLYIARAKSQALQVPQPTGS"
     gene            18469..19590
                     /locus_tag="PKK81_02725"
     CDS             18469..19590
                     /locus_tag="PKK81_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31813.1"
                     /translation="MSAVATAVERSTGRVEDQGLLFEYTIKLVLFVGFFELVLYRLVS
                     RLGMHISKIAAQHEWVGTIFQLLTSVGFALLNVVAIFVFLALVVLLVNRARAKGITGF
                     NVVTIPSVALLLILTVSFLIVPPAMLGSIAYNVVTLIALTALMLEYLSQQQEWSKRVM
                     GAVYYFGISGWLYYQIFSTAYGWMGVIAAPPFVYEANRLGEALMVLASILVFWAYGRG
                     VSFRTRNRQQRRRAIWFSAVSAVVFFGLLFMDYFLGLYNPVLAHSIRKAGEGISWIFQ
                     MGMGYTFYLPFAFYVAGLLCWSYTVIRLVTMGRLAGYGLALMFIAGYALLFSSLTLMV
                     VLGVMLMTLDRPKGAVTEQAAVTRPPLVGTQESLVGGQI"
     gene            19624..20436
                     /locus_tag="PKK81_02730"
     CDS             19624..20436
                     /locus_tag="PKK81_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV31814.1"
                     /translation="MDEQTQASGAEQGSAAVDPGDQPLSPDEEIAEIEKLLGAEPDDF
                     QARCRLGELYFSKGRLDDALTEVKKSIEMAEGLRTEMNRSLAMYYSNLGTIYATKGMI
                     DEAEAEFKRALDVHAYDVLALFNLGRVQADKRKYMESKNYYERLVEITPDDPMAWYNL
                     AGVYVELDNPQVSDYNTMDMAIQCYLRVLELDPKHLEASFKLMEIALNHRKTDLAIKV
                     MESAVEHSPDEPLAYYNLISVYDKCKMFEQAEQARQRLKERFAKKAKEGAAS"
     gene            20461..20754
                     /gene="tatA"
                     /locus_tag="PKK81_02735"
     CDS             20461..20754
                     /gene="tatA"
                     /locus_tag="PKK81_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNV31815.1"
                     /translation="MFGSLGFTELILILFIVLIIFGAGKLPQLGEGIGKAIKGFKKSV
                     HEADAIEAEAQAQAQAQQAEPVQAQAIQAPPPVAMSTPVVEQPVAAAPPAARA"
     gene            20801..22054
                     /locus_tag="PKK81_02740"
     CDS             20801..22054
                     /locus_tag="PKK81_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31816.1"
                     /translation="METDVQLAVGYIETFAGNGKARSTGDGKRAVKAGIPLPHHVALD
                     RDERWLYFAESGSDRVRRVNLAEGTVHNFAGIGETCYSGDEGPCGEAGLYLPLDVACD
                     SRNDLYVCDSGSNRIRKIDRETGIITTVVGTGEHGFNGDGPALEVNLTWPAAIAFDAD
                     DVMYIADTQAHRIRRYDSRTGLVETIAGSWTAEDEAREQPLVARNLVVLSGDAIGIDF
                     SDDNGWLMPVCSDGLSLSMYLDDGHPAMEARLYDIVGIAVDNAGDVYVVDKGSNRVRK
                     IDHKTGLISTLAGVCRYGYDGDGKPAAKSMLHAPEAIVFDQHNHAYISDTGNHRVRKV
                     DADTGFISTVAGNGDSGYDDKNMGGCGAARFVAKDAAGALKHGDGLLAVEAVVNSPVG
                     LTLDTQGYLYICERGENKIRRAKLW"
     gene            22139..22972
                     /locus_tag="PKK81_02745"
     CDS             22139..22972
                     /locus_tag="PKK81_02745"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNV31817.1"
                     /translation="MTSVHTSRTRLLTLLSSLFVLALILGGLGIPIVSSEGMIIRAQA
                     LSGDIPTAPEDPLWQKISPMTLPLSGQVITRPVWPEPTAHALAVRAVHNGTDIAFLLE
                     WQDNTKNDRLTPGTFRDGVALGLPLGDAPAFFCMGQLDHYINIWHWKADWQSDIDRRA
                     SRTTEKDKAASGEPRRFEVIPRRASSVEDLIGGGFSTLTTKEKQGRVQGKATWKDGTW
                     RVVMRRPLSSEEQENEAKLIPGRIQAVSFAVWNGENKERNGQKAIAPWFQLALDPATK
                     T"
     gene            23056..23529
                     /locus_tag="PKK81_02750"
     CDS             23056..23529
                     /locus_tag="PKK81_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNV31818.1"
                     /translation="MSKKNRTIFTRALSLGRALCVCGALFTVMGWGFQADAGTGMNGE
                     MTAEEAVQLGEEFGIAVGEVDEEVQKELKLQRPEGVAVFEVIGNSRADYAGIKVRSVI
                     KEIDKKEVRNLIDFGKAVKAAMKECNFTVGTYEPADPGDPVGWGVNFHFVGCKRD"
     gene            23554..24861
                     /locus_tag="PKK81_02755"
     CDS             23554..24861
                     /locus_tag="PKK81_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNV31819.1"
                     /translation="MNDKGRSIQKLAMGGVLLFMALLNITFHERAIAQKTDGQVPADW
                     VAEIEKIFIRSEDCKQCHDRHYEEWKGMREQTPDLKSFGRVDAALLHGTSFESPVFRT
                     VLGVWMQTNPTMQERQRCLSCHAPAVTVFPQHVEKISAQILSGKPQVEGIGCASCHLI
                     NAVETTPLPPPTYKVQPGEMLYGPYADPEENLVHPATQLPLFRGANFCTSCHFDKVKD
                     VTQKDLPGEILQGTICQDCHMEPSTGSSTSKRGAMTRAIGRHWFRGVVIPGTLLKNRN
                     LQAEWMPRIEIDATKSGAGVEGTVLVKVGSLPHSFPDGDPVLKQFFLAITIKDAQGNV
                     LGEDTKRFGLPYDKILRGPIPDPFIKGGNTRRVPFSQTLKDGAAPATIEVVLSYALIP
                     EPEAELKAKYLATLANDQERATAKKVIEEYIQPRMLTYRAKSL"
     gene            24930..26168
                     /locus_tag="PKK81_02760"
     CDS             24930..26168
                     /locus_tag="PKK81_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNV31820.1"
                     /translation="MKNRRGLLQGVVIGLVLLAVAITSYGVQQGLAQDAKAQATIEKA
                     FPSSSKCKRCHERVFEEWETSPLSRSIHTPTFRAALDAYLTSSAGKDKALCFRCHAPH
                     VREFADQAQLFVTQAQSGEPSLDGVACVQCHLIKQVDRTKQPPEPKYDLGSKTMYGPY
                     KDFAQNLAHQSMELGLFHKSDLCLNCHQVVPAAADLGKSNDLLGNWDQSKAVKSGKEC
                     QTCHMPEQVGESANGEAKRKVANHTFPGRIGQLRQEAAKLEVSTKVEGEKTTVTVAVQ
                     SLVPHNLPTTHPGWASVVLELDIKGKNLKTVFNDKRVYGRTYADAKGQKTVFDFEAIK
                     VLEETVLKPEEKRVETFTFTTPKDTKTFDVEAILSYAPVTGPSAFLQRIEAESSKGAQ
                     DPVFQSIPIAKFSENIPVAR"
     gene            complement(26258..26512)
                     /locus_tag="PKK81_02765"
     CDS             complement(26258..26512)
                     /locus_tag="PKK81_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31821.1"
                     /translation="MGLAPEYILIDQQKFSKTKLALDNHVYKNCEIDDCDVYYSGGQY
                     ELIDTHITNSRLILNHPAKGIYSAIQIFKMKSPGSSIVSE"
     gene            26877..>28615
                     /locus_tag="PKK81_02770"
     CDS             26877..>28615
                     /locus_tag="PKK81_02770"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type H(+)-translocating pyrophosphatase"
                     /protein_id="HNV31822.1"
                     /translation="MSDSAIVTFALIAAIAGIAYGLYLAMWVFKLNAGNAKMQEIAKA
                     IQEGAGAYMNRQYKTVGMVAAVLFVVLWGAGAVSDKFGMLTAIGFLIGAGASALAGYV
                     GMIIAVRANVRTAQAAHNGMNAALTVAFRGGAVTGLLLIGLGLLAITSFYTLASQMAG
                     QEKAIHALLSLGFGGSLISVFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTTVAAMVLAFTMFKGVSAPILYPLALGGVTIFAT
                     IIGILFVKVSPGGEIMPALYKGLFVAGGIAAVAFYPITSKIMEGVGGVSGISYYLAAL
                     MGLAVTLALVFITDYYTSKDYAPVKDIAKASETGHATNIIAGLAVGMQSTSAPVIVIG
                     AAILGSYWICGGAAAGGLYGVAVAAVSMLSMAGIVVAIDAFGPITDNAGGIAEMAHLG
                     KEVRDITDPLDAVGNTTKAVTKGYAIGSAALAAVVLFAEFAREVAKGSQASTFDLSNP
                     LVLVGLFLGGMLPFIFGALCMKAVGQAAGLVVEEVRRQFRTIKGIMEGTGKPEYGTCV
                     DIVTQAAIQKMMIP"
BASE COUNT         5805 a         7355 c         9084 g         6271 t
ORIGIN      
        1 tccaggccga catggctccc ctattgctgg aaggcccgtc tgatcgtgag gctgtggagc
       61 gcgtcctcgc gactataaag gtcgacgtgc cggtcatccg tgggctggct ttgtccacaa
      121 tggcaggggt gctgtcgcat gcgaaactct atgtgggaca cgattctggc gtgacgcacc
      181 tcgccgctgc cttgtcggtt cctacgattg cttgttttgg tcctactgat tcacgtcgtt
      241 gggctccgct tggtcgcgcc gtgtcgattg tatctggagc gccctgtacc tgccggactt
      301 ggagtgccgt ggcagagtgc cgtgagcgcg tgtgtcttca gatcgctccg gagcgcatca
      361 ttgaggtctg ccgactgtta ttgtcagagc gacggacggt tcctgccacc taactcgcga
      421 tcttgtctct catgtcatgg tgtgttagat tcccagtttg attttcgctg tttccacgca
      481 gacttagagg ggtttttgtg actcagagtg tggtgcaaga caaggttagt caggcgttgg
      541 tcggggcgct tcgatcggcg cagcaacgag gggtgctcaa gctggagcag atgccgatgg
      601 tcaacctcga agccccgaag cggcccgagt ggggtgatgt gtcgtgcacc gtcgccatgt
      661 ctttatctgc atcggagcgc cgccctccgt ttgaaatcgc ccaaattatt atcgatcaca
      721 tcgagcagcg ggatgcattg ttcagtcgtg cggaaatcgc acgaccgggc tttttgaatc
      781 tgacggtcaa gcgagagctc tggatggatg ttctgcgcga gattgaagcg cagggcgagc
      841 ggtatggcca cagccaagtg gggcagggac ggcgagtgct cgtggagtat gtcagcgcca
      901 atccgaccgg gccgttgcat gtcgggcatg gccgcggtgc tgctgtgggg caagcgctag
      961 tccgtctctt atgcgctacc ggacatgatg tcgtcagtga gtactacatt aacgatgctg
     1021 gccggcagat gaagttgcta ggcctgtcgg ttctggcgcg gtaccgggag gcttgtgggc
     1081 agacggtgtc ctttcctgaa gacgggtatc atggcgggta cattcgcaca gtcgccgagc
     1141 atgtgaaggc ggaacagggc gcggcgctgc tctcccttcc cgctgctgat gcggaacaac
     1201 ggagtaggga gttcgcgtat cgtgaattac tggcactcat tcgtcaggat ctggagatgt
     1261 tcgggatcgc ctttgagtcc tggttcagtg aagcctcgtt gttgtcgtcc ggcgccgtgg
     1321 agcaggtact gagcgagctg agagccaggg acctcttgtt cgatcaggag ggcgcgcaat
     1381 ggtttcgatc gtcggcgttc ggtgatgaaa aggatcgggt ggtccgcaag caggaggggg
     1441 actataccta tctggcgtcc gacatcgcct accatcgaga taagttgcga cggggctttg
     1501 atctcctggt cgatgtgtgg ggcgccgacc atcatgggta tattccgagg atgcaggcgg
     1561 tcgtgcaggc ctatggccac ccgaaggagc gactgagggt cgtgctggtg cagatggtga
     1621 atctccttcg tggaggccag aaggtcgaga tgtccaagcg gtccggagag ttcatcacgc
     1681 tgcgtgaggt gatggacgag gtcggggcgg atgccgccaa atttttcttt ctcatgcgcg
     1741 attccagtac gcatctggat ttcgatttgg aattagccaa gcagcaatcg caagagaacc
     1801 cggtctacta cgtgcaatat gtgtatgcgc ggattgccag tctccggcgt gtggcggctt
     1861 cgcggggcat tgcttgtccc cttccgagtc aggccgatct caatgtattg gaagatcctg
     1921 atgaactcgc gctcattcgg aaactgtcgg cgttcccggt ggtgttgcag gcgagtgccg
     1981 tggagttaga gccgcatcga atggcctact atcttcgtga gttggccggc atggtgcacc
     2041 ccttttataa caagcaccgg attttacctc cggcaccaga cagcgaggca gcagccgcct
     2101 tatccactga gcagtccggc tcctcaggcc gggcaactga ggtgatgacg ccgggcctga
     2161 cgggtgcccg gctggcactg atgtgggccg tgcagcaggt ggtccgaaac gggttgaacg
     2221 tgttgggcgt gtcagcccca gagcagatgt aggactgccg atcccatgtt aaccttccgc
     2281 atcacccatg aagaatccgg cggacgcgcg cgcgtcggcg tgttgaccac cgcccgcagt
     2341 gagatcgcga caccggcgtt tatgccggtc ggctcgctgg gcaatgtgcg cagtgtcgat
     2401 ggagaggaat tgctcaagat ggggtatgga ctcatcttga ataacgccta tcacctgtac
     2461 cttcgtcccg gacataaagt ggtggaggag ttgggcgggg tgcatgggtt tacggcatgg
     2521 cccggcgcta ttctcaccga tagcggtggg ttccaggtct ttagtttggc aaagttttgc
     2581 aagatttccg atgaaggcgt gatgtttcaa tcccatatcg atgggtcgtc gcggtttatc
     2641 agtccggaaa cctccattga aattcaggaa gccttaggcg cggacatcat tatggcgttc
     2701 gatcatgtcg tggcattgcc gtcctccctg gatcaggttc gtgatgcctc caagcggacg
     2761 tcgctgtggg cgcgacggtg cgtcagtgcc aaacggcgga cggatcaatc gttgttcggg
     2821 atcgttcagg gcggcctgga tgccggcttg cgggtggagt cggcgcgtga tctggtctcg
     2881 gtggggtttg acggatatgc cgtcggtggg ttgtcggtgg gcgaagacaa agccgacatg
     2941 tatggcatgt tggatgtcac ggtgcccgag ttgccgtcgg ccaagcctcg ttatttgatg
     3001 ggcgtgggca tgcccgagga tctggttgaa ggggtggcgc gcgggatcga tatgttcgat
     3061 tgcgtcgtgc cgtcgcgtca cggccgcaca ggctggttgt ttacctcgtt cgggcgtgtg
     3121 gtgatcaagc aggctcggta tgcgcgtgat gagcagccta tcgatccggc ttgccggtgt
     3181 ccggtgtgca cgcggtacac cagggcgtac ttgcaccact tgttcggcgt aaaagaaatg
     3241 ttgggcgccc ggctgaatac gattcacaac ttatggtttt tcgcgaagtt gatggaggag
     3301 atccggagcg ccgtgcgagg cggaaccttt caggagtttc gccgtgagtt tcatcggacg
     3361 tatgttgtag atcggccggc agatgatggt cgatcggaca ttcagagtac gaacggttct
     3421 taacggcggc acaggcgggg gacattatgt gggattcggt ggcatgggcg cagggaacat
     3481 cagggagcac aggcgcagga ggtggtctcc tctcgctggt tccgttcgtc ctcattttcg
     3541 tcattttcta tttcatgctg attctgccgc agcaaaaacg caggaagcag gcggacacta
     3601 tgctggctgc tcttaagaag ggcgacaagg tggtcacggc ctccggaatc tgggggacca
     3661 ttaccaattt ggggaaagac acggtgacgc tccagatcgc ggacacgact aaaatcaaaa
     3721 ttcagaaaga gcatatcgct cggctgcgcg gtgaagaaga agactgacgc acgcgacgga
     3781 gacgggaagg attggcgcac gtatgaaaaa agtcggcgga cggttgttag ccctagtgac
     3841 gatggtggtc gcctcgatca tctttttctt gccctcatac caacctctgt acaaggaact
     3901 gccgcggtgg gtgcgtgagg tcctgccgga caagggcatc accctggggt tggatttgca
     3961 gggcggcatt cacctggtgt tggaggtgga ggaagatcgg gccgtcgaga ttgccgtcga
     4021 gcggacggcg gcggggctcc aggatctgtt agtggaaaag aaaatcccgg cggagtcggt
     4081 gaaacgtacc ggttcgtcgc agatcaccat cggatttcac aatgccgaat tgaaggcgca
     4141 ggtccagaag ctgttggacg atttcccgac ctatgtggag gtggagtcgg ccgggtctgc
     4201 aaacaaggtc gtctgggagt tgcgggatgc ggagatcaag cgcatcaagg actccgccat
     4261 caatcaggcg ttggagacaa tccggaatcg catcgaccag ttcggtgtgg cagagccgtt
     4321 gattcagcga caggggctca agcaggtggt cgtccagttg cccggtatca aagacgccaa
     4381 gcttgccaag gacctcatca aacagaccgc gctgctcgag ttcaaattgt tggacgatga
     4441 gaatcagctc aagctggatt tgcccggccg aattcagaag ggcaaggagc gcgaggacgc
     4501 cttcctgaag caggtcgagg ggaaagtgcc ggaaggcgac cagattctgt tcgagcggat
     4561 cgtcgaaaag gacagcgggc aggaatggtt ggcgccctat ctggtgaaaa agcgggtgat
     4621 gctggccggc gacgtgttga gcgatgcgcg ggtctccatc ggccagttca atgaacccta
     4681 cgtgtccgtg acgttcgatg cgaagggcgc gcgggagttc gaccgtatca cgggcgagaa
     4741 cgtgaagaag cgcatggcga tcgtgctcga caataccatc tattcggcac cggtcattca
     4801 ggaacgcatc agcggcgggc gggctcagat caccgggacg ttttcgatgg aagaagcgaa
     4861 caacctgtcg atcgtgttgc gcgccggggc gttgcccgca ccgcttaaga tcatccagga
     4921 cctgaccgtc ggtccgtctc tcgggcagga ttccatcgaa aaaggtgtga aggctaccct
     4981 ctttgcgggc ctccttgtga tcgtgttcat ggccctgtat taccgcctat ccggcgtgat
     5041 cgcaaacttt gcgctcttgc tgaacctgat ctgtctgatc ggggctctcg cggggctgaa
     5101 cgccacgctg accctgccgg gcattgccgg tatcattctg accatcggta tgggtgtgga
     5161 ctcgaacgtg ttgattttcg agcgcatccg cgaagaactt cggcagggga aggctgttcg
     5221 ggtggcgatc gatgcgggat acgacaaggc gttgctgacg attgtggact cgcacgtgac
     5281 gacgttgatc accgggttcg ccctgtttct cttcggtacc gggccgatca aaggctttgc
     5341 cgtgacgctc tgcctcggta tcgcgatcaa cctctttacg gcgctggtcg ggaccaaagt
     5401 ggtgttcgac ctgttcaatc agcgcaggaa aattgagcag ctcagtatct agnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntatcacgt ttgcgatctc cggcatcctt
     5581 gcgtttctgg gtctcatcgc ggtgcttcaa atcgcgcgcg gcgcggcgaa tctgggaatc
     5641 gacttcgccg gcgggacggc ggtgcagttg aagttcgagc aagcgatgaa gatcgacgag
     5701 gcgagacgtg ctctggaaaa taacggtctg gggtcggcgg agttgcagga gttcactcaa
     5761 gacaacaagt tgctcatccg tgtgaaggcg tcgacgacga tcgaggagaa ggtcgccgaa
     5821 cgggtcatgg cagtgttcag caaagaattc cccgccaaca agtttgtggt ggattccagc
     5881 atggaaatcg gtccgacgat cgggaagaag ctccaggaag atgcgatgat cgcggtcctc
     5941 atttcgttcg tcggaatcgt actctatatt gcggtccggt ttgagttgcg attcggcgtg
     6001 gcggccgcgt tggcgacatt ccatgatgtg ctggccgtgc tgggtgtctt ttatctgctg
     6061 gataaagaga tcacgctgct ggtgatcacg gcgttgctga ccttggcagg gtattcgttg
     6121 acggataccg tcgtcgtctt cgatcgtatc cgtgagaatt tgcggacgcg acggcgtgag
     6181 gatgaagaga cgatcatcaa ccaggccatc aaccaggttc tgagccgcac catcgtcacc
     6241 agtttgaccg tggtcatcgt gctcattccg ctggtgctcg caggcgccga agtgttgcac
     6301 gatttctccc tggcattact cgggggcgtg atgttcggaa cctattcatc ggtcttcgtg
     6361 gcgagcccgt tgttgctgct gtggcccggc agtgccgggg ggctcatgaa gcgtcgctaa
     6421 tctcgggtcg tccgggcctt ggccggaacc ctatataatt atgtgagagg gccgttagtc
     6481 ggggtacgtc gcacccatgt tgttctggag ccgctctcac agtctccagc gaaagatcat
     6541 cacggcgatc gtgatggtcg gcctcctgcc gttgtgcctc tcactcgtcc tcacctacat
     6601 cgaagaacgc cgggcgcttc gggaaacgat cggggccaac tttaaagagg tggcggtcga
     6661 ggctgcccgg cgaattgaga tgcaggtgac gcgcggcgtc aacgaggcgc agcaactcgc
     6721 cgccacaccc ttcctgcgca cctcggtgac cgaatccaat cgcacctacc tggacaagga
     6781 cgaaaaaacc gtccagtcca tgatcaaagc ctggcaacag cggtggcgtc aacgggacaa
     6841 gcagagcgag tttccgctgt tcatcaaccg gattgtgacc aactatttga ttcgatggca
     6901 cgatatccgc aaggccgact acgtgggaat ttttgtgacg gacaaccgcg gcgccctggt
     6961 ggtgagttcg atcccgcagg tcgagtatta ccatggcaag gcggcgtggt ggcaggcggt
     7021 catgaagcgc ggaacgggca aggtgtttgt cagcgatatc ttcttcgacc ctgccttcgg
     7081 cacccacgtg gtcaatgtgt cggccccgat catcgacgat gaacaacatg tgacgatcgg
     7141 tgcggtcacg gtcctgttac ggcgggatac cctgtttcat tccgtgtcgg aagccacagt
     7201 ggggcacact gggcatgcca tgttgttcaa ttcggacggt gttccattga tctgtccggt
     7261 tctggcgccg gaagaacatg cggtgaagcc tgagctggtg agcgccatca gcgcgagtcc
     7321 tgcgggatgg accacgctgg ccaatgattc gcacggggga cagaattcga tcgtggggtt
     7381 tgcgccggtt cgactgggga ctgaattgac gccggatagc ctgggtggca agcgctgggt
     7441 gacatttgta cggcaggacc cggccgagtc ctatgcgccg ctctcgcggc tcgtcgtgca
     7501 agtgacggta tatgggctgg gggtgttcgg tgtcctgtta ctcacgggcc tgaccgtcgc
     7561 gcgacgcatc gcccgtccga tcggactgtt gcatgaagga gtgcagcgga tcggaagcgg
     7621 acggttggat cagcagctgg atctcaagac cggcgatgaa atcgaacaac tggccgacgc
     7681 cttcaataag atggcggcga acctgcgcat ctcgttcgaa cagatcgagc agcggatgga
     7741 agatgtccgc cggctggagg cgcggtatcg cgatctcatc gaacattccc cggagatgat
     7801 ctatcagttg aacaaggccg gccagtttgt gcatgtcaat aagaccggtc tggacaagct
     7861 cggctattcc ctggaggaga tgcttcagat gcgcctttgg gatctcgttc ccaagggccg
     7921 tgaggcggag gtgttggcct atctggagcg gttggtctcg caggggcgca gcacgataga
     7981 gaccgtgttt gtggccgggg acggccaccc gatcgacgtg gaaattcatg cgacggctct
     8041 attcgaaagc ggtggcggtc tggttcactc gcgcgcgttt gtccgcgatg tcacggagcg
     8101 gcggctgttg gaggcgcaaa tccatcagta cacgaccaga ctggaacagg cggtcaacga
     8161 gcgcacccgg caactggtgg cctcgcaaga acggtataag gcattgttcg acctggtggc
     8221 ggattcggtg tttatggtgg gggaggacgg cgtgattgtg gccgtcaaca aacgggaaga
     8281 gcaggcgctg ggctactcgg agcaacaggt cgtcggaagc agtattctca acgtggtcga
     8341 accggttcac catgacgaga tgcgggtgtt gttggaaaag atccgcgcgg gcgagcgcca
     8401 ggtgccgacc caggaaatca ccgtgcgcga tgtcaccggc agggaaacgc cggtggagat
     8461 ggacctgatt ctggtgcgcg gcagcgacca tacctgggtc atggtccagt tgcgcgacat
     8521 caccgaccgg aaacgtcttg agcggcagat gcatacctac cagcaggagc tggaagcgaa
     8581 ggtcagcgag cgaacccgcg agatcgaaga aaccaagcag tatcttgaga acctcctgga
     8641 gaacgccaat gacgtcattt acacgctcga tagcgagcag tgtttcacgt atgtgaacag
     8701 caaggtgctg gcttggggtt atgccaagga agatctgttg gggcggccgt atctggggtt
     8761 actgtcgaaa cggcatcgtg ggcgccggtt gaaatcgacg ctggatatcg gtgtaaaaca
     8821 ggtctatgag gtcgaagtgt tgacgaaggc cggcgagccg cgttccgtga tggtcagcgt
     8881 ctccccgctt catggcgtgg agggggagat cctaggcgtg ctgggaattg cccgcgatat
     8941 gacggacacg aagaagctgg agcaacaaat cagaaactcc gagaagctgg cgtcggtagg
     9001 aaaactggct gcgggtgttg cgcacgagat caacaaccca ctcgggggca ttcttaattg
     9061 tctgtacaat atgcggaagg gaaccctgtc gccgtccagg caggaggagt acctggcgtc
     9121 gatggaggat ggactgcggc gggtgcaacg aatcgtccgg caactgctcg atttctcgca
     9181 gcaacacaat ccggaactcg cactggctga tatcaatcaa gttgtcaatc gggtattgct
     9241 gttgacggac cacctgtttg tcccccaccg tgtgcagctg gagacggcgc tgtcgcccga
     9301 catcccggag ttgatgatcg atcggcatat gatggagcag gtgttgatga atcttgtgtt
     9361 gaacgccatt caagcgatgc ggaccggtgg agtcctgacc atccgtacgt cgatggacga
     9421 ggcccactgc ctggttcgga ttcaggattc aggctgcggc atttcctcct cggtgttgcc
     9481 gcggattttc gatcccttct tcaccacgaa gaatgaagga gagggcaccg ggcttggcct
     9541 gtccgtcagc ctgggtattg tggagcggca tggaggaagg atttttgtcg agagcgaagt
     9601 cggcaaaggg accaccttta ccgtctctat tccgcttgcg acggaacggc acgccatcgg
     9661 gaggttttcg tgaagggcct caaagtactg attgttgatg atgagccgtt gatgcggttg
     9721 tccatgctgg atgctttgga gggcatcggc tgtgaggtca tggcggccgc cactggaaca
     9781 gaagggctga cgatcctggg cacgcggcag ttcgatatcg tgattaccga cctgcgcctt
     9841 cccggcgccg acggactgac gattttgaag gcctgcaagg agcggagccc gacgacggaa
     9901 gtgatcctga ttacggccca tgggtcggtg gatacggcgg tcggtgcgat caagctgggt
     9961 gcatacgact acatcaccaa gccgtttcaa atggacgagc tgctgctgat tgtcgagcgt
    10021 gtcgggaaaa ttcttggatt gcgacgggaa aatcttgaat tgaaagaggt gctggaagac
    10081 cggttcagtt tcggcgggat cctcgggtgc aatcatcaga tgcgggccct gctggagaag
    10141 atcaagttag tggccgcaac cgattcgccg gtgtcgattg tcggggagcg cggaacaggc
    10201 aaggagttgg tggcgcatgc cattcatttg aatagtcccc ggcgcgatca gcccctgatc
    10261 aaggtctgtt gcgcggacct tcctgaggcg ctcctggagg cagagttatt cgggcatgaa
    10321 aagggcgcgt ttcccggggc gctgcgccaa cgacgtggac ggttcgaatt ggcgcacaag
    10381 ggaaccctgc ttattgatga tatcgatgcg gtctctccaa atgtgcaggc aagggtgtgg
    10441 cgcgtgcttc agaatcggcg attcgagcgt gtcggcgggc gcgaacacat cgaggtggac
    10501 gtccggatcg tgtgtgcgtc caaggatgat ctgaagggga tggcttacca gggacggttc
    10561 cacaaagaac tctccgatca gctcacggcc gtgcagatcg ttgtgccgcc attgcgcgag
    10621 cggcgggaag atgtgctggt gatcgccgag catatgctgg aggcgaggtc ggcggctctg
    10681 ggcaaatctc tcgatgggtt tgcgcagccg agtcgtgatc tgctcctgca ctattcgttt
    10741 cccggcaatg tcggtgaact ggagcagatg gtggaccgag ccgccgcctt ggggcgggac
    10801 ggggaatcgt tacagccgtg ggacctgtgt ggcttccaga cctgtcccta tctcggcggg
    10861 tcacctcaag caggttgcgg tttttgttcg gaaggactga cggcgatggg tgagaagccg
    10921 gagtccacgg ctcctgccac gctcgcgacg gcacgggagg cattcgagcg ggactatatt
    10981 gccgcagttt tgaaacaggt agacggcagc cggacgaatg ccgccacgat cctgggagta
    11041 tcccgaaaag ccttgtggga caaatgtaag cggtacggtc tctcctccgc caagggtgag
    11101 gcggaggaga aggatgagta ggttactcgg cgggctccac gccaccttcc cagagcttca
    11161 ccgtgcgatc ccaggaggcg ctggccaggc gtagtccgtc cggcgagagg gcgataccgc
    11221 gtaccgctcc gccatgggtc ttgtcggtcc ggaaatttcg gcctgtggcg atgtcccaga
    11281 agcggatcgt gttgtcatcc cctgcactga tgagcacttt tccgtctggc gagaccacta
    11341 acgaccgaac ccatccatta tgcccgcgca cgacgcgcag ttcgttcatc gtcggcatct
    11401 cgaaaaagcg gattgtgttg tcacggctgc cggtgatcag cattttggca tccggcgtaa
    11461 agaccatcgc gccgatccag tactccatcg gcttctgaaa tcctccgtcg tcttcgatga
    11521 aaaagccgcg ggtctcggtc gagtcttcag cagtttcttc ccaatggccg ttatggagga
    11581 ttttttcctc tccgctcggc agcacttccc agattctgat tttgccgatg tcggacccgc
    11641 tcgcgaggtg cacgccgtcc ggcgagaacg ccacacagac cgaccagtgg tgatcgtatt
    11701 ggtcttttcc cagcagggtc atgaggtcgg tgccggtcgt gacttcccag atcttgatgt
    11761 ctttattgtg actgccgcgg gccagcatga ggccgtcagg agacaacgac aggctgcaga
    11821 cattgtgatc gtagcgggta aataagagtt tggtcggctc gccggttttc gggttccaca
    11881 ggcggatggt tcgatcttcg ctgcctgagg ccagcgtgcg gccgtccggc gtgaacacca
    11941 gggcgcgaat gtcggcggtg tggcccctca gcgaacgcag gagtcgcccg gtttcaatgt
    12001 cccagacgcg gacgtatcga tcgacgccgc cactgacaat cgtctggcca tcgggagcgt
    12061 aggcgacgga ccagacgccg tgggagtgtc cccgaaacgt cttgagttct cgaagccctg
    12121 ctttccaata ttctaaatgg tcgacgggag gggggagctg tgcggttccg ctctggctgc
    12181 ttggttgtcc ggttgaaggc gcggtcaggg gggcggtcgt ttgattgctc ataaaagtct
    12241 gtgcctcgtc gctgtgctga gcgggggcca tcgagccccg gccgggaccc tcaacggttt
    12301 gcaaaaatcg ctaaggggtt tgtataatcg gcggtcgttt cgatgatcgt aagagaacga
    12361 ctgggagcaa gtcaagcaca gtacccgtgc ggctttcatc ccttcaccga accagaacat
    12421 gtgaggtctt atggcaaccg gagtagaagt ttccaagccg tcgatggaga tccgttttca
    12481 gccggcctta ctgcaggaag tcatcgactc ctttattgaa aagacggagc gggaagggga
    12541 tccgacctac tataaagagt ttcatgaact ggccgacccg atctatgaaa agttcacgct
    12601 ggacgatcgc gaggccgagt tcaagaagct gtatcagtac ttattcggta tctgggggtt
    12661 ctccgatatc atccgcgacg cgttcaatga atttcctgag ctgaagcagc gggtcggaat
    12721 cgtcctggtc aagggggtgc tcaaggagga tcaggaaggg gtcgacgtcc tgcgcaaatg
    12781 gggctccgtc gagcacgacc tggcgaagca attcgaggag aagggactga agggagtcgg
    12841 tatcaagctg attccgcggc ggttctacga tccggcgttg acgcggtact gtcgtcacga
    12901 actcctgcac atccatgaca tgctcgaccc ggcgttcggg tacgatcccg atacgcgggt
    12961 cggacagaat cccggcgaag aaacgctcat cctgcaccgc tatcgtattc tttggaatat
    13021 cagcgtcgat agccgcttga tcgcctccgg gaaggagccg atgctccaga aggaagaccg
    13081 attcaaggag ttccggtcgt ggtatcgaaa gattccgccg gcgcagttga aatccgtatt
    13141 tgaggggttg tggcagacga gctattttac tcattccgaa ttggtggaaa tggccactga
    13201 taccctgcgc atcctggatc gcgcggtgga tgtcgagggc ggagaaattc cggagaccac
    13261 caataaggtg atgctcatgc cggggtttcc ttgtccgctc tgccggttcc cgacgtacac
    13321 ctgggtggaa gatatggaga ccaagctgga aaaatatatc ttggacttca tccgcgagaa
    13381 ccatccaggc tgggatgtcg agtacggggc ctgtgatcgt tgtgtagaag tgtacaagtt
    13441 gcgggccgac ggtgtgatgt aagcggggtg catcgtggcc gctgatccta aatacgggcg
    13501 tcgtgatttc ctgaaagact ccgttgtgtc ggtggcgaag gcggcgcggg aatttgccgc
    13561 tcataaggac gcgccccgcg agcaaccggc cgcgccggtg cgaacagact ggctgcgtcc
    13621 gccgggtgct gtcaatgagg ccatgtttct tgaacggtgc acgcgctgta gcgattgcat
    13681 taaagtatgc ccgccgggag cgatcgtgag cgatgtggcg aacggtacgc cggtcatttt
    13741 ttcgaatcag gtcgcatgcg aattgtgcaa cgattttccc tgtattgctg cctgtgcgac
    13801 cgaggcgctc ctgccggttg ccggttgttt tgacgtgcgg atgggtgtgg caacggtgtc
    13861 gcaccgtgtg tgtacggcgg gccagggttg tcatgcctgt gtgtcaaaat gccctgttga
    13921 ggccctctcg atggattttc acgcgcttca tctggtggtg gctccagagc ggtgcgtcgg
    13981 atgtggtatg tgtgagcaga tttgcaagac ggtcaatgat cgtattgcga tcaaggtgac
    14041 accggcaaga aacctgtctg ccggtgcgct gggctactga ataaagtgtg tgtttactcg
    14101 ttccggaatg cgaaatgatc aggcagagta gtgaaacgtc ggcacttgcg gcaagggcaa
    14161 aggagagaga gttcatgctt gacatccctc cctcctttac ctatcatacg cctcctgtgc
    14221 cgcaggactc agatgttgcg tagtgtgcga aaaaaatgcg caacatgttg aggtgaggag
    14281 caagatacgt atgacatcga aacaggtcgt gcagcccacc tccccgtcct ccaccgcaat
    14341 cgctcccccg caggcggtcg ccataaaaga ttcgcccgct gtcgaacgcg cgctcaatcg
    14401 cagtaaaata tatctcttgt tttcatggag tttgttgtat cctgaggacg aggagttcct
    14461 ggattatctc cagtgtggtg aattcgttga agatggacga gccgcattgg acggattgcg
    14521 tctcgcgctc gatggcatcg gcggtgatcg ggccagccag aaaatcgccc tgatgaaaaa
    14581 acagttcgac cagatcgaga aactggtttc ggctgagtgt gtcaattggc agatcggcga
    14641 tcttcagaca gagcatcggc gggtcttcac caatgtgatc acgctggatt gtccgcccta
    14701 cgaaacactg ttcggtaacg atcacgtttt tgcgcagtcc catgtgatgg gcgacattgc
    14761 aggcttctac aaagccttcg gcgtcgagct ctcaaaggac gtgcacgagc gtctcgatca
    14821 cctgagcgtc gagctcgagt ttatgcattt tctgacctac aaagaatcct attcgcgctg
    14881 ccacgatggt atcgacaaga ccgagatcgt ggtcgatgcg caaaagaaat ttatcaagaa
    14941 tcatatcggc cgatgggtgc cgttgttctg cagaatgttg gcgaagaagt cggatacggg
    15001 cttattcaag cttattgccg actgtatgtc ggagtggatg gattttgagg tcgccttcct
    15061 cggagtgacc gtgcaaccgt actctgaggc ggactataga ccagctacct tcaatgctcc
    15121 ggaaggtcaa acctacgagt gcggggcgca ggacaagggg aatgagctca gcatgttgct
    15181 gagtgaggtc ggcgctgagt ccttcatgga ccaacagacg aaagagaagg gcggcgagaa
    15241 gagcgaaggc ccagtcggga ctgcgtagga gttctcggtt ttcgggcagt gcagtattcg
    15301 gttttcggca gacgttatac gttcccagtt ctttttctta ttaatctttc agaggagggc
    15361 atacgcaatg agactggtgc agacacgcaa taagcgtttg gtgtttggca ttctgttctc
    15421 tgcgctgatc gtcggcatta tgttgacgat cgggcaggtg cctctcgcgg tcagccaacc
    15481 ggtgaccatt cccgtgaagg cgatcaaggg ggtaatcccg atggatggtg caaaccccat
    15541 ctgggaagga gtgccaggtg tcatcatccc attgagcggc cagaccatca cgaccccgat
    15601 gcacccgaat atttcggtga agtcggtgtt tgtgaaggcg gtcagcaatg ggaaggattt
    15661 ggggttgcgc cttgattgga gcgaccagac caagaacgac acggcaatcg gtccgcagga
    15721 tttccgggac caggccgccg tcatgttccc ggtcaacacc gcgggagccc cgccattcca
    15781 gtgcatgggc cagtcaggtg ggacggtcaa catctggcgt tggaacgcgg agtggcagaa
    15841 ggatctcggc aaggatagcg ctgggatgtg ggacgtggat gaccaatacc ctggcatctt
    15901 ctgggattac tatttcgaag agccggctgg tggagtgacc tatccggatc gtattggccg
    15961 gagccttggc ccattcaatt ccggcatttg gtccggtaac atcatgtccg acccgaccct
    16021 gcgcgtcagc tccgtcgagg atctgaatgc caacgggttc agcaccttga ccacccaggc
    16081 ccatcaggat gtgatcggca atggtgtgtg ggagccgtcc gggtccctca agggcggtgg
    16141 atacaccggc ccgacctggc gtgtagtcgt gaagcgttcg ctggagacca gcgatgccaa
    16201 cgacacccag ttcaaggcgg gcgcttcggt gccaatcgcc ttcgcaatct gggatggttc
    16261 caacattgaa cgaaacggta tgaaagccct ctccacttgg ttcacgctga agttgccgtg
    16321 agcctagtgg cctgaagtgg ccaatcctac tcctgtgccg ggtaggaatc gtgtggtgat
    16381 tcgtaacagg aaggccccga gtcgttgcca gaaatggcaa agtgattcgg ggccttcgtg
    16441 cgttttgacg gagagtgcct caacttcggg aaggcctgaa atttcttggc gtgcacaggg
    16501 cggagggttg cattcggact ggagcggagt gtatataccg atacagtgca actcttctga
    16561 aaatactgat ttttctgctt aatataaaga taatcctatg aaagtttcac tcctgtttcc
    16621 tcctacgtgg cacccatccc aaccgtacct cagccttcca tcactcaccg gattcctcac
    16681 ccaagccgga gtcaaaaacg tctctcaacg tgacctcggc attgagttgt tggacaaggt
    16741 tttgacgcaa tcctttgcgc atgggctcta ccagcagttg gtggacaaac agcaaggtct
    16801 ggagcgagag cgaatcggtg agagggggcc cgggagcgca gagcaacttg ctcgcgtgat
    16861 cgagtcgctt gaccggtttc cctatttgtt tgagcgaatc gaactggcaa aagagacgct
    16921 gcgaggtgaa gggttctacg atattgaggc ctatcgcaat agtctctttc tgatcgataa
    16981 atggcttgag gttctctcct cgctatactt tccgacgcga atgacagtgg tcgataacca
    17041 attcggcgac tattcgatct actcctcaaa agatctgatc aaggccattc gtgacgaagg
    17101 gcagaatccc tatatcagtc tcttccgcga gcacgtgttg ccttcgctcc tcactgatcg
    17161 tccggatctg gtcggtgtct cgattacagc gacctcgcaa attattccag gcctcacgct
    17221 ctgtcgcctg atcaaggagc atgctccgga gctacacgtg acggtcggag gaagtatctt
    17281 cacccggttg gttgataatc tgcgccgctg tccgtggctc ttcgatctcg tcgatgattt
    17341 cgtcgtgttt gaaggtgaaa cagccttgct ggagttggtg aatcagatgg atggcaagca
    17401 ggatttcagt aaggtcccga atctgattta tcgtcagaac ggtaagatta cggtcaatca
    17461 gcccttttac tctgagaata tcaaccagct cacagcgccg aactacgatg gctttccgct
    17521 ggatctgtat ttgtcccccg agcctgtcct gccggtacaa ttttcacgag gctgctacta
    17581 caaagattgc gcgttctgcg ccctcacgct ggatcaccag aatttccggc agcgtgatcc
    17641 ggggaagacg gtggacgatt tggtctggtt gaaagagcgc tatggagcgc agtccttttt
    17701 cttcaccgac gaatgtttcg ccctctctcc caccaagcgt ttgtgccagc agatgatcga
    17761 tcggcaagtg aatgtgaagt ggacctgcga gttgcgtttc gagaagaacc tcacgcgtga
    17821 attgcttggc cagatgaggg acgccggctg tttgaaaatt gtgttcggcc tggagtcgtt
    17881 caatcagcga gtcatggact ttatgaaaaa agggatcaag caggagtccg tgcgccggat
    17941 cgtcgatgac tgcgtcgatc ttgggattgc cgtgcactgc tacgtgatcg tcgggttccc
    18001 cacggaaaag gaagaggaag ccctcgatac gatgaacttc gtggtgggga ataagaagct
    18061 ccacgagtcc tacgggttct cctgtcagcc ctgcttgttc gacctggaaa aagaggcgcc
    18121 gatcatgagt gatccgggca gttacggcat ccgtcggatc atgcggccgg cgaccgaaga
    18181 cctgagcctg ggcttctttt acgaagtgca ggagggcatg acccctgctc aggccgagca
    18241 actctatcaa caggtttacg aaaaaatcag cgaagtcgtg tgcgaattgc cgtttaacta
    18301 ttctatggcc gacgggctgc tctatatcgc tcgggccaag tcgcaagcgt tgcaggtacc
    18361 acagccgacc ggctcttagc gttcgtgcgg tcatcagggc aggctcctgc cgcgttgacc
    18421 ggttgtggtc agaggattat catcaacgtt ttgcacaggg ggctggagat gtctgctgta
    18481 gccaccgccg ttgaacggtc aaccgggcgg gtcgaagatc agggactgtt gttcgaatac
    18541 accatcaagc tggtgttgtt cgtcggattt ttcgaactcg tcctctatcg cctggtatcc
    18601 cgactcggca tgcatatcag caagattgcg gcccagcatg agtgggtcgg aacgatcttc
    18661 cagttgctga cgtctgtcgg cttcgccttg ctgaatgtcg tcgcgatttt tgtatttctg
    18721 gccctggtgg ttctgctggt gaacagggct cgcgccaagg gcatcacggg gtttaatgtc
    18781 gtcactattc ccagcgtggc cctcctgttg atcctcacgg tgtcctttct gattgtcccg
    18841 ccggccatgc tgggctccat cgcctacaat gtcgtgaccc tcattgcact caccgcgctg
    18901 atgctggagt atctctccca gcagcaggag tggtcgaagc gagtgatggg cgcggtctat
    18961 tacttcggca tttccggatg gctctattat caaatcttct ccaccgccta tgggtggatg
    19021 ggggtgattg ccgcaccgcc atttgtgtat gaagccaatc gtctcggcga agcgctgatg
    19081 gtgcttgcca gcatcctggt gttctgggcc tatgggcgcg gggtgtcgtt ccgtaccagg
    19141 aatcgacagc agcgccggcg cgcgatctgg ttcagcgccg tctcggcggt ggtgttcttt
    19201 ggcttactgt ttatggatta tttcctgggg ctctacaacc cggtgttggc ccattccatc
    19261 cggaaagccg gggaggggat cagctggatc tttcagatgg gcatggggta taccttttac
    19321 ctgccctttg ccttttatgt cgcggggctg ctctgttggt cgtacaccgt aatcaggctc
    19381 gtgacgatgg ggcgtcttgc gggatacggc ctggcgttga tgtttattgc gggctatgca
    19441 ttgctctttt ccagtttgac cctgatggtg gtgctcgggg tgatgctgat gacgcttgat
    19501 cgtcccaaag gtgcggtgac ggaacaggcg gcggtgacca ggccacccct cgtcggcacg
    19561 caggaatctc tcgttggtgg acaaatttaa tcttgtgagt cacccagatt aggatacgtt
    19621 gttatggatg aacagacaca ggcaagcgga gcagagcagg ggagtgcggc ggtggatccc
    19681 ggcgatcaac cgttgagccc cgacgaagaa attgcggaga ttgaaaagtt gctcggtgcc
    19741 gagcccgatg attttcaggc acggtgcagg ctgggggaac tgtatttcag caaaggccgg
    19801 ctcgacgacg cactcaccga agtgaaaaaa tcgatcgaga tggcggaggg gctccggacc
    19861 gagatgaacc ggtcgctcgc gatgtactac tccaacctcg ggaccatcta tgccaccaag
    19921 ggtatgatcg acgaggctga agcggaattc aagcgcgcgc tcgatgtgca tgcctacgat
    19981 gtgcttgccc tgttcaatct gggccgcgtg caggcggaca aacgaaagta catggagtcg
    20041 aagaattact acgagcggct cgtcgagatc accccggatg atccgatggc atggtacaat
    20101 ctcgccggtg tgtacgtgga actcgacaat ccacaggtct ccgattacaa cacgatggac
    20161 atggcaatcc agtgttatct tcgtgttctg gagttggatc cgaagcatct ggaggcgagc
    20221 ttcaaactga tggaaatcgc cctcaatcat cggaagacag atttggcgat caaggtcatg
    20281 gagagcgcgg tggagcacag cccggacgag cccctcgcct actacaactt gatcagcgtc
    20341 tacgacaagt gcaagatgtt cgagcaggcc gaacaggctc gccagcgctt gaaagaacga
    20401 ttcgcgaaga aagccaaaga gggagccgca tcctgaacat gtgagcaaag gagacacacc
    20461 atgtttggca gtcttggttt tacagagttg atcctgatcc tgtttattgt gctgattatt
    20521 ttcggggcag ggaagttgcc gcagcttggc gaggggatcg gcaaagcgat caagggcttc
    20581 aagaaatccg ttcatgaggc ggatgcgatc gaggctgaag cccaggcgca agcgcaggct
    20641 caacaggccg aaccggttca ggctcaggct attcaggccc cgccgccggt ggcaatgtcc
    20701 acgcctgtcg ttgagcagcc ggtcgccgca gcgcccccgg ccgcgcgcgc gtagcatcct
    20761 tcgacacaat gggcccagca cagaacggat tgacacagtt atggaaacgg acgttcaatt
    20821 agcggtcgga tacattgaaa cctttgccgg aaacgggaag gcgcgaagca cgggggacgg
    20881 caagcgcgct gtgaaagcag gcattccgct gccgcaccac gtcgcgctgg atcgggacga
    20941 gcgctggctg tatttcgccg aatcaggcag cgaccgggtt cgtcgcgtca atcttgcgga
    21001 gggtaccgtc cataattttg ccgggatcgg tgagacctgc tacagcggcg acgaagggcc
    21061 ttgcggagaa gccggactgt atctgccgct ggacgtcgcc tgtgactctc gtaacgatct
    21121 ctatgtctgt gattccggca gtaaccggat tcgaaaaatc gatcgtgaaa ccggaattat
    21181 caccacggtc gtgggcaccg gtgaacatgg atttaatggt gatggcccgg cgttggaagt
    21241 gaatttgacc tggcccgcgg cgatcgcctt cgacgcggac gatgtgatgt atatcgccga
    21301 cacgcaggcg caccggattc gtcggtacga ctcgcgaacc ggcctggtgg aaaccatcgc
    21361 gggaagctgg accgcggaag acgaagcccg ggagcagcca ttggtggcgc gcaacctggt
    21421 ggtgttgtcc ggcgatgcga tcggcatcga cttcagtgac gacaacgggt ggctgatgcc
    21481 ggtctgttcg gatgggctca gtttgtcgat gtacctcgat gacggacatc cggccatgga
    21541 ggcgcggctg tacgatatcg tcggtatcgc cgtcgacaat gccggtgatg tgtatgttgt
    21601 cgacaaaggc agtaatcgag tgcgcaagat cgatcataag accgggctga tttccaccct
    21661 cgccggtgtg tgccggtatg gatatgatgg agacgggaag ccggcggcga agtcgatgtt
    21721 gcacgcgccg gaagccattg tcttcgatca acacaatcat gcctatatct ctgacaccgg
    21781 caaccatcgg gtccggaagg tggatgcgga cacgggcttc atcagcacgg tggccggtaa
    21841 cggtgacagc gggtacgatg ataagaacat gggtggctgt ggcgcggcgc ggtttgtcgc
    21901 caaagatgcc gccggcgcgc ttaagcatgg tgatggtctg ctcgccgtcg aggccgtggt
    21961 taattctccg gttgggctga cgctcgacac gcaggggtac ttgtatatct gtgagcgggg
    22021 tgaaaataag attcgtcgcg ccaagctgtg gtaatttggg gaacggattc agcgtcccgc
    22081 gcacatagcg ggtgcgggag tttttacggt tagcggattg cgtcgggaca tgaggcgaat
    22141 gacatcggtt cacacatccc gcactcggtt actgacactt ctctccagcc tgttcgttct
    22201 cgccctcatt ctgggcggtc tcggtatccc catcgtcagt tcagaaggca tgatcatccg
    22261 tgcacaggcc ctctcgggag acatcccgac cgcgcccgaa gatcccctct ggcagaaaat
    22321 ttcgcccatg accttgccat tgagcggcca ggtgatcacc cggccggtgt ggccggagcc
    22381 gacggcccat gcgttggcag tccgcgccgt gcacaacgga accgacatcg ccttcttatt
    22441 ggaatggcag gacaatacga aaaacgatcg cttgacgcct ggcaccttcc gtgacggtgt
    22501 tgcgctggga ttgccgctcg gcgatgcgcc ggcatttttc tgtatgggtc agctcgatca
    22561 ttacatcaat atttggcatt ggaaagccga ttggcagagc gacatcgacc ggcgtgcttc
    22621 caggaccacg gaaaaagaca aggcggccag tggtgaaccc cggcgctttg aggtcattcc
    22681 tcggcgggcg tcctcggtgg aagatttgat cggcggcggg ttcagcaccc tgaccaccaa
    22741 ggagaagcag gggcgcgtgc agggaaaagc gacctggaaa gacggcacgt ggcgagtcgt
    22801 gatgcgtcgg ccgctgtcga gtgaagagca ggagaatgaa gcgaagctga ttccgggtcg
    22861 tattcaggcc gtgtcttttg ccgtgtggaa cggcgagaac aaagagcgta acggacagaa
    22921 agccattgcc ccctggtttc aactcgcgct ggatccggcc acaaagacgt aattcttcct
    22981 ggtaggatat gactgtcggc aggacgtgcc ggttccggtt caacctgtgc ggatgaggcg
    23041 aacccgttgg agggaatgtc caagaagaat cgaacgattt tcaccagagc cctttctctc
    23101 ggaagggctc tttgtgtgtg tggggccttg ttcaccgtga tgggatgggg cttccaggcc
    23161 gatgcgggta ccggtatgaa cggcgagatg acggcggagg aagccgtgca actcggggaa
    23221 gagttcggga tcgcggtcgg tgaagtggat gaagaggtac agaaagaact caagttacag
    23281 cggcctgaag gcgtggcggt ctttgaagtg atcggcaatt ccagagcgga ctacgcgggg
    23341 atcaaggtgc ggtcggtgat caaggagatc gacaagaagg aagtccgcaa cctgatcgat
    23401 ttcgggaagg ccgtcaaggc ggcgatgaaa gagtgcaatt ttaccgtcgg gacctatgag
    23461 ccggcagatc cgggcgatcc ggtcggctgg ggcgtgaatt ttcattttgt cgggtgcaag
    23521 cgggactaaa tccgaaccgg tcatgagcgc agcgtgaacg acaagggacg atcgattcaa
    23581 aagttggcga tgggtggggt gctgctgttc atggcactgc tcaacatcac ctttcatgaa
    23641 cgggcgatcg cgcagaagac cgatggccag gtaccggctg attgggtggc ggagatcgaa
    23701 aagattttca tccggtcgga agattgcaag cagtgccacg atcgtcatta cgaagaatgg
    23761 aagggcatgc gcgagcaaac tccggacctg aaatcgttcg ggcgggtcga tgccgccttg
    23821 ttgcatggta cctcgttcga atcgccggtg ttccggacgg tgctgggggt atggatgcag
    23881 accaatccga cgatgcagga gcgacaacgt tgtttgtcgt gtcatgcccc ggccgtgacg
    23941 gtcttcccgc agcatgtcga gaagatcagc gcgcaaatcc tgtcaggcaa gccgcaggtc
    24001 gagggaatcg gctgtgcgtc ctgtcatctc atcaacgccg tcgaaacgac gccgctgccc
    24061 cctccaacct acaaggtgca gccgggcgag atgctctacg gtccctatgc cgatccggaa
    24121 gagaacttgg tacatccggc gacacagttg ccgctgtttc gtggcgcgaa tttctgcacg
    24181 tcctgccatt tcgacaaggt gaaggatgtc acgcagaagg atttacccgg cgagattctg
    24241 caagggacca tctgccagga ttgccatatg gagccctcca cgggaagctc cacgtcgaaa
    24301 cgcggcgcca tgacccgcgc gatcggccgc cattggtttc gaggcgttgt cattccaggg
    24361 acgttgctga agaaccgcaa cctgcaggcg gaatggatgc cgcgtatcga gatagacgcc
    24421 acgaaatccg gagcgggagt ggaaggcact gtgctggtga aagtcggcag tctgccgcat
    24481 agtttccccg acggcgatcc ggtgttgaag caattcttcc ttgcgatcac gatcaaggat
    24541 gcgcaaggga atgtgttggg tgaagacacg aaacgattcg gtcttccgta cgacaagatt
    24601 ctgcgcggcc cgattccgga cccgttcatc aagggcggca atacgcgccg tgttccgttc
    24661 tctcaaacgc tgaaggatgg cgccgcgccg gcgacgattg aggtcgtgct cagttatgca
    24721 ttgatcccgg aaccggaagc ggagctcaag gcgaagtatc tggcgactct ggcgaacgat
    24781 caggagcggg cgaccgctaa aaaggtcatt gaggaataca ttcagcctcg tatgctgacc
    24841 tatcgggcca aatcactcta agctcccctg cagcgggccg tgcaccacag gcagcgagga
    24901 ttgagtactg tgcgaaggaa acgccgacgg tgaagaatcg acgaggattg ctgcagggag
    24961 tcgtgatcgg cctggtgttg ctggcggtgg ccatcaccag ctacggcgtg cagcagggat
    25021 tagcccaaga tgccaaggcc caggccacga tcgaaaaggc ttttcccagc tcaagcaagt
    25081 gtaagcgatg tcatgagcgt gtgtttgaag agtgggagac gtcgccgctt tcgcgttcga
    25141 tacatacgcc gaccttccgg gcggcgctcg atgcctacct gacctcctct gccgggaaag
    25201 acaaagccct gtgctttcgc tgtcatgccc cgcatgtgcg cgagtttgcc gatcaggctc
    25261 aattgttcgt gactcaggcc caatccggtg agccatcatt ggatggtgtg gcctgcgtgc
    25321 aatgtcatct catcaagcag gtcgatcgga cgaaacaacc gcctgagccg aagtacgatc
    25381 tcggcagcaa gaccatgtat ggcccataca aagatttcgc gcagaacctt gcgcatcaat
    25441 ccatggaatt ggggctcttc cacaagtctg acctctgctt gaattgccac caggtggtgc
    25501 ccgctgcggc ggacctcggc aagagtaacg acttgttggg gaattgggac cagagcaaag
    25561 ccgtgaagtc gggcaaggaa tgccagacct gtcatatgcc tgagcaggta ggggaatccg
    25621 ccaatggaga agccaagcgg aaggtggcga atcatacgtt cccgggccgc atcggtcagc
    25681 tcaggcagga agcggccaaa ctggaagtct cgaccaaagt agaaggggag aaaactaccg
    25741 tgacggtagc ggtgcaaagc ctggtcccgc acaacctgcc gaccacccac cccggttggg
    25801 cgagtgtcgt cttggaattg gatatcaagg gcaagaacct gaagaccgtg ttcaacgaca
    25861 aacgcgtcta cggccggacg tacgccgacg ccaaaggcca gaagacggtg ttcgattttg
    25921 aggccatcaa ggtgcttgaa gaaacggtcc tcaagccgga agaaaagcgc gtggaaacgt
    25981 ttaccttcac gacaccgaag gacaccaaga ccttcgacgt cgaggccatc ctcagttacg
    26041 ctcctgtgac cgggccatct gcctttttgc agcgcattga ggcggaatcc tccaaggggg
    26101 cgcaggatcc tgtgtttcaa tccatcccaa ttgcgaagtt cagcgagaac attccggtcg
    26161 ccaggtagcg ttgcccgtta ttcgtgaagc gtatcttgtc caagaccacg tgtcttacgg
    26221 tcacacgagg gccgaacgac gcgtcacgaa gtacggatta ctcggagacg atgctggaac
    26281 cgggggattt catcttgaag atttgaatgg cgctgtagat gcccttggcc gggtggttga
    26341 ggatgagtcg tgagttggtg atgtgcgtat cgatcaattc gtactgtccg ccgctgtagt
    26401 acacatcgca gtcgtcgatc tcgcaattct tatacacgtg attgtccagc gccagcttgg
    26461 ttttactgaa cttttgctga tcgattaaga tatattccgg cgccagtccc atcgagtact
    26521 cctcaatccg cgcgcactat aacaaagtcg ctgtccttcc gcaaacgccg agccgcgagg
    26581 cttcgggcga ggtgtcgtgc ctccaagcct tcacgccggc catctttcgc cttgatccga
    26641 ttccgacctc gctatagaat ggcgccactt tcccgctcgt tcgcaccgtg tctgttgtcg
    26701 ggcatgcagt atttcttcct gctgcattct cagctcgcga taggtttagt ccacggagat
    26761 tctgtgacat tcgtcgaccg ttgtacgtga ctggttcatg ttcgcgacgg ggtattctcg
    26821 tagacacttg agctggattc tcagtttcac cattctgtta acaggaggag aacactgtga
    26881 gcgactcagc aattgtgacc tttgctctga ttgcggcgat tgccggtatt gcctatggcc
    26941 tctatcttgc catgtgggtg ttcaagctca acgcgggtaa tgcgaagatg caggaaattg
    27001 cgaaagcgat tcaggagggt gccggcgcct atatgaatcg ccagtacaag acggtgggta
    27061 tggtggcggc ggtgctgttc gtagtgctct ggggggcggg cgccgtatcg gataagttcg
    27121 gaatgctgac agccatcgga ttcttaatcg gcgctggggc ctcggcactc gctggttatg
    27181 tgggtatgat catcgcggtc cgggcgaacg tgcggacggc gcaagcagca cacaacggca
    27241 tgaatgcggc gttgaccgtc gcgttccgcg gcggcgcggt gacggggctg ttgctgatcg
    27301 ggctcggcct cttagcgatc accagtttct acacactggc ttcgcagatg gccgggcagg
    27361 aaaaggcgat tcatgccttg ctcagccttg gctttggcgg cagtttgatc tcggtgtttg
    27421 cgcgcgtcgg cggtggcatc tacaccaaag cggccgacgt gggcgcggac ctggttggca
    27481 aagtggaagc aggtattcct gaagatgatc ctcgcaatcc ggcggtcatc gccgacaacg
    27541 tgggcgacaa cgtcggcgat tgcgcaggca tggcggcgga ccttttcgaa acctacgcgg
    27601 tgacgaccgt ggccgcgatg gttctcgcgt tcacaatgtt caagggcgtc agcgcgccga
    27661 ttctgtatcc gctcgcgttg ggcggtgtga cgattttcgc gaccatcatc ggcattttgt
    27721 ttgtgaaggt gagcccgggc ggcgagatca tgccggccct gtacaagggg ttgtttgtgg
    27781 ccggcgggat tgcggccgtg gcgttctacc cgatcacctc gaagattatg gagggcgtcg
    27841 gcggggtgag cggtatcagt tattacctgg cggcgttgat ggggctggcg gtcacgctcg
    27901 cattggtgtt catcaccgat tattacacgt ccaaggacta tgcgccggtg aaggatatcg
    27961 ccaaggccag cgaaacgggt catgcgacca acatcattgc cggtctggcg gtgggcatgc
    28021 agtcaacgtc cgccccggtg atcgtcattg gcgcggcgat tctcggcagt tattggattt
    28081 gcggcggcgc ggcggcgggt gggctctatg gcgtcgctgt ggctgcggtg tcgatgctct
    28141 cgatggcggg gatcgtggtg gcgatcgatg cgttcggccc gattacggac aatgccggtg
    28201 ggatcgctga aatggcccat ctggggaaag aagtgcgcga catcacggat ccgctcgacg
    28261 cggttgggaa tacgacaaag gcggtcacca agggctatgc gatcggctct gccgcactcg
    28321 cggcggtggt cttgtttgcg gagttcgccc gtgaggtggc caagggtagc caagccagca
    28381 ccttcgatct ctccaatccc ttggtactgg tcgggctctt tctcggggga atgttgccgt
    28441 tcattttcgg cgcgttgtgt atgaaggcgg tcggccaggc ggccgggctg gttgtggaag
    28501 aggttcggcg tcagttccgg acgatcaaag gcattatgga aggtaccgga aagccggagt
    28561 acggcacctg cgtggatatc gtgacgcagg cggcgattca gaagatgatg attcc
//
LOCUS       DAOQRM010000015        26474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1136_length_26474_cov_18.473485, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000015 DAOQRM010000000
VERSION     DAOQRM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 26474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26474
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1136_length_26474_cov_18.473485"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKK81_02775"
     CDS             complement(<1..230)
                     /locus_tag="PKK81_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31823.1"
                     /translation="MTFRKVDIIFVVLALLVVGGVALLPSPRDRNPKVPANSAHQAIA
                     VESQCTTCHAPAGARPLPVQHPKRQDCLHCHAR"
     gene            complement(227..3067)
                     /locus_tag="PKK81_02780"
     CDS             complement(227..3067)
                     /locus_tag="PKK81_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNV31824.1"
                     /translation="MTLILPNTSSVVVSGLRQLLGQAKVRNDVPTLTAYAVDASIYRM
                     QPQAVVLAEHEGDIDTVVRFAVERGIPLTPRAAGTNLTGSAIGSGIILDISKMNRILE
                     VNEEERWARVQPGIVLAELNKQLGRRNLLFGPDPSSGDMCKLGGMLANNSAGPHTLIY
                     GAVKDNVQAMRVCLPSGSWLSASSMNLNDPGLDQTLAAHPSLKPILDLVQRQRSLIDS
                     KKPTVSKNSCGYNLFGLADGLSRGFFDLPKLFVGSEGTLGVVSEATLRLVPKPQGTLT
                     ALIHFRRLEEVGEAVPHLLSLRPSALEVMDANTLNLIGRSAHGIPADAAATLLAELDS
                     SEGEGDLRERADQMAAICGRYQLCGDLTIAYDKEQRDQLWKARKALYPTLYRFDPRKK
                     PINFVDDVVVPATRISELIRYLETFFEGQHVPVAIFGHIGNGNAHITPLLDVNDRQDF
                     DKMVRAYHEIHGAVLSRFDGSICGEHGDGRVRAEYVRKMFGEELYQLFVQVKQTLDPA
                     NVMNPGIKISETPFTEHIDYQRLSKSCATCAKCNSVCPVYDVFQSEDMSSRGWFEIVT
                     AKDYSYLDSKRVVEACLNCKSCRTICPAGVDVSELILQRRAENPNQGSRWLFALQAKL
                     PVFEAILKLLARTQSWWDRPAPRALLERLAAPVMKRIASTAKLPADMVLPKLATTHLR
                     NRYPELTQPSAKKAGPRVAYFHGCAANYFDDGVGDAVISVLRKHHVEPELPPQRCSGT
                     PIETYGHVDLVKDNARFNLQSLAGYDQVVTGCASCTLMLKEYKKFFESGPEQQAADVL
                     AKKVVHITEFVARSDQHPPMGTPEGQGGKVTYHSSCHLRAAGVTKEPRQLLKQLPGSD
                     YVEMTDADRCAGGAGTYIVKDYETSQKVFDRKRRNIEQSGAETVATSCPACMIQLSNG
                     MRGRVAVKHVAQLLNDAYEAGAATETRPGAGQ"
     gene            3175..4146
                     /locus_tag="PKK81_02785"
     CDS             3175..4146
                     /locus_tag="PKK81_02785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNV31825.1"
                     /translation="MSPPILYISRLLPDPVMAAARQQFQLFNEPRDIAPSWETFKAGL
                     READAAICTLTDRVDTALLAEPTRLKILANYAVGYNNIDLAAATAKGIVVTNTPDVLT
                     DSTADLTWALILAVARRVAEGDAYVRAGAWPGWAPTQMLGTDVSGKMLGIVGMGRIGQ
                     AVAKRAAGFNMRICYSSRTPHAPGQGSSQWEQRALPDLLREADFVTLHVPLSPETHHL
                     IGPRQLALMKPTAFLINTSRGPVVDEGALVDALLRRRLAGAGLDVFEQEPVFHPSLRD
                     LRQVVLLPHLGSATLATRVRMGMICLENVAAVCAGRLAPNQVNRTQA"
     gene            complement(4202..6115)
                     /locus_tag="PKK81_02790"
     CDS             complement(4202..6115)
                     /locus_tag="PKK81_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV31826.1"
                     /translation="MAVDRRTVLQNAQLFASKGQYEAAIAEWRKLTTDTPADGTIFNS
                     IGDLQLKRNATPDAVAAFLQAAGAFRSEGSVLKAIAAYKKILKVDPTKIDIYRHLGDL
                     NAERGLLSSAVQDYLTLGKHYFKDGRNKEALEIYRKIISQDPSNLDAQLRVAELCEQE
                     QLVDEAIRVYLQLGRERSAAQQYEQANAMYAAVLRLNPENAEAKEIVTMIEAGGGAPL
                     NLARVAKPSAAAAKPGEGSDLMAEATRRIEEGQYAGAEAMLTQLLSREPGNPEICQLL
                     ARLHLLQGNLQVALGEYRFLAGAALRAQDYVMAESLINDYLKVEPASVPMLELLGELY
                     QEKGDAATAAQHFGRAVEILLEHPEPGMPTLPAELFEKVQALAPGSAIARKLAPAFDP
                     TASSSTEPEVTEPMVMTPEPVAIQLEPEPQPEPAPAPVIAAPAVDMPFRLQDSSATPA
                     APAPPAAPVLAQVAPPPPPAPAPPVAQAAPVEPPPAPVPAAQPIAQVDAETQYALGMA
                     YKDMGLLEEAKEEFVSSMKDSGFFVDSCLMLGLCLKEQNRFDQAIQLLEKLIADSRCR
                     GGNAQLVRYELAMLYEKTGARDRALAMYQSIPSFHDVPRRVEALRTLGTNGVAHPERP
                     ASSQTSPLPLAGH"
     gene            complement(6292..6510)
                     /locus_tag="PKK81_02795"
     CDS             complement(6292..6510)
                     /locus_tag="PKK81_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNV31827.1"
                     /translation="MKTKGTVKWFNDRKGFGFIRLDGGDDVFVHYSALQGEGFKTLKE
                     GENVEFDIVQGAKGPQAANVLKDLATAS"
     gene            6821..7906
                     /locus_tag="PKK81_02800"
     CDS             6821..7906
                     /locus_tag="PKK81_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNV31828.1"
                     /translation="MLHTILDRYIFRELFSPFGISLAALCFVMLTKELLRLVELLVTK
                     GIGFLSVLKVFAHLLPSFLVLTLPIAGIIATITAFGRLSLDKELVAMRAAGFSLLRLS
                     QSVFLFAGLVCGLTLVMAQYGQPWSNVNLKKVALNLLRDELVLELDRGVFNEAIPKMT
                     IYVPDAQEGQDNRGIFVADERNPADPRIIVAQQYQVMTDPASSQVALRLMNGVIHSRP
                     QNPEEYQKISFTSYDLKLSLSASLYGAEERTPIDVIRAKLESTGWTDTNALRRLMEYY
                     KDLAFPAASLVFCILGVPVGIVSKRSGSIGGFAVGVLVVIAYYVLNVACEFLVTTLWI
                     SPFAGAWLPNVIFTLVTILWFYRVSRQ"
     gene            7911..9002
                     /gene="lptG"
                     /locus_tag="PKK81_02805"
     CDS             7911..9002
                     /gene="lptG"
                     /locus_tag="PKK81_02805"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNV31829.1"
                     /translation="MNILFRYMLREYVKIFGMCFAGLMTIYLVIDFFEKVRRFLRYDA
                     HALDVLAYFVLKMPAISYQIAPLAVLMATLLTIGLLSRSHEITAMRSCGISLYWITSP
                     FLFLGTVLAMVLFLFSSTVIPLALAKAEQIKTTRIEKRELPVSVKAAQPWVSLGGQTL
                     MNIDTIDPGGAVLRKIRLYRLSPTFELEQIAEAQRAVYSPQGWVLEDGIRRSFRQDGT
                     VSVNSFQTEPLPLAQIPDDFTTWLELDSETMTLPEIRAYVDRLHLGGTILPRLLTDYY
                     GRIAFPCVTFIMVVVGIALSLRRTGVRGSGMAMGIGQAMAVGFFYWSTHSVAIALGRG
                     GALAPMLAGWMANLVFLTFGFYLLLKVRY"
     gene            9157..10413
                     /gene="fabF"
                     /locus_tag="PKK81_02810"
     CDS             9157..10413
                     /gene="fabF"
                     /locus_tag="PKK81_02810"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNV31830.1"
                     /translation="MSTRVVITGLGVVSPIGIGVPQFWKAALEGRSGISAITSFDPFP
                     LEGYRSRVAGRILGFSPEQYLPAGQGDRVDRYAQFALVAAKEALADADFRMEREEPHR
                     VGVIVGAGMGGMVMGEREITQLYEQKRPHRVHPNFIPVITLNSASGIVAMAYGAKGPN
                     LTISTACSSSAHALGQAMHAIRAGTADAVIVVGADASITPLVFAGFCSLRALSTKYND
                     APERASRPFDRGRDGFVMGEGAGALIVESLAHAKKRKARMYAEVAGYAATSEAHHMVI
                     PREDGEEVAITMRLALKDAGVTPAQVDYINAHATSTTVGDAVEVKAIRLLFKSRADKL
                     AVSATKSLVGHTLGAAGSLAGIVSALTLTSGQIHPTLNLDDPDPACALAGLSAQPQTR
                     KTKVALINAFGFGSNNAAVVLKSLST"
     gene            10477..10818
                     /locus_tag="PKK81_02815"
     CDS             10477..10818
                     /locus_tag="PKK81_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNV31831.1"
                     /translation="MAKTSDVSDKIIQALADYLKRDAASIQTTHHLRDDLGLDSMAVI
                     EMLYRIEEVFNLQIPDQDLVGLTTVGQVIAYVQGRVAPPKAPAKAAAKTPAKAAAKPP
                     AAKRSTKSKKA"
     gene            10823..11905
                     /gene="lpxD"
                     /locus_tag="PKK81_02820"
     CDS             10823..11905
                     /gene="lpxD"
                     /locus_tag="PKK81_02820"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNV31832.1"
                     /translation="MTTAPRHTPLTLLELHSYVGGELVGSPDATVTGVASLEEAGPGD
                     LAFLTSERNLKSPQTATIGALLVGRRLPDCPSPQLVVDNPAYAFARAAQQFFARPTRM
                     RGIAQGITRGEDVTIGADVSIWPGVTLGDRVSIGARVTLYPGVFIGDDSAIGDDALLY
                     PNVVVREGCRLGARVIVHSGTVIGSDGFGYVQYQGRHQKIPQLGGVLIEDDVELGSNV
                     SVDRATFGNTVIKRGTKIDNLVQIAHNVVVGEHNILVAQVGIAGSTTLGKYVMVGGQA
                     GLADHLQIGDQVMIAAKSGVTRSLEPNQIVSGAPVMPHATFLKAQAVIPQLPELRQRV
                     RELEERLAKLEQIRQPPAKSRKPRRK"
     gene            complement(11924..12448)
                     /locus_tag="PKK81_02825"
     CDS             complement(11924..12448)
                     /locus_tag="PKK81_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31833.1"
                     /translation="MAKLISLKQQEGHDARATAYIKAYMLFPAGILGLISMIGGVGGL
                     GYQLIATDSYTWWTFLQSSGLLLLGGVLGWVQTTYHRWILTNRPEVFAARMRQPTVSK
                     SGKPKRESAASQEQASGSPWAPGAYMVGLAVLLAGSMVSMIYGAVHPIAACFLPWAGF
                     FWAKLFFWKSMLKN"
     gene            complement(12937..15009)
                     /locus_tag="PKK81_02830"
     CDS             complement(12937..15009)
                     /locus_tag="PKK81_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNV31834.1"
                     /translation="MEQNHYRQAEDALMRVAAAVQRQEPVNLTELTHLAGSIVESIQE
                     NDQLVVEALSSPVGPPLVTNLVNVSILATKVGIGLGYYGAELRRLALAGLLHDIGIFA
                     VPQHLLTKTGRLSSDERALVEQHPRLGYGVIERLGADYAWLAEVVLQAHERGKGQGYP
                     NRLKGREINELAQIIGLVDIFDALVSPRPYRRRLLPHEAIRELLNTERTAFPREIMKG
                     LVEQLSVYPLGTKVRLSSGEEGVVVRITPRYPSRPVLRLTGAQETAVTDPPRFLDLSQ
                     LPQVSVAETVDSPALERVTFATAPMAAEPATPTSASDQFAALLESLDAIAGVIQAAVE
                     QPKAAAQPAQVQSTIDAPDQGPHVRREILGLFVLEAREWLNQIQTALERLDTTSEQGR
                     RAKLTTILWQSVSNLAQSAATVGLTAVEDMATRLLPLLQAAAKHERTVAAHHVASLRE
                     GLLEISKTVQALQALERPPQDHLEAAVAVQQPVTEMPEPMVAEAPAPVEADVPCDAPS
                     TSLPAASILDALRQLHQARGRSLQPVRDVLETVIQRAEQEMARGTGVVDARAIGRLLQ
                     ELDELDERFLAHMQAHIPAVIATLRHVALTSEDRVFPPQALEPIFVEIEALSDAADRV
                     AAANISLFLHGLRTFLRVTAQHKPMVIRERLAAVEERLATLIPLAQQWVDVGRVERAA
                     IFDILPMA"
     gene            complement(15209..16156)
                     /locus_tag="PKK81_02835"
     CDS             complement(15209..16156)
                     /locus_tag="PKK81_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31835.1"
                     /translation="MSTEPEQTEDDFQKELIELFGQEAQEWLIQIHSALTELESQPDT
                     DRHAQLVDAVVRGITSLGGSAATVNLPDVERATFALLPFIDTLKDRTTATKQDFGTVR
                     EQFCLVIASVTMATGITLDLGPEQGAPPQPEPAIDFLTLLNALRMLQDEQVAKEVPSR
                     SLIPQVMHRLEQEARQGESQIQMTAFQQLLGELQSTDARCLDSLRQALPGIAQNLNRL
                     RVEGLTVLEPGNVLGTCMQQIEQLQGIAKQVNATPLVTFLTGLQNFLALIVQRRIVIA
                     SQRVQAVEARILAVMTTIEDWITAGQQERDLMSRLLPAA"
     gene            complement(16216..17706)
                     /locus_tag="PKK81_02840"
     CDS             complement(16216..17706)
                     /locus_tag="PKK81_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31836.1"
                     /translation="MPKILIADDSIAVRKVAERLLTEAGMGVTLAANGSEALALLSKD
                     RPDLIVSDVIMPDKSGYEVCAYIRGQANLADLPVLLISGIVNDEVSRQAESCKADGVL
                     KKPFQGSSLKDRVLDLLTKRPQKPAPVPELPQQHVAVEPSIVAEVAAAPAIHVPEELE
                     EARVDTQEHRPSLEPITSSLDDPHAIPATMTRDTMVDAAPAFHAAEPATPAHDHEHHH
                     AILAERDTRIGELEAQLDEERRRSLEQTQQIGHIQRALDEQHEQVAALSSHAHTLDQS
                     LADERAQRTALAEQLDGVSRQMHRIGELETALADEQHRAQQLSQQVSEGARHAARIVE
                     LEAHLEAEREAANQLVQQITGLEQVETRVHELETTLAAEREQAEMQRRERVELEQVAD
                     KVPALEEACAKAHAQITELDAALSAEREAAAALLVQVKQLEATAQRASELDLALVSEQ
                     ERSMQLEKRAMEAEHMAEQSTRRFEDMARKLGEIAGLASQLGNGKR"
     gene            complement(17735..18211)
                     /locus_tag="PKK81_02845"
     CDS             complement(17735..18211)
                     /locus_tag="PKK81_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV31837.1"
                     /translation="MLRTQLGRQPHKGSKGATEARMQHMVVFSVGGQRLAARTEEIGG
                     VMPWTGSTAVPSDTPFVRALVRQEKGCLPVFDLAAKFKRSLQDGEPLCLVVKHVDGPL
                     AICIDSQVPSLHMVARSAVQYRAGNDPDIAGTCIAGEEELPIINLTTLGVSSNRPA"
     gene            complement(18228..18800)
                     /locus_tag="PKK81_02850"
     CDS             complement(18228..18800)
                     /locus_tag="PKK81_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV31838.1"
                     /translation="MGLRGHKTAVVSSGLAVRFLVALMGPTHVAFPSHWVRGIVTPAE
                     GGQDGHVTWANASYERTDLARRLTIQAKGVTAETRIVLYANEQRSRSFAVDKVVGLID
                     VERTLIQPLPAQFRGGERERLLGLFVESSYIALIANPFWVLELPSRTNVLDVFALRVS
                     ERRPGEFDSRLRLPSAALEEASAMSAGSAK"
     gene            complement(18848..22237)
                     /locus_tag="PKK81_02855"
     CDS             complement(18848..22237)
                     /locus_tag="PKK81_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNV31839.1"
                     /translation="MSADFDRDQLLDIFVAEAGDDMGRFWKALHPDGKAHPEPGDVAE
                     FHTVGHKLKGAALLYGFPALGQLGALLEDTLERVQEIPAARWPEAMQLMREIAASFRG
                     QVQQIGRGGGEDASVVERFVRRHSDLMPAVSSDPSAASVQRSDQPADEYLIPVLDEEV
                     LSYFSPEAEEYLSTIQTLLQRLDSDLANADTIHQLYRVAHTLKGSAYTVGFEVVGDLS
                     HPIESCMMAVREGAVTIAPHWIAVLRQAVDVIRSLMARDAQSLTRLRQEVPRIRTKLR
                     ELEQGVSQGESAAAAAETRRPEEKSVQESVPAATSGPESAVHSMSEADSQELSEAYLI
                     PALDSEVMSYFAPEAQEYLESLEADLLRVDKDAGNPDTIHQLFRTAHTLKGSAYTVGF
                     QAIGDLTHHIEDFMGAVREGQLVLLPGHTDVLLRSIDVIRALMRRDVSMVGRTRQRFA
                     NSLQELKQLGQAQPAPDQDHPIVSSIAEGASVEEIREADGQDSAKGVDGKTGEEREVI
                     RVSRDRLERLLNLVGELVIDRGRLEQRLRTLDQLATQVLANKNRLTDAVRTFEDKHTF
                     SFQPSPASSSEGAPQAYPGVSDFGSLEFDKYDDFNILARRISEVTADITESMSQLSGS
                     IHRAQDDMGSLQQLTLGMRDEIARARMVPIGTPFTRFRRAAREMARATGKEVNLVTSG
                     EHTEIDTGVVERLVDPLVHLVRNAVYHGIEPAGIRRSQGKPAAGTIYLHAAHRGNSVL
                     IEVEDDGAGLDIVKIKAKAVKLGLVRADVADSLPESEIIKFIFLPGFSTAEAVGDQAG
                     RGVGMDVVKRVIETMNGHIEVESVYGQGTKFTMHLPLTLLIATALLVRVGKERYAIPL
                     PSVREVTMSTASTIQQMGDRSVLQIGDEAIEVYPLGSLIRRDAGTIEGATPVVVVRTS
                     TGALGCAVDELLGRQEIVIKSLGGLKPYERSVFGGATIDPEGRVVLVLDVSRLTTREY
                     HEAMSVIQNPASSPMLEGPAQPASPQLPDTQLPLLLIDDSLSIRKFVGRMLESAGYIV
                     ETATDGEEGCRKALVQQYQLIITDLEMPKLNGFEVIQALRARPQTQTTPILVMTTRAG
                     EKHRQMAVSVGASGYIAKPVEERALIQEVRKWTGRESVVKK"
     gene            complement(22290..24164)
                     /locus_tag="PKK81_02860"
     CDS             complement(22290..24164)
                     /locus_tag="PKK81_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV31840.1"
                     /translation="MIGQGVKNRFQDMKTKTKLLVSFGLVSVIIMIMASVGVFTLRQL
                     STQSQTVYADYTVPLAEFAQMGTALTKHHQILLDIASATKQGDFAQEVTKLPPLKAQI
                     DKVVNNYKSTTLRVSRSGRDEAKDLTLFEPALKKYFQDADGALSAMADSFDRNVLSST
                     QAEQMRALGILALTVNLTPSFENAVKRHNEQVTAIEAVAKDLNDDAQGLASNGTLILV
                     AGGLVAVALGLFVGYLLATFLSRSISHIANVATQAAGGNLQARAKIQSHDELGQMATA
                     FNSMLDRITALVSTEEERDLMQKRLMQFLVLVSEVGKGDLTRRGEVTADMFGNLADGF
                     NLMIARFGQLLKQVREAADRVNKSAGTLRDSAGQMSGTARVQAEESVRTLGAVEQLAA
                     GMRQVATTAGASSDSAKQVLSATERGNVAVQETVRDMQSIRSAVQRMSKQVKGLGDRS
                     LEISQIVSTIRDIANQTNLLALNAAIEAAGAGEAGARFAVVADQVRKLAESSTQATRE
                     IADLVKVIQTETQDAVVAMEHETQAVEAGSASALRTGDVFAEISDIAKRSSELAQNIA
                     SAASEQTASTEKVGRAIKEFTGGAVATQKQTDSTRLTIEDMAKLAEGLNSSVAQFKLA
                     "
     gene            complement(24260..24697)
                     /locus_tag="PKK81_02865"
     CDS             complement(24260..24697)
                     /locus_tag="PKK81_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV31841.1"
                     /translation="MCVLTLGGELFAIDLRHVSEVFEVEAVTVVPSMPSLLTGVTNLR
                     GTVVTLVDLRGSLGLPVTETALPFAVVIRHGTRQVGVLVDHVPEIHTVSREHLLPAMQ
                     AGPAGARPCVSAVLRLDERLGGVLEVPQVFAQVDGGSAILSIA"
     gene            complement(24809..25171)
                     /locus_tag="PKK81_02870"
     CDS             complement(24809..25171)
                     /locus_tag="PKK81_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV31842.1"
                     /translation="MSKIVVVDDSYAELQLIEGYLKSANHTVVSYPNTDKLEDKLALD
                     KPDLIVLDVVMPGRNGFQACRDLKSDDRFKGIPIVLCTSKGQESDKFWGQQQGANGYV
                     VKPFKAEDLVAAVKRALN"
     gene            complement(25236..25970)
                     /locus_tag="PKK81_02875"
     CDS             complement(25236..25970)
                     /locus_tag="PKK81_02875"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV31843.1"
                     /translation="MAKHIIKRIVMAALTVTCMGAAAPASANTEVETAELLIKLLQVG
                     RGVISEYQATINDASKGDKGFSGDFVSNQVIERFRKATKIDLSRPATVPQAPLFLAMV
                     ESEKEVIDEAQPVINKQGVGFKGIIPAVFARKSGERFYRKTGIRVKLTGTDYRFPGNR
                     PDEFESEVLRIFADTRHPKGQPYSKTTMLDGKPVLRMMDPEYAASTCLSCHGDPKGER
                     DITGAKKEGWHEGDLAGAISIVIPMR"
BASE COUNT         4993 a         8272 c         7865 g         5344 t
ORIGIN      
        1 cgcgcgtggc aatgcaggca atcctgccgc ttgggatgct gcaccggcaa ggggcgcgcg
       61 ccagccggcg catggcaggt cgtacactgg ctctctaccg cgatcgcttg gtgcgcgctg
      121 ttcgccggca ccttcgggtt gcggtcgcgc ggcgacggca gcagggccac gcctccgacc
      181 acgagcagcg ccagcaccac aaaaatgata tccactttgc gaaaagtcat tggccggctc
      241 ctggtcgggt ctctgttgca gcccccgcct cataggcgtc gttgagtaat tgcgccacat
      301 gtttcaccgc cacgcggccg cgcatgccgt tgctgagctg aatcatgcag gccgggcaac
      361 tggtggccac cgtctccgcc ccgctctgct cgatattccg ccgcttccgg tcgaagacct
      421 tctgcgacgt ctcgtaatct ttcacgatgt aggtgccggc gccgcccgca caacgatcgg
      481 cgtcggtcat ctccacatag tccgatcccg gcagctgttt gagcaactgc cgcggttctt
      541 tggtcacccc ggcggctcgc aggtgacagg acgaatggta ggtgaccttc ccgccctggc
      601 cttccggcgt ccccatcggt ggatgctggt cggatcgcgc cacgaattcc gtgatgtgga
      661 ccaccttctt cgccaacaca tccgccgcct gctgttccgg cccgctctcg aaaaactttt
      721 tgtattcctt gagcatcagc gtgcaggagg cgcagcccgt gacgacctga tcatagcccg
      781 ccagggattg cagattgaag cgggcgttgt ctttcaccaa atctacatgc ccgtaggttt
      841 cgatcggcgt cccggaacaa cgctgcggtg gaagctccgg ctctacgtga tgtttgcgta
      901 agacggaaat gaccgcgtcc cccaccccat cgtcgaagta gttcgccgcg cagccatgga
      961 aataggcgac gcgagggcca gcctttttcg ccgacggttg cgtgagctcc ggataccgat
     1021 tgcgcaaatg agtcgtcgcg agcttgggca ggaccatgtc cgccggaagt ttggctgtgg
     1081 aggcgatgcg cttcatcacc ggcgcggcca ggcgttcgag cagggcgcgt ggagcgggcc
     1141 gatcccacca tgactgcgtc ctggccaata gtttaagtat cgcttcaaat accggcaact
     1201 tcgcctgcag ggcaaagagc cagcgcgagc cctgattggg attctcggcg cgccgctgca
     1261 ggatcagctc cgacacatcc accccggccg gacagatcgt gcggcaggat ttgcaattca
     1321 agcaggcttc caccacccgc ttcgagtcga ggtagctgta gtccttcgcc gtgacgattt
     1381 cgaaccaacc gcgcgagctc atgtcttccg actggaacac gtcgtagacc ggacagacgg
     1441 agttacactt cgcgcaggtc gcacaggatt tcgacagccg ctggtagtcg atgtgttcgg
     1501 taaacggcgt ctcgctgatc ttgatgccgg ggttcatcac gttcgcggga tcgagcgtct
     1561 gcttcacctg cacgaagagt tgatacaact cctccccgaa catcttgcgc acatattccg
     1621 cccgcacacg gccgtcgccg tgctcgccgc aaatggaccc gtcgaaccgg gagagcaccg
     1681 cgccgtggat ctcatgatag gcccgcacca tcttgtcgaa gtcctggcgg tcgttcacgt
     1741 cgagcaaggg cgtgatgtgg gcgttgccgt tgccgatatg gccgaagatt gccaccggca
     1801 catgctgccc ctcaaagaag gtttccaggt agcggatcaa ctcgctgatc cgtgtggcgg
     1861 gcacgaccac gtcgtccacg aagttgatcg gcttcttgcg cggatcgaac cggtagagcg
     1921 tgggatacag ggcctttctg gctttccaga gttggtcgcg ctgctccttg tcgtaggcga
     1981 tggtgagatc gccgcacagt tgatagcgtc cgcagatggc agccatttga tcggcccgtt
     2041 cgcgcaggtc gccctcgcct tcgctgctgt cgagttcagc cagcagcgtg gcggccgcat
     2101 cggcgggaat gccatgcgcc gaccgcccga tcaagttcag cgtgttcgca tccatgactt
     2161 ccagcgcgct cggccggagg ctcaacaaat gcggcaccgc ttcccccact tcttcaaggc
     2221 ggcgaaagtg aatcaacgcc gtcagcgtgc cctgtggttt cggcacgaga cgaagcgtcg
     2281 cttcgctcac cacgcccagc gtgccctcgc tgccgacgaa cagcttgggg agatcgaaga
     2341 agccccgact cagcccgtcg gcgagaccaa acaggttata cccgcaactg ttcttgctca
     2401 ccgtcggctt cttgctgtcg atcaggctgc gctgccgctg gacgaggtcg aggatcggct
     2461 tgagcgacgg atgggccgcg agggtctgat ccagtcccgg atcgttgagg ttcatcgagg
     2521 aagcggagag ccaggagccg gacggcaggc agacacgcat ggcctggacg ttatccttaa
     2581 cggcgccgta gatgagggtg tggggcccgg ccgaattgtt ggcgagcatg cccccgagct
     2641 tgcacatgtc tccgctggac gggtcgggac cgaagagaag attgcgtcgc ccgagctgct
     2701 tgttcaattc agccaggacg atccccggct gcacgcgggc ccagcgttcc tcctcattca
     2761 cttcgaggat acgattcatc ttggagatat ccaggatgat gccggagccg atcgcagagc
     2821 cggtcagatt ggtgccggcc gcgcgcggcg tgagggggat gccccgctcc accgcaaatc
     2881 gcacgacggt atcgatgtcg ccttcatgct ccgcgagcac gacggcttgc ggctgcatcc
     2941 ggtaaatgct ggcatcgacg gcgtaggcgg tcagcgtggg cacgtcgttc cggacctttg
     3001 cctgtccgag gagttgccga aggccgctga cgacgacgga tgaggtgttg ggaaggatga
     3061 gggtcatgct tgtgattacg ttgcgtcatt gtagtcgctc ggcagagagc aggacaagtg
     3121 agttgttgcg tcgcacacga ttcgctaaga tacgtgccat tcgaacgagg cgccatgtca
     3181 cctcccatcc tttatatctc ccgactgttg cccgatccgg tcatggcggc agcacgccag
     3241 cagtttcaac tcttcaatga gccgcgcgac atcgcaccct cgtgggagac tttcaaggca
     3301 ggccttcgcg aagccgatgc ggccatctgc accctgaccg atcgcgtgga tacagctctg
     3361 ctggccgagc cgaccagact caagatcctg gcgaactacg ccgtgggtta caacaatatc
     3421 gacctcgccg ccgcgacggc caagggcatt gtcgtcacga atactccgga cgtcttgacc
     3481 gactccactg ccgatctcac ctgggccttg atcctggcgg tggcgcggcg ggtggcagaa
     3541 ggcgacgcct acgtgcgagc cggcgcttgg cccggttggg caccgactca gatgctgggg
     3601 acggatgtgt cgggaaaaat gctggggatc gtcggcatgg gccggatcgg acaggcggtg
     3661 gcgaagcggg ccgccgggtt caacatgcgc atttgttaca gctcacggac cccgcatgcg
     3721 ccgggacagg gctcatcgca atgggaacag cgcgccctgc ccgacctgct gagagaagcc
     3781 gacttcgtga ctctgcatgt acccctcagc ccggagactc atcacttgat cggtccgcgg
     3841 cagctggcac tcatgaagcc cacggcgttt ctgatcaata cctcgcgagg tccggtggtg
     3901 gatgaaggcg cgttggtcga tgcgctcctg cgacgtcggc tggccggcgc ggggctggat
     3961 gtgtttgaac aggagccggt ctttcacccc agcctgcgcg acctccggca ggtggtgctc
     4021 ctcccgcacc tcggctccgc aaccctggcg acacgtgtgc gcatggggat gatctgtctg
     4081 gagaatgttg ccgcagtctg cgccgggagg ctggctccga atcaggtgaa tcgcacgcag
     4141 gcgtaggacc tctaccctcg catcagccca tcagcagcag gatttctagg taaagcgagg
     4201 tttagtggcc cgctagtggc aggggactgg tctgactgga agccggacgt tccggatggg
     4261 cgaccccgtt ggtgccgagg gtgcgcagcg cttcaacccg gcggggcacg tcgtgaaacg
     4321 agggaatgga ctgatacatg gcgagcgctc gatcgcgtgc gccggttttc tcgtagagca
     4381 tggccagttc ataccgcacg agctgcgcat tgcctccccg gcagcgcgaa tcggcgatga
     4441 gcttctccag caattgaatg gcctgatcga accggttctg ctccttgagg caaagtccca
     4501 gcatcaggca ggaatcgacg aaaaacccgg agtccttcat cgaggagacg aactcttcct
     4561 tcgcttcttc caacaacccc atgtccttgt aggccatgcc cagcgcgtac tgcgtttccg
     4621 cgtccacctg ggcgatgggt tgcgcagcag gaacgggggc cggaggaggc tcaacgggcg
     4681 cggcctgggc aacgggcgga gccggagcgg gcggaggcgg cggggcgacc tgagccagca
     4741 caggcgccgc cgggggcgct ggagcggctg gagtagccga gctgtcctgc aatcggaacg
     4801 gcatgtctac cgcaggcgcg gcaatgaccg gagcgggagc cggttccggt tgtggttccg
     4861 gttcgagctg gatcgcgaca ggctccggtg tcatcaccat cggctctgtg acttccggtt
     4921 cggtggacga tgaggcagtc gggtcgaacg ccggcgcgag cttgcgggca atggcactgc
     4981 ccggggccaa ggcctggacc ttctcgaaca gctcagccgg cagcgtgggc atgcccggtt
     5041 ccggatgttc cagcaaaatt tccacggccc tgccgaaatg ctgcgctgcc gtggcggcat
     5101 cccctttttc ctgatagagc tcgcccagca gttccaacat cggcacggat gccggttcga
     5161 ccttgagata gtcgttaatc aacgactcgg ccatgacata gtcttgcgcg cgcagcgcgg
     5221 cgccggccag gaatcgatat tcacccaacg cgacctgcaa atttccctgc aacagatgca
     5281 gccgcgccag cagttggcag atttccggat tgcccggctc ccggctcagc agctgggtca
     5341 acatggcttc ggcgccggca tactgccctt cttcgatccg gcgcgtcgct tcagccatga
     5401 gatcgctgcc ttcacccggc ttcgccgcgg ccgcagacgg tttggcgacg cgtgccaggt
     5461 tcaagggcgc gcctccgccc gcctcgatca tggtgacgat ttctttggct tcggcgttct
     5521 ccggattcag gcgcaagacc gccgcataca tggcattggc ctgttcatac tgttgggcgg
     5581 cggaacgctc ccgtccgagt tggagataca cgcgaatcgc ttcgtcgact aattgttcct
     5641 gctcacagag ttccgccacg cgcagctgcg catccagatt cgacggatcc tgtgaaatga
     5701 ttttgcggta gatctccagg gcttctttgt tgcggccgtc cttaaaatag tgcttgccca
     5761 gggtcaaata atcctgaacg gcgctgctca acaaccctcg ctccgcattc aagtcgccga
     5821 ggtgtcgata gatgtcgatc ttggtgggat cgaccttgag aattttttta taggcggcaa
     5881 tggccttgag gacagaccct tcggagcgga aggcgccggc ggcctgaaga aaggcggcga
     5941 cggcgtcggg cgtggcattg cgtttcagct ggaggtcgcc gatggagttg aaaatcgtcc
     6001 cgtcggccgg ggtatcggtg gtgagttttc gccattcggc gatggcagct tcgtactgcc
     6061 ccttggaggc aaagagctgc gcgttttgga gaactgttcg gcgatcgaca gccaagaaga
     6121 ctcctcaccc gacgttgaga gtcaacgcta acagtctgcc ttttctttgt caaacaaatg
     6181 gataaaagag caacaccgcg aacgcggcgt ccagagcgga cgaaatcaac gatggcaaac
     6241 cggcggcccc tccggaacac ggaggggccg cggattacga tgccccggca atcaggaggc
     6301 cgttgcgaga tccttcaaca cgttcgccgc ttgcggcccc ttggctccct gcacgatgtc
     6361 gaactcgacg ttctcaccct ctttcaacgt cttgaatccc tcgccctgca gcgcggaata
     6421 atgcacgaag acatcgtctc cgccatcgag ccggataaac ccgaacccct tacgatcgtt
     6481 gaaccatttc actgtcccct tggtcttcat acggtcctcc ctttctcaac gcacagacat
     6541 aatgagcgca tcagctcgcc ggctgagtcg catggcatgg taatgatgac gataaaagga
     6601 gaaataaaac cgacgagcgt gaaagctctt gcgaggcgtt gagacaatat tcaacccgaa
     6661 ccatcgtgac ctactgaaaa gtgcgcagcg atgtaccatg gggtgaaaat cttgtcaagc
     6721 gggaagtttc gagaatggct ctcagatctg tttacaagct ccgtagactc ttctatagtg
     6781 cggcaactca agtctccgat gtaggatttc gaccgccctc gtgctgcaca ccatcctcga
     6841 tcgctatatt ttccgcgaac tcttctctcc gttcggcatc agcctggctg cgctctgttt
     6901 cgtcatgctc acgaaagagc tgttgcggct ggtggaactg ctggtgacga agggcatcgg
     6961 ctttctctcg gtcctgaaag tgttcgccca tttgctgccg tcgtttctgg tcctcaccct
     7021 gcccattgcg ggcatcattg ccaccattac cgccttcggc cgcttgtccc tcgacaagga
     7081 gctcgtggcg atgcgcgccg ccggcttcag cctcctgcgg ctgtctcagt cggtgtttct
     7141 tttcgccggg ctggtctgcg gcctgacgct ggtgatggcg cagtacgggc aaccctggag
     7201 caacgtcaat ctcaagaagg tggcgctgaa cctgttgcgc gacgaactcg tgttggaact
     7261 cgatcgcggc gtctttaatg aagccattcc caagatgacg atctacgtac cggacgccca
     7321 ggagggacag gacaaccggg gcattttcgt ggccgatgaa cggaacccgg ccgatccacg
     7381 tatcatcgtc gcgcagcagt atcaggtcat gaccgatccg gccagcagcc aggtggcgct
     7441 acggctgatg aacggcgtca tccatagtcg cccccagaat ccggaggagt atcagaagat
     7501 ttcgtttacg tcctacgact tgaagctgag cttgagcgcg agcctgtacg gcgcggagga
     7561 acgcacgccg atcgacgtca ttcgcgccaa gctggagagc accggctgga cggataccaa
     7621 cgcgttgcgg cgcttgatgg aatattataa ggacctggcc ttccctgcgg cctcgctggt
     7681 gttctgtatc ctgggagtgc cggtcggcat cgtgtccaaa cgttcgggca gcatcggcgg
     7741 gtttgcggtg ggcgtgctgg tggtcatcgc ctactatgtc ctcaatgtgg cctgcgagtt
     7801 cctggtcacc accctgtgga tttccccgtt cgccggcgcc tggttaccca acgtgatctt
     7861 caccctggtg accattctgt ggttctatcg ggtgagccgc caatagtcct atgaacattc
     7921 tgtttcgcta tatgctgcgc gagtacgtga agatcttcgg catgtgcttc gccgggctca
     7981 tgacgattta cctcgtgatc gacttcttcg agaaggtgcg ccgcttcctg cggtacgacg
     8041 cccatgccct cgatgtgctg gcctacttcg tcctcaagat gcccgctatt tcgtaccaga
     8101 tcgcgccgct cgcggtcctg atggcgaccc tgctcacgat cggcctgttg tcgcgcagcc
     8161 atgaaatcac cgcgatgcgc agttgcggca tcagtctcta ctggattacc tccccgttcc
     8221 tgtttctcgg taccgtgctg gcgatggtcc tgttcctctt cagttcgacc gtcattcccc
     8281 tcgccctcgc caaggccgag cagatcaaaa cgacccggat cgaaaaacga gagttgccgg
     8341 tctcggtgaa agcagcgcag ccctgggtca gcctgggcgg ccaaacattg atgaacatcg
     8401 acaccatcga ccccggcgga gccgtgttgc gaaaaatccg cctctatcgc ttgagcccca
     8461 cgttcgagct ggagcagatt gcggaggcac agcgcgcggt gtattcgccg cagggctggg
     8521 tcctggaaga cggcatccgg cgctcgttcc ggcaggatgg gacggtctcg gtgaacagtt
     8581 ttcagacgga acccctgccg ctcgcgcaaa ttcccgacga cttcacgacc tggctggagt
     8641 tggattcgga aacgatgacg ctgccggaga ttcgtgccta tgtcgatcgg ctgcatctgg
     8701 gcggcacgat cctcccgcgc ctgctgactg attattatgg ccggatcgcc ttcccctgcg
     8761 tgacctttat tatggtggtc gtcggcattg ccttgagcct ccggcgcacc ggcgtgcgcg
     8821 gcagcggcat ggccatgggc atcggacaag ccatggccgt aggattcttt tattggtcca
     8881 cccattccgt ggcaattgcg ctgggacgcg gcggggcact tgcgccgatg ctggccggct
     8941 ggatggccaa tctggtattt ctgacgtttg gcttttatct gctgttgaag gtccgttact
     9001 gagaggatcg cgaaatagtc cgccagcggc gttcgccatt cgacagtcgc agcatcgctc
     9061 gcgagtgcct gatgcttaca gcattgactg tggcgccacc gtacggtaag tagacaggcc
     9121 gcacaaggca tcaccggcaa gcagtgaaag ggttctatgt cgacgagagt cgtcatcaca
     9181 ggtctgggag tcgtctcccc catcggcatc ggggtgccgc agttctggaa agcggcattg
     9241 gaaggccggt ccgggatctc ggccattacc tccttcgatc cgttcccgtt ggaagggtac
     9301 cggtcgcgcg tggccggacg cattctcgga ttttcccccg aacagtatct cccagccggt
     9361 caaggcgacc gggtggaccg ctacgcccag ttcgcccttg tggccgccaa ggaagcgctg
     9421 gcggacgccg acttccgcat ggagcgggaa gagccgcacc gtgtcggggt catcgtcggc
     9481 gccggcatgg gcggcatggt gatgggcgaa cgggaaatca cgcagctgta tgaacagaaa
     9541 cgcccgcacc gggtacatcc caactttatt ccggtcatta cactgaattc ggcttccggc
     9601 atcgtggcca tggcctatgg ggccaaagga cccaacctga cgatttccac ggcttgctca
     9661 tcgagcgccc atgcccttgg tcaggccatg cacgccattc gcgccggcac ggcggatgcg
     9721 gtgatcgtcg tcggcgccga tgccagtatt acgcccctgg tctttgccgg attttgctcg
     9781 ctgcgcgcac tttcgaccaa gtataacgat gcaccggaac gagcctcacg acccttcgat
     9841 cgcggacggg atggctttgt catgggtgaa ggcgccggcg cgctgattgt cgaatcgctc
     9901 gcccacgcca agaaacgcaa ggcccggatg tacgcggaag tggccggata cgccgccacc
     9961 agcgaagctc atcacatggt gattccacgc gaggacggcg aagaggtggc catcacgatg
    10021 cgcctggcct tgaaggatgc gggcgtcacg cctgcgcaag tcgattacat caatgcccat
    10081 gcgacttcca cgacggtggg tgatgcggtc gaggtgaagg ccattcgtct cttgttcaaa
    10141 tcccgggcgg ataagctggc ggtgagcgcc acgaagtcac tcgtggggca tacgctgggt
    10201 gccgcgggat cgctggccgg gatcgtctcg gccctgaccc ttaccagcgg gcagatccac
    10261 ccgacgctca accttgacga tccggatccg gcctgcgccc tcgcggggct gtctgcgcaa
    10321 ccgcaaaccc gcaaaaccaa ggtcgcgctg atcaacgcgt tcggcttcgg cagtaacaat
    10381 gccgcggtcg tcttaaaatc tctgtcgacc tgacccgcgt cgggcgacga cattcgtgca
    10441 cctgcgatgt gccggcaaga aacgaaggaa ccggttatgg ctaagacttc agacgtttcc
    10501 gacaagatca ttcaggcgct ggcggactat ctcaagcggg atgcggcgtc gattcagacg
    10561 acccatcacc tgcgggacga cctgggtttg gattcgatgg cggtgatcga aatgctgtac
    10621 cgcatcgaag aagtctttaa tctccagatc cccgaccagg acctcgtcgg cctgaccacc
    10681 gtcggccagg tgatcgccta tgtgcagggc cgcgtcgccc ctccgaaggc tcccgcgaag
    10741 gcggccgcga aaactccggc gaaagccgcc gcgaagccac ctgccgccaa gcgttctacc
    10801 aagagcaaga aggcctagcc gtatgacgac cgcaccgaga cacacgccgc tgacactcct
    10861 ggaactccac agctatgtcg gaggagagct ggtcggttca ccggatgcga ccgtcaccgg
    10921 agtggcgagc ctggaagagg ccggaccggg cgacctcgcc ttcctgacct ccgaacgcaa
    10981 tttgaaatct ccgcagaccg ccactatcgg ggccctgctc gtgggacgtc gcctgccgga
    11041 ttgcccgtca ccgcaactcg tggtggacaa tcctgcgtat gcctttgcac gggccgccca
    11101 gcagtttttt gcgcgaccga cccgcatgcg cggcattgcc cagggcatca cccgcggcga
    11161 ggacgtcacc atcggcgcgg atgtgtcgat ctggcccggc gtgaccctcg gggaccgcgt
    11221 ctccatcggc gcgcgggtga ccctctatcc gggggtcttt atcggcgacg atagcgccat
    11281 cggtgatgat gccctcctct accccaacgt ggtggttcgt gaaggctgcc gtctcggagc
    11341 tcgcgtgatc gtgcacagcg gcacggtgat cggcagtgac ggattcggct acgtccagta
    11401 tcagggacgt caccagaaga ttccgcaatt gggcggcgtg ctcatcgagg atgacgtgga
    11461 actgggctcc aatgtgagcg tggatcgcgc gacgttcggc aacaccgtca tcaagcgcgg
    11521 gacgaaaatc gacaacctcg tgcagatcgc ccacaacgtg gtggtgggtg agcacaatat
    11581 tctcgtcgcg caagtcggta tcgcggggag tacgacgctg ggaaagtatg tgatggtggg
    11641 tggccaggcc ggcctcgccg accatttgca gatcggcgat caggtgatga ttgcggctaa
    11701 gtccggcgtc acaagaagtc tggagcccaa tcaaatcgtc tccggcgcgc cggtgatgcc
    11761 ccatgcgacg tttctgaagg cccaggcggt gattccgcag ttgccggaac tacgccagcg
    11821 cgtccgcgag ttggaagagc gccttgcgaa actggaacag atccgacaac ctcccgccaa
    11881 gtcccgcaag ccccgtcgca aatagcccgc gtcagccgca tcgttagttc ttcagcatcg
    11941 acttccaaaa gaacagcttg gcccagaaaa atcccgccca gggcaggaaa caggccgcga
    12001 tcggatggac cgcgccatag atcatgctga ccattgagcc cgcgagcaat accgccaggc
    12061 cgaccatata ggcgcccgga gcccagggtg acccgctggc ctgttcctga ctggccgcac
    12121 tctctcgttt gggtttgccg ctcttactga ccgtcggctg ccgcatgcgc gccgcaaaca
    12181 cctccgggcg attggtcaag atccaccggt ggtacgtcgt ctgcacccac ccgagcaccc
    12241 cgcccagcag cagcaacccg gaactttgga gaaaggtcca ccacgtgtag ctgtcggtgg
    12301 cgatgagctg atacccgagc ccgccgaccc cgccgatcat ggaaatcagg ccgaggatgc
    12361 cggccggaaa taacatgtac gctttgatgt aggccgtcgc ccgcgcgtcg tgcccttcct
    12421 gctgcttgag agagatcagt tttgccatgg gcgtgatggt cgtacaatcg caacctcaac
    12481 cgcaagcgaa aatgtgtcgg accgggacaa atttcagcga gcagcgaagc aagcatacct
    12541 tttccgtgca gggcccctct tcactcgacg agcaagaggc gtgagcacag ggggcgcagg
    12601 ccgacggagt tgattccctc caagctcgca cagcaacacg tgttcagcag gcggtggatc
    12661 gatcgttcag gattgacgtg gggaaccggc gatctcaact gagtggcatc attcttccct
    12721 gtccacaaga actgtggacg gactgtgcgc aaccatgtgg acagacctcc gccgacagtg
    12781 gctgcgtagg aatgcccgca atgtgctcaa taattaggcg gcatgacgtg agatttctca
    12841 gcaggatgga acagtgttca tctttgagcg gttgagggca ggcgacgcgg agaggcaggc
    12901 aacgcgggta tcaattggga gcgcttcgtc aagccgctac gccataggga ggatgtcaaa
    12961 aatggccgcc cgttccacgc ggccgacatc gacccattgc tgggccagcg gaatgagtgt
    13021 ggccaatcgt tcttccacgg ctgccaatcg ctcgcggatc accatcggct tgtgctgggc
    13081 cgtgacgcgg aggaatgtac gcaggccgtg caggaacagg ctgatattcg ccgcggccac
    13141 ccggtcggcc gcatcggaaa gcgcctcgat ctcgacaaaa atgggttcca acgcctgggg
    13201 tgggaagaca cgatcctcac tggtgagagc gacatgcctg agcgtcgcga tcaccgccgg
    13261 gatatgcgcc tgcatgtgcg cgagaaatcg ctcgtccaac tcatccaact cctgcaacag
    13321 tcgcccgatg gcacgcgcat ccaccacgcc tgtccctcgc gccatctcct gctcggcacg
    13381 ctgaatcacg gtttccaaca catcgcgcac cggttgcagc gatcgacctc gcgcctgatg
    13441 caactgccgc aaggcgtcaa gaatagatgc ggccggcaac gaagtggaag gcgcgtcgca
    13501 cggaacgtca gcctcgacag gggccggcgc ttcagcgacc atcggctcag gcatttcagt
    13561 cacaggctgc tgcacagcga cggcagcttc gagatgatcc tggggtggcc gctcgagcgc
    13621 ctgaagcgcc tgcaccgtct tgctgatttc gagcagcccc tcccgaagcg acgccacatg
    13681 atgagccgcc acggtgcgct catgttttgc ggcggcctga aggagcggaa gcagtcgggt
    13741 cgccatgtct tccacggccg tcaacccgac ggtcgcggcg gattgcgcga ggttgctgac
    13801 gctctgccat aaaatcgtcg tcaacttggc gcggcgcccc tgttcacttg tcgtgtcgag
    13861 ccgctcaagc gccgtctgga tctgattgag ccattcacgg gcctccaata caaacaggcc
    13921 gagaatttcc cgccggacat gcgggccctg gtcaggcgcg tcgatcgtcg actggacctg
    13981 cgcaggctgc gcagctgcct tgggttgctc caccgcggcc tgaatcacgc cggcgatcgc
    14041 atccagactt tcgagcaagg cggcaaactg atccgatgcg ctcgtcggcg tggcgggctc
    14101 ggcagccatc ggtgcggttg caaacgtgac gcgctccaat gccggtgaat cgacagtttc
    14161 tgcgaccgac acctggggca actgactgag atcgagaaac cgcggcggat cggtcaccgc
    14221 tgtctcctgt gcgccggtca gacgaagcac cggacgggat gggtagcgag gcgtgattcg
    14281 caccaccacc ccttcttcgc cgctgctcag gcgcactttg gtacccagcg ggtagaccga
    14341 gagttgctcg accaatcctt tcatgatttc acggggaaac gcagtacgct ccgtgttcaa
    14401 taactcacgg atcgcctcgt gcgggaggag gcgccggcgg taggggcgcg gactgaccaa
    14461 cgcatcgaag atatcgacga gtccgatgat ctgcgccagc tcattaattt cacgcccctt
    14521 taaacggttt gggtacccct gccccttccc gcgctcatgg gcctgcaata ccacttcggc
    14581 cagccaggca tagtccgcac cgagacgttc gatcacccca taccccaaac gcggatgttg
    14641 ctcgaccaga gcccgctcat cggagctcag acggccggtc ttcgtcagta gatgctgcgg
    14701 cacggcaaaa atgccgatgt cgtgcaggag cccggccaac gccaaacgcc gcaactccgc
    14761 gccgtaatag ccgaggccga tcccgacctt cgtcgcgaga atgctcacat tgactagatt
    14821 ggtaaccagc ggagggccga caggactgga gagggcttca acgactaact gatcattttc
    14881 ctgaatcgac tcgacgatgc tgccggcaag atgggtcaac tccgtcaggt tcaccggttc
    14941 ctgccgttgc acggcggcag cgacccgcat cagcgcatcc tcagcctgcc ggtagtgatt
    15001 ctgctccatt tcctcttagc ctccagcctc gacgcgcttc gtcacgcggc acgcctttgt
    15061 tttcacctct gcatggtcca acgctccgag tcaggcgaat cagcgaagcc gtccggaatg
    15121 atccccatgg tggaggtgcg ccttcaactc aacgaagcct gtccgggaag tcctgtccga
    15181 ggccggtcgc gcactgtgag gagcgtcctc aggcggcagg cagcagccgg ctcatcagat
    15241 cacgttcctg ttgtccggcg gtgatccagt cctcgatcgt ggtcatcacg gcgagaatgc
    15301 gtgcttcgac ggcctggaca cgttgggacg cgatcacgat acgacgctgg acaatcaagg
    15361 caaggaagtt ctgaaggccg gtgagaaagg tcaccagcgg ggtggcattg acctgcttgg
    15421 caatgccttg gagttgttcg atctgttgca tgcaggtgcc gagcacgttg cctggttcca
    15481 aaaccgtcaa tccctcgacc cggagcctgt tcaggttctg cgcaatgccg ggcaatgctt
    15541 gtcgtagtga gtccaggcat cgggcgtccg tgctttggag ctcacccagc agctgctgga
    15601 acgcggtcat ctgtatctgg ctctcccctt gtctcgcttc ctgctccaat cggtgcatga
    15661 cttgtggaat caaactgcgg gaagggactt ccttggcaac ctgctcgtct tgaagcatgc
    15721 gaagcgcgtt caggagcgtg aggaaatcga tggccggttc cggttgtgga ggcgcgccct
    15781 gctcaggacc aaggtccagc gtaatgccag tggccatggt gacggacgcg atgacgagac
    15841 agaactgttc acggaccgtg ccgaaatcct gcttggtcgc cgtcgtccgg tctttcaggg
    15901 tgtcgatgaa gggaagcagt gcgaaggtag cgcgttcgac atcgggaagg ttcaccgtgg
    15961 cggcagaacc accgagactg gtgatgcccc tcaccacggc atccaccagt tgcgcatgcc
    16021 gatcggtatc cggctggctt tccagttcgg tcagggcgga atggatttgg atgagccact
    16081 cttgcgcctc ttgaccgaac agttcaatca gttctttttg aaaatcgtct tcggtctgtt
    16141 ctggttcggt cgacacagtg gcgccctgcc tctgacccat gcactcgcct gaacatacgg
    16201 acgagtgacg gcctcctagc gttttccatt accgagttgg gaggccagcc ccgcgatctc
    16261 ccccagcttc ctcgccatat cttcgaaccg gcgagtcgac tgctccgcca tgtgctcggc
    16321 ttccatcgcc cgtttctcga gctgcatcga acgctcctgc tcgctgacca gggcgagatc
    16381 cagctcactg gcacgttgcg ccgtcgcttc caactgtttg acctgaacta acagcgccgc
    16441 cgcagcctcc cgttcagccg agagcgccgc gtccagctcg gtgatctgag catgggcttt
    16501 cgcacaggcc tcttccagcg ccggcacctt gtctgcaacc tgttccagct cgacacgctc
    16561 ccgtcgctgc atttccgctt gctcacgttc agcggccaag gtcgtttcga gctcgtgcac
    16621 gcgcgtttcg acctgctcaa gaccggtaat ctgctgcacc aattgattcg ccgcttcccg
    16681 ctctgcctcc agatgggcct ccaattccac gatgcgcgcg gcatgacggg ccccttccga
    16741 aacctgttgg gacaattgct gggcacgatg ctgctcatcg gccagagccg tctccagttc
    16801 gccgatccgg tgcatctgtc tcgagactcc atccaactgt tctgcgagag cggttcgctg
    16861 cgcccgctcg tcggctagac tctggtccaa tgtgtgtgca tgtgacgaca gggctgcaac
    16921 ctgctcgtgc tgctcatcca acgcacgctg gatgtgcccg atctgctggg tctgctccag
    16981 actccgccga cgctcctcgt ccaactgcgc ttcgagttcg ccgatccgcg tgtcacgttc
    17041 cgccaggatt gcatgatgat gctcatgatc atgcgccggc gtagccggct cagcggcatg
    17101 gaatgcaggg gccgcatcca ccatcgtgtc gcgggtcatc gtcgccggta tggcgtgagg
    17161 atcgtcaaga ctgctcgtga tcggctcgag cgacggccga tgctcctgag tgtctacgcg
    17221 ggcctcttcg agttcttccg gcacatgaat ggcgggtgcg gccgcgacct ctgccacgat
    17281 agatggttcc acggccacgt gctgctgcgg cagttccgga accggagcag gcttttgtgg
    17341 gcgcttcgtc aacaggtcca gcacacggtc cttgagtgag gagccctgaa acggcttctt
    17401 caagaccccg tcggccttac aggactccgc ttgtcgggac acttcatcgt tcacgatacc
    17461 gctgatcagc agcacgggaa gatccgcgag gtttgcctgt ccccgaatgt acgcacagac
    17521 ctcatagccg ctcttgtccg gcatgatgac atcggacacg atgaggtcgg ggcgatcctt
    17581 acttaacagc gccagagcct cagatccgtt cgccgcaagt gtcaccccca tgccggcctc
    17641 ggtcaggaga cgctcggcca ccttacggac tgcgatactg tcgtcggcaa tcaagatctt
    17701 tggcatgtcg ttcctctaca caaggcaagg ctcatcacgc ggggcggttc gatgagaccc
    17761 ccaaggttgt caggttgatg ataggaagtt cttcttcgcc ggcgatgcac gttcccgcga
    17821 tgtcgggatc gttgccggcg cgatactgca ctgccgagcg tgccaccatg tgcaaggaag
    17881 gaacctggga atcgatacag attgccagcg gtccatccac atgtttgaca accagacaca
    17941 gcggttcacc atcctgcagg gaacgcttga acttcgccgc caggtcgaat accggaagac
    18001 atcccttctc ctgtcggacg agagcgcgca caaacggcgt atcgctggga accgccgtcg
    18061 agcccgtcca cggcatcacc ccgccgatct cctcggttct ggcggccaac cgttgcccgc
    18121 ccaccgaaaa caccaccatg tgctgcatcc gcgcctcggt cgcgcctttg ctacccttgt
    18181 gcggttgtct gcctagctga gtacgtagca tggtcgatcc ttgtgaccta cttggccgat
    18241 ccggccgaca tcgccgacgc ctcttccaag gccgccgaag gcaggcggag tctggaatcg
    18301 aattcgcccg gccggcgctc cgacacccga agggcaaaca catccaagac attggtccgg
    18361 gaggggagct cgagcaccca aaacggattg gcgatcagcg cgatatacga cgactcaaca
    18421 aacaatccga gtaatcgctc ccgctcccct ccccgaaact gcgcagggag cggctggatc
    18481 agcgtccgtt cgacatcgat gaggcccacg accttgtcca ccgcaaagga ccgcgatcgt
    18541 tgctcattgg catagagcac gatccgggtc tcagccgtga cgcctttggc ctggatcgtc
    18601 aaacgacggg cgagatcggt gcgttcgtag gaagcattgg cccaggtgac atgcccgtcc
    18661 tgtcctccct cagccggagt cacaatgccg cgaacccagt gggagggaaa ggccacatgc
    18721 gtggggccca tgagcgccac gagaaaccgg accgccagcc cgctcgacac cactgctgtc
    18781 ttatgtcccc ggagccccat gcctgtcctt cattctgcca gtaacggtac gaatcgacaa
    18841 aacgttccta ttttttcacg acgctttcgc ggcctgtcca cttgcggact tcctggatca
    18901 gggcgcgttc ttccaccggc ttggcaatgt accccgaggc ccccacactc acggccatct
    18961 ggcgatgctt ttcacccgcc cgcgtggtca tgacgagaat cggtgtggtc tgagtctgcg
    19021 gcctggcacg aagcgcctga atgacttcaa agccgttcaa cttcggcatt tcgagatcgg
    19081 tgatgatgag ctggtactgc tgcaccaatg ccttgcgaca gccttcttct ccgtcggtcg
    19141 ccgtttcgac gatatacccg gccgactcca gcatgcgtcc gacaaacttt ctgatgctga
    19201 gcgagtcatc gatcaacaag agcggaagct gcgtatccgg aagctgcggc gaagcgggct
    19261 gcgccggtcc ctcaagcatc ggcgaggagg cggggttttg aatgaccgac atggcctcat
    19321 ggtattcgcg agtcgtcaac cggctgacgt ccagaaccag caccacacgc ccctcgggat
    19381 cgatagtcgc gccaccgaac acggaccgtt catacggctt cagcccaccc aatgatttaa
    19441 tgacgatttc ctgccggccc agcaattcat ccaccgcgca accgagcgcg ccggtcgagg
    19501 ttcgaacgac gaccacggga gtcgctccct caatggtgcc ggcatcccgg cgaatgaggc
    19561 tgccgagcgg atagacctca atggcttcgt cgccgatctg cagcaccgat cgatccccca
    19621 tctgctggat cgtcgacgcc gtcgacattg tgacctcacg cacgctcgga agcggaatcg
    19681 cgtagcgctc tttcccgacc cgaacgagca gcgcagttgc gatcaagagg gtgagcggca
    19741 aatgcatcgt gaacttggtg ccctgcccgt agacggattc cacctcgatg tggccgttca
    19801 tcgtctcgat gacccgcttg accacatcca tgccgacgcc gcgtccggcc tgatccccga
    19861 ccgcctcggc agtggaaaat cccggcagga agatgaactt gatgatttcg ctctccggca
    19921 atgagtccgc gacatccgct cgtaccagac ccagcttgac cgccttggcc ttgatcttga
    19981 cgatgtcgag ccccgctccg tcgtcttcga cttcgatgag caccgagttc cctcgatgcg
    20041 cggcgtggag atagatcgta ccggccgccg gcttcccttg gctgcgacgg atgcccgcag
    20101 gctcgatccc gtggtacacc gcatttctga ccagatggac aagcggatcg acaagccgct
    20161 ccacgacacc ggtgtcgatt tcggtatgtt cgccggacgt gaccaaattg acttcttttc
    20221 cggtggcccg ggccatctca cgggccgcgc ggcggaagcg cgtgaacggc gtcccgatcg
    20281 gcaccatgcg ggcgcgggca atttcatcgc gcatgcccaa ggtcaactgt tgcagcgacc
    20341 ccatgtcgtc ctgggcgcga tggatcgatc cgctcagctg cgacatcgat tcggtgatgt
    20401 ccgcggtgac ctcgctgatt cgccgggcca ggatgttgaa gtcgtcgtac ttgtcgaatt
    20461 ccaggctgcc gaaatcactg acgcccggat acgcctgcgg cgcgccttcg ctcgaggacg
    20521 cgggagaggg ctgaaaggaa aaggtatgtt tatcttcgaa ggtgcgtacg gcatcggtca
    20581 gccggttctt gttggccaag acctgtgtgg ccaattgatc caacgtgcgc agccgctgct
    20641 ccagccggcc gcgatcaata accaattcgc cgaccagatt gaggaggcgt tccaagcgat
    20701 cacggctgac ccggatcacc tctcgctctt cgccggtctt cccatcgacg cctttggccg
    20761 aatcctgtcc gtcggcttcg cgaatttctt ccaccgaggc gccttcggca atcgatgaga
    20821 cgatggggtg atcttggtcc ggtgccggct gagcctgtcc cagttgcttc aactcctgca
    20881 aggagttcgc aaaccgttgt ctcgttcgcc ccaccatgga tacatcccgt cgcatcaagg
    20941 cgcgaatgac atcgattgag cgcaacagga catccgtgtg accgggaagg agcaccagct
    21001 gcccttcgcg aacggcgccc atgaagtctt caatgtgatg agtcagatca ccgatcgcct
    21061 gaaagcctac ggtataggcc gagcctttca gagtatgggc tgtgcggaac agttggtgga
    21121 tcgtgtcggg attgcccgcg tccttatcga ctcgcagcaa gtcggcttcg aggctttcca
    21181 aatattcctg cgcttccggc gcgaaatagg acatcacctc agaatcgagg gccgggatca
    21241 gataggcttc ggagagctcc tgggagtccg cctcgctcat ggagtgcaca gcggattcgg
    21301 gaccggatgt ggcggcaggc acggactcct gcaccgactt ttcttcaggc cgcctcgtct
    21361 cagcggcagc cgctgcagac tcaccctggc tcacgccctg ctccaattct ctgagcttcg
    21421 tcctgatgcg aggcacctcc tgacgcaagc gcgtcagtga ttgagcgtcc cgcgccatca
    21481 gggaacggat cacatccacc gcctgccgca gaacggcgat ccaatgcggc gcgatcgtga
    21541 cagcgccctc gcgaacggcc atcatgcagg attcaatcgg atgagagaga tctcccacga
    21601 cctcaaatcc aacggtatag gccgaccctt tcagcgtgtg cgccacccga tacaattgat
    21661 gaatcgtgtc ggcatttgcc aagtcggagt ccaaccgttg gaggagggtc tgaatcgtgc
    21721 tgaggtattc ttctgcctcc ggcgagaagt aggacagcac ctcttcgtcc aggacaggaa
    21781 tgaggtactc atccgccggc tgatcggaac gctgcacaga cgctgccgac gggtcgctcg
    21841 aaacggccgg catcaagtcg gagtggcgcc ggacaaatct ctcaaccacg gaggcatctt
    21901 cccctccccc acgcccgatc tgctggacct gccctcgaaa ggacgcggca atctcgcgca
    21961 tcagttgcat ggcttcgggc cagcgcgccg ccggaatctc ctgcactcgt tcgagcgtat
    22021 cttcgagtag ggccccgagt tgcccgagcg ccgggaaccc gtagaggagc gccgcgcctt
    22081 tgagcttgtg gccgaccgtg tgaaactcgg cgacgtcgcc cggttccgga tgcgccttcc
    22141 catcgggatg caacgctttc cagaagcgcc ccatatcgtc gccggcctcg gccacgaaaa
    22201 tgtccagcaa ctggtcgcga tcgaaatcag cgctcattca cccactcagc atccgagcgg
    22261 aggtcggtca ccgctcttca agaattctct tacgcgagct tgaactgagc gaccgaagag
    22321 ttgaggcctt cggccagttt tgccatgtct tcgatcgtga gtcgcgtcga gtccgtctgc
    22381 ttctgcgtcg ccacggcgcc gccggtgaac tccttaatgg ctcgaccgac tttttccgtc
    22441 gaggcggtct gttcggacgc cgcgctcgcg atgttctggg ccaactcgga agaccgtttc
    22501 gcaatgtctg aaatttcagc gaacacgtcg ccggtccgga gagccgaagc ggatccggct
    22561 tccaccgcct gcgtttcatg ctccatggcc accacggcat cctgcgtttc cgtttgaatc
    22621 actttcacga ggtcggcgat ttcacgtgtg gcttgcgtgg agctttccgc cagctttcgg
    22681 acctggtcgg cgacgacggc gaatcgtgcg ccggcctcac ccgcgccggc cgcctcaatg
    22741 gcggcgttga gcgcgagcaa gttcgtttga ttggcgatat cgcggatcgt cgacacgatc
    22801 tgtgaaattt cgagtgaacg gtcaccgagg cccttcacct gcttggacat acgttgcacc
    22861 gccgaccgga tgctctgcat gtcgcgcacg gtttcctgca ccgcgacgtt gccgcgttcc
    22921 gtggccgaca gcacctgctt ggcggagtcg gaggatgcgc cggccgtagt ggccacttga
    22981 cgcataccgg cggccaactg ttcgaccgcg cccagcgtac gcacggattc ttccgcctgc
    23041 acccgggctg ttccggacat ttgaccggct gaatcccgca gggtaccggc tgacttgttc
    23101 acccgatcgg ccgcctcgcg cacctgcttc aacaattgtc cgaaacgcgc gatcatgagg
    23161 ttgaacccgt ccgcaaggtt gccgaacatg tcggccgtca cttcgcctcg ccgggtcaag
    23221 tctcctttac cgacttcgga gaccagcacg aggaattgca tgagccgttt ctgcatcagg
    23281 tcgcgctctt cctccgtcga caccagggcg gtaatacgat cgagcatgga gttgaacgcg
    23341 gtggccatct ggcccaactc atcgtgcgac tgaatcttcg cgcgggcctg cagattgccg
    23401 ccggcggcct gcgtcgcgac attggcgatg tggctgatgc tccgggaaag gaacgtcgct
    23461 aacagatatc cgacgaacaa tccgagtgcc acggcgacca aaccgccggc caccagaatc
    23521 agggtaccgt tgctggccag gccttgcgca tcatcgttga ggtccttggc gacggcctca
    23581 atggcagtga cctgctcatt gtgacgtttg acggcatttt cgaatgaggg cgtcaaattg
    23641 accgttaagg ccaggatacc gagggcccgc atttgctccg cttgcgtcga tgacaggacg
    23701 ttacgatcga agctatccgc catggcgctc agtgcgccgt cggcatcctg aaagtatttt
    23761 ttcaatgccg gctcaaagag ggtcagatcc ttcgcctcgt cccggcccga tcgcgatacg
    23821 cgcagcgtcg tgctcttata gttgttcacc accttgtcga tctgggcctt caggggcggc
    23881 aacttggtca cttcctgggc gaagtcgcct tgtttggtgg cggacgcgat atccagcaga
    23941 atctggtgat gtttggtcaa cgcggttccc atctgggcga actcagccaa cggcaccgta
    24001 tagtccgcat acaccgtttg cgactgggtg ctgagttggc gcagggtgaa cacaccgacg
    24061 ctggccatga tcatgatgat gacactcacg agaccgaagc tgaccagcag cttcgtcttt
    24121 gttttcatgt cctggaatcg attcttgacg ccttgcccga tcatggtgcg ctcctccttg
    24181 ttctgctttc ctcaacgtcc ggtggccggt cgcacaatct tcgatgaagc aggtccgcga
    24241 tgactcgcgc gcctgctgtt tacgcgatcg acaatatcgc actccctccg tcaacttgtg
    24301 caaagacctg cggcacctcc agcactccac ccagacgttc atccaggcga agcacagccg
    24361 agacacaagg acgcgccccc gccggtcccg cttgcatggc cggcaacagg tgctcacgac
    24421 tgacggtgtg aatttcagga acgtgatcga cgagcacacc cacctgtctg gtgccatggc
    24481 gaatcacgac cgcgaagggc aacgcggtct cggtcacggg caggcccagg cttccacgca
    24541 gatcgaccag ggtaacgacc gttccccgga gattggtcac tccggtcaac aggctcggca
    24601 tgctgggcac gacggtcacc gcttccacct caaacacttc actgacgtgg cggagatcga
    24661 tggcgaacaa ctcgcccccc agcgtgagga cacacatacg cagcgtgcct tcaatcgacg
    24721 cggcaagaga cctcgatggg ctggagggtc cgtgagccgg agaggagtct gaggagtgag
    24781 gctggatcgt tggcacgtcg ctcatggatt agttcaacgc ccgcttgact gcggcgacca
    24841 gatcttcagc tttaaacggc ttcaccacat acccattggc accctgctgt tggccccaga
    24901 atttgtcgct ttcctgcccc ttggacgtgc agaggacgat cgggatgccc ttgaagcggt
    24961 catcgctttt caggtcgcgg caggcctgga acccgttacg cccgggcatc acgacatcca
    25021 gcacgatcag gtccggctta tccaacgcca gtttgtcctc gagtttgtcg gtgttcggat
    25081 acgacacgac tgtgtgattg gcagacttca aatagccttc gatcaactgc agttcggcgt
    25141 acgaatcatc gacgacaacg atcttgctca tgagtacgtc cccccttcaa accagctaaa
    25201 tgtgtgcgcg ataaagacat ttgatccggt tgcggctatc gcatcgggat cacgatgctg
    25261 atcgcaccgg ccagatcgcc ctcgtgccat ccttctttct tggcgccggt gatgtcgcgc
    25321 tcccctttcg gatcgccatg gcaactcagg cacgtgctcg cggcatactc cggatccatc
    25381 atgcgcagta ccggcttccc gtccaacatc gtggtcttgc tgtacggctg gcctttcgga
    25441 tggcgcgtgt cggcaaaaat tctcaacact tccgactcaa actcgtccgg ccgattgccc
    25501 ggaaaccgat agtcggtgcc cgtcaacttc acacgaattc cggtcttccg atagaaccgt
    25561 tcgccggact tgcgggcgaa gacggcgggg ataatccctt tgaagccgac gccctgctta
    25621 ttgatgacgg gctgcgcctc gtcgatgact tctttttcgg attccaccat ggcgagaaaa
    25681 agcggcgctt gcggaaccgt ggcgggccgg ctcagatcga tcttggtggc tttgcgaaat
    25741 cgttcgatca cctgattgga cacaaagtcg ccgctaaacc ctttgtcgcc cttgctggcg
    25801 tcattgatcg tggcttggta ttcggaaatc accccacgac cgacttgcaa caacttgatg
    25861 agcaactcgg ccgtttccac ctcggtattg gccgacgcgg gagcagccgc gcccatgcag
    25921 gtcacggtca atgcagccat cacgattctc ttgatgatat gtttcgccat gccgtctcct
    25981 cctgcctgcc tcgtagcgcc cggtcaaccc aattccccta agtcagccaa tcccttgtca
    26041 aagatagcgc aaaattcttc gttgacaatt tcatcgacgc atggaaccac gccaggaccc
    26101 tgcagcccag gcagacgttg tgcacgatcg gtgccaaaca aaacaattag ttaggattgc
    26161 ggagcggaaa gaattcgaac cgatgaagtc ggcgattttg cggatgatta tgtgtggatg
    26221 gtctactata cccactatgg tcagctaggc tgacatgggt gcgtaattgt tcacgatgga
    26281 agtcggagcg ccaaagtttg gtcgcttttc gagggaagcg tcaaagtccc ttcagaactt
    26341 cgacgaaaag aggattgcgg ctggcctaga ccgtcccacc ttgacgaagg agcgccagga
    26401 tcggggtaag atcaagatgt tgttcgacgt gatcggccca tcgatccatc gcaccggatc
    26461 gctgtcggtt cacc
//
LOCUS       DAOQRM010000016        25759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1187_length_25759_cov_14.638928, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000016 DAOQRM010000000
VERSION     DAOQRM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25759
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1187_length_25759_cov_14.638928"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /gene="tenA"
                     /locus_tag="PKK81_02880"
     CDS             complement(<1..653)
                     /gene="tenA"
                     /locus_tag="PKK81_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HNV31844.1"
                     /translation="MSFSNHLRKLAQPVWDAQLTHPFVVALGKGTLAERKFRYYILQD
                     ARFLADLARVFAAGSLRAPDSESALRFAKLAEETITVERSLHENYGKRWKLSPHDMLN
                     EPMAPTNYAYTRHMLAVAQGGTATEIAVVALPCAWIYCVVGKHFLKNGPPSAKHPYRD
                     WLMLYASPEFEAVQEWMRARVDTWARTAGHEEKKRMEQAFLISSKYEWMFWEMAWNE"
     gene            complement(778..2181)
                     /gene="sthA"
                     /locus_tag="PKK81_02885"
     CDS             complement(778..2181)
                     /gene="sthA"
                     /locus_tag="PKK81_02885"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNV31845.1"
                     /translation="MAHYDLLVIGTGPAGQKAAIQAAKLGKKVGIVERKRVVGGVCTN
                     TGTIPSKSLREAALYLSGFHQRSLYGASYRVKQDITMEDLTFRANHVINREIEIIQNQ
                     MTRNNVDLWFGTAGFIDPHRLRIERSDDLVEHTADFVVIACGTVPARPSHIPFDDHSI
                     IDTDGLLTLKTLPKSITIIGGGVIGAEYASILATMGIHVTLIERRPRLLEFVDQETIE
                     ALQYHMRSIGVTLRFNEEVVSVERQPQEQVIVRLKSGKEIAATTVLYSVGRTGASATL
                     NLEAIGLAADDRGRLKVNEHYQTTVPHIYAAGDIIGFPALASTSMQQGRHAACHAFGI
                     PCQTQSELMPYGIYSIPEISMVGRNEDDLTKNGVPYAVGIARYREIARGQIIGDELGM
                     LKLLFHNKTRQLLGVHAIGDGATELIHIGQTVMAYQGQIDYFIEAVFNYPTLAECYRV
                     AALDGINQLPRPWPPRV"
     gene            complement(2353..3039)
                     /gene="tpx"
                     /locus_tag="PKK81_02890"
     CDS             complement(2353..3039)
                     /gene="tpx"
                     /locus_tag="PKK81_02890"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNV31846.1"
                     /translation="MASPQTRMIPGASTSHFLAALLYLGLSGCGTLPSSGHSDFSYKN
                     MPVADGSAVAGEGNNVLFQGKPLMLSGMGIKVGDKLRDVKLTQTDLSMVPINDTKGKG
                     KVRIISIVPSLDTKVCEQQTHYLSEKNMGLDRMVELITISIDTPFAQKRFAEEAKIAN
                     VTFLSDFRAADFGKAHGLLLKDPHLLSRALLVVDKDNKVRYLQITPELAQLPDIEEAF
                     RFARKLVTAS"
     gene            complement(3110..3994)
                     /locus_tag="PKK81_02895"
     CDS             complement(3110..3994)
                     /locus_tag="PKK81_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV31847.1"
                     /translation="MSGCAAPPEIPRWFDGFQRFPIRTASVNGHRIAYLDEGQGPPLI
                     LLHGYGGSMWQWEYQQLPLSRQFRVITPDLIGSGLSDKPPLDYRPEELIESIRGLMDA
                     LGLPTATLIGNSMGGGVAIGMALTHPDRVSRLVLIDSLPDHVRERLVSPLMQRALNTS
                     VPAWLARFGALFVGNRTMEAVLKEIIYDHTLVTPAVLDRSNRNRQRKDMITPLMSLRD
                     SLPLWEQHFAPRLKEIRHPTLILWGEQDRLFPPQVGRDLQTTIPQARLIMIPEAGHIP
                     QWEQPQVVNRHITEFLQP"
     gene            complement(4078..6066)
                     /gene="oadA"
                     /locus_tag="PKK81_02900"
     CDS             complement(4078..6066)
                     /gene="oadA"
                     /locus_tag="PKK81_02900"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HNV31848.1"
                     /translation="MATKKTKKTAPKKSPAKKNVPAKPSRPAASRAVQVTQPASPEFR
                     VTPAPGKKLLMTEVALRDGHQCLLATRMRTEDMLPIAQKLDAVGFWSLEVWGGATFDT
                     CLRFLKEDPWERLRALRAAMPKTKLQMLLRGQNLVGYRHYADDVLEKFIERSAFNGID
                     VFRIFDALNDVRNLERAIREVKACEKHVEAAISYTTSPVHRLDGFVTMGKRLEDLGAD
                     TICIKDMAGLLAPADAYRLVKSLKAAVRVPIHLHSHYTSGMGTMSALMAVMAGLDLLD
                     TSISPLAGGASHPPTESMVAALRGTPYDSGLDLEDLQPIAEHFRNVRRKYRQFESDFT
                     GVDAEILTSQIPGGMLSNLAAQLAEQNALDRMKEVMDEIPRVRKDMGYPPLVTPTSQI
                     VGTQATLNVLTGEQGERYKVITTETKNYFLGLYGRAPGPLDKEIMARAIGDEEPVKGR
                     PADRLESEFEKLKKDMPESATTLEDQLSFALFPAIARDFFEARERGDLRAEPLEPTET
                     KGPAVAHDLHLAPAEFNITVHGENYHVVVSGSGRTTDGRKPYYIRVNDRLQEVSLEPL
                     QEVLAGVPESPGAGSTSKPKRPRPTKPGDVAPPMPGRVVKVLVTDGAQVKTGDPLLII
                     EAMKMESQVPAPMDGRVTAILVVEGDSVKIDETVIQLE"
     gene            complement(6138..7556)
                     /gene="accC"
                     /locus_tag="PKK81_02905"
     CDS             complement(6138..7556)
                     /gene="accC"
                     /locus_tag="PKK81_02905"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNV31849.1"
                     /translation="MFRKILIANRGEIAMRIIRGCRELNIATAAIYSEADSSGIYVKK
                     ADESYLVGPGPVKGFLDGKQIVEIAKRIGADAIHPGYGFLSENTKFARLCQTSGITFI
                     GPSPETIDLMGSKVKARQIAQQAGVPIVPGTEGGVTSVDEALAFAHQINYPVMIKASA
                     GGGGRGLRVVRSDQELRENIDVASREAQAAFGDGSIFIEKYIERPHHIEFQILGDKHG
                     NIIHLGERDCSIQRRHQKLIEIAPSLILTPKLRAQMGEAAIAIAKAVHYDNAGTVEFL
                     LDHEGHFYFMEMNPRLQVEHTVTEQITAIDIVRNQISIAAGKPLEIRQKDVTLQGHAI
                     QCRINAEDPRNNFMPCTGTITAYLSPGGIGVRIDGAVYRDYTIPPYYDALLAKLTVRG
                     RTWEEAVSRMRRSLEEYVLRGVKTTIPFMKNVMMEQDFQAGRFDTSYLETHPDLYQYE
                     ESEEPEDLVLAISAAIAAYEGL"
     gene            complement(7730..>8124)
                     /locus_tag="PKK81_02910"
     CDS             complement(7730..>8124)
                     /locus_tag="PKK81_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNV31850.1"
                     /translation="VPAGHAQEGAMVEGAGYTMFNTESIKGSDTSKLESDPVCDRSKR
                     PSIAKVEPDEAKPGEKITIKGENFGSKECFHGVTFSAASKEKIDYRFVNETTIEAVVP
                     EAKAGMSFIIVVAGGGSAQSKPLLIQPK"
     assembly_gap    8125..8224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8225..8721)
                     /locus_tag="PKK81_02915"
     CDS             complement(<8225..8721)
                     /locus_tag="PKK81_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNV31851.1"
                     /translation="MHTRISGFVCAALLCLTGLPASAEVAGSMAIAGHGPEQRVIESL
                     AHAFEKANPRAYIDVVWDDNSKPLDLVKTKQAKIAVTGTAEDGLRSFQIAWDGIAIMV
                     HRSNFTKEVTKQEVAELFSGKYKVWADLGGPDTKVLLIDRPRNENNRDAFEQQLGIAG
                     KIPEGA"
     gene            complement(8819..9136)
                     /locus_tag="PKK81_02920"
     CDS             complement(8819..9136)
                     /locus_tag="PKK81_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV31852.1"
                     /translation="MPVYEYRCEQCTHQFEATQSVHARPEDTVCPQCNKIGATRMLSA
                     FASKVKGSHKPGFEEIKAYDMLNERMDRFSKLPPAMGKRIEASPDFMAGAGGPAPTEG
                     HDS"
     gene            9295..9471
                     /locus_tag="PKK81_02925"
     CDS             9295..9471
                     /locus_tag="PKK81_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31853.1"
                     /translation="MPLLIRKYKGSGGVMQEERIDDPDRIERYMRLFEEKDVKKLGTG
                     VKTVIEKDEWQLLP"
     gene            9621..9824
                     /locus_tag="PKK81_02930"
     CDS             9621..9824
                     /locus_tag="PKK81_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31854.1"
                     /translation="MVYWLHIARAIDRVTLHRDRCSEVPSGVSSSDYWQGGWFDYPDK
                     ERALAAMEQAGTSEERKCALCKP"
     gene            9931..10809
                     /locus_tag="PKK81_02935"
     CDS             9931..10809
                     /locus_tag="PKK81_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV31855.1"
                     /translation="MPQFALLLTTFVWGATFPATKAALEQISPLSFLFLRFLLGMTVV
                     FAVLLFFRRPLIRDTSMMRASLIATAWLFIGYVLQTVGLRFTTASNSAFITVLYVVFV
                     PLYLLRLGLHTWVSNGIALAGLWLLVRPTASANLGDLLTLGSAAAFAAHMVCLERYTR
                     VADSVSLFAWQLVLMTVAMSGVMWWEQPTLAMFEPSRVLAVGLVVTGILATGAFAVQM
                     WAQRLLPAQQVALLFAAEPAVAAWLAWYFLGEHLDAQGWFGSAMILGGVLLGSWVTGE
                     SSPAQQESVTARSEGG"
     gene            10811..11281
                     /locus_tag="PKK81_02940"
     CDS             10811..11281
                     /locus_tag="PKK81_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31856.1"
                     /translation="MAQKFGNNRWVQEGFLDNRRPGTVVGRMTFAVLGAVDFYLVGDC
                     RGEIAGKVIRFKNSRFVDEDLAGQVLGDVEIPQIGDASLISFDPHPHLVPHPYIEWFS
                     MRKNHYRIELVPEDAWIATEEELAAIDPVSLEIRDRLALQYGRKAASADESEWV"
     gene            complement(11329..12345)
                     /locus_tag="PKK81_02945"
     CDS             complement(11329..12345)
                     /locus_tag="PKK81_02945"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNV31857.1"
                     /translation="MSSDLQPLTLPENGELSKTSDQPREQPASDGPWITIIRWFDSWA
                     GIEKMQTDTAPPTVDWLRAIPLIIVHLLCLGVFIVGWSWVAVGVAAGFYFIRMFAITG
                     WYHRYFSHRTFKTSRACQFAFALLGASCAQRGPLWWGGHHRHHHIYSDKPEDTHSVRQ
                     GGFLWAHIGWISSKKFFPPRLKSIGDFAKFPELVFLDRFDTLVPTICGFGMFGLGKLL
                     EAYAPSLGTNGPQMLIWGFFVSTVALLHGTCTINSLSHVYGSQRYKTGDDSKNNFILA
                     MITLGEGWHNNHHYYAASTRQGFYWWEIDITYYMLRGLQALGLIWDIREVPAHVREGK
                     NKLA"
     gene            12710..13735
                     /gene="hemE"
                     /locus_tag="PKK81_02950"
     CDS             12710..13735
                     /gene="hemE"
                     /locus_tag="PKK81_02950"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNV31858.1"
                     /translation="MNDRFLKACRREPVDCTPVWFMRQAGRYMVEYRRLREKHSILEL
                     CKMPELAAQVTLQPIDRFALDAAIIFADILLPLEPMGLSLEFAEGEGPIIHNPVRDRA
                     AVDRLKVIDDTELQYVMDAISLTRKMLAGRVPLIGFAGAPFTLASYAIEGGSSRNYIH
                     TKQMMYREPETWHRLMDKFARVITGYLRRQIKAGAQAVQLFDSWVGCLSAGDYAEYVM
                     PHVQLIFEGLKHEGVPLIHFGTGTTAILKAMRQAGGDVIGIDWRIPIDEAWAMVGYDR
                     AVQGNLDPVTLFGPISEIERRVTDILRRAAGRPGHIFNLGHGILPNTPVENVAATIDL
                     VHKLSTR"
     gene            13738..>14632
                     /gene="hemH"
                     /locus_tag="PKK81_02955"
     CDS             13738..>14632
                     /gene="hemH"
                     /locus_tag="PKK81_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNV31859.1"
                     /translation="MSVTPQPVAVLLMAMGGPDCLENVEPYLQDVRGGRPTSPELVEE
                     IRERYRVTGGKSPVLDIAREVARALEQRLNRSGDARYRCYVGLRHWHPFIKETYAELL
                     DALPERIIGLCMAPQYSSLSIGAYMKKVEEARAELASETPISFVTSWHRHPLLIAAIV
                     ENIRRTLERFPAEVRAQVPVLFTAHSLPERVVAMKDPYPEEVQGTAQAVCVQLGTQPT
                     RFAYQSQGRSGEKWLGPSVEETLGELAQEGHHHVLVAPIGFICDHVETLFDIDIELKQ
                     LALTKGLQLERIPMLNASAPLI"
     assembly_gap    14633..14732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14733..16203
                     /gene="hemG"
                     /locus_tag="PKK81_02960"
     CDS             <14733..16203
                     /gene="hemG"
                     /locus_tag="PKK81_02960"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HNV31860.1"
                     /translation="LAAPPVARTPRSVVIIGGGISGLSTAFALQEQAAAADMPLTCTI
                     LDAAPVWGGKILTHRVGQLVMEAGPDSFLSQKPWGMELCRRLGLADQLINTNPVEKKA
                     SVLRGGQLHELPEGLVTFTPTQLGPFFRSGLLSWVDLARMGCDVLIPPRRSTDDESLA
                     SFFRRRFGRHACERVMEPLMAGIYAGDAEQMSLRATFPRFYELEQAHGSVIRGMMAAR
                     RARAQKVSGGGPRHTMFVTLKNGLADLVAGLTAQIQQAGGVLKAGVQAEALRVRSHQA
                     GRWMYDVICTDGTALSAEALVLATPAYVSAELVRPLTPMAAGLMDMIPYASTATISLI
                     YPAEAVGNRLQGFGFVVPRIEGRDLIAATWTSLKWPHRAPPEDVSVRCYLGGVGREAI
                     LQRDDEALVQCVREELASIVGLLATPHYVEVNRWNRAMPQYTLGHLDRLAQLEAALSR
                     FGGLVVTGAGYRGVGLPDCIRDGAETAAKLLRYLQAAPV"
     gene            16246..16677
                     /locus_tag="PKK81_02965"
     CDS             16246..16677
                     /locus_tag="PKK81_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31861.1"
                     /translation="MLNDQLVISDVTGPFREPREPVLSYDYSIQRATWAATHAVRVKI
                     AQAEELDYVKEKLLGTVTGSPGQQLMLNKFLSRKIGDQKVRIAEAEGWLKERGDVLVA
                     PFTGSLAHYFPQLEAWVQAEQDTLRAEIKNLVGLVASPPAV"
     gene            complement(17059..17673)
                     /locus_tag="PKK81_02970"
     CDS             complement(17059..17673)
                     /locus_tag="PKK81_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31862.1"
                     /translation="MSTEQDPYEEGKRAGLHPLIVLFGAILVMWLLINLAVPSGKQKQ
                     AAGPEISQVTTEDPDAAPVMYKVQSTLTEFNAVSIVVPAQATTSQVVGLLKQFKQARL
                     AQSLPSMLPATSPGHKLGDQAVADLYIFSDPKYATPEAVGTLARGAHAPGNLYPQAIP
                     FEEAMEHVLGHYRIDLNDTSNPDTGSLGFADDSGVHSKQYRRIF"
     gene            complement(17670..18575)
                     /gene="mazG"
                     /locus_tag="PKK81_02975"
     CDS             complement(17670..18575)
                     /gene="mazG"
                     /locus_tag="PKK81_02975"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNV31863.1"
                     /translation="MSARFDNVVAIMARLRAPGGCPWDRKQTHESLKPYLIEETYEAL
                     DTIDRQDFAKLKEELGDVLLQVLFHSQIGTEAGTFTIDDVLEQLSDKLVRRHPHVFGD
                     GATSQSSLNSDQVVHRWEDIKRAERKNAGKPDSVLHDIPQALPALLRAYQTQVRAARV
                     GFDWPDSPQGLAAVLAKVEEEIGELKHALTDAATRRSVAEPKPEPTPEMAAEMGDLLF
                     SLVNVARHIKVNPEESLRLATNRFTARFQFIEREAARSGRSVNDLSTVEMDHYWEAAK
                     LVESTAPAGTVPPLVPSTEPTRTHP"
     gene            18708..19136
                     /locus_tag="PKK81_02980"
     CDS             18708..19136
                     /locus_tag="PKK81_02980"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNV31864.1"
                     /translation="MGDDKDQGFVIRDRRGRGEDAPPPAQAATSPAAEPSAAAPHHDH
                     DADSHTHSGHLPVNFSSFVISMGSSALMLMGEQLDPQQPSMPLNLPQAKEIIDLLSML
                     EEKTKGNLTPDEQVVMKDMLYALRMKFVSLTSGKSTLHTP"
     gene            19341..21638
                     /locus_tag="PKK81_02985"
     CDS             19341..21638
                     /locus_tag="PKK81_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV31865.1"
                     /translation="MPESTDMTKLIPPGVLREKKPSTPASIESERRKRHVRPVWIVLV
                     LLLPCLALTFYYAQMAVPVGEESDSFLPSTGYAFVLLLVNLDLIGFVVLTLLLSRNLI
                     KAYFERRHRLVGSGFRAKLVAAFIGFSLIPTVLLALVASGLVNKAVDVWFNDQIEHVM
                     KDSYEVARMHHAGHVSLAINSARAISHEIFREELLLPEQRDLLVAAIARKRAEYATAG
                     IEVFSSKMETLTKSLDPEVPVAVLDLPIGQLVLQVINGKQELTSVQEAQTGRLVRAGV
                     PIASSIRRGEIDGVVVVDAYVPESLLGKMESIGRQYTEYKQMKAMKNPIKAGAYLLVA
                     VITVMILFSATWFGFYVARGITVPIQRLAEATEAIAQGDLSVRIEAKATDEIGTLVES
                     FNRMTADLQSSKTKVEEANVSLRQSNLELDRRRAYIETVVDTIAAGLLSIDRQGIITT
                     FNPSAERMLGLWADRFRGRSANEVFKEYKLDLFQSVYDRMLVDQRDNIALEGQLDLQG
                     RFLTIGVHCSRMKDESNKDLGFVLIFEDLSELIKAQKAAAWREVAQRIAHEIKNPLTP
                     IQLSAQRLRKKFQDKAPDFDRICDESTQVIINEVGSLKQMLDEFSKFARMPAPHMTMN
                     SLDDVVKEVVALYDSAHREVECVVTLDADLPPFNFDREQIKRVLVNLCDNGIHAMNHK
                     GHLWITTRYDTKQRRAVVTVADEGTGIAPEDQEKLFVPYFSRKKTGTGLGLAIVRRIV
                     TDHDGQIHAGNHQPKGALFTFELPV"
     gene            21671..23071
                     /locus_tag="PKK81_02990"
     CDS             21671..23071
                     /locus_tag="PKK81_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV31866.1"
                     /translation="MSASILIVDDEVSILNSLSSILEDEGYEVSVAKSGIEALKLCAV
                     SPPELMMLDIWMPDMDGLETLRRLRELVPQTQVMMMSGHGSIETAVKAIKLGAYDYIE
                     KPLSLENVTLRVKHALDQHRLEQENRTLRTKVERKFELIGQSLAMRQLKQIIETAGPT
                     NSRVLIGGENGTGKELVARAIHQQSPRANRPFVAVNCAAIPETLIESELFGHERGSFS
                     GATTMKRGQFEQADGGTLFLDEIADMSLSTQAKVLRALQEQQFTRVGGTKLIKVDVRV
                     LAASNKDLLKEIEKGTFREDLFYRLNVVPIVVPTLRDRREDIPLLVKHFLHVHAEEQG
                     LKIKQVSPEAMDVFIQYEWPGNIRELRNLIERLMIMVPGPVIEASHASVALQVRPQLS
                     ATQQAAGVQAPNSLLTRHYDSLRDARNAFEKEFIARKLREHHWNISRTAEDLQIERSH
                     LHRKIKLLDVEMRPEM"
     gene            23291..24328
                     /gene="purM"
                     /locus_tag="PKK81_02995"
     CDS             23291..24328
                     /gene="purM"
                     /locus_tag="PKK81_02995"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNV31867.1"
                     /translation="MTTYRDAGVDIDAGDEFVERIKPHVRATFRPEVMTDLGGFGGLF
                     RFQANRYQDPVLVSGTDGVGTKLKIAFLMDKHDTVGIDLVAMCVNDIAVSGAEPLFFL
                     DYFATGKLALKTAEAVVRGISDGCRQAGCALIGGETAEMPSFYAEGEYDLAGFAVGVV
                     DRPKMIDGRQIVPGDAVIGLASTGVHSNGFSLVRKVLFEKSRLTVESVMPELGGALGE
                     TLLTPTRIYAKQVLSLAEACPIKGIAHITGGGITENLPRVFPARCGARIRRGSWPVLP
                     IFQTIQDRGGVELSEMYRVFNMGIGLILVVAPEHVDRVMAKAVEAGDRAYHIGEMVAQ
                     STDEGVVEYVG"
     gene            24318..24992
                     /gene="purN"
                     /locus_tag="PKK81_03000"
     CDS             24318..24992
                     /gene="purN"
                     /locus_tag="PKK81_03000"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNV31868.1"
                     /translation="MSGKSPRLVRLGVLVSGRGSNLQAIIDAIEAGTLSAEVAVVLSN
                     KQDAGGLERARKHGAPAVWLDPKPYAGRPDSREAFDKAVLEVLQKHEVDLVLLAGYMK
                     IVTAVLVKAYENRMMNIHPSLLPSFPGLDVQKKAIDHGCKIAGCTVHFVTEGVDEGPI
                     IIQAAVPIREGDTPDTLAARILEQEHRIYPRAIQLYAEGRLRVEGRRVSVADAGPVAT
                     GFHNPG"
     gene            25065..25140
                     /locus_tag="PKK81_03005"
     tRNA            25065..25140
                     /locus_tag="PKK81_03005"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25098..25100,aa:Ala,seq:cgc)
BASE COUNT         4781 a         7066 c         8167 g         5545 t
ORIGIN      
        1 tcctcgttcc aggccatctc ccaaaacatc cactcgtact tcgagctgat cagaaacgcc
       61 tgctccatgc gtttcttttc ctcgtggccc gccgtgcgcg cccaggtatc cacccgggcc
      121 cgcatccatt cctggacggc ctcgaactcc ggcgaggcat agagcatcag ccagtcccga
      181 tagggatgtt tggcagacgg cggtccgttc ttgaggaaat gtttgccgac cacgcagtag
      241 atccaggcac agggcagcgc cacgacagcg atctcggtgg ccgtcccgcc ttgagcgacg
      301 gcgagcatgt ggcgggtata ggcatagttg gtgggagcca tcggttcgtt cagcatatcg
      361 tggggggaga gtttccagcg cttgccgtag ttctcgtgga ggctgcgttc cacggtgatg
      421 gtttcttccg ccagcttggc gaagcgcagg gccgactccg agtcgggtgc ccgcaaagat
      481 ccggcggcaa atacgcgggc cagatccgcg agaaagcggg catcctgcag gatatagtag
      541 cggaacttcc gctccgccag cgtgcccttt ccaagggcca cgacgaacgg gtgtgtcagc
      601 tgggcatccc agacgggctg ggccaattta cgaagatgat tcgaaaacga catccggttc
      661 ctcctttgtc gccgtcacgc gtacctcgtc gatcgtgaag cgtatctcgc gggctattcc
      721 cgatacagac tcctgccggt tcattcattg tctgcttcac gtttcacgaa cgacgcgtca
      781 gactctcggt ggccagggtc tgggtagctg attgatgccg tcgagggccg cgacgcggta
      841 acactccgcg agtgtcgggt agttgaacac cgcctcgatg aaataatcga tctggccctg
      901 ataggccatg acggtctggc cgatgtggat gagttccgtg gcgccgtcgc cgatcgcatg
      961 cacgccgagc agttgtcggg ttttgttgtg aaagagcagt ttcagcatac cgagttcgtc
     1021 gccgatgatc tgtccacggg cgatctctct gtagcgggcg atgccgacgg cataggggac
     1081 gccgtttttg gtcaagtcat cctcgttgcg gccgaccatg gaaatttccg ggatcgaata
     1141 aatgccgtat ggcatcagct cggactgggt ctggcaggga atgccgaagg catggcaggc
     1201 ggcatggcgt ccttgctgca tggatgtcga ggcgagcgcg ggaaagccga tgatgtcgcc
     1261 cgcggcatag atgtgcggca ccgtcgtctg atagtgctcg ttgaccttca gtcgtccacg
     1321 gtcgtccgct gcgagtccga tcgcctccag gttgagggtg gcgctggcgc cggtgcggcc
     1381 gaccgaatag agcaccgtgg tggcggcgat ttctttgccg cttttcaggc ggacgatgac
     1441 ctgctcctgc ggttgtcgct cgacggacac gacttcttcg ttgaaccgga gggtcacgcc
     1501 gatgctgcgc atgtggtact ggagggcctc gatcgtttcc tgatcgacaa actccagcag
     1561 gcgcggacgg cgttcgatga gggtgacgtg aatgcccatg gtcgccagga tcgaggcata
     1621 ttccgcgccg atcaccccgc cgccgatgat agtaatcgac ttggggagtg ttttcagggt
     1681 cagcaggccg tcggtgtcga tgatgctgtg atcgtcgaag ggaatatgtg agggccgggc
     1741 gggaaccgtc ccgcaggcga tgaccacgaa gtcggccgtg tgctccacga ggtcgtcgga
     1801 gcgttcgatg cgcaggcggt gcggatcgat gaaaccggcg gtgccgaacc agaggtcgac
     1861 attgttgcgc gtcatctggt tctggatgat ctcgatttcc cggttgatga cgtggttggc
     1921 gcggaaggtg agatcttcca tcgtgatgtc ctgtttgacg cgataactgg cgccatacag
     1981 gctgcgctga tggaaacccg acagatagag tgcggcctcg cggagagatt tgctggggat
     2041 ggtgccggtg ttggtgcaga cgccgccgac gacccgtttg cgttcgacga tgccgacttt
     2101 tttccccagc ttggcagcct ggatggcagc tttctgccct gccgggccgg ttccgatgac
     2161 cagcagatcg tagtgagcca tggtgtcttg attagtatcc gtgcttcggg aagcgtgaag
     2221 tgcaactcgc cggtaaacga aatctcgcac tcatagagaa gattccctgt ccttccgcgc
     2281 tatcagccat tcgccatacg ctcttctctg cattcgcgag agaggggcga cgcttcacgg
     2341 tggacgctgt ccttagctgg ccgtgaccag tttacgcgcg aaccggaatg cttcctcaat
     2401 gtccggcagc tgcgcgagtt cgggggtgat ttggagatac cgaaccttgt tgtccttgtc
     2461 cacgacgagc agggcgcgac tcagcagatg agggtctttg agcagaagtc cgtgcgcctt
     2521 gccgaaatcg gcggcgcgga aatcagagag gaaggtcaca ttggcgatct tcgcttcttc
     2581 ggcgaaacgt ttttgcgcaa agggcgtatc gatgctgatg gtgatcagtt cgaccattcg
     2641 atcgaggccc atgttcttct cgctcaggta gtgggtctgt tgttcgcaga ccttggtgtc
     2701 gagggacggc acgatactga taatacggac cttgcccttc cctttcgtgt cgttgatcgg
     2761 caccatcgat aagtcggtct gggtgagctt cacatcgcgc agcttgtcgc cgactttgat
     2821 tcccatcccc gagagcatca atggcttgcc ctgaaacagg acgttgttgc cttctccggc
     2881 tacggcactg ccgtcggcga ccggcatatt cttgtatgaa aaatcagagt ggccggagct
     2941 gggcaaggtt ccgcagccgc tgaggcccag gtatagcagg gccgctaaaa agtgagacgt
     3001 cgaagcgccg ggaatcatac gggtctgggg cgatgccata gaatatctcc atcgaagagg
     3061 aatgtaggct cagtgtgctc tgtcactgta tggccggttc ggcatgctgt caaggttgca
     3121 ggaattccgt aatgtgccgg ttcaccacct gcggttgttc ccactgcggg atatggccgg
     3181 cttcggggat catgatgagc cgggcctgcg gaatcgtggt ctggaggtcg cggccgacct
     3241 gcggggggaa aaggcgatcc tgttcgcccc agagaatcag ggtggggtgc cggatctcct
     3301 tgagcctggg cgcaaagtgc tgctcccaaa ggggcaggct gtcccggagc gacatgaggg
     3361 gcgtaatcat atccttgcgt tgacggttcc ggttggagcg gtccaatacc gctggtgtca
     3421 ccaacgtatg gtcgtaaatg atttctttca gcacggcctc catggtgcgg ttgcccacga
     3481 acagggctcc gaagcgggcc agccatgcgg ggacgctggt gttcagggcg cgttgcatca
     3541 gcggactgac cagtcgttcg cgaacatgat cgggcaaact gtcgatcaac accaggcggg
     3601 acacccgatc aggatgggtc agggccatgc cgatggccac gccgcctccc atggagttgc
     3661 cgattaaggt ggcggtcgga agtccgagcg catccatgag gccgcggatc gattcgatca
     3721 actcttcagg ccgatagtcg agagggggtt tgtccgagag gcctgacccg atcaggtcgg
     3781 gcgtgatgac gcggaactgt cgggagaggg gaagctgctg atattcccac tgccacatcg
     3841 aaccgccata tccgtggagc aggatgagcg gcgggccctg gccttcgtcg agataggcga
     3901 tgcggtggcc gttgaccgag gcggtgcgga tgggaaatcg ttggaagccg tcgaaccagc
     3961 ggggaatctc tggaggcgcc gcacaaccac tcatgaggac cgccgcgaga cacaaggtta
     4021 ggacgtgtga cgacgcgacg gcggtacacc gcacctgcca cgccgggagc ctcacggcta
     4081 ctccagttga atgacggttt cgtcgatttt gacgctgtct ccctcgacga ccagaatggc
     4141 cgtgactcgc ccgtccatcg gcgccgggac ttggctttcc atcttcatgg cctcaatgat
     4201 caggagcgga tcaccggtct tcacctgggc gccgtccgtc accagaactt tcacgacacg
     4261 acccggcatg ggcggggcga catcgccggg tttggtcggc cggggacgtt tcggcttgct
     4321 cgtgctgccg gctccggggg attctggcac accggccagc acttcctgca gcggttccag
     4381 tgagacttcc tgcagccggt cgttgacccg aatgtagtaa ggcttgcggc cgtcggtggt
     4441 gcggcctgag cccgagacca cgacatgata attctcgccg tgcacggtga tgttgaattc
     4501 agccggcgcg aggtggagat cgtgggccac ggcaggaccc ttcgtttccg tcggttccag
     4561 cggctctgcc cgcaggtcgc cccgttcgcg tgcttcgaag aaatccctgg caatcgcggg
     4621 gaagagggcg aacgacagtt ggtcttccag cgtcgtggcg gactcgggca tgtccttctt
     4681 gagtttttca aattccgatt caagccggtc ggccggtcgg cccttcacgg gctcttcgtc
     4741 cccgatggcc cgtgccatga tttctttatc gagcggaccg ggagcccgac cgtagaggcc
     4801 gaggaaataa ttcttggttt ccgtggtgat gaccttgtac cgctcgccct gttcacccgt
     4861 gagcacgttg agggtggcct gcgtgccgac gatctggctg gtcggcgtga cgagcggcgg
     4921 atagcccatg tctttgcgga cacgggggat ttcgtccatc acttctttca ttcggtccaa
     4981 ggcgttttgt tcggccaatt gcgcggcgag attggagagc atgccgccgg gaatctggga
     5041 cgtcagaatt tcagcgtcca caccggtgaa gtcgctttca aactgccggt acttccggcg
     5101 cacgtttcgg aaatgctctg caatgggctg cagatcttcg aggtccaatc cgctgtcata
     5161 gggcgtgccc cgtaacgcag ccaccatgga ttcggtgggg ggatgcgagg cgcctccggc
     5221 aagcggagaa atcgaggtgt ccaggagatc gagtccggcc atgaccgcca tcagggccga
     5281 catggttccc atgcccgacg tgtagtggga gtgcaggtgg atgggcacgc gaaccgccgc
     5341 tttgaggctc ttgaccagac ggtaggcatc ggcaggcgcc agtagcccgg ccatgtcttt
     5401 gatgcagatg gtatcggcgc ccaggtcttc caaccgtttg cccatcgtga cgaacccatc
     5461 cagccggtgg accgggctgg tggtgtagga gatggccgct tctacatgct tttcacaggc
     5521 tttgacttca cggatggccc gctccagatt gcggacgtca ttgagggcgt cgaagatgcg
     5581 gaagacatcg atgccgttaa atgccgagcg ctcgataaac ttctccagca cgtcgtcggc
     5641 gtagtggcga tacccgacca ggttctggcc gcgcaacaac atttgcagtt tcgtcttcgg
     5701 catcgccgcg cggagggcgc gcagacgttc ccacggatct tctttgagga accggaggca
     5761 ggtatcgaag gtagcgccgc cccagacctc caacgaccag aatcccacgg cgtccagctt
     5821 ttgggcgatg ggcaacatgt cctcggtgcg catcctggtc gcgagcaggc attgatgccc
     5881 gtcgcgcaac gcaacctcgg tcatcaacag ctttttcccc ggcgccgggg tcacgcggaa
     5941 ctcagggctc gccggctgcg taacctggac ggcgcgtgag gccgcgggcc ggctgggttt
     6001 tgcaggcacg ttcttcttcg cggggctttt cttcggggcg gttttcttgg tctttttcgt
     6061 tgccatactg gcggccttcg gcggcgaatc cgctcgcgta acagggttga ggtgttggtg
     6121 ccgagtggct ggggaggtca gagtccttcg tacgcggcga tcgctgcgga gatcgccagc
     6181 accaggtctt caggctcctc ggattcttcg tattgataca agtccggatg ggtttccagg
     6241 taggacgtat cgaatcgtcc ggcttgaaaa tcctgttcca tcatcacgtt cttcatgaac
     6301 ggaatggtcg tcttcacccc gcgcaggaca tattcttcca gcgagcgccg catgcggctg
     6361 acggcctctt cccaggtgcg cccgcggacg gtgagtttcg ccagcagcgc atcgtagtag
     6421 ggaggaatcg tataatcccg ataaaccgcg ccgtcgatgc ggaccccgat cccgccgggt
     6481 gacagatagg cggtgatggt gcctgtgcag ggcatgaagt tgttgcgagg gtcttcggca
     6541 ttgatgcggc actgaatcgc atggccctgc agggtcacat ccttttgccg gatctccaac
     6601 ggcttccccg ccgcaatgga aatttgattc cggacgatgt cgatggccgt gatctgttcc
     6661 gtgacggtat gttccacctg gagacgggga ttcatttcca tgaaatagaa atgaccctca
     6721 tggtcgagga gaaactcgac ggtgccggca ttgtcgtagt gcaccgcttt cgcgatggca
     6781 atggcggcct cgcccatttg ggcgcgcagt ttcggcgtca ggatcaatga cggggcgatt
     6841 tcgatcagtt tctggtgccg acgctgaatg gaacaatccc gctcgcccag gtggatgatg
     6901 ttgccgtgtt tgtcgcccag gatttgaaat tcgatatggt gcggacgctc gatgtatttc
     6961 tcgatgaaga tgctgccgtc gccgaacgcg gcttgcgctt ctcgcgacgc gacgtcaatg
     7021 ttctcccgca attcctgatc ggagcgaacc acacgcaggc ctcgtccccc gccgccggcg
     7081 ctggccttga tcatgaccgg gtagttgatc tgatgggcga aggccagggc ttcgtcgacg
     7141 ctggtgactc cgccttcggt gccggggacg atcgggacgc ctgcctgttg ggcgatctgt
     7201 cgcgccttta ctttgctgcc catgaggtcg atcgtctcgg gagacggacc gataaaggtg
     7261 atgccggagg tttggcagag ccgggcgaat ttggtgtttt cagagaggaa tccgtatccg
     7321 ggatggattg cgtcggcgcc gatgcgcttg gcgatttcca cgatctgctt tccgtccagg
     7381 aaacctttga cggggcccgg gcctacgagg taggactcgt cggctttttt gacgtaaatc
     7441 ccggaggaat cggcttcaga atagatcgcc gctgtcgcga tattgagctc acgacagcca
     7501 cggatgatgc gcatggcgat ttcgccacgg ttggcaataa ggatcttccg aaacatgatg
     7561 ggattgcgag aggcttggag ccgtagctca cctctgatcc gcctccaaaa agatcgcgta
     7621 acctagcata gggtcaagag agttgtcgag acgaaccggg acgaaaggat ggaccgtggg
     7681 aggggcggaa acgggcagga ggtgaggcac ctcctgcctg atgagtctct tacttgggtt
     7741 gaatgaggag gggcttcgat tgggcgctgc ctccgccggc caccacgatg ataaaggaca
     7801 tgccggcttt cgcctcaggc actacggcct cgatcgtagt ctcgttcacg aaacggtagt
     7861 cgatcttttc cttggaggcg gcgctgaagg tcacgccatg gaagcattcc ttgctcccga
     7921 aattttcacc cttgatggtg attttttcac cgggtttcgc ttcatccggt tcaactttcg
     7981 caatggaagg acgtttggaa cggtcgcaga ccgggtcgct ctccagtttg gaggtgtccg
     8041 atcccttgat cgactcggta ttgaacatcg tatacccggc gccctcgacc atcgctcctt
     8101 cctgggcatg cccagcgggg acggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnngcgccc tcgggaattt tgccggcgat gccgagctgt tgctcgaagg cgtcgcgatt
     8281 attttcattg cgcggccggt cgatcagcag gaccttcgta tccggtccgc cgagatcggc
     8341 ccatactttg tactttccgg aaaacagctc cgcgacttcc tgttttgtga cctctttggt
     8401 gaagttcgac cggtgcacca tgatggcgat gccgtcccag gcgatctgga acgagcgcag
     8461 gccgtcctcc gcggttccgg tcaccgcaat tttggcctgt ttggtcttca ccagatcgag
     8521 cggcttcgaa ttgtcgtccc agacgacgtc gatgtaggcg cggggattgg ctttttcaaa
     8581 cgcatgggcc agcgattcga tgacccgctg ttcgggcccg tggccggcga tggccatcga
     8641 tccggccacc tcggctgaag ccgggaggcc cgtgagacag agaagagcgg cacagacaaa
     8701 tccgctgata cgggtgtgca tgctgaacga gtctcctgtc agccttgagc cgctgatcgg
     8761 ctctctccgg cggtgttcta cgtgaacctg ttgcgcccgc ccggtaacac ggcttcgcct
     8821 acgagtcgtg cccttcagtt ggagcagggc ccccggcgcc ggccatgaag tccggtgagg
     8881 cctcgatccg tttgcccatg gcgggcggga gcttggaaaa ccgatccata cgctcgttca
     8941 acatgtcata ggcttttatt tcctcgaagc ccggcttgtg ggagcctttg accttcgaag
     9001 caaacgccga gagcattctg gtggcgccga ttttgttgca ctggggacag acggtgtctt
     9061 ccggtcgtgc gtggacggac tgggttgcct cgaactgatg ggtacattgt tcgcagcgat
     9121 attcgtagac cggcatagcc gactccttgg atggcgcctc ctggcctcgc cctgaggggc
     9181 aaggctggat aaagcgattc tcccgttgga aacgcttgac cctgtgcgta gctttgcact
     9241 attgtaccga aactttgtgc cgaaacgcga gacacaattg gaggattggt tgaaatgccc
     9301 ctgttaattc ggaagtacaa aggcagcggt ggtgtgatgc aggaagagcg tatcgacgat
     9361 ccggaccgga ttgaacgcta catgcgtctc ttcgaagaga aagacgtcaa gaagctggga
     9421 accggcgtca agaccgtgat cgagaaggac gagtggcagc tcctgcccta gcgcgacctc
     9481 ccggtttcat cccataatcg aatgttccac gccgtcacga gactcgtctc ggctcggtgc
     9541 ctgctgcgca ttgctctcct atcggggcac gtggtaggtt gagccgtgac ggtcatgacg
     9601 tattaattgg acggggtatt atggtgtatt ggcttcatat tgcacgggcg atcgatcgcg
     9661 tgacgctcca tcgggaccgg tgctccgaag tcccttccgg cgtgtcgagc agcgactact
     9721 ggcaaggggg atggttcgac taccccgata aggagcgggc gctcgccgcg atggaacagg
     9781 cgggcaccag cgaagagcgt aaatgcgcgt tgtgcaaacc ataacgccag gaatgatgaa
     9841 tcatgaatgt tgaatgatga cggcgtgatt tccggaggcg gttcctccga gcccacgtac
     9901 agcattcacc gttcgtgatt tctgtttgcc atgcctcaat tcgcgctgct tctcacgacc
     9961 tttgtgtggg gggcgacgtt tccagccacg aaggccgcgc ttgaacaaat ctccccgctc
    10021 tcgtttcttt ttctgcgatt cctcctcggc atgaccgtcg tctttgcggt gttgctgttc
    10081 ttccgccgcc ctttgattcg cgacacctcc atgatgcggg ccagtctgat tgcgaccgcc
    10141 tggctgttca tcggctatgt gttgcagacc gtgggacttc gcttcacgac ggcatcgaat
    10201 tcggccttta tcacggtgct gtatgtggtg tttgtgccgt tgtacctgct ccgtttgggc
    10261 ctccacacct gggtctccaa tgggattgca ttggccggtc tctggctgct ggtcaggccg
    10321 acggcctccg ccaaccttgg ggatctgctg actctgggga gcgctgcggc gtttgccgcc
    10381 catatggtat gcctggaacg atatacccgc gtggcggatt cggtttcgtt gttcgcgtgg
    10441 caattggtac tgatgaccgt ggccatgtcg ggagtcatgt ggtgggaaca gccgacgctc
    10501 gcgatgtttg agcccagccg cgtcctggcc gtcgggcttg tggtcaccgg tattctggcg
    10561 accggggcgt ttgcggtcca gatgtgggcg cagcggctcc tgcctgcgca gcaagtcgcc
    10621 ttgttgtttg ccgcggaacc ggccgtggcg gcctggctgg cgtggtattt cctgggcgaa
    10681 catctggacg cgcaagggtg gttcggtagt gcgatgattc tcggtggcgt cctgctcggg
    10741 tcttgggtga caggtgagtc gtcgccggcg cagcaggagt ctgtgactgc gcgttcagaa
    10801 ggagggtgag gtggctcaga aattcggcaa caatcggtgg gtgcaagagg gctttctcga
    10861 caaccgtcgg ccgggtaccg tcgttgggcg catgacgttt gcggtgttgg gcgcggtcga
    10921 tttttatctg gtcggcgatt gtcggggaga aatcgccggg aaggtgatcc ggttcaagaa
    10981 cagccgcttc gtggatgagg acctcgcggg acaggtgctg ggggacgtgg agattccgca
    11041 aatcggcgac gcgagtctta tctccttcga tccgcatccg catttggtcc cgcacccgta
    11101 tatcgagtgg ttttccatgc ggaagaatca ctaccgtatc gaattggtgc cggaggatgc
    11161 ttggattgcg acggaagaag aactggcggc gatcgaccct gtcagcctgg agattcgaga
    11221 tcgcctggcg ctgcagtacg ggcgtaaggc ggcatcggca gatgaatcgg aatgggtgta
    11281 ggtcggagtg aagggggcgg gccgtgaacg gccgcccccc ggtctcaatc aggcgagttt
    11341 attctttcct tcccgcacgt gcgcaggcac ttcccgaata tcccagatca gtcccagcgc
    11401 ttgcaggccc ctcagcatgt agtaggtgat gtcgatttcc caccaataga atccttggcg
    11461 ggttgaggcg gcatagtagt ggtggttatt gtgccagcct tcgccgaggg taatcatggc
    11521 gaggatgaag ttgttcttgc tgtcatcccc cgtcttgtag cgctgggaac catacacgtg
    11581 ggacagcgag ttgatcgtgc aggtgccgtg gagcagcgcg acggtggaca cgaagaagcc
    11641 ccaaatgagc atttgcgggc cattcgtgcc gaggctcggg gcataggctt ccaggagctt
    11701 gcctaggccg aacatcccga agccgcagat ggtcgggacg agggtatcga atcgatccag
    11761 gaagaccagt tccggaaact tggcgaaatc gccgatgctc ttcaaacgcg gcgggaaaaa
    11821 cttcttggaa ctgatccagc cgatgtgggc ccagaggaat ccgccctggc gcaccgaatg
    11881 cgtgtcttcc ggcttgtcgg agtaaatgtg gtggtgtcga tggtgtccgc cccaccaaag
    11941 tgggccgcgt tgcgcgcagg acgccccgag cagggcaaag gcgaactgac aggcccggga
    12001 ggtcttgaag gtgcggtgcg aaaaatagcg atggtaccat cccgtgatgg cgaacatgcg
    12061 tataaagtag aaccccgctg cgacccctac ggcgacccaa ctccatccaa cgatgaacac
    12121 gcccaggcag agcaggtgca cgatgatgag cgggatggcc cgcagccagt cgacggtagg
    12181 cggggcggta tccgtttgca ttttttcaat gcccgcccat gagtcaaacc agcgtatgat
    12241 ggtgatccac ggtccatcgc tcgcgggttg ttcacgtggt tggtcactgg ttttagagag
    12301 ctcaccattt tcgggtaagg tgagtggttg cagatcacta ctcatgccac ctccacagtt
    12361 gggcgaatca aggatacggt accgaggggt cgcgccgcgg ttccactagc gtgactaaaa
    12421 cgtcgcggcg cttgcgagtg atacgaatag gcaggccgct gaatgctctg aattgtgtcg
    12481 atcagtgagt gaggatactc aggctcaaag acacgtgctg ccattataca cagattcctg
    12541 gggaaatgtc ctgagaattt ttccgcatga ccgccatgtt tttttgtcga acgagcggca
    12601 gaaccggagc tgccgtcctt ccggtgtcag gatgatctca tccggcaagc cggttcggcg
    12661 caagattgtt tgactctgtt cggaccgccc ctctaaaatg cgccccatta tgaatgatcg
    12721 atttttgaaa gcgtgccggc gcgaaccggt tgattgcacg ccggtctggt tcatgcgcca
    12781 ggcgggccgt tacatggtcg aatatcgccg gttacgggaa aagcattcca ttctcgagct
    12841 gtgcaagatg ccggaattgg cggcccaggt cacgttgcag ccgatcgacc ggtttgcgct
    12901 ggatgccgcg attatttttg ccgatattct gcttccgctc gagccgatgg ggctcagctt
    12961 ggagttcgcg gagggcgagg ggccgatcat tcacaatcct gtgcgggatc gggctgcggt
    13021 agaccggctc aaggtcatcg atgacaccga actacagtac gtgatggatg ccatcagtct
    13081 gacccgcaag atgctggcgg gccgcgtgcc cctgatcggc tttgccgggg cgccctttac
    13141 gctggccagt tacgcgattg agggcggaag ttccagaaac tatatccata cgaagcagat
    13201 gatgtatcgc gagccggaaa cctggcatcg cttgatggat aaattcgcgc gcgtgattac
    13261 cggctatctt cgtcgtcaga tcaaggccgg tgctcaggct gtgcaactgt tcgatagctg
    13321 ggtcgggtgt ctgtcggccg gagactatgc cgaatacgtc atgccgcatg tgcagctgat
    13381 ttttgaggga ctcaagcacg aaggcgtccc gctgatccat ttcggcacgg gcaccacggc
    13441 cattctcaag gccatgcgtc aggcaggtgg tgacgtgatc ggtatcgatt ggcgcatccc
    13501 gatcgacgag gcctgggcca tggtgggcta cgatcgtgcc gtgcagggga acctcgatcc
    13561 ggtgacgttg ttcgggccga tctccgagat cgagcgccgc gtgacagaca ttctacgccg
    13621 ggccgcaggg cgtccgggtc acattttcaa tctgggccat gggattcttc cgaatactcc
    13681 cgtggagaat gtcgccgcca cgatcgatct ggtgcataaa ctgagtacgc ggtgagcatg
    13741 tctgtgacgc cacaaccggt tgccgtcttg ctgatggcta tgggcggccc cgattgtctg
    13801 gagaatgtgg agccgtatct tcaggacgtt cgaggcggac ggccgacttc tcctgaactg
    13861 gttgaggaaa tccgcgagcg ctatcgtgtg accggcggga aatctccggt gctcgatatc
    13921 gcgcgcgaag tggcgcgggc attggaacag cggctgaatc ggtcgggcga cgcgcggtac
    13981 cgctgttatg tggggttgcg gcactggcat ccgtttatca aggaaactta cgcggaattg
    14041 ctcgacgcct taccggaacg catcatcggg ttgtgcatgg ccccgcagta ttcttcgctc
    14101 agcatcggcg cctacatgaa aaaggtcgag gaggcgcggg ctgaactcgc cagtgagact
    14161 ccgatcagct tcgtgacgag ttggcatcgc catccactgc tgatcgccgc catcgtcgag
    14221 aatattcgtc ggacgttgga gcgattcccg gccgaggtgc gtgcacaggt gccggtcttg
    14281 tttaccgccc acagtttgcc cgagcgcgtg gtggccatga aggatccgta tcctgaggaa
    14341 gtacagggaa ccgcccaggc ggtctgcgtg caactcggga ctcagccgac gcgttttgcc
    14401 tatcaaagcc agggacgctc cggggaaaag tggcttggtc catcggttga agagacgttg
    14461 ggtgaattgg cgcaggaagg tcatcatcat gtcctggtgg cgccgatcgg cttcatctgc
    14521 gaccatgtgg aaactctgtt cgatatcgat attgagctca agcagctggc tctgacgaag
    14581 gggctccagc tggaacgtat tcccatgttg aacgcctcgg ctccattgat cgnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattagcgg caccaccagt ggcgcgcact
    14761 ccacggtcag ttgtcattat cggcggaggt atctcaggcc tctccacggc gtttgctctc
    14821 caggagcagg cggccgcggc tgatatgccc ctcacctgca ccatcctcga tgcggcgccg
    14881 gtctggggcg gaaaaattct cacgcatcgg gtcgggcagt tggtcatgga agcggggccc
    14941 gactcgttcc tctcgcaaaa gccctggggc atggagctgt gccgacgtct tgggctagcc
    15001 gatcaactga tcaacaccaa cccggttgag aagaaagcga gtgtcttaag aggcgggcag
    15061 ttgcacgagt tgccggaagg gctcgtgaca ttcacgccga ctcagctggg gccgtttttt
    15121 cgcagcgggt tgttatcctg ggtcgatctt gcccgtatgg gctgcgatgt gctgattccg
    15181 ccgcgtcggt ccaccgacga cgaatcgctg gcgtcgtttt tccgtcgccg gttcggacgg
    15241 catgcctgcg aacgggtcat ggagccgctg atggccggca tttatgcggg cgatgcggag
    15301 cagatgagcc ttcgggcgac gttcccgcgt ttctacgagt tggagcaggc gcatggcagt
    15361 gtgattcgcg gcatgatggc ggcccgtcgt gcacgggcgc agaaggtttc cggcggaggc
    15421 ccccgacaca cgatgtttgt gacgctgaaa aacggcctgg ccgatttggt ggctgggctg
    15481 acggcgcaga tccagcaggc cggcggggtg ctgaaagccg gcgttcaagc ggaggcgttg
    15541 cgggtgcgtt cgcaccaggc cggtcgctgg atgtacgatg tgatttgcac cgacgggacg
    15601 gcgctttcgg cagaagccct ggtcttggcg acaccggcgt atgtcagcgc tgaactcgtt
    15661 cggccgttaa cgccgatggc ggccggcttg atggacatga ttccctatgc ctcgacagcc
    15721 acgatctctc tcatctatcc tgccgaagca gtggggaacc ggctccaagg cttcggcttc
    15781 gtggttccac gaatcgaggg ccgtgatctg attgccgcga cctggacctc cctcaagtgg
    15841 ccccatcgag caccgccgga ggatgtgtcg gtgcgttgtt atctcggcgg ggtcgggcga
    15901 gaagccatct tgcagcggga cgatgaggcc ttggtccagt gtgtgcgcga ggaattggcc
    15961 tctattgtgg ggctgctggc gacaccgcac tatgtcgagg tgaatcggtg gaatcgggcc
    16021 atgcctcaat ataccctcgg acacctcgat cggctcgcgc agctggaggc ggcgctctcg
    16081 cgattcggtg gcctggttgt caccggcgca ggctatcgcg gtgtgggcct tcccgattgt
    16141 attcgagacg gcgctgagac ggcggcgaag ctgctccggt atttacaggc ggcgccggtc
    16201 taaggtttca atgacgatgg gagcgggaag agaaggaata cccagatgct gaacgaccaa
    16261 ttggtgatct ccgatgtgac cggtcccttt cgcgagccgc gggagccggt gttgtcctac
    16321 gactattcga ttcaacgggc gacctgggcg gcaacccatg ccgttcgagt gaagattgcc
    16381 caggcggagg agttggacta tgtgaaggag aagttgctgg gcactgtcac tggcagcccc
    16441 ggccagcagt tgatgctgaa caaatttctg tcccgcaaga tcggtgacca gaaggttcgt
    16501 attgccgagg ccgaagggtg gttgaaagaa cgcggtgatg tgttggtggc ccccttcacc
    16561 ggttcgctcg ctcattactt cccgcaactt gaggcctggg tccaagcgga gcaggataca
    16621 ttgcgggcgg agatcaagaa cctggtcggc ctggtggcca gtcctccggc cgtctgacga
    16681 ttgcgccctc tgtcaccttc ggtccactgc tcgtccagat ttcatccgtt actccgaagc
    16741 cagcctcctg tgacgtgccg caagccattt ctgcgcccag ccgatgagcg ttccggtact
    16801 tgtcagttga cggttctgtc gccatttccc caacgcacag aatctgtgga taagcgtgtg
    16861 aatatcagcc gcctcctcgc ccaagcccct cagtagagcg gcccttgagc ggcctgcaca
    16921 agttttaggc attggggtgg tctctatccg gaccactaga tgctgtatgg gatgaaaacc
    16981 cttaggaaga ttatgcttgc tgaatgcgag cctggggata gcgaaggcag tctgggcctg
    17041 gagtttcgcg gcagcggctc aaaaaatacg ccgatattgt ttgctatgga ctccggaatc
    17101 gtcggcaaat cccagcgatc cggtgtcggg attgctggtg tcgttcaggt cgatgcgata
    17161 gtgcccaagg acgtgttcca tcgcttcttc gaaggggatg gcctgcgggt agaggttgcc
    17221 cggggcgtgg gcgccgcgag ccagggtgcc gaccgcttcc ggagttgcat atttggggtc
    17281 tgaaaaaatg tacagatcgg ccacggcctg gtccccgagt ttgtgccctg ggctggtggc
    17341 cggcaacatg gaggggagac tctgagccag ccgggcctgc ttgaattgtt tgaggaggcc
    17401 caccacctga ctggtcgtcg cctgcgccgg caccacgata ctgacggcat taaactccgt
    17461 caacgtcgac tggactttat acatgaccgg ggccgcatcc ggatcttcgg tcgtcacctg
    17521 cgaaatctcc ggaccggcgg cctgcttttg tttgccgctg ggaacggcca ggttgatcag
    17581 cagccacatc accagaatgg ccccgaacaa gacgatgagc ggatgaagcc cggctcgctt
    17641 gccttcttcg tatggatcct gctctgtgct catggatgag tcctggtcgg ctccgtcgaa
    17701 gggacgaggg gagggacggt gcccgcgggt gctgtcgatt ccaccaactt cgcggcttcc
    17761 cagtagtgat ccatttccac cgtggagagg tcgttgacgc tccgtccgct tcgagccgcc
    17821 tcgcgctcaa tgaactggaa tcgtgcggtg aatcggttcg tggcgagacg gagcgactct
    17881 tccggattca ccttgatgtg ccgggcgacg ttgaccaggg aaaacagcag gtcgcccatc
    17941 tccgcggcca tttccggggt cggctccggc ttgggttcgg ccacggagcg acgcgtagcc
    18001 gcgtcggtga gagcgtgctt gagttccccg atttcttcct cgactttagc cagcacggcc
    18061 gccaggccct gcgggctgtc cggccagtcg aacccaaccc gcgccgcgcg gacctgcgtc
    18121 tgataggcgc ggagcagggc cggaagggcc tggggaatgt catgcaggac cgaatccggt
    18181 ttgccggcgt tcttgcgttc ggcccgtttg atatcttccc accgatggac gacctggtcg
    18241 gaattaagtg aggactggct ggtcgccccg tcaccgaaca cgtgaggatg gcggcgtacg
    18301 agcttgtcgc tcaactgttc caagacgtcg tcgatggtga acgtgcctgc ttcagtgccg
    18361 atctggctgt gaaacaacac ctgcagcagc acgtcgccca attcttcctt tagcttggcg
    18421 aaatcctgcc ggtcgatcgt atcgagggct tcataggttt cttcgatcaa atagggcttg
    18481 agggattcgt gggtctgctt gcgatcccag gggcatccgc ccggtgcccg aagccgggcc
    18541 atgatggcca cgacgttatc gaaacgtgct gacatgcgaa cccctccgtc gcgccactct
    18601 aactgagttc ttcagcacct tcaacggggc ctctcgtctt gcgcctcttc aagccctgtg
    18661 gtagggtacg gccctgcatt ttccattggt tgatcggagg cttttttatg ggtgatgaca
    18721 aggaccaagg attcgtcatc cgcgatcgtc gaggacgggg tgaagatgcc cccccaccgg
    18781 cccaggcggc gacgtctccc gcagcagagc cctctgccgc cgcgcctcac cacgaccatg
    18841 atgccgattc gcatacgcac tcagggcatt tgccggtcaa cttttcctcc tttgtgatct
    18901 ccatggggag ttcagctctg atgctgatgg gggagcaact cgatcctcag cagccatcca
    18961 tgccactgaa ccttcctcaa gccaaagaaa tcatcgatct actgtccatg ctggaagaaa
    19021 aaacgaaggg caacctcacc ccggacgagc aagtcgtaat gaaggacatg ctctacgcct
    19081 tgaggatgaa attcgtcagt ttgacctccg ggaaatcgac gcttcatacc ccgtaacctc
    19141 gtgagttgct tcagcgcgcc gtcctgcttt gcggtgtcgg tccttccttc gggtgacact
    19201 gtgttcttca atgccctgct tccggtcgtt tctcgatgaa gcaagggttg ggttgcgcgc
    19261 gccctctttt gagactccac ctggaattgg ttatagtgag gcaggatcga atgggccggg
    19321 cgtgagtcgg agtatcgaga atgcctgaat cgacggatat gacgaagctc atccccccgg
    19381 gggtgctgcg cgagaaaaag ccgtcgacgc ctgcttccat tgagagcgag cggcggaagc
    19441 gtcatgtccg gccagtgtgg atcgtgctgg tcctgctcct gccctgcctc gccctcactt
    19501 tttactacgc tcaaatggcg gtgccggtcg gggaggagtc ggactccttt cttcccagca
    19561 ccgggtatgc cttcgtgctg ctgctggtca atctcgattt gatcggcttt gtcgtcctta
    19621 cgctactcct ctcgcgcaat ctcatcaaag cctatttcga acggcgacat cggctcgttg
    19681 ggtcggggtt ccgcgcgaag ttggtcgccg cctttatcgg cttttccctg attcccaccg
    19741 tgctcctggc cctggtggcc agcggcctgg tcaacaaggc ggtcgatgtc tggttcaacg
    19801 accagatcga gcacgtgatg aaggattctt acgaggtcgc gcgcatgcac catgccggtc
    19861 atgtgtccct ggcgatcaac agcgcacgcg ccatcagcca cgagattttt cgcgaggagt
    19921 tgctcttgcc cgagcagcgg gatttgctgg ttgcggcgat cgcccggaag cgcgcggaat
    19981 atgccacggc cgggatcgag gtgttttcct cgaagatgga aacgttgacc aagtcgctgg
    20041 atccagaggt gccggtggcg gtgctcgatc tgccgatcgg ccaactggtg ctacaggtga
    20101 tcaacggcaa gcaggaactc acgtccgtcc aggaagcaca gacgggacgg ctcgtgaggg
    20161 ccggtgtacc gatcgcctcc agcatccgcc gtggagaaat cgacggggtg gtggtggtcg
    20221 atgcctatgt gccggaatcg ttgctcggca agatggagag catcgggcgt caatataccg
    20281 aatacaaaca gatgaaggcg atgaagaatc ccatcaaagc gggagcgtat ctgctggtgg
    20341 cggtcatcac ggtcatgatt ctgttcagtg cgacctggtt cgggttttat gtggcgcgcg
    20401 gcattaccgt gccgattcaa cggttggccg aggcgacgga ggcgatcgct caaggggatc
    20461 tgtccgtgcg catcgaagcc aaggcgacgg atgagatcgg caccctggtc gaatccttca
    20521 atcgcatgac cgccgatctg cagagcagca aaacgaaagt ggaggaagcg aacgtctcgc
    20581 ttcggcaatc caacctcgag ctggatcgtc ggcgcgctta cattgaaaca gtggtggata
    20641 cgatcgccgc gggactgctc tccatcgacc ggcaggggat cattaccacc ttcaatccct
    20701 ctgcggagcg tatgctggga ttgtgggccg atcggtttcg gggccggtcc gccaacgagg
    20761 tgttcaagga gtacaagctg gatttgtttc agtccgtcta cgatcggatg ctggtcgatc
    20821 agcgagacaa tattgcgctc gaaggacaac tggatttgca gggtcggttt ctgaccatcg
    20881 gcgtgcattg ctcccgcatg aaggatgagt cgaacaagga cctcgggttc gtgctgattt
    20941 tcgaagacct ctcggaattg atcaaggcgc agaaggcggc ggcctggcgg gaggtcgcgc
    21001 agcggatcgc tcatgagatc aaaaatccgc tgacgcccat tcagctctcg gcgcaacggc
    21061 tgcgaaaaaa gtttcaggat aaggctccgg atttcgaccg gatttgcgat gagtccacgc
    21121 aagtgatcat taatgaggtc ggcagcctca agcaaatgct ggacgagttc tcgaagttcg
    21181 cgcggatgcc ggctccgcac atgaccatga actcgctgga tgatgtagtg aaggaagtgg
    21241 tggcgctgta tgacagcgcg catcgcgaag tggaatgcgt ggtgacgctc gatgcggatc
    21301 tgcctccgtt caatttcgac cgggaacaga tcaagcgtgt cctggtcaat ctgtgcgata
    21361 acgggatcca cgcgatgaac cacaagggac atctgtggat cacgacccgg tacgacacga
    21421 aacagcgtcg cgcagtcgtc acggtggcgg atgaagggac gggtattgcg ccggaggatc
    21481 aagagaaatt gttcgtgccc tatttttcac ggaagaaaac gggtaccggg ctggggctgg
    21541 cgatcgtgcg gcggattgtg accgatcacg acggccagat tcacgccgga aaccatcagc
    21601 ccaaaggggc cttgttcacg ttcgagttgc cggtgtgaac ggttcccgcg acgcgtagtg
    21661 tggagaagac atgtctgctt cgattctgat tgtcgatgat gaagtctcca tcctgaactc
    21721 cttgagcagc atcttagagg atgagggata tgaggtcagc gtggccaaga gcggcatcga
    21781 ggcgctcaag ttatgtgccg tgagtcctcc cgaactgatg atgctggata tctggatgcc
    21841 cgacatggat gggttggaga cgctccggcg cttgcgtgag ctggtgcctc agacgcaggt
    21901 catgatgatg tccgggcatg gatcgatcga gacggcggtg aaggccatca agctcggggc
    21961 ctacgattac attgagaaac cgctctcgct cgaaaacgtg acgctacggg tcaaacatgc
    22021 cttggatcag caccggcttg agcaggagaa tcggacgctg cgcacgaagg tagagcggaa
    22081 gttcgagttg atcgggcagt cgctggcgat gcggcagctc aagcagatta ttgaaacggc
    22141 ggggcctacg aacagccggg tcttgatcgg cggcgagaac gggaccggca aagaactcgt
    22201 cgccagggcc atccatcaac agagccccag ggcgaaccgt ccgtttgtgg cggtgaactg
    22261 cgccgccatc cctgaaacct tgatcgagag cgaattgttc gggcacgagc gcgggtcctt
    22321 cagcggcgcc acaacgatga agcggggcca gttcgaacag gccgacggcg ggaccctgtt
    22381 cctggatgaa attgcggata tgagtctgag cacccaggcc aaggtcttgc gcgcccttca
    22441 agagcagcaa tttacgcggg tcggcgggac caaactgatc aaggtcgatg tgcgggtcct
    22501 ggcggcctcc aacaaggatt tgctgaagga aatcgagaag ggcacgttcc gggaggacct
    22561 gttttaccgg ctcaacgtgg taccgatcgt ggtgccgacg ttgcgggatc gccgtgagga
    22621 tatcccgttg ctggtgaaac atttcctcca tgtgcatgcc gaggagcaag gcttgaaaat
    22681 caagcaagtc tcgccggagg ccatggacgt gttcatccag tatgaatggc cgggcaacat
    22741 tcgcgaactg cgcaacctga tcgagcggct catgatcatg gtgccggggc ctgtgatcga
    22801 agcttcgcat gcgtcggtag cgttgcaggt gcgtcctcag ctgtccgcta cgcaacaggc
    22861 cgccggggtg caggcgccca attccctctt gacgaggcat tacgactcgc tccgcgatgc
    22921 ccgcaatgcg ttcgaaaagg aatttatcgc ccgcaagctg cgtgaacatc attggaacat
    22981 ctcccgtacg gccgaagacc tgcagatcga gcgcagccac ttgcatcgca agattaagtt
    23041 gctcgatgtc gaaatgcgcc ccgagatgta gccgggctgc tgaaacaggt ccccaacatc
    23101 gttctcggct cgaatcgatc ctcaacgtac ccccgagggt acgcctccgg ttccttctcg
    23161 cccgcggcct tgttggatga cctgtttgag cagaccctgt agtttgaccg ttgagagtca
    23221 cacgaagggt gtgaacgcgt gagactaatt gttgacccgc tatcagtcag ctcgttaaga
    23281 tgcggcccca atgactacct atcgtgatgc cggagtcgat atcgatgccg gcgatgaatt
    23341 cgtcgagcgg atcaagccgc atgtgcgggc gacctttcgc ccggaagtga tgaccgatct
    23401 gggcggcttc ggaggcctgt tccgattcca ggcgaatcgc taccaggatc cggtgctcgt
    23461 gtcggggacc gacggcgtcg gaaccaaact caaaatcgcg ttcctcatgg acaaacacga
    23521 cacggtcggg atcgacctgg tggcgatgtg tgtcaacgat attgccgtca gcggcgcaga
    23581 gccgctcttc ttcctcgatt actttgcgac cgggaaactc gccttaaaga cggccgaggc
    23641 cgtggtccgc gggatttccg acgggtgtcg tcaggccggt tgcgctctca tcggtggaga
    23701 aaccgccgag atgccctcgt tctatgcgga gggcgagtac gacctggccg gttttgctgt
    23761 gggcgtggtc gatcgcccga agatgatcga cggccggcag attgttcccg gggatgccgt
    23821 gatcgggttg gcctcgaccg gtgtccacag caacggcttt tcgctggtgc gcaaggtgtt
    23881 gtttgagaaa agccgactga cggtcgagag tgtgatgccg gaactcggtg gtgcgctggg
    23941 tgagaccctg ctgacgccga cgcggattta tgccaaacag gtgctttcgt tggccgaggc
    24001 ttgcccgatc aaggggatcg cgcacatcac cggcggtgga atcaccgaga atctgccacg
    24061 ggtctttccg gcccgctgcg gggcgcgcat tcgtcgcgga tcctggccgg tgctgccgat
    24121 ttttcagacg attcaggacc gcgggggagt ggagctgagc gagatgtacc gcgtcttcaa
    24181 tatgggcatc ggtttgattc tggtcgtggc gccggaacac gtcgatcgcg tgatggccaa
    24241 agcggtagaa gcgggcgacc gggcctatca catcggcgaa atggtggcgc agtccacaga
    24301 cgaaggtgtg gtcgagtatg tcgggtaaat caccgcgcct ggtgcgcctc ggcgtattgg
    24361 tttccggccg aggttcgaat cttcaagcga tcatcgacgc gatcgaggcg gggacgttgt
    24421 cggccgaggt tgcggtcgtg ctcagtaata agcaggatgc cggcgggctg gagcgggcgc
    24481 gaaagcatgg cgcgcccgcc gtgtggctgg atccgaagcc gtatgcaggc cgtccggaca
    24541 gtcgcgaggc tttcgataag gcggtccttg aggtgctgca gaagcacgaa gtggatctcg
    24601 tgctcctggc cgggtacatg aaaatcgtca cggcggtgct ggtcaaggcc tacgaaaacc
    24661 ggatgatgaa cattcatccg tccctgcttc cgtcgttccc tggtctggat gtgcagaaaa
    24721 aagccatcga ccacgggtgt aaaatcgccg gttgcaccgt gcactttgtg accgaagggg
    24781 tcgacgaagg tccgatcatc atccaggcgg ccgtgccgat tcgggaggga gatacgccgg
    24841 atactctggc tgctcgcatc ctggagcagg aacaccggat ctatcctcgc gcgattcaac
    24901 tctacgcgga gggacggctg cgggtggagg gacggcgggt atcggtggcg gacgcaggac
    24961 cggtggcgac gggatttcac aaccccggat aaagtcttcc gccgatctgt aagttcgtat
    25021 ggagacgcgc gctccggttg tgtataatgc cgcgtgagag ttgtgggggg ctagctcagt
    25081 tgggagagca ctacgttcgc aacgtagggg tcgggggttc aactcccctg ccctccacca
    25141 aacctcactt cctcttaccg gtgcctcatc ggcgctagcc ttcttccttg cggcgccgga
    25201 tcatatctcc cgtggtttca ttttcttacg catccgcgcc ggcgcgtctc atgaggccac
    25261 gccgctcttc cgagtctgga ggagcgctgg tcattggccg gtgccctcga actggggcat
    25321 ccattttcgt gtccatcacg tgcctaaccc ggagcggatg cagtcgctcc acactgagga
    25381 cggaggctca ggacggatct acgattgcaa gacctaactc tgttcagttc cttcaattca
    25441 cggtcacaaa gtctatggcg gtcagcgctg gcaattttgt cagggtgttc ggagggaatg
    25501 ctccacaatg gggcgaactc gggaatgata gacttcggca acagttaaga aagcagtgaa
    25561 cctagaattc tgtagggtaa gtatagagaa gacggaaatg gtatgcgaat tacgggagca
    25621 ctgtgtttta tggcattaaa ttggccactc agaagagtac agaacctgag gctaggaatc
    25681 ttgatcatcc tacttcacct tacggtgctt gtaggttgct ttcccaaggc aacaggccca
    25741 ctctttcagc gttcaatag
//
LOCUS       DAOQRM010000017        25548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1213_length_25548_cov_15.979328, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000017 DAOQRM010000000
VERSION     DAOQRM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25548
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1213_length_25548_cov_15.979328"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="PKK81_03010"
     CDS             <1..1427
                     /locus_tag="PKK81_03010"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNV31869.1"
                     /translation="PVRLHRGSMLKRHSQFLKSLLFCIDLGLICACWVGAYYLRFSEI
                     VEPATKGIPPLRIYLLLLVPIIAVWGMSFQAFDLYRPRRMGTRLSEFLDVAKANTLSV
                     LILVALTFFFRQYEYSRLVLLYFWLLNLITLGFSRVLFREALRFLRRHGYNQRHALVI
                     GTSRLGRRVVDALAQHPELGVKVHGFLSADSRKVGERINGVPVIGRYDDLQERVQSGV
                     DIVFVCLPPEDEQWAEKMFTCLATTMVEVKALPAMCEFVSLRAEAEMFEGLPLITLQG
                     SPLYGWNLVIKRVLDVVGASAALVVFSPVLCAIAVLVKLTSPGPVFFRQLRMGLDGQA
                     FEMLKFRSMKLNAESETGPVWTQPNDDRRTPIGAFLRRTSLDELPQFWNVLRGEMSIV
                     GPRPERPEFIARFRETLPQYMLRHKMKAGITGWAQINGWRGNTSLERRIEHDLYYIEH
                     WSIWFDFKIMLLTVWRGFIHRHAY"
     gene            1565..2104
                     /locus_tag="PKK81_03015"
     CDS             1565..2104
                     /locus_tag="PKK81_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNV31870.1"
                     /translation="MPSTTRRTKTSAKPRFSLQETLRGIRLFATDVDGVLTDAGMYYS
                     ESGDEWKKFNTHDGMGIKLLQKAGILTAIITQESTKIVMRRAQKLTIPEVHQGVYDKL
                     AVLKELIARHGFSMDQVAYIGDDVNDLQALGAVGFSAAPADGMPPVLKTVRYVCKKKG
                     GEGAVREVADLILAARQPR"
     gene            2146..3597
                     /locus_tag="PKK81_03020"
     CDS             2146..3597
                     /locus_tag="PKK81_03020"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNV31871.1"
                     /translation="MDMTEHLYPVILAGGSGTRFWPLSRHLYPKQLLRIIGDETLIQQ
                     TMRRVLSCAKPEQVIISTNPGQADSIRVQLGEWKSALKNNYVVEPVGRNTAPAIALVA
                     AELLLRDPHAMMLVLPADHVVTGEKAFKAAVALGVQLAEQGHLVTFGIKPTRPETGYG
                     YIQPNRRKSLAKKGRLTGHPVARFVEKPNRTKAAQYLKNGHFYWNSGMFLWKAATILE
                     EIQQHQPQLAKAIQRVHALMASGAEPGLVEAAYKKVPSVSIDNGVMEQSANAAMIPVG
                     FGWSDVGNWSSLEEVAPRDKAGNVVSGRVIDMDSTNSVLYADRRVVATIGLSDMVVVD
                     TPDATLICPKSRSQDVKQMVEILKQQGAPEHLEHLTVFRPWGSYTVLEEGPGYKVKRV
                     TVNPGGRLSLQLHHKRSEHWVVIAGTARVTRGEELLDLRVGQSTAIPVETPHRLENLG
                     SETLHIIEVQNGPYLGEDDIVRFQDAYGRTSVR"
     gene            3679..5079
                     /locus_tag="PKK81_03025"
     CDS             3679..5079
                     /locus_tag="PKK81_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNV31872.1"
                     /translation="MALFREYDLRGVVGEELTEAIAEQVGRAYATMAREQGASRISLG
                     RDGRLSSPGLQQALLRGLLAGGLDVVDLGLCASPLLYFSLFTLPVDGGIMITGSHNAA
                     EYNGFKICIGKEAIHGEEIQRLRRVMEAGQFTTGSGRLSSHPIIPDYLEHLKTNFSGV
                     RADHLHVVIDCGNGAASLVAKQALEQMGCRVTGLYDELDGRFPNHHPDPTVVENLQDL
                     IATVREKKADVGIGYDGDADRIGAIDERGEILWGDRLMIVYARDILTQRPGTTFISEV
                     KASQCLYDDIAAKGGRPIMWKTGHSLMKAKLKAESAVLAGEMSGHMFFADRYFGFDDA
                     IYASCRLVEILAKTKRSLSSLVADLPQTTVTPEIRVDCPDTLKFQLVDQVRTQLAAYL
                     AEGQPVGASNLRMRELVTIDGVRAIFEDGWGLIRASNTQPALVLRFEAPSPARLDVIR
                     ATIETELAQARRVLST"
     gene            5081..5893
                     /locus_tag="PKK81_03030"
     CDS             5081..5893
                     /locus_tag="PKK81_03030"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNV31873.1"
                     /translation="MSRYSASLRLSLFLAVTILATVVASAPLLSYATPDGQILTPAAL
                     PFANGERLSYDVTWLGMRAGIATMIVQEGPVEGPRPQLTFAMTARSSPTVTKFYPVDN
                     RGVSVVDRESFLPRHMTFARREGKRFNDFDYTFRHTEGLVTAVKDGKSDELPIPSDAQ
                     DAMSCLYYVRKVLPFVPGASLAMTVHHDKKNYKMDVRVEALETVEGAWGKRQTARVVV
                     IMPFQGIFLNEGNIRVWFTNDEHRVPVRMKAKVVVGSIVAELTDGYRPTSQP"
     gene            6027..7025
                     /gene="fbp"
                     /locus_tag="PKK81_03035"
     CDS             6027..7025
                     /gene="fbp"
                     /locus_tag="PKK81_03035"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNV31874.1"
                     /translation="MAKFPITLSRFIIEQQAAHPEATGEFSVLLTQIGLVGKMIAHDL
                     RRAGLNKILGTTGETNIQGEVVKKLDQIANDTFVRVFEHSGLVCVLASEEMEKPLKMV
                     HEGAKYMLLVDPLDGSSNTDVNMPLGAIFSIRRSQLGQRQSVEDELLQRGTEQIAAGY
                     VLYGASTMLVFTVGQGVHGFTLEPSIGEYLLSNENIRIPSRGKVYSVNEGNYHRWPAG
                     TQKYLDSLKVQDKATGRPYSGRYSGCLVADVHRILLGGGIYLYPGETAKPEGKLRLMY
                     EGNPLAMVVEQAGGKATTGTMRILDVEPKALHQRVPLIIGSAEDVAQAEAYIQGRA"
     gene            7062..7985
                     /locus_tag="PKK81_03040"
     CDS             7062..7985
                     /locus_tag="PKK81_03040"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNV31875.1"
                     /translation="MSNRVQEILSWYESDNAGTKTNIARLLNHGKLAGTGKLVILPVD
                     QGFEHGPARSFAPNAGGYNPHYHFQLGLDAGCNAYAAPLGFIEAGAGHFAGQIPLILK
                     LNSHDTLHDEKDPMPSVTGSVRDALRLGCSAVGFTIYPGSSHCNAMYEQLRAIAEEAK
                     SCGLAVVVWSYPRGSGLSKEGETALDVVAYAAQIAAQLGAHIIKVKLPTAHLEQAAAK
                     KVYEAEKVPISTLAERVKHIVQSSFDGRRIVIFSGGAKSDEKTVFEEVRAIRDGGGFG
                     SIIGRNSFQRPKAEAVKFLNTIMGLYAGEKP"
     gene            8013..9557
                     /locus_tag="PKK81_03045"
     CDS             8013..9557
                     /locus_tag="PKK81_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNV31876.1"
                     /translation="MNDFQIPTEQQPPRRSWIFPALLLIAGVLIGVILTSDLGWLPTG
                     HAVPEPVPPPVATPVSTAVQPTLPGAGGQSFVDVAKVVKPAVVNIFATRNGSSEEGKG
                     TPFEDPFFRRFFGEEWMKRFEAPKERKERGLGSGVIVDPNGLIITNNHVVSKADEIKV
                     FLSDKREFKAKLVGTDAKTDVAVLKIEAGGLPTVAWADSDKLEVGEFVLAVGNPFGLT
                     QTVTLGIVSALGRAAGIAEYEDFIQTDAAINPGNSGGALVNVRGELVGINTAIYSQSG
                     GNMGIGFAVPSNMAHSIMEQLVQHGKVVRGWLGVSIQELTPELSSQFGVPKDVKGVLV
                     SDIMDDSPAKKAGFERGDVIVEYDGKPMDSPAHLRNAVAQTVVGKKVTVKLIREKKPK
                     SIELTIAEQPKNLAQAGVEEGGESVAPAGLLSDVEVRELNTELAGRYGLKSSDHGVVV
                     VRVKPGSPAEEAGVREGDLVLEVNRKAVGTLKAYEHVAANLPKDQAVLLLLRRQGRAI
                     YLTLRP"
     gene            9608..10633
                     /locus_tag="PKK81_03050"
     CDS             9608..10633
                     /locus_tag="PKK81_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNV31877.1"
                     /translation="MVTRAIFVLLSALAGMALFLRAQDPSREFLLMGLGMGGVAGGLI
                     LAGEYALRRLSFGIIVGGAVGLAGGLILTGLVEWVGSAVFDVETFLFHIGGLVFLLGL
                     PYLGLAMGARFGNEQFPGLDQGPTAAGASHASLKVLDTSVIIDGRVADLCETGFLEGP
                     FLVPHFILNELQHIADSSDSLKRARGRRGLDILNKIQKMPGIDVRIIDEDFPHVKEVD
                     AKLVVLAKKVGGRIVTNDLNLNKVAELQGVRVLNINELCNALRPVVLPGETIRVFVLK
                     EGKEAGQGVAYLDDGTMIVVDNARRCIGRNVDVTVTSVLQTTAGRMIFTRLKEESERE
                     EYQVARG"
     gene            10753..11496
                     /gene="ispD"
                     /locus_tag="PKK81_03055"
     CDS             10753..11496
                     /gene="ispD"
                     /locus_tag="PKK81_03055"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNV31878.1"
                     /translation="MSARNAKPSLAGPRTVALVPAAGRGLRMGGRIPKQFLALGGRPI
                     LAQSLLVLQASPLIHEIILAVPESERQYCLDHIVATGEFSKVTKVVPGGAQRQDSVRH
                     ALAEVSQETEMVLVHDAVRPFLTEDMIRRVVAAAVEHGAAIIALPMRDTVKYVGAGGV
                     IERTVDRRPLWLAQTPQAFRRDWLEEGHHKAFLAGVQATDDAHLVELIGKPVVVVEGS
                     GENIKVTRPEDLVIGEAILSSRTAARSAE"
     gene            11493..11975
                     /gene="ispF"
                     /locus_tag="PKK81_03060"
     CDS             11493..11975
                     /gene="ispF"
                     /locus_tag="PKK81_03060"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNV31879.1"
                     /translation="MTAVRVGCGWDIHPMVAGRKLILGGLEIPHHKGLQGHSDSDALV
                     HAICDALLGAMGEGDLGRHYPSSDQRFKNISSLKLLEDVVQKLRAKGYRLANVDSTII
                     AQAPRLSPHLAAMQKIIAGVLEVDPDLVNVKVKSGEGLDAVGREEAIAAQAVCLIERG
                     "
     gene            12015..12665
                     /gene="cysE"
                     /locus_tag="PKK81_03065"
     CDS             12015..12665
                     /gene="cysE"
                     /locus_tag="PKK81_03065"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNV31880.1"
                     /translation="MFKTISQDLQAIFDRDPAATSRLEVIATYAGFHALLAYRLAHWL
                     KGHRVPFLPRAISQLARWLTGIEIHPSAKIGERFFIDHGMGVVIGETAEVGDNVTLFQ
                     GVTLGGTGKERGKRHPTLGSHVVVGAGAKILGGITIGDNVKIGANSVVLKSVPPNSTV
                     IGVPARIIKSQGERLPDATMDHTNMPDPTADRFAALELELIELRKKLENRDPNSPR"
     gene            complement(12811..13128)
                     /locus_tag="PKK81_03070"
     CDS             complement(12811..13128)
                     /locus_tag="PKK81_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HNV31881.1"
                     /translation="MKLLCTTLSVLMLAAVLATPQLSYAGAQQNKMKTCNADANAKGL
                     TGEGKGDARKAFMKECLSAKPEKAAAGASQQNKMTTCNKDAKAKKLAGDERKKFMKEC
                     LSN"
     gene            complement(13210..13971)
                     /gene="truA"
                     /locus_tag="PKK81_03075"
     CDS             complement(13210..13971)
                     /gene="truA"
                     /locus_tag="PKK81_03075"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNV31882.1"
                     /translation="MTTFKLVLEYDGTQYAGWQRQLNVPTIQATVEDALAAIAQTRLT
                     LVGAGRTDAGVHAFGQVASFRTDRRLSQREWLRALNAHLPADISAISVEAVPDHFHAR
                     YSAKGKLYEYHLMNRPERAPLLRERAWMLYKPLDFAAMTEAAACLTGSHDFSSFETAP
                     TDNENPQCRLQQADLRRQGDLIILSFYADRFLKQMVRAMVGTLVEVGQGKRTAPDMRT
                     VLAARARAAAGRTAPAHGLYLVRVDYTEGSIPSLP"
     assembly_gap    14069..14168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14174..15502)
                     /locus_tag="PKK81_03080"
     CDS             complement(14174..15502)
                     /locus_tag="PKK81_03080"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV31883.1"
                     /translation="MSHVASLIRHLLTFAFACVLFGTGISSGGEIQTEPSWPTVPQVA
                     AEEPSPRFLLVKQERAGASRYPIIVRDLRTWSTADGTRLVLDLNHKTSFSETHLRNPD
                     RVLIEVNNAILGKSSRQRVSGGTIPQSFQIAQSRPRVVSITLTRTQNSRYKVFSLDNP
                     DRLVIDITQRPKDQIRQIGETSATSPQAPAVPLPTPPAIPSPTVTEPARPVAPKPASP
                     VQTIVIDPGHGGKDPGTIGQHGTTEKEVTLKVGLLLKSLLSTVPNTRVLMTRERDAFI
                     ELEDRAKFANGKDADLFISIHVNSHPHKGVRGLEIYHFGEAKDQRALEVAARENGTPL
                     NNTGVGWEYIVADLLTSKKIEHSQELAWTAKQAMVTNLNGRYSTIDHGVKTAPFYVLR
                     FTTMPSILAEIAFMSNPAEEEQMRSQPFLAHIAESIFEGVKTYLHTNPPR"
     gene            15596..16129
                     /locus_tag="PKK81_03085"
     CDS             15596..16129
                     /locus_tag="PKK81_03085"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNV31884.1"
                     /translation="MATHLLVDGYNLVGSAGMALPAGSGRLEAARETLLKNLAGYRQR
                     KGHAITVVFDGWQGGLGAEHREFQSGVEVVYSKRGERADQVIQRLARLYGRACAVVSS
                     DHEVVNSARAAGAFVIGAAEFRAKLQDRSSSLSTVAFKELDRGDLDRVKRSKDKGGNP
                     RKLPKSQRKRNHQLREF"
     gene            complement(16116..16895)
                     /locus_tag="PKK81_03090"
     CDS             complement(16116..16895)
                     /locus_tag="PKK81_03090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNV31885.1"
                     /translation="MLIDTHTHLDDARYESDREAMIARARAAGVESMITIGCDLATSR
                     SAVTLANQYPFVYASIGVHPHEVKHIEDGWYDEFRRLARDKKVVAYGEIGLDYHYNHS
                     DPELQRTRFREQIQLARELKLPVIIHTREAQDDTIRILKEERASEIGGVFHCFSGDAW
                     LAKDAIELGFYLSFSGILTFQNATMLREIAKTVPADRLLIETDCPYLTPVPHRGKRNE
                     PAFVRHVAELLATITADNVPSTAEDVARRTSENARRLFKIP"
     assembly_gap    17245..17344
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17345..>21982)
                     /locus_tag="PKK81_03095"
     CDS             complement(<17345..>21982)
                     /locus_tag="PKK81_03095"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNV31886.1"
                     /translation="PKVAHRTSPTNIGLALLANLSAYDFGYLSAGQLIERTAHTFDSM
                     ATLERFRGHFYNWYDTQTLKPLLPMYISSVDSGNLAGHVMTLHSGLLSLPEDKILAER
                     TFEGLRDTLALLSEALETPTSQVDALQKNLAAACDNRPTTLRQIHHTLTLLTKQVDEV
                     TAHLDPASNAEAHRWAHAFARQCRDTVAELMILAPWIGLAATDEILRLFPELDQIPTL
                     RTLTRLEGEWLPAIDARLGPDASGTERTWLIELRRHLSAASRLAEQRLASLDHLARQA
                     NQFAQMEYDFLFDDTRFLLSIGYNVAERRRDASYYDLLASEARLCSFVAIAQGQLPQE
                     SWFALGRLLTTTGGEPILLSWSGSMFEYLMPLLVMPTYQQTLLDQTYRAAVKRQIEYG
                     RERDIPWGVSESGYNMVDAQLNYQYRAFGVPGLGLKRGLGEELVIAPYATSLALMVAP
                     EEACLNLQRLTAEGADGPYGLYEAIDYTPSRLPRGQSRVIIRSYMAHHVGMSFLSLAY
                     LLLDRPMQKRFEADPLFQASTLVLQERIPKATAFYAHSTELSDLRTTSGTADTPVRVL
                     TTANTPVPDVLLLSNGRYHVMVTNAGGGSSRWKDLAVTRWREDSTCDNWGTFCYLRDV
                     ESGEFWSTAYQPTLKPSKTYEVIFSEGRAEFRRRDHEIETHAEIVVSPEDDIELRRIR
                     LTNCSGTKRTIDLTSYAEVVLALPAADALHPAFSNLFVQTEIIRERQAILSTRRGRSQ
                     DEHAPWAFHLMAVRGPHSGEISYETDRLQFIGRGRTLAAPQALDHSGPLSGSQGSVLD
                     PIVSIRCPIVLEPEESVTVDLVSGIGETRDLCLRLVDKYQDRRLADRVFELAWTHSQV
                     VLRQLNTTEADAQLYGRLAGSILYANASLRAEAAVLSKNRRGQSGLWGYAISGDLPIV
                     LLQIGDPSNIDLVRQMVQAHAYWRLKGLAVDLVIWNEDHAGYRQVLHDQIMGLISAGV
                     EANVMDRPGGIFVRTAEQMSVEDRLLQQSVARVILTDRRGTLADQITRLASPAAPAYR
                     FKPTRTHRADPPATIEPRTDLLFYNGLGGFSSDGREYVITTTAEQVTPAPWVNVLANP
                     NFGTVVSENGLAYTWSENAHEFRLTPWHNDPVTDSSGEAFYLRDEESGHVWSPMPMPC
                     RGTNPYVSRHGFGYSCFEHTEGGIRSEVWVYVALDASVKFTVMKLRNESGRPRRLSAT
                     GYVEWVLGDLRPKSVMHVISEIDPATGALFARNPYNTDFPGRIAFFDVDEGTRSMTGD
                     RTEFLGRNGTLRNPASMSRSRLSGKVGAALDPCGAIQLPFDLAVGQERDCTFRLGVGK
                     DTEDARQLVRRFRGATARRAALETVWHHWTHTLGAVHVETPDQSLNVLANGWLLYQTI
                     ACRLWARSGYYQSGGAFGFRDQLQDVMALVHAKPHLAREQLLLCAARQFKEGDVQHWW
                     HPPSNRGVRTRCSDDFLWLPYVTSRYVMTTGDTGVLDTPIQFIEGRPINADEDSYYDL
                     PGRSEQSGSLYDHCVRAILKGLTKGVHGLPLIGSGDWNDGMNMVGDRGQGES"
     assembly_gap    21983..22082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22083..24853)
                     /locus_tag="PKK81_03100"
     CDS             complement(<22083..24853)
                     /locus_tag="PKK81_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31887.1"
                     /translation="MSRTDILASWRQLTGRLSGQPATMPYAGDELPLRSELFSAEQMQ
                     QHGKTLAGLHAVSAEHEPDPLLARLAENEAVLLDVRNLVAEAVKGSRRITPAGEWLLD
                     NFYLIEEQIRLARRHLPKGYSRELPRLGNGPSAGLPRVYDIALEIISHGDGRVDPESL
                     SGFVAAYQTVTALKLGELWAIPIMIRLALIENLRRAGARIASDRVDRNRAHEWAGQMM
                     ETAEKDPKSLILVIADMARSNPPMVSAFVAELARRLQGQSAALALPLTWIEQRLSESG
                     LTIEQLVQSETQQQAIDQVSMSNSIGSLRFLAAMDWREFVEAQSLVEQTFHEDPAGVY
                     GQMDFATRDRYRHAVEKLAKSSPLSEGDVARQAILLAQTGAAAEGPYHRTAHVGFYLI
                     DNGQTQLEQAVSLRRSAFDRLRRWTGSSSVPIYLGSILFLTATFTGCFLALAIAAGWN
                     GWLLALLGFVSALAASQLAVTLVNWIATALVTPYPLPRMDFSDGLPPALRTLVVIPTL
                     LQDVRGIDDLLEALEVRFLANRGSHLHFALLTDFQDADQETVAGDDQLVLAAGAGIDA
                     LNAKYAGEDGDTFFLFHRPRRWNAHERLWMGYERKRGKLADLNGLLRGGSTDRFSTIV
                     GQPDILSTVKYVITLDTDTQLPRDSARQFVAAMAHPLNRPQYDPAAQRVTAGYGILQP
                     RVAVSLPGTNRSRYARLNAGEPGIDPYTRAVSDVYQDLFGEGSFIGKGIYEVDAFEQA
                     LSDRFPENRILSHDLLEGSYARAGLLSDVQLYEEYPARYNTDVVRRYRWIRGDWQIAR
                     WAFPRVPGPNGRTRSNPLSTLSRWKIFDNLRRSLVPATLTSLFVVGWTLLSPVWLWTL
                     ATLSLFLIAPLLGAVVDLCRKPEDMRMSQHLTATARGMTQQLTQALLTLTCLPYESFY
                     SLDAIVRTAG"
BASE COUNT         4929 a         7037 c         8104 g         5178 t
ORIGIN      
        1 gaccggtacg gcttcataga gggagcatgc tcaaacgcca ctcccaattt ctcaaaagtc
       61 tgctgttttg tatcgatctc gggctgatct gcgcctgctg ggtcggagcc tactaccttc
      121 gtttttcaga gatcgtggaa cctgccacca agggcattcc tcctctccgg atctatctgc
      181 tgttgttggt gccgatcatc gcagtctggg gcatgtcgtt ccaggccttc gacctctacc
      241 gcccgcgacg gatgggcacg cggttgtcgg agtttctcga cgtggcgaaa gccaataccc
      301 tctccgtgct gattctggtg gcattgacct ttttcttccg gcagtacgaa tattcccgac
      361 tggtgctcct ctatttctgg ctgttgaacc tcatcaccct gggcttttcg cgggtgctgt
      421 tccgggaggc gcttcggttc ctgcgtcggc acggatacaa tcaacgccat gcgctggtca
      481 tcggcaccag ccggctggga cgccgggtgg tggacgcgct cgctcagcat ccggagctgg
      541 gggtgaaagt gcatgggttc ttgagcgccg attcccggaa ggtgggtgag cggatcaacg
      601 gggtgccggt gatcgggcgg tacgatgacc tgcaggaacg tgttcagtca ggtgtcgata
      661 tcgtctttgt ctgcctgcct ccggaggacg agcagtgggc tgaaaaaatg tttacctgtt
      721 tggccacgac gatggtcgaa gtgaaggcct tgcccgcgat gtgcgaattt gtgagcctgc
      781 gcgcggaggc cgagatgttc gagggccttc cgctcatcac gttgcaggga tcgccgttgt
      841 acggttggaa tctggtcatc aaacgggtgt tggatgtggt cggcgcatcg gccgcattgg
      901 ttgtattctc cccggtgtta tgcgcaatcg cggtcttggt gaaactgact tcgcccggtc
      961 cggtcttttt ccgtcaactc cgcatgggat tggacgggca ggcgttcgaa atgctgaaat
     1021 tccgttccat gaagctcaat gcggagtcgg aaaccggccc ggtctggacg caacccaatg
     1081 acgatcgccg gacaccgatc ggcgcgtttc tgcgccgaac cagcttggat gagttgccgc
     1141 aattctggaa cgtgctgcgc ggggagatga gcatcgtcgg tcccaggccc gaacggcccg
     1201 agttcatcgc ccggtttcga gaaacacttc cgcagtacat gctgcggcac aagatgaaag
     1261 ccgggattac cgggtgggcg cagatcaacg gatggcgtgg caacacgtca ctggaacggc
     1321 ggatcgagca cgacctgtac tacattgaac actggtcgat ctggttcgac ttcaaaatca
     1381 tgctgctcac ggtctggcga gggtttatcc accggcatgc gtattgatat tttgtgccgc
     1441 cgtgcccaac cggctgcttc cggccggagc ggcattgggg cgcgccggac actccgacat
     1501 ccccgagttt tcttgagttt cacttgatgg cttgttaaca tcggcctcaa ttgaaagcca
     1561 tcgcatgccc agcacaacac gccgcacaaa aacttccgcc aagcctcgtt tctcgcttca
     1621 agaaacactt cgaggaatcc ggttatttgc gacggatgtc gatggggtct tgaccgacgc
     1681 cggcatgtac tattccgagt ccggcgacga gtggaaaaag ttcaatacgc atgacgggat
     1741 ggggatcaaa ctcttgcaga aggccgggat tctgaccgcg atcattacgc aggagtcgac
     1801 aaaaatcgtg atgcgccgcg cgcaaaagct gacgattccc gaagtgcacc agggtgtgta
     1861 tgacaagctc gccgtgctca aggagctgat tgcacggcat ggcttctcga tggatcaggt
     1921 ggcctacatc ggtgacgatg tgaacgatct ccaggccctg ggtgcagtcg gattttcagc
     1981 cgccccggcc gacggaatgc cgccggtgct gaagacggtt cgatatgtgt gcaagaaaaa
     2041 gggcggagag ggcgccgtgc gggaagtggc cgatctcatt ctggcggctc ggcagccacg
     2101 ctgagctatt caccatcacg ctatgtcgaa gatgaagggc gctccatgga tatgacggaa
     2161 catctctatc cggttattct ggccggcggc agcgggacgc ggttctggcc gctaagccgc
     2221 catctctatc cgaagcaatt gctacggatc atcggcgacg agacgctcat tcagcagacg
     2281 atgcgacgcg tcctctcctg cgcgaagccc gagcaggtca tcatctcgac caatccgggc
     2341 caggcggatt cgattcgggt gcaattggga gagtggaagt ccgcgttgaa aaataactac
     2401 gtggtcgagc cggtcggtcg caatacggcc cccgccattg cgctggtggc tgccgagctt
     2461 ctgctgcgcg atcctcatgc gatgatgctg gtcttgcctg cggatcatgt cgtgaccggt
     2521 gagaaggcgt tcaaggcggc ggtggcgctg ggtgtccagc tggccgagca aggacatctg
     2581 gtgacgttcg ggatcaagcc gaccagaccg gaaacggggt atgggtatat tcagcccaac
     2641 cggcggaagt ctctggccaa gaagggacgg ctgacgggcc atccggtcgc gcgatttgtc
     2701 gagaagccga accggaccaa ggcggcacag tacttgaaga acggtcactt ctattggaac
     2761 agcgggatgt ttctctggaa ggccgccact attctcgaag aaattcagca gcaccagccg
     2821 caactggcca aggcgattca gcgggtccat gctctcatgg cctcgggtgc tgagccgggg
     2881 ctggtcgagg cagcctataa gaaagtgccc tcggtgtcga tcgataacgg ggtcatggaa
     2941 cagtcggcca atgcagcgat gattcccgtc ggattcggat ggtcggacgt gggcaattgg
     3001 agcagcctcg aagaggtcgc gccacgcgac aaggccggga acgtcgtcag cgggcgcgtc
     3061 atcgatatgg acagcaccaa ttccgtgctg tacgccgatc gccgggtcgt cgcgaccatc
     3121 gggctttccg acatggtggt ggtcgatacg ccggatgcga cgttgatctg tccgaagtcg
     3181 cgctcacaag atgtgaagca gatggttgag atccttaaac agcagggagc tcctgaacac
     3241 ttggagcact tgaccgtgtt tcgtccgtgg ggatcctaca cggtcttgga agaaggccct
     3301 gggtacaagg tcaagcgggt gacggtgaat cccggagggc gtctgtcgct gcaactccat
     3361 cataagcgca gtgagcactg ggtcgtcatc gccgggactg cgcgggtcac gagaggcgaa
     3421 gagctcctgg atctgcgggt ggggcagagc acggccatcc cggtcgagac gcctcatcgc
     3481 cttgaaaatc tcggttcgga gacgttgcat atcatcgaag ttcagaacgg cccctacctc
     3541 ggggaggatg acatcgtgcg ttttcaggac gcctacggcc gaacctccgt acggtgacgg
     3601 gtgactggaa aattggtgac gtacgtgccg tgaagaatcg tcagatcacc acatcgaacc
     3661 tcggactcgg agtaatgcat ggcactgttt cgtgaatatg atttgagggg cgttgtcgga
     3721 gaagaactca cggaagccat cgccgagcag gtggggcgtg cctacgccac catggcacgg
     3781 gaacagggcg cctcgcgaat cagcctcggc cgggacgggc gattgagttc gcccggcttg
     3841 cagcaggccc tgctgcgtgg cctgctggca ggcggccttg atgtggtcga cttggggctg
     3901 tgcgcctcgc cgctcctgta cttctcattg ttcaccttgc cggtggacgg cggcatcatg
     3961 atcaccggca gccacaatgc cgctgaatac aacggcttta agatctgcat cgggaaagaa
     4021 gcgattcacg gagaggagat tcagcggttg cggcgggtca tggaggccgg tcaatttacc
     4081 accggttccg gacgactctc ctctcatccc atcatcccgg actacttgga gcatctcaaa
     4141 acgaattttt ccggcgtgcg ggcggaccat ctccatgtcg tgatcgattg cggtaatggc
     4201 gcggcttcgt tggtggccaa gcaggccctt gagcagatgg ggtgtcgcgt gaccggcctc
     4261 tatgacgagt tggacgggcg attccccaac caccatcctg atcccaccgt cgtggaaaat
     4321 ctgcaggatc tgatcgcgac ggtgcgggag aaaaaggcgg atgtcggcat cggctacgac
     4381 ggcgatgccg atcggatcgg cgcgatcgac gagcggggag agattctttg gggcgatcgc
     4441 ctcatgatcg tctacgcgcg ggatatcctg acccagcgcc cgggcaccac ctttatctcc
     4501 gaggtcaagg cttcgcaatg cctgtacgac gatattgccg ccaagggcgg gcgtccgatc
     4561 atgtggaaga ccggtcattc cctgatgaag gccaagctga aagccgagtc cgcggtcctg
     4621 gccggtgaaa tgtcgggcca tatgtttttt gccgatcggt atttcggttt cgatgatgcg
     4681 atctatgcgt cctgccggtt ggttgagatt ctggccaaga ccaagcggtc gctctccagt
     4741 ttggtcgccg accttcccca gaccacggtg acgccggaga tccgggtcga ctgcccggat
     4801 acgctgaagt ttcaactggt cgatcaggtg cgcacgcaat tggccgccta tctcgctgag
     4861 ggccagcccg tcggtgcgtc gaatcttcgc atgcgcgagt tggtgacgat cgacggggtg
     4921 cgcgcgattt tcgaagacgg gtggggcttg attcgagcct ccaataccca gcctgcgttg
     4981 gtgctccgct ttgaggcccc gtcgccggcc cggctggatg tgattcgcgc gacgattgaa
     5041 accgagctcg cgcaggcacg acgtgtgctg tcgacgtagc gtgtcccgct attctgcctc
     5101 cctccgtctc agcctgttcc tggccgtcac gatcctggcg acagtcgttg cctctgcccc
     5161 gttgctctct tatgcaacac cggacgggca gatcctcact cctgccgcgt tgccctttgc
     5221 aaacggcgag cgattgtcct acgacgtgac ctggttgggc atgcgcgcgg gaatcgcgac
     5281 catgatcgtt caggagggcc cggtcgaggg gccccggccg cagctcactt ttgccatgac
     5341 ggctcgatcg agcccgaccg ttaccaagtt ttatcccgtc gataaccgcg gggtatccgt
     5401 ggtcgatcgc gaatccttcc tgccccggca catgacgttt gccaggaggg agggcaagcg
     5461 gttcaacgat ttcgactata cgttccggca taccgaaggg ctggttactg ccgtcaaaga
     5521 cgggaaaagc gacgaattgc cgatcccttc cgatgcgcag gacgccatgt cgtgtctgta
     5581 ttatgtgcgc aaggtgctgc ccttcgtgcc gggggcctcg ctggcaatga cggtgcatca
     5641 cgacaaaaag aactacaaga tggacgtgcg ggtcgaagcc cttgaaacag tggagggggc
     5701 gtggggaaag cggcagacgg cccgggtggt ggtcatcatg ccgtttcagg gcattttcct
     5761 gaacgagggc aatattcgcg tctggttcac gaacgatgag catcgggtgc ccgtcagaat
     5821 gaaggccaag gtcgtggtag ggtcgattgt ggccgaattg accgacgggt accgaccgac
     5881 ctcacaaccg taacctgcct gccttggttt gctcgtccgg acggaaaccg ctataatcac
     5941 gccgagttcg tggggagggc ttcggctctg tccgaagccc tcttcatttc gaccggtcgt
     6001 cgttcagttc acgaggaacc ctgagcatgg cgaaatttcc cattacattg agccgcttta
     6061 ttatcgagca acaggccgcg catcccgaag cgaccggaga attctctgtc ctgctgaccc
     6121 aaatcggcct cgtcggaaaa atgattgcgc atgacttgcg cagagccgga ttgaacaaga
     6181 tccttggcac caccggggag accaatattc aaggtgaggt ggtcaagaag ctcgatcaga
     6241 ttgccaatga tacctttgtt cgcgtgttcg agcacagcgg tctggtgtgt gtcttggcgt
     6301 ctgaagagat ggaaaaacct ctgaaaatgg ttcatgaagg agccaagtac atgctcttgg
     6361 tcgatcccct ggacggatcc tccaataccg atgtcaatat gccattggga gccattttct
     6421 ccatccgcag atcgcagctg gggcagcgac agtctgtgga ggacgagttg cttcaacggg
     6481 ggaccgagca aatcgccgca gggtacgtgt tgtatggggc gagcaccatg ctcgtgttta
     6541 cggtcggcca gggcgtgcat ggcttcaccc ttgagccgtc catcggtgaa tatcttctgt
     6601 ccaacgagaa cattcgaatt ccctcaaggg gcaaggtcta ttcggtcaat gagggcaact
     6661 accatcgttg gcctgccggt acacagaaat atctcgattc gctgaaagtg caggacaagg
     6721 cgaccgggcg tccctatagc ggacgatata gcgggtgtct ggtcgcggat gttcatcgca
     6781 tcctcctggg tggcggcatc tatttgtatc cgggtgagac tgccaagccg gaaggaaaac
     6841 tgcgattgat gtatgaggga aatccgcttg cgatggtcgt ggaacaggct ggaggaaaag
     6901 cgacgacggg gacgatgcgc attctcgacg ttgaaccgaa agccctccat cagagggtgc
     6961 cattgatcat cgggagtgcc gaggatgtcg ctcaggcgga agcctatatt caaggacgtg
     7021 cgtaacgact catgtgactg aggtttgttg ggaggatgat tatgagcaat cgggttcaag
     7081 aaattctgag ctggtacgag agtgacaatg ccggaacgaa gacgaacatt gcgcggctgc
     7141 tgaatcacgg caagctggcg ggcacgggga aattggtgat cctgccggtg gatcaggggt
     7201 tcgaacatgg tccggccaga agttttgccc ccaatgccgg cggctataat ccgcattacc
     7261 atttccagct ggggctcgat gccgggtgca acgcctacgc ggccccactc ggattcatcg
     7321 aggccggtgc cggccatttc gccgggcaaa taccactcat cctcaagctc aacagccacg
     7381 atacgctcca tgacgagaag gatccgatgc cgtctgtgac gggcagcgtg cgagatgcct
     7441 tgcgattggg ttgttcggcg gtgggattca cgatttatcc gggctcgtcc cactgcaatg
     7501 cgatgtatga acagttgcgc gcgattgcgg aagaagccaa gagttgcggg ctggccgtcg
     7561 tggtctggtc gtacccgcgc gggtcaggat tgagcaagga aggcgagacg gcgttggacg
     7621 tcgtggctta tgccgcccag attgcggcgc agttgggcgc ccatattatc aaggtcaaat
     7681 tgcccacggc ccatctggag caggcggctg ccaagaaggt ctatgaagct gaaaaggtgc
     7741 cgatctcgac cttggcggaa cgggtcaaac atatcgtgca aagttcgttc gacgggcgcc
     7801 ggatcgtcat cttctccggc ggcgcgaaga gcgacgaaaa gacggtgttc gaggaagtcc
     7861 gtgcaattcg tgatggcggg ggattcggtt ccatcatcgg ccggaattcc ttccaacgtc
     7921 ccaaagccga ggccgtcaag ttcttgaaca ccatcatggg actgtatgcg ggagagaagc
     7981 cctaaccggc gcctccgtcc gcacggatca caatgaacga ctttcagatc ccaacggagc
     8041 agcagcctcc gaggcggtcc tggatatttc ccgcactgct cttgatcgcg ggggtattga
     8101 tcggggtcat tctgacctcc gatctcggct ggttgccgac cggacatgcg gttccggaac
     8161 cggttcctcc tccggtcgca accccggtct ccacggcggt gcagcccacg ctgcccggcg
     8221 cgggtgggca gagtttcgtc gatgtcgcta aagtcgtgaa gccggccgtc gtgaacattt
     8281 ttgcgacacg gaatggatcc agcgaggagg ggaagggcac cccgtttgag gatccatttt
     8341 ttagacgatt tttcggcgaa gaatggatga agcggttcga ggcgccgaag gaacgcaagg
     8401 aacgagggtt ggggtctggc gtcatcgtcg atccgaacgg tctcattatc accaacaatc
     8461 atgtggtgag caaggccgac gagatcaagg tcttcctctc cgacaagcgt gagttcaagg
     8521 ccaagctggt cggcacggat gccaagaccg acgttgccgt gctgaagatc gaggccggtg
     8581 gattgccgac cgtcgcctgg gccgattccg acaagctgga ggtgggtgag tttgtgctgg
     8641 cagtgggcaa tccgttcggg ttgacgcaga cggtgacgct ggggatcgtc agcgcccttg
     8701 gtcgcgcggc cggcatcgcc gaatatgaag acttcattca gaccgatgcg gcgatcaacc
     8761 cggggaactc cggcggcgct ctggtgaatg tgcgcggtga actggtcggt atcaatacag
     8821 cgatctatag ccagagcggc gggaacatgg gaatcggctt tgccgttccg agcaacatgg
     8881 ctcattccat catggagcag ctggtccagc atggcaaagt cgtgcgcggc tggttgggtg
     8941 tctcgattca ggaattgacg ccggaattat cttcgcagtt cggcgtgccg aaagacgtga
     9001 agggtgtcct cgtcagcgac atcatggacg acagtccggc gaagaaggca ggttttgagc
     9061 gcggcgatgt gatcgtcgaa tacgacggga aaccgatgga ttctccggct cacttgcgga
     9121 atgccgtggc tcagacggta gtggggaaga aggtgacggt caagctcatc cgcgaaaaga
     9181 agcccaagtc catcgaactc acgattgctg agcagccgaa gaatctggca caagccgggg
     9241 ttgaagaggg cggagaatcg gtggctcccg ccggcttact ctcggacgtc gaggtgcggg
     9301 agttgaacac cgaactggcc ggccggtatg gcttgaagtc gagcgatcac ggggtggtgg
     9361 tggtgcgggt taaaccgggg agtcccgcgg aagaagccgg tgtgcgtgaa ggggatctcg
     9421 tactggaggt gaatcgcaaa gccgtgggga cgttaaaggc gtatgaacat gtcgccgcga
     9481 atttgccgaa agaccaggcg gtattgctcc tgttgagacg gcaggggcga gccatttatc
     9541 tgacgctgag gccgtgatcg caccgaatcg ttcgttcggg gttggtcaca gagaggaggc
     9601 aaacagtatg gtaacacggg ccatatttgt tcttctcagc gctcttgccg gcatggccct
     9661 gtttcttcgg gcccaggatc caagtcgcga gtttttgttg atgggattgg gaatgggagg
     9721 ggtggccggt gggctcatcc tggccggcga gtatgcgctc cgcaggcttt cattcggcat
     9781 tattgtcggc ggtgccgtgg ggctcgccgg tggtcttatt ctgacgggtt tggtggagtg
     9841 ggtcgggagt gcggtcttcg atgtggagac cttcctgttt catatcggcg ggttggtgtt
     9901 tcttttaggg ttgccctatc tggggttggc catgggcgcg cggttcggca acgaacagtt
     9961 ccccggtctg gaccaggggc cgacggcagc cggggcttcc catgccagcc ttaaagtatt
    10021 ggataccagc gtcatcatcg acggccgggt tgccgacctc tgtgagacag ggtttctgga
    10081 agggccgttt ctggttcccc actttatctt gaatgaatta cagcacattg cagactcgtc
    10141 ggattctttg aaaagggcgc gcgggcgtcg cgggttggat attctcaaca agattcagaa
    10201 aatgccgggc atcgatgtcc ggatcatcga cgaagatttc ccccatgtga aggaagtgga
    10261 tgccaagctc gtggttctgg cgaagaaagt cggggggcgc atcgtcacga atgacttgaa
    10321 cctgaacaaa gtcgccgaat tgcagggcgt gcgagtgttg aacatcaatg agctctgtaa
    10381 tgcgctgcgt ccggtcgtgt tgccgggtga aaccattcgg gtcttcgtct tgaaggaagg
    10441 caaagaggcg ggccagggcg tcgcatacct ggatgacggc acgatgattg tcgtggacaa
    10501 tgctcgacgt tgtatcggcc gtaatgtgga tgtgactgtg accagtgtgc ttcagacgac
    10561 ggcggggcgc atgatcttca cccgtttgaa ggaagagtcc gagcgcgaag agtatcaggt
    10621 tgcgcgtggg tagtttggcg agacggaaga gcggtggccc aatggaatcc tccgtcctcc
    10681 ggggaggcgc atcgagctcc cacgattgcc taggtttggc cttgtcccct tgctgctcac
    10741 gtgagtgtgc cggtgtccgc tcgtaacgcg aagccgtcgc tggcagggcc gcgcacggtg
    10801 gccctggttc cggccgccgg tcgtgggctt cgcatgggcg gccgtatccc caagcagttt
    10861 cttgcgttag gggggcggcc gattctcgcg cagtccctcc ttgtgctcca agcttccccg
    10921 ctcattcacg agatcattct ggcggtgccc gagtcggaac gtcagtattg cctggatcat
    10981 atcgtggcga cgggtgagtt cagcaaggtg accaaggttg taccgggggg agcgcagcgg
    11041 caggattccg tgcgtcatgc cctggccgag gtgagtcagg agaccgagat ggtcctggtt
    11101 catgacgcgg tgcggccgtt tctcaccgag gacatgatcc gcagagtcgt ggctgcggct
    11161 gtcgagcatg gggcggccat catcgcgctg ccgatgcgcg acacggtgaa gtatgtgggg
    11221 gccggcgggg tgatcgaacg gaccgtagat cgccgccctc tctggttggc ccagacgccg
    11281 caggcgtttc gtcgtgactg gttggaagag gggcatcaca aggccttctt ggccggtgtg
    11341 caggcgacgg atgacgcgca cctcgtggaa ttgatcggaa aaccggtcgt ggtggtcgag
    11401 gggagcggag aaaatatcaa ggtgacgagg ccggaagatc tcgtgatcgg agaagcgatt
    11461 ctcagctcac gaacggcggc aaggagtgcg gaatgacggc tgtacgcgtg ggatgtggat
    11521 gggatattca tccgatggtg gcgggacgaa aattaattct gggtggactc gaaattccgc
    11581 accacaaggg gctgcaaggg cattccgatt ccgatgcgtt agttcatgcc atttgcgatg
    11641 cgttgctcgg ggcgatggga gaaggagatc tcgggcgtca ctatccgagc tcggatcagc
    11701 ggttcaagaa tatttcgagt ctcaagttac tcgaagacgt cgttcagaag ctgcgcgcca
    11761 agggctatcg tctggccaat gtggacagca cgatcattgc acaggcgccc cggttgagcc
    11821 cgcatctggc cgccatgcag aagattattg ccggggtgct tgaagtggat cccgatctgg
    11881 tgaatgtaaa agtgaaaagc ggcgaaggat tggatgcggt agggcgtgaa gaagcgattg
    11941 cggcgcaggc ggtctgtctg atcgagcgag gctagagcct atccgctggt gcgctcacga
    12001 gtctggtacc acctatgttc aagactatct ctcaggacct ccaagcgatt tttgaccggg
    12061 atcctgccgc caccagcagg ctcgaagtca ttgcgaccta tgcgggattt cacgctctgt
    12121 tggcctatcg gctcgcacat tggttgaagg ggcatcgtgt gccgttcctg ccccgcgcga
    12181 tttcccaatt ggcccgctgg ttgacgggaa tcgagatcca tccttccgcc aagatcggtg
    12241 agcgattttt tatcgatcac ggcatggggg tggtgatcgg agaaacggct gaggtcggag
    12301 acaacgtcac cctgtttcag ggcgtgacgc ttggtggaac ggggaaggag cgcggcaagc
    12361 gccatcccac gctcggcagc catgtggtcg tcggggcggg ggcgaagatc ctcggcggga
    12421 ttacgatcgg cgacaacgtg aaaatcggcg cgaattccgt cgtcttgaag tcggttcccc
    12481 ccaattcgac ggtgatcggg gtgccggcca ggatcatcaa gtcgcaaggg gagcggctcc
    12541 ccgatgcgac catggaccat accaatatgc cggatccgac cgccgatcga tttgccgcgc
    12601 tcgaactgga actgatcgag ttgcgaaaga agctcgaaaa tcgagacccc aacagccccc
    12661 ggtagcatcc cctcactcct ccgctgacga acccatctat tcccgttgtg ttgagatacg
    12721 taacgtaggc ctgtggtaga cagtgcaggt gaatgaccgg cgggctgtcg agccccggcg
    12781 cggccgtgcg gtcgcgccgg ggcgctacga ctagttggac aggcactcct tcataaactt
    12841 cttgcgctcg tcgccggcca gtttcttggc cttggcatcc ttgttacagg tcgtcatctt
    12901 gttctgttgt gaggcaccgg cagcggcttt ctccggttta gcggagagac attccttcat
    12961 gaatgccttt cgcgcgtcac ccttgccctc cccggtgagt cccttggcat ttgcatcggc
    13021 attgcaggtt ttcattttgt tctgctgtgc gccggcgtag ctgagctgcg gcgtggcgag
    13081 tacggctgcc aacatgagaa cggacaaggt cgtacagagc agtttcatgg cgggctcctt
    13141 tcaaggtgaa cgtgggttgt gtgcggaact ataacaaacg gcgcccgacc tgtctcgcgg
    13201 gggagttcct taagggagag aggggatgct cccttcggta tagtcaactc ggacgagata
    13261 gagcccgtga gcgggggcag ttctcccggc cgcggccctc gcccgcgcgg caagcacggt
    13321 gcgcatgtcg ggggcggtgc gcttgccctg ccccacttcc accagggttc ccaccatggc
    13381 acggaccatc tgcttgagaa accggtcggc atagaaggag agaatgatga gatctccctg
    13441 gcggcgcaga tccgcctgtt gtaaccggca ctgcggattc tcgttatcgg tcggcgccgt
    13501 ttcgaatgag gagaaatcgt gcgagccggt caggcaggcg gcggcttccg tcatggccgc
    13561 aaagtcgagc ggtttataga gcatccaggc tcgttcccgg agcaggggtg ctcgctcagg
    13621 ccgattcatc agatgatact cgtacagctt ccctttcgct gagtagcgcg catgaaaatg
    13681 atccggcacg gcttcgaccg agatggcgct gatatcggcg gggaggtgag cgttcaacgc
    13741 gcgaagccac tcgcgttggg agagcctgcg atcggtcctg aagctggcga cctgtccgaa
    13801 ggcatgaacc ccggcatcgg tccggcccgc tccgacgagc gtgagcctgg tttgggcgat
    13861 tgctgcgagg gcatcttcga ccgttgcctg gatggtcggc acgttcagct ggcgctgcca
    13921 cccagcgtac tgtgtgccat cgtactcgag gacgagttta aatgtggtca tactctggaa
    13981 ggaaaagaag gaactctagc ccaacgggaa ggcgcctgtc atctgggggg attggtgtgg
    14041 aggtaggtct tgaccccttc gaaaatcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnngc ctgtcatctg gggggattgg tgtggaggta ggtcttgacc ccttcgaaaa
    14221 tcgactccgc aatgtgcgcc aggaagggct gtgaccgcat ctgctcttct tcggcgggat
    14281 tcgacatgaa ggcgatctcg gccagaatgc tgggcatggt ggtgaagcgg agcacgtaaa
    14341 atggcgcggt cttcaccccg tgatcgatgg tgctgtaacg accgttgaga tttgtgacca
    14401 tcgcctgctt ggccgtccag gcgagttctt gggagtgttc gattttcttg gatgtcagga
    14461 ggtcggcgac gatgtattcc cagcccactc cggtattgtt caacggcgtg ccgttttctc
    14521 gcgcggccac ttccagggcg cgctgatcct tcgcttcgcc gaaatggtag atttccagac
    14581 cacggacgcc cttgtgcggg tgtgaattga cgtggatcga gatgaacaga tccgcgtctt
    14641 tgccgttcgc aaatttggcc cggtcctcca gctcgataaa ggcatcgcgt tcgcgggtca
    14701 tcaggacgcg tgtattcggc acggtactga gcaaggattt caacagcagg ccgaccttta
    14761 aggtcacctc tttttccgtc gtcccgtgct gtccgattgt gccgggatct ttgcctccat
    14821 gcccggggtc aatgacgatc gtctggaccg ggcttgccgg cttgggcgca accgggcgag
    14881 ccggttccgt gacggtgggg cttggaatcg ccggcggagt cggcaagggg acggccgggg
    14941 cctgtggcga tgtcgccgag gtctcaccga tctgccggat ctgatctttt ggacgctgcg
    15001 taatatcgat gacgaggcgg tcaggattat cgagcgagaa caccttgtag cgggaattct
    15061 gggtacgcgt cagcgtgatg ctgacgacgc gcgggcggct ttgcgcaatt tggaacgatt
    15121 gcgggatcgt cccgcccgag acgcgctgcc gtgaggattt tccaaggatc gcgttgttca
    15181 cctcaatgag cacccggtcg gggttgcgca aatgcgtttc ggagaaggac gtcttgtgat
    15241 tcaggtcgag cacgaggcgt gtgccatccg cggtggacca ggtgcgcagg tcgcggacga
    15301 tgatcggata ccgtgacgcc ccggcgcgct cttgcttgac caggaggaaa cggggggagg
    15361 gctcttcagc ggcaacctgg ggaaccgtcg gccaggaggg ttcggtctgg atctcgccac
    15421 ccgatgagat ccccgtgccg aacaggacgc aggcgaacgc gaacgtcagg aggtgacgga
    15481 tcaaggaggc gacatgggac acgaaccgga actcctctcg ataggatacc ctctaggcct
    15541 tttctcagat gtttatggta atgtcaacaa aaggcctcaa accatttaat tgtagatggc
    15601 aacacatctt cttgtcgatg ggtataacct tgtgggcagc gccggaatgg ccctcccggc
    15661 tggttccgga cgccttgagg cggcgcgcga gacgctcctg aagaacctgg ccgggtaccg
    15721 tcagagaaaa ggccatgcga ttaccgtggt cttcgacggc tggcagggcg ggctgggcgc
    15781 ggaacatcgt gaatttcaat ccggcgtcga agtcgtctat tcaaaacgag gcgaacgcgc
    15841 cgaccaggtc attcaacgtc tcgcccgtct gtacggcagg gcctgcgcgg tggtgtcctc
    15901 ggatcatgaa gtggtcaatt cagccagggc ggccggcgcg ttcgtcatcg gggcggcgga
    15961 gtttcgcgcc aagttgcagg atcgatcgtc cagcctgtcg accgtcgcct ttaaagaact
    16021 ggatcgcggc gatctcgatc gcgtgaagcg atccaaagac aagggcggga atccccgcaa
    16081 gctgccgaag tcacaacgta aacgaaacca tcaacttagg gaattttgaa caggcggcgg
    16141 gcgttctcgc tggtacggcg ggccacgtct tcagcggtcg aggggacgtt gtcggcggtg
    16201 atcgtcgcca gcagttcggc cacatgcctc acaaaggccg gttcgttgcg cttgccgcga
    16261 tgcgggaccg gcgtgaggta ggggcaatcg gtttcaatga gcagccgatc cgccgggacc
    16321 gtcttggcaa tctcgcgcaa catggtggca ttctgaaagg tcaggatgcc ggagaatgac
    16381 agataaaatc ccagctcgat cgcatccttc gccagccagg catctcctga aaaacagtga
    16441 aacaccccgc cgatttctga ggctcgctct tccttcagaa tgcgaatggt atcgtcctgc
    16501 gcttccctgg tgtggatgat gaccgggagc tttaactcac gtgccagctg aatttgctca
    16561 cgaaagcgcg tccgctgtaa ttccggatcc gagtggttgt aatggtaatc gagaccgatc
    16621 tcgccgtagg ccaccacctt cttgtcgcgc gccagccgcc gaaactcatc gtaccaaccg
    16681 tcctcgatgt gtttgacttc gtgggggtgg acgccgatcg acgcgtagac gaaggggtac
    16741 tggttggcga gggtgaccgc cgatcggctg gtggcaaggt cgcagccgat ggtgatcatc
    16801 gattccacgc cggccgctcg cgcacgggcg atcatggcct cgcggtcgga ctcgtagcgc
    16861 gcatcgtcga gatgggtatg ggtatcgatc agcatggggc gcatcctaac atggttgtcc
    16921 gctcgtagga tgctgaaaaa gtttgccgat ggcgctctct acacccgtga agcgtctctc
    16981 gtccgcagaa aagagcctct ggcgtatagc tcatagcgta tgatccgaaa tagagagtgg
    17041 gcgtctttac taccagctat aagccatcag ccatacgctc ttattcccac cgagatacgc
    17101 ttcacgaacg acggacgaaa gggaaacctc gggagccacc agccaccaga gcgtcccggt
    17161 atgccaacgc gctcagttct ctcacgcgga tgggatggtc acctcagctg aatgttctcg
    17221 acgatcatcc acaagtcgaa tcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnngctttc tccctggccg cgatcgccga ccatattcat cccgtcgttc caatcacctg
    17401 aaccgatgag cggcagaccg tgtacaccct tcgtgagccc cttcaggatg gcccggacac
    17461 agtggtcgta gagactgcct gattgctccg accggccggg aagatcgtag taggaatctt
    17521 cgtcggcatt gatcgggcga ccttcgatga actggatcgg ggtgtccagc acgccggtgt
    17581 ccccggttgt catcacgtag cgactggtga catagggaag ccagagaaag tcatcggagc
    17641 agcgcgtgcg cacgccccgg tttgacggtg gatgccacca atgctgcacg tctccttcct
    17701 tgaactgacg ggcggcgcac agcagcagct gttcccgcgc gagatgcggt ttcgcgtgga
    17761 ccaaggccat cacgtcttgc aactgatcgc ggaatccaaa cgcgcctccc gattgatagt
    17821 agccgctgcg cgcccaaaga cggcaggcga tcgtttgata cagcagccag ccattggcaa
    17881 gcacattgag cgactgatcg ggggtctcca catggaccgc tcccaacgta tgtgtccaat
    17941 gatgccagac ggtttccagc gccgcacggc gtgccgtcgc ccctctgaag cgacgaacga
    18001 gctgacgggc atcctcagta tcttttccaa ctcccaaccg gaaggtacag tcgcgctctt
    18061 gaccgacggc gaggtcgaag ggcaactgaa tcgcgccaca cggatccaga gcggccccca
    18121 ctttcccgga cagtcgtgac cgactcatcg acgcaggatt gcgcaaggtg ccgttgcgtc
    18181 cgagaaattc ggttcgatcg ccggtcatgc tccgagtgcc ctcgtccaca tcaaagaaag
    18241 caatccgccc ggggaaatcc gtgttgtagg gatttcgcgc gaacagcgcc ccggtggccg
    18301 ggtctatttc actgatcaca tgcatgaccg atttgggccg cagatcaccc agcacccatt
    18361 ccacatagcc ggtcgccgac aggcgtcgtg gtcggcccga ttcgttgcgc agcttcatca
    18421 ccgtaaattt tacggaggca tccaacgcta catacaccca gacctcggag cgaatgccgc
    18481 cttccgtatg ctcaaagcag ctgtacccga atccatgccg actgacatag gggttcgtac
    18541 ccctgcaggg catcggcatc ggcgaccaga cgtggccgct ctcctcatca cggagataga
    18601 aggcttctcc gctcgaatcg gtcaccggat cgttgtgcca gggtgtgaga cggaactcat
    18661 gcgcattttc gctccaggtg taagcgaggc cgttctctga gacgacggtg ccgaaattcg
    18721 ggttcgccag cacattgacc cagggagcgg gcgttacctg ttcggctgtg gtcgtgatca
    18781 cgtactcgcg gccatcggaa gaaaatccgc cgagaccgtt atagaacagc agatccgttc
    18841 ggggctcgat ggtggccggc ggatcggcgc gatgggtacg cgtcggtttg aagcggtagg
    18901 ccggggccgc aggggaggcg aggcgtgtaa tctggtccgc cagcgttccg cgtcgatcgg
    18961 tgaggattac gcgggccacc gattgctgca gcaggcggtc ttcgaccgac atctgttccg
    19021 ccgttcgcac gaagataccg cccggtcgat ccatcacatt ggcctcgact ccggccgaga
    19081 tcagccccat gatttgatcg tgcaacacct gacgatagcc ggcgtgatct tcgttccaaa
    19141 tcacgaggtc cacggccagc cccttcagtc gccaataggc atgggcctgg accatttgac
    19201 ggaccagatc gatgttggac gggtcgccga tctggagcag tacgatcggc aggtcccccg
    19261 aaatcgcata gccccagagg cccgattgtc cgcggcggtt tttgctcagc acggccgctt
    19321 ctgccctgag tgaggcattc gcatagagga tcgagccggc cagtctgcca tacaattgcg
    19381 catccgcttc ggtcgtattg agctgacgca gcacgacctg gctatgggtc caggccagct
    19441 caaacacccg atcggcgaga cgccggtcct gatacttgtc gaccagacgc aggcacaggt
    19501 cgcgggtctc tccgatgccc gacaccagat ccaccgtgac cgactcttca ggttcgagga
    19561 cgatcggaca acggatggac acaatggggt ccagcacgga gccctgactt cctgaaagcg
    19621 ggccggaatg gtccagagcc tgcggagcgg cgagggtgcg cccccggccg atgaactgca
    19681 ggcgatccgt ttcgtaggaa atctccccgc tgtgagggcc acgtaccgcc atgaggtgga
    19741 atgcccaggg ggcatgttca tcctgcgaac ggccacggcg cgtggagaga atggcctgac
    19801 gttcgcggat gatttccgtc tggacgaaga gattgctgaa ggccggatgg agggcatcgg
    19861 ccgccggcag cgccagcacg acctccgcat aactggtgag gtcgatcgtg cgtttcgtgc
    19921 cggaacagtt ggtgagacgg atccgccgta attcgatgtc gtcttccggg gacacgacga
    19981 tttcggcgtg tgtctcaatc tcgtgatcgc ggcgacgaaa ctcggcgcgc ccttcggaaa
    20041 agatgacttc gtaggtcttg gacggtttca gtgtcggctg ataggccgtg gaccagaatt
    20101 ccccgctctc tacgtcgcgg agataacaaa acgtccccca gttgtcgcag gtgctgtctt
    20161 cacgccaacg cgtgacggcc aggtccttcc atcggctgga cccgcctccc gcgttcgtca
    20221 ccatcacgtg gtatcgaccg ttcgacagga ggagcacgtc cggcaccgga gtgttcgcgg
    20281 tcgtcaggac gcgcaccggc gtgtctgccg taccggacgt cgtgcgcaaa tccgacagct
    20341 cggtgctgtg ggcatagaag gctgtggcct tgggaatccg ttcttggagc accagcgtgc
    20401 tggcctgaaa cagcggatcg gcttcgaagc gcttctgcat cgggcggtcg aggagcagat
    20461 aggccaagga gaggaaactc atccccacat ggtgggccat gtaggagcga atgatgacac
    20521 gcgattgtcc gcggggaaga cgcgagggcg tgtaatcgat cgcttcgtac aagccgtatg
    20581 gtccatcggc gccttcggcg gtgagtcgct ggagattcag acaagcctct tccggcgcga
    20641 ccatcagggc gagggaggtg gcgtagggtg caatgaccaa ttcctcgccc aggcctcgct
    20701 tcagtccaag gccgggcaca ccgaaggccc gatactgata gttcaactgc gcgtcgacca
    20761 tgttgtagcc ggattccgag acaccccagg gaatatctcg ctcccggccg tattcgatct
    20821 gtcgcttcac cgctgcgcgg taggtctgat ccaacagcgt ctgctggtat gtcggcatga
    20881 cgagcagcgg catcaggtat tcgaacatcg atccgctcca ggacaggaga atcggctctc
    20941 ctcccgtcgt cgtgagcaga cggccgaggg cgaaccagct ctcctgcggc agttggccct
    21001 gcgcaatcgc cacgaagctg cacaatcgcg cttcggaggc caggaggtcg tagtaactcg
    21061 cgtcccgtcg tcgttccgcg acgttgtacc cgatggagag gaggaaccgc gtgtcgtcga
    21121 acaggaagtc gtactccatc tgggcaaatt gattcgcttg ccgggccaag tggtcgagcg
    21181 aggccaggcg ttgttcggcc agtcggctgg ccgcgctcag gtgccgtcga agttcgatca
    21241 gccaggtccg ctccgtgccg gatgcgtctg gtccaagtcg cgcgtcaatc gccggcagcc
    21301 attcgccttc cagtctggtc agcgtccgga gcgtggggat ctgatccaat tctggaaaca
    21361 ggcggaggat ctcatcggtg gcggcaagac cgatccaggg agccagaatc atcagctcgg
    21421 cgaccgtgtc acggcactgt ctcgcgaaag catgggccca tcggtgcgcc tccgcgttgg
    21481 aggcgggatc gagatgggcg gtgacttcat ccacctgttt ggtgagcaag gtcaaggtgt
    21541 ggtggatttg cctgagtgtg gtcggcctgt tgtcacaggc tgcggccagg tttttctgaa
    21601 gtgcgtcaac ctgagacgtc ggagtttcga gcgcctcgct cagcagcgcg agggtatcgc
    21661 ggagtccttc aaaggtgcgc tcagcgagga tcttgtcttc cggcaggcta aggagccccg
    21721 agtgcagggt catgacatgc cccgccagat ttccactgtc taccgacgaa atgtacatgg
    21781 ggagcagcgg tttcagtgtc tgggtgtcgt accaattgta gaaatggcct cgaaatcgtt
    21841 ccaaggttgc catgctgtcg aaggtatgag cggtgcgttc gatcagctgt cctgccgaca
    21901 ggtaaccgaa gtcgtaggcg gagagattgg ccaacagcgc caaccctata ttcgtcggtg
    21961 acgttcgatg cgccaccttc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22081 nnccggcggt ccgtacgatc gcgtccaggc tgtagaagct ttcgtaaggc aggcacgtga
    22141 gcgtgaggag cgcctgggtc agctgttgtg tcatgccccg tgcggttgcg gtgaggtgct
    22201 ggctcatccg catatcctcg ggcttgcggc acagatcgac gacggcgccc agtaacggcg
    22261 cgatcaggaa caggctgagc gtggcgaggg tccagagcca gacaggggaa agcagagtcc
    22321 atcccactac gaataaggag gtcagcgtag cggggacgag gctgcgtcgc aggttgtcga
    22381 aaattttcca acgcgacagg gtggacagtg ggttgctgcg ggtacgtccg ttcggtcccg
    22441 gcacgcgggg gaaggcccag cgcgcaatct gccaatctcc gcggatccag cgataacgcc
    22501 ggaccacgtc cgtgttgtag cgggcaggat attcttcgta gagctgcaca tcgctcaaga
    22561 gtccggcgcg ggcataggag ccttcaagca agtcgtggct gaggatgcga ttctccggga
    22621 agcgatcgct gagcgcctgc tcgaaggcat cgacctcata gatccccttg ccgatgaagg
    22681 acccttctcc aaacaggtcc tgatacacgt ccgacacggc gcgtgtgtag ggatcgatgc
    22741 cgggttcgcc ggcgttgagc cgggcgtaac gcgagcggtt ggtgccgggg aggctgacgg
    22801 ccacccgagg ttgcagaatc ccgtaccccg cagtcacgcg ctgtgccgca ggatcgtact
    22861 gcggacgatt gagcggatgg gccatggcgg ccacgaattg ccgcgcggaa tcgcgaggca
    22921 actgggtatc cgtatcgagc gtgatcacat atttcaccgt ggacaggatg tccggttgtc
    22981 cgacgatcgt ggaaaaccga tccgtcgacc cgccgcgcag gagcccgttc aagtccgcca
    23041 gtttgcctcg cttccgctcg tatcccatcc agagccgctc atgggcattc cagcgacgcg
    23101 ggcgatggaa gagaaaaaac gtgtcgccat cttcgcctgc atatttggcg ttcagcgcat
    23161 cgatgcctgc tccggccgcc agcaccaact gatcgtcacc tgccaccgtc tcttggtcgg
    23221 catcctggaa atccgtgagc agggcaaagt gtagatgact gccccggttg gcgaggaatc
    23281 ggacctcgag ggcctcaagg aggtcgtcga taccccggac atcttggagg agtgtgggaa
    23341 tcacaaccag cgttcgtaac gccgggggaa ggccatcgga aaaatccatt cgcgggaggg
    23401 ggtacggggt caccagcgcc gtggcgatcc agttcaccag cgtcaccgcc agctgactgg
    23461 cagccaaggc cgacacgaat cccagcaggg caagcagcca tccattccaa cctgctgcaa
    23521 tggcgagggc caggaaacat ccagtgaatg tggctgtgag gaacagaatg gagccgagat
    23581 agattggtac gctgctggat ccggtccatc tccggagcct gtcgaaggca gaacggcgca
    23641 ggctcacggc ctgttccaat tgcgtctgtc cgttgtcgat gagatagaag ccgacgtggg
    23701 cggtgcggtg gtacggtccc tctgcagcgg cccctgtctg cgcgagcagg atcgcttggc
    23761 gagcgacgtc accttccgac agggggctac tctttgcgag tttctccacg gcatggcgat
    23821 accggtcacg agtggcaaaa tccatctgcc cgtacacgcc ggccggatct tcatggaacg
    23881 tctgctccac caggctctgc gcttcgacaa actcgcgcca gtccatcgcc gccaggaacc
    23941 ggagactgcc gatgctgttg ctcatggaga cttgatcgat cgcctgttgt tgcgtttccg
    24001 actgcaccag ttgctcaatc gtgaggccgg actcggacag gcgctgttcg atccaggtga
    24061 gcggtaaggc caacgccgcg ctctgtcctt gcagacggcg ggcgagctct gccacgaagg
    24121 cgctcaccat gggcggattg gaccgcgcca tgtccgcgat caccaggatg agactcttgg
    24181 ggtccttttc agccgtctcc atcatttggc cggcccattc gtgggcgcga ttgcgatcca
    24241 ctcggtcgct tgcgatgcgg gcgccggcgc gtcgcaggtt ttcgatcaat gcgaggcgaa
    24301 tcatgatagg gatggcccac aactcgccga gcttcagggc cgtgaccgtc tgataggccg
    24361 cgacgaaacc gctgagactt tccgggtcca cccgtccgtc gccatgcgag atgatttcaa
    24421 gcgcgatgtc gtagaccctg ggcaagccgg ccgatggacc gttgcccagc cggggcagtt
    24481 cgcggctata ccctttgggc aggtgccgcc tggccaggcg aatctgttct tcgatcaggt
    24541 agaagttatc gagcagccat tctccggccg gtgtgatgcg tcgacttccc ttcaccgcct
    24601 cggccaccag attccgcaca tccagcagga cggcttcatt ctcagccagt cgagcgagca
    24661 gcgggtcggg ctcgtgctcg gcactgacgg catgcaaccc cgcgagggtc ttgccatgct
    24721 gttgcatttg ctcggcactg aagagctccg atcgcaacgg cagctcatca ccggcatagg
    24781 gcatggtcgc cggttgtccg ctcaggcgtc cggtcaattg acgccagctt gcaagaatat
    24841 ctgtgcggct catcttcaaa ttcgagattc ccttcgatcg aggtcgcgga ggtggtctgc
    24901 gggatacagg caaaggaccg gcccaagccg gacggtcctt gcgaatgaag gtacggaagc
    24961 gggcacaagg aggcagtaga gcctcacccg cacgactcat aacggcttct gctgcaatcg
    25021 ccgatctgtc gctgcgacag accttcgtcc tgcggactcg cccctcaccc gctcaccgca
    25081 ctgcgcgagc gggcccgtgg tcttgggact ccgtacgcct ggcgcagtgc cgctcagtga
    25141 ctttgcggca gaccgtcatg aatcatgcgg gccgaggaat ctgcaagcga acgacgattg
    25201 tgcaatcgac aattcggaca cttcgatgcg agctgtgacg cggatcgaac gcagcgatag
    25261 gaggcgtgct ggatgctgtc ctactctagt ccttttaggc aggtgaaaga cagacaaata
    25321 tccgcaggct gtgcggctgg agcgtcgaag ggagttcttg aggtgcacga cgagcacggg
    25381 agaaggctga gcagccggcg ctcagtcaga ctcactggtt ggaggcgtag cgtggagggc
    25441 gttcctcagc cgaggagcga gtcgtcgaaa cgaatcagtc ggtgaacggt gggagtgtgc
    25501 actcaaaaaa ttctgcgcag ggcctccgat cgaacatggg tagcctgc
//
LOCUS       DAOQRM010000018        25251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1240_length_25251_cov_15.830489, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000018 DAOQRM010000000
VERSION     DAOQRM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25251
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1240_length_25251_cov_15.830489"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(575..775)
                     /locus_tag="PKK81_03105"
     CDS             complement(575..775)
                     /locus_tag="PKK81_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31888.1"
                     /translation="MHKQVGRIGSVRRELHGKACPFCGGHKYQLVLRSQTSPGTDGLF
                     ARCSQCQRAKELDEDLGRILWM"
     gene            complement(1367..1732)
                     /locus_tag="PKK81_03110"
     CDS             complement(1367..1732)
                     /locus_tag="PKK81_03110"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV31889.1"
                     /translation="MKQRYTKREPVQSTCILSCEGMMGQGQLLNLSVPGCLLKTSMKL
                     KVGQYVDLRLSFPTSATALQVKLAAVRWAKNGQVGLEFIRMSETDQTRLRFLSGHIEK
                     RTPQQAWSERVVCVGTTAG"
     gene            2139..>6397
                     /locus_tag="PKK81_03115"
     CDS             2139..>6397
                     /locus_tag="PKK81_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV31890.1"
                     /translation="MLRNACQYQTLVELLRVRVSDAPSKHAYTYLRDGETDALTWDYG
                     ELDRRARAIAAWLQSCGAAGERALLLYPPGLDYLAAFFGCLYAGVVAVPAYPPQRQRG
                     WPRVRAMLADSQAAYALTTSGIARVIDRLSVKEDGYGSLATLQWLNTDNVPSGVDEDW
                     SEPDITAETLAFLQYTSGSTGLPKGVMVSHANLLHNQRLIQEAFGHTSQDVIVGWLPL
                     YHDMGLIGNVLQPLYLGASCILMSPVHFLQKPVRWLSAITRYRATTSGGPNFSYDLCV
                     RQITEAQRELLDLSSWTLAFNGAEPVRAATIERFSDRFRARGFRKEAFYPCYGLAEAT
                     LLVSGGTRGAGPLVQPFERSRLRSEPTASASGAASHLLVSCGVDRSGRQIRIVHPDSG
                     IACADGQEGEIWVGGPCVAQGYWQREAESRETFQASLAGTGEGPFLRTGDLGFLKDGE
                     LFVTGRLKDLIIIRGRNHYPHDIERTVEECHPALRPGAGAAFAITEGDEEQLVVVQEM
                     EPRSQALDLQAVTTAIRQTVAQAHEVSVFAVVLIKAGTLPKTSSGKVQRSLCRDKFLA
                     RELIAVEESVLRDSSAAVVVEEEPATVGDEDGLEQVLADIWSQVLKRPNVESGDDFFA
                     LGGDSLRGLQVVARVREVRGLDVPLDALFDHPTLAAFSAHVVTCVPADPGSPIESIRR
                     VSRDETLPLSFAQERFWFLDQLSSGSSINNIPVALRLVGALDVEALRRSLDEIIQRHE
                     ILRACFALRNGRPSHIIVPQLILALDIEDLRTSVEPDREAQILRLTGEEACRPFDLAN
                     GPLIRGCLFRLDDMNHVLSLTLHHIVADGWSMDVLRRELASLYEAFKDGRPSPLPALP
                     IQYVDLIAAQRERPKGAADRRLLEYWANQLEGAPPLLNLPWDYPRPAIQGHRGARHPW
                     TLEPELARRLYEVGARYRATPFMLLVSALGLLLSRYSRQTDFCIGTPVANRAGREAEQ
                     LVGCFVNTVVLRMDVSGNPSLPALLDRVRVMVLGAQSHQDLSFERLVDELGVVRDLGH
                     TPLFQVMFVLEDSPSDVRRFADLEASRLNVGTGTSVFDLTLEMAQKTDGSLDAVFEYS
                     TDLFAADTIARMAGHLQALLRQIVASPEASVSELSILSPGEQALELADSRSPREAYPQ
                     EASLHRLIEAQASKTPDAVALQCDRGPITYDSLNRLGNQLARYLRVQGIVPEAPVALC
                     VDRSLEMAIGLLGCLKAGATYVPVDPSYPAERQAAIFRDAQPHLLLTQGRHLESLPEH
                     TAPVLALDAGWPAVAGLPDDNLVTGPNPDHLAYLLYTSGTTGNPKGIGITHRALVNHG
                     LAMVQAYDLSPADRVLQFASVSFDVAAEECFPTWLAGATVVLRPNEPVPAYGDFQAFL
                     HAQGVTVLNLPTPYWAGWAEESERTAAAVPSSVRLVIVGSE"
     assembly_gap    6398..6497
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6498..7738
                     /locus_tag="PKK81_03120"
     CDS             <6498..7738
                     /locus_tag="PKK81_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV31891.1"
                     /translation="GDLYIGGPGLARGYHRQPALTAAQFIPHPFSHDRGARLYRTGDV
                     ARRRSDGNLEFLGRADEQVKIRGFRVEPQEIECHVRAHPEVKDARVFLESDAPGDWTA
                     VLAGLSDEEQDRLVRDVEALSAAEARWLYDLESDGEQRRKTVIQRKPEFEVYLKINKE
                     EFLTPPRANQRNWLLRRALDEFSDDLLALDEASKRFVSGSDRADIERGWAESRAEYNP
                     SELIIEGQQVMQDWEAPLMKAMADIVTDGHGDVLEVGFGMGISASYIQAGRPRSHTII
                     ECNRDVMRAFAEWTDKYPDRDIRLVQGRWQDVVEDIGLFDGVFFDTYPASEAEFEESV
                     INSINFAEGFIPVAAKLLRPGGVFTYYTNEIDSFSRRHQRFLLDHFGSFTLSIVRSLL
                     PPDDCHYWWADSMAVVKAVK"
     gene            7740..8384
                     /locus_tag="PKK81_03125"
     CDS             7740..8384
                     /locus_tag="PKK81_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNV31892.1"
                     /translation="MEERFQRIERLSDAQRRLLGLRAGRQARATEARLVGYIVPRTTG
                     GVTGQDLRGFLAQRLPDYMVPSSWVFLEAFPMTSRGKIDRKALRATGRSKPEALSAQG
                     TPRNDRERAVAAIWEEVLGVRGVGAHDNFFDLGGHSLLLPKLLTKVRAMTDREMSMVD
                     LFRYPTVHALAAALAGSHDTQADGIGSRQLKETRDAGARRMKQRRTKQIASRNE"
     gene            8381..12934
                     /locus_tag="PKK81_03130"
     CDS             8381..12934
                     /locus_tag="PKK81_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV31893.1"
                     /translation="MSHSPLEFDQTEPRDGLDIAIVGMAARFPGAPDLEAFWRNLCSG
                     VESVSRLTPQELAEAGVDRKTAGRQNYVGARAVLEGIDLFDAPFFSMTPKEAETTDPQ
                     QRLLLECAWETLEHAGYDPSRYKGSIGVYAGSSTSGYLYHLFPRRVLLQSPAEMSAML
                     GVEKDTLATRLSYKLNLRGPSLAVQTACSTSLVAVHLACQALLSGECDMALAGGVSVT
                     VPQKVGYLYQEGGIVSPDGHCRTFDAKAEGTVGGSGVGLVLLKRLQDALDEGDHILAV
                     VKGSAVNNDGAAKVGYTAPSIEGQAKVIEAAHVAAGVRADTISYIEAHGTGTPMGDPI
                     EVAALTQAFRASTSRTGFCAIASVKTNIGHLDAAAGIAGLIKTVLALVHRRIPPSLHY
                     AAPNPAIEFSTTPFYVQTALTEWAGGSSPRRAGVSSFGLGGTNAHVVIEEAPAATHRL
                     SPDDERWRLLILSARSNAALDRVSERLSEHLATPAKVNLDDVAFTLQCGRKAFAHRRW
                     VVAKTIEEARQALAHPFLMSGTAGPTGEARQVAFMLSGQGSQYPQMGRSLYDQEPTFR
                     RHVDQCASLLSRHLGRDIRQVLFPRSPGDAELIHRTALTQPALFVLEYALAQTWISWG
                     LSPVAMIGHSIGEYVAACLSGVFSLDDAMAIVAMRGRLMQALPAGGMAAVPLMEAEVV
                     PLLSAGLDLAAVNAPAQCVVSGSHDRLESFEAHLAARGIKMRRLVTSHAFHSSMMEPM
                     LARFESFLASLPSQEPQIPWVSNLTGTWIRREEATDPAYWSRHVRATVRFADGLRTLL
                     DDQPPVLLEVGPGRTLQALAQRHSGSTAVSSFASFSGREEDEMAGLLMTLGSLWGAGV
                     PVEWNGLQRERPPRRVPLPSYPFERQRYWLDPREDRPDSVADGGRRADLSQWFYELSW
                     KRSALVPTSSRRSPDHWLVFVGDRETEAVLRSQLESRVASVTTVFPGSGFERHADGSY
                     RVRPDSREDHAKLLEELTARGQVPHCLLHAWGLESTGTDTSADARMRAAQERGFLHLV
                     ILAQTFGARRDSRRSILVLAQGLHDVTGDELLRPEWTTMLGACLVIPQEYAGLSCRLI
                     DLDRTAFQPPFVNDWGERLVTEAGRSGGDRVVAYRGQHRWVRTFEPLCLDAKDGDRLP
                     FRQGGVCLITGGLGGVGLRVAEHLIRTVRARLVLVGRSQPTAEQREFVAALERLGSEV
                     LVCRADVADRAQMQRLIDEAHQRFGGIHAVVHSAGVAGGGLLALKTPNAVWEEFRAKV
                     QGAAVIDALFRNQPLELLLFCSSLTAVAGGVGQSAYCAANAFLDALAHAGRREARRVI
                     SVNFDRWRHLGMARQAEATLRALGAAEFDLDGMDAVEGQEVLRRIIAGPDLAQVVQSI
                     RDLPAVMASVPHLGQLDRGGLSQKDKSPDELIPASFGEVEKEGLEAQLAELWRQVLGL
                     EHVGLEGDFFQLGGESLSALQILNRIQELHHVELSLREFLSAPTIHGLAGQIRTAQAA
                     GRRKEQGIVPIPREGRRLRGVSA"
     gene            12970..14667
                     /locus_tag="PKK81_03135"
     CDS             12970..14667
                     /locus_tag="PKK81_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HNV31894.1"
                     /translation="MDLIRFLLRKSWRLVLCSVVAGLVSGLAGAGLIAVIHRGMNETQ
                     VSSTLAWSFLVLALAVPACKALSEILLTRLGQSTISELRFQLSRRILDAPLSQLEQLG
                     HHRLLAALSEDTDVIAQAYVQLPLIGINVATTIGCLAYLGWLSWPMLLLVGGFMAVGA
                     LTFQVQERKAIRLFTQSRETSDSLFRHFRSIMAGIKELKLHRERRHAFLTTLLHRTVK
                     AYERDFVGGMTVYSIASSWGLFLFYAAIGALLFLWPSWRPVSIDTVVGATLVLLYMMG
                     PFSQIVELLPGLGRANVALNKIDALGLSLAASTPSREADGRQAGTELDIRRWSRLDLI
                     GITHHHAPEGDERPFRIGPIDLTFRPGEVTFLVGGNGSGKTTLALLLLGLYQQESGSI
                     LLDGVPIDDANRDTYRQLFSTVFCDFHLFDSLLGLEQENLDGVARVYLERLQLESKVR
                     IERGLFSTQALSQGQRKRLALLTAYVEDRPFYLFDEWAADQDPLFRKVFYSELLPDLK
                     ARGKAVLVITHDDRYFSMADRCLRMDFGRLVECREQDGAAGANGGLSAPSRVAGAGK"
     gene            14701..16107
                     /locus_tag="PKK81_03140"
     CDS             14701..16107
                     /locus_tag="PKK81_03140"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HNV31895.1"
                     /translation="MTVHVDEMWQAIPTGCVPPRELSLANPYLERQAARESNARSYPR
                     RLPLALSKGKGIYVQDTDGRTYIDCLACAGALALGHNHPVVVEAINRMLRDGLPFQTL
                     DLTTPVKDGFVGMLFDCLPPAFADGAKIQFCGPSGADAVEAAIKLVKIARERRTILAF
                     HGAYHGMTHGTLGLTGHLAPKQALSGLMPDVHFLPFPYEYRCPFGVGGEKGSRLSAAY
                     IERLLDDPNSGVVEPAAMILEVVQGEGGVIPAAKSWLQEIRRMTDERGIPLIVDEVQT
                     GLGRTGKLFAFEHADIVPDVVVLSKAIGGGLPLSVVVYRPELDQWTAGAHAGTFRGNQ
                     LAMATGLATMEFITAERIDEHAARMGERLLACLRQIQGEARSLGDVRGRGLMIGVEIV
                     DRDAPIELPGCHPSAPRLASRIQHEALRRGLILELGGRHNCVVRFLPPLIVTAEQIDT
                     IATIFSEAVKAAEEQGVA"
     gene            16104..18491
                     /locus_tag="PKK81_03145"
     CDS             16104..18491
                     /locus_tag="PKK81_03145"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNV31896.1"
                     /translation="MISRGRVLAAVGVILGFGVVSLLFAGSASLPMRDGVLKLPGLQA
                     PVAVAFDAYGIPVVAAGDRLDAMRALGYVTAQDRLFQMDLLRRNSAGRLAEVLGESGL
                     QVDLRQRNLGLSPVAEAVLRRLPEEQRAVLEAYAEGVNAFLEQMTVPPFEFLLLGYRP
                     ARWSPTDSILLVLAMFQMLNGYEDDERMRTVMKASLPEEVAAFLLPPLDSYTARLFTG
                     NMTQRSPASVPVEALASLRQAGGQSRAVQTSVVPGRKRELGSNAWAVAGAKTEDGRAI
                     LANDMHLDVGVPNIWYRVQLRYGRVEVAGVIVPGIPVVIAGSNGLVSWGMTNVEGDFL
                     DLVRLDLNPNNADEYATPDGWRRFTIRNETIAVKGDRDRVVEIKETLWGPVAEKPLMG
                     HPVALRWIALDPDAVDLGLLQMDQARTVRDAIAVANRAGGPPNNVVLADADGHIAWTF
                     MGKIPVRRGFDGSVSVSWADGRTGWNGFIAPDELPRIVDPPSGYLVSANHRMVGEEYP
                     HVIGHAFANGYRAYRITERLRDKDRFHETDLLALQLDTTAQLYEFYRDLVKRLLNESV
                     IEGTPDLADVRRAVEAWEGRADSDSKGFGLLVAFRRTLAASVFAPFLQSCREQDAGFV
                     YDGDLDTPLRTLLTEQAPAVLPDPARFADWNTFLLQQLKQTVETLKTDYDLLSPDDLT
                     WGIMNRVRMTHPLSEAIPGLGRWLDMTDEEASGCGQCVRVLSGSLAASQRMVVSPSHH
                     SDAIFHMPGGQSGHPMSAHYRDQQRNWSEGVPTPLLAGRPTHNLTLQPSDRGGAS"
     gene            18526..18771
                     /locus_tag="PKK81_03150"
     CDS             18526..18771
                     /locus_tag="PKK81_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HNV31897.1"
                     /translation="MGQDEQEDTTVYKVVVNHEEQYSIWPSYRENPLGWQDAGKTGLK
                     DECLSYIKDVWTDMRPLSLRKWMAEQAEQADKTAAPC"
     gene            18765..19526
                     /locus_tag="PKK81_03155"
     CDS             18765..19526
                     /locus_tag="PKK81_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV31898.1"
                     /translation="MLTAADRRPWIVTCRRGDGLRLICFPYAGGGPSLFRGWPLELSQ
                     QVEVCAVQLPGREARMKEPPIGDLRRLVVELADAIEPSLDRPFALFGHSVGGLVAFEF
                     ARELRRRYGIEPVHLFVSGCPAPELTDDERLSELPDGEFLERMRRFNGTPREILEHAE
                     MMELVLPTLRADFSLRDTYRHRTESPLGCAISAFGGMADRAVGVDKLNPWSAHTTGGF
                     QLWLFQGDHFFVRTAQAAVVEAVMLLLQPYMSLRL"
     gene            19578..20612
                     /locus_tag="PKK81_03160"
     CDS             19578..20612
                     /locus_tag="PKK81_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HNV31899.1"
                     /translation="MNHESGSSCGTRPGVNGIVTRNGGLMIEPLSSDGSLPLVVTPVE
                     GRTLDGYLAAARHYAEAFLPVSGGLLFRGLPIGSVADFESFVGGFTSSLVSYEFGSTP
                     RSRVQRQVYTSTEYPPHQHIPLHNEQAYTREWPMKIWFYCGQAPEEGGYTPIADSREV
                     YRRIPACIREHFNRKQVMYMRNYGNGLDVPWQKVFNTEDPAVVEAFCRTNGILYEWKA
                     DGELRTRQVAQAIAVHPLTGETVWFNQAHLFHVSNLEPAVREDLLAVVAEEDLPRNAC
                     YGDGSPIESDLLDEIRDVYRSVAVQFSWQEGDVMMLDNMLAAHGRTPFKGRRQILVAM
                     AEPCRAGHPC"
     gene            20606..>25251
                     /locus_tag="PKK81_03165"
     CDS             20606..>25251
                     /locus_tag="PKK81_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNV31900.1"
                     /translation="MLMPHDQTQDRPVDVLGSEPEQYLFPMSFGQRRLWFLSQLEPES
                     ASYNTAIAVRMKGRVDRRLLVNSFNRIVERHEVLRTSFSTENGEPVQIIATVGRVTLE
                     TDLVTNQPEAILAAARDEAQRPFDLQRGPLLRLRLFDITDDDHLLVVTLHHIVCDGWS
                     AQILAREFAQLYVEAVEGTDAALPALPIQYADYAEWQRGWLQGEVLDRHLTYWTRQLA
                     GDLPVIELPTDRPRGAVQTSSGSRYVFTLPQALAGGLRELSRRHNVTLFMTLLAVFQT
                     WLMRYTGIDDVCVGTPIACRTKRETEDLIGFFANTLVLRTDLSQNPTFRQLLERVREV
                     VLGGQEHQELPFEELVDALKPARALSHSPLFQVLFALQTTLAEAVRLPGLHVEITEVD
                     AAAAKFDLSLDMAETEAGLEGAFEYNTDLFDAATVARMAGHFQTLLAGIVATPDCRLH
                     ELPLLSVEERQEALLVGPDDRVTDECFDFAAVFSAQVERSPGAVAVECRGATRTYAEL
                     NREADRIAGALTTSGIGPDKIVALLLDRGIGLLTAIVGVLKAGGTYLPLDPSHPPARW
                     GELIHSSAASVVLANESYRARVGEASPRFASGDAILLALEDVVGKPDNALPHPCTCSP
                     DQLAYVIYTSGSTGIPKGAMVTQRGLMNHLWSKRDSLGLGPNDVVAQTASACFDISVW
                     QHLAALICGGRTLIVPEDEARDPVQLLTCLERDAVTVAETVPALLKGMVDGPQAADVP
                     LRSLRWLLPTGEALPPQLCREWLRRYPHVPLVNAYGPAECADDVATATIASPPAADEV
                     SMPIGRPIQRMRLVVVDRWLAPVPVGVSGELCIGGVGVGRGYLQDPGRTAAAFVPDPF
                     GTEPGARLYRTGDLVRRRTDGSLEFLGRVDHQIKLRGVRIEPGEIDARLRELAGIRDA
                     VTVVREDRPGQKRLVAYVVTSEQAAPSVETWRATLRSQLPEVMIPSRFVRLDTLPRTP
                     NGKVDRASLPVPDDPGDGGYAAPRDSVEEQVALIWAEALQRERVGIHDNFFELGGDSI
                     LSLQVVSRARQAGFSLTPRQVFQHQTVAELAALLAQPSDVSPAITDEELSAELVDAAT
                     GAAAKAACPNHEDVYPLSPLQQGLLFHSLYAPASGVYIEQMSWRMAGELDVPLFREAW
                     QQIVDRHPLLRTRFLWREVSMPIQIVLPAAEIAWREVDARDCASNEQAARFSRLVEED
                     RLTDFDFERPPLMRILLFRVGRDTYDVLWTHHHILMDGWCLPILLQEVLTAYQALRRG
                     AAPLFEPVAPYRHHIVRLLGQDQAEAQRFWRKALKGVTTPTVLGEEVAPEEVGQHRSS
                     GTAAITLSPETSAQLRTFAQGHHVTLNTLVQGAWALLLSRYSRESEVLFGTTVSGRSA
                     DIPGIERMVGLFINTLPLRVRVAPDAVLSEWLRALLERNSELRQFEQTPLVQIQSWSD
                     VPRGQAFFESLIVFDNHPLDESLEKATGLTIQGVTLQGQTNYPLTLNVLPGKELTLSL
                     WYHRNRFRDETAARMVRQLETLLRAMIQNPHARLGLLPLLASAERAQVVGAWNRT"
BASE COUNT         4442 a         7336 c         8310 g         5063 t
ORIGIN      
        1 cctgtgccgg tttagcaaac cggtgccttc ggccactcgg ccacctctcc gcaagtcgct
       61 acccgcagaa tttgtattga gttcccgcgc ctagatcctt cgaatacgaa ccctctcgaa
      121 gaagaggtgg gatcataccc cagagcatgg agcgcactca agtaattctc gcgaatgatt
      181 ttgacgccgg agcggccggc ttttagcgga gtgaggtgga cgtggggacg tgccgcgatc
      241 ttcaactatc tttttccaaa ttccttcagg atatcgagaa aagtggtctg ccactgggcg
      301 agcaggccca tgtgtcgttt gtggtctgtg gtgtgaacgc gctttccgta cactgatctc
      361 cccggagaca aggttcgaga cggactcatc tgtgcgtgtg aagttcccat gcccggggga
      421 tcgatctcgg cggatcggat caccccgctg gtcagaagca ttcgcagcgc atgcgctttc
      481 tgttgggatg atttgcgtat cacctttttc gaactggcca tgaatcctcg tggtgcagtg
      541 tgtgtgggtc gtgacaccgg gtggacgaag atcgctacat ccacagaata cggcccagat
      601 cttcgtcgag ttccttggct cgttggcatt ggctgcagcg ggcgaagagg ccgtcggtgc
      661 cgggtgaggt ctgagaacgg agtaccaatt gatacttgtg tcctccgcag aacgggcagg
      721 ccttgccatg aagttctcgg cgcacggatc cgattcttcc gacttgttta tgcatagtgc
      781 ctccggtgaa aggggagagt catttggggg agcctgccgg gggattgggg cagggtttgt
      841 aatgactcgt gcatgctcaa caatacccag caagcgggat gctgagaata ccgtggagga
      901 ggggaggggt tgtgatctaa ggaggggggt gcggtgaagc ggaaatattg tctttgtgca
      961 tgtagatagt aatctttaaa tatcaaatat atatgcgatg tacatgatgt gatttgggag
     1021 atattgccag gagcaggaag ctgaagataa gggatggtcc tgggagcgcc gggagggcaa
     1081 agagaataga agataaattc gggggtgaag gcagatcccg ccaggaggat gtcgagacct
     1141 cttgccgccc tgggtgggca caaccaaagc agacaccttc accctttttc agtcaaccat
     1201 gccgtcaggt ggtcgtgcaa gtccgtagtt gttcggaggt ggagatatgg ggggagatgg
     1261 gaatgcacct ccggggcgca ttcccatcgt ggagaggagc ctcttgatgc gggacggtta
     1321 gagtggagac catgcccatg gcggatgctg atcgggctcc aaccggctat cccgcggtgg
     1381 tgcccacgca gacaacccgc tcactccaag cctgttgagg cgtccgtttc tcgatatggc
     1441 cggagaggaa acggagccgg gtctggtcgg tctcggacat ccggataaat tcgagcccga
     1501 cctgcccgtt cttcgcccat cgaacagccg ccaatttgac ttgcagggcc gtcgcactgg
     1561 tcgggaagga taggcgcagg tccacatact gccccacctt gagtttcata ctggttttca
     1621 gcaggcaacc cggcacggag agattcagca gctgtccctg ccccatcatt ccttcacagg
     1681 agaggataca agtgctctgg accggttcgc gcttggtgta tcgctgtttc acaatgcctc
     1741 ctggttaggt gagcccctca tgtctcgtta tggtccaagt atatgccggg atcgaacgat
     1801 gtcattccgc gagaggtgtc cctcgaatgg ggggctgccg tggattgtga gcaactcatg
     1861 acgactgcac tcgcagccgc gtgatgattc aaccggcggc tgcagaccga ttttccaacc
     1921 tcctgcactg gattattcgc ggaccctccc catctttccg tcatcgtcca taggcccttc
     1981 atcttcgacg tccagcacga attgtttcca tcgatggggc ggatctggtg atcgagcagc
     2041 aggatccgga aagtgaacat gcaagatttt cgatgagggg cgtcttgtag agtagacaca
     2101 cattcacgca acggtcggga aggcagggtg gattcgagat gctgcgcaac gcttgtcaat
     2161 accagacact cgtcgagctc cttcgtgtgc gggtgtcgga cgccccatct aaacatgcgt
     2221 atacgtatct tcgcgacggt gagacggatg cgctgacgtg ggactatggc gagttggatc
     2281 gccgcgccag ggccatcgcc gcgtggttgc agtcgtgtgg cgcggcagga gagcgggcgc
     2341 tgttgctgta cccgccgggg ctcgactatc tggccgcgtt tttcggttgt ctctatgccg
     2401 gagtggtggc cgtgccggcc tatccgccgc agcggcaacg cggctggccg agagttcgag
     2461 cgatgcttgc agattcgcaa gcggcctacg cattaacgac gagcggcatc gcgcgggtga
     2521 tcgaccggct ctccgtcaag gaggacgggt acgggagtct ggcgacgctc cagtggctca
     2581 ataccgacaa cgtgccgtcg ggtgtcgacg aagattggag tgagccggac attacagcgg
     2641 agacgctggc ttttctgcaa tacacctccg gctcaaccgg ccttccgaag ggcgtgatgg
     2701 tcagtcatgc caatctgttg cataaccagc gcttgattca ggaggccttc ggccatacgt
     2761 cgcaagacgt catcgtcggg tggctgccgc tgtatcacga catggggttg atcggcaatg
     2821 tgttgcaacc cctctacctc ggcgcctcct gcatcttgat gtctccggtg cattttcttc
     2881 agaagccggt acggtggctg tcggcgatca cgcggtaccg cgcgacgacc agcggggggc
     2941 cgaatttctc gtatgacctc tgtgtgcggc agattaccga ggcgcagcgg gaattgctgg
     3001 atttgagttc gtggaccctg gccttcaacg gtgccgaacc cgttcgcgcc gcgacgatcg
     3061 aacggttctc cgacaggttt cgagcccgtg gattccgcaa ggaggctttt tatccctgtt
     3121 atggattggc cgaggcgacg ctgctggtct cgggtggaac ccgtggagcg ggacccctcg
     3181 tgcagccgtt cgagcgctcc cgactccgat ctgagccgac ggcgtctgca tccggcgcgg
     3241 caagccatct cctggtgagc tgcggtgtcg accgatcagg gcgtcagatc cgcatcgtcc
     3301 atcccgactc cggaatcgcc tgtgcggatg gtcaggaggg ggagatttgg gtgggtggac
     3361 cttgcgtcgc tcagggttat tggcagaggg aagcagaatc ccgggagacc tttcaggcat
     3421 cacttgccgg gacaggagag gggcccttcc tccggaccgg agatctcggg ttcctcaaag
     3481 acggggaact gttcgtcacc ggcagattga aagacctcat catcatccgg ggccgcaatc
     3541 actatccgca cgacatcgaa cggacagtcg aggagtgcca tccggcgctc aggcctggag
     3601 ccggagcggc attcgcgatc actgaagggg atgaagagca acttgtggtg gtgcaggaga
     3661 tggagccgcg ctcccaggcc ctcgatctgc aagcggtcac cactgccatc cgacagacgg
     3721 tggcgcaggc gcatgaggtg tcggtttttg cagtggtgct catcaaggca gggacgcttc
     3781 caaaaacctc gagcggaaag gttcagcgaa gtctgtgccg tgacaaattt ctcgcccggg
     3841 aattgatcgc ggtcgaagag tcggtgctga gggattcctc cgcagcagtc gttgtggagg
     3901 aggagcctgc aacagtcggg gacgaggacg gcctcgagca ggtgctggcg gacatctggt
     3961 cgcaggtgtt gaagcggccc aacgtcgagt cgggcgacga cttcttcgcc ctgggcggcg
     4021 actccttgcg cgggcttcag gtggtggcgc gggtgcggga ggtccgcgga ctcgatgttc
     4081 ccttggacgc gctgttcgat cacccgacgc ttgccgcttt ctcggctcat gtcgtgacct
     4141 gtgtgccggc cgatccaggt tccccgatcg aatcgatccg gcgggtgtca cgcgacgaaa
     4201 cactgccgct gtctttcgcg caagaacggt tttggtttct ggaccagttg tcttcaggca
     4261 gttcgatcaa caacattccg gtcgccttgc ggcttgtcgg tgcccttgac gtggaggcgt
     4321 tacgccggtc gctggatgag atcattcagc gccacgagat ccttcgcgca tgtttcgcgt
     4381 tgcgcaacgg ccgtccctca cacatcatcg tgccccaact tatcctcgct ctggatatcg
     4441 aagacctgcg cacatcggtt gagcctgaca gggaggcgca gatcctgcgc ttgaccggag
     4501 aagaagcctg tcgcccgttc gaccttgcca atgggccgct cattcgtggc tgcctcttcc
     4561 ggctcgacga tatgaatcac gtgctctcgc tgaccttgca tcacatcgtg gcggacggtt
     4621 ggtcgatgga tgtgctcagg cgggaattgg cctccttgta tgaggccttc aaggatggtc
     4681 gcccgtcacc cctgccggct ctcccgattc aatatgtcga tctgatcgcc gctcagcgag
     4741 agagacccaa gggcgcagct gatcgaaggt tgcttgaata ttgggcgaat caattggagg
     4801 gggcgcctcc gttgctgaac ctgccctggg actatccgcg accggccatc caaggtcatc
     4861 ggggcgcccg tcatccgtgg acgctggaac cggagctcgc gcgtcgcctg tatgaagtgg
     4921 gggcccgcta ccgcgccacg ccgttcatgt tgttggtctc tgccctcggt ctcctcttgt
     4981 ctcggtatag ccgccagacc gatttctgca tcggcacccc ggtggccaac cgcgcgggac
     5041 gcgaagcgga acaactggtg ggctgttttg tcaacaccgt cgtgctccgg atggatgtgt
     5101 ccggcaatcc ctcgttgccg gctctgctgg atcgcgttcg cgtgatggtg ctcggggcgc
     5161 agagccatca ggacctgtcc tttgagcgtt tggtcgacga actgggtgtg gtgcgggatc
     5221 tcggccacac gccgctcttt caggtgatgt tcgtgctgga ggatagcccc tcggacgtcc
     5281 gtcggttcgc ggaccttgag gcttcccggc tgaacgtcgg cacggggacc tccgtcttcg
     5341 atctcacgct ggagatggca cagaagaccg atggaagcct ggatgcggtc ttcgaataca
     5401 gtacggatct ctttgccgcg gacacaatcg cgcggatggc cgggcatctg caagcactgc
     5461 tgcgacagat cgtcgcctcg ccggaggcat cggtgagtga gttgtcgatc ctctcgcccg
     5521 gcgagcaggc cttggagctt gccgatagtc gcagtccacg ggaggcctat ccgcaggaag
     5581 cctcgctcca tcgcttgata gaagcgcagg cgagcaagac accggatgcc gtggcgctgc
     5641 aatgcgaccg aggccccatc acctatgatt cgttgaaccg cctgggcaac caactcgccc
     5701 gttatctgcg agtgcagggg atcgtccctg aggcgcctgt cgcgctttgc gtggaccgat
     5761 cgctggagat ggccatcggc ctcctgggct gtctcaaggc cggcgcgacc tatgtaccgg
     5821 tcgatccctc ctatcccgct gaacggcagg cggccatttt cagggatgcg cagcctcacc
     5881 tcttgttgac tcagggaaga catctcgaaa gccttcccga gcacacagcg cctgtcttgg
     5941 cgcttgatgc cggatggccg gctgtcgccg gtttgcctga cgataatctt gtcaccggcc
     6001 cgaatccgga ccaccttgcg tatttgctct atacgtccgg caccacaggg aatcccaagg
     6061 gcatcgggat tactcatcgg gccttggtga accacggcct tgcgatggtt caggcctatg
     6121 acctgtcccc ggcggatcgc gtgttgcagt ttgcctctgt gagttttgac gtcgcggcag
     6181 aagaatgttt ccccacctgg ctggccgggg cgacagtcgt cctgcgccct aatgagccgg
     6241 ttccagccta tggcgatttc caggctttct tacatgccca gggggtgacg gtgctgaacc
     6301 tgccgactcc ctattgggcc ggctgggccg aggagagtga gcgcacggct gcggccgttc
     6361 catcgtcggt cagactggtc atcgtaggaa gcgaaaannn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnccg gcgacctgta catcggcggt ccaggccttg cgcgaggcta
     6541 tcatcggcaa ccggcgctga cagccgcaca atttattccc catcctttta gtcatgacag
     6601 gggggcgcgc ctgtatcgca ccggcgatgt ggcccgccgc cgttcggacg gaaatcttga
     6661 atttctcggg cgtgccgatg agcaggtcaa gattcgtggg ttccgagtag aaccgcaaga
     6721 gatcgaatgc cacgtgcgcg cccatcccga ggtcaaagac gcgcgggtat ttctggaatc
     6781 cgacgctccc ggcgattgga ccgcggtcct ggccggtctc agtgacgagg agcaagaccg
     6841 gctggtgcgg gacgtggaag ccctgtcggc tgcggaagcg cgctggttgt acgacctgga
     6901 gtcggatgga gaacaacgga ggaagacggt gattcaacgg aagccggagt tcgaggtgta
     6961 tctcaagatc aacaaggaag aatttttgac gcctccacgc gccaaccaac ggaattggct
     7021 gctgcgccgc gccctcgatg agttctctga tgatcttttg gccctcgacg aggcgtcgaa
     7081 acgcttcgtc tcaggatcgg atcgggccga catcgaacgc ggctgggccg agagccgggc
     7141 ggagtacaac ccgtcggaac tcatcatcga agggcagcag gtcatgcagg attgggaagc
     7201 gccgctgatg aaggccatgg ccgacatcgt gaccgacggg cacggcgatg tgcttgaggt
     7261 ggggttcggt atggggatct cggcgtcgta cattcaggcg ggccggcccc gctcgcatac
     7321 catcatcgag tgtaaccgtg atgtcatgcg cgccttcgcg gagtggacgg acaagtaccc
     7381 ggatcgcgac atcaggctcg tgcagggacg gtggcaggat gtcgtggagg acatcggcct
     7441 gttcgacggg gtgttcttcg atacctaccc ggctagcgag gccgaatttg aagagtccgt
     7501 catcaacagc atcaattttg ccgaagggtt cattccggtg gccgccaagc ttctgcgtcc
     7561 gggcggcgtc ttcacgtact acaccaacga gatcgattcg ttcagccggc gacatcaacg
     7621 cttcctgctc gatcatttcg ggtcgttcac cctcagcatc gtccggtcgc ttctccctcc
     7681 ggacgactgc cactactggt gggccgactc catggcggtc gtcaaggcgg tgaagtgaca
     7741 tggaggagcg gttccagcgg atcgaacggt tgagcgatgc ccagcgccga ttgctggggc
     7801 tgcgcgcggg acgacaggcg cgggcgaccg aagcccggtt ggtggggtac atcgttcccc
     7861 gcacgaccgg cggggttacg ggacaggacc ttcgcggctt tctcgcgcaa cggctcccgg
     7921 actacatggt gccgagcagc tgggtcttcc tggaggcctt tcccatgact tctcgtggaa
     7981 agatcgaccg gaaggcgttg cgggcgacgg ggcgatccaa accagaagct ttgtcggcgc
     8041 agggaacgcc gcgcaacgac cgcgaacggg cggtggcggc gatatgggaa gaggtcctgg
     8101 gggttcgcgg cgtcggtgcc cacgacaatt ttttcgacct cggagggcat tccctgctgt
     8161 tgcccaagct gttgacgaag gtgcgggcaa tgaccgaccg ggagatgtcg atggtggacc
     8221 tatttcggta tcccactgtc catgcgctgg ccgctgctct cgccggatct cacgatacac
     8281 aagccgatgg aatcggcagc cgtcaattga aagagacacg cgacgcggga gcgcgtcgga
     8341 tgaagcagcg gcgaaccaag cagatcgcat cgaggaacga atgagccatt ctcccctgga
     8401 attcgaccag accgagccgc gcgatgggtt ggacatcgcc atcgtgggca tggctgcgcg
     8461 ctttcccggt gctccagacc ttgaagcttt ctggcgcaat ctctgctcgg gggtggagtc
     8521 ggtgtcgcgg ctgactccgc aggaattggc cgaggcgggc gtcgaccgga agacggccgg
     8581 gcgtcaaaat tacgtagggg cgcgcgccgt gctggagggg atcgatctct tcgatgcgcc
     8641 ttttttcagt atgacgccga aagaagcgga gacgacggat ccgcagcaac gtctcttgct
     8701 ggaatgcgca tgggagacgt tggaacatgc cggctacgat ccgtcccgct acaaggggtc
     8761 gatcggggtg tatgccggtt cgagcacgag cggctatctc tatcacctgt ttccgcgccg
     8821 ggtgttgttg cagtcgcccg cagagatgtc ggcgatgctc ggggtggaaa aagacacgct
     8881 ggcgaccaga ctctcctaca aattgaattt gcgcgggccg agcctggctg tacagacggc
     8941 ttgctccaca tcgcttgtgg ccgtgcacct ggcctgtcag gccttgctga gcggcgaatg
     9001 cgatatggcg ctggcgggcg gcgtgtccgt gacggtgccg caaaaagtcg gctacctcta
     9061 tcaggaaggc ggcatcgttt ccccggacgg gcactgccgc accttcgatg caaaagcgga
     9121 aggcaccgtc ggcggcagcg gggtggggct cgtccttctc aagcggctgc aggatgcgct
     9181 ggatgagggc gatcacatcc tggccgtcgt caaggggtcc gccgtcaata acgatggggc
     9241 tgcgaaggtg ggttataccg cacccagcat cgagggccag gcgaaggtga tcgaggccgc
     9301 tcacgtggcg gccggcgttc gggcggatac gatttcctac atcgaggctc acgggaccgg
     9361 cacgccgatg ggagatccca tcgaagtggc cgccttgacc caggcctttc gtgcgtccac
     9421 ctccaggacg gggttttgtg ccatcgcgtc ggtgaagacc aatatcggtc atctcgatgc
     9481 ggcggcgggt atcgccggtc tgatcaagac ggtgctcgca ctcgtccatc gacgcattcc
     9541 cccgagcctg cactacgcgg cgcccaatcc cgccatcgag ttttcgacga cgccttttta
     9601 tgtgcagacg gcgttgacgg agtgggccgg cggctcgtct ccgcgccggg ccggcgtgag
     9661 ttcgttcggc ctcgggggga cgaatgccca tgtggtcatc gaggaagctc ccgcagcgac
     9721 acatcgcctg tctcccgatg atgaacggtg gcgcctgttg atcctgtcgg cccgttcgaa
     9781 cgccgccctc gaccgggttt cggagcgttt gtccgaacat cttgccacgc cggccaaagt
     9841 taatctcgac gacgtggcct tcacgctgca gtgcgggcgc aaggcctttg cgcatcggcg
     9901 gtgggtggta gcgaaaacga tcgaggaggc tcgacaggct ctggcacatc cgtttctgat
     9961 gtccggcacg gccgggccga ccggcgaagc ccgtcaggtg gccttcatgt tgtccggcca
    10021 aggctcgcaa tatccgcaga tgggccggtc gctctacgac caggagccga cattccgtcg
    10081 gcatgtcgat cagtgcgcct ctctcctgtc ccggcacctc ggacgggata tcaggcaggt
    10141 gttgtttccc cgttcgccgg gggatgcgga attgatccat cgaacggcgc tgacccagcc
    10201 ggccttgttc gtgttggaat atgccctcgc gcagacctgg atatcctggg ggctctcccc
    10261 cgtcgcaatg atcggtcaca gcatcgggga gtatgtcgcc gcctgcctgt cgggtgtctt
    10321 ttcattggac gatgcgatgg cgatcgtcgc catgcggggg cggttgatgc aagccctgcc
    10381 cgcgggagga atggcagctg ttcctctgat ggaggcggaa gtagttcctc tgctgtccgc
    10441 cggactcgat ctcgcggcgg tgaatgctcc agcccaatgc gtcgtgtccg ggtcccacga
    10501 ccggctcgaa tcctttgagg cccacttggc cgcccggggc atcaagatgc gtcgcctcgt
    10561 gacgtcccat gccttccatt cctccatgat ggaacccatg ctcgcacgtt tcgaatcctt
    10621 tctggcgagc ctgccatcgc aggagccgca gattccctgg gtctcgaatc tgaccggcac
    10681 ctggatccga cgcgaggagg ccaccgatcc cgcctattgg agccgtcacg ttcgtgccac
    10741 cgttcggttc gccgatggac tgcgcacgtt gctcgatgac cagcccccgg tgctcttgga
    10801 agtcgggccg gggcggacgt tacaggcctt ggcccagcgc cactccggtt cgacggctgt
    10861 ttcctcgttc gcctccttca gtggaaggga ggaggacgaa atggccggcc tgttgatgac
    10921 gttaggatcg ctctggggtg ccggagtgcc ggttgagtgg aacgggctgc aacgcgagcg
    10981 tcctcccaga cgggtgccct tgccctccta tccgttcgag cggcagcgct actggctcga
    11041 ccctcgtgag gaccggcccg attccgtcgc ggacggcggg cgtcgcgccg atctctcgca
    11101 atggttctat gaactgtcct ggaaacggtc ggcgctcgtc ccgacatcgt cccgtcgaag
    11161 ccctgaccat tggttggtct ttgtcggcga tcgagagact gaagccgtgc ttcgttccca
    11221 actcgagtcg cgcgtggcct ccgtgaccac tgtattccca ggaagcggct tcgaacgtca
    11281 cgcggatggg agttatcgag tgcgtccgga ctctcgtgag gatcatgcaa agctgctcga
    11341 agagctgacg gccaggggcc aggttccaca ctgcctcctg catgcctggg ggctcgaatc
    11401 gacggggaca gacacgtctg ccgatgcgcg gatgcgtgcg gctcaggaac gagggttcct
    11461 ccaccttgtg atcctggcac aaacgtttgg agcgcggcgc gacagccggc gttcgatcct
    11521 ggtgctggcc cagggactcc atgatgtgac cggcgatgag ttgttgcgtc cggaatggac
    11581 caccatgctg ggcgcttgtc tggtgatacc gcaggaatac gccgggctct cctgccgcct
    11641 gatcgatctc gaccggacgg catttcagcc tccgttcgtg aatgactggg gtgagcggct
    11701 ggtgacggaa gccgggcgtt ccggcggcga tcgcgtggtg gcgtaccggg gacagcatcg
    11761 gtgggtgcga accttcgagc cgctttgcct tgatgcgaag gatggagacc ggttgccgtt
    11821 tcgtcagggt ggtgtctgcc tgatcaccgg cggcttgggc ggagtcggcc tccgtgtggc
    11881 ggaacatctc atacgaaccg tccgggcgcg attggtcctg gtggggcgat cgcaaccgac
    11941 ggcggaacag cgcgagttcg tggcagctct ggagcgtctc ggcagcgagg tgctggtgtg
    12001 ccgggcagac gtggctgatc gcgcgcagat gcagaggctg atcgacgagg cacatcaacg
    12061 gttcggtggg atccatgcgg tggttcattc ggccggagtg gccggcggcg gactcctggc
    12121 cctgaagacg ccgaacgcgg tgtgggaaga gtttcgagcg aaggtgcagg gggcggctgt
    12181 catcgacgcg ttgttccgca atcaaccgtt ggagttgttg ctgttctgtt cctcactgac
    12241 ggcggtggcc gggggagtcg gccaaagtgc ctattgtgcg gcgaatgcct ttctggatgc
    12301 ccttgcccat gcaggacgga gagaagcgcg tcgggtcatc tccgtgaatt tcgatcgctg
    12361 gcgtcacctc ggcatggcca gacaggccga ggccacgctc cgggcattgg gcgcggctga
    12421 attcgatttg gatggaatgg atgcggtgga agggcaggag gtcctccgcc gcatcatcgc
    12481 ggggccggat ctcgcacagg tggtgcaatc gattcgagac ttgcctgcgg tgatggcctc
    12541 cgtcccacac ctcggtcaac tcgatcgcgg gggcttgtca cagaaggaca agtctcccga
    12601 cgaactgatc ccagcctcct tcggcgaggt ggagaaagag ggactggagg cgcaactcgc
    12661 cgaactgtgg cgacaggtct tagggctgga gcacgtgggc cttgagggag atttctttca
    12721 actggggggc gaatccctga gtgccctgca aatcttaaac cgtattcagg aactgcacca
    12781 cgtggagctg tcgctgcggg aattcctctc cgcgcccacc atccacggac tggcaggaca
    12841 gatccggacg gcacaggcgg cagggcgacg gaaagaacaa ggcattgttc caatcccgcg
    12901 ggaaggcaga cgtctccgcg gcgtctcggc ctaggtgagg cccgagtcct tggaaagaac
    12961 agtacgacga tggatctgat tcgattcttg ttacgaaaat cctggcggct cgtgctctgt
    13021 tcggtcgttg ccggactggt gagcggtttg gccggagcag gcctcatcgc cgtcatccat
    13081 cgtgggatga acgagacaca ggtgtcgtcc acgctggcgt ggagttttct ggtcttggct
    13141 ctggcggtcc ccgcctgcaa agccctgtcg gagattctcc tgaccaggct cgggcagtcg
    13201 accatttccg aactccggtt tcagttgagc cggcgcatcc tcgatgcgcc gctgagtcag
    13261 ctcgaacaac tgggccatca tcgcttgctg gctgcgctga gcgaggacac cgacgtgatc
    13321 gcgcaggcat atgtgcagct cccgctcatc ggcatcaatg tggcgaccac gatcgggtgt
    13381 ctggcttacc tgggctggtt atcctggccg atgctgctgc tcgtgggggg attcatggcg
    13441 gtcggtgccc tcacctttca ggtacaggag cggaaggcca tccggctctt cacacaatcc
    13501 cgggagacca gtgactcgct gttccggcat ttccgttcga tcatggcagg gatcaaggag
    13561 ctcaagctgc accgcgagcg gcggcatgcg ttcctcacca cgctactcca tcgaacggtg
    13621 aaggcgtacg aacgggattt cgtcggcggc atgacggtgt actccatcgc ttccagctgg
    13681 ggcctgtttc tgttttacgc cgccatcggg gcgctgttgt tcctgtggcc gtcgtggcgg
    13741 ccggtgtcca tcgatacggt ggtcggcgcc acgctggtgc tgctgtacat gatgggtcct
    13801 ttttctcaga tcgtcgagct gctgcccggt ctcgggcgtg ccaatgtggc gttgaacaaa
    13861 atcgatgcgc tgggcttgtc cctcgcggca tcgacaccga gtcgcgaagc agatgggcga
    13921 caggccggga ccgagctgga tatccgtcgg tggagccggc tcgacctgat cggcattacg
    13981 catcaccatg cgcccgaagg agatgagagg ccgttccgaa ttggtcccat cgatctgact
    14041 ttccgccccg gcgaggtgac ttttctggtc ggcggcaatg ggagcggcaa gaccacattg
    14101 gccctgttgc tgctggggct ctatcagcag gaatccggca gcattctcct ggacggtgtg
    14161 ccgatcgacg acgccaatcg tgacacctat cggcaactct tctccacggt cttctgcgat
    14221 tttcatctct tcgattcgct gctcggcctg gaacaggaga atctggacgg ggtggcgcgc
    14281 gtctatctgg agcgattgca gctcgagtcc aaagtcagga tcgaaagagg actgttttcg
    14341 acgcaggccc tctcgcaggg acagcgtaaa cggctcgccc tgctgacggc ctacgtggaa
    14401 gaccgtccat tctatctgtt cgacgagtgg gcagccgacc aggatcctct cttccgcaag
    14461 gtgttttact cggagttgtt gcccgacttg aaagcgcggg gaaaagcggt gttggtgatc
    14521 acgcatgacg accggtattt ttcgatggcc gatcgctgtc ttcgcatgga cttcggccgg
    14581 ctggtcgagt gtcgcgaaca ggacggggcg gcgggagcga acggcggcct gtccgcgccg
    14641 tcgcgagttg caggagcggg caagtgagag gacgtgtgtc tgtcacgagg aggagatctg
    14701 atgacggttc atgtcgatga aatgtggcaa gccattccga ccggatgtgt tcccccgaga
    14761 gagctgtcgc ttgcgaatcc ctatctggag aggcaggccg ctcgggagtc gaatgcccgg
    14821 agttatccgc ggcgtttgcc tctggcgttg agcaagggca agggcatcta cgttcaggat
    14881 accgacgggc gtacctatat cgattgtctg gcctgcgccg gcgcattggc gctcgggcat
    14941 aaccatccgg tcgtcgtcga ggcgatcaac cggatgttgc gggacggcct tccgtttcaa
    15001 accctggatt tgacgacgcc ggtcaaggac ggattcgtgg gcatgctgtt cgactgtctg
    15061 ccgcccgcgt ttgccgatgg cgcgaagatc caattttgcg gaccctccgg ggcggatgcg
    15121 gtggaggcgg cgatcaagct ggtgaaaatc gcccgtgaac gccgcacgat tctggccttt
    15181 cacggcgcct accacggcat gacgcacgga acgctgggac tcaccggtca tctggcgccg
    15241 aagcaggcct tgagcgggct catgcccgac gtacacttcc tgccgtttcc ttatgagtat
    15301 cgctgcccct tcggcgtcgg gggagaaaag ggcagtcgcc tgtccgccgc ctacatcgaa
    15361 cggttgctcg atgatccgaa cagcggcgtg gtggaaccgg ctgccatgat tctcgaggtg
    15421 gtgcagggag aagggggcgt gataccggcg gccaagtcct ggctgcagga gatccggcga
    15481 atgaccgacg agcgcggcat tccgctgatc gtcgatgagg tccagaccgg gctcggccgg
    15541 accgggaagt tgtttgcgtt cgaacatgcg gacatcgtgc cggacgtcgt ggtgttgtcg
    15601 aaagccatcg gcggcgggct gccgctcagc gtggtggtct accgtcccga gttggaccag
    15661 tggacggcgg gagcgcatgc cggaacattc aggggcaacc agttggcgat ggcgacgggc
    15721 ctggccacca tggagttcat caccgcagag cgcatcgacg aacatgcggc ccggatgggt
    15781 gagcggctac ttgcctgtct tcggcagatc cagggggaag cccggagcct gggggatgtg
    15841 cggggacggg gcctcatgat cggcgtcgag atcgtggatc gggatgcgcc gatcgaactg
    15901 ccgggctgcc atccctcggc gccgcgactc gcgtccagga ttcagcacga ggccctgcgt
    15961 cgcggtctga tcctggaact cggcgggcgc cacaactgcg tcgttcgctt tctgccgccg
    16021 ctgatcgtca cggcggaaca gatcgatacg atcgcgacca ttttttcgga agcggtcaaa
    16081 gcagcggagg aacagggcgt cgcatgatca gcagaggacg tgtcctcgca gccgtcgggg
    16141 tcattctggg gttcggggtt gtctccctcc tgtttgcagg ttccgcgtcc ctgccgatga
    16201 gagacggcgt cctaaagctg ccggggttgc aggcgccggt ggccgttgcc ttcgatgcgt
    16261 atggaatccc ggtcgtggcg gccggggatc ggctcgatgc catgcgagca ctcgggtatg
    16321 tgacggccca ggaccggctc tttcaaatgg acctgctccg gcgaaacagc gcgggacgac
    16381 tggccgaagt gttgggggag tccggactgc aggtcgatct caggcagcgg aatttgggct
    16441 tgtcgccggt cgcagaggcg gtgctcagac ggttgccgga ggaacaacga gccgtcctgg
    16501 aggcttatgc ggaaggcgtg aatgcgtttc tggaacagat gacggttcct ccttttgaat
    16561 ttctgttgct ggggtatcgg cccgctcgat ggagcccgac ggacagcatc ctgctggtgt
    16621 tggccatgtt tcagatgttg aacggatatg aggacgacga acggatgcgc acggtaatga
    16681 aggcttcgct tcccgaggaa gtcgcggcct tcctcctgcc gccgctcgat tcctacaccg
    16741 cacggctgtt tacggggaac atgacacaac ggtcgcctgc ctccgtgccg gtcgaagctt
    16801 tggcgtcatt gcgtcaagcg ggcggacaaa gccgtgccgt tcagacctca gtcgtaccag
    16861 gccggaaacg ggagctgggc tcgaatgcct gggctgtggc cggcgcgaaa accgaagacg
    16921 gtcgcgccat tctggccaac gatatgcacc tcgacgtcgg ggtgccgaac atctggtatc
    16981 gggttcagtt gagatatgga cgggtcgaag tggccggcgt gatcgtgccg gggattcccg
    17041 tggtgatcgc cgggtccaat ggcctggtct cctggggcat gaccaatgtc gagggcgatt
    17101 ttctcgacct tgtgcgcctc gatctcaatc cgaacaatgc ggacgagtat gcgacccctg
    17161 acggctggcg tcggttcacg atccgcaacg aaaccattgc cgtcaagggc gatcgagacc
    17221 gggtggtgga aatcaaagag acgctctggg ggcccgtggc cgagaagcca ttgatgggac
    17281 atcccgtcgc gctacgttgg atcgccctcg atcctgatgc ggtggacctc ggcctcctgc
    17341 agatggatca ggctcgcacg gtgagggatg cgatcgcggt cgccaatcgc gccggcgggc
    17401 ctccgaacaa tgtggtgctc gcggacgccg atgggcacat tgcctggacg ttcatgggaa
    17461 aaattccggt caggcgcgga ttcgacggat ccgtcagtgt gtcctgggcc gatggacgga
    17521 cgggctggaa tggattcatc gcgccggacg aactgccgcg gatcgtcgat ccgccatcgg
    17581 gttacctcgt gagtgccaac caccgcatgg tgggagaaga atacccccat gtcatcggtc
    17641 atgccttcgc aaacggctac cgggcctatc gcatcacgga acgattgcgc gacaaggacc
    17701 gcttccatga gacggattta ctggcgctgc aattggatac gacggcccag ttgtacgaat
    17761 tttatcgaga ccttgtgaaa cgtctgctga acgagtccgt catcgaggga acccccgatc
    17821 tggccgacgt gcgccgtgcc gtggaggctt gggagggcag ggccgatagc gatagcaaag
    17881 gcttcggcct gctggtggcg ttccgccgga cgttagcggc gtccgtcttc gcaccgttct
    17941 tacaaagttg ccgggaacag gacgcggggt tcgtctatga cggcgacctc gacacccctc
    18001 tgaggacatt gctgacggaa caggctcctg ccgttctgcc cgacccggct cgatttgcgg
    18061 actggaacac gttcctcctc caacagctga agcagaccgt ggaaacactc aagaccgact
    18121 acgacctgct cagtccggac gacctgacgt ggggaatcat gaatcgcgtg cgtatgaccc
    18181 atcccttgag cgaggcgatt cccggattag ggcgctggct ggatatgacg gatgaggaag
    18241 cgtccggttg cgggcaatgc gtgcgggtgc tcagcggaag ccttgcggcc agtcagcgga
    18301 tggtggtgtc gccctcgcac cattctgatg ccatctttca catgccgggg ggccaatcgg
    18361 ggcatcccat gtcggcacat taccgggatc aacagcgcaa ttggagcgag ggggttccaa
    18421 cgccgttgct cgcaggaagg ccgacccaca acctgacgtt gcagccgtcg gatcgtggcg
    18481 gggcgtcata gcgtcgcagt tcattctcac caacaaggag gcatcatggg acaggacgag
    18541 caggaagata cgacggtgta caaggtcgtg gtgaaccacg aagaacagta ttcgatctgg
    18601 ccttcttatc gcgaaaatcc attgggatgg caggacgccg gcaagaccgg cctgaaggat
    18661 gagtgtctgt cctacatcaa agacgtctgg acggacatgc ggccgctgag tttacgcaaa
    18721 tggatggcgg agcaggccga acaggcggac aagacggcgg ccccatgctg acggcggcgg
    18781 atcggcggcc atggatcgtc acctgccgtc gcggtgatgg tcttcggctg atctgcttcc
    18841 cctatgcggg gggcgggcca tcgctgttcc gcggttggcc gttggagctc tctcagcagg
    18901 tcgaggtctg tgccgtgcaa ctgccgggca gggaggcgcg gatgaaggag ccgccgatcg
    18961 gcgaccttcg tcggctggtg gtggagttgg ccgatgcgat cgaaccgtct ctcgaccggc
    19021 ctttcgccct cttcggtcat agcgtagggg gactggtcgc cttcgagttc gcgcgtgaac
    19081 tgcgtcgacg ctatggcatc gaacccgtgc acctcttcgt ctccggctgt cctgcaccgg
    19141 agttgaccga cgacgaacgc ctcagcgagt tgccggacgg ggagtttctc gaacggatgc
    19201 gacggttcaa cggcacgccg cgtgaaatcc tggagcatgc cgaaatgatg gagttggtcc
    19261 tgccgacgct ccgtgcagat ttctccttgc gcgataccta ccgccatcgc acggaatcgc
    19321 cgttgggttg tgcgatctcg gcattcggcg gcatggcgga ccgggctgtc ggagtcgata
    19381 agctgaatcc ctggagcgcg catacgaccg ggggatttca actgtggctg ttccaaggcg
    19441 atcacttttt tgtccgcacc gctcaagcgg cggtggtgga ggccgtgatg ttgctcctgc
    19501 aaccatacat gtcgctgcgg ctgtgatgca gatggtcggt agaacacacg cattcaacca
    19561 tgagtgggag gtattcgatg aaccatgaat caggatcgtc ctgcggaacg cgtcccggag
    19621 tgaacggcat agtgacgcgg aacggtggcc tcatgatcga gcccctctcg tccgacggca
    19681 gccttcccct ggtggtgaca ccggtagagg gcagaacctt ggacggctat ctcgccgccg
    19741 cgcgccatta tgccgaggca tttctgccgg tcagcggcgg cctgctcttc cgcggcctgc
    19801 cgatcggatc ggtcgctgat ttcgagtcgt tcgtcggtgg gttcacgtcg tcgttggtgt
    19861 cctacgaatt cggctcgacc ccccggtcgc gtgtgcagcg acaggtctac acttcgaccg
    19921 aatacccgcc gcatcaacat atcccgcttc acaacgagca ggcctacacg cgggaatggc
    19981 cgatgaagat ctggttttat tgcgggcagg cacccgagga gggaggctac accccgatcg
    20041 cggacagccg cgaggtctat cggcggattc cagcctgcat ccgggaacac ttcaaccgga
    20101 agcaggtcat gtacatgcgc aactatggca acggtctgga tgtgccttgg caaaaggtgt
    20161 tcaacacgga agaccctgca gtggtggaag cattctgccg gaccaatgga attctgtacg
    20221 agtggaaagc cgacggcgaa ttgcgcacca ggcaggtggc gcaggcgatc gcggtccatc
    20281 ctctgacggg cgaaacggtc tggttcaatc aggcgcattt gtttcatgtc tcgaatctgg
    20341 agccggcggt gcgtgaggac ctgctggccg tcgtggcgga ggaagacctg ccccgaaatg
    20401 cctgttacgg agacggcagc ccgatcgagt ctgatctgct ggatgaaatt cgcgacgtct
    20461 accggagcgt ggccgtgcaa ttctcctggc aggagggcga tgtgatgatg ctcgacaaca
    20521 tgctggccgc ccatggccgc acgcctttta agggccggcg gcagattctg gtggctatgg
    20581 cggaaccttg cagagccggt catccatgct gatgcctcac gaccagacgc aagacaggcc
    20641 ggtcgatgtc ctcggatcgg agccggagca gtacctgttt cctatgagtt tcgggcagcg
    20701 gcggctctgg tttctttcgc agttggaacc ggagagcgcc tcctacaaca cggccatcgc
    20761 cgtgcggatg aaggggaggg tggatcgccg gctcctggtg aattctttca accggatcgt
    20821 ggaacggcac gaagtcctgc gcacgagctt cagcaccgaa aacggggagc cggttcagat
    20881 catcgcgacc gtcggtcgag tcacactgga gaccgacctg gtgacgaatc agccggaagc
    20941 catcctggcc gcggcgcgtg acgaagcgca acgtcccttc gatcttcagc gggggccgct
    21001 gctccggttg cgtctgttcg acatcacaga cgacgaccac ttgctggtcg tcaccctgca
    21061 ccacatcgtg tgcgacggat ggtccgcgca gatcctggcg cgggaatttg cgcagctgta
    21121 tgtcgaggcg gtggagggga ccgatgcagc cttgcctgcg ctccccatcc aatatgcgga
    21181 ctatgcggaa tggcaacgcg ggtggttgca gggagaggtg cttgatcggc acctgaccta
    21241 ttggactcgg caactggctg gagacctccc ggtgatcgaa ctgccgaccg accgcccgcg
    21301 cggggctgtt caaacgtcgt cgggcagccg gtatgtcttt actctgccgc aggccctggc
    21361 cggggggttg cgggaactca gccggcgcca caacgtgact ctctttatga ccctgttggc
    21421 cgtgttccag acctggctca tgcgctatac gggcatcgac gatgtctgtg tcggcacccc
    21481 gatcgcctgc cggaccaaac gcgagacgga agatctcatc ggctttttcg cgaataccct
    21541 cgtcctgcgt acggaccttt cgcagaatcc gaccttccgg cagttgctcg aacgggtgcg
    21601 tgaagtcgtt ctcggcgggc aggagcatca ggagttgccg ttcgaagaac tcgtcgacgc
    21661 gctgaagccc gcgcgggccc tgagccactc tccgctcttt caagtgctgt ttgcgctgca
    21721 gaccacgctg gccgaagccg ttcgtttgcc cgggttgcac gtcgagatca ccgaggtcga
    21781 tgcggcagcc gcgaagttcg atctctcgct ggacatggcc gagacggagg cgggcctgga
    21841 gggagccttc gaatacaaca ccgacctgtt cgatgcggcg accgttgccc gcatggccgg
    21901 acatttccag accctgttgg ccggcatcgt cgcgacgccg gactgcagac tgcatgagtt
    21961 accgcttctg tcggtcgaag agcggcaaga ggctctgctg gtcgggcctg atgaccgcgt
    22021 cacagacgag tgcttcgact ttgccgccgt attctcggcc caagtcgaac gcagtccggg
    22081 tgccgtcgcc gtcgaatgcc ggggggcaac caggacgtat gccgagttga accgagaggc
    22141 cgaccggatc gccggcgcac tgaccacctc cggcatcggc ccggacaaga tcgtcgccct
    22201 gctgctcgac cgcggcattg gtctcctcac ggccatcgtg ggggtgttga aggcgggcgg
    22261 gacgtacctg ccgctggatc cctctcaccc tccggcccgt tggggcgaat tgattcactc
    22321 gagcgcggcg tccgtcgtcc tggcgaatga gtcctatcgc gctcgtgtgg gggaggcctc
    22381 tccacggttc gcatccggcg acgcgattct tcttgccctc gaggacgtcg tcggaaagcc
    22441 ggacaacgcg ctgcctcatc cttgcacctg ctcgccggac caattggcct acgtcatcta
    22501 tacgtccggc tcgaccggga ttcccaaggg tgccatggtc acgcagcggg gcttgatgaa
    22561 ccacctctgg agcaagcgcg attccttggg attgggcccg aatgacgtag tggcgcagac
    22621 ggcctcggcc tgtttcgaca tttccgtctg gcaacacctg gccgcgctca tctgcggcgg
    22681 gcggaccctg atcgtgccgg aggacgaggc ccgtgatcct gtgcagctct tgacctgtct
    22741 cgaacgcgat gcggtcaccg tcgccgaaac tgtgccggcg ctgctcaagg gcatggtgga
    22801 tggcccacaa gcagccgacg ttcccttgcg atcgttacgg tggctgttgc ccacgggtga
    22861 agcgctgccg ccgcagctct gccgcgagtg gctgcgacgg tatccccatg tgccgctggt
    22921 gaatgcctat ggcccggccg aatgtgccga tgacgtcgcg acggcgacga tcgcctcgcc
    22981 tccggccgcc gatgaggtct ctatgccgat cggacggccc atccaacgga tgcggctcgt
    23041 ggtggtggat cgatggctgg cgccggtgcc ggtcggggtt tccggtgaac tctgcatcgg
    23101 tggcgtcggg gtcggacggg gctatctgca ggatcctggt cgcacggctg ccgccttcgt
    23161 gcccgatccc ttcggaacgg agcctggtgc gcgcctctac cgaaccggag acctggtgcg
    23221 ccgtcgaaca gatggcagcc tggaatttct gggccgggtg gaccatcaaa tcaaattgcg
    23281 cggggtgcgg atcgaaccgg gagaaatcga tgcgcgcctt cgggagctgg cgggaattcg
    23341 ggacgccgtc acggtggtgc gagaagatcg gccgggccag aaacgcttgg tggcctatgt
    23401 cgtgacaagc gagcaggcgg cgccatcggt cgagacatgg cgcgccacac tccggtcaca
    23461 gttgcctgaa gtgatgatcc cgtcgcggtt cgtccgactg gataccctgc cccgcacgcc
    23521 gaacggcaag gtggatcgag cgtcgttgcc ggttcccgac gacccgggcg acgggggtta
    23581 cgcggctccc cgtgactcag tcgaagagca ggtggcgttg atctgggcgg aggcgttgca
    23641 acgggagcga gtcggaatcc acgacaactt tttcgagctc ggcggcgatt ccattctaag
    23701 tctgcaggtg gtgagccggg cccggcaggc cggtttcagc ctgacgccgc ggcaggtctt
    23761 tcaacaccag accgtggcgg aactggcggc actgttggcg cagccgagcg acgtctctcc
    23821 ggcgatcact gacgaagagc tctcggctga gctggtcgac gcagcgacgg gcgctgcggc
    23881 gaaggccgcc tgcccgaacc atgaggacgt ctatccactg agccctttgc agcaggggct
    23941 tctctttcac agtctgtatg ccccggcctc cggcgtctat atcgagcaga tgagttggcg
    24001 gatggcgggc gaactggacg tcccgctgtt ccgcgaggcg tggcagcaga tcgtcgatcg
    24061 tcacccgctg ttgcggacga ggtttctgtg gcgagaagtg tcgatgccga ttcagatcgt
    24121 gctgccggcc gccgagattg cttggcgcga ggtggatgcg cgggattgtg cgtcaaacga
    24181 acaggcggcc cggttcagcc gattagtgga ggaagatcgg ctgacggatt tcgacttcga
    24241 acggccgccc ttgatgcgga tcctgttgtt ccgcgtcggc cgggacacct acgacgtgct
    24301 ctggacgcac caccacatcc tcatggacgg atggtgtctg ccgattctgt tgcaggaagt
    24361 gttgaccgca taccaagccc tgcggcgagg agcggctccg ttgttcgaac cggtcgcgcc
    24421 ctatcgccat cacatcgttc ggctcctcgg acaggatcag gccgaggccc agcgattctg
    24481 gcgcaaggcc ctgaagggag tgaccacgcc aacggtcctg ggcgaagagg tcgcgcctga
    24541 ggaggtcggt caacaccgct caagcggcac ggccgccatc acgctttcgc ccgagaccag
    24601 tgcgcagttg cgcacgttcg cgcaggggca tcatgtgacg ctcaataccc tcgtgcaggg
    24661 cgcgtgggcg ctgttgttaa gccggtacag tcgagaatcg gaggtgctgt tcggtacgac
    24721 cgtgtccggc cggtccgccg acatacccgg gatcgaacgg atggtcggtc tgttcatcaa
    24781 cacccttccg ctgcgggtac gggtggctcc agacgccgtc ctctccgaat ggctgcgagc
    24841 tctgctggag cgcaatagcg aactgcggca attcgagcag acgccgctgg tgcagatcca
    24901 aagctggagc gatgtgcctc gggggcaagc cttcttcgag agtctgatcg tcttcgacaa
    24961 tcacccgctg gatgagtcgt tggagaaagc gaccgggctc accattcaag gcgtcacgct
    25021 ccaaggacag acgaactatc ccctgacact caatgtgtta cccggaaagg aactgacgct
    25081 ttcactctgg tatcaccgga accggttccg cgacgagact gctgcccgca tggtgcggca
    25141 gctcgagacg ctgctcaggg ccatgataca gaaccctcat gcacgcctgg gtctccttcc
    25201 cctgctggcg tcggcggagc gggcgcaggt ggtgggggcg tggaaccgga c
//
LOCUS       DAOQRM010000019        25077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1254_length_25077_cov_16.973703, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000019 DAOQRM010000000
VERSION     DAOQRM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25077
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_25077_cov_16.973703"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKK81_03170"
     CDS             <1..524
                     /locus_tag="PKK81_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNV31901.1"
                     /translation="IVFALNTGMRRGEILNLQWQAIDLNRQVLVVMRSKNGEKRTVPL
                     NGRLTDLLKRKASKRAAGSFVFASSAETLFDARNLTRSFRLAATKAALSDFRFHDLRH
                     TFATRLAQAGVDLYKVQRLLGHKTAAMTQRYAHHSPESLREGVLILERVHPQISQNYH
                     NEGGTVGECCASA"
     gene            complement(531..605)
                     /locus_tag="PKK81_03175"
     tRNA            complement(531..605)
                     /locus_tag="PKK81_03175"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(571..573),aa:Gly,seq:gcc)
     gene            889..1218
                     /locus_tag="PKK81_03180"
     CDS             889..1218
                     /locus_tag="PKK81_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31902.1"
                     /translation="MTSRPQPSAPFAAQAIPFDEYLASGKIPEGLLTSEYIGQQFVER
                     LVHYVLSVPAGSYTMAQLSRLLEELDPRAQVFFFKRLKENSPDSLKDFAPLYYGFMNE
                     FHSLLFT"
     gene            1303..1671
                     /locus_tag="PKK81_03185"
     CDS             1303..1671
                     /locus_tag="PKK81_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31903.1"
                     /translation="MNPTLQRAVTSFYNLVYEAQTFATTMSRIDTAEQEHYAGRIEGL
                     NWVLDRCQELEDMDANITPTTLQRVLTEVKSDLDHELSVQRREKGRRADGREEALTFV
                     ADYLSSLITATEIESAKTSV"
     gene            1729..1968
                     /locus_tag="PKK81_03190"
     CDS             1729..1968
                     /locus_tag="PKK81_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31904.1"
                     /translation="MAREWIIFAVCLGLGGHIALAVVLHAPDLWPWSKAWLYGLLSGV
                     ALYVVVQVIRSLWWIFRGRNAEEPETPEDQRIGHP"
     gene            complement(2480..2776)
                     /locus_tag="PKK81_03195"
     CDS             complement(2480..2776)
                     /locus_tag="PKK81_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNV31905.1"
                     /translation="MRDIVSDAAGKKVNLDKDLLAILCCPETKQAVALADEQLIQKVN
                     GAIERGALKNKAHKPVTEKLDGGLIRSDNKILYPVREDIPVMLIDEGIPLDQVS"
     gene            2920..3546
                     /locus_tag="PKK81_03200"
     CDS             2920..3546
                     /locus_tag="PKK81_03200"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNV31906.1"
                     /translation="MTASHRLGRIFLTGLLVLLPAWTTFLILAALFETLDSFLLNLIG
                     RQIQPYAPGLGLLLLIGMVLTVGAIATQVIGQRVVRWTESVLDRIPLIRSIYMTLKGM
                     TDLLNYRARFGQSTVVAFPFPRDGLWALGFVMGSPPAALQIAPMVELVMVFVPTAIHP
                     FTGYLALIPKTQLHPLNLLPEEALKLEFSAGLYRPLPGWLTSPARNPS"
     gene            3543..4061
                     /locus_tag="PKK81_03205"
     CDS             3543..4061
                     /locus_tag="PKK81_03205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV31907.1"
                     /translation="MSLADSLTVRPATLEDLDALTTFSAAMALETEQRTLDHARLRLG
                     TRAVLEQPERGRFYVADLRPEPPADRVTVGQLLLTYEWSDWRNAQFWWIQSVYVHPDW
                     RRKGIYRSMHGAIINLARSQPGVCGVRLYVEGDNHIAKCVYERVGLSPSSYQVYESDF
                     VLPTPHSARTKA"
     gene            4097..4477
                     /locus_tag="PKK81_03210"
     CDS             4097..4477
                     /locus_tag="PKK81_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31908.1"
                     /translation="MRRLSVLLVLMLLTLNACAFSRGTLGDEIKTETVNSIKKGVTTR
                     SEVLALLGAPDRLLQLNGRDLFQYYRYDAKVGSLLLIVLNFSRVSVKSDDLFVVINRE
                     GTVEEVIASKRTEGLEFRFWPFGD"
     gene            4482..4904
                     /locus_tag="PKK81_03215"
     CDS             4482..4904
                     /locus_tag="PKK81_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31909.1"
                     /translation="MTGWNRSVTLLFSAVIALALSGCNVARLTINTPLTPESVAFIAE
                     GRTTLADVVDTLGAPDSITDTETGTIVTYRFLNAKYSRVNFGWLLKPWSPVDPDLVFS
                     RSGLGTDAFELLCDDNWIVTHHAFVRHRPAPPFTPYPF"
     gene            4972..5829
                     /locus_tag="PKK81_03220"
     CDS             4972..5829
                     /locus_tag="PKK81_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNV31910.1"
                     /translation="MGAGSSDESSSQQAPGTSSSYIPADHDIDFLQRDELRPLRLGLE
                     LLKPELIQTEQGIRSTIVVFGSARLLEPSRARQALEQASAALQACPDDRTCQQRVAVA
                     ERRLALSRYYDVAREFGRLVSSTCQIDGQCDYVIVTGGGPGIMEAANRGAADAGAKSM
                     GLNITLPHEQRPNPYITPDLCFQFRYFALRKMHFLLRARALVVFPGGFGTLDELFETL
                     TLLQTGKTRNITIILMGQAFWERLINWPLLIEEGLVGPEDLALFHFAETAQQAWDLIS
                     RSHGGPSAR"
     gene            5826..7223
                     /locus_tag="PKK81_03225"
     CDS             5826..7223
                     /locus_tag="PKK81_03225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV31911.1"
                     /translation="MKLSFHGAARSVTGSRHLLEMPGNRILLDCGLFQGQRQEADRQN
                     RFLGFDPRSIQAVLLSHAHIDHSGALPVLGKHQFRGNVHMTRATVDLAAVMLEDSARL
                     QENDCAYLNKKENRRGKRCLQPFYESRDARAIIRRFVGARYGDTIKVTPRVTASFHDV
                     GHILGSAAIRLKYSARGNSTTVLFSGDLGRSQMPILRDPAPPPSCDVLIIESTYGDRL
                     HEEAGEALTQKAQQLVAHAKAHKSKIIVPAFALGRTQDLVMRIKRLVAEGRIDPLPIY
                     IDSPLASKVTDVFRKHPECYDEETFRTFTSEGDPFAARYIRYVSSPEESKRLNEMKGP
                     CVIIASSGMCEGGRVVHHLKHGIQDEANIIAIVGFQAEHTLGRRLVEGWDVVPIFGIP
                     TPRRAQVVVFNGLSAHADRNDLLAYVRAISPAPQQVFVVHGEEKQALSLGAAIQTEHP
                     GMAVVVPAKDSIYEI"
     gene            7268..8533
                     /locus_tag="PKK81_03230"
     CDS             7268..8533
                     /locus_tag="PKK81_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNV31912.1"
                     /translation="MNPSRKILNAVRACAVILLTACVSPAWCLDGSPDSVDQVRIRAR
                     ALGLHPGQFQPGPLNAITDVPGVKVGQVTLMQGDGPLQPGQGPVRTGVTVIVPRDDVW
                     HKKVPAGSFVLNGTGEMTGLSWVAESGFLEYPIALTNTLNVPRVANGVISWMLKQYPG
                     IGITDDTLTPVVAECDDGRLNDIQGRHVSETDVMRALDEASSGPVAEGSVGAGTGMIS
                     YGFKGGIGTASRRLPEANGGFTIGVLVNANHGRRPELTMGGVPVGQRYGAALTQSSLS
                     PEQNSSSLHASREGTSGNAEGSIIVVIATDAPLDGRQLTRLGKRAALGLARTGSTARH
                     GSGDFMLAFSTGNVIPHYPSDPTFSLTHLADTHLNPVMTATVEATEEAILNALTGATT
                     VTGRDGFRAEAISIPRLRELLSAGATPGP"
     gene            8629..8856
                     /locus_tag="PKK81_03235"
     CDS             8629..8856
                     /locus_tag="PKK81_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31913.1"
                     /translation="MADYPIGGGLTLLTALEKNGKFAEFLQTRMTQALETQDPTELHY
                     LLAQLDDYHSYMWRYYKKLASDRPERMNPGV"
     gene            8905..10131
                     /locus_tag="PKK81_03240"
     CDS             8905..10131
                     /locus_tag="PKK81_03240"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNV31914.1"
                     /translation="MILTPPSTLRFASALTRQADAQAAADELIRAIRVQLGSSRIDVA
                     FLFISAQHADQADTLSHALRTALGPDTLVGCTGEGVIATGREVETGPAATLWAAHLPG
                     VIAHPLRLSFSSVHDQFSLRDWPELDYGGESAPVMLLFADPFSTPLQDVLGVIEERYP
                     HARALGGLAGGGQDLAENRLFLDDEVYSDGLVGVVLSGNISVRTVISQGCRPIGDRFI
                     VTKAEHNVIQELGGIPALHCLQTVFGQLSMDERAQAQRALHIGIAMDEQRAQFTRGDF
                     LIRNLLGADQQTGAIVVGDVVQEGQTVQFQVRDAQSADEDLHALLAASRLEESQRPLG
                     ALLFSCCGRGKGLFGVPNHDASVLGEQLGAIPLAGFFAQGELGPVGGRNFLHGYTASI
                     AIFSEPDRSDTQGSNH"
     gene            10263..10634
                     /locus_tag="PKK81_03245"
     CDS             10263..10634
                     /locus_tag="PKK81_03245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV31915.1"
                     /translation="MAQSEQTASTPEITTVSGLTYTDVVVGTGREAAAGNLVTVHYTG
                     WLTNGKKFDSSVDRSEPFSFPLGAGRVIKGWDEGVAGMKVGGKRKLTIPSQLGYGARG
                     AGGVIPPNATLVFDVELLEVR"
     gene            10690..11799
                     /gene="gcvT"
                     /locus_tag="PKK81_03250"
     CDS             10690..11799
                     /gene="gcvT"
                     /locus_tag="PKK81_03250"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNV31916.1"
                     /translation="MKQTPLIDAHRKAQGKLVDFAGWEMPIQYSGVVDEYQTVRRHAG
                     LFDVSHMGRISVSGPGSLAFLQRVTTNDVSKLSVRQSHYSMICAPNGGIKDDIFIYHV
                     KPYEFLVCVNASNREKIVAWLHEKVAQAQGCKVQDQSASLAQIAIQGPASRDILAAAG
                     IADLSTLKIRHCLDATLGGHSLLVTRTGYTGELGYELYLPAAGAPTVWDRLLEAGRPL
                     GIKPAGLGARDLLRLEMAYLLYGNDMNEETTPIEAGAEWVVKFDKGDFIGRTELLAQQ
                     SQGAARRLVAFELVEKAVPRHGFKILSAQVPHAEIGEVTSGNLSPLLQKGIGMGYVTP
                     AAAQPGSSILIDIRGKACPAVVVKPPFYKKPAGTP"
     gene            11803..12321
                     /locus_tag="PKK81_03255"
     CDS             11803..12321
                     /locus_tag="PKK81_03255"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV31917.1"
                     /translation="MVKPIYKGTVVTLNVDTVRLPNGHTIDLEVIRHPGASAVVPLKE
                     DGTVVLIRQFRHAANGFIYEIPAGKLHPKEDPLDCAARELEEEIGYKAGRFELLSSIF
                     TAPGFADEVIHIYLATGLTIGTQNLDQDEVLEVVEMPLREAIAKIEDGTIRDGKTIVG
                     LQAVYIRQERYR"
     gene            complement(12398..12622)
                     /locus_tag="PKK81_03260"
     CDS             complement(12398..12622)
                     /locus_tag="PKK81_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31918.1"
                     /translation="MKKMMLTVMAAALLVAFSAPSFAGDDKKKEEKKGGHFSQTLFGD
                     DKKKEEKKGGHLSQTVFGEEKKKDEKKGGH"
     gene            12862..13407
                     /locus_tag="PKK81_03265"
     CDS             12862..13407
                     /locus_tag="PKK81_03265"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HNV31919.1"
                     /translation="MPEQPPLPDHLRPELSIVFVGINPGLRSAALGHHYAGPSNRFWK
                     LIYDANLVPEPLSYRDDGRLPEWGLGLTNLVSRSTASMESLTPQDYTAGRLVLMRKLL
                     RYRPRVVALLGMTLYPILFPLEPKQTGRKPGLQPATLYDSNIVLLPNPSGRNASYPYH
                     SMLEAFRTLTPWAKSGLKSPP"
     gene            13507..14148
                     /locus_tag="PKK81_03270"
     CDS             13507..14148
                     /locus_tag="PKK81_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31920.1"
                     /translation="MQCFPITMSLAIALLFVAEPASAGASVQQLTAGALHECALGRQA
                     QTRAERVQHFAQGQAFGEQAVEKDDLSPDAHFSLFCNLGEQLRIDGESLTSLFGFRRM
                     MKELNRTLELAPDHLDALSAKGTVLVRIPAFMGGDKEKGETLLRHVIAREPAAVNARL
                     SLAKSYCANGRHEEAVAIATTALDLAQSLQRADFIPEARQVLSQLRAQAAKGN"
     gene            complement(14191..>14654)
                     /locus_tag="PKK81_03275"
     CDS             complement(14191..>14654)
                     /locus_tag="PKK81_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNV31921.1"
                     /translation="LTTILNPAPAAPLPSDLLRLVDILTPNESEAQVLTGSTDPAEAA
                     QILTGRGVGTVVVTCGANGAFLTTGNDVTHIPGFLVVTIDSTGAGDAFNGALACAVAE
                     GVPLKSAIERANAAGALATTGRGAQESMPTKDDIEQLCRSGVRQMEPRQRS"
     assembly_gap    14655..14754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14755..15272)
                     /locus_tag="PKK81_03280"
     CDS             complement(<14755..15272)
                     /locus_tag="PKK81_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNV31922.1"
                     /translation="MILVVGSSNIDLVASVDRLPSRGETVLGYRFAQSFGGKGANQAV
                     AAARAGAEVAFLSKLGADANGRLIEQHLAAQGLSRPILLRDAEFPTGVAMILVDHSGE
                     NQIAVVPGSNGRLTPADLRQHRELIAGARVLLLQMEIPRETVFEALRLGRECGLTTIL
                     NPAPAAPLPSDLL"
     gene            complement(15269..16255)
                     /locus_tag="PKK81_03285"
     CDS             complement(15269..16255)
                     /locus_tag="PKK81_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNV31923.1"
                     /translation="MQRPSPTKVILDTDPGVDDALALLLAFASPELQVVGVTTVCGNV
                     PVGQATKNLFRLLNVVQPPPGLLVGQGAARPLEEDLVTAVQVHGSDGLGELDSVLAEE
                     GTPRYPQVRLPSVLSTAQDVWNECVRRYPDEVTLITLGPLTNVAVALKVNPLTVQKFR
                     SVIVMGGAIGVPGNVAPAAEFNMYVDPHAAHRVFQASLPLTLVPLDVTTRVGVTRESL
                     MTWAAESRAPLARMTTDMTRKAFEFAEKVEGHGLFYFHDPLAVLAAVDSSLLKVEPLH
                     VSVEMAGRVSRGITIADRRPRTPEEKARPNMRVAVDVDVDRTLSFLRNRLCP"
     gene            complement(16288..16926)
                     /locus_tag="PKK81_03290"
     CDS             complement(16288..16926)
                     /locus_tag="PKK81_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31924.1"
                     /translation="MGGASSTDTATYRLPQVHAVLSIAAGCLLLPLFFSPRAWADSES
                     GAAAEMQQSSPMLDVDPGVTYRAGARLRIPGTDWSFLVPTGWQSTRPEDSEMPFLMAE
                     EGKGLGMMFPLTDVTRETVRDHLSQPLSLLHGLSFIPAGSEVETELSIARSYQGEDLA
                     GRALAVLGPGNTSVIYFLMGPPDEASGYQSVLEGLGQATRFAAPVSGRDVGL"
     gene            complement(16934..19819)
                     /gene="gcvP"
                     /locus_tag="PKK81_03295"
     CDS             complement(16934..19819)
                     /gene="gcvP"
                     /locus_tag="PKK81_03295"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNV31925.1"
                     /translation="MPQPNFLEPNDTFVPRHIGPTDSDIQEMLATLSLPSLEALVEVT
                     VPSDIRLQTSLTVPSPRGEQQVLAELRGLAGQNQVWRSLIGMGYYDCITPLVIQRNIL
                     EDPGWYTQYTPYQAEIAQGRLEALVNFQTMVADLTGLPLANASLLDEATAAAEAMTMC
                     AAMSRAAGHERKKFFVSENCHPQTIAVVQTRAEPLGIVLQIGAIQSLDLSRDEFFGLL
                     LQYPSTDGYVGDYSEFITRAHASGAYVVVATDLLALTLLRSPGEFGADVAVGSSQRFG
                     VPLGFGGPHAAFLATKEEFRRQMPGRIIGVSKDVTGRVAYRLSLQTREQHIRREKATS
                     NICTAQVLLAVMAGMYAVYHGPAGLRRIAERIHGLTMVLAEGLRRHGCAVGLEPVFDT
                     LRVPLSPAQSDTILNRARQQKINLRQYDDQSLGLSLDEWSTVEEVQQVLALFVGHEIP
                     AEEFQAILASVDVRYPAPLARTSPYLTHPVFHRYHAEHELLRYIHRLQSRDLSLVHSM
                     IPLGSCTMKLNATAEMLPVTWPEFGRLHPFAPSEQAQGYQTLFQQLEAWLAELTGFAA
                     ISLQPNAGSQGEYAGLMVIRAHHRHRGETQRDVCLIPVSAHGTNPASASMCGMTVVPV
                     ACDEHGNVDLNDLEAKATQHRNRLAALMITYPSTHGVFEEGIRRMCQLVHTHGGQVYM
                     DGANMNAQVGLCRPADLGADVCHLNLHKTFCIPHGGGGPGMGPIGVARHLVPFLPGHP
                     VTKLGGPESIGPIAAAPYGSPSILTISWVYIALMGREGLTKATQVAILNANYMAKRLE
                     KYYPVLYSGTRGFVAHEFILDLRPLKESSGVEAMDVAKRLMDYGFHAPTVSFPVAGTL
                     MIEPTESEVKAELDRLCEALIAIRGEIQSIAEGRQPRAGNVLKNAPHTALSVTAAEWT
                     KPYSREQAAFPAPWVRDNKFWPSVGRIDEAYGDRHLFCTCPPMDPAS"
     gene            20024..>21171
                     /locus_tag="PKK81_03300"
     CDS             20024..>21171
                     /locus_tag="PKK81_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNV31926.1"
                     /translation="MITHDILIVGAGLAGMRAAIAAPPDLNVALLSKVHPVRSHSVAA
                     QGGINAAIGEQDSWEAHAYDTAKGGLYLGDQDAIEAMCREAPQDILELERMGVIFSRT
                     PEGRIAQRPFGGAGFPRTCYAADRTGHALLHAMYEQLMKRRCRVYEEWYVTALLVEQG
                     RCCGVVAWDLVHGGLQVLRAKAVILATGGSGRVFSTSTNAVINTGDGMALAYRAGLPL
                     EDMEFVQFHPTTLKDTGILITEGARGEGGYLLNTLGERFMKRYAPEQMELATRSTVSL
                     AIGQEIQEGRGVDGCVLLDLRHLGRARILERLPQIRELALEFAGLDPIETPIPIRPGA
                     HYQMGGVKTNAWGETDLPGLFAAGECACVSVHGANRLGGNSLLETIVFG"
     assembly_gap    21172..21271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21376..22689
                     /locus_tag="PKK81_03305"
     CDS             21376..22689
                     /locus_tag="PKK81_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV31927.1"
                     /translation="MATVHPGTATSASAIASPLDSTQAVVCPFCQTLTPCAPVAGVPL
                     HQCPCPSCNENLLILNPDGQPADEDMVTPSSAGGSDATDMSRRLVGLAVALLACVLNY
                     ALLAGMASWMFNQIKSTHDPYDIDELTLSPIILKANGWTALHLAAARGDVSEVTSLLE
                     DGSPVDRHNNNGRTALFEAATRGQTPVVTVLLQYGANPNARMKQNVTALLTAAEHGHA
                     DTIAVLLSNGAEINALCTCGDSALHRAVRKGHLAAAQVLLEQGIAANQKSHGETALEI
                     AQHDEDRELIELLRAHGGREFSQAKAHRAQGVAFQKKGQVDKALFAYAEALNLDPDDP
                     EAYFDRGTALLEKDSPDEALIAFHAAIRLNSTFIEAYSAAASVHVARRQWDQASALWD
                     RYLAQRPQDGRAHFERAIVKRSKGDSKGFLQELQQACTFGYRAAC"
     gene            22951..23961
                     /locus_tag="PKK81_03310"
     CDS             22951..23961
                     /locus_tag="PKK81_03310"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HNV31928.1"
                     /translation="MQRGSFHGAGGTLLGMQIVNVCLTIVTLGAYHFWAKAKIRRYLF
                     SQTAFAGDRFAYHGTGKELYQGFLKATVVFGIPYFSLGAAHSFLALPQSVDFLLQAMA
                     ALVLFLYVPVAIVNARRYRCTRTSWRGIRFSFRGRTVDFLKLYFKGWLFTLLTLGTYY
                     PYFQTQRQAFLHSHTYFGNQRFRFTGHGSGLMVPFAVTLFTTYAVLCLCGLALALQLT
                     NAGLTLLLIPFVLGPVWVWLLGQKQKYYWDHTTFGEARFSSSITWQKLFGLYLGNLAL
                     LLLTLGWAWPWVTVRNARFFTGTLSLQGVTDLDRVLQDTTETSVTGEGLSNLLDTGFD
                     MD"
     gene            23967..>25077
                     /locus_tag="PKK81_03315"
     CDS             23967..>25077
                     /locus_tag="PKK81_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV31929.1"
                     /translation="MPTSRSAHYLDGRTATRHRVTLTVTPATLHIAMPDGSAKQWPYD
                     EIRQTQGTYRGEPVRLEFGPEPAEAIVISTAALLDDIHEAAPALARHFHNPARRHARI
                     GWTLCAAVGVVLMVVGLYRWGIPGIASAATPYIPVAWEESLGRQVVAHLAPETQQCRD
                     PERLRKLDHIVQTLAATHPASPYRITLSVVDAPTVNAFAAPGGRLVILRGLLEQTASP
                     EQLAGVLAHELQHVYQRHTTKAILEQTAATLLLTAVSGDMSGGLAWGLEGARTMGSLH
                     YTRNHETEADIEGLRMMQAARLDPAAMIAFYGTMERGAQDHAGPPDFLSTHPDMGERL
                     ATLIALAGPSPSDAQRLLPGEDWKDIRTLCRLQAGG"
BASE COUNT         5219 a         7861 c         6938 g         4859 t
ORIGIN      
        1 tcattgtctt tgccttaaac accggcatgc ggcgaggcga gattctcaac ttgcaatggc
       61 aagccataga cctcaatcgg caggtcctgg tggtgatgcg aagcaagaat ggcgagaagc
      121 ggactgtccc gctcaacggt cggttaacgg acttgctgaa gagaaaagcg tcaaagaggg
      181 cagcaggctc gttcgtgttt gcgagttcag ctgagacgct attcgacgct cgaaacctca
      241 cgcgatcatt cagactcgcg gcaacgaagg cagcgttgag cgatttccgc tttcacgatt
      301 tgagacacac ctttgccact cggctcgcac aagctggtgt cgatttgtac aaggtgcaac
      361 gtctgttggg gcacaaaaca gcagctatga cgcagcgcta cgcgcatcat tcgccggaga
      421 gtttaagaga aggagttttg attctggagc gcgttcaccc tcagatatca caaaactatc
      481 acaatgaggg tggaaccgtc ggagagtgtt gcgcaagtgc ttgagaaaat tggagcggga
      541 aacgggattt gaacccgcga ccctcgcctt ggcaaggcga tgctctacca ctgagctatt
      601 cccgcttcca ccgacagaag aagcgggatt ctaccggcca tgcgtaaaga ctgtcaagcc
      661 gggcagcaga aatatcgtca tgacagcgac tctacgtagc tgcctacgcg gggccactgt
      721 caaagccggc catctgcacg aagcgcgggt ggcccctttt ctcgcaaaca tacagccttt
      781 cgactcagac ctgcgtgctt cttctgtcca cttgacggac ctgcggggct tgagtagagt
      841 aattccaatg tcggcccact ccatcctcat cagcgaggta cccttagcgt gacctcacgt
      901 cctcagccgt ccgcgccttt tgcagcacaa gccattccct tcgacgagta tctcgcatcg
      961 ggcaagatcc ccgaggggct tctcacgagt gaatacatcg ggcaacagtt tgttgagcga
     1021 ttggttcact acgtactgag cgttcctgcc gggagctaca cgatggcaca actcagccgt
     1081 cttctcgagg agctggatcc acgcgcccag gtcttctttt tcaagcgact gaaagaaaat
     1141 agtccggata gtctgaaaga ttttgccccg ctgtattatg gattcatgaa cgagtttcat
     1201 tccctcctgt ttacgtaagt cactgcccag taaaaaaata ccttgtcggc cccccattct
     1261 caggtataat cacgagaacc tcttaatgag ccaaggagca ctatgaatcc cacgttacag
     1321 cgcgctgtta cgagttttta taatctggtg tacgaagccc agactttcgc gaccaccatg
     1381 tctcggatcg acactgccga gcaggagcac tatgccgggc ggatcgaggg gctgaactgg
     1441 gtcctggacc gctgtcagga gcttgaggac atggatgcca acatcactcc tacgacactg
     1501 caacgtgtcc tgacggaagt gaaatcggac ttggatcatg agctctccgt gcagcgtcgc
     1561 gaaaaaggcc gacgggccga cggacgcgag gaagccctga ccttcgtggc ggactacttg
     1621 tccagcctga tcacggcaac tgagatcgaa tccgccaaaa cctccgtgta accgcctgtc
     1681 gattgtctcc aggcttatcc ttcgtcatgg cctcctccga gggcgactat ggcccgtgag
     1741 tggatcatct tcgccgtttg tctcggcttg ggaggccata ttgcccttgc cgtggtgttg
     1801 cacgcgcctg acctgtggcc atggagcaaa gcctggctct acgggttgct ctcaggcgtc
     1861 gcgctctatg tcgtggttca ggtcatccgt tcactctggt ggatttttcg cggcaggaac
     1921 gctgaggagc cggaaacacc cgaggatcag cgtatcggcc atccttgatt ccaatcaggc
     1981 aggcatcgtc ctgttgcacc acatccatct ccctcagtag aataccgctc atgcagccac
     2041 gttcgaacag acccgcgccg cccaattggc ctccaagcgc catgcgaccg cgccccgcac
     2101 cgtcacagcc agggcaacgc cagacagatc aatacgactc gtttaacgcc tccctgctgt
     2161 actgcggaca atgccgccag gcaactccta cacgtcaacg gctcctgctc gttctgccga
     2221 caggcaatct ctacgaatac ttctgtgctc aatgcggagc ctcaaccggc tcaaagacgg
     2281 aaagtgcttc ggcagaaggt ggactgctcg ccccgtaagc atacgtgccg caatccactg
     2341 tcaaacggtc actccagcac gtgcgttcac ccataacacg ccgaaccctt acgcctgagg
     2401 cattactact ccatgacgaa gcgcgaacgc ctgccgaggg aggactcgca ttggggaaga
     2461 gaggaccgag ggagggtaat caggaaacct gatcgagagg gataccttcg tcgatcaaca
     2521 tgacaggaat gtcttctcgt acggggtaga gaatcttgtt gtcggatcgg atcaatcctc
     2581 catcgagctt ctccgtaacc ggcttgtggg ctttattttt gagcgccccg cgctcgatcg
     2641 caccgttcac cttttgaatc aactgctcgt cggcaagagc cacggcctgc ttcgtttcgg
     2701 ggcaacaaag aatggctaac agatccttgt cgaggttcac cttttttcct gcggcatcgc
     2761 tcacaatatc tctcacaatc ccacccgata ggggtgactg aacggttacc ggaaagatag
     2821 tccaaatgaa tcgacagatc aagaagatac caaatcgccc ccctttctga tatcgtcatt
     2881 gatctattcc tccttcactc accaacatcg gggcgcgacg tgactgcttc gcatcggctc
     2941 ggacgcattt tccttaccgg actcctcgtg ctccttccgg cttggacaac gtttctcatt
     3001 ttggccgcgc tgtttgagac actggattcc ttcttgctca atctcatcgg ccggcagatc
     3061 cagccctatg ccccgggact cggcctcctg ctgctcattg gaatggtact cacggtcggg
     3121 gccattgcga cccaggtcat cggacaacga gttgtacgct ggaccgagtc cgtcctggac
     3181 cggattccct tgattcgcag catctatatg acactcaagg gtatgacgga tttactgaac
     3241 taccgggcac gcttcggaca aagcaccgtg gtggccttcc cttttccacg cgacggcctt
     3301 tgggccctgg gttttgtcat gggctctcca ccggcggcac ttcaaatcgc accgatggtg
     3361 gaattggtga tggtctttgt cccgactgcc atccatccct tcacgggata cttggccttg
     3421 attcccaaaa cacaattaca cccgctgaac ctgttgccgg aagaggcatt gaagttggag
     3481 ttttcagcgg gactctatcg ccccttacca ggctggctga cgtcgccggc acgtaaccca
     3541 tcatgagtct agccgatagc ctgaccgtac gtcccgccac actggaagac ctcgacgcgc
     3601 tcacgacatt cagcgccgcc atggccctgg aaactgagca acgcacactg gatcacgcac
     3661 gcttacgcct gggaacccga gcggttctcg aacagcctga acgagggcgg ttttacgttg
     3721 cagacttgcg accagagccg ccagccgata gagtaaccgt aggccagctg ctcctcacgt
     3781 acgagtggag cgattggcgg aacgcccaat tctggtggat ccagagtgtg tacgtacacc
     3841 ctgactggcg acgcaaaggc atttatcggt cgatgcatgg cgccatcatc aacctcgctc
     3901 gatcacaacc gggtgtgtgc ggtgtccgcc tgtacgtcga aggcgacaat cacatcgcca
     3961 aatgcgtcta tgaacgagtc gggctctcgc catccagcta ccaggtgtac gagtctgatt
     4021 ttgtcctacc caccccccac agcgcccgca caaaggcgta agttagtccg atcgacgtcc
     4081 gcataaggag gatcacatgc gacgactgag tgtgctgttg gttctgatgc tcctgacact
     4141 gaatgcctgc gcgttcagcc gaggcaccct cggcgacgag atcaagaccg aaaccgtcaa
     4201 ctccatcaag aagggagtga ccaccaggtc cgaagtcctg gctctactcg gtgctcctga
     4261 ccgactcctt caactgaacg gacgtgatct ctttcagtac taccggtacg atgccaaggt
     4321 cggcagtctg ctgttgatcg tgctcaattt ttcgcgcgtg tcggtcaaga gcgacgatct
     4381 gtttgtggtg atcaaccgcg aagggaccgt cgaggaagta atcgcgtcaa aacgaacaga
     4441 gggcctggaa ttccgtttct ggccattcgg agactaaggt catgacaggc tggaaccgct
     4501 ctgtcacgct gctgttctcc gcagtcattg ccctggcttt gtccggctgt aacgtggctc
     4561 gcctcacgat caacaccccc ctgacgccgg aatcggtagc cttcattgcg gaaggccgca
     4621 ccacactggc cgatgtcgtc gacacactcg gagcaccgga ttccattacc gatacggaaa
     4681 caggaactat cgtcacctac cggttcctca acgccaaata ttcgcgggtc aatttcggct
     4741 ggctactgaa accctggtcg cccgtggatc ccgacctcgt gttctcgcgc tccggcctcg
     4801 gcacagacgc gttcgaactc ctgtgcgacg acaattggat tgtcacccac catgcctttg
     4861 tgcgtcaccg ccccgcaccg ccgttcacac cctatccgtt ctgacccctc tgcacatacg
     4921 cctcgcatcg gtgaattgcc gccctcggca catctcgtta taatgccgct catgggtgca
     4981 ggttcttccg acgagtcatc gagccaacag gcccccggca catcatcctc ctatatcccg
     5041 gctgatcacg acatcgactt tctccagcgc gacgaactgc gccccctgcg cctcgggctg
     5101 gagttgctca agccggaatt gatacagaca gagcagggta tccgctcgac gatcgtggtc
     5161 ttcgggagcg cacggctgct ggagccatcg cgcgcacgcc aagccctgga acaagcctcg
     5221 gcagcattgc aggcctgtcc cgacgatcga acctgtcagc aacgtgtcgc cgtagccgaa
     5281 cgccgcctgg ccctctcgcg atactatgat gtcgcccggg aattcggccg gcttgtgtcc
     5341 tcaacctgcc agatcgacgg gcaatgcgac tacgtcatcg tcacgggtgg aggcccgggc
     5401 attatggagg cggccaatcg tggagccgcg gacgcaggcg ccaagtccat ggggctcaac
     5461 atcacgctgc cgcacgagca gcggcccaat ccctacatca cgccagacct ctgttttcaa
     5521 tttcggtact tcgcgctgcg caagatgcat tttctccttc gagcccgggc gctggtcgta
     5581 ttccccggtg ggttcggtac actcgacgaa ctctttgaga cactgaccct tctccagacc
     5641 ggcaaaacgc gcaacatcac gatcatcctg atggggcaag ccttttggga gcgcctcatc
     5701 aattggccgc tgttgattga agaaggcctg gtgggccccg aggacctcgc gctcttccac
     5761 tttgcagaaa cggctcagca ggcctgggat ctcatcagcc gaagccatgg aggcccgtcc
     5821 gcccgatgaa actctcattc cacggagccg cgagatccgt tacgggaagc cgccacctgc
     5881 tcgaaatgcc cggcaataga attctcctag attgcggact ctttcaggga cagcgccagg
     5941 aagcggatcg tcaaaaccgg tttctgggat tcgacccgcg ctcgatccaa gccgtgttgt
     6001 tatcccatgc ccatatcgac cattccgggg ctcttcccgt gctgggcaaa catcagttcc
     6061 gcgggaatgt gcacatgacc cgcgcgaccg tagatcttgc cgcggtgatg ctggaggatt
     6121 cggcccgcct acaggagaac gattgcgcct atctgaacaa gaaagaaaac cgtcggggca
     6181 agcgctgcct gcaacccttc tatgagagcc gcgatgcccg cgccatcatc cgtcgcttcg
     6241 taggggcgcg gtacggggac acgatcaagg tcacccctcg cgtcaccgcc tcctttcacg
     6301 acgtcggtca cattctggga tccgccgcca ttcgcctcaa atattccgcg cgcggcaaca
     6361 gtacgacagt gctcttctca ggtgacttgg gacggtcgca gatgccgatc ctccgcgatc
     6421 cggctccgcc gccaagctgc gatgtgctga tcattgaatc cacctacggc gaccgcctgc
     6481 acgaagaagc cggggaggcg ctgacccaaa aagcccagca actggtcgcc catgccaagg
     6541 cgcacaaaag caagatcatc gtgcccgcgt tcgcgctcgg acgcacgcaa gacctggtca
     6601 tgcggatcaa acgactggtg gcggaggggc gaatcgaccc gctcccgatt tatatcgatt
     6661 cgcctctggc ctcgaaagtg acagacgtct tccgaaagca ccccgagtgt tacgacgaag
     6721 agaccttccg cacgttcaca tcggaagggg atccctttgc ggcccgatat atccgctatg
     6781 tctcctcgcc ggaagaaagc aaacgattga atgagatgaa aggcccctgc gtgatcatcg
     6841 cctcttccgg catgtgcgaa ggagggcgcg tcgtgcacca tctcaaacat ggcatccagg
     6901 acgaagccaa catcatcgcc atcgtgggat ttcaggccga gcatacgctg gggcgcagac
     6961 tggttgaagg atgggatgtc gtgccgattt tcgggatccc gacgccgcgc cgtgctcagg
     7021 tggtcgtctt taacggattg tcggcccatg cggaccggaa cgatctgctt gcgtacgtgc
     7081 gggcgatctc tcccgcccct cagcaagtgt tcgtggtcca cggagaagaa aaacaagcct
     7141 tgtcgctggg agcggccatc cagacagaac accccgggat ggctgtcgtg gtcccggcca
     7201 aagacagcat atacgagatc tagccccgct gctcgggcac acctactcag ggaggagatc
     7261 ggcagtcatg aacccgtcaa gaaagattct caatgcggta cgggcttgcg ccgtgattct
     7321 tctgactgcc tgcgtgtctc cggcctggtg cctagacgga tcgcccgatt cggtggacca
     7381 ggtccgaatc cgcgcgcgcg cattggggct gcaccccgga caatttcagc ccggtccctt
     7441 gaatgccatc accgatgtgc ccggggtcaa ggtcggccag gtgacgctga tgcaagggga
     7501 cggccccctg cagcccggac agggaccggt tagaaccggc gtcacggtta tcgtgccacg
     7561 ggatgatgtg tggcacaaga aagtcccggc cggatccttt gtcctgaacg gcaccggaga
     7621 aatgacgggg ctctcctggg tcgcagaatc gggctttctc gaatacccca tcgcactgac
     7681 caacacactg aacgtgcctc gagttgccaa tggcgtgatc agctggatgc tcaagcaata
     7741 tcccggcatc ggcattaccg acgacaccct gacccccgtc gtggcggaat gtgacgatgg
     7801 acgcttgaat gacattcaag ggcgtcacgt ctcggaaacc gatgtcatgc gggccttgga
     7861 tgaagcctcc tcaggccctg tggcggaagg cagcgtggga gccggaaccg gcatgatctc
     7921 ctatggattc aaaggcggca tcggcacagc ctcgcggcgg ctccctgagg ccaatggcgg
     7981 atttacgatc ggggtgttgg tgaacgccaa ccatggccgc agaccagaac tcaccatggg
     8041 cggggtaccg gtcgggcaac ggtacggtgc cgctctaacc caatccagcc tgtcgccgga
     8101 acagaattcg agctcccttc acgcctcccg cgaaggaacc agcggcaacg ccgaagggtc
     8161 gatcattgtc gtcattgcca ccgatgcgcc gttggatggt cggcaactca cccgcctcgg
     8221 aaaacgagcg gcattaggac tcgcccgcac cggctcaacc gcccgccacg ggagcgggga
     8281 tttcatgctg gccttctcca ccggcaacgt catcccccat tacccgtccg accccacctt
     8341 ttcactcacc catctggccg acacccacct caatccggtg atgaccgcca cggttgaggc
     8401 cacagaagaa gcgattctga atgcgctcac cggagccacc accgtcaccg ggcgcgacgg
     8461 gtttcgagcc gaagccatct ccatccctcg tctgcgtgag ctcctatccg ccggtgccac
     8521 gcccgggccc taacagcccg ccatctcccc ggctgagccg tcgatacctt gcttttgcaa
     8581 ggccccacgg ctattctcct ggcttgtcag tacgtcatcc ggagggctat ggctgattat
     8641 cctatcggag gtggcctcac acttctgacc gcgctagaaa agaacggcaa gttcgctgaa
     8701 tttttacaga cgcgcatgac gcaagcactg gaaacccagg acccgaccga actccactac
     8761 ttgctcgccc agctcgatga ctaccattcg tatatgtggc gctattacaa gaaactcgcg
     8821 tcggaccgac ctgagcgcat gaatccgggt gtctaaccgc accggacagc tcacgaccct
     8881 gatcctattg gcaaagacag acctgtgatt ctcacacctc catcaacact gcggttcgcc
     8941 tccgccctca cccgtcaggc tgacgcgcag gctgcggcgg atgaattgat ccgagccatt
     9001 cgcgtgcaat tgggctcctc acgcatcgac gtcgccttcc tgttcatctc cgcccaacat
     9061 gccgatcagg ccgatacgct ctcgcacgca ctccgcacgg cactcggccc cgacaccttg
     9121 gtcggttgta caggagaggg agtcattgcc accggacgag aagtcgaaac gggaccggcc
     9181 gcaaccctct gggcggccca tttgcccgga gtcatcgcac atccgttacg actgtcgttt
     9241 tcaagcgtcc acgatcagtt ttcactgcgt gactggcccg aactggacta tggaggcgag
     9301 tcggcccccg tcatgctcct cttcgcagat cccttctcca ccccgctgca agacgtgttg
     9361 ggcgtcattg aggagcgtta cccgcatgct cgtgcgctcg gtgggctggc aggtggcggg
     9421 caggatcttg ccgagaatcg cctttttctg gatgacgagg tgtacagcga tggcttagtc
     9481 ggtgtcgtgc tctccggaaa catctcggtt cgcaccgtta tttcgcaagg ctgtcgtccg
     9541 atcggagacc ggttcatcgt cacaaaggcg gagcacaacg tcattcaaga actcggcgga
     9601 atcccggcct tgcattgttt gcaaaccgtc ttcgggcaac tctccatgga tgaacgcgcg
     9661 caagcccaac gggccctcca tatcggcatc gccatggatg agcaacgcgc gcaatttacc
     9721 cggggagatt ttctgatccg caatctactt ggagcagacc agcagacggg ggccatcgtc
     9781 gtcggtgacg tcgtccagga aggacaaacc gttcaatttc aagtcaggga cgcacagtcc
     9841 gcagacgagg atctccacgc gttactcgcc gcttcccgtc tggaggaatc acaacggcca
     9901 ctgggggcgc tgctgttcag ttgttgcgga cgcgggaaag gactcttcgg ggttcccaac
     9961 catgacgcgt ctgtcctggg agaacaattg ggcgctattc ctctggcggg atttttcgcg
    10021 cagggcgagt tgggaccggt cggcggacgt aattttctcc acggttatac ggcgagcatc
    10081 gccatctttt ctgaaccgga ccgatcagac acacaaggct cgaatcattg actaccgtca
    10141 gcgcatgcag gtcacgccac cggacctgca gactggacaa ggacacctct atgaacactc
    10201 gcttctggct gacgacttgc ggtatcgtgg gattcttatt cctgaccata gggagaccaa
    10261 tcatggcaca gagtgaacaa accgcctcaa cgcctgaaat cacaaccgtt tcaggcctta
    10321 cctacacaga cgtcgtagtg ggcacaggcc gtgaggctgc cgcgggaaac ctggtgaccg
    10381 tccactatac cggctggctg accaacggga aaaaattcga tagctccgtt gaccgcagcg
    10441 agccgttctc ctttccactc ggtgccggca gggtcatcaa gggatgggat gaaggcgtcg
    10501 cgggaatgaa agtcggcgga aaacggaagc tgaccattcc ttcgcaactt ggatacggcg
    10561 cgcgtggagc cggcggcgtg atcccgccta atgcgaccct cgtcttcgac gtggaactgc
    10621 tggaagtccg gtaggaccgt tttacacgcg caggcattca ctcaccaccg tggacgttag
    10681 acgcacctga tgaaacagac acccctcatc gacgctcacc gcaaagccca gggcaaactc
    10741 gtcgactttg caggctggga aatgcccatc caatattccg gcgtagtgga cgaatatcag
    10801 accgtccgcc ggcacgcagg actgttcgat gtgagtcaca tggggcgcat cagcgtcagc
    10861 ggaccgggct cgttggcatt tttgcagcgc gtcaccacga acgatgtgtc aaaactgtcc
    10921 gtgcgtcaat cgcattactc catgatctgt gccccgaacg gcggcatcaa agacgacatc
    10981 ttcatctacc acgtgaagcc gtatgagttt ttggtttgcg tgaacgcctc gaaccgggaa
    11041 aagattgtgg catggctgca tgagaaggtg gcgcaagccc agggctgcaa agtccaggac
    11101 cagtcagcgt ccctcgcgca aatcgcgatc caaggtccgg cctcacgtga catcctggca
    11161 gccgcgggca ttgccgatct cagcacgctg aaaattcgtc actgcctgga cgccacgctc
    11221 ggtggtcatt cactgttggt gacacggacc gggtataccg gcgagttggg atatgagttg
    11281 tatcttccgg cagcgggggc gccgacagtc tgggatcgac tcctcgaagc gggacgtcca
    11341 ctggggatta agccggcagg gttaggcgca cgtgatttgc tccggctcga aatggcctac
    11401 ctgctctatg gcaacgacat gaatgaggaa acaactccca tcgaggccgg ggcggaatgg
    11461 gttgtgaaat tcgataaggg agacttcatc ggccggactg aattgcttgc ccaacaatca
    11521 cagggagcag cacgacggct cgtggcgttc gagctcgtcg aaaaggctgt gccgcgtcac
    11581 ggattcaaaa ttctgagtgc acaggtacct catgctgaaa tcggtgaagt caccagcgga
    11641 aacctgtcgc ccttgctgca aaaaggtatc ggtatgggat atgtcacgcc cgctgcagcc
    11701 caaccaggat cgtccattct gatcgatatt cgaggcaagg cttgccccgc cgtcgtcgta
    11761 aaaccaccgt tttacaagaa acccgcgggc acgccgtaag tcatggtgaa accgatctac
    11821 aagggcacgg tggtcacgct caacgtcgat acggtgcgcc tgccgaacgg ccatacaatc
    11881 gatctggaag tcatccgcca tcccggtgcc tccgccgtcg ttcccctgaa agaagatgga
    11941 acggtcgtgc tgatccgtca attccgtcat gcggcgaacg gttttattta cgaaataccc
    12001 gccgggaaac tccatccgaa agaagacccg ctcgactgtg cggcccgcga actcgaagag
    12061 gagatcggct acaaggccgg tcgcttcgaa ctcctctcca gcattttcac cgcgccggga
    12121 tttgcggatg aagtcatcca catctatctt gcgacgggcc tgacgatcgg cacgcaaaat
    12181 ctggatcagg atgaagtcct ggaagtggtg gaaatgcctc tccgcgaggc cattgcgaaa
    12241 atcgaggatg gaaccattcg ggacggaaaa acgatcgtag ggctacaagc agtctatatt
    12301 cggcaggagc gataccggta gacgacccaa gaggtctccc acctccagga tgggagacct
    12361 cccaagccag ctagcctcgc cagaaacggt gttgtgttta gtggccgccc tttttctcgt
    12421 ccttcttctt ctcttcgccg aacacggtct gggacaggtg tccacccttc ttctcttcct
    12481 tcttcttgtc atcgccgaag agggtctggg agaagtggcc gcccttcttc tcttccttct
    12541 tcttgtcgtc accggcgaac gaaggagcgc tgaacgccac caacaatgca gccgccatca
    12601 ccgtcaacat catcttcttc attctgactc acctcctgtt aggtttgaaa ttaccaacga
    12661 ccgacggatg ttcggtaaag caaagtcctt gcctcataca cccacagatc taaactgttg
    12721 ttttttcgga catgtcatgc atcgcacttc gcaatgctac agaaagcaca gctcgattct
    12781 gtggcagaat agcctggacg atactaaacc gcctcatcag tcgattcagc agacgcgaca
    12841 aggtacgctc tcacgaccat catgccagaa caacctcctc tgccagatca tctccgcccg
    12901 gaactcagca tcgtattcgt gggcatcaat cctgggctac gctcagccgc gctggggcat
    12961 cactacgcgg gaccttcgaa tcgtttctgg aaactgattt acgacgcgaa ccttgtgccg
    13021 gaaccactca gctaccgaga tgacgggcga ctaccggaat ggggattggg actcaccaat
    13081 ctggtcagcc gatcgaccgc gagcatggag agtctcacgc cgcaggatta tacggccggc
    13141 cgacttgtgc tcatgcggaa acttctccgg tatcgcccgc gtgttgtcgc cctgctcggc
    13201 atgacgctct accccatcct ctttccactc gaaccaaaac agacaggacg aaaaccaggt
    13261 ctgcaaccag cgacactgta cgactccaat atcgtgttac tccccaaccc gagtgggcga
    13321 aacgcatcct atccctacca ttccatgctg gaggcttttc gtacgctgac accgtgggcc
    13381 aagagcggcc tcaagagtcc tccctgactt atcctattca cgccatgcgc tccgctggaa
    13441 ttccgtcggg gacctgctgt aaaatctgcg gctgatttcc ttcgggcaca ccgaaacgga
    13501 cactccatgc aatgttttcc catcacgatg agccttgcga tcgctctgtt attcgttgcc
    13561 gagccggctt ccgcaggcgc ctcggttcaa caactcacgg ccggtgcgct gcacgaatgt
    13621 gcgctgggcc gtcaggctca gactcgtgcg gagcgcgttc aacatttcgc gcaaggtcaa
    13681 gcgtttggag aacaggcggt cgaaaaagac gacttatcgc ccgatgccca cttctcgctg
    13741 ttttgtaacc tcggtgagca attgcggatc gacggggaaa gcctgacctc gctcttcggc
    13801 ttccgacgca tgatgaagga gttgaaccgc accctcgaac tggctccgga ccatttggat
    13861 gccctctcag ccaagggaac cgttctggtg cgcattccgg cattcatggg cggggataaa
    13921 gaaaaggggg agacgctgct gcgtcatgtc attgccaggg aaccggctgc cgtcaatgcc
    13981 cgattgagcc tggcaaagag ctattgcgcc aatgggcgac atgaagaagc ggtcgctatc
    14041 gcgacaacgg cgctggatct cgcgcaaagc cttcagcgcg cagacttcat tcccgaagcc
    14101 cggcaagtgc tgtcccagtt gcgagcccaa gcggccaaag gtaattgagc cacccgcttc
    14161 tgccactcgc ctgaaatcag gtctaccgac ttacgaacgc tgccgcggtt ccatctgcct
    14221 gaccccggat cgacataact gctcgatgtc atctttggtc ggcatcgact cctgcgcccc
    14281 acgtccggtg gtggccaacg caccggccgc attggcccgc tcgatcgcgc tcttgagcgg
    14341 taccccttca gccacagcac aggccaaagc accgttaaac gcatctccgg cgcccgtcga
    14401 atcaatcgtc acgacaagga aaccggggat atgagtcaca tcgttcccag ttgtcaggaa
    14461 cgccccgttt gcgccacagg tcacgaccac ggtgccgacg ccacgaccag tgagaatctg
    14521 tgccgcctct gccggatccg tcgaaccggt caacacctgc gcttcgcttt cgttgggagt
    14581 gaggatatcg accaatcgga ggaggtccga cggcaatgga gcagccggag ccggattaag
    14641 aatcgtggtg agacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaggagg
    14761 tccgacggca atggagcagc cggagccgga ttaagaatcg tggtgagacc gcattcgcgg
    14821 ccaagcctca acgcttcgaa aacagtctca cgcggaattt ccatttgaag cagcaacacc
    14881 cgtgctccgg ctatcaactc acgatgctgc cgcagatcag ctggtgtcag acgcccattg
    14941 ctccccggca cgaccgcaat ctgattctct ccggagtgat cgacgagaat catcgcaact
    15001 ccggtgggga attcggcatc gcgaagaagg atcgggcgcg acagcccctg cgcggccaag
    15061 tgctgttcga tcaaccgtcc attggcatcc gcccccagct tgctgagaaa tgcgacctcc
    15121 gctccggcgc gggctgccgc cacggcctga ttcgcgccct tgccgccgaa tgattgggcg
    15181 aaacggtacc cgaggacggt ttctccccgg ctcggcaggc gatcgaccga cgccaccaaa
    15241 tcgatattgc tggacccgac cactaggatc atgggcacag ccggttgcgt aggaaactca
    15301 gcgtccggtc gacatcgaca tcaacggcca cgcgcatgtt cggacgcgcc ttctcctccg
    15361 gcgtgcgcgg gcgacgatcg gcgatggtaa tcccgcgaga cactcgcccg gccatttcaa
    15421 ccgacacatg caatggctcc accttcaaca aggaggaatc gacggcggcc agaacggcca
    15481 acggatcgtg gaaatagaag agcccatgcc cttcaacctt ttcagcgaac tcgaatgcct
    15541 tccgcgtcat gtcagttgtc atccgagcga gcggggcacg cgactcagcg gcccaggtca
    15601 tcaagctctc ccgtgtcact ccgacgcggg tcgtcacatc gagcggaacg agcgtgagcg
    15661 ggagcgacgc ctgaaacacc cgatgcgccg catgcggatc cacatacata ttaaactctg
    15721 ctgcgggggc aacattgccg ggtacgccga tcgcgccccc catcacgatc accgaacgga
    15781 acttctgaac cgtcagggga ttcaccttca acgccacggc cacattcgtc agtggtccta
    15841 acgtgatcag cgtgacctca tcgggatacc ggcggacaca ttcattccaa acatcctggg
    15901 cagttgagag cacagaaggc aaccggactt gagggtatcg cggcgtcccc tcctcggcca
    15961 ggacagagtc gagttcgcca agcccgtcgc ttccgtggac ctggaccgct gtcacaagat
    16021 cctcctccaa aggccgcgcc gcgccctgcc cgaccaacag ccccggaggt ggctgtacaa
    16081 cgttcagcag gcggaacaaa ttcttcgtcg cctgtcccac cggcacgttc ccgcacactg
    16141 tggtcacccc gacgacctgc aattcgggcg aggcgaacgc cagcaacagc gccagggcgt
    16201 catcgactcc tggatcggta tcgagaatca ctttcgtagg agagggtctc tgcatgccta
    16261 gaagatttga tagggaatcg acccctatta caagccgacg tcacgcccgg ataccggtgc
    16321 ggcaaatcgg gtagcttgtc caagtccttc cagaacggac tgataccctg acgcctcgtc
    16381 gggaggtccc atgagaaaat atatcaccga cgtattgccg ggcccgagga ctgccaaggc
    16441 acgaccagcc aggtcttcac cctgatacga gcgggcgatc gagagttcag tctcaacttc
    16501 agagccggca ggaataaacg acaacccgtg taacagcgac aggggttgac tgaggtgatc
    16561 gcggaccgtc tcccgggtca catcggtgag gggaaacatc attcccaggc ccttcccttc
    16621 ttccgccatc agaaagggca tctccgaatc ttcggggcga gtactttgcc aaccggtcgg
    16681 caccaggaac gaccaatcgg tcccgggaat tctgagcctg gcgccggcgc ggtacgtcac
    16741 gccgggatcg acatcgagca tgggactgct ctgttgcatc tccgcagccg cgcccgattc
    16801 tgaatcagcc caagcccgtg gcgagaagaa caggggcaac agaagacaac cggcggcgat
    16861 cgaaaggacg gcatgcacct gcggcaggcg atatgtcgcc gtgtccgtcg aggaggcacc
    16921 gcccacagat cgattaggaa gcgggatcca tcggaggaca ggtgcaaaag aggtggcggt
    16981 cgccataggc ttcatcgata cgacccacgc tcggccaaaa tttgttgtca cgcacccacg
    17041 gagcaggaaa cgccgcctgc tcacgcgagt acggcttcgt ccactcagcc gcggtcacag
    17101 ataatgccgt gtgaggagca ttttttagaa cattgcccgc acgcggttgc cggccttcgg
    17161 caatcgattg aatctcgccc cggatcgcga tcaaggcttc gcacaaccga tccaactccg
    17221 ccttcacttc gctctccgtc ggctcgatca tcaaggtgcc ggccacggga aacgagaccg
    17281 tgggagcgtg gaacccgtaa tccatgagac gcttggccac gtccatcgct tccacaccgc
    17341 tgctctcctt gagcgggcgc agatccaaaa tgaactcatg tgcgacgaag ccacgggtgc
    17401 cgctatacaa cacggggtaa tacttttcta atcgtttggc catatagttg gcgttcagaa
    17461 tggccacctg cgtggctttg gtcaggccct ctcgtcccat cagggcaata tagacccagg
    17521 aaatcgtgag gatgctcggg ctcccgtacg gcgcggcggc gatcgggccg atcgactccg
    17581 gtccacccag tttggtgacc gggtgtccgg gcagaaacgg caccaaatga cgggcgaccc
    17641 cgatcggtcc catcccgggc ccgccaccgc cgtgaggaat gcagaacgtc ttgtgaaggt
    17701 tcaggtgaca gacgtccgcc cccaggtcgg ccggccgaca gagcccgacc tgagcattca
    17761 tgttggcccc gtccatataa acctgcccgc catgcgtgtg cacgagctgg cacatgcggc
    17821 gaatcccttc ctcaaaaaca ccgtgggtcg agggataggt gatcatcaac gccgcgaggc
    17881 ggttgcgatg ctgagtggct ttggcctcaa gatcgttcag atccacattg ccatgctcgt
    17941 cacaggccac cgggaccacc gtcattccgc acatcgacgc gctcgcagga ttggtcccgt
    18001 gcgccgacac tggaatcaag caaacgtcgc gttgcgtttc gccgcgatgc cgatgatgcg
    18061 cacggatcac catcaaccct gcatactccc cctgcgaacc ggcattgggt tgcagcgaaa
    18121 tggcggcaaa cccggtcagt tcagccaacc aggcttcgag ctgttggaac agcgtctggt
    18181 atccctgcgc ctgttcggac ggagcaaacg ggtggaggcg cccgaactcc ggccaggtca
    18241 ccggcaacat ttccgccgtc gcattcagtt tcatcgtgca ggatcccaag ggaatcatgg
    18301 aatgcacgag cgacaagtcg cgggactgaa gccggtggat gtagcgtaaa agttcatgtt
    18361 cggcatggta ccggtgaaac accggatgcg tgagataggg gctcgtgcgc gcgagcggtg
    18421 cgggataacg cacatcgacc gaggcgagaa tggcctgaaa ttcttcggca gggatctcat
    18481 gaccgacgaa gagcgccaac acctgctgga cttcctcaac cgtactccat tcatccaacg
    18541 aaagccccag gctctgatcg tcgtactgac ggagattgat cttctgctgt cgcgcccggt
    18601 tcagaatggt gtccgattgc gccggcgaca gtggaacacg caacgtgtcg aacaccggct
    18661 ccaggccgac ggcacaacca tgccggcgca aaccttcagc cagtaccatc gtcaggccgt
    18721 gaattcgctc tgcaatccgc cgcaaccctg caggaccgtg atagaccgca tacatccccg
    18781 ccatgacggc caacagcact tgcgccgtgc agatattgct ggtggccttc tcgcgccgga
    18841 tatgttgttc ccgggtctgc agcgacaagc gataggccac gcggcccgtc acatctttcg
    18901 agacgccgat gatccggccc ggcatctgcc ggcgaaactc ctccttggtg gcaagaaacg
    18961 ccgcatgggg cccaccgaat ccaagcggga caccgaaccg ttggctggat ccgaccgcca
    19021 cgtccgctcc gaattctccc ggtgaacgca gcagcgtgag cgccagcaag tcggtcgcca
    19081 ccacgacgta ggcccccgag gcatgcgcgc gcgtgatgaa ctcgctgtag tcaccgacgt
    19141 acccgtccgt cgaggggtat tgaagcaaca gcccgaagaa ctcgtcccgg gaaagatcca
    19201 acgactgaat cgcaccgatc tgcaggacga tgcccagcgg ttccgcacgg gtctgcacca
    19261 ccgcaatcgt ttgcggatgg caattttcag agacgaagaa cttcttgcgc tcatgcccgg
    19321 ccgctcgaga catggccgca cacatcgtca tcgcctcggc tgcggcagtc gcctcatcga
    19381 ggagcgacgc attggcgagc ggtaagccgg tcagatcggc gaccatggtc tgaaaattga
    19441 ccagtgcctc gagacgcccc tgcgcaatct cggcttgata cggggtgtac tgcgtatacc
    19501 atccgggatc ctccagaatg ttccgctgga tcaccaacgg cgtgatgcaa tcgtagtacc
    19561 ccatgccgat caaagaccgc cacacctgat tctgtccggc caagccacgc aattccgcca
    19621 ggacctgctg ctcaccccgc ggggacggca ccgtcaagga ggtctgcaag cgaatatcgg
    19681 acgggaccgt cacctcgacg agggcctcca acgacggcag gctcaatgtg gccaacatct
    19741 cctggatgtc ggaatccgtc gggccaatat ggcggggcac aaacgtatcg ttcggttcaa
    19801 ggaaattcgg ttgtggcatg ggggtgaaat cctgctaaaa gttgacgctc tcgtgcattc
    19861 gaagcggcgt gggctaacct atcatgcagg accgagtttt ccaagcgttc agcgcccatc
    19921 gcccgtcgct tgttttccga aattccatag cgtaatagtg acatcggatg tccctcccgg
    19981 gatgtcgatc gcacggctga acggtggcca ggatacgaag accatgatca cacatgacat
    20041 cttgatcgtc ggagcgggac tcgccggaat gcgggcggct attgccgcac cgcctgacct
    20101 caatgtggca ctcctctcca aggttcatcc ggtccgcagc cactccgtgg ccgcccaagg
    20161 cggaattaac gcggccatcg gggagcagga ctcctgggaa gcccatgcct atgacacggc
    20221 caagggggga ctttacctgg gcgatcagga tgcgatcgag gccatgtgcc gggaagcccc
    20281 gcaagacatt ttagagcttg aacgcatggg ggtgatcttc agccgcaccc cggaagggcg
    20341 aatcgcccaa cggccgttcg ggggagccgg atttccacga acctgctatg ccgccgaccg
    20401 caccgggcac gcgctgttgc acgccatgta cgaacagctg atgaagcgac ggtgccgggt
    20461 ttacgaagaa tggtacgtca cggcgttgct ggtcgaacag ggccgttgtt gcggagtggt
    20521 ggcctgggat ctggtccatg gaggattgca ggtcttgcgt gcaaaggccg tcattcttgc
    20581 caccggcggg agcggccgcg tcttttcgac cagcacgaat gccgtcatta acaccggaga
    20641 cggcatggcc ctggcctatc gcgcgggcct cccgctggaa gacatggagt tcgtccagtt
    20701 ccatccgacc accttgaaag acactggcat tctgatcacc gagggcgcgc gcggggaagg
    20761 cggctatttg ctgaacacgc tcggcgagcg gttcatgaaa cgatatgcgc cggagcagat
    20821 ggagctggcg acccgctcca cggtgtcgct ggccatcggc caggagattc aagaaggacg
    20881 aggcgtcgac ggctgcgtct tactcgacct tcgacacctc ggccgcgccc gtattctgga
    20941 gcggctcccg cagattcgag agctggccct ggaattcgcc ggactcgatc cgatcgaaac
    21001 acccattccc attcgacccg gcgcccatta ccaaatgggc ggcgtgaaaa ccaacgcttg
    21061 gggagagacc gacctaccgg gactcttcgc cgcaggcgaa tgcgcttgcg tgagtgtgca
    21121 cggagccaat cgcctgggag ggaattccct cctcgaaacc attgtcttcg gnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccggttgcg ctcctaccgt tttgcgctat
    21301 ccttttcaat aatcaccgat cgctccactc acgggaccgt ccacaacaag cccgggcgaa
    21361 gggtcgttag gcaccatggc aaccgttcac ccaggcacag caacatctgc gtccgccatc
    21421 gcttcgccgc tggattcgac gcaggcggtc gtctgcccct tttgccaaac cctcacccct
    21481 tgcgcgcctg ttgccggtgt ccccctacat caatgtccct gtccctcctg caacgaaaat
    21541 ctgctgattc tcaacccgga cgggcaacca gccgatgagg acatggtcac cccatcgtcg
    21601 gccggcgggt ctgatgccac cgacatgtca cggcgtctcg taggtctcgc tgtggctctg
    21661 ctggcctgtg tcttgaacta tgcactgctg gccggcatgg cgtcctggat gttcaatcag
    21721 atcaaatcga cgcatgatcc ctatgacatc gatgaattga cgctctcccc gattatcttg
    21781 aaagccaacg gttggacagc gcttcacctc gcagcggcac gaggagatgt ctctgaggtg
    21841 acgtcgctgc tggaagacgg gagcccggtc gaccgacaca ataacaatgg acgaacggcc
    21901 ttgttcgaag cagcaacgcg cgggcagaca ccggtggtga cagtgctgct gcaatatgga
    21961 gccaacccca acgcgaggat gaaacaaaac gtgaccgcgc tgctgacggc ggctgagcac
    22021 ggacatgccg acaccattgc cgtgctcctg tccaacggcg ccgagatcaa tgcgctgtgc
    22081 acttgcggtg attcggcgct gcaccgggcc gtacggaaag ggcatctggc cgccgctcaa
    22141 gtcctgctgg aacagggcat cgccgcaaac caaaagagcc atggagagac cgcccttgag
    22201 atcgcgcagc atgacgagga tcgggaattg atcgagctgc tgcgcgcgca cggaggccga
    22261 gaattctccc aggccaaggc ccatcgtgcc caaggcgtgg ccttccagaa gaagggacag
    22321 gtcgataagg cgctcttcgc gtacgccgaa gccctgaacc tggacccgga tgatccggaa
    22381 gcctatttcg accgcggcac cgcgctgctc gaaaaggatt cccccgatga agcactcatc
    22441 gcctttcacg ccgcgatccg cctcaattca accttcatcg aagcctatag cgccgcggcc
    22501 tcggtacacg tcgcgcgccg acagtgggac caggcctcag ccttgtggga ccgatacctg
    22561 gcacaacggc cacaggacgg ccgcgcccat tttgaacgag cgatcgtcaa acggtcgaag
    22621 ggggacagca aaggtttctt gcaggaactg caacaggcct gcaccttcgg gtatcgggcg
    22681 gcctgctgaa ccatgaccac caccgacgcg cagagcgaga ccgccgacca ctccccaagc
    22741 gtcacctgca atcgctgcct cggtcgctat cggattcgcg acgcacgtcg attgacgggc
    22801 agatcccgaa gcctctgccc gacttgcggg gcacgattcg ccgtcgtggt cgccgcgcct
    22861 gctgtaaatc aacggcaaat tgaaggacag gtcccttcgc cggtgctcct ccatcctccg
    22921 gcaaccgacg cccctgaatc caccccctca atgcaacgcg ggtcctttca cggagccggc
    22981 ggcaccttgc tgggcatgca gatcgtcaac gtctgcctga ccatcgtcac ccttggcgcc
    23041 taccactttt gggccaaggc gaagatccgc cggtacctgt tcagtcaaac cgcattcgcg
    23101 ggagaccggt ttgcctatca cggcaccgga aaagaacttt accaggggtt cctgaaagcg
    23161 acggtggtct tcggcattcc ctatttttcg ttaggcgccg cgcatagctt tctggccctc
    23221 ccgcaatcgg tcgatttcct attacaagcg atggctgcgc tggtactgtt cctctatgtc
    23281 ccggtcgcca tcgtcaatgc gcggcggtat cgctgcacgc gcacctcctg gcgaggcatc
    23341 cgattctcgt ttcgaggacg aacggtggac ttcctgaagc tctatttcaa agggtggctc
    23401 ttcaccctcc tgaccctggg cacctactat ccctatttcc agacgcagcg gcaggcattt
    23461 ctccactccc acacctattt cggcaatcag cggttccggt ttaccggaca cggatcgggc
    23521 ctgatggtgc cctttgccgt cacactcttc accacctatg cggtactgtg cctgtgcggc
    23581 ctcgccctgg ccctccagtt gacgaacgcc gggctcacgc tgctcctgat tcccttcgtg
    23641 ctcggtccgg tgtgggtgtg gctactcggg caaaaacaga agtactactg ggatcacacc
    23701 acgttcggcg aggcacgatt ttcatccagc atcacctggc aaaaactgtt cggcctctat
    23761 ctcggcaacc ttgccctctt gctgctcacg ttggggtggg cctggccatg ggtgaccgtc
    23821 cggaacgccc gcttcttcac cggcacgctt tcgttgcagg gtgtgacgga tctcgatcgc
    23881 gtcctgcaag acaccaccga aacatccgtc accggcgaag gcctgtcgaa cctgctcgac
    23941 accggcttcg atatggacta gcccgcatgc ccaccagccg gagcgcccac tatctcgacg
    24001 gtcgcacggc cacgcgccat cgtgtcaccc tcacagtcac ccccgcgacc ctacacattg
    24061 cgatgcccga cggcagtgcc aaacaatggc cctatgacga gatccgccag acgcaaggga
    24121 cttaccgcgg cgaaccggtc cgcctggaat tcgggcctga accggccgaa gccatcgtga
    24181 tttccacagc cgcgttgctg gatgacattc atgaggcggc gccggctctc gcacggcact
    24241 tccacaaccc ggccagacga cacgcgcgca tcggttggac cctctgtgcc gcagtcggcg
    24301 tcgtcctcat ggtcgtgggc ctctatcgat ggggcatccc cggtatcgcc tccgccgcga
    24361 cgccctatat acccgtggcg tgggaagaat ccctgggccg gcaggtcgtc gcacaccttg
    24421 caccggaaac acagcagtgt cgcgatccgg agcgattgcg caaactcgac cacatcgtgc
    24481 aaactctggc cgccactcat cctgcgtccc cctatcggat caccctttcc gtcgtcgatg
    24541 ccccgaccgt caacgcattc gccgcgcccg gcggccggct ggtgattctc cgcgggctgc
    24601 tggaacagac ggccagcccc gaacaactgg ccggagtgct cgcccacgaa ctccaacatg
    24661 tctatcaacg ccatacgacc aaagccattc ttgaacagac agccgccacg ttgctgctga
    24721 ccgccgtctc cggcgacatg tccggcgggc tcgcctgggg cctcgaaggc gcgcgcacga
    24781 tgggctcatt gcactacacc cgcaaccatg aaacggaagc cgatatcgaa ggacttcgta
    24841 tgatgcaggc cgcccgcctc gatcccgccg ccatgattgc cttctacgga accatggaga
    24901 gaggggcgca ggaccatgcc gggccgcctg attttctctc cacccatccg gacatggggg
    24961 agcggctagc cacacttatc gcgctggccg gcccgtcccc gtccgacgca caacggctcc
    25021 tccccggaga agactggaaa gatatccgta cactctgccg cctacaggca ggcgggc
//
LOCUS       DAOQRM010000020        24280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1327_length_24280_cov_14.760826, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000020 DAOQRM010000000
VERSION     DAOQRM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24280
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1327_length_24280_cov_14.760826"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..121)
                     /locus_tag="PKK81_03320"
     tRNA            complement(46..121)
                     /locus_tag="PKK81_03320"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:Asn,seq:gtt)
     gene            complement(186..455)
                     /locus_tag="PKK81_03325"
     CDS             complement(186..455)
                     /locus_tag="PKK81_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31930.1"
                     /translation="MEREDNVLDELLRDIAGLLNEYPKAIERQAAAIHAGGKDPELAE
                     KLIKAADTMRDSGNLYLTWAKHFAALAEGNTDASSDEDETDDFGI"
     gene            complement(533..811)
                     /locus_tag="PKK81_03330"
     CDS             complement(533..811)
                     /locus_tag="PKK81_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNV31931.1"
                     /translation="MYNVAQVIDEKCVAKKGCRLCIMYCPEANCLDLNSTKMVAEVTI
                     DRCKGCELCVVVCNAAKHEAIEMQAVSATGQLMSHKGESAGLGQAYQG"
     gene            complement(835..1542)
                     /locus_tag="PKK81_03335"
     CDS             complement(835..1542)
                     /locus_tag="PKK81_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV31932.1"
                     /translation="MIKKRLNIRMSGLGGQGAVTAAHVMAMAANRDGKFSISNPFFGA
                     EKRMAPAESYCRIGIERIYDRGELVFPDVIEVFHPQVITMGKSYTMPFYSGVKEGGVV
                     IINSAQPLLSEEDINRLKDLNVALFYIAGTELAIEVAGTELSTNMSMIGSVAGITKCV
                     SMEALDGALQERFGKKFVASGGTASLDEAIKKKFAKKEMLLAKNLATVKRAYEIASEW
                     ADKNKVELRVGNPAVAA"
     gene            complement(1560..2462)
                     /locus_tag="PKK81_03340"
     CDS             complement(1560..2462)
                     /locus_tag="PKK81_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV31933.1"
                     /translation="MSKERIKISPDLYDIMPSDYQDLVQSATYGKEDRGWKDIGTSKE
                     LIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIASGLKRALSVRFPDRVKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYVVNCTVSKPSLVEKV
                     IRNSVLIAREIGPTYIQLYTPCILEIGKNSMEGLQEMRDSEKPTERFAYKEYVSEPAK
                     QFLAELAAKDKERKAAAKQLAGKA"
     gene            complement(2532..3743)
                     /locus_tag="PKK81_03345"
     CDS             complement(2532..3743)
                     /locus_tag="PKK81_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNV31934.1"
                     /translation="MAEIKSHIGTQNKKGQTYTDAWEMMHEAPRNPSFYTGSEVIKEA
                     LRRASCDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTIEISYLLNTGML
                     VWHAETAQDFFDWILKGFMVSEEPEVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPY
                     DPYRSPVPCMDMECPPVRMMRDPFIMKSNYISYATHASWQQEIWAAVERSRKHTIKWI
                     NGLIDTENTDAEVVIVASGTAVSQGREAIRLLEDEGVRCGLVKVKSLRPWPGEEIREA
                     TKNAKHIFVPEFNVTGWLAKEIKATIPNSERVHAGPHVCGGMTMPPEIIASEIKAALG
                     LRSESLAGRGS"
     gene            complement(3786..4421)
                     /locus_tag="PKK81_03350"
     CDS             complement(3786..4421)
                     /locus_tag="PKK81_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HNV31935.1"
                     /translation="MTQYRVLPGPEHFLPPAAASMGIYLPNPGEAHINGVIVPEEKAY
                     EEAARQFLMAQVPTIFPGPLVLWAWNEKAAKKAAAVRKLYEILKECVQPGQKPMLIPM
                     PDYRPKYPKINPEVEINPNHPNLTIWHNKIDCCMFIGVHCHQANLSLKIIRGGTSCYT
                     VAMCAQAGHEDAMLSFRDASVEKILKLGEWIRKLKGTVKPRLTTAKTSASN"
     gene            complement(4755..5042)
                     /locus_tag="PKK81_03355"
     CDS             complement(4755..5042)
                     /locus_tag="PKK81_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNV31936.1"
                     /translation="MTFVILGFDGPDGQAKRKIHRTAHLTGLEPLNRAGRVLLAGPFT
                     DQAGSLIIIEADSLEEAKRIAQEDPYTVHGIFERVEVHPFQQVLPASQSHT"
     gene            complement(5061..5474)
                     /locus_tag="PKK81_03360"
     CDS             complement(5061..5474)
                     /locus_tag="PKK81_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HNV31937.1"
                     /translation="MAKSDNDTPDRIFTLAEANELLPQLNQRFASVRRAKTVIASTKE
                     EVSKASAQAASGGGSPVGALYIRALHEISGSLQAIHELGVVIKDVDLGLCDFPYKLEG
                     RVVYLCWKFGEDEIRWWHETSCGYKDRHPLDETSS"
     gene            5987..7234
                     /gene="rho"
                     /locus_tag="PKK81_03365"
     CDS             5987..7234
                     /gene="rho"
                     /locus_tag="PKK81_03365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNV31938.1"
                     /translation="MHLAELKQKSIADLNEVARDLKIEGAANLRKQELIFAILQAQTE
                     KNGVVFGEGVLETLPDGFGFLRAPDSNYLPGPDDIYISPSQIRRFNLRTGDIVSGQIR
                     PPKESERYFALLKVEKVNYEDPEVARDKILFDNLTPLYPEERLNLEFDREEYCTRVMD
                     LTTPIGKGQRGLIVAAPRTGKTMLLQAIARAILKNHKEVTLIVLLIDERPEEVTDWQR
                     QVKAEVISSTFDEPAQRHAQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNTIAP
                     PSGKVLSGGLDSNALQRPKRFFGAARNIENGGSLTIMATALIDTGSRMDDVIFEEFKG
                     TGNMEVHLDRRLADKRLFPAIDISQSGTRKEELLVDRDRLNKMWILRKVLSPLGTMEA
                     MEFLMDKIGGTKTNQEFLQSMNR"
     gene            7335..7541
                     /gene="rpmE"
                     /locus_tag="PKK81_03370"
     CDS             7335..7541
                     /gene="rpmE"
                     /locus_tag="PKK81_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNV31939.1"
                     /translation="MQKGIHPVYREATVHCACGNTFKTRSTIGNIKVDICAGCHPFFT
                     GTQKIVDTEGRVERFKKKYAKKDK"
     gene            7637..8719
                     /gene="prfA"
                     /locus_tag="PKK81_03375"
     CDS             7637..8719
                     /gene="prfA"
                     /locus_tag="PKK81_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNV31940.1"
                     /translation="MEDGLIKKWEGMAARFQELTNQLMDPSVLNQPGMIHKLNKERTE
                     LEEPALQFVAYCEAKKQLDEVSAMLQDASTGADMKQLFAEESDHLQQQVLDLEHRAKD
                     FLTPKDPRDEKNMFLEIRAGTGGDEAALFAGDLFRMYLKYAERNRLKLEVVDASETGI
                     GGYREVIALLEGKGAYGRLRYESGVHRVQRVPTTEASGRIHTSTVTVAVMPEVDEVEV
                     HIDPKDLRIDTFCSSGAGGQSVNTTYSAVRITHIPTGVVVSCQDERSQLKNRTKAMRN
                     LRARIVEAEREKQDAEIAQNRKSQVGTGDRSEKIRTYNFPQNRVTDHRIGLTVHKLDQ
                     VLAGDLEEIVQALRAHYEHVASLEVK"
     gene            8716..9681
                     /gene="prmC"
                     /locus_tag="PKK81_03380"
     CDS             8716..9681
                     /gene="prmC"
                     /locus_tag="PKK81_03380"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNV31941.1"
                     /translation="MSTEQLGIAATSAVTIRSMLAEAERSLHAAGIEQPALEAAWLLE
                     HVLHLSPLMQRVKAERPVPALDYARVLALVARRANREPLQYLLGTQEFCGREFRVTSA
                     VLIPRPESVLLVREAIRRCGQNPAAIVVDVGTGSGCLAVSVASALPDARVLAIDASAD
                     ALAVAQANMEQHGLDARIECMQGDLLAPLAECGFVSQIDVIVSNPPYIPDLDLVTLQP
                     EVRCFEPHLALAGGPDGMDVHRRLLQQAPVYLKSDGVLLLEVGLGQAAAVCREAEESG
                     WFRTYDVLRDEGGIDRVVCFEKKEEGRNPQSGQPSIQTATSRPSS"
     gene            9687..10943
                     /gene="murA"
                     /locus_tag="PKK81_03385"
     CDS             9687..10943
                     /gene="murA"
                     /locus_tag="PKK81_03385"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNV31942.1"
                     /translation="MDQIIIQGGRPLRGEVRTSGAKNAALPILASTILGGGECVLSNM
                     PRVVDVLTMGKLLGMLGISVAQEVNHTVVQAQAIASTEAPYDLVRTMRASVLVLGPLV
                     ARLGEAKVSLPGGCAIGSRPVNFHLAGLEKMGATVEIEHGYIKATARRLRGARIYFDT
                     PSVTGTENLMMAAVLAEGTTVLENAAKEPEITDLAAFLIKRGARITGAGTDMITIEGV
                     TELHGADHAVIPDRIEAGTYLIAGAITGGEVVIDCCRPEHMEPLLMKLRESGVELIEE
                     KERIRLKVTGRLKGTNVRTSPHPGFPTDMQAQFVALMTVAEGTSVVTETIFESRFMHV
                     EELRRMGADIRVEGNRVIITGKERLTGAPVMASDLRASAGLIVAGLAAEGTTEVSRVY
                     HLDRGYERLEEKFRALGASVERRKGK"
     gene            10946..11641
                     /gene="hisG"
                     /locus_tag="PKK81_03390"
     CDS             10946..11641
                     /gene="hisG"
                     /locus_tag="PKK81_03390"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNV31943.1"
                     /translation="MGATKSHSKKSRGLTIALSKGKLLGPVLHLLKQAGYYSPGLSTE
                     SRKLVFACSSNDATFLIVRPTDVPTYVEHGAADVGVVGKDVLLEQGSDVYEPLDLKVG
                     ACRISVAALQGQPSPDRWSSKIRVATKYPNVTERYFNQRGVPVEIVKLYGSIELAPIV
                     GLADRIVDLVETGSTLKAHDLAEVEVITHSTARLIVNRASLKLKHEPLTVLIERLRAA
                     VAVEDSNGAASRA"
     gene            11692..12975
                     /gene="hisD"
                     /locus_tag="PKK81_03395"
     CDS             11692..12975
                     /gene="hisD"
                     /locus_tag="PKK81_03395"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNV31944.1"
                     /translation="MTILASTDRAYDKALRKIVTRARTQGAGVEKSVRTILKAVERGG
                     DRAVLRYTKKFDRLSLKLDHMRVTPEEIKEAYYHIRKDEGDSLRYAAERVRQFHERQR
                     TKTWMYQEQNATLGQMVTPLDAVGVYVPGGKAVYPSSVLMCAIPAKVAGVRRIVMCTP
                     TPKGEINPYLLVAADIAGVDEIYRVGGVQAVGAMAFGTKTIAKVDKIVGPGNIFVATA
                     KRLLYGTVGIDMVAGPSELLVVADDDAKPAHVAADLLCEAEHDEDAQVYLVTTSASLA
                     KEVVRLIQVQLKGLQREKIAAKSIARHFVAFVVPTMDEAIEVANAIAPEHLTLSVDRP
                     FDYLEQVRHAGALFLGRYTPPAVADYVAGPNHVLPTGATARFFSPLSVSDYVKVSNIV
                     HYTKEELTKAKDHIVRLAHIEGFDAHAKSAQSRFA"
     gene            12972..13577
                     /gene="hisB"
                     /locus_tag="PKK81_03400"
     CDS             12972..13577
                     /gene="hisB"
                     /locus_tag="PKK81_03400"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNV31945.1"
                     /translation="MKKNGAPRQASLHRATKETDIRVDWSLDGTGQGTVDTSIRFFDH
                     MLDLLAKHGFFDLTVQAKGDIEIDEHHTVEDVGIVMGKVLHQALGEKAGIKRFGFASV
                     PLDETLAQVTVDLSGRPYLVYNVNLPDRKIKTFDLGLFEDFFQAFVTHGGLNLHVNLQ
                     YGRNPHHIMEAIFKALARALDQATTLEERLSGTVLSTKGSL"
     gene            13605..14273
                     /gene="hisH"
                     /locus_tag="PKK81_03405"
     CDS             13605..14273
                     /gene="hisH"
                     /locus_tag="PKK81_03405"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNV31946.1"
                     /translation="MSAVLSVVHQVPTGRLRTQYMIAIIDYGMGNLRSVSKAFEAMGH
                     QAVVTREARVIADASHVVLPGVGAFGDCMANLERYELIAPIHAAVQSGKPFLGICLGL
                     QLLFTESEEFGVHAGLGIIPGRVKKFVLEPPLKVPHMGWNEIQIVQSAPPFAGLATGS
                     YCYFVHSYYVEPVESSVIATVTEYGRSFASAVWKDNVVACQFHPEKSQAVGLQVIKNF
                     GAWT"
     gene            14306..15004
                     /locus_tag="PKK81_03410"
     CDS             14306..15004
                     /locus_tag="PKK81_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31947.1"
                     /translation="MTLLLLAGCSGTKVTSKASSQVGKYPVRTIALIPFETMATPQAI
                     EASGPAFPVPSGARRSDMAVGVPPTTDQAVRQTHRVPASAGEKVTDLMWAKLKARSGL
                     QLLSPNESARVAREVSGGTATETMPAHKIAQRLGADAALSGKVLVYQERVGSRLGADP
                     PAAVGFEVKLVAPDGIVLWEGNYYEKQRPMTEDFLGFVQRYGVFVTAEELAAYGAEEL
                     ADAFPYGGAAGGGK"
     gene            15027..15752
                     /gene="hisA"
                     /locus_tag="PKK81_03415"
     CDS             15027..15752
                     /gene="hisA"
                     /locus_tag="PKK81_03415"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNV31948.1"
                     /translation="MRVIPAIDLKDGRCVRLRQGDMAAETVYSEDVPSVAGRWQQQGA
                     DLIHVVDLNGAVDGEPKNLPQIEAVMKTVSVKVQVGGGIRTIETVRRYLHAGVARVVL
                     GTAALMDRTFLAQACQEFPRRILLGLDARDGKVAVKGWTAVSETKAIDLLKELAGLEL
                     GAVIYTDIARDGMLNGPNLTALREVVDCSSFPVIASGGITRVEDLQAVHALGPRVEGA
                     IVGKALYDGKLDYAAAVAAIGKR"
     gene            15820..16590
                     /gene="hisF"
                     /locus_tag="PKK81_03420"
     CDS             15820..16590
                     /gene="hisF"
                     /locus_tag="PKK81_03420"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNV31949.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVEVAAIYDREGA
                     DELCFLDITASHENRKTIIDVVEQTAARVFMPLTVGGGVRTLDDIRTLLNAGADKVSI
                     NTTAVQQPEFVREAAQRFGTQCIVVAIDAKQSGQAGRWEVFTHGGRKPTGLDAVEWAQ
                     RMEGYGAGEILLTSMDQDGRQNGYDLALTAAVSERLSIPVIASGGVGTLEHLYDGLVK
                     GKADAVLAASIFHYRTHTIQQAKAYLRDRGVPVRLGPA"
     gene            16606..17289
                     /gene="hisIE"
                     /locus_tag="PKK81_03425"
     CDS             16606..17289
                     /gene="hisIE"
                     /locus_tag="PKK81_03425"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNV31950.1"
                     /translation="MSKESRTMTFDGQGLIPAVIQDWLDGTVLMVGYMNQEAIARTLQ
                     TRSVHFWSRSRRKLWEKGETSGHFLKVKDLFVDCDRDTILVKAEPVGPTCHTGERACF
                     FARMTEEGQAGEEKTQDAGGGILDRVYQTILTRKASPQPDSYVSKLLQGGADRILKKV
                     AEEAGEVIIAAKNQKREEIIYETADLLFHTLLVLGYHDVTLNEVYRELGTRFGKSGIR
                     PSPEGGSRG"
     gene            17282..17626
                     /locus_tag="PKK81_03430"
     CDS             17282..17626
                     /locus_tag="PKK81_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNV31951.1"
                     /translation="MDNCIFCRIVEGGIPAKIVYQDDQVLAFDDINAQAPVHILVIPK
                     RHVAAVQDCREGDQALLGHLLLTCSKIAGMKNLAESGYRIVTNTGAESGQTVFHLHLH
                     VLGGRHMAWPPG"
     gene            17758..19602
                     /gene="dnaG"
                     /locus_tag="PKK81_03435"
     CDS             17758..19602
                     /gene="dnaG"
                     /locus_tag="PKK81_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNV31952.1"
                     /translation="MGQGLISDDVINQIRDRVDIAEIVGQHVALTRAGQNLKGLCPFH
                     QEKSPSFTVSSSRQIFHCFGCGAGGNVYTFLMKLTGAGFPEIVRDLGRKVGVDVPESA
                     GGYSSEARTQLGRLERLNAAAGAYYQRMLLDNTAGAPARAYLDGRGIQHNTMAQFQIG
                     YALPEWDGLTKAMLKEGFAPADLVAAGLSIPRDQSGQQKAAVSGHYDRFRARVMFPIM
                     DLRKRVVAFGGRILGEGMPKYLNSPETPLFKKGQTLFALEAAREAAGQQHTLIIVEGY
                     FDAVALHQAGIRHVAATLGTALTAEHITVIRRFASNVVLLFDPDQAGVRAALRTLDLF
                     VNSGLSVKVVSLPEGDDPDTYVRKYGAEGFTALHEAAPSLLDFAVEHSLRTAESGTVE
                     DRIRAVDEVLRILQKSAHPIEREERIRLVAERLGLSQQRLIDRYPTLASEETRRPSSR
                     PTALPAAAASKPKGNPEERDLAHLLVQGSLSAEDLRKLSPDAFSAPAYRRLIECAMQH
                     LEQDGRVSVRGLLDALINDENCGSLVSELSMLEQHYDDVPAHIAGCLETLERRGRERT
                     MGALIQELKAAERERREADVHRLNGLINEMRIRKAGVMPAALTSAVKE"
     gene            19605..21410
                     /gene="rpoD"
                     /locus_tag="PKK81_03440"
     CDS             19605..21410
                     /gene="rpoD"
                     /locus_tag="PKK81_03440"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNV31953.1"
                     /translation="MPKQELLGEVKKLINLGKEKGFLTYDDLNSTLPADVVSSDQFST
                     IMTMFGEMDIEIVESAETERAPKAAEGEEAIEESEEAEASEENEKEIDLTPGALSRTD
                     DPVRLYLKEMGSVALLSREGEIEIAKRIEEGKKDIATVVYGLPMTLEFVLNLRDQLKN
                     GKIDVREIVPVVETEEDFEEEAVEKDYEELRVKTLDSLNAVRKVSASLKDLYDKARHV
                     GADPEKQKKLRKQIDTVRGQVVDKMESVNLHGVLKDRMTQRVRDLNLLFRQAEREVTS
                     CQRRLGVGGEAGAELLRRLCRTHKDLLAVKRRTSLSEEVLQDIKKHYQAAKGRIRQLE
                     TEEALISAEEIKDAVKHLDVAEEKVKRGKAELVEANLRLVVSIAKKYTNRGLQFLDLI
                     QEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKL
                     IRTSRHLVQKLGREPTPEEIAERMDLPLDKVRKILKIAREPISLETPIGEEEDSHLGD
                     FIEDKKAVSPLEAAIRYDLQRQINGALETLTPREEKVLRKRFGIGEATDHTLEEVGQD
                     FEVTRERIRQIEAKALRKLRHPSRSKKLRSFVESL"
     gene            21565..21641
                     /locus_tag="PKK81_03445"
     tRNA            21565..21641
                     /locus_tag="PKK81_03445"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21599..21601,aa:Ile,seq:cat)
     gene            21719..22495
                     /locus_tag="PKK81_03450"
     CDS             21719..22495
                     /locus_tag="PKK81_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNV31954.1"
                     /translation="MNLQLSPLIELQKLDLRIADLKEQRRKIPERLEENEAPLREAKR
                     LFREASASVESLVKDRRGHEKDLEAHEDRIGKMKDRAAQLKTNQEYQAHLFEVELANK
                     KRGEIEEKILLAMEQIEQTQRTIVDVQAKLKESEALFIKEKAKLDEKDRLLATELAEL
                     EVKQKHLSGQVEKSLLARYNKLKTTRKEQALALIKDGICVGCRLQLPPQLVSQVKRGE
                     DVHTCPYCYRMLYWEGDPATEAKGTVDHDQAKNLEVGESF"
     gene            complement(22492..23151)
                     /gene="rnhC"
                     /locus_tag="PKK81_03455"
     CDS             complement(22492..23151)
                     /gene="rnhC"
                     /locus_tag="PKK81_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HNV31955.1"
                     /translation="MPAATSLSSLDRIGIDESGKGDYFGPLVIAAVFVTPALEQDLAL
                     MQVRDSKKISDGRILELAPDIRLLCPHSLVAIGPQRYNELYAKIKNLNRLLAWGHARA
                     LENLLQQVECDLAIADQFGDERLILNALQVKGKQIRLVQRTKAESDLAVAAASILARA
                     EFLQRLDRLSQELNTTLPKGASPAVELAGRMVVKKYGRDRLGTVAKLHFKTTKQVLGE
                     T"
     gene            23238..24128
                     /locus_tag="PKK81_03460"
     CDS             23238..24128
                     /locus_tag="PKK81_03460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV31956.1"
                     /translation="MEAQQKMVRRRTSMMRSNMLCLAGVVVAMLVTTGCQTNPYTGRW
                     QLMMMPMSQETQMGAQAYAEVKNDPKMKTSTDPREIEPVKRVAARVIEAAKRSKYSEM
                     ANQFEWEVTVIKDDKTMNAFALPGGKIAVYTGIFPVAKTEAGLAAVMGHEVVHALARH
                     GGERMSQNTLAQTTLQAIGIALGVSGANPVVSQGAMAALGVGAQVGVLLPFSRKHESE
                     ADYVGVLLAADAGYDPRESIQLWTRMGELSGGKSASEFMSTHPSHETRIEQLEEWMSE
                     AMPIYQSKPPAPNHELPALR"
BASE COUNT         5225 a         6333 c         7501 g         5221 t
ORIGIN      
        1 agagaagggg ctggcggtgt tattccgtca gccccctttg tattgtggct ccccgggcag
       61 gactcgaacc tgcgaccagc cggttaacag ccggctgctc taccaactga gctaccgggg
      121 aacaaagaga ttcaagataa tcgtcggcat tctagcaaaa ctaaagagag aagggggaga
      181 cttttttaga tgccgaaatc atcagtctcg tcttcgtctg atgaggcgtc cgtatttcct
      241 tccgccagcg cggcaaaatg ttttgcccag gtgaggtaga gattcccgct atcccgcatc
      301 gtatcggcgg ctttgatgag tttctcggct aattcagggt cttttcctcc ggcgtgtatg
      361 gctgcggctt gccgctcaat cgccttggga tattcattca gcaggccggc gatatcacga
      421 agcagttcat cgagcacatt gtcttcacgt tccatgggat cctctcagtg ctcagttcaa
      481 gaaaaggccc catagcgtgg aacgctatgg ggccttgcga caacgcgact cgctaccctt
      541 ggtaggcttg cccgaggcct gcggactcgc ccttgtggct catcagctgc ccagtggcgc
      601 tgactgcctg catctcaatc gcttcgtgtt tggcggcgtt gcagacaacg acacaaagtt
      661 cgcatccctt acaacgatca attgtgactt ccgccaccat tttggtcgag ttaaggtcca
      721 agcagttggc ttccgggcaa tacatgatgc agaggcggca gcccttcttg gccacgcact
      781 tctcgtcgat cacttgcgct acgttataca tgcgacgtgg ttccttctct ctcgttaagc
      841 tgcaacggca ggattgccaa cccgcaactc gaccttgttc ttatcagccc attcgcttgc
      901 gatttcgtag gctcgcttga ctgttgccag gttcttcgcc aatagcattt ctttcttggc
      961 gaacttcttc ttgatcgctt catccaagga ggcggtacca ccggatgcca cgaacttctt
     1021 gccgaatcgt tcttgcaagg cgccgtcgag agcttccatt gagacgcatt tggtaatacc
     1081 tgcgaccgaa ccgatcatcg acatattcgt cgagagctcg gtaccggcga cttcgatcgc
     1141 gagctcggtt cccgcaatgt agaacaaggc cacgttcagg tccttgaggc gattgatatc
     1201 ttcttctgac aagagaggct gggccgagtt gatgatgaca acccccccct ctttgacacc
     1261 ggagtagaag ggcatggtgt agctcttgcc catcgtaata acttgaggat gaaatacttc
     1321 gatcacatcg ggaaatacca actcaccccg gtcatagatc cgctcaatcc cgatccgaca
     1381 gtaactttct gcgggagcca ttcgtttttc agcgccaaaa aatggattcg aaatggaaaa
     1441 ctttccgtct cggttggcgg ccatggccat tacgtgggct gccgtcacgg cgccttgccc
     1501 acccaacccg gacatgcgaa tattgagtct cttcttgatc atgagcgacc tccgattcct
     1561 taagcttttc ccgctaactg ttttgcggcg gctttgcgtt ccttgtcctt agcagctagt
     1621 tcagccagga actgcttcgc cggttcgctg acatactctt tataagcaaa tcgctcagtt
     1681 ggcttttcag agtcacgcat ttcctggagg ccttccatgc tgttcttgcc gatttcaagg
     1741 atgcacggcg tgtacagctg gatatacgta ggtccaattt cacgtgcaat gaggacggaa
     1801 ttccgaatca ccttttccac aagtgacggc ttgctcactg tgcagttaac gacgtagtga
     1861 cagccggact ctcgggcaat ttcaggcaaa cggactttgt cgaacagttt tccaaccggg
     1921 gccattttgg caacgaagcc tttttgcatc aggccgctct cctggccacc ggtattggcg
     1981 tacagttcat tgtcgaaaca gatggtcgtg aacttttcct ggcggaacca ggcttgcagg
     2041 gtcatgtcca ggccgatatc gaccgtcgca ccatcgcctg ccaagacgac tacgtctttc
     2101 acgcggtcag ggaatctcac gctcagggct cgcttcagcc cggatgcaat ggcgttttga
     2161 ttgccaaaga gggagtgaat gttgtgcaca gccaccatgg gaaacacgag gctggtgcag
     2221 cctgtggaac cgaccatcac cgtatcctca gggtttggga gtgaggccag gatatagcga
     2281 aaggccatgg attcagggca accggcgcac aaggagtgct gctcaatcag ttctttagag
     2341 gttccaatat ctttccagcc acggtcctct ttcccatagg tcgcgctttg aacgaggtcc
     2401 tgatagtccg acggcatgat gtcatatagg tccggcgaaa ttttgattcg ctctttgctc
     2461 atgtatgcct cctcaaggct tgtctatcac gtaatgatgg ctcaatggac ggtgtcacaa
     2521 ctcagctact atcagctacc tcttccagcg agtgactcgg agcgcaagcc tagagccgct
     2581 ttgatctctg aggcaataat ttctggcggc attgtcattc cgccacacac atgcgggccg
     2641 gcatgaacac gctcgctgtt agggatggtg gctttgattt ctttcgccaa ccagcccgtc
     2701 acgttaaact cgggcacaaa aatgtgtttc gcattcttgg tggcctcgcg aatttcctct
     2761 ccgggccaag gacggagtga ctttaccttc accagtccgc aacgcacgcc ttcgtcttcg
     2821 agcaggcgaa tagcttctcg cccctgcgag accgcagtgc ctgacgcaac aatcacgacc
     2881 tcagcatcgg tgttttccgt atcgatcagg ccattgatcc acttgatggt gtgtttgcgc
     2941 gagcgttcaa ccgccgccca aatttcttgt tgccatgaag cgtgggtggc ataactgatg
     3001 tagttgctct tcataatgaa cgggtcacgc atcattctga ccggcggaca ctccatgtcc
     3061 atgcaaggca cgggtgagcg gtagggatcg taaggtggta ggcacatatc tgctggagtg
     3121 aggttcacga cgtctttcgt atgggtcacg aagaagccgt cgcagcatag cgccagaggt
     3181 aaatgcactt ccggctcttc cgataccatg aagcctttga ggatccaatc gaaaaagtcc
     3241 tgtgccgttt ctgcatgcca aaccagcatg cctgtattta acaggtagga aatctcaatg
     3301 gtatccggct gaatcgacaa cggggagttg atgccgcgac acgtgacgat catttgaatc
     3361 ggcagacgcg cacctgccca cataggaaag ttctccatcg cgcgcattgt gccgggtcct
     3421 gcggtcgtag tgaacacccg tgcgccacca aaggcagcgc cggcgcattg ggacataacg
     3481 gcaaactcgc tctctcctcg gaaatagtcg ccgatatagc cttcagcaaa taattcgccg
     3541 atgagggcag cagcttcgct ttgaggggtg atgggatagg caatcatcac gtcgcagctt
     3601 gcacgccgca aggcttcttt aataacctcg ctgccggtat aaaaggacgg gttgcgaggg
     3661 gcctcgtgca tcatctccca cgcatctgta tacgtctggc cctttttatt ctgtgtcccg
     3721 atgtgggatt ttatttccgc catggactcg gcctcctttt aatcgttaga agtgcttgaa
     3781 gccacttagt ttgacgcgct ggtctttgct gtggtcagcc gcggtttgac cgtgcccttg
     3841 agtttacgga tccattcgcc gagcttgagt attttctcca cagaggcatc tctaaaggag
     3901 agcatggcat cttcatgtcc tgcctgggcg cacatcgcga cggtatagca ggacgttccg
     3961 ccccggatga tcttaagcga cagattcgcc tggtggcagt gcaccccgat gaacatgcag
     4021 cagtcaatct tgttatgcca gatggtcagg ttcgggtggt tgggattgat ttccacttcg
     4081 ggattgatct tcggatactt gggacgatag tccggcatag gaatcagcat cggtttctgc
     4141 ccgggctgaa cgcactcctt caaaatttcg tacagctttc tgacggcagc ggccttctta
     4201 gcggcctttt cgttccaggc ccacaagact aaggggcccg ggaaaatggt cggtacctgt
     4261 gccatcagga actggcgtgc cgcttcttcg taggcttttt cttcaggaac gatcacgccg
     4321 ttgatatgtg cctcgcctgg attggggaga tagatcccca tggaggctgc tgccggtggc
     4381 aggaagtgct ctgggcctgg taacacgcga tactgagtca ttcgaacctg ctctcttttg
     4441 ggggttaaca acttggagaa gggacaccct cggttaaagc gtggggtcaa gtcactctat
     4501 gctttctcga tgtttctctg caacacggca gaaggcccac tttggggaaa tttagggcca
     4561 gcgctggaga aggccgttag gtcttttgaa ctagagtgat tcggctttca caaacggcgc
     4621 taagtgcgca ttataggtgg cgatctttcg ggttgtcaat ctgaggaata tgcgggacgc
     4681 tacccagtgt aagtgggacg taggtgctta ctcttgctgc gagtaactca cctcgggtcc
     4741 ctgaagaacg ttgattacgt gtgcgattgt gaagcgggaa gtacctgttg gaagggatgg
     4801 acttccacgc gttcgaaaat tccgtgcact gtgtaggggt cttcttgggc gatccgcttg
     4861 gcttcttcga gagaatctgc ctctatgatg atcaggctcc ctgcctgatc agtgaacggg
     4921 ccggctaaga gcactcgccc tgcgcggttc aatggctcca gtccggtcag atgagcggtg
     4981 cgatgaattt tgcgttttgc ttggccgtcg ggcccatcaa agccaaggat gacgaatgtc
     5041 atggagggca cagtcctttg ctagctagag gtttcgtcaa gcgggtggcg atctttatat
     5101 ccacaggaag tctcgtgcca ccatcgaatt tcatcctcac cgaacttcca gcacagatag
     5161 accacacgac cttcgagctt atatggaaag tcacatagcc cgagatcgac gtctttgatg
     5221 accacgccga gttcgtgaat cgcctgaagg cttccgctga tctcgtggag ggctcggata
     5281 tagagcgctc caacggggct tccgccgcct gacgcggcct gcgcgctggc tttgctcacc
     5341 tcctccttcg tgctcgcgat aacggttttc gcccggcgta ccgaggcaaa tcgctgattg
     5401 agttgaggga ggagttcgtt ggcttcggcc aacgtaaata tgcgatccgg tgtgtcgttg
     5461 tctgatttgg ccatgaaaat ttccccgcgc gatctttaac atatcactcc acggcctgca
     5521 accgagatgc ccgtccacac ttggggttta cttattaagg agactggtat gtaacggtcg
     5581 gagcgttgtg gggctagtaa gaaggtgtgt tgtggttcga aggacgacgg atcagacgtt
     5641 aatctattga caatgtgcgg ggtcggcgct aaaatcacct caacttcgca atttgtagaa
     5701 attgctttac cttctgtgca tcagcttccg agacccatct caagcataaa ttcccaaggg
     5761 cagtgcagcg gcagttgtgc aatagtgggt tgttttcatt tccgcaaaat ctaaatcgac
     5821 aacaccttca tcgaaccatc ctgccgtaga caaccagcag gtcgacatct agacaatcac
     5881 ttccgtgaat tcgttcatga ccactaaatc gtttttctca ttttggggag ctgtcgggtt
     5941 gagagcccga tagggaagag gtatgacaca ggttaaaggg gaagttatgc atctcgcgga
     6001 actcaagcag aagtcgattg ccgacctgaa tgaggtggcg cgggatctga agattgaggg
     6061 ggcggcaaac ctgcgcaagc aggagctcat ttttgccatt ctccaagcac agacggagaa
     6121 aaacggtgtt gtgtttggcg aaggagtgct ggagaccttg ccggatggtt ttgggtttct
     6181 gcgcgcacct gactcgaact atctccctgg gcctgacgat atctatatct ccccctcaca
     6241 gattcgccga ttcaacctcc ggacgggcga tattgtgtcg gggcaaatac gtccgcccaa
     6301 ggagagcgag cgttatttcg ctttgctcaa ggtggagaag gttaactatg aggatcctga
     6361 agtcgcgcgt gacaagatcc tgttcgataa cctgaccccc ctctatcctg aagagcgcct
     6421 caatctcgaa tttgaccggg aagagtactg cacgcgtgtc atggatctca cgacgccgat
     6481 cggcaagggg cagcgcggcc tgatcgtcgc cgcgccgcga accggaaaaa ccatgctgct
     6541 gcaggcgatc gcccgcgcga ttttgaaaaa tcacaaggaa gtcacgctta ttgtcctgtt
     6601 aattgatgag cgacctgaag aagtcacaga ctggcagcgg caagtgaaag ccgaagtcat
     6661 cagttctaca ttcgacgaac cggcacagcg ccatgctcag gttgcggaaa tggtgctgga
     6721 aaaggcgaag cggctggtgg agcacaagaa ggatgtggtg atcctcctcg atagcatcac
     6781 tcggttggcc agggcataca atacgattgc ccctcccagc ggaaaggttc tctccggcgg
     6841 attggattcc aacgctttgc agcgtccgaa acgattcttc ggcgctgcca gaaacattga
     6901 aaacggcggg agcttgacga tcatggcgac cgcgctgatc gataccggca gccgcatgga
     6961 cgatgtgatc ttcgaagaat tcaagggaac tggtaacatg gaagtacact tggatcgtcg
     7021 cctggccgac aagcgtttgt tcccggcgat cgatatcagt cagtccggga cccgcaagga
     7081 agagttgctg gtcgaccgcg atcgcttgaa caagatgtgg atcctgcgga aggtgttgag
     7141 cccgttgggg acgatggaag ctatggagtt cttaatggac aagatcggcg gcaccaaaac
     7201 caatcaggaa tttttgcaat ccatgaatcg ttaggtgtcg cgcttgccag tgtggggcac
     7261 tccagtgtaa agtcgtgcac cctggcgagc aggaccgggc gagtcaacga atcaagggca
     7321 gtagaggagc tcgtatgcaa aagggaattc atccagtcta ccgtgaagcg actgtgcatt
     7381 gtgcctgcgg gaacacgttc aagacgcgat ccaccatcgg aaacatcaag gtcgacattt
     7441 gtgccgggtg tcatcccttc tttacgggaa ctcagaaaat cgtcgatacc gaagggcgtg
     7501 tcgaacggtt caagaagaag tacgccaaga aagataagta ggcctcagtc aggacatcat
     7561 ggagaccctg cttccttttg gggcagggtc tcttgttttt tggggtaaag cgggctacgg
     7621 ccggaatggg cgggacatgg aagacgggct gatcaagaag tgggaaggca tggctgcgcg
     7681 cttccaagag ttgaccaatc agctcatgga tccgtctgtc cttaatcagc cggggatgat
     7741 ccacaagctg aacaaggagc ggacagagtt ggaagagccg gcgcttcaat ttgtcgccta
     7801 ctgcgaggcg aagaagcagc tggacgaagt atctgccatg ctccaggacg cctcgacagg
     7861 ggcggacatg aagcagctgt ttgcggaaga aagtgaccat ctccagcagc aggtgctgga
     7921 tctcgagcat cgcgctaaag actttctcac tccgaaggat ccgcgcgacg agaagaatat
     7981 gtttctcgaa attcgagctg gaacgggtgg ggatgaggca gccctgtttg ccggagatct
     8041 gttccggatg tatctcaaat atgccgagcg caatcggctc aagttagaag tcgtcgatgc
     8101 gtctgagacc ggaattggtg gttatcgaga ggtgatcgct ctactcgaag ggaagggcgc
     8161 gtacgggcgt ctccggtacg aaagcggagt gcaccgtgtg cagcgggtac cgaccacgga
     8221 agccagtgga agaattcata cctcgacggt gacggttgcg gtcatgccgg aggtggatga
     8281 agtcgaggtg catatcgatc cgaaggattt gcgtatcgac acgttttgtt cgtcaggtgc
     8341 gggcgggcag agtgtcaata cgacctattc ggcggtccgc attacgcaca ttccaaccgg
     8401 tgtggttgtc agctgccagg atgagcggtc gcagctcaag aatcgaacca aagccatgcg
     8461 gaatttgcgg gcgcgcattg tcgaagctga gcgtgaaaaa caggatgcgg aaatcgcaca
     8521 aaaccggaaa tcgcaggtgg gaacgggtga tcgaagcgaa aagatccgca cctacaactt
     8581 ccctcagaac cgtgtgaccg atcatcgcat cggcctgacg gtccacaagc tcgatcaagt
     8641 cttggcgggt gatctggaag agattgttca ggcgctacgc gcgcactatg aacatgtagc
     8701 ctccttggaa gtgaaatgag tacggagcag ctcggcattg ccgccacttc cgcagttacc
     8761 atccgttcca tgctggctga ggctgaacgg agtctgcatg cggccggtat cgagcagccg
     8821 gcattggagg ctgcctggct gttggagcat gtgctgcatc tctcgccgct catgcagcgg
     8881 gtgaaggccg agcgtccggt gccggcgctg gactatgcga gggtgctagc gctcgtcgca
     8941 cggcgggcca accgtgagcc gctgcagtat ctgctgggta cgcaggagtt ttgcgggcgg
     9001 gaattccgtg tcacatccgc tgtccttatt ccacggccgg agagtgtctt attggttcga
     9061 gaggcgatcc gtcggtgcgg gcagaatccc gccgcgatag tggtggatgt gggaaccggg
     9121 tccggttgcc tggctgtgtc tgtggcatcg gccctgcctg acgcgcgagt gctggcgatc
     9181 gatgcctcgg ctgatgcatt ggctgtggcg caggccaata tggaacaaca cggactcgat
     9241 gcacgaattg aatgtatgca gggggatctc cttgcgccgt tggccgagtg tgggttcgtg
     9301 agtcaaattg acgtcattgt gtcgaatcct ccctatatcc ccgacctcga tctggtgacg
     9361 ctccaaccgg aagtgcgatg tttcgagccg catttggcgt tagccggcgg gcccgatgga
     9421 atggatgtgc atcgtcggct tctgcagcaa gctcccgtct atttgaagtc ggatggtgtg
     9481 ctgctgctgg aagtgggcct tggtcaggcg gcggcggtct gccgcgaggc cgaggaaagc
     9541 ggatggtttc gcacctacga tgttctgcgg gacgaaggtg gcattgaccg cgtggtctgt
     9601 ttcgagaaaa aagaagaggg gaggaatcct cagagcggtc aaccaagcat ccaaacggca
     9661 acgagcagac cgtcttctta attctcatgg accaaatcat tatccagggt ggcaggccgt
     9721 tacgagggga agtgcgcacc agcggggcga aaaacgccgc gctgccgatt ctcgcgtcga
     9781 ccattcttgg cggcggcgag tgtgtgcttt cgaatatgcc tcgggtcgtc gatgtactca
     9841 cgatggggaa gttgctcggc atgctgggca tctccgtcgc gcaggaggtg aatcatacgg
     9901 ttgtgcaggc gcaggcgatt gcttccacgg aagcgccgta cgatcttgtt cgaaccatgc
     9961 gggcctccgt cttagtgctg ggacccctgg tggccaggct aggtgaggcg aaggtgtcgc
    10021 ttccaggcgg ttgtgcgatc gggtctcgcc cggtcaactt ccacctcgcg ggcttggaaa
    10081 aaatgggcgc gaccgtcgaa atcgagcacg gctatatcaa ggcgaccgca cgtcgtttga
    10141 gaggagctcg tatttacttc gatacgccca gcgtgaccgg tactgagaac ctcatgatgg
    10201 cagccgtcct ggccgaggga actacggtgc tcgaaaatgc ggcgaaagaa cctgaaatca
    10261 ccgatctggc cgcctttctc atcaaacgcg gcgcgcgcat caccggggcg ggcacggaca
    10321 tgattacgat tgagggtgtg acggagttac atggcgcgga tcatgccgtg attcccgacc
    10381 gaattgaggc gggcacgtat cttatcgccg gcgccattac cggcggtgag gtggtgattg
    10441 attgctgtcg cccggagcac atggagccgt tgttgatgaa actgcgggag agcggcgtgg
    10501 agttgatcga ggaaaaggaa cggattcgcc tcaaggtcac cgggcgcttg aaggggacga
    10561 atgtccggac cagtccgcac cctggttttc ccacggatat gcaggcgcag tttgttgcgc
    10621 tcatgacggt agccgaaggg accagcgtcg tcacggaaac gattttcgag agccggttca
    10681 tgcatgtgga ggagttgcgc cgtatgggag cggatattcg agtcgaagga aaccgggtga
    10741 tcattacggg caaagaacgg ttaacaggtg ctccggtcat ggcctcggat cttcgcgcca
    10801 gcgccggact gatcgtggcc gggctcgccg ccgaggggac gacggaagtc tcgcgggtct
    10861 atcacctcga tcgaggctac gagcgtctcg aagagaagtt cagagcgttg ggcgccagtg
    10921 tagagcggag aaaggggaag tgaccgtggg ggcgacgaaa tcgcacagca agaagtcgcg
    10981 ggggctgacc attgcactgt ccaagggcaa gttgttggga ccggtgctgc acctgctgaa
    11041 gcaagccggc tattatagtc ccggcctgtc gacggagagc cgtaagttgg tgtttgcctg
    11101 ttcgtccaac gatgccacct tcttgatcgt caggccaacc gatgtgccca cctatgttga
    11161 gcacggagcg gcggatgtcg gggtggtggg caaagatgtg ttactggagc agggcagcga
    11221 cgtctatgag ccgttggatt tgaaggtcgg agcgtgtaga atctcggtcg ccgcgctgca
    11281 ggggcagcct tcgccggacc ggtggtcgtc gaagattcgg gttgcgacga agtaccccaa
    11341 cgtaaccgag cggtatttca atcagcgtgg ggtgccggtc gaaattgtga aattgtacgg
    11401 gtctatagag ctcgcgccga tcgtcggatt agccgatcga atcgtcgatt tggttgaaac
    11461 aggcagcacg ctcaaggcgc atgacctggc agaggttgag gtgatcacgc actcgaccgc
    11521 caggctgatt gtgaatcgag ccagcctcaa attgaaacat gaacctctca cggtgttgat
    11581 cgagcggttg cgcgccgcgg tggcggtgga agatagcaac ggggcagcat cgcgcgccta
    11641 agtgtaagta tatgagttcg tggcgggtgt cgaagcggcg agacagatga tatgaccatt
    11701 ctggcaagta ccgatcgggc gtatgacaag gcgttgcgaa aaatcgtgac ccgcgcgcgc
    11761 actcaaggcg caggtgtgga gaagagcgtg cgcacgattc tgaaggccgt ggagcggggc
    11821 ggagatcgtg cagtcctccg gtacacgaag aagttcgatc ggctgtcctt gaagctcgat
    11881 cacatgcgtg taacccccga agagatcaag gaggcctact accacattcg caaggatgag
    11941 ggagactctt tgcgctatgc cgcggaacgt gtacggcaat tccatgagcg tcagcgcacc
    12001 aaaacctgga tgtatcagga acaaaacgcc acgctcggtc aaatggtcac gcccctggac
    12061 gctgtcggtg tgtacgtgcc gggcggcaag gccgtctatc cctcatcggt gctgatgtgc
    12121 gcgattcccg caaaagtcgc cggtgttcgg cgtatcgtca tgtgtacccc gaccccaaaa
    12181 ggggagatca atccctatct gttggtggcc gcggatatcg ctggtgtcga tgaaatctac
    12241 cgtgtgggcg gtgtgcaggc agtcggcgcg atggcgttcg ggacgaagac catcgccaag
    12301 gtcgataaaa ttgtgggtcc cggaaatatt ttcgtcgcca cggccaagcg actactttat
    12361 ggcaccgtcg gtatcgatat ggtggccggt cccagtgagt tgctcgtggt ggcggatgat
    12421 gacgccaagc cggcacatgt ggcggcggat ctgctctgcg aggcggagca tgacgaagat
    12481 gcgcaggtct atctcgtgac gacctccgcg tcgttggcca aagaagtggt gcgcctgatt
    12541 caggtgcagt tgaaaggctt gcaacgagag aaaattgcgg cgaagtcgat tgcccggcat
    12601 tttgtggcat tcgtggttcc taccatggat gaagccattg aggtggcgaa tgcgattgcg
    12661 cccgagcact tgaccctctc ggttgatcgg ccgttcgatt atttggagca ggtgcggcac
    12721 gccggggccc tgttcctggg ccgctacacg ccacctgcgg tcgcggatta cgtcgccggt
    12781 ccgaaccacg tgttgccgac cggggcgacg gcccgcttct tttcgccgtt gtcggtcagc
    12841 gattacgtga aggtgagcaa catcgtgcat tacaccaagg aagagttgac gaaagccaag
    12901 gatcatatcg tccgactggc gcatatcgag ggcttcgatg cgcatgccaa atcagcccaa
    12961 agcaggttcg catgaaaaag aacggcgctc ccaggcaggc ctcgcttcac cgtgccacga
    13021 aggaaacgga tatccgcgtt gactggagtc tggacggcac cgggcagggg actgtcgaca
    13081 cctccatccg gttcttcgac cacatgctcg atcttctcgc caagcatggt ttcttcgacc
    13141 tgacggttca ggccaagggc gatatcgaaa tcgacgaaca ccacaccgta gaggatgtcg
    13201 ggattgtcat ggggaaggtc ctgcaccagg cgttgggtga aaaggcgggg atcaagcgat
    13261 tcggattcgc ctcggtccca ttggatgaaa ccctcgcgca ggtgacggtt gatctgagtg
    13321 ggcgtcccta tctcgtgtac aacgtcaatt tgccggatcg aaagattaag accttcgatc
    13381 tcggcctgtt cgaggatttt tttcaggcct ttgtgaccca cggtgggctg aatttgcatg
    13441 tgaatctcca atacggacgc aatccgcacc acatcatgga agctatcttc aaggcgttgg
    13501 cccgagcgtt ggatcaagcc accacgttgg aagagcgctt gtcagggaca gtcctctcca
    13561 cgaagggaag cctctaagtg aaaccggtgg cgcgatagga ggcagtgtct gccgtcctgt
    13621 ctgtcgtcca tcaagtcccc acgggccgtc tccgaacgca atatatgatt gccatcatcg
    13681 attacggcat gggtaatttg cggagtgtct ccaaggcctt tgaggcgatg gggcatcaag
    13741 cggtggtcac gcgcgaggct cgtgtcattg ctgatgccag tcacgtggtg ttgcccggtg
    13801 tgggggcctt cggggattgc atggcgaatc tggaacggta cgaattaatc gcgccgattc
    13861 acgccgccgt tcaatccgga aaaccttttc taggtatctg cctggggctg caattgctgt
    13921 tcacggagag tgaagagttc ggggtgcacg caggtctcgg catcattcct ggtcgggtga
    13981 agaaatttgt gctggaacct ccgctgaagg tgccacatat ggggtggaac gagattcaaa
    14041 tcgtgcaatc cgcccctccg tttgcaggcc tcgcgaccgg cagttattgt tattttgtgc
    14101 attcctacta cgttgagccg gttgagtctt cggtgatcgc caccgtgacc gagtacgggc
    14161 ggtcctttgc gtccgccgtg tggaaagaca atgtcgtcgc ctgccagttt catcctgaaa
    14221 aaagccaagc tgtcggactt caagtgatca agaacttcgg ggcctggacg tgagagaccg
    14281 gtctgtgcgt gctctattga ttccgatgac gttgctgctt ctggcgggct gcagcgggac
    14341 gaaggtcacg tccaaagctt catcgcaggt gggaaagtat ccggtccgca caatcgccct
    14401 cattccgttt gaaactatgg cgactcccca agcgatcgag gcgtctgggc ccgcgtttcc
    14461 cgtcccgtcc ggcgcaagac ggtccgacat ggctgtcggg gtccctccga cgaccgatca
    14521 agccgtgcgc cagactcatc gcgtaccggc ctccgcaggt gagaaagtca ccgatctgat
    14581 gtgggcaaaa ctcaaggccc gatccgggtt acaactactc tcccctaacg aatcggcaag
    14641 ggttgctcgg gaagtttccg gggggactgc gacggaaacg atgccggccc acaagattgc
    14701 tcaacgactc ggagccgatg cggcactcag cggcaaggtg cttgtctatc aggaacgagt
    14761 cggcagtcgg cttggcgcgg acccaccggc cgccgtggga ttcgaggtga agctggtagc
    14821 gcccgacggc atagtcctgt gggagggcaa ctactatgaa aagcagcggc cgatgacgga
    14881 agacttttta ggctttgtcc agcgctatgg cgtgtttgtc actgccgagg agttggccgc
    14941 ctatggagcg gaggaattgg cagatgcgtt tccatatggc ggcgcagcag gcgggggaaa
    15001 atgaacgtgt tgcgacaggt ggtgtcatgc gtgtaattcc ggcgattgat ctcaaagacg
    15061 gccgctgtgt ccgattgcgg cagggcgaca tggccgctga aacggtgtac tcagaggacg
    15121 tgccttcggt ggccggccgc tggcagcagc aaggcgccga tcttattcat gtcgtcgatc
    15181 tgaacggagc cgtcgatggg gagccgaaga atcttcccca gatcgaagcg gtcatgaaga
    15241 cagtcagcgt gaaggtgcag gtgggaggtg gaatccggac cattgagacc gtgcggcgat
    15301 atctccatgc gggtgtggcg cgcgtggtgc tcggcacggc tgcccttatg gatcgaacgt
    15361 ttctggcgca ggcctgtcag gagttccctc ggcgtatttt gttgggactc gacgcgcgag
    15421 acgggaaggt cgcggtgaag ggctggacgg cggtgtccga aacgaaggcc atcgatctcc
    15481 tcaaagaact tgccggcctt gaactggggg ccgtcattta caccgatatc gcccgggatg
    15541 gaatgctcaa tgggccgaac ctgacggcgt tgcgagaagt cgttgattgt tcgtcgtttc
    15601 ccgtcatcgc gtccggtggc atcacgcgtg tggaagatct gcaggcggtg catgcgctcg
    15661 gtcctcgcgt ggagggggcc atcgtcggca aggccctcta cgacgggaaa ttggattatg
    15721 cggcagcggt tgcagccatc ggaaaacgtt gaggatcttg catgcggctg gacgaaaata
    15781 aaaccggctc gcttcacgag tcacgcttga cgaacctcta tgctgactaa gcgcatcatt
    15841 ccctgtttgg atgtgaagga tggtcgagtc gtcaaaggcg tgagcttcgt gaatttgcgg
    15901 gatgccggtg atccggtcga ggtggctgcc atctatgatc gcgaaggcgc ggacgaattg
    15961 tgtttcctgg atatcaccgc ctcacacgaa aaccgcaaga ccatcatcga tgtagtggag
    16021 caaaccgccg ctcgtgtgtt tatgccgctc acggtcggag gcggggtgcg tacgctggat
    16081 gacattcgga ctttgctcaa tgccggggcc gacaaagtca gcatcaacac cacggcggtg
    16141 cagcagccgg aatttgttcg cgaggctgcc cagcgattcg gtacgcagtg catcgtggtg
    16201 gcgatcgacg ccaaacagtc cggtcaggcg ggtcgctggg aagtgttcac ccacggtgga
    16261 cgaaaaccaa ccggcctgga cgccgtcgag tgggcgcaac ggatggaagg gtatggggcc
    16321 ggagagatcc tcttgaccag catggatcag gatggtcgcc agaacgggta tgatctggcg
    16381 ttgactgcgg cggtttcgga acgtctctcg attccagtga ttgcctcggg cggggtgggg
    16441 accctggagc acctctacga cgggttggtg aaggggaaag cggacgccgt cctggccgcc
    16501 tcgatctttc actatcgaac tcataccatt cagcaagcca aggcatatct gcgagaccgc
    16561 ggggttccgg tgcgactggg accagcctag atggagcaga atgtgatgtc gaaagagagt
    16621 cggaccatga cgtttgatgg acaagggttg attcccgccg tgattcaaga ttggctggat
    16681 ggcaccgtgt tgatggtcgg gtacatgaat caggaggcca ttgccaggac gcttcagacg
    16741 agatccgttc acttttggag ccggtctcgc cggaagttgt gggaaaaggg cgagacgtcc
    16801 gggcatttcc tcaaggtgaa ggatctcttt gtcgattgtg atcgtgacac gattctcgtg
    16861 aaggccgaac ctgtcgggcc gacctgccat accggtgagc gtgcctgctt tttcgcccgg
    16921 atgacggaag aggggcaggc cggtgaggag aagacccagg atgccggtgg ggggatcttg
    16981 gatcgggtgt atcagaccat tctgacgcgt aaggccagtc cgcagcccga ctcctatgtg
    17041 tcgaaattat tgcagggcgg agccgatcgc attctaaaga aggttgctga agaggcgggg
    17101 gaagtgatta ttgccgcaaa gaatcaaaag cgggaagaga tcatctacga gacagccgat
    17161 ctgctctttc acaccttgct ggtgctgggg taccacgatg tgacgctgaa cgaggtgtac
    17221 cgtgaactcg ggacccgctt cggaaagtcc gggatccggc cttcgcctga ggggggatcg
    17281 cgtggataac tgtatttttt gtcgcattgt tgaaggcggt attccggcca aaattgtgta
    17341 ccaggatgat caggttctgg cattcgatga catcaatgcc caggctccgg tccatatcct
    17401 ggtgattccg aaacgccatg tggccgctgt gcaggactgt cgagagggag accaggctct
    17461 cctagggcat ctcctgttaa cctgctcaaa aattgcaggc atgaaaaacc tggctgaatc
    17521 cggctatcgg attgtgacaa atacgggcgc ggagagcggg cagaccgtgt ttcatctcca
    17581 tcttcatgtg ctcggcggga ggcacatggc ctggcctccg ggataatccg ctcctctcct
    17641 cgattgacac ttatgcagac cgtttgttag ggtgaacggt caactttcaa tcgcgcttaa
    17701 tgcactccag ccggctgcag cctggttgga tccctgcggg cacaagcgag gaccgccgtg
    17761 ggccaaggcc tgatttcgga cgacgtcatc aaccaaatca gagaccgagt ggacatcgcg
    17821 gagatcgtgg gtcaacacgt ggcattgacc cgagccggcc agaatcttaa ggggctctgt
    17881 ccgttccatc aagaaaagtc tccctccttt acggtcagct cttcgcgaca gatttttcac
    17941 tgcttcggct gcggcgccgg cggcaatgtg tatacgtttt tgatgaaatt gaccggggcc
    18001 ggctttcctg agattgttcg tgatctgggc cgcaaggtag gggtggatgt tccggagtcg
    18061 gccggtggat attccagcga ggcgcgcacg cagctgggtc ggctggagcg gctcaatgct
    18121 gcggccgggg cctattatca gcgcatgtta ctggataaca ccgccggtgc tcctgcccgt
    18181 gcttatctcg acgggcgcgg cattcagcac aacacaatgg cgcagtttca aatcggctat
    18241 gccctgccgg agtgggatgg attgaccaaa gctatgttga aagagggctt cgcccctgcg
    18301 gatctggttg cggcgggcct gtccattccg cgagatcaga gcggacaaca gaaagccgcc
    18361 gtgtcaggcc actacgatcg attccgcgcc cgggtcatgt ttccgatcat ggatctgcgc
    18421 aagcgggtcg tggcgttcgg cggacgtatt ctcggcgagg ggatgcccaa atatttgaac
    18481 tcccccgaga cgccgctctt taagaagggg cagaccttgt tcgccctgga agcagctcgt
    18541 gaggcggcgg ggcagcaaca cacattgatc attgtggaag ggtatttcga tgccgtggcg
    18601 ttgcaccagg ccggcatccg ccatgtggcc gccacattgg gcacagccct gaccgccgaa
    18661 cacatcaccg tgatcaggcg ctttgcctcc aatgttgtgc tgctgttcga tcccgatcag
    18721 gccggggtac gggctgcact gcggacgttg gatttattcg ttaatagcgg gttgagcgtc
    18781 aaggttgtct cgcttccgga gggagatgat cccgatacgt acgtgaggaa atacggcgcc
    18841 gaagggttta cggctttaca tgaagcggcc ccgagtctgc tggattttgc cgtggagcat
    18901 agtctgcgga cggctgagtc cgggaccgtg gaagaccgga tccgcgctgt ggatgaggtg
    18961 ttacggattc tgcagaaaag cgcccatccc atcgagcgag aggaacggat tcgtttagtt
    19021 gcggaacggt tgggactcag ccagcaacga ttgattgatc gctatccgac gctggcatcc
    19081 gaggagacac gccggccttc ttcccggccc acggccctgc ccgctgcggc ggcgtcgaaa
    19141 ccgaagggca atccggagga gcgtgatctg gcacatctct tggtgcaggg aagtctgtcg
    19201 gccgaggatc ttcggaagtt gtcgccggac gccttttccg caccggccta tcgccgactg
    19261 attgaatgcg cgatgcagca tcttgagcag gatgggcggg tgtccgtccg gggcctgctg
    19321 gatgccttga tcaatgacga gaactgtggc tcattagtgt ctgagttgtc gatgctggag
    19381 cagcattatg atgatgtccc ggcacatatt gccggatgtt tggaaacgct ggagcggaga
    19441 ggccgtgagc ggaccatggg ggccctgatt caggagttga aggccgccga acgggagcgg
    19501 cgtgaagcgg atgtgcaccg cctgaacgga ttgattaacg agatgcggat tcggaaggcc
    19561 ggcgtgatgc cggctgcgct gacgtctgcg gtgaaggagt aggtatgccg aaacaagaat
    19621 tactcggcga ggtgaagaag ctcatcaatc tcggcaaaga gaagggcttt ctcacctacg
    19681 acgatctcaa cagcacgttg ccggccgatg tcgtgtcatc ggaccagttc agcacgatca
    19741 tgacgatgtt cggggaaatg gacattgaga ttgtggagtc tgctgagacc gaacgcgccc
    19801 cgaaggccgc tgaaggcgaa gaggccatcg aggaaagcga agaagccgag gcgtccgagg
    19861 aaaatgaaaa agagatcgat ctgacacctg gggccctcag ccgaaccgac gatcccgtca
    19921 ggctctacct gaaagagatg ggaagcgttg cgctgctgag ccgcgagggc gaaatcgaga
    19981 ttgccaagcg gatcgaagag ggcaagaaag atatcgccac ggtcgtctac ggcctcccga
    20041 tgactctgga gttcgtgctc aatctgcggg atcagctgaa gaacggcaag atcgatgtgc
    20101 gggagatcgt gccggtggtc gagacggaag aagactttga agaagaagcc gtcgagaagg
    20161 actacgaaga gctgcgggtc aaaacgctgg attctctcaa tgcggtccgt aaggtctcgg
    20221 cctcgctgaa agacttgtac gacaaggccc gccatgtggg cgccgatcct gagaagcaga
    20281 agaaactgcg caaacagatc gatacggtgc gcgggcaggt cgtcgacaag atggagtcgg
    20341 tcaatctgca cggtgtgttg aaagaccgga tgacgcagcg ggttcgggac ctcaatctgc
    20401 tgtttcgcca agcagaacgt gaagtcacga gctgtcaacg ccggttgggc gtcggcggtg
    20461 aagccggtgc ggagctcttg cggcggttgt gccggacaca caaggatctt ctggcggtca
    20521 aacggcggac cagcctctct gaagaggtgc tgcaggacat caagaagcac tatcaggcag
    20581 ccaagggccg gattcgccaa cttgagacgg aagaggcgct gatttctgcc gaagaaatta
    20641 aggacgcggt caagcatctc gatgtggccg aagaaaaagt gaagcgagga aaggcggagc
    20701 ttgtcgaagc caacctgcgg ttggtcgtga gtatcgccaa gaagtacacg aaccggggtc
    20761 tgcagtttct ggatctgatc caagagggga acatcggcct catgaaggcg gtcgacaagt
    20821 ttgagtacaa gcgcgggtac aaattcagca cctacgccac ctggtggatc cggcaggcga
    20881 tcactcgtgc cattgccgac caagcccgga cgatccgcat tccggtccat atgatcgaaa
    20941 cgatcaacaa gctgatccgg acctcacgcc atctggtgca gaagctcggt cgcgaaccga
    21001 ccccggaaga gatcgccgag cgcatggact tgccgctcga caaggtccga aaaatcctga
    21061 aaattgcgcg cgaaccgatt tccctcgaaa ccccgatcgg cgaggaagaa gacagtcact
    21121 tgggcgactt tatcgaggac aagaaggcgg tctcgccgtt ggaagcggcc attcggtatg
    21181 acttgcaacg ccagatcaac ggcgcgctgg aaaccctcac gcctcgagag gaaaaggtct
    21241 tgcgcaagcg gttcgggatc ggcgaggcca ccgatcatac tcttgaggaa gtggggcagg
    21301 actttgaagt gacacgtgag cgcatccggc agatcgaagc gaaagccttg cgcaagctcc
    21361 gacatccgag tcgcagcaag aagttgcgca gttttgtgga gagcctctag gcgcccagcc
    21421 acgggtggtt tgactctgct taagcggcag cctagaatcg agcccgctcg aaccaggtgt
    21481 gggtggcgtg tgcatctccc agggccggtt ggcataggcc atcgcatcag gcggactatg
    21541 ttcagtgtac tggctcgcag ggctgggccc atagctcagg tggttagagc ggctgactca
    21601 taatcagctg gtcctaggtt caagtcctag tgggcccacc aacctgagtg cctcacgatg
    21661 aattccgtgc gaacggtgtc cggtcgacat cggtatcgca taacccacaa ggagcccgtt
    21721 gaacctacag ctttcccccc tcattgaatt gcaaaaactc gaccttcgga tcgccgatct
    21781 caaggagcag cggcgaaaaa tccccgagcg gctggaagaa aatgaggcgc cgcttcgcga
    21841 ggcgaagcgg ttgtttcggg aggcgtccgc ttccgtcgaa tcgctcgtta aggatcgccg
    21901 gggtcacgag aaggatcttg aggcgcacga agaccgtatc ggtaagatga aggaccgcgc
    21961 cgcccagtta aaaacgaatc aggaatatca ggcgcatctc ttcgaagtgg aacttgccaa
    22021 taagaagagg ggcgagattg aagaaaaaat tctgctggcg atggagcaga tcgagcagac
    22081 gcagcgaacc atcgtcgatg tgcaggcaaa gctgaaggaa tccgaagccc tcttcatcaa
    22141 agagaaggca aagctggacg agaaggaccg gctgcttgca accgagctgg ccgaactaga
    22201 ggtcaagcaa aagcacctgt cgggacaagt cgaaaaatca ctcctggccc gatacaacaa
    22261 actgaaaact acaaggaaag agcaggcgct ggcactcatc aaggacggca tttgcgtagg
    22321 gtgccgtctt caattgccgc cgcagctggt ctcgcaggtc aaacgcgggg aagatgtcca
    22381 tacctgcccc tattgctatc ggatgctgta ttgggaaggg gacccggcta ccgaggcgaa
    22441 ggggactgtg gatcacgatc aagcgaagaa cttggaagtc ggtgaatcct tctaagtttc
    22501 tcccaggacc tgcttcgttg ttttgaagtg cagcttggcg acggtcccta gccggtcacg
    22561 tccgtatttc ttgaccacca tccgcccggc cagttccacg gcgggtgatg cgcccttggg
    22621 tagcgtcgtg ttcagctctt gagagagtcg atcgaggcgc tgaagaaact ccgcccgagc
    22681 caggattgag gccgctgcca cagccaggtc ggattccgct ttcgtacgtt gaaccagccg
    22741 gatctgtttt ccctttactt gcagtgcatt caagatcaat cgctcgtccc cgaactggtc
    22801 tgcgatcgcg aggtcgcact cgacttgctg gagcagattt tccagggcac gggcatgtcc
    22861 ccaagccagg aggcgattca aattcttgat cttggcatac agctcgttgt atcgctgcgg
    22921 cccgatggcc acgagactat gggggcagag caggcggatg tcgggcgcca gttcgaggat
    22981 ccgcccatcg gagattttct tgctgtcccg gacctgcatc agggctaagt cctgctccaa
    23041 ggccggtgtg acgaacacag cggcgatcac taacggaccg aaatagtccc ccttgccgga
    23101 ctcgtcgatt ccgatgcgat cgagcgatga gaggctggtg gcggcaggca ctcggtgaaa
    23161 ctcccatgta gcaactggcc gggcggcgcg gtaatctagc agagctcacc ggactggtca
    23221 atttgcggag gcgtgtgatg gaggctcaac agaagatggt cagaagacgg acgtccatga
    23281 tgcggtcgaa catgctgtgc ctcgcgggtg ttgtcgtggc catgctggtg accacaggct
    23341 gccaaaccaa cccctacacc ggacgctggc agctcatgat gatgccgatg tctcaggaaa
    23401 cgcagatggg cgcccaggct tatgccgaag tgaagaatga tccgaagatg aaaacgtcga
    23461 ccgacccgcg ggagattgaa ccggtgaaac gggtggcggc gcgggtgatc gaagcagcca
    23521 agcgttctaa gtacagcgag atggcgaatc aattcgagtg ggaagtgacg gtcatcaagg
    23581 acgacaagac gatgaacgcg tttgcgctgc cgggcggaaa gatcgccgtc tacaccggta
    23641 tttttccggt ggccaagacc gaggccggac ttgcggccgt gatgggccat gaggttgtgc
    23701 acgcgttggc acggcacggc ggggaacgga tgagtcagaa taccttggcg cagacgacct
    23761 tgcaggcgat cggaattgcg ctcggtgtga gtggcgccaa tccggtggtt tcgcaaggag
    23821 cgatggcagc attgggtgtc ggtgcacaag tcggggtgtt gctcccgttc agccggaaac
    23881 atgagtccga ggccgactat gtcggggtgt tgctcgcagc ggatgccggg tacgatccac
    23941 gggaatcgat tcagctttgg acgcgaatgg gcgaactgtc cggcggcaaa tcggcgtcgg
    24001 aattcatgtc gacccaccct agccacgaga ctagaatcga gcagctggag gaatggatgt
    24061 cagaggccat gccgatctat cagtcgaagc caccggcgcc caaccacgag cttccagcgt
    24121 tgcgttagtg ccggagggct cgccgcttga atccctatcg ggcggctcgt atactttcgt
    24181 tgcgcgttgg gggggcacgg gtgctcgctc gtcgagcttg ctcgacggga ggaaagtccg
    24241 gactccaagg gcaaggacgc tggataacgt ccaggcggag
//
LOCUS       DAOQRM010000021        23456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1411_length_23456_cov_17.123029, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000021 DAOQRM010000000
VERSION     DAOQRM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23456
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1411_length_23456_cov_17.123029"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..2527)
                     /locus_tag="PKK81_03465"
     CDS             complement(323..2527)
                     /locus_tag="PKK81_03465"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNV31957.1"
                     /translation="MTLDRAFRLSSILLAATGFASLTLAIALPFWLLALAGAAFAVAL
                     LRVNHAPGFSELFPHLRLSALTWNIFLLLAFAGFWIDLLLVSQELLPAGIHFLVLLLV
                     NKLSNLDQRRDFLHLYAISLITLLASAALTTQIWYAPFFFVYLVAGVWTLLLYHLLQE
                     HEESTGHHLLGSQAAPLDSTRPHITARFFWTTNAMAAGAFGLTLFFFFSIPRVGVGLL
                     QHSHGESLRTTGFSEQVDLGVMGPVKQDPSIVMRVELPDSPGTPAARGPLYLRGVAYN
                     RYDGKSWSNNLLHRRMLTELPQGTFTLRTPGAKPPPQARALRQDILLEPLDTAVLFGA
                     PLAFAVKGDFHSVQSDLMGSLHLAFPSHSRIQYTVYSVPTSLNPAERAAAAVLYPEFI
                     LQQYLQIPPVSPQILDLAQHVTQSATSVSQAVTLIHTHLLANYRYNLDVPSLQSAHPL
                     EDFLLTRKTGYCEHYATAMVVLLRATGIPARLVTGFLATEWNEFGGYYTVRQRDAHAW
                     VEVYFPRSGWITMDPTPPAPENIGQTWWQSADRVMDSVRLQWDRLFVHFSANDQLTVV
                     QGIREGGEAVRTKLSESLNSLSAQSSAMFRRFMSSFTSTGIPETVLLIILAIGATYAG
                     RRLLRSSRNTTAPPDVLSAHQRTVVTLYTAMLHCLAQRGIVKSASATPMEVLRHVRDE
                     WAEAWPSADALTRLYTRVRFGHAPLTPEDHTAAENLLRRLHTLERATTRSQQ"
     gene            complement(2499..3641)
                     /locus_tag="PKK81_03470"
     CDS             complement(2499..3641)
                     /locus_tag="PKK81_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNV31958.1"
                     /translation="MATNKLQALFRRLFRDRTIRVTTEGIRFLLLTLAVGIAAVNTGN
                     NLFYLLLAMMLSLVVLSGLLSEQCVRRLDVHRHLPDYLFVNQPTTATLRVANYKSRIP
                     SVSLRLLDVVAGKDLDRGIHITHLAPAASTLCSYPLLVRQRGPYRLDGIRIVTPFPFG
                     LFYKKSFYPLEATLIVCPEIIPLPPLHLQELNTLGQDHARARRGPGNSLYNLREFRPG
                     DDSRAIHWMTTARTSKLMLKETEAEERRSITLAISTVAPDEAENSFERALSIGASLID
                     HFLKDGYQVRLLLGDQQDILACGTDQALHLFHALALCERGPMATGAAIRHSMARALAE
                     LNEGPTILLSPWTDPARNEQFPSVDYIVSPQSHRDLFDDTGSSLSA"
     gene            complement(3719..4660)
                     /locus_tag="PKK81_03475"
     CDS             complement(3719..4660)
                     /locus_tag="PKK81_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV31959.1"
                     /translation="MNSSQSIKSLQDNIAQVIKGKPQVIEMAVVCLLARGHLLIEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGISMFNRQKQAFEFMPGPLFANIV
                     LADEINRTTPKTQSSLLEAMSESQISVDNQTYPLQQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLRIGYPTPDEEKKVLDRPQLLHPADEIQPVLSAQHIVDLQAQAEKVRMED
                     SLMDYLLAIVLATRHNELLSLGVSTRGALALCRAAKALALVRDRAYCLPEDIKELAPT
                     VLSHRVMLSRSQGMRTNTVEHTEQVIQDILETVPVPV"
     gene            complement(4819..6147)
                     /locus_tag="PKK81_03480"
     CDS             complement(4819..6147)
                     /locus_tag="PKK81_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31960.1"
                     /translation="MHVDSDNLAAFRKQITSARSLDAQAWEASRHLVNAAHWPMTLQA
                     LVNAIETSTVPDTIKRSLVEALPPEHESTNRIASTESLKHLTGLPPSKALRALCIFFG
                     VRSKPSSKWPLPAVTADTIDVLIRRHHNPFDLLTQENPASVLDLGAGDLSFAEELATL
                     YEPQLAAQSRPLIFHCLDRLHPGSQLGGPLHAHPLRLHRLRSRPGLQFRFYGDQDMFA
                     LAPLEQDHRLAERYLIVTCWAPATPTFAYEPTRLSPACLAEELRRTKGESRQVRHGKE
                     SALEVQHAGRSLLFPPWKFDIRGPLALLELMATRGALCVLGAVDSQVFWEILSQLIED
                     PRVRPRDLILSAQNVPEVFGDTYHKLSALPIGGSCLLSDLTPLRRTFPSVLHRPADRT
                     AAYRFRQVTIQRGALFEGRPASSTARRFQGMAEETPPWFLTLVPEPVQTA"
     gene            6287..7303
                     /locus_tag="PKK81_03485"
     CDS             6287..7303
                     /locus_tag="PKK81_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNV31961.1"
                     /translation="MNTFTPSLFRKTRPVSGRLLALLGLVLMMFGCQAKDRSAMVTHR
                     VWDEWAVGQVVDARTGLAVPMQPWLEGLATYDVIYLGEEHHNRSHIDAALTVLRSLMS
                     QGRRPWLAMEMFGWDGQPALARYLRSEESIRSEFLEQVAWKQNWGGAFEDYEPLAQFA
                     KDQRLPLLAMNPPKSLIRQVVKQGLVQAKEQPEWRQWGMEGEVIVDAPTYRSRILSQL
                     QDCHGGGSPEDYQTMYEASMVRDEGMAKTLAAAMKQLRVEASSGQGPLLSYTGGGHVQ
                     YRLPVPDRVTRRVPEGLKQVTVYMATFERERAAELHQAMQEGIADYVWLTPQGAQGPP
                     RRCR"
     gene            7572..8384
                     /locus_tag="PKK81_03490"
     CDS             7572..8384
                     /locus_tag="PKK81_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31962.1"
                     /translation="MISSVVEPNAFQIGKQYQAERRVQMTDASDTEVTSSVMGNSGLY
                     EQTIRLIDGHLEAKCSCTLSEQPICRHGVAALLEYQRWSKARVVPKPRVAAPRPEAEK
                     PAAPVVSSGDMKLSELNQFTEWMQRTVQAIRAGKPLPDQPSIGPGLVLTWTQVIQQMD
                     ERRRESEVAQVELDADIRNREAVVARLTQDLEASVNESKSLQVICRDLQREIEAQKAA
                     VSKTTDLARQVEQVEVEVKAMAALLTDKGRRLEGLSDTCRDVSSMLKSLGKA"
     gene            8525..8701
                     /locus_tag="PKK81_03495"
     CDS             8525..8701
                     /locus_tag="PKK81_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31963.1"
                     /translation="MNKLIAGLIAGIGALQATSAFANVSEGPPDYSGITGLYYTLIAL
                     ILAFGVYDTFFKKS"
     gene            complement(8763..9509)
                     /locus_tag="PKK81_03500"
     CDS             complement(8763..9509)
                     /locus_tag="PKK81_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNV31964.1"
                     /translation="MALVLSGLSGCDQSNTSTVPSSPDSLASATPPSSEQTPPRESLP
                     ATPSPSPDDRPRIIAFGDSLTAGLGVSPEQSYPTQLQKQLDTLGYHYQVLNAGVSGDT
                     SAGGLRRVSWVLAGKPRVVILELGGNDGLRGLGLPETRSHLDAIIRQFKDAHVRVILA
                     GMKLPPNYGEEYTARFEAIYRDLAQLHGLPLIPFLLEGVGGEKALNQSDGIHPTGEGY
                     RIVVENVLRSLLPVLKDASTNNSSAKKKQA"
     gene            9625..10329
                     /locus_tag="PKK81_03505"
     CDS             9625..10329
                     /locus_tag="PKK81_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV31965.1"
                     /translation="MIATQHVTMQLAAGGQTVTILDDVTIDIPEKQTVAIIGPSGSGK
                     STLLGLIAGLDRPTSGTIWLNGVEITALGEQAMARLRLANVGYIFQSFHLIPTLTALE
                     NVSIPLELAGDSQAPRRAEELLHAVGLGHRMSHYPVQLSGGEQQRVAVARAFACRPPI
                     LLADEPTGNLDSSTGQQVIELIMALHRDAGTTLVLVTHDQHLAASMERVITLRDGRVA
                     SDQLASLHHRMPDLES"
     gene            10326..>12094
                     /locus_tag="PKK81_03510"
     CDS             10326..>12094
                     /locus_tag="PKK81_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV31966.1"
                     /translation="MIPFWLMMAWRELRSAWRHFLYFLGCIALGVGAVVGVSLFSTNV
                     ERAVLKEARGLLGGDLEIRLSRPVGAAGSVVLQSLAERGIMRTRVSELVAMVARVDRA
                     HGAADVTQLVELKAIESGYPLYGIVRVEPDRPLMELLRQTGHSCREACHGAVVQESLL
                     IRLGLVVGDAIKIGQASFRITGVIHTEPDRMANMFSLGPRVLISQDGLLAADLVKPGS
                     RLRERHLLKLPASMSLSPLLHELQGRLSAESARLSSYRDAQPQLKQFLDQLARYLGLV
                     GLTALFVGGIGVALSIQAFVREKLQSIAILKTLGADTKTIIYSYLGQAVGLGVLGSAV
                     GIGIGLLLQAVLPQAVSTLLATDVLQQVEFTSVLSWTALAPLSKGLGLGVLTTLLFSL
                     WPLLTIRTIKPAVIFRREVEGAIRPGVRPETSVWGRAAQVVTADPVRAVTAAGIGLGL
                     AGLSVWQAGSLMVGGLFIGGLLVAVVALTVTAKALLLGIRVLPSPRALSLRQALGNIQ
                     RPGGQTLGVMVSIGVGVMVILAIALLEQALVRQVGENRPNDSPTFFFVDIQPDQAEGF
                     SALIHRRTGDVAPDLTPLVRSRL"
     assembly_gap    12095..12194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12195..12800
                     /locus_tag="PKK81_03515"
     CDS             <12195..12800
                     /locus_tag="PKK81_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNV31967.1"
                     /translation="SIRKVEWGNFSTNFYMIFSPGALDGAPMTYVATVRVSPQDEVAL
                     QSAVVAAFPNVTAINIGEVLSSFARVLDRLSLAIRAVALFCLLAGALVMAAALAATRY
                     RRLYEAVILKALGATRALIARSFAAEYAVLGCVAGVIGVGLASLFSWAILRYILELPW
                     SLEPSLLGIGLGCTVLLTLIVGFLSTYRLLGQPPLTVLRHE"
     gene            complement(12831..15422)
                     /locus_tag="PKK81_03520"
     CDS             complement(12831..15422)
                     /locus_tag="PKK81_03520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNV31968.1"
                     /translation="MGSSMTTHDPYRLPRHVLPSHYDLRIEPDLQAHSFTGHEVVTLT
                     VTEPTTEILLNATELEVSTATLSGEGTPPRTGTVRMDEEHQRCYITFPSVIQPGAWKL
                     SLAFRGTLNDKLRGFYRSSYKDEQGHSHSLAATQFEATDARRAFPCWDEPQFKAVFAV
                     TLAIDPALTAISNTRIVDDRQEGGKRVLRFAESMKMSTYLVAFIVGKLDATTPTMARQ
                     TPVRLWSVPGKQHLTPFGQEIAVYSLNFLADYYGIPYPGDKLDLIAIPDFASGAMENL
                     GAITFRETALLLDQRTATHAEQGRIADVVAHENAHMWFGDLVTMAWWNGLWLNEAFAT
                     FMEMLVVDAWKPEWERWTAFGVSRAAALSVDGLLSTRPIEFPVRAPKEAEAMFDVLTY
                     EKGASVLRMLEQHIGPTVFRDGVRHYLTTHAYGNAETTDLWVSLAHASQQNVPALMNE
                     WIFSPGYPLLSLAIDSSSTLTLTQRRFTYAEGSTAASSGAPAQLWQVPIQLRIHTARG
                     AETRRVMLSDQENRIPLPKDWTSVLANEGGHGFYRVRYSTALLNGLQQTGLQTLAPVE
                     RFNLLNDTWASTIAGMVSPADYLSLTEHFRGEQDPHVWAVMLGSFSTMNHLLSEEDRP
                     LLAAFVRNRLTPTFQDLGWTPRTDERDLVKELRGDVIRALGTLGRDPSVQTQALEAYT
                     DLQQQTRPIDPNVIPALVSILAFTGDAARYEEFLNRFHKASTPQEERRYLFSLAAFRI
                     PELLERTLAKTLTDEIRTQDAPFLVSSLLHNVYIREKAWEFVKTNWERMDRQFPKSGL
                     RRMCGGITGLSTPELEQDVRAFFTSRKIDLGGKTLEQYLEQLHIAVRFRERDRDAIRA
                     VLARVAM"
     gene            complement(15605..16306)
                     /gene="rpiA"
                     /locus_tag="PKK81_03525"
     CDS             complement(15605..16306)
                     /gene="rpiA"
                     /locus_tag="PKK81_03525"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNV31969.1"
                     /translation="MTLQFDLDSEKKQAALKATEYVQDGMVVGLGTGTTSKHLIIALG
                     ERVRSGLKIQAVPTSHDTAALARQSGIPLIESDNAWMIDVAIDGADQVDPALNLVKGG
                     GGALLKEKIVAAAAKRFIVMVDHTKLVPALGGSFPLPIEVVPFGWGSTARNIEQASGG
                     RAVLRERNGVVFQTEAGHVILDLHMPKIEDPATLEIELNQIPGIVETGLFIGRTSILI
                     VGHAQGADVTLAAES"
     gene            complement(16318..17403)
                     /gene="glk"
                     /locus_tag="PKK81_03530"
     CDS             complement(16318..17403)
                     /gene="glk"
                     /locus_tag="PKK81_03530"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNV31970.1"
                     /translation="MILAGDIGGTKTNLALYDWTTERVEPVREDSFHSADYKTLEEII
                     EEFLSAPLPKPQAEDELGDEPIEASAEGTTEAPKLPPEPIKLTAACFGVAGPVIDNRC
                     RTTNLPWFIDGATLAERFAIPQVRLLNDLEATAHGLLLLNPDEIVVLNAGAPPKKKQA
                     LALIAAGTGLGECILYWDGSRYRPMPSEGGHTDFAPNSDSEIDLLRHLRGSYLHVSYE
                     RIVSGPGLHAIYEYLRDTKKNEPTWLAEKIKAGNPAAEIAEAGLKGQAEIAKQALDLF
                     ASIYGAEAGNLALKALTLDGVYVAGGIAPKLLKKLQDGSFMRGFTNKGRYKRIMSQIP
                     VKVVMNDKTALLGAASMAAQLTSSTTS"
     gene            complement(17421..18182)
                     /gene="pgl"
                     /locus_tag="PKK81_03535"
     CDS             complement(17421..18182)
                     /gene="pgl"
                     /locus_tag="PKK81_03535"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNV31971.1"
                     /translation="MSQAPAIHTFTDAQELTRAAAGLFLEVGKQAIAERNRFLVALSG
                     GSTPKALYSILANDKYAQQLDWSKVHFLFGDERSVPPTHADSNFAMANAILFSPLHIP
                     SAQIHRMRGEDPPETAAAQYETTLRHLTTAVPGQWPRLDLVLLGMGDDGHTASLFPGT
                     ASLTEQTRWVVPSTSPQGTRARVTLTLGVINHASVVLFLVAGRNKAAVVRRVLEQRPG
                     DPGPYPAALIRPETGRLLWYLDRAAASELTATTDD"
     gene            complement(18184..19887)
                     /locus_tag="PKK81_03540"
     CDS             complement(18184..19887)
                     /locus_tag="PKK81_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNV31972.1"
                     /translation="MPTRPTASLLLDNPTISKTLDDLASSHTIERIWTRDHTLWKPSP
                     TEIDNRLGWLTVLDHMQDGLAELRSFEQAAREARITDVVLLGMGGSSLGPEVLRCTFG
                     SAKGSPKLWVLDSTVPGWVRQVTKAIQPARTLFLLASKSGGTIEVMSLFAHFWELVRR
                     TKGNRGGAQFVAITDPGTGLEELARERGFWRTFTNQPDIGGRYSVLSYFGLVPAALLG
                     LDVSKLLSRALAMREACRHTTHPKDNPGAALGGMMAAMAMAGRDKVTLLTSPALNSFG
                     LWVEQLLAESTGKEGTGIVPVAGEPLASPTAYGADRLFVTLRLKGDRNAALDRSIITL
                     QRAGHPVFRLNLADRYDLGGEFFRWEFATAVAGHALGIHPFDQPNVQESKDNTGRVLK
                     EVETQGRFPSLPMLTPKQALTQLLEQATPNRYLAILAYTTPSPQLDASIRALRKAVML
                     RHHIATTAGYGPRYLHSTGQLHKGGPNSGLFLELVDSMKPDLPVPGKFYSFGTLAQAQ
                     AVGDLQSLQTHQRPAVRIALGPNPVRTIRSLIAMLTPARAGRRKPAAKKRPLPKRRTH
                     R"
     gene            complement(20063..21211)
                     /locus_tag="PKK81_03545"
     CDS             complement(20063..21211)
                     /locus_tag="PKK81_03545"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNV31973.1"
                     /translation="MRRLTHIFRTFCSLALAGLLAMSLGGCVETVPDELVEAIEGIDR
                     DLVTLRASDISPEAYSKFSREWISLRARIQSEENIVRWPWEENELETDLESLQVEGSQ
                     LVADVNTRLQAQRTAAETKLAKVEQRLRLLNTRVGDIGSRVVLGEHPVETELLVRQAR
                     LFIEQGQFDHAIQASDRAGKILLTQTALLTNELGRYADDDLIAAWRESAQRTIEWSRT
                     HRAQAIVISKADRVLSLYKNGKKVLTYPIRLGSRGIRAKQHYGDGATPEGEYRIHRKR
                     GSGQTPYFRALILDYPNADDRRRFEEAQQSGLIPKSQHMPGLIQLHGIAQGITDQPYG
                     SILLDNPQIAVLFDQVSVGTPVTIVGALESQNSISLVLADLGDQEEET"
     gene            21709..21846
                     /gene="rpmH"
                     /locus_tag="PKK81_03550"
     CDS             21709..21846
                     /gene="rpmH"
                     /locus_tag="PKK81_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNV31974.1"
                     /translation="MSFTFKQPSNLKRKREHGFRKRMSTKNGRKVIARRRAKGRARLT
                     V"
     gene            22338..22601
                     /gene="yidD"
                     /locus_tag="PKK81_03555"
     CDS             22338..22601
                     /gene="yidD"
                     /locus_tag="PKK81_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNV31975.1"
                     /translation="MARHITKAAVGPFWGTLALRRLCIVLLMIYRACLSPLLGPACRF
                     HPTCSVYAQDAIERYGVLKGLLMAGRRLLKCHPFHPGGVDPVR"
     gene            22648..>23456
                     /gene="yidC"
                     /locus_tag="PKK81_03560"
     CDS             22648..>23456
                     /gene="yidC"
                     /locus_tag="PKK81_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNV31976.1"
                     /translation="MEKRVIVFLIVSLAVIIGYDYLLKGMGLLPPSEPVQVATNPSPA
                     AETGKPAGGAGDPAKAASADPVSSVNGAPASPGNATGAASEEQTVEVDTELFRAKFSN
                     RGAVLKSWELKRYSAAVEQSSTPVQLVYSGGRFKGPLSLVTGDQAVTSDLGSALYQVT
                     QDLSHLDSAHPVGHLTFRYHDTQKNVDVEKELTFHHGSYVVDIAIRTQGLTTPVDVTL
                     GTNFGIVEWGEGFIGLMGSASLVDDKVLKETPDGEAERKGDVKWSAIQDKY"
BASE COUNT         4483 a         6254 c         7521 g         5098 t
ORIGIN      
        1 aaaatcgggg aaaccacggc tcaatcaagc ccttacgaaa ttactctttt cagaattgcc
       61 aaggcgaggg tcgcgggttc aaatcccgtt tcccgctcca attcacactt ctcctcagca
      121 cgatctttac ccatatcgtt ttcgacgacc acggttcgtc cctttcggct ggaatcctgg
      181 ccggccttta tccatcattt aggtcgccct gacaggtttc atgttgacgg gcggacttgc
      241 cagccgagca cgccgttttt ccccgctcct gttcatacgc gtttgtctct gtcgagtcgc
      301 tgaaatctgg cctcagtcag gactattgtt gtgaacgagt tgtcgctcgt tccagtgtgt
      361 ggagcctgcg cagcaggttt tcggcagcgg tatggtcttc gggagtgagg ggagcgtgac
      421 caaatcggac tcgagtatag agtcgtgtaa gggcatcagc ggagggccag gcttcagccc
      481 attcgtcacg aacatggcga agcacctcca tgggtgtggc gctcgctgac ttcacgatgc
      541 cgcgttgcgc aaggcagtgc agcatggcgg tgtagagcgt gacgacggtg cgttgatgcg
      601 ctgacagcac atccggcggt gccgttgtgt ttcgggacga ccgcagcagt cgccgacccg
      661 catacgtcgc gccgatggcc agaatgatca agagtacagt ttccggaata ccggtcgatg
      721 tgaacgagga catgaagcgc cggaacatcg cggagctctg ggccgagagg gagttgagag
      781 attcggagag ttttgtccga acggcttccc cgccctctcg aatgccttgc acgacagtga
      841 gttgatcgtt tgcgctgaag tggacaaaga ggcgatccca ttgaagccgg accgaatcca
      901 tcacacgatc ggcggattgc caccaggttt gcccaatgtt ctctggagca ggcggcgttg
      961 gatccatcgt aatccagcct gaccgtggga aatagacctc cacccaggca tgcgcatccc
     1021 gttgtcgaac cgtatagtac ccgccgaact cattccattc agtcgcaagg aatcccgtga
     1081 ccagtcgagc cggaatgccg gtggcgcgga gcagcaccac catcgcggtc gcgtaatgtt
     1141 cgcaatatcc tgtttttcga gtcaggagaa agtcttcaag cgggtgagcc gattggagcg
     1201 agggcacgtc caggttgtag cgatagttcg ccagcaagtg cgtgtggatc agggtaacgg
     1261 cttgggagac gctggtggcc gactgcgtga catgctgtgc gaggtcaagg atctgcgggc
     1321 tgacgggggg aatctgtaga tactgctgaa gaataaactc cgggtacagc accgcggcgg
     1381 cggcccgctc cgctggattg agactggtcg gtacggagta gaccgtgtac tggatgcggc
     1441 tatgagaagg aaacgccaag tgcagcgatc ccatcagatc ggattggacc gagtggaagt
     1501 cgcccttgac ggcgaaggca aggggggcgc cgaacagcac ggccgtatcg agcggttcca
     1561 gcagaatatc ttgtcggagt gcgcgtgcct ggggcggcgg tttggcgccc ggtgttcgga
     1621 gcgtgaaggt gccttgggga agttccgtga gcatacggcg gtgcagcagg ttgttgctcc
     1681 aggattttcc gtcataccga ttgtaggcga cgccacggag atacagcggc ccccgtgcag
     1741 cgggagttcc gggactgtcc ggcaactcca cgcgcatgac aatgctggga tcctgtttca
     1801 ccggtcccat gacacccaga tcgacctgct ctgaaaagcc cgtcgttcgc aggctttccc
     1861 catgggaatg ttgcaacagg ccaaccccta cccgtgggat cgaaaagaaa aagaacaatg
     1921 tgaggccgaa cgcgccggct gccatcgcat tggtagtcca gaagaagcgg gccgtaatgt
     1981 gcggccgtgt cgagtcaagc ggtgcagctt ggctgccgag caaatggtga ccggtgctct
     2041 cttcgtgctc ttgcaacagg tgatacagga gcagcgtcca gactccggcc accagataga
     2101 caaagaagaa gggggcgtac cagatttgcg tggtcagcgc ggcggaggcc aggagtgtga
     2161 tgaggctaat ggcatagagg tgaagaaaat cccgccgctg gtcgaggttg gaaagtttgt
     2221 tcaccaggag cagtacgagg aaatgaatcc cggccgggag aagttcctgc gagacgagta
     2281 acagatcgat ccagaagccc gcgaaggcga ggaggagaaa aatattccat gtcagggcgg
     2341 agaggcggag atgcgggaag agttcggaga agcccggggc gtgattcacg cgcaggagtg
     2401 ccacggcgaa ggctgcacct gccaaggcga gaagccagaa tggcagggca atcgcaagcg
     2461 tcaggctcgc gaagccggtt gccgccagca gaattgagct aagccgaaag gctcgatcca
     2521 gtgtcatcga agagatccct gtgtgactga ggagacacga tgtaatcgac cgatggaaat
     2581 tgctcattcc gggcgggatc ggtccagggt gagagtagaa tggtcggtcc ctcgttcaat
     2641 tctgcaagcg cccgggccat ggaatgtcgg atcgcagctc ccgttgccat cgggcctcgc
     2701 tcgcagagcg ccagggcgtg aaatagatgc agggcttggt cagtcccgca ggccagaatg
     2761 tcctgctgat cgcccagtag caaccggacc tggtatccgt ctttcagaaa gtgatcaatg
     2821 agggaggcgc cgatcgagag cgcccgctcg aagctgttct ctgcctcgtc cggcgcaacg
     2881 gtcgagatgg cgagagtgat cgatcgtctc tcttccgcct cggtttcctt gagcattaac
     2941 ttcgaggtac gggctgtggt catccaatga atggcgcggg aatcgtcccc cggcctgaat
     3001 tcgcgcaagt tgtacaggga gttacccggt ccgcgtcgcg cgcgtgcgtg atcctgcccc
     3061 agggtattca gttcctgcag gtgcagcggc ggaaggggaa tgatttccgg acagacgatc
     3121 agtgtggctt cgagtggata gaaggatttc ttgtaaaaca gaccgaaagg aaacggggtc
     3181 acgatacgga ttccgtcgag ccggtatggt ccacgctgtc gcaccagtag cgggtacgag
     3241 cagagggtgg aggctgcagg cgcgaggtgt gtgatgtgaa tcccccgatc gagatctttc
     3301 ccggcaacga catcgaggag gcgtaacgag acacttggaa tacgtgattt gtaattggcg
     3361 acccggagcg ttgcggtggt cggctgattg acgaagaggt agtccgggag gtgccgatgg
     3421 acgtccagtc gccgcacaca ttgttcagac agcaacccgg acagcacgac caggctcagc
     3481 atcatggcca ataggaggta gaacagatta ttgccggtgt tgacggcggc gatcccgacg
     3541 gccagggtca acagcaggaa acggatgcct tccgttgtca cccggatggt gcggtcccgg
     3601 aacagccggc ggaacagagc ctggagtttg ttggttgcca cgctgatgga ctgctgccgt
     3661 ccagaccgca ttactgcgac atttcgcgtg atgccggtgt tcggccccct gacggttgtt
     3721 acacgggaac cgggactgtc tcaagaatgt cttgaatgac ctgttccgta tgttcgacgg
     3781 tgttcgtgcg catcccctgg gaccgactga gcatgacacg gtgcgagagg acggttggtg
     3841 cgagctcttt gatgtcttcg gggaggcaat aggcgcggtc gcgcacgagg gccagggctt
     3901 tcgccgctct gcacagcgcg agggcgcctc gggtactcac gcctaatgac agcagctcgt
     3961 tgtgcctggt ggcgagcacg atggcgagga gataatccat caggctgtct tccatccgga
     4021 ccttttccgc ctgcgcttga agatcgacga tatgctgcgc gctcagtacc ggttgaattt
     4081 cgtcggccgg gtgcagcagc tgggggcgat ccagcacttt cttttcctca tccggagtcg
     4141 gatagccgat gcgaagtcgc atgaggaatc ggtcgagctg cgattcagga agcgggaagg
     4201 tgccgtgata ctctgccggg ttttgtgtgg cgatgaccat aaacggctgc tgaaggggat
     4261 aggtttggtt gtcgacggag atctgggatt cgctcatcgc ttcgaggagg ctgctttgag
     4321 tcttcggcgt ggtgcggttg atttcgtcgg ccaggacgat gttggcaaac agcgggcccg
     4381 gcatgaactc aaacgcttgt ttctggcgat tgaacatgga aatgccgacg atatcggagg
     4441 gcagcaggtc gctagtgaac tgaatacgtt tgaaggagca atcgagggag cgcgcgaggc
     4501 tatgggccaa cgtggtcttc ccgacaccgg ggacgtcctc gatcaggaga tggccacgtg
     4561 ccagcaggca gacgaccgcc atctcgatca cctgtggttt ccctttaatg acctgcgcga
     4621 tattgtcctg taacgatttg atagattgag aagaattcac gatgggatgt ttctgaagag
     4681 ggttacggtc ggcaccaggg gcgttatgcc agaagtctaa caaagggtct ggagaccgtc
     4741 aatttatacg gactttcacg cgggaggcca ggcggaagct gtagggactg ccacgttggc
     4801 gcgacttttg tgcgcctgtc acgccgtctg gactggttca ggaacgagag taagaaacca
     4861 ggggggagtt tcttccgcca tcccctgaaa ccgccgggcc gtgctgctgg ctggacgacc
     4921 ttcgaacaga gcgccgcgct gaatggtcac ttgtcggaat cggtaggctg cagtccggtc
     4981 ggcaggccgg tgaagtacag aaggaaaggt tcggcgcaac ggtgtgaggt cggataagag
     5041 acaggatccg ccgatcggga gggctgagag cttgtggtag gtatccccga agacctcggg
     5101 tacattctga gcagacagga tgagatcacg gggtctgacc cgcggatctt cgatcaattg
     5161 cgacaggatt tcccaaaata cttgggagtc aacagctccc aagacgcaga gcgctccgcg
     5221 tgtggccatc aattccaaga gggcaagcgg gccgcggata tcgaatttcc agggcggaaa
     5281 aaggagactc cgacccgcgt gttgcacttc aagggcggac tctttgccat gccggacttg
     5341 tcgggactcg ccttttgtgc gtcgcagctc ttcggccaga caggccgggg aaaggcgcgt
     5401 gggttcatag gcaaaggtcg gggtggcggg tgcccagcag gtgacgatca ggtagcgttc
     5461 ggcaagacgg tgatcctgtt cgagcggggc gagtgcaaac atgtcttgat cgccgtaaaa
     5521 gcgaaattgc agtccgggtc tggaacgcag acggtgcaga cgaagcggat gagcgtgcag
     5581 gggaccgccc agttgcgagc cgggatgcag ccggtcaagg caatggaaaa tgaggggtct
     5641 gctttgcgcg gctagctgag gttcgtacag ggtggccagc tcttcggcaa acgaaaggtc
     5701 accggctccc agatccaaga cggaagccgg gttctcttgt gtcagcaggt cgaaggggtt
     5761 gtggtgccgg cggatcaata cgtcgatggt gtctgccgtg accgccggta acggccattt
     5821 cgatgatggt ttcgagcgca ctccaaaaaa gatgcaaagc gcccgtaagg ctttggaggg
     5881 cgggagtccc gtcaggtgct tgagcgattc cgtggaggcg atgcggttcg tgctctcgtg
     5941 ctccggcggc agggcttcaa ccaggctgcg tttgatcgta tccgggacag tcgaggtttc
     6001 gatcgcgttg accagcgctt gaagggtcat cggccagtgc gcggcattca ccaggtgacg
     6061 cgaggcctcc caggcttgtg catccagtga ccgcgccgac gtgatctgtt tccgaaaggc
     6121 cgccaggttg tcagagtcaa cgtgcatgat tcgatcagcg gtgtacggat tcctcggtgg
     6181 tgcggggatg aaagctgctg cgtgtccggg cagagtatcg ggcctgtcgt aagggagtca
     6241 agggagctgc cttgcggcta ctgggtccgt atgttaggta gacaatatga atacattcac
     6301 tccatcgctt ttccggaaga ctcgcccagt ctcgggccgc ttgctcgccc tgcttggtct
     6361 tgtcttgatg atgttcggct gccaggcgaa agatcgctca gcgatggtca ctcatcgggt
     6421 ctgggacgaa tgggcggtgg gccaggtggt ggatgccagg accggtctcg cagtaccgat
     6481 gcagccgtgg ttggaggggc tggccacgta cgatgtgatc tacctgggag aagaacatca
     6541 caatcgatcg catatcgacg cggcgttgac ggtgcttcgc tcccttatga gccaggggcg
     6601 tcgtccgtgg ctcgcgatgg aaatgttcgg gtgggacggt cagccggcgc tcgctcgcta
     6661 tctgcgttcg gaggagagta ttcgctcgga gttcctggag caagtcgcgt ggaagcagaa
     6721 ttggggcggc gcgtttgaag attacgagcc attggcgcaa ttcgcgaaag atcaacgtct
     6781 tccgcttctg gcaatgaatc cgccgaagag tctgatccgg caggtggtga agcagggttt
     6841 ggtccaagcc aaggaacagc ctgagtggcg tcaatggggc atggagggag aggtcatcgt
     6901 ggatgcgcct acctaccggt cccggatttt atctcaactc caggactgcc acggcggtgg
     6961 gtctccggag gactaccaga ccatgtatga agcctctatg gtcagagacg aggggatggc
     7021 gaagacctta gcagcggcga tgaagcaact acgtgtcgag gccagctccg gtcagggccc
     7081 cctgctcagt tatacgggcg gaggtcatgt gcaataccgg ttgccggtcc cggatcgggt
     7141 gactcgacga gttccggaag ggctcaagca ggtcacggtt tatatggcca cattcgagcg
     7201 agagcgggct gcagagcttc atcaggccat gcaagaaggg attgctgact atgtgtggct
     7261 gaccccccag ggggctcaag gacctcctcg acgctgccga taagaatctc cagtggcggc
     7321 acgaaaattc tgcgcggcct cgatccattt ctcacaattc cccccgcctg cgggatggtc
     7381 gttcaggaga acctgtcata cactcaagtc tcgctggata ggtggacgtc aaaggtggtg
     7441 attccatgac actgcggcga tcactcggta ggctgcaagg acgactcagc ggtactaatc
     7501 attccacagg tgacactatg aagggcagaa tcgcgtgttc ggccgatcgt ctgagtttgt
     7561 tggaggcggc catgattagc tcggtggtgg agcccaacgc gttccaaatc ggaaagcagt
     7621 atcaggcaga acgccgcgtg caaatgacgg atgcgtcgga tacggaagtg acgtcgtcgg
     7681 tcatgggcaa ttccgggttg tacgagcaga cgattcgtct cattgatggt catctggaag
     7741 ccaaatgttc ttgcacgtta tccgagcaac cgatctgccg acacggtgtg gctgcgctgc
     7801 ttgagtatca gcggtggtca aaggcacgag ttgtccccaa gcccagagtc gccgctcccc
     7861 gccccgaagc agaaaaaccg gccgcccctg tcgtgtcctc cggtgatatg aagttgagcg
     7921 aattgaacca gtttacggag tggatgcagc gaacggttca ggccattcga gccgggaagc
     7981 cccttccaga ccaaccttct atcggaccgg gccttgtgtt gacctggacc caggtgatcc
     8041 aacagatgga tgaacggagg cgagagagcg aagtcgctca ggttgagctt gatgcggaca
     8101 ttcgcaatcg agaagcggtc gtggcccgtc tcacccagga tctggaggct tccgttaatg
     8161 aatctaagtc actacaggtc atttgccgag atcttcaacg ggagattgag gcgcagaagg
     8221 ctgcggtgag caagacgacc gatcttgccc ggcaggttga gcaggttgaa gtcgaggtga
     8281 aagcgatggc cgcacttttg acggacaagg ggcgtcgctt ggaaggtctt tcggatacct
     8341 gccgagatgt gtcttcgatg ctcaagtctt tgggaaaggc ctgattggca agtcggtggt
     8401 cacggaagct gccaccccga tttttctccc ctattccctc tttgtgaaac ccccttgcgt
     8461 catccagggt cacttcagta caatgcggcc ctctttgggt attccttgat tacaaggggg
     8521 gatcatgaac aagctgatag caggattgat tgccggtatt ggggcgcttc aggcaaccag
     8581 tgcgtttgcc aatgtcagtg aaggtccacc ggactacagt ggaatcacgg gcctgtatta
     8641 taccttgatc gctctgatct tggctttcgg ggtctacgac accttcttca agaagagcta
     8701 accggctttc aggacttcat ggcgtgaaat gttcacccgc ttgatgtggc ggggaggata
     8761 tttcacgcct gcttcttttt cgctgacgag ttgttggtgg atgcgtcctt caacacagga
     8821 agcaggctcc ggagcacgtt ctcgaccacg atacgatacc cttcccccgt cgggtggatg
     8881 ccgtctgatt gattgagggc cttttcccct cctactccct ccagtagaaa tgggataagt
     8941 ggaagcccat ggagctgcgc gagatcccga tatatggctt cgaatcgagc ggtgtattct
     9001 tcgccgtaat ttggggggag cttcattccc gctaagatca ctcgtacgtg agcatccttg
     9061 aactgtcgga tgatcgcgtc gaggtgtgac cgggtttcag gcaacccgag gccgcgcagt
     9121 ccgtcattcc cgcccaactc aagaatcacc acccgtggct tgccggcaag gacccaggac
     9181 acacggcgga gtcctcctgc tgacgtgtcc ccgctgactc ctgcattgag cacttggtag
     9241 tgatagccta aggtatcgag ttgtttctga agctgggtgg ggtaagactg ttccggggag
     9301 actccaagtc cggcagtcaa gctgtctcca aacgcgatga tacggggccg atcgtcaggt
     9361 gaaggactcg gcgtggcagg aagcgattcg cgggggggtg tttgttcgga ggatggagga
     9421 gttgcagatg caaggctgtc gggtgaggaa gggacagtgg acgtatttga ttgatcgcat
     9481 cctgatagtc cgctgaggac caatgccatg aggcaaaccg ttcccaggcg cgcgatacac
     9541 ttcaaaagct ccgagcaggt cagcatctgt acagtataat atgaaatcgt tttgcgggca
     9601 ctccatcgag gaatcatcca agcaatgatt gctactcagc atgtgacgat gcagttggcc
     9661 gcaggcggtc agacggtgac cattcttgac gatgtcacga tagacattcc ggaaaagcaa
     9721 acggtagcga ttatcgggcc ttccgggagc ggtaagtcca cgctcttagg cctgattgcg
     9781 gggctcgacc gaccgacatc tggaactatt tggttgaacg gggtcgagat cacggcgctc
     9841 ggtgaacagg cgatggcgcg actgcgcttg gccaatgtcg ggtatatctt tcaatcgttt
     9901 catctcattc caacgctcac ggcgctggaa aatgtgtcga taccgcttga actcgcgggc
     9961 gactctcagg ccccgaggcg agcggaggaa ttgctgcacg cggtagggtt aggacatcgg
    10021 atgtcgcact atccggtcca gctatcggga ggagagcaac agcgggtcgc ggtggcgcgt
    10081 gcgtttgcct gtcgcccgcc gattctgttg gccgatgagc cgaccggcaa tctcgactcc
    10141 tccacagggc agcaggtgat cgaattgatc atggccttgc accgcgatgc cgggacgacc
    10201 ctggtcctgg tcactcacga ccagcacctg gccgcttcca tggaacgggt gatcacgctc
    10261 cgggatgggc gtgtggcttc ggatcagctg gcgtcactcc accataggat gcctgatctc
    10321 gaatcatgat tcccttctgg ctcatgatgg cctggcggga actacgttcg gcctggcggc
    10381 actttctgta ctttctcggc tgcattgccc tgggagtcgg ggcagtggtc ggggtctcac
    10441 tcttttctac caatgttgag cgggccgttc tgaaggaagc gcgcgggttg ctgggggggg
    10501 acctggagat tcgcctgtcc agacctgtag gcgcggcagg ctcggttgtg cttcagagtc
    10561 tcgcagagcg cgggattatg cgcacacgag tgagtgaact ggtcgcaatg gtggcacgag
    10621 ttgatcgcgc ccacggcgcg gcagatgtga cgcagctggt cgaattgaaa gcgatcgaat
    10681 cagggtatcc cttgtatgga attgtacggg ttgaacctga ccggccgctg atggagttgc
    10741 tgcgccaaac aggacacagc tgtcgagagg cttgccatgg ggcagtggtt caggaaagtc
    10801 ttctgattcg gttagggttg gtcgtgggcg atgcgatcaa aatcggacag gcgtcattcc
    10861 gaattaccgg cgtcattcat accgagccgg atcgaatggc gaatatgttc agcctgggac
    10921 cgcgagtgct gatttcccaa gacggtctct tggctgccga cctcgtcaaa ccaggaagtc
    10981 gtcttcgcga acggcatctc ctcaagttac ccgcttccat gtccctgtct ccgctgctcc
    11041 atgagctcca ggggcgcctt tccgcggagt ccgcacggct gtcttcctat cgggatgctc
    11101 agccacagct caagcagttt ctcgatcagc tcgcccggta cttggggcta gtgggcttga
    11161 cggcgttgtt cgtcggcggg attggcgtgg ccttgtcgat tcaagcgttc gttcgagaaa
    11221 aactccaatc gattgcgatc ctcaagacat tgggcgcgga tacgaagacc atcatctatt
    11281 cgtatctggg acaagcagtt gggttggggg tgctgggcag cgcagtcggt attgggatcg
    11341 ggctcctgtt gcaggccgtt ttgccgcagg cggtctccac cttgctcgcg acggatgtcc
    11401 tgcagcaggt cgagtttacg tccgtgctgt cttggacagc gctggcgccg ctctcgaagg
    11461 gcttgggctt gggtgtgctg accacccttc tgttcagtct gtggccgctt ctgacgattc
    11521 gaaccatcaa gcctgcggtc atcttcaggc gtgaggtgga aggtgccatc cgccccggtg
    11581 ttcggcctga gacatcggtg tggggccgcg cggcacaggt cgtcacggcg gatcctgttc
    11641 gtgccgtcac ggcggccggc attgggcttg gtttggcggg cctctcggtg tggcaggccg
    11701 ggtctctgat ggtcggaggg ctctttatcg gcggcctcct ggtcgctgtg gtggcgttaa
    11761 ccgtgaccgc gaaagcactc ctcctcggga tacgtgtgtt gcccagcccg cgtgcgcttt
    11821 ctctgcgaca ggcgctcggc aacattcaac gtcccggagg gcaaaccctt ggggtgatgg
    11881 tttccatcgg tgtcggggtg atggtgattc tcgcgattgc gttgctggaa caggccttgg
    11941 tgcgtcaagt cggcgagaac cgaccgaacg attcaccgac cttctttttt gtcgatattc
    12001 agccggatca ggcggagggc ttttccgcct tgattcatcg gcgcaccggc gatgtggctc
    12061 ctgatctgac tccgctggtg cgctctcgat tgcannnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnagtatc agaaaggtcg agtggggaaa tttctccacg aatttctata
    12241 tgattttttc accgggagca ttggatgggg cgccgatgac ctatgtcgcg acggtgcggg
    12301 tgtccccgca ggatgaggtg gcgctgcagt cggccgtggt cgccgcattt cccaatgtga
    12361 cggcgattaa catcggcgag gtcttaagca gcttcgcccg ggttctcgac cggctgtccc
    12421 tggccatccg cgcggtggcg ctgttttgtc tgttggccgg ggcactcgtc atggcggcgg
    12481 ctctggcggc tacgcggtat cgtcgtctct atgaggccgt gattctcaag gctctcggcg
    12541 cgacccgtgc gttgattgcc cggtcgtttg cagcggaata tgcagtgctg gggtgcgtgg
    12601 cgggggtgat cggcgtgggc ctggccagcc tcttctcgtg ggcgattctg cgctacattc
    12661 tggagttgcc ctggtcgctg gagccgtctc tgctgggcat cgggctgggt tgcactgtgt
    12721 tgctcaccct catcgtaggg ttcctcagta cctaccgcct gctcgggcag cctccgttga
    12781 ctgtgcttcg gcacgagtga cggccggctg gatgtactcg gccgttccca ctacatcgcg
    12841 acgcgtgcca gcacggcgcg aatggcgtcg cggtcgcgtt cccggaatcg tacggcgatg
    12901 tgcagctgct ccaggtactg ctccagagtc ttcccgccga ggtcgatctt ccgggatgtg
    12961 aagaacgctc tgacgtcttg ctccaattcc ggagtcgaaa gaccggtgat ccctccgcac
    13021 atgcggcgca ggccgctctt gggaaactgc cggtccattc gctcccaatt agtcttcaca
    13081 aactcccagg ctttttcacg aatgtacaca ttgtgcaaca ggctgctgac gaggaagggc
    13141 gcatcttggg tgcggatctc gtcggtcagg gttttcgcca gggtgcgctc cagcagttcg
    13201 ggtatccgga aggccgcgag tgaaaagaga tagcgacgct cttcctgcgg ggtggaggcc
    13261 ttgtggaagc ggttgagaaa ttcctcgtag cgagccgcgt cgcctgtgaa ggccaggatg
    13321 gaaacgagcg cggggatcac attggggtcg atcggtcgag tctgctgttg cagatcagtg
    13381 tatgcttcca gcgcctgggt ttggacgctg gggtcgcgac ccaatgtgcc taaggcgcga
    13441 atcacgtcac cgcgcagttc tttgacgaga tcgcgctcat cggtacgcgg tgtccatccc
    13501 agatcctgga atgtcggggt gagtcgattg cgcacaaagg cagccaacag cggtcgatcc
    13561 tcctcgctca gcagatgatt catggtggag aacgacccca acatcacggc ccagacatgc
    13621 gggtcctgtt cgccacggaa gtgttccgtc aagctgagat agtcggcggg tgacaccatg
    13681 ccggcgatgg ttgaggccca ggtgtcgttc aggagattga accgttcgac gggggccagg
    13741 gtctggaggc cggtctgttg caggccgttg aggagcgctg tgctgtatcg aacgcggtag
    13801 aatccgtgcc cgccctcgtt cgccagtact gatgtccaat ccttcggcag cgggatacgg
    13861 ttctcctgat cgctgagcat cacacgccgg gtttcagcgc cccgcgcggt gtggatgcgc
    13921 aactgaatcg gaacctgcca gagttgcgcc ggtgccccgg aagatgccgc cgtagagcct
    13981 tcggcgtagg tgaagcgacg ctgtgtgagc gtcagcgtgg aggatgagtc gatggcaagc
    14041 gagagcaagg gatatcccgg cgagaagatc cattcgttca tgagtgccgg aacattctgt
    14101 tgtgacgcat gcgcgagcga gacccacagg tcggtagtct ctgcgttccc ataggcatgc
    14161 gtcgtcagat agtggcggac gccgtctctg aataccgtcg gaccgatatg ctgttccagc
    14221 atgcgcagga cggaggctcc cttttcatag gtcagcacgt cgaacatggc ttcggcttct
    14281 ttgggcgcgc gcacggggaa ctcaatcggc ctcgtgctca gcaggccgtc gacggacaga
    14341 gcggcagcgc gtgagacgcc aaaggctgtc cagcgttccc actccggttt ccaggcatcc
    14401 accaccagca tttccatgaa ggtggcgaag gcctcgttga gccagagccc gttccaccag
    14461 gccatggtca cgaggtcgcc gaaccacatg tgggcattct cgtgcgcgac cacatccgcg
    14521 attcgtccct gctccgcatg tgtggccgtg cgctggtcca gcagcaaggc tgtttcacgg
    14581 aatgtgatcg cgcccagatt ttccatcgcc ccggaagcga agtcggggat ggcgatgagg
    14641 tccagtttgt cgcccggata ggggatgccg tagtagtcgg cgagaaaatt gagcgagtac
    14701 acggcgattt cctgaccgaa cggggtcagg tgttgtttgc cgggcacgga ccagaggcgg
    14761 accggggtct gccgggccat ggtgggagtt gtggcgtcga gtttcccgac gatgaaagcc
    14821 acgagatacg tggacatctt catggattcc gcgaagcgca gcactcgctt cccgccctct
    14881 tgtcgatcgt ccacgatccg ggtattcgag atggcggtca gtgcgggatc gattgccagg
    14941 gtgacggcga agacggcttt gaattgcggt tcatcccaac aggggaaggc ccgccgcgca
    15001 tcggtcgcct caaactgggt cgcggccaga ctgtgcgaat ggccctgttc atccttgtag
    15061 ctgctgcgat agaacccgcg caatttatcg ttcaatgtgc cgcgaaacgc caggctcaat
    15121 ttccaggcgc cgggttgaat gaccgacggg aaggtgatgt agcatcgctg gtgctcctcg
    15181 tccatccgta cggtcccggt gcgcggcggc gtcccttcac cggacaatgt cgctgtcgag
    15241 acctccaatt cggtcgcatt caggagaatc tcagtagtcg gttcggtcac cgtcagcgtc
    15301 acgacctcgt ggccggtgaa cgagtgtgcc tggaggtccg gctcgatacg caagtcgtaa
    15361 tgagacggga ggacatggcg cgggagtctg tagggatcgt gagtcgtcat agatgaaccc
    15421 atagtgtgtg tcttcggccg tcgatggttg tgtggcggat tcgagtggct ccaggccgcg
    15481 ggcacgaaga accaggaggt tgcgccatgc caggctccca accacaccac tgctttggtg
    15541 aggactcgtg tggccagcaa cgccgcatgt cgccgagtga cgggagggtc ggtccggtcg
    15601 cccgtcatga ctccgctgca agggtgacgt cggcaccctg tgcatggccg acgatcagga
    15661 tgctggtgcg tccaatgaac agtccggtct caacgatgcc gggaatctgg ttaagttcta
    15721 tctcgagggt ggccggatct tcgatcttgg gcatgtgcag atcgaggata acgtggcccg
    15781 cttcggtctg aaacacgacg ccgtttcgct cgcgcaagac cgctctgcct cctgaggctt
    15841 gttcgatgtt ccgtgccgtg ctgccccatc cgaacggcac gacttcaatc ggcaatggaa
    15901 aactgccgcc gagcgccggc acgagtttcg tgtggtcgac catcacaata aagcgcttcg
    15961 ctgcggcggc tacaattttt tctttgagca acgcgcctcc gccgcctttc acgaggttca
    16021 aggcgggatc gacctggtcg gctccgtcga tggccacatc gatcatccat gcgttgtcgg
    16081 actcgatgag gggaatgccg gattgcctgg ccagagccgc tgtgtcgtgc gaggtcggca
    16141 cggcttggat tttgagccct gagcggacgc gctcgccgag ggcgatgatc aaatgcttcg
    16201 atgtcgtacc ggtccccaat ccgacgacca tgccgtcttg cacatattca gtggctttca
    16261 gcgccgcctg tttcttttcg gaatcaagat caaattgtag tgtcatgatg gatggcgtca
    16321 tgatgtggtc gatgatgtga gttgtgctgc catcgaggcg gcgccgagca aggcggtttt
    16381 gtcgttcatc acgaccttca ccggaatctg gctcatgatc cgtttgtaac ggcccttatt
    16441 tgtgaacccg cgcataaacg agccgtcctg cagtttcttc agtaatttgg gcgcaatccc
    16501 gccggcgaca tagaccccgt cgagtgtgag cgccttgagc gccaggttgc cggcttccgc
    16561 gccatagatg gacgcaaata aatccagggc ttgtttggcg atttcggcct ggcccttgag
    16621 tccggcctcg gcaatctcgg cggcaggatt accggctttg atcttctccg ccaaccaggt
    16681 cggctcattt tttttggtgt cgcggagata ttcataaatg gcatggaggc ccggcccgga
    16741 tacgatgcgt tcgtaactga catgcaggta gctgccgcgc agatggcgaa gcaagtcgat
    16801 ttcgctgtcg ctgttggggg caaagtccgt gtgcccgcct tccgacggca tggggcggta
    16861 gcgacttcca tcccaataga gaatgcattc cccgagcccg gtaccggctg cgatcaaggc
    16921 gagggcctgt tttttcttcg gcggggcgcc ggcgttcagt actacgattt catcgggatt
    16981 caagaggagg agcccatggg ccgttgcttc aaggtcgttg agcaggcgca cctgggggat
    17041 ggcgaatcgc tccgcgagtg tcgccccatc aatgaaccag ggaagattgg tggtgcggca
    17101 acggttatcg atcaccggcc ctgccacgcc gaagcacgcc gccgttaatt tgatgggctc
    17161 tgggggaagc ttcggtgcct ccgtagttcc ttctgccgac gcttcgatag gctcgtctcc
    17221 cagctcgtct tctgcttgtg gcttgggcaa cggtgcactg agaaattctt cgatgatttc
    17281 ttcgagggtc ttatagtccg cgctatgaaa gctatcctcc cgcaccggct cgacccgttc
    17341 agtcgtccag tcgtagagcg ccagattggt ttttgtgcct ccgatatcgc ctgccaggat
    17401 catggtgcct cttgcgatga ctaatcgtca gtggttgcgg tcaattcgga tgccgcggcg
    17461 cggtcaagat accacaacag tcgtccggtc tcaggccgaa tcaaagctgc cggatacggg
    17521 ccgggatcac cgggccgctg ctccagtacg cgtcgcacaa ccgcggcttt gttccttccg
    17581 gcgacgagga acagtaccac actcgcgtga ttgatcacac ctagggtgag agtcacccgt
    17641 gcccgcgttc cttggggaga cgtgctggga actacccaac gagtctgttc ggtcagtgat
    17701 gccgtgccgg ggaagagtga ggcggtgtgg ccatcgtcgc ccatgcccag aaggacaagg
    17761 tccaaccgcg gccattgccc ggggacggct gtggtgaggt gacgcagggt ggtttcgtat
    17821 tgagctgccg cggtttcagg cgggtcttcg ccgcgcatcc tgtgaatctg cgctgaggga
    17881 atgtgcaacg ggctgaacaa tatggcgttc gccatggcga aattgctgtc ggcatgcgta
    17941 ggcggtacgc tgcgctcgtc tccgaacaga aagtgcacct tgctccagtc gagttgctgt
    18001 gcgtatttgt cgttggccag gatcgaatac agtgctttcg gagttgagcc gcccgagaga
    18061 gccacgagga aacgattccg ctcggcgatc gcctgcttgc ccacttccag gaagagacct
    18121 gcagcggcac gggtcagttc ctgggcatcg gtgaaggtgt ggattgctgg agcctgggac
    18181 atgtcaacgg tgagtgcggc gtttgggcag aggacgtttt tttgccgcgg gtttacgacg
    18241 accggctctg gccggtgtga gcatggcaat gaggcttcgg atggtgcgca cgggattggg
    18301 tccgagggcg attcggacgg ccggtcgttg atgggtctgc aacgattgaa ggtcgccgac
    18361 ggcttgcgcc tgtgccagcg tgccgaagga atagaacttt ccggggaccg gaaggtcggg
    18421 tttcatgctg tccacgagtt cgaggaacag gccgctgttc gggccacctt tgtggagttg
    18481 gcctgtggaa tgcaggtacc ggggaccata gcccgccgtg gtggcgatgt ggtggcgcag
    18541 catcaccgcc ttgcgcaacg cacgtattga ggcgtccagc tgagggctgg gagtggtgta
    18601 cgccagaatg gccaggtagc gattgggcgt cgcctgttcg agtaattgag tcagtgcctg
    18661 cttgggcgtg agcatcggca gtgaggggaa tcgtccctgt gtttcaactt cttttagcac
    18721 gcgtcctgtg ttgtctttgc tctcctgcac gttcggctga tcgaagggat ggatgccgag
    18781 cgcgtgtcct gcgaccgcgg tggcgaactc ccaacggaag aattccccgc cgaggtcgta
    18841 gcgatccgcc aggttcaatc gaaataccgg atgcccggcc cgttgcaacg tgatgatgga
    18901 tcgatccagc gcggcattgc ggtcgccttt gagtcgaagc gtgacgaaga gtcgatcggc
    18961 tccgtaggcg gtcggggagg ccaggggctc acctgccacg gggacgatcc cggttccttc
    19021 cttcccggtg ctttccgcga ggagttgttc cacccagagt ccaaatgaat tgagtgcggg
    19081 agaggtcagc agagtgacct tgtcccgccc tgccatggcc atcgcggcca tcatccctcc
    19141 cagggctgct cccgggttgt cttttgggtg agtggtgtgt cggcaggcct cacgcatggc
    19201 gagcgcccgg ctcaggagtt tgctcacgtc gagtcccagc agtgcggcgg gcacaaggcc
    19261 aaagtaggag aggacggagt atcgtccgcc gatgtccggt tgattggtga acgtacgcca
    19321 aaacccgcgc tcgcgcgcaa gctcttcaag tccggtgccg ggatccgtga tggcgacaaa
    19381 ctgggctccg ccgcgattgc ctttggtccg ccggacaagt tcccagaagt gagcgaagag
    19441 tgacatcact tcaatcgttc cgccggactt gctggccagg agaaagaggg ttcgcgccgg
    19501 ctgaatcgct ttcgtcacct gacggaccca gcctgggact gtggagtcca gtacccagag
    19561 tttgggggac cctttggccg aaccaaatgt gcagcggagg acttcgggcc ccaagctgct
    19621 tccacccatt ccgagaagca cgacgtcggt gatgcgagct tcccgggcag cctgctcgaa
    19681 gctccgtaac tcggcgagcc cgtcctgcat atggtcgagc accgtcagcc agcccagccg
    19741 attatcgatt tcagtcggac tgggcttcca gagagtatgg tctcgtgtcc agatacgctc
    19801 gatcgtatgg ctgctcgcca ggtcgtccag cgttttgctg atggttgggt tgtcgagcag
    19861 gagcgatgca gtgggacggg tcggcataac acgctctctt ccgttagtgg aggaacgaat
    19921 cgatggacct cggctctttc gtatcttagg ttcgagcgtg taaaaaggca aacaaagaga
    19981 agccagagac tgcgcaggag tttcttgaat gagtgggtgt gggtgctgtg cggttggagc
    20041 agtcgcgcgg aaggatgggg atctaggttt cttcttcctg atcacctaaa tcggccagta
    20101 cgagagaaat ggaattctgc gattccagtg cgccgacaat ggtgacaggt gttccaacag
    20161 aaacttggtc aaagagcaca gcaatctgag gattatccaa caggatgctg ccgtacggtt
    20221 gatccgtgat accttgtgcg atgccatgga gctggatcag gccgggcata tgctggctct
    20281 tgggaatcaa ccctgattgt tgagcttctt caaagcggcg tcgatcgtcg gcattgggat
    20341 agtcgagaat cagggcgcgg aaatacgggg tctgtcccga cccgcgtttg cggtgaatgc
    20401 gatattcgcc ttcgggcgtc gcgccgtcgc cgtagtgctg ttttgcccgg atccctctcg
    20461 accccaggcg gatcggatag gtcaatactt tcttgccgtt tttgtacaga gacagcactc
    20521 ggtcggcctt gctgatgaca atggcttgcg cacgatgggt gcgggaccat tcaatggttc
    20581 gttgggctga ttctcgccag gctgcaatga ggtcgtcatc ggcataccgg cccaactcgt
    20641 ttgtcagcaa ggcggtttgg gtgagaagta ttttccccgc acgatctgaa gcctggatgg
    20701 catgatcgaa ctgtccctgt tcaatgaaca ggcgagcttg tcgtacaagc agttcggttt
    20761 caaccggatg ttcgccgagt accacacggc tgccgatatc tcctacgcgg gtgttcaaga
    20821 ggcggagccg ttgctcgacc ttggcgagtt ttgtttcggc ggcggtgcgt tgtgcttgga
    20881 ggcgcgtgtt cacgtcggcc acgagctgcg acccttcgac ttgcaggctt tccaggtcgg
    20941 tctccagctc attttcttcc catggccaac ggacgatatt ttcctcggat tgaatgcggg
    21001 ctcgtaaaga gatccattcg cgggagaatt ttgaataggc ttcggggctg atgtcggatg
    21061 cccgcagcgt gacgaggtcg cggtcgatgc cctcaatggc ttcgaccagc tcgtcgggga
    21121 ccgtttcgac acacccgccc aggctcattg ccaggagtcc ggcaagggct agactgcaga
    21181 aggtgcggaa aatgtgggtc aggcgtctca tcgtttcatt ctatctcgca gggagcgttt
    21241 ctcgcgagct cttctcgttt ttgtcactga cacgttcgga caagcatggc catttcagaa
    21301 tgcgtacatc cggtttctgc tggggcatcc gcaggccgct aggaatacct ccaatccgtt
    21361 gtggtccacg accccgggct ctaattaacg caagctggat gccagggtgg agattgttgc
    21421 ggactggaac gattatcttt ccagaacaat tagttagatc gatgcggcaa gacgcgccct
    21481 ctgcctgttt ctgtggcatg tttgtcacag tcgggcaaga gtgcctcaag agttcgaggt
    21541 tgagtagcga tcgcgtggtc agaaaccttt ccccattcca gctctggttc tccttccgtg
    21601 cgcccttgca gggctgattg aagcaagcag gttgattgac accctcctgt cccgccccta
    21661 taatgcggcc tcttcattga agaatgatct ctcgacaagg agtgcgcaat gtcgttcact
    21721 tttaagcaac cttcgaatct caagcgcaag cgggaacatg gatttcggaa gcgcatgagc
    21781 accaagaacg gccgaaaagt tatcgctcgt cgtagagcca aaggccgcgc tcgtctcacc
    21841 gtctgattct ttcctgccgc taggcaatct gcaagtaggc aacatccagg tggtggatgg
    21901 tacacctggg gtgggtcctg tcgtcttagc ctggtcgtag taaaaatgcg gcgacgcaca
    21961 gtcttggcac gcggcctctg gtctttgagg cctggtctgg gctggtgacc cacaggcagg
    22021 tgtgttatgt cggcagggca aagggctgtt ccgtttttct tgaagcgtag ccgggatatc
    22081 cagcacatca agaagaacgg ccgacgggtt tcaacaggac tattcaatct tcagatttgt
    22141 ccgggtctgt cccaggatgc gatgttgggg attgtgatcg ggcgacggtt cgggacagca
    22201 gtgcgtcgga atcgggcaaa gcgtctgttt cgggaattgg gccgcgcggt gcgcccggca
    22261 ttagtcccag gtcacgcatt tctcgtgttt ccgaagcgag agtgcctgaa tctgccgttt
    22321 gcggagctgc agatcctatg gcgaggcaca ttacaaaggc agcggttggc ccgttttggg
    22381 gaaccctcgc tctgaggcgg ctgtgcattg tcctcttgat gatctatcga gcctgtctgt
    22441 ccccattgct cgggcctgcg tgccggttcc atcccacgtg ttccgtgtat gcccaggatg
    22501 ccatcgagcg gtatggcgtt ctcaaggggc ttctcatggc gggtaggcgc ttgctgaaat
    22561 gtcatccctt ccatcccggc ggagtggacc cggtacggta accgtcgtgg caataactct
    22621 tcagcttctg ttcagtacat aaattccatg gaaaaacgcg tcatcgtctt cctcatcgtc
    22681 tccctggccg tgattatcgg ttacgactat ctgctcaagg gcatggggct gttgccgccg
    22741 tctgagccgg tgcaggtggc tacgaaccca agccctgcgg ccgagacagg caagcctgcg
    22801 gggggtgcgg gagatccagc gaaggctgca agtgctgatc cggtctcctc cgtgaatggt
    22861 gcgcccgctt ctcccggcaa tgcgaccggg gcagcaagtg aggagcaaac ggttgaagtg
    22921 gacacggaac tgtttcgtgc caaattcagc aatcgcggcg ccgtgctcaa atcctgggaa
    22981 ttgaagcgat acagcgcggc tgtcgagcag agctcgacgc ctgtgcagtt ggtctatagc
    23041 ggaggacggt tcaagggacc tctgagcctg gtgaccgggg atcaagccgt gaccagcgac
    23101 cttggatctg ccctctatca ggtcacccag gatctctcgc atctcgatag cgcgcatcct
    23161 gtcggtcacc tcaccttccg gtaccacgat acccagaaga atgttgacgt tgagaaggag
    23221 ctgacctttc accatgggtc ctacgtggtg gacattgcga ttcgtactca agggctgacg
    23281 actccggtgg atgtcactct cggcaccaat ttcggcattg tggagtgggg ggaaggtttc
    23341 atcgggctca tggggtcggc gtcattggtc gacgacaagg tattgaagga aacgccggac
    23401 ggcgaggctg aacgcaaagg cgacgtgaag tggtcggcca ttcaggataa gtattt
//
LOCUS       DAOQRM010000022        22771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1494_length_22771_cov_16.413145, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000022 DAOQRM010000000
VERSION     DAOQRM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22771
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1494_length_22771_cov_16.413145"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1405
                     /locus_tag="PKK81_03565"
     CDS             18..>1405
                     /locus_tag="PKK81_03565"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNV31977.1"
                     /translation="MKILVGIGVVILLLIVLIVALPFLIDLNKYQDRYRPLIEEALNR
                     KVELQDIRLTIWPRIGARVGNFVVQDDPAFRTGPFASLSSLDVGVKLLPLLKGKVDVE
                     EIALRDPVITVLKNAQGQLNISTLGAKTPAPPAPSKPEAPAQAPGSPLQILALFAVDR
                     VSIDGGKLSYRDESTPKPTEYTVNNLEFLLTSVHLDESPTVHLGATVQPYNLPVRLDG
                     TFGPLVETLDVKSFTFNLALGKVAVGLKGRAVGGNLDATVNALQIDTADLPIALPLTK
                     PVQLKDVHLTLHALYPIPPDTPPANQVDLTDLGLTLVMGGSAINVKGTATKGLANLTA
                     ASASINSADLPVAIPLTKPVELKDLHVNLRAKYPPKEGAAPLELAEIPNLGLTVAMGS
                     SRIEVKGSVLGGLAKITANSKLINTTDLPIALTLKKPVEVKDLQAAAEMKGQEARLTN
                     ASLQLFGGFVRT"
     assembly_gap    1406..1505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1506..1988
                     /locus_tag="PKK81_03570"
     CDS             <1506..1988
                     /locus_tag="PKK81_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNV31978.1"
                     /translation="LQQASMLLKVVGVSLDNVKATAFSTIESDFAIKQGLIAVQRLLI
                     DSHDFQATGGGTIGLDQSLDMKLNLNLSQALSQKIAAGSPIARVALTGGRLSLPLLIT
                     GSTQAPSYGLDTKMFAGKVKEQVKEKVKGAVGDLLKGSAKPDDLKQQGKDLLKGLFGR
                     "
     gene            2007..2807
                     /locus_tag="PKK81_03575"
     CDS             2007..2807
                     /locus_tag="PKK81_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNV31979.1"
                     /translation="MSLANLVTQYIVQYGFRIMGAILVVVAALVVAKSVGRLFERWLN
                     EQDLEPPLRLLLLRLVKALVVILGLLIALDQLGLQIAPLVAGLGVAGLGMGLALQGVL
                     SNVVAGLSIIFTKPYRVGEHVSLLGVHGDVAKIDIFTTTLMHPDHSRIVIPNRKVVGE
                     ILHNFGTIRQLDLSIGVSYQTDIDTTLRVLRDLVTRHPKVLQDPAPMIGIAGFADSSI
                     TLSIRPWVQVAAVGTAHSELNQAIIQRLQADGVDIPFPQREVRLLNPS"
     gene            2940..3557
                     /locus_tag="PKK81_03580"
     CDS             2940..3557
                     /locus_tag="PKK81_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31980.1"
                     /translation="MSRQTDLDRPRRSERTSGSSLADTETESVLTEEHAAGTNLDKIR
                     DILFGAQVRDHERRFAKLEAQLLAEAAQLRNDLKERFASLEQYIRKEVDTVTGRLTAE
                     EQDRTDAVGRLTTELQTLAGVLQQTATQLREQSEQAHHDLRTQLQNQATSLSGDFTQQ
                     HAALSATLDDAVRQLSHQKTDRASLATLFQELSQRLSHDQPPQQA"
     gene            3674..5404
                     /locus_tag="PKK81_03585"
     CDS             3674..5404
                     /locus_tag="PKK81_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV31981.1"
                     /translation="MAHAPKAGTPAVERDYAELRSLLLAPEQSRLEQLQEQVEHLDLN
                     AHNLNRVLPEAIALRGEADHRLTHALTPHVSEALGVSVRKQPHMIVDAIAPIMMPAIR
                     QAIANALRSMVQSLNQTIEHSLSIRSMQWRLEALRTGKPFAEIVLLHTLCYRVEQVFL
                     IHAQTGLLLAHAAGDAVAVQDQTLVSGMLSAIRSFVQDSFGATPDQALNTLQVGDLTV
                     WIEQGPSAILAAVIRGTPPETFHVHLQDTLDRIHAEHSDALTRFIGDAAPFTGTTPLL
                     EECLRAQFETRRRAIAPITWVLLAAIVLAAIWWGVSAFQDRQHWQAYLDRLASEPGLV
                     VTSTHSAGNRYVLTGLRDPLAADPDVLLQDSGVAADRVEAKWSPYYALDAAFTLKRAG
                     IVLSPPDTVRLTLEGPRLTATGTASAEWIRQSRPLARLLPGITEYDDRRLVAQSLEAL
                     VQRMAGVWILFQQGTATIQSPEQLQGVRRISELLHQLDDTAHLSGATVILEITGQTDV
                     VGRSSRNQRLSEDRARSVLDALHPATFSAITFHARGIGPAPESSPSLGAATLPQDRRV
                     SFQATVHPAS"
     gene            5418..5954
                     /locus_tag="PKK81_03590"
     CDS             5418..5954
                     /locus_tag="PKK81_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HNV31982.1"
                     /translation="MIEKKICMLGAFAVGKTSLVRRFVTSCFSEQYQTTIGVTVDKKT
                     MSMDGQPVTLVLWDLYGEDEFQKLRRSYLRGSSAYLVVLDGMRRATLDIALHIQLTAA
                     DTLGPVPFVVLINKLDRQTDWEVTDQDLAQLAQRGWTVLLTSAKTGQGVEEAFTVLAR
                     AMLATSEQTPPAGTTDGA"
     gene            5944..7740
                     /locus_tag="PKK81_03595"
     CDS             5944..7740
                     /locus_tag="PKK81_03595"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV31983.1"
                     /translation="MAHNTHPSAPDLLCTDLLQRLQFAILEHVTGTEFRIIGHPPAWL
                     FTLYPDARHNRQLSVDARTPVLQNFLADAADAWQTDGDDIAHSGFWSEQTAAEEPWHF
                     HAMALRHGPSRLLLIQQSTAAYDQQATLLQRARDQVLQQHEHNRGHQRTQHELTTKLV
                     DMERSRDDVAVILQQLGLATLLIDQQGQVRFLSASAARLLDTPATGGPNGLLWETLLP
                     LTKPDRLALQSMLQQPAPRRERVQCHVETQAGRHLWLEIELQDDPRDTGTKIMFLHDM
                     TDVHHLRRLLELKAHYHDLVGKSRGMTQIYEQIQDLARVDSTVLIEGETGTGKELVAR
                     ALHQASARHKGPFIAANCAGLTDSLLGSQLFGHKKGAFTGAIDDQQGLFEAAQGGVLF
                     LDEIGDIPHNVQTNLLRVLQEKEVTRLGETRPRKVNVRVLTATHHNLSQDVAKGTFRA
                     DLLYRIRVSRIQLPPLRERKEDIPLLVASFLTEGRASMGKVIHRASPAAMAALMEYHW
                     PGNVRELKSTIECAMIHCKGDMLEATDLPPELRQGPMDSPSAVVVTGDERSRFVAALG
                     QARGNRTKAARLLGMSRATFYRRLSELDLPTS"
     gene            8008..8325
                     /locus_tag="PKK81_03600"
     CDS             8008..8325
                     /locus_tag="PKK81_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNV31984.1"
                     /translation="MDKLIPYSQPRPDAPTAREREILTHIWAGLTSQEIATRLQIAPK
                     TVESHRANLLRKFRATNSAQLLRLALLEGILHMPSSTSTGPSQTAADAQSTTAVRKLT
                     NLK"
     assembly_gap    8638..8737
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8857..9957
                     /locus_tag="PKK81_03605"
     CDS             8857..9957
                     /locus_tag="PKK81_03605"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNV31985.1"
                     /translation="MRRLGTLCFALLVMALAASIARAEAPAPADATPALDAVTLKDGT
                     VIYGQVLGMIADELHIKTSFGPTAGDDIIKIMWPNVAKLVVNRPVPFSLKEGTTVVGT
                     AQPGEPGTMILKAAPTGTPMAIPLDAVIGMNQPAVIYTGALQAGFSQTTGNSHLRNGS
                     LLGELSARSESLRLTILGRYIYGDNAQNLIVRNSRGTIKLDFFLSKRLYWFASAYFEQ
                     DTFQDLKLRTALATGPGYQLLDRGDLTGFFKDMTLWAEAGAAYFNEDFKLADDKSSTR
                     GRWAVKWNWPLWGGDQVSLYHFQEGFQSLANAKDLYLTADTGLRFKVWGGLVSGFQWT
                     MRYNKNPPPGVSDTDNLYLITLGYSFDTSRKQ"
     gene            10008..10433
                     /gene="mscL"
                     /locus_tag="PKK81_03610"
     CDS             10008..10433
                     /gene="mscL"
                     /locus_tag="PKK81_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNV31986.1"
                     /translation="MSMMSEFKEFAVKGNVLDMAVGVIIGGAFGKIVSSVVSDILMPP
                     IGLLMGKVDFSSLFIPLTEDAKGKSLAAAKAAGAATINYGVFLQTLLDFTILAFVIFM
                     VIKQMNRFKKTAPPGPPPAPPKEEALLTEIRDLLKNQRH"
     gene            10509..11711
                     /locus_tag="PKK81_03615"
     CDS             10509..11711
                     /locus_tag="PKK81_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV31987.1"
                     /translation="MTTLRTIPVLLSTLLLASCATAPSPNDSASRALNEMKAVAKPLP
                     PKPDPKDARIAELERQKAALEAELAQVRSSLTGDLDQAKARTSALESQLSQRDQELAS
                     LRNASGDKERLASQLSDVERQLSAKDQELAALRGSAGDKDRLAAQLAALQGQLSTKDQ
                     ELAGLKGNAGDRDRLSSELAQAKQRITELERQLEAKHHELAASKNAAGDREKIVADLA
                     AAKQRASDLESELARRDQEMASLKGALDQQKTSLAEAKDDLSKLLQAEVAKGNVTMKQ
                     LGDQLTLGLATTLLFDSGEATLKPGGADVLHRIGGVLKQYPDRSIHVAGHTDNVPIKG
                     RLAKTYPTNVELSQARAESARQALTEGGMAPDKIEAKGRADSRPIASNSTAEGRQKNR
                     RVEIVVGH"
     gene            11957..13462
                     /locus_tag="PKK81_03620"
     CDS             11957..13462
                     /locus_tag="PKK81_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV31988.1"
                     /translation="MRRAVWALMVLSSLAAGCETAPHVQPIASSPATVEPKLLALQQE
                     REQLLTTLGEFHDRIRDLESKLGDRQSQPAAASYDQLLSAKEAELVELRRLAPERDRL
                     TGQLTTAANELIQARQRIGTLEQQLATRDKDLAALQTRATAVADLEVARRRISELEAH
                     IARQDRDLHTVRTGSAERDSLAAQLQTATATIESLKARISALDQQLKDREQAYETVRS
                     RLMERDKLVPQYNAMIAEIYQARHRIAALEQRLNEKGRDLSPRQKGTQLTTSQRDGGP
                     LNQRSGAANQSLGDTAKSIPDGKSAPQGKDSTRSGVAFPGNPAQAEARSGSFAAVKEE
                     LLKVLPGDNRQKTISMRQDGNRLTVALDSNLLFTSGDAALSPEGATILKRIGAVLGQL
                     SDKFVQVAGHTDNQALSKTLQKTFPDNKALSWARAENARRALVNGGIPADRTKAVGLA
                     DTRPLTSNATEQGRQKNRRLELIIVQGPTVAATVEESRRGQVRLATLSATP"
     gene            complement(13527..14771)
                     /locus_tag="PKK81_03625"
     CDS             complement(13527..14771)
                     /locus_tag="PKK81_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV31989.1"
                     /translation="MNRISRRRFLQLSAMTGGALLFSDLADRVLGASSDHAVAFAAEP
                     IKIGILDPLSSPYKTSSIHDVHGANVAVDLFNKRGGVLGRPVVILEADDASNPETAIK
                     AATKFVQDDQVDVLMGTFNADCALVVSELAKKENKLFMVTGSLLPELTGAACSSHTFV
                     FMPNASMMAQAVVPHLVKAYGTRWYMLTTSSLDGKAMAQAMVTAGQAHGVEFVGETLV
                     PFGATDFTAAFTAAKDKHPTLVVLNLYGWDLVHALKAYTKLEFAKEKIGVGGMIAGEQ
                     IGRPLGYANNAGIWGLIWDPKVNTEGSRQFIRAVVDKYNHTPTSRCYLGYAAMTQILE
                     AIQRAGTTNAAALIKALEGREFDGLKEGRSYFQASNHQHMQDVLVGEAYGKELGLGHY
                     KILATVPADRLAAPVHAGVCQL"
     gene            complement(15126..16211)
                     /locus_tag="PKK81_03630"
     CDS             complement(15126..16211)
                     /locus_tag="PKK81_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV31990.1"
                     /translation="MTRSASFAAAIFLLLLLGIGSTAWAYRDYFSETQKAQLANIQTV
                     LVEVIALTDTGAGNPEGIREVVTRRMKEMGYTAIADPALPHDVVVRVKCEQRKTWEGT
                     AAAGGDNDLLDAPSRLWKGPACQVTYALGGMKIKWQKEVRTEFEDANQAAQSAQGGAP
                     GPYALTALQVELEKYEFPLFLTAEWGQPDRLLKLMDSPDTSQLRKLKIISLLGEMVAD
                     EALPHLTEALKDKDLAKQAAVALGNMGKEGIPVLIEILKHSKQPDLQAAAAKGLGDLG
                     NTHSDPRVVPPLLEMLDAPGIDIAVQTEIAWALGRVPDRRSVEPLFALDRKLQKIRND
                     PPDPQIKKLKEAVFWSIKQVYTEDQYS"
     gene            16539..16621
                     /locus_tag="PKK81_03635"
     tRNA            16539..16621
                     /locus_tag="PKK81_03635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16573..16575,aa:Leu,seq:tag)
     gene            16670..18007
                     /gene="tig"
                     /locus_tag="PKK81_03640"
     CDS             16670..18007
                     /gene="tig"
                     /locus_tag="PKK81_03640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNV31991.1"
                     /translation="MNSFMKMEMTELGPMKRALKIEVPADEVNLRFVQAYSELNRQVR
                     IPGFRPGKAPLQLLEKRYAKTIEEDVIRSLVPDYYDRAIRQAGIVPVLVEIPPLERVK
                     VKKDTPFSFTATVEIKPTIELRDYKAPNPISLKQDQRTVSDEQVHKALDVLREQMAQL
                     HPAPAGTTLADGDYAVLDIEGTLDGKPLEGTTKEGYLHKMGSHASVLGIDIESHLPGK
                     KEGEAITIPQAYPATHPDTRVAGKTVSFRCTIKSVKQKQLPALDDEFAKDCGPYQSLQ
                     EIREKLRTEMERALKKDIEDSYKDTILKRLAETHHFELPGTLVERELSAMVRQQLQSR
                     QRKSGDNPDTSPTAPQTEDAKKLQDELRPEAERRVKVGLILEALAAKESITVTNEDLG
                     NEITRLAAEVKLSVEEVTRMIQAGGQDTLDDLRSRILADKALDFVYKHAMIQG"
     gene            18135..18770
                     /gene="clpP"
                     /locus_tag="PKK81_03645"
     CDS             18135..18770
                     /gene="clpP"
                     /locus_tag="PKK81_03645"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002869062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNV31992.1"
                     /translation="MLVPIVIEQTNRGERAYDIYSRLLKDRIIFLGAPIDDVFANLII
                     AQLLFLEAEDPEKDINLYINSPGGSVTAGLGIYDTMQYVKPAINTICLGQAASMGAFL
                     LTAGTKGKRYALPNARVMIHQPMGGFQGQATEIDIHAREILKIRERLNEIMAKHTGQP
                     LDKISQDTERDYFMSSEEAKRYGLIDEVITRPLKSLKPVGSTDSGKDGGKS"
     gene            18797..20053
                     /gene="clpX"
                     /locus_tag="PKK81_03650"
     CDS             18797..20053
                     /gene="clpX"
                     /locus_tag="PKK81_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNV31993.1"
                     /translation="MAKQEKIDRHLRCSFCGKSRDEVRKLIAGPTVYICDECVNLCND
                     IIAEDWEEAKEEISSKLKKPAEIKHHLDQYVVGQDRAKRILSVAVHNHYKRISAKEKD
                     VDDVELQKGNILVIGPTGTGKTLLAQTLAKFLDVPFTLADATTLTEAGYVGEDVENII
                     LKLLQAADYDVERAERGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGTVA
                     NVPPQGGRKHPHQEFIQVNTSNILFVCGGAFVGLEHIIEQRMNRKSMGFGAEVRGRND
                     IRLGELLPHVQPEDLLKYGLIPEFIGRLPVVATLDELDEQALIRILTEPRNALTKQYE
                     KLLSFEKVKLRFTEGALSAVARKAFTQKTGARGLRSILEEAMLDVMYDAPSQKQIKEV
                     VITEDAISGKHAPIRIFEQDKDVKSA"
     gene            20270..20794
                     /locus_tag="PKK81_03655"
     CDS             20270..20794
                     /locus_tag="PKK81_03655"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV31994.1"
                     /translation="MKFPVVSSNRHKGKTLEIDSSKEVITLVGINGEPMGSLAWDFLV
                     DQILTYRKPQQSREARSEPRISLSIRVKYHTPEGTQFESRAGGIGGGGLFIESFTPLA
                     VGTKLAMEFTLPESPGEWLSAKGVIAWVCPKADQYTFSPGMGVRFSDIAAETRNRVLS
                     LVHSVKSITQDEPK"
     gene            20860..21378
                     /locus_tag="PKK81_03660"
     CDS             20860..21378
                     /locus_tag="PKK81_03660"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV31995.1"
                     /translation="MKFPVTATREHQGKTLSIDCDQETLSLFDDTGQRLSILTWGTLI
                     EHMLSVDEDARFAHSRAHPRAPLAIKVHCTTGDGKHFDSLTGGISGGGLFIESSAPLA
                     PGTELKVEFALPDRPSQKFETRAKVAWARSKPERYLLFPGMGIQFTDIDPEAQARLID
                     LVASLNRTRATA"
     gene            complement(22368..>22771)
                     /locus_tag="PKK81_03665"
     CDS             complement(22368..>22771)
                     /locus_tag="PKK81_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV31996.1"
                     /translation="MEERMAAPAPVMRAQYPPLPPGAQSKPVAKRPSAIVNKDAVARV
                     QAAQAAMRTKAAQAQPSGLSAQDMQGLGEGLGDAVRTFRQAVADVRSSVDPEMRTIRA
                     EMDSAQKELEQSIEAAKQAPVLDDDAPAKSV"
BASE COUNT         5251 a         7085 c         6079 g         4156 t
ORIGIN      
        1 ttcagcggag gtacgtgatg aaaatcctgg tcgggatcgg tgtcgtgatc ctgctgctca
       61 tcgtcctgat cgtcgcgtta ccattcctca tcgatctgaa caagtatcaa gaccggtacc
      121 gccccttaat cgaagaggcc ctcaaccgga aggtcgaact ccaggacatt cggctgacca
      181 tctggccacg catcggcgca agggtcggca acttcgtcgt gcaggacgac ccggcctttc
      241 gcacaggccc cttcgcatca ctctcttcac tggatgtcgg cgtcaagctg ctgccgctgc
      301 tcaagggcaa agtcgacgtc gaggagatcg ccctgcgcga cccggtcatc acggtcctga
      361 aaaatgccca gggccaactc aatatctcga ccctcggtgc caaaacaccg gctccgccgg
      421 ccccctcgaa gcctgaggcc cccgcgcagg cgcccgggag tcccctccaa atcctggcgc
      481 tctttgcggt ggaccgggtc tcgatcgacg ggggaaaact gtcgtatcgt gacgagtcta
      541 ccccgaaacc gaccgagtac accgtcaata atcttgagtt tctgctcacc tcggtccacc
      601 tcgacgaaag ccccaccgta catctgggtg cgacggtgca gccctacaac ttgccggtcc
      661 ggctcgatgg caccttcggt ccactcgttg aaaccctgga cgtgaagtcg ttcacgttca
      721 acctcgcact agggaaagtg gccgtcgggc tcaagggacg cgcggtcgga gggaacctgg
      781 acgccaccgt caatgcgctg cagatcgaca cggcggatct ccccatcgca ctgccgctga
      841 cgaaaccggt acagctcaag gatgtgcatc tcaccctcca tgccctgtac ccgattccgc
      901 ccgacacccc gcccgccaat caggtcgatc tgaccgatct gggattgacc ctggtcatgg
      961 gcggatcggc gatcaacgtc aaaggcacgg ccaccaaagg cttggctaat ctgacggcgg
     1021 catccgccag tatcaactcc gcggatcttc cggttgccat ccccctcacg aagccggtcg
     1081 aactcaaaga ccttcatgtg aacctccggg ccaaatatcc gcctaaggag ggagccgcgc
     1141 cgctcgaact tgccgagatt ccgaaccttg gcctcaccgt cgccatgggg agctcacgta
     1201 tcgaagtcaa aggatcggtg cttggcggat tggcgaaaat caccgccaac tcaaaactca
     1261 tcaacacgac tgacctacca atcgcgctga cactgaagaa gccggtggag gtcaaagacc
     1321 tgcaggccgc agccgaaatg aagggacagg aagcacgcct caccaacgcc tcgctccaac
     1381 tgttcggcgg attcgtccgc acgcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnttgca gcaggcctcg atgttgctca aagtggtcgg cgtttcctta gacaacgtca
     1561 aagccaccgc tttttccacc atcgaaagcg actttgcgat caaacaaggg ctgatcgccg
     1621 tccagcgact cctcatcgac agccatgatt ttcaggccac cggcggcggg acgatcggcc
     1681 tcgatcagtc gctcgacatg aaactgaatt tgaatctgtc ccaggccttg agccagaaga
     1741 ttgcggccgg ttctcccatc gccagagtcg cactgaccgg cgggcgtctc tcgctgccgc
     1801 tgctcattac cggcagcacg caggcccctt cctatgggct cgacacaaaa atgtttgcag
     1861 gaaaagtcaa agagcaggtg aaggaaaagg tcaaaggcgc cgtgggagac ctcttgaaag
     1921 gttccgcgaa accggacgat ctcaaacagc aaggcaagga cctcttgaag gggctcttcg
     1981 ggcggtaaca gttgcgggga ggacgcatgt cgttggccaa tctggtcact caatacatcg
     2041 tgcaatacgg ctttcgaatc atgggggcga tcctggtcgt cgtggccgcc ctggtcgtcg
     2101 ccaaatcggt ggggcggctc ttcgaacgct ggctgaacga gcaggacctg gagccaccgc
     2161 tccgcctgtt gctcctacgc ctcgtgaagg ccctcgtggt gatcctcggg cttctcatcg
     2221 cgctggatca actcggcctg caaatcgcgc cgctggtcgc cgggctcggc gtggccggct
     2281 tggggatggg cctcgccctg caaggcgtct tgagcaatgt cgtcgccggt ctgtcgatca
     2341 ttttcacgaa accctaccgg gtcggcgaac atgtgtcgct cttaggggtg cacggggatg
     2401 tcgcaaaaat cgatatcttc acgacgacgc tcatgcatcc ggatcactcc cggatcgtga
     2461 tccccaatcg caaagtcgtc ggagagattc ttcacaactt cggcacgatt cggcagctgg
     2521 atctctccat cggggtgtcc taccaaaccg atatcgacac cacactacgc gtgctgcgcg
     2581 acctcgtcac ccgacacccc aaagtacttc aggacccggc cccgatgatc gggattgccg
     2641 gcttcgccga ttcctccatt accctgtcca ttcggccctg ggtccaggtc gcggccgtag
     2701 gaacggctca cagcgaactc aaccaggcaa tcattcaacg attacaagct gatggggtgg
     2761 acatcccctt ccctcagcgt gaggtacgac tactgaatcc atcctaagga cagagccgct
     2821 gatcaccctc caggctgcac atccacgccg ccggtacccg gcctgacttg ttgaccccgg
     2881 cgcaacatgc tagtctaggc ccctttagtg atagaacgag cgaccaagga gaccccatca
     2941 tgagtaggca gacggacctc gatcgccccc gacgctcaga acgaacctct ggcagcagtc
     3001 tcgccgacac agaaacggaa tccgtgctga ccgaggagca tgcagccggc accaacctcg
     3061 acaagatccg cgacattctg ttcggcgccc aggtacgtga tcatgagcgc cggttcgcca
     3121 aactcgaagc tcagctgctg gcagaagcag cccaattgcg caacgacctc aaggaacgat
     3181 tcgcctcgct ggagcaatac attcgaaaag aagtggacac ggtgacgggc cgcctgactg
     3241 ccgaagaaca ggaccgcacc gacgcggtcg gacgcctgac cacggaactg cagacgctgg
     3301 ccggcgtgct ccagcagacg gcgactcagc tgcgggagca gagcgagcag gcgcatcacg
     3361 atttgcgcac ccagctccag aatcaggcaa catccctctc cggtgatttc acccagcaac
     3421 acgccgcctt atcggccacc ctcgacgatg cggtccgcca actctcacac caaaagaccg
     3481 accgcgcgtc ccttgcgacg ttgttccagg agctctcaca gaggctctcg catgaccagc
     3541 ctccgcagca ggcctaactc cacgccgatt cgtcagcggt tccggtctgc ccttcagacc
     3601 gcgcgtacgg ctgtttcccc gacaacgatg tgtcgcgtag aataccctcc ttccttcatg
     3661 ccacggagac gggatggcgc acgcacctaa agccggcacg ccggcagtcg aacgcgacta
     3721 tgccgaattg cgcagcctcc tcctggcgcc cgagcaatcg cgtctggagc aactgcagga
     3781 gcaggtcgaa catctcgacc tcaatgccca taatctgaat cgtgtgcttc ccgaggcgat
     3841 cgcgcttcgt ggggaggccg accaccggct gacccatgca ctcacccctc acgtctccga
     3901 agcgctgggg gtctccgtgc gcaaacagcc ccacatgatc gtcgatgcga ttgccccgat
     3961 catgatgccg gcgatccgcc aggccatcgc caatgccttg cgcagcatgg tgcaatcgct
     4021 caaccaaacc attgaacaca gcctctcgat ccgaagcatg cagtggcggc tggaggcctt
     4081 gcgtaccggc aaaccgttcg ccgaaatcgt cctgctccat accctctgtt atcgagtcga
     4141 gcaggtcttt ctcattcacg cacagaccgg cctgctgctc gcgcacgcag ccggcgacgc
     4201 ggtcgccgtg caggatcaga ccctggtctc gggcatgttg tcggcgattc ggagcttcgt
     4261 ccaggattcg ttcggcgcaa cgcccgatca ggcgttgaac accttgcaag tcggtgatct
     4321 gaccgtctgg atcgaacagg gcccctcggc aatcctcgcg gccgtcatac gtggcacgcc
     4381 accggagacg tttcacgttc acctccaaga cacgctcgat cgcattcacg ccgaacattc
     4441 cgatgcgctg acgcgcttca ttggtgatgc ggcacccttt accgggacca caccgctcct
     4501 ggaagaatgc ctgcgggccc agtttgaaac gcgccggcgt gccatcgctc ccattacgtg
     4561 ggtcctcctg gcggcgatcg tcctggcagc aatctggtgg ggagtgtcgg cctttcaaga
     4621 ccgccaacac tggcaggcct atctggaccg gctggcctcg gaaccgggcc tcgtcgtcac
     4681 ctccacgcat tcagcaggca atcggtatgt gctcaccggg ctgcgcgacc cgttggccgc
     4741 tgatccggac gtgttattgc aggacagtgg ggtggccgcc gatcgagtcg aagcgaaatg
     4801 gagcccctac tacgccctcg acgccgcgtt caccctgaaa cgagccggaa tcgtcctgtc
     4861 acccccagac acagttcgct tgaccctcga aggaccgcgc ctcacggcca ccggcacagc
     4921 cagcgcagag tggatccggc agagccgacc gctggcgcgc ctactccccg gtatcaccga
     4981 atatgacgac cgccgcctcg tcgctcaatc gcttgaggca ctggtccaac ggatggcagg
     5041 ggtctggatt ctgttccaac agggaacggc gacgatccaa tcaccggaac aacttcaggg
     5101 ggtgcgtcgc atatcagagc tcctgcatca gctggacgat acggcgcatc tctccggcgc
     5161 aacggtgata ctagagatca ccggccaaac cgacgtcgtg ggccggtcaa gtcggaacca
     5221 acggctgagc gaggatcgcg ctcggtcggt gctcgatgcc cttcatcccg ccaccttttc
     5281 cgcgatcacg tttcacgctc gcgggatcgg cccggcacct gaatcgtcac cctccctggg
     5341 tgccgccacg ctgccgcagg accgacgagt gtcttttcag gccactgttc accccgcttc
     5401 atgaagacgc ctctgacatg atcgaaaaaa aaatctgcat gctgggcgcc tttgccgtgg
     5461 gaaaaaccag tctggtgcgc cgcttcgtca ccagctgttt ctccgaacag tatcaaacga
     5521 ctatcggagt gaccgtcgac aagaaaacca tgtcgatgga cggccagccc gtgacgttgg
     5581 tgctctggga tctctacgga gaagacgaat tccaaaaatt gcgccgctcg tatctgcgcg
     5641 gatcgtctgc gtacctggta gtcctggacg gaatgcggcg ggccacgctg gacatcgcgt
     5701 tgcacattca actgaccgcg gcggacacac tcggcccggt tccgtttgtg gtcctcatca
     5761 ataaactcga ccgccagacg gattgggaag tgaccgacca agacctcgcg cagttagccc
     5821 aacggggttg gacggtgctc ctgaccagtg ccaagacagg gcagggtgtc gaagaggcct
     5881 tcacagttct cgcccgcgcc atgctcgcca catcagaaca aacacctccc gcgggaacaa
     5941 ccgatggcgc ataacaccca cccgtctgca ccggacctac tctgcacgga tctgttgcaa
     6001 cggctccaat ttgccatcct cgaacacgta accggtacag agttccgcat catcgggcac
     6061 ccgccggcct ggctattcac cctctacccc gatgcccgac acaatcggca actgtccgtc
     6121 gatgctcgca caccggtgct tcagaatttc ctggccgatg cagccgatgc atggcagaca
     6181 gacggcgacg acatcgcgca ctctgggttt tggagcgagc agaccgcggc cgaggagccc
     6241 tggcactttc atgccatggc gctgcgacac ggtcccagcc gtctcttgct gatccaacaa
     6301 agcacagcag cctatgacca acaggccacc ctcctccagc gggcccgcga ccaggtactt
     6361 cagcagcacg aacacaatcg tggacaccaa cgcacccagc acgagctcac cacgaaactg
     6421 gtggatatgg agcggtcgcg ggacgacgtc gccgtcattt tgcagcagct cggactggcc
     6481 accctgctca tcgaccaaca gggacaggtg cggtttctca gtgcctccgc agctcgcctg
     6541 ctggacacgc ccgcaaccgg cgggccgaac ggcctgctgt gggagacact gctcccgctg
     6601 acgaaaccgg accggctggc cctgcaatcg atgttgcagc aacccgcacc ccggcgcgaa
     6661 cgagtgcagt gccatgtcga aacccaggcc gggcggcacc tctggctgga gatcgaactg
     6721 caagacgatc cacgggacac cggaaccaaa atcatgtttc tccacgacat gaccgacgtc
     6781 caccacttgc ggcgcctgtt ggagctgaag gcgcactatc atgatttggt cggcaagagc
     6841 cgcgggatga cgcagatcta cgaacagatt caggatctgg cccgtgtcga ctccacggtc
     6901 ttgatcgaag gcgagaccgg tacggggaaa gaattggtgg cccgcgcgtt acaccaggcg
     6961 agcgcacgac ataaagggcc cttcattgcg gccaactgcg ccgggctcac ggattcgctg
     7021 ctgggcagcc aactcttcgg ccacaagaaa ggcgccttta ccggagccat cgacgaccag
     7081 caggggctct tcgaggccgc gcagggcggg gtcttgttcc tcgacgagat cggcgatatc
     7141 ccccacaacg tccaaaccaa cctgcttcgc gtactgcagg agaaggaagt cactcggctc
     7201 ggtgaaacca gaccgagaaa agtgaacgtg cgcgtgctga ccgcaaccca ccacaatttg
     7261 agtcaggacg tggcgaaagg aacctttcgc gccgaccttc tctatcggat tcgagtctca
     7321 cggatccagc tcccgccgct gagggagcga aaggaagata ttcccctcct cgtcgcctcc
     7381 ttcctcaccg aggggcgggc cagcatgggg aaagtcatcc atcgcgccag cccggcagcg
     7441 atggcggcac tgatggagta ccattggccc ggcaatgtgc gggaactcaa gagcacgatc
     7501 gagtgcgcaa tgattcattg caagggcgac atgctcgaag ccacagatct tcctcccgaa
     7561 cttcgtcagg ggccgatgga ctctccgtcc gcagtggttg tcaccggaga cgagcgcagt
     7621 cgtttcgtgg ccgccctcgg acaagcccgt ggcaaccgaa cgaaggccgc gcgcctcctc
     7681 ggaatgagcc gcgccacgtt ttaccgccga ctgagtgaac tcgatctccc aaccagctaa
     7741 cccgtcccgc agccactagc ccccactcac tgtctcgata tgtctcgcac gacacagcgc
     7801 gagacaacgg cgttcgtctc atcagcgcat aatcatggca gattcttaag ggtcctgagc
     7861 cgagacccca gatttcatcg tcgcgcggca aaacacacag cttatggcat agagcttgat
     7921 aggagagtac ccgcgaaaac cactgcgtgc aaaaccctga agacgggcgt cgcagcagaa
     7981 gtatcgaagc gatcgcaagg agaaccgatg gacaagctca taccctactc acaaccacgg
     8041 ccggatgcgc cgacggcaag agagcgcgaa atactcactc acatttgggc cggcctgacg
     8101 agtcaggaaa ttgcgacgcg gctacagatc gcccccaaaa ccgtggaatc acaccgtgcc
     8161 aatctcttga gaaaattccg cgcgacgaac tcggcacaac tgcttcgcct ggcactgctt
     8221 gagggcatac tccacatgcc gtcatcgact agcaccggcc catcccagac cgcggcggac
     8281 gcgcagagca ccacagcggt acggaagctg acgaacctca aatgacgctc tcagctgcca
     8341 cacccatgac tggatcaccg ttgcgcatgt gcacagcgac cgcaccggaa gtctcagact
     8401 cacaagcctg tcgcatagtg acccactcct ccgcgccatc ggccctgctg ggtccatgcg
     8461 ctcaccagac ggcatgacac tccgctgtct acgtatactc aacaggacga gccattcatg
     8521 cccgtggaaa ggctgtcctg cgctaggccc acaattcaag ccgaacagcg gcacgacgga
     8581 tttctcgcgc caaaatgttg atctcctacg cagaatcaat tatacatcgc gaacattnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntct cgcgccaaaa tgttgatctc
     8761 ctacgcagaa tcaattatac atcgcgaaca tttctcaccg atcaggcaac cgacacactg
     8821 ttgccgttcg caagccccag gaggaggacc taaacgatgc gacgacttgg aaccctctgt
     8881 tttgcgctac ttgtcatggc gctcgccgcc tcaatcgcgc gagcagaagc cccggcaccg
     8941 gccgatgcga cgcccgcgct cgatgccgtc acgctcaagg acggcacggt gatctatggg
     9001 caggtcctgg gcatgatcgc ggacgaactg cacatcaaaa ccagtttcgg gccgaccgcc
     9061 ggtgacgata tcatcaagat catgtggccg aacgtcgcca agctcgtggt caatcgtccc
     9121 gtccccttca gcctcaaaga aggcacgacg gtagtgggta cggcacaacc aggcgagccc
     9181 ggcaccatga tcctgaaagc cgcccccaca gggacaccga tggccattcc actcgatgcg
     9241 gtcatcggca tgaaccagcc tgccgtgatc tataccggcg ccctccaggc tggattttca
     9301 caaacgaccg gcaacagcca cctacgaaat ggcagcttgc tcggggaact gtcggctcgc
     9361 agtgaatcac tgcgcctgac gatcctcgga cggtacatct acggcgataa cgcccagaac
     9421 ctgatcgtac ggaacagccg cggcaccatc aagcttgact tcttcctctc gaaacgtttg
     9481 tattggttcg cgtcggcgta tttcgaacaa gataccttcc aagacttgaa gttgcgcacc
     9541 gccctcgcta ccggtccggg ctatcaactc ctcgaccgcg gcgacctgac aggcttcttc
     9601 aaggatatga ccctctgggc ggaagcgggc gccgcctact tcaacgaaga ttttaagctc
     9661 gccgacgata aatccagcac tcgaggtcga tgggccgtga aatggaattg gccgctctgg
     9721 ggcggcgacc aggtcagcct gtaccacttc caggaaggat tccaatcgct cgccaacgca
     9781 aaagatctgt acctgacggc cgacaccggc ctgcgattta aagtgtgggg cgggctcgtg
     9841 agcggctttc agtggaccat gcggtacaac aaaaacccgc cgccgggcgt ttcggatacg
     9901 gacaacctct acctgatcac gctgggatac agcttcgaca ccagccggaa acagtaaggg
     9961 ccgaaacggg ctcggcacag agtcggttca cacaaggagg tggcaggatg agcatgatga
    10021 gtgaatttaa agagttcgcc gtaaagggca atgtgctcga tatggccgtg ggagtcatca
    10081 tcggaggtgc gttcggaaaa atcgtatcgt ccgtcgtcag cgacatcctg atgccgccca
    10141 ttggtctcct aatggggaaa gtggatttct ccagcctctt tatccccttg accgaggatg
    10201 ccaaagggaa atcgctggcc gcagccaaag ccgccggtgc tgccaccatc aactacggcg
    10261 tcttcttgca aaccctgctc gattttacga ttctggcctt cgtcattttc atggtgatca
    10321 aacaaatgaa ccgcttcaag aaaacggccc ctccaggccc gccccctgcg ccgcccaagg
    10381 aagaagcgtt actgacggag attcgggatc tcttgaagaa tcaacgccac taattcgcgc
    10441 tcgcgcagga ctcatccccg ctgcctggga taaacccggc agctccgata tttttgaagg
    10501 aggattgcat gaccacactc cgtaccattc ctgtactgtt gagcaccctg ctgctcgcct
    10561 cgtgcgcaac ggccccgtcc cctaacgact cggccagccg agccttgaat gaaatgaaag
    10621 ccgtcgccaa accgctcccg cccaaaccag acccgaaaga tgcacgaatt gccgaactcg
    10681 agcggcagaa agcagctctc gaagcggaac tggcacaggt tcgttcttcc ctcaccggcg
    10741 atctggacca ggcaaaagcc cgaaccagtg cgctcgaatc acaactcagc cagcgcgacc
    10801 aggaattagc ctcacttcgg aacgcctcag gcgacaagga gcgactggcc agccagttgt
    10861 cggatgtcga acgtcagctg tcagcgaagg accaggagct cgcggccctc aggggcagcg
    10921 ccggagacaa ggaccgcctt gccgcacaac tggcggcact ccaggggcaa ctatccacca
    10981 aggatcagga gcttgccgga ctgaaaggga acgcgggaga tcgggaccgc ttgtcgtccg
    11041 agctcgcgca agccaaacaa cggatcactg aactggagcg acaactggag gccaagcatc
    11101 acgaactggc agcgtcgaaa aatgccgcag gcgaccggga aaaaatcgtt gcagacctcg
    11161 cagcggccaa acagcgggcc tcggacctcg agagtgagct cgcgcgcaga gaccaggaaa
    11221 tggcttccct caaaggcgcg ttggatcaac aaaaaaccag tctggcggaa gcaaaggacg
    11281 atttatcgaa actgctgcag gcggaagtgg ccaaaggcaa cgtgaccatg aagcagttgg
    11341 gagatcagct caccttgggg cttgccacca cgttgctgtt cgactccggc gaagcgacac
    11401 ttaagccggg gggagccgat gtcttgcacc gtatcggtgg cgtgctcaaa caataccccg
    11461 atcgttcaat tcatgtggcc ggccacaccg ataacgtgcc catcaagggc agactggcca
    11521 agacgtatcc aaccaatgtc gaactgtctc aagcgcgcgc cgaaagtgcg cggcaggcgc
    11581 tgaccgaggg gggcatggcc ccggacaaga tcgaagccaa agggcgtgcc gacagccggc
    11641 cgattgcgag caattcgacc gccgaaggac ggcagaagaa tcgacgcgtc gaaatcgtcg
    11701 tcggacacta accggctacc cgcagctcca ccagccttgc atcagcccca ggaacgcgat
    11761 agattgctgt cgtgttcttg gggcctgcat gtcactcaac agattctgtc gggacgcatg
    11821 gctcacacca actgatcatc atccgtcagc tcggtcacag tcgccttctt ttcttgactg
    11881 cggtggcttc tctcgggcga ctgtcgcccc catgcaaccg gcgccgtctt cctatcaatg
    11941 accggggaga gatcgtatga gacgagcagt atgggcgctc atggttctca gttccctcgc
    12001 cgcaggctgt gaaaccgctc cgcacgtcca gcccatcgca tcgagcccag cgaccgtaga
    12061 accgaagctg ctggcccttc aacaggagcg agaacagctg ttgaccacgc tgggtgaatt
    12121 ccacgatcgc attcgcgacc tcgaaagcaa actgggagac cgccagagtc aacccgccgc
    12181 ggcatcgtac gaccaattgc tcagcgcgaa ggaagcggaa cttgtcgaac tgaggcggtt
    12241 agcaccggaa cgcgatcgac tcaccggcca attgacgacc gccgccaacg agctcatcca
    12301 ggctcgccaa cgaatcggga cgcttgaaca acagctggcc acgcgagaca aagatctggc
    12361 tgccctccag acccgggcaa cggccgtcgc cgacctggaa gtcgcccgac gcaggatctc
    12421 ggagctagaa gcgcacatcg cccgtcaaga ccgcgatctc cacacagttc gtacggggtc
    12481 cgcagaacgg gacagcctgg cggctcaact acaaaccgcc accgccacca tcgaatcctt
    12541 aaaagcccgt atcagcgcac tcgatcagca actcaaagac cgcgagcagg cctatgaaac
    12601 ggttcgatcg cgcctcatgg aacgcgacaa gctagtgccc caatacaatg cgatgattgc
    12661 agaaatttac caggccagac accgcatcgc agcgctcgaa cagcgcctga acgagaaggg
    12721 gcgagatctg tcgccgcggc agaaggggac tcaactcacc accagtcagc gcgacggagg
    12781 accgctgaac cagcgctccg gcgctgccaa ccaatctctt ggcgacacgg ccaaatcgat
    12841 acccgatggg aaatcagccc cccagggaaa ggactccaca cgcagcggtg ttgcatttcc
    12901 aggaaacccc gctcaggctg aggctcggag cggaagcttt gcggcggtga aagaagaact
    12961 tctcaaagtc ctgccgggcg acaatagaca gaaaacgatc agcatgaggc aggatggaaa
    13021 tcgattaacg gtcgcgctgg acagcaactt gctgttcaca tcgggtgacg ctgcgctgag
    13081 cccggaaggc gcaactatcc tcaaacgcat cggtgcggtc ctgggacagc tttcagacaa
    13141 gttcgtgcag gtcgcaggac acaccgacaa tcaagcactc agcaaaacgc ttcaaaaaac
    13201 cttccccgac aataaggcgc tctcatgggc tcgcgctgaa aatgcccgca gggcactggt
    13261 caatggaggg atacccgccg acaggacgaa ggccgtcggg ctggccgata cacgcccgct
    13321 cacatccaat gccaccgagc agggcaggca gaaaaatcgc cgcctcgaat tgatcatcgt
    13381 ccaggggccg actgttgccg cgacggtcga ggagtcgagg cgcgggcagg ttcgactcgc
    13441 gaccctcagc gcaacacctt agcctgtcac acaaacggaa tcgccgcgta ctgacgactc
    13501 gccaccgtca catcacggcc tgttcgctac aactgacaga caccagcgtg aacaggcgcc
    13561 gcaaggcggt cggctggaac cgtcgccaga atcttgtaat gcccaagccc cagctccttt
    13621 ccgtaggctt ccccgaccag cacatcctgc atatgctgat ggtttgaggc ctggaagtag
    13681 gaccgtccct ctttcagccc gtcaaactcg cgcccctcca acgccttaat gagtgcggca
    13741 gcgttcgtcg ttcctgctcg ttgaatcgcc tcaaggatct gcgtcatggc ggcataccca
    13801 agatagcagc gcgaagttgg cgtgtgattg tatttgtcga cgactgctcg aataaactgc
    13861 ctggatcctt ccgtgttgac ctttggatcc cagatgagcc cccaaatgcc ggcgttgttg
    13921 gcataaccga ggggccgtcc aatttgttca cccgcaatca tcccacccac gccgatcttc
    13981 tccttcgcaa actcaagttt agtgtatgcc ttcagtgcgt ggactaaatc ccatccatag
    14041 agattgagca ccaccagcgt cggatgtttg tccttggcgg ccgtgaacgc cgcagtgaag
    14101 tccgtcgccc cgaaaggcac tagtgtctcc cccacaaact caacgccatg agcctggccg
    14161 gccgtcacca tggcctgcgc catggctttg ccgtccaatg agctggtcgt cagcatatac
    14221 cagcgggtac cgtaggcctt gaccaagtgg ggcacgacag cctgcgccat catcgaggca
    14281 ttcggcataa acacgaaggt atgcgagctg cacgcggctc cggtcagttc ggggagaagg
    14341 gagcccgtga ccatgaacag cttgttctcc ttcttcgcca actccgaaac cacaagcgca
    14401 caatccgcat taaacgtgcc catcaggaca tcaacctgat cgtcctgcac aaactttgtg
    14461 gcagctttga tcgccgtctc aggattcgag gcgtcatcgg cctcgagaat caccaccggc
    14521 cgccccaaca cgccaccacg tttattgaag agatcgacgg ccacattggc accatgaaca
    14581 tcatgaatgg aggaggtctt gtacgggctc gagagcggat cgagaattcc gatcttgatc
    14641 ggctctgcgg caaacgccac tgcgtgatcg gacgacgcac cgagcacacg gtcggccaaa
    14701 tcgctaaaaa gcagcgcgcc gccggtcatg gctgatagtt gcaagaaacg tctgcgtgag
    14761 atcctattca tacacttgat cccccctggt tttcattcgc cctcccaggc gccacgaccc
    14821 cgatggtaag gccatgatca caccgagagg gattgtctct cttaacagag tcatctatgt
    14881 tttcacagca cgaagttaga cctcggggac gacgagctcc tctcacaatg ggcaagatat
    14941 agccctcgcg agtgatgctc gtcagaacaa ctgggggagc cgggcaggaa tcttcctgga
    15001 gtgcggggga ttcacatatc cttcgctagt tgcggcacga ggacgaagcg ggactgagaa
    15061 cacgcatctc agcctccatc ccggtaaccg cggctggaat gtaccgggag agtgtggatg
    15121 acgggttagc tgtactggtc ttcggtgtag acctgcttaa tcgaccagaa tactgcctct
    15181 tttaacttct tgatctgagg gtctggggga tcgttgcgga tcttctgcag tttcctatcg
    15241 agtgcaaaca gtggctcaac cgatcgccgg tcgggaaccc gccccaatgc ccaggcaatc
    15301 tccgtctgaa cagcgatgtc aatgccaggc gcgtccaaca tctcaagcag cggcggcacc
    15361 actctcgggt cactatgggt gttcccaaga tcgcccaacc ccttagccgc cgctgcctgc
    15421 aagtcaggct gtttggagtg cttcaggatc tcgatgagca ctgggattcc ctccttcccc
    15481 atattgccca acgccaccgc tgcttgcttc gccaagtcct tatccttcag cgcctctgtc
    15541 agatgaggca atgcttcatc ggccaccatc tctcccagga gggaaataat cttcaatttc
    15601 cggagctggc tggtgtccgg agaatccatc aacttcagca gtcgatccgg ctgcccccat
    15661 tcggccgtca agaagagcgg aaattcatac ttctctaact cgacctggag cgcggtcagg
    15721 gcatagggac cgggcgcacc accctgagcc gattgagcgg cctgattggc atcttcaaat
    15781 tccgtccgaa cctccttctg ccatttgatc ttcatccctc ccaaagcata tgtcacctga
    15841 cacgccggtc ccttccacag gcgagaaggg gcatccagta aatcattgtc tccaccggcc
    15901 gccgcggtac cttcccaagt cttacgttgc tcgcacttta cccggacgac gacatcgtgc
    15961 ggaagggcgg gatccgcaat ggctgtatag cccatttcct tcatacgtcg cgtgacaact
    16021 tctctgattc cctcggggtt accagcgccc gtatcagtaa gcgcgatcac ctcaacgagg
    16081 accgtctgga tatttgccag ttgcgccttt tgcgtttcac taaagtagtc ccgataggcc
    16141 catgctgtag agccgatgcc aaggagaagc agaaggaaga tagctgcggc aaaggaagcg
    16201 ctccgcgtca tatggacttc ctcctttcca atagggtctt gatgaaggtc catgcaacaa
    16261 agaatcgaca cttcattata cgctccaagg cctcccgact ctacgaagga acgtcaagca
    16321 tcgacctgaa ccgacagccc acagtccccc gcccagccac gttatcctca gcatcctttt
    16381 tccactccga ccgacaggcc aacacgcagg ccccacctgc gtatgggttg acaggcagac
    16441 tcgttcgacg gtacaaaatg tcattattca tcgtgccttt ccacgcgcag gcacattcct
    16501 tatgtagcat cggtgagcaa cctagtactc agcgtcgcgc gagagtggcg gaactggcag
    16561 acgcgcgaga cttaggatct cgtgggtaac cgtgggggtt caagtccccc ctctcgcacc
    16621 acctctcaca gatccgtatg gcgcaaataa cttttcggac ccggaggacg tgaacagctt
    16681 tatgaaaatg gaaatgaccg aactcggccc catgaagcgg gccctcaaaa ttgaagtccc
    16741 ggcagatgaa gtgaacctgc gctttgttca ggcctactct gaattgaacc ggcaggttcg
    16801 cattcccgga ttcagacccg gcaaggctcc gctccagtta cttgagaagc gatatgcaaa
    16861 gacgatcgaa gaggacgtga ttcgcagcct ggtcccggat tattatgatc gagccatccg
    16921 ccaagcgggc atcgttccgg ttctcgtaga aattccgcca ctggaacggg tcaaagtcaa
    16981 aaaagacacg ccttttagct ttaccgcaac cgttgaaatc aagccgacca tcgagcttcg
    17041 agattacaaa gctccgaatc ctatctccct caaacaggat cagagaacgg tctcggatga
    17101 acaggtccac aaagccctcg acgttctccg cgagcaaatg gcccaattac atcctgctcc
    17161 cgcaggaaca acattggccg atggcgacta tgcggtctta gatattgaag gaaccttgga
    17221 tggcaagcca ctcgaaggaa cgaccaaaga agggtacctt cacaaaatgg gctcacatgc
    17281 ctccgtcctt ggcatcgaca ttgaatctca tctcccaggt aaaaaggaag gtgaggcgat
    17341 aactattcca caagcctatc ccgcgacaca ccctgatact cgcgtcgccg gcaagaccgt
    17401 gagctttcgc tgtaccatca agtccgtaaa gcagaaacag ctccccgcgc tagacgacga
    17461 gttcgcaaag gattgcggtc cctaccaatc attgcaggaa atccgagaga agcttcgcac
    17521 cgaaatggaa cgggccctga agaaagatat cgaggatagc tacaaggaca ccatcttaaa
    17581 gcgactggct gaaacccatc attttgaact tcccggaaca ctggttgagc gagagctgtc
    17641 ggccatggtc cggcaacaat tgcaatcccg ccagcggaaa tccggcgaca atcccgacac
    17701 ttcacctacc gcgcctcaaa cagaggacgc caagaaactc caggatgaac tgcgcccaga
    17761 ggccgaacgg cgcgtcaagg ttggacttat ccttgaggca ctggcggcga aggaaagcat
    17821 caccgtcacg aatgaggatc ttggcaacga aatcacgagg ctcgctgcgg aggtcaaact
    17881 ctcggtcgaa gaagtcaccc gcatgattca agccggtggg caagacaccc tagacgacct
    17941 tcgatctcgg attttggccg ataaagcttt ggattttgtc tacaagcacg caatgatcca
    18001 ggggtagcat agtcggctgc catgagttgc agaactggat aaccaggcgg tgatcaccaa
    18061 aatccaggtt aattgagtat ggtaaactga agagtttcca cctgtcaggc ctctagcgga
    18121 aaggattcag ctgaatgcta gttccgattg tcatcgaaca gacgaaccgc ggcgaacgtg
    18181 cctatgacat ttactcacgc ctgcttaaag accgcatcat ctttctcggc gcaccgatcg
    18241 atgacgtctt tgcaaacctg attatcgctc aactgctgtt cctggaagcc gaagaccctg
    18301 agaaggatat caatctgtac atcaattctc ccgggggaag tgtcacggct ggacttggca
    18361 tctacgatac gatgcaatac gtcaaacctg ccatcaatac gatctgctta ggccaagctg
    18421 ccagcatggg cgccttcctc ctgacagccg gcacaaaggg caagcgctac gctttgccga
    18481 atgcccgagt catgatccat cagccgatgg gaggatttca gggccaggcc acggagatcg
    18541 atattcacgc gcgcgagatc ctgaagattc gcgaacgcct gaacgaaatc atggctaaac
    18601 acaccgggca gccactcgac aaaatttcgc aggacaccga gcgagattat ttcatgtcga
    18661 gcgaagaagc aaagcggtat ggattgatcg atgaagttat cacacgcccc ctgaagagcc
    18721 tcaagcccgt agggtccacg gactcaggga aggacggcgg caagagctag ttgacctttg
    18781 gtgtacagga ggcagcatgg ctaagcaaga aaagattgat cggcacctgc gatgctcgtt
    18841 ttgcgggaaa agccgtgacg aagtgcgtaa gctcatcgcc ggtcccaccg tctacatttg
    18901 cgatgaatgt gtcaacctct gcaacgacat cattgcggag gattgggaag aggctaagga
    18961 agaaatatct tcgaagctga agaaacctgc cgaaatcaaa caccaccttg atcaatacgt
    19021 ggtggggcag gatcgtgcca agcgaatcct ctccgttgcc gtacacaacc actacaagcg
    19081 catctccgcc aaggagaaag acgtcgacga tgtcgaactc caaaaaggca atattctggt
    19141 catcggccca accggcaccg gaaaaacact cctggctcaa accctggcca agttccttga
    19201 cgtgccgttc acccttgcgg atgccaccac gctcacagaa gcaggctatg tcggtgaaga
    19261 cgttgagaac attatcctaa aactgcttca ggccgccgac tacgatgtcg aacgcgctga
    19321 gcgcgggatt gtatatatcg acgaaatcga caagattagt agaaaaagcg atagcccttc
    19381 catcacgagg gatgtctccg gtgaaggcgt tcagcaggcg ctcctgaaac ttattgaagg
    19441 aaccgtcgca aacgtgccgc cgcaaggtgg gcgcaagcac ccacatcaag agttcattca
    19501 ggtgaacacc tcaaacatct tgttcgtctg cggcggggca tttgtcgggc ttgagcatat
    19561 aatcgaacaa cgcatgaaca gaaaatcgat ggggttcgga gcggaagtcc gaggccgcaa
    19621 cgacatccga ttgggtgaat tgctgccgca tgtccaaccg gaggatctcc tgaagtatgg
    19681 attgatcccg gagttcatcg gccgcttgcc ggtcgtcgcc accctggatg aactcgacga
    19741 gcaggcgttg attcgcatcc ttaccgagcc ccgaaatgcg ctcaccaagc aatatgaaaa
    19801 gttgctttcc ttcgagaagg tcaagctccg ttttacagag ggagccctga gcgccgttgc
    19861 acggaaagcc ttcacacaaa agaccggtgc acgcgggtta cgctcaattc tagaagaggc
    19921 catgctcgac gtgatgtacg atgcgccttc gcagaaacaa attaaggaag tcgtcataac
    19981 cgaagacgct atcagcggaa aacacgctcc gatccggatc tttgagcagg ataaggacgt
    20041 gaagagcgca tagctttccc gtggcagttt attctcacca ctagcttact ccttccagag
    20101 ggggcctcgc gccccctctt tttatgctcc cccggggcag cacttcaggc gtgccttcac
    20161 caacccactc cccaaatctt aggtttttgt cgacaagacc atttcgtgcg gtatacttgc
    20221 ggaaagatgg atatcctccc actggtgcct tttccgaagg gcggcgcatg tgaaattccc
    20281 agtcgtatcc agcaatagac acaagggcaa aacgcttgag attgattcct ccaaagaagt
    20341 gatcaccttg gtgggcatca acggcgagcc aatgggaagc ctggcctggg actttctcgt
    20401 cgatcaaatt ctgacctacc ggaaacctca gcagtcccgt gaggcgcgat ctgagcctcg
    20461 catctcactc tcaatacggg tcaaatacca cacccccgag ggcactcaat tcgaaagtcg
    20521 agcgggcggg atcggcggag gaggcttatt tatcgagagc tttacgccct tagccgtggg
    20581 aactaagctc gccatggagt ttactctgcc ggaaagtccg ggcgagtggt tgtcggccaa
    20641 aggcgtgatc gcgtgggttt gtccgaaagc cgaccaatat acatttagcc cgggaatggg
    20701 cgtccgcttc agcgacatcg cggcggaaac acggaatcga gtactcagcc tggttcattc
    20761 cgtcaagagc atcacccagg acgaaccaaa gtaaacaact cctatacaac accgcaattc
    20821 gaaactcccc aatacacaca caagagatct gagccaagga tgaagtttcc cgttaccgcc
    20881 actcgcgaac accaagggaa gacgctcagc attgactgcg accaagaaac cctgtctcta
    20941 ttcgatgaca ccggccaacg cctcagcata cttacctggg gcacgttgat cgaacacatg
    21001 ctctcggtcg acgaagacgc acgcttcgcc catagccgtg cgcatccccg agcgcccctc
    21061 gcaatcaagg tacactgcac caccggcgat ggaaaacact tcgacagtct gacgggcggg
    21121 atcagcggag gcggactatt tattgaaagt agtgcaccgt tagcacccgg cactgaattg
    21181 aaggttgaat ttgccttgcc cgaccggcca agtcagaaat tcgaaacgcg cgcgaaggtg
    21241 gcatgggcac gttcaaaacc cgaacgatac ttattattcc ccggaatggg cattcagttc
    21301 accgatatcg acccggaagc acaagcacgg ctcattgact tggttgcctc tctgaaccga
    21361 actcgtgcga cagcgtaatc tgttgcacag cccatcgagc ccatccacga accatcatat
    21421 ctggatccgc ctcagccgcc ctcgacagct ccgccaagac cccgttatca ccgattttgc
    21481 ctaaccgaaa cgcggcctct gcgcgaacgc ttgccgcccc atctgtcctg agaagtgagc
    21541 gaaggtggga aacggcacca ggattcccgc tctcccctag cacggccgcg actccctgac
    21601 gcacacgcgc atccggatct cgaagcgctt tgatcagatc agcggagagc gacacggtat
    21661 ccaggctgag caacgcacga gttgcagcaa tacgagcaga accctgcgga tgccgaagaa
    21721 ccgcaagagt ctgagcctca cgatccgcag aaaactcaat ttcaccgaga gccagcgccg
    21781 atgccattcg aaccggctca gcaaaatcgg ccaaatgccc tgcgagcgcc atcccactct
    21841 tcccgtcacc cagccgaccc agtgcgagag ccgcagcttc gcggacacga gcgtccggat
    21901 cattgagcgc cacgatcaga ctggcattgg aagacttatg gccgaccttc cccaaagcct
    21961 ctgcggcagt gcgccggacg tccgagtctg ggtcggcaag caactcaacg aggcgccgcg
    22021 ataccgtctc cggagccccc gatggagtat cgacgtaaca cccatacacc acggagcaca
    22081 gcagcagcca gaagaagaat tggagtcgaa aataagaacg catcggggaa gccgaacgta
    22141 tatgagtgag agaattcaac ggaattgaaa cgtctatcct cgcgggcaga aaagaaccta
    22201 agcccgcacg taactacgaa attggaatgc aggttcgaga tgagacactt gggtggatcg
    22261 cccaggatac caaccagcaa gacgcaagca aaaaagaacc ggtcgacttc ctaattcgac
    22321 gaggccccgg aatcacccgc gcaggggaaa agaattatta gcttctccta caccgacttt
    22381 gcgggggcat cgtcatccaa cacaggtgcc tgcttggctg cctcgatgga ctgttctagt
    22441 tccttctgcg ccgaatccat ttcagcccgg attgttcgca tttcgggatc gaccgaactt
    22501 cgcacatccg caaccgcctg acggaaggtg cgcacggcat cccccaatcc ctcccccaaa
    22561 ccctgcatgt cctgggctga caggcccgac ggttgagcct gagctgcctt tgttcgcatg
    22621 gctgcctgag ccgcctgcac ccgcgccacg gcatccttgt tcacaatcgc cgacggacgc
    22681 ttagccaccg gcttgctctg ggcgcctggc ggaaggggcg gatactgcgc gcgcatcact
    22741 ggggcaggag ccgccatacg ctcttccatc g
//
LOCUS       DAOQRM010000023        22244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1552_length_22244_cov_17.628374, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000023 DAOQRM010000000
VERSION     DAOQRM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22244
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1552_length_22244_cov_17.628374"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKK81_03670"
     CDS             complement(<1..815)
                     /locus_tag="PKK81_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNV31997.1"
                     /translation="MNERKSRFSSLSGLDIERVYSSDHLKDWNVERDLGQPGSFPYTR
                     GIYPSMYRSRLWTMRQFAGFGSADDTNRRFKYLLAQGQTGLSVAFDLPTLMGLDADDP
                     MARGEVGHCGVAISSLTDMERLFDGIPLDQVTTSMTINGPAAVLFAMYLAVAEKRGIP
                     FDRLGGTIQNDILKEYIAQKEWLFPPEPHIAIIVDTIAYCAAHVPKWHPISISGYHIR
                     EAGSTAVQELAFTLYDGLTYVDAAVKSGLDVDAFAPQLSFFFNVHNDFFEEIA"
     gene            complement(846..1727)
                     /locus_tag="PKK81_03675"
     CDS             complement(846..1727)
                     /locus_tag="PKK81_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNV31998.1"
                     /translation="MTIDDIKTIGVVGAGQMGRGIAQVLATAGWNVLLIDATEEALED
                     AIKKIWQGVTKAVEKGALRGDQAGSAMAIIHPMKHMQRLREAQVVIEAIPEDPVMKRA
                     LFAQLGQVCEPSTLLASNTSSISIASLGMASGRPDRVVGIHFMNPVPVMRLVEVVRAI
                     ETSEHTVQLALELVRRAGKTAVVCKDSPGFIVNRVLIPMINEAIFALEEGVATAEAID
                     LAMVEGTNHPVGPLALADRIGLDTVLAICEVLHQDLGDPKFRPCPLLRKYVEAGRLGR
                     KSGHGFYVYGDQPVTVS"
     gene            complement(1741..2925)
                     /locus_tag="PKK81_03680"
     CDS             complement(1741..2925)
                     /locus_tag="PKK81_03680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNV31999.1"
                     /translation="MNARHHAVIVSAVRTPMGSFNGSYSSVPATKLGSLAIAEAVKRL
                     QLPGDRIDEVLMGCVLSAGLGQAPARQAAIGAGLPHSVGAVTVNKVCGSSLQAVMMAT
                     RAIALGEANVMVAGGMENMTRAPFLLEKARQGYRLGHGELTDSLIKDGLWDVYNQFHM
                     GNAGELCAAKYRLSRQDVDDFALESYSRARDAIARGAFKKEIVPVEVPQKKGASVLVS
                     DDEEPNRVDLTKLRQLKPVFQDDGVLTVGNSPSCNDGAAALVIMGEEEAARLGLTPMA
                     RIVGYAGAALAPEWFTIAPVEAIQRLLKQTGLSIGDIDLFEINEAFSAVSLAINRELG
                     LDPKKVNVNGGAVALGHPIGVTGARIVTTLLYAMEARDAHRGLAALCIGGGEALALIV
                     ER"
     gene            complement(2922..4079)
                     /locus_tag="PKK81_03685"
     CDS             complement(2922..4079)
                     /locus_tag="PKK81_03685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNV32000.1"
                     /translation="MATDIVMPQLGESIAEGTVVKWLIPQGGAVEKDQPLLEVETEKV
                     ALDIPSPATGFLTEVLVPEGTTVPVGTMLARLDTQPASGVVNRVGGVTVRPMEAAQGE
                     PHYSPAVRQLAKEHGLDLSAITGSGEGGRVTKHDLLNVISKRESTGSVQGALGTTPSP
                     PPASTLEEIVPLSQMRKTIAERMVQSRHTAAHVATFFEADFTGIAGFREGRALTYLPF
                     VISAVTRAIREQPLLNSSWREQGLVMKNDMHIGIAVALDDGLLVPVIRHADRKGLNQI
                     AHEITDLAERARSKRLRPEEVQDGTFTITNHGGFGSLFSTPIINQPQTAILGVGSVQK
                     RAVVINDAIAIRPMCYLSLSFDHRVIDGATADRFMARVKQHLEESGWEKLL"
     gene            complement(4184..5176)
                     /locus_tag="PKK81_03690"
     CDS             complement(4184..5176)
                     /locus_tag="PKK81_03690"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNV32001.1"
                     /translation="MTTATLTQEVTYVDAISQALDEEMSRDERVFLMGEDIGAYGGAF
                     KVTEGFLKKYGEWRVLDTPISESGIVGAAIGAAMMGLRPVVEMQFADFISCAFDQITE
                     VAAKNHYRWGAAVPLVIRAPFGGGVHGGPFHSECPEGWFFHSPGLKIVAPSTPYDAKG
                     LLKAAIRDPNPVLYFEHKFLYRRIKAALPQEDYIVPLGKAEVKRTGRDVSLITYGAMV
                     HLALEAAELLGKEGIDLEVVDLRTLIPLDKETIYASVRKTSKVVLLHEDNKTGGIGAE
                     ISALLAEDCFDSLDGPILRIAPPDTPVPFSTPLEEFFLPKVSDIVAGAKKLAAY"
     gene            complement(5173..6174)
                     /locus_tag="PKK81_03695"
     CDS             complement(5173..6174)
                     /locus_tag="PKK81_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNV32002.1"
                     /translation="MDVAVIANEIKRGDLLDMYYYLRLTRSLEDRISALYRQGRIVGG
                     VYTSHGMEAIAVGYASALRPDDVIAPFHRDMGAFLIRGFSPGEIIAQYLGKQGGPTKG
                     KDGNVHMGDLKRGMIGFVSHLADNMPVAAGAALAFKIRGESRVAFAGTGDGGTSRGDF
                     HEAMNFAAVRRLPVVFFCTNNQYAYSTPVRYQMAITDVVERAKAYGMPGEIVDGNDVA
                     AVYLASQRAIAKARAGDGPTFLEFKTMRMHGHSEHDAAKYVPRELLEEWKTKDPILRA
                     ERLVTQLGYGDESYFHEVGERAKKEVDAGTEFAEQSPLPEGRETLVGVFAIEDEVHP"
     gene            complement(6205..6966)
                     /locus_tag="PKK81_03700"
     CDS             complement(6205..6966)
                     /locus_tag="PKK81_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32003.1"
                     /translation="MMPSIDKSPETATLELVERQRKLYEGSEIDVRLEHLIRDTPHPT
                     LSSWDIQRLLHDSRAVYFDSHVEVVSGHHTDIYLRFESIARFPDLVTIIASNMAEWIL
                     QTFRHHPPTGILTTSSDARFLAERTSDILAPQMPLRVVLTPFNHETGKIGTDIIQGTV
                     ETGERFLSLNDVTTRGLCVSKLGKVVTDRGGQLAGMMVFARRDSGQFPLMDELTARFP
                     FYFSVDLDMPQWDASDCHSCKDHKPLLSWRDLPAL"
     gene            complement(6980..7963)
                     /locus_tag="PKK81_03705"
     CDS             complement(6980..7963)
                     /locus_tag="PKK81_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNV32004.1"
                     /translation="MKLVSFQVRTPVGTFTRIGALHNASIVDLNMAQARRLTDQGETQ
                     PRRLADAQVPATMLEFLEGGPAATDAARRAFDYAVGHGSSIAGPAGETIFYDPASVRL
                     TAPLPNPSSLRDFIAFEEHIAATSKRRGQPIPPEWYKAPVYYKGNHRTIIGPDHELRW
                     PLNTQKLDYELELACVIGRKGIDIAERDAVNYIAGYTIMNDFSARDIQFQEMACRLGP
                     AKGKDFATAIGPCIVTPDDIQDLASLRMIARINGEVWSEGRFGSIHWSFSQMIEHVSR
                     GEFLYPGDLFGSGTVGGGCGLELDRYLKPGDVVELEILPIGILRTTIAAQN"
     gene            complement(7960..9075)
                     /locus_tag="PKK81_03710"
     CDS             complement(7960..9075)
                     /locus_tag="PKK81_03710"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNV32005.1"
                     /translation="MYLVKKGAPPNQAHVGVPDGLHEEEHGRQGFSGPSSHLYHHHPP
                     TAWSRIDGPLRPRAFTCTELPTADYHSADGRPTLVLQSPDARVSLSRRAETMPHFVRN
                     ADGDEIVFVHRGRGRWETDYGFLSYEPGDYLVIPKGTTYRVHVDTKEEPGLFLMVETT
                     APVEVPDRGPLGRHALFDPGVLTAPELALPPEDAHSRHEWEVRIKRDGDYTRVYYPFY
                     PMDVAAWKGDLWVAKLNVRDFRPVISPRYHLPPSVHMTFQAGGLLISTFAPRPLESDP
                     SALRVPFYHRNMDYDEVLFYHQGDFFSRAGIHPGMFTLHPQGIHHGPHPQAIAASKTK
                     EQTDEVAVMIESRRAFTVMPECDAVELKDYAMSWSRP"
     gene            complement(9113..9709)
                     /locus_tag="PKK81_03715"
     CDS             complement(9113..9709)
                     /locus_tag="PKK81_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV32006.1"
                     /translation="MKEAIGIDHITLCVKNLAAVESLFTTVLGFHVIWSAQDVGSDMS
                     SMDTVVVQRGDAKIALMQGRNKARRSQICDFIDKYGEGVQHIALEVDDIEAVCREWES
                     HGVRFSGETKNGRDGFGPLKQRFTYPLFPGCGLFLELTQRQHGLEESKTFVRATVEAL
                     YRDIERDQTRGIENTIIDYETLPLPFKDTPSESFQSTS"
     gene            9935..10702
                     /locus_tag="PKK81_03720"
     CDS             9935..10702
                     /locus_tag="PKK81_03720"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNV32007.1"
                     /translation="MSFYEHVRADVLRHGAINNTYLTRFQAGEVTDREFHEFAVEFYS
                     FARFFPRILAAQLVNTEDEAVADELTKVLYSELGDGIVKHRHELLYRNFLRSIGIDIH
                     DAMHTPMKPSTRAYIQGMERLYSSRHHATALGASFGLENMAITMWDHLIPGLTRLKQV
                     RYPRMDMTYFTFHRQLESAHEKAMEHAVEAVGGAGANGCATMSAQEHRDFCLGMNAVL
                     DYLEGFWMGLERKASGIRRSRPQPARQLASELRGQSH"
     gene            10727..12442
                     /locus_tag="PKK81_03725"
     CDS             10727..12442
                     /locus_tag="PKK81_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNV32008.1"
                     /translation="MERVWISRYDAGVPLSSAYPEWTVPDLLRRSASCFPESTALFFY
                     GARLSFRELNDLTTRFAHGLLRLGVKRGDRVALMLPNIPQAVIAYYGVLKAGAIVAPM
                     NPLYVEREIHSQLADAGSEIIVALDLFYPRIQAVREQTGLPERIIITSLGDFLPTVKR
                     LLYPLKARLAKRWVAVEKTPPVYDFLALLDAASPRTEDDSPLLPTVEPDALAQIQYTG
                     GTTGTPKGVMLSHRNVVVNAMQGRLWCSDFQEGKEVFLGAVPFFHCYGLSTCQNLAVA
                     TGSQIVLLPRFHAEEAVKLIHQHRVTIVSGVPVMFAMITDCPKVGRYDLHSIRVCLCG
                     ASPLPTEVQERFERLSGVKISEGYGLTEAGPTTHCNPIQGDHPQGSMGLPFPDTDARI
                     VDEETGMRDIPTGEAGELIIRGPQVMQGYWRKTEDTQAVLRDGWLYTGDIVTRDEHGF
                     FFFLDRKKDVIKPWGETVYPREVEEILYQHPAVREAVVVGAPDHHYGEAVKAFVVQKE
                     GCAVTERELIEHCRRSLARFKVPVAIEFRTELPRTIIGKVLRRALRDEADPVPAGMQS
                     SRKAM"
     gene            12461..13651
                     /locus_tag="PKK81_03730"
     CDS             12461..13651
                     /locus_tag="PKK81_03730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNV32009.1"
                     /translation="MREVVIVAGVRTPIGNFGGALKDLPPHKMGELVVREAVSRAKVD
                     PRLIDEVIVGSVGHTSDAYNVARAIALMAGLPVRTPAYSVQRNCSSGLQPFVNAYQNI
                     QSEDADAQVVGGVESMSRAPFVSRDMRWGKRLRNAELIDSIWEGLTDAFCGQLMGRTA
                     ENLAEEFGIGREEQDRYAVESHRRAFKAIREGRLKDEILPLMVPKSVAGRDVAPVVVS
                     QDEGPNVGLTEQQLALYPPLFKEQGSVTAGNSCPLNDGAAAAVVMSSARARELGCRPL
                     GRIRGYAFIGVEPTRMGIGPAEALPLALKRAGVTLTDLELIEVNEAFAVQYLAVERVL
                     GLKRELVNVNGGAIALGHPVGMTGARLVITILYEMQRRGAALGAVALCVGGGQGAAMV
                     LERM"
     gene            13683..14906
                     /locus_tag="PKK81_03735"
     CDS             13683..14906
                     /locus_tag="PKK81_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNV32010.1"
                     /translation="MYIYKVGVVGAGTMGAQIAEVVSYAGVPVLLADIQESLARRGVE
                     SVRAIYQARVDKGKMTPEQLEEKMLLVTASPNLEALHDVDLVIEAVSEEVTLKQRVFR
                     ELDRVCARSAILASNTSALSISAIGASTTRPGKVLGLHFFNPAYAMPLVEVIPGLATD
                     PQTVDDVVGFAESLRKTPVIVKECAGFLVNRLLSPYLNEAVWCFQDGDVSIKEIDQDM
                     VAFGMPVGPFMLLDTVGLDIAFEVARILHRSYGPRMAPAPLLEALVKAGRWGIKSGRG
                     FYEYSEGEERSGDQDLDTLLQQVRQGGGQTRMEWTRLRPLLAMVNEAVTAYQEGVASA
                     RDIDLAMVAGTGFPNEKGGPLHVADQLGIDHVLHELEALRDTLGVRFWPAPMLRRMVD
                     AGFTGQLAGRGFFAY"
     gene            14999..15787
                     /locus_tag="PKK81_03740"
     CDS             14999..15787
                     /locus_tag="PKK81_03740"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNV32011.1"
                     /translation="MTTTAGSMLSCTVEGAVATLLINRPPANTLTPELLAELSTTFDQ
                     VAKDEAVKVVVLTGTGRFFIAGADIRVLASIASSKEGEAMALQGQAILDRIEGVGKPV
                     IAAINGICLGGGLELALCCHIRLAAEGSRLGQPEINLGIMPGFGGTQRLARIIGQSKA
                     MELILTGEPISAQEAKTIGLVSQVVSPEDLLRQAQGLARTIASKGQMAVRASLRAIRQ
                     GVELNLHDALALEARLFGELCDTADRREGLSAFLEKRQPHFMDR"
     gene            15825..17045
                     /locus_tag="PKK81_03745"
     CDS             15825..17045
                     /locus_tag="PKK81_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV32012.1"
                     /translation="MAPTFSKRALALGGGGFTGYLFEIGALTALDDLFEDGVTMNDLD
                     LYVGVSAGAAAASLLANGVRPQEILDTNLSGTRPYYFDHRNVFSPAIGEGLKTVWRVT
                     RQLVPLLKLYVRHYGEMTFIDLLDKAQDALPSGIYTLEPFAKYLEAIFKVKGLSDRFA
                     GLAKELYIPAIDLETGDGVLFGDEDWRDVPISRAVTASSAVPIYFCPVRIQGRDYIDA
                     GIGRMAFFEIAVQKHVDFMIMINPMARAPQTRSRQAQPAVGKPGNRLRDRGFLSIGEQ
                     ASRINFDARFSQALERFQQDYPEKEVLAISPVEADALLFERSFLSYDDRIHLLRAGYV
                     AVMTLARDRWEDLSAQFARHGLVMSRARFEDRAGRRLARLDEVARFADPNTLQPMVGP
                     ARIGGIGAAWRKAK"
     gene            17066..19003
                     /locus_tag="PKK81_03750"
     CDS             17066..19003
                     /locus_tag="PKK81_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV32013.1"
                     /translation="MTNGAEMFKGSLAAAEAARDTRAYSGLIAGLFEGVVRRSLFTSA
                     SIPAMSGDAKEFLDTLRLLLIEKVDPEAIDREGAIGEEVIEAFRAIGAFGIKIPRSYG
                     GLGLSQSEYHTVATLLGSHDAATSVLLSAHNSIGVAEPVKLVGNREQQQRLLPRLARG
                     DISGFALTEKSAGCDIWDLKTYAIPVHEGGVLVGYRLTGEKLYTTNAPREDHEFLASL
                     LVVIAQIVTVPQDVDRPKEERRFGAFVVETQSAGCRCTRLSFMGVRGIYNGQVHLRDV
                     FVPVADRLGEEGEGLRRALESLTVGRLTLPAACLGSLKQCLWLARARAQQRIQYDRPI
                     GEHTDIGSKIVLMASRVLALEAIVKITGIWTDAKVDVRLESAAAKVLATEWLLESLLD
                     LFRIYGGRAFETPDSLRLHGDLPVPIERMIRDALINVIWEGSNGILTLWIAREGLGEY
                     VTQGRAFLERGIEDMAQAVPFFARISARAFNPIAFLGRGVRSGADAAPWERFVFEESR
                     ALARKSLWASVRHRQGLARNQRLLRRLVTAGLQLFAVEALLWYGSQPEVREHALSRAL
                     VEDFCLRVKETFHPTPLLSLTTPLWDDDRRLFHLAKDILAGKAGWLEEGIIPSCPGGR
                     TGVAEPAGVVTGASVETWSGI"
     gene            19058..20041
                     /locus_tag="PKK81_03755"
     CDS             19058..20041
                     /locus_tag="PKK81_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32014.1"
                     /translation="MTEISGYFEAIKDRYALTSDYTLAEKLGIAQPDANLMRRGLKVP
                     KPELCIKIAKLLDRNPVELLLIAQKDKAPKQAKEYWTLALTAVDVMLHVPKRPRYLPK
                     KVEAIGRELKQLESQTLTYEGAAANAEAVRLMETAEQSVDAMMERWNIWKKGEALYPN
                     YLLANQAAARRQVKIRRLLILTQAQMQDSMTVSDAVNVMDDQQRAGVKVFYAFREALV
                     QSPTFQRLEEDFRKHGAAEDMNTAMFDREILIFSQTYGTVPLGMVGTPTPITMINRLQ
                     ISWKPEMIRDLDPAPLFDMTRYVFEYEGVGSFREQLARFTRSMRELPRRAV"
     gene            20806..>22244
                     /locus_tag="PKK81_03760"
     CDS             20806..>22244
                     /locus_tag="PKK81_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNV32015.1"
                     /translation="MAGVKKSVGQEAGMTTSAGAGGAVSGGSSHPRKHWQLLRRWRRT
                     FASVTALLLLGVLALPAYSQTGSQLGPLPPKLPQLPVVPPPAQPPAIEVPAPRPPSAL
                     DQAAKGPKIMVKDIRLVGNTAFTSQRLSEITTPYTNRELTAEDLESLRLALTSYYVNH
                     GYLTSGAVVPEQDVADGTLTMQIIEGKITQVNVEDTKWFRPSYFQSRVNLAAGPPLHV
                     EALQERLRVIQANPRIERINAELLPGAAIAESTLNMKVKEANPLKAWLEFNNYQSPVV
                     GAEQGFVTLAHQNLLGFGDTLSLQYGRSAGVNPMLNFKYEIPVGPRDTTVALQYRRFD
                     FGVEEAPFDALDIKNKAQIFGLSVRHPVIRTADQELAFSLTGEHARNESTLGGNPFEL
                     ITGSPNGKFRVTSLRFGQEYARRSADQVISLLSRFSVGVGAMGATANGDPNLPDARFF
                     SWLGEAQWIRQLPLWRTQLVSRGVVQLSN"
BASE COUNT         4295 a         6336 c         6925 g         4688 t
ORIGIN      
        1 ttggcaatct cttcgaagaa gtcattgtgc acgttgaaga agaaagagag ttgcggcgcg
       61 aaggcatcca cgtcgaggcc ggacttcacg gccgcatcca cataggtgag tccgtcgtac
      121 agtgtgaacg cgagttcttg aacggctgtc gagcctgcct cccggatgtg gtacccgctg
      181 atgctgatcg gatgccactt ggggacatga gcggcacagt aggcgattgt atcgacgatg
      241 atcgcgatgt gcggttcggg cgggaagagc cactcctttt gcgcgatata ttctttcaga
      301 atatcgtttt gaatcgtgcc gccgagtcgg tcgaacggga tgccgcgttt ttctgccacg
      361 gccagataca tggcaaaaag taccgcagcc ggtccgttga tcgtcatcga ggtggtgacc
      421 tggtcgagcg ggataccgtc gaacagccgt tccatgtcgg tgagtgagga gatggccacg
      481 ccgcagtgac ctacttcgcc ccgtgccatc ggatcatcgg catcaaggcc catgagcgtc
      541 ggcaggtcga aggcgacact gaggccggtc tggccctgtg ccaggagata cttgaagcgc
      601 cgattcgtat cgtccgcgga gccgaaaccc gcgaattgcc gcatggtcca gaggcggctg
      661 cggtacatgc tcgggtagat gccgcgggtg taggggaatg agcccggttg gccgagatcc
      721 cgttcgacgt tccaatcctt cagatggtca gagctgtaga cccgttcgat gtctaatcca
      781 gagagggagg agaagcgaga tttccgctcg ttcatgatgc ggccctccgg gaaggaacga
      841 gaacgtcagc tcacagtcac cggctggtct ccatacacat agaatccatg tccgctcttt
      901 ctccccagcc gaccggcttc gacatacttg cgtagcaggg gacaggggcg aaacttcggg
      961 tcccccaggt cctgatggag cacttcgcaa attgcgagca ccgtatccag gccgatgcgg
     1021 tcggccaagg ccaggggccc cacgggatgg ttggtccctt ccaccatcgc cagatcaatc
     1081 gcttcggccg ttgccactcc ctcctccagc gcaaaaatgg cctcgttaat catgggaatg
     1141 agaacccggt tgacgatgaa gccgggggaa tccttgcaga ccaccgcggt tttccctgct
     1201 cgacgaacga gttccagggc cagttgcacc gtatgctcag aggtctcgat ggcgcgcacc
     1261 acttcgacca gtcgcatgac cgggacggga ttcataaaat ggatcccgac gacgcgatcg
     1321 ggacgacccg aggccatgcc caggctcgcg atcgaaatcg aggacgtatt gctggccagc
     1381 agggtcgatg gttcgcagac ctgccctaat tgcgcgaaca gcgctcgttt catgacggga
     1441 tcttcgggta tggcttcgat caccacttga gcctctcgta atcgttgcat gtgcttcatc
     1501 ggatgtatga tcgccatggc cgatccggcc tgatctccac gcagggcgcc tttctcgacc
     1561 gcttttgtga ccccctgcca gattttcttg atggcgtctt ccagcgcctc ttccgttgca
     1621 tctatgagga ggacattcca tcctgccgtg gccagcacct gcgcgatgcc gcgccccatc
     1681 tgccccgcgc ctacgacccc gatggttttg atgtcgtcga tcgtcataac gatccctggt
     1741 tcaccgctcc acgatgaggg cgagcgcttc tccgcctccg atgcagaggg cggccagccc
     1801 tcgatgggca tcgcgcgcct ccatggcata cagcagcgtc gttacgatcc tggctccggt
     1861 gacgccgatg ggatgaccca gggcgaccgc gccgccgttg acgttgacct tcttgggatc
     1921 gaggccgagt tcacgattga tcgccaggga gacggccgaa aacgcttcat tgatctcgaa
     1981 cagatcgatg tcaccgatgg aaagtccggt ctgtttcaac agccgctgga tcgcttcgac
     2041 cggcgcaatg gtgaaccatt ccggcgccag cgcggcaccg gcatacccga cgatgcgagc
     2101 catcggcgtg agaccaagcc gagcggcttc ttcttcaccc atgatgacca atgctgccgc
     2161 tccatcgttg caggaggggg agttgcccac ggtgaggact ccgtcgtcct gaaacaccgg
     2221 tttgagctgg cgcaacttgg tcagatccac ccgattcggt tcctcgtcgt ccgagacgag
     2281 gaccgaagct ccctttttct gcggtacttc gaccggaacg atctcttttt tgaaggcgcc
     2341 gcgggcgatc gcgtcacggg cccggctgta actttccagg gcgaagtcgt cgacgtcttg
     2401 tctcgacaat cgatacttgg ccgcgcacaa ctcccccgca ttccccatgt gaaactggtt
     2461 gtagacatcc cagagcccgt ctttgattaa actgtcggtc agctcaccgt gccccagtcg
     2521 gtagccttgt ctggctttct ccagcaggaa cggggctcgt gtcatgtttt ccattccgcc
     2581 ggccaccatg acgttcgctt cgcctagcgc gatggccctt gtggccatca tgacggcctg
     2641 gagactggat ccgcagacct tattgaccgt caccgcaccg acggaatggg gcagccccgc
     2701 gccgatggcc gcttgtctgg ctggtgcctg acccaatcct gcgctgagca cgcatcccat
     2761 caggacttcg tcgatccgat cgcccggcag ttggagacgt ttcacggctt cggcgatggc
     2821 gaggcttccg agcttggttg ccgggactga gctgtagctt ccgttgaagc tgcccattgg
     2881 agttcggact gcgctcacga tgacggcatg gtgtctggcg ttcacaacag tttctcccag
     2941 ccgctctctt ctagatgctg tttcaccctg gccatgaagc ggtcggcggt ggcgccatcg
     3001 atcacccgat ggtcgaacga gaggctcagg taacacatgg ggcgaatcgc gatggcatcg
     3061 ttgatgacga cagcacgctt ctgcaccgac cctactccga ggatggcagt ctgcggttga
     3121 ttgatgatgg gggtgctgaa gaggctgccg aagccgccgt ggttggtgat ggtgaaggtg
     3181 ccgtcctgga cttcttcggg tcttaaccgc ttgctcctgg ctcgttcggc aaggtctgtt
     3241 atctcatggg caatctggtt cagtcctttg cgatcggcat gccgaatgac gggaaccagc
     3301 aggccgtcgt cgagtgccac tgcgatgccg atatgcatgt cgttcttcat cacgagtccc
     3361 tgctcccgcc aggaggaatt cagaagtggt tgctctctga tagcgcgcgt gacggcgctg
     3421 atcacgaacg gcagatacgt gagggcgcgg ccctcgcgga atccggcaat gccggtgaag
     3481 tccgcttcga aaaaggtcgc cacatgggcc gccgtgtgcc ggctctggac catgcgctcg
     3541 gcgatggtct ttcgcatttg agacaggggc acgatctctt caagcgtgga ggcgggaggc
     3601 ggggatggcg ttgtgcctaa cgccccctgc acgctgccgg tcgactcacg cttgctgatc
     3661 acgttcaaca ggtcatgctt cgtgactctc ccgccctctc cgcttcctgt gattgcggag
     3721 agatccaggc catgttcttt cgccagttgt cgaacggcgg gtgagtagtg aggttcgccc
     3781 tgcgctgcct ccatcggccg gaccgtcaca cccccgacac gattgacgac gccggaggcc
     3841 ggctgagtat cgagcctggc cagcattgtc cctaccggaa cggttgttcc ttcaggcacg
     3901 agtacctcgg tgaggaaccc tgtcgcgggt gagggaatgt ccagagcgac cttctcggtt
     3961 tccacttcca ggagcggctg atctttttcg acggctccgc cttgagggat cagccatttg
     4021 acgacggtcc cttccgcgat gctttcaccg agttggggca tcacaatgtc ggtcgccact
     4081 tcaacctccg gcgatcaact ctcagcggtt aaccgccagc ttcagagacc ttagaaatca
     4141 ccttcctgaa ggccgattgc tgacggctcc tttccctctc aactcaatag gccgccaact
     4201 tcttcgctcc cgcgacgatg tcgctcactt taggcagaaa gaattcttcc agcggcgtgc
     4261 tgaacggtac cggcgtgtcc ggcggggcga tgcggagaat cggcccatcc agagagtcga
     4321 agcagtcttc cgccagcaat gcggaaattt cggccccgat cccgccggtt ttgttgtcct
     4381 cgtgcagaag gacgaccttg ctggtcttgc gcaccgatgc gtaaatcgtc tccttgtcga
     4441 gggggatcaa ggtgcgaagg tccactactt caagatcgat tccttctttg cctaacagtt
     4501 ccgccgcttc cagggccaga tggaccatcg ctccgtacgt gatgagcgag acgtcgcggc
     4561 ctgttcgttt cacctcggct tttccgaggg gaacgatgta atcctcctga ggcaaggcag
     4621 ctttgatccg gcgatagagg aacttgtgtt caaagtagag aaccgggttg ggatcgcgaa
     4681 ttgccgcctt caacagtccc tttgcgtcat agggtgttga aggagcgacg atcttgagtc
     4741 cgggagaatg gaagaaccag ccctcgggac attcggagtg aaagggaccg ccgtgaacgc
     4801 ctccgccgaa cggtgcccgg atgaccaacg gaaccgccgc gccccagcga taatgattct
     4861 tggccgccac ttcggtgatc tggtcgaacg cgcaggaaat gaagtccgca aactgcatct
     4921 cgaccaccgg tcgcaatccc atcatggccg caccgatggc cgcgcccaca atgccggatt
     4981 cggaaatcgg cgtgtccagc acgcgccatt cgccatattt tttgaggaac ccttccgtga
     5041 ccttaaacgc cccgccgtag gcgccgatgt cttcgcccat cagaaagacg cgctcgtccc
     5101 ggctcatctc ttcatccagg gcctgcgaaa tcgcgtctac gtaggtaacc tcctgagtga
     5161 gcgtcgcggt cgtcatgggt gcacctcgtc ttctatggcg aacactccca ccagcgtttc
     5221 ccgcccttcc ggcagggggc tctgttcggc gaattccgtc cctgcgtcga cttctttctt
     5281 ggcccgttct cccacctcat gaaaatacga ttcatcgcca taccctaact gcgtcaccag
     5341 acgttcggct ctcaagatgg gatctttggt tttccattcc tccagcaatt cgcgcgggac
     5401 gtacttggcc gcgtcatgtt cggaatggcc atgcatccgc atggttttaa attcgagaaa
     5461 ggtcgggccg tcgccggccc gggccttggc gatggctcgc tgggatgcga ggtagacggc
     5521 cgccacatca ttgccgtcca cgatctcgcc cggcatcccg taggctttcg cccgctcgac
     5581 cacatcggta atcgccattt gataccgcac cggcgtggaa taggcatatt gattgttggt
     5641 gcagaagaac acgaccggca gccgccgtac cgccgcaaag ttcatggctt catgaaagtc
     5701 gcctcgactc gttcctccgt cgccggttcc ggcaaaggcc acgcgggact ctcctctgat
     5761 cttgaaggcc agcgccgcgc ctgcggcgac cggcatgttg tcggccagat ggctgacgaa
     5821 tccgatcatc ccgcgtttca aatcgcccat atgaacattg ccgtcttttc ctttggtagg
     5881 gccaccctgc ttgccgaggt actgggcgat gatttctccg ggggaaaagc cgcgaatgag
     5941 aaacgcgccc atatcgcggt gaaacggcgc aatgacgtca tccggcctca gcgccgaggc
     6001 ataaccgacg gcgatcgcct ccatcccatg actcgtatag acgccgccga cgatgcggcc
     6061 ctgccggtag agcgcgctga tccggtcttc caggcttctc gtcaaccgga ggtagtagta
     6121 catatcgagg aggtcgccgc gtttgatttc attggcaatg accgcaacgt ccatgatgac
     6181 ctcccgagtg aggtggccag gtactcagag tgccggaaga tcccgccatg acaacaaggg
     6241 tttgtggtcc ttgcacgagt gacaatcgga cgcgtcccac tgcggcatgt ccaggtcaac
     6301 gctgaagtaa aagggaaaac gcgccgtgag ttcgtccatc aaggggaatt gcccgctgtc
     6361 tcgtctggca aaaaccatca tgcctgcaag ttgtcctccg cgatccgtca cgacctttcc
     6421 caatttgctg acgcacagtc cgcgagtggt cacgtcgttc aaggagagaa agcgttctcc
     6481 cgtttcgact gtgccttgga tgatgtcggt gccgatcttg cccgtctcgt gattgaacgg
     6541 ggtcaggacg acgcggagcg gcatctgtgg agccaggatg tcgctggttc gctcggccag
     6601 aaatcgcgcg tcggatgagg tggtgagaat tcctgtcggc ggatggtgtc gaaacgtctg
     6661 cagaatccat tccgccatgt tggatgcgat gatggtcacg agatcgggga atcgcgcgat
     6721 ggattcaaag cgcagataga tatccgtgtg gtggccggag accacttcca catggctatc
     6781 gaaatacacc gctcgcgagt catgcagcag gcgctgaata tcccatgacg acagagtggg
     6841 atgcggggtg tcgcgaatca ggtgttccag cctcacgtcg atctcagaac cctcatagag
     6901 cttcctctgt cgttccacca actccagggt ggcggtttcc ggggatttgt cgattgacgg
     6961 catcatgacc cgtactcctc tagttctgtg cggcaatggt cgttctcaga atgccgatcg
     7021 gcagaatctc caattcgacg acatcgcctg gtttcaaata tcgatcaagc tccaggccgc
     7081 acccgccgcc gactgttcct gagccgaaca gatcgccggg atataggaac tcacctcgcg
     7141 acacgtgctc gatcatttgc gaaaacgacc aatggattga accgaaacgc ccctcagacc
     7201 agacctcgcc gttgatacgg gcgatcatcc gtaacgacgc gaggtcctga atgtcatcgg
     7261 gcgtgacgat gcagggaccg atggcggtgg cgaaatcttt tccttttgcc gggccgagcc
     7321 ggcaggccat ttcctgaaat tgaatgtctc gcgcgctgaa gtcgttcatg atcgtataac
     7381 cggcgatgta gttcacggca tcccgctcgg caatatcgat tcccttgcgt ccgatgacgc
     7441 aggccagctc cagttcgtaa tccagtttct gtgtgttcag cggccaccga agctcatgat
     7501 caggcccgat gatcgtccgg tggttgcctt tgtagtacac gggggccttg taccattcag
     7561 gcgggatcgg ttgtccccgt cgcttggagg tcgccgcaat gtgctcctcg aaggcaataa
     7621 aatcacgcag ggatgaagga ttggggaggg gagccgtcaa tcggacgctc gccggatcat
     7681 aaaagatggt ctcacccgcc ggacctgcga tggaggaacc atggcctacc gcatagtcga
     7741 atgcgcgacg ggccgcgtct gtcgcagcgg ggccgccttc caaaaactcc aacatggtgg
     7801 cggggacctg cgcgtcggcg aggcggcgcg gttgtgtttc gccctgatcg gtgagtcgtc
     7861 gcgcctgcgc catgttgagg tctacgatcg aggcattgtg caacgcaccg attctggtga
     7921 aggttccgac gggagtgcgc acctggaagc tgacgagttt catgggcggc tccaactcat
     7981 cgcatagtcc ttcagttcaa cggcgtcaca ttcgggcatc accgtaaagg cccgtcgcga
     8041 ttcgatcatc acggcgactt cgtccgtctg ctccttggtc ttgctcgcgg cgatggcctg
     8101 cgggtgcggg ccatgatgaa tcccttgtgg atgcagcgtg aacatgccgg gatgaatgcc
     8161 ggcgcggctg aaaaaatctc cctggtgata aaagagcact tcgtcatagt ccatgttgcg
     8221 atgataaaac ggaacgcgaa gtgcgctcgg gtcgctttcc agcggccttg gcgcgaaggt
     8281 cgatatcagc agtccgccgg cttgaaaggt catgtgcacg ctgggcggga ggtgatagcg
     8341 cgggctgatg accggacgaa aatcccggac attcaacttg gcgacccaga ggtcaccctt
     8401 ccaggccgcg acatccatgg gatagaacgg gtaatacacc cgggtgtagt caccgtcccg
     8461 cttgatgcgc acttcccatt cgtgtcgact atgggcgtct tccggaggca gcgccagttc
     8521 cggcgcggtg aggactcccg gatcaaataa cgcatggcgg ccgagcgggc ctcgatccgg
     8581 cacttcgaca ggcgccgtag tctcgaccat gagaaacagc cccggctctt ccttcgtatc
     8641 gacatggact cgatatgttg tgcctttcgg gatcaccagg tagtcgcccg gttcataact
     8701 caagaatccg tagtccgttt cccaccgacc gcgccctcga tgcacaaaga cgatttcgtc
     8761 cccgtcggca ttgcgcacga agtgaggcat cgtttccgca cgtctggaca gggagacacg
     8821 ggcatccgga ctctgcaaga ccagggttgg gcgtccgtcg gcggaatggt aatcggcggt
     8881 cggcagctcg gtgcaggtaa acgcccgggg gcggagcgga ccgtcgatcc tgctccacgc
     8941 agtcggtgga tggtgatggt agaggtgcga ggaggggccg ctgaacccct gacgaccatg
     9001 ctcttcctcg tggagtccgt cgggaacacc gacgtgtgcc tgattgggag gggcgccttt
     9061 tttcactaag tacatcacga acctcctgat gcctcaggga cggctcgatg ttttacgacg
     9121 tcgactgaaa cgactcggat ggcgtgtctt taaagggaag cggaagggtc tcgtagtcga
     9181 tgatcgtgtt ctcgatccct ctggtttgat ctctctcaat gtctctatag agcgcctcga
     9241 cggtggcgcg cacgaaggtc ttggattcct ccagcccgtg ctgccgttgg gtcagttcca
     9301 ggaacaggcc gcacccgggg aagaggggat aggtgaatcg ctgcttgagg ggcccgaagc
     9361 cgtcgcggcc attcttggtt tctccggaga atcggactcc atgggattcc cattcgcgac
     9421 agaccgcttc gatgtcatcg acctctaggg cgatgtgctg aaccccttcg ccgtatttat
     9481 cgataaagtc acagatctgg gaccgacgtg ccttattgcg gccttgcatg agcgcgatct
     9541 tcgcatcgcc ccgttgaacg accacggtgt ccatgctcga catgtcactg ccgacatcct
     9601 gagcggacca aatcacgtga aagccgagta cggtggtgaa gagggattcg acggcggcga
     9661 gatttttcac gcagagcgtg atgtgatcaa taccgatggc ttctttcatg gtgggtcctc
     9721 ctacgcggcc tcggaaaacg tggaagggac tcatgccatt gtcgcgatcg ggaagagcag
     9781 gattcacata cgcccacact atataacata atagttgcat cattcaatat agattttgta
     9841 ttatttgcca tggcgacgaa catgagaaag gacattcgca tgaagttcgg ttgggacgag
     9901 acgggacatc ccggtatgac cgcggaggtg cacgatgtcg ttttacgaac atgtgcgcgc
     9961 ggacgtgctc cggcatggag cgatcaataa tacctacctg acacggtttc aagccggtga
    10021 ggtgaccgac agagagttcc acgaatttgc cgtcgagttt tatagttttg cgcgattctt
    10081 tccccgcatc cttgccgctc aattagtcaa cactgaagat gaggccgtgg ccgatgagct
    10141 gacgaaagtc ctctactcgg aactcggcga cggcatcgtc aagcatcgcc acgaattgct
    10201 gtaccggaac ttcctccggt cgattggcat cgacattcat gacgcgatgc acacacccat
    10261 gaaacccagc acaagggcgt atatccaggg aatggaacgg ctttatagca gcaggcacca
    10321 cgcgacggcg ttgggagcgt cctttggtct tgagaacatg gccatcacga tgtgggacca
    10381 tttgattcca gggttgaccc ggttgaagca ggtccggtat ccgcgcatgg acatgacgta
    10441 tttcaccttc caccgccaac tcgaatccgc gcatgaaaaa gccatggagc atgccgtgga
    10501 agctgtcgga ggagcggggg cgaatgggtg cgcaacgatg agcgcacaag agcatagaga
    10561 tttttgcctg ggaatgaacg cggtgctgga ctatctcgaa gggttttgga tggggctgga
    10621 acgaaaggcg tccggtatcc gacggagccg gcctcagccg gcgcggcagt tggcgagtga
    10681 actgcgcgga caatcgcatt gacccggctg cgggtcggag gatcacatgg agcgcgtgtg
    10741 gatcagccgc tacgatgcgg gtgttcctct ctcctccgcc tatccggaat ggaccgtccc
    10801 ggacctgctt cgtcgctccg cctcctgttt tcccgaatcg accgctctct tcttttatgg
    10861 cgcgcgtctt tccttccgtg aactgaacga tctgaccacg cgttttgcac atgggttgct
    10921 gcggctcggc gtgaagaggg gcgatcgggt cgctctcatg ctgccgaata ttccacaggc
    10981 ggtcatcgcc tattacggcg tactaaaggc cggagcgatt gtcgcgccca tgaatcctct
    11041 atacgtcgaa cgtgaaattc acagtcagtt ggccgacgcc ggcagcgaga tcatcgttgc
    11101 gttggatctt ttttatcccc gcattcaggc cgtccgcgag cagacgggtc tacccgaacg
    11161 gatcatcatc accagcctcg gagattttct tccgaccgtg aagcggctgc tctatccgct
    11221 taaggcccgg ttggcaaagc gctgggtggc ggtcgagaaa actcccccgg tctatgactt
    11281 tcttgcactg ctcgacgcgg catccccccg caccgaagat gattccccgc ttctacccac
    11341 ggtggagccg gacgcgctgg cccagattca atataccggc ggcacgaccg gcacccccaa
    11401 gggtgtcatg ctctcgcatc gtaacgtggt ggtcaatgcg atgcaggggc gcctctggtg
    11461 ttcggatttt caggagggga aggaagtttt cctgggagcg gtgccgtttt tccattgtta
    11521 tgggctgagt acttgccaga atctcgccgt cgcgactggt tctcagatcg tcctgctccc
    11581 gcgattccat gcggaggagg cggtgaaatt gattcatcag caccgggtca ccattgtgtc
    11641 gggcgtgccg gtaatgttcg ccatgatcac agattgtccg aaagtcggac ggtacgactt
    11701 acattcgata cgggtctgcc tttgcggagc cagccccttg ccgaccgagg tgcaggaacg
    11761 attcgagcgg ttgagcgggg tcaagatttc agaaggatac gggttgaccg aggcgggtcc
    11821 caccacccat tgcaacccga ttcaggggga tcatccgcaa ggctcgatgg ggttgccgtt
    11881 tcccgatacg gacgcacgga ttgtggatga agagacgggc atgcgggaca tccctacggg
    11941 agaagcgggg gaactgatca tccgcgggcc tcaggtgatg cagggctact ggaggaaaac
    12001 cgaggacacg caagcggtgt tgcgcgacgg ctggctctac accggagata tcgtcacgcg
    12061 tgatgagcat ggatttttct tttttctgga tcgcaagaaa gatgtcatca agccatgggg
    12121 ggagaccgtc tatccgcgag aggttgaaga aatcctttat cagcatcccg ccgtgcgtga
    12181 ggcggtggtg gtgggcgctc ccgaccacca ttacggagaa gcggtcaagg cgtttgtcgt
    12241 gcagaaggag ggatgtgccg tcacggagag agagttgatc gagcactgcc gccggtcgct
    12301 ggcacggttc aaggtcccgg tggctatcga atttcgcact gaattgccac gcaccattat
    12361 cgggaaggtg ttgcgacgcg ccttgcgcga tgaggcggat ccggtcccgg ccggtatgca
    12421 gtcgtcgcgc aaggcgatgt gaggctgcga ggagggcacc atgagagagg tcgtcatcgt
    12481 cgccggcgta cgcaccccta tcggtaactt cggcggtgcc ctgaaggacc ttccgcctca
    12541 caagatgggg gaattggtcg tgcgtgaagc cgtctcgcgt gccaaggtgg atccccggct
    12601 catcgacgaa gtgatcgtgg gctccgttgg tcacacgagt gatgcctaca acgtcgctcg
    12661 tgcgattgcg ctcatggccg gactgcctgt gcgtaccccg gcctattcgg tgcagcgaaa
    12721 ctgctcctcc ggcctccagc cgttcgtcaa tgcgtaccag aacattcaaa gtgaggatgc
    12781 agacgcacaa gtcgtggggg gagtggaaag catgagccgc gccccgtttg tctcacggga
    12841 catgcggtgg ggaaaacggc tccgcaacgc cgaattgatc gacagtatct gggagggatt
    12901 gaccgatgcg ttttgtggcc aattgatggg ccggacggcc gagaacctgg ctgaggagtt
    12961 tggcatcggt cgtgaggagc aggatcgata tgcggtggag agccaccgcc gggcgttcaa
    13021 agccattcgg gagggccggc tcaaggatga gattctgcct ctgatggtgc cgaagtcggt
    13081 ggccggacgt gatgtcgcac cggtggtcgt ctcgcaagac gaggggccga atgtcgggtt
    13141 gacggaacag caactcgcgc tctatccgcc gcttttcaaa gaacagggga gcgtcacggc
    13201 ggggaacagt tgccccctca acgatggcgc cgctgcagcg gtcgtcatgt cctccgcgcg
    13261 tgcgcgtgag ttggggtgtc gcccgctcgg ccgcatcaga gggtatgcct tcatcggcgt
    13321 ggagccgacg cgtatgggga tcggtcccgc cgaagcgctc ccgttggcac tcaagcgcgc
    13381 cggtgtcacc ctcaccgatc ttgaactcat cgaagtgaac gaggcctttg ccgtgcagta
    13441 tctcgcggtg gaacgtgtgt tgggactcaa gcgggagctc gtcaatgtga acggcggcgc
    13501 gattgcgctg ggtcatccgg tcggcatgac gggtgcccgg ctggtcatca cgatcctgta
    13561 cgagatgcag cggcgtggcg ctgcactcgg tgcagtggcc ctgtgtgtcg gcggcgggca
    13621 gggcgctgcg atggtgttgg aacgcatgtg accggtgcgt gtcgagcgac aggagtcgtc
    13681 ccatgtacat ctataaagtc ggtgtggtgg gtgccggtac catgggcgcg cagattgctg
    13741 aagtggtgag ttatgccgga gtgccggtgc tgctggcaga catacaggaa tcactggcca
    13801 ggagaggtgt cgaatccgtg cgggcgatat atcaagcccg cgtggacaaa ggcaagatga
    13861 cgcctgagca gctggaagag aaaatgctgc tggtgacggc ctcgccgaac ctcgaggctc
    13921 tccatgatgt cgatctggtg atcgaggcgg tctcagaaga ggtcacgctg aaacagcgcg
    13981 tgttccgcga actggaccgg gtctgtgcgc gaagtgccat cctggcgagt aacacctccg
    14041 cgctctccat ctcggccatc ggggcttcaa ccacgaggcc cggtaaggtg ctcggacttc
    14101 attttttcaa tcctgcctat gcgatgccgc tggtggaggt catccccggc ctggccaccg
    14161 atccccagac cgtcgatgat gtcgtcggtt ttgccgaaag tctccgcaag acgccggtga
    14221 tcgtgaagga atgcgcaggt ttcctcgtca atcgcctgct ctctccctat ctgaatgaag
    14281 ccgtgtggtg cttccaagac ggcgatgtgt ccatcaagga gatcgatcag gacatggtgg
    14341 cattcggcat gccggtcggc ccattcatgc tgctggatac cgtcgggctg gatattgcgt
    14401 tcgaggtcgc gcgcattctc caccgatcct acgggccccg gatggcgccg gcgccgctcc
    14461 tcgaagcctt ggtcaaggcg gggcgatggg gaatcaaatc agggcggggg ttctacgagt
    14521 attcggaggg cgaggaacgg agcggcgatc aggacctcga tactctgctg cagcaagtgc
    14581 ggcagggcgg cgggcagacc aggatggaat ggaccagatt acgccctctc ctcgccatgg
    14641 tgaatgaggc tgtgactgcg taccaggagg gcgtcgcgtc ggcgcgagat atcgatctgg
    14701 cgatggtggc gggaacagga tttcccaacg agaagggagg accgctgcat gttgccgacc
    14761 agcttgggat cgaccatgtg ttgcatgaac tggaagcatt gcgggatacg ctgggcgtac
    14821 gcttctggcc ggcgccgatg ttgcgccgaa tggttgacgc cggcttcacc ggacagctcg
    14881 cgggacgagg gttctttgcc tattaacgct cttgtcggcg gggcaggatt agtatgctcg
    14941 tccctttccc gatcgcatcc atgtgggaat acccaccatt caatgctgtg aggctcctat
    15001 gacgacgaca gccggttcga tgctttcttg cactgtcgag ggggcggtgg ccactttgtt
    15061 gatcaaccgt cctcccgcta acaccctgac acctgaactc cttgccgagc tgagcacgac
    15121 gttcgaccag gttgccaagg acgaagcggt caaagtggtc gtgttgaccg gcacgggtcg
    15181 attcttcatt gccggggcag atattcgagt cttggcctcg atcgcttcgt cgaaggaagg
    15241 ggaggccatg gctctgcagg gccaggccat cttggatcgg atcgaagggg ttggaaaacc
    15301 ggtgatcgct gccatcaacg ggatttgcct ggggggagga ttggaattgg ccctgtgttg
    15361 ccatatcagg cttgccgcgg aagggagccg attgggccag ccggaaatca atctggggat
    15421 catgccggga ttcggcggca cgcagcgact tgcccggatc atcggtcagt cgaaagccat
    15481 ggagctgatc ctgaccggtg agccgatctc ggcgcaggaa gcaaagacga ttgggctcgt
    15541 gtcgcaggtc gtttcccctg aggatctcct ccgtcaggct caggggttgg cgcgcacgat
    15601 tgcctcgaaa ggccagatgg cggtgcgcgc gtcgcttcgg gcgatccgcc agggtgtcga
    15661 gctgaatctt catgacgccc tggccttgga agcgcgcctg ttcggagagt tgtgcgacac
    15721 ggcggacaga cgagaaggcc tgtcggcgtt tctggagaag cggcagccgc atttcatgga
    15781 tcgatgaatg gccgttgtgc cgttgagtcg atgtggagcc cacgatggca ccgactttca
    15841 gcaagcgggc cctggcgttg ggcggaggtg gcttcaccgg ctacctcttc gaaatcggcg
    15901 cgttgaccgc cttggacgat ctcttcgagg acggtgtgac gatgaacgat ctcgatctgt
    15961 atgtcggcgt gagtgccgga gcggcggcgg cgtcactgtt ggccaatgga gtcaggccgc
    16021 aggagattct ggacacgaat ctgtccggca ccagacccta ctacttcgat catcgtaacg
    16081 tgttttctcc tgccatcggc gaggggctga aaacggtgtg gcgggtgacc cggcaactcg
    16141 ttccgctgct gaagctctat gtgcgtcact atggagagat gacgttcatc gatttgctcg
    16201 ataaggcgca ggatgcgctg ccgagcggta tttacacgct ggagcctttt gcgaagtatc
    16261 tcgaggcgat cttcaaagtc aaagggttga gcgaccggtt cgccggactc gccaaggaac
    16321 tgtacatccc ggccatcgat ttggaaaccg gcgatggcgt tctgttcggc gacgaggatt
    16381 ggcgggacgt tccgatctca cgtgccgtga cggcctcctc cgcagtgccg atttacttct
    16441 gcccggtccg gatccagggc cgggactaca tcgatgccgg catcggtcgc atggccttct
    16501 tcgagatcgc cgttcaaaaa cacgtggact tcatgatcat gatcaatccg atggcacgcg
    16561 ctccgcagac gcggtcacgg caagcgcagc cggccgtcgg caagcccgga aatcgattgc
    16621 gagacagggg ctttttgtcc atcggggaac aggcctcccg catcaatttc gacgcgcgtt
    16681 tctcgcaagc cttggagcgc ttccagcagg actatccgga gaaggaagtg ctcgccattt
    16741 cgccggtgga agccgatgcg ctgctgttcg aacggagttt tctgagttac gacgaccgga
    16801 tccatctcct tcgggccgga tatgtggcgg tgatgacgct ggcacgggat cggtgggagg
    16861 acctgtccgc gcagttcgcg cgccatggtc tggtgatgtc gcgggccagg ttcgaggacc
    16921 gcgccgggcg gcgcctcgcg cggctcgatg aagtcgcgag gtttgctgat ccgaacaccc
    16981 ttcagcccat ggtcggaccg gcacgcatcg gcggtatcgg cgcggcctgg cgcaaggcca
    17041 agtaagcaac gccgcagagg tacgtatgac gaacggagca gagatgttca aggggtcgct
    17101 cgcggcagcg gaagccgctc gcgacacgcg tgcctattca ggattgattg cagggctatt
    17161 cgaaggagtc gttcggagat cgctgtttac ctctgcatcg attccggcga tgagcgggga
    17221 tgcgaaggaa tttctcgaca ccctccgcct gctgctgatc gagaaggtag atccggaagc
    17281 catcgataga gaaggcgcga tcggcgaaga ggtgatcgag gcgttcagag cgatcggcgc
    17341 cttcgggatc aaaatcccgc gcagctacgg cgggttgggg ctttcgcagt cggagtatca
    17401 cacagtggcg accctcctcg gcagtcatga cgcggcgacc agcgtgttgc tgtcggccca
    17461 caattcgatc ggagtggcgg aaccggtcaa gctggtggga aatcgtgagc aacagcagcg
    17521 gttgctgcct cgattggcac ggggagacat atccggattt gcgctgacgg aaaagagtgc
    17581 ggggtgtgat atctgggatc tcaaaaccta cgcgattcct gttcatgaag gcggcgtcct
    17641 ggtgggctat cggctcacgg gtgagaagtt gtacacgacg aacgcgcctc gcgaggatca
    17701 tgagttcctg gcctcactcc ttgtcgtcat tgctcagatc gtcacggtgc ctcaggacgt
    17761 cgaccgtccc aaggaagagc ggcgattcgg ggcgtttgtc gtggagacgc agtctgcggg
    17821 gtgccgctgc acgcgtctca gctttatggg tgttcgaggc atctacaatg gccaggtgca
    17881 tctgcgcgat gtttttgtgc cggtagccga ccggctcgga gaggagggcg aaggcctgcg
    17941 gcgcgctttg gagagtctca cggtcggacg attgacactg cctgccgcct gcctgggcag
    18001 tctgaaacaa tgtctctggt tagctcgggc tcgcgcgcag caacgcattc aatacgaccg
    18061 cccgatcggc gagcatacgg atatcggttc caaaattgtt ctgatggcgt cccgcgtgct
    18121 cgcgctcgag gccatcgtga agatcacggg aatctggacc gatgccaagg tggatgtacg
    18181 gctggagtcg gcggcggcga aagtcctcgc gactgagtgg ctgctggagt cgttgctgga
    18241 tctgttccgc atctacggcg ggcgcgcgtt tgagacgccc gattcgttgc gtctccacgg
    18301 ggacttgccg gtgccgatcg aacggatgat ccgggacgcg ctcatcaatg tgatctggga
    18361 agggagcaac ggtattctga cgttgtggat tgcgcgggaa ggactcggcg aatatgtgac
    18421 gcaaggcagg gcttttctgg aacgcgggat cgaggacatg gctcaggctg tgccattttt
    18481 cgcgagaatc tccgcgcgag ccttcaatcc gattgccttt cttgggcgag gtgtgcgatc
    18541 gggggctgat gcggctccgt gggagcggtt tgtgtttgaa gagagtcgtg cgctcgcgcg
    18601 gaaatccctg tgggccagtg tccgtcaccg acaggggctc gcgcgcaatc aacgcttgtt
    18661 gagacgtctg gtgacagccg gcctgcagct atttgccgtg gaagccttgt tgtggtacgg
    18721 atcacagccg gaagtccggg agcatgcctt gtcgcgggcg ctggttgaag atttctgtct
    18781 gcgagtcaaa gaaacatttc atcccacccc gttgctctct ctgacgacgc ccttgtggga
    18841 tgacgatcgg aggttgtttc atctggccaa agacatactt gcgggcaagg ccggatggtt
    18901 ggaggagggc atcatcccgt cttgcccggg cgggcgaacc ggtgttgccg aaccggccgg
    18961 tgtggtgacg ggcgcttcgg tggagacctg gtcagggatt tgaggcggag atgagagtgg
    19021 ttgttgcttc ccaggattca tgttcaacgg agtctctatg acggaaattt ccgggtactt
    19081 cgaggcgatt aaagaccgct acgcgttgac cagtgattat acgctggccg aaaagcttgg
    19141 cattgcacaa ccggatgcca acttgatgcg acgcggtctc aaggttccca aaccggagct
    19201 ctgcatcaaa atcgcgaagt tgctcgacag gaatccggtg gaactcttgc tgatcgcgca
    19261 aaaggacaag gccccgaaac aggcgaaaga atactggacc ctggcgctga cggccgtgga
    19321 tgtcatgctg catgtgccga aacgtccccg gtatctcccg aagaaggtcg aggcaatcgg
    19381 cagggaattg aaacagctgg agtcacaaac cctgacctat gaaggcgccg ccgccaatgc
    19441 cgaggcggtc cgtctgatgg agacggcgga gcagtccgtc gatgcgatga tggaacggtg
    19501 gaacatttgg aagaaggggg aagccctgta tcccaactat ctgttggcga atcaggctgc
    19561 cgcgcggcgc caggtcaaaa tcagacgcct cctgatcctc actcaggcgc agatgcagga
    19621 cagcatgacg gtgtccgatg cggtgaacgt catggacgat cagcaacgtg ccggcgtcaa
    19681 ggtattctat gcgtttcggg aagcgctggt gcagtcaccc acctttcagc ggctcgaaga
    19741 ggatttcagg aaacacggcg ctgccgaaga catgaatacg gcgatgttcg atcgggaaat
    19801 tttgatcttc tcgcaaacct acggcacggt cccgctcggc atggtcggca cgcccacgcc
    19861 catcaccatg atcaatcggc ttcagatttc atggaaaccg gagatgatcc gggacctgga
    19921 tccggccccg ctgttcgata tgacccgcta tgtgttcgag tatgaggggg tcggttcatt
    19981 ccgggaacag ctggcccggt tcacgcggtc gatgcgggag cttccccgcc gagccgtcta
    20041 gcaggctgcg gaacaactcg attgtcgcgc aatacggtct gatcaactcg tgcgccgtgt
    20101 cggtatcagc atcacccgca ggatgcgcaa aaaggccgtc tcctcgtccg ttgttcgtga
    20161 aacgtcgttc gtctctcggc gtcagaaaag agcgtatggc atagagcgca tagcctatgg
    20221 ctagaaatcg ggagtgggcg tccgtactct cagctatacg ccaccagcca tacgctctga
    20281 ccctgacgag atacgcttca cgattcacgc ttcacgggtg tcgcgaacgc cgctggcgga
    20341 ctttttccgc atcctgctag agtcacgccc gccgcatcca tccgatatca ccccacatga
    20401 ccgacggccg tatcgtgtcg caggccgagc catgcgctca ctcatagtgg gctgcgggcg
    20461 ttcagacctg gaacgcaagg tctctcccgc cggccttctc tccgccgccc cctcacgaat
    20521 ctcccttatg aggatcgact tcacccgcac gaaaaaactt ttcgaggcag gagtatttta
    20581 attcgagaca tgttataagc agttccttcg caggtaagac tctccaaaga gatacgtatc
    20641 gcgcggctgt ctcattgtcg cacaggcacg gttctcccga ggagaggcac atgggcgcaa
    20701 caagagacat cgcgcggatg caagggcaat gagggtggtc tggattgttg cgggcagggg
    20761 gacagccacg ctgtgccccg tcatcaagaa agaggggagg ccttgatggc tggcgtgaag
    20821 aagtcggtgg ggcaggaagc aggaatgacg acgtcggctg gtgcgggtgg ggctgtgtcg
    20881 ggtggctcat cccatcctcg gaagcactgg caattgctac ggagatggcg tcgcacattt
    20941 gcgagtgtga cggctcttct gttgcttggg gtgctggcat tgccggccta ttcgcagacc
    21001 ggctcgcaac tcggtccgtt gcccccgaaa ttgccccaat tgcctgtcgt cccgcctccc
    21061 gcgcagcctc ccgctattga ggtgccggca ccccgccccc caagtgcact cgatcaggcg
    21121 gccaaggggc ccaagatcat ggtcaaggat atccggctgg tcggcaatac cgcctttacg
    21181 tcgcaacggc tttctgaaat caccactccc tacacgaatc gagagttgac ggcggaagat
    21241 ttagaatcgt tgcgcctcgc cctcacctct tattacgtca accacggcta tttgacctcc
    21301 ggtgcggtgg tgccagagca ggatgtcgcg gacggcaccc tgaccatgca gatcatcgaa
    21361 ggaaaaatta cgcaggtcaa cgtcgaggat accaagtggt tccggccctc ctattttcag
    21421 agccgcgtca atttggcggc cggccctccg ctgcatgtgg aagcgctcca ggaacggttg
    21481 cgcgtgatcc aggccaaccc ccgcatcgaa cggatcaatg ccgaattgtt gccgggcgcc
    21541 gccattgcag aaagtacgct gaatatgaaa gtgaaggaag ccaacccgct caaggcctgg
    21601 ttggaattca acaactatca gtctcccgtg gtcggcgcgg agcaaggctt cgtgacgttg
    21661 gcgcatcaga acctgctggg cttcggcgat acgctgagtt tgcaatacgg gcggtcggcc
    21721 ggggtgaacc cgatgctcaa ctttaaatac gaaattccgg taggcccgcg tgacaccacc
    21781 gtcgcgttgc aatatcgccg gttcgatttc ggggttgagg aagcgccgtt cgatgcgttg
    21841 gatatcaaga ataaggctca gattttcggg ctgtcggtca gacatcccgt catccggacg
    21901 gccgaccagg agctggcctt ttccctcacg ggcgaacatg cccggaatga aagtacgttg
    21961 ggcgggaatc cgttcgagct catcaccggg tcgccgaacg ggaagttccg cgtgacctcg
    22021 ctccgcttcg gtcaggaata tgcccggcgt tctgccgatc aggtgatctc cctcttgtca
    22081 cgtttctccg tcggtgtcgg cgcgatggga gcgaccgcca atggtgatcc gaacctgccg
    22141 gatgcgcgtt tcttctcctg gttgggcgaa gcccagtgga tccgccagct ccccttgtgg
    22201 cggacgcaat tggtgagtcg aggcgtggtg caattgtcca acga
//
LOCUS       DAOQRM010000024        21766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1616_length_21766_cov_14.195247, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000024 DAOQRM010000000
VERSION     DAOQRM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21766
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1616_length_21766_cov_14.195247"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..1064
                     /locus_tag="PKK81_03765"
     CDS             312..1064
                     /locus_tag="PKK81_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNV32016.1"
                     /translation="MLDLVGLGILAGVVPVYLGIAAALVLRRALPRTWEAGLIGLSTG
                     VLTYLFFDLMHEAVELMGVRDPFSWLLFLGSLFLSFVGLVHLEKHQFLKGRHADQALT
                     LPFVIAAGMGLHNLGEGLAIGTSYAGGEWALSGLLVAGFALHNGTEGFGIVGAAGQSP
                     IPPRTVVLLGLTGGAPTCIGTVLSGLTVSPYLSLVWYALAAGSLLYVVFALVAMTYTS
                     SRKVHMAYGVWTGIALMAMTAMFLTLVGGHRS"
     gene            1284..1787
                     /locus_tag="PKK81_03770"
     CDS             1284..1787
                     /locus_tag="PKK81_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNV32017.1"
                     /translation="MHQDRRARTIDTSIIGIVSSGSPPLSIEASRQRPLHSLRTKPQP
                     FWPTPQITPLNSVIPAARLSALLSKRSGDRLTLKELSTLLGYSEKYTSQIFRKYMGLS
                     FSEYLKQLHLAKATKLLSDQSLTIAQIAESVGFSDAFAFSHFFKRAIGCSPSEFRKQQ
                     TPEAGLR"
     gene            1784..2707
                     /locus_tag="PKK81_03775"
     CDS             1784..2707
                     /locus_tag="PKK81_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNV32018.1"
                     /translation="MNDNRESLARMGGWLVSLTLHGLSLGTALVLAAEFSILPEPRPF
                     RWEVTMVSAPALQPVAADVPTPFSASSVSPSVMPRDKSPRQVRSRPNAANTEAARPLE
                     THSGIIAPAQDGFVRPLATENPSLVVDSVSPGPHTPSADLTSDELVHEAPQVTRVDLP
                     VTTTEPSEIPPPSVDAAEVSHTTSMPSIRESIRLVNRPTPRAREAIVSRILHADYGWL
                     AEMLFAKVEQSKRYPTFAKSRRWQGNVMLQAVIHEDGSISDITIVTPSGHSILDLDAV
                     ALLEQASPVQLKYPLGQSHVVVHIPIGYRLE"
     gene            2716..3243
                     /locus_tag="PKK81_03780"
     CDS             2716..3243
                     /locus_tag="PKK81_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV32019.1"
                     /translation="MQNEHTLPCLTAFEEHHNDLLRFFTHKLGCRDLAADCTQDTYMH
                     LVRMRPSIDVQNPRAFLFRVAANLAVDNLRKLRTRRGALSIEPPPEETASPAPSAEDT
                     LDAKQRVAQLEVAMRELSPKCRRALLLNRLEGKTHREIADSLKVSESMVAKYIVQALR
                     HCRARLHPEESTRSS"
     gene            3336..4325
                     /locus_tag="PKK81_03785"
     CDS             3336..4325
                     /locus_tag="PKK81_03785"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV32020.1"
                     /translation="MPDSTPPDASSPLIDEALAWLVRIHSGHASDEERLACTAWRTQS
                     SAHQLAYAEAEILWRDIGLLPNGDCAGSADLTASMPHGTLPRARRTIKWGLAACVLLA
                     AGWLSWGIVQERLDLASADYRTAVGEQRHVKLEDGSRIYLNTDTAVAVAFTPQQRTIR
                     LLTGEASFTVTPETVRPFTVQSGDVSTRALGTSFLVQRHQDAVTVTVTEHAVQVSATD
                     GRQVPAAVVRQSEQISYSAEAGLGRAHAIDSNAIAAWQRGKMIFEAQPLAAVVEELNR
                     YRAGRIMILNPALRSLNVTGVFDIGDPDAALRMIEQTLRIHDTTLTPYLVLLH"
     gene            4537..7113
                     /locus_tag="PKK81_03790"
     CDS             4537..7113
                     /locus_tag="PKK81_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV32021.1"
                     /translation="MMHLSWQVVVTLSVSAVLVHTAGNLAEARSERLTTSNNSPLLLA
                     QATAAAETSDTVDFNIPPQALGSAITAFGDQANYRLLLPSDLAEGRSTSGVSGRHTPA
                     EALALMLAGTGLSYRMTEARTMTLEPVAATPLPLAPLSQEPAPPNPNTQIQAVPKPVK
                     VPEIVVKEVEDRGYAVDESSSATRIPAPIHDTPRSVEVVTRQVLDDQKVIRFSEALRN
                     VSGVSQSSTQGGQGGTFMIRGFASDLNVFKNGFRDDSTFSARAQRDIINIESVEVVKG
                     PPSYLYGRSDPGGVINQVTKAPLRNPYYSAEMIVGSYGLYRPTIDIGGPLNESKTLTY
                     RFNGMYESAESYRDGVKTERIFLAPTFGWEMSSRTTLRFEAEYMYDRAPIDRGLVAIG
                     NGVAPIPVSRFLGDPSRKLETHHGKATITLWHDISNMFRWRTAFRTAVTSSRYSSLES
                     NFLVGAESDGILNLARYEIPTTVQSHYLQNELHGNFTTGSIKHKTIIGIELGRENSSA
                     TASGDFGGDTSTPGAFSYINIFNTNDRLFLNPALTKFSDASTQNNILGAYFGDQVDLL
                     DNLHVHFGGRFDLFDQTITNHPDDFTATSSQNNKTDSAFSPSVGVAYQPWKPITLFAN
                     YTESFAPQSAGSRSINGNLFNPERGKSYEGGIKYQAFGGRLRSTVALFDTRKKNVLTA
                     DPLNGFFFSVATGEQRSKGVEFDIAGQILPGWDIIANYAYIDARVTKDLLFAEGSRAP
                     NSALHQGSLWTTYFFQEGVVQGFGAGIGMYAQGKRNGIFQCQDPANCQAPFEMAGYVR
                     MDAALYYRKQEVFNKTNLLAAINFTNVLDHRYFSGAQNFREIVYTGAPFTAIGSLKFE
                     FH"
     gene            7807..8916
                     /locus_tag="PKK81_03795"
     CDS             7807..8916
                     /locus_tag="PKK81_03795"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNV32022.1"
                     /translation="MWLNLHLYVGLLGGALFVLTSLTGSLLVFYKTIDEWMNPEQLIR
                     TAGSDQSLSDIVAGARTVHPDWSPPDTLIFPLHERDTFHAWFKDPAATPPEERWHVVA
                     VDPSTARPLSDRQWGSFFVSFIYELHQELLLGKPGEVFVGIMAVFLLVSIGTGLYLWW
                     PALGKLRRALSFQSGGSPIRRHYDVHKLTGLAGTLLLTLLAVTGFYLEFPDAVTSVVR
                     WFSPVRDTSPELQPQSEPQTGLPKLPPDQAIAIARTVFPDAKPMWLGLPQHDRDSYSV
                     GLRQPGEVRQAGGQTEVWIDQYSGAVRRGQDWRQFTRGETLLSWLFPLHNGEAFGLTG
                     RWIIFFAGLTPLLLYLTALRMWWLKRVAHQRRREA"
     gene            complement(8952..9578)
                     /locus_tag="PKK81_03800"
     CDS             complement(8952..9578)
                     /locus_tag="PKK81_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNV32023.1"
                     /translation="MFDFQVHLDGAEYQGVVEAPAYVLLNKDWTSEGGRQVNRATAAV
                     RKSALPDTVSGKRVLATQETLDRVRASLGRSRLRWSPQREQIVMAFLKQDHITIRSLY
                     GVLNRNGRRAPLNTIYRTMRVLCDNGFAQTRRFGEETQYDNISAKGDHDHLICTGCGH
                     IVEFEDPVIEGLRQQVASVNGFHLTGRKLELYGLCSVCRVDASPVVRT"
     gene            9663..10181
                     /locus_tag="PKK81_03805"
     CDS             9663..10181
                     /locus_tag="PKK81_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV32024.1"
                     /translation="MEARTHLEMSTLIRDHGHDLQQFLTRRLGCTETAKDLVQDTFVR
                     LLQNRSGEVLTNPRAFLFRIATNLVIDHHRRRQYRETVTLDDPERPIDQADPAPSIET
                     VVWSKQQVARLKQAIEELPPKCRQVFVLIKFHHLSHAEVAVKLGISQSTVVKHMVKAV
                     DFCRSRLQDLSS"
     gene            10293..11282
                     /locus_tag="PKK81_03810"
     CDS             10293..11282
                     /locus_tag="PKK81_03810"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV32025.1"
                     /translation="MDTPERDRLASTASEWLVRMESGTITATERLRFAEWLSSDPSHR
                     DAYREAERFWHGLDRLNPDDIREFDRYLTEDTITDPRGTSRSWRRVAALAASLVLVAG
                     IGLWITTAWLPFGDYRTAVGEERTVTLADGSTVQLNTDTALSVSMTEQGRRLTLHRGE
                     AFFTVAPDAARPFEVEAGRGTIRALGTAFNVRTGSDRTTVTVTEHSIRIRLGKETSAE
                     VHAGERLRYRPDGWIDPVETADMDRVLAWRHHRLIFENQPLPEVLAELARYRSGRLVI
                     LRDQSLKTLPVTGSFDTERLDHLLPAIEESLPVRIVSFADRVILLYRGLPKKS"
     gene            11388..>12952
                     /locus_tag="PKK81_03815"
     CDS             11388..>12952
                     /locus_tag="PKK81_03815"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNV32026.1"
                     /translation="MFGTITTLCTPRDASPRPGTCITIHVLLCSLLLMLANHGSFVHA
                     QEPSSTVSRSFAIAPQPLSSALLQFSDATQIEVLFDATIARDLQTQGVSGDYAPEEAL
                     RMLLQDTGLSYRTTSAGSVTLEQAAGLPIVPAPTVQSPPTEPTPQAVSRPAAQKSVKV
                     PEIVVKDVRERDDDAQTYIAEESTTATRTDTPIRDVPQSIQVITRKVIEEQRTFRLQN
                     TLENVSGINATESAASLYDSLIIRGFSATDRSYFRNGLLDPFAQFTASDTYNIRRLEV
                     LKGPAAVLYGQGDPGGVINIVTNKPLPNAGYSANVTLGNFNFYRSELDATGPLNADKT
                     VLYRLNVAGQKAGSFIDYANRDLAAIAPSITWLMSSRTTLTVEADYLRRWSNDPYGLP
                     AQGTILPNFNGPIPRNRSVTLGDFSTFNRTSYRFGYDLTHQFNDKWSIRNAFRHTIVE
                     DDKNNLYAGGFFLEPDERTFQRFQVLQPAVARRHANSMVTNLVGHIRFLEMDHTLLTG
                     VELRQEKTDQFIFT"
     assembly_gap    12953..13052
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13053..13703
                     /locus_tag="PKK81_03820"
     CDS             <13053..13703
                     /locus_tag="PKK81_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV32027.1"
                     /translation="LFKPERSTQYEVGMKTFLFDNRVSATLAWFHLTRENLLTPNPDP
                     ALALQGFSVQTGEQRSQGVELDVTAQITPGWNIITGYAYTDAEVTKDNDLSLVAKRLA
                     NVPYNKFTLWSTYHFQEGPLQGFGFGGGLFAYSSRNSTIFEGQVDMPGYIRADAALYY
                     NRDLQKGNWLGAKGVNVALNIRNLLDQRYVATSYNGSSQFFFGEPRTVLATVGLRF"
     gene            13812..14066
                     /locus_tag="PKK81_03825"
     CDS             13812..14066
                     /locus_tag="PKK81_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32028.1"
                     /translation="MHYLALLVLLLSGCVEGRWVHPENTADQTAQDWEHCKAEVLSGQ
                     EHNKETLAGGINLSGCMHGKGYSYVEDRQPQAGADTSTHR"
     gene            14264..14848
                     /locus_tag="PKK81_03830"
     CDS             14264..14848
                     /locus_tag="PKK81_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV32029.1"
                     /translation="MALVGQAPYDALGAQATEQGPASTMESNPTNRTLGAYTGYYREL
                     LSFLTAQLRCPHEAADLVQETFARLLALDNPDVVRQPRAFLYRIARNLAIDSSRKVSV
                     RNRFLIDLSELEEAPSAAPLPDYLLEGNQLRRALDQTITEMPPRRRDVFLLYRFAGLK
                     QAEIAERLGISTSMVERHLMKAMAQCRQRLQSHL"
     gene            15189..>15467
                     /locus_tag="PKK81_03835"
     CDS             15189..>15467
                     /locus_tag="PKK81_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV32030.1"
                     /translation="MLLLVSGTLWWWSFAVTESTYRTAKGRQQTVTLSDGTILDLNTE
                     SSVTVRMWGRGRQVILHTGEAYFTVAKDPARPFEVVALNGHIRDIGTQF"
     assembly_gap    15468..15567
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15568..16063
                     /locus_tag="PKK81_03840"
     CDS             <15568..16063
                     /locus_tag="PKK81_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32031.1"
                     /translation="PFEVVALNGHIRDIGTQFSVYRQADRVLVAVESGAIDVVIPSTG
                     NEPSQAHVLSPGERAAYTTAGEWSRLDQIDPRRIAAWRQGTLRFDRIPLIDAIREIER
                     YWTGRILLADPALGSISVSGVFSHHNLAEFFTALPTIIPVQVTHRDGDIILTASMMPS
                     VRPQ"
     gene            16169..18631
                     /locus_tag="PKK81_03845"
     CDS             16169..18631
                     /locus_tag="PKK81_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNV32032.1"
                     /translation="MMTVRKCDAVQETRVTVSRWLRTLVRILSAVLAITGLAAAQADA
                     QDTVLSFDIPSQSLPAALDAFAEQAHVQMLYKADSVSDLQSTPVSGQLSAEQALHHLL
                     QGTGLSFQSGGPNTITVVTPPAEPNHESAPPARDRPRTEKPVKVPEILVKDVQEREGE
                     TSYVAEDTKTATKIDIPLIETPQSISVITRARMQAQEVNSIGEALRYTPGVRGEPFGF
                     DSRLTWLQFRGFDNTTNGLFKDGLQLRNPAFGISYNLEPYGAEQLDVLRGPASFLYGQ
                     GSPGGLLNYVSKRPTLQSLHEVQFLAGSFGRYEGRFDFGGRINDSDTFSFRMTGLIRE
                     SGTQIDNVPNDRVYIAPALTWRMASTTSLTFFATYQKDRAGAFQWLPADGTLGFNPNG
                     FVQPHRFTGQPSVDRYNRTEHSVGYEFHHQLSENWKFLQKMRYNSNETDNVSTYVLGL
                     EPDRRTAIRDQWSSYGKLTAIALDNQISGRLSTGPMQHTLLAGLDFQRISVRHNQNFA
                     EASSIDIFNRYDYGTLGVVPYFDSKQDTKQLQTGVYIQDQIKMFDKWLLTLGGRHDWA
                     RNEATDNVAGTTSTQSDRKATGRAALTYLFDNGLAPYFSYSTFFLPSIGIGADGKQFK
                     PESGQQYEAGIKYQIPRTRAFVTAAVFDLTRENYVQTDPGTFLPVQRGKARSRGLELE
                     GVASFDSGWDLIAAYTLLDNKVQETVDPSEQGKRLVQVPAQFSSLWAKYTVPDGKWKG
                     FGIGGGARYTGYNYADTPNAFRVPSYVVGDAVIDHTWNHYRFALNINNILNHNTFGCF
                     NEGLTCNFGERRTVVGSVAYRW"
     gene            18845..19246
                     /locus_tag="PKK81_03850"
     CDS             18845..19246
                     /locus_tag="PKK81_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV32033.1"
                     /translation="MKGYIVQIEDAMRDNQHYRRVLFTAKHSQLVLMNLKPGEEIGEE
                     VHGMDQFIRFEEGEGTVVLDGEKHAVSDGFAVVIPAGTRHNVVNSGKTAALKLYSLYS
                     PPEHKDGIIHKTKQEADADHDHHFDGRTSMK"
     gene            complement(19417..21264)
                     /locus_tag="PKK81_03855"
     CDS             complement(19417..21264)
                     /locus_tag="PKK81_03855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNV32034.1"
                     /translation="MTLSPSGEHIASIQNTAGDKTYLVVTTYDGKDARRLLESDNKRY
                     SIRWFDWVNDERLVVGVGADDGWSNLRWTETRLFAINRDGSNLKTDLITPSRDHNSFT
                     REYLPQLQDRVIGKIPGDNRSVLIALDLRIGTYPDVYKLDVYTGRRELIEGSTYGMRD
                     WMADRQGVVRLGTAVEGTMVHILVKPVGQDRWQTLRKYDALREPDMTPLGFDEDPNML
                     FVAQRLDGRTAVYSIDISNPQVPPTLRASHALYDIEGGLIYSSWLKQVVGVRSRLDES
                     ASIYWNPKAKALQTRVDLALPGRINDIQGSSRNGRRHIVVSSHATQPPQWYLYDEDLN
                     RLILMVDGYPKLVPNELVKPMPLTLKARDGTVLHGYVTRPAHAQQPGPLIVLPHGGPA
                     GRDVLDFNYWTQFLASRGWAVLQVNFRGSSGYGGEFLQAGFKRWGLEMQDDLTDAAHY
                     AIEQGIADPERICIVGGSYGGYAALMGIVKTPELFRCAVSFAGVSDLRALLTDKRRFL
                     GYELGAERQLGAWWSDRDRLKATSPVNHAEKIRTPLLIVHGAEDRTVPVEQSRAMVDA
                     LKEAGFKHMRYIELPDGDHHLSRQEDRLTFFREMERFLATHLDHAGGSE"
BASE COUNT         4617 a         6825 c         5824 g         4300 t
ORIGIN      
        1 ccatgggcca gattttttga caagcgaccc cacttccgca cggttagtga cctctgatct
       61 tcatcgcata gcacgagggc ccctgaccgc aaattgcagg ctgcgggtct ttgagagtca
      121 cgggaattcg ttccggtgca tctgcgagtc ggccaacctc catccatcgg tactgcgtcg
      181 ccttgcatcc gccgaatgac aagcgcctca ctcgaacaaa cctctcacac tttcatacct
      241 atcttttccc actgagcagt ttccggatac tgaacattcc acgcacgaca gatattggct
      301 aggatcgccc catgctcgac ctcgtcggat tgggtatcct cgcaggagtc gttccggttt
      361 acctgggcat cgcggcggcc ttggtgcttc gtcgtgccct tccccggacg tgggaagccg
      421 gcctgatcgg gctctccacc ggggtactga cctatctctt cttcgacttg atgcacgaag
      481 cggtggaatt gatgggagtg cgcgatccct tctcgtggct gctgtttctg ggcagcctgt
      541 ttctcagctt tgtcggcctt gtccacctcg aaaaacatca attcctcaaa ggacggcacg
      601 ccgaccaggc actgacactt ccgtttgtca tcgcggcggg catgggtctt cacaacctgg
      661 gtgaaggact cgcgatcgga accagttacg ccggcggaga atgggccttg agcggactcc
      721 tcgtggctgg tttcgccctc cacaatggaa ccgaaggatt cggcattgtc ggggcggcgg
      781 ggcaatctcc gatccctccg cgcacggtgg tgttactcgg cttgaccggt ggcgcgccta
      841 catgtatcgg aacggtccta agtggactca ccgtgtcgcc ctacctttct ctcgtctggt
      901 atgcgttagc cgccggatcg ctcttatatg tcgtgtttgc cttggtggcg atgacataca
      961 cgagctcccg caaggtccat atggcctatg gtgtctggac gggtattgca cttatggcga
     1021 tgacggccat gtttctgaca ttagtcggcg gacatcgctc ctaatcctcc gctccgttcc
     1081 ggacaaattt tcaaacgatc actcggtggc ctgacgcttt gctccacagc ccgcttgaca
     1141 tgtttttcta aattgtgata ttgagaatcg tactcaataa cgagatagtc tctgccagta
     1201 ctctcagcag aggcccgagg aatttaattc ccagagccca cggtcatacg atcgcgggct
     1261 ttttattttt tgtgggggaa attatgcacc aagaccgccg ggcacgaact atcgatacat
     1321 ccatcatcgg catcgtctca tccgggagcc ctccgctttc aatcgaagcg tcacggcaac
     1381 ggccattgca cagcctgcgg accaagccac aacctttctg gccgacgcca cagatcacgc
     1441 ctctcaattc cgttatcccg gcggcacgct tgtcggccct gctgtccaag cgcagcgggg
     1501 atcgcctgac gttgaaggaa ctctccactc tcctggggta ctcggagaaa tacacctccc
     1561 agatctttcg caagtatatg gggctctcct tttccgaata cctcaagcaa ctgcatctcg
     1621 ccaaagcaac gaaactcctc agcgaccaga gcctgaccat cgcccagatt gcggaatcgg
     1681 tgggattcag cgacgcgttc gcgttcagcc acttcttcaa acgagccatc ggatgttcgc
     1741 cgagcgaatt tcgaaaacaa cagacgccgg aagcaggttt gagatgaacg acaaccgcga
     1801 gtctctggcg cgaatgggag gatggctggt ttcgctgacc ctgcatggcc tgagtctcgg
     1861 gacggcgttg gtcctcgcgg ctgaattctc cattctcccg gagccgcgcc ccttccgctg
     1921 ggaagttaca atggtgagcg cgccggcgtt gcaacctgtc gcagcggacg tacccacccc
     1981 cttttccgca tcgtccgtct ccccttccgt catgccgagg gacaagtctc cacggcaggt
     2041 ccggtcccgt ccgaacgccg ccaataccga agcggcccgc ccgctggaga ctcattccgg
     2101 aatcatcgca cctgcgcaag atggattcgt ccgcccacta gccaccgaga acccctccct
     2161 ggtcgtcgac agtgtctcgc ctggccctca tacaccatca gcggacctga catccgacga
     2221 actagtgcac gaggcgcctc aagtcacacg tgtagatctt ccggtcacca cgacggagcc
     2281 gtccgaaata ccgcctccga gcgtcgatgc agccgaagta tcccacacga cctccatgcc
     2341 ttccattcgt gaatcgatcc ggctcgtcaa ccgtccgaca cctcgcgccc gcgaggccat
     2401 cgtctcacgg atactccatg ccgattacgg atggctcgcc gaaatgttgt tcgcaaaggt
     2461 cgaacagagc aaacgttacc caacattcgc caaaagccgt cgctggcagg gcaatgtcat
     2521 gcttcaagcc gtgatccatg aagacggcag catcagcgac attacgatcg tcacgccatc
     2581 gggccactcg attctcgatc tcgacgcagt cgctctgctc gaacaagcgt ctccggtcca
     2641 gttgaagtat ccactcggtc aatcccatgt tgtcgtccac atccccatcg gctatcgcct
     2701 ggaataggat ccatcatgca gaatgaacat acacttccct gtctcactgc cttcgaagag
     2761 catcacaacg atctcttgag gttcttcacc cacaagctcg gctgccgtga tctcgccgcc
     2821 gattgcactc aggataccta catgcacctc gtccgcatga ggccttcaat cgatgtgcaa
     2881 aacccgcgag ccttcctgtt tcgggtcgcg gccaatctcg cggtagacaa tctgcgaaaa
     2941 cttcgcaccc gtcggggggc gctcagcatc gaaccgcccc ctgaagaaac agccagtccg
     3001 gccccgtcgg ccgaggatac cttggacgcc aagcagcggg tcgcccaatt ggaagtcgcc
     3061 atgcgcgaac tgtcgcccaa atgccggcgg gccttgctgc tcaacaggtt ggaagggaaa
     3121 acacaccggg aaatcgccga cagtctgaag gtgtccgaga gtatggtcgc gaaatatatc
     3181 gtccaagcct tgcggcactg ccgcgcccgc ttgcatccgg aggaatccac cagatcatcc
     3241 taactgttga aagccgagcg ccatccgtct tatagagtag cgtacatcga ccagtatccc
     3301 gttcaggggc agtcgtgtgc acaatggtac gatggatgcc tgactcgaca cctccagatg
     3361 cctcctctcc actgatcgac gaagccctcg cttggttggt ccgcatccat tccggtcacg
     3421 cctccgatga agagcgcctg gcgtgcactg cctggcggac gcaaagttcc gcccaccaac
     3481 tcgcctacgc agaggcggaa atcctatggc gggatatcgg cttgctgccg aatggcgatt
     3541 gcgccggttc ggccgacctg acggcatcga tgccgcacgg cacgctcccg cgtgcacgac
     3601 gcacgatcaa gtggggactg gccgcttgcg ttttactcgc cgccggttgg ctctcatggg
     3661 gaatcgttca agaacgactc gacctcgcgt cagccgatta ccgcaccgca gtgggggaac
     3721 aacgacacgt caagcttgag gacgggagtc gtatttatct caacaccgat accgcagtgg
     3781 ccgtcgcctt cactccgcag cagcgaacga tccggctgct caccggagaa gccagtttca
     3841 ccgtcactcc cgagacagta cggccgttca cggtacagag cggagatgtg agcacccgcg
     3901 cgctcggaac aagctttctg gttcagcgcc accaagatgc cgtcaccgtg accgtcacgg
     3961 agcatgccgt tcaggtatcg gcaacggacg gccgccaggt tcccgcagcg gtggtccggc
     4021 aaagcgagca gatctcctac agtgccgagg ccggactggg tagggcccac gccatcgact
     4081 ccaatgccat cgccgcctgg caacgtggga aaatgatctt cgaagcccag ccgctggccg
     4141 cagtcgtcga agagctcaac cggtaccggg ccgggcgcat tatgattctg aacccggcgc
     4201 ttcgctcact gaacgtgaca ggtgtgttcg acattggcga cccggatgcc gcgctccgaa
     4261 tgattgaaca gacgttgcga attcacgaca ccaccctgac gccctacctc gtcctcttgc
     4321 actaacagac accgaagttt tcctgagacg tcatccatcc tcttcgcaat tagacgctgt
     4381 cttccgtcat ctccttcaag ttttttttca acgcacacga tttcgattac aagtttttcg
     4441 gcccggtccg tctttaccta atgaagcgcc gagtgcgcgt cgctcggccc gtgcccggat
     4501 acgtgcgctt attcataccg gactaataga gggggaatga tgcatctctc atggcaagtc
     4561 gtcgtcacac tctccgtatc ggccgttctg gtgcatacgg ccggaaacct ggctgaagcg
     4621 cgaagcgagc gtctcaccac atcgaacaac agtccccttc tccttgcgca ggccacagca
     4681 gctgctgaga catcagacac ggtggacttc aatattccac cgcaggcctt ggggtccgcc
     4741 atcaccgcat ttggcgatca agccaactat cgtctcttgc tgccatccga cctggctgaa
     4801 ggaaggagca ccagcggcgt cagcggtcgt cacacgccgg cggaggcctt ggcgctgatg
     4861 ctcgctggaa ccggccttag ctatcgaatg accgaggccc gaacgatgac gttggaaccc
     4921 gtggcggcaa cgcccttgcc tctcgctccg ctgagccaag agccagcgcc gcccaacccc
     4981 aacacccaga ttcaagcggt acccaagccg gtgaaagtgc cggaaatcgt tgtgaaggaa
     5041 gtcgaagatc gcgggtatgc cgtcgacgaa tcctcttcgg ccacgcgtat tcccgctccg
     5101 atccatgaca caccacgctc cgtggaggtc gtgacccgac aggtcctgga cgaccaaaaa
     5161 gtgattcgct tcagtgaagc cctccggaac gtcagcggcg tctcgcagtc cagcacgcaa
     5221 ggcggacaag gcggtacctt catgattcga ggctttgcct ccgatctgaa tgtcttcaaa
     5281 aacggcttcc gagacgacag taccttcagt gcccgtgcgc aacgcgatat catcaacatc
     5341 gagagcgtcg aagtcgtaaa aggtccgccg tcctacctgt acggccgctc tgatcccggt
     5401 ggagtcatca accaggtcac caaagctccg ttgagaaatc cctactattc ggcggagatg
     5461 atcgtcggca gttacgggct gtaccgcccc acgatcgaca tcggtggtcc cctgaacgaa
     5521 agcaagacgc tcacctaccg cttcaacggc atgtacgaat cggcggagag ttatcgagat
     5581 ggcgtgaaga ccgaacgtat cttcctcgcc ccgaccttcg gttgggaaat gagttcacgg
     5641 acgacgttgc ggttcgaagc cgaatacatg tacgaccgcg cgcctatcga tcgagggctc
     5701 gtcgccatcg gcaacggggt cgctccgata ccggtctcgc gcttcttggg tgatccctcc
     5761 cgcaagctgg agacccatca tgggaaggcg accattactc tgtggcatga catcagcaat
     5821 atgttccggt ggcgaaccgc gttcagaacc gccgtcacca gcagccgcta ttccagcctt
     5881 gaatccaact tcctggtcgg agccgagagc gacggcatct tgaatctggc aaggtatgag
     5941 attccgacga cggtccagag ccactacttg cagaatgaac tccatggcaa cttcacgacc
     6001 ggctcgatca aacataaaac gatcatcgga atcgaactcg gtcgagaaaa ctcatcggcc
     6061 acggcctcgg gggatttcgg cggcgacacg agcacgccgg gcgcgttcag ctatatcaac
     6121 atcttcaata ccaacgaccg gttattcttg aatccggccc tcacgaaatt cagtgacgcc
     6181 agcacgcaga acaacatcct gggtgcgtac ttcggcgacc aagtggacct gttggataac
     6241 ctgcatgtgc acttcggcgg acggttcgac ctcttcgatc aaaccatcac gaatcatccc
     6301 gacgatttta ccgccaccag cagccaaaac aacaaaaccg attcggcctt tagtccctca
     6361 gtcggtgtgg cctatcaacc ctggaagccg attaccctct ttgccaacta tacggagtct
     6421 ttcgcgccgc aaagcgcagg ttccaggagc atcaacggca acctattcaa tccggaacgc
     6481 ggcaagtcct atgagggcgg catcaaatat caggcgttcg gaggacggtt acgttcgacg
     6541 gtcgcgctct tcgataccag gaagaagaat gtgctcacgg ccgatccgct caacggcttt
     6601 ttcttctccg ttgcgacggg agaacaacgc agcaaaggcg tggagttcga catcgcgggc
     6661 cagatcctgc ccggatggga catcattgca aactatgcct acatcgacgc gcgtgtgacc
     6721 aaggatctcc tgttcgccga ggggagtcgg gcgccgaaca gcgccttgca ccagggaagt
     6781 ttgtggacga cctatttttt ccaggaaggt gtcgtgcagg gtttcggcgc cggtatcggg
     6841 atgtacgcac agggaaagcg aaacggaatt ttccagtgtc aggatcccgc caattgccaa
     6901 gcaccattcg agatggccgg gtatgtccgc atggacgccg cgctctacta tcggaagcag
     6961 gaagtcttca acaagaccaa cctgttggca gccatcaatt tcaccaacgt tctcgaccat
     7021 cgctatttca gcggtgcgca aaacttccgc gagatcgtgt acacgggcgc gccgtttaca
     7081 gccatcggat ccctcaagtt cgagtttcac tgacctcgtc gcccttccga ctgccgcaac
     7141 cgcaacaggg gagtctcaca tcgagactcc cctcctcctt tcatgatccc acgggccggc
     7201 tcacagacac aggcaatcct cgagccgcag ctcaccccag agccgccgcg acgaagcacg
     7261 tcatcagtct ggttatttca cggaatcaga ggacggacag gtgaggtatt tcttgaactt
     7321 tgaaagggga aagaccacca gctctccgca acgatggcac ttcacgacca ctcctcgctt
     7381 ctcgagccga gccgtgagtc tgccacagag acaccgtatg tctttccccg tctgagagac
     7441 tgccacttta ttcaccatgg ttggtttcta cccacgcagg cggtcactgt cccttcttat
     7501 taggacgcat gagcgacaca aaaccttacc ctctgggtcc ctgtctccag accgatccgc
     7561 ccttgccctc cttgtggctc caaggtgtgg tttcaggctg gtcacgcgtc ttattaaaaa
     7621 ggggctcatg gtgttcgcca caggcgcgaa tctcctgcac cggccgccga gggagaccgg
     7681 agacagacaa tgatcgtcag ccagctccaa cccgtgcgcg tgccttcaaa cacgtcccgt
     7741 ccgcagactt ccgcgccccc agcggtcgcg gccttgcccc agcctgcccg caaggtttgg
     7801 cgaaaggtgt ggctaaacct gcacctctat gtcggcctgt tgggcggcgc gctcttcgtg
     7861 ctcaccagcc tcaccggcag cctgttggtg ttctacaaga cgatcgacga atggatgaat
     7921 ccggagcagt tgatccggac tgccggcagc gatcaatccc ttagcgacat cgtagcgggc
     7981 gcccggaccg tgcatccgga ctggtctccc cccgacaccc tcatctttcc gctccacgag
     8041 cgtgacacgt tccatgcctg gttcaaggat cctgctgcca cacctcccga agagcgctgg
     8101 cacgtcgttg ccgtcgaccc atcgaccgcc cgccccctga gtgaccggca gtggggcagt
     8161 tttttcgtct cgttcattta cgaattgcac caggagctct tgctcgggaa gccaggcgag
     8221 gtctttgtcg ggatcatggc cgtttttctc ctcgtttcga tcgggaccgg gctgtatctc
     8281 tggtggcccg ctctgggaaa actccgccgg gccttgtcgt ttcaatccgg cgggagtccc
     8341 atcagaagac attacgatgt acacaagctc accggcctgg ccggtaccct cttgctgact
     8401 ctcctcgccg tgaccgggtt ctatttggaa ttccccgacg ccgtcacctc agtcgtgcgc
     8461 tggttctcac ccgtgcggga cacgtcgccg gaactacaac ctcaatccga accgcaaacc
     8521 ggattgccca agcttccgcc cgatcaggcc atcgcaatag ctcgaaccgt cttccctgac
     8581 gcgaaaccga tgtggctcgg cttgcctcag catgacaggg acagttattc ggtggggctc
     8641 cgtcaaccgg gagaggtccg acaggcggga gggcaaaccg aggtgtggat cgatcaatac
     8701 agcggcgcgg tacggcgggg ccaggactgg agacagttca cccgcgggga aaccctgttg
     8761 tcatggctct tccccctgca caacggtgaa gcgttcgggt tgaccggccg ctggatcatc
     8821 ttcttcgccg gtttgacccc gctgctactt tatctgacgg ccctgcgcat gtggtggttg
     8881 aagcgtgttg cgcaccaacg ccggcgagag gcctgaacca acgggcggag agtgaatcca
     8941 ttccgccgtg ctcaagtccg aacgaccgga cttgcgtcga cgcggcacac cgaacagagt
     9001 ccatacaatt cgagtttgcg gccggtgaga tggaagccgt tcaccgacgc gacctgctgc
     9061 cgcaaccctt cgatgaccgg gtcttcaaac tcaacgatat gaccgcagcc tgtgcagatc
     9121 agatgatcgt ggtctccttt cgctgaaatg ttgtcatact gcgtttcttc gccgaagcgt
     9181 cgcgtctgcg cgaacccgtt gtcacacaac acccgcatcg tccggtaaat cgtattcagc
     9241 ggggcccggc gcccgtttcg attgaggacg ccgtacaagc tgcggatggt gatgtgatcc
     9301 tgtttgagaa aggccatgac aatctgttcc cgctgtggac tccatctcaa acgggaacgc
     9361 cccaggctcg cccgaacccg gtcgagcgtt tcttgagtcg cgaggactcg ctttccggac
     9421 acagtatccg gaagtgccga tttcctgact gcagcagttg cacgattcac ctgtcgcccc
     9481 ccttcgctcg tccagtcctt gttgagtaag acgtatgcgg gggcttcgac cacaccctga
     9541 tattcggctc catccaaatg cacctggaaa tcgaacattc ccttttgcgt ctgcccgcca
     9601 ctactatcca accgccgcga atcacgatcg cacaaccctg ttctgcgacg gaaccatact
     9661 gaatggaagc acgtacccac ctggaaatgt cgacgctgat ccgagatcac ggccacgacc
     9721 tgcagcaatt cctgactcgc cgcctgggct gcacggaaac cgccaaggac ctggtccagg
     9781 atacgtttgt tcgcctgctg cagaacaggt ccggcgaggt cctcaccaat ccgcgcgcct
     9841 ttctctttcg catcgccacg aatctcgtca tcgaccacca tcgccggcga caataccgcg
     9901 agaccgtgac gctcgacgat cctgaacgcc cgatcgacca ggcagatccc gcgccctcga
     9961 ttgaaaccgt cgtctggtcg aaacagcagg tcgcgcgcct gaaacaggcg atcgaggaac
    10021 tgcctcccaa gtgccggcag gtgtttgtgc tcatcaagtt tcaccacttg agccatgccg
    10081 aggtggccgt caagctgggc atttcccaaa gcaccgtcgt caagcacatg gtcaaggcgg
    10141 tggacttttg cagaagccga ctccaggatt tgtcatcctg aacgtcccgc cagaatggat
    10201 gttctccccg cctcgcccgt ctactgttag tgggaacctg tcgaaccgga agatgacagg
    10261 gcaacgggcg acgcgctacc atgatcaccc caatggacac accagaacgc gacaggctcg
    10321 cctcgaccgc atccgagtgg ctggtacgga tggagtccgg cacgatcacg gccaccgagc
    10381 ggctgcgctt cgctgaatgg ctctcctccg atccttccca tcgagatgcc tatcgggagg
    10441 ccgagcgatt ctggcacggg ctggaccgtt tgaatcccga cgacatccgc gagttcgatc
    10501 ggtaccttac agaggacacg atcacggatc cgcggggaac atcccgctct tggcggcgtg
    10561 tcgcggcgct ggcggcaagc ctcgtgctcg tcgcaggcat cggtctctgg atcacgactg
    10621 cctggttgcc gttcggcgat taccgcacgg cggtgggaga agagcgaacg gtcaccctgg
    10681 ccgatggatc gaccgtgcaa ctgaatacgg atacggcatt gtccgtgtcg atgacggaac
    10741 agggacggcg gctgacgctc catcggggcg aagcgttctt caccgtcgcg ccggatgcgg
    10801 ctcgcccctt tgaggtcgaa gccggcaggg gcacaatccg ggcgttggga acggcgttca
    10861 acgtgagaac aggctccgac cgcaccaccg tcacggtcac cgaacacagc atccggatcc
    10921 gcttggggaa agagacgtcc gccgaggttc acgcgggaga acgccttcgg tatcggccgg
    10981 atggatggat cgatccggtc gaaacggcgg acatggaccg agtactggcc tggcgccacc
    11041 atcgcctgat cttcgagaac caacccttac cggaagtcct cgccgaattg gcacgatacc
    11101 gttcaggccg gctcgtcatc ctccgggatc aatccctgaa aaccctgccc gtcaccggct
    11161 cgttcgacac cgagcgattg gatcacctcc tgcccgccat cgaagaaagc cttcccgtcc
    11221 ggatcgtctc gttcgccgat cgcgtcatcc tcctgtatcg gggcctcccc aagaaatcct
    11281 gaaattttct tcgagcacta gacctttcct ccctcccgtc cgtcgtagtg aatgggccgt
    11341 gaagacggcc gcatcaccca tcgcaccacg acggaaagga ccaagcaatg ttcggaacca
    11401 tcacgacgct gtgcacccct cgggacgctt cacctcgacc aggcacctgc atcacgattc
    11461 acgtactgct ctgcagcctt ctcctgatgc ttgcaaacca tggctccttc gtccacgcgc
    11521 aagagccgtc ttccacggtc tcgcgatcct ttgcgatcgc cccgcagcct ctctcttccg
    11581 ccctgcttca gttttcggac gcgacgcaga tcgaagtgct gttcgatgcg accatcgccc
    11641 gcgacctcca gacgcaaggc gtctcgggag actatgcccc ggaggaggcc cttcgcatgc
    11701 tgttacagga cacaggcctc tcctatcgca ccacgagcgc cgggagcgtc acactcgagc
    11761 aggccgccgg cctcccgatc gtgccggctc cgacggttca aagtccgccg acagaaccca
    11821 cgcctcaggc tgtgtcgcgc cccgccgctc agaagtcggt gaaggtcccg gagatcgtgg
    11881 tgaaggacgt ccgtgagcgc gacgacgatg cccagaccta tatcgccgag gaatccacca
    11941 cggccaccag gaccgatacg ccgattcgag acgtgccgca gtcgattcag gtcatcacgc
    12001 gcaaggtcat cgaagaacag cgcaccttcc ggctccagaa cacgttggag aacgtgtccg
    12061 gcatcaacgc cacggagtcc gcggcctccc tctacgactc gctcatcatt cggggcttca
    12121 gcgcgacgga tcgtagttac tttcgcaacg gcttgctgga tcccttcgcc caattcaccg
    12181 cctccgacac gtacaatatc cgccggcttg aagtcctgaa gggtccggcc gccgtcctct
    12241 acgggcaggg agatcctggc ggtgtgatca acatcgtgac gaacaaaccc ctgccgaatg
    12301 caggctattc agccaatgtc acgttgggaa acttcaactt ctaccgatca gagcttgatg
    12361 ccaccggccc gcttaatgcc gataagaccg tcttgtatcg attgaacgta gccgggcaga
    12421 aagccggcag ttttatcgac tatgccaatc gcgatctcgc ggccatcgcg ccgagcatca
    12481 cctggctgat gagctcacga accacgctga ccgtcgaagc cgattatctc agacggtgga
    12541 gcaacgaccc ctacggcctg ccggctcaag ggaccattct ccccaacttc aacgggccca
    12601 ttccacgcaa ccgttccgtg accctcggcg acttcagtac gttcaaccgc acgtcgtatc
    12661 gcttcggata tgacctcacc catcagttca acgacaaatg gtccattcgc aatgcgttca
    12721 gacataccat cgtggaggac gacaaaaata atttatacgc aggcggattc tttctggaac
    12781 cggacgagcg caccttccag agatttcagg tcctgcaacc ggccgtggcc cgccggcacg
    12841 ccaattcgat ggtgaccaac ctggtaggcc atattcgctt tctggagatg gaccatacgc
    12901 tgttgaccgg tgtcgaattg cggcaggaga agaccgatca attcatcttc acnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctgttcaa gccggaacgg tcgacccaat
    13081 acgaggtcgg tatgaaaacg ttcctcttcg acaaccgcgt gtcggctacc ctcgcctggt
    13141 tccacttaac ccgtgaaaat ctactcacgc ccaaccctga tccggcgctg gcgctgcaag
    13201 gattctcggt tcagacgggt gaacaacgca gtcagggcgt ggaattggac gtgaccgccc
    13261 aaatcacccc ggggtggaac atcatcaccg gctatgccta taccgacgcc gaggtgacca
    13321 aggataacga tctgtcactg gtggcgaaac gactggccaa cgtgccctac aacaagttca
    13381 ccctctggtc gacgtaccat tttcaggaag gtccgctgca gggattcgga ttcggcggag
    13441 ggctgttcgc ctatagcagt cgcaattcca cgattttcga agggcaagtg gacatgcccg
    13501 gctacatcag agccgatgca gccctctact acaatcggga tctgcagaag ggcaactggc
    13561 tgggagcgaa aggagtgaac gtcgcactca atatccgcaa ccttctggat cagcgatacg
    13621 tcgccacctc ttacaacggc tcgtcgcagt tcttcttcgg cgaaccccgg acggtgctag
    13681 cgaccgtggg gctgcggttc tgacctcgtc ccggtacgga atacagaagg cgggccaccg
    13741 gtgttatccc gtggtccgcc ttacctgcca tcgccgggga aacaacggac gattctctga
    13801 aaaggaattc tatgcattac ctcgcactcc tggtcctcct cctgtccggc tgcgtcgaag
    13861 gccgatgggt tcatccggag aataccgcag accaaaccgc acaggactgg gaacactgca
    13921 aggcggaggt gctctcgggg caggaacata acaaagaaac cctggcaggc ggcatcaacc
    13981 tcagcggctg catgcatggc aaagggtatt cctacgttga agaccgccag ccgcaggccg
    14041 gagccgacac ctcgacgcat cgctgacggc atcggtctgc acgaacggat acgccaccgc
    14101 ctggcactcc cgatcggtcg ttcctcttca gatacagcct ctctcctcat cgccaaatgg
    14161 gccgagcaga aagaccctct gcttcctgcc tggaggttgg gtttttcagc tctcgcccgt
    14221 cacccttttc caggaacaac gcacaacatt caggagtgta gacatggccc ttgtgggcca
    14281 ggccccttat gatgcgctcg gcgctcaggc aaccgagcaa ggacctgcct caaccatgga
    14341 atccaatccg accaatcgca cactgggcgc atacaccgga tactatcggg aacttctaag
    14401 cttcctgacg gcccagctgc gttgtccgca cgaagcggcg gacctggtgc aggaaacctt
    14461 cgccagactt ctcgcgctcg ataacccgga cgtggtccgc cagccacggg cgtttctcta
    14521 tcgcattgcc cgcaacctcg ccatcgactc gagccggaaa gtttccgtcc gcaatcgttt
    14581 cctgatcgat ctcagcgaac tggaggaagc cccatccgcc gctccgctgc ccgattatct
    14641 gctcgagggg aatcagctca gacgcgcact ggatcagacc atcactgaaa tgccgcctcg
    14701 ccggcgggat gtctttctgc tctaccgctt cgcagggttg aagcaggcgg agattgccga
    14761 acgactcggc atctcgacct cgatggtgga gcgacacttg atgaaagcca tggcgcaatg
    14821 cagacaacga ttgcagtcac acctgtgaca tctctaccac cagccaaagg ccttgatcgg
    14881 tcatgaccga ttcgttgcct ccatctcgac gaactgcagc cgaggacgcc atccgatggt
    14941 ttctccgcct gcgtgaaccg ggctgttctg ccgaggacac agggctcttc gaacaatggc
    15001 tggcctccga ccctgcccac cacgcagagt acctggcggt acaggcgacg tggaaacacg
    15061 tggatgccgt tgcgactcga cccagtcccg aattgcaatt ccacctcgcc cacgcgatga
    15121 ccatccaagt gaaatcgcag agagaacatc gaggcaggat cctcagtcaa ttgacagcgt
    15181 tggcggcgat gctccttctg gtgagcggaa ccctgtggtg gtggtccttc gcggtcaccg
    15241 aaagcacata ccgcacggcc aaaggcaggc aacagaccgt caccctctcc gatggcacga
    15301 tcctggatct caataccgag agcagcgtca cggtacgaat gtggggccgg ggacgccagg
    15361 tgatcctcca caccggcgag gcctatttta cggtcgccaa agatccggcc cggccgttcg
    15421 aagtcgtcgc actcaacggg cacatccgcg acatcggcac gcagtttnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gttcgaagtc gtcgcactca acgggcacat
    15601 ccgcgacatc ggcacgcagt tttcggtcta ccggcaggcc gatcgcgtgc tggttgccgt
    15661 cgagagtgga gcgatcgacg tggtcattcc ctccacaggc aacgagcctt cacaagccca
    15721 tgtcctgagc cctggagagc gagccgccta cacaaccgcc ggcgagtggt cgcggctcga
    15781 tcagatcgac ccgcgacgaa tcgcagcctg gcggcaaggc accctccggt tcgaccggat
    15841 accgttgatc gacgccatac gggaaatcga gcgctactgg actggacgga ttctcctggc
    15901 cgatcccgcg ctgggaagca tctccgtgag cggggtgttc agccaccata atcttgcaga
    15961 attttttacg gcgctaccga ccatcatccc ggttcaggtc acccatcgcg acggagacat
    16021 tatcctcact gcgtcgatga tgccgtccgt gcgtccgcag taatccccga caatttcacg
    16081 tgaacaggtt ggggaccgcc cccctcactc gtcttctgta cagatcaaga agaacgttca
    16141 ccaccacaga actgcgcggg ggagcacgat gatgaccgtg aggaaatgcg atgccgtaca
    16201 agaaacgcga gtcacggtga gccggtggct tcgcacactc gtccgaatct tgtcggccgt
    16261 gctggcgatc acaggcctgg ccgctgcaca ggcggatgct caagatacgg tgctctcgtt
    16321 cgacattccg tcccaatcac tgcctgcggc gttggacgct tttgcggagc aggctcatgt
    16381 ccagatgcta tacaaggcgg acagcgtgag cgatctgcag tccacgccgg tgtcgggtca
    16441 attgtcggcc gaacaggcgc tgcaccacct tctgcaaggc acggggttgt cgtttcagtc
    16501 cggtggtccg aacaccatca cggtcgtgac cccgcctgca gaaccgaatc acgaaagcgc
    16561 gccgccggcg agggatcgcc ctcgcacaga gaaaccggtc aaagtgcccg agatcctggt
    16621 gaaggacgta caggagcgcg agggtgagac gtcctatgtg gcggaggaca cgaaaaccgc
    16681 caccaaaatc gacatcccgc tgatcgaaac gccccagtcc atcagcgtca tcacgcgcgc
    16741 ccgcatgcag gcgcaagagg tgaactcgat cggcgaagcc ttgcgataca caccgggcgt
    16801 acgaggcgaa ccgttcgggt tcgattcccg cctgacctgg ctccagtttc gcgggttcga
    16861 caacaccacg aatgggcttt tcaaggacgg tctgcaactc cggaatccgg ccttcggcat
    16921 cagttataac ctggaaccct acggcgcgga acagttggat gtgctccgtg gaccggcgtc
    16981 ctttctatat ggacaaggga gtcccggcgg cctcctcaac tatgtgtcca aacggccgac
    17041 actgcaatcg ctccacgagg tgcagtttct ggccggcagc ttcggccggt acgaaggccg
    17101 gttcgatttc ggaggccgga tcaacgacag cgacaccttc tccttccgca tgaccggcct
    17161 gatccgggaa agcgggacac aaatcgacaa tgtgccgaac gatcgcgtgt acatcgcacc
    17221 ggccttgacc tggcgaatgg cttccactac cagcctgacc ttcttcgcca cctaccagaa
    17281 agatcgcgca ggggctttcc aatggttacc ggccgatgga accctgggct tcaaccccaa
    17341 tggattcgtt cagccacatc ggtttaccgg tcaaccttcg gtcgatcgat acaaccgcac
    17401 cgaacattcc gtcggctacg aattccacca tcaattgtct gagaattgga agttcctgca
    17461 gaaaatgcgc tacaactcga acgaaacgga taacgtctct acctacgtcc ttggactgga
    17521 accggaccgg cggacggcga tccgagacca atggagcagc tatggcaagc tgaccgccat
    17581 tgcgctcgac aatcaaatca gtggaagact ctcgaccggc ccgatgcaac ataccctcct
    17641 ggccggcctc gattttcaac ggatctccgt gcgccacaat cagaacttcg cggaggcctc
    17701 atccatcgac atcttcaatc gatacgacta cggcacgctc ggagtcgttc cctatttcga
    17761 cagcaagcag gatacgaagc aattacaaac cggcgtctac atccaggacc agatcaaaat
    17821 gttcgacaag tggttactta cgctcggagg ccgccacgat tgggcgcgga atgaagcgac
    17881 cgacaacgtc gccggcacca ccagcaccca gagcgaccgc aaggcaaccg ggcgcgccgc
    17941 cttgacctac ctcttcgaca acggcttggc gccctatttc agctattcga cttttttcct
    18001 accctcgatt ggaatcggcg ccgacgggaa gcaattcaaa ccggaaagcg gccagcagta
    18061 tgaggccgga atcaagtacc agatcccacg cactcgcgcc tttgtgacgg cggcggtatt
    18121 cgatctcacg cgggaaaact atgtgcagac cgaccctggc acattcttgc cggtacaacg
    18181 cggcaaagct cgctcccgcg gcttggaact ggaaggtgta gccagtttcg attccggttg
    18241 ggacctgatt gccgcctaca ccctgctgga taacaaagtg caggaaacgg tcgatccgag
    18301 tgaacaaggc aaacgtctgg ttcaggtacc ggcacagttc agttccttat gggctaaata
    18361 caccgtgccc gacgggaagt ggaaaggatt cggaatcggc ggcggggcga gatataccgg
    18421 ctacaactat gccgatacgc ccaatgcgtt ccgggttccc agttatgtgg tcggcgacgc
    18481 cgtcatcgac cacacttgga accattaccg gtttgcgctg aacattaaca acatcctgaa
    18541 ccacaatacg ttcgggtgtt tcaacgaagg cctgacctgt aacttcggcg aacggcgcac
    18601 cgtggtgggg agcgtggcgt atcgatggtg agaagcatca gccggaaccg gcatattgac
    18661 tccccttcag gattcagcaa acaggagtgc cgttcctgcg actcgtcggg acgtattggc
    18721 cgctcacggg catcgtgttt gagatgaact catcatgatc ctgccgacta cccacctcat
    18781 tcagcgcagc cgacgggtca accgcgcact tgtctgaagc aattacgcac gaaggacgtt
    18841 ccccatgaaa ggttacatcg ttcaaatcga ggacgccatg cgcgacaatc agcattaccg
    18901 ccgggtactc tttacggcga aacactctca actggtccta atgaacctca agcccggtga
    18961 agaaatcgga gaagaagttc acggcatgga ccagtttatc cgattcgagg aaggggaggg
    19021 aaccgtggtc ctggacggag agaaacacgc ggtgagcgac ggctttgcgg tcgtcatccc
    19081 ggccgggacc aggcacaatg tcgtcaatag cgggaaaacg gcagcactta agttgtacag
    19141 tctctatagc cctccggaac ataaagatgg aatcattcac aaaacgaagc aggaagccga
    19201 cgcggatcac gatcatcatt ttgacggtcg gacttcaatg aaatagggct gtttacgggt
    19261 catatcctcg gcgcgcgggc gagtaactcc atgagctgac gagagactcg tcgggccgct
    19321 gcacgaaggt cttgtcgaag gccagccctg cctcgctctg caaacctgcc ggacagtttg
    19381 aatcggacct ctccccatca gcatacatgg tcaagttcat tcagaccctc ccgcatgatc
    19441 gagatgggtg gcgagaaacc tctccatttc acggaaaaat gtgagacggt cttcttggcg
    19501 gctgaggtga tgatcgccat ccggcagttc gatataacgc atgtgcttga aacccgcttc
    19561 tttcagagca tcgaccatcg cgcgagattg ttcgacaggc acagtccgat cctccgcccc
    19621 gtgaacgatc aagagcgggg tgcgtatctt ctcagcatgg ttgaccgggg acgtggcctt
    19681 cagccgatcg cgatcggacc accaggcgcc taactgccgc tccgctccca gctcataccc
    19741 caaaaaccgc cgcttgtccg tcagcagcgc gcggaggtcc gacactccag caaagctcac
    19801 cgcgcaacga aagagctccg gtgtcttgac gattcccatc aatgccgcat agcccccata
    19861 actcccaccc acgatgcaga tgcgttcagg gtccgcgatg ccctgctcga tcgcgtagtg
    19921 tgcggcatcg gttaagtcgt cctgcatttc gagtccccac cgcttgaatc ccgcctgcaa
    19981 aaactccccg ccgtagccgc tggatcccct gaagttcact tgcagcacgg cccagccgcg
    20041 actggcgaga aattgggtcc agtaattgaa atccagcaca tctctgccgg ccggcccgcc
    20101 gtgaggcaac acaatcaacg gcccaggctg ctgagcatgc gccggacgcg tcacgtaccc
    20161 atgcaacacc gtgccgtctc tcgccttgag agtgagcggc atgggcttga ccaattcatt
    20221 cgggaccagc ttcggatacc cgtccaccat caaaatcagc cggttgagat cctcgtcgta
    20281 caggtaccat tgaggcggtt gcgtcgcatg gctggagacg acgatatgtc tgcggccgtt
    20341 ccgactgctt ccttgtatgt cattgattcg tccaggaagg gccagatcga ccctggtctg
    20401 cagtgctttc gccttaggat tccagtagat gctggcagat tcgtccaggc gactgcgaac
    20461 accgacaact tgtttgagcc aggacgaata gatgagaccg ccctcgatat cgtacaaggc
    20521 atgggatgcg cggagcgtag gcggcacctg cggattagaa atgtcgatgc tgtagactgc
    20581 agttcttcca tcgagacgtt gtgccacaaa gagcatgttg ggatcctcgt caaatcccag
    20641 aggagtcata tccggctcac gtagtgcatc atacttccgc aacgtctgcc agcgatcctg
    20701 gcccaccggt ttgaccagga tgtggaccat agtcccttca acggctgttc ccagccgcac
    20761 gacaccctgt cggtcggcca tccaatcccg catcccatag gtgcttccct cgatcaactc
    20821 ccggcggccg gtgtacacat ccaatttgta gacatcggga tacgtcccga tcctgagatc
    20881 gagtgcgatc aggacggatc ggttgtcccc gggaattttt ccgatgaccc tatcctgaag
    20941 ctgcggaaga tattctctgg taaaagaatt atggtcgcga ctcggagtga tcagatccgt
    21001 cttgagattc gagccgtcgc gattgatggc gaagaggcgc gtctccgtcc accggagatt
    21061 gctccaccca tcgtctgccc ccacgcccac caccaaccgt tcgtcattga cccagtcaaa
    21121 ccatcgaatc gagtaccgct tgttgtcgct ctccaagagt cttcgagcgt ccttgccgtc
    21181 gtaggtcgta accacgagat aggtcttgtc cccagcggta ttttgaatgg aggcgatgtg
    21241 ctcccccgac ggcgacagcg tcatcctacg taccagcggg agggacgcaa aagctttcag
    21301 gggaatacga ccgaggatgt cccaaaaact tctaccggcg gttgctcggg agtcggttgc
    21361 tcaaccgact tacagcctcc gatgaacgcc accgtcaaca ccaatatcgt caagatcgcg
    21421 atgagatgta cggaccggat tatcctcacg agaatcgccg tcatgcgctg acgggaaacc
    21481 atgatcccga tggaaaacga acaaccaggc atagcctacc ctcatcatat cgagagaaaa
    21541 ctctgagcac gaagcagaac caagtgtagg aagggactaa aaaacggtca acagaacgat
    21601 cgaatcctgc agcacacagg caggcaacga gctcatctcc catcgactcc aatctttccg
    21661 tgatgaaaat atctctagat ttcaatgagt taaattttta tttcatttac gggtgttgaa
    21721 cgtgcccgtt catacgtcat tagtaacgat gtgctacgcg ctggag
//
LOCUS       DAOQRM010000025        21107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1700_length_21107_cov_18.240880, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000025 DAOQRM010000000
VERSION     DAOQRM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21107
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1700_length_21107_cov_18.240880"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKK81_03860"
     CDS             <1..492
                     /locus_tag="PKK81_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32035.1"
                     /translation="MRYRSLVVLIGVLVTGLTGCSSHHHGSYGHGKGDYYWSKASQDV
                     NGLVEKHVKNPETAKQVNTVMGEMITEIKSAREQHRQSHRALYELNANYAATPEEFTK
                     ILDELNNNRMRSSARMLSLRFKMKSLLTAEEWSALSEELKRYGSRYYGKGAEAAAPAA
                     AQP"
     gene            complement(509..1117)
                     /locus_tag="PKK81_03865"
     CDS             complement(509..1117)
                     /locus_tag="PKK81_03865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNV32036.1"
                     /translation="MPIVLLIRHGETEWNRSGRVMGDQPIPLNRTGEEQARTCAGILS
                     RTPIAAIYTSPVLRAAQTAEILRGPQEIPLHQVPGLSEIGVGNWINRYWHEFADDPAK
                     REWYTHPDRARPSGGETLREVQQRAVAAVEQALAPAHDSPVVIVSHGDVIRAILAHYL
                     QLDLAIIRQARIDHVAVSGLDLTGTAAQLLFLNHRPGLETLL"
     gene            complement(1136..2167)
                     /locus_tag="PKK81_03870"
     CDS             complement(1136..2167)
                     /locus_tag="PKK81_03870"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32037.1"
                     /translation="MAALLRTSVCLVGLFLLACGSREAAVVSIALHPSNPNIVYVATN
                     EAVHKTRDGGATWEQFQAFTARRVTTLAIDPKLPATVYAGTMGDAIYKSPDGGQHWLP
                     HNVGLKEHVSFVNEIIFHPDNTELIYLATTVGAFVSKNGGREWEERMAGMKEVHIVTC
                     ITMDHQHPRVLYAGTTGGTYRSEDGGTTWQKKNNGLIPDDVLNAAMALGVNMLAVDPR
                     NSDIVYAATTKGLFRSTNRAGYWEQIGQNLANQFISTLALHPTDPKIVYIGGPEGILK
                     TVDGGKTWQAHNQGLTTMNVRTISLSPSDPQLLYLGTNGSGLYRSTNGGDQWTPIPLT
                     SHAQAAPAP"
     gene            complement(2249..3379)
                     /locus_tag="PKK81_03875"
     CDS             complement(2249..3379)
                     /locus_tag="PKK81_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HNV32038.1"
                     /translation="MRHSAFAALAVMVVLLTGTVHSVATDNQAPSFDTAKPGYRYEFP
                     KDHGAHERFRTEWWYYTGHLTAAGGRQFGFELTFFRRGIPPEQVRTQPSQWSIQQLYL
                     AHFALTDLDGNRFLFADKLSREGLGKAGAETDRLHVWLDRWSATMDDPTSSRQKLQAA
                     TDAFAIDLQVTPGKPPVLHGREGISRKGARPEQASHYYSFTHLTTEGEIRIGADTFSV
                     SGLSWMDHEFGSADLGRDVVGWDWFSLQLSDSTELMWYSLRRADGSADPVSSGTLVFA
                     DGRTQPLTAQDLTVESLDYWTSPRSKAQYPQQWRITAPSLGLNLDVRSLLADQELNTA
                     RSTRVMYWEGAVSATGQVHGTAVTGRGYVELTGYAERFTQRL"
     gene            3568..4257
                     /locus_tag="PKK81_03880"
     CDS             3568..4257
                     /locus_tag="PKK81_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNV32039.1"
                     /translation="MFLEPERDQSSRELSGKAQPFPVPERIPLFPLPNLVFFPKTYLP
                     LHVFEPRYRQMVADAAAGGQCIGMALLKEGWEEQYDGNPPIFSIGCVGRLASVQALPD
                     GRSNILLQGLERYEIHEEFYEKSYREARVALKPREGAVSMEPALRRYLTDVLDEYLKA
                     DEEASPLHSLVRPDVSDEVFVNSLSTYLDCTPLEKQFLLEADHVPQQARRLSDLIQFK
                     LAERHGAGGWG"
     gene            4257..5228
                     /locus_tag="PKK81_03885"
     CDS             4257..5228
                     /locus_tag="PKK81_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV32040.1"
                     /translation="MSRAVAIEVSHLGKTYEKVRAVDDLSFQVYAGEIFGLLGPNGAG
                     KSTTLRILITLLHPTSGSATILGMDSVKDADRVRKTIGYVPQERAIDRFLTGREHLEL
                     LADLYHLSPEDASTRIPQLLKLVELEQQADRPAKTYSGGMKRKLDIACGLLPDPKILF
                     LDEPTLGLDVQSRLRIWDHVRAMRERGITVVMTTNYLDEADQLCDRIAIIDGGRIKAL
                     GVPNELKAGLGGDLVSLTVREHDRVELLAAAVKNLPAIRAVTTTPTGLDIRVDSPEKA
                     LPAILEAANRLTCQLEFIDYHRPRLDDVFIAHTGRSIKDDQPKAEGQ"
     gene            5258..6037
                     /locus_tag="PKK81_03890"
     CDS             5258..6037
                     /locus_tag="PKK81_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV32041.1"
                     /translation="MKEYWQEIRALTMRWVRRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADMQVTQASSYISFLTAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMSTPIHR
                     TSVILSRFIFVTTITSLQVLVILGVAWLFGVQPVTGLLGVATILFIGMMFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLAAMPGWMGFLAQFNPMTWAIDAVR
                     PLILQGWAEALPHVGMVIGVMVLFDALCLYGGARAFRRAIG"
     gene            6064..6924
                     /locus_tag="PKK81_03895"
     CDS             6064..6924
                     /locus_tag="PKK81_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNV32042.1"
                     /translation="MFVNESQIRALIRLLGDEDERIVKTISGKLIDFGDSAVPLLQEA
                     EIEQPEMADRIVTVLEEIRGTRLEEELRDLIALPEDHIDLETGAFLLARYAYPTLDVQ
                     SYQRQLDRMAQDVREQIGSRVSGEETVKALNRYLFTEAGFRGNTKNYYEVENSYLNCV
                     MDRRTGIPISLSTVYLLIGKRLHLPVQGIGMPGHFLVKFDSDRYKIFIDCFNGGALLT
                     EKNCARFLTEAGYGFEDRFLQKSPTRAILARMIKNLLAIYNKLDEPLKKTRLPRFIEI
                     LGCEQREEGL"
     gene            complement(7114..7872)
                     /locus_tag="PKK81_03900"
     CDS             complement(7114..7872)
                     /locus_tag="PKK81_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV32043.1"
                     /translation="MIRVTVLGSGTNVHPTRAAAGYLVQTDQTFLLDFGPRTLSNLIT
                     SGVDRHRITHILFSHFHADHFADFIPFFFDAVIFCKYQGGTRPPLTLIGPHGTAKLMR
                     AMMTTFPSFNRAPFRVTIREVSDRSFRLGETRIKPGTVTHAPRLHCVGYRIEYQDHVV
                     TYSGDSLYCDNLVRLCHEADLAILDCSFPENRPGAGHLHAGLCGRVAQEAGINQLVLS
                     HFYPIAERYDVCAQAGNYFSGRIRMARDLLKLKA"
     gene            complement(7869..8327)
                     /locus_tag="PKK81_03905"
     CDS             complement(7869..8327)
                     /locus_tag="PKK81_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNV32044.1"
                     /translation="MELPDLKDDPHLKVLRPLVETYLAFWRMDSRHVRSLKLTPSQFD
                     VIATLGDTKGLTCAELSTATLVTKGTLTGVLDRLEDKGLIRRTPVADDRRSTRICLTE
                     KGDRLFKKTFAAHIAFLRPFFERALTTAEAEQLRTLLRRLHRSFQEKPTA"
     assembly_gap    8488..8587
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8588..8851
                     /locus_tag="PKK81_03910"
     CDS             <8588..8851
                     /locus_tag="PKK81_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV32045.1"
                     /translation="LKRALGDKQHQVRGMAASHLGVIGDDTAVAHLLAALEDDHAYVR
                     SSAALGLGRLRASAAKDKLAYVSKEDWDQTVRSRAREALERIR"
     gene            complement(8889..9722)
                     /locus_tag="PKK81_03915"
     CDS             complement(8889..9722)
                     /locus_tag="PKK81_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV32046.1"
                     /translation="MISSLMRWQELTRVWIGHWLSRPFHHLFNVMPGIEIGLSAPAIT
                     RLSLIHAPLAGRRAVHLSDLHLDRYRPRHRALVRTVADLRPDWIFITGDLLNVRDGLP
                     HVLRFLTELRRLAPVFVTLGNHDHYSGIRVDEFTEQFDRRKVTLLLNQVTFLPMDTGE
                     LAIVGLDDPSLHRADLRCIPSPRAGRFTLVLAHAPNILDQLAPEHHVDLTLCGHSHAG
                     QWRIPSIPTFWLPPGCHGRTNGMYTKGTHRLYVNRGIGWSVIPMRLNCPPEIVLLEWA
                     A"
     gene            complement(9719..10384)
                     /locus_tag="PKK81_03920"
     CDS             complement(9719..10384)
                     /locus_tag="PKK81_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32047.1"
                     /translation="MSIRFGHICCAVWLLCCAMTAGFAPSSMAATRLNGIDVFAQFTQ
                     HIRRQADTDKQAFAVASQCMTWFYKQQRRKPDPPAVQNIAWNRQTTSPWIFADSSEQE
                     GSDCRTRYPGGIDAVRSDFQRTQAFLSLSLTFYEFALVGDGDDNSTYSSRELHDMLMA
                     LNLAAEPSLGADAHLRSLTSHFDSLHEARGMEALMTGMGKLYDQGYRVTTADKAHLNR
                     VME"
     gene            10506..11558
                     /locus_tag="PKK81_03925"
     CDS             10506..11558
                     /locus_tag="PKK81_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNV32048.1"
                     /translation="MPPPSWLDDTVPYLTASVPALGGRIRSTPDDFCVEERPLYLPCG
                     EGEHLYIRVKKRGLSTPDLVLRLASQLHVKAQTIGVAGLKDAQAVTTQMLSLQGVKAE
                     TISALPTDERLLALEVLGRHRNRLRKGHHAGNQFRLVIRDVRERSEDDLQELFDELVR
                     RGVPNYFGPQRQGRSGTNFQLGAELLQDAARRNKMPRNKRIWFMNAYQSHVFNRIVAK
                     RIESIDRVFLGDWAMKSDNGACFPVEQPEVEQPRADRFEISPTGPLFGSRAPWATGVP
                     GDIEQAVVAELGTTPELLSKAGAECGFRGERRALRVRLNELSWSLEGTVLTLGFWLPP
                     GSYATSVLREVVKKSD"
     gene            11666..12910
                     /locus_tag="PKK81_03930"
     CDS             11666..12910
                     /locus_tag="PKK81_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HNV32049.1"
                     /translation="MTASQETVFLQGHIIDSLILAKVLDTILMMGGTFDLQDVKIGAT
                     RDASSQARIVVRAPSGRLLAEILRAIQPHGAAIEREADCRFEPAPAPGVFPEQFYATT
                     HLPTQIRLDGEWVEVEGMEMDLGICVDPAKARARTVPMGAVTAGDLIVTGREGIRVTP
                     LARPVERDVFGFMESVVSSERPHHPVIADIAQRMQKLREWHRQGRAGAKVLFAGGPAI
                     VHAGGREALAWLIESGYIQVLFCGNALAAHDMEAALYGTSLGYGLTAGRSVPHGHEHH
                     LRTINRIRTIGSIEQAVRSGVITGGIMAASVRCDVKLVMAGTIRDDGPLPGVLTDSMA
                     AQQAMRAEIPGVGLALLVASTLHAIATGNLLPATVPTVCVDVNPAVPTKLADRGSFQA
                     VGLVMDAASFLTELARELGWRP"
     gene            12907..>13658
                     /locus_tag="PKK81_03935"
     CDS             12907..>13658
                     /locus_tag="PKK81_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32050.1"
                     /translation="MSRLLVCPPDYFQIDYEINPWMRRENGVDPARAVRQWQALMTVL
                     EQDVGVGVERMQPVQGLPDLVFTANAGVVVGRRALVSRFRYPERQREEAHFARWFGER
                     GYEVLTLDPSLYFEGAGDLLGFPDTWFGGYRQRSDIRAFPKLSEIFQREIIPLELIDS
                     RFYHLDTCFCPLSGGDLLYLPSAFDSYGQAAIAERVPEDRRLAVPQEEALRFACNAVC
                     IGKQVVIPAGCPLTTKLLEDRGYRAHPVQLDE"
     assembly_gap    13659..13758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13759..14114)
                     /locus_tag="PKK81_03940"
     CDS             complement(<13759..14114)
                     /locus_tag="PKK81_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32051.1"
                     /translation="MKPCPACGRGLPEINRYCTHCGAGVSPDTSRRTAATPPGPAKEQ
                     LNLNILYGMVTVLIVSLLMPPWETPPSQPAAFLGFHFILSPPQPDALVSRLLLTIELT
                     TTAIAGLYLSFLFRTR"
     gene            14296..15321
                     /locus_tag="PKK81_03945"
     CDS             14296..15321
                     /locus_tag="PKK81_03945"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNV32052.1"
                     /translation="MEQPAIKHVAVIGAGAWGTALARHLAEKQVPVCLWAHEPEVVQA
                     IQLRRENTVYLPGVLLPPTLSATNVLSDAIAGADCLIFAVPSHVARTVLSRVAVLLPQ
                     PIPLVSATKGIEEETLALVTQVMQSVLPSHMQGSLLVLSGPSFASEVSRGKPTALCLA
                     GQDAGVVKAIQSLFMTPNFRVYADDDLTGVQLGGALKNVMALAAGVVDGLELGHNARA
                     ALITRGLAEMIRLGVAMGADPRTFYGLSGVGDLILTCTGPLSRNHSVGVRLGKGERLE
                     GILASMQAVAEGVRTAKAALGLALRCGVDMPIVQEVNAVLFADKSCRQAVGDLMEREA
                     KGEKSPS"
     gene            15318..>16060
                     /gene="larB"
                     /locus_tag="PKK81_03950"
     CDS             15318..>16060
                     /gene="larB"
                     /locus_tag="PKK81_03950"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNV32053.1"
                     /translation="MNQDQLQALLTKVQQGALAVPDALHRLRTLPYENLGFASLDHHR
                     ALRQGFPEVIFCEGKTVSQVVAIAKTLLRKNNALLATRVEPSVARALLRVSKRAVYHE
                     AARVVAIAPPKLVRRGAVLIVTAGTADIPVAEEARVTADIMGSKTDTLYDVGVAGLHR
                     LLGQQERLHDARALIVVAGMDGVLPSVVGGLVRQPVIAVPTSRGYGAHFGGLAALLTM
                     LNSCAAGVGVMNIDNGFGAGCLAHRINMVG"
     assembly_gap    16061..16160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16482..17054)
                     /locus_tag="PKK81_03955"
     CDS             complement(16482..17054)
                     /locus_tag="PKK81_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32054.1"
                     /translation="MINATPFSSTIVLRALRAVRLGLLFLLGAALLLGGHPMDASAKT
                     KGKSSVPRPEPDLKILSLHAAPMPYRPQDGPFHFIATVQLPKEVDEQLMLEVSALVTS
                     SSMTSLRFLSIRQPVNEHAQTATSAAGDTPQKHVQVDLVWDGLDHNKQQVPAGSYEYE
                     VRVKLLSNGDKGQRTQMLAWPKRGKVVVKE"
     gene            17224..18432
                     /gene="larC"
                     /locus_tag="PKK81_03960"
     CDS             17224..18432
                     /gene="larC"
                     /locus_tag="PKK81_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNV32055.1"
                     /translation="MATHLHFDCFSGISGDMTLGALVDAGLPFKDLVRGLALLRIEGF
                     RLTRKRVERGALTATKVDVLVEKGFRAPLTLAQIKRILLKSGLPPVVKEKSQAVFDVL
                     AHAEGKAHGVEPSHVHFHEVGVIDSFVDVVGGVLGLHLLGVQRVTASAINVGSGTLMS
                     AHGSLPVPGPAVAALAVGLPIYAGGPERELATPTGVALVRTLATEFGRLPHMQVRRVG
                     YGAGTADPAHWPNVLRVFVGEEPAAAGSVETIVELQTNIDDLNPQVYETVFDRVFAAG
                     AVDATLAPVTMKKGRPGNVLSVLAPQEKVEAVLAVLFADTTALGVRTHEVQRRVLPRR
                     FVPVQVHGHEVSIKVADSQPGRSKAAPEYEDCKRIAEQSGRPVKDILEEAMQAYRRTA
                     GHAKKEKGSR"
     gene            18491..19066
                     /locus_tag="PKK81_03965"
     CDS             18491..19066
                     /locus_tag="PKK81_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32056.1"
                     /translation="MTILLYVGLFVASVVVTLAGCHLFTNAIEWLGKRLNISEGAVGS
                     VFAAVGTTLPETSIPIIAIFFGAGREQMEVGLGAILGAPFMLSTLVLPILALLLLLYA
                     KLGKRTATFKLNYGEVRVDISFFLISYALALTCAVIPSKLFHVVVAAVLLGMYVYYMK
                     LKFSAEDEEGEGGGELSHCCSHAGPLVRRIW"
     gene            19084..19503
                     /locus_tag="PKK81_03970"
     CDS             19084..19503
                     /locus_tag="PKK81_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32057.1"
                     /translation="MGLLGLVGGAHLFVTAANSISAEMQVSPLILALLIAPLATELPE
                     MSNSFLWLYRKKDRLAVGNVTGAMVFQGSIPVSVGLLGTEWTLGTTALATMVLAVLAM
                     SLSLLQAVWSGHWRPWLLSGSALLYLGYTLFLYAHGS"
     gene            19544..20536
                     /gene="pdxA"
                     /locus_tag="PKK81_03975"
     CDS             19544..20536
                     /gene="pdxA"
                     /locus_tag="PKK81_03975"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNV32058.1"
                     /translation="MGDPAGIGPEVIAKALADRALTRLCTPVVVGSRQVMERTIAWLK
                     LPLQVVDFNPANGQRLKAGQVAVADPLESPLPKFRMGAATAVTGAASIAFIKEAVKLA
                     QAGSLGGIVTAPINKEAMNMAGFHYPGHTELLADLTKTTEFGMMIVGGPLKIMFTTTH
                     VAINALSPLLTTERISKAIRLAHLGLTKYFGIARPRIGVAALNPHAGEHGLFGNEEST
                     SIAPAVKLARAAGIKASDPLPADTLFGKAARGDYDGVVAMYHDQGLIPLKLVAFGTCV
                     NLTVGLPIIRTSVDHGTAYDIAGKGVAEHGSLLEAVKVAARLAESWSPVTKTSR"
     gene            20557..20877
                     /locus_tag="PKK81_03980"
     CDS             20557..20877
                     /locus_tag="PKK81_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32059.1"
                     /translation="MAETGLSAATMIDQQVKELDAIATRTIQDFNTTLGTERMGQWKI
                     RTVALLQQHAGQNAADELARKKPGMAFTNDLIEEFTDEVESYRSYLVTLAKRIRAAAP
                     PAAG"
     gene            20894..>21107
                     /locus_tag="PKK81_03985"
     CDS             20894..>21107
                     /locus_tag="PKK81_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNV32060.1"
                     /translation="MASSLPPALKRLGQNFLIDPNIIRKIVSLAALGPEDTVLEIGPG
                     RGALTAGLCAEAGRVIAVEIDPQLQPH"
BASE COUNT         3888 a         5802 c         6833 g         4284 t
ORIGIN      
        1 atgcgttatc gatccttggt tgtcctcatc ggcgtgcttg tgaccggatt gaccggttgt
       61 tcatcccatc accacgggag ttatggacat gggaagggcg actattattg gagcaaggct
      121 tctcaggatg tgaacggctt ggttgaaaag catgtcaaaa atccggagac cgcgaagcag
      181 gtcaatacgg tgatgggaga gatgatcacg gagatcaagt cggcacggga gcagcatcgc
      241 cagtctcatc gcgcgctcta cgagctgaac gccaactatg cggcgacgcc ggaggagttt
      301 acgaaaattc tggacgagtt gaacaacaac cggatgcgtt cgtccgcacg gatgctcagc
      361 cttcggttca agatgaagtc cttgctgaca gccgaggaat ggtcggcatt gtcggaggaa
      421 ttgaaacggt atggcagccg gtactacggc aagggcgcag aagctgcggc ccctgctgcc
      481 gcccaaccct agaaggtagg gtggtcgact acagaagcgt ttctaagccg gggcgatggt
      541 tgaggaacag cagctgggct gcggtgcctg tcaggtccaa cccgctgacc gccacgtgat
      601 cgatgcgagc ctggcggatg atcgccaggt cgagctgcag gtagtgcgcg agaatcgccc
      661 gaatgacatc cccgtgggac acgatcacga cggggctgtc gtgggcgggc gcgagggctt
      721 gttccactgc cgccaccgcc cgctgttgca cctcgcgcag cgtttctccg ccggaaggcc
      781 gggcccggtc cgggtgggtg taccactccc gcttggccgg gtcgtcggcg aattcatgcc
      841 aatagcgatt gatccaattc cccacgccga tctcactcag cccgggaacc tgatgtagcg
      901 ggatctcctg tggcccacgt agaatctcgg cggtctgcgc ggcccgcagg accgggctgg
      961 tgtagatggc cgcgatcggg gtgcgagaca ggatgccggc gcaggtgcgg gcctgttctt
     1021 cgccggtccg gttgagggga atcggttgat cgcccatcac ccggccggat cgattccact
     1081 ccgtttctcc atgtctgatg agcaggacga tcggcatcgg gagctctcgc caatcctacg
     1141 gcgcgggcgc cgcttgcgcg tgactggtca ggggaatcgg cgtccactgg tccccgccgt
     1201 tggtcgaccg atacaacccg ctaccattgg tccccagata gagcagctga ggatcgctcg
     1261 ggctaaggct gatcgtccgc acattcatgg tcgtcagtcc ctggttgtgg gcttgccagg
     1321 ttttcccgcc atcgacggtc ttgagaatac cctccgggcc accgatgtac acaatcttgg
     1381 gatcggtcgg atgcagggcc agggtgctga tgaattgatt ggccaggttc tggccgatct
     1441 gttcccagta gccggcccgg ttggtggatc gaaaaagccc cttggtggtg gcggcgtaga
     1501 cgatgtcgga attacgcgga tcgaccgcca gcatattcac cccgagcgcc atggcggcgt
     1561 tcagcacatc gtcaggtatg agtccgttgt ttttcttctg ccaggtcgtg ccgccgtctt
     1621 cactgcgata ggtgccgcct gtcgtcccgg cgtacaagac gcgagggtgt tgatgatcca
     1681 tggtgatgca ggtgacgata tgcacttcct tcatgcccgc catccgctct tcccattccc
     1741 ggcctccgtt tttgctgaca aaggcgccga ccgtcgtggc aaggtagatg agctcggtat
     1801 tgtcgggatg gaagatgatt tcattgacga aggacacatg ctctttgagc ccgacattgt
     1861 gcgggagcca atgctgcccg ccgtccgggc tcttgtagat ggcatcgccc attgtgcccg
     1921 cgtagaccgt ggcagggagt ttcgggtcga tcgccagggt cgtcacgcgg cgggccgtga
     1981 acgcctgaaa ctgttcccag gtcgcgccac cgtcgcgggt cttgtgcacg gcttcgttcg
     2041 tcgcgacata gacgatgttg gggttcgacg gatggagcgc gatcgagacc accgccgctt
     2101 cgcgcgaccc gcaggccagg aggaacaagc cgaccaggca gacggatgtc cggagcaggg
     2161 cagccattcg agttcgtcgt gtcatcacaa tggccgcaga cctaaccata gcgtggcgat
     2221 tgagtcaagg tagacatcag gcgagtggct acagtcgctg ggtgaagcgc tcggcgtatc
     2281 ccgtgagttc gacatagcca cgtcccgtga cggctgtgcc gtggacctgt ccggtggcgg
     2341 atacggcccc ttcccagtac atcacgcggg tgctgcgtgc ggtattcaat tcctggtcgg
     2401 cgagcagcga tcgtacatcc agattgagtc ccaggctggg cgccgtgatt cgccactgtt
     2461 gcggatactg cgccttcgat cgcgggcttg tccaatagtc gagcgactca acggtgaggt
     2521 cctgggccgt gagcggctga gttcgtccat cggcaaagac caaggtgccg ctggagacgg
     2581 ggtccgccga tccatcggcc cgccgcaggg aataccacat cagttcggtc gagtcgctca
     2641 gctgcaggct gaaccagtcc catcccacca catcccgtcc caggtcggct gagccgaatt
     2701 catgatccat ccagctgaga ccggagacgg aaaaggtgtc cgcgccgatg cgaatctccc
     2761 cctcggttgt caggtgggtg aacgaataat agtgagaggc ctgctcaggt cgcgcgccct
     2821 tgcggctgat gccctcccgg ccgtggagca cgggcggctt ccccggggtg acctgcaaat
     2881 cgatcgcaaa tgcatcggtg gcggcctgga gcttctgtcg ggacgacgtg ggatcatcca
     2941 tggtggctga ccagcgatcg agccagacat gcagtcggtc cgtctccgcg ccggcctttc
     3001 cgagaccttc gcgactgagt ttgtcggcaa aaaggaatcg gttgccgtcg aggtcggtga
     3061 gggcgaagtg ggccagatag agttgttgaa tcgaccactg cgagggttga gtccggactt
     3121 gttccggtgg aatgcctcgc ctgaaaaagg tgagctcaaa tccgaactgc cggcctccgg
     3181 cagccgtgag atggccggtg taataccacc attcagtacg aaaccgttcg tgggcgccat
     3241 ggtctttagg aaattcatac cggtagccgg gcttggcggt gtcaaatgac ggtgcctggt
     3301 tgtccgttgc cacagaatga acggtgccgg tcagaagcac gaccataacc gccagggcgg
     3361 cgaatgcaga atggcgcact gtgatcacgt ccgactcacc ggctgtcgcc tgtgcgcggt
     3421 gaggagtggc ggcctatgat gcggtgccat attttgatga gggaccgacg gaacgccagt
     3481 attcatgagg gttttgcgag ggtcatttcc gtgactgcct tgcccggcag cgttgacaat
     3541 tttgcaaagc atcagctatc gtgagccatg tttcttgagc ccgaacgtga ccagtcgagc
     3601 cgggagttat cgggaaaggc gcagccgttt ccggtgccgg agcgcattcc gctgtttccc
     3661 cttcccaacc tcgtcttctt tcccaaaacc tatctgccgc tccatgtgtt cgaaccgcgc
     3721 taccgccaga tggtagcgga tgcggcggcg ggagggcaat gcatcgggat ggcgttgctt
     3781 aaagaaggat gggaagaaca gtacgacgga aatcccccga tcttttcgat cgggtgtgtc
     3841 ggacggttgg ccagtgttca agccttgccc gatggccgct cgaacatcct cctccaaggc
     3901 cttgagcgct atgaaataca cgaagagttt tatgagaaga gttatcgcga agcccgggtc
     3961 gcgctgaagc cgcgggaagg cgctgtctcc atggagccgg cgctacgccg ctatctgacg
     4021 gacgtgctcg acgaatatct caaggccgat gaggaagcct cgccgctgca cagcctggtc
     4081 cggcccgatg tgagcgacga ggtgttcgtg aattccctct cgacgtatct cgactgcacc
     4141 ccgctggaga agcagttttt gctggaagcg gaccatgtgc cgcagcaggc ccgccgtctc
     4201 agcgacctca tccagttcaa gctggcggag cggcacggtg ccggagggtg gggctgatgt
     4261 cgcgggcggt cgccatcgag gtgtcgcatc tcgggaaaac gtacgagaag gtccgtgccg
     4321 ttgatgacct gtcctttcag gtctatgcgg gagagatatt cggtctgctc ggcccgaacg
     4381 gcgccggtaa gagtaccaca ctccgtatcc tgatcacgct gctccatccc acatccgggt
     4441 cggcgacgat tctcggcatg gattcggtga aggatgccga ccgcgtcagg aagacgatcg
     4501 ggtacgtgcc tcaggagcgg gcgatcgatc ggttcctgac ggggcgggaa catctcgagc
     4561 tactcgccga tctctatcac ctgtcgcctg aagacgcgtc cacacgtatc cctcaattat
     4621 tgaagctggt tgaattagag cagcaggccg atcgtcccgc caagacctat tccggtggca
     4681 tgaaacggaa attggatatc gcctgcggtc tcttgcccga tccgaagatc ctctttctcg
     4741 acgagccgac gctcggcttg gatgtacaaa gccggttgcg catttgggat catgtccggg
     4801 ccatgcggga gcggggcatt accgtcgtca tgaccaccaa ctatctggat gaggccgatc
     4861 agctctgtga tcggatcgct atcattgacg gaggtcggat caaggccctg ggcgttccca
     4921 acgagttgaa agccggtttg ggcggagact tggtgtcgct gacggtgcgg gagcatgatc
     4981 gtgttgagtt gcttgcggcg gcggtgaaaa atctgccggc cattcgagct gtgacgacca
     5041 ccccgaccgg gctggatatt cgagtcgatt ctcccgagaa ggccctgccg gccattcttg
     5101 aagcggcgaa tcgtctgacc tgtcagctgg agttcatcga ctatcaccgg ccgcgattgg
     5161 atgatgtgtt tattgcccat acggggcggt ctatcaaaga cgaccaaccg aaggcagagg
     5221 gtcagtaagg ctcgatcaag agcgttgtga agggtctatg aaagaatatt ggcaggagat
     5281 tcgggcactg acgatgcgct gggtgcgccg gctgagccgg gaaaagttca gcatgctctt
     5341 taccctggtc cagccgatgt tattctggtt gatcttcttc gggaatttgt ttcaacgcgc
     5401 cgcagacatg caggtgacgc aagcgtcgag ctacatcagc tttctgaccg ccggtgtggt
     5461 cgttatgacg gtgttgaaca acggattggc cggtggcgtg gatctgctct tcgacaagga
     5521 gaacgggttt ctcgaacggc tgatgtccac cccgattcac cgcacgtcgg tgattctgag
     5581 ccggttcatt tttgtgacga ccatcacgtc gttgcaagtg ctggtcattc ttggtgtggc
     5641 ctggttgttc ggggtgcagc cggtcacggg attgttgggg gtggcgacga tcttgttcat
     5701 cggcatgatg ttcggtgtcg ggctgacggc gatttccatg gccatggcct tttcagtgaa
     5761 aagccacggc gattttttct cggtcctcgg gtttctctcc ctgcccatga ttttcttgag
     5821 ctcggccctg gtgccgctgg cggcgatgcc gggctggatg ggttttctcg cgcagtttaa
     5881 tcccatgacc tgggcgattg atgccgtgcg gccgctcatt ctgcagggat gggctgaagc
     5941 cctgccccat gtcggcatgg tcatcggggt gatggtgttg ttcgatgcgc tgtgcctcta
     6001 tggcggcgcg cgcgctttcc ggcgggcgat cgggtgaatg ggattgcgca gcggagtcct
     6061 tccatgtttg tgaatgaaag ccaaatccgc gccttgattc gactcctcgg cgatgaggac
     6121 gaacggatcg tcaagacgat cagcgggaag ctcatcgatt tcggcgactc cgccgtgccg
     6181 ctcctgcagg aagcggagat cgagcagccg gagatggccg atcgtatcgt cacggtgctg
     6241 gaggaaatcc gcgggaccag gctggaggaa gaactccggg atctcatcgc ccttccggaa
     6301 gatcacatcg atcttgaaac gggggcgttc ctgttggcgc ggtatgccta tccgacgctt
     6361 gacgtccaga gctatcagcg acaattggat cgtatggctc aggatgtgcg ggagcagatc
     6421 gggtcccggg tctcagggga agagactgtc aaggcgctca atcgctacct gttcaccgaa
     6481 gcggggttta gaggaaacac caagaactac tatgaggtcg agaacagtta tctcaactgt
     6541 gtgatggacc ggcgaacagg aatcccgatc agtctttcaa ccgtgtatct gctcattgga
     6601 aagcggttgc acctgccggt gcaggggatc ggcatgccgg gccacttcct cgtcaagttc
     6661 gactccgatc ggtataagat cttcatcgat tgtttcaacg ggggcgcact gctgacggaa
     6721 aaaaactgcg cccgtttcct gacggaagcc ggttacggat tcgaagaccg gtttctccag
     6781 aagagtccca ctcgcgcgat tctcgcccgc atgatcaaga atctgctcgc catctacaat
     6841 aagctcgatg agccgctgaa aaagacccgc ctgcctcgct tcattgaaat attggggtgt
     6901 gagcaacgag aagaagggct gtagcaggat gctgaaaaag tccgtcagct tcgttctcgc
     6961 ttcgttcaga tcctcaacgt accgcaaggg tacgcctcgg atcttcactc gctgcggcct
     7021 tgctggacgg gctttttgag catcctgctc gtctgttctc gccagcgtgc ctctctcttt
     7081 tatgttctag aacgaaccac cgacggttcc tagctaagct ttgagcttga gcaggtcccg
     7141 cgccatccga atgcgtcctg agaaatagtt tcccgcctgt gcgcagacgt cataccgttc
     7201 tgcaatcgga taaaaatggg acagtacaag ttggttgatg ccggcttcct gcgccacgcg
     7261 gccgcagagc ccggcgtgga gatgtcctgc gcccgggcgg ttttccggaa aggaacagtc
     7321 cagaatggca agatctgcct cgtggcagag acggaccagg ttgtcgcaat agagggaatc
     7381 tcccgagtag gtgacgacat ggtcctggta ttcgatgcgg tagcctacgc aatgcagtcg
     7441 aggcgcatgg gtcaccgtcc cgggtttgat tcgggtctcg ccgaggcgga agctgcgatc
     7501 ggagacctct cgaatcgtga cgcgaaacgg agcgcggttg aagctcggga atgtggtcat
     7561 catcgcgcgc atcaacttgg cggtgccatg cggaccgatc agggtcaacg gaggtctggt
     7621 gccgccttgg tacttgcaga agatgacggc atcgaagaag aagggaatga aatccgcaaa
     7681 gtggtcggcg tggaaatgag aaaacagaat atgtgtaatc cggtgccgat cgacaccgct
     7741 ggtgatcaag ttgctgagcg tgcggggtcc gaagtcgagg agaaaagtct ggtcggtctg
     7801 aaccaggtac ccggccgcag cgcgtgtcgg gtgaacgttt gtgcctgagc ccagcaccgt
     7861 gacccggatc atgccgtcgg cttctcctga aagctccggt ggaggcggcg gaggagcgtg
     7921 cgcaattgct ccgcttctgc ggtggtgagc gctcgttcga aaaaggggcg taaaaatgcg
     7981 atgtgggccg caaatgtttt cttgaacagg cggtcgcctt tctcggtcag gcagatgcgc
     8041 gtgcttcgtc ggtcatcggc gaccggcgtg cgtcgaatca gccccttatc ctccaggcgg
     8101 tccagcacgc cggtcagggt ccccttggtc accaaggtgg cggtagagag ttcggcgcag
     8161 gtcagtcctt tggtatcgcc caacgtggca atcacgtcga attgggaggg ggtcagcttc
     8221 aacgaacgca cgtgacggct atccatgcgc caaaaggcga ggtaggtctc gaccaacggg
     8281 cgcaggactt tgaggtgtgg atcgtctttg aggtcgggta actccattgc gcgcgcagcc
     8341 tagtcatccc atcgggtagc tgtcaagagt cggccgccca aggtcgcgtt gacgccgcac
     8401 agatcgtccc cctatcatgt cgcctgccat gcctcgtcac gcgcaatccg gccaagccaa
     8461 tctgaccacg attctcctcc tgctgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnctc aagcgggcgt tgggagacaa acaacatcag gtacggggga tggcggcaag
     8641 ccacctggga gtgatcggcg acgacacggc ggtggcccat ctcctggcgg ccttggaaga
     8701 tgaccatgcc tatgtgcgat ccagcgcggc attgggcctc ggtcggttgc gagccagcgc
     8761 ggccaaggac aagttggcct atgtcagcaa ggaagactgg gatcaaacgg tgcggagtcg
     8821 ggcacgcgag gcgttggaac gtattcgatg agaaggcagc gtagcgggca gagcagatgg
     8881 tcgaacgatt acgcggccca ttcgagcaga acgatttcag gcgggcaatt caaccgcatg
     8941 gggatgaccg accatccgat cccacggttc acgtagagcc ggtgggtgcc tttggtatac
     9001 atgccgtttg tccgtccatg gcagcccggc ggcaaccaga acgtgggaat ggaggggatc
     9061 cgccactgcc cggcatggct gtgtccgcag agggtcaagt ccacgtggtg ttcaggcgcg
     9121 agctggtcca ggatattcgg cgcatgcgcc agcacgagag tgaatcgtcc agcccgcggt
     9181 gagggaatgc aacggagatc tgcacggtgc agtgacggat cgtcaagccc gacgatggcc
     9241 agttcccctg tgtccatcgg caggaacgtg acttggttga gcaggagcgt gaccttgcgt
     9301 cggtcgaatt gctcggtaaa ttcgtccacc cggatgccgc tgtagtggtc gtggttaccg
     9361 agcgtgacga agaccggtgc cagacgccgg agttccgtca gaaagcgaag cacatgcggc
     9421 aggccgtcgc ggacgttcag gaggtcgccg gtgataaaga tccaatccgg cctcaggtcg
     9481 gcgacggtgc ggacgagcgc ccggtgtctg gggcgatagc ggtccagatg gaggtcgctg
     9541 agatgcacgg ctcgccggcc cgccagcgga gcatggatga gggagaggcg ggtgatggcc
     9601 ggcgcggaca gcccgatttc gatccccggc atcacattga acagatgatg aaaggggcgg
     9661 ctcagccaat ggccgatcca gacgcgcgtc agctcctgcc accgcatcaa ggaggagatc
     9721 attccatcac tcgattgaga tgcgccttgt ctgcggtggt gacgcggtag ccctggtcat
     9781 acagtttgcc catgccggtc atgagcgctt ccatgccccg ggcttcgtgt agggaatcga
     9841 agtgtgaagt gagggaacgg agatgggcat ctgctccaag cgatggttca gcggccaggt
     9901 tcagcgccat cagcatgtcg tgcagttcac ggctgctgta ggtcgagttg tcgtcgccgt
     9961 ccccgaccag ggcgaactcg taaaaggtca ggctcagcga gagaaacgcc tgggtgcgtt
    10021 ggaagtcgct gcgaacggcg tcgatcccgc ccgggtaacg cgtgcggcag tcggagcctt
    10081 cctgctctga agaatcagcg aagatccatg gtgacgttgt ctgtcggttc caggcaatgt
    10141 tctgcacggc aggcgggtcg ggtttgcgcc gctgctgctt gtagaaccag gtcatgcatt
    10201 gactggccac agcaaaggcc tgcttgtcgg tgtcagcctg gcggcggatg tgctgggtga
    10261 attgggcgaa gacatcgatg ccgttcaacc gggtcgcggc catggatgag ggggcgaagc
    10321 cggccgtcat cgcgcagcac agcagccaga cggcgcagca gatatgacca aatcggatcg
    10381 acatcgggtt cgtctacaga taattgcagg agtcgtggac ggcctcattc gagtataaca
    10441 cgggtcgtgc ctgtgcctac ccaaatgacc gcaattgtgc ccgatgaagc acgagaagga
    10501 aacctatgcc gcctccgtcc tggctcgatg acacggtgcc ctatttgacc gcctcggttc
    10561 ccgccctggg cggccggatc cgcagtactc cggatgattt ttgtgtggag gaacggccac
    10621 tgtatttgcc ttgcggcgaa ggtgagcatc tgtatatccg ggtaaaaaaa cgggggctgt
    10681 ccacgccgga tctcgtcttg cgacttgcct cccagctcca cgtgaaagcc cagaccatcg
    10741 gggtggccgg gctgaaagat gcgcaggccg tgacgacgca gatgttgtcg ttgcagggag
    10801 tgaaggcaga aaccatctcg gcattaccca ccgacgagcg gttgctggcg ctggaagtcc
    10861 tggggcgcca tcgcaaccgg cttcgtaagg ggcatcatgc gggcaaccag tttcgtctgg
    10921 tgatccggga tgttcgggag cgcagcgaag acgacctgca agagttgttc gatgaactcg
    10981 tgcggcgggg cgtccccaac tatttcggtc cgcaacggca agggcgatcg gggacgaact
    11041 ttcaattggg tgccgagttg ctccaggacg cggcccgtcg gaacaagatg cctcgcaaca
    11101 aacgcatctg gttcatgaat gcctaccagt cccatgtgtt caatcgcatc gtcgccaaac
    11161 ggatcgagag catcgaccgg gtgtttctgg gcgactgggc gatgaaatcg gacaacggtg
    11221 cctgttttcc tgtagagcag ccggaggtcg agcagccgcg tgccgaccgg tttgagatca
    11281 gtccgaccgg accactcttt ggctcccgcg caccctgggc gacgggggtc ccgggtgaca
    11341 ttgaacaggc cgtggtggct gagctgggca ccacgccgga gcttctgtcg aaagccggcg
    11401 ccgaatgtgg attccgtggc gaacggcgag ccctgcgcgt ccgtctcaac gagttgtcct
    11461 ggtcgctgga gggcactgtg ctgacccttg gcttctggct ccctcccggt tcctacgcca
    11521 cgagcgtcct gcgggaagtg gtgaagaaaa gcgactgacc ctcgcgcctc attccttccg
    11581 gcagatggta gaatttacag agtcaggcat gttccccccg gacgccttcc gctccggcgc
    11641 cggcacgagt cgtgagaggt ccgtcatgac cgcatcgcaa gaaaccgtgt tcctgcaggg
    11701 gcacattatc gattcgttga tcctggccaa agtcctggat acgattctga tgatgggcgg
    11761 tactttcgat ctgcaggacg tcaagatcgg cgcgaccagg gatgcctctt cccaggcgcg
    11821 gattgtggtg cgagccccgt ccggccgcct gttggccgaa attctgcggg ccatccaacc
    11881 tcacggtgcc gccattgagc gtgaagcgga ctgtcgcttc gagccggcac cggctccggg
    11941 agtgtttccc gaacagttct atgcgacgac acatttgccg acccagattc gtctggatgg
    12001 cgagtgggtg gaggtggagg gcatggaaat ggaccttggc atctgtgtcg atccggccaa
    12061 ggcccgcgcc aggaccgttc ccatgggggc ggtgacagcc ggcgacctca tcgtgaccgg
    12121 gcgggaggga atcagggtta cacccctcgc tcgtcctgtc gagcgtgacg tgttcggatt
    12181 tatggaatct gtggtgtcgt cggagcggcc gcatcaccct gtcattgccg acatcgctca
    12241 gcgcatgcag aagttgcgcg aatggcatcg acaggggcgc gccggggcca aggtgctctt
    12301 tgccggcggc ccagcgatcg tgcacgccgg ggggcgagag gccctggcct ggttgatcga
    12361 gtccgggtac atacaggtct tgttctgcgg caatgccttg gctgcgcacg atatggaagc
    12421 cgcactctac ggcacgtcgc tcgggtatgg gctgacggcc ggccgttcgg tgccgcacgg
    12481 gcatgagcat catctgcgca ccatcaatcg cattcgtacc atcggcagca tcgagcaggc
    12541 tgtgcggtcg ggcgtcatca ccggcggaat catggcggcc tctgtgcggt gcgatgtgaa
    12601 gctggtgatg gcgggcacca ttcgcgacga cggtcccttg cccggggtgc tgacggactc
    12661 catggcggcg cagcaggcca tgcgcgcgga aattccaggg gtcggacttg ccttactggt
    12721 tgcatccacg ctgcatgcga tcgcgaccgg caatttgttg ccggccaccg tgcccacggt
    12781 gtgcgtcgat gtgaatccgg cggtgccgac gaaattggct gaccgtggca gtttccaagc
    12841 cgtcggcctt gtcatggatg ccgcctcctt tctcacggaa ctcgcgcgtg aactcgggtg
    12901 gcgtccatga gccgcctgct cgtgtgcccc cccgattatt ttcagatcga ttacgagatc
    12961 aatccctgga tgcggcggga aaacggtgtg gatccagcgc gtgccgtccg gcaatggcag
    13021 gcgctgatga ccgtgctgga gcaggatgtc ggtgtcgggg ttgagcggat gcagcctgtt
    13081 caggggctgc ccgacctggt ctttacggcc aatgcgggag tcgtcgtcgg tcgtcgcgcg
    13141 ctggtgagcc gctttcgcta tccggagcga cagcgggagg aagcgcattt tgcgcgatgg
    13201 ttcggtgaac gcggatatga agtattgacg ctcgacccgt ccctgtattt cgaaggcgcc
    13261 ggcgatctgc tgggatttcc ggacacctgg ttcggcggat accgccagcg gtcggatatc
    13321 cgggcgtttc cgaagttaag cgaaatcttc cagcgggaaa tcattcctct cgaattgatc
    13381 gacagccgct tctatcacct cgacacctgt ttttgtccgc tcagcggcgg cgacttgctc
    13441 tacctgccct cggcgttcga ctcctacggc caggcggcca tcgcggaacg ggtcccggag
    13501 gatcgtcggc tcgcggtgcc tcaagaagaa gcgcttagat ttgcctgcaa tgcggtctgt
    13561 atcggcaaac aggtcgtgat ccccgccggt tgtccgctca cgacgaagct cctggaagat
    13621 cgaggctatc gcgcccatcc cgttcagttg gacgaattnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnntg tctcgtgcgg aatagaaacg acagatacag gccggcgatg
    13801 gcggtggtgg tgagctcaat cgtgagcagc agccggctga cgagggcatc cggttgcggc
    13861 ggggagagga tgaaatgaaa cccgagaaag gccgccggct gtgagggggg cgtttcccag
    13921 ggcggcatga gtagggagac aatcaggacg gtcaccatgc cgtacaggat attcaggttc
    13981 agctgttcct tggccggtcc gggaggagtc gccgccgttc tccggctggt gtcagggcta
    14041 acacctgctc cgcagtgggt gcaatagcgg ttgatttccg gtaggccacg tccgcaggca
    14101 ggacagggtt tcatgacagt cgttccagat gaagtctgca gcctaacgcg acggcggcag
    14161 gatttccagt ggcgagatag ggcatcgctt cgttgacagc tttcaaagcc ccttcctata
    14221 atccgcgtcc atgaagtctg cattctgcat tccagctgcc ggcccgctct gccacgtcat
    14281 ggtggtgggg cagtgatgga gcaaccggcc atcaagcatg tggcagtcat cggcgccggg
    14341 gcctggggga cggcgttggc ccggcatctg gccgagaaac aggtgcccgt ctgtttgtgg
    14401 gcccatgagc cggaagtcgt tcaggcgatc cagctcaggc gtgaaaatac ggtgtacctt
    14461 ccgggcgtgt tgcttccgcc gaccctctcg gcaacgaatg tcttatccga cgccatcgca
    14521 ggcgctgatt gcctgatttt tgcggtgccc tcgcatgtag cgagaacggt gctgagccgg
    14581 gttgcagtct tattgccgca gcccattccg ctggtcagtg cgaccaaggg gatcgaagaa
    14641 gagacgcttg cgctcgtgac gcaggtcatg cagagcgtgt tgccctcgca catgcagggt
    14701 tccttgttgg tcctgtcggg accgagtttt gccagtgagg tcagccgcgg gaaaccgacg
    14761 gcgctgtgtc tcgccgggca ggatgccggt gtcgtcaagg cgatccagtc gctgttcatg
    14821 acgccgaact ttcgcgtcta tgcggacgat gacttgaccg gtgtgcaact cggcggcgcc
    14881 ttgaaaaacg tgatggccct ggctgccggt gtcgtggatg ggttggaact cgggcataac
    14941 gcccgcgccg cgctcattac ccgcggcctg gccgaaatga tccggctggg tgtggcgatg
    15001 ggtgctgatc cacggacttt ctatggcctc tcaggggtcg gtgatctcat tctgacctgc
    15061 accggtccct tgagccgcaa tcattcagtc ggggtgcggc tggggaaagg cgaacgcttg
    15121 gagggaattc tcgcgagcat gcaggcggtg gcggaaggcg tgcgcacggc caaggcggca
    15181 ttggggctcg ccctgcgctg tggggtggat atgccgatcg tgcaggaagt aaacgcggtg
    15241 ctctttgccg acaagtcctg tcgtcaggcg gtcggtgatc tcatggagcg cgaagccaag
    15301 ggggagaaga gcccgtcatg aatcaggacc agctccaggc gttgctcacg aaggttcagc
    15361 agggcgctct tgcggtgccg gatgcgcttc accggttacg caccctgccg tatgaaaacc
    15421 tgggatttgc ctcgctcgat caccatcgcg cgctgcgcca ggggtttccg gaagtcattt
    15481 tttgcgaagg caagacggta tcacaggtgg ttgccatcgc gaagacgctc ttgaggaaga
    15541 acaacgcctt gctggcgacg cgggtggaac cctcggttgc gcgggcgctc ctgcgcgtga
    15601 gcaagcgggc ggtctaccac gaagcggcgc gggtcgtcgc cattgcgccg cccaagttgg
    15661 tgcgccgggg ggccgtattg atcgtcaccg ccggcaccgc cgatattccc gtggcggaag
    15721 aagcgcgtgt gacggccgat atcatgggga gcaagaccga cacgttgtac gatgtgggag
    15781 tggccggctt gcatcgcctg ctggggcagc aggaacggtt gcatgatgcc cgggccctca
    15841 tcgtggtggc cggcatggat ggcgtgcttc caagcgtggt cggcggactg gttcggcaac
    15901 cggtgattgc ggtgccgacg agccggggct acggcgcaca cttcggcggg ttggccgcat
    15961 tgttgaccat gttgaattcc tgcgcggccg gagtcggagt catgaacatc gacaacggtt
    16021 tcggagccgg ttgcctcgcg catcgaatca acatggtggg nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn acatcgacaa cggtttcgga gccggttgcc tcgcgcatcg
    16201 aatcaacatg gtgggtgaag taaaaggtgc ggttccagta ggcaaaaatg attgagtctg
    16261 cggcgtttcc tcggccgact agcaggccgt taaaaagcca ttttgctgtc tctgaaaaaa
    16321 gtgatgcgtg agttgggaga gatattcaga acaccagcag gatggtcaaa aaggtcgtcc
    16381 agcgcggccg cagcgatgcg aggaggtgaa gcgtaccctt gcggtacgtt gagcctcgga
    16441 gcgacgcgag aacaaagctg gcggactttt tcaacatcct gctattcctt cacgacaacc
    16501 ttgccgcgtt tgggccatgc caacatctgc gtgcgttggc ccttgtctcc gttactcagt
    16561 aatttgaccc gcacttcata ttcataggac ccggccggca cttgctgctt gttgtggtcc
    16621 agtccgtccc acacgagatc cacctgcaca tgtttctgtg gcgtgtcccc agcggcgctg
    16681 gtggctgtct gcgcatgttc attgaccggc tgccggatcg acaggaagcg gagcgaggtc
    16741 atggaggagg aggtgacaag agcggacacc tccagcatca attgttcatc gacttccttg
    16801 ggcagttgca cggtcgcgat gaaatgaaag gggccgtcct gcgggcggta gggcatcggc
    16861 gcggcatgca gcgagagaat tttcaggtcc ggctccgggc gcgggacgct gcttttgccc
    16921 ttggttttgg ccgacgcatc catgggatgt cctcccagca gaagggccgc acctaagaga
    16981 aacaggaggc cgagtcgtac ggcacgcaat gcgcgaagga caatcgtaga gctgaatggt
    17041 gtggcattga tcatggtgtt gaagtcgcag cgtgtgggcc ggtggtccga tggaatgcgt
    17101 ctcttgattg gggataacac agcatcgaag ggctgtcaat gaacgagcgg aatgattgct
    17161 gtctggatgc ggctccggta agatgccgct cgctcgcagt ggatgatgaa tcgtaggagt
    17221 gacgtggcca cacatcttca tttcgactgt ttctccggca tcagcggcga catgaccttg
    17281 ggcgcgctgg tcgatgcggg attgccgttt aaagatctcg tgcgcgggct ggccttgttg
    17341 cggatcgagg ggtttcgcct gacgcggaaa cgggtcgagc gcggggccct cactgccact
    17401 aaagtcgatg tgctggtcga gaagggcttt cgcgccccgt taaccctggc gcagatcaaa
    17461 cggattctac tgaagagcgg cctgcccccg gtggtgaaag aaaagagcca ggccgtgttc
    17521 gatgtgctgg ctcacgctga ggggaaggcc catggcgtcg aaccctcgca tgtgcatttt
    17581 cacgaagtcg gcgtgatcga ttcgtttgtc gatgtggtcg gcggggtcct ggggctccat
    17641 ctcctggggg tgcagcgcgt gacggcctcc gcgattaatg tgggatcggg caccctcatg
    17701 tcggcacacg gatcgttgcc tgtgccggga ccggccgtgg ccgcgctggc ggtcggcctg
    17761 cctatctatg ccgggggtcc tgagcgggaa ctggcgactc cgaccggagt cgcgctggtg
    17821 cgaaccctgg cgacagaatt cggtcgcttg cctcatatgc aggtgcgccg ggtggggtat
    17881 ggggcgggaa ccgccgatcc ggcgcattgg ccgaatgtgt tacgagtgtt cgtcggtgaa
    17941 gaaccggccg ccgcaggttc ggtggaaacc atcgttgaac ttcagacgaa catcgacgat
    18001 ttgaatccgc aggtctacga gaccgtgttc gatcgggtgt ttgctgcggg agccgtggat
    18061 gcgaccttgg caccggtcac gatgaaaaag ggccggcccg ggaatgtact ctccgtgctg
    18121 gctccccagg agaaggtgga ggccgtgctg gccgtgttgt tcgccgacac gacggcgctc
    18181 ggggtcagga cccatgaggt gcagcgacgg gtgctgccac gacgatttgt cccggtacag
    18241 gtccatggcc acgaggtgtc tatcaaggtg gcggactccc aaccgggtcg gagcaaagcc
    18301 gctcctgagt atgaggattg taagcggatt gccgaacaga gcggccgtcc ggtgaaagac
    18361 attttggaag aggccatgca ggcctatcgg cgcacggcag gacatgcgaa aaaggaaaaa
    18421 gggagcaggt gaacacagga actgccgccg ttgtcttctt gtttgaggtg ccttcctcgc
    18481 gaggcctcca gtgacgatcc tgctctatgt gggattgttt gtggcctcgg tcgtcgtcac
    18541 cctcgccggg tgccatctct ttacgaacgc cattgaatgg ctcggcaagc gtctcaacat
    18601 ttctgaaggc gcggtcggga gcgtcttcgc ggcggtcggg acgaccctac cggagacgtc
    18661 gattccgatc atcgcgattt ttttcggggc cgggcgagag cagatggagg tcggtctggg
    18721 cgccattctc ggggcgccgt tcatgctcag cacgctggtc ctgcctattc tagcgctgct
    18781 gttgctgctc tacgcgaagc tcggtaagcg gacggcgaca ttcaagctca attacggtga
    18841 agtccgcgtc gatatcagct tcttcctcat cagttatgcg ctggcgttga cctgcgcggt
    18901 catcccctcc aagctctttc acgtggtggt cgcggcagtg ctgttgggca tgtacgtcta
    18961 ttacatgaag ctcaagttct cggccgaaga tgaagagggt gagggaggtg gtgaactgag
    19021 ccactgttgt tcgcacgccg gtcctctcgt ccgtcgtatc tggtgatcgg cgcgcaggga
    19081 atcgtgggcc tgctgggcct ggtcggtggg gcccatctgt tcgtgacggc cgcgaattcc
    19141 atttcagcgg aaatgcaggt ctcgccgctc atcctcgcgt tgctgatcgc tccgctggcc
    19201 actgagttgc cggagatgtc gaacagtttt ctctggttgt atcggaaaaa ggatcggctg
    19261 gccgtcggaa atgtcaccgg tgcgatggtg tttcagggct cgattcccgt ctctgtcggg
    19321 ttgctgggca ccgaatggac gctggggacg acggcgctgg cgaccatggt gctcgccgtg
    19381 ctcgctatga gcttgagtct attgcaggca gtctggtccg gacactggcg gccctggttg
    19441 ctcagcggta gcgcgctgct gtaccttggg tacacactgt tcttgtatgc acatggctcc
    19501 taaacgcacg accaaaacag cgcgtcctgt cctaggtctc acaatgggag atccggccgg
    19561 catcggaccg gaagtcatcg ccaaggcctt ggccgatagg gctctgacgc gtctctgcac
    19621 tcctgttgtc gtcggctccc gtcaggtcat ggaacggacg atcgcctggt tgaaattgcc
    19681 cttgcaggtg gtggacttca atccggcgaa cggccagcgg ttgaaggcgg ggcaggtggc
    19741 cgtggcagac ccgctggaaa gtccgttgcc gaaatttcgc atgggtgccg ctaccgcggt
    19801 gaccggtgca gcctccatcg cattcatcaa ggaagcggtg aagctggcgc aggcggggag
    19861 cctgggtggg atcgtcacgg ctcccatcaa caaagaagcc atgaacatgg ccggttttca
    19921 ctacccgggg cacacggaac tgctggcaga tctgaccaag accacagagt tcgggatgat
    19981 gattgtcggc gggccgctca aaatcatgtt caccaccacc catgtggcga tcaatgcctt
    20041 gtccccgctg ctgaccacgg aacggatcag caaggccatc cgcctcgcgc atctcgggtt
    20101 gacgaagtat ttcggcattg cgcgtcccag gatcggcgtg gcggcgttga atccgcacgc
    20161 aggagagcat ggtctgttcg gcaacgagga gtcgaccagc atcgcaccgg ccgtcaagct
    20221 ggccagggcg gcgggtatca aggccagtga tccgcttcca gccgataccc tgttcgggaa
    20281 ggccgctcgc ggcgactacg atggtgtggt ggcgatgtat catgatcagg ggctcattcc
    20341 tctgaagctg gtcgcgttcg gaacctgcgt gaacctgacc gtcgggctcc cgattatccg
    20401 gacctcagtg gatcatggaa cggcctacga catcgccggc aaaggggttg ccgagcatgg
    20461 gagcctgctg gaggccgtga aggtggcggc ccgcctggcc gaatcctggt caccggttac
    20521 caaaacgtcc cgataggaaa gaaggggaat acgtccatgg cagagacagg actttccgcg
    20581 gcgacgatga tcgatcagca ggtgaaggaa ttggatgcga tcgcgacaag gaccattcag
    20641 gatttcaaca ccaccttggg cactgaacgc atggggcagt ggaagatccg cacggtggcc
    20701 ctgctccagc agcatgccgg gcagaatgcg gccgatgagt tggcgcgaaa aaagccaggt
    20761 atggcgttca ccaacgattt gatcgaagag ttcaccgacg aggtggaaag ttatcgctcc
    20821 tacctcgtga cgcttgccaa acgcattcgg gccgcggccc ctccggccgc cggctaggcg
    20881 gtcgcacggt attatggcgt cctctcttcc cccagccctg aaacggctcg gtcagaattt
    20941 cctcatcgat ccgaacatta tccgcaagat cgtgtccctg gcggcgctcg gcccggagga
    21001 cactgtcttg gagatcggac cggggcgggg cgccctgacc gccggtttgt gcgccgaagc
    21061 gggacgcgtc attgccgttg agatcgatcc acagttgcag cctcatc
//
LOCUS       DAOQRM010000026        20979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1723_length_20979_cov_17.418467, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000026 DAOQRM010000000
VERSION     DAOQRM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20979
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1723_length_20979_cov_17.418467"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKK81_03990"
     CDS             complement(<1..377)
                     /locus_tag="PKK81_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32061.1"
                     /translation="MPWFPFVGMLCLAALLWSGSFVMAGNADHGHDAAVQEQGHQHAA
                     VRGELWEGSRQGIAYSEFNHHMAGVFLLLIGLSEVRQALGWPALVWTRFLLPGALTVG
                     GIFLLIWSDHDSWPIGSMTFAQT"
     gene            complement(488..1243)
                     /locus_tag="PKK81_03995"
     CDS             complement(488..1243)
                     /locus_tag="PKK81_03995"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HNV32062.1"
                     /translation="MKFVCLNCETYMTFQKVEKPGEGSLGVFFGCPSCEAKFSMVTNP
                     GETQMVASLGVKLGGRTETAEPFEMTRGTLKEEAQAGAGQMAAYLNEKIQGGQPVAAS
                     AKPSAPDAGGEKTSGGCPFSAMVAEMGLTSGGKPQNGNGTSEFTWTADAKEKLERLPS
                     FVKPMVQSSVEAYARKHGYKSITLQVMDDSKNDSPNGIAWTKDAEQRMDNIPDFIRPM
                     ARREIERIAKERGLVEITAQVMDEAKEKFMKFM"
     gene            complement(1270..2196)
                     /locus_tag="PKK81_04000"
     CDS             complement(1270..2196)
                     /locus_tag="PKK81_04000"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNV32063.1"
                     /translation="MTMAAGKDLKTILTKLQYSDDAKVVQQITAQMKQVQARMAGIKQ
                     KLVVMSGKGGVGKSMTTVNLALALARQGAKVGLLDVDLNGPCVPRMMGLHGQSLRMTP
                     EGAQPPVGPLGIKVASMDFFLDDASPVRWKGPMDLSPVWLGLMEMNVIREFLADVVWG
                     ELDYLLADLPPGAAADKPPVIAGFIPDLAGAIVVTTPSEVASDVVQKSVTYARDMGIR
                     VLGVVENMSEYRCPSCGAENELFEGNTEAMCEVLDLPLLGRIPFDRKFAKTFDKGQPL
                     LDPEYPTIQKYQEIVGRIQALLDYKKVLAEKL"
     gene            complement(2274..4121)
                     /locus_tag="PKK81_04005"
     CDS             complement(2274..4121)
                     /locus_tag="PKK81_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNV32064.1"
                     /translation="MTQHDQTKPQESLNIVIVGHVDHGKSTLVGRLYADTGSLPEGKV
                     EKVQAICRQQGKEFEYAFLFDAFLEEQEQGITIDTARTFFIWNGRQYIIIDAPGHKEF
                     LKNMISGAARAEAALLLIDALEGVREQSRKHGYLLSLLGVTQFAVVVNKMDLVGYRQD
                     VFDGIEKEYREFLGQFRAVPQQMIPVSAKMGDNIATRSSHMNWYQGPTVLETLALFKK
                     EQVRSEQPLRFPLQDVYKFDARRILAGRITAGRLKVGDQLVFSPSNKTAIVQSIEGFN
                     VDPPRGEAQAGQSVGITLDEQIFVERGEIASHRESIPLVSTAVRVNLFWMGRRPLEKG
                     RKYVLRLATREVACEVAAIHRIIDTADLAQLQESQAVAKNQVAELTLRVKAPLAFDLS
                     SSFEATGRFVLVDEYDIAGGGIITELVHDEQEGLREEARQREYAWLTGDVRAEDRATQ
                     YGHRAAIVLFTGSAQTGKTFLARRVEALLVADSRHAYLLEGENLLQGLDADLSAADPS
                     FAAERVRRYGEVARLLIDTGLIVVSTSKTFGINYQRMAEMIRTLVQPAPVIAVHMSRA
                     GEEPPPNTDLHFAGPQDFDAAARQILEELKRRGVLIQPSGTKSTIQYSI"
     gene            complement(4205..5002)
                     /gene="cysD"
                     /locus_tag="PKK81_04010"
     CDS             complement(4205..5002)
                     /gene="cysD"
                     /locus_tag="PKK81_04010"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNV32065.1"
                     /translation="MKHLRQLEDQSVYILREAYKHFNHLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSYKIPAMIEYRDRIAREWRLNLVVGQNKEALAAGMNHTLGRDV
                     CCTALKTTAMKNLVEEKGYTGIILGVRADEDSTRAKERYFSPRDKHGDWDFRDQPPEL
                     WDQFKTTFPPGTHIRIHPLLDWTEINIWEYIKHENIPFMDLYLDRGDGTRYRSLGCAP
                     CTMPIKSTAKTVDEIIAELRGTNVAERAGRAQDAGRGMEMLRKKGYM"
     gene            complement(5020..6087)
                     /locus_tag="PKK81_04015"
     CDS             complement(5020..6087)
                     /locus_tag="PKK81_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNV32066.1"
                     /translation="MQHLLAKVAKGQKTSKDLTWEEAKQAMRLMIEGTATPAQIGAFL
                     TAMRFKSESVTELAAFTATARQYVPPVPVRSGLGVVDVPVYAGKRETFHAILPAAIVA
                     AAAGAVLLLHGVDGPPDRLGISSVLKLLGIPVDMTAKSVGAELEKKGFAYLDLALYHP
                     PVSRFLEMRQELGVRNFFHPVARMLNPARATSQVIGLSHPPYFEKTIEALRMLSCPRA
                     LVIRGVEGDPELSIGNSTRLLELKDERITPFTFQPKDAGLSMATFREMAGFPVEQRER
                     EADLIKRLLANEIQGGQRDWVLLNAAMLLYAAGKGTSITGNLGAARSALESGQAKAKL
                     AELVAVAGSSAGNAPKVGISA"
     gene            complement(6129..6851)
                     /locus_tag="PKK81_04020"
     CDS             complement(6129..6851)
                     /locus_tag="PKK81_04020"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNV32067.1"
                     /translation="MKDTQKPAARPSDAELKTLSDSFESQQPWDLLEYALKTYRQRIV
                     LACSFGAEDVALVDMVHRIDPDAPLFYLDTDFLFPETFDVRDRIIARYGLKQAQVIQM
                     KSLLTPEQQAAQHGAMLWASKPDQCCELRKIEPLTRILGEYSAWITGIRRDQSPTRAN
                     AGLIEWDKKFNLVKVNPLARWSSENVWTYLQLHEVPYNTLHDRNYPSIGCTHCTAPVL
                     PGDDPRSGRWKNFGKTECGLHK"
     gene            complement(6857..>7528)
                     /locus_tag="PKK81_04025"
     CDS             complement(6857..>7528)
                     /locus_tag="PKK81_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNV32068.1"
                     /translation="DRMLEADQELYQAKLLVEKHQYALSVNKSYRAVLAAAKGLLVTE
                     GLDPATDAETFQEFDQRLASKGIVPASYKNLGAQVGDLGSKDATAEAATEKMAFAKRF
                     LAVCRAATEQMGKDLKLAQVKEEAVPTAAPAAVPASPVPVAAAQAPVYDLRGVACPMN
                     YVKTKLKLEMMDNGEQLEVWLDAGEPIRNVPMSLRNDGHRILDEGPLEPEAKHFKILV
                     EKVEG"
     assembly_gap    7529..7628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7629..9106)
                     /locus_tag="PKK81_04030"
     CDS             complement(<7629..9106)
                     /locus_tag="PKK81_04030"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNV32069.1"
                     /translation="MTPNTVALRPAIKTEPIPAHIVEEIETFEAEAQRVLGGDLSTDI
                     FKPFRLQYGIYGQRQPGVQMVRIKIPFGGLTANQLRQVADLADQYATGVGHVTTRQDI
                     QLHFVELKHVPEMMRLMASVGLTTREACANTVRNVTACHLAGVCQGEVFDVTPYAKTV
                     AYHLLRNPLNQSLPRKFKIALSGCRQDCALTPIHDIGLLAAKRADGTIGFRMVAGGGL
                     GSTPRMAQVLREFTPMDELLPTIEAVIKVFDTLGNRKNRNKARMKFVIEKLGFDEFKR
                     RWEAAYAAMGYAVPTHEPITLLEYADAPPLIMPTKAGVLSNGHGNGSGGGAVSLNGQV
                     SAFQAWRRTNVVPQRQAGFVTAAIKLPMGDLTGDQMWVVADLAERYSNGNIRTTINQN
                     MVIRWIPEGRLEEFYQELVQHSLGDPGAELVEDIIACPGTDTCGLGITSSKGMARALA
                     EVFPAGQVPEDLRDVSVKISGCHNSCAQHHIATIGLHGVGKRL"
     gene            complement(9161..9613)
                     /locus_tag="PKK81_04035"
     CDS             complement(9161..9613)
                     /locus_tag="PKK81_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNV32070.1"
                     /translation="MKVSLRATYGIIAAVDLALHHAEQPVCAKSIAKRQAIPARFLEQ
                     VLHAMKKAGVVTSQRGAQGGYVLSRKPSELSVADILDALEGPLLATNGEAGLKHSSSR
                     GAKQEALLAHIWDRVKRAELSVLSEVTVEELAKRQRALDAQHTLMYHI"
     gene            9954..10544
                     /locus_tag="PKK81_04040"
     CDS             9954..10544
                     /locus_tag="PKK81_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV32071.1"
                     /translation="MTLRSSTFNILLLSTDSEIQAHYKDLFGEQAVTIGRDGLSLPKE
                     PAKRAYDAVIVESKAQSAADLGKLFGGIDPARTLLVVGSRTVLKRTATSLHAAKAPKA
                     DLSLSNGNAPGQSVCLESFLEHKVGDFVKGMRNGSGRNLHPMLIAAVERPLILLTLRE
                     TKGNQIQAAELLGLNRNTLRKKILDLDIPVKRARAS"
     gene            10602..10913
                     /locus_tag="PKK81_04045"
     CDS             10602..10913
                     /locus_tag="PKK81_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNV32072.1"
                     /translation="MYPPVANHQRREAMTKEELIAKMANSAGITKVAATVALEAFTGA
                     VTTSLKKGKRVTLVNFGTFTISKRKARMGRNPRTGEALKIPAARIPKFSAGKELKAAV
                     K"
     gene            11048..11122
                     /locus_tag="PKK81_04050"
     tRNA            11048..11122
                     /locus_tag="PKK81_04050"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11080..11082,aa:Val,seq:gac)
     gene            11209..13164
                     /gene="thrS"
                     /locus_tag="PKK81_04055"
     CDS             11209..13164
                     /gene="thrS"
                     /locus_tag="PKK81_04055"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNV32073.1"
                     /translation="MASQFIHITLPDGTRKQVPAGCTVREALTPEGGRLDAKVLAAKI
                     NGEPMDLSRPLEQDATIEPLTFESAEGREVYRHSSTHIMAQAVKEVFPTAQLTIGPAL
                     DDGFYYDFAFDRPFTPEDLEKIEARAIEIMKRGLTVTRSELSKQDAIKFFQERGEGYK
                     VELINSFDDASPISLYRQGEFVDLCRGPHLPTTGYVGAFKLLSTGGAYWRGDERNPML
                     QRVYGTSFPTKKELDAHLAKLEEIKRRDHRKLGKELDLITIQDEIGPGLVLWHPKGSL
                     IRLLIENFWREQHIKDGYDLVYSPHVARLDLWKTSGHVDYYRENMFVSMKLEGSEYQL
                     KPMNCPFHIMIYKSHMRSYRDLPIRYGELGTVYRYERTGVLHGLLRVRGFTQDDAHLF
                     CRPDQIEAEVSRVLDFTFFVLGTFGFTEFEVYLSTRPEKSVGSEENWTIATNALEAAL
                     KSRGVAYEVDPGEGVFYGPKIDIKIKDVLGRSWQCSTVQVDFNNPERFKLAYTGEDGK
                     AHQPIMIHRALMGSIERFFGILIEHYAGAFPTWLAPVQAVVLTITDNQQEFAAKIVST
                     LKSHGFRVEADIRNEKIGFKIREAEKNKIPYMLVVGDKEVQSGMVSVRGRSGANHGSL
                     PIEAFLELLRTDANQTLRQTATHSQTR"
     gene            13168..13674
                     /gene="infC"
                     /locus_tag="PKK81_04060"
     CDS             13168..13674
                     /gene="infC"
                     /locus_tag="PKK81_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNV32074.1"
                     /translation="MVPKLRVNREIRIREVRVIGPEGEQLGILPTVEAFNKAQEGGYD
                     LVEVAPTSQPPVCRIMDYGKYKFELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIK
                     IRQIKEFLADGNKTKVTLTYRGREMANQEMGRTMMASVIKECTEAGTVEFAPRMEGRS
                     LIMILAPK"
     gene            13714..13905
                     /gene="rpmI"
                     /locus_tag="PKK81_04065"
     CDS             13714..13905
                     /gene="rpmI"
                     /locus_tag="PKK81_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003851299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNV32075.1"
                     /translation="MKMKTHSGAKKRFRRTGTGKLVRRKAGGRHLLTGKPRDRKRNLK
                     GAVEVSSASTPALNRIIPQ"
     gene            13951..14319
                     /gene="rplT"
                     /locus_tag="PKK81_04070"
     CDS             13951..14319
                     /gene="rplT"
                     /locus_tag="PKK81_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNV32076.1"
                     /translation="MPRTKGGPKTRQRRKKRLKLAKGQYGAKSRLFRTATESVDKGQA
                     YAYVGRKNRKRDFRQLWIARISAATRLHGIAYSRFMNALKKANILLDRKVLSDMAIRD
                     MAGFEKLVGVAKQQLATVAS"
     gene            complement(14379..16097)
                     /gene="pheT"
                     /locus_tag="PKK81_04075"
     CDS             complement(14379..16097)
                     /gene="pheT"
                     /locus_tag="PKK81_04075"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNV32077.1"
                     /translation="MPTISLQRDDLEALIAGPDEEPARIPLDQLEQWLMLVKGELKGH
                     NSETGELRIELQDSNRPDLWCCEGIARQIRIKQRGSAISYPFFKKPKGVAGKLVVAQG
                     MDQIRPYVAACTAVGYRVTASGLTQLIQTQEKLADIFGRKRRSVSIGLYRLAPIVFPV
                     SYDLVKPDDVRFTPLGMETMMTLREILMVHPKGVEYGGIVGGHDRLPVLRDAKGQVLS
                     FPPIINSREIGEVQVGDHELFVEVTGTDLSMVALTLNIFAANLADRGAVITPVEIHSP
                     VKTNFGRRWSTPIDFGESRTIPLKAIESALGEALGGKEVASALKSYGYTVKSAGQKVA
                     VQLPPYRNDLLHTVDVVEDVAISQGYARFAPVMPSQFTVGGLSRLEQMADRVRHLMVG
                     LEFQEIISNIMGSHQDFCTRMRLDGTEWAKVVEVDNVMSLSYSCLRQWITPSLLQVET
                     NSSRAFYPHRMFEVGEVAIPDASADVGSRTVTMLGAVIAHAGAHFSEIHSCLDLLLYY
                     LDRPFTLEPVDHPSFLDGRAGKIVSNGRVIGLLGELHPEVLEHWQIGVPAVALEVEID
                     RLLEEV"
     gene            complement(16110..17654)
                     /locus_tag="PKK81_04080"
     CDS             complement(16110..17654)
                     /locus_tag="PKK81_04080"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNV32078.1"
                     /translation="MDNLHPLESKVLLALTRQPDAPPTLDQLAASTGLEPSQLSMAVE
                     WLLAKSLIAVQAETVAHIASLTPIGELFFEKYAPIERVLSAAREAGQTGKRLTIQDIQ
                     AKEALEPSDVSKAVGTLKKEGAILIVQGGCIESTGRNSATAEAMRSLLQELRSGQREL
                     QSFPEPLRSVLQQHAVKRGNAREPFRIDERVTRSFVLTPAGQEVAEQLSRDGVAEEVS
                     QLTPELLKEGAWRTKRFRKYTISLRAPRIAAGKRHPYREFLDLVKLKLVSMGFQEMRG
                     TLVETEFWNMDALFMPQFHPARDIHDVYFVKNPTHARTIAEPYLTQVTQVHEKGTGAG
                     STGWGYAFDAERAKRLVLRSQGTAVSARTLAAGASVPGKYFSIARCFRYDQVDATHAT
                     DFFQVEGIVLGADINFRTLLGLLNLFAREVAQAKEVKFLPAYFPFTEPSVEMHVRHPK
                     LGWMELGGAGLFRPEVTTPLGVSVPVIAWGLGLDRMAMVALGIHDIRDLFSADLEFIR
                     TMRGSF"
     gene            complement(17682..18386)
                     /gene="rpe"
                     /locus_tag="PKK81_04085"
     CDS             complement(17682..18386)
                     /gene="rpe"
                     /locus_tag="PKK81_04085"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNV32079.1"
                     /translation="MAGRTVRIAPSILSADFARLAEEVARVEDAGADWLHIDVMDGHF
                     VPNLTVGPPIVEALRKVTTLPLDVHLMMTNPDAFIGEFAEAGADYLTVHVETCPHLHR
                     TVQSIKERGVKAGVTLNPATPAGTLSEIVRDADLILIMSVNPGFGGQKFIASSLRKIA
                     EVRALIDRTGSRALLEVDGGVKPDNVQEILAAGAEVLVAGSAVFSTDDYAAAITALRA
                     GHEPAARTARVATAQR"
     gene            complement(18386..19798)
                     /gene="rsmB"
                     /locus_tag="PKK81_04090"
     CDS             complement(18386..19798)
                     /gene="rsmB"
                     /locus_tag="PKK81_04090"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNV32080.1"
                     /translation="MASDLPPSKSRGVVSAGRRAAMKALLAIDTAGMLGDDLFDQVST
                     REALDLRDRAFMVELVRGVLRYRATLDWRLGLLSDRRITKLPTLVQTILRLGAYQLLY
                     LDRVPDSAAVNESVQMTKQQSRRLGRDWSGFVNAVLRALLRSPEPVWPDAGSHPVEAL
                     AVRYSCPAWLVERWCHRLGVERAEALCGASVEAPPLTLRVNTLRTSRAALLGDLAAAQ
                     VEAGPTSISEVGIQLARSCSVIDLPGYAEGRFYVEDEAGQLIPLLLDVQPGQRVLDAC
                     SAPGGKATHLAALMENRGEIVAVDRASARLDLVMANCRRLGVKILTPLAGDLRALVGA
                     GIVSHPMLSRPFDRILLDAPCSGLGVLRRHPEGKWYKTPESIAQHRQMQVELLAVTSR
                     LLRPGGVLVYSTCSIEPEETESIIDEFCQAHHQFQRESIAPWLPPAGLPFVTPRGDLS
                     TMANSNRMDLFFAARVRRSE"
     gene            complement(19859..20809)
                     /gene="fmt"
                     /locus_tag="PKK81_04095"
     CDS             complement(19859..20809)
                     /gene="fmt"
                     /locus_tag="PKK81_04095"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNV32081.1"
                     /translation="MRIVFMGTPDFAVPSLEALLKSEHQVVGVVTQPDRPKGRGQEVV
                     FSPVKVVCQREGIPVLQPLKMKDPAFLDALRRWTPDVIAVTAYGRILPPAILTLPPRG
                     CINVHGSLLPKYRGAGPIQWAVIRGEQQTGITTMFMAEGMDTGDMLLQETVEIRADDT
                     AGTLAPRLAEVGGRLLVETLRRLEAGTLTPQPQDDAQATMAPLLKKEDGVVDWTLSAM
                     EIANRVRGLSPWPGAYTHVNGERWVLWRVAVGLETSGAVPGTVTKVSKDRVEVATGDG
                     TIQLIEIQPSNSRRMTMAQYLAGHRLAEGLKLQAIPLSQS"
     gene            20897..20973
                     /locus_tag="PKK81_04100"
     tRNA            20897..20973
                     /locus_tag="PKK81_04100"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20931..20933,aa:Met,seq:cat)
BASE COUNT         4447 a         6571 c         5737 g         4124 t
ORIGIN      
        1 aacgtctgcg cgaaggtcat cgacccgatg ggccacgagt catgatcgct ccaaatgaga
       61 agaaaaatac ctcccaccgt caaggcgccg ggcagtaaaa acctcgtcca aaccaacgcc
      121 ggccatccca aggcctgacg gacttcactc agcccgatca gcaacaggaa gacacccgcc
      181 atgtgatgat tgaattccga ataggcaatg ccctgtcgcg acccctccca cagttctccc
      241 ctgacagccg catgctgatg tccctgctcc tgaacggccg cgtcatgccc gtgatcggca
      301 ttcccagcca tcacaaatga accgctccac agcagggcgg ccagacacag catccccacg
      361 aacggaaacc atggcatcag gaatctcgga caatgaatcg gtcgagagaa aaacaccggc
      421 aggaattgaa aggcccatcc ggactgccgg atgggccgcg cactcgtctc acgcatgcat
      481 tcgtcggcta cataaacttc atgaacttct ctttggcttc atccatcact tgcgccgtga
      541 tctcgaccaa gccgcgctcc ttggcaatcc gctcaatttc ccggcgggcc atcggacgaa
      601 tgaagtcagg aatgttatcc atgcgttgtt cggcatcctt ggtccaagca ataccgttgg
      661 gcgaatcgtt cttggaatcg tccatgacct gcagcgtaat gctcttatac ccatgcttgc
      721 gcgcataggc ttccacacta ctctgaacca ttggcttcac aaaagacggc aagcgctcca
      781 acttctcctt ggcgtctgca gtccatgtga attcgctcgt tccgtttcca ttctgcggct
      841 tgccgcctga agttaatccc atttccgcga ccatcgccga gaaggggcac ccaccggacg
      901 tcttctctcc accggcatcc ggcgcgctgg gcttcgcaga agccgcaacc ggttgcccgc
      961 cctgaatctt ctcgttgaga taggcagcca tctgccctgc gccggcctgt gcctcctctt
     1021 tcagcgtccc gcgagtcatc tcgaacggtt ctgccgtctc tgtgcggcct ccgagcttca
     1081 caccgagcga ggcaaccatt tgggtctctc caggattcgt caccatggag aacttcgcct
     1141 cgcaggatgg acagccgaag aacacgccga gtgagccctc accgggtttt tcgaccttct
     1201 gaaacgtcat gtaggtctcg cagttcaggc aaacgaactt cataggatcc cccctccggg
     1261 tcggtgtgct tacagctttt ccgccaggac ttttttgtaa tccagtaacg cttgaatccg
     1321 cccgacgatt tcttgatatt tctggatcgt cggatactca ggatcgagga gcggctgccc
     1381 cttatcgaac gttttggcaa acttacgatc gaacgggata cggccaagca aaggcaggtc
     1441 cagcacctcg cacattgcct ccgtgttccc ctcgaacaac tcattctcgg cgccgcagga
     1501 tggacaccgg tattcactca tgttttctac gacacctaac acccggatac ccatgtcccg
     1561 cgcgtaggtg acggatttct gcaccacgtc ggaagcgact tcggacggtg tcgtgacgac
     1621 gatcgcaccg gccaggtcgg gaatgaatcc cgcgatgacc ggcggtttat cagcagcggc
     1681 acccggcggg agatcggcga ggagataatc caactctccc cagaccacat cggccaggaa
     1741 ctcgcggatc acgttcattt ccatgaggcc gagccagacc gggctcagat ccatcgggcc
     1801 cttccaccgc accggcgagg catcatcgag gaaaaaatcc atcgacgcca ccttgatgcc
     1861 taagggaccg acgggaggct gcgccccttc cggagtcatt cttagcgact gaccgtgtaa
     1921 acccatcatc cgtggaacgc aggggccgtt caaatcgaca tcgagcagtc cgaccttcgc
     1981 cccctgtcgc gcaagggcca acgccaggtt gacggtcgtc atgctcttgc cgactccgcc
     2041 cttgccgctc atcaccacca gcttctgctt gatgccggcc attcgagcct gcacctgctt
     2101 catctgggca gtgatctgct gcaccacttt cgcgtcgtcg gaatactgaa gcttcgtgag
     2161 gatcgtcttg agatccttgc cggcagccat ggtcatgaaa aaccctcgga attgttgatg
     2221 aagaacgcta ccacaggggt ttcaatagag tcaaaccgcc ccgcccccat cgactagatg
     2281 gaatattgga tggtggactt cgttccggag ggctggatga gaaccccccg ccgcttcaac
     2341 tcctccagga tctgacgggc agcggcgtca aaatcctggg ggcccgcgaa atgcagatcg
     2401 gtattcggtg gcggttcttc ccccgccctg ctcatgtgca cggcaatcac cggagccggc
     2461 tgaaccaacg tccgtatcat ctcggccatt cgctgataat tgataccgaa ggttttgctg
     2521 gtggacacga caatgagccc cgtatcgatg agcaacctgg caacctctcc ataccggcgc
     2581 acgcgctcgg cggcaaagga cggatcggcc gctgacaaat ccgcatccaa tccctgcagc
     2641 agattttcgc cttccagcag gtatgcgtgg cggctatcgg cgaccaataa cgcttcgacc
     2701 cgtcgggcga gaaacgtctt tcccgtctgg gctgaaccgg tgaacaacac gatggcggcc
     2761 cgatgcccgt attgcgtcgc gcggtcctct gcccggacat cgcctgtaag ccacgcatat
     2821 tcccgctgcc gtgcttcctc ccgcagccct tcctgctcgt catgcaccaa ctcggtaatg
     2881 atcccgcccc cggcaatgtc gtactcatcg accagcacga accggcctgt ggcttcgaat
     2941 gaggacgaga gatcgaacgc gagcggcgct ttcacacgca gggtcagttc ggcgacctga
     3001 ttcttcgcca ccgcctggct ttcctgaagc tgcgcgagat cggcggtatc gataatccga
     3061 tgaatcgcgg ccacctcgca ggcgacttca cgcgtggcga ggcgcaagac gtacttccgc
     3121 cccttctcca gcggccgcct gcccatccaa aacaagttca cacgaaccgc cgtggaaacg
     3181 agcggaatgg attcccgatg ggacgcgatt tcgccacgct ccacgaaaat ctgttcgtcg
     3241 agcgtgatgc cgaccgattg accggcctgt gcctcacccc gcgggggatc gacattgaat
     3301 ccttcaatcg attgcacgat ggctgtcttg ttggacggcg aaaagaccag ttgatcaccg
     3361 actttcaagc ggcccgccgt aatgcgaccg gccagaatcc gccgcgcatc gaacttatag
     3421 acgtcctgca gtgggaatcg gagcggctgt tccgagcgaa cctgctcctt cttgaagagg
     3481 gccagcgtct ccagcaccgt gggcccctga taccagttca tatggctgct gcgcgtcgcg
     3541 atgttgtcac ccatcttcgc gctgaccgga atcatctgtt gggggaccgc ccggaactga
     3601 cccaaaaatt cccgatattc cttctcgatc ccgtcgaaca catcctgccg atacccgacc
     3661 agatccattt tattgaccac caccgcaaac tgcgtcacac ccagcagcga caggaggtag
     3721 ccatgcttcc tggactgctc gcgcacgcct tccaacgcgt cgatgagcaa caacgcggct
     3781 tccgcccggg ccgcgcccga gatcatgttc ttcaaaaact ctttatgtcc cggtgcgtcg
     3841 ataatgatgt actgccgccc gttccagata aagaacgtgc gcgccgtatc gatcgtgatg
     3901 ccctgctcct gctcttcaag aaacgcgtcg aacaggaacg catactcaaa ttccttgccc
     3961 tgttggcggc agatcgcctg caccttttcc actttgcctt ccggcaagga gcccgtatcg
     4021 gcatagagtc gtcccaccag cgtggacttc ccgtgatcca catggcccac gatgacgatg
     4081 ttgagactct cctgcggctt ggtctggtcg tgctgtgtca tatgattcac tcgttcaatg
     4141 gaaccccggt tcatgatcgt gatcgttcga atgcccgccg gctatccaac cggcgtgggc
     4201 cgcctcacat gtaccccttc ttccggagca tctccatgcc ccgccccgcg tcctgcgcgc
     4261 gcccggcccg ctcggccaca ttggtcccgc ggagttccgc gataatctca tccaccgtct
     4321 ttgccgtgga tttgatcggc atggtacagg gcgcgcagcc caaacttcga taccgtgtgc
     4381 catcccccct gtccaggtat agatccatga aagggatgtt ttcatgcttg atgtattccc
     4441 agatgttgat ctcggtccaa tcaaggagcg gatgaatccg gatatgcgtg cccggcggaa
     4501 aggtggtctt gaactgatcc cacagttccg gcggctggtc gcgaaaatcc caatccccgt
     4561 gtttgtcgcg cggggaaaaa tatcgctcct tcgccctggt actgtcttca tccgcccgaa
     4621 caccgagaat aatgccggtg tagccctttt cttcgaccag attcttcatg gccgtcgtct
     4681 ttaaggcggt acagcagaca tcacgcccga gcgtgtggtt catcccggcg gccaaggcct
     4741 ctttgttctg acccaccacc agattgaggc gccactcgcg ggcaatccgg tcgcgatatt
     4801 cgatcatcgc aggaatcttg taactcgtat ccacgtggag caagggaaac ggcacatgcc
     4861 cgaaaaaggc cttgcgggcc agccacaaga gcaccgtcga atccttcccc atcgaccaga
     4921 gcatggcgag gtgattgaaa tgcttgtagg cctcccgaag gatatagacg ctttgatctt
     4981 cgagttgacg taaatgtttc atggttcaca ctcttccgtt tacgctgaaa tgccgacctt
     5041 cggcgcgttt cccgcgcttg atccggcgac cgccaccaat tcagccagtt tggccttggc
     5101 ctgaccggac tccaacgcgc ttctggcggc cccaagattt ccagtaatcg acgtcccctt
     5161 gccggctgca tagagcaaca tggccgcatt gaggaggacc cagtcccgtt gcccgccctg
     5221 aatctcgttg gcgaggagcc gcttgatcag atcggcctcc cgctcccgct gctcgaccgg
     5281 gaacccggcc atttcccgga aggtcgccat cgacaagccc gcatccttcg gctggaaggt
     5341 aaatggagtg atgcgttcgt ccttgagttc caagagccgc gtggaattgc cgatcgagag
     5401 ttccggatcc ccttcgactc cgcgaatcac caacgcacgg ggacaactca acatacgcag
     5461 cgcctcgatc gtcttttcga agtaaggcgg atgcgacagc ccgatcactt gcgaggtcgc
     5521 gcgggcgggg ttgagcatac gggccacagg atgaaagaaa ttgcgcacgc ccaactcctg
     5581 ccgcatttcc agaaaccggc tcaccggggg gtgatacagc gccaggtcca ggtacgcgaa
     5641 tcccttcttt tccaattcgg ctccgaccga cttggcggtc atatcgacag gaatgcccaa
     5701 gagcttcagc accgacgaga taccgagccg gtccggaggc ccatccactc cgtgcaacag
     5761 caagacggct ccggcggccg ccgcgacgat ggccgcgggc aggatggcgt gaaaggtttc
     5821 ccgtttcccc gcatagacgg gcacatcgac gactccgagc ccggaacgga ccggcaccgg
     5881 cggcacatat tggcgcgcgg tcgcagtgaa ggccgctaat tccgtgaccg attccgactt
     5941 gaatcgcatg gccgtgagga acgcccctat ttgagcaggg gtggcggttc cctcgatcat
     6001 gaggcgcatg gcctgcttgg cttcttccca cgtgagatcc ttggatgttt tttgaccttt
     6061 agcgactttg gcgagcagat gttgcatgga tggtaatgac cttgtctaat ttcagggtga
     6121 cgatgatgtt acttgtgcag cccgcactcg gtcttgccga aattcttcca ccgaccggaa
     6181 cgcggatcgt ctcccggaag gacaggcgcc gtacaatggg tacagccgat gctggggtaa
     6241 ttgcggtcgt gtaacgtgtt atagggcacc tcatggagct gcagatacgt ccagacgttt
     6301 tcactgctcc atcgggccag gggattcact ttgaccaggt tgaatttctt gtcccactcg
     6361 atcagcccgg cgtttgccct tgtgggagac tggtctcgcc ggatcccggt gatccaggcc
     6421 gagtactcgc cgagaatacg agtgagcggt tcgattttcc tgagctcaca gcactgatcc
     6481 ggcttgctgg cccacaacat ggcgccgtgc tgggcagcct gctgttccgg cgtcaacaga
     6541 gacttcattt gaatgacctg cgcctgcttc aacccgtacc gcgcgataat gcgatcacgc
     6601 acgtcgaagg tttcaggaaa cagaaaatcg gtatccagat aaaaaagcgg tgcgtccgga
     6661 tcgatacgat gcaccatgtc caccagggcg acatcttccg ccccgaaact gcaggccagc
     6721 acaatgcgct gccggtaggt cttcaacgcg tactccagca ggtcccatgg ctgctgagat
     6781 tcaaacgaat cactcagtgt cttcagctct gcgtcggagg gtcgtgcagc cggcttctgt
     6841 gtgtctttca cccttcttac ccctcgacct tttccacaag aattttgaaa tgcttggcct
     6901 caggctccaa cggcccctcg tcgaggatcc tgtgtccgtc gtttcgcaag ctcatcggca
     6961 cattgcgaat gggttctccg gcgtcgagcc agacctccag ctgctcgccg ttgtccatca
     7021 tttccaattt caatttcgtc ttcacataat tcatcgggca ggccacgccg cgcagatcgt
     7081 acacaggcgc ctgagccgcg gcaacgggta caggcgaagc aggcaccgca gcaggagctg
     7141 cagtcggcac ggcttcttcc ttcacctggg ccaacttcag atctttgccc atttgttccg
     7201 tggccgcccg acagacagcg agaaatcgct tggcgaaggc cattttctcc gtcgccgcct
     7261 cggcagtcgc gtccttagag ccaaggtcgc cgacttgcgc gccaagattc ttatagctgg
     7321 ccgggacgat gcccttgctg gccagtcgtt gatcgaactc ctggaaggtt tcggcatcgg
     7381 tggccggatc gaggccttcg gtcaccaaaa gacctttcgc agccgccagt accgcccgat
     7441 aggacttatt caccgacaag gcatactgat gcttctcgac cagcaatttc gcctgataga
     7501 gttcctgatc ggcctctaac atacggtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnag acgtttgccg actccgtgca acccgatggt ggcaatatgg tgctgggcgc
     7681 aggaattatg gcacccgctg atcttcacgc tcacatcccg aagatcttcc ggaacctgac
     7741 cggcgggaaa cacttccgcc agcgccctgg ccatgccctt agacgaggta attcccaatc
     7801 cgcaggtatc ggtgcccggg caggcgatga tgtcttcgac gagctctgcg ccaggatcgc
     7861 ccaagctgtg ctgcaccagc tcctgataga actcctccag ccgcccctcg ggaatccacc
     7921 ggatgaccat gttctggttg atggtcgtcc gaatattacc gttcgaatac cgttcggcca
     7981 agtctgcgac gacccacatt tggtcgcccg tcagatcgcc catcggcaac ttgatcgccg
     8041 ccgtcacaaa acccgcttgc cgctgtggca cgacattcgt gcgcctccag gcctggaacg
     8101 cggagacctg gccattgagt gacacggcac cacccccgct accgttgcca tgaccgttgg
     8161 acaggacgcc tgccttggtc ggcataatga gcggaggcgc atcggcatat tcgagtaacg
     8221 tgatcggctc gtgcgtcggc acggcatagc ccatggccgc gtaggccgct tcccagcgac
     8281 gcttgaattc gtcgaagccg agcttctcga tgacgaactt catgcgggcc ttgttgcgat
     8341 tcttgcggtt cccgagcgtg tcgaacacct tgatcaccgc ctcgatggtg gggagtaatt
     8401 cgtccatcgg ggtaaattct cgtaacacct gagccatgcg cggcgtcgag cccaatccac
     8461 cgcccgccac catccggaaa ccgatggttc cgtcggcccg cttcgccgcg agcaacccga
     8521 tgtcgtggat gggtgtgagc gcgcaatcct gccgacatcc ggacagcgca atcttgaact
     8581 tgcgcgggag actctgattc agcggattgc gcagcagatg ataggccacc gtcttcgcgt
     8641 agggcgtgac atcgaacact tcgccttggc acacacccgc cagatgacag gccgtgacgt
     8701 tccgtaccgt attcgcgcag gcctcgcgcg tggtcaaccc cacggaggcc atcagacgca
     8761 tcatctcggg gacgtgtttg agttccacga agtgcagctg aatgtcctgc cgtgtcgtga
     8821 catgacccac gccggtggca tactggtcgg cgagatcggc cacttgccgc agctggttcg
     8881 ccgtcagccc accgaacggg atcttaatcc gcaccatctg gacaccaggt tgccgctggc
     8941 cgtaaatgcc atattgcagc cggaagggct tgaagatgtc ggtggagagg tctccgccca
     9001 ggacacgttg ggcttccgcc tcaaacgttt cgatttcttc gactatatgg gccggaatgg
     9061 gctccgtttt gatcgctgga cggagagcca ctgtattagg agtcataata acgacctcgt
     9121 cggttaggaa gtaatgtgcg tgcgcatgat tcaggccgct tcagatgtgg tacatcaacg
     9181 tgtgctgcgc gtcgagggcc cgctggcgct tggccaactc ctcgaccgtg acttccgaaa
     9241 gaacgctcaa ttccgcccgt tttacgcgat cccaaatgtg ggcgagcagc gcctcctgct
     9301 tggcgccacg cgaagacgaa tgtttcagac cggcctctcc atttgtcgcg agaagcggcc
     9361 cctccaatgc atcgaggatg tccgccacgg agagctccga gggcttacgg ctcaacacgt
     9421 acccgccttg agccccgcgc tgacttgtca ccactccggc ttttttcatc gcatgaagca
     9481 cctgctcgag gaaccgggcc ggaatcgcct gtcgcttggc gatcgacttg gcgcaaaccg
     9541 gctgttcggc atggtggagc gcaagatcta cggcggcaat aattccgtag gtcgctcgaa
     9601 ggctcacctt catttgcgag tattccgatg gggattcatg gatctctata ccttgcgcgc
     9661 aactttcctg tcaagcaagg gtagatgcac atcaaaccat catgccgttg ggaaccaagc
     9721 gctcatgacg tgtctgagac agccacccgt gcgagcgatt catattgaag aaccgtggct
     9781 cgccagtgca gtgccttcag ccgcgatgaa ctggtggtag gagccagtca cgaccgtgcc
     9841 cgcgcaccat gagttcaccc ctgtcgtctg cgcgtcatta caatgcattt ttcgtcattg
     9901 acatcatcta ggggctgagc aataatcccg ccctcttctt cctcggtacc ttcatgacgc
     9961 tgcgttcatc aacatttaat attctcttgc tcagcacaga cagcgagatc caggctcact
    10021 acaaggacct gttcggcgag caagcggtca ccatcggccg tgacggattg tcgcttccga
    10081 aggaaccggc aaagcgtgcg tacgatgccg tcatcgtgga atcgaaagca cagtctgctg
    10141 cagaccttgg gaaattgttt ggggggatcg atcctgcgcg cacgctccta gtcgtaggct
    10201 ctcgcaccgt gctgaaacgg acggcaacgt cgctccacgc ggccaaagcc cccaaggcag
    10261 acctgtcgct atccaatgga aatgcaccgg ggcaatcggt ctgtctggaa tcttttctgg
    10321 aacataaagt cggcgatttc gtcaaaggga tgcgcaacgg ctcgggaaga aacctccatc
    10381 cgatgctgat cgccgccgtc gaacgcccgt tgatcttgct gaccctgcgg gagaccaagg
    10441 ggaaccagat tcaagccgca gagctgctcg gactgaatcg aaatacgctg agaaaaaaga
    10501 ttctcgatct ggatattccg gtcaaacgcg ccagggcgag ctagattcgc gcgcatctca
    10561 gctcatcagc acctcaccag aggcgtagaa tctgtacgcc catatatcca ccggtcgcca
    10621 accaccaacg gagggaagca atgaccaagg aagaattgat cgcgaaaatg gcgaacagcg
    10681 ccggtatcac caaagtcgcc gcgacagtgg cactcgaagc cttcaccggc gcagtaacca
    10741 cgtcgctcaa gaagggaaaa cgcgtgacgc tggtgaactt cgggacgttc acgatttcaa
    10801 aacgcaaggc caggatggga cgcaatccac gaaccggcga agccttgaag attcctgccg
    10861 cccgcattcc caagttttcg gccggcaaag aactcaaagc agcggtcaag tagacccttg
    10921 ctgataaacc agaggacgcc tggccggtga gacggcttcc cactctggaa ggctgagaga
    10981 aaagcctgtc ttgccgccgg gtttccttga cactcttggt gaggctgtat tagcatcgcc
    11041 gggcgatagg cgcgtagctc agggggagag cgctaccttg acacggtaga ggtcgacggt
    11101 tcaagaccgt ccgcgcctac catttttgtt cgcccacaca gttcgtgatc cgactaccgg
    11161 cagcatggtg gtcggcacgg cgttgtattt tttgtaggat tgaccggagt ggcctcacag
    11221 ttcattcaca ttacccttcc tgacggaact cgaaaacaag taccagcagg atgcaccgtc
    11281 cgagaggccc tcactccgga aggtggacgc ctcgacgcca aggtactggc agccaagatc
    11341 aacggcgagc caatggactt gtcgcgtccg ttagagcagg acgccaccat cgagcctctg
    11401 accttcgagt ctgccgaagg gcgcgaggtc tatcgccaca gcagcaccca catcatggcg
    11461 caggccgtga aggaagtgtt cccgacggcc cagctgacga tcggcccggc attggacgat
    11521 gggttctatt acgacttcgc gttcgaccgt ccgttcacgc ctgaagatct ggaaaagatc
    11581 gaggcgcgcg ccatcgagat tatgaaacgt ggtctgacgg ttacccgcag cgagctgtcc
    11641 aagcaggacg ccatcaaatt tttccaggaa cgcggggaag gctacaaagt cgagctaatc
    11701 aacagcttcg acgatgcctc gcctatttcg ctctaccgtc agggggaatt cgtcgacctc
    11761 tgccgcgggc cgcacctccc cacgaccggg tatgtgggtg ccttcaagct gctctcgacc
    11821 ggaggcgcct attggcgtgg cgacgagcgg aacccgatgc tgcagcgggt ctacggcacg
    11881 tccttcccga ccaaaaaaga actcgatgcc catctcgcga agctagaaga gatcaagcgc
    11941 cgcgaccatc ggaaactcgg caaggagctc gacctgatta cgattcagga cgagatcggt
    12001 cccggcttgg ttctgtggca ccccaagggc tcgctgatcc ggctgctcat cgaaaacttc
    12061 tggcgggagc aacatatcaa ggacggctac gacctggtgt attcacctca tgtcgcccgg
    12121 ctggacctgt ggaagaccag cggccacgtc gattactacc gcgaaaacat gttcgtctcg
    12181 atgaagctgg aaggcagcga gtatcaactc aagccgatga actgcccctt ccatatcatg
    12241 atctacaaat cccacatgcg gagttatcgg gatttgccga tccgttatgg cgaattaggt
    12301 accgtctacc gctatgaacg cacgggtgtg ctgcacggcc tgctgcgtgt ccgcgggttt
    12361 acccaggacg atgcgcatct gttttgccgg cccgatcaga tcgaggccga agtcagccgc
    12421 gtactggact ttacattttt cgtcctcggc accttcggct ttacagagtt tgaagtgtat
    12481 ctttcgaccc gcccggagaa atccgtcggc tccgaagaaa actggaccat cgccactaat
    12541 gcgttggaag ctgcgctcaa gagccgcggt gtggcctacg aagtggatcc cggcgaaggg
    12601 gtcttctatg gcccgaagat cgatatcaaa attaaagacg tcctcggccg ctcctggcaa
    12661 tgctcgacgg tccaggtcga cttcaacaat ccggagcggt tcaagctggc gtacaccggc
    12721 gaagacggca aggcgcatca gccgatcatg attcatcggg cgctcatggg ctccatcgaa
    12781 cgcttcttcg gcatcctgat cgagcactat gcgggcgcct tcccgacctg gctggccccc
    12841 gtacaggcgg tggtcctcac gatcacggac aaccagcaag aatttgcagc gaagatcgtc
    12901 tccacgctca agagccacgg ctttcgagtt gaggcggaca tccgcaacga gaagatcggc
    12961 tttaagatcc gcgaggcgga aaagaacaag attccttata tgctggtcgt gggagacaag
    13021 gaagtgcaga gtggcatggt gtcggttcgc ggccggagtg gcgcgaatca cgggagcctg
    13081 ccgattgaag cgttcctgga actccttcgc acagatgcga atcaaacatt gcgtcaaacg
    13141 gcaacccact cacaaacgag gtgacctatc gtccctaaat tacgcgtaaa ccgggaaatc
    13201 aggattcggg aagttcgagt catcggtcct gaaggcgagc aactaggaat actgccgacc
    13261 gtagaggcct ttaacaaggc ccaggagggg ggctacgacc ttgttgaggt ggctccgacc
    13321 tcgcagccgc cggtttgccg catcatggac tatgggaagt acaaattcga actcagcaag
    13381 aaagatcacc aaagccggcg tcaccagaag tctacgcagg tcaaagagat taagcttcgt
    13441 ccccgcaccg acaagcatga cctcgagatc aagatccgcc agatcaagga attcctggcc
    13501 gacggcaaca agaccaaagt gacgttgacc tatcgcggac gcgaaatggc caatcaggag
    13561 atgggccgaa caatgatggc cagcgtcatt aaggaatgta cggaagcagg gaccgtggag
    13621 tttgcgccac gaatggaagg gcggagcctg atcatgattt tggctcccaa atagtgtgcc
    13681 gagtgcacac aaggaaggaa tgagagcatg aaaataaaaa tgaaaaccca cagtggcgcg
    13741 aagaagcggt ttcgacgcac aggaacgggg aagctggttc gccggaaggc cggcggtcgg
    13801 catttgctca ccgggaagcc acgcgaccgc aagcggaacc tgaagggggc cgttgaggtg
    13861 tcctccgcct cgaccccagc actgaaccgc atcattccgc agtaacgacg attgtgccgt
    13921 gaacaggatc tgaaaaagga gtagctcccg atgcctcgta caaaaggtgg tccaaaaaca
    13981 cgacagcggc gaaagaagcg cctgaaactg gcgaaaggcc agtatggcgc caagagccgg
    14041 ctgttccgaa cagcaaccga atcggtcgac aaggggcagg cctatgccta cgtcggacga
    14101 aagaatcgca agcgcgattt ccgccagctc tggatcgcac gcatcagcgc tgcgacccgc
    14161 ctgcatggca tcgcgtatag ccgtttcatg aacgccctga agaaggcgaa tatcctgctg
    14221 gatcggaaag tgttgtccga tatggcgatc cgggatatgg cgggatttga gaaactcgtc
    14281 ggggtggcca agcagcaact cgcaactgtc gcgagctaac ggttacgtcc gcacgaacgt
    14341 tctgtggcgg gagaatcctg atcgaacgga ttttcccgtc acacctcttc cagtagtcga
    14401 tcgatctcca cctccagcgc caccgccggg accccgattt gccagtgctc cagcacctcg
    14461 ggatgcaact cgcccaacaa cccaatgacc cgaccattcg atacgatctt ccccgctcga
    14521 ccatcgagaa atgagggatg atccaccggc tccagggtaa aggggcgatc caggtaatac
    14581 agcagcagat ccagacagga atgaatttcc gagaagtgcg cgccggcatg ggcaatcacg
    14641 gcacccaaca tcgtcacggt ccgggatccg acatcggcac tcgcatcagg aatagccact
    14701 tccccgacct caaacatgcg gtgcggatag aaggcccggc ttgaattggt ttccacctgc
    14761 aacaacgatg gcgtaatcca ctggcgcagg caggagtagc tgagagacat cacgttatcc
    14821 acttccacca ccttcgccca ctcggtgcca tccagacgca tgcgtgtgca aaagtcctga
    14881 tgagagccca tgatgttgga aatgatttcc tggaattcca gacccaccat gagatgccga
    14941 acccggtcgg ccatctgctc caaacgcgac agcccaccca ccgtaaactg cgagggcatg
    15001 acgggggcaa acctggcgta tccctgactg atggccacat cctccaccac atcgaccgta
    15061 tgcagcagat cgttgcggta gggcggtagt tgcacagcca ccttttgccc ggccgacttt
    15121 acggtgtacc cataggactt gagcgccgag gcgacttcct tgccacccag ggcctctccc
    15181 aacgccgact cgatggcctt gagaggaatt gtccgggatt ccccgaaatc gatcggcgta
    15241 ctccagcgtc gcccgaagtt ggttttcacc ggcgagtgaa tttcgaccgg cgtgatcacc
    15301 gcgccgcgat ccgcgagatt ggcggcaaaa atattcaggg tcagcgcgac catcgaaaga
    15361 tcggtcccgg tcacctcgac gaacaattca tgatccccga cctggacctc gccgatttcc
    15421 cggctgttga tgatcggggg aaaggacaac acctgtccct tggcgtcccg cagcacaggg
    15481 agccggtcgt ggccgccgac aatgccgcca tactccacgc ctttgggatg aaccatgagg
    15541 atctcccgta gcgtcatcat ggtttccatg ccgagcggcg tgaatcgcac gtcgtccggc
    15601 ttgaccagat cgtaggagac ggggaacacg atgggagcca aacggtataa gccgatcgag
    15661 accgaccgcc gcttacgccc aaaaatatcg gctaactttt cctgcgtttg gatgagctgc
    15721 gtcaggccgg atgccgtcac gcgatacccg accgccgtgc aagcggccac gtacggacga
    15781 atctggtcca ttccctgcgc cacaaccagc ttccccgcca ctcccttcgg cttcttgaag
    15841 aagggatagg aaatcgccga gccacgctgc ttgatccgga tctgcctggc gatgccttca
    15901 caacaccaca agtccggacg attgctgtct tgcaactcga tccgcagctc gccggtctct
    15961 gaattgtgcc ccttcagctc tcccttcacc aacatcagcc attgctcaag ctgatcgagc
    16021 ggaatccgcg ccggctcttc gtccggtccg gcaattaagg cttcgagatc gtctcgttga
    16081 agggaaatcg tgggcatcgt gtcgtcgtcc taaaaacttc cccgcatggt gcgaatgaat
    16141 tcgaggtcgg ccgagaacaa atcgcgaatg tcgtgaatgc ccagcgccac catcgccata
    16201 cgatcgaggc caagacccca ggcaatgacc ggcacagaga caccgagtgg ggtcgtcact
    16261 tcgggccgga ataatccggc gccgcccaat tccatccacc ccagcttcgg atggcgcaca
    16321 tgcatttcga ccgacggctc ggtgaacgga aaataggccg gtagaaattt gacttccttg
    16381 gcttgcgcca cttcccgggc gaacagattc aacaatccga gcagggtccg gaaattgata
    16441 tccgccccta acacgatgcc ttccacctga aagaagtcgg tggcatgggt cgcgtccacc
    16501 tggtcgtaac gaaagcagcg ggcaatcgaa aaatatttcc ccggcacgga agcgccggct
    16561 gccaatgtcc tcgccgacac cgcggtgcct tgactccgca acaccagccg tttcgcccgt
    16621 tcggcatcga aggcatagcc ccaccccgtc gatccggccc ccgtcccttt ttcatgcacc
    16681 tgcgtcacct gcgtcaggta cggctcggcg attgtgcggg catgggtcgg atttttcacg
    16741 aaatagacat catgaatgtc acgggccgga tgaaactgcg gcatgaacag cgcgtccata
    16801 ttccagaact cggtctcaac gagcgtcccg cgcatttcct ggaaacccat actgaccagc
    16861 ttgagtttga cgagatccag aaactcgcga tagggatggc gtttcccggc cgcgattctc
    16921 ggggcccgca aactgatcgt atatttccga aaccgcttcg tccgccaggc accttccttc
    16981 agcaactccg gcgtcagctg cgagacttcc tccgccacac catcccgcga cagctgctcc
    17041 gccacctcct gcccggcagg ggtcagcaca aatgaccggg ttacccgctc atcgatccgg
    17101 aacggctcac gcgcattccc acgcttcacc gcatgttgct ggagcacgct ccgaagtggt
    17161 tcgggaaaag actgcaactc ccgctggccg ctccgcaatt cctgcaacaa cgagcgcatg
    17221 gcctcggccg tggcactatt gcggccggta ctttcaatgc acccgccctg gacaatcagg
    17281 atcgcgccct ctttcttgag ggtgccgacc gccttgctca catcggacgg ctcgagtgct
    17341 tccttagcct gaatatcctg aatcgtgaga cgtttccccg tctggccggc ttcccgcgcc
    17401 gcagagagca ctcgttcaat cggggcatat ttctcaaaaa acaattcgcc gatcggggtc
    17461 agagatgcga tatgcgcgac ggtttcagcc tgcacggcga tcagcgattt ggcgagcagc
    17521 cattcgacgg ccatgctcaa ctgcgacggc tccagcccgg ttgatgccgc aagttgatcg
    17581 agcgtaggag gcgcgtccgg ctgccgggtc aacgccagca gcactttgct ttcgagggga
    17641 tgaaggttat ccatgaacaa gggtgtggtc cgacgcctct tctatcgctg agcggttgca
    17701 accctcgcgg tgcgagcggc aggctcatgt ccggctctga gcgccgtaat ggcagcggca
    17761 tagtcatccg tggaaaatac ggccgacccc gcaaccagca cctcggcgcc ggcggcgaga
    17821 atctcttgca cgttgtccgg cttcacgcct ccgtctactt ccagaagcgc ccggctcccg
    17881 gtgcggtcga tcagagccct gacctcggca atcttgcgta acgacgaggc aataaacttc
    17941 tgcccgccga acccgggatt cacggacatg atgaggatga gatcggcatc acggacgatt
    18001 tccgaaaggg taccggccgg cgtggcagga ttcaacgtca cacctgcctt cacgccccgc
    18061 tctttgatcg actgcaccgt ccgatgcagg tgcgggcagg tttccacatg cacggtcaga
    18121 taatcggcgc ccgcttcggc aaactccccg atgaacgcat ccggattcgt catcatcaga
    18181 tgcacatcca acggcagggt ggtgactttt ctcaaggctt cgacgatcgg cggtccgacc
    18241 gtcaggttcg gcacgaagtg cccgtccatg acatcgatgt gcaaccagtc ggctccggcg
    18301 tcctccaccc gcgccacttc ttcggccagg cgcgcgaagt ccgccgacaa aatggagggg
    18361 gcaatacgta ccgtccgacc ggccattact cgctcctccg cacacgggcg gcgaaaaaca
    18421 gatccatcct gttactatta gccatcgtag acagatcccc tcgaggggtc acgaacggca
    18481 gtccggccgg gggcaaccag ggcgcgatcg actcacgctg aaattgatga tgagcctgac
    18541 aaaactcgtc gatgatcgac tcggtttctt ccggttcgat cgagcaggta ctatagacca
    18601 acacccctcc aggccgcaag agacggctcg tcaccgcaag caattccacc tgcatctgcc
    18661 gatgctgcgc gatggactcc ggcgtcttat accacttccc ttccggatga cgccgcaaca
    18721 caccgaggcc gctgcacgga gcatccagca gaatgcggtc gaacggtcgt gacaacatcg
    18781 gatgggacac gatccctgca ccgaccagcg cgcgcaagtc gccggccagg ggcgtcagga
    18841 tcttcactcc gagacggcgg caattggcca tcacaagatc gagtcgcgcc gaggcccgat
    18901 ccaccgccac aatctctccc cgattctcca tgagcgccgc caggtgtgtg gcctttccac
    18961 ccggcgctga gcaggcgtcc agcacccgtt gccccggctg cacatccagc aacaacggaa
    19021 tcagctgccc ggcttcatcc tccacataga accgtccctc cgcgtatccc ggcagatcga
    19081 taacggaaca ggagcgcgcc agttgaatgc cgacttcgct gatcgacgtc gggcctgcct
    19141 ccacctgcgc cgcagccaag tcgcccagca atgccgcgcg ggaggttcga agcgtattca
    19201 cacgtagcgt cagcggaggc gcctccaccg acgcaccgca caaggcctca gcgcgttcga
    19261 cgcccaagcg atgacaccaa cgttccacga gccaggccgg acaggaatat cgcacggcca
    19321 aggcctcaac gggatggctg cctgcatcgg gccagaccgg ttcgggtgat cgaagcagag
    19381 ccctcagcac cgcattcacg aagccgctcc aatcccgacc cagacgacgg ctctgctgct
    19441 tcgtcatctg cacagactcg ttcacggcag cggaatccgg aacccggtcc aagtacagga
    19501 gttgataggc gccgagacgc agaatggtct gaaccagggt gggaagtttc gtaatacgcc
    19561 gatccgacaa cagacctaag cgccagtcga gagtggcccg ataccgcaac accccgcgca
    19621 ccagctcgac catgaaggcc cgatcgcgca gatccaacgc ctcgcgtgtg gacacctgat
    19681 cgaacagatc gtctcccaac atgcccgctg tgtcgatcgc cagcaacgcc ttcatggccg
    19741 cgcgtcgccc ggcagataca accccgcgtg attttgacgg gggaagatca gatgccatgt
    19801 tcggaaagga tgatcatggc gagaagatag tcgatagaat gcgatgatgg gcgcgcgatt
    19861 agctttgcga tagagggata gcttgcagct tgaggccctc ggccagtcga tggccggcca
    19921 gatattgcgc catcgtcatc cgcctgctat tggatggttg aatctcgatg agctggatcg
    19981 tcccgtcgcc ggtcgccacc tcaacccgat ccttgctcac cttcgtcacg gttccgggaa
    20041 ccgcgccgga ggtctccagc cccaccgcca cgcgccagag cacccaccgt tccccgttca
    20101 catgggtata cgcgccgggc catggagaca ggccgcgcac acgattcgca atctccatag
    20161 ccgacaaggt ccaatcgacc acaccgtctt ccttcttgag caatggcgcc atggtcgcct
    20221 gtgcgtcatc ctgcggttgc ggcgtcagcg tgccggcttc aaggcggcgc agggtttcaa
    20281 ccaacagtcg cccgccgacc tcggccaacc gaggggccag cgtgccggcc gtatcgtcgg
    20341 cacggatctc caccgtttcc tgtaacaaca tgtcgccggt atccatccct tcagccataa
    20401 acatggtcgt aatgccggtt tgctgttccc ccctgatgac ggcccattga ataggccctg
    20461 ccccacggta cttcgggagg agcgacccat gtacgttgat gcagccgcga ggcggcaacg
    20521 tcagaatggc cgggggaaga atgcgtccat aggccgtgac ggcaatgaca tccggcgtcc
    20581 accgtcgcaa ggcgtcgaga aaggcgggat ctttcatctt gaggggctgg agcaccggaa
    20641 tcccttcccg ctggcagaca actttcacgg gcgagaagac cacttcctgc ccgcgcccct
    20701 tcggccgatc aggctgagtg accacaccga ccacctgatg ttccgactta agcaaggcct
    20761 ccagagaagg cacggcaaag tcaggagtcc ccataaatac gatacgcatg ctcacgcttt
    20821 atcattctgc cctcgcgaat gcaattctcc ccggacgttc cttgactctc gtttcacacg
    20881 cctgttagta tccggtcgcg gggtggagca gcctggtagc tcgttgggct cataacccaa
    20941 aggtcgtcgg ttcaaatccg gcccccgcaa ccaaccttc
//
LOCUS       DAOQRM010000027        20476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1794_length_20476_cov_16.906175, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000027 DAOQRM010000000
VERSION     DAOQRM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20476
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1794_length_20476_cov_16.906175"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PKK81_04105"
     CDS             complement(<1..701)
                     /locus_tag="PKK81_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV32082.1"
                     /translation="MELLKGKVAIVTGSSSGIGRAIAERLAQDGATVVVNYHVHEEKA
                     REVAAGIQASGGAAAVIQADMSRVADAQRLVQETVQQFHRLDILVNNAGRFIPKKLVE
                     TTETEFDAILALNAKGPYFAMQAAAKVLSDGGRIVNISSALTHLKFPGATAHLGSKAA
                     LEQYGKGLAQELAPRGITVNTVLPGFTDTGVLTEPYREMGLQSSPFKRLGLPSDVADV
                     VAFLVSEQARWLTGQV"
     gene            810..1493
                     /locus_tag="PKK81_04110"
     CDS             810..1493
                     /locus_tag="PKK81_04110"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV32083.1"
                     /translation="MSIRLEQTVPFGRSLREYELMFSLSAADRRSRILDCAAGPSSVN
                     AELTAAGGDVRSIDPLYQFGGAEIQRQFFSTLDHVIEQVRATPQNWVWSYHRDPEDLR
                     RNRIAVMEQFVADYSGGTGTGRYLCGALPNLPFETDRFDLALSSHFLFLYSDHFDAAF
                     HLAAIRALLRVAREVRIFPLLALRAERSQHLEPVCEQLRQEGHHLSIERVGYELQRGG
                     NEMLRLRRA"
     gene            complement(1504..2109)
                     /locus_tag="PKK81_04115"
     CDS             complement(1504..2109)
                     /locus_tag="PKK81_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV32084.1"
                     /translation="MEKPADVTHPIHALLQRRWSPRAFADRMVEPEQLQSLFEAARWA
                     PSSNNEQPWHFIVGTKADPSAHDRLVACLKEGNRKWAFRAPVLILSVARLNFEDEGTP
                     NRHAWHDTGMAALSLCLQATALGLVTHQMAGFEIEKARTDLGIPAGYEPVAMIAVGYP
                     GDPASLPDYLRERELKPRERKAVTEFVSHGRWNGPPEWPTR"
     gene            complement(2136..2699)
                     /locus_tag="PKK81_04120"
     CDS             complement(2136..2699)
                     /locus_tag="PKK81_04120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HNV32085.1"
                     /translation="MAEKTEKNKMLGGELYRASDKELLDERRHAQQRLARYNGLPDSG
                     HDMRTTLLRHFLSAVGEGTVVLPTFTCDYGYNISLGRNVFINYHCIFLDCAPIAIGDD
                     VQIGPAVQLYTAQHPIDAKVRRSGLESASAIRIGNDVWIGGGALVLPGVTIGDRSIVG
                     AGSVVVHNVPPDSLVVGNPARIVRTLV"
     gene            complement(2721..>3208)
                     /locus_tag="PKK81_04125"
     CDS             complement(2721..>3208)
                     /locus_tag="PKK81_04125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV32086.1"
                     /translation="KDEAERIQRVHADAFAKQAPSLRVLPGAQELLNALTAAHVPYAI
                     ATSGRLQSAQHTLKLLKLPPGTPVVTRDQVKRAKPDPDLFLAAAEQLHMPIARCIVVG
                     DSVWDLLAARRASALGVGLLSGGYGREELERAGAYRTYDDPADLLRHLDECGVRPEPP
                     V"
     assembly_gap    3209..3308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3309..3574)
                     /locus_tag="PKK81_04130"
     CDS             complement(<3309..3574)
                     /locus_tag="PKK81_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV32087.1"
                     /translation="MTVSQDPDSVAFLFDLDGTLVDSVYQHVLAWREATQAAGIELPV
                     WRIHRQIGMSGGLMLHALWRETGRPVSKDEAERIQRVHADAFAK"
     gene            complement(3580..4005)
                     /locus_tag="PKK81_04135"
     CDS             complement(3580..4005)
                     /locus_tag="PKK81_04135"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNV32088.1"
                     /translation="MKRNASAKWQGDLKTGKGTVSTDSGVLSETQYSFSTRFENGTGT
                     NPEELIAAAHAGCFTMALSGQLGAANLVAEQLATTATVTMEKVEAGWTVTGVHLSVTG
                     KVPKADAASWEKATAAAKAGCPISRLLNATITMDAKLEN"
     gene            complement(4107..6503)
                     /locus_tag="PKK81_04140"
     CDS             complement(4107..6503)
                     /locus_tag="PKK81_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HNV32089.1"
                     /translation="MWIPRDKSILSGPDQGEEEAKPAPPLLSANLPFAIDPSWVLVES
                     GFEPGWEHEIESIFTMANGYVGTRGSLAERTSLSSPATFLAGAFEASEAPGGTLELTK
                     LQDWTQLRVLVEGVPLSLETGTTLDHRRILDLQHGLLFRTWRHEDEQGRVTALTCVRA
                     VCHHDRHLLLQSVTITPENYSGTISIESFTEGPQPGTQWSSRVEGEAAVIVRRTDRGT
                     TIAMASHNKLRHENSQEGAGNSPVEGFAEQWTWQAQRGEAARFDRSVVVYSSREVGDP
                     AAAAGEHVRRVSEASLNAHMLQHAEAWEDRWRASGVEVAGDEMAQRALRFALYHLNSA
                     VNPKDEYASIGARALTGSTYKGHVFWDTEIYLLPFYTFTDPPAARALLQYRFHTLPAA
                     RDRARRLGYQGALFAWESADTGEDVTPDTVIAPDGRVVQVLTGIQEHHISADVAYAVW
                     QYWQATADEAFFLAAGADILIETARFWASRGRLEPDGQYHIRTVIGPDEYHESVDDNA
                     YTNVMAQWNLERAADTVDWLRSRRSDVWPIICERLQVKPDEPDSWRRTAELMTTGFDR
                     PTNLFEQFSGFFQLEEVELQAYRTLPVPIDVTLGRDRTQKSKVVKQADVVALSALLWE
                     RFPSAVHEANFRYYEPRTAHGSSLSPALHALVSARLGDSDLAARYFRDAAAIDLSNHR
                     GNAAGGVHIATLGGLWQAAVFGMGGVRLREDGLVIDPHLPSNWDRLSFPLQWRGRSVS
                     VTIDHEPGQVTVDLRTGEPMTIELSHGPIQEIISNHLYVAHRTGSGWGAWQESRRRSG
                     "
     gene            6607..7383
                     /locus_tag="PKK81_04145"
     CDS             6607..7383
                     /locus_tag="PKK81_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HNV32090.1"
                     /translation="MTQGATGEQPFHPRSIDLTKFDAVLFDLDGVLTKTAVVHAAAWK
                     RLFDEYLQAKASREQVPFRPFDVMLDYQRYVDGKLRYDGVRAFLESRNIVLPYGTAHD
                     GPEQETVQGLGNKKDGYFQAHLKQHGIELYDDAVRFLRTLRAQGVRTAVVSASKHTAA
                     VLQKTGLADYFDTRVDGIESERLHLAGKPAPDGFLEAARRLQVDPPRAIVVEDAVAGV
                     QAGRNGGFGLVIGVDRTGHAEELTRNGAHLVTSDLAELCV"
     gene            complement(7524..7862)
                     /locus_tag="PKK81_04150"
     CDS             complement(7524..7862)
                     /locus_tag="PKK81_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32091.1"
                     /translation="MRHMKGFFATLFTAGLIFSAGPLFAEVDDGSHITITSPKDGAQV
                     GETFELTYELTKGSKAAHGHVYLDGEPQKKFPGTFKGLNKGKHEIKVQAATHDHDHLA
                     ATDTITVEVQ"
     gene            complement(7965..8405)
                     /locus_tag="PKK81_04155"
     CDS             complement(7965..8405)
                     /locus_tag="PKK81_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNV32092.1"
                     /translation="MSMQNTFRVVVGTLVLAGALVGAGCHRHHTPSERADWMTGKIAK
                     HLDLDDQQKAKLAVVKDEVIAARAESQQEHRVIMEDVIAQVQSDRLDQSKVAQLFERH
                     QAGQTRLMQRVLPKVAEWHATLRQDQKAEAVEHLRKWMERYGEH"
     gene            complement(8437..8733)
                     /locus_tag="PKK81_04160"
     CDS             complement(8437..8733)
                     /locus_tag="PKK81_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNV32093.1"
                     /translation="MPVTLINVFSVPHTKEAEFVQWWQEVKEHITKQEGFISGKFHTS
                     IKPDSKFNYINVAIWENEEVYWKAYEKSVTPMKAKLGELGVEMTPALYRVAFEY"
     gene            complement(8860..9777)
                     /locus_tag="PKK81_04165"
     CDS             complement(8860..9777)
                     /locus_tag="PKK81_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32094.1"
                     /translation="MTRTMVILGSMVLSLSMAVPYGFAAEGGGKKATHMKVSGVVSNV
                     QSGITTVKTPWGTMKIASTLTPKNLEVGEEVDMQVNENNAVIDVHRKGDKAHSHRFVT
                     GNLAYTSPDKKEIKVWTPEGEKAIDVQTGKSKLSALEEGAPVTIELNEAGKMIDIHRF
                     TVEMSFDEHPRTKPGYVIQVDGTVVKFDASLAYVKTPAGQYTLLKKYAPADAAVGDHV
                     SLWINEEGMVIDVHGKDKAKAGTHRLIFGKLIRTGPNKNQIKLATPEGEKVFPLERME
                     IKTKPIADGDNIVVELNEEGTVIDLRKAQ"
     gene            complement(9791..10078)
                     /locus_tag="PKK81_04170"
     CDS             complement(9791..10078)
                     /locus_tag="PKK81_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV32095.1"
                     /translation="MLKITVSNTNPGTTFVLSGRLAGPWVRELRDCWLKQEFDGPVPC
                     HVDLREVTFIDEAGTTLLSQMYRMGVTIQASGCLTRAIVQNLGKTGVHNHG"
     gene            complement(10140..10640)
                     /locus_tag="PKK81_04175"
     CDS             complement(10140..10640)
                     /locus_tag="PKK81_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNV32096.1"
                     /translation="MYSFFRTDESWAGLILRVVLGGVIFAHGAQKLLGWFGGNGFDGT
                     MGFFTNVMHLPWIVAFLVIIGESIGSLALIAGLLTRFTAASFIVIMLGAIMTVHLPQG
                     FFMNWFGQQQGEGFEFHLLVIAMSLVLLVVGGGKWSLDGVIARWLGEESPALKAREAS
                     FALRTF"
     gene            complement(10662..10793)
                     /locus_tag="PKK81_04180"
     CDS             complement(10662..10793)
                     /locus_tag="PKK81_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32097.1"
                     /translation="MKDMTLWGWWYGMLVTLPLFVAGLFLKDRMASAVPQWVRSHRR"
     gene            complement(11119..12162)
                     /locus_tag="PKK81_04185"
     CDS             complement(11119..12162)
                     /locus_tag="PKK81_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNV32098.1"
                     /translation="MHTLLVMVMVSLLMMVAPAQADTPGAWSKGAPFPEPSEELVGLS
                     TGGKFYVFGGLGPGWIPQGLVYEYDPSANKWTKNKPMALPAHHVAFTELNGKFYAFGG
                     FVPPQSGPPAWVPINNAWEYDPAHDSWKALAAMPSARGSAVAATVNGKIYVIGGAAMH
                     PGSKEAALYPTRPHRSVGTVEEYDPKTNSWSERSSMPTARNHAAVGAVNNKIYVIGGR
                     LGSAFIFTASNTNVVEEYDPATDQWGLVRARMPTERSGGAWGVYNGRIYVAGGEHQDG
                     HLMAAFRALEAYDPATNSWSELPMMPMPRHGLAGAVVGHRLHLASGDIQSAGITGMHV
                     VTDAHDIFEFSDR"
     gene            complement(12351..13505)
                     /locus_tag="PKK81_04190"
     CDS             complement(12351..13505)
                     /locus_tag="PKK81_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV32099.1"
                     /translation="MTARQQAVTWGRRLAAMTWKEILQLSRDVPLLLFLLYSFSLSVV
                     VSGAGITMQLTNAELLVHDADHSHSSRELIHRFQPPYFKFAGEISDPREGLRQLDQGR
                     VMVLLEIPPRFHEALMSGERTAVQLLVDTTNAPQGLSAAGYSVRIAGLFGTETGLASA
                     GLSGAEGAIPRVSSAHRVWFNPTQDERWFQSIAHVLRMTTIFAVLLPAAALVREKERG
                     TVEQLLVSPLSPFQIMFSKVLAMSGVILIALSLALYGVLHPVFHVPMKGSTGLFFLLT
                     TLHVFTTAGFGLVAATIAKNQAQVGMMTLFVVGPMLLLSGLTSPYESMPQWVQTVMTF
                     SPLRYYIDITYGVMLKGAGLDLLWKPVVAMLLLGGALFGFGMWRFRRQFQ"
     gene            complement(13516..14646)
                     /locus_tag="PKK81_04195"
     CDS             complement(13516..14646)
                     /locus_tag="PKK81_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV32100.1"
                     /translation="MALKEWKETTRDRLFLLLAFLLPALWLVVFGYGLNLDVENIPFA
                     VLDRDRSELSRDYLHRFMQSRYFSFQGYADDERALDRLLTETKIRAAIIVPERFQEQL
                     AAGEPASVQTLLDGTFPLHTDIAKGYVIAINQAFTEERLIEYLRRTRGLTQEQADALV
                     RPLTVEVRYLYNEEVRSTWSMVPALVMFTLMLASPLLTALGVVREKETGSIYNIYSST
                     VSRAEFLTGKLLPYIVISLVNVCVLWLMAVGLFQVPFKGSFLLFFSASVLFVCCTTGI
                     GLLISLLVQTQMAALIITMVVAMIPTILFSGLLVPVASLTRGAKVQAHLYPAMYYTNI
                     VRGSFLKGVGADVLWTDLLALAMFAVALSGLTYRLFTKRPKT"
     gene            complement(14667..>15816)
                     /locus_tag="PKK81_04200"
     CDS             complement(14667..>15816)
                     /locus_tag="PKK81_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV32101.1"
                     /translation="REPEAAVAGIRQALENLPVEHIHTEEPELEDVVVALLLKEREEQ
                     PGESAGSDQSARPAGRQQLDGLAVEAKELIRDFGSFRAVDRVSFDMKQGEIFGLLGAN
                     GAGKTTVIKMLNGIVPPTAGEGRVAGVDMRTAGGAIKERIGYMSQAFSLYLDLTVIEN
                     IRLFAGIYGLARREAQQRMEWIVEMAGLTGYEDDRTGRLPMGVRQRLALGCALVHSPR
                     VLFLDEPTSGVDPIGRRRFWELLSRLAREEGVAILITTHYLSEAEHCDRLALMYAGRI
                     VAEGTPAHLKKQVERDIGQLVEMVVDKPGIALAHVVAAGFAGAALFGTKIHVLSRDPG
                     RDEERLRDVLARSGIAVEAVRTRMLSLEDVFVSRVMALEQAAQKEVSA"
     assembly_gap    15817..15916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15917..16801)
                     /locus_tag="PKK81_04205"
     CDS             complement(<15917..16801)
                     /locus_tag="PKK81_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV32102.1"
                     /translation="MTKPAASSPEVVVRVSGFVKCYKQHLAVDRLDLTVKRGEIYGLI
                     GPDGAGKSSVMKAIAGVLRYDGGSAEVFGTLVDSERAAERVKPRIGFLPQGLGLNLYP
                     ELSVEENIDFFARLRLVPEPELRARKSRLLAITRLDRFRSRLMKQLSGGMKQKLGLIC
                     TLIHEPELAILDEPTTGVDPVSRRDFWAILAEWQADKGMTALVSTAYMDEAARFHRLS
                     FLSHGRVLASGTPAEVQSLVPGLVVTFESSPQLEAVARLKRRYAQVESLGPLLHVFTP
                     EREPEAAVAGIRQALENLP"
     gene            complement(16798..17901)
                     /locus_tag="PKK81_04210"
     CDS             complement(16798..17901)
                     /locus_tag="PKK81_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV32103.1"
                     /translation="MTLRTSTIAAAVLASVLIAGYVILDRLVWQDGLPEGLIQANGRI
                     EGDHVTVSSKFPGRIVDLAAREGSQVHKGHVLIRLDDAQTRAKVDQAARLVDSMMAQV
                     EAAHTALAVLNLEVPLSIEAVAAKVASMRAVIRKAQAVEYEARRDTDRIRALLPEQAV
                     SQQQVDQAEARWKVAVTEIAVAKAALDQASKELAQAELGWGRIRSKEAEVAALERQRD
                     QADATFAEARSILDDLTIVAPTNGTVTTRMVDVGEVVATGAPLLELVDLDRLYLQVYV
                     PELQIGKVRLDLPARIHTDAFSDRSFEATVRYIASKAEFTPKEVQTPDERVKLIYAVR
                     LYLTENPDHRLTPGLPADAVIRWKDDVAWSKPR"
     gene            complement(17905..18345)
                     /locus_tag="PKK81_04215"
     CDS             complement(17905..18345)
                     /locus_tag="PKK81_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNV32104.1"
                     /translation="MKMQSTVRVAIGTLVIAGALVGAGCHRHHTPTERADWMTGKIAK
                     HLDLDEQQKAKLAAVKDEAMAVRAESEKERKATMEEVIAQVQSDRLDQAKLARLFDQH
                     QAGQTRLMQRVLPKLAEWHATLRPEQKAEAVEHLRKWMDRYGDH"
     gene            complement(18439..>20476)
                     /locus_tag="PKK81_04220"
     CDS             complement(18439..>20476)
                     /locus_tag="PKK81_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV32105.1"
                     /translation="RYGALLQVVEAIASHADLQGLVQDLARLLPLLVPVNFVGLSLYD
                     AQRDMMRLHVLRANVPADIIGGQESHPADTPAGLVWQTQQPVILSDLAEEHRWPKLIA
                     LMREDGVQSCCLVPLTSAVRQLGALAFSSLKKDAYRVSDLALMQQIGRQVAVAVENVL
                     NREAAAAARQDVERQRDRFSLLLRMTNTMVSKLDLREVFSAVSLCLREMVPQEYASLI
                     LCDGKNGRVRLHALDFPENQGALTEGAVSDVAGSLAGLALERRRPAVANNRPDLTAFS
                     HAVPQLLVMKGFHSMCSLPLLSQDRVIGCLNLASRQEQAFGEQDVEFLSQVAGQIALA
                     VDNALAYQEITELKDRLTEEKLYLEEEIRLEHKFDDIIGDSRALKQVLGQVEIVAPTQ
                     ATVLIQGETGTGKELIARAIHRLSGRSQRTFVKLNCAAIPTGLLESELFGHERGAFTG
                     AIAQKVGRFELAHGGTIFLDEVGEIPLELQSKLLRVLQEQEFERLGSTRTIHVDIRLI
                     AATNRDLAKMVEDHEFRSDLYYRLNVFPITLPPLRERREDIPVLVRYFTQHYAARMKK
                     HIETIPGPSLEALSRYHWPGNIRELENLVERAVILTQGTHLQVPLLELKIEARQAPAP
                     TPTLQDAEREQVLRVLRDTHWVIGGPDGAAARLGLKRTTLTSKIKKLGLSRPRE"
BASE COUNT         3950 a         6571 c         5695 g         4060 t
ORIGIN      
        1 acctgaccgg tcagccaccg tgcctgctca ctgaccaaaa atgccaccac atcagccacg
       61 tcggacggaa gcccgagccg tttgaagggt gacgattgta gacccatctc gcggtatggc
      121 tccgtcaaca caccggtatc ggtaaagccc ggcaacacgg tattgacggt aatgccgcgt
      181 ggcgccagtt cctgcgcgag ccccttgcca tactgttcca gcgccgcctt gctccccaga
      241 tgcgccgtgg cgccgggaaa cttcaggtgg gtgagcgccg acgaaatatt cacaatgcgc
      301 ccaccgtcgc tgagcacctt tgccgccgcc tgcatcgcaa agtacggccc cttcgcattc
      361 agcgcaagaa tggcatcgaa ctccgtctcc gtcgtctcca ccaacttctt tggaatgaac
      421 cggcccgcat tgttgaccag aatatcgagg cgatggaact gctgtaccgt ctcctgcacc
      481 aggcgttgcg cgtccgccac ccggctcatg tccgcctgga tgacggcagc ggccccgcca
      541 ctggcctgaa tccctgcagc cacctcccgg gctttttcct catgcacatg gtaattcacc
      601 accaccgtcg cgccgtcctg cgccaggcgt tcggcgatgg cgcggccgat cccgctggac
      661 gacccggtca caatggccac tttgcccttc aacaactcca tgcccacgcc tccattcccg
      721 tcccgccact cgccatcggc cggctcatcc gcagatcact ctccgtaggc cgatggtctc
      781 gtaaagacca cgtctgatac agtgcgccca tgagcatccg tcttgagcag accgtgccgt
      841 tcggacggtc gctgcgcgaa tatgagttga tgttttcact cagcgcggcc gaccgccgga
      901 gccgcattct ggactgtgcc gccggaccat ccagcgtcaa tgccgaactc acggcggccg
      961 gtggtgacgt ccggtcgatc gacccactgt atcaattcgg cggagcggag atccaacggc
     1021 aattcttctc caccctggat cacgtcattg agcaggtgcg ggcgacgccg cagaattggg
     1081 tctggagtta tcatcgcgac ccggaggatc tacgacggaa ccggatcgcg gtgatggaac
     1141 aatttgtcgc cgattacagc ggggggacgg gaacaggtcg atatctctgc ggcgccttgc
     1201 cgaacctccc gttcgaaacc gaccggttcg acctcgcact gtcgtcgcac tttctctttc
     1261 tctactcgga ccacttcgat gcggcgtttc acctcgctgc cattcgagcg ctgctgcgcg
     1321 tcgcgcggga agtgagaatt ttccctctgc tcgcgctccg cgctgagcgg tcacagcacc
     1381 tggagccggt gtgtgagcaa ctccgtcaag agggccatca cctctcgatc gaacgggtgg
     1441 ggtatgagct tcaacgcggc ggcaacgaga tgctgcgcct acggcgagcg taaccgggga
     1501 ccatcacctg gtcggccact cgggcgggcc attccacctt ccgtgagaca cgaactcggt
     1561 cacagccttc cgctcccgcg gtttcaattc ccgctcgcgc agatagtccg gcaacgatgc
     1621 cgggtcgccc gggtatccga cggcgatcat cgccaccggt tcatacccgg cgggaatgcc
     1681 gagatcggtg cgggccttct ctatctcaaa tcccgccatt tggtgagtca ccaatcccag
     1741 cgccgtcgct tgcaagcaga ggctgagcgc ggccatcccc gtgtcgtgcc aggcatgccg
     1801 gtttggtgtt ccttcgtcct caaaattcag ccgtgcgacg gacaatatca agaccggggc
     1861 gcgaaacgcc cacttcctgt tgccctcctt gaggcaggcc acgagacggt cgtgcgcgga
     1921 aggatccgct ttcgttccga caatgaagtg ccagggctgc tcgttattcg acgacggcgc
     1981 ccacctcgcc gcctcgaaca ggctctgcag ttgttccggc tccaccatcc ggtccgcaaa
     2041 cgcgcgcgga ctccatcgcc gctgtaacag agcatgaatg gggtgggtca catcagcagg
     2101 tttttccatg aacgaatcct tctccagatc ggaatttaca ccaacgttcg cacaatgcgg
     2161 gctggattgc cgacgacaag gctatccgga ggcacgttgt gcacgaccac gctgcctgct
     2221 cccacgatgc tgcgatctcc gatcgtgacg cccggcagca ccagcgcccc gccgccgatc
     2281 cacacatcgt tgccgatccg aatcgcgctt gcggattcca ggccggagcg acgcaccttg
     2341 gcatcgatgg gatgttgcgc cgtatagagt tgcacggcag ggccgatctg tacatcatct
     2401 ccaatggcga taggagcaca gtcaagaaag atgcagtgat aattgatgaa gacgttccgg
     2461 ccgaggctga tgttataccc gtagtcgcac gtgaaggtgg gaaggaccac ggttccttcg
     2521 ccgacggcac ttaggaaatg tcgaagcagg gttgtacgca tatcgtgacc actgtccggc
     2581 aacccgttgt acctggccag tcgctgctgg gcatggcgtc gctcatcgag caactcttta
     2641 tcgcttgcac gatagagctc ccctcccagc atcttgtttt tttcggtctt ctcggccatc
     2701 aactcgacgc tttcgacaag ttacacgggt ggttcaggcc gtacgccaca ttcgtccagg
     2761 tggcgtaaga ggtccgccgg atcatcatag gtacgatagg cgcccgcccg ttccagttct
     2821 tccctcccat aacctcccga caacagcccc accccgagcg cggaagcacg acgggccgcc
     2881 aagagatccc acacgctgtc accgacgacg atgcacctgg cgatgggcat atggagctgc
     2941 tccgcggcag ccagaaacaa atcaggatcg ggtttcgcgc gcttcacctg atcacgggtc
     3001 accacgggag ttccgggagg aagcttcagc agtttcaacg tatgttgcgc actttgcagt
     3061 cgcccgctgg tggcgatggc atagggcaca tgagccgccg tcaatgcgtt caataattct
     3121 tgcgccccgg gcagcactcg gagtgatggg gcctgtttcg caaaggcgtc ggcatgcacc
     3181 cgctgaatgc gctccgcctc gtcttttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnntg tttcgcaaag gcgtcggcat gcacccgctg aatgcgctcc gcctcgtctt
     3361 ttgaaacggg ccgtccggtt tcccgccaga gcgcatgtaa catgaggcca ccgctcatgc
     3421 cgatttgacg gtgaatcctc caaaccggca gttcaattcc tgccgcttgg gtggcctcac
     3481 gccaagccag cacatgctgg taaacactat ccacgagcgt gccgtccaga tcgaagagaa
     3541 acgctacaga gtcaggatcc tgcgaaacgg tcatgaggtc tagttctcca acttcgcatc
     3601 catggtgatc gtggcgttga gcagccgtga aataggacag ccggccttgg ccgccgctgt
     3661 cgctttctcc caactggcgg catcggcctt gggcaccttg cccgtgacag acaaatgcac
     3721 gcccgtgacg gtccagcccg cctctacctt ttccatcgtg acggtggcag tggtcgccag
     3781 ttgctcagcg accagattcg ccgcgcccaa ctgtccggaa agggccatcg taaaacagcc
     3841 ggcgtgggcc gccgcgatca gctcttccgg gttcgtccct gttccattct caaatctcgt
     3901 actgaacgaa tattgcgtct cgctcaagac accactgtcc gtcgagaccg tccctttgcc
     3961 ggttttgaga tctccttgcc attttgctga agcattccgt ttcatgcgat cctcctatcg
     4021 tgaatcgttg atggtcaatg ccgcgcgacc acaggcctcg ccgatcgggt caatcggcta
     4081 ggcactttcc ctggttgcct ttctccttat ccggatcgac gcctcgattc ctgccatgcg
     4141 ccccagccgg atcctgtccg atgggcgacg tagaggtggt tcgaaatgat ctcttggatc
     4201 ggcccatgac tcaactcaat ggtcattggt tcgccggtcc ggagatcgac ggtcacctgt
     4261 cccggctcgt gatcgatcgt cacagagacg ctccgacctc tccattgcag cgggaacgac
     4321 aaccgatccc agttcgatgg aaggtgcggg tcgatgacaa gcccatcttc tcgaagcctg
     4381 acacccccca tgccgaagac agcggcctgc cagagccccc cgagggtagc gatgtgaact
     4441 cctcccgccg cattacccct gtgattggaa agatcgatcg ccgcagcgtc ccgaaaataa
     4501 cgcgcggcca ggtcggaatc gccgagcctg gcgctcacta aggcatgaag ggcgggactt
     4561 aaggagctgc catgtgcggt acggggttca taataacgaa agttcgcctc atgcactgca
     4621 gacgggaatc gttcccagag gagcgcgctc agcgcgacca cgtccgcctg cttgaccact
     4681 ttggactttt gcgtcctgtc gcgcccgagc gtcacatcga tgggaacagg cagcgtgcgg
     4741 tacgcctgga gttcgacctc ctccagttga aagaacccgg aaaattgctc gaacagattg
     4801 gtcggccggt cgaatccggt ggtcatgagc tccgcggtcc gacgccagga atccggttca
     4861 tcgggcttga cctgcaagcg ctcgcatatg atgggccaga catcggaacg gcgcgagcgg
     4921 agccagtcga ccgtgtccgc ggcccgctcc aggttccatt gcgccatgac attggtgtac
     4981 gcattgtcat ccacggattc atggtattca tccggcccga tgaccgttcg aatgtgatac
     5041 tgcccgtccg gctctaaccg gccacgactc gcccagaatc gcgcggtctc gatgagaatg
     5101 tccgcacctg ccgcgagaaa aaaggcctca tccgccgtcg cctgccagta ttgccagacc
     5161 gcataggcga catcggcgct gatatggtgc tcctgaatcc ctgtgagcac ttgaaccacc
     5221 cggccatccg gcgcgatgac cgtatccggc gtgacatctt ctccggtatc ggcggactcc
     5281 caggcgaaca acgctccctg atatcccagc cgccgcgccc tgtcgcgggc agccggaaga
     5341 gtatggaaac ggtactgcaa gagagcccgc gccgccggag gatcggtaaa agtgtaaaac
     5401 ggcagcagat agatttccgt atcccagaag acgtgccctt tgtaggtgga gccggtgaga
     5461 gccctggctc cgatggacgc atactcgtcc ttcggattca cggcgctatt gagatgatag
     5521 agggcaaagc ggagcgcgcg ctgcgccatc tcatcgccgg ctacctccac tccgctggcg
     5581 cgccaacgat cctcccaggc ttccgcatgt tgcagcatgt gggcattcag actcgcctcg
     5641 gagactcgcc gcacatgttc tccggctgcg gcagcgggat cccctacctc gcgggatgaa
     5701 tagacgacaa ccgagcgatc gaaccgggcc gcctcgcccc tttgggcctg ccacgtccac
     5761 tgctcagcaa atccttcgac cgggctattg cccgctccct cctgactgtt ctcatgtcgc
     5821 agcttgttat gagaggccat ggcgatcgtc gttcccctgt ccgtccgcct cacgatcacg
     5881 gctgcctcgc cctccaccct ggaagaccat tgtgtccccg gctgaggccc ttcagtgaaa
     5941 gattcgatcg agatggtacc ggaataattc tctggcgtga tcgtgaccga ttggagcagg
     6001 aggtgtcgat cgtgatgaca cacggcgcgc acgcaggtga gggccgtcac tcggccctgc
     6061 tcatcctcgt gccgccaggt tcgaaaaagc agaccatgct gcaaatcgag gatgcgtcgg
     6121 tgatcgagcg tggttccggt ttcgagcgac agcgggaccc cttccaccag cacgcgaagc
     6181 tgcgtccaat cctggagctt cgtgagctcc agggtacctc ctggggcctc agaggcctca
     6241 aacgctccgg ccaggaaggt ggcaggggag gagaggctcg tgcgttcggc gagcgaaccc
     6301 cgcgtcccca catagccgtt cgccatcgta aagatcgact cgatctcatg ctcccacccc
     6361 ggctcgaacc ctgattcgac cagaacccag cttgggtcaa tggcgaaggg cagattggcc
     6421 gacaataacg ggggagccgg tttcgcctct tcttctcctt gatccggtcc tgacaggatc
     6481 gatttgtcgc gaggtatcca catcttgacg ttccttcaca tctccgaggg gcagaatggt
     6541 agcatgcaga ggccatcacc tatcccgtat gccgacgcac agactgacac gcaagggaga
     6601 tcgaggatga cacaaggcgc aaccggagaa caaccgttcc atccacggtc cattgatctg
     6661 accaagttcg atgcggtgct cttcgatctt gacggagtcc tcacaaaaac cgccgtcgtg
     6721 catgcagccg cctggaaacg cttattcgat gagtacctgc aggcgaaagc ttcccgcgaa
     6781 caggtccctt ttcgaccctt tgatgtcatg ctcgactatc agcgatacgt tgacggcaag
     6841 ctccggtacg acggggtacg ggcctttctc gaatcacgca acattgtctt gccctatggc
     6901 accgcccacg atggcccgga gcaggaaacg gtccaggggc tcggcaacaa gaaggacggg
     6961 tacttccaag ctcatctcaa acagcatggc atcgagctgt atgacgacgc ggtgcgattt
     7021 cttcggaccc ttcgagcaca gggggtgcgg acagcggtcg tctcagccag caagcacacc
     7081 gcggccgtcc tacaaaagac cggcctcgcg gactacttcg atactcgagt tgacggcatc
     7141 gaaagcgaac gactccatct tgcggggaag ccggcaccgg atggtttcct cgaagcggca
     7201 cggcgactgc aggtggatcc accgcgagcc attgtcgtgg aagacgccgt tgcgggcgtg
     7261 caggccgggc gcaacggcgg atttggattg gtcatcgggg tcgatcggac cggccatgcg
     7321 gaggagctca cacgaaacgg tgcgcatctc gtgacgtccg acttggctga actgtgcgta
     7381 tagctcctga catcgccgcg tccgactgga tcaacagatc tgatgaccgc acctcgactc
     7441 gcaggtcccg aacaaatcca ttccgattcg gcatctgcag aatgggggcg gggaggccga
     7501 gacctccccg ctccgagctg ccgttattgc acctctaccg tgatcgtatc ggtcgcggcc
     7561 aaatggtcgt ggtcatgggt tgcggcttgg accttgatct catgtttgcc cttgttgaga
     7621 cctttgaatg tgccgggaaa cttcttctgc ggttcgccgt ccagatacac atgcccatga
     7681 gccgccttgg aacctttggt gagttcatag gtgagctcga aggtctctcc cacttgtgcc
     7741 ccgtctttcg gcgaggtgat tgtgatgtgt gatccgtcat cgacctcggc gaacagggga
     7801 ccggccgaaa aaatcaggcc ggccgtgaac aacgtggcga aaaatccctt catgtgtcgc
     7861 atcgcaccct ccttggttgg gctctggcga tcgtccattg accgctcagt attcatcacc
     7921 agcgcgcttg tccctcgatg gtgatcgtca gttcgactca tcgcttagtg ctctccgtag
     7981 cgttccatcc acttgcgaag gtgctccacg gcttctgcct tctgatcctg ccgcagggtc
     8041 gcatgccact cggccacctt cggcaacacg cgctgcatca agcgggtctg tcctgcctgg
     8101 tgccgctcaa acaattgggc caccttggac tgatccaacc gatcactctg gacctgcgcg
     8161 atcacatcct ccatgatgac ccggtgctct tgttgtgact cggcccgggc agcgatcact
     8221 tcgtctttca cgacggccag cttcgccttc tgctggtcat ccaggtccaa gtgtttcgca
     8281 atcttgcccg tcatccagtc agcgcgctcc gacggcgtat ggtgacgatg gcacccggca
     8341 ccgacaagcg ctccggccag gaccagcgtt ccgacgacca ccctgaatgt gttttgcata
     8401 ctcatacggc cctccctttg gtgtgatctc acgcactcag tactcgaagg cgacgcgata
     8461 gagcgccgga gtcatttcaa cccccagttc cccgagcttg gccttcatcg gcgtgacgct
     8521 cttttcgtag gccttccagt acacctcttc gttctcccag atcgctacgt tgatgtagtt
     8581 aaatttgctg tccggcttga tgcttgtatg gaattttccg ctgatgaacc cctcctgctt
     8641 cgtgatgtgc tccttgacct cctgccacca ctgcacaaat tccgcttctt tggtatgagg
     8701 gactgaaaac acattgatca acgtaaccgg catggcatgc ctccttgaat atgttcgatt
     8761 ttgacttcca ccttcatctg acacttgttc agacgcaggg tttgatcgcg acccacctgc
     8821 ggcctggcgg gagcagggca cccgccagac ccattcaggt tattgcgcct tccgcagatc
     8881 gatcacagtt ccttcttcgt tcagctcgac gacaatattg tctccgtcgg cgatcggctt
     8941 ggtcttgatt tccatccgct ccagcgggaa cactttctcc ccctccggcg tggcgagctt
     9001 gatctgattc ttgttgggcc ccgtacggat caacttgccg aagatcagcc gatgggtccc
     9061 ggccttggcc ttatcttttc catgcacatc gatcaccatg ccctcttcat tgatccagag
     9121 ggacacgtga tcgccgaccg ccgcatcggc cggggcatac ttcttcaaga gcgtgtactg
     9181 tccggccggc gtcttcacgt aggccaggct cgcatcaaac ttgacgaccg tgccgtccac
     9241 ctgaatgaca taaccgggct tcgtgcgcgg gtgctcgtca aagctcatct ctaccgtgaa
     9301 gcgatggatg tcgatcatct ttcccgcttc gttcaactcg atggtcaccg gtgccccttc
     9361 ttccaaggcg gacaacttgg actttccggt ttggacatcg atggccttct ctccttccgg
     9421 cgtccacact ttgatttctt tcttatccgg cgaggtatag gccaggttgc cggtcacgaa
     9481 gcgatgggaa tgggccttgt ctcccttgcg atggacatcg atcacggcat tgttttcatt
     9541 gacctgcatg tccacttctt ccccgacctc aaggttcttg ggcgtgagcg tcgacgcgat
     9601 tttcatcgtc ccccaggggg tcttcacggt ggtgatgcct gactgcacat tcgacacgac
     9661 cccgctgacc ttcatgtgcg tggccttctt gcccccgcct tcggcggcga atccataggg
     9721 caccgccatc gacaagctga gaaccattga accgagaata accattgtgc gtgtcatcgt
     9781 ttgactcctt tcatccgtga ttgtgaacgc ctgtcttgcc taaattctgt acgatggccc
     9841 tggtcaggca gcctgatgcc tgaatcgtga cccccatgcg gtacatctgg gacagaagcg
     9901 tcgtgcccgc ctcatcgata aaggtcactt cccggagatc gacgtggcat gggaccggcc
     9961 cgtcgaactc ctgcttcaac caacaatccc gcagctcacg gacccaggga cccgccaacc
    10021 gcccggaaag aacgaaggtg gttcccgggt tcgtattcga caccgtaatc ttcagcatga
    10081 tcgtggaccc cttcgtccct tgagtccggc cgggccgatg cccggcccca ccatgacgct
    10141 cagaacgtgc gcagagcgaa actcgcttcc cgcgccttga gagccgggga ttcctcacca
    10201 agccaccggg cgattacgcc gtccagcgac cacttgccgc caccgacgac cagcagcaca
    10261 aggctcatgg cgatcaccag gagatggaac tcaaaaccct caccttgctg ctgcccgaac
    10321 cagttcatga agaacccctg cggcaagtgc accgtcatga tcgcgccgag cataatcaca
    10381 atgaagctgg ccgccgtgaa gcgcgtcagc aatccggcaa tcagggccag actcccgatg
    10441 gattcgccga tgatgaccag gaacgccacg atccatggca agtgcatgac attggtaaag
    10501 aagcccatcg tgccgtcgaa gccattccca ccgaaccagc ccaacaattt ttgggcaccg
    10561 tgggcgaaga tcactccacc caacaccact cgaagaatca aacccgccca ggactcatcc
    10621 gttctgaaaa agctgtacat gtcacacctc cttgatatga attaccgtcg gtggctccgc
    10681 acccattgcg gaaccgcact cgccattcga tctttcagga acaaccctgc cacgaagagc
    10741 ggcagggtca cgagcattcc gtaccaccat ccccacagcg tcatgtcttt catggcctca
    10801 cctcctaatc cctcatgcag cgctacttca ggtaatggca aaggtcattc cacctcgctg
    10861 cctgtgctgt cgatatctgt aaccaattga aacaacgggc aaaccttgtc gatcaccaac
    10921 aggccgcatc cgcatgagtc attgagtgac gatgcgacgg tgacgagggt tcgccaggtg
    10981 acgaaggaga tcgtcaggga gggcagagaa caggtcgagg attacgcttc gggatttttg
    11041 gggtcgtagg ttgtcgcgcc tctgcgccat gggacccatg gcgcagagac ggttggtcgg
    11101 tcggctagag gatggggctc atcgatcgct gaactcgaag atgtcgtgcg cgtcggtcac
    11161 gacatgcatc ccggtaatgc cggcagattg aatatcccca ctcgccagat gcaaccggtg
    11221 gcccacaacc gcaccggcca agccgtgcct gggcatcggc atcatcggaa gctcggacca
    11281 gctgttcgtc gccggatcgt acgcttccaa tgcgcggaat gcggccatga gatgtccgtc
    11341 ctgatgttct ccaccagcca cgtagatgcg cccgttgtag accccccagg cacctccgct
    11401 ccgctcggtg ggcatgcggg ccctgaccag accccactga tcggtcgccg gatcgtattc
    11461 ctcgaccaca ttggtgttgc tggcggtgaa aatgaaggcg cttcccaggc ggccgccgat
    11521 gacgtagatc ttgttgttta cggcaccgac ggcggcgtgg ttgcgggctg tcggcataga
    11581 cgagcgttcg gaccagctgt tggtcttggg atcatattct tctacggtgc ccaccgaccg
    11641 atggggtcgc gtcggataga gagcggcttc cttggaacca ggatgcatcg ctgcgccacc
    11701 gatcacatag atcttcccgt tgaccgtcgc cgcaacagct gacccgcgtg ctgatggcat
    11761 ggccgccaag gccttccaac tgtcgtgggc cgggtcatac tcccaggcgt tgttgatcgg
    11821 cacccacgcc ggaggtcccg actgtggcgg cacaaagccg ccgaaggcgt agaactttcc
    11881 gttgagttca gtgaacgcca catgatgcgc cggcagcgcc atcggcttgt tcttggtcca
    11941 tttgttagcc gacggatcgt attcgtacac aaggccttgg gggatccagc cgggcccgag
    12001 tccaccgaac acgtagaatt ttccgccggt cgacaggccg accaactctt cggacggctc
    12061 cgggaacggc gcacctttgc tccacgcacc cggcgtgtcc gcctgggcag gagcgaccat
    12121 catgagcaga ctcaccatga ccatgaccaa tagcgtgtgc ataagatgcc tccatgaagg
    12181 gcagaccgag cgatggatga accacagctg ccctgttgtc ttccagtggt tggtgattgt
    12241 gttgcgctct cgaccgttcg ccccctctgt gatcggtcta tgcttcacgt ccatccgatc
    12301 catcgcgccc gactgtcaga aagtggcacg cgggtggcgg gggtattggc tcattggaat
    12361 tgccgtcgga atcgccacat tccaaacccg aacaacgctc ctcccaatag gagcatggcg
    12421 acgacgggct tccagagcag gtccaggcct gctcccttca gcatcacgcc gtaggtgatg
    12481 tcgatgtagt accgcaaagg ggagaacgtc atgaccgtct gcacccactg aggcatcgat
    12541 tcgtacggcg aggtgaggcc cgacaacaac agcatcgggc ccacgacgaa gagcgtcatc
    12601 atgcccactt gcgcctggtt cttcgcaatg gtcgccgcca ccaatccgaa tccggccgtc
    12661 gtgaacacat gcaaggtggt cagcagaaaa aagagcccgg tcgaaccttt catcggcacg
    12721 tgaaagacgg ggtgcaacac gccatacagg gcgagactga gcgcgatgag gatgaccccg
    12781 gacatcgcca gcaccttcga aaacatgatc tgaaaggggg acagcggcga caccagcaac
    12841 tgttccaccg tgcctcgttc tttttcacgg accagcgcag cagccggcaa caacaccgca
    12901 aagatggtcg tcatgcgtaa cacatgtgcg atggactgaa accatcgttc atcctgggta
    12961 ggattgaacc agacgcgatg ggcgctggag acccgtggta tggctccctc tgctccggaa
    13021 agacccgccg aggccaagcc cgtctcggtc ccgaacaggc cggcgattcg cacgctatac
    13081 ccggctgccg agagaccctg gggtgcattg gtggtatcga cgaggagttg caccgccgtc
    13141 cgttccccgc tcatcaaggc ctcgtgaaat cgtggcggaa tctccaacag caccatcacc
    13201 ctgccctggt ccagttgccg gagcccctcg cgaggatcgc tgatctcccc ggcgaatttg
    13261 aaataggggg gctgaaaccg atggatcagc tcgcgggacg agtgactatg atccgcatcg
    13321 tgcaccagca actccgcatt ggtcagctgc atcgtgatcc cggctccgct gaccaccacc
    13381 gagagtgaaa acgaatacaa cagaaacaac aacagcggca cgtcgcgcga cagttgcagg
    13441 atttccttcc acgtcatcgc cgcgagccgc cgcccccacg tgactgcctg ctgcctggcc
    13501 gtcatgcctc acccctcatg tcttcggcct cttggtgaag agccgatacg taagcccgct
    13561 caacgcgacg gcaaacatcg ccagcgccag caggtcggtc cacagcacgt ccgcgcccac
    13621 acccttcaga aaacttccgc gcacgatgtt ggtgtaatac atggcggggt acagatgcgc
    13681 ctgcaccttc gcgcctcgag ttaacgaggc caccggcacc aagagaccgg aaaagagaat
    13741 ggtcgggatc atcgccacga ccatcgtgat aatcagcgcc gccatttgcg tctgcaccag
    13801 cagagaaatc agcaggccga tccccgtagt acagcacacg aacagaactg acgccgaaaa
    13861 gaacaggagg aagcttccct taaagggcac ctgaaagagg ccgacggcca tcagccataa
    13921 cacgcagacg ttcacgagtg agatgacgat atagggaagc agcttgccgg tgaggaattc
    13981 ggcgcggctc acggtggagc tatagatatt gtaaatcgaa cccgtttctt tctcccgcac
    14041 gacccccagc gccgtcagca ggggcgaggc gagcatcagc gtgaacatga ccagcgccgg
    14101 caccatggac caggtactcc ggacttcttc gttgtacaga tagcgcacct cgacggtcag
    14161 cggacgcacc agcgcatcgg cctgctcctg ggtgagacca cgggttcggc gcaggtactc
    14221 gatcagtcgc tcctcggtga acgcctggtt gatggcaatg acatagcctt tggcgatatc
    14281 cgtatggaga ggaaaggtcc cgtcgagcaa ggtctgcacg gaggccggtt cccccgccgc
    14341 caactgctcc tggaaccgct cgggaacgat gatggccgcc cggatcttcg tctcggtcaa
    14401 caggcgatca agggcacgtt catcgtcggc atacccctga aatgaaaaat agcgcgattg
    14461 catgaaccgg tggagataat cccgactcaa ctcgctccga tcccgatcca ggaccgcgaa
    14521 cggaatgttt tcgacatcca gattcaaccc gtacccgaat accaccaacc acagggccgg
    14581 caacaaaaag gccaacagca agaacagtcg gtctctggtg gtctccttcc attctttgag
    14641 cgccacggcg ctgattcgtt gcagattcag gctgacacct ccttttgcgc cgcctgttcc
    14701 agtgccatca cccgtgacac aaagacgtcc tccaagctga gcatccgcgt ccgcaccgct
    14761 tcgaccgcaa tcccggagcg ggccaggaca tcccgaagac gttcctcgtc gcgacccgga
    14821 tcacgcgaga gcacatggat cttggtgccg aagagcgccg cgccggcgaa cccggctgcc
    14881 actacgtgcg cgagtgcgat ccccggcttg tcgacgacca tctccacgag ttgcccgatg
    14941 tcccgctcga cctgtttttt caggtgcgcc ggcgtcccct cggccacgat gcgtccggca
    15001 tacatgaggg ccagccgatc gcaatgctct gcttcgctga ggtagtgcgt ggtaatgaga
    15061 atcgcgactc cttcctcgcg ggccaaccgt gacagcagct cccagaaccg ccgacggccg
    15121 atggggtcca ctcctgacgt cggttcatcc aaaaacaaca cgcgtgggct gtgaacgagt
    15181 gcgcaaccca acgccagacg ttgccgcaca cccatgggaa ggcgccccgt ccgatcatcc
    15241 tcgtagccgg tcaggccggc catctccacg atccactcca tccgttgctg cgcctctcgc
    15301 cgagccaacc cgtagatacc ggcaaagagt cggatattct cgatgacggt caggtccaga
    15361 tagagggaga acgcttgcga catgtagccg atgcgctcct tgatcgcgcc gcccgccgtg
    15421 cgcatatcca cgccggccac acgcccttct ccggccgtcg gaggcacaat cccgttcagc
    15481 atcttgatga cggtcgtctt gcccgcgccg ttcgcgccca gcaacccgaa gatttcaccc
    15541 tgcttcatgt cgaagctgac ccgatccacg gctcgaaacg aaccgaagtc ccgaatgagc
    15601 tccttggcct cgacggcaag tccgtcgagc tgctgcctac ccgcggggcg agcggactgg
    15661 tccgatcctg ccgactctcc cggctgttct tctcgctcct tcaagagcag cgccaccacg
    15721 acatcttcca actccggctc ctccgtgtgg atgtgctcaa ccggtaaatt ttccagcgcc
    15781 tgccggatcc ccgcgacggc ggcttccggc tcccgtnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15901 nnnnnnnnnn nnnnnncggt aaattttcca gcgcctgccg gatccccgcg acggcggctt
    15961 ccggctcccg ttctggtgtg aacacgtgca gcaaggggcc cagggattcg acttgggcat
    16021 accggcgttt caggcgggca accgcttcca gttgcgggct cgattcgaag gtgacgacaa
    16081 ggcccgggac cagtgattgg acttctgcag gagtgcccga tgccagcacc cgtccatgag
    16141 agagaaacga gagccggtga aagcgggccg cttcgtccat gtaggcggtc gacaccagcg
    16201 cggtcatgcc cttgtccgcc tgccattcgg cgagaatggc ccagaagtcc cgccgggaga
    16261 caggatcgac accggtcgtc ggctcgtcca gaatggccag ctccggctca tggatcagcg
    16321 tacagatcaa gccgagcttt tgcttcattc cgcccgagag ctgtttcatc agccggctgc
    16381 ggaagcgatc caggcgggtg atcgccaaca gccgggactt gcgcgcgcga agctcaggct
    16441 ccgggaccag ccgcagcctg gcaaagaagt cgatgttttc ttcaacggag agctcagggt
    16501 agaggttcag ccccagtcct tgcgggagaa atccgatccg cggtttcacc cgttccgccg
    16561 cccgctcgga atccacgagg gtcccgaaca cttcggcgga tcccccgtcg tatcgcaaca
    16621 ccccggcgat cgctttcatc acactgctct tgcccgcacc atccggtccg atgaggccat
    16681 agatttcacc cctcttcacg gtgaggtcca ggcggtcgac cgcaagatgc tgcttgtaac
    16741 acttgacgaa gccggacaca cggacaacca cctccggtga cgatgcagcg ggcttcgtca
    16801 tcgaggtttc gaccaggcca cgtcgtcctt ccagcggatc accgcgtccg ccggcagacc
    16861 cggcgtcagc cgatggtcag gattctcggt gagatagagc cgaacggcat agatcagctt
    16921 gacccgttcg tccggagtct ggacttcttt cggcgtgaat tccgctttcg aagcgatgta
    16981 ccggaccgtc gcctcgaacg accggtcgga aaacgcgtcc gtatggatgc gggcgggcaa
    17041 atcgaggcgg actttaccga tttggagttc cggaacatag acctgcaggt agaggcggtc
    17101 gagatccacc aattcgagca ggggcgcgcc ggttgccacg acctctccga cgtccaccat
    17161 ccgagtggtc accgtgccgt tcgtcggagc cacgatcgtc agatcgtcga gtatgctacg
    17221 ggcctccgcg aacgtcgcat ctgcttggtc gcgttgccgt tcgagcgccg cgacctccgc
    17281 ttctttcgat cgtattcgtc cccaccccaa ttcggcctgc gcgagttcct tgcttgcttg
    17341 gtcaagggcg gctttcgcca cggcaatctc cgtgacagcc accttccagc gcgcctcagc
    17401 ctgatccacc tgctgctgcg acaccgcctg ctcgggaagc agggctcgga tgcggtcggt
    17461 gtcccggcgg gcttcatatt cgaccgcctg tgccttccgg atgacggcgc gcatgcttgc
    17521 gactttggcg gccactgctt cgatgctcag aggcacttcg aggttcagga ccgcaagcgc
    17581 ggtatgggcc gcttccacct gcgccatcat gctgtccacc agccgtgccg cctgatcgac
    17641 cttcgctctc gtttgggcgt cgtccagccg aatcagcacg tggcctttgt gaacctggct
    17701 cccttcgcgc gccgccaggt ctacgatgcg cccgggaaac ttgctggaaa cggtgacgtg
    17761 gtctccttca atccgcccat tggcctggat cagtccctcc gggaggccgt cctgccacac
    17821 cagccgatcg aggatcacgt atccggcgat cagcaccgac gcaaggacgg ccgccgcaat
    17881 ggtcgaggtc cgaagggtca cgactcagtg gtctccgtac cggtccatcc acttgcgaag
    17941 gtgctccacg gcttcggcct tctgttcagg ccgcaaggtc gcatgccatt ccgccaattt
    18001 gggcaacacg cgctgcatca ggcgggtctg tcccgcctgg tgctgatcaa acagccgggc
    18061 cagcttggcc tgatccagcc ggtcactctg gacctgagcg atgacctctt ccatggtcgc
    18121 cttgcgctcc ttctccgact ccgcgcgtac ggccatcgct tcgtccttca cggcggccag
    18181 cttggccttc tgttgttcat ccaggtccag atgtttagcg atcttgcccg tcatccagtc
    18241 ggctcgctcc gtcggcgtat ggtgtcggtg acaaccggca ccgacaagcg ccccggcaat
    18301 gaccaacgtc ccgatcgcca ccctgaccgt gctctgcatc ttcatgctgc cctccttggt
    18361 gattggtgtg atatacgagc gactcttccc ctcacattga atcaggagtc ggaggggaca
    18421 tggtagggac ccccaccatt attctctagg gcgagagagg cccagcttct tgattttcga
    18481 ggtgagtgtc gtacgcttca ggcccaagcg agcggccgcc ccatccggtc cgccgatgac
    18541 ccaatgcgtg tcgcgcaaga cgcgcaggac ctgctcgcgt tccgcatcct gcagcgtagg
    18601 ggtaggcgcc ggagcctgcc gcgcctcaat tttgagttcc aagagcggca cctgcaagtg
    18661 ggttccctgg gtcagaatca ccgcgcgctc gaccagattc tccagttccc ggatgtttcc
    18721 cggccagtga tagcgggaca gagcctccaa cgacggcccg ggaatcgttt cgatgtgctt
    18781 cttcatgcgg gcagcataat gctgcgtgaa atagcgcacg agcaccggaa tatcctctcg
    18841 acgctcgcga agcggcggca gggtaatcgg aaacacgttc agccggtagt ataaatcgct
    18901 tcggaactca tggtcttcca ccattttggc cagatcccgg ttcgtggccg cgatcagcct
    18961 gatatccacg tggatggtgc gtgtgctgcc gagccgctcg aattcctgtt cctggaggac
    19021 acggagcagc ttggactgca gctccaacgg gatttctccc acctcatcca gaaagatcgt
    19081 cccgccatgc gccaattcga atcggccgac tttctgggcg atggccccgg tgaaggcacc
    19141 ccgttcatgt ccgaacagct cactttccag caacccggtc ggaatcgcgg cacagttgag
    19201 cttcacgaag gtccgctggc tacggccgct gagccggtga atcgccctgg caatcagttc
    19261 tttccctgta ccggtttcac cctgaatcaa cacggtcgcc tgggtcggcg ccacgatctc
    19321 gacttgcccc agaacctgct tcagcgcgcg actgtcgccg atgatgtcgt cgaacttgtg
    19381 ctcaaggcga atttcttctt ccagatacaa cttctcttcg gtcagtcgat ctttgagttc
    19441 ggtgatctcc tgataggcca gggcattgtc caccgcgagg gcgatctgtc cggccacctg
    19501 actcagaaac tcgacgtcct gttcgccgaa cgcctgctcc tgccgactgg ccagattgag
    19561 gcagccgatc acgcggtctt gcgacaacag gggcaaggag cacatggagt ggaagccttt
    19621 catgaccagg agctgaggca cggcgtgcga aaatgccgtc aggtccggcc gattgttggc
    19681 gaccgcgggg cgcctccgct ccaacgccag gccggccaga gaaccggcaa catcggacac
    19741 cgccccctcg gtcaacgcgc cctggttttc aggaaaatcc aaggcgtgga ggcggacgcg
    19801 gccgttcttg ccgtcgcaca gaatcaggct ggcatactct tgcggcacca tctcgcgcag
    19861 acagagactc actgcgctga atacctcccg cagatcgagt ttggacacca tcgtattggt
    19921 catgcgaagc agcagactga atcgatcccg ctgccgttcc acatcttgcc tggccgccgc
    19981 cgccgcttcg cggttcagca cattttccac ggccaccgcc acctgccgac cgatctgttg
    20041 catcagtgca agatcagaga ctctgtacgc atctttcttc agacttgaaa atgccaacgc
    20101 gcccaattgg cgaacggcgg aggtcaaggg caccagacag caggactgca cgccatcctc
    20161 gcgcatcaac gcgatgagtt tcggccaccg atgttcttcg gccagatcgc tcaggatcac
    20221 cggctgctgg gtctgccaca ccagaccggc cggcgtgtcg gcgggatggg attcctgacc
    20281 gccgataatg tcggccggga cgttcgctcg caacacatga agccgcatca tgtcgcgttg
    20341 cgcatcgtag agggagaggc cgacaaagtt gacgggcacg agcagcggca gcaggcgggc
    20401 gagatcctgc accaacccct gcaagtcagc atggctcgca atggcttcca ccacctgcag
    20461 cagagcgcca tatcgc
//
LOCUS       DAOQRM010000028        20432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1802_length_20432_cov_14.504981, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000028 DAOQRM010000000
VERSION     DAOQRM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20432
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1802_length_20432_cov_14.504981"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="PKK81_04225"
     CDS             complement(<1..1220)
                     /locus_tag="PKK81_04225"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNV32106.1"
                     /translation="MASTKHIPIDQLTVGMFIAGLDQPWYRTPFLLHKWLVSNPDDIV
                     QLKRHGILVVTIDTDRGLDVGAAAPPAPATAPPPDEPPPVSVEAAVPQESAQVPQAAV
                     AAAAYRESMAAVERVFSDLEAGNPPKIAALKPIVSRLLEQIVAQPEAMLIQFCLDKVR
                     RFDATLANHGMDVCVLTLILAVENGCAEADLESLGLGALLHDIGYVRLPRNLYRKTTP
                     LTDQEQILMKQHPQLAATVLTQVGSIPDAVSRIILQHHEYQDGSGFPQHLKQEAVSML
                     AQLVGLADTYDDLVSARHGRPPLLPHDAIRQLFVLGAKGRYEKTLVEVAIKALGVYPL
                     GSLIKLNTNECALVVGLNHEDRLRPRIRIIKGAEGHSQQEPFEVDLQNQPPDQPTRTI
                     LRALDPKLEQVDLP"
     gene            1434..2147
                     /locus_tag="PKK81_04230"
     CDS             1434..2147
                     /locus_tag="PKK81_04230"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNV32107.1"
                     /translation="MSELVICRHPSSFLSVGLSLQLSVPKDETQGQYGSSVLGWKHRS
                     WIVCEWPFHLGQPIPCVKGTLCLLRYIYEGKMIGYRAEVLETQMQPFPFLLLAFPSEL
                     EEVPLRKHGRVSAQEPIVLSAVQEYASSSRNEPPIRIGGLLKDLSASGCAVLIQRPVQ
                     DFYLGMALRVEFEIIGTGHVNNLTGVVRNLSLQADGTHLGLEFRFDGKETIEYRGWGG
                     SVRNALESFLHRKHSFESA"
     gene            2344..3537
                     /locus_tag="PKK81_04235"
     CDS             2344..3537
                     /locus_tag="PKK81_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV32108.1"
                     /translation="MTLHAQSAKLNPRWCLAQDTIDRQDIDHLIEWLRTYPRLTKGAV
                     TLDFERQWSEWLGRPYSVHCNSGSSANLLMYYALLRSGKLRNTTVIVPSVGWVTSIAP
                     AIQFGFTPIMCEADPDTFGLDLNHLEDLLQRHDAQTVLLVQVLGVPHRMQELQTLKDR
                     YGFYLLEDACAAIGAEYGGKKIGTFGDMASFSFYFGHQMSTIEGGMVSTSDKQLADML
                     LMLRSHGWSKDLDQSSHQALLTQYQVDDFHSPFVFYEPGFNLRSTDLNAFIGIEQLHK
                     LDWMTGRRQANHERYLQHLGSRFYTQRPPTGSKVASISFGLLADSTEQRRRIVRALVT
                     EGVETRIFSAGNLGLHPFWMNRYGKASFPVADRIHHCGFFLPNHASMNEQDVAHIARI
                     VLEAA"
     gene            3590..4537
                     /locus_tag="PKK81_04240"
     CDS             3590..4537
                     /locus_tag="PKK81_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV32109.1"
                     /translation="MTTASTHVLVTGGAGYLGSVLSRGLLDRGYRVTVLDNFMYRQNS
                     LMDCCDEEGFQVVRGDCRDERLLTDLLRTVDIIVPLAALVGAPLCDRDRVGAYTVNFE
                     AVQLLCKLSSPRQRIIFPVTNSGYGIGQPGVPCTEESPLRPISLYGETKVKAERVVLD
                     RGNAITLRLATVFGVSPRMRMDLLVNDFVWRAVQDRAVVVFEGHCKRNYIHIRDVVRT
                     FLHAIDHFDEMKDRPYNVGLDDANLSKLELCRVIQRLIPHFVSFEAPIGEDPDKRDYI
                     VSNQRLLATGFKPEWSLERGIRELMKCYTIIKAGQYANV"
     gene            4568..5638
                     /locus_tag="PKK81_04245"
     CDS             4568..5638
                     /locus_tag="PKK81_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HNV32110.1"
                     /translation="MIISRTPFRMSFFGGGTDYPVWFREHGGAVLATTIDKYCYISCR
                     RLPPFFEHRSRIAYSRIELVKNNEEIEHPAVRGVLKYLNINDGLEIHHDGDLPARTGL
                     GSSSSFTVGLLHTLYALQHIMPSKDQLAQAAIHVEQNVLGEAVGCQDQVLAAHGSLCK
                     ATFFQNGEIGHTPIIMQPDRLAAFQSHLQLYFTGFSRIASEVAREQIDRTSQRKAELF
                     AMLEMVEEGITILTGGGDLAAFGTLLHEGWMLKRRLTSRITTPAIDEIYTAARAAGAL
                     GGKLLGAGGGGFMLLFAKPEDHERIRSALPGLLQVPFKFEGLGTQIVFYQEDHLMLDD
                     VWAHNSADTASQLKAALLGKAA"
     gene            5641..6402
                     /locus_tag="PKK81_04250"
     CDS             5641..6402
                     /locus_tag="PKK81_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNV32111.1"
                     /translation="MGRFADCDVVILCGGLGTRLQSVVTDRPKSMTEIHGRPFVACLV
                     EHFLRHGTRRFIFSTGYKGDMIEDWFGRHRGGYETLFVRDPAPLGTGGAVAQAMKLVR
                     SNPFLVLNGDSLCELDPERLLRFHTRKRARATIAVTQADSREDTGAVTLGEDDRVLSM
                     VEKPRTRTTGYHNAGIYLFDRTVEALFPNSRSWSLERELLPQLVTEPFYGFVTASALY
                     DIGTPERLAHFREVWKDPSIHFPVTSMHQEQGSLL"
     gene            6498..7622
                     /locus_tag="PKK81_04255"
     CDS             6498..7622
                     /locus_tag="PKK81_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNV32112.1"
                     /translation="MDRAQALERQGYRIYHLELGNPRMAPPGEIVDGTVQALREKQLG
                     YAPMAGVKELRAALAARYTSLTGEALTDSQVVISPANLLIHQFLDITCNPGDRVVLFT
                     PAFPSYWAAATHQGLQVRPVPLSERDGFHLTRQAIDDALAAGPRAIVVNNANNPTGAL
                     YDSTILRALAARCEEAGIWLLSDETYADLTFDGSFVTLASARAGYVVAMSSFSKVCSI
                     PGFRTGYAFAHEAVAAKLALSNSTLYSCLPLFTQLGCLAGMKVLDTYANEVRSRGSRL
                     IGSCHDRINRSGILHCHKPESGFYLFVDIARTGLDDMTFCRRLLDDHHTAVTPGRSFG
                     EAYASFIRIAVCGQEEDVHEGLSRTIAFAQQLGGCRVQAA"
     gene            7606..9114
                     /locus_tag="PKK81_04260"
     CDS             7606..9114
                     /locus_tag="PKK81_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV32113.1"
                     /translation="MSKPLDVLLITPPSRVQVYQELSRDFAAIEPPVWSGLIATFLRQ
                     HSCSVAILDAEAEGLNHQQTADRIAAIAPRLAVFVIYGQQPSASTQCMPAGRKVCEIL
                     TALADIPTLVMGTHPSALPKRTLLEEPYTYVCQGEGPATILGLVIALRAPQHSLREVP
                     GLWHMEKGVPVGNAPAQLLTNLDCEVPGQAWDLLDMTRYRAHNWHCFGNLEARAPYAS
                     LQTSLGCPFTCSFCCINSPFGTPMLRTWSPDNVIAQIDRLVRDYGVTNIKIPDEMFVL
                     NRRHVIGICDRIIERGYRLNIWAYARVDTVQDEVLAKLARAGFTWLGLGIESGSQHVR
                     DGVEKGRFVERDIVATVDKIRSYGIHVAANYIFGLPDDTMESMRATLDLALTLNTEWA
                     NFYCAMAYPGSPLYDLAKQKHWTLPDDEGGPGWIGYSQHAYECSPLPTDSLTATQVLA
                     FRDRAFMEYFTHPDYLGMLRQTFGAQVATHVEGMCRHQVRRRHHDVVTDAVA"
     gene            9140..10978
                     /locus_tag="PKK81_04265"
     CDS             9140..10978
                     /locus_tag="PKK81_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNV32114.1"
                     /translation="MIKVADFIIHTLAERGIDKMFVVYGAANGDLIDAFTRTAATEYV
                     AVMHEQAGGFAAEAYAKVKGVPGVAIATSGPGGMNLLTAMGNCFYDSVPCVFMTGQIN
                     SRFLRPDPAIRQIGFQETDIVAMAAPVTKYAKMILKPEDVRYELEKALWLCQEGRPGP
                     VLLDIPLDVQKTMIDAKKLIGFEPPAAVSFDLTVVDEQISRFITELQHAERPVILVGG
                     GVRLADAQDDVRALGRRLNVPCFPTWNALDVISSDYENYGGRVGTYGGAGRNFGIQNS
                     DVLLSIGSRISGRITGGNVQSFARQAKKFLVEIDPAMVQRKFQQVPFDVNILCDAKVF
                     TQRLLIALDRRSKPLPNYSGWTERVMEWKRQYDPVRPEFFAQRERVHPYAFMRRLSEK
                     MGATDILVGDCGGNIVVSNHAFETKYGQRNLTNNGNSPMGFSFAGAMGAWFAAPSRQV
                     VCTIGDGGFNMNPQELQTFINYGVKVKTFILNNHIYGITKAYQETNFQGRAEACGPKG
                     YNPPDFLKIVQAYGIKTVAIHNHAEMDAKIEEVLQFDGPVVCDVDMHEFHTYEPRIFG
                     WKTPIEDMYPYLPREEFRANMVIEPAEGWMNPEYPDVVRRNDRSQP"
     gene            10989..>15435
                     /locus_tag="PKK81_04270"
     CDS             10989..>15435
                     /locus_tag="PKK81_04270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV32115.1"
                     /translation="MSQGARETNRVTPLDVEYYQFCREQLRPYFGRVMWASQGLPLRH
                     VVMQELVRLEASRQGAGPFHILEVGSWAGGSAITWAEALTRHHRADGRVVCVDPWKPY
                     FDVKKRPDAAVYREMSDALANDTIYELFLHNITTAGHAGLVLPLRGAATAMLPALPRN
                     YFDLVFVDGDHSYAAVLADIQAAGGLIKDGGVLCGDDLERQSFEIDQAYARTQIESDY
                     IRDPRSGHEYHPGVTLAVGELFGEVSQVAGCWAVRKRGTGWERFDMSPARCSEDRIPA
                     HLIRRDPTVDQEFQRWREQRSRPASVAVPRGAERGEQVRTASAVSRASVAAPRQRALL
                     IQLDFQTWATARPWTYSAAFGVQEGLRANGVECVTVPAIAENACSTPASWVYHARKAL
                     AGQHFDQVWLWLIHTPLDQATLEWVAQLAPVRVGVLMESLRYDADDYSWAPQLKGRAA
                     HLEAQLPYLTHVLAPDEQDAAELRTRGFANVLWWPPMVPERFIVTPAGAPTQPQAVFH
                     GTPYGRRQHWVSEPSLSRRLQYAKGAQPPTNNQQLFDQLQQVAAQYLAEGHAVTDEAI
                     REYAQSLQQIRLAEFNEWMGHLAQWPAIVNLPSLAKFYGGRVIEAMAAGRPVISWDIP
                     GHPKNRGLFEPEKDILLFPQDAPEALAGQIDRILHDRALAESLAQRAQGKIKRYHTAE
                     RRLQGTLEWIRTGQTPDYGLSEQANSPSAAQTTVPAPAAQSSPSATGLPTADPISHPS
                     AKVSMDQNAFYVDLFVNKPAWSTPEPNADEAARWSKIASFLEHVVRQTRRETPGRTLR
                     ILDVGCGRGWLTNLATVYGTCEGIEPVAGVVEHARRMFPHIRFEAGTPETVLARPDFM
                     PFDIVLCSEVIEHVPHPEKPGFVSQLAQLLTSEGYLILTTPRGDVWEEWRRIAPPNQP
                     VEDWVTEQQLGQLLADGGFHHLGLERIPIEVPSLRYFPAATAHDYRTKTLMPIYQVWA
                     CRRTAATGTHPVPFTRRPMVSVIVPTHNRPDRLRMALESLNSQQYQDFEVIVVNDGTT
                     PVESVVAEMNCAGRTTLVNHDRNRGLAGARNTGLRHATGTYVAYLDDDDRFLSDHLGT
                     LVAFLERGTHQVAYTDAWRVTEQEAGAELRRDRPYSYEFDAQRLLVHNYIPVLCLMHR
                     RSCLDEVGLFDESLFVHEDWDLWIRLATVYPFAHIAQTTAEFTWRTDGSSMTSHDHDA
                     FCRTTDIIYRKYFPYVAAKPTMLEAQRHHLAGLKGRAKTPSYACSIVIPVWNNAALTR
                     QCLTALAEVTDDVTYEVILVDNGSTDGVQDFLRTLGGDVQVIRNEENLGFAKACNQGA
                     RAARGEFLVFLNNDTIPLKGWLSALVEDIRAHADVAVVGSKLLFEDGSIQHAGVAFSR
                     ECLMPYHMYRGGRAEAACANRRRELQCVTAACMLVRRRVFEQVDGFDEGYRNGFEDVD
                     LCLKIRKQAWKIVYQPKSVLYHLESKTPGRKIHELDNSQRLRERWG"
     assembly_gap    15436..15535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15536..16495
                     /locus_tag="PKK81_04275"
     CDS             <15536..16495
                     /locus_tag="PKK81_04275"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32116.1"
                     /translation="GRKIHELDNSQRLRERWGACWWVTDEDLLHFEDGYAIHTHVTDG
                     ILSYRLQVIADPETKTQRAILAEVQGAALRRDFNAVSAFLARVDEWPADPWILRWGVL
                     VCLGVAQPQLAIPFWRRILTFEEDAYARIGLAKQALETGAFDEADFHLTALLQQDPSH
                     GEGWLLRGIVAMQRNAYQQAEETFERAKLSGADSRKASLGIVMAAMGAGRAEAAWSQL
                     MTLCANEPDDEECMHWLLRCGTMLQRWEAVASRLAAFVARNPGNVAMRFALAGVLLRS
                     GRRGDSQREFERLRALSPTFEGMDELARQLAEAEGQLVPDHAA"
     gene            16485..17678
                     /locus_tag="PKK81_04280"
     CDS             16485..17678
                     /locus_tag="PKK81_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV32117.1"
                     /translation="MLHESTQNSRAMLIQLARLGDLVQSLPAIEALVDADPETPLDVL
                     CSAPLATVLGEARNIGRVIPWDGARWRAWGEQWSEDPHGTLRAAQAYLAGLGESTYGR
                     IYPLNQHNRSLLLTHLFSSPSVRADWDDRSEARIRPWAEYLRHIATHRGNNRVHLADA
                     WCGMSGVRPRGRAPLFQPPAVELPDDLAPIGERQGLWIALVTGAGETDRCVSPATWGR
                     WTKEFLTQTDAGQVVLIGSGRERETGQAILELIPTLLQGRVWDATGRTSIPQLMKLLH
                     KCRWVIGADTGPLHLGTLMGSRAIGFYFSHARVHETGPYGEGHWVYQHATQAPPDSWP
                     IRESIALICDDQRRAAADWTLWRNRMDHWGTSFDDGSEQERVEGARATVWRNLSPTLC
                     ESVAA"
     gene            17675..19492
                     /locus_tag="PKK81_04285"
     CDS             17675..19492
                     /locus_tag="PKK81_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3880 domain-containing protein"
                     /protein_id="HNV32118.1"
                     /translation="MNMFNDNVARLAVRDEPLAAAVKASAGGALTIEPARSGVPSARR
                     SGRWIHSAYDPLREADTWAETHAPACREGETVVVAGVGLLYHVEALRKRLASDIVVAV
                     LISDLKEFHDALVARPLGSWTDHILWLSGTPVEIADRLSKTGRALRCLSYAPATHTDS
                     DFHSAFEEALRRGVARQAGGQLTIALVGPIYGGSLPIARYVRRALETLGHKVHWIDHS
                     VHASSYEAMGTLKDARNRQLMQGRMAEVLSQWTLASLAESPPDLVLSLAQAPLTLPVL
                     EHLRKKKLLTAMWFVENYRHLTYWQQMAPGYDYWFVFQRGACLDAFRQAGARQVSFLP
                     MAADPELHRPMDLSDEERRTYGADVSFVGAGYPNRRRLFPALLRQPWSFKLWGNEWDG
                     ADELQSVLQLNGARIDTDTCMKVFNATAINLNVHSTTGAGLDPQADFVNPRTFELAAC
                     GAFQFVDSRSQLPEFFTDRELVSFRNFDDIPGLVGPWLGDPAARQAMAAAARTRVLGA
                     HTYVHRMRDLLGHIGLSQPDRVGAVLRGDRQQEALLSRCAGDAPLESLLKAFPAGQRV
                     ELKDVAARIRSKGSTATLKREELMVLMLDEYRSETRDLL"
     gene            19489..>20432
                     /locus_tag="PKK81_04290"
     CDS             19489..>20432
                     /locus_tag="PKK81_04290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV32119.1"
                     /translation="MSKQVLLINITRMGDLVQMGTLLQRLQHEWPGAAVDLVVDRRFA
                     PIAKLLPHLRHIIEYDFHRLVDESRAQTKDVVTLYREMTGWAAPLIEARYDRVVNLTF
                     NRRSGLLASYVGAKEIRGIAAPKDGDVAIHNPWMAYLTDVHSQRQFNHFNLVDIYALG
                     GSGTGPFAPLSLDIPPGTAQWARDFVAPYGGQRMPWTAVQVGASDPMKAWRPELFGQT
                     LAALSRQTPVGYVFIGTESERKAIETAQMAYRQAGGRGPLCDAVGRTTLPQLAAVLAQ
                     CRLLLTNDTGPMHLAVGVGTPVIDLSVGHVDFRETGPYG"
BASE COUNT         3989 a         5976 c         6218 g         4149 t
ORIGIN      
        1 tgcgggagat cgacctgttc cagctttgga tccaatgccc gaagaatggt gcgggtcggt
       61 tggtcgggcg gttggttttg aaggtcgact tcaaacggct cctgttggct gtgcccttca
      121 gcccccttga taattcgaat acgtggccgc aaacggtctt catgattcag accgacgacc
      181 agggcgcatt cattcgtatt gagcttgatg aggctcccga gagggtagac gcccagggcc
      241 ttgatggcga cttccaccag cgtcttctcg taacggcctt tcgctccaag cacgaagagc
      301 tgccgaattg catcgtgagg cagcaggggc ggacggccat gtcgcgcgct gactaaatcg
      361 tcataggtgt ccgccaaccc caccagttgc gccagcatgg agacagcttc ctgcttcagg
      421 tgttgaggaa acccgctccc gtcctgatat tcatgatgtt gcaggataat gcgggagacc
      481 gcatcgggga tcgagcccac ttgagtcagt acggtggccg ccaattgagg gtgttgcttc
      541 atgaggatct gctcctgatc ggtcagcgga gtggttttcc gatacaggtt gcgcgggagt
      601 cgcacatagc cgatgtcatg aagcagagcc cccagtccaa gagattccag gtcggcttcc
      661 gcacagccgt tttccacggc aagaatgagg gtgagcacgc acacatccat gccatgattg
      721 gcgagggtgg cgtcaaatcg tcggacttta tccagacaga actggatcag catcgcttcc
      781 ggctgtgcca cgatctgctc cagtagcctg ctcacgatcg gcttgagggc cgcgatcttc
      841 ggcgggttac ccgcttcgag gtcactgaag acgcgttcca cggcggccat gctttcccga
      901 taggccgcgg cagccactgc ggcctgtggg acttgcgcgc tttcctgggg cacggcggct
      961 tcaacgctta ccggtggcgg ttcatccgga ggaggcgcag tggccggcgc tggcggggcg
     1021 gcagccccga cgtccagccc gcggtcagta tcgatggtga cgacgaggat gccatggcgt
     1081 ttgagttgca cgatgtcgtc gggatttgaa accagccact tatggaggag aaacggagtg
     1141 cggtaccaag gctgatccaa accggcgata aacatgccga cggtgagttg atcgatgggg
     1201 atgtgctttg tcgaggccat atcagcgctg tacctactcc tcaggacgga ttgaactgag
     1261 cgcagtcctt ccggtgtttc atctatgctg tagagttcat atcggttgat ctgaagccgg
     1321 tctttacctg tgccgaccac gcagctccat aggctccttc aggtcttgca gccggcgacc
     1381 gatagagctg acgagggtgt gtctatgcgc ctgcaggcgc gggggaaaag catatgagcg
     1441 aactggtcat ctgtcgacat ccgtcctcgt ttctctcggt cggtctttcg cttcagctca
     1501 gcgtgccgaa ggacgaaacg caagggcagt atggctcatc ggtcctgggg tggaaacacc
     1561 ggagttggat cgtctgtgaa tggccgtttc atttgggaca gccgattccc tgcgtgaaag
     1621 ggaccctctg tctgttgcgg tatatctatg aaggaaaaat gatcggatat cgcgcggagg
     1681 tactggagac gcagatgcag cccttcccgt ttctgctgct cgcatttccc tccgagctgg
     1741 aggaggtgcc gctccggaag catggacggg tgtcggcaca ggagccgatc gtgctctctg
     1801 ccgtgcagga gtatgcatcc agctcgcgaa atgaaccccc gatccgcatc ggcgggctcc
     1861 tgaaggattt gagtgcctcc ggctgcgccg ttctcattca acgtccggtc caggactttt
     1921 acctggggat ggctctgcgt gttgagttcg aaatcatcgg caccggacac gtcaacaatc
     1981 tgacaggggt cgtccgaaac ctgtcactgc aggccgacgg aacgcacctc ggcctggagt
     2041 tccggttcga cggcaaagaa acgatcgagt atcggggatg gggaggatcg gtgcggaacg
     2101 cgcttgaatc cttcctgcat cggaaacact ccttcgagtc cgcctagact gcagcggttg
     2161 ccgttgactg ggaagttttt gcccggtcgt ttgcggcgac tcgttcccta cgcccagaaa
     2221 tcccgtcgtt tcaattcctc ccgccgcaca cgtcagtggc atacatcttg attggtatcc
     2281 cgttgccaca cctcgtcctg tgagggacgc aggccacgtt caggagcaag agggacgaca
     2341 atcatgacgc ttcacgcaca gagtgccaag ctcaatcctc gctggtgcct cgcgcaggat
     2401 acgatcgacc ggcaggatat cgaccatttg atcgagtggc tgcggaccta tcctcgcctc
     2461 accaaagggg cggtgaccct cgacttcgag cggcaatggt cggaatggtt agggcgtccc
     2521 tattcggtgc actgcaactc cggctcgtcg gccaatcttt tgatgtatta cgcattgctg
     2581 cgctcaggca agctgcgcaa cacaaccgtg atcgtcccga gtgtgggatg ggtgacgtcc
     2641 atcgccccgg caattcaatt cggctttaca cccatcatgt gcgaggccga tccggatacc
     2701 ttcggcctcg acctcaacca tctggaagac ctcttacaac gccatgacgc acagacggtg
     2761 ttactcgtcc aggtactcgg agtcccgcat cgcatgcagg agctgcagac cctgaaggac
     2821 cggtacgggt tttatctgct cgaagatgcc tgtgccgcca tcggcgcgga atatggcggt
     2881 aagaagatcg gcacattcgg cgacatggcg agcttctcct tctatttcgg ccaccagatg
     2941 tccacgattg aaggcggcat ggtgtcgacc agcgacaagc agttggcgga catgctctta
     3001 atgctgcgca gccatgggtg gagcaaggac ctcgaccaga gcagccatca ggcgttactg
     3061 acgcaatacc aggttgacga tttccactcg ccttttgtgt tttacgagcc gggcttcaat
     3121 ctccgctcga ccgatctcaa tgccttcatc ggcatcgagc aactgcacaa gctggactgg
     3181 atgaccggcc gacggcaggc caatcacgaa cggtacctgc agcacttggg tagccggttt
     3241 tacactcagc ggccgccgac agggagcaaa gttgccagca tctccttcgg gttgctcgca
     3301 gattcgacgg aacaacgccg tcggattgtc cgggcattgg ttacagaagg ggtggaaacg
     3361 agaattttct ccgccggcaa tctggggctt catcctttct ggatgaaccg ctacgggaag
     3421 gccagcttcc ctgtggcaga ccgcatacat cattgcggct tcttcctgcc caatcacgcc
     3481 tccatgaacg aacaggatgt ggctcacatc gcgcgcattg tcctggaggc cgcgtgacag
     3541 catcgttgta tatcagcagc tatgtattca caggggagct cgttcgatca tgacgacagc
     3601 ctcaacccat gtgctcgtga ccggcggcgc cggttatctc ggctccgtgc tcagcagagg
     3661 tctattggat cgggggtaca gggtcaccgt cctcgacaat ttcatgtatc gccagaacag
     3721 tttgatggac tgttgcgacg aggaggggtt tcaggtggtg cggggcgact gccgggacga
     3781 acgcctgctg acggatctgc tgcgaaccgt cgacatcatc gtcccacttg ccgccttagt
     3841 cggggcgccg ctctgcgacc gggaccgcgt cggggcctac acggtgaact tcgaagccgt
     3901 acaactcctg tgcaaactgt cttctccccg gcagcggatt attttccccg tcacgaacag
     3961 cggctacgga atcggtcaac cgggagtgcc ctgcacggag gagtcaccgc ttcgccccat
     4021 cagcctctat ggcgagacca aagtgaaagc cgagcgggtg gtgctggatc ggggaaacgc
     4081 catcacgctg cgcctggcga cggtgttcgg cgtgtcgccg agaatgcgaa tggatctgct
     4141 ggtcaacgac tttgtctggc gcgccgtgca ggatcgcgcc gtcgtcgtat tcgaagggca
     4201 ctgcaaacgc aactatatcc atatccggga cgtcgtccgg acgtttctgc atgccatcga
     4261 tcatttcgat gaaatgaaag atcgtccgta caacgtgggg ctggacgatg ccaacctgtc
     4321 gaaactcgaa ctctgccggg tgatccagcg attgatacct cacttcgtct cgttcgaggc
     4381 gccgatcggc gaggacccgg ataagcgcga ttacatcgtc tccaaccagc gtctcctggc
     4441 caccgggttc aagccggagt ggtccttgga acgcggcatt cgtgaattga tgaagtgtta
     4501 cacgatcatc aaagccggtc agtatgccaa tgtctgattc gagagcggat ggacggggag
     4561 ggcatcgatg attatcagtc gaacaccgtt ccgaatgtcc ttcttcggtg gcggcaccga
     4621 ttatcccgtg tggttccgtg aacatggcgg agccgtcctc gccacgacca tcgacaagta
     4681 ttgttacatc agctgccgcc gcctgccgcc gttcttcgaa caccggtcgc gcattgccta
     4741 ttcgcgtatc gagctggtga aaaacaatga agagatcgaa catcccgccg ttcgcggagt
     4801 cctgaagtat ctcaacatca acgacggcct ggagattcac cacgacgggg atctgcctgc
     4861 gcgaaccggc ttaggctcga gctcgtcgtt cacggtaggc ctgctccaca ccctttatgc
     4921 ccttcaacac atcatgccca gcaaagatca gctggcccaa gcggccatcc atgtcgagca
     4981 gaacgtgctt ggcgaagccg tcggttgcca ggatcaagtg ctcgcggcgc atggcagtct
     5041 ctgcaaagcg acgttctttc agaacggaga gatcgggcat accccgatca tcatgcaacc
     5101 cgaccggctt gccgcctttc agagccactt gcaactctac ttcacagggt tttcgcggat
     5161 tgcgtcggag gtggctcgcg agcagatcga tcgcacgagt caacgcaaag ccgaactgtt
     5221 tgccatgctg gaaatggtcg aagagggcat cacgatcctc accggcggcg gcgacctagc
     5281 tgcgtttgga accctgctgc acgaaggctg gatgctcaaa cgccgcttga cgtcacggat
     5341 caccactccg gccatcgacg agatttacac ggcggccagg gcggccggcg cactgggcgg
     5401 aaagttgctg ggcgccggag gcggagggtt catgctcctg tttgccaagc cggaggacca
     5461 tgagcggatt cgcagcgctc tgcccggatt gttgcaggtt ccgtttaagt ttgaaggcct
     5521 gggtacacag atcgtgtttt atcaggaaga tcatctcatg ctcgacgacg tgtgggctca
     5581 caactcggct gatacggcct cccaactgaa ggccgcgtta ctcggaaagg cagcgtgaag
     5641 atgggccggt ttgcggattg cgatgtggtc attctgtgcg gggggctcgg tacacgcctg
     5701 caatctgtcg tgacggatcg cccgaaatcg atgacggaga ttcatggccg cccgtttgtg
     5761 gcatgtctcg tcgaacattt tctgcgccat ggcacgcgtc ggtttatttt cagcaccgga
     5821 tacaagggcg acatgatcga ggactggttt ggccgccatc gaggcgggta tgaaaccctg
     5881 ttcgtccgcg accccgctcc gctgggaacg ggcggagcgg tcgcgcaagc gatgaagctc
     5941 gttcgcagca atccattttt ggtgctgaac ggagactccc tctgtgagct cgatccggag
     6001 cgactcctgc ggttccatac ccgtaaacgc gcccgcgcga cgatcgcggt cacccaagcc
     6061 gactcacggg aagataccgg agccgtcacg ttaggggagg acgaccgcgt cctgtccatg
     6121 gtggagaagc cgcgcaccag aacgacgggc tatcacaatg ccggcatcta tctgttcgat
     6181 cgtaccgtcg aggcgctgtt tccgaacagt cgttcctggt cactggagcg cgagttactt
     6241 ccgcagctgg tcaccgaacc gttctatgga ttcgtcacag ccagcgcgct gtacgacatc
     6301 ggcacccccg agcgcctcgc gcattttcgc gaggtctgga aagacccgtc aattcatttt
     6361 ccggtcacat ccatgcatca ggaacaagga tcgttgttgt aatggctcat cggacagtcc
     6421 cgggttcaca gacaacgcgc cgattggcat tttcatcgcg aggtgaacgc ctgatcgggc
     6481 aggagatgtt ccgggtcatg gatcgggctc aagccctgga gcggcagggg tatcgcatct
     6541 atcatctgga actcggcaat ccccgcatgg ctccgccggg tgaaatcgtc gacggcaccg
     6601 tgcaggcgct ccgcgagaaa cagctgggat atgcgccgat ggccggcgtg aaggaattac
     6661 gagccgcgtt ggcggctcga tacacctcgt tgacgggcga ggcgctgacc gactcccagg
     6721 tcgtgatcag ccctgcgaat ctcctgattc atcagtttct ggacatcact tgcaatcccg
     6781 gcgatcgcgt ggtgctgttc acgccggcat ttccctccta ctgggccgct gcgacgcatc
     6841 agggacttca ggtcaggccg gtgccgcttt ctgagcgcga cggatttcac ctgacccgac
     6901 aggccatcga tgacgcgctg gctgccggac caagggcgat tgtcgtcaac aacgccaaca
     6961 accccacggg ggccctctac gattcgacga ttctgcgcgc gcttgcggca cgctgtgaag
     7021 aggcggggat ctggctgtta agcgatgaaa cgtacgccga tctgaccttc gatggttcgt
     7081 tcgtgaccct cgcctcggcg cgagccggat acgtcgtagc gatgtcgtcc ttttcaaagg
     7141 tgtgctcgat cccagggttt cgaaccggat atgccttcgc ccatgaggcg gtggcggcaa
     7201 aactcgcgct ctccaattcc accctctatt cctgtcttcc cctgtttact caactgggtt
     7261 gcttggccgg tatgaaggta ctcgatacct atgcgaacga ggttcgatca cgcggctccc
     7321 gcctcatcgg atcctgccac gaccgcatca accgctccgg catcctgcat tgccacaagc
     7381 cggagtcagg gttttatctg tttgtcgata tcgcgcgcac cgggctcgac gatatgacct
     7441 tttgccggcg actactggac gatcaccata cggcggtcac cccaggccgt agtttcggcg
     7501 aagcctacgc gtcgttcatt cgcatagccg tgtgcgggca ggaagaggat gtgcacgaag
     7561 gcctgtcgcg tacgatcgcc tttgcgcaac aactgggagg ttgccgtgtc caagccgctt
     7621 gacgtgttgc tgatcacccc gcctagtcgg gtacaggtgt atcaggagtt gagccgggac
     7681 ttcgccgcca tcgaaccgcc ggtctggtct ggtttgatcg cgaccttcct gcgacaacac
     7741 agctgctccg tggccattct ggatgccgag gcagaggggt tgaaccacca gcagacggcg
     7801 gatcggatcg ccgccattgc gccgagactc gcggtcttcg tgatctatgg gcaacaaccg
     7861 tccgcctcca cacaatgtat gccggccggc cggaaggtgt gcgaaatcct gactgcgctg
     7921 gccgatattc ccacgctcgt gatggggact cacccgtcgg ccctgccgaa gcgcaccctg
     7981 ctggaagagc cctataccta tgtctgccag ggagaaggtc ctgccacgat tctcggtctg
     8041 gtgattgcct tacgcgcacc gcaacattcg cttcgcgagg ttcccggctt atggcacatg
     8101 gagaagggtg tgccggtcgg caatgcgccg gcgcaactgc tgacgaatct ggattgcgag
     8161 gtgccggggc aggcctggga tctcctcgac atgacccgct atcgcgccca caactggcat
     8221 tgcttcggca atcttgaggc gcgggctccg tacgcgtcgt tgcagacgag tctgggctgt
     8281 cccttcacct gttcgttctg ttgtatcaat tcacccttcg ggactcccat gttacggacc
     8341 tggagcccgg acaatgtcat tgcgcagatc gaccggctgg tccgggacta tggcgtcacc
     8401 aatatcaaaa tccccgatga aatgtttgtg ctgaatcggc ggcatgtgat cggcatttgc
     8461 gatcgcatca tcgaacgagg ataccggctc aatatctggg cctacgcccg ggtggacacg
     8521 gtccaggacg aagtgttggc gaaactcgcg cgggcgggat ttacctggct ggggctcggc
     8581 atcgaatccg gaagccagca tgtccgcgac ggcgtcgaaa agggccgctt cgtcgaacgg
     8641 gacattgtgg cgacggtcga caagatccgc tcgtacggca ttcatgtagc ggccaactat
     8701 atcttcggcc taccggacga cacgatggag agtatgcgcg cgaccttgga cttggccctc
     8761 acgctgaata cggaatgggc caacttttac tgtgccatgg cctaccccgg ctcgccgctg
     8821 tatgacctgg cgaagcagaa gcactggacg ctgccggatg atgaaggcgg ccccggatgg
     8881 atcggttatt cgcaacatgc ttacgagtgc tctcccttgc cgaccgacag cctcacggcc
     8941 acacaggtgt tggcgttccg ggatcgcgca ttcatggagt atttcaccca tccggactat
     9001 ctcggcatgc tccggcagac attcggagcg caggtcgcca cccatgtaga gggcatgtgc
     9061 cggcaccagg tccgccgtcg tcatcacgat gtggtcaccg atgcagttgc gtagcgacag
     9121 agaggggaaa ggagcggtca tgatcaaggt tgcagacttc atcattcata cattggcgga
     9181 gcgcggaatc gacaagatgt tcgtggtcta cggcgccgcc aacggtgacc tgatcgatgc
     9241 gtttacacgc accgccgcca cggaatacgt cgccgtcatg cacgagcagg ccggcgggtt
     9301 tgcagccgag gcctatgcaa aagtgaaggg cgtgcccggt gtagccatcg ccaccagcgg
     9361 accgggcggg atgaatcttc tcacggcgat gggcaactgc ttttatgact ccgtgccctg
     9421 cgtctttatg accggccaga tcaattcccg gttcctccgg cccgatcctg ccattcgcca
     9481 gatcggattc caggagaccg acatcgtcgc catggcggct ccggtcacga agtacgcgaa
     9541 aatgattctg aagcccgaag atgttcggta tgagctggag aaagcgctgt ggctttgcca
     9601 ggaaggacgg ccgggaccgg tactgttgga tattcccctc gatgtccaaa agacgatgat
     9661 cgatgccaag aagctcatcg gcttcgagcc cccggccgcc gtgagtttcg acctcacggt
     9721 ggtcgatgag cagatctccc ggtttatcac cgagctccaa catgctgaac gaccggtcat
     9781 tctcgtcggc ggcggcgtcc gcctcgcgga tgcccaggat gacgtgcgcg ccttgggacg
     9841 gcggttgaac gtgccctgtt ttccgacctg gaatgccttg gacgtgatca gctcggacta
     9901 tgaaaactac ggcggccggg tcggcacgta cggaggggcc ggccgcaact tcggcattca
     9961 gaacagcgat gtgctcctct cgatcggcag ccgcatctcc ggccgcatca ccggcggaaa
    10021 tgtccagagc tttgcgcgcc aggccaagaa gttcctggtg gaaatcgatc cggccatggt
    10081 gcaacggaag tttcagcagg ttcctttcga tgtgaacatt ctctgcgacg ccaaggtgtt
    10141 cactcagcgc ctgttgatcg ccctcgatcg gcggagcaaa ccattgccga actattcggg
    10201 ctggacagaa cgggtgatgg aatggaaacg ccagtacgat cccgtccggc ctgaattttt
    10261 cgcccagcgt gagcgggtgc acccctatgc attcatgcgt cgcctttccg aaaagatggg
    10321 tgccacggat attctcgtcg gggattgcgg cggcaatatc gtcgtcagca accatgcctt
    10381 tgaaactaaa tacgggcagc ggaatctcac gaacaacggc aactctccca tgggattctc
    10441 gtttgccgga gcgatgggtg cctggtttgc cgcgccaagc cgtcaagtgg tctgcaccat
    10501 cggggacggg ggcttcaaca tgaatcctca ggaactgcag accttcatca attacggcgt
    10561 caaagttaaa accttcattc tcaataatca catttacgga atcaccaagg cctaccagga
    10621 gaccaatttt cagggccgcg cggaggcctg cgggccgaag ggctacaacc ctcccgattt
    10681 tctgaagatc gtgcaggcct acggaatcaa gacggtggcc atccacaacc atgccgagat
    10741 ggatgcgaag attgaagagg tactgcaatt cgacgggccg gtggtctgcg atgtggacat
    10801 gcatgaattc cacacctacg agcccaggat cttcggatgg aagacgccca tcgaggatat
    10861 gtatccctat ctgccgcgtg aagaattccg agccaacatg gttatcgaac cggctgaggg
    10921 ctggatgaac ccggaatacc cggacgtcgt tcgccggaac gatcggtcac agccgtaagg
    10981 ggactgatat gtcgcaaggt gcacgtgaga caaaccgagt gactccgctg gacgttgagt
    11041 actaccaatt ctgccgcgaa cagctgcgtc cctatttcgg tcgggtgatg tgggcatcgc
    11101 aaggactccc gcttcgccac gtagtgatgc aggaactggt gcggctggag gcctcccggc
    11161 aaggtgcagg tccgtttcat attcttgagg tgggatcatg ggccggggga tcggctatta
    11221 cgtgggccga ggcgttgacc cggcatcatc gagccgacgg ccgtgtcgtc tgtgtggatc
    11281 cctggaagcc gtatttcgat gtgaagaagc ggccggacgc ggccgtgtac cgtgagatgt
    11341 ccgacgcctt ggccaacgat acgatctacg agttgtttct gcataacatc accactgccg
    11401 gccatgcggg gctggtcctt ccgcttagag gggccgccac agccatgttg ccggcccttc
    11461 cgcggaacta tttcgatctc gtctttgtcg acggcgacca ctcctatgcg gcagtgctgg
    11521 cggatattca ggcggccggg ggcctgatca aggatggggg cgtcttgtgc ggtgacgatc
    11581 tggaacgtca atcattcgaa atcgaccagg cctatgcccg cactcagatc gagtcggact
    11641 acattcgcga tccgcgatcg gggcacgaat atcatcccgg cgtcacgttg gccgtggggg
    11701 aactcttcgg tgaagtgtcc caagtggcag ggtgctgggc ggtgagaaaa cgcggcactg
    11761 gttgggaacg cttcgacatg tcaccggcgc gctgttctga ggatcgtatt cccgctcatc
    11821 tcattcgccg ggaccccacg gtggatcagg agtttcaacg ctggcgggaa caacgaagcc
    11881 gaccggcgag tgtggctgtg cccaggggag cggaacgcgg cgaacaggtc cggactgctt
    11941 cggcagtttc cagagcttct gtcgccgctc ctcggcaacg agccctgttg attcaattgg
    12001 atttccaaac ctgggccacc gcacgccctt ggacctatag cgcagctttc ggtgtgcagg
    12061 aaggtttgcg ggcgaacggc gtcgagtgtg tcaccgtgcc ggcaatcgcc gagaacgcct
    12121 gctccacgcc tgcgtcatgg gtctaccatg ccaggaaggc cctggccggt cagcacttcg
    12181 atcaggtatg gctctggctc atccatacgc ctctcgacca ggctacgctg gaatgggtcg
    12241 ctcaattggc gccggtgcga gtcggggtcc tcatggagtc gcttcgctac gatgcggacg
    12301 actattcctg ggcgccacag ttgaaaggtc gtgccgcaca cttggaagcg caacttccct
    12361 atttgaccca tgtgctggcg ccggacgagc aggatgccgc cgagctccgg acgcgtggat
    12421 tcgccaacgt gctctggtgg ccgccgatgg ttccggaacg gttcatcgtt actcccgccg
    12481 gcgctccgac ccagccacag gctgtcttcc atggaacgcc gtatgggcgg cggcagcatt
    12541 gggtgagtga gccatcactg agcaggcgtc tgcaatacgc gaaaggggct cagcctccta
    12601 cgaacaatca gcagttgttc gatcagttgc agcaagtcgc ggcccaatac ctggcagaag
    12661 gccatgcagt caccgacgag gctatccggg aatatgccca atccttgcaa cagatccgcc
    12721 tcgccgaatt caacgagtgg atggggcacc tggctcaatg gccggctatc gtgaacctgc
    12781 cgagcctcgc caaattttat ggcggacggg tcatcgaagc catggcggcc ggtcgaccgg
    12841 tgatctcctg ggacattccc gggcatccga agaacagggg actcttcgaa ccggagaaag
    12901 acatcctgct gttcccccag gatgcgcctg aagcccttgc cgggcagatc gaccggattc
    12961 tgcatgatcg cgcattggcc gagtccctgg ctcagcgggc gcagggcaag atcaaacggt
    13021 atcacacagc cgaacgacgc cttcagggaa ccttggagtg gattcgcacc ggacagactc
    13081 cggactatgg cttgtcggaa caggcaaact ccccatccgc agcccagacc acggttccgg
    13141 cgcctgcagc tcaatcctcg ccgtccgcca cagggttgcc gacggcagac ccgatttccc
    13201 acccatcagc caaggtgtct atggatcaga atgcctttta tgtagaccta ttcgtgaaca
    13261 aaccggcttg gtcgacaccc gaaccgaacg ccgatgaagc ggcgcgctgg tcgaagattg
    13321 cgtcgttcct tgaacatgtc gtgcgacaga cgcggcgcga gactcccggt cgcacgctac
    13381 ggattctcga tgtgggttgt ggccggggat ggctgacgaa tctggcgaca gtctatggga
    13441 cctgtgaagg catcgagccg gtggccggcg tggtcgagca tgcgcgtcgg atgtttcccc
    13501 acattcggtt cgaggcgggc acacccgaga cggtgttggc caggccggac ttcatgccgt
    13561 tcgatatcgt gctctgttca gaagtcattg aacatgtccc ccatccagag aagccggggt
    13621 tcgtcagcca actggcgcag ctcctcacca gtgaaggcta tctcattctg acgactccgc
    13681 gcggggatgt gtgggaagaa tggcgacgca tcgctccgcc caatcagccc gttgaggatt
    13741 gggtgacgga gcagcagctg ggccaactat tggccgacgg tggctttcat catttggggc
    13801 tggaacgcat tccgatcgaa gtgccttccc tccggtattt cccggcggcc accgcccatg
    13861 actatcgaac caagacactc atgcccattt atcaggtctg ggcctgccgt cgcacggcgg
    13921 cgacggggac gcacccggtg ccgtttacac gacgaccgat ggtgtcggtg attgtgccga
    13981 cccataatcg accggaccgt ttgcgcatgg ccttggagag tctcaactct cagcagtacc
    14041 aggatttcga agtgatcgtg gtcaatgacg gcacgactcc ggtcgaatcc gttgtcgctg
    14101 agatgaactg tgccggacga acgacgcttg tgaatcatga ccgcaaccgg ggactggcag
    14161 gggcgcgcaa caccggcctt cgccatgcca ccgggaccta cgtcgcctat ctcgatgacg
    14221 atgaccgctt cctgtccgat cacctgggca cgctcgtcgc ctttctcgaa cgtggcaccc
    14281 atcaggtggc ctacaccgat gcctggcgtg tcaccgaaca agaagcggga gccgaattgc
    14341 gtcgtgaccg gccctactcc tatgagttcg atgctcagcg tctgcttgtt cacaactata
    14401 ttccggtgct ctgtctgatg caccggcggt cctgcctcga tgaggtgggc ttgttcgacg
    14461 aaagtctgtt cgtccatgaa gactgggatc tctggatcag gctcgccacc gtctatcctt
    14521 ttgcgcacat cgcgcagacg accgcagagt tcacgtggag aaccgacggc tcatcgatga
    14581 ccagtcacga tcacgatgcc ttctgccgca ccaccgatat catttatcga aaatactttc
    14641 cgtacgtggc ggcgaagccg acaatgctgg aggcacaacg acaccatttg gcggggctga
    14701 agggccgggc aaaaacgcca tcgtatgctt gctcgatcgt tattcccgtc tggaacaatg
    14761 cggccttgac ccggcaatgt ctgacggcgc tggcggaggt caccgacgac gtgacctatg
    14821 aagtgattct ggtcgataac ggatcgacgg acggcgtgca ggattttctc cggacgctcg
    14881 ggggtgacgt acaagtgatc cgtaacgaag agaacctcgg atttgccaag gcctgcaatc
    14941 agggcgcgcg ggcggcacgc ggcgaattcc tggtgtttct gaataacgac acgattccat
    15001 tgaagggatg gctgtcggcg ctggtcgagg acatccgcgc ccacgcggat gtggcagtgg
    15061 tgggcagcaa actcctcttc gaagacgggt cgatccaaca tgccggcgtt gccttctcac
    15121 gtgaatgtct gatgccttac catatgtatc gcggagggcg cgccgaggcg gcctgtgcaa
    15181 acagacggcg cgaattgcag tgcgtcaccg ccgcctgcat gctcgtgcgt cgtcgtgttt
    15241 tcgagcaggt ggatggattc gacgaaggct atcgaaacgg gtttgaggat gtcgatctct
    15301 gtctcaaaat tcggaagcaa gcgtggaaga tcgtctatca acccaagagt gtgctctatc
    15361 acctggaaag caagacaccg ggcaggaaaa tacatgaact cgacaacagc caacgcctcc
    15421 gggagcgctg gggcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcag
    15541 gaaaatacat gaactcgaca acagccaacg cctccgggag cgctggggcg cctgttggtg
    15601 ggtgacggat gaagatctcc tgcactttga agatggatat gccattcaca cgcatgtcac
    15661 cgacggcata ttgagctacc gtcttcaagt gattgccgac ccggagacca agacccaacg
    15721 ggcgatcctg gcggaggtcc agggggcagc tcttcgccgc gacttcaatg ccgtctccgc
    15781 cttcctcgcg cgcgtggatg agtggcccgc agacccatgg atcctgcgtt ggggggtgct
    15841 cgtctgtctc ggggtcgccc aaccccaact ggctattcca ttctggcgac gcatcctcac
    15901 cttcgaggaa gacgcctatg cccgtatcgg actggcgaaa caagcgctgg aaacaggtgc
    15961 gttcgatgag gcggacttcc atttgacggc gctgctgcaa caggaccctt cgcatggaga
    16021 aggttggcta ctgcgcggga tcgtggccat gcaacgcaac gcgtatcagc aggcggaaga
    16081 gacgttcgag cgggcgaaac tctccggtgc cgattcacgc aaagcctcct tgggcatcgt
    16141 catggcggcg atgggtgccg ggcgggccga agcagcctgg tcgcaattga tgaccctttg
    16201 tgcgaatgag ccggacgacg aagaatgtat gcattggctg ctccgatgcg gaaccatgct
    16261 ccaacggtgg gaggcggtcg cctcgcgact cgcggcattt gtcgcccgca atccgggcaa
    16321 tgtcgccatg cggtttgcct tggcaggggt gctcttgcga tccggacggc gaggggactc
    16381 gcaacgagaa ttcgaacggc tgcgcgcact gtccccgaca ttcgaaggaa tggacgaact
    16441 ggccaggcaa ctggccgagg ccgagggcca actggttcca gaccatgctg catgagtcca
    16501 cacaaaacag ccgggcgatg ctgatccaac tggccagatt aggagatctg gtgcagtccc
    16561 ttcctgcgat cgaagccttg gtggatgccg atcctgaaac gccattggat gtcctttgtt
    16621 cggccccctt ggcaacggtc cttggtgaag ctcgaaacat cggccgtgtc attccctggg
    16681 atggcgcgcg atggcgggcc tggggcgagc agtggtcgga agacccgcac ggaactcttc
    16741 gcgccgcgca ggcgtatctt gcagggctcg gggagagcac ctacggccgg atctacccgc
    16801 tgaatcaaca caaccgtagc cttctgctga cgcatctatt ttccagcccg agtgtgcgcg
    16861 cggattggga cgaccgttca gaggcgcgca tacgtccatg ggccgagtat ctccggcata
    16921 tcgcgacaca ccgtgggaac aatcgggtgc atctggccga tgcctggtgc ggcatgagtg
    16981 gagtcaggcc acgaggtcgg gcgccgctct tccagcctcc ggccgtcgag cttcccgacg
    17041 acctcgctcc catcggcgaa cggcagggtc tctggatcgc gttggtcacc ggcgcaggag
    17101 agacggatcg ttgtgtctca ccggccacat ggggccgatg gaccaaagag tttctgacac
    17161 agacagacgc aggacaggtc gtgctgatcg gaagcggacg cgagcgtgaa accggacagg
    17221 ccattctcga attgattccc acgttgctcc aagggcgtgt ctgggacgcg accggccgca
    17281 caagcatccc tcagttgatg aagctcctcc acaagtgccg atgggtaatc ggagccgaca
    17341 ccggtccgct tcatttgggg acgttgatgg gaagtcgagc cataggattc tatttttctc
    17401 atgcccgggt gcacgaaacc ggcccatacg gagaagggca ttgggtgtat caacatgcca
    17461 cgcaggcccc gcctgacagc tggcccatca gggaaagtat cgcgttgatc tgcgacgacc
    17521 agcgtcgtgc ggcggccgat tggaccctct ggaggaatcg tatggaccac tgggggacga
    17581 gttttgatga cggttcagag caggagcgcg tcgagggcgc ccgcgcgact gtctggcgaa
    17641 acctctctcc gacactgtgc gaatcggttg ccgcatgaat atgttcaacg acaacgttgc
    17701 ccggttggcc gtaagggatg aacctctcgc tgcggcggtg aaggcctcgg cgggcggcgc
    17761 actcacgatc gagccggcgc ggagcggcgt gccttcggcg cgacgatccg gccggtggat
    17821 tcacagcgcc tatgatccgc tccgcgaagc ggacacctgg gccgaaaccc atgcgcccgc
    17881 ctgtcgggaa ggtgaaaccg tcgtcgtggc cggcgtcggg ctgctgtacc acgttgaggc
    17941 gcttcggaaa agactggcat cggacattgt ggtcgcggtg ctgatctccg atctcaagga
    18001 attccatgat gcgctcgtcg cacggcccct cggatcatgg accgaccaca ttctctggct
    18061 ctccggaacg ccggtcgaga tcgctgaccg gctgagcaag acgggccgcg ccctgcgctg
    18121 tctgtcctat gctccggcca cacacacgga ttcagacttc cacagtgcgt tcgaggaagc
    18181 cttacgtcgc ggagtcgcgc gacaggcggg tggacagctg acgattgcgc tggtggggcc
    18241 catttacggc ggttcgctgc cgattgcgcg ttatgtgaga cgggctctgg aaaccctcgg
    18301 acacaaggtg cactggatcg atcacagcgt gcatgcgtca agttatgagg cgatggggac
    18361 actgaaagat gcgcgcaatc gccaactgat gcaaggtcgc atggccgaag tcttgagcca
    18421 atggaccttg gcctctctgg ccgagtcacc tcccgacctc gtcttgtcgc tggctcaggc
    18481 cccgttgacg ttgccggtgc tcgaacatct ccgcaagaag aagttgctga ccgccatgtg
    18541 gttcgtggag aactatcgcc acttgaccta ttggcaacag atggcgccgg ggtatgacta
    18601 ctggttcgtg tttcaacggg gggcctgtct cgatgccttc cggcaggcgg gtgctcggca
    18661 ggtcagcttt ctgcccatgg cggccgaccc cgagctgcat cgcccgatgg atctgtcgga
    18721 tgaggaacgg cgcacctatg gtgcggatgt ctccttcgtc ggcgcagggt atccgaatcg
    18781 ccggcgtctg ttcccggcac tgttgcgcca accctggtcg ttcaaactgt gggggaatga
    18841 gtgggacgga gccgacgagc tccaatccgt cctccaactc aacggcgcac ggatcgatac
    18901 ggacacctgc atgaaggtgt tcaacgccac ggcgatcaat ttgaatgtcc attccacgac
    18961 cggcgcgggc cttgatccgc aagccgattt cgtcaatcca cggacctttg aactggcagc
    19021 ctgcggcgcc tttcagttcg tcgattctcg ctcccagttg ccggaatttt tcaccgaccg
    19081 ggagttagtc tcgttccgga acttcgatga cattcccgga ctcgtggggc catggctcgg
    19141 agatccggcg gctcggcaag ccatggccgc tgccgcccgc acacgtgtct tgggcgcgca
    19201 tacctatgtg catcggatgc gggacttgtt gggacacatc ggattgtctc agcccgatcg
    19261 agtcggggcc gtgctgcgag gagaccgaca gcaagaagcc ctgctctcgc gttgcgccgg
    19321 agatgcgccg ctggaatcgc tgctcaaggc ctttcctgcc ggacagcggg ttgagttgaa
    19381 ggatgtcgcc gcacgaattc gcagcaaagg ctcgaccgcg acactcaagc gggaagaatt
    19441 gatggtgttg atgttggatg aataccggag cgagacacgc gatctgttat gagcaagcaa
    19501 gtgcttctca tcaatatcac ccggatgggc gatctcgtgc aaatgggcac gttgctacaa
    19561 cgcctccagc atgagtggcc gggagccgcc gtcgatctgg tcgtggatcg acgcttcgct
    19621 ccgatcgcga aactcctgcc tcatctccga cacatcatcg agtatgactt tcatcgcctg
    19681 gtcgacgaga gtcgcgcgca aacgaaggat gtcgtgaccc tctaccgtga gatgacaggc
    19741 tgggcggcgc cgttaatcga ggcccggtac gaccgcgtcg taaatttgac cttcaatcgc
    19801 cggagcggac tgctggcctc ctacgtcggc gccaaagaga ttcgaggcat cgcggctccg
    19861 aaagacgggg acgtggccat ccataacccg tggatggcct atttgaccga tgtgcacagt
    19921 caacggcagt tcaatcactt caatctggta gatatctacg ccttgggcgg aagcggtacc
    19981 ggaccctttg cacccttgtc gctcgacatt cctccgggca cggcgcaatg ggcacgtgat
    20041 tttgttgcac cgtacggcgg acaacggatg ccctggacgg cggtgcaagt cggggccagc
    20101 gatcctatga aggcctggcg accggaactc ttcgggcaga ccctggctgc gctgagtcgg
    20161 cagacacccg tcggatatgt ctttatcggg acggagagcg agcggaaggc gattgaaacc
    20221 gcacagatgg cctatcggca agccggcgga cgcgggcctc tctgcgacgc ggtcggacgg
    20281 accacgctgc ctcagctcgc tgcggtgctg gcccagtgcc gactcctgtt gaccaacgac
    20341 accggcccca tgcacctcgc agtaggagtg ggcactccgg tgatcgatct ctcagtcggg
    20401 catgtcgact ttcgcgagac gggtccctat gg
//
LOCUS       DAOQRM010000029        20099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1842_length_20099_cov_13.720166, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000029 DAOQRM010000000
VERSION     DAOQRM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20099
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1842_length_20099_cov_13.720166"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(679..765)
                     /locus_tag="PKK81_04295"
     tRNA            complement(679..765)
                     /locus_tag="PKK81_04295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(729..731),aa:Leu,seq:taa)
     gene            complement(921..1172)
                     /locus_tag="PKK81_04300"
     CDS             complement(921..1172)
                     /locus_tag="PKK81_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNV32120.1"
                     /translation="MSTKGREAAKIAAIIGGGAVVGAALGLLLAPKTGAETRRDVARY
                     AKRAQVQATRFSRSVKSGVSTMVERSKALVKRDDHKEAA"
     gene            1499..2332
                     /gene="lgt"
                     /locus_tag="PKK81_04305"
     CDS             1499..2332
                     /gene="lgt"
                     /locus_tag="PKK81_04305"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNV32121.1"
                     /translation="MLSWLHAIPYPDIDPVFLRVGPLQFRWYGLMYLIGLTLAYFIIA
                     ARARAQKLPMNNDQVYDMIVYAAVGVFAGGRLGYVLFYNLSYYLENPLKIFAVWEGGM
                     SFHGGLIGTIVALILFARRQGMTVLTIADLAAGVTPVGLGLGRIGNFINGELYGRPTD
                     VDWCMVFPAGGMACRHPSQLYEAFLEGLLLFTVLWLITRRLPPSGTVFGAFLMGYGIC
                     RIVVEFFREPDAQIGFLVGTISMGQMLSIPMIVAGAVILAIAAQRKRPLQPDSGTAAP
                     L"
     gene            complement(2358..2762)
                     /locus_tag="PKK81_04310"
     CDS             complement(2358..2762)
                     /locus_tag="PKK81_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32122.1"
                     /translation="MRAFRWAVLVMMAVVGCASQHKAEVRPLHAAAGTSAVVSQQLEQ
                     GNQLFAQQDWAGAQQVYLATIQADQTLAEAHYNLALTLERLGEKAEARKHYVAAANLA
                     PGNKVIWDAPPLRKYDRELGLDRKSWMDANPK"
     gene            complement(2780..6166)
                     /locus_tag="PKK81_04315"
     CDS             complement(2780..6166)
                     /locus_tag="PKK81_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNV32123.1"
                     /translation="MTEPAQIPDQAAREAAATTFDRNVVVIAGAGTGKTTLLVNRLLY
                     LLMRRTDPLDLSRIVALTFTNKAATEMKLRLRERLRSLVNVEGRDNPAAGSGTVSIAD
                     LRMRYGWTTDEIVARADAALRDVEKAQIGTLHSFAAHLLRLYPIEAGVTPTFQTDEDG
                     SRFEEHFTSEWELWLDGELGAAGSDHGRWRILLDTFSLDELRELAYSLHSDLIDLNGL
                     MQQVAGTGLNKGLRDWFSLRKAQAERLLARYDRPKRRKIELMLAAVAELFALLLAEGL
                     GALRSFPREPQELLAKDLGAAPTGWEEDDYEAVESLQRIAKRLLRVDHELLQNLLALL
                     APFVQSVRKSFLDSGWLTFDGLVGKARTLLRDHPPIREQLKRDYRALLVDEFQDTDPV
                     QYEIVLYLAERPGTQAGSWRETELESGKLFIVGDPKQSIYAFRRADIEAFDHVVDRLE
                     GSGALRCELATNFRSHAQVLDLVNGVFNRLLVAQPAIQPPNVPLTVQPNRSSQFRNPG
                     VELRLVVSEDDGDLDSAAATRVEAEQIALMVSGLLRPAGQGGEAGGPESGLRPGHIAL
                     LFRKLTQAEHYLEALRRHGIAYIIDGEKHFYRRQEVIDLVNILRCVDNPHDRIALVGL
                     LRSAVGGLPDSALVGLQERQALDYREVERLTDWSSPHAEPLRRLYGRLVRLHEQAPRC
                     PLPEVLDLIFAQLPVLELAAASLHGEQAVANLFKVRQMAADLADRPNLTLNGFVSLML
                     DRLTEQPEEAESALSEDTLDAVRVLTIHKAKGLEFPLVILAGLHHGDGAGRGPARPLI
                     WHDWSTGVQGLDLGDRCSLGAVLVAEKARTREQAERRRLFYVGMTRARECLVLSGALP
                     RRRVRGALLELLEEAAGAELGLAEQQDIPVGAVGLRQIVLQGDDRPPARQRERPVTLE
                     GAVMDRAFSERWMQRDRNWQAQRSESLLVSPSNFIQKPAPASEREPGRVRRVTPGKTV
                     GTIVHRLLQYWDFTADVASQVALIDHTSLALDEPDTAMQEAVIEEVRDLLRTFAQSAA
                     YDRLRRATVIGREVPFLVPWNGGRQIMEGVIDLLYRIDGALWIADYKTDMIPADQVAA
                     RAELYREQARLYRAAVEQSSGEPVAGFEFIFLRHGVAVTV"
     gene            complement(6159..>7002)
                     /locus_tag="PKK81_04320"
     CDS             complement(6159..>7002)
                     /locus_tag="PKK81_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNV32124.1"
                     /translation="LTPPAMGQLCHDALRFCYLALIQQGWPATTLPASVIIAEVNQAV
                     AQAFEAYALTHGTGYALTWELAQESVRRLIEATVTLDRKMALVSGFHPAEFELVANGL
                     LPDGSGGEDIPLRGRWDRVDRHPGSGAVRVIDYKYRANDRVEAKDRNLLQAALRAQRL
                     QPALYALMTPAGSSGDREKALPEQVEFLYLLPHADPAVERVSFAASAWQGPSGPMLSR
                     TMQVLLDGIRAAQYFIVPDAHCTHCEFSTACRRAHQPSWWRAYRSSQARTLRDVRSQK
                     VARD"
     assembly_gap    7003..7102
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7103..9544)
                     /locus_tag="PKK81_04325"
     CDS             complement(<7103..9544)
                     /locus_tag="PKK81_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNV32125.1"
                     /translation="MLRLVTGPFHPTLESQLVHDLRVLKSGDPQAAVALVVPSDQLRR
                     SLKRLLVVKRGLALLNVHILSFHQLALQLLRERRTTAGAAGAVRQLELVTDTFFERLL
                     QHLGQRNVPQTGALRLPQLPSGAWPALWASVRDLKDATVDSALALRAVEEGQFPPEDG
                     EKLKGLFTLYAALRDGSAALGVGSPDDLAALVTDFVPASPFLRSLNGLWYYGSYDLTQ
                     TQLTLLESLSISLPVTVYFPLSERPAYGFARQFLERHLYPIAGGVDERAGAPSGDGAH
                     ASQEVNVSVEVRNAAGIDDELTLVCKQILSLVETNGFAFDEIGVVGRTLVPYQASLKR
                     TFDQHRIPFLSSATVPLLQEPSVKTLLHLARLKGSGLHRPAMLEVITSSWNRRLTANR
                     GGVEPRPDLWRLAVLALGITRGEEEWRRLAQLGRLESWSSDADDPVTEELESLSIDGV
                     QLRLLWGCLSPLIDDVKRLPEQGGYGTLTDAFCSLAETHLTVPLGVSTSIDATAQWDD
                     ATDVNEALSRVFAQLRALDRLDINLTWDEWTETFAEILARTSRALAPSSHHGVQVLDA
                     MAARGVGFRALFLVGMNEKLFPRYIHEDGFLRDRHRLVLSETLGYKIDQKLQGYGEEA
                     LLFELLRASAGERLYLSYQRADAAGRPLAPSGYLDAVGVVPHPSDPDSLFALPRRWPD
                     RVDLPLFTPHLQTREELAVSMVLQGRDVTALLEEVGRDGWLFSHGLGAQGVIESGHPA
                     LSAYDGMLEHAGAHWNTVTERGFSPTALETYARCPFQYFSGQVLKLESVRHVPSMELT
                     PPAMGQLCHDALRFCY"
     gene            complement(9651..9815)
                     /locus_tag="PKK81_04330"
     CDS             complement(9651..9815)
                     /locus_tag="PKK81_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32126.1"
                     /translation="MTVPDDDDFELPILPMIDKGPPPECGTCGDPMKFIDGDWACVDC
                     NGELLGPETG"
     gene            complement(9828..10094)
                     /locus_tag="PKK81_04335"
     CDS             complement(9828..10094)
                     /locus_tag="PKK81_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV32127.1"
                     /translation="MPNITLLHSPTCGACPSAKRLWKELRVKYSFSYREVDITTPDGQ
                     ELANRHSVRAVPATIIDGRLTFVGVPPRQSAEKALQLKMKPQGA"
     gene            10251..11903
                     /gene="pgm"
                     /locus_tag="PKK81_04340"
     CDS             10251..11903
                     /gene="pgm"
                     /locus_tag="PKK81_04340"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HNV32128.1"
                     /translation="MALHPRAGQPAQPEQLVNLEKLEQAYYGEVPDPSDPRQQVSFGT
                     SGHRGSSLRRTFNEAHILAITQAICEYRAQAGTTGPLFIGKDTHALSAPAQRSALEVL
                     AANGIQVRMDQADGYTPTPVISHAILTTNQGRTSGLADGIVITPSHNPPEDGGFKYNP
                     PHGGPADTEVTKAIETRANALLATKLHGVKRVPYDQALKAASTTRHDFVGHYLADLAN
                     VVDLERIKAAKLRLGVDPLGGAAVAYWRPLAERYGLDLHIVNESVDPTFRFMTLDWDG
                     KIRMDCSSPHAMASLIKLKDRFDLAFGNDTDTDRHGIVTPGGGLMNPNHYLAAAISYL
                     FTHRPGWNAGAGIGKTVVSSSMIDRVANDLQRRMVEVPVGFKWFVAGLRDGSLGFGGE
                     ESAGAAFLRRDGATWVTDKDGIIMDLLAAEMLAVTGKDPAQLYADLTARLGEPVYERI
                     DAPATRAQKAALQKFSPQQVQSRELAGEPITAMLTAAPGNMASIGGLKVTTANGWFAA
                     RPSGTEDVYKLYAESFNGQAHLRRIQEDAQALIKQVFSSAGA"
     gene            12099..12797
                     /locus_tag="PKK81_04345"
     CDS             12099..12797
                     /locus_tag="PKK81_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HNV32129.1"
                     /translation="MSRFSHTLCTLLVGAALSACAAPASQGLRQAVTPLPDCCRTTQA
                     DLRKRAIVQTAVNLVGAKTIESQGRRISYDCAGVTRAIYLAHGIDLFEGGAADGKANG
                     VGLIYNHLRKHGQLHRGPVVQAGDLVFFDNTWDYNGDGLVNDPLTHVGIVERVESNGT
                     IVFISRVAGAIERYRMNVAHPHIHRTADGRLLNDYMRRKHWRDGEQTPYLTGELFAAF
                     GTRVVESASSPDRR"
     gene            complement(13014..13379)
                     /locus_tag="PKK81_04350"
     CDS             complement(13014..13379)
                     /locus_tag="PKK81_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32130.1"
                     /translation="MTRYPSRRMAGSAVVLLVVLLIGVTAPVWAEEAGAGSIPAITQI
                     PESRGGVLQQTQAPVAPSLPLLFAEGSNSCEIVSCGMGIKQTCQITCPADKTPKCSCD
                     CERSFGPMCMDYKANCRCE"
     gene            complement(13447..14658)
                     /locus_tag="PKK81_04355"
     CDS             complement(13447..14658)
                     /locus_tag="PKK81_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNV32131.1"
                     /translation="MNGKGLDRRTLLKAAVALPLVLLRPQWLPELRAAEKNGVGAGAG
                     NRERLLLLVELHGGNDGLNTLIPYAEAAYYQARPQLAISRDQVRQLTPQFGLHPALTP
                     LMPFWENKELACLHGVGYPRPNRSHFRSIEIWETASDSEQVLDTGWLSRVFEQHPLPA
                     RFTAEGIVLGKGDSGPLSGGKARTIALHDPGQFLQQAGSVRPASLSTSNPALAHILEV
                     QREISQAASDLQGRIRQAPPLTSDFPANKIGKQLEVAAKLIAAQVPVAVIKVTQGSFD
                     THAGQSATHHRLLEELAQGLTTFRAAMEKLGAWKDVLVMTYSEFGRRVGENASHGTDH
                     GTAAPHFFMGGRVKGGLYGVSPSLRDLQDGDLKHTLDYRAVYATVIEKWWGLPAVSFG
                     TGRYPALDCLT"
     gene            complement(14666..15988)
                     /locus_tag="PKK81_04360"
     CDS             complement(14666..15988)
                     /locus_tag="PKK81_04360"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNV32132.1"
                     /translation="MSLTFDEARHFLSRTAFGGTPEEIRRVMQLDRAAAVAQALAIPT
                     NKAQTTPPAWIHRLPPLPKVRKHWSEAQRKTFREGLKADGQELKAWWYRELLTSRCPL
                     LERMTLWWHNHFTSSLHKVKWPPFLYQQNVLLRLYALGSFRTLLTTIARNPAMLLYLD
                     TQSNRKEHPNENFARELFELFTLGEGHYTEQDIKEAARAFTGWHLDFHTGAFRVDLRH
                     HDDGRKVVFGKSGPFEGDDILLLTLEQPQVARYIVGKLWREFISDRPDSLEVDRLADS
                     FRKKNYEIAGLLQDLLLSQQFWAPENRGVLIKSPVELIVGTARLFSLPIEDTVQQIGL
                     ARRLGQDLFDPPNVKGWPGGTRWITTSSLLDRTQMLHRAIRGHETGHSHGMADMGHVP
                     GRLWLATEPIEVVQATLLPLPPVQPLMEGEERGQAVHQLVLDPVYQLK"
     gene            16130..16561
                     /locus_tag="PKK81_04365"
     CDS             16130..16561
                     /locus_tag="PKK81_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32133.1"
                     /translation="MLRTLSLLILLGLLVVEAGCSTPSGPRLTGAVRYFNIRSEVAPR
                     HLIVQVGDEIRWQNLNSQAVRLRMLEETNPELISCHKGFSQFGVLQDSVTIAPMQSVS
                     LCFSKPTTVRFNVWLEADNPQGAMTPTGSIRIEPSASKRSS"
     gene            complement(16620..16970)
                     /locus_tag="PKK81_04370"
     CDS             complement(16620..16970)
                     /locus_tag="PKK81_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32134.1"
                     /translation="MMLAPSFVTAQTSPPASTPQNQPGQNPQSLGTLPLQNPLAQQSS
                     ITATASGASAPQSNTIPSSQGKSFGTIGKGLPGMTGGPALTAPMGSQDPSSRYMRPTV
                     IPPLFCDPSINIPC"
     gene            complement(17062..17367)
                     /locus_tag="PKK81_04375"
     CDS             complement(17062..17367)
                     /locus_tag="PKK81_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32135.1"
                     /translation="MNMLMLAFRSSLKERVHVLLEQCDVRAYTEMPETTGAGQTGPAD
                     GVSFYPGVNKVIMVAVDDAHAERVERAVQAWRDEVLQRPGGQKPSITVFSWPCRQLV"
     assembly_gap    17593..17692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17693..18399)
                     /locus_tag="PKK81_04380"
     CDS             complement(<17693..18399)
                     /locus_tag="PKK81_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI"
                     /protein_id="HNV32136.1"
                     /translation="MAQAKTKKNGAVGKKLVGMADVVIAAAQRAKDPTFEIPIRALSN
                     VSFNPRRGLIEMGEKKQSRSFFNVGMAKKFMQTMLVADALAELQQADLTTSLREIYYR
                     TKHTIQDSHENTFDTQDESDPIIEDLEVSLVALREELHVRAENSGSIVGPVVFGDDGD
                     RVDCAKLGKGGYSVPSIVEPEYLEIRRCTADFLLLVEKGTQWNRLSEDKFWRRYNCVL
                     LTGNGQPPRGVRRLARRL"
     gene            complement(18408..>20099)
                     /locus_tag="PKK81_04385"
     CDS             complement(18408..>20099)
                     /locus_tag="PKK81_04385"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HNV32137.1"
                     /translation="VFAKLLYGSKFHRMRMSRGQQGIGISAAGMYGQLTTGKPVKVIS
                     RTGPRAAAHYFEVQIDTKKNEPLVHENKQIDWAQPQGTEVALEVEGRYQKGRASVDEW
                     LEQTSIANPHVRLVYHTPEGETKEYPRTYHELPPSPKEIKPHPYGIEFGALLKMLQDT
                     KSHTVAGFLASDFCRVSPALAEEICKAAKVSPATKPRDVKHQAAETLYKTIQTTKIMA
                     PPTNCISPIGEKAILSGLYKQIKGEFYTAVSRPPAVYRGYPFIIEAGLAFGKGPDEAA
                     KPQQAAVPLAEGEDQGNDTEQARVIRYANRVPLLYQQSACATFKAVLSTTWRNYGVSQ
                     SRGALPAGPMVVFVHMASVWVPFTSESKEAIADYDEIQKEITLALREAGRRLGIFLRR
                     RERAASEFRRRNIFDLYIEEVVAACNRLKGGNLPTEKLKEQLHKIASSRTGGLKTDEA
                     LGKTGAGPEGLPHSIIVTAEGIEGETEMAQQTPGSPAPATKTAEAEPITETKNPVKAK
                     RGSKETPKVGKGKSVGATKAKVGQQALFADEQPSTKQSAPLKKKPAQVSATRKRK"
BASE COUNT         3976 a         6401 c         5647 g         3875 t
ORIGIN      
        1 cagccctgaa aaggctggtg tcgacagttc gattctgtcc ctcggcacca taaaatcgat
       61 actctagaaa ctcacggtat gaacgactgc gcgcggccct caactgctcg cgcacttccc
      121 ggtttccctc tcgacttcaa atggtttccg cagcatgaca gtgacaagtc ttcatgtggg
      181 tcactcgatc gagattctct gctcctaaca ccccccgcca ttccctgtgg acgcgaaacg
      241 tttcaagtac ggcacggtca attcccattc aagcatagag accgcaccaa cagtacttag
      301 atttgacaat ggcacgatcg aatgcgcgac ccggccgaca cacaccttag cctcaatcag
      361 tgatcacaat ttgctagcgc gacaggcgac gcgagacact gacctcacga agacccgtcg
      421 gtcgcttctg gtctaacgga tgggacacgg ttgaggggac ctgacaattt ctccgagatt
      481 gttctcggaa accggaaaga gcgggcgccc accatttccc gacagaaatg aaccaggcac
      541 acgagagaga gcatgtatcg gcgttctgcg attggtccga aagtggatcc gacgggagga
      601 gttttcacac tcgcaaggtt ggggaaaact tggaggaacg caaatggggc cggtcgtgga
      661 ggaaaagcgt tggcgttttg gtgcgggagg cgggacttga acccgcacac cttttacagt
      721 acaggatttt aagtcctggg cgtctgccga ttccgccact cccgcagtaa aaactgccgg
      781 ggcgaaatct aacatcgggc tcccgggggg tcaagctgcc cggacgaaca tctgctcaca
      841 tgcaaacggc ctgtccctca ggagacaggc cgctgcagta tgcccctttt gtcagaaccg
      901 gtgccggcaa accaccaggc ttacgcggct tctttatgat cgtccctctt caccaatgcc
      961 ttgctacgtt ccaccatggt ggaaacgccg ctcttcacgg aacgactgaa acgggtggcc
     1021 tggacttgcg cccgcttggc atatcgcgca acatcccgcc tcgtttccgc cccggtcttc
     1081 ggagccagga gtaatcccag cgccgccccc acaactgcgc cccctccgat gatggctgca
     1141 atcttcgctg cttcacgccc cttggtagac atcgtgacat cctcccttta gtaccacaag
     1201 gtttactgtc cactgacttc cggcgagagc taccgatgag tacagcatca accgtgccac
     1261 agccgcacga tgcggattcg ttgggatttc tgcgttacgg cgacaccggg ttaacgactt
     1321 ctcataaagg gtgctgtttt gtgaacaacg gaaggagaca accgtttcag aaaagcgaca
     1381 ggtgtgcgta gggaaaacag ctgaaccggc tgcaggcctt gcccggcgcc gcttcgcgcc
     1441 ctgccttgca ttgcagaaca ggctcacata agatcgcgaa actatgtgga gtcctactat
     1501 gctgtcctgg ttgcacgcca tcccctatcc cgacatcgac ccggtcttcc tgcgcgtagg
     1561 gccgcttcag ttccggtggt acggcctgat gtatctcatc ggcctcacgc tggcctattt
     1621 catcatcgcg gcccgggcca gggcgcagaa gctgccgatg aacaacgatc aggtctacga
     1681 catgatcgtc tatgcagcag tcggcgtctt cgccgggggc cggttgggtt acgtgctgtt
     1741 ctataacctg tcttactacc tggagaatcc gctcaagatt tttgccgtct gggaaggcgg
     1801 catgtcgttt catggcgggc tgatcggcac cattgtggcg ttgatactgt ttgccaggcg
     1861 gcaaggtatg acggttctga ccattgccga cctcgccgcc ggtgtgaccc cggtgggtct
     1921 cggactgggg cgaattggta actttatcaa cggcgaactc tacggccgcc ccacagatgt
     1981 ggactggtgc atggtgttcc cggccggcgg tatggcctgc cgccacccgt cacagctcta
     2041 tgaagcgttc ctggaagggc tcctgctctt taccgtactc tggctgatca cccgacgcct
     2101 gccgccatcg ggcaccgtct tcggcgcctt cctgatgggc tatgggatct gccggattgt
     2161 ggtggagttc tttcgcgagc cggatgcgca gattggattc ctcgtcggta cgatttcgat
     2221 gggacagatg ctcagcattc ccatgatcgt ggcgggagcg gtgatcctgg cgatcgcggc
     2281 ccagcggaaa cgtccgcttc agccggactc aggtacagcc gccccgctct gaagcgtggg
     2341 cggccgttac ggcactgcta cttgggattg gcatccatcc aggattttct gtccaatccg
     2401 agctcgcggt catatttccg aagcggcggg gcatcccaaa tcaccttgtt ccctggagcc
     2461 aggttcgccg cggcgacata gtgtttcctg gcttccgctt tctcgcccaa tcgctccagc
     2521 gttaacgcga gattatagtg ggcttccgcc agcgtctggt cggcctggat cgtcgccaga
     2581 tacacctgct gagccccggc ccagtcctgt tgtgcaaaca actgattgcc ctgctccaac
     2641 tgttgactca ccaccgcgct ggtcccggcc gccgcatgca gcggccgcac ctccgccttg
     2701 tgttgactcg cacagccgac gaccgccatc atgaccaaca ccgcccatcg aaacgccctc
     2761 atgccaccct cctttgcact cagaccgtga ccgccactcc atgtcgcagg aaaataaatt
     2821 caaaacccgc caccggttcg cccgacgact gttcgaccgc cgcccgatac aggcgcgcct
     2881 gttcacgata gagctcagcc cttgccgcca cctggtcggc cgggatcata tccgtcttat
     2941 aatcggcaat ccagagcgcg ccgtcgatcc ggtacagcag atcgatgacc ccttccatga
     3001 tctgacgacc gccgttccat ggcacgagaa acgggacctc gcgcccgatc accgtcgccc
     3061 gccgcagtcg atcgtaggcc gcagattggg cgaaggttcg caacaggtcc cgtacctcct
     3121 cgatcacagc ctcctgcatc gccgtatccg gttcatcgag cgccagcgaa gtgtggtcga
     3181 tcaacgctac ctgtgacgcc acatcggcgg tgaaatccca gtattgcaga agccggtgga
     3241 cgatggtccc cacggtcttc cccggcgtca cccgtctcac ccgccctggt tccctctcgg
     3301 aagccggggc cggtttctga atgaagttcg acggcgagac cagcaaggat tccgatcgct
     3361 gcgcctgcca gttccggtcg cgctgcatcc aacgttcgga gaacgcccgg tccatgaccg
     3421 cgccttctaa tgtgacgggc cgttcgcgct gcctggcggg cggacgatca tcgccctgca
     3481 gaacgatctg acgcaatccg acagcaccga ccggaatgtc ctgctgttcg gccaggccga
     3541 gttccgctcc ggccgcctcc tccaacaatt ccaacaacgc ccctcgcacc cgccgccgcg
     3601 gcagggctcc ggacaacacc agacactccc gtgcgcgcgt catcccgaca tagaacagcc
     3661 gacgccgttc cgcctgttca cgagtacgcg ctttctcggc caccagcacc gcacccaggc
     3721 tacagcgatc ccccaagtcg agaccctgca cgcccgtcga ccagtcatgc caaatgagcg
     3781 gtcgcgcagg accacgcccc gcgccgtctc catggtgtaa cccggccaga atcaccagcg
     3841 ggaactccag gccctttgcc ttatggatgg tgagcacgcg caccgcatcc agcgtgtcct
     3901 ccgagagcgc actctccgct tcttccggtt gctccgtgag ccgatccagc atgagcgaga
     3961 caaagccgtt caacgtgaga ttcggacgat ccgccagatc cgccgccatc tggcgaacct
     4021 tgaaaagatt ggccacggcc tgttctccgt gcagggatgc cgccgcgagt tcgagtaccg
     4081 ggagctgcgc aaaaatgagg tccagcacct caggcaacgg acagcggggc gcctgctcat
     4141 gcagccgcac caaccggccg tacagccgac ggagcggctc ggcatgcgga ctgctccagt
     4201 ctgtcaggcg ctcgacttcg cggtaatcca gcgcctgtcg ttcctgcagg ccgaccaacg
     4261 cggaatcagg cagccccccc actgccgacc ggagcaaccc caccagggcg atgcggtcgt
     4321 gaggattgtc gacgcaccgg agaatgttga ccaggtcgat cacctcctgc cggcggtaaa
     4381 aatgtttctc tccatcgatg atgtaggcaa tgccatgccg ccggagggct tccagatagt
     4441 gctcggcctg agtcaatttt cgaaagagga gcgcgatatg cccgggtcga agcccgcttt
     4501 ccggcccccc cgcttcgccc ccttgcccgg ctggccgcag caagcccgac accatcagtg
     4561 caatctgttc cgcctccact cgcgtggccg ctgccgaatc caagtcgccg tcgtcttccg
     4621 atacgaccaa ccggagttcc acaccggggt tgcgaaactg actcgatcga ttgggttgca
     4681 ccgtcagcgg aacgttcggc ggctgaatgg caggttgcgc caccaggagc cggttgaata
     4741 cgccgttgac cagatcgagc acctgcgcat ggctgcgaaa gttcgtggcc agttcgcagc
     4801 gcaacgcccc gctgccttcc agccgatcga cgacgtggtc gaacgcttcg atatcggccc
     4861 gacggaaggc atagatcgat tgcttcgggt caccaacgat gaacagtttt cccgattcga
     4921 gttccgtctc ccgccatgaa ccggcttgtg tgccggggcg ctccgccaga tagagcacaa
     4981 tctcgtactg caccggatcg gtatcctgaa attcgtcgac cagcagcgcg cgatagtccc
     5041 gtttcaactg ctcacgaatg ggcggatgat cgcgaagcag ggtccgggcc ttgccgacca
     5101 agccgtcgaa ggtgagccag ccggaatcca ggaacgactt ccgcactgat tgcacgaagg
     5161 gcgcgagcag ggccagcaga ttctgcagga gttcatgatc gaccctgagc aaccgtttgg
     5221 cgatccgctg caatgactcg accgcctcgt agtcgtcttc ctcccaaccg gtcggggcgg
     5281 cgccgagatc tttggccaac aattcctgcg gctcgcgtgg gaaagaccgc agcgcgccca
     5341 acccctcggc aagcaggagg gcgaacagct ccgcgactgc ggcgagcatc agttcgattt
     5401 tgcgtcgctt cgggcggtca tatcgtgcga gcagacgttc cgcctgcgcc ttacggaggg
     5461 agaaccagtc acgcaatccc ttattcaggc cggtcccggc cacctgttgc atcagcccat
     5521 tcagatcgat caagtcactg tgcaggctgt aggccaactc gcgcagttcg tccaggctga
     5581 acgtatccag gagtatccgc cagcgaccgt gatcactccc cgccgccccc agctcaccat
     5641 ccagccacaa ctcccactcg ctggtgaagt gttcctcaaa ccgtgaaccg tcttcgtccg
     5701 tttgaaacgt cggtgtgact ccggcttcaa tcgggtacag gcgcaagaga tgcgcggcaa
     5761 aactatgcag cgttccgatc tgcgctttct cgacgtcacg gagtgcggca tcggcccggg
     5821 ccacgatctc atcggtcgtc cagccatacc gcatgcgcag gtcggcgatc gaaaccgtgc
     5881 cactaccggc cgcaggattg tcgcggcctt ccacattcac caacgagcga agccgctctc
     5941 gtaaacggag tttcatctca gtggcggctt tgttggtgaa ggtcagggcc acgatgcgcg
     6001 acagatccaa cggatcggtc cggcgcatga gcaggtacaa caaccggttc accagcagtg
     6061 tggttttccc ggttcccgcc ccggcgatca cgacgacatt cctgtcgaac gtggtggcgg
     6121 ctgcttctct ggccgcctga tccggaatct gcgccggttc agtcacgcgc caccttctgc
     6181 gatcgcacgt cccgtaacgt cctggcctgc gaggaccgat aggcccgcca ccagctcggt
     6241 tgatgggcgc ggcggcaggc ggttgagaat tcacaatggg tgcagtgggc gtccgggacg
     6301 atgaaatatt gtgcggcgcg tatgccatcg agcagcacct gcatcgtcct gctcaacatc
     6361 ggaccggagg gtccctgcca ggccgaagcg gcaaaggaca cacgctcaac cgcaggatcg
     6421 gcatgcggga gcaaatagag aaactcgacc tgctccggta gagctttctc ccggtcaccg
     6481 gacgacccgg caggggtcat taaggcatag agcgcaggct gcagccgttg agcacgcagg
     6541 gccgcttgta ggagattgcg atccttggcc tccacgcggt cgttcgctcg atacttataa
     6601 tcgatgaccc tcaccgcgcc agaccccgga tgccgatcca cccggtccca tcgtccacgc
     6661 aacggaatgt cctccccgcc agaaccatcc ggcaaaagtc cgttggcaac caattcgaat
     6721 tcagcgggat ggaaccccga gaccaacgcc attttccgat cgagtgtgac cgtcgcttcg
     6781 atcaaccgcc ggaccgactc ctgtgccaac tcccaggtca aggcataccc ggtgccatgc
     6841 gtcagagcat aggcttcaaa ggcctgcgcg acagcctgat tcacctccgc aatgatgacg
     6901 gaggcaggca acgtcgtcgc cggccagccc tgttggatca gcgccagata acagaatcgc
     6961 aacgcgtcat ggcagagttg ccccatcgcc ggaggcgtga gtnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnataacaga atcgcaacgc gtcatggcag agttgcccca
     7141 tcgccggagg cgtgagttcc atggagggga cgtggcgcac cgattcaagc ttgagcacct
     7201 gtccggaaaa atattgaaag ggacaacgag catacgtttc cagcgccgtc ggcgaaaacc
     7261 cgcgttccgt gaccgtattc caatgggcgc ctgcatgttc aagcatcccg tcgtacgcgc
     7321 tcagcgccgg atgaccgctt tcaatcactc cttgcgcccc cagtccatgg gaaaataacc
     7381 acccgtcacg tccaacctcc tccaacagag ctgtcacgtc gcgtccctgc agtaccatgc
     7441 tcacagccag ttcctctctc gtctgcaggt ggggcgtgaa gagcggaaga tcgacgcgat
     7501 ccggccaccg acgtggaagg gcgaacagag aatcagggtc cgacgggtgt ggcaccaccc
     7561 cgaccgcatc cagataaccg gagggcgcca agggacgacc ggcggcatcc gcccgttgat
     7621 aggagagata caggcgttcc ccggccgacg ctcgcaacag ctcgaacagc agcgcctcct
     7681 ccccatatcc ctgcagcttc tggtcgatct tgtagcccag ggtttcgctc agcaccaggc
     7741 ggtgccgatc acggagaaat ccatcctcat ggatataccg gggaaacagt ttttcgttca
     7801 ttccgacaag aaagagggca cggaatccca cgccgcgggc cgccatcgca tccaacacct
     7861 gcacgccgtg gtgggaggac ggcgccaggg cacgggacgt tcgcgccaat atctccgcaa
     7921 acgtttctgt ccattcgtcc caggtgaggt taatgtcgag gcgatcgagc gcccgcagct
     7981 gggcgaagac ccgggagagc gcctcattca catccgtggc atcgtcccac tgcgccgtcg
     8041 cgtcaatgga cgtggagact ccgaggggaa cggtcagatg ggtctcagcg agggagcaga
     8101 aggcatcggt cagggtgcca tacccgccct gctcaggaag gcgcttcaca tcgtcgatca
     8161 ggggcgagag gcagccccac agcagacgca actgcacgcc atcgatcgag agcgactcga
     8221 gctcttccgt cacggggtcg tcggcatcgg acgaccagga ttccagacga cccaattgcg
     8281 ccagccgtcg ccactcctcc tcgccgcggg taatcccaag cgccagcacc gccaatcgcc
     8341 agaggtccgg acgcggttca accccgccgc ggttggccgt caggcggcga ttccacgagg
     8401 aggtaatcac ttccaacatg gccggtcgat gcagcccgct tcccttgagc cgggccaggt
     8461 gaagcagcgt cttcacggac ggttcctgta acagcggcac cgtcgcgctg gagagaaacg
     8521 gaatgcgatg ctggtcgaaa gttcgtttca gggacgcctg gtacggcacg agggtgcgac
     8581 ccaccacacc gatttcgtcg aacgcgaagc cgttggtttc caccagcgat aggatttgtt
     8641 tgcagaccag cgtcagctca tcgtcaatcc cggcggcatt tcgtacctca accgatacat
     8701 ttacttcctg agaagcatgc gcaccatcac cggagggagc cccagctcgc tcgtcgaccc
     8761 ctccggcaat cgggtagagg tgccgctcca gaaactgtcg cgcaaaaccg taggccggtc
     8821 gttcgctgag cgggaaatat accgtgaccg gcagggagat gctgagcgat tccaggagag
     8881 tcagctgggt ttgcgtcaga tcgtacgacc cgtagtacca gagaccgttg agactacgga
     8941 gaaacggaga ggcgggtaca aaatcggtga cgagcgcggc caagtcatcc ggagatccca
     9001 cacccagcgc ggcactgccg tcccgcagcg cggcatataa ggtaaacaat cccttaagct
     9061 tctcaccgtc ttcaggcgga aactggcctt cctccactgc gcgtaacgcc agggcagaat
     9121 ccaccgtggc atccttgagg tctcgcacgc tggcccacaa agccggccag gctccggacg
     9181 gcagctgtgg caaacgaagc gccccggtct gaggcacgtt gcgttgcccg aggtgctgaa
     9241 gcaggcgctc gaaaaacgtg tcggtgacga gttcaagctg ccgcaccgca ccagccgctc
     9301 ctgctgtagt gcggcgctcg cgcagcaact gcaacgccaa ctggtgaaac gagagaatgt
     9361 ggacgttcag tagtgccagc ccccgcttga cgaccagcag ccgcttgagc gagcgacgca
     9421 actgatccga gggcaccacc agcgcgactg cggcttgggg gtcgccggac ttgagaacac
     9481 gcagatcgtg aacaagttgc gattcgagtg tgggatgaaa cggaccggtg acgaggcgga
     9541 gcatggagac cgtgggccgt taatcgtaaa gcgcggagcg gaaggccgca gagagtatct
     9601 cggcaaccct ccgcacttca cgcgtgagag gcgaagaacg ccgttgattg ctagccggtt
     9661 tccggaccga gcagctcacc gttgcaatcg acgcaggccc aatcgccatc gatgaacttc
     9721 atcgggtctc cgcacgtccc gcattcaggc ggcggtccct tatcgatcat gggaagaatc
     9781 ggcagttcga aatcgtcatc atcgggaacg gtcatatctc tccaatctca cgcgccctgc
     9841 ggtttcatct tcagctgcaa cgccttctcg gcgctttgac gaggcggcac ccccacgaac
     9901 gtcagacggc cgtcgataat cgtcgcgggc acggcccgga ccgaatgccg attcgccaat
     9961 tcctgaccat ccggggtcgt aatatcgacc tcgcgatagc taaagctata ctttacccta
    10021 agttccttcc acaagcgttt cgctgatgga caggctccgc aggtcggtga gtgcaataag
    10081 gtaatattcg gcatggacaa cccctctcct taacgtaagc ggatcttaac acccgcgcag
    10141 aggaccgcgc aagcaaacgc atgcctgaca ttgctcatcg agccgtatat actgagcgag
    10201 gcatcattca gaaccgcctc atccacccat catcgtttgc tgaggaatct atggctctcc
    10261 accctcgtgc cggacaaccg gcacaaccgg aacaactggt caatctcgag aaacttgaac
    10321 aggcctatta tggcgaagta ccggacccgt ccgatccccg gcagcaggtg agtttcggca
    10381 ccagcggaca tcgcggttcc tcccttcgcc gcaccttcaa tgaagcccac attcttgcga
    10441 tcacccaggc catctgcgaa taccgggctc aggccggaac caccggaccc ctgttcatcg
    10501 gcaaggatac ccacgccctc tccgcaccgg ctcaacgcag tgcactggag gtactggccg
    10561 caaatggtat tcaggtccgc atggatcagg ctgacgggta tacccccact ccggtcatct
    10621 cgcacgcaat tctgaccacg aatcagggcc gcacgtcggg tcttgcggat ggcatcgtca
    10681 tcaccccttc acacaaccct ccggaggacg gcggcttcaa gtacaacccg ccgcacggcg
    10741 gtccggccga cacggaggtc acgaaggcga ttgagacgcg cgccaatgca ttgctggcga
    10801 ccaaactaca cggcgtcaaa cgcgtccctt atgaccaggc gttgaaggcc gcgtccacga
    10861 cgcgacacga tttcgtcggc cactatctcg ccgatctggc caacgtcgtg gatctcgaac
    10921 gcatcaaagc ggcgaagttg cgtctgggcg tcgatccgtt gggcggcgcc gccgtggcct
    10981 actggcgtcc actggcagaa cggtatggat tggacctgca catcgtcaac gagtccgtcg
    11041 atccgacctt tcgtttcatg acgctggatt gggacggcaa gatccgcatg gactgctctt
    11101 ccccccacgc catggcctcg ctcatcaaac tcaaagaccg cttcgatctt gccttcggca
    11161 acgacaccga taccgatcgc catggcattg tgacacccgg gggcggtctg atgaacccca
    11221 atcactacct cgctgccgcg atctcgtatc tgtttaccca tcgcccagga tggaacgccg
    11281 gcgcaggaat cggcaagacc gtcgtcagca gcagcatgat cgaccgggta gcgaatgact
    11341 tacaacgtcg tatggtcgag gtgccggtcg gcttcaagtg gttcgtcgca ggtcttcgag
    11401 acggttcgtt aggattcggc ggtgaagaaa gtgcgggggc ggcatttctc cgtcgtgacg
    11461 gcgccacatg ggtcaccgac aaagacggca tcatcatgga tctgctcgcg gcggaaatgc
    11521 tggcggtcac cggaaaagat ccggcgcaac tctatgcgga cttgaccgcc cgcctcggcg
    11581 agccggtcta cgaacggatc gatgccccgg ccaccagggc gcaaaaggcc gcgctccaga
    11641 aattctcccc gcagcaggtg cagagtcggg aactcgcggg agaaccgatt accgccatgt
    11701 tgacggcagc gcccggcaac atggcttcga tcggagggtt gaaagtgacg acggccaacg
    11761 gctggtttgc cgcgcgcccc agcggaacgg aagacgtcta taaactctat gcggaaagct
    11821 tcaacggcca ggcccatctc aggcggattc aggaagatgc gcaagcgttg atcaaacagg
    11881 tcttctccag cgccggagcc tgaccggatg ccggaatgtt ccgtagaaaa gttcacatga
    11941 gtcgtcaaac gtgagacgtg ggacgacaga acgctctttt tagacacctg caacacgcga
    12001 cgtttcacgt cttacggatt tctggacggt catttccagc ctgcagtgag ttgttgccat
    12061 cgggcatgat tctgaagacc agctatactt aagtcagtat gtccagattc tctcacacgc
    12121 tgtgcaccct gcttgtcggc gccgccctga gcgcctgcgc cgcaccggca tcccagggtc
    12181 tccgccaggc ggttaccccg cttcctgact gctgccgcac aacccaagcc gaccttcgca
    12241 aacgggcgat cgtacaaacc gccgtcaacc tggtcggcgc gaaaacgatc gagagccagg
    12301 gccgccgcat ctcttacgat tgtgccggag tgacccgagc catctacctc gcccacggca
    12361 tcgacctctt tgaaggaggt gctgcagacg gcaaggccaa cggcgtgggt ctgatctata
    12421 accatcttcg caagcatggc caactccatc gcggtccggt cgtacaagcc ggcgatctgg
    12481 tgtttttcga taatacctgg gactacaatg gggacggtct cgtcaacgat cccttgaccc
    12541 atgtgggaat cgtggagagg gtggagagca acggcacgat tgtctttatc agccgcgtgg
    12601 ccggagcgat cgagcgctac cggatgaatg tggcgcatcc tcacattcac cgcaccgccg
    12661 acggccgctt gctcaatgac tacatgcggc ggaagcattg gcgggatggc gaacagacgc
    12721 cctacctgac cggggagtta tttgccgcct tcggcacgag ggtggtagag tcagcctcat
    12781 caccagacag acggtaaaca ccttgtcccc tgctccgttg cgtaccaccc gctgccccga
    12841 atgccaccag cccactacgt ggcaggacaa tccctggcgt ccattctgtt cggagcggtg
    12901 tcaaacgatc gacttggggg cttgggcagg agagcagtac agagtcgcag gacccagcct
    12961 gacggtgggc ggatcggaat catcttcacc ggacaacgag tgaccgggtc ggtctactcg
    13021 cagcgacagt tcgccttgta gtccatacac atcgggccga agctccgctc gcagtcacaa
    13081 ctgcacttgg gcgtcttatc ggccggacag gtgatctgac aggtctgctt gatccccatg
    13141 ccacaggaca cgatctcgca ggaattggag ccttcggcga acagcagcgg caatgacggc
    13201 gccaccggag cctgggtctg ctgaaggacg ccgccacgac tctccggtat ctgggtgatc
    13261 gctggaatgc tccctgctcc agcctcctct gcccaaaccg gcgcagtaac cccgatcagc
    13321 aggacgacca gcagaaccac tgcactcccc gccatgcgtc gcgatggata acgtgtcata
    13381 ccctcacctt cctttcagac ggcagcagaa taggaagaat ggcctgtaaa atcaagtctc
    13441 gactcactaa gtaagacagt cgagcgccgg atagcgcccg gttccgaaag aaacggcagg
    13501 caatccccac catttctcaa tgacggtcgc atacacagcc cgatagtcga gcgtatgctt
    13561 cagatccccg tcctggagat ctctcagtga gggagatact ccatagaggc cgcccttcac
    13621 gcgcccgccc atgaaaaagt gcggggccgc cgtgccgtgg tcggttccat gactggcatt
    13681 ctctcctact cggcgaccga actccgaata ggtcatgacc agcacatcct tccaggcgcc
    13741 cagcttctcc attgccgcgc ggaaggtcgt caggccctgc gccagttcct ctaacaaccg
    13801 gtgatgtgtc gccgactggc cggcatgggt atcgaaactt ccttgcgtca ctttgatgac
    13861 tgcgaccggc acctgcgcgg cgatgagctt ggctgcgacc tccagctgtt tgccgatctt
    13921 attcgccgga aagtcgctcg tgagtggcgg cgcctgccga atacggccct gtagatcgga
    13981 agcggcctgg gagatttccc gctggacctc cagaatatgg gccagagcag ggttgctcgt
    14041 cgagagggaa gccggtcgaa cggacccggc ctgctgcaga aactgccccg gatcatgcag
    14101 ggcaatggtg cgtgccttcc ctccactcaa tggaccgcta tcgcccttgc caagcacaat
    14161 cccctcagcg gtgaatcgcg ccggcagcgg atgttgctca aacacacgcg agagccagcc
    14221 ggtgtccagc acctgctcgc tgtctgacgc cgtctcccaa atctcgatgg atcggaaatg
    14281 ggaacgattc ggtcggggat acccgacccc atgcaggcaa gccagctcct tgttctccca
    14341 gaagggcatc agcggggtca gcgccggatg gagtccgaac tgaggggtga gttggcggac
    14401 ctggtcacgc gaaatcgcta attgcggtcg agcctgataa taggccgcct ctgcgtaggg
    14461 aatgagcgtg ttgagcccgt cgttcccgcc atgcaactca accaacagca acagccgttc
    14521 ccggttgccc gctccggcac caaccccgtt cttctccgct gcccggagtt ccggcaacca
    14581 ctgtggacgc aacagaacga gcggcagagc cactgcggct ttcagcaacg ttcttcgatc
    14641 caaccccttc ccgttcatgg cggcctcact tgagttgata caccggatcc aataccagtt
    14701 ggtgcacggc ctggccgcgc tcttcccctt ccatcaaggg ctgcaccggg gggagcggga
    14761 gcagcgtagc ctgcacgacc tcgatgggct ccgtcgcgag ccacagccgg ccgggcacgt
    14821 ggcccatatc ggccatcccg tgcgaatgac cggtctcgtg gccgcgaatc gcacgatgca
    14881 gcatctgcgt gcggtccagc aggctcgaag ttgtgatcca ccgtgtacca cctggccaac
    14941 ccttcacgtt gggagggtcg aagagatcct gcccgagccg gcgggccaac ccgatctgtt
    15001 gcacggtgtc ttcgatcggt agactgaaga gtcttgcggt gcccacaatg agctcgaccg
    15061 gagacttgat cagtacacca cggttctcgg gtgcccagaa ctgctggctc aggagcaggt
    15121 cctggagcaa tccggcgatc tcatagttct tctttctgaa tgagtcggcc agacgatcca
    15181 cttccagcga gtcgggccgg tctgaaataa attcacgcca gagcttcccg acgatgtacc
    15241 gggccacctg aggctgctcc agcgtcagca agagaatatc atcgccctcg aagggaccgg
    15301 actttccgaa tacgactttg cgtccgtcgt catgatgacg cagatcgaca cggaacgcgc
    15361 cggtatggaa atcgagatgc cacccggtaa atgcccgtgc agcctccttg atgtcctgtt
    15421 cggtatagtg gccttctccc aacgtgaaga gctcgaacaa ctcacgggca aaattttcat
    15481 tgggatgttc tttgcggttg ctctgtgtat cgagatacag aagcatcgcc ggattcctgg
    15541 cgatggtggt gagcagcgtg cgaaacgacc ccagagcata gagccgcagc agaacattct
    15601 gctgatacaa aaacggcggc cacttcacct tgtgcaaact ggacgtaaag tggttgtgcc
    15661 accataacgt catgcgctcc agcagcgggc accgcgaagt gaggagttcc cgataccacc
    15721 aagccttgag ttcctgccca tccgccttca gtccctcgcg gaacgtcttc ctctgcgcct
    15781 cgctccagtg cttacgcacc ttcggcaacg gagggagacg atggatccag gcaggcggcg
    15841 tggtctgagc cttgttcgtg ggaatcgcca acgcctgcgc caccgcggcg gcgcgatcca
    15901 gctgcatcac acgccggatt tcttcaggcg ttccaccgaa cgccgtgcgg gagagaaagt
    15961 ggcgggcctc gtcgaaggtc agactcatgc atgaactctc ggaacaatga acgcggcgct
    16021 ctctctgaca acgactctgt cgcaaccagg ttgacagggt ttcttctcgt cgccctacct
    16081 ggtaacaatc ctagtagcca acgcgcggca tagttctgta gtatcgcgca tgcttcgaac
    16141 gctgtcgctt ctaattttac tcggcctgct cgtcgtagag gccggttgca gcaccccatc
    16201 cggcccgcgc ctcaccggtg cggtccgcta tttcaatatc cgctcggagg tcgcgcctcg
    16261 tcatttgatc gtgcaagtcg gcgatgaaat ccgatggcag aacctgaatt cccaggcagt
    16321 ccggttacgg atgctcgaag aaaccaaccc cgagttgatc tcctgtcaca aagggttttc
    16381 ccaatttgga gtcctacagg actcggtgac catcgctccc atgcaatccg tcagcctctg
    16441 cttctcgaaa cccaccaccg tgcgcttcaa tgtctggctc gaggccgaca atccacaagg
    16501 cgccatgacc cccaccggaa gcatccggat cgaaccatcc gcctcgaaac gcagctccta
    16561 aaaccagcca tcacgcagcc acgtgcgccg gccgaagact ggctatccgc aacggttcgt
    16621 cagcagggaa tattgatgga cggatcgcag aagagcggag gaatcacggt aggccgcatg
    16681 tagcgtgagg acggatcttg cgaccccatc ggcgcggtga gcgccggtcc acccgtcatg
    16741 ccgggcagcc ccttgccgat cgtgccgaac gacttgccct gactggaggg aatggtgtta
    16801 ctttgcggcg cgctggcgcc ggaagccgtg gccgtaatcg acgactgctg cgcgagggga
    16861 ttttggagcg ggagggttcc cagtgattgc gggttttgtc cgggctgatt ctgtggggtc
    16921 gatgccggtg gcgaagtctg ggccgtcacg aacgacgggg ccagcatcat ggccacactg
    16981 aggccgaggg cacagatatt tacgtgaccg agtcttcgat acgatctgcc gtgccgactc
    17041 accgagactc cccttcttca attacaccag ctgcctgcaa ggccagctga ataccgtaat
    17101 ggagggcttc tgtccaccgg gacgttggag cacctcatca cgccacgcct gaacggctcg
    17161 ttcaacccgc tcggcatgcg catcgtccac ggcgaccatg atcaccttgt tgacccccgg
    17221 atagaaggag acaccgtcag caggtcccgt ctgtccggct ccggtcgtct cgggcatttc
    17281 ggtgtaggcg cgaacatcgc actgttcgag taacacatgc acccgttcct tcaatgacga
    17341 ccggaaggcc aacatcaaca tattcatatc gtgactccag gaacgtaacg aggctcgttc
    17401 gacgtgaggc ccggttggag gcaatgcgga gttcctccgc taatccaacc agtccttttc
    17461 cttcagcttg cgcggcagat acttcttcgt caggtattga aagtctttgt tcgcgagcgc
    17521 gtccagttcg tacttcagcc cgttcgcgag catccgtttg atttcctgct gccaggccgg
    17581 cttctggaac cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaggcggcg
    17701 cgccagccgc cgcaccccgc gcggtggctg cccgttcccc gtcagaagca cacagttgta
    17761 tcgccgccag aacttatctt ccgagagccg gttccactgc gtgccttttt caaccagcag
    17821 gagaaaatcg gcggtacagc ggcggatctc cagatattcc ggttcgacga tcgagggcac
    17881 agaatatccg cccttgccca gcttggcaca atccacccgg tcaccgtcgt ccccgaacac
    17941 caccggcccg acgatgctgc cgctgttttc cgcgcgcacg tggagttctt cccggagcgc
    18001 gaccagcgag acttcgagat cctcgataat gggatcggac tcgtcctgtg tatcgaaggt
    18061 attctcatgg gagtcctgaa tcgtatgttt cgtacgataa tagatttccc tgagcgaggt
    18121 ggtcagatcg gcttgttgca actccgccag cgcatcggcc accagcatcg tctgcatgaa
    18181 tttcttcgcc atcccgacgt taaaaaacga gcgggattgc ttcttctcgc ccatctcgat
    18241 cagccccctg cggggattga acgacacgtt cgacaacgcg cggatcggaa tctcaaacgt
    18301 cgggtctttg gcccgttgcg ccgccgcaat gaccacatcg gccatcccga caagcttttt
    18361 ccccacggcc ccgttcttct tcgtctttgc ctgtgccata ccctgtccta tttccttttc
    18421 cgggtggcgg atacctgcgc gggttttttc ttgagcggtg ccgactgttt cgttgacggc
    18481 tgctcatcgg caaacaaggc ttgctgcccg actttcgcct tcgttgcccc gacagacttg
    18541 cccttcccga ccttcggtgt ctccttcgag ccgcgcttcg ccttcaccgg atttttcgtc
    18601 tcggtgatcg gctcagcctc cgccgttttg gtcgcaggcg caggcgatcc aggagtctgc
    18661 tgagccatct cggtctctcc ctcgatcccc tcggcagtca cgatgatcga atgcggcagc
    18721 ccttccggtc ccgcgccggt ctttcccagg gcctcatcgg tcttgaggcc ccccgtccgt
    18781 gaactggcaa tcttgtgcaa ctgttccttc aatttctccg tgggaaggtt tccgcctttc
    18841 aggcgattgc aggcggccac aacctcctcg atatagagat cgaagatatt gcggcgacgg
    18901 aactcactgg cagcccgctc tcttcttcgc agaaaaatcc ccagccgccg gccggcttca
    18961 cgaagcgcga gcgtgatttc cttctgaatt tcgtcgtagt cggcaatagc ttctttcgac
    19021 tcgctggtga acggcaccca cacggatgcc atgtgcacga agaccaccat cggcccggca
    19081 ggcagcgcgc cacgggactg cgacacccca tagtttcgcc aggtggtgct gagcaccgct
    19141 ttgaacgtag cgcacgccga ttgttgatag agcaacggca cccgattcgc gtagcggatc
    19201 acccgcgcct gttcggtatc gttcccctgg tcttcacctt cggccaacgg cacggcggcc
    19261 tgctgcggct tggccgcttc gtccggtcct ttcccgaagg ctaggccggc ctcgatgata
    19321 aagggatagc cgcgatagac ggccggtgga cgactcaccg ccgtataaaa ctcacctttg
    19381 atttgcttat agaggcccga gaggatcgcc ttctccccga tcggcgagat gcagttcgtc
    19441 ggcggcgcca tgatcttggt ggtctgaatc gtcttgtaga gcgtttcggc cgcctgatgc
    19501 ttcacatcgc gaggcttggt tgcgggcgac actttcgccg ccttgcagat ctcctcggcc
    19561 aacgcaggcg aaacccggca gaagtcgctc gcgaggaaac cggctaccgt atggctcttc
    19621 gtgtcctgca acatcttgag caaggccccg aactcgatgc catagggatg cggcttgatt
    19681 tctttcgggg aaggcggcag ttcgtgatag gtccggggat actctttcgt ttccccttcc
    19741 ggcgtgtgat agaccagccg cacatgcggg ttggcgatcg acgtctgctc cagccactca
    19801 tcgacggacg cgcggccctt ctggtaacgc ccttcgacct cgagcgccac ttcggtgcct
    19861 tgcggctgag cccagtcgat ctgcttgttc tcgtgcacca acggctcgtt cttcttcgtg
    19921 tcgatttgca cttcgaaata gtgcgccgct gcgcgaggcc ccgtgcggga aatcactttc
    19981 accggcttgc cggtcgtcaa ttgcccatac atgccggccg ctgaaatgcc gatgccctgt
    20041 tgccctcggc tcatccgcat gcgatggaat ttcgagccgt acagcaattt ggcgaacac
//
LOCUS       DAOQRM010000030        19736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1892_length_19736_cov_12.277476, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000030 DAOQRM010000000
VERSION     DAOQRM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19736
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1892_length_19736_cov_12.277476"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..2574
                     /gene="lptD"
                     /locus_tag="PKK81_04390"
     CDS             217..2574
                     /gene="lptD"
                     /locus_tag="PKK81_04390"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNV32138.1"
                     /translation="MTTASPSNQPLTVTAERIDHLQEQEVYEADGSVVVTQGSFRLTA
                     DHVTINSLPGTLVATGHVHFFDPSSDLKSERLELNVNTEAGVITHGSMYIRPSNSFFT
                     GRSIRRLSEDHYRIKEGTFTNCDAKDGEVPAWRFKFDDLDVNTGESIGMKRAWLCMRD
                     TPIIPVPTLTYPLSNRHSGFLIPTPGYDNRFGLHYQQGYFWAINPSQDLTIAPSYYSD
                     LGYGSDFTYRYYLDRRSSGQWFASFLQQTKLPNVSGVDQTASDERRLRGLISGQHVQQ
                     VTDTLLVRGQASFVSDRQYLQQLSNSGAQRASPSGESTLLATQRLPYGSAYFLGQYLQ
                     PLNSGGPDTFQRLPEVGYVLPNTSAFGLPLLMNLDSNFVNFYREQGFAVNRMDFMPGV
                     TTDVIDVGHVIGLTPHFKFKEVYYTRGIQEASPLHRETFWAAMDASSRLSRRYTGGDG
                     GSFLHTIEPSVMYEYVPGSNQSQIAQIDQVDDIPKKNLLTYSLRTKLLEQQVNGQSFN
                     WLDLTLAQSYHVGGVQTRAREFTPGVLPFLGSLTQPLQPATVEVQGRKLSDLWLRAVI
                     GNTAPEFFRPPGVNEALGKNVTGGQMLPPTNSYLTVDAFLDPYRGTFSQWNTDLRVQQ
                     SNYWYVEVGQRFSRDGNRVRRGDIWNPVSFNEVYAPTEEIQFVTAGGGFRTPWGWTVG
                     AKGYYDVKGGRSPEYDVVALYQNPCKCWSLGLYYLQFPDRQSYSFMLSMTGIGWTENF
                     GTIVARTILGPLLVGDRGLPWAAPGGPYGRTLNPMPVQSMPMGRY"
     gene            2623..3186
                     /locus_tag="PKK81_04395"
     CDS             2623..3186
                     /locus_tag="PKK81_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32139.1"
                     /translation="MRTWWGRRILVRRNRLFLLIGVCLWSAVQGAVPAQLEAAEQPSH
                     LQRAAVFLNAADYRQAVEACQAAVRETPSARTYVFLTYVYHALDGYLEHLATTDQWVR
                     VEQLYLNLATGRSEDLIDPPDVLARIAKEVIQQAVQRQSDVTAAMAARLDEVLVRQFW
                     KEQDAWRRARPTDWWSGAPAEWHWDRP"
     gene            3264..3638
                     /gene="trxA"
                     /locus_tag="PKK81_04400"
     CDS             3264..3638
                     /gene="trxA"
                     /locus_tag="PKK81_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNV32140.1"
                     /translation="MAGRAKEDTVAGDALKATDATWDEDVMKASELVMVDFWAVWCGP
                     CQMVAPIVDELATEYAGKVKVRKLNTDENPEIAGRYQVMSIPTILFFKNGQAVERLVG
                     ARPKRQFKEIIDSLLAQHSGAA"
     gene            3657..5354
                     /gene="recN"
                     /locus_tag="PKK81_04405"
     CDS             3657..5354
                     /gene="recN"
                     /locus_tag="PKK81_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNV32141.1"
                     /translation="MLTELRISNFGVIEQLAVRFGSGFIVFTGETGAGKSLLIDAVTL
                     LVGGRASTDQIRAQSDEADLEAAFVLPSDHPLLHLLQTKEFARPGETDIVIRRVISRT
                     GRNRTYLNGNLCPVHLLEELGGALVDVHGQHEQQSLLSSAAQLEALDAFGRLHALRQD
                     YQVAYRSWQERVAERETLTVHIAQRREREDLLRFQFQEISDAAVEAGEDARLEQERPR
                     LMHSQQLGDLSDQLHELLYAGDQGVLSLLASARKLLAKMVSIDRTAVEWTRVVEDAIV
                     PLRDLADQIRHYRDQVEANPARLMEIEQRLDRLHRLSKKYGGSLDAMLVLQDSLRAQL
                     AQLDTAETRLQDLATAVEDAARRVGELAGRLSRKRIDAAKKLTTQVTKELSALRMERT
                     RFAVEVLPLPGETSYGQMGHDAVEFVFSANPGEPLKPLAKVASGGELSRVMLALKTIL
                     AESDRVPVLIFDEVDAGVGGAVAEVMGTRLRDLSRHHQVLCVTHLPQVGSQAHAHYLV
                     EKQVRQQRTVTQVRLLTEREREEEVARMLAGVTVTNSARAAAAEMIGSAKERRARSD"
     gene            complement(5351..>6521)
                     /locus_tag="PKK81_04410"
     CDS             complement(5351..>6521)
                     /locus_tag="PKK81_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV32142.1"
                     /translation="PAMHPGALWMGTHTSPEYVGTKPSVLVVDDETGPRDALKVILRP
                     FFTIHSADNAKSALRVLKDQHIDLITLDQKLPDRQGIDLLQDIKQDYADIEVIIITGY
                     GSLKSAMEGIRHGAAGYLLKPFNVTELITLINQTLEKKQRLDYLRSFLKTSTALWGTE
                     QEAAQAWKDLQANYFSIGKSAPEPAAKTGDVTALIPLLSDLLEAKDRQLLNHCSRVSF
                     YASLLANQLNLSLPDQKALALGAFLHDIGKISIPNYKYAANDDDSIKNGTLAKAYSEA
                     GARMLLPLEFQAEVGQIVAYHHERFDGLGNPHGLEGEGIPLLARIVAIAQAFDNLTVD
                     MPGHLPMSIDDAIRKIVLQADTHFDPKLTELFAQVVRECKSSLPALAIAKTSPTT"
     assembly_gap    6522..6621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6622..7799)
                     /locus_tag="PKK81_04415"
     CDS             complement(<6622..7799)
                     /locus_tag="PKK81_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV32143.1"
                     /translation="MKKRVLLVDDEPRVRASLRTVLEPTYEILEAADAAEGLKSFKHD
                     APDLVLLDVILPGTDGLAALQTMRTENRAVPVIMLTGTKSVKTAVDAMKLGAADYLSK
                     PFDVEELQIVIERTLGKQELEQEVRQLRAQVVQRYAFHNLIGKSPAMQEIYAKIEQVA
                     DSRTTVLVTGESGTGKELVAKAIHYNSARRERPFVALNCAALPETLIESELFGHEKGS
                     FTDATARRVGQFELAHTGTLFLDEIGDLSAATQAKLLRVLQEREFTRVGGVQSIKVDV
                     RIVAATNKNLDEMVRKNQFREDLYYRINVIALYLPPLRERGEDIALLAKHFLAKRIEE
                     EKRPPQEFTKSSLELISHYPWPGNVREMENIIEQAFIWSKGSDVITPEHLPNILRTDT
                     R"
     gene            complement(7801..8736)
                     /locus_tag="PKK81_04420"
     CDS             complement(7801..8736)
                     /locus_tag="PKK81_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32144.1"
                     /translation="MMSIQAAVCIPLRNKTQFLGLCALGPIGTECHLDEDTVIIAETV
                     ARIACNTLDHHLAQDDLRRSSTLMRRTDRLRSLEIMAGGFAHEIRNPLTSIKTFIQLA
                     PQRQQDPVFIREFSQVAIEDVHRIERLLHEILDYASYMTPQPSDVDLNELVTSCLGFV
                     SSTASHRGIRLHTTLAPQLPTLSLDRQQIKQVLLNLLLNALDAMPNGNGVIQIRTRVL
                     PQAGRTAWVQLEVEDEGCGIAPDHLEHIFDPFFTTKHTNSASDGSGLGLTTAHQIVRE
                     HGGTLLVESRVGAGSTFSVNLPAPDARTTVSAARE"
     gene            complement(9188..13393)
                     /locus_tag="PKK81_04425"
     CDS             complement(9188..13393)
                     /locus_tag="PKK81_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV32145.1"
                     /translation="MKSSMSPAEPSLNILLINEQPDEIKLVTSSLRGFFPGCRIEAGY
                     SSEEALTFSHQGEWHIILIDHDLRPERGLDILSRLRRNAPYAAIILQTNESDSQTAVQ
                     ALQNGADFLLFKRSPAFITELLFSVQEAVEKRDLQMKLDHTFQRHQRFIETLSDLVYE
                     LDRDGRFVYVGTAVTAMLGYTPEELAGRHYSLLLPPLQEATGRFRLNERRAGSRSVRR
                     LELTLHRKALPDAPPASITVDVTAKGLFDSANRYLGTVGVLRDLSQQKAQQDRLTELE
                     SRLQETTRQLTLSREAARVSRQLQQPLTTLLQDSQRLLSSIQHSKIEQHVETMIEKAS
                     QASQLGHQLAQAIHAPPLEFFPLDLHHVLETVVQTIRHETEGSGRLVTTHLAQHLPLI
                     LGSPDALEDLVRILLNYAHQYTSLPNAPSHLLLQTALLAGVTPSLASRNLPLYSSVSR
                     EYVSFTIKESSNHTAAPMGEQPDFHISPEQFFRAHQIVQAHGGAIEIEHAPDSGLTIT
                     VRIPVPTHFFADEGAESPTASPAKPGIVRVQSQHVAGERPDRRRSERKLFALPVQLSV
                     GSTTLRGVLRNMSTGGALLTIHDLSASIHLQPAYVVIKTPVSFLELQGVVHERPAALT
                     DLPLPAIKNFVISFSPSGERDRNVLRSLLDGLQDGSTIVTFEGLILPLFTTAANNPDA
                     PLPSTAAPTDRREAARLNVPCPIRLVGSSATPTRPLGVTVNLSLDGACIGLPGGSDFP
                     ENQQVLHLVPVEPNTSSIGAALPEGSETPWPARIVWTHQSQVRTTSNPAAPEGVRRRF
                     GVRFEGLSPAQEQRLRALISPRIGTSQDLAEPMSDSPVLTVSHHLRNREGLTIALCHD
                     VPRRVDGTQCPLVLLCSGYGTTQQAYAALAYTLAACGLQVLRYDHSRHIGLSDGDPTQ
                     TTLTTLEDDLDTILSFAQKEFPGLPLTLLAPDLLGRIALRRQDWHRSVRRLLLLNPTL
                     DIRNCLTSLHQRDLVGEHLAGTRLGLGNLLGIPLDIDHFVADAVAAQYTESNALQEDL
                     SHCGTEVVFLTTGPGAAEYPTPSPAPALVEDALHRLGSRGSRVMLQAPLLPADTVAPS
                     ALHASWHRIAQLCLTPDSFPYPTAPAPSNLSRMISIRARLERDRLRIKYAAGTAGRER
                     LWAAQTDLTRSLDELPAHWQYIDQLYQLLQPLDGGVALLDVGCGIHSFARLLLLNLSY
                     RLRAQTWNQNQPLRYIGIDFSVAALPAAQDATSDALKHVDRLFSGRISGPTPVSQQWV
                     RGRSVETLPFADHSFDRIVANLSLSFSRSPLHALRELFRVLRPGGKLIVSAITPLADM
                     ALPYRSSLQELGVDAFSGDARLALNRMAQCCVALRVGQLHAFQEESLSAALAQVTSTP
                     TRLVRTFSGQILVALAEKPDSAG"
     gene            complement(13784..14785)
                     /locus_tag="PKK81_04430"
     CDS             complement(13784..14785)
                     /locus_tag="PKK81_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32146.1"
                     /translation="MNRALLSMVMLLMVTSTVYADGGHDHHAIPTMPSLGDTKITIDD
                     HTVTLTFGPVDLPTGHDGDLASSMPKKVFQLPDDTYMIGYKSEVYTKDGKPLPKNYLH
                     HILMLNNDKPSVSCDGEPLFFAGAGLEMTEARFPDGYGVKLGKGQNLMSVVAFYHKAP
                     PTKDVMARFTMYVAPKGTKVQEMDVYQVGVNIVCYSKFGQRGADQTDEGIEIKPGVSV
                     LSAPLKFNMDGCVKFAYPHGHDELLMVALENKSSRETLLRTVPEVGADGTFRDFRPHQ
                     VYRDDKGFTVTKRDDYEMVMVHHHPLQNPNIQHGMGNYLLYMTPGVCPQSANTASAR"
     gene            complement(14859..15698)
                     /locus_tag="PKK81_04435"
     CDS             complement(14859..15698)
                     /locus_tag="PKK81_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNV32147.1"
                     /translation="MSILAGSLAAAKEPVDAEATLRLIVQANADRDLPTMSKYMSHDS
                     DAVGYTIDGHKYVGWTALATDMRTEFTSVAKLELPITYLQFWHKGDIAWFAMELDYIR
                     YLNATDLTQRMVLPLRETGVMERRDGTWILVTWHESTRQPDTGMGIALADPGTLVRRV
                     SDNQAPGPNGADLSGEWEVTEIEDNKKYVATLDAHGNGPYTWQGGQFATTSFNDRRWQ
                     GTWKQTGNDREGAFEVVISEDGTEAKGIWWYVRVGTRDNIPPRQHGGTYLWKRLTPPP
                     TAP"
     gene            complement(15857..16663)
                     /locus_tag="PKK81_04440"
     CDS             complement(15857..16663)
                     /locus_tag="PKK81_04440"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNV32148.1"
                     /translation="MSTQQNRWAWRAFGQTERGLVRPTNQDTFLVENRHRLWAVADGM
                     GGHPGGDVASRLVMDSLAELAPTLGGIASAPDLHEEERATDRLTTMAEQAHASILTHA
                     AQHPPLYGMGTTLVMAHLLPLPKPRLLVLNVGDSRAYLVRAGTLTQLTRDHTLIEEYV
                     RDGRLTPAQAACHPNRHVLTRAMGLAPHLESDLFFFDLLDGDLLLLCSDGLTKMLTDE
                     RILQTVRPHQHIPSLATQALIHTALDAGGIDNVTVVACTITEIPDQANIH"
     gene            complement(16665..17438)
                     /locus_tag="PKK81_04445"
     CDS             complement(16665..17438)
                     /locus_tag="PKK81_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNV32149.1"
                     /translation="MLTSTFVHLNGIGPSTERRLWEGGIADWTTFRREPNLPGISPSR
                     KVLYDIDLASAQEQLDRRNARYFAGCLHTRDHWRLFRTFGPRALYLDIETTGLSAHEG
                     QVTIVGLYRQGRLRTLISGDDLTLGVIQEELDQADLLITFFGSVFDIPYLQTCFKGLR
                     VSIPHFDLCFAARRVGLQGGLKHIERELDIARDSDLLTLDGMEAVRLWHQYRAGDEAA
                     LDQLVRYNAADTRNLEPLAEWLYDRLALRYGPPSLTPAG"
     gene            17571..17981
                     /locus_tag="PKK81_04450"
     CDS             17571..17981
                     /locus_tag="PKK81_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HNV32150.1"
                     /translation="MTTNGSAKRIRIKVGGIQLEAELKGTRTAEELYRALPAEGPLNV
                     WGEEFYFKIPGVKDHRETATTQVKVGDVAFWGAGQVLAIFFGRTPMSMGADPVPADRV
                     NVVGRVVGDAATLRQAMNASTIRVERIEQGSSIG"
     gene            18039..18668
                     /locus_tag="PKK81_04455"
                     /pseudo
     CDS             18039..18668
                     /locus_tag="PKK81_04455"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
     gene            18704..19144
                     /locus_tag="PKK81_04460"
     CDS             18704..19144
                     /locus_tag="PKK81_04460"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32151.1"
                     /translation="MRVALWIVFCVMAVSLPACTHSKPQPLVPLELAYGAKADAIALN
                     NQGMQAYLAGQVAEAKNFFGQAIKASPDSGQAHYNYALALNALGQTDQARLEFIEAAN
                     LAPGDKVIWDSPALRPFGSPQAQKGPPREHPYGTQRPVIGSGPR"
     gene            19184..19651
                     /gene="bcp"
                     /locus_tag="PKK81_04465"
     CDS             19184..19651
                     /gene="bcp"
                     /locus_tag="PKK81_04465"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNV32152.1"
                     /translation="MADELDVGAKAPDFSLPDQDGSTVTLKSLKGKQVVLYFYPKDDT
                     SGCTKEACDFRDSLAPIKKAGAVVIGVSKDGKASHQKFIAKYGLPFALLSDEEAEVCK
                     AYGVYKEKSMYGRKYLGIERSTFVIDATGRIKALFRKVKVPGHVDEVLAALKA"
BASE COUNT         3900 a         5141 c         6419 g         4176 t
ORIGIN      
        1 atctgatttg tgtcacgggc tccttgatgc tggtcggcga gatcaaggcc cttctccgag
       61 ggtgttccct ctcacctgtt cgtggttgag atggggccag accgctactc ggcttccata
      121 cggcggcgga cggctggttg tccgttcccg gttgtcctcc ttactctcct cctcagtgtg
      181 ttgctgctgg tcgaagccgc ctcggcgcaa tcaacggtga ccaccgcctc accgagcaac
      241 cagcccctca ccgtgacggc cgaacgcatc gaccatctgc aggagcagga ggtgtacgaa
      301 gcggacgggt cggtggtggt gacgcaaggc tcgtttcgac tcacggccga tcatgtcacg
      361 atcaattcgc tgccgggtac cttagtggcg accgggcatg tgcatttctt cgacccgagt
      421 tccgatctga agtcggagcg gctggagttg aacgtgaata ccgaagccgg agtcatcacc
      481 catgggtcga tgtatatccg gccaagcaac tcgtttttta ccggtcgcag catccggagg
      541 ctgtccgagg atcactatcg catcaaggag gggacgttca cgaattgtga cgccaaagac
      601 ggcgaagtgc cggcctggcg attcaagttc gacgatctcg acgtgaacac gggcgaaagt
      661 atcgggatga agcgggcgtg gctctgcatg cgggatacgc ccatcatccc tgtgccgacc
      721 ctgacctatc cgctcagtaa tcgtcacagc gggttcctca ttcccacgcc cggctacgac
      781 aaccgcttcg ggctgcacta ccagcagggc tatttctggg ccattaaccc cagccaggac
      841 ctgacgatcg caccttcgta ttattcggat cttgggtacg gaagcgattt tacctatcgc
      901 tattatctgg accggcggtc gagcgggcag tggtttgcca gctttctgca gcagacgaaa
      961 ctgccgaatg tgtcgggcgt cgatcagacc gcctcggatg agcggcgctt gcgaggcttg
     1021 atcagcggcc agcatgttca acaggtcacg gacacactcc tggtgcgagg ccaggcgtcg
     1081 tttgtgtcgg atcggcagta tctccaacag ttgagcaact caggagcgca gcgggcctct
     1141 cccagcggcg aatccacatt actggcgacg caacgcctgc cctacggcag cgcctatttt
     1201 ctgggtcagt atctccagcc gttgaactcc ggcggtcccg acacgttcca gcgcctcccg
     1261 gaggtcggct atgtgttgcc gaatacgtcg gcgttcgggc tgccgctgct catgaatctc
     1321 gacagtaact ttgtgaattt ttatcgcgag cagggattcg cggtgaatcg catggatttt
     1381 atgcctggcg tcaccaccga tgtgatcgat gtcgggcatg tgatcgggtt gacgccgcat
     1441 tttaaattca aggaagtcta ttatacgcgc ggcattcagg aggccagtcc tctccatcgc
     1501 gagacgtttt gggcggctat ggatgcgtct tccaggctga gccgccggta taccgggggt
     1561 gacggcggga gtttcctgca cacgatcgaa ccgagtgtga tgtatgaata cgtgccgggg
     1621 agtaaccagt ctcagatcgc ccagatcgat caagtcgatg acatcccgaa gaaaaactta
     1681 ttaacctatt cactccggac caagttgctc gaacaacagg tgaacggcca atcgttcaac
     1741 tggctcgact tgaccctggc gcagagttat cacgtgggcg gggtgcagac ccgcgcgcgg
     1801 gagtttactc ccggcgtgtt accgttcctg ggatctctca cgcagccctt gcagccggcg
     1861 accgtggagg tgcaggggcg aaaactgtcc gacttgtggc tacgggcggt gatcggcaat
     1921 accgcgccgg aattctttcg tccacccggt gtcaacgaag cgctcggaaa gaatgtgacg
     1981 ggcggacaga tgttacctcc gacgaattcg tatctgacgg tcgatgcctt tctcgatccc
     2041 taccggggga cgttcagcca gtggaatacc gatcttcggg tgcagcaatc caactattgg
     2101 tacgtggagg tcgggcaacg gttcagtcgt gacggcaacc gggtccggcg cggcgatatt
     2161 tggaatccgg tctcgtttaa tgaggtctat gctccaaccg aggagatcca gttcgtcacc
     2221 gcgggcggcg gatttagaac accgtggggc tggaccgtcg gcgccaaggg ctattacgat
     2281 gtcaaaggcg gacgcagtcc ggaatacgat gtggtcgcct tgtatcagaa tccgtgcaag
     2341 tgctggtcgc tcgggttgta ctatctgcag ttcccggacc gccagagtta ctccttcatg
     2401 ctgagcatga ccggcatcgg ctggacagag aacttcggca cgattgtggc gcgcacgatt
     2461 ttgggtccat tgctggtcgg ggatcgcggc ttgccctggg ctgcaccggg tggcccctat
     2521 ggacgaaccc tgaatccaat gccggtgcag tcgatgccga tgggtcggta ttgagccgcg
     2581 gaatctcgcc tgactggtcg agattggacg gcctggagcg cgatgcggac atggtggggg
     2641 aggcgtatcc tcgtgcgacg aaaccggctc ttcctgttga tcggggtatg cttgtggagc
     2701 gcggttcagg gggcggtccc ggctcagttg gaggcggcgg agcagccatc gcatcttcaa
     2761 cgcgctgcgg tgtttctcaa cgccgctgac taccgtcaag cggtggaggc ctgtcaggcg
     2821 gcggtgcggg agacgccttc ggctcgaacc tatgtctttc ttacgtatgt gtatcacgcg
     2881 ctcgatggct accttgaaca cctggcgacc accgatcaat gggttcgcgt ggaacagctc
     2941 tacctgaatc tcgcgaccgg acggagtgaa gatctgattg atccgccgga tgtgctcgcg
     3001 cggatcgcaa aagaagtgat ccagcaggcg gtgcagcgac agtctgacgt cactgcggcg
     3061 atggcggctc gcctggatga ggtcctggtg aggcagttct ggaaggaaca ggatgcgtgg
     3121 cgacgcgcca ggccgacgga ctggtggtcg ggtgcgccag ctgagtggca ctgggacagg
     3181 ccgtgatttg actctctgca aaaccgtgtt ataggatgac ggacggtgtg tggaaccgct
     3241 tcagcacatc gaaaaacgat gatatggcag gtcgagcgaa ggaggacact gtggcaggcg
     3301 atgcgttgaa ggcgactgat gcgacgtggg atgaggatgt catgaaggca tccgaattag
     3361 tgatggtgga tttttgggcg gtctggtgcg ggccttgtca gatggtcgcg ccgatcgtcg
     3421 atgaattagc aacagaatat gccggcaagg tcaaggtccg taagctcaat accgatgaga
     3481 acccggaaat tgccggacgc tatcaggtga tgagcattcc gaccattctc ttcttcaaga
     3541 acggtcaagc ggtcgagcgg ctggtggggg cgcgtcccaa gcgccagttt aaagaaatca
     3601 tcgattcgct tctcgcgcaa cattccggcg ctgcctagcg cctgatcctc agcggcatgc
     3661 tgaccgagct gcgcatctcg aatttcggcg tgatcgagca gctggccgtc cgtttcggct
     3721 ccggcttcat cgtgtttacc ggtgaaaccg gagccggcaa atctctcctc atcgatgcgg
     3781 tgacgctcct ggtcgggggc cgtgcctcga cggatcagat ccgtgcccag tccgatgaag
     3841 ccgatctcga agcggcgttt gtgttgccgt cagatcatcc ccttctccat ctgctccaga
     3901 ccaaggaatt tgcccgtccg ggtgagacgg acattgtcat ccgtcgcgtg atttccagga
     3961 cgggccgcaa ccggacctat ctcaacggca atctctgccc ggttcatctt ctggaggaac
     4021 tgggcggcgc gttggtggat gtgcatggcc agcacgagca gcaatcgctg ttgtcctccg
     4081 cggctcagtt ggaggcgttg gatgccttcg gccgacttca tgctcttcgt caggactatc
     4141 aggtcgcata tcggtcgtgg caggagcggg tggctgaacg tgagacactg actgtgcaca
     4201 tcgcccaacg ccgagaacga gaggatctgc tgcgttttca atttcaggaa atctccgatg
     4261 ccgcagtgga ggcgggggag gacgcgaggc ttgaacagga acgcccgcgt ctgatgcata
     4321 gtcaacaact gggcgacctc tccgaccaac tccatgagtt gctgtatgcg ggtgatcagg
     4381 gcgtgctgag cctgttggct tccgcgcgca aactgctcgc gaagatggtg tcgatcgatc
     4441 ggactgccgt cgaatggacg cgggtagtag aggacgccat cgttccgctg agggacctgg
     4501 ccgaccagat ccggcactat cgtgatcaag tcgaagccaa cccggcacga ttgatggaaa
     4561 tcgagcagcg gctggatcga ttgcatcggc tgagcaagaa atacggcgga tcgttggatg
     4621 ccatgctggt gctacaagac tctctacgcg ctcagctggc ccaactggac acagcggaaa
     4681 cccggctgca ggacctggcc actgccgtgg aggatgctgc acgtcgcgtg ggtgaactgg
     4741 ccgggcgtct ctctcgcaag cgaatcgacg cggccaagaa actcacgacg caggtgacga
     4801 aagagttgag cgcgcttcga atggagcgaa cccgcttcgc ggtggaggtg cttccgctgc
     4861 ccggcgagac gtcctacggc cagatgggcc acgatgccgt ggagttcgtc ttttcggcca
     4921 atccgggtga accactcaag ccgcttgcca aagtggcttc cgggggggaa ctgtcccgtg
     4981 tcatgttggc gttgaaaacc attctggccg agagcgatcg agttcccgtg ctgatttttg
     5041 acgaagtcga tgccggagtc ggcggggccg tggctgaggt catggggaca cgtctccggg
     5101 atctcagccg gcaccaccag gtgctctgtg tgacgcatct tccccaggtc gggtcccaag
     5161 cccatgcgca ttacctggta gagaagcagg tgcgccagca gcgcaccgtg acgcaagtcc
     5221 ggttgctgac ggagcgcgag cgggaagagg aagtggcgcg gatgttagcc ggggtcacgg
     5281 tgaccaacag tgcgcgcgcg gcggcggctg aaatgatcgg cagcgcgaag gaacgacggg
     5341 cgcgatcgga ttaggtcgtc ggtgaggttt ttgcgatcgc gagggcgggc agggaagatt
     5401 tgcattcccg cacgacttgg gcaaaaagct cggtcagttt cggatcgaag tgagtgtccg
     5461 cttggagcac gatcttgcgg atggcgtcgt cgatggacat cggaaggtgt cccggcatat
     5521 cgaccgtcag gttgtcgaaa gcttgggcga tggcgacgat gcgtgccagg agcggaatgc
     5581 cttcgccctc cagcccgtga ggattgccca gtccgtcgaa tcgttcatgg tgataggcca
     5641 cgatctgtcc cacctcggcc tgaaattcaa gcggtagcag catccgcgcg ccggcctcag
     5701 agtatgcttt ggcgagggtc ccgttcttga tactgtcgtc atcgttcgct gcatacttat
     5761 agttgggaat actgatcttc ccgatgtcgt ggagaaacgc gcccagtgcg agcgccttct
     5821 gatcgggaag ggagaggttg agctggttgg caagcagcga ggcataaaag ctgacgcggc
     5881 tgcagtggtt caggagttgg cggtcctttg cttcgaggag gtcggataac aacgggatca
     5941 acgcagtgac atcgcctgtt ttggcggccg gctcgggggc ggatttgccg atggagaaat
     6001 agttggcttg cagatctttc cacgcctggg ccgcttcctg ttccgttccc cacaaggcgg
     6061 tcgatgtttt gaggaacgag cgcaggtagt ccagccgctg ctttttctcg agtgtttggt
     6121 tgatgagggt gatcagttcg gtcacgttaa atggcttgag cagatatccc gccgcgccgt
     6181 gccggatgcc ttccatcgca gacttcaaac tgccatagcc ggtaatgatg atgacttcga
     6241 tgtcggcgta gtcctgcttg atgtcttgca gcagatcgat tccctggcgg tcgggtagtt
     6301 tttgatcgag ggtgatgagg tcgatatgct ggtctttgag gacccgcagg gctgatttgg
     6361 cgttgtcggc ggagtggatg gtgaagaacg ggcgaaggat gactttgagc gcgtcccgtg
     6421 gaccagtttc atcatcaacg accagtaccg acggtttcgt acctacgtat tcaggtgatg
     6481 tatgggtccc catccagagc gctcccgggt gcattgcagg cnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn ncgtgtatcg gtgcgtagga tattcggtaa gtgctccggc
     6661 gtgatgacat cggagccttt cgaccagatg aacgcctgct cgatgatgtt ctccatctcg
     6721 cggacgtttc cgggccaggg atagtgcgag atcaactcca gcgagctttt cgtaaattcc
     6781 tgaggaggac gtttctcttc ttcgatacgt tttgccagaa agtgtttggc caggagggcg
     6841 atgtcttcgc cccgctcgcg caggggcggt agatacagcg cgatgacgtt gatgcggtaa
     6901 tacaggtcct cgcggaactg gttcttccgg accatctcat cgaggttttt gttggtggcg
     6961 gcgacgattc ggacatccac cttgatggac tgcactccgc ccacgcgggt gaactcacgt
     7021 tcctgtagca cgcggaggag tttggcctgg gtcgccgcgc tgaggtcccc gatttcatcg
     7081 agaaaaagcg tgcccgtatg cgccaattcg aactggccta cgcgccttgc cgtggcatcg
     7141 gtaaaggagc ctttttcgtg gccgaacagt tcgctttcga tcagagtttc aggcagagcc
     7201 gcacaattga gcgccacgaa cgggcgttcg cgccgcgcgc tgttgtaatg aatggccttg
     7261 gcgacgagtt cttttccggt cccgctctcg ccggttacga ggacggtggt gcgactgtcg
     7321 gccacttgct cgatcttcgc atagatttcc tgcatcgccg ggcttttgcc gatcagattg
     7381 tgaaaggcat accgctggac gacctgcgcg cgcagttgac gaacctcttg ttctaattcc
     7441 tgtttgccca gcgtgcgctc aatgacgatt tgcagctctt cgacatcgaa tggcttggat
     7501 agatagtcgg cagcccccag tttcatggca tcgacggccg tcttgaccga tttggtcccg
     7561 gttagcatga tcaccgggac ggcacgattc tcggtgcgca ttgtctggag cgccgccaga
     7621 ccgtctgttc ccgggaggat cacgtccagc aggaccaggt ccggcgcatc gtgtttgaaa
     7681 ctcttgaggc cttccgcggc gtccgccgct tcgagaatct cataggtcgg ttccagcact
     7741 gttctcagcg aggcgcggac acgcggctcg tcatcgacaa gtagtacccg ttttttcatg
     7801 ttactcccgt gcggctgaaa ctgtggtgcg tgcatccggt gctggaagat tcacggaaaa
     7861 cgtcgaacct gcgccgactc gactctccac caatagcgtg ccgccatgtt cgcggacgat
     7921 ttgatgcgcg gtggtcaggc caaggccgga cccatcgctc gcgctattgg tatgcttggt
     7981 tgtaaaaaac ggatcgaaaa tatgctccag gtggtccggg gcaatgccgc atccctcgtc
     8041 ctcgacctcc agttgtaccc aggctgtgcg gccggcttgc ggcagcacgc gcgtgcggat
     8101 ctgaatcacg ccgttgccgt tgggcatggc atcgagcgca ttcaatagca ggttcaagag
     8161 cacctgtttg atctgctggc gatcgagcga gagcgtgggc agttgagggg ccagtgtcgt
     8221 gtgcagacga attcctcgat gcgaggcggt ggacgagacg aaaccgaggc aggaggtgac
     8281 cagttcgttc aggtccacgt cgctcggctg gggagtcatg taactcgcat agtcgaggat
     8341 ttcgtgcagc agccgctcga tgcgatggac gtcttcaatg gcgacttggc tgaattcacg
     8401 gatgaacacc gggtcctgct ggcgttgcgg ggccagctga ataaacgtct tgatcgacgt
     8461 gagggggtta cggatttcat gtgcgaaccc gcccgccata atttccaacg agcgtaatcg
     8521 atcggtgcgt ctcatcaagg tggaggagcg ccgaaggtcg tcctgcgcca ggtgatggtc
     8581 caaggtattg caagcgatgc gtgcgacggt ctctgcaatg atcacggtgt cttcgtccag
     8641 gtgacactcc gtgccgatcg gcccaagggc gcagagcccg agaaactgcg tcttgttccg
     8701 gagaggaatg cacaccgctg cctgtatcga catcatggct gagccggcgg gcgaagatga
     8761 ggcctggtcg tatcggagta ggcgtggccg atctgaaaga cacgtgataa gggcgtggtt
     8821 atccggcagg ctagccggca atgccgcaag cgtgcggtgt cctagagagg caaggagatg
     8881 gtattcgccg tttgcttctc gtatccatat ggcggctttc ggcaagcgca actgttcgct
     8941 gaggatcctc aagatgcact cgccgacctg cgtgaggtcg gtggcctgtc ccaccgattc
     9001 ggcaaacgcg ccgatcatac tgatgggggc ttcggaagca gaaagaatag gggtgtgccg
     9061 aatgtgctcg tggagtgcca tagtcttgcg cagaaaatat caacccggac accatcgtgc
     9121 atgtggtgtc gtccgacgcg ccgcatgtcg tgtgttagtg actgaagtat atatgcgagg
     9181 tattgtttca gccagcggaa tcaggttttt cagcgagggc gaccagaatt tgtccggaga
     9241 acgtacgcac gagtcttgtt ggagtagatg tgacttgggc gagggcggcg ctcaggctct
     9301 cttcctggaa tgcgtggagt tgaccgacgc gcagggcgac gcaacactga gccatgcgat
     9361 tgagggcaag gcgggcgtct cccgagaagg catcgacgcc gagctcctgc aaggatgaac
     9421 gataggggag ggccatatct gccaaggggg tgatggcgct cacgatgagt tttccgccgg
     9481 ggcgaagcac acggaacagc tcacgaagag catggagagg agaacgtgaa aagctgaggg
     9541 agagattcgc cacgatgcga tcgaacgaat ggtccgcgaa gggaagcgtt tctacggatc
     9601 gaccacgtac ccactgctga ctgacgggtg tcggcccgga gatgcgtccg gagaacagcc
     9661 gatccacgtg cttcagggcg tcactcgtcg cgtcctgcgc ggcaggcagc gcggccactg
     9721 agaagtctat gccgatgtat cgaagaggct ggttctggtt ccaggtttga gcgcggagac
     9781 ggtaggagag gttcaagagc aagagtctgg cgaatgaatg aatgccacag ccgacatcga
     9841 gcagagccac gcctccatcg agcggttgga ggagttgata cagctgatcg atatattgcc
     9901 aatgcgcggg gagttcatcc aaacttcgtg tgaggtcggt ctgtgcggcc cagagtcgct
     9961 cgcggcctgc cgttccggcg gcatatttga tgcggagtcg gtcgcgttcg agtcgtgcgc
    10021 gaatggatat catgcgtgag agattcgagg gagccggggc ggtgggatac gggaagctat
    10081 ccggagtaag gcagagctgt gcgattcggt gccagcttgc gtgcagtgcc gatggtgcga
    10141 cagtgtcggc aggcagaagc ggagcttgta gcatgacgcg actacctctt gatccgagcc
    10201 gatgcagagc atcctccacg agtgccggcg ccggtgaggg agtcgggtat tcagcagcgc
    10261 ctgggccggt cgtcagaaac acgacctcgg ttccacaatg cgagaggtcc tcctgaagcg
    10321 cgttgctctc tgtatattga gcggccaccg catcggccac gaaatgatcg atgtcgagtg
    10381 gaatccccag gaggttgccg agcccgagcc gcgttccggc cagatgttca cctaccagat
    10441 cccgctgatg aaggcttgtc aggcagtttc ggatgtcgag tgtcggattg agaagtaaga
    10501 gccggcggac tgacctgtgc caatcctggc ggcgtagcgc gatgcggccg agcaggtccg
    10561 gtgccagcaa cgtgaggggg agtccgggga actctttctg cgcaaagctc agtatggtat
    10621 cgagatcatc ttccagggtt gtgagcgtgg tttgcgtggg gtcgccgtcg ctcaatccga
    10681 tgtgacggct gtgatcatag cgcaggactt gcaggccgca tgcagcaagt gtgtaggcca
    10741 gggcggcata agcctgttgt gtcgtcccat accccgagca gagcagtacc agcgggcatt
    10801 gtgtgccgtc tactcgtctc ggaacatcgt gacacagtgc aatcgtgagg ccctcacgat
    10861 tgcgtaggtg gtgtgaaacg gtgaggaccg gcgagtctga catcggttcg gcaagatctt
    10921 gcgaagtgcc gatccgcggc gagataaggg cccggagtct ctgctcctgc gcgggcgata
    10981 aaccctcaaa gcgcacaccg aagcggcgtc ggactccttc tggtgctgcg ggattcgagg
    11041 ttgtcctgac ctgagattga tgtgtccaga caatccgcgc aggccaaggg gtttcagaac
    11101 cctcaggcaa ggcggcgccg attgacgaag tgtttggctc gacgggcacc aggtgaagga
    11161 cctgctgatt ttcagggaag tcggaacctc ccggtaatcc gatgcaggcc ccatccaggc
    11221 tcaggttgac cgtcactccg agaggacggg ttggcgtggc tgatgagccg accagtcgaa
    11281 tcgggcatgg gacgttgaga cgtgcggcct cacgtctatc tgtcggcgct gcggtggagg
    11341 ggaggggggc gtcggggttg ttcgcggcgg ttgtgaaaag aggaaggatt aatccttcga
    11401 acgtgacgat tgttgaaccg tcttgcagcc cgtcgagaag tgaacggagg acattgcggt
    11461 cgcgttcgcc gctcggtgaa aaggaaatca caaagttctt gatggcaggg agaggcaggt
    11521 ccgtaagtgc agccggtcgt tcatgcacga ctccttgaag ttccaggaag ctcaccggtg
    11581 tcttgatcac tacgtaggct ggttgcagat gaatggaggc cgacaggtca tggatggtga
    11641 ggagggcgcc gccggtgctc atgtttcgta agacgcctcg aagggtggtg cttccgacgg
    11701 atagttgcac ggggagcgcg aacagcttgc gttcggagcg ccgtcggtcg ggtcgttctc
    11761 cggcgacgtg ctgagattga actcgaacaa tgccgggttt ggcgggcgag gctgtgggag
    11821 attcagcgcc ctcatccgcg aagaaatgag tgggaacagg aattcgtacc gtgatggtca
    11881 gaccgctgtc gggtgcatgt tctatttcga tggcgccacc atgtgcctgg acaatttggt
    11941 gagcccgaaa gaactgctcg ggagaaatat ggaaatctgg ctgttcgccc atgggagcag
    12001 cggtgtggtt ggagctttcc ttgatggtga agctcacata ctcgcgcgag acgctcgaat
    12061 acagcggaag gttgcgtgaa gcgagtgaag gagtgactcc ggccagcagc gccgtctgta
    12121 gcagaagatg ggacggagcg ttgggcaagg acgtgtattg gtgtgcataa ttaaggagga
    12181 ttctcacgag atcctccagc gcgtccggtg agcccagaat gagtggaagg tgttgagcca
    12241 agtgcgtggt cacgaggcgc cccgagcctt ctgtctcgtg ccggatcgtt tgtaccacgg
    12301 tctcaagaac atggtgcaga tcgaggggga aaaactctaa aggaggggca tggatggctt
    12361 gagccagttg gtggccaagt tggctggcct gcgacgcttt ctcgatcatc gtttccacat
    12421 gctgctcgat cttgctgtgc tgaattgagc tgagcaatcg ttgtgaatcc tgcaatagcg
    12481 tggtgagagg ctgttggagc tggcgggata cccgggccgc ctcccgggaa agcgtgagtt
    12541 gacgggtggt ttcctggagg cgtgactcca actcggtgag tcggtcctgt tgcgccttct
    12601 gttgggaaag gtcgcgcagg accccgaccg tgccgagata acgattggcc gaatcgaaga
    12661 gtcccttggc tgttacatcg acggtgatcg atgcgggtgg tgcatctggt agggctttcc
    12721 gatgaagagt gagctcaagt ctgcgtaccg agcggctgcc ggcccgtcgt tcgttcaggc
    12781 gaaagcgtcc tgttgcctct tggaggggag ggagtagaag cgaatagtgt cgtccggcaa
    12841 gctcctcggg agtatagccg agcatggcgg tgacggcagt acccacgtag acaaagcgac
    12901 cgtcccgatc aagctcgtac accaagtcgc tcagcgtttc gatgaaccgc tggtgccgtt
    12961 gaaaggtgtg gtccagtttc atttggaggt cgcgcttttc aaccgcctcc tggacggaaa
    13021 acaacaactc ggtgataaag gcgggtgacc gtttgaacag gaggaagtca gcgccgtttt
    13081 gcagcgcctg gacggcggtt tgggaatcgc tttcattcgt ttggaggatg atggccgcat
    13141 agggagcatt gcgacgaagg cgggaaagaa tgtcgagacc gcgttcaggg cgtaaatcgt
    13201 gatcgatgag gatgatgtgc cattcgccct gatggctgaa tgtcagggct tcttcggatg
    13261 agtagcccgc ttcgatccgg cagccgggaa aaaatcctct caggctggag gtcaccagct
    13321 tgatttcatc cggttgttcg ttgatgagga gaatgttgag ggacggttca gccggcgaca
    13381 tggaagattt catggttcct atgcagtggt gcgatcgttc tgcttcggtg ccgccgggcg
    13441 ggcgcctggg aatgcaccac tgcacagtga tgaacatgcg ctcgctgtca gagggacaat
    13501 tgtgtcccca ggtagtcggt tggagtgcat gattggttca atatcataca ccttattgag
    13561 aatcccagcc cgctgcctga gggggcgcgt ttacttcagt aggggagggt gctgtgcgga
    13621 gcaagcaaaa gtgatcacac cccaagaata aggtgcaatt ctcgttgagg agggggttcg
    13681 gggcgagggt ttcgccggtc ggatgatgtg ccggcagcgt cgggaacgaa cgggatgccc
    13741 accgcaatcg ttggaccgcg gtgggcggag aactctccta tgcctagcga gctgaagctg
    13801 tgttggcaga ttgcgggcaa acgccagggg tcatatacag caggtaattt cccattccat
    13861 gctgaatatt cgggttttga agcgggtggt ggtgaaccat caccatttca tagtcgtcgc
    13921 gtttggttac cgtgaaccct ttgtcatccc ggtacacctg atgagggcga aaatcacgaa
    13981 acgtgccgtc ggcacccact tcagggaccg ttcgcaggag ggtttctcgg gaggatttat
    14041 tctccagggc aaccatcagc aattcatcgt ggccatgggg gtaggcgaat ttgacgcagc
    14101 cgtccatgtt gaatttcaaa ggggctgaca gaactgaaac cccgggcttg atttcgatgc
    14161 cttcgtcagt ttggtccgcg ccacgctggc cgaacttgct gtagcagaca atattgaccc
    14221 cgacctggta gacatccatt tcctgcacct tcgtgccttt cggcgcgaca tacatcgtga
    14281 aacgggccat cacgtctttt gtcgggggag ccttgtggta gaacgccaca acggacatga
    14341 ggttttgccc ttttcccagc ttgaccccgt atccgtccgg aaaacgtgct tccgtcattt
    14401 ccaaaccggc accggcgaag aacaacggct cgccgtcgca tgacacgctc ggcttgtcgt
    14461 tgttcagcat gagaatgtga tggaggtaat tcttgggcag cggcttgccg tctttcgtgt
    14521 agacctcgga cttatagccg atcatgtagg tgtcgtccgg caactggaag accttcttgg
    14581 gcatactgga tgccagatcg ccgtcatgac cggtcggcaa atcgaccgga ccgaaggtga
    14641 gagtgaccgt gtgatcatcg atggtaatct tagtgtcgcc taaactcggc atggttggaa
    14701 tggcatggtg atcgtggcca ccgtcggcgt agaccgtact tgtgaccatc agcagcatga
    14761 ccatcgatag cagcgcacga ttcatagccc ctcctcgtta taacagaccg gccgtccgtg
    14821 agtgtgtgtg aggcaatgtc gggaaatagg cccacccgtt agggagccgt cgggggcggg
    14881 gtcagtcgct tccacaggta agtaccgcca tgttgccggg gcggaatatt gtctctggtg
    14941 ccgacacgca cgtaccacca gattcccttg gcttcggtgc catcctccga gatcaccact
    15001 tcaaacgcgc cttcgcggtc attgccggtc tgtttccagg tcccctgcca gcgccggtcg
    15061 ttgaagctgg tggtggcgaa ctgtccccct tgccaggtgt aagggccgtt gccgtgcgcg
    15121 tccagagtgg cgacgtattt cttattgtct tcgatttcag tgacttccca ttcgccgctc
    15181 aagtccgcgc cattcggccc gggcgcctga ttgtctgaca cgcgacgcac taaggtgccg
    15241 gggtcggcca gggcgatccc cattccggta tcgggctgtc tagtggattc gtgccaggtc
    15301 acgagaatcc aggtgccgtc ccttcgctcc atcacccctg tctcacgcag tggaagcacc
    15361 atgcgttggg tcaggtccgt tgcgttgaga taacgaatat agtcgagttc catcgcgaac
    15421 caggcgatgt cacccttatg ccaaaattgc aggtaggtga tgggaagttc cagtttggcg
    15481 accgaggtga actcggtgcg catgtcggta gccaaagccg tccatcctac gtatttgtgt
    15541 ccgtcgatcg tgtagccgac ggcgtcggag tcgtgagaca tatacttcga catagtgggc
    15601 agatcacgat cggcgtttgc ttgcacgatg aggcgcagag tggcttctgc atcgactggc
    15661 tccttggccg ctgcaaggga gcccgccagg atgctcatga gggaacagat aaagaggcag
    15721 tgccgcaacg aggcgcggcg ggatcgacgt tcaatgggct gaaagtgcat aggggtatag
    15781 ggaaatatat ggacgaaagc ctcgtgattt tacgaggcgg caattctgcg aggctacctg
    15841 gtgcctcgag ttggtgtcaa tggatatttg cctgatcggg tatctcagta atggtgcaag
    15901 ccaccacggt gacgttgtcg atccctcccg catcgagcgc tgtgtgaatc agtgcctggg
    15961 tggcaaggga agggatatgc tgatgaggcc tcacggtctg cagaatgcgt tcgtcggtca
    16021 acatttttgt gaggccgtct gagcagagca gcaggaggtc tccatcgaga agatcgaaga
    16081 agaacaggtc gctttccagg tgaggggcaa ggcccatggc gcgggtgagc acatggcggt
    16141 tcgggtggca tgcggcctgc gcaggtgtga ggcgcccgtc acggacgtac tcttcaatca
    16201 gggtatggtc ccgtgtgagt tgcgtgaggg tgccggctct gaccaggtag gcccggctat
    16261 cgccgacgtt caaaacaagt aagcgtggct tgggcagcgg gaggagatgg gccatcacga
    16321 gtgtcgttcc catgccgtat agcggcgggt gctgagccgc atgggtgagg atggaggcat
    16381 gagcctgctc cgccatggtt gtgagacggt cggttgcgcg ctcttcctcg tgaaggtcgg
    16441 gggcagatgc aattccgccg agagtcggcg caagttcggc gagggagtcc attacgagac
    16501 ggctggccac atctcctccc gggtggcctc ccattccgtc cgccactgcc cagagtcgat
    16561 gtcggttctc aacgaggaag gtgtcttgat tcgtgggacg gaccaacccg cgctcggtct
    16621 gcccgaatgc cctccatgcc catctgttct gctgtgtgct cacgctatcc ggccggcgtc
    16681 aatgacggtg gaccgtagcg cagggcgagc cggtcatata gccactccgc cagtggttca
    16741 aggtttctgg tgtcggcggc attataacgg acgagttgat ccagcgctgc ctcgtcgcct
    16801 gcgcggtact ggtgccagag acgaacggct tccatgccgt cgagtgtcag cagatcggag
    16861 tcccgcgcga tgtccaactc gcgctcgatg tgcttgaggc ctccctgcag gcccacgcgt
    16921 ctcgcggcga aacagagatc gaagtgagga atcgacaccc gcaggccctt gaaacaggtt
    16981 tggaggtagg ggatatcgaa cacgctgccg aaaaacgtga tcagcaagtc ggcttggtcg
    17041 agttcttcct gaatcacgcc aagcgtcagg tcgtcaccgc tgatcagggt gcgcagtcgc
    17101 ccctggcggt agaggccgac gatggtcacc tgcccctcgt gcgcggacag tccggtcgtt
    17161 tcgatgtcca gatagagtgc ccgtgggccg aaggtgcgga acagccgcca gtggtcgcgg
    17221 gtatgcaggc agccggcaaa gtaccgggca ttgcggcggt cgagctgctc ctgcgccgag
    17281 gcaagatcga tatcgtacag gactttgcgc gagggtgaga tgccgggaag gttcggctca
    17341 cggcgaaagg tcgtccaatc ggcgatgccg ccttcccaga gccgccgctc cgtggacggg
    17401 ccgatgccgt tcagatggac gaatgtcgag gtcaacataa tgcgggtcgc gatctccctc
    17461 cgccgggatt gtagccttga tttcaaggga taaacaagtt agagtccgcg actggtgcgg
    17521 agtttcgtgc gcttgagttg tgcagtgtat cacggtgtaa atgagggaac atgaccacga
    17581 acggatcggc gaaacgtatt cgaatcaagg tcggcgggat acagttggag gcggagttga
    17641 aaggcacgag aacggctgaa gaactctacc gggcgctgcc cgctgaagga ccattgaatg
    17701 tctggggcga agagttctat tttaagattc ccggagtgaa ggaccatcgc gaaacggcga
    17761 ccacgcaggt caaagtcggt gacgtggcct tctggggcgc cggtcaggtg ttggcgattt
    17821 tcttcgggcg tacgccgatg agcatggggg ccgatcccgt gccggctgac cgggtcaatg
    17881 tggtgggtcg cgtcgtggga gacgcggcga ctctgcggca ggcgatgaat gccagcacga
    17941 ttcgagtcga acggattgaa caggggtcgt cgatcggata ggctcatggg gagtggtctt
    18001 gtaagcggcc atagtttgtc ggcggggagt cttctgcgat gaggctggca aaagagcggg
    18061 tccatcatat ggcggaatca gtgatcgcac ggctccacga gctgggtttt ctggacgtca
    18121 ccggggatcg aaaggcgctt cgggattcgt tggaacagac gatgacggaa gagctcggag
    18181 tggaagatcg cctcaatgct gaagtgcgcc ggatgatgca accctatgaa cggcagatcg
    18241 agcagggaca ggtcgattac cagaagatgt tcacgatgat caagcagaag ctggtgcgtg
    18301 aacggggcat cattttatag gtgctgaccg gtgtgtgaat cttcgtcata gaaaagcgca
    18361 gagtagcctc actcaggact ggagtccaat tcgtgatcag cgacgataaa gccagccatc
    18421 ttgcccacct cgctctcggc gccttgaaga agagcccgca ctgccggttt actgaagatg
    18481 atgggaaggt gctgcgcgag atcaaacggg tggtggccgc agagttgttg ttggaagcgg
    18541 agatcgataa gaaagtacgg accaaactgg cgtcctactc acgacaaatc gtggagggga
    18601 gtccggaatg ggacgtgatg tatcggaaaa cagctgaaga agaacaaaaa aagcgcgcca
    18661 agccctgatc cggcgtggcg agcgatttcg acgaggttgt gcgatgagag ttgctctatg
    18721 gatagtgttt tgcgtgatgg cggtgtcgtt gcccgcctgt acccattcca aaccgcagcc
    18781 gctggttccg ttggaattgg cctatggggc caaggctgac gccatcgctc tgaacaatca
    18841 ggggatgcaa gcctatctgg ccggacaggt tgcggaagcc aagaattttt tcgggcaggc
    18901 catcaaggcg tcgccggatt ccggccaggc ccattacaat tacgcgctcg cgctgaatgc
    18961 attggggcaa acggaccagg cccgcctgga gtttattgag gcggcgaatt tagctcccgg
    19021 ggataaggtg atctgggatt ctcctgcgtt acgccccttc ggcagtcctc aggcacaaaa
    19081 ggggccgccc agggaacacc cctatgggac acagcgtccg gtgatcggca gcggaccgcg
    19141 ctgaccggct gacacgaagc gttgtactgg agaggattga gacatggctg acgaattgga
    19201 cgtgggagcg aaggcgccgg atttttcctt gccggaccag gacggcagta ccgtgacgct
    19261 gaagagcctc aagggcaagc aggtggtcct ctatttttat cccaaggacg atacctcggg
    19321 ctgtacgaaa gaagcctgcg atttccggga ttcccttgcg ccgatcaaaa aggccggggc
    19381 ggtggtgatt ggagtcagta aggacgggaa agcctcgcac cagaagttta tcgccaagta
    19441 tggcttgccc tttgcgttgt tgagcgacga agaggccgag gtctgtaagg cctacggagt
    19501 ctataaagag aagagcatgt acggacggaa gtatctgggc atcgagcgga gcacgtttgt
    19561 gatcgatgcc acgggtcgga tcaaggccct tttccggaag gtgaaggtgc ccggccatgt
    19621 cgatgaagtg ctggcggcgc tgaaggccta gcagaatgcg gaaaaagtcc gccagcggcg
    19681 ttctcgcaag acactgccgc ctcaccggct cggcggcgtt cacaaacgtg acgcgc
//
LOCUS       DAOQRM010000031        19508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1934_length_19508_cov_17.521822, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000031 DAOQRM010000000
VERSION     DAOQRM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19508
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1934_length_19508_cov_17.521822"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..>1484
                     /locus_tag="PKK81_04470"
     CDS             22..>1484
                     /locus_tag="PKK81_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32153.1"
                     /translation="MTVQGSHIGIWDWNLTTGVIYFSPQWKNQLGYDDITLPNNFDEW
                     RTRIHPDDQPFVQETIQTCLDGQRSQFELEHRLRHRDGSYRWILSRGSLIRDVYGVTA
                     RMVGIHIDTTDRKQAEQELRAAKEAAEAASKAKSEFLANMSHEIRTPMNGVLGTTELL
                     LNSALTDKQRHLASTVHRSGRTLLAIINDILDFSKIEAGKLDLECVDFDLQPVLEESL
                     ELFGEAARRKQLRLSQHIDDAVPRYLKGDPVRFRQILMNLLSNAMKFTETGGVSLSAE
                     YVEGTTTQALLRFAVTDTGIGIPPAAKSRIFDAFSQADGSTTRRYGGTGLGLSIAKQL
                     VGLMGGTITVESTPGSGSTFAFTTRLDLQPLGSGLSTKLAPYMQWAEPLVSSGQTSDT
                     PCPHASEAGAAAPSPKQSGRILLAEDSPVNREVAVGMLEQLGYEVEVAENGRQALAAA
                     ERDRFDIVLMDCQMPEMDGLTATGEIRRRETASGRRRL"
     assembly_gap    1485..1584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1585..2243
                     /locus_tag="PKK81_04475"
     CDS             <1585..2243
                     /locus_tag="PKK81_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNV32154.1"
                     /translation="TATGEIRRRETASGRRRLPIIALTAHAMQGDREQCLTAGMDDYL
                     TKPFTQMELHGIVRKWLSHHEPASQAVQPNAAAPGTQSVPPRATTEPAADTLSASPGM
                     DLKALEGIRALQRPNRPNVLASVLRKYLDNSRESVDALRDAIRANDPMALEAMAHRLK
                     SSSAQLGAIAVAASCKELEVMGNRKQLVDADRIFAQLESDYRAACTVFRNEIAKENQT
                     "
     gene            2240..3487
                     /locus_tag="PKK81_04480"
     CDS             2240..3487
                     /locus_tag="PKK81_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32155.1"
                     /translation="MNPLIPGRAPYALISDDDIIIRMFAREALEQAGWVVEEAQNGIE
                     ACEIFQKCPPDVVLLDVMMPGMDGFATCAALRRLPGGEHTPILIMTGLDDFESITKAY
                     DAGATDFIVKPLNAMLLTHRIRYMVRAAQVLQELLESQATLTQARDAAIEGARLKSEF
                     LATMSHEIRTPMNGVLGMTDWLLDTPLTAEQLDCAQTIRSSGDALMVIINDILDFSKI
                     ESGKLSLELLDFDLPTFLDRVLALFGERAQRKGLLLTSRIAEDVPSGLCGDPARLQQI
                     LSNLLANAIKFSEHGSVSVLVELDQRPAEQGLEFPATRYGELSAQQERVTHIRFSVSD
                     NGIGIEPSAFSKLFQPFVQADGSTTRKYGGTGLGLAICKQLVELMGGHIGAESEPGSG
                     SVFRFTVPLQQQLPNSLSTRQAA"
     gene            3820..4713
                     /locus_tag="PKK81_04485"
     CDS             3820..4713
                     /locus_tag="PKK81_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNV32156.1"
                     /translation="MHCLTFDIEEHFQVSRFDSPIRRRHWGSFESRVTPNTCKVLDLL
                     ARYQTRATFFVLGWVAERHPGLIKQIAECGHEIASHGYGHELVTAQTPELFRADVRKA
                     KQILEDLTGSPVQGYRAPGFTITRETLWALPILAEEGYTYDSSIVPIRHDHCGLPGSD
                     PWHHLRQTSSGPIWEIPPSTVNLGGVRLPIAGGSYFRLLPFPALCGLLRRTEQKGRPL
                     VMYFHPWELDPLQPHMEGPLLSQFLHYLNLDKMEFRISTLMNTFRFGPIAEQIDLSPI
                     MPLDPTIFPALPPAEIAQDVA"
     gene            4872..5447
                     /locus_tag="PKK81_04490"
     CDS             4872..5447
                     /locus_tag="PKK81_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32157.1"
                     /translation="MHHGSRNRRQQSPNTTGLAWALACIILLPAAAAGQSLDDTVQFI
                     SDMANTHGFVRSLSCRNPKAGKPTKITEIYSVIPTGTKLGTIELNHGHDEVNTGFTHF
                     DLHDIDTIEYQGQDTKENNLVLYGVRITCKSSGPCIMKTSFCTGAVTELEAQFPEDTL
                     LFRSASHAERMTKALAHFLGLVRAQRQNQPF"
     gene            complement(5513..6163)
                     /locus_tag="PKK81_04495"
     CDS             complement(5513..6163)
                     /locus_tag="PKK81_04495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV32158.1"
                     /translation="MAQQRWSNRLRIAGMMSLFMTVGIAGIGLAADAAPAGSESVSAS
                     NARAVIKTKFGDIEIKFLPDVAPKHVENFIKLAKSGFYNGTIFHRAIPGFMIQGGDPN
                     TKDSLKKGAYGQGGPGHNVKAEFSDLPHKRGVVSMARAQDPDSAGSQFFVVVEESRFL
                     DRKYTIFGEVVKGIGVADKIVALPRVMCQTGAPSPADKGPCDNPIDRVEMTVTIIE"
     gene            complement(6201..6905)
                     /gene="rnc"
                     /locus_tag="PKK81_04500"
     CDS             complement(6201..6905)
                     /gene="rnc"
                     /locus_tag="PKK81_04500"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNV32159.1"
                     /translation="MTPATSIEAVQRLLGYRFHQPRLLEEALTHKSYSNERRSKDRTQ
                     NERLEFLGDAVLSLVMSEYLAAEFPGSNEGGLSKLKAHLVSEASLAKAARRMKLGRLL
                     RLGKGEELSKGREKHSLLADALEALIAAIYLDGGLEASRKFTLQVLEEELLATRAEQA
                     RPGMEDYKTQLQEVCQKRYESLPQYETVRESGPDHEKVFEVELTIQGVMRGIGRGRSK
                     KEAEQMAAKEALTQLA"
     gene            complement(6933..8183)
                     /gene="fabF"
                     /locus_tag="PKK81_04505"
     CDS             complement(6933..8183)
                     /gene="fabF"
                     /locus_tag="PKK81_04505"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNV32160.1"
                     /translation="MHDRPARRVVVTGLGLVTPLGTGVEKTWKALCAGESGIGRITRF
                     DPTGYDAQIAGEVKDFDPAQFIEKKEIKKMDTFIHYAVGASQLAVDDAKLKVAPEEAT
                     RVGVYIGSGIGGLGSIEHYHDVLREKGPGRVSPFFIPMTIINLASGQVAIRVGAKGPN
                     SCAVTACATGNHCIGDAYRLIQRDDADVMIAGGAEAAITPLGVAGFASAKALSFRNDD
                     PTKASRPFDKDRDGFVLGEGAGVVVLEELEHARARGARIYAEVIGYAMNSDAYHITAP
                     PEEGEGAVRCMEMALKDAGVAKTDIGYINAHGTSTMADAIETKAIKHVFGEQAYRIPV
                     SSTKSMTGHLLGAAGGIEAVFSILALHHGILPPTINLDHPDPACDLDYVPNTARSVQT
                     QVVLSNSFGFGGVNACLLFRRCAQ"
     gene            complement(8197..8430)
                     /gene="acpP"
                     /locus_tag="PKK81_04510"
     CDS             complement(8197..8430)
                     /gene="acpP"
                     /locus_tag="PKK81_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNV32161.1"
                     /translation="MATVDERVKKIIAEQLGVEEEEVTLEAHFVEDLGADSLDTVELV
                     MALEEEFEIEIPDEDAEKILTVGKALDYIKEKA"
     gene            complement(8530..9273)
                     /gene="fabG"
                     /locus_tag="PKK81_04515"
     CDS             complement(8530..9273)
                     /gene="fabG"
                     /locus_tag="PKK81_04515"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNV32162.1"
                     /translation="MSLQGKVAIVTGGAQGIGRAIAEALAEDGADIAVADLDPARSQE
                     AVAAIQKLGRRALSVKVNVADWNDAKAMADQVMQEWGKIDILVNNAGITRDGLLLRMK
                     EEDWNLVLQVNLNGTFHCTKAVLLPMTKQRYGRIVNIASIVGAMGNVGQANYAASKAA
                     VIGFTKTVAREYASRAVTVNAVAPGFIDTAMTQGLSPEVKEALQKQIPLGRLGLPSDI
                     AAAVRFLVSDAASYITGQVLHVNGGMLMV"
     gene            complement(9303..10256)
                     /gene="fabD"
                     /locus_tag="PKK81_04520"
     CDS             complement(9303..10256)
                     /gene="fabD"
                     /locus_tag="PKK81_04520"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNV32163.1"
                     /translation="MMASQIGFLFPGQGSQSVGMGRGFYEASPAVKAVYDEASSILGY
                     DVAQLCFEGPAERLNLTENTQPALLVSSAAALKALEPAGLKPVAVAGHSLGEYSAVYA
                     AGGVSFRDAVALVQKRGRYMSEAVPPGTGLVAALLGLSADVVKAVCQEAASVGVVAAA
                     NFNSPGQVVIAGEKAAVERAIEIAKTKGCKKAIPLPVSVPVHTPLMQKAADRLAGEFG
                     GVVWRDLTVPLVNNAEATALQRSEDIRASLVRQLPSSVRWEESVQTMAKLGVTTFVEI
                     GPGTVLTGLVKRILPGAVTANVNDPKSLEATLTMLGVNSQA"
     gene            complement(10363..11340)
                     /locus_tag="PKK81_04525"
     CDS             complement(10363..11340)
                     /locus_tag="PKK81_04525"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNV32164.1"
                     /translation="MIRARITGTGSYAPERVMTNADLEKLVATSDEWIRERTGIRERR
                     IAAEGQACSDLGLIAAERALKAAGISADELDMILLATCTGDMPLPSTACLLQHRLGAT
                     RAAACDVSAACCGFVYALGVADAYIRTGMRHILVVGSEVMSTITDWTDRNTCILFGDG
                     AGAAVVSASEGERGVLSTHLHSDGSLSDLIVVPGGGTRIPPSEAMVSERSQYIKMKGN
                     ETFKVAVRTLEEVARETLAAHHLSVDELDVYIPHQANLRIIKAVADRLNLPLEKVVLN
                     MDRYGNTSAASIPIALDEAVRDGRVKEGQLVMFGAFGAGLTWASALVRW"
     gene            complement(11337..12368)
                     /gene="plsX"
                     /locus_tag="PKK81_04530"
     CDS             complement(11337..12368)
                     /gene="plsX"
                     /locus_tag="PKK81_04530"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNV32165.1"
                     /translation="MKIAVDAMGGDHGPAPVIEGAMQAAQELGVGIILVGKQDELTAA
                     CQKLNCTDTRITIHHAPQVVEMHESPATVARKKRDSSIWVATELVKSGEADAVVSPGN
                     TGASMVASFFVLGLIKGVERPAIATMLPTLTGSAVMLDVGANVDCTAQHLEQFALMGN
                     EFAKHVYAKQNPRIGLLSIGEEDSKGNEVTKEAFKLLKASPLNFIGNIEGREVYSGNA
                     DVVVCDGFIGNVALKISEGVADVIKKLLMKEISGSWLGRLAYPLIAKPLLNLKRKIDY
                     AEFGGAPLLGVNGTTMICHGRSSAKAIKNAIRRAKGLAETRLDELIQRDIEESLRRHQ
                     DERPEGKQA"
     gene            complement(12520..12705)
                     /gene="rpmF"
                     /locus_tag="PKK81_04535"
     CDS             complement(12520..12705)
                     /gene="rpmF"
                     /locus_tag="PKK81_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNV32166.1"
                     /translation="MANPKHKHSRSRRDMRRTAKTRLVPPGFSLCPQCHELKLPHYTC
                     MNCGTYKGKAVIVVEES"
     gene            complement(12744..13415)
                     /locus_tag="PKK81_04540"
     CDS             complement(12744..13415)
                     /locus_tag="PKK81_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNV32167.1"
                     /translation="MSLGSSSRAVCLPLCSEAALPVMTVLMDTLKPAIVDIAPEGLSL
                     SCTATGPELGLDEPEEKCEGPLTIQLELVQHDGPITVTGTLEGTAIRQCVRCLTEYSD
                     PLFVNLYAEYLPQAGLATKPAPAEQGRRGPRRGAQPVEPVEEAEEADEVYLYQGEHLD
                     LVPMVREQVILAAPMQPLCREDCLGLCPQCGQNLNERRCGCPPEQGPSPFRVLRGRLS
                     KGGNA"
     gene            complement(13434..13691)
                     /gene="rpsR"
                     /locus_tag="PKK81_04545"
     CDS             complement(13434..13691)
                     /gene="rpsR"
                     /locus_tag="PKK81_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNV32168.1"
                     /translation="MERGDRGNGGGGGGRFFQRRKPCRFCVDKAPIDFKDVGLLRNFL
                     TERGRIVPRRISGNCLQHQRVLTVAVKRARQIALVSFAEER"
     gene            complement(13707..14099)
                     /locus_tag="PKK81_04550"
     CDS             complement(13707..14099)
                     /locus_tag="PKK81_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNV32169.1"
                     /translation="MAGFNKVILVGNLTRNPELRYTPSGTPVASFGLATSRRFKQGDE
                     LKEEVCFIDIVVFGKQAEHCGQYLSKGNGVIIDGRLQQRRWETEDGQKRSKHEVVAQG
                     VTFLPKRGEAGGESGGLHEEPSYEDEQL"
     gene            complement(14083..14436)
                     /gene="rpsF"
                     /locus_tag="PKK81_04555"
     CDS             complement(14083..14436)
                     /gene="rpsF"
                     /locus_tag="PKK81_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNV32170.1"
                     /translation="MELYESLFIIRPTLSDEETTALIEKMKGTVTKNGASLERAENWG
                     RKKLAYEIKRERKGTYVYFYFKGPGAVIAELERAYRLEDSIIKFLTVKLEQEPAPPRG
                     APVVAPQGATVGGVQ"
     gene            complement(14679..15044)
                     /locus_tag="PKK81_04560"
     CDS             complement(14679..15044)
                     /locus_tag="PKK81_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32171.1"
                     /translation="MTRLVPWFIVLCVILLLAGCAQSVHQTERLAQSDFLVDPGYAGS
                     QSYSYWPGPGYGPGYGIGYGPGYWGHPLGYYGGPYGPLGFGWYGGYGGFGAARPPSGT
                     PGPRPSLPPHAPPQFKKKL"
     gene            complement(15109..15411)
                     /locus_tag="PKK81_04565"
     CDS             complement(15109..15411)
                     /locus_tag="PKK81_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32172.1"
                     /translation="MTRSIGSLFVASALLLLTACAQSFHQGERLGSVGAHMNPGYPES
                     PSYWNGPGFWWNPFGYYGSGYGWYGGGYGIGSQQSNAPAPRPAPPANAPPQFQKKY"
     gene            complement(15423..16064)
                     /locus_tag="PKK81_04570"
     CDS             complement(15423..16064)
                     /locus_tag="PKK81_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNV32173.1"
                     /translation="MVWTRFVCSRLSAGLLGFVLLLCSPSPIRAEWYVAAQLGANFAD
                     PLRNVRGSGTLAGLDAPNFNLKTSPAFGGKLGVFPGHGLFGIELDVSHSTPHIKNLDD
                     VPGTHLSVTNIGAHVVLRYPGVTWQPYIGGGQTLLVARLGRSSTTESDTQVSVGGNVL
                     VGIRAFVTPKVALFTEYKYTDSTFRFGGAFGPVGGFDATYRAHQLFVGLSYHF"
     gene            complement(16227..16439)
                     /locus_tag="PKK81_04575"
     CDS             complement(16227..16439)
                     /locus_tag="PKK81_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32174.1"
                     /translation="MRKPGEPIYLWIHLLALLLVIIATVALPRAAEFVVGPLSFGTRA
                     LAGVGIAVAGGIALYLLYNSSARNEP"
     gene            complement(16529..17734)
                     /locus_tag="PKK81_04580"
     CDS             complement(16529..17734)
                     /locus_tag="PKK81_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32175.1"
                     /translation="MTTSSHQSLSLAQFDLSPDRGFLPATDPLAQLPDEPILNQIGAE
                     LPKFLTARQFRSFVQDHQEVMGPVADDWGLDVFRCAMRTLSFAGHAFVWEDPDHPASL
                     IPASLALPWCAVAQQLGRPPVLSYASYALHNWRRLDPQKPIELGNVVLLQNFLGGLDE
                     EWFVLIHIDIEAKAGPGLVGLVQAHNAARDNMPDDVLQGLHALAAAQEAMRETLLRMP
                     ERCDPYIYYSRVRPYIHGWKNSPSLPQGVVYEGVADYAQRPQQFRGETGSQSSIVPAL
                     DAGLGIPHADDPLTQYLVEMRDYMPPRHRTFLAALEARTDEQHRPLLYGYARDRKATH
                     PDLWKAFCTCVDLLAQFRDIHIGYAERYIFRQHQSHASNPTAVGTGGTPFMPYLSKHL
                     EETKRFTLD"
     gene            complement(17862..18452)
                     /gene="pth"
                     /locus_tag="PKK81_04585"
     CDS             complement(17862..18452)
                     /gene="pth"
                     /locus_tag="PKK81_04585"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNV32176.1"
                     /translation="MRLLVGLGNPGADYADTRHNIGCWVIERAAARWSIRLTRKGAAQ
                     RGSGRAGSKLVELASTLDWMNLSGPPLKGLLRELGLTPEALVVVHDDLDLEPGRVRIR
                     QDGGSGGHNGIKSVIEALGTPQFVRLKIGVGRPAPRQDTADYVLEQVSPEERAIYVPC
                     LERAVDALELLLNRDVATAMNQFNVREKAEGGERPS"
     gene            complement(18458..19225)
                     /locus_tag="PKK81_04590"
     CDS             complement(18458..19225)
                     /locus_tag="PKK81_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNV32177.1"
                     /translation="MKFDLTVESREGGGKGPARQLRRAGRVPAVLYGQGECLLLSVKP
                     DELVKILRSQAGSTALISLAINGAKSKAKRTALLREFQVDPITGSVLHADFFEVAMDK
                     PIRVKVPVHLTGGQPLGLKEGGVLHHVLRQLHIECLPSVLPDFIEVDASQLQINQGIH
                     LKDIPKTEGLRFLDDLEHMVVSVAAPMTDAKLEALLTAGAPGAEGAKEPEVAAKGKAV
                     AEGAAGAEAGKAGDAKAGAAAPKAGAAPAKKEAEKKK"
     gene            complement(19263..>19508)
                     /locus_tag="PKK81_04595"
     CDS             complement(19263..>19508)
                     /locus_tag="PKK81_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HNV32178.1"
                     /translation="QACLDKGAREVWAGCSHGVLSGPALERLQQSGLTEVLVTDSIPL
                     RGKEQKCPKLKVLSVAPLFGEAIRRIHEDESVSSLFV"
BASE COUNT         4093 a         6263 c         5196 g         3856 t
ORIGIN      
        1 acgagcgaag cacggttgac gatgaccgtg cagggctcgc acatcgggat ctgggactgg
       61 aacctcacca caggcgtgat ctatttctct ccgcaatgga agaaccagct cggctacgac
      121 gacatcacgc tccccaataa cttcgacgag tggcggacgc ggatccatcc tgacgaccaa
      181 ccattcgtgc aggaaaccat ccagacctgt ctggatggcc aacgatccca gttcgaactt
      241 gaacatcgtc ttcgacatcg agacggctct tatcgatgga tcctgtcccg cggcagtctg
      301 attcgagatg tttatggcgt aaccgcgcgc atggtcggca tccacatcga taccaccgac
      361 cgtaaacagg ctgagcagga gctccgtgcc gcgaaggaag ccgcagaggc cgccagcaaa
      421 gcgaagagcg agtttctggc caatatgagt cacgaaatcc gcactcccat gaacggagta
      481 ttgggcacaa ccgaactgct gctcaacagt gcgctgacgg acaaacagcg acatctcgcg
      541 tcgaccgtgc atcgttccgg aagaacgctg ctggccatca tcaacgacat tctggacttt
      601 tcaaaaatcg aagccgggaa actcgatttg gaatgcgtcg acttcgacct gcaaccggtg
      661 ctcgaagaat cgctcgagct gttcggcgaa gcggcccgtc ggaaacagct tcgcctttct
      721 caacacatcg acgacgcggt cccgcgctac ctcaagggcg acccggtccg gttccgccag
      781 atcctcatga atctgctgag caacgcgatg aaatttaccg aaacaggggg cgtctcgttg
      841 agcgccgagt atgtggaagg aaccaccacc caggccctgc tgcgcttcgc cgtgaccgac
      901 accggcatcg gcattccgcc ggcggccaag tcacgcatct tcgacgcatt ctctcaggcg
      961 gacgggtcga ccaccagacg atacggcggg accggtctcg gcctttccat tgccaagcag
     1021 ttggtcggac tcatgggtgg gaccatcacc gtggaaagca caccgggaag cggctccacc
     1081 ttcgcgttta cgacgcggtt ggatctgcaa cccctggggt cgggcctctc cacgaagctc
     1141 gcgccgtata tgcaatgggc agagcccctt gtctcgtccg gacagacgag cgacacccca
     1201 tgcccacacg catctgaggc gggagcagca gcgccgagcc ccaaacagag cgggcgaatt
     1261 ctgttggccg aagacagtcc tgtgaatcgc gaagttgccg tcggcatgtt ggagcaactg
     1321 ggctatgaag tcgaggtggc ggaaaacggc cggcaggccc tcgcggccgc cgagcgcgac
     1381 cgtttcgaca tcgtgctcat ggactgccag atgcctgaaa tggacgggct cacggccacc
     1441 ggagaaattc gccgtcgcga aacagcctcc ggtcggcgtc ggctnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnntcacgg ccaccggaga aattcgccgt cgcgaaacag
     1621 cctccggtcg gcgtcggctc cccatcatag ccttgacggc gcatgccatg cagggggacc
     1681 gtgaacaatg cctcaccgcc ggcatggacg attatctgac taaaccattc acccagatgg
     1741 agttgcatgg aatcgtccgc aaatggctga gtcaccatga gcccgcctcg caggccgtac
     1801 agccgaacgc tgcagctccc ggaactcaat ccgtgccacc ccgagccacg actgagccgg
     1861 cagcggacac cctatctgca tcgcccggca tggacctcaa ggccctggag ggcatccgtg
     1921 cgttgcaacg tcccaaccgg ccgaacgtgc tggcctccgt gcttcgcaag tatctcgaca
     1981 attcgcgaga aagcgtggat gcactacgtg acgccatccg cgccaacgac ccgatggccc
     2041 tggaagcaat ggcgcatcgc ctcaaatcaa gcagcgcgca actcggcgcc attgccgtag
     2101 ccgccagctg caaagaactc gaggtcatgg ggaaccgaaa acagctcgtc gatgccgacc
     2161 gaatctttgc ccagttggaa tccgactatc gtgccgcttg taccgtcttt cgaaatgaga
     2221 tcgccaagga gaaccagaca tgaacccgct gataccagga cgagcaccct atgcactgat
     2281 ttcggacgac gatatcatca tccgtatgtt cgctcgggag gcactggagc aagccgggtg
     2341 ggtggtcgag gaggctcaaa acggaattga agcctgcgaa atttttcaga agtgtccgcc
     2401 ggacgtggtg ctgctggacg tcatgatgcc cgggatggac gggttcgcca cctgcgcggc
     2461 attacgccgg ctgccgggtg gcgaacatac ccccattctc atcatgaccg gactggacga
     2521 ctttgagtcg attacgaaag cctacgatgc cggcgcgacc gattttatcg tcaaaccact
     2581 gaatgcgatg ctgttgaccc acaggattcg ctatatggtg cgcgccgccc aagtcctcca
     2641 ggaactcctg gagagtcagg ccaccctcac gcaagctcgc gatgccgcaa ttgaaggcgc
     2701 ccgactcaaa tccgaatttc tcgccaccat gagtcacgaa attcgcaccc cgatgaacgg
     2761 cgtcctgggc atgacagatt ggttgctgga cacacccctt accgccgaac agcttgactg
     2821 cgcccagacg atccgctcat cgggcgatgc cttgatggtc atcatcaacg acattctgga
     2881 tttctccaaa atcgaatccg ggaaactgtc ccttgaactg ctggacttcg acttgcccac
     2941 atttctagac cgtgtcctgg cactctttgg agaacgagca caacggaagg gtttgttgct
     3001 gacttcgcgg atcgccgagg acgtgccaag cggcctgtgt ggtgacccgg cgagactgca
     3061 acagatcctc agcaatttgc tcgcgaacgc catcaagttc agtgagcacg gatccgtctc
     3121 cgtcctggtg gaactcgatc agcgaccagc cgaacaaggc cttgagtttc ctgcgaccag
     3181 gtatggcgaa ctgtcggcgc agcaggagcg ggtgacgcat atccgatttt ccgtctcgga
     3241 caatggaatc ggaattgaac ccagcgcctt ttcaaaatta ttccagccgt ttgtgcaggc
     3301 tgacgggtcc accaccagga aatatggcgg aactgggttg ggactggcca tctgcaaaca
     3361 gcttgttgaa ttgatgggtg ggcatatcgg agcggaaagc gaaccagggt ccggttccgt
     3421 ctttcgattc accgtgccgc ttcaacaaca attaccgaac agcctctcca ctagacaggc
     3481 ggcctgagca ccaagcctca aggctcccat agcacctcat ttcccctcag cctatcagcg
     3541 caccaatcat cacctcatca ccacgcatga gtgtatctat taagatactc gatgcgtgaa
     3601 ttcgatgata cggtcgcgcg aagtatgccc gcctataatt gctatcagat tgaaatgctt
     3661 agctaaagtg catggcttaa actttgcttt acttcgtcct gcgcacatag tatacaaagc
     3721 ggtcgaaaag atcttcttgg tagccttctt cagcatgcat atggcagctc ttacttttct
     3781 cgcttagtag gcaaaaccct acacccaagg atcacaccag tgcattgcct gacattcgac
     3841 atcgaagaac actttcaagt ttcccgtttc gattccccaa ttcgtcgacg gcattgggga
     3901 tcatttgaaa gtcgagtcac tccaaacacc tgcaaggtgc tggatctcct cgcccgctac
     3961 cagaccaggg caaccttctt cgtcttggga tgggttgcgg agcgtcatcc cgggctcatt
     4021 aagcagatcg ccgaatgcgg ccatgaaatt gcctcacatg ggtacggcca tgagctggtc
     4081 acagcccaga cgcctgaatt gtttcgtgca gacgtgagaa aagccaaaca gatcctggaa
     4141 gaccttacgg gctctccggt ccagggctat cgcgcgccgg gcttcaccat tacgcgtgaa
     4201 acgctgtggg ccttgccgat tctggcggag gagggttaca cctacgactc cagcatcgtc
     4261 ccgatccggc acgaccattg cggcctgccc gggtccgatc cctggcacca tctgagacag
     4321 acctcttccg gccccatttg ggaaatcccc ccatccacgg tgaacctcgg cggagtgcga
     4381 cttcccattg ccggtggcag ctacttccgg ctgctcccct tcccggcgct ctgcgggctg
     4441 ttgcgacgga ctgaacaaaa aggtcgtccc ctggtcatgt atttccatcc gtgggaactc
     4501 gatcctctac agccgcacat ggaaggaccg ctgctctcgc aattcctcca ctatctcaac
     4561 ctcgacaaga tggagttccg gatttctaca ttaatgaata cgttccgatt cggccccatc
     4621 gccgaacaga tcgacctgtc gccgatcatg cctctcgatc cgacgatctt cccggctctt
     4681 cctcctgccg aaatcgcgca agacgtggcg tagtcacgtc gatacttccc acgcaggcat
     4741 tgcccgaccc accggcagca gtgcaccatt gctcacgctg cgccaaagta gggattgcag
     4801 tgtgtatgcg gtttcagtac ggtaggggtc gcctgaacga atccagcaca acgccctcac
     4861 ggagactgac catgcatcac gggagtagga accggcgaca gcagtcgccc aacacaacgg
     4921 gcctggcatg ggcactagcc tgcattattt tattacccgc agcggccgcc ggacagagcc
     4981 tggatgacac cgtgcagttc atcagcgaca tggccaatac tcacggcttt gtcaggagcc
     5041 tgagttgcag aaatccgaag gcgggaaaac cgacgaaaat taccgagatc tacagtgtca
     5101 ttccaaccgg aaccaaactc ggcacgatcg aactcaatca cggacatgat gaggtcaata
     5161 cgggcttcac gcacttcgat ctgcacgata tcgacacgat cgagtatcag ggccaagaca
     5221 cgaaagagaa caacctggtc ctgtatggcg tccgcatcac gtgcaagagc agcggcccct
     5281 gcatcatgaa gaccagtttc tgtaccggag ccgtcaccga actcgaagca cagttccctg
     5341 aagatacctt gctgtttcgc tccgcctctc atgccgagcg aatgaccaag gcgctcgctc
     5401 acttcttagg gttggtccgc gcccaacggc agaaccaacc cttctgaccg atatcggtct
     5461 gctccgggcc gacactcgtg aagagatcgg cccggacaca ccgctgtctc acctactcaa
     5521 taatcgtcac agtcatttca acccgatcga tcgggttgtc gcatggcccc ttatccgcag
     5581 gactcggtgc gccggtctgg cacatgactc gcggcagcgc cacgatcttg tccgccaccc
     5641 cgatgccttt gacgacctcg ccgaagatcg tatatttacg atctaagaac cgggactcct
     5701 cgacgacgac aaaaaactgg gatcctgcgc tgtccggatc ctgcgcccgc gccatggata
     5761 ccacgccacg cttgtgaggg agatcgctaa actcggcttt gacattgtgc cccgggccgc
     5821 cctggccata cgcccccttc ttcagcgaat cctttgtgtt gggatcgcct ccctggatca
     5881 tgaatcccgg gatcgcccgg tggaaaatcg taccgttata gaaccccgac ttggccaact
     5941 tgatgaaatt ctcgacatgc ttcggggcga cgtccggaag aaacttgatc tcgatatcgc
     6001 cgaacttcgt cttgatgacc gcacgcgcgt tactcgcact gacagattcg cttccggccg
     6061 gagcggcatc ggcggccagg ccgattcccg cgatcccgac tgtcatgaac agactcatca
     6121 ttcccgcgat tcttaaacga ttgctccatc gctgctgcgc catcaccatg cctccttcta
     6181 gtcggtccgc gacctcttgc tcacgccaac tgcgtcaacg cttccttcgc cgccatttgc
     6241 tctgcttctt tcttactgcg accacgcccg atccctcgca tgactccctg aatggtcagc
     6301 tcgacctcaa agactttctc atgatcagga cccgattccc gaaccgtctc atactgcggc
     6361 aaactttcat accgtttctg acagacctcc tgcagctgcg ttttgtaatc ctccattccc
     6421 ggccgggcct gctcagcgcg cgtcgccagc aattcctcct ccagcacctg caaggtgaat
     6481 tttctgctgg cctcaagccc accgtccagg tagatggcgg caatcagcgc ttcgagcgcg
     6541 tcggctaaca gcgaatgctt ctcgcgccct ttcgaaagtt cttcgccttt ccccagacgc
     6601 aacagccgac ccagcttcat gcgcctggcc gccttcgcga gcgaagcctc gctgaccaga
     6661 tgcgctttga gcttggaaag cccgccctcg ttgctacccg gaaactcggc cgcgagatac
     6721 tcgctcatga caagcgagag caccgcgtcg ccgagaaact ccaggcgttc gttctgggtt
     6781 cgatctttgc tgcgccgttc gtttgagtag gacttgtgag tcagagcttc ttccaacaga
     6841 cgaggctgat ggaagcggta gccgagaagg cgctggaccg cctcaattga cgttgccggc
     6901 gtcatacgac ctgactcgcg ccctgaagtg gattactgag cacaccggcg aaagagcagg
     6961 caggcgttca ccccgccaaa accgaaggag tttgaaagga ccacctgcgt ctggacggaa
     7021 cgcgctgtat tgggcacgta gtcaagatca caggcgggat cgggatgatc aaggttgatc
     7081 gtgggaggca aaatgccatg gtgcagcgcc aaaatgctaa acaccgcctc aataccgccg
     7141 gccgcaccca agaggtgtcc cgtcatggat ttcgtcgaac tgacaggaat ccggtaggct
     7201 tgctccccaa acacatgttt gatggccttg gtctcgatgg catccgccat ggtagatgtg
     7261 ccgtgggcgt tgatataacc gatgtccgtc ttggccacac ccgcatcctt caatgccatt
     7321 tccatacaac ggaccgcccc ctccccttct tcaggcgggg cggtaatgtg gtacgcgtcg
     7381 ctgttcatgg cgtacccgat cacttcggca tagatgcggg cgccacgcgc gcgggcatgc
     7441 tcaagttctt caagcaccac cacgccggcc ccttcaccca atacgaaccc atctcgatcc
     7501 ttgtcgaacg gacggctggc cttcgtcggg tcgtcatttc tgaaagagag ggctttcgcc
     7561 gaggcaaacc ctgccacgcc gagcggcgta atcgccgcct cagcaccgcc ggcaatcatc
     7621 acatccgcat cgtcacgctg aatgagtcga taggcatcgc cgatacagtg attaccggtg
     7681 gcacaggccg tcacggcgca ggagttcggc cctttggcgc cgacccgaat cgccacctgc
     7741 ccggaggcga ggttgatgat cgtcatcggg atgaaaaacg gagagacccg cccggggccc
     7801 ttttccctga gtacgtcgtg gtaatgttcg atcgacccca gcccgccgat accggacccg
     7861 atgtagaccc cgaccctcgt ggcttcttca ggcgccacct tgagtttcgc atcgtccacc
     7921 gccagctgac tcgcccccac cgcatagtgg atgaaggtgt ccatcttctt gatttctttt
     7981 ttctcgatga actgagccgg gtcgaaatcc ttcacctctc cggcaatctg cgcatcatag
     8041 ccggtcggat cgaatcgcgt gattcggccg atccccgact ctccggcgca cagcgctttc
     8101 caggtcttct ccaccccggt tccaagcgga gttaccagtc cgagaccggt caccaccaca
     8161 cgtctggcag gtcgatcgtg catacctacg gttccgttat gccttctctt taatataatc
     8221 cagcgctttg ccgacggtga ggatcttctc ggcatcctca tcggggatct cgatctcgaa
     8281 ctcttcttcg agagccatca ccaattcgac cgtgtcgagc gaatcggcgc ccaaatcctc
     8341 gacgaaatgg gcctcgagcg tcacttcttc ttcctcgacc cccaactgct cggcaataat
     8401 tttcttcacc cgttcatcta ctgttgccat ggacttgccc acctccttcc ccatcttact
     8461 tgacctccac attcctcacc gggagattcc ggatcgagga caccaacccg gacacccgac
     8521 ctgactcact tataccatga gcatgccacc gttcacgtgc aacacttgcc ccgtgatata
     8581 cgacgctgca tccgacacca gaaaccgaac cgccgccgca atgtcggagg gaagccccag
     8641 gcgtcccagc ggaatctgct tctgcaacgc ttccttgacc tccggcgaca gcccctgggt
     8701 catcgccgta tcgataaagc ccggcgccac cgcattcacc gtcaccgcac ggctcgcata
     8761 ctcacgcgcg accgtcttgg taaacccgat caccgccgct ttggacgccg cataattcgc
     8821 ctgtcccaca ttccccatcg ccccgacgat cgaggcaatg ttgacgatcc gcccgtaccg
     8881 ttgtttcgtc atcgggagca ggaccgcttt cgtgcaatga aaggtcccgt tcaaattgac
     8941 ctgcagaacc aggttccaat cttcttcttt cattctaagc aacaatccat cacgggtaat
     9001 accggcgttg ttgactagaa tatcaatctt cccccactcc tgcatcacct gatcggccat
     9061 ggccttggca tcattccaat cggccacgtt caccttcaca ctgagcgccc gtcgccctag
     9121 cttttggatc gccgctactg cctcctgcga acgcgccgga tcgaggtcgg caaccgcaat
     9181 gtctgcccca tcttcggcca acgcttctgc aatggcccgt ccgatcccct gggctccacc
     9241 ggtgacaatc gccacttttc cctgtaatga catcagcctg atccccgcaa tctcaactcg
     9301 tcttacgcct gactgttcac gcccagcatt gtgagcgtcg cctccagcga cttcggatca
     9361 ttgacgttgg ccgtgacagc ccccggaaga atcctcttca ccaaccccgt cagcacggtt
     9421 cccggcccga tctcgacgaa ggtcgtcacg ccgagcttgg ccatcgtctg caccgactct
     9481 tcccaccgca ccgatgacgg caactgccga accagtgagg cccggatgtc ttcggatcgt
     9541 tgcagggccg ttgcttcagc attattcacc aacggcacgg tcaaatcacg ccagaccact
     9601 ccaccgaact cccctgcgag tcggtctgct gccttctgca tgagcggcgt atggaccggg
     9661 acactgaccg ggagtggaat cgccttctta catcccttgg ttttggcaat ctcaatggcc
     9721 cgttcgacgg cggccttttc tccggcgatg acgacttggc cgggagaatt aaaattcgcg
     9781 gccgccacca caccgaccga agcggcctcc tgacagaccg ccttgaccac gtccgcagaa
     9841 agccccagta gcgcggcgac gagtccggtt ccaggcggaa ccgcttctga catatagcgt
     9901 cctcgcttct gcacgagggc caccgcatct cgaaacgaca ccccgccggc cgcatagacc
     9961 gccgaatatt caccgagact atgccctgcc acagccaccg gcttcaaccc ggccggctcc
    10021 aatgccttga gcgccgccgc gctactcacc agcaacgccg gctgggtatt ctccgtcaaa
    10081 ttcaaccgtt cagccggacc ttcaaaacag agctgggcga cgtcgtatcc aagaatcgag
    10141 gaggcctcat cgtagaccgc cttgaccgcc ggagacgcct cgtaaaaccc gcgccccatt
    10201 cctacagact gcgacccctg tccggggaac aaaaacccaa tttgtgacgc catcatagcc
    10261 gcgtaaggta tcgtagaaac cacgctcaat gtcaacggga aaaagctgca cgaaccccgc
    10321 gcgtcagtcg cgaccgaccg gccacgacca gacgctcccc gactaccagc ggaccaatgc
    10381 agaggcccag gtcaagcccg ccccgaaggc gccgaacatg accaactgcc cttctttgac
    10441 ccgtccatcc cgcaccgctt catccaaggc gatgggaatc gacgcagcgg aggtgttgcc
    10501 gtagcgatcc atattcaaca ccactttttc cagcggcaga ttcaggcggt cggcaaccgc
    10561 tttaatgatc cgtaagtttg cctgatgggg gatatagaca tcgagttcat cgaccgacag
    10621 gtgatgcgcc gcaagtgtct cccgcgccac ttcctcaaga gtccgcaccg ccaccttgaa
    10681 cgtttcattg cccttcatct taatgtactg cgagcgctcg ctcaccatgg cctctgacgg
    10741 gggaatgcgc gttccaccac cgggcaccac gatcaaatca gagaggcttc catccgaatg
    10801 caagtgagtg gagagcaccc cgcgctcacc ttccgatgca ctcacaaccg ccgcaccagc
    10861 gccgtccccg aacagaatac aggtgttgcg atccgtccaa tccgtgatcg tcgacatcac
    10921 ttccgaccct acgaccagaa tatgccgcat accggttcga atataggcat cggccacacc
    10981 aagcgcgtag acgaatccgc agcaggcggc agacacatca caagccgctg cgcgggtcgc
    11041 tcctagccgg tgctggagca ggcaggcagt ggagggtaac ggcatatccc ccgtgcaggt
    11101 ggccaggagg atcatgtcga gttcatcggc ggaaatgcct gcggctttca aggcgcgttc
    11161 cgcggcaatc aggcccaagt cggaacaggc ctgcccctcg gcggcaatgc gacgttcgcg
    11221 aatgcccgtg cgctcgcgaa tccactcatc ggacgtcgcg accagcttct ccaaatcggc
    11281 attggtcatc acccgttcag gagcatacga cccggttccg gtgattcgcg ctcgtatcac
    11341 gcctgcttcc cttccggtcg ttcgtcctgg tgccgtctca ggctttcttc aatgtcacgc
    11401 tggatcaatt cgtccagcct ggtctcggct aagcccttcg cgcggcgaat cgcattcttg
    11461 atcgctttgg cggatgaacg gccatggcag atcatagtgg tcccattcac tccgagcaga
    11521 ggcgctcccc cgaactcggc ataatcgatc ttccgtttca gattcagcaa cggcttcgca
    11581 atcagcggat aggctaatcg cccgagccag gagccggaga tctccttcat caacaacttc
    11641 ttgatgacgt ccgcaacccc ttcggagatc ttgagcgcca cattaccgat gaatccatcg
    11701 cagaccacca catcggcatt gccgctgtac acttcccgtc cctcgatgtt gccgatgaaa
    11761 ttcagcggac tggccttcag cagtttaaag gcttctttgg tgacctcgtt gcccttgctg
    11821 tcttcctcgc cgatgctcaa gagccctatg cgcggattct gcttggcgta cacgtgcttg
    11881 gcgaactcgt tccccatcag ggcgaattgc tcaagatgct gtgccgtgca gtcgacattc
    11941 gcgccgacat cgagcatcac cgcgctgccc gtcaaggtgg gcagcatggt ggcgatcgcc
    12001 gggcgctcca cacctttgat caatccgagc acaaaaaacg aggcaaccat gctcgcgccg
    12061 gtattccccg gactcaccac cgcatcggct tcaccggatt tcaccaactc cgtcgccacc
    12121 cagatggacg agtcacgctt cttccgtgcc acggtcgccg gcgactcgtg catctccacc
    12181 acctgcgggg catggtggat cgtgatccgc gtatcggtgc aattaagttt ttgacatgcg
    12241 gcggtcagct catcctgctt gccgaccaga atgatcccga cacccagttc ttgcgcggct
    12301 tgcatcgcgc cttcaatgac cggggccggt ccgtggtctc cgcccatcgc atcgacggca
    12361 atcttcatgt gcggttagcg gtccacggat agagatgtga agaatcgtgt agagacgcct
    12421 tgccggctag gacctgaacg gcctcacgca tgacatagcg tcggatcgtg atcacggtgc
    12481 gcaattgtac ccgaatcacg tctggaaaat aaaactcggc tagctttcct cgacgacgat
    12541 gacggccttc cctttgtagg tcccgcaatt catgcacgta tagtgcggca gctttaattc
    12601 gtggcattgc gggcacagcg aaaacccagg gggaaccagc ctggtcttgg ccgttcgccg
    12661 catatctcgc cgggaccgtg aatgtttgtg ttttggattt gccatgacga ctccttacaa
    12721 cgggtacatg cccggttaac agactatgcg tttccaccct tggataggcg cccccgcagc
    12781 acacgaaagg gactcggccc ttgctccggc gggcagccac agcgacgctc attgagattc
    12841 tggccgcact gcggacagag accaaggcaa tcttcccgac ataacggctg catcggagca
    12901 gccaggatca cctgctcgcg caccattggc accaggtcga ggtgttcgcc ctgatacaga
    12961 tagacttcgt ccgcctcttc cgcctcttca acaggctcaa ccggctgggc accgcgtctc
    13021 ggccctctcc ggccctgttc cgccggagcc ggcttggtcg ccaggcccgc ctgcggaaga
    13081 tactcagcat agagattcac aaagagcgga tcggaatatt ccgtgaggca gcggacacac
    13141 tgacggatcg ccgtcccttc caacgtgccg gtcaccgtga tcggaccgtc gtgttggaca
    13201 agttccagtt gaatcgtaag cggcccctca cacttctctt ccggctcatc caagccgagt
    13261 tcaggccccg tggccgtgca cgacagagac agtccttctg gggcgatatc gacaatcgct
    13321 ggcttgagcg tatccatcag caccgtcatg acgggaagag ccgcctccga acagagcggg
    13381 agacagactg cccggctact tgatccaaga ctcaccaaaa cctatccctc gccctatcgc
    13441 tcttcagcga aactaaccaa ggcgatctgg cgcgcccgct ttaccgccac cgtcaacacg
    13501 cgctggtgct gcaggcaatt tccggaaatg cggcgtggga cgattcgtcc gcgctcagtc
    13561 agaaaattcc gcaacaggcc gacatccttg aagtcaatcg gcgccttatc gacacaaaac
    13621 cggcacggct tgcggcgctg aaaaaaccga ccgcctccgc ccccgccatt cccgcgatct
    13681 cctcgttcca tactcgcctc cgtctgctac agctgttcgt cttcgtaaga aggttcttcg
    13741 tgtaggccgc cgctctcgcc tcctgcctcg ccccgcttcg gtaggaaggt cacgccttgg
    13801 gccacgactt catgtttgct gcgtttctgt ccgtcttcgg tctcccagcg tcgctgctgg
    13861 agccggccat cgataatgac gccgttgccc ttgctgagat actgcccgca atgttccgcc
    13921 tgtttcccga acaccacgat gtcgatgaaa cagacttctt ccttgagttc atccccctgc
    13981 ttaaaacgcc ggcttgtcgc caacccgaag ctggcaaccg gcgttccgct gggcgtataa
    14041 cgcaactcgg gattgcgggt caggttcccg accagaatga ctttattgaa ccccgccaac
    14101 ggtggctcct tgtggtgcga ccacgggggc acctctgggc ggagcaggct cctgctccaa
    14161 cttcacggtc agaaatttaa tgatcgaatc ttcaagccgg taggcgcgtt cgagctcagc
    14221 gatgaccgct ccgggcccct tgaagtagaa gtagacatag gtgcccttgc gctcgcgctt
    14281 gatctcgtag gcgagtttct tgcggcccca attctcggcc cgctcgagcg aggctccgtt
    14341 cttggtcacg gtgcccttca ttttttcaat caacgcagtg gtttcttcgt cgcttaaggt
    14401 cggacgaatg ataaacaggg actcgtagag ttccatgcag aatcctcctg ggcatcggcc
    14461 cccattcaca gatgggagcg aggtgtaggt acgccggaca agccgggacg agctggcgaa
    14521 ggtagcacag cttcaaagaa tgtgtcaaac aggtgggcgg cttttcccgc gatgccgaga
    14581 ccggcacgcc ccaatgcgag ggcccacatc ctgtggcctg ccgtcgcagg aagcaacagc
    14641 acaggaacca tgcggtggct tcgcgatgac acccaggctc acaatttttt cttgaactgc
    14701 gggggtgcgt gaggcgggag tgacggacga gggccaggcg tgccggatgg aggacgcgct
    14761 gcgccgaagc cgccataccc accataccag ccgaacccga gcggcccgta gggaccgcca
    14821 taatacccga gcggatgtcc ccaatagccg ggaccatatc caatcccata cccgggcccg
    14881 tatccgggac cgggccagta ggaataggac tgactgccgg catagccggg atccaccaga
    14941 aagtcactct gcgccaaccg ctccgtctga tgaaccgatt gcgcacaacc cgccaggagc
    15001 aggatgacgc agagcacgat gaaccaaggt accaatcgag tcatggctgt ttcctcaacg
    15061 actgaccacg tcatcctcct tcattcagac acagacgatc gcaccggatc aatacttctt
    15121 ctgaaactgt ggcggcgcat tggccggcgg ggccggacgt ggtgcgggtg cgttcgactg
    15181 ctgactgccg atgccatagc cgcccccata ccacccatag ccggaaccat agtagccgaa
    15241 cggattccac cagaatccag ggccgttcca ataggacggt gattcgggat aaccgggatt
    15301 catgtgggcg cccacagatc caagacgctc tccctggtga aacgattgcg cacaggccgt
    15361 caggagcaac agagcgcttg cgacgaagag ggaaccgatc gatcgcgtca tggctgcacc
    15421 tcctaaaaat gataggacag gcccacaaag agttgatgtg cacgataggt cgcatcaaag
    15481 ccgccgaccg gtccgaacgc cccgccgaaa cgaaacgtag agtccgtata tttgtattcg
    15541 gtgaacaacg ccaccttcgg cgtgacgaat gctcgaattc cgacgaggac attcccgccg
    15601 accgacactt gcgtatcact ctccgtggtc gaggagcggc cgagtcgcgc gacaagcaac
    15661 gtctgccccc cgccgatata gggctgccag gtcacgccgg gatagcgcaa caccacatgg
    15721 gcaccgatgt tggttacgga gagatgggtc cccggcacat cgtcgaggtt cttgatatgc
    15781 ggggtactgt gcgagacgtc cagctcaatc ccgaacaatc catggccggg aaagacaccc
    15841 agcttgcctc cgaaggcagg gcttgttttt agattgaaat tcggagcatc cagaccagcc
    15901 agggtcccgc tccctcggac attccggagg ggatcggcaa agtttgcccc aagctgcgcg
    15961 gccacatacc attcagcgcg gatcggtgag ggagagcaca atagaagcac gaagcccaac
    16021 aacccggcac tcaacctgct gcacacaaac ctagtccaca ccatggtgct gactccatgg
    16081 gcgaggccta tctggatctt atcaggcaat ctgcgcggca gactggacaa ctttggtgtg
    16141 agcgaacgag ggatgtcgaa tgcgtcacgg acggaacgct attcgctcgg gtgctgggat
    16201 gttgagagag ttatttcgat ctcctgctac ggctcattgc gggcggagga attatagagc
    16261 aggtacaatg cgatcccgcc ggccacggca atgcccaccc ccgccagagc acgtgtaccg
    16321 aaactcagcg gccccacgac aaactccgct gcacgaggta atgccaccgt cgcgataatg
    16381 accagcagta acgcaagcag atgaatccag aggtagatgg gttcgccggg ttttcgcata
    16441 aatcagcgtg acgcttcacg cagtgtacga actctggccc cattattcca ctgtagaaca
    16501 aggggcgtca ggagcgcagg cctgaggctc aatcgagagt gaaccgcttc gtttcttcga
    16561 ggtgtttgct caaatatggc atgaagggcg tgccgccggt gccgacagcg gtgggattgc
    16621 tggcgtgcga ctggtgttgc cggaagatat accgctcggc gtacccgatg tgaatgtctc
    16681 gaaactgcgc gagtaaatcc acacaggtac aaaaggcttt ccatagatca ggatgcgtcg
    16741 ctttgcgatc gcgcgcatag ccgtagagca acgggcgatg ctgctcatcc gtgcgtgctt
    16801 cgagcgccgc cagaaacgtc cggtggcgcg gaggcatata gtcacgcatc tccacgaggt
    16861 actgcgtcag cgggtcgtcc gcatgaggga ttccaaggcc ggcatccagc gccggcacaa
    16921 tcgagctttg cgagccggtt tccccgcgga attgttgagg tcgctgcgcg tagtccgcga
    16981 ccccttcgta gacgactccc tgaggcaatg acgggctgtt cttccagcca tggatatagg
    17041 gccgcacgcg gctgtaatag atatacggat cacaacgctc gggcatgcga agcagcgtct
    17101 cccgcatcgc ttcctgcgcg gctgccagcg catggagtcc ttgcagcaca tcgtccggca
    17161 tattgtctcg cgcggcgttg tgggcttgaa ccaatcccac caaccccggt cctgccttcg
    17221 cctcaatatc gatatggatc aggacaaacc attcttcatc caggccccca agaaagttct
    17281 gcaggagcac cacgttgccc aactcgatgg gcttctgcgg atcgaggcgc cgccagttgt
    17341 gcagggcata ggaggcatag gacagcacgg gcggccggcc gagctgttgc gcaacggcac
    17401 accacggcag ggccagactg gccgggatga gtgacgcggg gtggtcggga tcttcccaga
    17461 caaaggcatg accggcaaag gagagggtgc gcatcgcaca acgaaagaca tcgagacccc
    17521 agtcatctgc caccggcccc atgacctctt gatggtcttg gacgaaagat cgaaattgtc
    17581 gcgccgtcag gaatttcgga agctccgcgc cgatctgatt caagatcggc tcatccggga
    17641 gctgcgccaa cgggtctgtc gccggaagga acccgcggtc aggtgaaaga tcgaactgcg
    17701 caagtgagag agactggtgg ctagacgtcg tcatggtcgc ttccacggga gacgcacaca
    17761 attcactcta ccacagtgcg gccatttcca gcgcagcacc gcactgcacc cggctcgcgt
    17821 gaatccaccg ggggactgaa aggcagttct tgacaagagc tctaactcgg gcgctcgcca
    17881 ccctcggctt tttcccgcac gttgaattga ttcatcgccg tggcgacatc gcggttgagg
    17941 agcaattcga gggcatcgac ggctcgctcc aggcagggga catagatcgc ccgctcttca
    18001 ggtgaaacct gttccagcac gtagtcggcc gtatcctgcc tcggtgctgg acggccgacg
    18061 ccgatcttca gccgcacgaa ctgcggcgtt cccaatgctt caatgacaga cttgatgcca
    18121 ttgtggccgc cgctgccgcc gtcttgcctg atccgcacac gacccggttc taaatccaga
    18181 tcgtcgtgca cgaccaccag ggcttccggg gtcagaccaa gctcgcggag gagccccttg
    18241 aggggagggc cactgagatt catccagtcg agggtactgg cgagttcgac gagtttggaa
    18301 ccggcgcgac ccgagcctcg ctgggccgcg cctttcctgg tgagccgtat cgaccaacgc
    18361 gcggccgccc gttcgatcac ccaacaaccg atattatgcc gtgtgtcggc atagtcggct
    18421 ccgggattgc ccaatccaac gagcaggcgc aacgcaacta cttcttcttt tccgcttcct
    18481 tcttcgcagg cgctgctccg gccttgggcg cggcagcccc agccttcgcg tctcccgcct
    18541 tgccggcctc cgcacccgcc gctccttcag ccaccgcctt gcccttcgcc gccacttcag
    18601 gctccttggc cccttcagca ccaggcgccc cggccgtgag caaagcttcg agcttggcat
    18661 cggtcatcgg cgcggccaca ctcacgacca tgtgctcaag gtcgtccaaa aaccgcaacc
    18721 cctcggtctt cggaatgtcc ttcagatgga tgccctggtt gatttggagc tgcgatgcgt
    18781 cgacttcgat gaaatcgggc aggacagacg ggagacattc gatgtgcagc tgacgcaaca
    18841 catgatgcaa caccccgcct tctttcagcc caagcggctg accgccggtc aagtgcaccg
    18901 gaactttgac gcgaatcggc ttatccatcg ccacttcaaa gaaatccgcg tgcaggacgc
    18961 tgcccgtaat cggatcgacc tggaattccc gcagcaacgc ggtacgcttg gccttgctct
    19021 tcgcgccgtt gatggcgagg gaaatgagcg cggtggatcc ggcttgagag cgcagaattt
    19081 tcaccaactc atcgggcttg actgagagca ggaggcattc cccctgcccg tacaacacgg
    19141 ccgggacccg acctgcacga cgaagttgac gcgcgggccc cttgccccca ccctctctgc
    19201 tttcaacggt taaatcgaat ttcatgatac tcctcctcga ccagtcgctc gacgcggacg
    19261 gcctacacga ataacgaact tactgattca tcttcatgga tacgtcgaat agcttccccg
    19321 aagagaggcg ccaccgacaa caccttgagt ttcggacact tctgttcctt gccacgcagg
    19381 ggaatcgaat cggtcaccag cacttccgtc agaccggact gttggagccg ctccaatgcg
    19441 ggccccgata acacgccatg tgaacaaccg gcccacacct cacgcgcgcc tttatccagg
    19501 caggcctg
//
LOCUS       DAOQRM010000032        19491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1938_length_19491_cov_14.122402, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000032 DAOQRM010000000
VERSION     DAOQRM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19491
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1938_length_19491_cov_14.122402"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..639
                     /locus_tag="PKK81_04600"
     CDS             82..639
                     /locus_tag="PKK81_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32179.1"
                     /translation="MTGLLTGCGGLQEMWEGPAARGFYPKTMAILPISGTYDSAREDV
                     EEVVAKVLIKSRRVEKVVPPDQVTDTFQTTKDAFDALVTYFSRMETTGQSDKNSAIKI
                     GHALGADSLLVVKVNAWEYTREEGDNIARVGLSMRLVDAVNGAIVWKARHQVQESYLF
                     IRPDMRDLATKLATDMIKYMPPEKR"
     gene            complement(642..1715)
                     /locus_tag="PKK81_04605"
     CDS             complement(642..1715)
                     /locus_tag="PKK81_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV32180.1"
                     /translation="MAVARRKVSRTNRKTTSKTASVAPRRKATARVPAAMGEERIIRT
                     LQQGLFDTLSGSEGEGRSPISVSAAFVQSFLQLTKVQAIAVYVRDEQTREMTCLAEAG
                     SSDASVAGKWREGLARAEQNAQVSSGDLYGLRLHRIGRMDGVVMFQTGKSQRLPSRKL
                     LTVLTAVEPWLAVLLDHARLTVKYAAKILRIQHMEQVSDLLNSSLAEEEKLRRALDAA
                     VRLVEAEAGALFLSAGDGALTLCTVAGERVTGLSALHSSIATGVHRTGQAVLITRGEQ
                     DARLVAGQGWQAALPVASLVSVPVRMGTQAVGVLEVVNRRSGKPFSNWDVLELASLSN
                     QFGLAIDNLRRGTVGEGGPKRVG"
     gene            complement(1918..2718)
                     /gene="trpA"
                     /locus_tag="PKK81_04610"
     CDS             complement(1918..2718)
                     /gene="trpA"
                     /locus_tag="PKK81_04610"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNV32181.1"
                     /translation="MNRLDHTLGQLKAKGEKALITYIMAGDPSLQETEQLVLELERAG
                     ADVIELGVPFSDPIADGPVIQKAAERGLQSGTSLRRILESVRHLRTSTQIPIVLMAYY
                     NNIHAFGEAEFCRIAADAGVDGLIVPDMPPDEAGPLRGPAGEAGLHLIFLLAPTSTAS
                     RRAYVAKESGGFVYYVSITGITGAKLQDVAGVRENVAKIRKHTKTPVAVGFGVATPED
                     AARVAQVADGVIVGSAIVRRVGEHGQDGRLVQEVGAFVRSLKEAMQPG"
     gene            complement(2884..4074)
                     /gene="trpB"
                     /locus_tag="PKK81_04615"
     CDS             complement(2884..4074)
                     /gene="trpB"
                     /locus_tag="PKK81_04615"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNV32182.1"
                     /translation="MAIPDRHGRFGPYGGRYVPETLMPALLELEEVYQRTRRDKGFQA
                     ELKHYLKEYVGRPTPLYFAQRLTKRLGGAKIYLKREDLCHTGAHKINNAIGQALLARR
                     MKKPRLIAETGAGQHGVATATVAAMFGLECEIYMGTEDMQRQALNVFRMRLLGSTVTG
                     VDAGSRTLKDAISEAMRDWTTNVRTTHYVLGSVLGAHPYPMMIRDFQSVIGREARKQI
                     LAAEGRLPDCLIACVGGGSNAMGLFHAFVPDKKVRMVGVEAGGLGVESGKHAARFAGG
                     RPGVLQGTMTYLLQDENGQVNLTHSVSAGLDYAAVGPEHSYYKETNRAQYTSVTDDEA
                     LEAFDLLAREEGIMPALESAHAIAEVVKCAPTMKKSQILIVNLSGRGDKDVQQVARIK
                     GVTL"
     gene            complement(4122..4754)
                     /locus_tag="PKK81_04620"
     CDS             complement(4122..4754)
                     /locus_tag="PKK81_04620"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNV32183.1"
                     /translation="MAVKVKICGLTNAEDAAVAVEAGADAVGFVFHKKSPRCAEPAAV
                     KAIVKELPPFVLPIGVFVNEDAKVVRDIMDSCGLALAQLHGDETAAYCEMLGRPVLKA
                     IRLRDRCSFLALAEFQGRAGVRGFLVDAFSPDAYGGTGQVADWALAAEAATVARILLA
                     GGLTPGNVAQAIEQVRPYGVDVSSGVEASPGRKDHDKVRAFVQAVRLVSR"
     gene            complement(4759..5073)
                     /locus_tag="PKK81_04625"
     CDS             complement(4759..5073)
                     /locus_tag="PKK81_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32184.1"
                     /translation="MKTRLSLGVMAALLTLAGCMNQEIHEYSPKWDAWMGSSKDDRIK
                     DMGIPTKCHSFKDGGEVCEWSVPQQDGRQDLIGLTFNAKGQACQWSYRGFYGMQKSKQ
                     SC"
     gene            complement(5070..5894)
                     /gene="trpC"
                     /locus_tag="PKK81_04630"
     CDS             complement(5070..5894)
                     /gene="trpC"
                     /locus_tag="PKK81_04630"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNV32185.1"
                     /translation="MILDRILEHKKAEIRHKNSRGYLADLKTRIRDAGPTLGFAVNLD
                     ARRTRTRPALIAEVKKASPSLGLLRPEFEQRFEPVAIAEAYREHGAAAVSVLTDKEFF
                     QGSLEYLSDVKRKVGLPALNKEFMVADIQFYEARAYGADAVLLIVAGLEKRQLIDFSA
                     LAKELSLDVLVETHHERELDTVLEWLPDVRLIGINNRDLKTFSTDLGVTLRLAKRIPG
                     DKLIVSESGIHKREDVTRLVEAGVHAMLIGESLIRAQDIGVKIRELLGDESNKEKP"
     gene            complement(5899..6915)
                     /gene="trpD"
                     /locus_tag="PKK81_04635"
     CDS             complement(5899..6915)
                     /gene="trpD"
                     /locus_tag="PKK81_04635"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNV32186.1"
                     /translation="MIKDAINTLAERSDLTEQEAETVMGEIMDGAATSAQIAGYLMGL
                     RMKGETVAEIAGSVQAMRARATKIRVRDSQVVDTCGTGGDRAHTFNISTTAAFVAAGA
                     GLTVAKHGNRSVSSKSGSADVLAALGVAIDLPPERVADCVNEVGIGFLFAPLYHAAMK
                     HCAQPRQELGIRTLLNILGPLTNPAGARIQVVGVFDAGLTELLAKVLLYLGAQHCFVV
                     HGMDGLDEITVTDRTRISEGKAGVVSSYTIDPTEFGLTRVRPKELVGGSAEDNAAITR
                     DIFRGRKGPKRDIVCLNAAPALVAGRKAKTLQEGFQLAQQTIDSGAAMEKLDQLIAFT
                     KKAS"
     gene            complement(7002..7565)
                     /gene="pabA"
                     /locus_tag="PKK81_04640"
     CDS             complement(7002..7565)
                     /gene="pabA"
                     /locus_tag="PKK81_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNV32187.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVVVYRNDKISIQEIEALKP
                     RRLVISPGPCTPNEAGISVEAIRHFGGKLPLLGVCLGHQSLAVAFGGEVIRAERLMHG
                     KTSMVRHDGRTIFRNLPNPFEATRYHSLIVNRKNLPDCFEISAETAEGEIMGMRHKML
                     GIEGVQFHPESILTTAGKELLRNFLKL"
     gene            complement(7656..9152)
                     /gene="trpE"
                     /locus_tag="PKK81_04645"
     CDS             complement(7656..9152)
                     /gene="trpE"
                     /locus_tag="PKK81_04645"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNV32188.1"
                     /translation="MSRQQYSLTLDEFRALAAEGNLIPLYREILADYETPVSAFAKID
                     HGPTAYLLESVAGGENWARYSFLGSGSSAVIREEKGDLVLIRGKKNLRIQSRGNPLER
                     LRELMEEYRPVTVPGLPRFVGGAVGYFSYDMVRTFEDLPALRKDSLGMPDFAFLLTDT
                     LLIFDNVSQKIKVVANAYLESTKERDIRDAYRHATARIEKMIARLKRPVRQPRQRRRR
                     KPITFTSNMNKADFEKMVTRTKEYIRSGDIVQAVLSQRWETQIHTTPFQLYRALRVIN
                     PSPYMYYLRVAGVELVGSSPETLVRCEDGQISLRPIAGTRRRGQTPEEDQDLARQLLA
                     DEKERAEHVMLVDLGRNDVGRVAARGSVKVESLMQVERYSHVMHIVSQVTGQLEKGKS
                     VYDVMRACFPAGTVSGAPKIRAMEIIEELEPTRRGPYAGAVGYFGFSGNMDMCINIRT
                     VVIKGRQAYIQAGAGIVADSIPEHEYEETCNKARAMMKAIELAEQGLE"
     gene            complement(9173..9871)
                     /locus_tag="PKK81_04650"
     CDS             complement(9173..9871)
                     /locus_tag="PKK81_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNV32189.1"
                     /translation="MMSQRNSRMRRAVVVPIGLLVLSGLNLGGCVMSEKYEAEKARSL
                     NFQRLLAQEEKRSAELDSEVKRSKRELSEYEARNRELGAQVEAVRQQAAQIQEEAQAM
                     KEASLLEKRAHEDMKRLTTIPSKTKKAAAAAKKDFTAAVDEALKTDVSSELSLESAKD
                     AAKDTFGHEQAAVDALGAASASATGNTHTVRSGETLYRIGQKYHVGVDQLRKWNNLDN
                     NTVVVGQKLIVSRP"
     gene            10109..11062
                     /locus_tag="PKK81_04655"
     CDS             10109..11062
                     /locus_tag="PKK81_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV32190.1"
                     /translation="MSPNTQPGTSRTVTIEGITLHLAQPMTMDQEWIGNREILKQLLA
                     CWLVIDERDLPLSPRITGQPGIGKTTLAMAGARERKQDLYVFQCTADTRPEDLLITPV
                     LAESGTISYHASPLITAVLTGSICVLDEGNRMNEKSWASLAPLLDHRRCVESIIAGIL
                     INAHAEFRCCVTMNEDASTYEVPDYILSRLQPTLGMGFPAREDELAILRYHLPFAPAE
                     MLAMTVDFLQEAHQLSLEYSVRDGIHLLQYALKRRAQDPTHPLSADAAWRESLMKVLG
                     EEALDLPTQSRRRKRALGDQALPQGLGDFFFESDDPLHPGR"
     gene            11062..12960
                     /locus_tag="PKK81_04660"
     CDS             11062..12960
                     /locus_tag="PKK81_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32191.1"
                     /translation="MAVSSSTADSVFRLSPRIELFPLLHGSGDVAQEVRERLTDRRFD
                     CLAVPLPPSFEHPLEEAVMDLPTISVIVQPERDQEGAATVNYVPVDPCQAVVMGIRVA
                     MGEGIPRAYIDRETAVVEPVPFVSPDAYALKRLSYATFAAAMVPTLTPPAEGSQQQAR
                     IAWMAFQLHQLEMEYEQILCLCHFADWPWIRQAYQQRTDYQIPALDFGRPERYLVEPA
                     SLYFVLGELPFLTELYERRRATVHSDRHLSIDGIKELLLETRTRWQTGHDAEGQTVPT
                     WVTPQLLQAYLQYVRNHALLDRRLTPDLYTLVLAAKQMAGDDFAVRLLETAKSYSYQE
                     IEQSHVPHLSAGLGRFALPDEQIVRAKNRLQGTPLAWRSLSLRPRPPRRTSRQWSYLW
                     NPHRQCSWPPEDSKIESFHTHVREQAKAIIGADLVKSEKFTTSMKDGIDIRESLRHWH
                     QRQKPGRPQRMEIYVKEIPPARGNVDTVIFLFDTPGDPEQYSWQATWYAEHAQESTLC
                     FYATPFLNNMIAPGIGQSRYGGALFMFPPRPIPDIWTDEALGFARTLEERLIAAGAVH
                     SRETHVALVTPVPPKARWRQIARQFDRRLVPIPLSRFSGQMIDRLRRFHVLNGHEIRS
                     FAAQFIRE"
     gene            12968..14980
                     /gene="ligA"
                     /locus_tag="PKK81_04665"
     CDS             12968..14980
                     /gene="ligA"
                     /locus_tag="PKK81_04665"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNV32192.1"
                     /translation="MPHQTAEQRIADLRRQIRHHDHLYYTKDRPEISDAEYDRLFREL
                     VDLETAHPDLVTGDSPTQRVGAPPLEELTKVSHEKPMLSLDSITDQDDVRAFDARMKR
                     ELETDQIVYTAEPKFDGLSVELVYDRGAFVRGATRGDGMVGEDVTINLRTIRALPLQL
                     DPQPSLPAHLVVRGEVYMRLDEFQVLNRRMTERGEEAFANPRNAAAGSLRQLDSRITA
                     SRPLTLTCYDIMALSGTAPPTHWEELDALAAWGLPVPEHRQRCRSIEEVLSFHAGTDG
                     MRDSLPFEIDGIVVKLDRRTWQDQLGSKSRSPRWAIAYKFAPRKEITVIQDIAVSVGR
                     TGTLTPLALLKPVEVGGVTISRATLHNADEVARKDVRVGDTVKVERAGDVIPAIAERV
                     PVPGETRQAPFVMPDHCPVCGSAVAREGAYYYCTGQTVCVAQLKGAIEHFASKSALDM
                     DGMGKKTIAQLVDVGLVRDLADLYSLTKEQLLSLEGFADRSATLLMESIERSKSTSLD
                     RLLMGLGIRQVGQHIARVLAKQFGTLAKLMSATQEELLQVREIGPEISTSLASFFSEE
                     RNRAVIARLIERGLTIAPPSSEIAAGSQSLAGKTFVFTGGLANYTRDQAKQLVEQQGG
                     QVSSSVSKKTSFVVAGTDPGSKLDQARKLGVRVLTETEFTALVTPE"
     gene            complement(14955..16268)
                     /locus_tag="PKK81_04670"
     CDS             complement(14955..16268)
                     /locus_tag="PKK81_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV32193.1"
                     /translation="MDIIVLIVLILLSAVISVVEVGFYSVNDTKLRALADTGSKRAEM
                     ALHLRTDPQRLLLTILVGDRLIDTATASLATIIALNRFGGQGLEGVLGEAFAVLVGIL
                     TFVLLVFADLVPKTLAAKYSVPVVLNMAYPAYAAQQVLTPIMFFVVPLIYKLTGGKGL
                     NVPFVTEEELKIMLDQSSKSGAIEAQEVKMIKNVFQLKDITAEDCMTPRIYMFSLDCN
                     QYLREAKELLFKSKYSRIPLYEGTLDNIIGILYKTKALTALAQGHTEMKLRDIAQPAL
                     FIPHTKSADDLMKQFQLDKRHMAIVVNEFGGVMGLVTLEDLLEEVVGEIVDETDITEE
                     LIKRIGKNQILVHGRTEVRKVNDFLKVDLGDDAVTISGLVQHELGRIPKVGEEVHIAN
                     CRLVIHEADPRVIKSVNIYKEEKHPITHEPIVDEHVSVTQASLER"
     gene            complement(16536..17243)
                     /locus_tag="PKK81_04675"
     CDS             complement(16536..17243)
                     /locus_tag="PKK81_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNV32194.1"
                     /translation="MPGEKRTSTSRLLLPLFAFLLTTWLSGCSGWSPARPSYPPGYPL
                     GFIERGSASWYGPGFHGNRTANGEVYDMHKLTAAHRTLPLGSVAVVRSLSTGRQVTVR
                     INDRGPFARGRILDLSLAGAQAVGMVGRGTDEIELRVISYEGRAGGMGVLRVQVGSFA
                     DPANARTLVERLRSAYPGSKIVGVDLPDGRRYRVYAGQFQTEVAAEQAAAHLRRALET
                     DPFIVRDDAPGATTGNP"
     gene            complement(17252..17662)
                     /locus_tag="PKK81_04680"
     CDS             complement(17252..17662)
                     /locus_tag="PKK81_04680"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNV32195.1"
                     /translation="MPTTTQFVVSGQSKPGVLAEVAAVLGAAGVNIKAFSAPEVTGPG
                     KLRLIVADVDTARIALRKSKIKFREETALILSLENKPGALKQVADSLTRARINVKCGY
                     CTPSREGKRAIVVLTVSNTTKALGVLRTHSLDEF"
     gene            complement(17702..17992)
                     /locus_tag="PKK81_04685"
     CDS             complement(17702..17992)
                     /locus_tag="PKK81_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV32196.1"
                     /translation="MRRTMVMVDQRAVAREWQARGFSCDLWVDPPGQVWEDYRHATDE
                     LVMPVEGRLELEFGGRCVRPEPGEEILIPAGVSHTVRNIGGTSVKWLYGYSR"
     gene            complement(17997..19109)
                     /gene="fbp"
                     /locus_tag="PKK81_04690"
     CDS             complement(17997..19109)
                     /gene="fbp"
                     /locus_tag="PKK81_04690"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HNV32197.1"
                     /translation="MAAESTQSITLSVIKADIGGFVGHSAMHPALMDCAKEKLAVAKT
                     SGLLVDYHVSACGDDLQLIMTHRQGVDHEGIHRLAWDTFEAGTAVAKELHLYGAGQDL
                     LADAFSGNVRGQGPGVAEMEFVERKSDPVLIFMADKTSAGAWNLPLYKMFADPFNTAG
                     LVIAPTLHQGFRFEVHDIYEHKKISFDCPEELYDMLMFIGSPGKYTIKYVYSRADGTI
                     AAASSTERLSLIAGKYVGKDDPVMIVRAQQNFPAVGEVLDPFRYPWIIEGWMRGSHYG
                     PLMPVSVKQATPTRFDGPPRVACLGFQLADGKLVGPRDMFDDPSYDEARRDANRIADV
                     LRMHGPFEPHRLPLDDMEYTTMPQIMKKLEGRWAKV"
BASE COUNT         3944 a         6404 c         5306 g         3837 t
ORIGIN      
        1 ctgaatcaat ctgctacact tgctatatca accatgacac gacacacagt acgcactatc
       61 agtgttgttt tctgcctcgt gatgaccggc ctcctgaccg gctgcggagg cttgcaagaa
      121 atgtgggaag ggccggccgc gcgcgggttc taccccaaga ccatggccat tctcccgatt
      181 tccggcacct acgatagcgc ccgggaagac gtcgaagaag tggtggcgaa agtgctgatc
      241 aagagccgtc gcgtagagaa ggtcgtcccc cccgatcagg tcaccgatac cttccagacc
      301 acgaaagatg cgttcgatgc gctcgtgacc tacttctcac ggatggaaac gacaggccaa
      361 tcggacaaga attccgcgat caagatcggg cacgcgttgg gtgccgactc tctgctggtg
      421 gtcaaggtga atgcctggga atatacgagg gaagaaggcg ataacatcgc ccgcgtgggc
      481 ctgagcatgc ggctggtcga tgccgtgaac ggcgccattg tctggaaagc gcgccaccag
      541 gtgcaggaaa gttacctctt catccgtccg gatatgcggg atcttgccac gaagctggca
      601 acggacatga tcaagtacat gccgccggaa aaacggtagc cttagccgac ccgtttcggc
      661 ccgccctccc cgacggttcc tctgcgcaaa ttgtcgatcg ccaatccgaa ctgattcgag
      721 agactcgcca gttccagcac atcccagttg ctgaacggct tgccgcttcg tctattcacc
      781 acctccaaca cacccacggc ctgcgttccc atgcggacag gcacagagac gagggacgcc
      841 accggcaagg cggcttgcca tccctgaccg gcaaccaatc gggcatcctg ctctcctcgc
      901 gtgatcaata ccgcctgacc tgtgcgatgc acgccagtcg cgatggacga gtgaagggcc
      961 gagagacccg ttacgcgctc gccggcgacc gtgcagagtg tcaatgctcc atcaccggca
     1021 ctgaggaaca acgccccggc ctccgcttcc accaagcgaa ccgcggcatc caacgcccgg
     1081 cgtaacttct cctcttcggc cagcgacgaa ttcaaaagat cgctcacctg ctccatatgc
     1141 tgaatccgaa gaatcttcgc cgcgtacttg acggtcagtc gcgcatggtc cagcaggacc
     1201 gccagccagg gctcgactgc cgtcaacaca gtcagcaact tccgcgaggg caaccgctgg
     1261 gacttcccgg tctgaaacat cacgacgccg tccatccggc cgatccggtg caatcgtaaa
     1321 ccatacaagt cgccgctgct cacctgtgca ttctgttccg ccctggccag gccctcccgc
     1381 catttcccgg ccacagaggc atcgctgctc ccggcctccg ccagacaggt catctcgcgg
     1441 gtctgctcat cacgcacgta cacggcgatc gcctgaacct tcgtcagctg caggaacgat
     1501 tggacaaatg ccgccgagac gctgatgggt gaacggccct ccccttcgga accggacagg
     1561 gtatcgaaca atccctgttg cagggtgcgg atgatgcgct cttcacccat tgccgcaggc
     1621 acacgcgccg tagctttgcg gcgtggagcg accgatgcgg ttttcgatgt cgtcttgcgg
     1681 ttggttcggc tgaccttacg acgagctacg gccatggcgt ctcctcatgt gaagcgtcaa
     1741 acgttgcacg gcagggaaca tctccgctgc cacgaattcc ttggatcagg acaatacgca
     1801 accaggagcc gccgtcccgg tcggaacgac cgggcggaca ggactccaca ggaatcatgt
     1861 acgaagatgt tggctgaaaa gttgaacaga atacggaggg gggagaagag ctgccggcta
     1921 gcccggctgc atcgcctcct tgagggatcg cacgaacgca cccacttcct gaacgagtcg
     1981 gccatcctgc ccatgctctc caacacgacg cacaattgcg ctgcccacga tcacgccatc
     2041 ggcgacctga gcaacccgcg cggcatcctc cggagtcgcc acgccgaatc ccacagccac
     2101 cggggtcttg gtgtgctttc tgatcttggc gacgttctcg cgtacgcccg ccacgtcctg
     2161 tagtttagcc ccagtaatgc ccgtgatgga aacataatag acaaatccgc cggactcctt
     2221 cgccacatag gcccgcctcg acgcagtgct ggtcggagcc aacagaaaga tcagatggag
     2281 tccggcctca ccagccggac cacgcaacgg ccccgcctca tccggcggca tatccggcac
     2341 gatcaatcca tctacacccg catccgcagc gatccggcag aattccgcct caccgaacgc
     2401 gtggatgttg ttgtaatagg ccatcaatac gatagggatc tgggtcgagg tccgaagatg
     2461 ccgcaccgat tccagaatcc ttcgaagcga cgtaccgctt tggagaccac gctcggctgc
     2521 tttctgaatc accggtccat cggcgatggg atcggaaaag ggcaccccca attcgatgac
     2581 gtcggcgccg gcccgctcca attcgagcac caactgctcc gtctcctgca acgacgggtc
     2641 ccccgccatg atgtaggtga tcagcgcctt ctcccccttc gccttcaact gccccaatgt
     2701 gtgatccaaa cgattcatat ggatatacct atgcctctcg ctcgtccttc caatcaattg
     2761 caggaggttc aaaacggtca tccagcgcgg ccgcagcaag cgcggaggtg aggcgtactc
     2821 ctgcagtacg tcgaacctgt gcgcgccgcg agaacaaagc tggaggccgt tctcaacctc
     2881 ctgctacagc gtcacgcctt tgattctcgc cacctgctgc acatccttgt cgccgcgccc
     2941 ggaaaggttc acgatcagga tctggctctt cttcattgtg ggcgcgcact tcaccacttc
     3001 ggcgatggca tgggcgcttt ccagcgcggg catgatgccc tcctcccgcg cgagcagatc
     3061 gaacgcctcc aacgcctcgt catccgtgac cgacgtatac tgggcacgat tcgtttcctt
     3121 gtagtagctg tgctccggcc ccacggccgc atagtccaag cctgccgaga cggaatgggt
     3181 gagattcacc tgaccgttct cgtcctgcag cagataggtc atggtccctt gcaacacgcc
     3241 ggggcgaccg cccgcaaacc gggccgcatg tttgccgctc tcgacaccca gccccccagc
     3301 ttccactccc accatcctga ccttcttatc cggaacgaaa gcgtggaaca gccccatcgc
     3361 attactgccg ccgccgacac aggcgatcag acaatcgggc agacgccctt ccgcggccag
     3421 aatctgtttg cgggcttcac ggccgatgac ggactgaaaa tcccggatca tcatgggata
     3481 gggatgcgcg ccgaggacgg aacccagcac atagtgggtc gtccgcacgt tggtcgtcca
     3541 atcccgcatc gcttcactga tggcgtcctt caacgtccgg ctgccggcat ccacgcctgt
     3601 gaccgtcgaa cccaggagcc gcatccggaa cacattcagc gcctgccgct gcatgtcttc
     3661 cgtgcccatg tagatttcac attccaggcc gaacatggca gccacggtgg ccgtcgccac
     3721 gccgtgctga cccgcgccgg tctctgcaat gaggcgcggt ttcttcatgc gccgggcgag
     3781 cagcgcctgc ccgatcgcat tgttgatctt atgggcgccg gtatggcaca ggtcttcgcg
     3841 cttgaggtaa atcttcgcgc cgcccaatcg tttcgtaagg cgctgggcaa aatacagcgg
     3901 cgtcggccgc ccgacatatt ctttcaaata gtgcttgagt tcggcttgaa accccttatc
     3961 ccgtcgtgtg cgttggtaca cctcttccaa ctccagcaag gccggcatga gagtttcagg
     4021 gacataccgt ccgccgtaag ggccgaaccg cccatgtcga tctggtatcg ccatgtgaat
     4081 tccttcagct agtcgtgaaa aatgaaagta tagacgctga cctagcggga gacaagcctg
     4141 acggcttgca caaaagcccg gaccttgtca tggtctttcc tgcctggcga ggcctcgact
     4201 ccgctgctga catcgacgcc gtagggccgc acctgttcga tggcttgcgc gacattcccc
     4261 ggagtcagtc ctccggccag cagaatacgc gccaccgtgg ccgcttcagc agccagcgcc
     4321 caatcggcca cctgtcccgt accgccatag gcgtcgggag aaaaggcatc caccagaaat
     4381 ccccgcacgc cggcgcgccc ttgaaactcg gcaagcgcca gaaatgaaca tcgatcccgg
     4441 agacgaatcg ccttcagcac cggccgcccc agcatctcgc aataggccgc cgtctcatcg
     4501 ccatgaagct gcgcaagggc aagaccgcaa ctgtccatga tgtcccgcac caccttcgca
     4561 tcctcattca caaacacgcc gatcggcaac acaaacggcg gcagttcctt cacgatggcc
     4621 ttgaccgccg ccggttccgc gcaccgaggg ctctttttat gaaacacgaa cccgaccgca
     4681 tccgcgccgg cttccaccgc cacggccgcg tcctcggcat tcgttagacc gcagatctta
     4741 acctttacag ccatggcgtc agcagctctg cttgctcttc tgcatgccgt agaacccgcg
     4801 ataggaccac tggcaggcct gccccttggc gttgaaggtc aggccgatca agtcctgccg
     4861 cccgtcctgc tggggaacag accactcgca cacttcgccg ccgtccttga acgaatggca
     4921 cttcgtgggg atgcccatgt ccttgatgcg gtcgtctttg ctgctgccca tccaggcatc
     4981 ccacttcggg gaatattcat ggatctcctg attcatacaa ccggccaagg tcagcaacgc
     5041 cgccatcaca ccgagagaaa gtcgcgtctt catggcttct ccttgttgga ttcatcgccc
     5101 agcaactcac gaatcttcac gccgatatcc tgagcccgga tcagcgactc gccgatcagc
     5161 atggcatgga caccggcctc caccaatcgc gtgacatcct cccgcttgtg aatcccgctt
     5221 tcactcacga tcagcttatc gccgggaatg cgcttcgcca gtcgcagggt cacgcccaga
     5281 tccgtcgaga aggttttgag gtcacgattg ttgataccga tcagacggac atccgggagc
     5341 cattccaaca cggtatcgag ttcacgctca tgatgcgtct ccaccagcac atcgagcgac
     5401 aactctttcg ccaatgcgga aaagtcgatc aattggcgct tctccaaccc ggccacgatc
     5461 aacaggaccg cgtccgcgcc ataagccctg gcttcataga actgaatatc cgccaccatg
     5521 aactctttgt tcagcgcagg caggccgact ttccgcttca catcggagag atactccagg
     5581 ctgccctgaa aaaattcctt atccgtcaat acggagacag ccgcggcccc atgctcccga
     5641 taggcttcgg cgatggccac cggctcgaag cgctgctcga actccggccg caacagaccg
     5701 agactcggag aggccttctt gacctccgcg atgagcgcgg gcctggtccg agtccggcgc
     5761 gcgtccaggt tcacggcaaa ccccagtgtc gggccggcat cgcgaattct ggttttcaga
     5821 tccgcgagat agccacggct gttcttgtgg cgaatttccg cttttttatg ttcgagaatc
     5881 cgatcgagaa tcacgatgtc acgacgcctt cttcgtaaat gcgatgagct gatccagttt
     5941 ctccatcgcc gcacccgagt cgatcgtctg ctgggcgagt tgaaaccctt cctgcagcgt
     6001 cttcgccttg cgtccggcca cgagcgccgg tgcggcattc agacacacaa tgtcgcgctt
     6061 cggtcctttc cgtccccgga aaatatcgcg cgtgatggcg gcattgtctt ccgcactccc
     6121 accgaccagt tctttcggcc tgacacgtgt gaggccgaat tcggtcggat cgatggtata
     6181 actcgacacc acgccggctt tgccctctga aatccgggtc cggtccgtga cggtgatctc
     6241 atccaatccg tccatgccgt gcacgacaaa acaatgttgc gcgcccaggt agagcagcac
     6301 cttcgccaac aactccgtca agccggcatc gaataccccg acgacctgta tgcgggcacc
     6361 ggctggattg gtcagggggc cgagaatatt cagcagtgtc cgaatcccca gttcttgccg
     6421 cggctgggca cagtgcttca ttgcggcatg atagagcggc gcaaagagaa acccgatacc
     6481 cacctcgttc acacagtcgg cgacccgctc aggcggcaaa tcgatcgcca cacccagcgc
     6541 ggccaacaca tcggcactgc ctgacttgga cgagaccgac cggttgccat gcttcgccac
     6601 cgtcagcccg gcgcccgccg ccacgaacgc cgcagtagtc gaaatattga aggtatgcgc
     6661 ccgatcaccg ccggtcccac aggtatctac gacctgagaa tcccgcaccc ggattttggt
     6721 cgcgcgagct cgcatggcct gtacggagcc ggcgatctcg gccacggtct cccccttcat
     6781 ccgaagcccc atgagatatc cggcgatctg ggcggaggtc gccgcgccgt ccatgatctc
     6841 gcccatcacg gtctcagcct cttgttcggt gaggtccgac cgttcggcca atgtattgat
     6901 tgcgtctttg atcatgaggg gcgcgatcag cgatccgcgg tcggtaatca gtcggaaggc
     6961 ctcatagaga gcactgattg ccggtggccg acagccgtgg attagagttt caagaaattc
     7021 ctgagcagct ccttgccggc ggtggtgaga atcgactccg gatggaattg caccccttcg
     7081 atgcccaaca tcttgtggcg catccccatg atctcgcctt cggcggtctc ggcgctgatc
     7141 tcgaaacagt ccgggagatt cttgcgattc acgatgagag agtgatagcg ggtggcctcg
     7201 aacggattcg gcagattgcg gaaaatggtg cgaccgtcgt ggcgaaccat ggacgtcttg
     7261 ccgtgcatga gacgctcggc gcggatcact tcgccgccga acgcaactgc cagcgattgg
     7321 tgaccgagac agacgcccag cagcggtaat ttgccgccga aatgccgaat cgcttccacc
     7381 gagattcccg cctcgttcgg cgtgcagggg ccgggcgaaa tgacgagccg gcgcggcttc
     7441 aacgcctcga tctcctggat ggaaatcttg tcgtttctgt agacgaccac atcttccccc
     7501 aactcgccga aatattgcac gaggttgtag gtgaaggaat cgtaattatc gatcatcaac
     7561 aacatatgga ccactcgtcg ctcgtcgttc gtgaatcgta tctcgtgggg tcgccgttcc
     7621 tgcgtttcac gtctgacctt tcacgtttca cgcgctcatt ccaaaccctg ctcggcgagt
     7681 tcgatggctt tcatcattgc ccgcgccttg ttgcaggttt cttcgtattc atgttcagga
     7741 atcgaatcgg cgacgatacc ggctcccgct tgaatatagg cctgccgccc cttgatcacg
     7801 acggtccgga tgttgatgca catgtccatg ttgccggaaa atccgaaata gcccaccgcg
     7861 cccgcatagg gaccacggcg tgtcggctcc aactcttcga tgatttccat cgcgcgaatc
     7921 ttcggcgcac ccgacacggt ccccgccggg aaacaggctc gcatgacgtc gtacacggat
     7981 ttccccttct ccagctgtcc cgtcacctgc gacacgatat gcatgacgtg cgaataccgc
     8041 tcgacctgca tcagcgactc caccttcacc gagcctctgg cagccacccg tcccacgtcg
     8101 ttgcgtccaa ggtccaccag catcacatgt tccgctcgct ctttttcatc tgccaacagc
     8161 tgtcgcgcca ggtcctgatc ttcttccggc gtctgcccgc ggcgacgcgt cccggcaatg
     8221 ggacgcaacg aaatctggcc atcctcacac cgcaccaggg tctccggcga ggaccccacc
     8281 aactccactc cggccacccg caggtaatac atgtagggcg agggattgat gacacggagg
     8341 gcacggtata actggaacgg agtcgtatgg atctgtgttt cccatcgttg agacaggacc
     8401 gcctgcacga tatcgcccga acggatgtac tccttggtcc gcgtgaccat cttctcgaaa
     8461 tcggccttgt tcatgttgga cgtaaaggtg atcggcttcc ggcggcgtct ctgccgcggt
     8521 tgccgcaccg gccgcttcaa ccgcgcgatc atcttttcga tccgcgcggt cgcatggcga
     8581 taggcgtcac ggatgtcccg ttccttggtc gattccaggt aggcgttcgc gaccactttg
     8641 attttctgcg agacgttgtc gaagatcagc agcgtgtcgg tgagcagaaa ggcgaagtcc
     8701 ggcatcccga ggctgtcttt acgcagcgcc ggcaggtcct cgaacgtgcg caccatatcg
     8761 taactgaagt agccgaccgc gccgcccacg aaccggggca ggccgggcac cgtcaccgga
     8821 cgatattctt ccatcaattc gcgcaatcgc tccagcggat tgccgcgact ctgaatccgc
     8881 aggttcttct tccctcggat caacaccaga tcgcccttct cctcccggat caccgccgag
     8941 gagccgcttc ccaaaaacga ataccgcgcc caattttcac cgccggccac actctccagc
     9001 agataggccg tcggcccatg gtcgattttg gcaaacgccg acaccggcgt ctcatagtcc
     9061 gccagaatct cccgatagag cggaatcaga ttgccttccg ccgccagggc tcgaaactca
     9121 tccaaggtga gagagtattg ctgtctgctc atacaatacg acgatcgcta cactagggcc
     9181 ggctgacaat caacttctgc ccgaccacaa ccgtgttatt gtccaaattg ttccacttcc
     9241 gcaattgatc cacaccaacg tggtacttct gcccgatgcg atacagggtt tcaccggatc
     9301 gcaccgtgtg agtgttgccg gttgccgatg ccgaagctgc accgagggca tccactgccg
     9361 cctgctcgtg accgaatgta tccttggccg cgtccttcgc cgactccagg ctcaactccg
     9421 acgacacatc cgtcttcaac gcctcatcga cggcggccgt aaagtctttc ttcgccgccg
     9481 ctgccgcctt tttcgttttg gatggaatcg tcgtcaggcg cttcatatct tcatgcgccc
     9541 gcttctcaag caaactggct tccttcatcg cctgagcctc ttcctgaatc tgtgccgcct
     9601 gctgacgcac cgcttccacc tgagcgccga gttcccgatt gcgcgcctca tactcactca
     9661 gttcgcgttt gctccgtttg acttcgctgt ccaattcggc gcttcgcttt tcttcctgcg
     9721 ccaggagccg ctggaagttc aggctgcgcg ctttctcggc ctcgtacttc tccgacatca
     9781 cgcatccgcc cagattcagg ccgctcaata cgagcaaccc gatcggaact actaccgcgc
     9841 gtctcatccg tgagtttcgc tgactcatca tgtcgctcct tcgttgctgt cttctccagg
     9901 gggcttccga atgccatcaa aacaacccgc tcgtggcgca tccgcttccg attcgcagcc
     9961 ttcgaaagtg ggtagaatac ggtgcaaggc agcgaaagtc aaagtgaaat gggccgctca
    10021 gacgtttgac cccctcccca cccctatgct accgttcgca cgagattgct gttccgccta
    10081 ttctatccaa tcgccggcac gaaagaccat gtcacctaac acacaaccgg gcacctcccg
    10141 caccgtcacc atcgagggca tcaccctgca cctggcacaa cccatgacca tggaccagga
    10201 atggatcggg aaccgggaga ttctcaaaca gttgctggcc tgctggctgg tgatcgacga
    10261 gcgtgacctg ccgctgtctc cccgcatcac cggccaaccg gggatcggca agaccacact
    10321 ggccatggct ggagcccgcg aacgcaagca ggacctctac gtattccagt gcacggccga
    10381 cacccggccg gaagacctgc tgatcacgcc cgtcctggca gaatccggca ccatcagtta
    10441 ccacgcctcg ccgctgatca ctgcggtgct cacaggcagc atctgcgtgc tggacgaagg
    10501 caaccgcatg aacgagaaaa gctgggcctc gctggctccg cttctggatc accggcgctg
    10561 cgtggaatcc atcatcgccg gcattctgat caacgcccat gccgaattcc gctgctgcgt
    10621 gaccatgaac gaagacgcgt ccacgtatga ggtgcccgac tatattctct cgcgcttgca
    10681 gccgacgctg ggcatggggt tccctgcgcg cgaagacgaa ctggcgatcc tccggtatca
    10741 cctgcccttc gcgcccgcag agatgctggc catgaccgtc gactttctcc aggaggccca
    10801 ccagctcagt ctcgaatatt ccgtgcgcga cggcattcat ctcctgcagt acgcgctgaa
    10861 gcgtcgcgcg caggacccga cgcacccgct gtcggccgat gccgcctggc gggaatcact
    10921 gatgaaagtc ctcggcgaag aagcgctcga tctgccgact cagtcacgcc ggcgaaagcg
    10981 cgccctgggc gaccaggccc tgccgcaggg cctcggcgat ttctttttcg aaagcgacga
    11041 ccctctacat cccggccgat aatggcagtg tcatcctcca ccgccgactc cgtcttccgc
    11101 ctgtcgccac gcatcgaact gttcccgctc ctgcacggca gcggcgatgt cgctcaggaa
    11161 gtgcgcgaga ggctcaccga ccgccgattc gattgcctcg cggttccgct tccgccttcc
    11221 ttcgagcatc cgctggaaga agccgtgatg gacctgccga cgatcagcgt gatcgtccaa
    11281 ccggaacgcg atcaggaagg cgccgccacg gtgaactacg tgccggtcga tccctgccag
    11341 gcagtcgtca tggggattcg cgtcgccatg ggagaaggca tccccagggc ctatatcgat
    11401 cgcgaaacgg ccgtcgtgga accggtgccc ttcgtctctc ccgatgccta cgcactcaaa
    11461 cgcctctcct acgccacctt tgccgccgcg atggtaccca cgctcacgcc gcctgcggag
    11521 ggcagtcaac agcaggcccg catcgcctgg atggcgtttc aactccacca actcgaaatg
    11581 gagtacgagc agattctctg cttgtgccac ttcgcagact ggccctggat tcgtcaggcc
    11641 tatcagcaac gaaccgacta tcaaatcccg gcgctcgact tcggacgccc cgaacggtac
    11701 ctcgtcgagc cggcctcact ctattttgtc ttgggtgaac tgccgttcct cacagagctg
    11761 tatgagcgac gtcgcgcgac cgtccattcg gaccgccacc tctccatcga cgggatcaaa
    11821 gaactcctgc tcgaaacacg cacaaggtgg cagaccggcc atgacgcgga ggggcaaacc
    11881 gtccccacct gggtgacacc gcagttgctg caagcctact tgcagtatgt gcggaaccac
    11941 gcgctgttgg atcggcgatt gacgcccgac ctctacacgc tggtcctcgc ggcaaaacag
    12001 atggcaggtg acgacttcgc ggttcgcctc ctcgaaaccg ccaaaagtta ctcctatcaa
    12061 gagatcgaac agagccacgt ccctcatctc tcggccgggc tcggccggtt cgccctgccg
    12121 gatgagcaga tcgtgcgggc gaagaaccgg ctccagggta cgcccctggc ctggcgttcg
    12181 ctctcgctcc ggccccgccc gccgcggcgc acgagccggc agtggtccta tctgtggaat
    12241 ccgcatcgac aatgttcctg gccgccggaa gattccaaga tcgaaagttt ccacacccat
    12301 gtccgcgagc aggccaaggc catcatcggg gccgatctcg tcaagagcga gaagttcacc
    12361 acctcgatga aagacgggat cgacatccgg gagagtctgc gccattggca tcagcggcaa
    12421 aagccgggtc gtccgcaacg gatggagatt tacgtcaaag agattccccc cgcgcgcggg
    12481 aatgtggata cggtgatctt tctgttcgac acccccggcg atccggagca atacagctgg
    12541 caggccacct ggtatgccga acatgcgcag gagtccacgc tctgtttcta tgcgacaccc
    12601 ttcctgaaca acatgatcgc accgggtatc gggcaatcgc gctacggcgg cgccctgttt
    12661 atgttcccgc cccgcccgat ccccgacatc tggaccgacg aagcactggg atttgccagg
    12721 accctcgaag aacgtttgat tgcggccgga gcagtccatt cacgtgaaac gcacgtggcc
    12781 ctggtgacac cggttccacc caaggcgcgt tggaggcaga tcgccagaca attcgaccgg
    12841 cgtctggtgc cgattccgtt gagccggttt tcaggacaga tgatcgaccg tctgcggcgg
    12901 ttccatgtat tgaatggaca cgagatccgc agcttcgcgg cccaatttat tcgtgagtaa
    12961 ccagtccatg cctcatcaga cagccgagca acgaatcgcc gatcttcgtc gtcaaatccg
    13021 ccatcacgat cacctctatt acacgaagga ccggccggag atttccgacg cggaatacga
    13081 ccggctcttt cgcgagctcg tcgacctcga aaccgcccat cccgacctgg tcaccggcga
    13141 ctcgcccacc caacgagtcg gcgcaccacc gctggaggaa ctcaccaagg tttcccacga
    13201 gaaaccgatg ctgagtctcg attcgattac cgatcaggat gatgtgcgcg cctttgatgc
    13261 gcggatgaag cgggagctgg aaaccgacca gatcgtctac acggcggaac cgaaattcga
    13321 tggcctgtcg gtggaactgg tttacgaccg gggcgccttc gttcgtggcg cgactcgtgg
    13381 agacggcatg gtcggcgaag acgtgacgat caatttgcga acgatccgcg cgttgccgct
    13441 acagctcgat ccccagccct ccctgcccgc acacctggtc gtgcgcggcg aagtctacat
    13501 gcgtttggat gagtttcagg tcctgaaccg ccgcatgacg gaacggggcg aggaagcctt
    13561 tgcgaacccg cgcaatgccg cagcaggatc gttgcggcaa ctggacagcc gcatcaccgc
    13621 gtcgcgaccc ttgaccctga cctgttacga catcatggca ctatccggca cagccccacc
    13681 cacccactgg gaggagttgg acgccttggc cgcctggggc cttcctgttc ccgaacaccg
    13741 gcaacgctgc cgctcgatcg aggaagtcct ctcgttccat gccggcaccg atgggatgcg
    13801 ggactctctg ccgtttgaaa tcgacggcat cgtcgtcaaa ctcgaccgcc ggacgtggca
    13861 agaccagctt ggcagcaaat cccgcagccc ccgttgggcc atcgcttaca agtttgcacc
    13921 ccgaaaagaa atcacggtca ttcaggacat cgccgtctcg gtcggtcgca ccggtaccct
    13981 gacgccgctg gccctgctga agccggtgga ggtcggaggt gtcacgatca gccgggccac
    14041 cctccacaat gcggatgagg tcgcgcgaaa agacgtgcgc gtgggggaca ccgtcaaggt
    14101 ggaacgcgcc ggcgacgtga tccccgccat cgccgaacgg gtccccgttc ccggggaaac
    14161 gagacaggct ccgttcgtca tgccggacca ctgcccggtg tgcggatcgg ctgtggcccg
    14221 cgaaggggct tactactact gtaccggcca gaccgtgtgc gtcgcccaac tgaagggcgc
    14281 cattgaacac ttcgcatcca agagcgcgct ggatatggac gggatgggaa agaagacgat
    14341 cgcccaactg gtcgacgtag gattggtccg cgacctggcc gacctctaca gcctcaccaa
    14401 agagcagctg ctcagcctgg aagggttcgc cgaccggtcg gccactctgc tgatggaatc
    14461 catagaacga agcaaatcca cctctctcga ccggctgtta atggggctgg gcatcaggca
    14521 ggtggggcag cacatcgccc gcgtcctggc caaacagttc ggcacgttgg cgaagctgat
    14581 gtcggcgacg caggaggaat tgctgcaggt gcgcgaaatc ggcccggaga tttctaccag
    14641 cctggcctcg ttcttcagcg aagaacggaa ccgggcggtg attgcccgct tgatcgaacg
    14701 gggtctgaca atcgctccac cgtcgtctga aatcgcagcc ggcagccaga gcctggccgg
    14761 caagacgttc gtctttaccg gtgggctggc caactacacc cgcgaccagg ccaaacagtt
    14821 ggtggaacaa caggggggac aggtctcgtc gagtgtcagc aagaagacat cgtttgtggt
    14881 ggcagggacc gatccggggt cgaaactgga ccaggcccgg aaactggggg tgcgggttct
    14941 gacggaaacg gaatttaccg ctctagtgac gcctgagtga ccgagacatg ctcatcgacg
    15001 atgggctcat gcgtgatggg gtgtttttct tccttgtaga tattgacgct cttgatgacc
    15061 cgcgggtccg cctcatgaat gaccaggcga caattggcga tatgcacttc ttcaccgacc
    15121 ttgggaatcc tgcccagttc atgctgcaca agaccgctga tcgtcacggc atcgtcgcct
    15181 aaatccacct tgaggaaatc gttgaccttc cgaacctcgg tgcgtccatg caccaggatc
    15241 tggttcttgc cgatacgctt gatcaactct tcggtgatat ccgtctcatc gacgatctcg
    15301 ccgaccacct cttccagcag gtcttccaac gtgacgagtc ccatcacacc cccgaactcg
    15361 ttgaccacaa tcgccatgtg gcgtttgtcc aactgaaact gcttcatcag gtcgtccgcg
    15421 gatttcgtat gcgggatgaa cagggccggt tgcgcgatgt cgcgaagctt catctccgta
    15481 tgaccctggg ccaacgccgt cagcgccttg gtcttgtaaa gaatcccgat aatgttatcc
    15541 agcgtgcctt cgtataacgg gatacgcgaa tacttggact tgaacaggag ttccttcgct
    15601 tcgcgcaggt actgattgca gtcgagcgag aacatgtaga tacgcggggt catgcagtct
    15661 tcggccgtga tgtccttcag ctggaagacg ttcttgatca tcttcacttc ctgtgcctcg
    15721 atggcgccgc tcttgctgct ctgatccagc atgatcttca gctcttcttc cgtaacgaag
    15781 ggcacgttga gccctttccc accggtcagt ttatagatga gcggcaccac gaagaacatg
    15841 atcggcgtga gcacctgctg ggccgcatag gccggatagg ccatgttcaa caccacaggg
    15901 accgagtact tggcagccag ggtcttgggc acgagatcgg caaacaccag cagaacaaag
    15961 gtcagaatac cgaccaggac cgcgaaggct tcgcccagca caccttccag cccctgccct
    16021 ccgaatcggt tcagcgcaat aatcgtggcc agcgatgcgg tggcggtgtc gatcagtcga
    16081 tctccgacca ggatggtcag caggaggcgt tgcggatcgg ttcgaagatg gagggccatc
    16141 tccgcccgct tgctgcccgt atcggccagc gccctgagct tcgtatcgtt gacggagtag
    16201 aatccgactt caacaacaga aatcaccgcg gacaacagaa tgaggactat cagaacgata
    16261 atgtccataa gacctatcga ggttagaggt gcagactcac gttgagggag tgaatggtgg
    16321 aaccttggat cagtccgaag ccttgggttc gaactgcaag acttgcccta tgtcgtgaga
    16381 agacgaccag accgggtcct ctcgggtcac tttcatccat cgcgtattac aattagcaca
    16441 ggcagacagg cgcatcaagc attacgacac ttccatgccg agcacaatta gtcagcatat
    16501 cagtcattta gactatttac catgctcccg caccctcaag gattccccgt agtcgccccc
    16561 ggcgcatcgt cgcgcacgat gaatggatcg gtctccagtg cccgtctcag atgcgcggcg
    16621 gcctgctcgg ctgcgacctc cgtttgaaat tgtcccgcat acactcgata ccggcgacca
    16681 tccggaagat cgacgccgac gatctttgaa ccaggatagg cactcctcaa ccgttcgacc
    16741 aaagttcgtg cgttggccgg atcggcaaag gaaccgacct gcacgcgcaa cacccccatc
    16801 cccccggctc gtccctcgta actgatcacc cggagttcga tctcatcggt gccgcgcccg
    16861 accatcccta cagcctgggc tccggccaaa gacaggtcca gaattctccc gcgcgcaaac
    16921 ggcccccgat cgttgatccg cacggtcacc tgccggccgg tgctcagaga tcgcacgacc
    16981 gccaccgacc ccagcggcaa cgtccggtgc gccgccgtca atttatgcat gtcgtagact
    17041 tcaccgttcg ccgtgcggtt gccgtggaag ccgggcccat accaggacgc gctgccccgt
    17101 tcgataaacc ccaacggata gccgggcgga taagaaggac gcgccggact ccagccggag
    17161 cagccgctga gccatgtggt gaggagaaac gcgaacaggg ggagaagaag acggctggtg
    17221 cttgtcctct tctcccccgg cataaggcgg attagaattc gtcgagtgaa tgggtgcgca
    17281 gcaccccgag ggctttggtc gtgttcgaaa cggtcagcac cacgatcgcg cgtttgcctt
    17341 cccgcgacgg ggtgcaatag ccgcacttca cattgatgcg agccctggtg agcgaatcgg
    17401 ccacctgttt caaggcgccg ggcttattct ccagactcaa aatcagggcg gtctcttcac
    17461 ggaacttgat cttggatttt ctcagcgcaa tacgagccgt atccacatcc gccacaatca
    17521 ggcgaagctt tccaggaccg gtgacttcgg gcgcagagaa cgccttgatg ttgactcccg
    17581 cagccccgag cacggccgcc acttcagcca gcactccagg cttactctgt ccactcacca
    17641 caaattgcgt cgttgtcggc atgggctcct ctccctctgc tgagtcagtt cgtctcggat
    17701 gttagcgact gtatccatac agccacttca cgctggtgcc gccgatgttt ctgacggtat
    17761 gggacactcc ggccggaatg aggatttcct cgcccggttc gggcctgaca caccggcccc
    17821 cgaactccag ttccaaccgg ccctcgaccg gcatcaccaa ctcgtcggtc gcgtggcggt
    17881 aatcctccca cacttgcccg ggcggatcaa cccaaagatc acaactgaag cccctcgcct
    17941 gccattccct cgcgactgcc ctctgatcga ccatgaccat agttctccgc atcttgctac
    18001 accttagccc aacgaccttc caacttcttc atgatctgcg gcatggtcgt gtactccatg
    18061 tcgtcgaggg gcaaccggtg cggttcaaag ggaccgtgca tccgtaacac atcggcgatc
    18121 cggttcgcat cccggcgcgc ttcatcatac gacggatcgt cgaacatatc ccgcggcccg
    18181 acgagtttgc cgtcagccaa ttgaaatccc aaacaggcga cgcgcggagg cccatcgaag
    18241 cgggtcggcg tcgcctgctt caccgagacc ggcatgagcg gaccgtaatg cgaaccacgc
    18301 atccagccct cgatgatcca gggataccgg aacggatcga gcacttcgcc caccgccgga
    18361 aagttctgct gcgcgcggac tatcatcacg ggatcgtctt tcccgacata cttgcccgca
    18421 atgagcgaaa gccgctccgt ggacgaagcc gccgcaatcg tcccatccgc ccgtgagtac
    18481 acgtacttga tcgtgtactt accgggggaa ccgatgaaca tcaacatgtc gtagagttct
    18541 tcgggacaat cgaacgagat cttcttgtgc tcgtagatat cgtgcacttc gaaccggaat
    18601 ccctggtgca gcgtgggcgc gatgacgaga cccgccgtat tgaagggatc ggcgaacatc
    18661 ttatacagag ggagattcca ggcacccgcc gacgtcttgt ccgccatgaa aatcagcacc
    18721 ggatcggact tccgctccac gaattccatt tcagccacac ccggcccctg cccgcgcacg
    18781 tttccactga aggcatcggc cagcagatcc tgccctgcgc cgtagagatg cagctccttc
    18841 gcgaccgcag tgccggcctc gaatgtatcc cacgcaagcc ggtggatccc ctcatgatcg
    18901 acgccctggc gatgcgtcat gatcaactgc agatcatccc cgcaagccga gacgtgataa
    18961 tcgacaagca acccgctggt cttcgctacc gcaagcttct ccttcgcgca atccatcaag
    19021 gccggatgca tggccgaatg cccgacgaac ccgccgatgt ccgccttgat cacacttaac
    19081 gtgatgctct gtgtgctctc cgctgccatg ttgcacctct gggtgataaa gcctggttga
    19141 tggcctctgc gccgaatctc cagcgtcggc cctggaaggt cctcatctcg gaatgagcaa
    19201 aggcaatgcc tgagtggcta ttagcaagga agaggtgaat ctgaaggtgt tacgggctcc
    19261 gcgtgagatt accgccggtc atacccgtgg agcggcgcta cagcggaagc cgatcagctg
    19321 tagcagaagg cagcaacgat gggatcgttc acccggcaac aacacagagg gggatgcagc
    19381 tgatgctgca tccccctctg cttcaggccc aaacaacgtt ggttggctta gaaaattatg
    19441 agcaaccgct cgtttctccg cagctcatgc acttgaggca ggttccgttc c
//
LOCUS       DAOQRM010000033        19446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1944_length_19446_cov_15.012016, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000033 DAOQRM010000000
VERSION     DAOQRM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19446
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1944_length_19446_cov_15.012016"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PKK81_04695"
     CDS             <1..465
                     /locus_tag="PKK81_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNV32198.1"
                     /translation="LELFGFPLSGHPLELFADRVAGLSHLPARDLAQHVGKHVTLLGW
                     MVTEKIVSTKQGEPMEFVTFEDQTSLYDATFFPGTYRRYCHLLASDQAYLVTGVVEEH
                     FATVTVTVTRFRPLSASDAEDTIVLEEDAAEPYTERHDTLSPDPLRHFHLDL"
     gene            416..1306
                     /locus_tag="PKK81_04700"
     CDS             416..1306
                     /locus_tag="PKK81_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNV32199.1"
                     /translation="MTLSPLIRFGTSTWTYEGWKGQIYTRAYTKTGFTRDCLGEFCQY
                     LYKGEPLFRTVGNDATFYRPPTVNQLIRYLSQIPEDFEMCAKVWEEITIPTYATHARY
                     GIKAGQRNPNFLNADAFIKLVLQPYRDARFGPHTGPFLFEFQRHGLSVQEFCNELDGF
                     FARLPKDFRYAVEIRNAGMLGPQYHEVLRRHDVAHVYNHWTFMPSLAEQHQRMRTFTA
                     PFSVFRLQTPLKMTHEAAKKRAEPYNKIVGELPEMRQDTVRLVRQAVGENRTTYVLVN
                     NRAEGNAPLTIQALVESLRE"
     gene            1586..2863
                     /locus_tag="PKK81_04705"
     CDS             1586..2863
                     /locus_tag="PKK81_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV32200.1"
                     /translation="MSPVATPTTPPLQLITIDRRVEDHHSSCKIPAPGTQAPTKRHTY
                     RMMALAAVHAYIVFHLISWHVFGIELWGKTAMMGVPSLAKGTINAASIMVLLILGSIL
                     IWGRGFCGWACHMRGAIEFADWILRKLKVRRYLALREKNILVNTPHRWLLRIGALFVL
                     LLPVIILIHNVGFTPKVNVMTPPPIADLPGYEGKAFAKAAFFNFEIKPTWNDFLLAFG
                     LAVFIQFTMSVLLNLRYGQGAFCRILCPYATMMVPLMNISPVQKKITRVAQCTGCRDC
                     SNACPQGIDVSREIFHFNGKVVNTECIKCYACIDACDDNVLQDTAAPGVPQTDRLKPY
                     EKRPWQQELIRKDGLLTNARHMQVFEPLGPVADFSSMIVALICGGITSRFGGFWFYPG
                     AIISFIVFRECCLRVQRWRAVERIQTSIVSVKS"
     gene            2924..3103
                     /locus_tag="PKK81_04710"
     CDS             2924..3103
                     /locus_tag="PKK81_04710"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNV32201.1"
                     /translation="MNTVARVRDYSGLVAEFTIYLKEKKSYWLAPIIFVLMALSALAF
                     FLEGSVLAPAIYSVF"
     gene            3406..3681
                     /locus_tag="PKK81_04715"
     CDS             3406..3681
                     /locus_tag="PKK81_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32202.1"
                     /translation="MAKQPSGRGSVKLSAAASAIKKRLKGVKVIDWHEVGTPHPEVVI
                     GTVQTGVANFKANLGTLAKLKELRDLHILINGTPRPDIAQIKFTLRG"
     gene            3691..4758
                     /locus_tag="PKK81_04720"
     CDS             3691..4758
                     /locus_tag="PKK81_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV32203.1"
                     /translation="MQGPAGQYPTLQVHPGRRCNLQCLHCYSDSGPAVSEQLEIDLLE
                     GVVADAAAIGYRVMSVSGGEPLLYPGLAQLLRAAHTSGLVTTVTTNGMLLDQRQLNVL
                     GADCDLVAISLDGIPESHNFMRNSTRAFDDMCARLPALRASGIPFGFIFTLTFHNVHE
                     LEWVADFAVEQGASLLQLHPLEPVGRATCRLGESYPDGEENAAAVCEAGRIRELYEGK
                     LEVQIDLATVPAMLEHPTRVFVREATLCGAQTVADVIAPLVIETSGIVSPLQYGFPRA
                     WSWGNIKERSLPDLAADWLARDYAAFLTLCRLVYEQAVRNDDEPVLNWYEQAYAAAAQ
                     FSPTQVRENLMQTCSSERSSQ"
     gene            complement(4782..5990)
                     /locus_tag="PKK81_04725"
     CDS             complement(4782..5990)
                     /locus_tag="PKK81_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNV32204.1"
                     /translation="MIRRLQVYSPSSAVLSTKWSASFDGRRGDRSCSCGNTCPDCRAR
                     QVTERLPHAWTGATPSLVHEVLASPGEPLEAGLRAQMESRFGHDFGKVRVHRDGKAAD
                     SATAVQARAYTVGHEIVLGSAAAGLGRRGTVSLLGHELSHVVQQERAFLSSGHPGVLQ
                     RSGLEVPNDTQGQASSASNGPENQAAGLSEPDCDTRVADVFLLGAIHCPQRPECCFAQ
                     VHDRNNRNLTGIFRADHQLEGKPDCDYGDQKHHDFWLANQWRILEITTSEMTVMNMCG
                     REETLNVEGIGTVKGESASAVVPGRAPSSTSLLPNIVDHTQGMWGKTHKIHYDDQCNS
                     LHFVPNEPGKEIKVYIWDSAQDSFVNASDPADTRTPGQLERFAGIVLKEYKEGEWQGT
                     NCGDLPRWAL"
     gene            complement(6039..8489)
                     /locus_tag="PKK81_04730"
     CDS             complement(6039..8489)
                     /locus_tag="PKK81_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNV32205.1"
                     /translation="MGRPVGGRDGQPEEGQAASRPPRIEHHIGRCAIFPPAGTHGGKA
                     EPVPYRASMERAFQADLSDVRAFRGASVLLAGAGARAAAWPETLAFVSLNPPPRIVAH
                     EVAHVLQYRKTPSPIAERRGLVSRRDPAEAEAGRAAGQFESDAPIRVQARPSVAPMYY
                     TDEEEPDSKEFLEERPPLFVGEEVGSWGSSWPATLIARERLAVSVPEGQRSHAIVEAI
                     ALREPIVILHEYGRLWLYRLEWEGLSGRYSRFTNAETIFHKGTNERGDYSVSNVLGRP
                     EVEAFVTEDGGVLSPPGAGRLFSHTGGEFEDPLVSKFQNASAFLDGMGRGLEGAGFEA
                     LAERLRRMAALNVVFPAPFAIGAVRGLVDEMLDLVQLLNPQQWTAIEAAARETILLLN
                     DPDGEILAATLGEEFGRNQAAALDGLLQQSLPIFAYEVGKLIGPTIVELLLALVGIEI
                     GPVAMVQKSLAAMKQIPRMAGVVRDLTLVFPNLPNGPTMPSKLIPDEASSSRTITELL
                     SPEGLVSPRQVPRGLPDLSSEESALLARTGNRREFPDALPKDLADQELAIVKRAEKVS
                     ISDGDGKYINEVDLRNGHRWKEQPNGTWCRFSENPTNCTALVPTPGPVSPGTIVAKAA
                     DKESLLERYTAWATNRGADQVEVAILKAISGTGEYPAGTYAVVVGSRNGVVYPGNTPG
                     VRWLTISHVHPGVANEPGYRHPSLADLAAAEHDTVRLEKLVGGVRKWIHFQTPEGSWN
                     SVKYGYDATIERYFVEIAGEDRLTFERLHDVTDDQLREYDRLDEAGLEQDRINLMNQE
                     NSDGSYLKWYAEQFSFMQ"
     gene            complement(8598..8918)
                     /locus_tag="PKK81_04735"
     CDS             complement(8598..8918)
                     /locus_tag="PKK81_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32206.1"
                     /translation="MATLNQILAEQTKIIGEAKSSLEAAFKKPPTQAATIAAREATVA
                     DLKARVANLTETRAAVTRHIDQQVAGYQSEIVALEKLIEEDKKRFGDQPTPPRPVRGK
                     GRGK"
     gene            complement(8945..12262)
                     /locus_tag="PKK81_04740"
     CDS             complement(8945..12262)
                     /locus_tag="PKK81_04740"
                     /inference="COORDINATES: protein motif:HMM:NF039478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HNV32207.1"
                     /translation="MSGDYSRKRFNPEKHYQGVLRQQGRVDLDADWNEYVDLQDRRWR
                     AETIDVVGRSGVPDETPDGFKIAVSGGELTVGQGRMYIDGYVAENHGTAPAFEPTLEE
                     GYGTAALPVKNQPYGGPVTVPAQVRSLVYLDVWRREVTHLQAPDLIEPAVNVDSTTRY
                     QTAWQVRVLANIPPDITCDTPLSDIPGWPTGNRSSSARLTTTTVAVTTEPDPCLVPPT
                     GGYRGLENHLYRVEVHSATTTTAKVKWSRENAHVATSLLEILAGRTTVRVESLGRDDV
                     LRIKTGDWVELTSDQREFAGLPGEMRKVTVDDANQTLTFSPALPAADFPQGVVDAAKH
                     VRVIRWDQSGIVRKPDGSQLINLDLTSDGLIELSAANPSFVLELGVQATLTVLAGGTA
                     HSGDYWCFAARTADADIERLNQAPPLGIHHHHAHLAIIEPNGEIHDCRTVFPPLTELT
                     PGCCTVVVRPGEDIQAAIDSLPDEGGCVCLKVGDHEIQAPIRIEKSHVSLHGETLGAL
                     VVRTDGPELLRIAHPDGLLLEHVTVSGISFELERKGSQGQVMQAMVAMDRCRNTSVER
                     CVLHARALSQIAGMIITRCNDVSVMNCLVDNVQYGLWTAADTTGLTVVGNSFDATNGR
                     KSDGGLVGLLLQDVSFPARIEENRVLGFVFGIVLNNGLFTGVPFSLAAGSRIAGNYIV
                     RLGAQVEAGTLKAFGIDVAADDCVICDNILLYASVEYGGIAVSGRNAVVERNQLRSVL
                     KEAGAVRPIALLLARLGGKGSLGTIGGRVAGNTVLGVQEGIVVIGNEGAEVLDNRVES
                     DGQEVGFGILLAASSRVRLQGNRVTNTAWPIATSGGTANVVVDNRLVRGGGGVTVVFH
                     TSLTCSQNRVEDMRHWGILSLVGFAKCALTENRVLNCGYQQVPSIGIGASLQIGELCV
                     ESCEVMNTGVSPDNTTVSSLSWGIVADLILEARVQSNLVTYANAALLDANQEHRALWM
                     RGWWEQVITLGAGQVIFGFSAQILDNKFLGPGRSALIEIAQQSVSDNLFRRFERLFFS
                     NNFCWHASVAAQGTATVSLAGRSAIVMGNHIKTNVPIPSVDFHGMKDAVYMGNLAQAN
                     PVNFGGIPSPISGFNKP"
     gene            complement(12355..14436)
                     /locus_tag="PKK81_04745"
     CDS             complement(12355..14436)
                     /locus_tag="PKK81_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32208.1"
                     /translation="MNPEADKVYQLLPALYRIRDAEQGGVLRALCEVLAEDIAVLREN
                     LDQLYDDQFIETCADWVAPYIGDLIGYRTLHGVTERTRSARAEVANTIAYRRRKGTAT
                     VLEQLARDVTGWNARVVEFFQWLETSQYMNHIRPANRATPDLRNWEVLERRDTAFNSL
                     AHSVDVRRIETQQGRYNIPNIGLFLWRLDAFSLRRSPALRVDDERFLFSPLGNNFQLF
                     TRPQSETDITHIAEPLNVPEPISRRVLDRYLDRYYGPQLSLFIEADNVTTTLGNVQVC
                     NLSDDGVTWAHLPVSKVSIDPVLGRIAFPPGTPPVNLRVTCQYGFSMPTGGGSYERSK
                     TFALGGGFDAVTQGQSLQTALTAAQAGGIVEIGDSGRYPETLTLTIPAAAKVEVRAAN
                     EHRPTVVLGGDWTISLSPGSELTLNGLLITGGRVRVTAAGGVGARILRLRHCTLVPGL
                     ALTREGEPLFPAESSLVVERAGTQVEIDHCLLGGVALVDSTELSMTNTLLDATAPTRV
                     AFAAPDGLAAGGALTVVNSTVIGKVHTVRLDLASNTIFAAALAAGDAWTHPVLSDQNQ
                     QGCCRFSFVPLNSIVPRRYRCQPALAVDAALLEADQPKGSLTDPEILALTLSTQARVR
                     PAFTARRYGQAAYGQLAGHCPEEISRGADDESEMGVFHDVFAPQREDNLKIRLQEYLR
                     FGLEAGLFHAT"
     gene            complement(14433..>15658)
                     /locus_tag="PKK81_04750"
     CDS             complement(14433..>15658)
                     /locus_tag="PKK81_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HNV32209.1"
                     /translation="AADAGDASVSEIVFIDDAVETAITHDRDWTTLQLATSLAHVYER
                     TTVRVNANVAAATHGESVKEPLGSGDAAVPYQAFTLRQPPLTYVGAETPSGSASTLKV
                     YVNEVLWHEVPFFYGHGPTEHIYITRKDDEGRTTIRFGDGITGARLPTGQNNVRVEYR
                     KGTGLGGLVQAGQLSLLMSRPLGLKGVVNPAAAQGAEDPESRDDARINAPLTVLTLAR
                     AVSLQDYEDFARTFSGIAKAQAVWVWDGRKRSIFLTVAGPGGEVLAEDGSVITKLKEA
                     LRAYGDPFVAFTVKTYRQAFFRLEGTVTIHSDHVSETVMAEVTADLQRRYVFEARAFG
                     QPVALSEAIAAIQSSAGVVAVDIDTFARNDVPTPAIQPRLIADRPAMGADGLVPAAEL
                     LLLDPASLTQLKAMQ"
     assembly_gap    15659..15758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15759..17113)
                     /locus_tag="PKK81_04755"
     CDS             complement(<15759..17113)
                     /locus_tag="PKK81_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32210.1"
                     /translation="MSQSPQNECGCCAGIAAETPALLFNRPGLSAIAYRVGTHQTFRR
                     SLQARLSDGRWPVLRGLRTRDDDDFTIALLDAWAVTGDILTFYQERIANESYLRTATE
                     RLSILEQSRLLGYQLGPGLAAATHLAFTMEEAPGVPELAAQPTMLAIGTKVQSIPGPD
                     EQPHTFETIETIEARPAWNAFRAQLTETQTLAWDMTELWLAGANVTVTVGDLLLIVAP
                     NGSGYDASIRRVTVVESSREADRARVFLATISTSAGSVSASKPGVYVMRSTVSPFGHN
                     APLQPQYSSGVFQGTFSEWALDGAELDSLLTLSSRNDKILDNSFVVIEQDDPDSGSRM
                     WTFGTVTAVTHRSVARYGLAGNGTRLSLSTGWTKNADSKLDLLRTMTVAAQSEEIALA
                     ERPLSYPVYGETLSLEQLVEGLAPGRPLAVSGKRQAIRIRHPRPAPFRGRKAPIGTPP
                     PP"
     gene            complement(17110..>19446)
                     /locus_tag="PKK81_04760"
     CDS             complement(17110..>19446)
                     /locus_tag="PKK81_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HNV32211.1"
                     /translation="PLGALTLTATNIRIEGGERIVGIRVLNAVAGSGPSAGVLTVEVD
                     QAGDFSLYTLRLVTDTLNDAVPPGIDPQLTSIDFSFKVECPSPFDCAPHHRCPEEQGA
                     APVIDYLAKDYASFRRVMLDRMAVLMPQWTERNPADLGVMLVEALAYTADQLSYQQDA
                     VATEAYLGTARRRVSVRRHARLMDYFMSEGCNARAWLQVEVSADVVRVDPANPAIPVG
                     TRFVTRIPGQLTIIADDPRIYERADTIFEAMEPLQSLYADHNELSFYTWSNQRCCVPT
                     GAVSATLAGHHPHLTPGSILLFEEVLGPETGAAADADPARRHTVRLDRIVATTSGGTP
                     LTDPVTAQPVTEITWHDDDALPFPLCLSAATSAGYRDGVSVARGNLLLADHGVTLAEE
                     ALGVVPEPFLSMPRSKEEDRCAPRSPRMIPPRFRPGLTRAPLTHAGPAYDHAKSAWAA
                     MQWALRDVQPAITLTGTKESETTTWTARRDLLNSDAAADEYVVEIENDGGGAIRFGDD
                     VHGRRPESGTDFTARYRVGNGRAGNIGADSLVHIAIGIPEIIRVRNPLPAIGGQEPES
                     LQDVRQRAPVAYRIQERAVTPVDYAEVTERRADVQRAAATFRWTGSWHTVFATVDRGG
                     GAAVTGEFEADIRAHIERYRMAGHDVEIDGPQFVALEIELHVCVAPEHFRSDVERELL
                     DVLSNRDLPDARRGLFHPDNFTFAQPVYLSSIYAAAHQVAGVESVDVRTFQRQGRPES
                     SALDNGRMDIGRLEIARLDNDRNFAEHGRLTISLGGGK"
BASE COUNT         3760 a         6224 c         5536 g         3826 t
ORIGIN      
        1 ctggagctgt tcggctttcc cctgagcgga catccactgg agctcttcgc agacagggtc
       61 gcgggcctgt cgcatctccc ggcacgggac ctggcacaac atgtcgggaa gcacgtgacc
      121 ctcctcggtt ggatggtaac ggagaaaatc gtgtccacga agcaaggcga gccgatggag
      181 tttgtcacct ttgaggatca gaccagcctc tatgatgcca ccttctttcc cggcacctac
      241 cggcgatact gccacctcct cgcctcagat caggcctacc tcgtcaccgg tgtggtggaa
      301 gaacattttg cgaccgtgac ggtcacggtc acacgatttc gccccttatc cgcttccgac
      361 gcggaggata ccatcgtact tgaggaggac gcggcagaac cgtacactga gcggcatgac
      421 actctctccc ctgatccgct tcggcacttc cacctggacc tatgaaggct ggaagggaca
      481 aatctataca cgcgcctata cgaaaaccgg cttcacacgg gactgtctcg gggaattctg
      541 tcagtatctc tacaaaggcg agccactctt tcgcaccgtg gggaatgatg cgacgtttta
      601 ccggcctccc acggtcaacc agctcatccg ttatctaagt cagattccgg aagacttcga
      661 gatgtgcgcc aaggtctggg aagaaatcac catccccacc tatgccacac atgccaggta
      721 cgggatcaaa gccggacagc ggaatccgaa ctttctcaac gcggacgcct tcatcaagct
      781 ggttttacaa ccctatcggg acgcacgatt cggcccgcac acagggccgt tcctattcga
      841 atttcaacgc cacggactgt ccgtccagga attctgcaac gaattagacg ggttcttcgc
      901 acgtctgccg aaagacttcc ggtatgccgt tgaaatccgg aatgcgggga tgctgggccc
      961 tcagtaccac gaggtgctca gacgacatga cgtggcgcat gtgtacaatc actggacctt
     1021 catgccgtcc ctcgccgagc agcatcaacg gatgaggacc tttaccgcac cgttcagtgt
     1081 gtttcgtctc cagacccccc tcaagatgac ccacgaggca gctaaaaaac gtgccgaacc
     1141 gtacaacaaa attgtcggtg aattgcctga gatgcggcag gacaccgtac gacttgttcg
     1201 acaagcagtc ggcgaaaatc gaaccactta tgtactggtc aacaaccggg cggaaggcaa
     1261 tgcgcccctg acgattcagg ccctcgtaga atcactgcgc gagtaagggc accttctgac
     1321 agtgtgatgt tggcccatca cctgcttccc acgcaggcgc cctccaattc gacccggcta
     1381 tccccgctct aagctttccc atcaccacac gcaaccggac catccggtcc aggggttcat
     1441 tcgacgagcc cataccttta tcgcgcccgc ctccgtcttt cgatgcatag cgcagactgc
     1501 tgatcgcgac tattcttcat caagagcgtt tcacaacgga taccaagccg aggtgggaat
     1561 ctcctaccac cttcaggggg tatatatgag tcctgttgcc actccaacca caccgccgct
     1621 ccaactcatc accatcgatc gccgcgtaga agaccatcac agctcctgca aaattcctgc
     1681 tccaggcaca caggcgccga caaaacgcca tacctaccgt atgatggcgt tggccgcggt
     1741 gcatgcctat atcgtgtttc accttatttc atggcatgtc ttcgggatcg aactctgggg
     1801 aaagacggcc atgatgggtg taccgtcctt ggccaaagga accatcaacg cggcctcgat
     1861 catggtgctg ctgatcttgg gctccatcct gatctggggg cgtggattct gtggctgggc
     1921 ctgccacatg cgcggagcga tcgaatttgc cgattggatt cttcgtaagt tgaaggtccg
     1981 ccggtatctc gccctccgag agaagaatat tctggtcaac acccctcacc gctggctcct
     2041 gagaatcggg gccctgttcg ttctcctgct gccggtcatc attctgattc acaacgtcgg
     2101 attcacgccc aaagtcaatg tgatgactcc accgcctatt gcagacttgc cagggtatga
     2161 aggtaaggca ttcgccaagg ccgccttctt caatttcgaa atcaaaccga cgtggaatga
     2221 cttcctgctg gccttcgggt tggcggtctt tattcagttc accatgagcg tcctgttgaa
     2281 tctgcgctac ggccaaggcg cattctgccg aattctctgc ccctatgcca cgatgatggt
     2341 cccgctcatg aacatctccc cggttcaaaa aaagatcaca cgcgtggctc agtgcaccgg
     2401 ctgccgggat tgcagcaacg cctgccccca gggtatcgat gtcagccgcg aaatttttca
     2461 tttcaacggc aaggtggtga acacggaatg catcaagtgc tacgcctgca tcgatgcctg
     2521 tgatgacaac gtgcttcagg atacggcggc gccgggcgtg ccgcaaaccg atcgcttaaa
     2581 gccgtatgaa aaacgcccct ggcagcagga attgattcga aaagacgggc tgcttaccaa
     2641 tgccagacac atgcaggtgt tcgaacctct cggacccgtt gctgatttct catcgatgat
     2701 cgtggcgttg atttgcggag gaatcacgtc ccgcttcgga ggcttctggt tttaccccgg
     2761 agccatcatt tcgttcattg tgttcaggga atgttgtttg cgcgtgcagc gatggcgggc
     2821 tgtggagaga atccagacat ctatcgtatc cgtcaaatcg tgatgtgtcg accggcagtc
     2881 ggttcaagat aggttctcat caatcgttta caaggaggcg catatgaata cagtcgcacg
     2941 agtgagagac tactcaggcc tggttgcaga gtttacgatc tacttgaagg agaaaaagtc
     3001 ttattggctg gcgccgatca ttttcgtctt aatggccctg agtgcgctcg cgttcttcct
     3061 cgagggaagc gtgttagcgc ccgccatcta ctccgtgttc taattcttgt acgcgcgccc
     3121 gcccccagag cgggcgcgcg ccgcttgccc cacgacacag ccagcattcc tcgctgccgc
     3181 attgcctcac atcgcaacac ttgtaagtcc gaaaccttcc gcaggaatca ctgcctgccc
     3241 ggttgcagct gcgtacgcct aactctcacc gggatcacac gtattcatcg tgatcgggcg
     3301 gtgagatgtg cttgtcgttt tatgtaagaa gttgtatggt gcgcccatct agtcacgacg
     3361 tgctcatgaa cgagcgcgac catcacaatc caaaggaggt ctccgatggc aaaacaacca
     3421 agcgggcgcg gatccgtgaa gttaagcgct gcggcaagtg cgatcaagaa acggctgaag
     3481 ggtgtcaagg taatcgattg gcatgaggtc ggcacgccgc atccggaagt cgtgatcggc
     3541 accgtgcaga cgggcgtggc gaacttcaag gccaacctcg gcacgctggc gaaattgaag
     3601 gaactgcgtg atctccatat cctcatcaac ggcacgccgc ggccggacat cgcccaaatc
     3661 aagttcacgc tgcgaggcta agtaccgatc gtgcaaggac cagccggaca gtacccgaca
     3721 ttgcaggtcc atccgggccg acgatgcaac ctgcaatgct tgcattgtta ttccgattcg
     3781 ggaccggcgg tgtctgaaca gttggagatc gatctgctgg agggagtggt cgccgatgcg
     3841 gccgccatcg gatacagggt tatgtcggtg tcgggcggcg agcccttgtt gtatcccggg
     3901 cttgcccaac tccttcgggc ggcccatacc tcggggctcg tgaccaccgt cacgacgaac
     3961 ggcatgcttc tcgatcagcg tcaactgaac gtcttagggg ctgattgcga cctggtcgcg
     4021 atcagcctcg acggcatccc ggaatcccac aacttcatgc ggaactccac gcgggcattc
     4081 gacgacatgt gcgcgcgttt gccggcactc cgggcctccg gcatcccctt cggctttatc
     4141 ttcacgctca cgttccacaa cgtacacgag ttggagtggg tcgcagattt tgccgtcgag
     4201 cagggcgcct cccttctgca gctccatccg cttgaaccgg tcggcagagc cacctgcaga
     4261 ctgggcgaat cctatcctga cggcgaagag aatgcggcgg ccgtctgcga ggccggacgt
     4321 atccgcgagt tgtacgaggg caagctggaa gtgcaaatcg atctcgcaac ggttccggcg
     4381 atgctggagc atccgacgcg cgtcttcgtg agggaagcga cattgtgtgg cgcgcagacc
     4441 gtggccgacg tcattgcccc gctggtgatc gaaaccagcg gcatcgtctc accgttgcag
     4501 tatggattcc cgcgtgcctg gtcctgggga aacatcaagg agcgatcgtt gccggatctt
     4561 gccgcagact ggctcgcccg cgactatgca gccttcctca cgctgtgccg cctcgtctat
     4621 gaacaggcgg tgcgcaacga cgacgagccg gtgttgaatt ggtacgaaca ggcgtatgcc
     4681 gcggccgcgc aattttcccc cacgcaggtg cgcgagaacc tgatgcagac ctgttcatct
     4741 gaaagaagca gtcagtagca ggcgggtcgc atccctccct ctcacagagc ccacctcgga
     4801 agatcaccgc agtttgtccc ttgccactcg ccctctttgt attccttcaa cacgatcccg
     4861 gcgaaccgct cgagttgacc gggagtcctg gtatcggccg gatcactcgc gttcacaaaa
     4921 ctatcctgcg ctgaatccca gatgtagacc ttgatctctt tacccggttc attggggacg
     4981 aagtggaggg agttgcactg gtcatcgtaa tggatcttgt gtgttttgcc ccacataccc
     5041 tgggtatggt cgacgatgtt cggcagcaac gacgtcgatg aaggcgcacg accgggaacg
     5101 acggccgatg ccgactcccc cttgaccgta ccgatgccct ccacattcaa ggtctcctcc
     5161 cgtccgcaca tgttcatcac ggtcatctcc gaggtggtga tctcgagaat tctccattgg
     5221 ttcgccaacc agaagtcgtg gtgtttctgg tcgccatagt cgcagtccgg tttgccttcc
     5281 agttgatggt ctgcacgaaa aatgcccgtg aggtttctat tgttcctatc atgcacctgg
     5341 gcgaaacagc attccggtcg ctggggacag tgaatcgctc cgagcagaaa cacatccgcc
     5401 actcgggtgt cgcaatccgg ttcggataaa ccggcagcct gattctcagg cccattcgac
     5461 gcagacgagg cttgtccctg cgtgtcgttc ggcacctcaa gtccggaacg ttgcagcaca
     5521 ccgggatgcc cagagctcag gaacgccctt tcctgttgca ccacgtggga cagttcgtgg
     5581 ccaaggaggg atacggttcc tcttctcccc aaaccggccg ccgccgagcc caacacaatc
     5641 tcatggccga ccgtgtacgc cctggcctgg acggccgtgg ccgaatccgc ggccttgcca
     5701 tcgcgatgca cacgcacctt cccaaaatca tgcccgaaac gcgactccat ctgcgcgcgc
     5761 agtccggcct ctagcggctc ccccggcgac gcaagcacct catggacgag ggatggggtc
     5821 gcgccagtcc aggcatgcgg aagtctctct gtcacctgtc tggcgcgaca atccggacag
     5881 gtgttgccgc aggagcaaga tcgatcaccc cgacggccat caaacgaggc actccatttc
     5941 gtggacaaaa cagccgaact gggcgagtaa acctgaagtc ttcgtatcat catcgacctc
     6001 ctgtcgccat gcttgcacct tctcttcggt ccggatcgct actgcatgaa gctgaattgt
     6061 tccgcgtacc acttgagata cgagccatcg gagttctctt gattcatgag attgatgcga
     6121 tcttgctcca accctgcctc atcgagtcga tcgtactcac ggagttgatc atctgtgaca
     6181 tcgtgcagtc tctcaaaagt gagtctgtcc tcgcctgcga tctcaacgaa ataccgctcg
     6241 atggtcgcat catacccata cttcactgaa ttccagctcc cttctggcgt ctggaaatga
     6301 atccactttc tgacgcctcc gacaagcttt tcgagccgga ctgtatcatg ctcagccgct
     6361 gcgaggtccg cgagcgaggg atggcgataa ccaggctcat tcgccacacc agggtgcaca
     6421 tgggagatgg tcaaccacct gactcccggt gtatttcccg gatagaccac accgttcctt
     6481 gagccgacca caaccgcata ggtcccggcc ggatactcac ccgttccaga aatcgccttg
     6541 agtatcgcca cctcaacttg atccgcgcca cgattcgtgg cccatgccgt gtaacgttcc
     6601 agcagtgatt ctttatccgc agccttcgcc acgatcgttc ctggtgacac tgggcctggc
     6661 gtcggtacca gcgcggtgca gttggtcggg ttctctgaga accggcacca ggtcccatta
     6721 ggttgttctt tccacctgtg gccgttcctc agatcgacct cgttgatgta cttgccgtca
     6781 ccgtcgctga tcgaaacctt ctccgcacgc ttcacgatgg cgagttcttg atcggccagg
     6841 tccttcggca atgcatcggg aaactccctc cgattcccgg ttctggccaa gagtgccgac
     6901 tcttccgatg acaggtcggg aagtcccctt ggaacctgtc tcggcgacac aagcccctct
     6961 ggagagagca actcagtgat cgttcggctg ctcgacgcct cgtcgggaat cagcttggac
     7021 ggcatcgtcg gaccattggg tagattcgga aacaccagtg tcaggtcacg gactacgccc
     7081 gccatgcgcg ggatctgttt catcgccgcc aacgatttct gcaccatcgc caccggaccg
     7141 atttcgatgc cgaccagcgc gagcagaagt tccacgatcg tcgggccgat caacttgccg
     7201 acctcatagg cgaaaatcgg taggctctgc tgcaggaggc catcgagtgc agccgcctgg
     7261 tttctgccga actcttcccc cagcgtggcg gctaatatct cgccatcggg atcgttcagc
     7321 agcaggatcg tctcccgagc cgccgcctcg atggccgtcc actgttgcgg attcagcaat
     7381 tgcacgaggt cgagcatttc gtccaccagg ccccggacag cgccgatcgc gaagggtgcc
     7441 ggaaacacca cattcagggc ggccatacgg cgcagccgtt ccgccaaggc ctcgaagccc
     7501 gctccttcca ggcccctgcc catgccgtcg agaaaggccg acgcattctg aaacttcgac
     7561 accaacggat cctcgaattc cccgccggtg tgggaaaaca accggcctgc tccgggaggt
     7621 gacagcaccc ctccatcctc cgttacaaag gcttcgactt ccgggcggcc tagaacgttg
     7681 gagaccgaat agtctcctcg ctcgttcgtc cccttgtgaa aaatcgtctc agcattggta
     7741 aaacgggagt agcggccgga gagtccctcc cattcgagcc ggtagagcca gagacggccg
     7801 tactcgtgca gaatcacgat gggctctcgc agtgcaatgg cctccacaat ggcatggctg
     7861 cgttgcccct ccggcacact taccgccagt cgctcgcgcg caatcaacgt cgccggccat
     7921 gaactccccc agcttcccac ctcctcgccc acaaagagcg gcgggcgttc ctccagaaac
     7981 tcctttgaat cgggttcctc ttcgtcggtg taatacatcg gagccacgga cggacgggcc
     8041 tgcacccgaa taggagcatc ggactcgaac tggcctgcgg cgcgaccggc ttcggcttcc
     8101 gccggatcgc gacgggaaac caatccccgc cgttcagcga tgggtgacgg agtcttgcga
     8161 tattgaagga cgtgcgcgac ttcgtgcgcc acgatgcgcg ggggaggatt caatgacacg
     8221 aaggccaatg tttccggcca ggcagccgca cgggcgccgg cccctgccag caaaacggat
     8281 gcgccacgaa aggctcgaac gtcagagagg tcggcctgga aggccctctc catgctggct
     8341 cggtagggaa cgggttctgc ttttcccccg tgagtccctg cgggcggaaa gatcgcacat
     8401 cggccgatat ggtgctcgat ccggggtggg cgggaggcgg cctgtccctc ctcgggttgc
     8461 ccatcacggc ctccgaccgg acggcccatc gacggtcctg tccagcaggg ccccagcatc
     8521 ccctcctgct gaggcgatcc cgctctggcc tgcatgaccc gctccataac cgttccagaa
     8581 agggacggga acagccttta cttccctcgc cccttgccgc gcaccggccg cggaggcgtc
     8641 ggctgatcgc cgaaccgttt tttgtcctct tcgataagtt tctccaaggc cacgatttca
     8701 gactgatatc cggccacctg ttgatcgatg tgtctggtga cggcagccct ggtttccgtg
     8761 agattggcca cccttgcctt caaatctgcc accgtcgctt cccgagcggc gatcgtggcc
     8821 gcctgggtag gtggcttctt gaatgcagcc tccagggacg acttcgcttc accgatgatt
     8881 tttgtctgct ctgccaggat ctgattcaag gtcgccatct ctatgctcct ttacccacgg
     8941 actgctaggg tttgttgaaa ccggatatcg gggatggaat gccgccgaaa ttcaccgggt
     9001 tggcctgggc caagttgccc atatacaccg cgtctttcat cccgtgaaaa tccacggagg
     9061 ggatcggcac attcgtcttg atatggttgc ccatgacgat ggcgctccgt cccgcgagag
     9121 acaccgtagc cgtcccctgc gcagcgacgc tggcatgcca gcaaaaatta ttgctgaaga
     9181 aaagccgttc gaaccgccga aacagattgt cgctcacgga ttgttgggcg atttcgatca
     9241 acgctgaccg gcctggtccg agaaacttgt tatccagaat ctgagcactg aagccgaaaa
     9301 tcacctgccc cgcgccgagg gtgatcacct gttcccacca gccacgcatc cagagcgcgc
     9361 gatgttcctg attcgcatcc aacagcgccg cgttggcata ggtgaccaga ttgctctgca
     9421 cccgggcctc cagaatcagg tctgcgacga ttccccagga cagcgaactc accgtcgtat
     9481 tatcgggtga gactccggtg ttcatcacct cgcacgattc cacgcacaac tctccgattt
     9541 gcaaggaagc cccgatgccg atcgacggca cttgctgata gccgcaattg agcacgcggt
     9601 tttcggtcaa ggcgcatttc gcaaatccga ccaggctaag gatgccccaa tgtcgcatgt
     9661 cttcgacgcg attctggctg caggtcaggg acgtgtggaa gaccaccgtg acgccgccgc
     9721 cgccgcgtac gagacggtta tcgacgacga cattcgccgt gccgccgctg gtcgcgatgg
     9781 gccaggccgt gttggtgaca cgattgcctt gcaggcggac acgactcgat gccgcaagca
     9841 agataccgaa cccgacctcc tgcccgtcac tttccacccg gttgtccagt acctcggccc
     9901 cttcgttgcc gatcacgacg atcccttcct gcacgccgag gaccgtgttg ccggcgaccc
     9961 ggccgccgat cgtcccgaga ctccccttcc cgccaagccg ggcgagcagg agagcaatag
    10021 gccgcacagc ccccgcttcc ttcagtaccg aacggagttg gttgcgctcg acaacggcat
    10081 tcctgccgct caccgcaatc cctccgtact cgacggaggc atacagaaga atattatcgc
    10141 agatgacgca gtcatcggcg gcgacgtcga tcccgaacgc cttcagagta ccggcttcca
    10201 cttgcgcacc gagccgcacg atgtagttac ccgcgatcct cgagcccgca gcgagcgaaa
    10261 acggcacgcc ggtaaacaga ccgttgttca acacgatgcc gaacacaaac cccaacacgc
    10321 gattctcttc gattctcgcc gggaaggaca catcctgcag caacagaccc acgagtccgc
    10381 cgtcgctctt cctcccgttc gtggcatcga agctgttgcc cacaacagtc aggcccgtcg
    10441 tatcggcggc cgtccagagg ccgtactgca cattgtcgac cagacagttc atcacactca
    10501 cgtcgttgca gcgagtgatg atcattccgg cgatctgact caaggcccgc gcatgcagga
    10561 cgcagcgttc gacggatgtg ttccggcagc gatccattgc caccatggcc tgcatcacct
    10621 ggccctgact ccccttcctc tccagttcga aggagatgcc gctcacggtg acatgttcca
    10681 agagcaggcc gtcaggatgg gcgatacgca ggagttcggg gccgtccgtc cgcacgacca
    10741 gcgcgcccaa tgtttcacca tgtaatgaga cgtgtgattt ctcgatgcgg atcggcgcct
    10801 ggatctcgtg atcgccgacc ttgaggcaga cgcagccgcc ctcatcgggc aacgagtcga
    10861 ttgccgcttg aatatcttca ccgggccgca cgaccaccgt gcaacaacca ggcgtcaatt
    10921 ccgtcagcgg cgggaagacc gtccgacagt cgtgaatctc accgttcggt tcgataatcg
    10981 cgaggtgcgc gtggtgatga tgaatgccga gcggcggcgc ttgattgagc cgctcgatgt
    11041 cggcatccgc cgtccgggcc gcgaaacacc aatagtcgcc gctgtgggcc gtgcctcccg
    11101 cgaggaccgt tagggtggct tgtacgccaa gctccaacac aaaacttgga ttcgctgcgg
    11161 acagttcgat caacccgtcg gaggtgaggt ccaggttgat cagttgcgac ccatcaggct
    11221 tccgcacaat gccggactgg tcccaccgaa tcacgcgcac atgcttggcg gcatcgacga
    11281 caccctgcgg aaaatcggcc gccggcagcg caggactaaa ggtcagcgtc tgattggcat
    11341 catccaccgt caccttgcgc atctcgccgg gcagaccggc gaactcgcgc tgatcgctcg
    11401 tcaattccac ccaatctccg gtcttgatcc gcagaacatc gtcgcgcccc aggctctcca
    11461 ctcgcacggt cgtgcgtccg gcaaggattt ccaatagcga ggtggccaca tgggcgtttt
    11521 cccgcgacca tttcaccttc gcggtggtcg tcgtcgcgct gtgcacctcc acacggtaga
    11581 ggtggttctc caggccgcgg tacccgccgg tcggcggcac gagacaggga tccggctccg
    11641 tggtgacggc caccgtcgtg gtggtaagcc ttgccgaaga ggaacggttc ccggtcggcc
    11701 agccgggaat gtcgctgagc ggggtgtcac aggtgatatc cggcggaatg tttgccaaca
    11761 ccctcacttg ccaggccgtc tggtaacgcg tggtcgaatc gacgttgacg gccggctcga
    11821 tcagatccgg cgcctggagg tgggtcactt cccgccgcca cacgtccaga tagactaacg
    11881 accgtacctg agccggcacc gtgaccggtc cgccataggg ttgattcttt accggcaagg
    11941 ctgcggtccc atacccctct tcgagagtcg gctcgaatgc cggggcggtc ccgtggttct
    12001 ccgccacata cccgtcgata tacatacggc cctggccgac cgtgagttcc ccgccgctca
    12061 ccgcgatctt gaacccgtcg ggcgtctcat cgggcacacc ggagcggccc acgacatcga
    12121 tcgtttcagc ccgccaccgg cggtcctgga ggtctacgta ctcgttccag tcggcatcga
    12181 ggtcgacccg cccctgctgc cgcagtacgc cctggtaatg tttctcaggg ttaaatcgtt
    12241 tacggctgta gtcaccactc atgtgcgctc tcctgctgat cagtgcggtt tacgtcaatg
    12301 gttcggacac cggtgggaag actggttccc acatccttgc tcaaagtctt ttcgtcacgt
    12361 ggcatggaag agtcccgcct ccagcccgaa cctgagatac tcctgcaagc gaatcttgag
    12421 attgtcttca cgctggggag cgaagacatc gtggaacaca cccatctcgg actcatcgtc
    12481 cgccccgcgg ctgatttcct caggacaatg accggccaac tgaccataag ccgcttgccc
    12541 ataccgccgc gccgtgaagg ccggcctcac gcgagcctgc gtcgacaagg tcagcgcgag
    12601 tatttccgga tcggtgaggc tgcctttggg ctgatcggct tccagcaacg ccgcatccac
    12661 ggcgagagcc ggctgacaac gataccggcg cggcacgatg gaattcagcg gcacgaacga
    12721 aaatcgacag cagccctgct ggttctgatc ggacagaacc ggatgggtcc aggcatcgcc
    12781 cgccgccagc gccgcggcga agatcgtgtt ggaggcaaga tcgagacgca ccgtatggac
    12841 tttgccgatc accgtggaat taacaacggt caaggccccg ccggcagcca ggccatcagg
    12901 cgctgcaaag gccactctgg tcggcgccgt cgcatcgagc aaggtattcg tcatggagag
    12961 ttccgtgctg tccaccaagg ccacaccccc gagaagacaa tgatcgatct cgacctgcgt
    13021 gccggcccgc tcgacgacca gagaagactc agcggggaac aagggctcgc cttcacgcgt
    13081 gagggcaagt cccggcacca gcgtgcagtg gcgcaaccgc aggatacgcg ctccgacgcc
    13141 accggctgcc gtcacccgca cgcgtcctcc ggtaatgagc agcccgttca gcgtgagttc
    13201 cgaaccgggc gacagactga tcgtccaatc gccgccgagg acgacggtcg gtcgatgttc
    13261 atttgcggcg cgcacctcca ccttggcggc cgccgggatc gtcagggtca gggtttccgg
    13321 ataccgtcca ctgtcgccga tctccacaat cccgccggcc tgcgctgcgg tgagtgcggt
    13381 ttgtaaactc tgcccctgag tcaccgcgtc gaacccgccg ccaagcgcga aggtcttgct
    13441 gcgctcgtag gacccgcctc cggtcggcat actgaacccg tactgacagg tcacccgcag
    13501 gttgaccggc ggagtgccgg ggggaaacgc gattcgtccg agcaccggat cgatcgacac
    13561 cttactcacc ggcaggtgcg cccaggttac gccgtcgtcg gacaggttgc acacctgaac
    13621 attgccgagc gtcgtcgtga cgttgtccgc ttcgatgaac aggctcaact gcggcccgta
    13681 ataccgatcg agataccgat ccaacaccct ccgactgatc ggttccggca cattgagggg
    13741 ttcggcaatg tgggtgatgt cggtctccga ttgcggtcgc gtgaacagtt ggaagttgtt
    13801 ccccaacgga ctgaacagaa accgctcgtc atcgacgcgc aaggccggcg agcgccgcag
    13861 cgaaaaggcg tccagccgcc agaggaacaa tccgatgttg ggaatgttat accgcccctg
    13921 ttgagtttca atccgccgca cgtccacgga atgcgccagg ctgttgaagg ccgtatcgcg
    13981 ccgctccagc acctcccagt tccgcaggtc cggcgtggcc cgattggccg gacggatgtg
    14041 gttcatgtac tggctggtct caagccattg gaagaactcc acgacgcgcg cgttccaccc
    14101 cgtcacgtct cgcgccaatt gttcaagtac cgtcgccgtt cccttgcgac gccgataggc
    14161 aatcgtattg gccacctcgg cgcgcgcgct gcgggttcgc tccgtcacac cgtgcaacgt
    14221 ccggtagccg atcaggtctc cgatgtaggg agccacccaa tcggcgcagg tctcgataaa
    14281 ctggtcatcg tagagttgat cgagattctc tcgcagcacc gcaatgtctt cagccagcac
    14341 ctcgcacaat gcgcgcaaga cgccaccttg ttcggcatca cgaatgcgat acagcgccgg
    14401 aagaagctga tagaccttgt ctgcttctgg attcattgca tagccttcag ttgagtcagt
    14461 gatgcaggat cgagcaacaa caattccgcc gccgggacca ggccatccgc gcccatggcc
    14521 gggcggtcgg cgatcagacg aggttgaatg gcgggtgtcg gcacgtcatt gcgggcgaac
    14581 gtatcgatat cgaccgcgac cacacccgcg ctggactgta tcgccgcaat cgcctcactc
    14641 aacgccaccg gttggccgaa cgccctcgcc tcgaagacgt accgtcgttg caaatcggcc
    14701 gtcacctcag ccatcacggt ctcgctcacg tggtcggagt gaatcgtaac cgtcccctcc
    14761 agccgaaaga atgcctgccg atatgtttta accgtgaagg cgacaaaggg atcgccataa
    14821 gcgcggagcg cttctttgag cttcgtgatg acggacccgt cttcggcaag gacctccccg
    14881 cccggcccgg cgacggtgag gaaaatactg cgtttccgtc catcccacac ccacacggct
    14941 tgggccttgg cgatgccgga gaaggtcctg gcgaagtcct catagtcctg gagcgaaacc
    15001 gcacgcgcca gcgtcaagac agtgagcggg gcgttgattc gggcgtcgtc tctcgactcc
    15061 gggtcctcgg ccccttgcgc cgcggcagga ttcacgaccc ccttcaaccc cagcggacgg
    15121 ctcatcaaca gactcaattg accggcctgc accagcccgc cgagtcccgt gcccttccgg
    15181 tactccacgc gcacgttgtt ctggccggtg ggcagccgcg cccccgtgat gccgtcaccg
    15241 aagcgtatcg tcgtgcgccc ctcgtcatcc ttcctcgtga tgtagatgtg ttccgtagga
    15301 ccatgcccat agaagaacgg cacctcatgc cacagaacct cgttcacata gaccttgagc
    15361 gtggaggccg atccactcgg cgtttccgcc cccacgtagg tcaagggcgg ttgccgtaat
    15421 gtgaacgcct gatagggcac ggctgcatca ccgcttccca agggctcctt cacgctttcg
    15481 ccatgtgtgg cggcggcgac gttggcgttc acgcgtacgg tcgtccgctc gtagacatgc
    15541 gccaaggagg tcgccaactg cagggtcgtc cagtcgcgat cgtgggtgat ggccgtctcg
    15601 acggcgtcgt cgatgaagac gatctctgaa accgaggcgt cgccggcgtc ggctgcgann
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg aggcggcggc gtaccgatcg
    15781 gtgccttccg tcccctgaac ggcgcagggc ggggatgcct gatacggatc gcctgacgct
    15841 tcccgctcac cgcgagggga cgccccggcg cgagtccctc gacgagctgc tccagcgaca
    15901 gggtctcgcc atagaccgga taggacagcg ggcgctccgc caacgcgatc tcttcgctct
    15961 gggccgccac cgtcatggtg cgcaacaggt ccaacttgga atcggcgttt ttcgtccaac
    16021 cggtactcag gctgagtctc gtcccattgc cggcgagacc ataacgggcc accgagcgat
    16081 gggtgacagc cgtcaccgtg ccgaacgtcc acatgcgcga gccactgtcc ggatcgtcct
    16141 gttcaatgac gacaaaactg ttgtcgagaa tcttgtcgtt ccggctgctg agggtgagga
    16201 gactgtccag ttcggcgcca tccagcgccc actcgctgaa cgtcccctga aacaccccgc
    16261 tgctgtactg aggctgcaac ggcgcgttgt gcccgaacgg cgagaccgtg ctccgcatga
    16321 catagacacc gggcttacta gcggatacgc tccctgcact ggtcgagatc gtggcgagga
    16381 agacccgcgc gcggtctgct tcgcggctgg actccacgac ggtcactcgt cgaatcgacg
    16441 cgtcgtagcc gctgccgttc ggggcgacga tcagcaacag gtcgccgacc gtcaccgtca
    16501 cgttcgcgcc ggcgagccac agctccgtca tgtcccaggc gagtgtctgc gtctcagtca
    16561 gctgcgcacg aaacgcgttc catgctggtc tcgcctcgat ggtctcgatg gtctcaaagg
    16621 tgtgcggctg ctcatcgggc cccggaatgc tttgcacctt cgttccgatc gccagcatcg
    16681 tgggctgagc cgccagctcc ggtacgcccg gcgcctcctc catggtgaag gcgaggtgcg
    16741 tcgctgcggc cagtcctggc ccgagctgat acccgagcaa ccgggattgt tccaggatgg
    16801 acagccgttc cgtcgcggtg cggagatagg actcgttggc aattcgttcc tgatagaacg
    16861 tcaggatgtc gccggtcacc gcccaggcgt ccagcagcgc gatcgtgaag tcgtcgtcat
    16921 cgcgtgtgcg caatccacgt aacaccggcc aacggccgtc ggagagcctg gcctgcaggc
    16981 tccggcgaaa cgtctgatgg gttccgactc gataggcgat cgctgagagg cccggcctgt
    17041 tgaagagcag ggcgggagtt tccgcggcga tcccggcaca gcaaccgcat tcgttttgtg
    17101 gcgattgact cattttccgc ctcccaggct gatggtcagt cggccgtgct ccgcaaaatt
    17161 gcggtcgttg tccaggcgag cgatttccag ccgaccgata tccatgcggc cattgtccag
    17221 cgccgaactc tccggtcggc cctgccgttg aaacgtgcgg acgtccaccg actcgacccc
    17281 tgccacctga tgggccgccg catagatcga actgagatag accggttgcg cgaaggtaaa
    17341 gttgtcggga tggaagagtc cgcggcgggc gtccggtaga tcccgattgc tcagcacatc
    17401 gagcagttcg cgctcgacat cgctccggaa atgctccggc gccacacaga catgcagctc
    17461 gatctccagt gccacgaact gcggtccatc gatttccaca tcgtggcctg ccatgcgata
    17521 ccgctcgatg tgcgcgcgga tgtccgcctc gaattcaccg gtcactgctg cgccgcctcc
    17581 gcgatcgacc gtggcgaaga cggtgtgcca actgccggtc caacgaaacg tcgcggcggc
    17641 ccgttggaca tcggctcggc gttcggtcac ctcggcataa tccaccggtg tcacggcccg
    17701 ctcctggatg cgataggcca ccggggctcg ctgacgcaca tcctgcaagg attccggttc
    17761 ctgcccgcca atggcgggca agggattccg cacccggatg atttccggaa tgccgatcgc
    17821 aatatggacc aatgagtccg ccccgatatt gccggcgcga ccgttgccga cccgatagcg
    17881 ggcggtgaag tcggtgccgg attccggccg ccggccatgc acatcgtcac cgaagcgaat
    17941 cgctccacca ccgtcgttct cgatctcaac cacatactcg tcggccgccg cgtcactatt
    18001 cagcaggtct cgtcgtgcgg tccacgtcgt cgtctcgctc tctttggtcc ccgtcagcgt
    18061 tatcgccggt tgcacatcgc gcagggccca ctgcatagcg gcccacgcag actttgcgtg
    18121 atcgtaagcg ggccccgcat gtgtcagggg agccctggtc aagcctggtc tgaaacgggg
    18181 tggaatcatc cggggagaac ggggcgcaca acggtcttcc tccttcgagc gcggcatgga
    18241 caggaacggc tccgggacca cacccagcgc ttcttcggcc agcgtcactc catggtcggc
    18301 cagcagcaga ttaccgcgag ctacgctgac accgtctcga tacccggcgc tggtcgcggc
    18361 tgagagacag aggggaaacg gcagcgcatc gtcgtcatgc cacgtgatct ccgtgacggg
    18421 ttgagcggtc acaggatcgg tcagcggcgt cccgccgctt gtcgttgcga caatacgatc
    18481 gagcctcacc gtatgacgcc gcgctggatc cgcatcggct gccgctcccg tttccggacc
    18541 gaggacttct tcgaacaaca ggatgctccc cggcgtcagg tgaggatgat gacccgcgag
    18601 cgtggccgag accgcacctg tgggcacgca acaacgttga ttgctccagg tataaaacga
    18661 cagttcattg tgatcggcat acagagactg gagcggctcc atggcttcaa agatcgtatc
    18721 ggcgcgttca tagatacggg gatcgtcggc gatgatcgtg agttgcccgg gaatgcgggt
    18781 gacgaatctg gtgccgaccg ggatcgcagg attggcggga tcgacccgca ccacatccgc
    18841 cgacacctct acctgtagcc aggccctggc gttacagccc tcgctcatga agtaatccat
    18901 gagacgggcg tggcggcgga ccgagacgcg tcggcgggcc gtccccagat aggcctcagt
    18961 ggcgaccgca tcctgctggt aactcagctg atcggcggta taggccagcg cctccaccaa
    19021 catgacgccg agatcggccg ggttgcgttc ggtccattgc ggcatgagca cggccatgcg
    19081 gtccagcatg acacgcctga aactggcata gtccttcgcc aggtagtcga tgaccggcgc
    19141 agccccctgc tcctccggac aacgatggtg cggcgcacaa tcgaaggggc tgggacattc
    19201 gactttgaac gaaaagtcga tagacgtcag ttgcggatcg atgccgggag gcaccgcgtc
    19261 gttgagcgta tcggtcacca gacgcagggt atacagggag aaatcgccgg cctgatcgac
    19321 ctccacggtc aacacgccgg cggacgggcc gctcccggcg accgcattga gcacgcggat
    19381 accgacgatg cgctctccgc cttcaatccg gatgttggtc gccgtcagag tcaacgcgcc
    19441 caacgg
//
LOCUS       DAOQRM010000034        19329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_1966_length_19329_cov_13.774982, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000034 DAOQRM010000000
VERSION     DAOQRM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19329
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1966_length_19329_cov_13.774982"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKK81_04765"
     CDS             complement(<1..188)
                     /locus_tag="PKK81_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNV32212.1"
                     /translation="MTGYQRHIFVCTNKREPDDARGSCSKLGSDALHACFKQEAKRLN
                     LKGVVRANKAGCLDYCAQG"
     gene            complement(227..595)
                     /locus_tag="PKK81_04770"
     CDS             complement(227..595)
                     /locus_tag="PKK81_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32213.1"
                     /translation="MDAGSCNACSAAGAPLMKFSLGKDFFGRTYDRLSPASDQSPKWY
                     CEPCSMMKNLQRDFRDIRAEFDKLAKGQASALSEPEAKQRAQLRLNEIAAIAGTQAAG
                     SSLLSSTDVKQLIEQFHARV"
     gene            758..1861
                     /gene="ald"
                     /locus_tag="PKK81_04775"
     CDS             758..1861
                     /gene="ald"
                     /locus_tag="PKK81_04775"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNV32214.1"
                     /translation="MIIGVPKEIKDYEYRVSLTPDGARVLLQAGHRVVVEPSAGQGSG
                     FSDDAYRQAGVQVAGSKAEVFQQADLIVKVKEPQLSECALFRPGQVLFTYLHLASLPD
                     LTKALVAADITAIAYETVEARDHSLPMLRPMSEIAGRLAVQIGAHYLGTVQGGRGLLL
                     AGVPGVPPAHVVVLGAGVVGTSAVRIAVGMGARVTVINLDLDRLRFLDDLYGGRIATC
                     AATESAIERAVVEADLVIGAVLVPGARAPKVVSRGLVKKMKPGSVIVDVAVDQGGCCE
                     TTRPTTHSDPVYVVDGVLHYCVTNMPGIVPHTSTLALTNTTLPYIVRLASEGVEKAIR
                     SDPGLAKGVNVMNGRITCQGVADAHGLRFTPVM"
     gene            1897..1973
                     /locus_tag="PKK81_04780"
     tRNA            1897..1973
                     /locus_tag="PKK81_04780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1931..1933,aa:Pro,seq:cgg)
     gene            2287..2634
                     /locus_tag="PKK81_04785"
     CDS             2287..2634
                     /locus_tag="PKK81_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNV32215.1"
                     /translation="MPGVVLALRLVFDQWEGCAAMTDTGESVRARMLVSGRVQGVGYR
                     AFTCRMAVARGLTGHVENLDSGQVAVDAEGNRGVLEEFLSDLKKGPVGSRVTQVQVEW
                     SRATGRSQDFTIK"
     gene            2685..3647
                     /locus_tag="PKK81_04790"
     CDS             2685..3647
                     /locus_tag="PKK81_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV32216.1"
                     /translation="MNSPVSARRPRVARGDEDEDAESGSHAEAEESGADNRPAESGRA
                     EGLDTIKSYLREVRKSTLLNFKQEQALGKRVMEGDEAARQEMIEANLRLVISIGKRYM
                     NRGFPFADIVEEGNLGLIKAVEKFNYKRGFRFSTYASWWIRQFIERAIINQGKLVRLP
                     VHVVERLNRYLGKVEQLVHELGREPRADEVAAKLKTSVAEVDDLKQLVRTTCSLDSPI
                     SDRQDTFLRDVIEDPLCLTPAETTEGVMRRAELMAWVKELPEKERTVILARFGLDGAE
                     SRTLEEIGQEMGLTRERVRQIETAALARLRGIIERKTMKRADLL"
     gene            3672..4187
                     /locus_tag="PKK81_04795"
     CDS             3672..4187
                     /locus_tag="PKK81_04795"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNV32217.1"
                     /translation="MNYKALIREVPDFPKPGILFYDITTLLKNAQAFRSIADELAARY
                     EGQRIAKIIGIESRGFIMGGTLAARLGAGFVPVRKPGKLPADIYEVEYNLEYGSSVLA
                     IHRDAVSPGERVLIVDDLLATGGTAAATIDLVRQLGGEIAGLDFLIELKGLNGRDRLA
                     GYDVHSMIIYP"
     gene            4293..4868
                     /locus_tag="PKK81_04800"
     CDS             4293..4868
                     /locus_tag="PKK81_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNV32218.1"
                     /translation="MATKVHAKKKTPTTKAKAPAPEKPVKKERPAPVTAMTEKDEDSV
                     AARVVAALTLKETPKEREERQRRREVLQRMLLSKRQEIMREIEGNLGQSLTEDQQRRL
                     ESARDVGDQALMDLDRELGISLMEMRNRKRQAIDEALTRLSEGTYGICAECGIEVSEK
                     RLEAVPFAKLCVQCQSRQELLEKIEKEEDRD"
     gene            4989..>5644
                     /gene="queC"
                     /locus_tag="PKK81_04805"
     CDS             4989..>5644
                     /gene="queC"
                     /locus_tag="PKK81_04805"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNV32219.1"
                     /translation="MTHVDSKAVILLSGGLDSTVTAAIAKQDGYRLYCLTISYGQRHR
                     VEVERARAVAQALSAAAHVVVDVDLRAFGGSALTDDVSVPKDRTQEERAGGVPVTYVP
                     ARNTIFLSLALAYAETLEAQRIYFGANVLDYSGYPDCRPDFIRAFEVVARLGTKMGIE
                     GRAVEVCAPLLMLSKAEIVQRGQELRAPLELTHSCYDPGADGVACGRCDSCRIRREGF
                     R"
     assembly_gap    5645..5744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5939..6115
                     /locus_tag="PKK81_04810"
     CDS             5939..6115
                     /locus_tag="PKK81_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32220.1"
                     /translation="MTPEEFFMYLIIALVIIAPIGARLYRRLTLNRTTQMLRDQLKQS
                     QGPRTTTPPSEPPR"
     gene            6142..6534
                     /locus_tag="PKK81_04815"
     CDS             6142..6534
                     /locus_tag="PKK81_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV32221.1"
                     /translation="MKVWVMGVGLAVMVAALAACESNATNQDAAKPAGGVTPAEMQVG
                     EAKFTANCAACHGVRGVGTKQGPPLVHKIYEPNHHADMAFQRAAENGVRAHHWEFGNM
                     PKIEGVTSGDVEHIIRYVRWLQREAGIY"
     gene            6724..7551
                     /locus_tag="PKK81_04820"
     CDS             6724..7551
                     /locus_tag="PKK81_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV32222.1"
                     /translation="MRHGAWSMLGAVGVLLLTSGCVAIEAGHEGVMVEQPFFFGHGGV
                     DPAPSKTGRVWVAPTTKVIEVDVRPLQYSEHFDIISAENAPVSFDAFMIANVVEGRSP
                     EIVGRYGTNWYQNNVKEAFRTFVREEVQKYPLFQLTTDPTTRTKLQDAIAREVQTKLI
                     DKQNLPIRLNRVVVGSILPPKGVVEQTTQTIVQEQRKITMVEFQKAEEAREKAEKQRG
                     IADRAYRESLGLTAPEFVDLRRIEVQKEIVQHSPSALTVIMGLERVGINMPPLASDR"
     gene            complement(7637..8368)
                     /locus_tag="PKK81_04825"
     CDS             complement(7637..8368)
                     /locus_tag="PKK81_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32223.1"
                     /translation="MTPQLSHSGSGTLLAIAAQCLVFSATAQALPEHSRVDVTGDYRY
                     AYHEPETASEARQHACTEALYQAISTSAAVREQTASIVDSKLFRNLVHTLASKHVTDQ
                     QIIQQSETGRTVYCKVKAVFHADEVERVLIAQTTGGTEPGLDQNRALKIVSAREEADG
                     TLVIVYQALKRLDWLGTAYQGSLRESADVMVDFYDDQGVLVRSSRHPARKTATGDDVM
                     NPGEVGTLRIAKPINAKTYRVWVVK"
     gene            8667..10931
                     /gene="malQ"
                     /locus_tag="PKK81_04830"
     CDS             8667..10931
                     /gene="malQ"
                     /locus_tag="PKK81_04830"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNV32224.1"
                     /translation="MYDGTEQDLLRLLASRAGISPEYHDIAGTLHVTSDETRRAILSA
                     MGFQVGSGAVLSQELTALDEAPWQQPCDPILILQQGYGPTQWTFRPALEEGEEQHVAV
                     AWSLTDEKGTIVHREETSPGLNPCEVRSLAGRRMVRVELPLVQNLGLGYYDLSVGTMG
                     SSKVVTRGTLRVVVAPPHCFVPEELARGGRVWGLAVQLYSLRSQTNWGVGDFTDLSRL
                     VEWAGKELGAGIIGLNPLHALKNSRPHHLSPYSPTSRLFLNELYIDLDRLPEYHASPD
                     AQRLRNSAEFQKELEQARAADLVDSESVIKAKRMMLDLAYRQFLKDNYTGIEPSLEPT
                     TARGWLLHRFIRDEGESLERFALFQVLEEERRLIEQSPKLWPEWPAQYRAPGSPALRE
                     FSRRHRKRVRFFQYMQWVAADQLRAANTRAAQVHMTVGLYPDLALGSDRNGAEAWMLQ
                     DVLALGADCGAPPDAFAPQGQNWGFAPFHPLRLKSTGYRSFIELLRKTLRQGGAIRID
                     HVMTLFRLFWVPRGMSAEAGAYVQYPAEDLLRILALESLRAQTLVIGEDLGTVPDYVR
                     EQLARYKILSYRVFYFERNWDGSCKSPSAYPEQSLAVVTTHDLPTLTGYWTGEDIRLR
                     ARLGMYANEQAVQQAFEERTRDRGHILRVLNGAGLLPSGVSDQPDSVPVMTSALCRAI
                     HVYLATSPAWVVMANLDDVIGEVTQMNLPGTVDAYPNWSRKLSLSLEELQRDERVQAL
                     AAAVCTLRPPAATA"
     gene            10962..12113
                     /locus_tag="PKK81_04835"
     CDS             10962..12113
                     /locus_tag="PKK81_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32225.1"
                     /translation="MKSRNHVVLVIAAILFLIVMVMEWMFPLNVVGAFGFVLPILLVA
                     TVRNRRLMIVTLALCILVTFVGLFQPGKKRERFTAVVFNRIMVACVLTGVAYIGVTWE
                     ERKAREEAAKAALATQTEHLLLANTQLVEIKDKLARSERLAAVGQLVASVAHEVGTPL
                     HSIAWHVEALAEEPGVTPAMQKRIGIIDEQLNRVVRIIQDLLSSTRQRKPEPTWYPAE
                     RLIEPVVALMEPGYHAKGVTLHTEVAPALVVWADAEKIHQVLVNLLSNALAATGAGGQ
                     VTVAVTIRQASSEEMESASCTGRSNLSTVVTLVVSDSGCGMPREDVERAFQPFFTTKA
                     VGKGTGLGLFLSREAVSAHGGRLTLESEIGKGTTVTVVLPGLPSDSAAV"
     gene            12135..13529
                     /locus_tag="PKK81_04840"
     CDS             12135..13529
                     /locus_tag="PKK81_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV32226.1"
                     /translation="MSQAKLFVVDDDEAARTLLAEALVKEGYEVEAFSSGQAAVERGK
                     QSRADVVLTDIRMEQGDGFLVLKEFKKFSPDTSIVLLTAFGSLEGAIEAIKQGAYDYL
                     AKPFKRDEIRLVVQRSLEHCRLVRENARFREDARAREPWSQLVGSSPAMLEVYKLVAR
                     VSEGRSTVLIEGESGTGKELIARAVHLNSPRRDKPFIPVNCGALPDHLLESEMFGYEK
                     GAFTGAVGAKAGLFEAADSGTLFLDEIGDLGQALQVKLLRVMQEQEVRRVGSTASVKV
                     DVRIIAATNRDLATLVKEGKFRDDLYYRLNVVRIVLPSLAERREDITMLAHHFLQKYA
                     KQSLHVRGFLPETMALLKRYHWPGNVRELENAVERAVSLSHGPLLLPEDLPESIRAEP
                     DSGELLKGQPAEGDQDALLTLDEVEKRHLARVLKETRGNKVKAAKILGIDRRTLYRMA
                     ERFGLDFGDEAEEK"
     gene            complement(13537..13890)
                     /locus_tag="PKK81_04845"
     CDS             complement(13537..13890)
                     /locus_tag="PKK81_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32227.1"
                     /translation="MEVILPVPAEQVKTALSDVLTQGGYTVDQDDTGNITTGMRQEIR
                     SPWNGLLRWRFGVGKTRVEARVVPQDDSTTRLRLQVFHRGKDGIFDSWEDAETPLPQS
                     AANEIRLLKNALRLL"
     gene            complement(13980..14234)
                     /locus_tag="PKK81_04850"
     CDS             complement(13980..14234)
                     /locus_tag="PKK81_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNV32228.1"
                     /translation="MPDRAYVLINVHPGQTADVVKALGDIKEIKQIDPCWGKPDIFTI
                     VEIPHQDALTQLVLAKIHAIPGVDQTDTHMVYRLQEGKPK"
     gene            complement(14361..14792)
                     /locus_tag="PKK81_04855"
     CDS             complement(14361..14792)
                     /locus_tag="PKK81_04855"
                     /inference="COORDINATES: protein motif:HMM:NF037406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32229.1"
                     /translation="MCARFHLVARQLRLRKEYRPTLEITDEYDLQDLFYALLRLQFDE
                     VGTEEWAPPYADGARRTSYLLDWEKTVVVVKQTRSGLTSRDIAEQIATDKAHYSGRPN
                     GATLLCFIYDPDGRVGNPRGLEADLSTVGDTYRVEIIVAPK"
     gene            complement(16181..16675)
                     /locus_tag="PKK81_04860"
     CDS             complement(16181..16675)
                     /locus_tag="PKK81_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV32230.1"
                     /translation="MAPGQKQPSTILIVDDDPSALLVSTKPLRDAGYTVLQAPGSAEA
                     LKLYAEHPSPIHLVIADVFLPPPGFQLSVDRNPYPRVNGLDMVERLLQDKREIRVLLV
                     SSSPSSELQNRGLVRAGLPFLKKPFTSETLLSLVREVLAGPPAAPDQKNATRPGNGDV
                     EWVD"
     gene            complement(16713..17516)
                     /locus_tag="PKK81_04865"
     CDS             complement(16713..17516)
                     /locus_tag="PKK81_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32231.1"
                     /translation="MTSTTHAAENRPFWTEQALFHFGEDVFFTGRASCAPNIEDGRQR
                     AYVAAVQEVKNFTRTQEVGGFPIDTQMIFEDLHPADCAQGLITVWRLLRVPRTALESL
                     SRHSSPGKQKDISPIQAQITAIRNLTPRVGMLRDEIWNRYGLPRSIWARPEQGELIWD
                     YSQFGLIIVFNQDDVLTRWRLNGPHPRSSEDEPILSRQDNPTGKDLPAIDLTVRLQQL
                     EEQQDLDRRRQAQRYCTIRFADVPESLRPKHGGCEQREYERLKEQHPPF"
     gene            complement(17720..18409)
                     /locus_tag="PKK81_04870"
     CDS             complement(17720..18409)
                     /locus_tag="PKK81_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32232.1"
                     /translation="MKPANLFICLSLLFLATAGCHGYAFIETPTTDGFHSKLPSPYTR
                     AVVWGGRPDTVQSASTWLLKKGVLVVDQTKVLQAAADQKVSLTGYQYLETDVLRMAKL
                     VGARLVVFSNAEVGSWEALDWSSGFPHSQRVYSATVALRAVDVNTGEIEWSGKAQSTE
                     RFSNIEEGVSLLTCHALATAWGLRNPGAVAPESICPPGSGLMSTEMSPPKAHAAPASS
                     GRENLTPSMNY"
     gene            complement(18720..18851)
                     /locus_tag="PKK81_04875"
     CDS             complement(18720..18851)
                     /locus_tag="PKK81_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32233.1"
                     /translation="MIEFPLILIGYALLPLIAIQLMIMADRPQKTGEQNAFPQVEIL"
BASE COUNT         3744 a         5156 c         6289 g         4040 t
ORIGIN      
        1 ccctgtgcgc aataatcgag acagccggct ttgttggcac ggacgacgcc tttcaggttg
       61 agtcgttttg cctcctgctt gaagcaggcg tggagcgcat cggagccgag cttggagcag
      121 ctgccgcgcg cgtcatccgg ttcgcgcttg ttggtgcaga caaaaatgtg tcgctgatag
      181 ccggtcatgt cagcaagtcc gaggttccag ggtgcgatgt gagggattag acccgggcat
      241 gaaattgctc gatcaactgc ttcacgtccg tcgagctgag gagcgatgag ccggcggcct
      301 gggtgcctgc gatggcggca atttcattga ggcgcaactg cgcccgttgt ttggcttcag
      361 gttccgacag ggcggaggcc tgccccttgg cgagtttgtc gaattcagcg cggatatctc
      421 ggaaatcacg ttgcagattc ttcatcatgg agcagggttc acaataccat ttcggacttt
      481 gatccgaagc gggcgacagc cggtcatagg tccgcccgaa gaaatctttt cccagcgaaa
      541 acttcatcaa tggtgcgccc gcggcgctgc aggcattaca ggatccggcg tccatgcgtg
      601 aggtgctcct cttaagcgga aatggtcgat agtctgcgaa tcttaccgca ctgatctttc
      661 gcgtagcaag gtgcgcaggt tcctgacggt agtcacgctc ggccatgtat aatacggggt
      721 atcagggcga agcggtgtgg cgtgcaggga ggcggctatg atcatcgggg ttccgaagga
      781 aattaaggac tacgagtatc gtgtgagtct gacgcctgac ggcgctcgtg tgttgctgca
      841 ggcggggcat cgggttgtgg tagagccgtc ggcggggcag gggagcggat tttctgacga
      901 cgcgtatcga caggccggcg ttcaggtggc cggctccaaa gccgaggtgt ttcagcaggc
      961 ggacctgatc gtgaaggtga aggagccgca gctgtccgag tgcgcactct ttcgacccgg
     1021 gcaggtgctg tttacctatt tgcatctggc gtcgttgccg gatttgacca aggcgttggt
     1081 cgcggccgat atcacggcca ttgcctacga gaccgtcgag gccagagatc acagcttgcc
     1141 gatgttgcgg ccgatgagtg agattgccgg gcgtctggct gtgcagatcg gggcccatta
     1201 tctcgggacg gtacagggag ggcgcggtct cctgctggcc ggcgtgcctg gggtaccgcc
     1261 tgctcatgtc gtggtgttgg gcgccggcgt ggtgggaacg tcggccgtca gaatcgccgt
     1321 gggcatggga gcgcgggtga ccgttatcaa tctggatctt gatcggctcc ggtttctcga
     1381 cgatctgtac ggcggacgaa tcgcgacatg cgcggcgaca gagtcggcta tcgaacgcgc
     1441 ggtcgtcgaa gccgatctgg ttatcggagc ggtgttagtg cccggggcgc gcgcgccgaa
     1501 ggtcgtttcg cgaggactcg tgaagaaaat gaagccgggt tccgtgattg tggatgtggc
     1561 ggtcgatcag gggggctgtt gtgaaaccac caggccgacc acgcactcgg atccggtcta
     1621 tgtggtggat ggcgtcttgc attactgcgt cacgaacatg cccggcatcg tgccccatac
     1681 ctcaactcta gccttgacca acacgacgct cccttatata gtccggctcg catcggaggg
     1741 agtcgagaaa gcgattcgat cagaccccgg tcttgccaag ggtgtgaacg tgatgaacgg
     1801 cagaattacc tgccaaggtg tggccgatgc gcacggcttg cgttttaccc ccgtgatgta
     1861 agacaatccg gcttcccgtt ttcggttctg cccggtcggg gcgtagcgca gcccggtagc
     1921 gcactgcgtt cgggacgcag gggtcggagg ttcaaatcct ctcgccccga ccaaaccgca
     1981 cttcgtgcga ccccgcacct tgaatatgtg cgggatatct gtcctcccgg caccatccag
     2041 acacagcctg cttgattcgg agctggttct cctttaccgg caagggtggg catcctacgt
     2101 cagcgctcgt ctctaggtgt ctttcttcac tgtgactctc ctgctcctgc cacgtatcgg
     2161 attggcctct tgcgcccccg ctcatgcttc gccaggtacc tgccgataag gtaggcgtat
     2221 gagtctggtg atgccgcgta aacttgcaaa tcgcgtgacg ctctctggga gctgtgagag
     2281 tatccaatgc caggcgtggt actcgctctg cgcttggtgt tcgaccagtg ggagggctgc
     2341 gcggcgatga ccgacaccgg cgaatcagtg cgcgcgagaa tgcttgtcag cgggcgtgtg
     2401 cagggggttg ggtaccgggc ctttacctgt cggatggcgg ttgcgcgtgg attgacgggt
     2461 cacgtcgaga atctcgatag cgggcaggtg gcggtagatg ctgaaggaaa ccggggcgtc
     2521 cttgaggagt tcctgtcgga tctcaagaag ggccccgtcg ggtcgcgcgt gacacaggtg
     2581 caggtggagt ggagtcgcgc cacgggccga tctcaggact ttacgattaa gtagtaggta
     2641 acggaattat ggctcgacgt gcagcagtgc gacaggacaa cgcggtgaat tcacccgtct
     2701 cggctcgccg cccacgcgtg gcgcgggggg atgaggacga ggatgcggag tcggggagtc
     2761 acgccgaggc cgaagagtcc ggagcggaca atcgtccggc ggaatccgga cgggccgagg
     2821 gcctcgacac gattaagagt tatctgcgcg aggtacgcaa gtcgaccctg ctcaatttta
     2881 aacaggaaca ggcattgggc aagcgggtca tggagggaga cgaagcggcg cgccaggaga
     2941 tgatcgaggc gaatctccgc ctggtgatca gcatcggcaa gcggtacatg aaccgggggt
     3001 ttccctttgc ggatatcgtc gaagagggca atcttggcct gatcaaagcg gtagagaagt
     3061 tcaattacaa gcgggggttc cgcttcagca cctacgcctc ctggtggatt cgtcagttta
     3121 tcgaacgggc catcatcaat cagggaaagc tcgtccggtt gccggtccat gtcgtcgaac
     3181 ggttgaaccg gtatctgggc aaggttgagc agctggtgca tgagctggga cgggaaccaa
     3241 gggccgacga agtcgccgcc aagctgaaaa ccagcgttgc ggaagtcgat gacttgaagc
     3301 agctggtccg caccacttgc tccctcgaca gcccgatcag cgaccggcag gatacgtttc
     3361 tgcgcgatgt gatcgaagat ccgctctgcc tgacgccggc ggagacgacc gaaggcgtga
     3421 tgcggcgggc tgaactcatg gcctgggtga aagagctgcc tgaaaaggaa cgcaccgtga
     3481 tcttggcgcg gtttggactt gacggggccg agtcgcggac gttagaagag atcggccaag
     3541 agatggggct gacccgtgag cgggttcgcc agattgagac ggctgcactg gccaggctcc
     3601 gcggcattat tgaacggaaa accatgaagc gggcggatct cttatgagaa cgtcccgatt
     3661 cgcttgcacg tatgaattac aaagccctca ttcgagaagt tccagacttt cctaagcccg
     3721 gtatcctctt ctacgacatt acgacccttc tcaagaatgc gcaggccttc cgctccatcg
     3781 cggatgagct tgccgctcgt tacgagggac agcgtatcgc aaagatcatc gggatcgagt
     3841 cgcgaggctt catcatgggt gggacgttgg ctgcccgtct cggtgccggg ttcgttccgg
     3901 tgcgcaagcc ggggaagctg cccgccgata tttatgaggt cgaatacaac ctggagtacg
     3961 gatccagcgt gttggcgatt catcgggacg ccgtgtcacc cggggagcga gtgttgatcg
     4021 tggatgacct gctggccaca ggcggtacgg cagcggccac gatcgacttg gtgcggcaac
     4081 tcggcgggga gattgctggg ctggacttcc tgatcgagct gaaaggcctc aacggacgtg
     4141 atcggctggc gggttacgac gtgcattcga tgatcatcta tccgtaaatc cccaagatct
     4201 tggggagcca ggtacttgtc aaacgatctg tagcgtgata tacctgccga actttttcgc
     4261 cttgcactca tcacgaaagg gcatgagact ctatggcgac gaaagtccac gcgaagaaaa
     4321 aaacaccaac gacgaaagca aaagcccccg ctcctgagaa gcctgtgaaa aaagagcggc
     4381 cggctccggt tactgctatg actgaaaaag acgaagacag tgttgccgcg cgtgttgtgg
     4441 cagcgcttac gctgaaagaa acacccaagg aacgagaaga gcgtcagcgc cggcgtgaag
     4501 tcctgcagcg aatgctcctg agcaaacgcc aggaaatcat gcgcgagatc gaggggaacc
     4561 tcggccaatc gttgaccgaa gatcagcagc gccgcttgga atcggccagg gacgtggggg
     4621 atcaggccct catggacttg gatcgcgaac tcggcatttc gttgatggaa atgcggaacc
     4681 ggaagcgcca ggccatcgat gaggcgttga ctcgactcag cgagggcaca tacgggattt
     4741 gcgcggaatg cggcatcgag gtcagcgaaa agcggttgga ggcggtgccg ttcgccaagc
     4801 tctgtgtcca atgccagtct cgacaggaat tgctcgagaa aatcgagaag gaagaggatc
     4861 gcgactagct cacaggagct agccgcatcc atttcaccgt ccctcttcag cttgtgacgc
     4921 gcctcagccc gtgactggcg gtcgagtttc caaacctcgg tagtctgccc ggccctctcc
     4981 tgttccctat gactcacgtc gattcgaagg cggtgatcct gctcagtggt gggttggact
     5041 ctaccgtcac ggctgccatt gccaagcagg atggctaccg gctctattgt ctgactatct
     5101 cctacggcca gcgccatcgt gtcgaagtcg agcgggcgag ggccgttgcc caggccttga
     5161 gtgccgccgc tcatgtggtg gtggacgtgg atctgcgggc gttcggagga tccgctctca
     5221 cggacgatgt gagcgtgccc aaggaccgca cccaagagga acgggcgggc ggggttcccg
     5281 tcacctacgt gccggctcgc aacaccattt ttttgtcttt ggctctggcg tacgccgaaa
     5341 cgcttgaggc acagagaatt tacttcgggg ccaatgtgct ggactattcc ggttatccgg
     5401 actgccgacc ggattttatc cgcgcatttg aagtcgtcgc caggcttggc actaagatgg
     5461 gcattgaggg gcgggctgtc gaggtgtgtg cgcctctgct gatgctgtca aaagccgaaa
     5521 tcgtgcaacg tggacaggag ctgcgggccc ccttggaatt gacgcatagt tgctatgacc
     5581 ccggcgctga tggcgtggcc tgcggccggt gcgacagttg ccggattcgg cgtgaaggct
     5641 tccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatggcg tggcctgcgg
     5761 ccggtgcgac agttgccgga ttcggcgtga aggcttccgc ctggcgggag ttgtggatcc
     5821 tgtcccctat gcgatactct aacggccatc gaggcgactg aggtgacggt cgcgcggtga
     5881 cgtgagcccg gttcgtattt ccgcctgcga tcacacaaga atctggagag ctgtgcctat
     5941 gacgcctgaa gaattcttta tgtacttgat catcgccctc gtgatcattg cgccgatcgg
     6001 tgcgcggctc taccgccggt tgaccttgaa tcgcacgacg caaatgcttc gggatcagtt
     6061 gaagcaatca cagggacccc ggacgacaac cccgccttct gagcccccgc gatgaaacga
     6121 atcagagtca ggagtattca gatgaaggtc tgggtgatgg gagtcggact cgcggtgatg
     6181 gttgcagctc tggcggcttg cgagtccaat gcgaccaacc aagacgccgc aaagccggct
     6241 ggaggtgtga caccggcgga aatgcaggtc ggcgaggcga aattcactgc caattgtgcc
     6301 gcctgtcatg gtgtgcgcgg tgtcgggacc aagcaaggtc cgccgctggt gcataagatt
     6361 tatgaaccga atcatcacgc cgacatggcg tttcagcggg ccgcagagaa cggcgtccgc
     6421 gctcaccact gggagttcgg caacatgccg aagatcgaag gcgtgacgtc cggagacgtg
     6481 gagcatatca ttcgatatgt gcgatggttg cagcgggagg ccgggattta ttaaggtcac
     6541 ggccggttcg tatccccccg tcggctgtct gttcaccgtc gaccacttgc cctcaacacc
     6601 ccttcgtttg acaatctctg cgtgcggttc gtaaggtatt tgcatgccat catgaggccg
     6661 ttgatgcccg cagtacgtcg ccaggcgggc ctcggtggtg tgaccttttc acaggaggaa
     6721 cctatgagac acggagcctg gtccatgctg ggggctgtgg gggtattgct gctgacgtcg
     6781 ggttgcgtgg ccatcgaggc cggccatgag ggggtgatgg tcgaacaacc gttcttcttc
     6841 ggccacggag gcgttgatcc ggctccctcc aagacgggcc gggtctgggt tgcgccgacg
     6901 acgaaggtga tcgaagtgga tgtccggcca ttacagtatt cggaacattt cgacattatt
     6961 tcggctgaaa acgctccggt ctccttcgat gcgtttatga tcgccaatgt ggtcgaaggg
     7021 cgctcgccgg agatcgtcgg tcgctacggc accaactggt accaaaacaa cgtgaaggaa
     7081 gccttccgca cgttcgttcg ggaagaagtg caaaagtacc cgctgtttca gttgacgacc
     7141 gatccgacga ccaggacgaa actgcaggat gccattgcgc gtgaagtgca gaccaaactc
     7201 atcgacaagc aaaacctgcc gatccggctc aatcgagtcg tcgtcggtag cattctgccg
     7261 ccgaagggcg tcgtcgagca gacgactcaa accattgttc aggagcagcg caagatcacg
     7321 atggtcgagt tccaaaaggc ggaagaggcg cgtgaaaaag ccgaaaagca gcgcgggatc
     7381 gccgatcgcg cctatcggga atcgttggga ctgacggcgc cggaattcgt cgatctgcgc
     7441 cgtatcgaag tgcagaagga aatcgtgcag cactcgccgt cggccttgac ggtcatcatg
     7501 ggattggaac gtgtcggcat taacatgccg cccctggcga gcgatcggta agactcacgg
     7561 atgcaaatgt gttgggagta tcgggacggg gacaggagcg gaagacatcc ggtcctgtcc
     7621 ccggagcctt ggtggattac ttcaccaccc agacacggta ggttttcgca ttgatcggtt
     7681 tcgcgattcg cagagtgccc acctcgcctg gattcatgac atcgtcaccg gtcgcggttt
     7741 tcctggccgg atgacgactg ctccgtacca ggacgccttg atcgtcatag aagtccacca
     7801 tcacatccgc tgattcgcgc aagctgcctt ggtaggcggt tcccagccaa tcgagacgct
     7861 tgagcgcctg gtacacgatg acgagtgtgc catcagcctc ttcgcgcgcg ctgacgatct
     7921 tcagcgcgcg attttgatcc aagccgggtt cggttccgcc cgttgtctgt gcgatcagca
     7981 cccgttccac ctcatctgcg tggaagacgg ccttcacctt acagtacacc gtgcgtcccg
     8041 tttcgctctg ctgaatgatc tgttgatcgg tcacatgctt cgaggcgagg gtatgcacca
     8101 gattgcggaa taatttcgag tcgacaatcg aggcggtctg ctctcgtacc gccgctgagg
     8161 tgctgattgc ctgatagagc gcttcggtgc aggcatgttg ccttgcttct gatgcggttt
     8221 ccggctcgtg ataggcgtac cgataatccc cggtgacatc gacccgcgag tgttcgggca
     8281 aggcctgggc cgttgcggaa aacacgagac attgagcggc gattgcgagc aaggtaccgc
     8341 ttcctgaatg cgacagctgc ggggtcatac gagccacctc cctttccgtg cccggttcca
     8401 cctgatgagc aaagacaatc gtcgcctctc gacgctaagg ttatgctacc acgcgtgagg
     8461 gggcgcagca atgcgacgcc gcgcggcact cgtgcccgga acggaaccgc tatcaatcag
     8521 ccatgagcct cgtcgcgtga agggtaatgc gtcggatcga tgctcagtcg cgaactcgac
     8581 cggcgtgagt catgtcgcaa ctctgcggaa aagcatagga taccttgact tgatggaggt
     8641 cttcactcac actcgtgcac gacactatgt atgatggcac cgagcaagac ctgcttcgtc
     8701 tcctcgcctc acgggccggt atttcacccg aatatcacga catcgccggt acgctccacg
     8761 tgaccagtga cgaaacgcgt cgagccattc tgtccgccat gggttttcaa gtcgggtcgg
     8821 gcgcggtact gtcacaggaa ctgaccgcct tggacgaggc gccctggcaa caaccctgcg
     8881 atccgattct cattctccag caagggtatg gccccacgca gtggacattc cgccccgcgc
     8941 tggaggaagg ggaggagcaa cacgtcgcgg tagcctggag tctcacggat gaaaaaggga
     9001 ccatcgttca tcgcgaagaa accagccccg gcctgaatcc atgcgaggtc cgttcgctgg
     9061 ccgggcggcg catggtgcgc gttgaactgc cgttggttca gaacctggga ctcggctatt
     9121 acgatttgtc ggtcggcacc atgggcagca gcaaggtggt gaccaggggt acgctccgcg
     9181 tggtcgtggc cccgccgcat tgttttgtgc cggaggagct ggcccggggt ggaagagtgt
     9241 ggggcttggc ggtgcagctc tattcgctac ggtcgcagac caattggggc gtgggcgatt
     9301 ttacggattt gtcccggttg gtcgaatggg caggcaagga gctgggcgcc ggcatcatcg
     9361 ggctgaatcc attgcatgcg ctgaaaaatt cgcgtcccca ccatctcagc ccttattccc
     9421 cgaccagccg gctatttttg aacgagctct atatcgacct tgatcggctc ccggagtatc
     9481 acgcgtcgcc ggatgcgcaa cggctgcgga acagtgcaga gtttcaaaag gagctggagc
     9541 aggcacgcgc cgccgatctg gtggattccg aatccgtaat caaggcgaag cgcatgatgc
     9601 tggatctcgc ctaccgtcag tttctgaagg ataactatac ggggatcgaa ccgtcgctgg
     9661 agccgaccac cgcgcgtggt tggttgttgc atcgatttat ccgggacgaa ggggagtcgc
     9721 tggagcggtt tgcgttattt caggtgctgg aagaggaacg gcgtctgatc gaacagtcgc
     9781 ccaagctttg gcccgagtgg ccggcacagt accgggctcc cggttcgccg gcgctgcgtg
     9841 aattttccag acggcatcgt aaacgggtcc ggttttttca atatatgcag tgggtggcgg
     9901 cggatcagtt gcgagcggct aacacccggg cagctcaggt gcacatgacg gtcggcttgt
     9961 atcccgatct tgcactgggg agcgatcgca acggggcgga ggcgtggatg ctgcaggacg
    10021 tgcttgcgct cggtgcggac tgcggcgcgc ccccggacgc gtttgcgccg caagggcaaa
    10081 attggggatt cgctccgttt catccgctgc gcctcaagtc cacgggctat cgctcgttca
    10141 ttgagttgct caggaaaacg ctccggcaag gcggggcgat ccgcatcgac catgtcatga
    10201 cgctgttccg gctgttctgg gtgccgcgcg ggatgagcgc agaggcggga gcctatgtgc
    10261 agtatccggc cgaagacctg ctgcggattc tcgcgctgga aagtctgcga gcgcagaccc
    10321 tggtgatcgg agaggatctg gggacggtgc cggactatgt gcgggaacaa ctggcgcggt
    10381 ataagattct ctcctaccga gtcttttatt tcgaacggaa ctgggatggg tcctgcaagt
    10441 ctccgtcggc ctatcccgag caatcgctcg cggtcgtcac gacacacgac cttccgacgc
    10501 tgaccggtta ttggacggga gaagatatcc gattgcgggc caggctcggc atgtatgcga
    10561 acgaacaggc cgtacagcaa gcattcgagg agcgcacgcg cgaccggggg catatccttc
    10621 gcgtcttaaa cggggcgggg ttgttgccct ccggggtgag cgatcagcct gattctgttc
    10681 cggtgatgac gtccgctctg tgccgtgcga ttcatgtcta tcttgcgacg tcgccggcct
    10741 gggtcgtcat ggccaacctt gacgatgtga tcggtgaagt gacacaaatg aatctcccgg
    10801 gcaccgtgga cgcctatccg aactggtcgc gcaaactgtc gctgtctctg gaggagctcc
    10861 aacgggatga acgcgtacaa gcactcgccg cagccgtgtg tactctccgc cctccggccg
    10921 caactgccta aactgtcctt cctgcgcggc acgacatcgt gatgaagagc cggaatcacg
    10981 tggtcctggt catcgccgca atcctcttcc tcatcgtcat ggtgatggaa tggatgttcc
    11041 cgctgaatgt cgtcggcgca ttcggtttcg tgctcccgat cttactggtg gccaccgtca
    11101 gaaaccggcg gctcatgatc gtgacgctgg cgctgtgcat cctggtgaca tttgtgggac
    11161 tcttccagcc gggtaagaag cgggaacggt tcaccgccgt cgtgtttaat cgcattatgg
    11221 tggcctgtgt gttgaccggg gtggcctata tcggcgtgac atgggaggag cggaaagccc
    11281 gcgaagaagc cgccaaggcc gcgctggcga cgcaaaccga acacctgttg ctggcgaata
    11341 cgcagctggt cgagattaaa gacaagttgg cgcgatccga acggctggct gccgtgggcc
    11401 agttggtggc gtcggtggcg catgaggtcg gcacaccgtt acattcgatt gcctggcatg
    11461 tggaagcgtt ggcggaggag cccggtgtca cccctgcgat gcagaaacga atcggtatta
    11521 tcgatgagca gttgaatcgc gtggtgcgga ttattcaaga tctcttatcg tccacccggc
    11581 aacgcaagcc tgagccgaca tggtatccgg cggaacgcct gatcgagccg gtcgtagcgc
    11641 taatggaacc gggataccat gccaagggtg tgacgcttca cacagaagtg gcgccggctc
    11701 tggtggtgtg ggccgatgcc gaaaagatcc accaagtgtt ggtcaatctc ctgagcaatg
    11761 cactcgcggc caccggggcg gggggccagg tcacggtcgc cgtgaccatt cgtcaggctt
    11821 cctcggagga gatggagagc gcgtcttgca caggccggtc caatctcagt acggtggtca
    11881 cactggtggt gagtgattca ggctgtggaa tgcctcggga ggatgtcgag cgagccttcc
    11941 aaccattctt cacgacgaag gcggtgggta agggcaccgg gttaggctta tttctgagcc
    12001 gggaggctgt gtctgctcac ggcggccggt tgacactcga gagtgagatc ggcaagggca
    12061 cgacggtgac tgtggtgctg cctggtttgc cgtccgactc ggctgcggtt tagcggtata
    12121 ggagagggag agtcatgagt caggcgaagt tattcgtagt ggatgacgac gaggccgccc
    12181 gaaccttgtt ggcggaagcg ctggtgaagg agggctatga ggttgaggcc ttctccagcg
    12241 gccaggctgc ggtggagcgc ggcaaacaat cgcgggccga tgtcgtcctg accgatatcc
    12301 ggatggaaca aggcgacgga tttctcgtgt tgaaggagtt caagaagttc agcccagaca
    12361 cctcgatcgt attgctgacc gcgttcggat ctctggaggg cgcgatcgaa gcgatcaagc
    12421 aaggggccta cgactacttg gcaaagccgt ttaagaggga cgagatccgt ctcgtcgtcc
    12481 agcgcagtct ggagcattgc cgcctggtgc gggaaaacgc ccgttttcgt gaagatgcgc
    12541 gcgcacgcga accctggtcg cagttggtcg gcagcagccc ggcgatgctg gaggtgtaca
    12601 agctggtggc ccgtgtttcc gaggggcgga gcacggtgct catcgaagga gaaagtggaa
    12661 ccgggaaaga actgattgcg cgggctgtcc atttgaacag tccgcgtcgc gacaaaccgt
    12721 tcattccagt caattgcggc gccttgcccg atcatctcct ggagtcggaa atgttcggct
    12781 atgagaaggg ggcgtttacg ggagccgtcg gagccaaagc cgggctgttc gaggccgccg
    12841 atagcggcac gctgtttctc gatgagatcg gcgatctcgg ccaggcgctt caggtcaagc
    12901 tcctgcgggt gatgcaggag caggaggtgc ggcgtgtggg cagtacggca tcggtgaaag
    12961 tcgacgtgcg tatcattgcc gcgaccaatc gggacttagc cacgctcgtg aaggaaggaa
    13021 agtttcgcga cgacttatac tatcgtctca atgtggtgcg gattgtcctg ccgtcgctgg
    13081 ccgagcggcg cgaggatatc acgatgctcg cgcatcactt tctgcaaaaa tacgccaagc
    13141 agtcgttaca tgtgagggga tttttgccgg aaacgatggc gctcttgaag cgctatcact
    13201 ggccggggaa tgtgcgcgaa ctggagaatg cggtggaacg ggcggtctcg ctcagtcatg
    13261 gtccgctgct gttgcccgaa gatcttcccg aatcgatcag ggctgagccg gattcgggtg
    13321 agctgctcaa ggggcaaccc gccgaggggg atcaagacgc gctgttgacc ttagatgagg
    13381 ttgagaagcg gcatctggcg cgtgtactca aagaaacgcg gggtaacaag gtcaaggcgg
    13441 cgaagattct ggggattgat cggcggacgt tatatcgaat ggcggagcga tttggattgg
    13501 atttcggcga tgaggccgag gagaagtagg atgaccctac aatagtcgaa gcgcgttctt
    13561 gagcaggcga atttcgttcg cggcactttg cggcagcggt gtttctgcat cctcccacga
    13621 atcgaatatc ccgtctttcc cgcgatggaa cacttgcagt cgtaatcgtg tggtgctgtc
    13681 gtcctgcggg acgacccgcg cctcgacccg agtcttccct acgccgaatc tccagcgcaa
    13741 tagtccgttc caagggcttc tgatctcctg ccgcattccg gtcgtgatgt tgccggtgtc
    13801 atcttggtcg accgtatagc ccccttgggt caggacatcg gacagtgccg ttttcacctg
    13861 ctcggcagga accggaagta tcacctccat cacatcgggc tccggggcgg gtgggccacc
    13921 cgcacatccg gcgagcgccg agacgagcac gaacgccgca atgccagaga cacgagttct
    13981 cactttggct tgccctcctg aaggcgatag accatgtgcg tatctgtctg atcgacaccg
    14041 gggatggcgt gaattttcgc gagcacgagt tgggtcagtg cgtcctggtg gggaatttca
    14101 acgatggtga agatgtcggg ctttccccaa caggggtcga tttgtttgat ttccttaata
    14161 tccccaaggg ctttgacgac atcagcggtt tggccgggat gcacgttgat caatacgtag
    14221 gctcggtccg gcatggagta aatctcccgt gaggtggtgg ggcgtcaacg cctgccaacg
    14281 aatcgaaacc tagcggattg ctccggatga agtcaacaga acgccctcgg ctcgggcagg
    14341 atagccggcg tcgcgcggtg ttacttggga gccacgatga tttctactct gtacgtgtcg
    14401 ccgactgtcg aaagatctgc ctccaaccct cgggggttgc ccactcgccc gtcaggatcg
    14461 tagatgaagc acaacaacgt ggctccgttc ggacgcccgg agtaatgggc tttgtctgtg
    14521 gcgatctgct cggcgatgtc tctgctcgtc aaccctgagc gagtctgttt gaccaccacc
    14581 accgtttttt cccagtcgag gagatagctc gtccggcgag caccgtcggc ataaggcggg
    14641 gcccattctt ccgtgcccac ctcatcgaat tgtaaccgga gaagtgcgta aaacaggtcc
    14701 tgcagatcgt actcgtccgt gatctccagt gtaggccggt attcttttcg cagccgcagt
    14761 tgtcgagcga ccaggtggaa gcgcgcacac attcggcgga gtgtatcgag cgttgcgccc
    14821 tccgattgat gattgttcct attcgtatcc agcggcgttg tgagtaccgt tggtgtctct
    14881 ggcggtttga cgggagatat gaccggcggt tccggtttgg actcgagaac tgatgcgagc
    14941 gcgggcatgg ctggtgggag cgatatctcg gttcgaactt ggggaacggg cggtcgagga
    15001 tcgcgcgctt gtttttcggc ggctatctcg gttgtggctt ggcttgccgg aatcaccgcg
    15061 acgggggctg gagccggttt ttgtgccggg atgtccgttg gcttggaggc ctgctgcggt
    15121 gtcggagggg gctcgatagg agcagtcggc acaggtgccg ggacggagtt cattcgtgga
    15181 atggggggaa tggttcccga tggaatcgtg gaccgcgccg gcgccattgt cggtagtggt
    15241 gtcggggcgg cctcgccgcg cactgtctgc tccgagggcg ttgcgctatt cgttgatggc
    15301 gcagcgacaa caggttctgc gggcagtgat ggtgaagcgg gcagggatgc cggcgcagtt
    15361 ttctcagccg ggatcccggg ggcttcagat gtggcggagt gcagcggggg tgatggtggt
    15421 tgcgggggca ggcgttgtat cgatgtatcg cgaatcggtt gtgaggcggt cccctgggtg
    15481 accggcgggg aggcggaagt agacgagacg ggtgttgtca taacgatcgg agcgatgggc
    15541 tgagcgggtt ggctgatgct tgtcgtcgct actgagacgg ttgccggagc gttgatcata
    15601 ctgacggttg cgaggggggc ggtgctcgga gcctggggtg gcacgactgg aatagcgggt
    15661 tgagtcggag gaactgccgt cagtgtgggg cgttcagggg ctgtcggtgg aggtggcgcg
    15721 gtttctgccg gtgtgaggcc caaaagctct gccttctgat tctccagctg gacgatcaat
    15781 tctttgagca gagtggtgct ctgtgccgac tccgtgcgat tgccgattcg gagcttgtga
    15841 ttcttgtggg cctggtattc cggcgacttt tcaccgaata gtcgccggat cgattcccgg
    15901 agggaaagtt cggttctggc gcggactgcg tcacgatacg ggaatccatc tctgctcagg
    15961 tcatcgatct gtgagctcag ttcacgcaac gtggtgatgc cacggttgag ttcttcgacg
    16021 gcgttctgtt tcgatcggct gcgttgcggt tcgctagctc tcgctcgtgc catgtgtcct
    16081 gtccctctat tgagaaaagt ctatccaagc aggtgatatc tggcaagaaa tccctgtttc
    16141 catccgtgag tccggaggcg ggaacgggcg gggcaggcgg ttagtcaacc cactcgacat
    16201 cgccgttgcc gggccgtgtc gcatttttct gatcgggagc ggcgggagga ccggctaaga
    16261 cctctcggac cagcgacaac agcgtttcgc ttgtaaaggg tttcttgaga aagggtaatc
    16321 ccgctcgcac gaggccgcga ttctgcaact cagaactcgg gctgctggac acgagcagga
    16381 cgcggatctc gcgcttgtct tgcaggagcc gctcaaccat gtcgagaccg ttcacgcggg
    16441 gataggggtt cctgtcgacg gaaagttgaa agcccggtgg aggcagaaag acatcggcga
    16501 tcacgaggtg gatcgggctg gggtgttctg catagagttt gagcgcttcg gcactgcccg
    16561 gtgcctgaag caccgtgtag ccggcatccc gcaagggttt ggtactgaca agaagggcgc
    16621 tggggtcgtc gtcgactatt aagatcgtcg agggctgttt ttgaccgggg gccatgctgg
    16681 actcctctgt tcggtgtctg tatcccgagg cctcagaatg gagggtgttg ctccttcaat
    16741 cgttcgtatt cgcgttgttc gcaaccgcca tgcttggggc gcagggattc agggacatcc
    16801 gcgaaacgga tggtgcaata gcgttgggcc tggcgtctcc ggtcgaggtc ttgttgctct
    16861 tcgagttgct gaagtcgaac ggtgaggtcg atggctggaa gatctttgcc cgtcggattg
    16921 tcctggcgtg atagaatcgg ttcatcttca ctggatctag ggtggggtcc gttcaaccgc
    16981 cagcgtgtca acacatcgtc ctgattgaac acaatgatca agccgaactg ggagtagtcc
    17041 cagatcagtt ctccctgctc cgggcgcgcc cagatggagc gaggcagacc gtatcgattc
    17101 cagatctcgt cacgcagcat gcccacccgt ggagtgagat tccggatcgc cgtgatctgc
    17161 gcctgtatcg gagaaatgtc tttctgcttc ccggggctgc tgtgtctcga gagtgattcc
    17221 agcgcggtgc gcggcacgcg cagcaatcgc cacaccgtga taagcccctg cgcgcagtca
    17281 gccggatgca ggtcttcgaa aatcatttgc gtatcgatag gaaagccgcc gacttcctga
    17341 gtacgagtga aatttttgac ctcttggacg gcggcgacgt atgcgcgttg gcgcccgtct
    17401 tctatgttag gagcgcatga agctcgccct gtgaagaaca cgtcctcccc gaaatgaaag
    17461 agcgcttgct cggtccaaaa cggacgattc tccgcagcat gtgtcgtgga ggtcaccgca
    17521 agactcaata atgtgaggag aaggtagtga gccatatgcg cggccggagc aaggacgttg
    17581 cggaacggca cccttgcagt atatcgcgtg aggcaaaaaa tacgacaaaa gagcgattat
    17641 gcacgtccag ggattgtcgg acagagattg tgagtcgggt ccgggggctg gactgtagag
    17701 ccaggcagcg gggcaatagc tagtaattca tggagggagt caaattctcc ctgcccgacg
    17761 aggcgggggc ggcgtgggcc ttcggcgggc tcatttcggt gctcatgagg ccggacccag
    17821 gcgggcaaat gctctccggc gcgacagcgc caggattgcg cagaccccag gccgttgcga
    17881 gggcatgaca ggtgaggagg ctgacccctt cctcgatatt gctgaagcgt tcggtcgatt
    17941 gagccttgcc gctccattcg atttcgccgg tatttacatc cacggccctg agggcaacgg
    18001 tggctgaata gacgcgctgg ctgtgaggaa agccgctact ccaatccagt gcttcccagg
    18061 aacccacctc ggcattgctg aagaccacca gccgagcgcc gacgagtttg gccattcgca
    18121 gcacatccgt ttccagatac tggtagccgg tgaggcttac cttctggtcg gccgccgcct
    18181 gcaacacctt cgtctggtcc acgacgagaa cgcctttttt gagcaaccat gtactcgcac
    18241 tctgaaccgt gtccggacgt ccgccccaga cgaccgccct ggtgtatggg gaggggagtt
    18301 tggagtggaa gccatccgtc gtcggggttt cgatgaacgc gtacccatgg caacccgctg
    18361 ttgccaagaa gaggagcgat aggcagatga acagattcgc cggtttcatg ggtctcttcg
    18421 gaagagcatg tgctgcgagc acgtcgggag cccaggattg taatcaagag accgttcgga
    18481 cgacggtatt atacatgaac aattctacct acgtctgctc tattcgtcga gcggactccg
    18541 ctctcgaagc agggcgaggt cctctaccct tacctactta cgcgaattgc gaaagatcgt
    18601 tcggcgcacc ggtcgagggg ctagagcggc tggaacgagt gccgaggcag gcggcaggga
    18661 tgcggaacgt gcctggaaaa gcggcggttc tgctcatggc tgacagaacc gccgcagtct
    18721 cacaggatct cgacctgcgg aaaggcgttt tgctcgcctg tcttctgggg ccggtcagcc
    18781 atgatcatga gttggatggc aatcagcggg agaagtgcat aaccgatcag gatcaacggg
    18841 aactcaatca tggtggaaac tcctttgtta tgtggagcat ccagtgcaat ctcgataccg
    18901 tgctctgctc tggccctaac acgttgattc ctatagcgct cagaagcgcc ttcctaataa
    18961 gctaagctga tgcaacgcaa gaatcgatgt agagaactgt gcaatgagaa cattttgcgc
    19021 tgagccggaa ggcctaaggg tagccgtcca aaaggtagac gccggtcggg caagcgctcc
    19081 caattcggat agaaaacaac gagcagggtt cataacgaaa tggggggcga aagcgggccc
    19141 tgccggaagt ccctgcgcgc caggcgtgag gtacaggtag gacaggaggg tgatagctcc
    19201 atgacttgga gcaatcgcga aagaatgggc gaccgcgtgg gcgagcgggt gaccgagcag
    19261 tgcaagttga agtgggatgg aatggtgccc ccgacacgaa ttgaacgtgc gacccgcggt
    19321 ttaggaaac
//
LOCUS       DAOQRM010000035        19083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2018_length_19083_cov_17.610206, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000035 DAOQRM010000000
VERSION     DAOQRM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19083
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2018_length_19083_cov_17.610206"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..806
                     /locus_tag="PKK81_04880"
     CDS             72..806
                     /locus_tag="PKK81_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV32234.1"
                     /translation="MTNKVALITGGAKGIGRAIALDLAAQQWSVAICYRTSKAAADET
                     SAAIRARGGQALAVQCDVSDPKAAGDLVSQVESRWGRIDALINGAGPYHRVNLFDETP
                     AGWREMFEGNLHPIFYLGQAVAPGMKARKQGRIINFSMANADQMVAQPEVTGHYIAKA
                     GVLILTRTLAKLLAPYGITVNAISPGFIDSGSAPPDELAGVVKRIPAGYIGSVGDTVA
                     AVRYLLSEEARYVNGTNLHLSGGWGI"
     gene            complement(816..2252)
                     /locus_tag="PKK81_04885"
     CDS             complement(816..2252)
                     /locus_tag="PKK81_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNV32235.1"
                     /translation="MSEDLHPKSPLCVDLDGTLIRTDLLWESLLALLKQSPLSVFQLP
                     FWLLKGKASFKHEIARRVTLDASTLPYDQALVEFLSNERRAGRELVLATASHESFARA
                     VAAHLGLFDERVFGSDASTNLKGARKVALLVERYGARRFAYAGNSTADLPVWAESNEA
                     IVVNASAGLVSRAQTLTPVSRVFSEPVKWLKQVAKALRVHQWAKNVLVFIPVVASHQI
                     TNRALMLQATLAFLSFSFSASAVYIVNDCLDLASDRRHPRKKYRPFASGSLSIPFGLL
                     LAAGCLVGGILLALALPSPFLLVLAGYLVLTTGYSFYLKQFVLLDVIVLAQLYTVRVY
                     GGGAATGVAPSHWLLTFSLFLFLSLALVKRFTELRLTGEAEGKELHGRGYWVTDLEHV
                     SSIGTASGLLAVLVLALYISSKEVLLLYTHADVLWLVCPVMLYWISRVWMLAYRNRMD
                     DDPVVFAVKDSKSYLMAAIIGAILFFAR"
     gene            complement(2413..2739)
                     /locus_tag="PKK81_04890"
     CDS             complement(2413..2739)
                     /locus_tag="PKK81_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNV32236.1"
                     /translation="MADPIEEEIQQEIKANKILIYGKGTKAMPMCGFTRETMHFFEKY
                     GYPYELIDVLSQPAKREALTKLTNWPTLPKVFIDGQFYGDTDILDPMEAKGEVVPLLK
                     KAFGAE"
     assembly_gap    2837..2936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2937..3269
                     /locus_tag="PKK81_04895"
     CDS             <2937..3269
                     /locus_tag="PKK81_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32237.1"
                     /translation="RLTRITLCAVLLVLGCQSTLSVRTAAAAGSGSEAIQGLGSYFLT
                     LPYGGIKMAVAVLGAVAGGMGFIFTGGDKVTAEKIWSPALGGEYVVTPAHLRGEKDLH
                     FFGSTATK"
     gene            3555..3989
                     /locus_tag="PKK81_04900"
     CDS             3555..3989
                     /locus_tag="PKK81_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV32238.1"
                     /translation="MGEFKSGFFMSESACNGRVLVVDDEPDIRKVVRMTLQKAGYEVI
                     EAENGEKAIEAINTGENRLLLDVLICDIRMPKINGVEAIAYFQQEWPRVPIIVLTGFP
                     DTDMATSFLRSGVVDYLVKPVEGEKLRTAVARAMEQRELAQL"
     gene            4121..5170
                     /locus_tag="PKK81_04905"
     CDS             4121..5170
                     /locus_tag="PKK81_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32239.1"
                     /translation="MKLCATRIAIIGAGKGGTAILDLLHQIPDVEIIGIADKDPTAPG
                     LKRARDLNVLVAERVQDLIQNHGVNLIMDVTGDPSMEPLIHTMKRPGSEVLGGAATRL
                     LWKLVQHQSKLEAELFQADKLAGLGSFAAGIAHDINNPLQLILGLAENLEEEQDLAVV
                     HEQARDITEAVKRTSAICRDLTRYARRNGSREEVMVNLNTKLDEALRIARYAVTLQDV
                     TVVKRYAEEASVMGNPDELLHVFVNLITNAVQAIKDHGTLTLQTTVGPDGLVSASVSD
                     TGCGIPKEAFSKIFEPLYTTKPPGKGTGLGLYNVMSVISKMDGHICVESDVGVGTTFR
                     IEFPQVQPTISCAPL"
     gene            5167..>6038
                     /locus_tag="PKK81_04910"
     CDS             5167..>6038
                     /locus_tag="PKK81_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV32240.1"
                     /translation="MTSTSRSFSKALGWGLKRKLIVSMLMVGVVPLLLGLSLAFLQGS
                     KEIQVVSGESFQALATEAARKLDLLVAEEVARTSRIAVHPDIVRALEHRRDALHASDQ
                     NATLPTLVQQRARWAARDPAAIKAITGNSTALLLQGFYAGSQSEPDQLLPQVVRAATK
                     KLFITDVQGNLFAALTTKPGFSHADSPWWKGSFNRGVGQLYIEDLHFDEQANAYVFSI
                     SLPVMDSLRYEVVGVLHRVIDAKEFFSPSTHPIRFGKTGHVMLIDSRGMVLSCPILPT
                     GVQLSGASLIPQVTV"
     assembly_gap    6039..6138
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6139..7479
                     /locus_tag="PKK81_04915"
     CDS             <6139..7479
                     /locus_tag="PKK81_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32241.1"
                     /translation="VRRLQEAARLIGRGELQEPIQIHTGDELEELAVEFNRMNTQLEA
                     AFAGLSTQVEEKTQEVQYLQKSTDQVLDAVPTPIIMIDQQGFVQYMNRASRDAIQPQE
                     QSWSGRLLFDLLPLDDDHLQALRRDLRLVEENKPTAPPRPDQEPALKEARDPLNQTLS
                     QASPSARRELRIGAHLYHYEWFPLETRSGAGKRFGLVLRDTTDESRLQDQLIQAEKSG
                     SLDVLTAGIGHELNNPLFGILGLGEAIQEEADLARAKGYAQDIVGHGRKMAAIIRDFT
                     GVAARESKSQRVPVDVTAQLEQALAIVQTSHDCLGLNVQKHYVAVSPVTALPDQLRLA
                     FINVLTNAIQAMSGRGDLWLTTEEQDDLITITIRDNGPGIPKHHLGKVFDPFYTTKGQ
                     GEGSGLGLTVAQRLIKKFGGEIRLESPEGQGTTCVITLPADKAGVRKEEPCVSP"
     gene            7464..>7985
                     /locus_tag="PKK81_04920"
     CDS             7464..>7985
                     /locus_tag="PKK81_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV32242.1"
                     /translation="MRLTLTVIRARPLWLALALACASSYSLQAKDIAPPPGIPPEKVA
                     DFVHAVLDADRTIYTNQIVNRMQAKGIVSAAEHWEHENALPLPAQFLQHSGKLVAESG
                     RGIRYRLISLWPIYQRNAPATDLERRALESFSQTPDRPFTGIVTSGRKQYFQAIYSDR
                     AISAACVKCHNSHP"
     assembly_gap    7986..8085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8086..8903)
                     /locus_tag="PKK81_04925"
     CDS             complement(<8086..8903)
                     /locus_tag="PKK81_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNV32243.1"
                     /translation="MNRQQIFALCFFGVLLALLYQMGLMFRPFVFPVLWAVILAHLCF
                     PLHIRLTAWLGGRESHSAVLLTLAALALVVVPLVVLSVMLVKEAGSADRAVREWIASG
                     GVQQLPDRLSGLPGGPVVREWLERFTGRESDLEQFVLSSAKTFSRFIVGELSDLVRDV
                     FMLVADFLVMMFTLFFFFKDGRHWLASLYALIPLEASHKDKILARLDQTIRAVVKGIV
                     LTAIAQGLLAGAAYAVMGVPFPMVLMALTILLAPLPFGGTALIWGPVALYLLWAG"
     gene            complement(9078..9518)
                     /locus_tag="PKK81_04930"
     CDS             complement(9078..9518)
                     /locus_tag="PKK81_04930"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV32244.1"
                     /translation="MMKKSGFVENDNITLLAKGVLLRGEIRVEGTVRIDGRLEGDLHT
                     TGTVVIGEDGIVQGTITAGIIISSGKIKATVRATERLQLLKTGLLVGEVHAPAFSMED
                     GAKFQGTSDMGVASWGDEAQKLPGNVRDISSQRPKAVAMLGENA"
     gene            complement(9611..>11650)
                     /locus_tag="PKK81_04935"
     CDS             complement(9611..>11650)
                     /locus_tag="PKK81_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNV32245.1"
                     /translation="PGEYRVTPAEIDIYLHEFPDFHLRPVPARLALDQGRVTKVLSIP
                     GGDELFPAYLEPQLISGLRGASRQVREWVSYDDLSARFLDVLLAIEDRRFFSHPGIDP
                     IAVGRAVWTNVTRGTVIQGGSTITQQLAKNLFYSPQRTFGRKLKESMAALVLEAKYRK
                     QAILESYANEIYLGQVGSVSIYGVGEAAHRYFGKRVDALSLEETALLVGMIKGPNTYS
                     PLRNPALAKQRRDVVLGRLHEQGLVSDDAWKQAVMTPVRVMPPQDTLADAPFFVDHLL
                     RQVEETTGAPLPDGVRAYTTLDPVLQRLATQTLESELGKLEAAYPALTGHSSPVQAAL
                     VALDPATGGILAMVGSRDYRTSQFNRAVQSKRQPGSLFKPVVYLAALEARRELSGGQP
                     ITAATSIVDEPVTFESGTGVWAPQNYDHRFHGTVSVRTAIEQSLNIPAVKIAQAVGTK
                     RILQMAEKLGIRSPLADNLSIALGTSGVSLLEITSAYGALAQGGLYVAPSALQAVMTP
                     EGDPAWHRTPVRHQAVSPQAAYVMTSLLRGVVERGTAAKAKAMGLRGTVAGKTGTTDG
                     YRDAWFVGYTPDVVVGVWVGFDDEEALHLTGAQAALPMWVEFVRRISPAATREFALPP
                     AVVTRDIDPQSAQLATSKCPQRSAELFIEGTEPSIYCEVHGSGFWERVKRSFGFS"
     assembly_gap    11651..11750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12198..13523
                     /locus_tag="PKK81_04940"
     CDS             12198..13523
                     /locus_tag="PKK81_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNV32246.1"
                     /translation="MTAFLPPAARAGSFRIYDHSASATGQASAFTAQADDASAGYYNP
                     AGMTQLRGVQFSAGTTLIAGGFSFQNGAGTQANGDLRGSVAVPPPSNVYMTANLKDLG
                     IGSSDSTTIGLAVFSPFGTLTRWPDSSPFSTATTKAAFELIDIRPSIAFRPLPDLAIG
                     LGADIYTFSGAFGEGQVERQFRWPGGLGIPAGTGMELNGRDTAAGFNASLLYTPLRNG
                     DGRPLVNIGLIYRSQATLHLDGQLLAGGTRVADARTTFVVPQVLTGGIALWPVRDKER
                     EWKLELDVDYTGWKSVRNLDTTLSNGLTIATPANWTSGYTVMVGTEYKWLNPAPLPDW
                     DVALRAGYWHSQKAIPDQTFNPAIPDGDNHAVSTGFGFMCREHGKFLGVIPCGGSDKA
                     LSPKGLGIDLAYQAILYEGRTVAGNVNPTVNGTYRTTLHVGSINLRVNF"
     assembly_gap    13796..13895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13896..15710
                     /locus_tag="PKK81_04945"
     CDS             <13896..15710
                     /locus_tag="PKK81_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV32247.1"
                     /translation="AWNFNRVLLSSMRLQLEKVALARSLTTRTEAVERLNQDITKEIH
                     DRRVIEEQLRTAQTDLEQRITERTADLAQANARLQNEVREHRRTEGARLSSEKRFNYL
                     TDNLNQGVWFAQANPPQVLYVNPAFERIWGLPAARFYENPKLWRDFIHPDHQQVVSEV
                     YDAEIANPTGRDLHLLYRIVRPDGQVRWIHDRMVIHHTATGEVDSLSGITEDITEARE
                     LEDQLRQAQKMEAVGRLAGGVAHDFNNVMTVILGYSAVLLQELSQNSSARYFVQEIQR
                     AGERCAALTGQLLAFSRKQMLHPVSLDLHRVIRDLMALLKSLIGEHISIVLQLDPLPL
                     WVKADAVQLEQVLLNLAVNARDAMPHGGTLTIDTRQVSREAVWGSDHDTRTARTYVRL
                     RVHDTGTGIDPATKAKIFEPFFTTKPPGRGTGLGLSTVYGIVHQSGGTITVESAVGQG
                     TTMTVFLPEVVPPLTALPPVQSEPAHTTGTEIILLVEDEPAVRLLTQHILRTHGYTVH
                     EAEDGFQALDLIRRNALHIDLLLTDLVMPGMNGKELAMRLRSHFAELKVLYMSGYSDN
                     PPVTGDEAQGHTTFLQKPFSPEDLIRMVREILHSVSPA"
     gene            complement(15897..17360)
                     /locus_tag="PKK81_04950"
     CDS             complement(15897..17360)
                     /locus_tag="PKK81_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNV32248.1"
                     /translation="MLLSFVILYLACSVGIGLYAATRVHTSKDFAVAGRCLPLPVVTA
                     TVFATWFGAETVLGISATFVKDGLRAVVADPFGSSLCLILAGLLFARRLYRLNLLTIG
                     DFYRLRYNRTVEVLCTLCIVASYLGWVSAQIKALGLVFNVVTDGAMSQSVGMVLGAII
                     VLTYTTFGGMFSVAILDFVQITIIMGGMLYIGSVISGLAGGVERVVTHAAMAGKLDFF
                     PPARMEAWIPFLGAWVTMMFGSIPQQDVFQRITSARDERTAVRGSVLGGTLYFFFAFV
                     PMFLAYSATLVDPAQVAQLLERDSQLILPNLIVQHTPIVAQIIFFGALLSAIMSCSSA
                     TLLAPSVAFSENIVKGWVPTISDQGLLRVMRIVLVGFAATVLLFALNSEASIFKMVES
                     AYKVTLVAAFVPLLAGLYWARATTQGAVLAITVGLGSWCGSELTVTLDQIWPPQLVGL
                     LAAAVGMVAGSLLPQWIEHPAGLSASGNRPSLSSPVE"
     assembly_gap    17513..17612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17877..18107
                     /locus_tag="PKK81_04955"
     CDS             17877..18107
                     /locus_tag="PKK81_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32249.1"
                     /translation="MRYSFYFAWVVAVLSICVLTTLASFPLLVNGERPPEPRITAGAE
                     KLRTIKIHRLRCNPFTPSCQLSKHKPSHKVPA"
     gene            complement(18129..>19083)
                     /locus_tag="PKK81_04960"
     CDS             complement(18129..>19083)
                     /locus_tag="PKK81_04960"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HNV32250.1"
                     /translation="EMGQKQVIAVVGATGMQGGGLVRAILADPSSGMTVRALTRDVNS
                     DKARELARLGAEVVAADVHDGESLKRAFAGAAGVFCVTFFWAHFSPEKEFAEAEAMAK
                     AAKSAGVPHVIWSTLEDTRRWVPLSDNRMPTLMGKYKVPHFDAKGEADQVFRQLGVPT
                     TFLLTSFYWDNLIHFGMGPKKGPDGTLAFTLPMGEARLPGIAAEDIGKCALGLLKKRD
                     AYLGKTVGIAGEHLTGSQMAVALTKALGQGVRYNAVPPEVYRTFGFPGADDLGNMFQF
                     KRDFNALFCGAREPAIARALNPGLQTFAQWLEANKGRIPLS"
BASE COUNT         4005 a         6002 c         5224 g         3252 t
ORIGIN      
        1 tacggtgagc ctcggcgcga tgcgagaacg ccgctggcgg actttctcaa cagcctgctc
       61 acggagtccc tatgacgaat aaagttgccc tcatcaccgg cggcgccaag ggaatcggcc
      121 gcgccatcgc gctcgatctc gccgcgcaac aatggtccgt ggcgatctgc taccgcacca
      181 gcaaagcagc cgcggatgaa accagcgcgg ccatcagggc acgcggcggg caagccctgg
      241 ccgtgcagtg cgacgtctca gacccgaagg ccgccggcga cttggtctca caggtcgaaa
      301 gccggtgggg aagaatcgac gcactgatca acggcgccgg cccctatcac cgcgtcaatc
      361 tcttcgacga gacgccggcg ggctggcgtg aaatgttcga gggaaactta cacccgattt
      421 tttatcttgg gcaggcggtc gcacccggga tgaaggcacg gaagcaggga cgcatcatca
      481 acttcagcat ggccaacgcg gaccagatgg tagcccagcc ggaggtgacg gggcactata
      541 tcgccaaagc cggcgtgtta attctcacca ggacactggc aaaactgctc gccccctacg
      601 gcattaccgt caacgccatc tcccctggat tcatcgattc gggcagcgcg ccgcccgatg
      661 aactggccgg tgtggtcaaa cgaattcccg ccggatacat cgggagcgtg ggggatacag
      721 tcgcggccgt gcgatacctg ctcagcgagg aggcgcgcta cgttaacggc accaacctgc
      781 acctcagcgg cggatggggc atctgactcg acggtttacc tggcaaagaa gagaatggct
      841 ccgatgatcg ccgccatgag ataacttttt gaatccttga ccgcgaacac caccggatcg
      901 tcgtccattc gattccggta ggccagcatc cagacccgac tgatccagta gagcatcacc
      961 ggacagacca gccataatac gtccgcatgc gtgtacagca gcagcacttc tttactactg
     1021 atgtagagtg ccaacaccaa caccgccagc aatccgctcg ccgtgccgat actcgacaca
     1081 tgttccagat ccgtcaccca ataccctcgc ccgtgcagtt ccttgccttc ggcctcaccg
     1141 gtaagccgaa gttcggtaaa ccgcttcacc aaagcaaggc tcaggaagag aaacaacgag
     1201 aacgtcagca gccaatggga gggagccacc cccgtcgcag ccccgcctcc gtatacccgc
     1261 acggtgtaga gttgggccaa cacaatcaca tccagcagca cgaactgttt cagatagaac
     1321 gaataccccg tcgtcagaac caggtagccg gccagcacga gcagaaatgg cgagggcagt
     1381 gccagcgcca gcaaaatacc ccccacaaga caccctgcag ccaagaggag cccgaacgga
     1441 atcgagaggc tgccggaagc aaagggacgg tattttttcc ggggatgccg gcggtcggac
     1501 gcgaggtcga gacagtcatt gacgatatag acggcagacg ctgagaaact gaatgacagg
     1561 aatgccaggg tggcctgcag catcagggct cggttggtga tttgatgcga ggcgaccacc
     1621 ggtataaaca ccagcacatt cttcgcccac tgatgtacgc gcaaggcctt agccacctgc
     1681 ttcagccatt tcaccggctc gctgaacaca cgactcaccg gggtcagcgt ttgcgcgcgg
     1741 ctcaccaatc cggcagaggc attcacgacg atcgcttcgt tagactcggc ccacacgggc
     1801 aaatcggcgg tcgaatttcc cgcataggca aagcggcgag ccccgtatcg ctcaaccagg
     1861 agggcaacct tgcgtgcgcc tttgagattg gtcgaagcat cgctcccgaa cacccgctca
     1921 tcgaacaacc cgagatgagc cgctacggca cgggcaaaac tctcatgact ggctgtggcc
     1981 agcaccagct cacgcccagc ccggcgttcg ttggagagaa actcgaccag agcctggtcg
     2041 taggggagcg tgctagcatc gagcgtcaca cggcgggcaa tttcatgctt gaaagaggct
     2101 ttgcctttca gcaaccagaa gggaagctga aagaccgaga gcggactctg cttcagcagc
     2161 gccagcagcg attcccacag cagatcggtc ctgatgagcg tgccatcaag atcgacacac
     2221 aagggacttt tggggtggag atcctcactc atgggacgca ggagggattc tatcggcaag
     2281 ggggaatctg ggcaagcaca tacgacgaac ttcgagggag tgaagatctg gtatgccgga
     2341 cggggatcga accgactgcc cctgaaagcc ggctacggat tggagagagg agtggacgac
     2401 ggctcagcgc gcttattcag ctccgaacgc cttcttcagc aacggcacca cttcgccctt
     2461 ggcttccatg gggtccagaa tatccgtgtc gccgtaaaac tgtccgtcga tgaacacctt
     2521 cggcaaagtc ggccagttcg tcagcttcgt caacgcctcg cgtttggcgg gctgggacaa
     2581 cacgtcgata agctcgtagg gatacccgta tttttcgaaa aagtgcatgg tctccctcgt
     2641 aaacccgcac atcggcatgg ctttggtgcc cttgccgtaa atcaaaatct tgttcgcctt
     2701 gatttcttgc tgaatctctt cttcgatcgg gtcagccatc tgtccctcct ctactattac
     2761 tatgtgttca cttcttccgg tgtcttggcg gtaatctcca aggcatggat acgtccgtct
     2821 ttcatcggag cggccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcc
     2941 tgacacggat cactctgtgc gccgtactgc ttgtcctcgg atgccagtcg accctgtccg
     3001 tgcgaacagc cgcagccgca ggctccggat cggaagccat tcaaggattg ggaagttact
     3061 tcctgacgct gccgtatggc ggcatcaaaa tggccgtcgc cgtgctcggc gcggtcgccg
     3121 gaggaatggg gtttatcttc accggcgggg acaaagtgac cgctgaaaaa atctggagcc
     3181 cggcccttgg cggagaatat gtcgtcaccc ctgcacatct ccggggcgag aaggacttac
     3241 atttcttcgg aagtaccgcg accaagtaac gcctcttttt tcagccggac cggatatcga
     3301 ccggtccact catcaacacc caatggcctt tcgaccgttc cggtttcccg atctgatcgg
     3361 cgagccggtg gtgcatttga cccggcctgg tggttatcaa ccaccaggcc ggaccccctc
     3421 ctcacttact tttcctaaca tactgaaatc acgatcttct tcaggcttcc ttcattggca
     3481 tgtgtgttgc tgttactcag cagcaagtac ggcagatgca aacaacaaac gacacaacca
     3541 tcaaggaggg gatcatggga gaatttaaat cgggtttctt catgagtgag tccgcctgca
     3601 acggtcgagt gctcgtcgtc gatgatgaac cggacattcg caaagtcgtc cgaatgacct
     3661 tgcagaaggc cgggtatgag gtcatcgagg cggagaacgg ggaaaaagcc atcgaggcca
     3721 tcaacaccgg agaaaaccgg ctgctgctgg acgtcctcat ctgcgacatc cgcatgccga
     3781 agatcaacgg ggttgaggcc attgcctact tccagcagga atggccgcgt gtgccgatca
     3841 ttgtcctgac cggcttcccc gacaccgata tggcaacctc gttcttacga agcggcgtcg
     3901 tcgactacct cgtgaaaccg gtcgaaggag aaaagctacg cacggccgtg gccagagcca
     3961 tggaacagcg ggagcttgcc cagctctagt gacgcccatg ccgacagacg gcggcacacc
     4021 ctccacgtcg gggaggcata ccaggcctcc ccgacggcaa ggtgcgagtg ggtgaggctc
     4081 gcctcggacc ggacctcacg acacatcatg ggaagcgact atgaaacttt gtgcgacgag
     4141 aattgccatc atcggcgccg gaaaaggcgg aaccgcgatc ctggatctcc ttcatcaaat
     4201 cccggacgtg gagatcatcg gcattgcgga caaggatccg acggctcccg gactcaagcg
     4261 ggcgcgagat ctgaatgtgc tggtcgcgga acgggtgcag gatctcatcc agaatcatgg
     4321 cgtcaatctg atcatggatg tgaccggcga cccatccatg gaaccgctca tccacaccat
     4381 gaagcgtccc ggctccgagg tcctcggcgg agccgccacc agactgctct ggaaactcgt
     4441 tcaacaccag tcgaaattgg aagctgaatt gttccaggcc gacaaactgg ccggactggg
     4501 ctctttcgcg gccggtatcg cgcacgacat caacaatccg ctgcagttga ttctcggact
     4561 ggccgaaaat ctggaagagg agcaggacct cgcggtcgtc catgaacagg cccgcgacat
     4621 caccgaggcc gtcaaacgca ccagcgccat ctgtcgcgac ctgacccgat acgcaagacg
     4681 caacggctcc agggaagagg tcatggtcaa cctcaacacc aagctggacg aagcgctgag
     4741 aatcgcccgt tatgcggtga cgctccagga cgtgaccgtc gtcaaacggt atgcggaaga
     4801 agcgagcgtg atgggcaatc ccgacgaact ccttcatgtc ttcgtcaatt tgatcacgaa
     4861 cgcggtccaa gccatcaagg atcacggcac attgacgctc caaaccacgg tcgggccgga
     4921 cggattggtc agtgcctcgg tcagcgatac ggggtgcggc atccccaagg aagccttctc
     4981 gaaaatcttt gaaccactct acaccaccaa acctcccggg aaaggcacgg ggttggggtt
     5041 gtacaatgtg atgtcggtga tctcgaaaat ggacggtcac atttgcgtcg aaagcgacgt
     5101 gggcgtgggc acgaccttcc gcatcgaatt tccacaggtg cagccgacca tatcatgcgc
     5161 gcctctatga caagtacatc gcgctccttc tcgaaggccc tcgggtgggg cctgaaacgt
     5221 aagctcatcg tctccatgct gatggtcggc gtggttcccc tcctgctcgg cctcagcctg
     5281 gccttcctcc aaggatcgaa agaaatccag gtcgtcagcg gggaaagctt tcaggcgctg
     5341 gccacggagg cggcccgcaa attagacctc ctcgtggcgg aggaggtggc caggacctcc
     5401 cgcatcgcgg tccacccgga tatcgtgcgt gcgctggaac accgtcgcga cgcgctccac
     5461 gcctccgatc agaacgctac gttgccgact ctcgtgcaac aacgggcccg ctgggcggca
     5521 agagacccgg ctgcgatcaa agcgatcacg ggaaattcca ccgcccttct cttgcaaggt
     5581 ttttacgccg gctcgcagag cgaaccggac caactcctgc cgcaggtcgt gcgcgccgcc
     5641 accaagaagc tgttcatcac ggatgtgcag ggaaacctct ttgccgccct gacgaccaaa
     5701 cccggcttct cccatgccga cagtccctgg tggaaaggct cgttcaacag aggagtcggc
     5761 cagctctaca tcgaagatct tcacttcgac gaacaggcca atgcctatgt cttcagcatt
     5821 tccctgccgg tcatggacag ccttcggtat gaagtggtgg gcgtgctcca ccgggtcatc
     5881 gatgccaagg agttcttctc tccctcgacc caccccatcc ggttcggcaa gaccggccat
     5941 gtgatgctca tcgatagccg aggcatggtc ctcagctgcc cgatcctgcc gaccggcgtt
     6001 cagctctcag gcgcatcgct gattccccag gtgacggtnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnngt cagacgactg caggaggccg cgcgcctcat cgggcgagga
     6181 gaattgcagg agccgatcca gattcacacg ggagatgaac tggaagagct ggcggtcgaa
     6241 ttcaaccgca tgaacacgca actcgaagcc gcctttgccg gattgagcac ccaggtggaa
     6301 gaaaaaacac aggaggtgca gtacctccaa aaatcgacgg atcaggtgct cgacgccgtc
     6361 ccgaccccga tcatcatgat cgaccagcaa ggcttcgtcc aatacatgaa ccgcgcgtcc
     6421 cgggatgcca ttcagccaca ggaacaaagc tggtcgggcc gtctgctctt tgatcttctc
     6481 cctctggacg acgatcacct tcaggccttg cggcgagacc tacgactcgt tgaagaaaac
     6541 aaaccgaccg cgcccccccg cccggaccag gaacccgccc tgaaagaggc gcgcgaccca
     6601 ctcaatcaaa ccctcagcca ggcgtccccc tcagccagac gggaattacg gatcggcgcg
     6661 cacctgtacc actatgaatg gtttccgctg gaaactcgta gcggggcagg caagcgcttc
     6721 ggcctcgtgc tgcgcgatac caccgacgaa agccggctgc aggaccagct gatccaagct
     6781 gaaaaatcag gcagcctcga tgtgctgacc gccggcatcg gccacgaact caacaacccg
     6841 ctgtttggaa ttctgggcct cggggaggcg atccaggagg aagcggacct ggctcgcgcc
     6901 aagggttacg cgcaagacat cgtcggacac ggccgaaaga tggcggccat cattcgggac
     6961 ttcaccggag tggccgcccg tgaatcgaaa agccaacgtg tgccggtgga tgtgaccgcc
     7021 cagttagaac aggccctggc cattgtacag acctcgcacg actgcctcgg cctgaacgtc
     7081 cagaaacact atgtggcggt gtcgccggtg acggcgctgc ccgaccagct tcgcctggca
     7141 tttatcaacg tcctcaccaa tgccattcag gccatgagcg ggcggggcga cctgtggctg
     7201 accaccgaag aacaggacga cctgattacg atcacgatac gggacaacgg ccccggcatt
     7261 cccaaacacc atctcggcaa agtcttcgat cccttctaca ccaccaaggg acagggagaa
     7321 ggctcgggac tcggcctcac cgtcgcgcaa cggctgatca agaaattcgg cggcgagatt
     7381 cggctggaga gtccggaagg ccagggcacg acctgcgtca tcaccctgcc ggcggacaag
     7441 gccggggttc gaaaggagga gccatgcgtc tcaccctgac agtcattcgg gcacggccgc
     7501 tgtggctcgc actggcgctg gcctgcgcct cgagctattc gttgcaggcc aaagatatcg
     7561 cgccgccgcc gggcattcct ccggagaagg tcgcggactt cgtgcacgcg gttctggatg
     7621 ccgaccgaac catctacacg aaccagatcg tcaaccggat gcaggccaaa ggcatcgtgt
     7681 ctgccgcgga acattgggaa cacgaaaatg ccctgccgct gcctgcccag ttccttcaac
     7741 attcgggcaa actcgtggcc gagtccggac gaggcattcg ctatcgtctc atcagcctct
     7801 ggcccatcta ccagcggaat gcccccgcca ccgacttgga acgccgggcg ctggagagtt
     7861 tttcgcagac ccccgaccgg ccctttacgg gcatcgtgac cagcggccgc aaacaatact
     7921 ttcaggccat ctattccgat cgggccatct cagccgcctg cgtgaagtgt cacaacagcc
     7981 acccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccggc ccagaggaga
     8101 tacaaggcca caggccccca gatgagcgcc gtcccgccga acggaagcgg agccaggaga
     8161 atcgtcaggg ccatcaacac catgggaaat ggcacaccca tcaccgcata ggccgctccg
     8221 gcgagtagcc cctgcgcaat ggccgtcagc acgatccctt tcaccacggc gcgaatggtc
     8281 tgatccaggc gagccaggat cttgtccttg tgcgaggcct ccagcgggat caacgcatac
     8341 agcgacgcca gccagtggcg gccatctttg aagaagaaaa acagggtaaa catcatgacg
     8401 agaaagtcgg cgaccaacat aaagacgtcc cgcacaaggt cgctcaactc gcccacgatg
     8461 aatctactga aggtcttcgc gcttgagagc acgaattgtt cgagatcact ttccctgccg
     8521 gtgaaccgct ccagccactc cctcaccaca gggcctcccg gtagcccgga caaccgatcg
     8581 ggcaactgct gaacgcctcc tgaggcaatc cattcccgca ccgcgcggtc agcggatccg
     8641 gcctccttca ccagcattac actcagcacc accagcggca ccacgaccag ggccagcgca
     8701 gccaatgtca ggagcacagc cgaatgcgac tcccgtcctc ccaaccaggc ggtgagccgg
     8761 atgtgcaggg ggaaacacag atgcgcaaga atcactgccc agaggaccgg aaacacgaag
     8821 gggcgaaaca tcagccccat ctgatacagc agcgccagca gcacgccgaa aaagcagagc
     8881 gcaaaaatct gttgtcgatt catagtcgtg aagagtacct gcgattgcag ccgttgatag
     8941 cagaacctgc gcaggatgtc acgcgtcggt ctgacgaccg ctgtgcctga acagaggact
     9001 gccgccaggc tgggaggaga ccctacgatg ccatagggcg cacggggtgc gcagcgtgag
     9061 ggcgggaaag cgagccgtca ggcgttttca ccgagcatgg cgacggcctt gggacgctgc
     9121 gaggaaatat cccgaacatt gccggggagt ttctgggctt cgtcacccca ggaagcgacg
     9181 cccatatccg acgtcccttg aaacttcgcc ccgtcttcca tagagaaggc gggcgcgtgg
     9241 acttcgccga cgagcagacc ggtcttgagc aattggagcc gttccgtcgc ccgcaccgtc
     9301 gccttgatct ttccactgct gatgatgatc ccggctgtga tggttccctg cacgatgccg
     9361 tcttcgccga tcacgacggt ccccgtggtg tgaagatcgc cttccaaccg cccgtcgata
     9421 cggaccgtgc cttcaacgcg aatctcgcct ctgagcaaga cccctttggc caacagggtg
     9481 atattgtcgt tttccacaaa gccggacttc ttcatcatca cgcgctcctt ctcccaggca
     9541 atgtccgatg aacttgaact ggaggaccat cccaacatga gccgagccta caccagcttg
     9601 tgcatcgtac ctaggaaaaa ccaaaactgc gcttgacccg ttcccagaag ccgctgccgt
     9661 gtacttcgca gtagatcgaa ggctccgtac cttcgataaa caactccgcc gaccgctgcg
     9721 gacacttcga ggtcgccaac tgggccgact ggggatcgat gtcgcgcgtg accacggcag
     9781 gcggcaacgc aaactcgcgc gtcgcagccg gcgagatccg gcgcacgaac tccacccaca
     9841 tcgggagcgc cgcctgcgct ccggtcagat gcagcgcctc ctcgtcgtcg aatcccaccc
     9901 acacaccgac caccacatcc ggcgtgtacc cgacgaacca ggcgtcgcga tacccatccg
     9961 tcgtcccggt ttttcccgcc acagtcccgc gcaggcccat cgccttcgcc ttggccgccg
    10021 tgccgcgctc gacgacaccc ctgagcaacg aggtcatgac ataggccgct tgcggggaca
    10081 ccgcttgatg ccgcactggc gtacgatgcc aggcgggatc cccttccgga gtcatcacgg
    10141 cctgcaacgc cgagggagcc acatagagcc ccccttgtgc cagggctccg taggccgagg
    10201 tgatctccaa cagcgagacg ccggaagtcc ccaaggcaat ggacagatta tcggccagcg
    10261 ggctgcgaat ccccagcttc tctgccatct ggagaattcg cttcgtcccc acggcctgtg
    10321 cgatcttcac ggcgggaata ttcagcgatt gttcgatcgc cgtccgcacg gacaccgtgc
    10381 cgtgaaaccg atggtcgtaa ttctgcggag cccacacccc cgtacccgac tcaaacgtca
    10441 ccggttcatc gacgatcgag gtggccgcag taatcggttg cccaccgctt aattcgcggc
    10501 gagcctccaa cgccgcgaga tacaccaccg gcttaaacaa cgagccgggc tggcgtttcg
    10561 actgcacggc ccggttgaac tgactggttc gataatcacg actgcccacc atcgcaagaa
    10621 tgccgcccgt tgcgggatcg agcgccacca gcgcggcttg caccggactg ctgtggccgg
    10681 tcagcgccgg ataggccgct tccagtttgc ccagttcact ctccagcgtc tgagtggcca
    10741 gccgttgcaa cacggggtcg agcgtcgtat aggccctgac gccgtccggc aagggcgcac
    10801 cggtcgtctc ttccacctgt ctcaacagat ggtcgacaaa aaacggcgca tcggccaatg
    10861 tgtcctgcgg cggcatcacc ctgaccggcg tcatcaccgc ctgcttccac gcgtcgtcgc
    10921 tcacgagccc ctgttcgtgc aaccggccca gcaccacatc gcgacgctgc ttggccagcg
    10981 ccggattcct caggggtgaa taggtattcg gccccttgat catcccgacc agcagcgcgg
    11041 tctcctccaa gctcaatgca tccacgcgtt tgccgaaata ccggtgcgcg gcctccccga
    11101 ctccataaat cgacacagac ccaacctgcc cgagataaat ctcattcgca taactttcga
    11161 ggatcgcctg tttgcggtat ttcgcctcca ggaccaatgc cgccatcgat tccttgagct
    11221 tccgcccaaa cgtgcgttgc ggagaataaa acaggttctt cgccaattgc tgggtgatcg
    11281 tactgccgcc ctggatgacc gtgccgcggg tcacattcgt ccagaccgcg cggcccacgg
    11341 caatgggatc gatgcctgga tggctgaaga atcgccggtc ttcgatcgcc aacaacacgt
    11401 caagaaatcg cgccgacaga tcgtcatacg acacccattc ccgcacctgc cgcgaggcgc
    11461 cgcgcaaccc gctgatcaat tgcggttcca gataggcggg gaataattcg tcgccccccg
    11521 gaatcgacag gaccttcgtc actcgcccct ggtcgagcgc caaacgggcc ggaaccggtc
    11581 gcaggtgaaa atcgggaaac tcgtgcaagt aaatatcgat ctctgccgga gtgacacgat
    11641 actcgccggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcaccaccac
    11761 gtccccatct gccgagcata cgcgatccag cgaacgaaat cacatgcgtg cacaccgctt
    11821 caggaagatc ctccggggca cccaagcttg acaaagcacc gtgccttctc ttagatgagc
    11881 gccacattgc cgaatttcta cgcccttgca cccatcggga cgtccgaaat acgcgtcggc
    11941 tctgcgcgtc acacccgtac aacgttactc gcacacgcca cgcgcccgga caggctgatt
    12001 gcgttactga cagactcatc cacacgagga atatgaccgt gagtactcta cgccttcttg
    12061 atgccggtga ttcacaggaa ccctgcgtgc ccgcgacacg gccgtcgcac cacgagagaa
    12121 cccgacggca gcgccggtgg aagcgacacg atgacgcctt cccccggctg ctcatcgcct
    12181 gttgcgcgct gattgccatg accgcgttcc tcccaccggc agcccgggcg ggaagttttc
    12241 gcatctacga tcacagtgcc tcggcaaccg gccaggcgtc ggcattcacg gcgcaagccg
    12301 acgatgcttc cgccgggtac tataatccgg ccggcatgac acaactccgc ggggttcagt
    12361 tctcagccgg caccactctt atcgccggcg gcttctcgtt tcagaatggc gcgggcaccc
    12421 aggccaacgg cgatcttcgc gggagtgtgg cggttccgcc tccgtcgaac gtctacatga
    12481 cggccaatct caaggacctc ggcatcggct cctccgacag caccactatc ggcttggccg
    12541 tcttcagccc gttcggcacc ttgacgcgct ggccggacag cagtcccttt tccacggcga
    12601 ccacgaaagc cgcgttcgaa ttgatcgaca ttcgcccctc catcgccttc cggccgctgc
    12661 cggatctggc aatcgggttg ggagcggata tctacacctt ttccggagcc ttcggagaag
    12721 ggcaggttga gcgacaattt cgctggcccg gcgggctcgg catcccggcc ggcaccggca
    12781 tggaattaaa tgggcgcgac acagccgcgg gcttcaatgc cagcctgctc tataccccgc
    12841 tgcgtaatgg ggacgggcgt ccgctcgtca acatcggcct catctaccgc agtcaggcga
    12901 cgctgcatct cgacgggcaa ttgctcgccg gcggcacgcg tgtggcagac gcccgcacga
    12961 cctttgtggt tccgcaggtt ctcaccggcg gcatcgcgct ctggcccgtc cgggataaag
    13021 agcgggaatg gaagctggaa ttggacgtgg attacaccgg ctggaaatcg gtccgcaatc
    13081 tggatacgac cctgtcgaac ggtctcacca tcgccacccc cgccaactgg accagcggct
    13141 acaccgtcat ggtcggcacg gaatacaagt ggctgaaccc cgcaccgctg cccgattggg
    13201 acgtcgccct gcgcgcaggc tactggcatt cccagaaggc gattcccgat cagaccttca
    13261 accctgccat tcccgacggc gacaaccatg ctgtctccac cggcttcggg ttcatgtgcc
    13321 gggaacacgg caagttcctc ggggtcatcc cctgcggagg atccgacaaa gccctcagcc
    13381 ccaaaggcct cggaatcgat ctggcctacc aggccattct gtacgaaggt agaaccgtcg
    13441 ccgggaacgt gaatcccacc gtcaacggaa cgtatcgcac gaccttgcat gtgggctcca
    13501 tcaatctccg tgtgaatttt tgatgcccgg ccttccaacg atcatggtag agtaggctta
    13561 cttacgagac acgcgcacat cgtgatgctg ccatcggtgc tctgtgcgcg gtgaacctgc
    13621 ttcagagcga ggccttcgta tggatcgccc atcccacacc gatccggccc cggatctgca
    13681 caccctacaa gttcaggtct tgtaccgcaa tatgcctacc ggcgtgttgg cgagcgccat
    13741 caatgccgcc gttctcgcgg cggtggaatg gtccgtcgtt ccgcatacgc ccctgnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcctg gaacttcaac cgcgtgctgc
    13921 tctcctcgat gcgcctgcaa ctggagaagg tggccctggc acggtcgctg acgacccgca
    13981 ccgaagcggt ggaacggctg aatcaggaca tcaccaaaga gatccacgac cggcgcgtca
    14041 tcgaagagca actacgcacc gcgcagaccg atctggaaca gcgcatcacc gaacgcacgg
    14101 ccgacctggc ccaagccaac gcgcggctgc agaatgaagt ccgggaacac cggcggacgg
    14161 aaggcgcgcg actttcgagc gaaaagcgat tcaactatct caccgacaat ctcaaccagg
    14221 gcgtgtggtt tgcgcaggcg aatccgccgc aggtcctgta cgtcaatccg gccttcgagc
    14281 ggatctgggg attgcctgcc gcgcgattct atgaaaatcc gaagctctgg cgggacttta
    14341 tccacccgga ccaccaacag gtcgtcagcg aggtctacga tgcggagatt gcgaatccca
    14401 ccggacgcga cctgcacctg ctgtatcgga tcgtacgtcc ggacgggcag gtccgatgga
    14461 ttcacgaccg gatggtcatc caccataccg cgacggggga agtcgatagt ctgagcggga
    14521 tcactgaaga cattacggaa gcccgcgaac tggaagacca gttgcgacag gctcaaaaaa
    14581 tggaggccgt cgggcgattg gcgggaggcg tggcgcacga cttcaacaat gtgatgaccg
    14641 tcatcctcgg ctatagcgcg gtgctgctcc aggaactgag ccagaactct tcggcccgct
    14701 actttgttca ggaaattcaa cgcgcggggg aacgctgtgc ggcactgacc ggccagttac
    14761 tggccttcag caggaaacaa atgctccacc cggtctcgct ggaccttcac cgggtgattc
    14821 gtgacctcat ggcgctgctg aagagcctga tcggggagca catctcgatc gtcctgcaac
    14881 tcgaccctct tccgctctgg gtcaaagccg acgccgtcca actcgaacag gtcctgctca
    14941 acctggccgt gaatgcccgc gacgccatgc cgcatggagg cacgctgacc atcgatacgc
    15001 gccaggtttc ccgggaagcg gtctggggat cggatcatga cacgcgcacc gcccgcacct
    15061 atgtgcgtct gcgggtgcac gatacgggaa ccggcatcga cccggccacg aaggccaaga
    15121 ttttcgagcc ctttttcacg accaaaccgc cgggccgcgg aaccggtctc ggcctctcca
    15181 cggtgtatgg catcgtgcac caaagcggcg ggaccattac ggtcgagagc gcggtgggcc
    15241 agggcaccac catgaccgtg tttctgccgg aagtcgtccc cccactgacg gccctcccgc
    15301 cggttcaatc tgaaccggcc cacacgacgg ggacagaaat catcctgctg gtggaagacg
    15361 agccggcggt gcgcctgctg acgcagcata ttcttcgcac acatggctac acggtgcacg
    15421 aagcggagga cggcttccag gccctggatc taatccgacg aaacgcgctg cacatcgacc
    15481 tgctgctgac ggatctcgtg atgcccggca tgaacggcaa agagttggcc atgcgcctcc
    15541 gcagtcactt cgcggagctg aaagtgctct acatgtccgg gtacagcgat aacccgcctg
    15601 tcacaggaga tgaagctcag ggccacacga catttttgca aaagcccttc tcgcccgagg
    15661 atttgatccg catggtgcgc gagattcttc actcagtctc gcccgcctag gagcctgtcc
    15721 gacatggcct tcgtagcgag gcgaagaagg cgcgaccaga tgcgaggccg caggcctgga
    15781 aaaaccggaa gcgtattcgc tgaaatacgt tgaggatttt ttcaggccga gaacgacgca
    15841 aatgatcgtg gatcgtttgc cgcagtcgaa cggtcaatgc cggacaggct cctagctcac
    15901 tcgacgggcg aggaaaggga cggccggttc ccggacgcgc tcaatccagc cggatgctcg
    15961 atccattgcg gcagcaagga cccggccacc atccccacgg cggcggccag caggcccacg
    16021 agctgcggcg gccagatctg atcgagcgtg acggtcagct ccgatccaca ccaggatccg
    16081 agcccgacag tgatagccag caccgcccct tgcgtcgtgg ctcttgccca gtacaatccg
    16141 gccagcaacg gtacgaaggc ggccaccagc gtgaccttgt acgcactctc gaccatctta
    16201 aaaatactgg cctctgaatt gagcgcaaac agcaggaccg tggccgcaaa cccgacgagg
    16261 acgattcgca tcacccgcaa caacccttga tcgctgatcg tgggcaccca gcccttcacg
    16321 atattttcac tgaacgcaac cgacggcgcg agtagcgtgg cggacgagca actcatgatg
    16381 gcggacagca gcgcgccgaa aaagatgatc tgcgccacga tcggagtatg ctgcacgatc
    16441 agattgggaa ggatgagttg ggaatcccgt tccagcaact gcgccacctg cgccggatcg
    16501 accaatgtgg ccgaataggc gaggaacatc ggcacaaacg cgaagaagaa atagagcgtt
    16561 ccccccagca ccgatcctcg caccgccgtc cgttcgtccc gcgccgatgt gatgcgctga
    16621 aacacatcct gctgcggaat cgatccgaac atcatcgtga cccaggcgcc gagaaacgga
    16681 atccacgcct ccatccgcgc cggcgggaaa aaatccagtt tccccgccat ggccgcatgc
    16741 gtcaccaccc gctccacccc gccggctaat ccgctgatga ccgagccgat gtagagcatg
    16801 ccgcccatga tgatggtgat ctgcacaaaa tccagaatcg ccaccgagaa catgcctccg
    16861 aacgtggtgt acgtcaggac gatgatggct cccagcacca tgcccaccga ctggctcatc
    16921 gccccgtccg tgaccacgtt gaagaccagc ccgagggcct tgatctgggc cgacacccaa
    16981 ccaaggtatg acgcgacgat gcagagggtg cagaggactt cgaccgtgcg attgtaccgg
    17041 agccggtaaa agtctccgat ggtgagaagg ttcaatcgat acagtcgacg ggcaaataac
    17101 aacccggcga gaatcaaaca gaggctcgat ccgaagggat cggccactac cgctcgcaac
    17161 ccgtctttga caaaggtggc ggagatgccg agcacagtct cggcgccgaa ccaggtcgca
    17221 aacaccgtgg cggtcaccac cggcagcggc agacagcgcc cggcgacggc gaagtctttg
    17281 gaggtatgca cgcgggtcgc cgcgtagagc ccgatcccga ccgagcaggc aagatagaga
    17341 atcacgaagg ataggagcat ggagtcattc gcgcgtcggt gagaccacac ccatcggcgg
    17401 gtgacgagcg agggggattg taccggcagc gaagcgtctg cgcaatggca ggtggagcac
    17461 gacaggcgct cacgcggtcg gttacgggcc ggcctgcgca tgcgtcatcc ccnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagacatga ggccgaacaa agccaatgcg
    17641 atagtcgcgg cggaaatcga catcctgttc atagagagtc ctccttgtga gactggtgag
    17701 tgacagcgga atccgatgcc caccacaaca gtgtctacca atgaccacac cggagcaatt
    17761 cctgaaaccg actacggaaa cttccggaac agacccctcc gtcctgagcg acaggaacag
    17821 acggtaaaaa acaacggttt cgttgacatc tcatgtcgct atggtacgaa taggccatgc
    17881 gatattcatt ctacttcgca tgggtggtgg ccgtcctttc gatctgcgtc ctgacgacgc
    17941 tggcctcgtt tccgttgctc gtgaacggcg agcgccctcc cgagccgaga atcacggcag
    18001 gagcggagaa gcttcggacg atcaagatcc accgcctgcg ctgcaatccc ttcacgccct
    18061 cgtgtcagct ttccaagcac aagccgtcac acaaagtgcc ggcttagcag ttaggaacct
    18121 ggctccgtct acgacagggg aattcgcccc ttgttcgcct ccaaccactg cgcaaaggtc
    18181 tgcagcccgg gattcagcgc ccgggcgatc gccggctcgc gtgcgccaca gaacagcgca
    18241 ttgaagtcac gtttgaactg aaacatgttg cccagatcat ccgctccggg gaacccgaat
    18301 gtccggtaca cctccggcgg caccgcgttg tagcgcaccc cctggcccag ggccttcgtc
    18361 aacgcaacgg ccatttgtga tccggtgaga tgctcccccg cgattccgac ggtcttcccc
    18421 agatacgcat cccgtttttt caacagcccc aaggcgcact tcccgatatc ctcagccgcg
    18481 ataccgggca atctcgcctc gcccatggga agcgtgaacg ccagtgtccc gtccgggccc
    18541 ttcttcggac ccatgccgaa gtggatgaga ttgtcccagt agaacgacgt cagtaagaag
    18601 gtcgtcggca caccgagttg cctgaacacc tgatccgctt cgcccttggc atcgaaatgc
    18661 ggcaccttgt acttgcccat cagtgtcggc atccggttat ccgagagcgg aacccatcga
    18721 cgcgtatctt cgagggtcga ccagatcaca tgtgggaccc ctgccgactt ggcggccttg
    18781 gccatcgcct cggcttcggc aaattccttt tcgggagaaa aatgcgccca gaaaaaggtc
    18841 acgcaaaaca ccccggcagc ccccgcaaac gcccgcttca aactctcgcc atcgtgcacg
    18901 tcggccgcga ccacctcggc gcccagtcgc gcgagctccc tggccttgtc cgaattcaca
    18961 tccctggtca atgcccgcac cgtcatgccg ctgcttggat cggccagaat cgcccgcacc
    19021 agcccgcccc cctgcatccc cgtcgccccg accaccgcaa tcacctgctt ctgtcccatt
    19081 tct
//
LOCUS       DAOQRM010000036        18687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2080_length_18687_cov_21.351814, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000036 DAOQRM010000000
VERSION     DAOQRM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18687
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2080_length_18687_cov_21.351814"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..2280
                     /locus_tag="PKK81_04965"
     CDS             325..2280
                     /locus_tag="PKK81_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV32251.1"
                     /translation="MICCCAIGAHAQDASPPATTPTAAAPVSEELELLKEEETVSIAT
                     RHEQPISQAPSNVYVITDEDIRHSGATDIPTILRRVPGLDVMQVTGADFNVSARGDNQ
                     LFANKILVMVDGRSIYIDGQGLVFWKSLPVTLPEIKRIEVLKGPASVVYGFNAFDGVI
                     NIITKSAQDMKGTTLQFGGGELGTISSSAVQAGSAGKLDYRLSIGHDQNQQWRNHDAL
                     AFRSNKFNVQTQYNFSGDATLRVAGGFIDTNRFDGPVSNVQIPRSQFNQGYTDVHYQY
                     RNFSVRGWWQGNDIPVDSATHPLLSQFVRQTNATGGSNVSFTTNTYNIEAQHSLEFGS
                     ANRLSYGVNYRYNTVDGSAVSELGREDRLGVHLQDEWKIRPSLTLVAGVRYDLHTRIN
                     GTWSPRVALVYNLTPEHTFRVSGSVAYRPPTLFESNLDLRILPTVPPLFAPIPLFGSQ
                     TLNPEQIISYEAGYQGWWMKHRLRTRLDLFFNHLSDLINFQTVGTARTLVNGGEADIY
                     GTEAGLEFLATRWLSGFANVSYQKIGQTFTGVSRRGGPDWKANVGLRGDWDNGLNGEI
                     AVHYVASATYPLIEFFTALAPVLPSPTSPTVDSYTLLNLRGGYRFWHDKAEVAVAVYN
                     ALNDKHKEHPLGDTLGSRVMGWVTLRF"
     gene            2288..4348
                     /locus_tag="PKK81_04970"
     CDS             2288..4348
                     /locus_tag="PKK81_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV32252.1"
                     /translation="MTSFELNTAWYSLSAVLLLLCLSSVAAAEPPPVIDESMTSAVSN
                     ELELLKEEETVSIASRYEQPISQAPSNVYVITDEDIRQSGAIDLPTVLRRVPGLEIMQ
                     MTGGDFNVSARGGNQPFANKMLVMVDGRSIYGDVQGTVFWKSIPVTLPEIRRIEVLKG
                     PASAMYGFNAFDGVINIITKSPEEMKGTTLQFGGGELGTISSAAVHAGTIGKFGYRLS
                     IGRDQTQQWRDRDALGFRSNKFNLQTEYALSSTSKLQVSGGLVDTNRYDGQVGEVTSN
                     SIRPSLAYANVLYEQGAFLIRAWWSGYTDNATITPNSQLVDLLSITDRNGQSTNPFSG
                     NTYNVDVQHATNLWATHRLLYGATYRHNSLSSTTIDRFSREDRLGLFIQDEWRAASSL
                     TIVAGLRYDLHTQITGTWSPRVAILYQPVEGHTFHLSGSAAYRPPTLFESHQDQRVTT
                     TLPTGVPFPPSISSTVPISGSTRLAPEQIISYEAGYQGWYWKHRLRVRADLFFNHVSD
                     LIGSRITSGGASEFVNDQGSADIYGGEAGIEFLASRWLSGFANYAYEEIGQSFSGTVK
                     RGAPRSKVSAGLRTEWDNGLSGEISYYYVGATTYPIAQTFTTLATIPTTGVIAPGDRV
                     DSYNLLNLRVGYRFWQQKAAAGYMRDAEVAVSVFNALNDEHKEHPLGDLIGSRSMGWV
                     TVRF"
     gene            4417..>5945
                     /locus_tag="PKK81_04975"
     CDS             4417..>5945
                     /locus_tag="PKK81_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV32253.1"
                     /translation="MTAITPGHSILVVDDDPDIALGLQDLLDHDGYQVNVAGTCAEAL
                     TQAHEHQYNAVLLDLGLPDGDGSSVLRTLQEQQPQLPVIILTAYTSADRTVGSLTQGA
                     FAYLTKPYNRDELRAVLRRAIGIQALAAKAEHAEHALSESEARFRSLVEAATDSIVLA
                     DHNGHILSWNQASSRLFGYTDDEVRGKPLTMLMPQRYRVAHERGLARVKDTGQSRLIG
                     RLVELEGLRKDGSEFPIELSLAMWKTDVAVFFSGIIRDITQRRKAEEILDRLRHLHEV
                     ILTQAGEGIYGLDRDGLTTFVNQTAAQLLGYDPSELIDQSMHTRLHHSRPDGSAYPAD
                     HCPIYAALHDGVVHRVPRDVFWRKDGTSIPVEYVSTPIIEKGTVAGAVVVFRDITERL
                     EAELAVEESQERFLQLAEHIREVFWITDPAKTRVLYISPGYEEVWGRSCESLYAMPGS
                     WMDAIHSDDRLRVRDAAMSRQISGAYSEEYRILRPDGSVRWIWDRAFPIRDASGTVYR
                     IA"
     assembly_gap    5946..6045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6046..7380
                     /locus_tag="PKK81_04980"
     CDS             <6046..7380
                     /locus_tag="PKK81_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV32254.1"
                     /translation="SEMGRPRGWEFRKVRKDGSVIWVRETAQAVLNDQQAPVVLIVCE
                     DITAMKEAELALHDSEEFKNQILRSSADCIKVLDLEGRLQYMNDAGQTILHIRDLNAY
                     LNRPWVDLWEGDDRNAALAALEAAKAGEVGKFVGFCPTTDGIAKWWDVQVTPMLDTRG
                     YTRRLLAISRDITDYRQVQESLRASEERLESVIRGSNDGFWDGHVLPDQPWYSPMTPV
                     WWSPRVREMLGYNEQEFPDILDSWASRLHPDDREIVFDRLTDCLKNGSSHYDVDYRLL
                     TKQGDYCWFHARAEVLDRDAHGYALRMAGSLQCITERKQAEAHLRLSEERLRRALTES
                     EVGIWDWDLQSDRLYWSPDVEVLFGLTPGSFPGTYAAYIGLIYALDRGSVLMQIEHSL
                     HDQATLNIRHRVVWTDHSIHWLAWTGRIHRNADGVATRVLGIVHAIKGHSDT"
     gene            7438..8406
                     /locus_tag="PKK81_04985"
     CDS             7438..8406
                     /locus_tag="PKK81_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV32255.1"
                     /translation="MTFPFVQSPSTTGSADRFRRSLSWTVIALVCRLFSPGLASATDV
                     VILKSSDIAAYNQAISGFKAALPNDVVLAEYDLQGDLEKGRKLTRKIRAADPALVFAV
                     GLKAAKAAQLEIVDIPVVYAMVLDPAKYGLNAPNMTGVLMEIPMERQLIMIKSLLPRL
                     KHVGTLYDPSKTATLIEDARRLLKSNGTELVPIPLNNERDVPGALRTLLPSIDALWLV
                     PDSTVLTDESLRFILNTALEERVPVVGFSREFARSGALLCLSVNYGDIGRQAGQLTRK
                     ILDGHLSLPAKPLHPDRIEMSLNRKTAKFLDIEIPRDLEEKADELY"
     gene            8406..10721
                     /locus_tag="PKK81_04990"
     CDS             8406..10721
                     /locus_tag="PKK81_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32256.1"
                     /translation="MKGPTVKGPAHYLTSPSLYPSGPGRFISLRTKFVVFFSLIIILT
                     CSGMSWYFIHSKRIAMTERVQDLGTILVKNLAHNVRYGIIIQERVILEQFIDGVMGVD
                     EVVYALITGADGQILAAKSKGKLDSPLGTVRSAERPFYPRPDIADAVIKHSGTEPTIT
                     RLRLSGADTIPLREKDSTLPISTSGIHEETFYDFAIPVMKRSESRLEALALQEEETRK
                     AANAPTTPQVFGVVQIGLTEVPMQRELAKVLRNFLLLTLGIITAGILCTNLLARRIIT
                     PLRHLAAGARKVSEGDLSTFVVPTTQDEVGQLTALFNLMTKSLQERNQAISANLETIR
                     RHVTQLTTLNQSSTAITSTLDLDRLLSIVLQLLSENLGFARMVLFLWDSERGIATVGQ
                     MLGMPPEVEEAARRLEIPVCEDGGFAAELLIRGKPLFVPSIEPVADRISPAVLPWLRQ
                     VGISSFVAAPLRSQQRILGYLGADRGTHPCSQEDLDLLVTIASHVAVAVDNARAYTEL
                     ATLTDHLERRVQERTHELQAVNERLQEHDQRRSKFVSVASHELRTPMTSIKGFVENML
                     DGLTGQLSERQEHYLQRVKHNVDRLTRIINQLLDWSRLDVGRIEIKPESLHIDEFVRD
                     VVESFQTLAAEKSIVLEVLSCEQPITVRADRDKLEQILWNLVGNAIKFTPPSGHVSVS
                     CSICDARYAQITVADTGCGIAPDELPHVFNEFSKVESAMPSSQGAQLGLFITKSLVAL
                     HGGQMCVESRLGVGTQFYFTLPLDNSPASTP"
     gene            10961..13693
                     /locus_tag="PKK81_04995"
     CDS             10961..13693
                     /locus_tag="PKK81_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV32257.1"
                     /translation="MRPPVLIVDDDPDIRESLTDMLGHEGYLVHSAASGTDALQQAKQ
                     TRYDAALLDIQLPDLNGLSVLKVLMELDASLPVIILTGNVTPENTIGSLAKGAFAYLT
                     KPYNSQELKAILRRAVSVKGLAVLAEHVEEALQASEERFRALVESATDAMVLADQSGL
                     IIWWNGAAERMFGHTKEEALGRPLTIMMPERFRAAHAAGVTRLAQGGTATIIGHTIEM
                     VGLTKTGDEFPIELSLASWRASEGMFFSGIIRNIARRKRAEESVVRLSHQNALILDSA
                     GEGIFGLDPQGCATFVNATAARMLGWSAAELIGKPLAPLFYRIGNDNALYASDPFCLR
                     AALHDGAIHRIQNETFARKDGSLFPVQYVTSPIRERDRPVSAVVVFQDISARKQQEGL
                     QQAQLSVSHILAQAGTVEEAIPQLLHVAGTMGAWDMALLWQCGPMGDKLTCQGGWIRP
                     SHRWDEFLSVCRNSEFPPGMDFPGIAWASKLPLWISDVLADSRFTRAPTASRLGIHGA
                     CIVPIRTGSIIHGVLELYTEQRRASDSHVIQILADTGMKIGQFIDRTGAAQALRAAHH
                     MTQSLLASLPGAIFLCGSDWHVQYANALAHQYFAPPGESLIGRPLCECLSLSEPALQQ
                     LMLEWSTHTSDSPHRLSERECEVAARLYRYRFFPVSTPESPQYQVGILLWDITDDKQL
                     QDELIQAEKLSSLGTMVSGMAHEINNPAQAILSMAELIQDEDDPDTIKQFAADIVGYA
                     RHVSTVVRDFSGYARAAGRDGETEIDVAERLLEAVKMVRRGPHFGHVEIATQFEAPVF
                     LRARKGEVDQVFVNLISNAIQAMEGIGCLTLATGLDGRWIQVTIADTGPGIPDSIRPR
                     IFDPFFTTKTAGKGTGLGLSIVHKIVTKYAGTITVDSTEGGGTTFRLRFPALVQ"
     gene            complement(13788..14549)
                     /locus_tag="PKK81_05000"
     CDS             complement(13788..14549)
                     /locus_tag="PKK81_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNV32258.1"
                     /translation="MSPTCTSSVTEAVERAIAALDLDRLEQEYWDQNEFLYIPEFLPR
                     DFVEATLAQQAQMLKSGLNRNYIPGHKKGGSVGYYAVMEKAPQFIDLYRSDAFRAMLN
                     RLTKVNLLLCPDNDPHSCALYYYTEAGDHIGYHYDTSYYKGARYTILMGLLDQSKQCR
                     LVCDLFKDVPGKQPVHRELATAPGDLVIFNGDKLWHAVTPLGEQEERIVLTMEYVTNP
                     DMGAFKRLYSNLKDSFAYFGLRSVFKRAYAPRSQS"
     gene            complement(14789..15511)
                     /locus_tag="PKK81_05005"
     CDS             complement(14789..15511)
                     /locus_tag="PKK81_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNV32259.1"
                     /translation="MMRIFIIVAVLLMNGIPAVAQPVKPDEPAVDLQSLQEVQEVVKK
                     IQTRYEKTKDLQASFTQKTRIEGFSTPVISTGHFYIKKPGRLRWDYIEPATEEIYVNK
                     DDVKMYVPEHKQVLVGKLTYMAASQAPLQLLQGVAKLDEEFDIEPTANKDRGAGGILL
                     VSLTPKQGRAEPERAIQKIVIEVQPKTYFLKTIALHEVSGNIATFEFSELKPNSGLKD
                     DLFDFKAPADVEIVRAPVLSRP"
     gene            complement(15508..17943)
                     /locus_tag="PKK81_05010"
     CDS             complement(15508..17943)
                     /locus_tag="PKK81_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNV32260.1"
                     /translation="MGVSTTAKRGDARSAPSRSSHVKREVIGVLLIAAGLLILLSLVS
                     FVPGDAKSIAASGAAGNQPKNMIGSVGALSAAACFFMVGGAAYLFPILLGLLGARCFT
                     PIPLTMRIRNAGSGLAAMVFLSALLHLEVTAVPTISSGWVNRGLAGGIIGQVLADGVR
                     SYFATTGAHIVILAGLMVALLFTVPLSLTALLQRVPDWWVAARERMAGLVPEWPTKQE
                     EAQPKRVREKKSRASREVEEQDFDREVQVVAEAVEQIPVPVIPPKIQPPMKVEKRAVS
                     ADEPAAAVATSPSVSDGYVLPDPQELLSDPSGPLARLSDDELKLQSEILTKALKSFAI
                     EGRVTEVRPGPVVTMYEFEPAPGTKVARIVNLADDLALALKAISLRIVAPLPGKSVVG
                     IEVPNPHREMVSMKEVVTSDAFSRSRSKLGLALGKDIFGGAVCADLRTMPHLLVAGAT
                     GAGKSVGLNTMLLSILFNARPDEVKLLLIDPKMLEFQSYDGIPHLLRPVITDPKSAAR
                     GLGWVVQEMERRYKLLADAGVRSIDAYNRRISEVQGAVSDVWQSGKPEQVELTFLSEE
                     ERLSKGEDAEPAGDNGPTDSVKPSPPEPLPYIMVMIDELADLMMVAPKDVEDKIARLA
                     QMARASGIHLVLATQRPSVDVLTGLIKANFPARIAFQVSSKTDSRTILDANGAEALLG
                     RGDMLYLASGTGKLMRIHGSYVSDDDVRRVVEFVKKQALPSYCRELQSLKIEEAEEEQ
                     AKDEVYEQAKDLVLSTGQASASLIQRRLRVGYPRAARMIEQMEAEGVVGAAGRDGRRE
                     VLGRRGPVGGDEA"
     gene            complement(18043..>18687)
                     /gene="rsmA"
                     /locus_tag="PKK81_05015"
     CDS             complement(18043..>18687)
                     /gene="rsmA"
                     /locus_tag="PKK81_05015"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNV32261.1"
                     /translation="AEAGRVIAVEIDPQLQPHLQESLGHCRNLDLRIGDALAFPFETL
                     PLRTVVVANLPYYVSTPILFALLDARARLDRLVLMLQTEVALRLAAKPNSEDYGVLSV
                     LTQEAAEVELAFRVSANCFRPRPTVGSAVVHLRINTQEGFEPVRHERFRRLVRASFAH
                     RRKTLVNSLRDEGYPSEQIARATAEAGVPPQARAEMLTLDDYRTLARAFDRDPV"
BASE COUNT         3931 a         5853 c         5169 g         3634 t
ORIGIN      
        1 acctcgctcc ttgcgtagac ggccgaaatc gcgtgaatcc cccgataatt cacgcgtctg
       61 ctttccgcat accgagaatc ccataagcat gcattttgcg tgagagtact tctaatcata
      121 tggtcctcgt gcttgtcttc tgtccaacca tgcccgacac cgccgggccg ggatccacgg
      181 tatgccatgc gccctctgat gcaccccccc ggcaccgtct cctcccccct atctccctct
      241 ccgccggcta agcgacaccc tgtggcgaca ggaccgacca ggtatcggtt acgacactct
      301 gcccctccac tatgtggtgg actcttgatc tgctgctgtg ccatcggcgc tcatgctcag
      361 gacgcatcgc ctcccgcgac gactccaaca gcagcggcac cggtcagcga agagctcgaa
      421 ctgctcaaag aggaagaaac cgtcagcatc gccactcgcc atgagcaacc catctcgcaa
      481 gcaccctcca acgtctacgt catcaccgac gaggacattc ggcactcggg cgcgaccgac
      541 attcccacta ttctccgccg cgtacccggg ctggatgtca tgcaggtcac cggggcggac
      601 ttcaatgtca gcgcacgagg cgacaatcaa ctcttcgcca acaaaattct cgtgatggtg
      661 gacggtcgct cgatctacat cgacggccag ggcctcgtgt tctggaaatc tcttccggtc
      721 acccttccgg agattaaacg catcgaggta ctgaaaggac ccgcctctgt tgtttacggc
      781 ttcaatgcct tcgacggggt gatcaacatc atcacgaagt ccgctcagga catgaagggc
      841 acgacgttgc agttcggcgg cggggaactc ggcaccatct ccagttccgc tgtccaggct
      901 ggatcagcgg gcaaacttga ctaccgtctc tccatcggcc atgaccagaa tcaacaatgg
      961 cgcaaccatg acgcactcgc cttccggtcg aacaagttca acgtgcagac gcagtacaac
     1021 ttctccggtg acgccacctt gcgcgttgcc ggagggttca tcgataccaa tcgcttcgac
     1081 ggccccgttt caaacgtcca gattcctcgt tcccaattca atcaaggata tacggacgtg
     1141 cattaccagt accgcaattt ctccgtgcgc ggctggtggc agggcaacga catccccgtc
     1201 gactccgcca cgcacccgct cctctcgcaa tttgtccggc agacgaacgc gacgggcggg
     1261 tccaatgtgt ctttcaccac gaacacctat aacatagagg cccaacacag cctcgaattc
     1321 ggctcggcca accggctgag ttatggcgtc aattatcgtt acaacacagt cgacggcagt
     1381 gccgtctcag aattagggcg agaagatcgt ctcggggtcc atctccagga cgagtggaag
     1441 atacgtccat ctctcaccct cgtggccgga gtccgctacg acctgcacac cagaatcaac
     1501 ggcacctgga gtccgcgtgt cgccctcgtc tacaacctga cgccggagca tacgtttcgc
     1561 gtcagcggat ctgtcgccta ccgccccccg accctattcg aatcgaatct tgaccttcgc
     1621 atcttgccga ccgttccacc gctgttcgca cccatcccgc tgtttggctc acagacactc
     1681 aatcctgagc agatcatttc ctacgaagcg ggataccagg gctggtggat gaaacaccgg
     1741 ctccggacca ggctcgacct cttcttcaat cacctctccg acttgatcaa cttccaaacc
     1801 gtcggcaccg cgcgcacgct cgtcaacggc ggcgaggcgg atatctacgg aaccgaagca
     1861 ggcttggagt tcctcgccac tcgctggctc agcggctttg cgaatgtgtc ctaccagaag
     1921 atcggacaaa ccttcaccgg cgtctcacgg cgcggcggcc cggactggaa agcgaacgtg
     1981 ggcctccgcg gcgactggga caacggcctc aacggagaaa tcgcagtcca ttacgttgca
     2041 agcgccactt accccttgat cgaatttttt accgcgctcg caccggtgct gcctagcccc
     2101 accagtccga cggtcgatag ttacaccctg ttgaacctgc gtggcggata ccgattctgg
     2161 catgacaagg ctgaagtggc cgtggccgtc tacaatgcgc tcaacgacaa acacaaggaa
     2221 catcccttgg gcgatacgct cggaagccgc gtgatgggtt gggtcacctt gaggttttaa
     2281 attgaacgtg acgtcttttg aattgaacac agcctggtat tcgttgtcgg cagtgctgtt
     2341 gctcctgtgc ctcagcagcg ttgcggctgc cgaaccgccg ccggtcatcg acgagtccat
     2401 gacttccgcc gtcagcaatg aactggaact cttgaaagaa gaagaaaccg tgagcattgc
     2461 atctcgttat gagcaaccga tctcacaggc gccatcgaac gtgtatgtga tcacggacga
     2521 agatatccgg caatccggcg ctattgattt gccgactgtc cttcgccgcg ttccgggcct
     2581 ggaaatcatg cagatgacag gcggcgactt caatgtgagt gccaggggag gaaaccagcc
     2641 cttcgcaaac aagatgctcg tcatggtgga tggacgatcc atctacggcg atgtccaggg
     2701 gaccgtcttc tggaaatcca ttccggtcac cctacctgaa attagacgca tcgaagtcct
     2761 caagggtccc gcctcggcca tgtatgggtt caatgctttc gacggggtca tcaacatcat
     2821 caccaaatcg cctgaagaga tgaaaggcac gaccctgcaa ttcggaggag gtgaactcgg
     2881 cacgatcagc agcgccgcgg tccatgccgg gaccatcggg aagttcggtt accgcctctc
     2941 catcggtcgc gatcagacac aacagtggcg ggatcgtgat gctctggggt ttcgatcgaa
     3001 taagttcaat ctacagaccg agtacgcctt gtcgtcgact tccaaactgc aggtatcagg
     3061 cgggctggtc gacaccaatc ggtacgacgg gcaggtcggg gaagtcacca gcaattcgat
     3121 ccggccatcg ctcgcttacg ccaatgtcct ctatgagcag ggcgcctttc tcattcgagc
     3181 ctggtggtca ggatacacgg acaacgcaac gattactccc aactctcaat tggtcgatct
     3241 cctgagcatt accgaccgca acggacaatc gaccaatccc ttctccggga atacctataa
     3301 cgtcgatgtg caacatgcaa ccaatctgtg ggcgactcac cggctgctct atggcgccac
     3361 gtatcgccac aactcattgt cgagcacgac gatcgaccgg ttcagtcgtg aagaccgcct
     3421 ggggctcttc atccaggatg aatggcgcgc cgcatcttcg ctgacgatcg tcgccgggct
     3481 gcgctacgat ctccacacac aaatcaccgg gacttggagc ccgcgtgtgg ccatcctcta
     3541 ccagcccgtt gagggccaca cctttcacct ctccggatcg gctgcctacc ggcccccgac
     3601 cctcttcgaa tcccatcagg accaacgcgt gaccacgacc cttccgactg gtgtgccgtt
     3661 tccgccctca atctcgtcga cggttccgat cagcggctcg acccggctcg cccctgaaca
     3721 gatcatttcg tatgaggccg gctatcaggg ctggtattgg aaacaccgct tgagggtgcg
     3781 ggcggaccta tttttcaacc atgtctccga tctgatcggc agccgaatca cctccggcgg
     3841 cgcttccgaa ttcgtcaacg accagggctc tgcggacatc tatggcggcg aggccggaat
     3901 tgaatttctc gccagccgct ggctcagcgg atttgccaac tatgcctatg aggaaatcgg
     3961 ccagtcgttc agcgggacgg tcaaacgggg tgccccgcga tcgaaagtca gcgcgggtct
     4021 gaggaccgaa tgggacaacg gcctgagcgg ggaaatcagt tactactatg tcggcgcaac
     4081 cacgtatccc attgcacaaa ccttcacgac attggcgact attccgacga ccggcgtcat
     4141 cgcgccgggg gatcgcgtcg acagttacaa cctgctcaat ctccgcgtcg gatatcgatt
     4201 ctggcaacaa aaggccgccg ccgggtacat gcgcgacgcg gaagtcgctg tctccgtctt
     4261 caatgccttg aacgacgaac acaaagaaca tccgttgggc gacctcatcg gcagtcggtc
     4321 gatgggatgg gtcactgtga gattttgacg gaatcatcgg catgctcgga gcaaggtttc
     4381 aggccatgag aactccggag tgacagcgag gccgctatga cagccattac acccggccat
     4441 tcgatcctcg tcgtggacga cgatccagac atcgccctcg ggctacaaga cttactcgac
     4501 cacgacggct atcaggtgaa cgtcgccggc acctgcgcag aagcgctcac gcaagcgcat
     4561 gagcatcagt acaatgccgt cttgctggat ttgggcttac cggacggaga cggctcctcg
     4621 gtcctgcgaa cattgcaaga acaacaaccg cagcttccgg tcatcatcct cacggcctac
     4681 acaagcgccg accgtaccgt cggttcactc acacagggcg cattcgccta cctcaccaaa
     4741 ccctataacc gggacgaatt gcgggccgtc ctgcgccggg cgatcggcat ccaggcgctc
     4801 gccgccaaag ccgagcacgc cgaacatgcg ctgagcgaaa gcgaagcacg atttcgatct
     4861 ctggtcgagg ccgccaccga ctccatcgtg ctggcggacc acaacggtca tattctctcc
     4921 tggaatcaag cctcctcccg cttgttcggg tataccgatg acgaagtccg agggaaaccc
     4981 ctgaccatgt tgatgccgca acggtatcgc gtcgctcacg agcgagggct cgcccgcgtg
     5041 aaggacaccg gtcagtcccg ccttatcggt cgtctggtcg agctggaggg cctgcgcaag
     5101 gacggcagcg agttcccgat cgagctgtcg ttggccatgt ggaaaaccga tgtcgccgtc
     5161 tttttcagcg gcatcattcg cgacattact caacggcgca aggcagaaga gattctcgac
     5221 cgccttcgcc atctgcacga agtcatcctc acgcaagcag gggaaggcat ctacggactg
     5281 gatcgcgacg gcctgaccac cttcgtgaat cagaccgccg ctcaattgct cggatatgac
     5341 ccatccgaac tcatcgacca atcgatgcac acacggctcc accattcccg gccggacggc
     5401 agcgcctatc cggccgatca ctgcccgatc tatgcggccc ttcacgacgg cgtagtccac
     5461 cgtgtgccgc gcgatgtctt ctggagaaag gacggcacca gtataccggt ggaatatgtg
     5521 agcaccccga tcattgagaa gggcacggtg gcaggggccg tggtcgtctt ccgtgacatc
     5581 actgagcgcc tggaagccga actcgccgtc gaagaaagcc aggagcgatt cctccagctg
     5641 gcggagcaca tccgggaagt gttctggatc accgatccgg ccaagaccag agtgctctat
     5701 atcagtcccg ggtacgaaga agtctggggg cgttcctgcg aaagcctcta cgcgatgccg
     5761 ggctcgtgga tggacgccat ccattcagac gaccggctac gggtccggga cgcggccatg
     5821 agccggcaaa tttcgggagc gtacagtgag gaatatcgca tcctccgccc cgatggatcg
     5881 gtgcgatgga tctgggatcg cgcattcccg attcgtgacg cgtcggggac ggtctatcgc
     5941 atcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntccga aatgggccgg
     6061 cccagaggtt gggagtttcg caaggttcgg aaggatggca gcgtcatctg ggtgcgagaa
     6121 accgcccagg ctgtgctgaa cgaccaacag gctccggttg tgctgatcgt gtgcgaggac
     6181 atcaccgcca tgaaagaggc ggaactcgcc ctccacgaca gcgaagaatt taagaatcaa
     6241 atcttacgta gcagcgccga ctgcatcaaa gtgttggacc tggaaggccg gctccaatac
     6301 atgaacgacg ccgggcagac gattttacat atcagggatc tcaatgccta cctcaataga
     6361 ccttgggtgg atctttggga gggggatgat cggaatgccg ccctggctgc attggaagcg
     6421 gccaaggccg gcgaggtcgg aaagttcgtc ggcttctgcc ccaccaccga cggaatcgca
     6481 aagtggtggg acgtacaggt cacccccatg ctcgacacgc ggggctatac ccggcgcctc
     6541 ctggctattt cccgtgatat caccgactac agacaggttc aggaatcgct gcgcgccagc
     6601 gaagagcgac tcgaatcggt catccgcgga tcgaacgatg gattctggga cggccatgtg
     6661 cttcccgacc agccctggta ctcgccgatg acgccggtct ggtggtcgcc gcgcgtgcgt
     6721 gagatgctgg gatataacga gcaagaattt cccgatatcc tcgacagttg ggcctcccga
     6781 ctgcacccgg atgatcgcga aatcgtattc gatcggctca cggactgcct caaaaatggc
     6841 agctctcatt acgacgtcga ctaccggttg ctgaccaagc agggtgacta ctgctggttt
     6901 cacgcgcggg ccgaagtgtt ggatcgggat gcgcacgggt atgcgctccg catggccggc
     6961 tcgctgcaat gcatcacgga gcgcaagcag gcagaagcgc acctacgcct cagcgaagaa
     7021 cggttgcgca gggccctgac cgaatcggaa gtcggcatct gggattggga tctgcagtcc
     7081 gaccgtctct actggtcacc cgacgtcgag gtcttgttcg gtctgacccc cgggtctttt
     7141 cccggcacct atgccgctta tatcggcctg atttatgcgc tggatcgcgg gtcggtcctg
     7201 atgcagatcg aacacagttt gcacgaccag gccactctca acatccggca tcgcgtcgtg
     7261 tggaccgatc acagcatcca ttggctggcc tggaccggcc gtattcatcg caacgccgac
     7321 ggggtcgcca cacgcgtcct cggtatcgtt cacgcgataa aggggcactc ggacacgtag
     7381 acagggggca ttcggcaatc gctcacgggg ccggctggat cgacgaggat acagactgtg
     7441 acgttcccat tcgtacaatc accctccacc acgggctccg ccgatcggtt tcgacggagc
     7501 ctgagttgga ccgtcatcgc cctcgtctgt cggctcttca gtcccgggct ggcctcggcc
     7561 acggacgtcg tcattctcaa gtcgtcggac atcgccgcct ataaccaagc catcagcggc
     7621 ttcaaagccg cccttccaaa cgacgtcgtg ctcgccgaat atgatttaca aggtgatttg
     7681 gagaaaggac ggaaactgac ccgcaagatc cgtgcagccg acccggcact ggtgttcgcc
     7741 gtcggcttga aggcggcgaa ggcggcacaa ctcgagatcg tcgacattcc agtggtctat
     7801 gccatggtgc tggatccggc caagtacggg cttaacgccc cgaatatgac gggcgtgctc
     7861 atggaaattc caatggagcg gcagctcatc atgattaaat ccctactgcc tcgtctcaaa
     7921 cacgtcggga ccttgtacga tccatccaaa accgcgacgc tgatcgaaga cgcgcggcga
     7981 ctgctcaaat ccaacgggac ggaacttgtc ccgatcccgc tcaacaacga gcgggatgtg
     8041 ccgggcgctc tgcgcacctt gctgccgtcg atcgacgcat tgtggctcgt ccctgactcc
     8101 accgtgctga ccgatgaatc gctgcggttt atcttgaata ccgcattgga ggaacgcgtg
     8161 ccggtggtcg gattttcccg cgagttcgcc cgaagcgggg ccctgctgtg cctgtcggtc
     8221 aactatggcg atatcggacg acaagcgggg caactcaccc gcaagatcct ggacggtcac
     8281 ctctcactcc ccgcaaaacc tttacacccg gaccgtatcg aaatgagtct gaatcgtaaa
     8341 accgccaagt tcctcgacat cgagatcccc agagacctcg aggaaaaggc cgacgagctc
     8401 tattgatgaa aggacccacc gtgaaaggcc ccgcgcacta cctcacctca ccctcgttgt
     8461 acccgagcgg cccgggacga tttatcagtc tcaggacgaa gttcgtcgta tttttcagct
     8521 tgatcattat tctgacctgc tcgggaatga gttggtactt cattcacagt aaacgcatcg
     8581 ccatgaccga acgagtgcaa gatctcggaa ccattctggt caagaatctc gcccacaacg
     8641 tacgatacgg catcatcatc caggaacgag tcatcctgga gcagttcatc gacggtgtca
     8701 tgggagtcga cgaggtggtc tatgcgctga tcaccggcgc agacggccag atcctcgccg
     8761 caaagagcaa aggcaaactc gacagcccgc tcgggaccgt ccgatcggca gaacgacctt
     8821 tttatccgcg tcctgacatt gcggacgcgg tgatcaagca ttcaggcacg gagccgacca
     8881 tcacgcgcct caggctcagc ggtgcggaca caatccctct gcgggagaag gactcgaccc
     8941 ttccaatttc gacatcgggc attcacgaag agacgttcta cgattttgcc atcccggtga
     9001 tgaagcgctc ggaatcccgc ctggaagcgc tggcgctcca ggaggaagaa acacgcaaag
     9061 cggcgaacgc accgaccacc cctcaggtct tcggcgtcgt ccaaatcggc ttgaccgagg
     9121 tgccgatgca acgagaactg gcgaaagttc tgcgaaactt tctcctgctc accctcggca
     9181 tcatcacggc cggcattctc tgcacgaatc tgctggcccg ccggatcatc actcccttgc
     9241 gccatctcgc cgcaggcgca cgcaaagtca gcgagggcga tctttccacc tttgtcgttc
     9301 cgacgaccca ggacgaagtc ggccaactga cggcgctgtt caatttgatg accaaatctc
     9361 ttcaagaacg caatcaggcc atctccgcca accttgaaac catccggcga catgtcacgc
     9421 aactgaccac cttgaatcag tccagcacag ccatcacctc cacgctcgac cttgaccggc
     9481 tcctctcaat tgtattgcag ctgctcagtg aaaatttggg attcgcccgg atggtcctgt
     9541 ttttatggga tagcgagcga ggcatcgcga cggtggggca aatgctgggc atgcccccgg
     9601 aagtggaaga agccgctcgg cggcttgaga ttcccgtgtg cgaagacggc gggtttgccg
     9661 ccgaacttct gatacgtggc aagcccctgt tcgttccgag cattgagccg gtcgccgatc
     9721 gcatctcccc cgcagtgctt ccctggcttc gtcaggtggg aatctcctcc ttcgtcgctg
     9781 cgcctcttcg gagtcaacag cggattctcg gctacctggg cgccgaccga ggcacccatc
     9841 cctgcagcca ggaagatctc gatcttctgg tcaccattgc cagtcacgtg gcggttgccg
     9901 tggacaatgc gcgcgcctat accgaactgg ccacgttgac ggaccatctc gaacgacgcg
     9961 ttcaggaacg aacgcatgaa ttgcaagcgg tgaacgagcg cctgcaggag cacgaccaac
    10021 ggcgctcgaa atttgtgtcg gtcgcgtcgc atgaattacg cacgccgatg acctccatca
    10081 aaggtttcgt cgagaacatg ctggacggcc tcaccggtca actctcagag cggcaagagc
    10141 actatctcca gcgggtgaaa cacaatgtgg atcgactcac acgaatcatc aatcaactgc
    10201 tggactggtc tcgcctggat gtcggccgga tcgagatcaa gcccgaatca ctgcacatcg
    10261 atgagttcgt ccgggatgtg gtggagagct ttcagacact cgccgccgaa aaatccatcg
    10321 tcctcgaagt cctgtcctgc gaacaaccga tcaccgtgcg tgccgaccgc gacaagctgg
    10381 agcaaattct ttggaatctg gtcggcaacg ccatcaaatt cactccacct tcaggacatg
    10441 tctcagtgag ctgcagcatc tgcgacgcga ggtacgccca gattacggtg gccgacaccg
    10501 gctgcggaat tgcccccgac gagttacccc atgtctttaa tgagttctcc aaggtcgaat
    10561 cagccatgcc ctcttcacaa ggggcgcaac tcgggctctt catcacgaag agtctggtgg
    10621 ccctgcacgg cggacagatg tgtgtggaaa gccggctcgg tgtcggaact cagttctact
    10681 tcaccttgcc tctcgacaac tcacccgcat cgacgccgta gcaggctatg tggtagtgcg
    10741 acagcgtccc ctcggctgta tccgatccga taaaccccgc atttatttga acactcgcag
    10801 gcagcgccac acggcgcaca cggcttccct ctctcgctgt tcctcacaga atcaatgggt
    10861 tcccgctctg ttggctcacg cttcatcggc gcaccggcgg cataggtatt gcgctcacag
    10921 caaatcgtga agacttctca caatccggaa cagtcgcacc atgcgccctc ccgttctcat
    10981 tgtcgatgac gatccggata tccgggaatc tctcacggac atgttaggtc acgagggata
    11041 cctcgtacac agcgccgcct ccggaaccga cgcccttcaa caagccaaac agacgcgcta
    11101 cgacgcagcg ctcctcgaca tccaattgcc cgacctcaac ggactctcgg tcttgaaagt
    11161 gctcatggaa ttagatgcga gtctccccgt catcatcctg accggaaacg tcacgcctga
    11221 aaacacgatc ggctctctgg ccaaaggggc cttcgcgtat ctgaccaagc cctataattc
    11281 ccaagaactc aaagccatcc ttcgccgggc cgtctcggtg aaagggctgg ccgtcctggc
    11341 cgagcatgta gaggaggcgc tgcaggccag cgaagaacgc ttccgggcgt tggtggagtc
    11401 ggcgaccgac gccatggtgc tcgccgacca gagcggcctc atcatctggt ggaacggcgc
    11461 cgccgagcga atgttcggtc acaccaaaga agaggccctt gggcgcccgc tgaccatcat
    11521 gatgccggaa cggtttcgcg ccgcgcacgc ggccggcgtc acacgtctcg cccagggtgg
    11581 aacagccacg atcatcgggc ataccatcga aatggtcggc ttgacaaaaa ccggagacga
    11641 attcccgatt gagctctcgc tggcatcctg gcgtgcgagt gaagggatgt ttttcagcgg
    11701 catcattcgc aatattgcgc ggcgcaaacg cgccgaagag tctgtcgtca ggttgagcca
    11761 tcagaacgcg ctgattctcg acagcgccgg ggaaggcatc ttcggactag acccgcaagg
    11821 atgcgccacc ttcgtcaacg caactgcggc gcggatgctc ggatggagcg cggcagaact
    11881 gatcggcaag ccgttggcac cgctcttcta tcgaatcggc aacgacaatg cgctgtacgc
    11941 ctcagaccct ttctgtctcc gcgcagcgct tcatgacggc gccattcacc gcatccagaa
    12001 cgagaccttc gccaggaaag acggctcgct gtttcccgtc caatacgtca ccagtcccat
    12061 ccgtgagcga gaccggccgg tcagcgcagt cgtggtcttc caggacatca gcgctcgcaa
    12121 acagcaggag ggcctacagc aggcacaact ctccgtcagc catatcctcg cgcaagccgg
    12181 caccgtcgaa gaagccatcc cgcaacttct ccacgtcgca ggcacaatgg gtgcgtggga
    12241 tatggcgctc ttgtggcaat gcgggccaat gggagacaag ctgacttgtc agggaggctg
    12301 gatccgtccc tcacatcgat gggatgaatt tctctcggtc tgtcgcaaca gtgagttccc
    12361 gccggggatg gatttccctg gcatcgcctg ggcctcaaaa ctcccgctct ggatatcgga
    12421 cgtcctggcg gattcccgat tcacccgcgc accgaccgcc tctcgcctgg gtattcacgg
    12481 ggcctgcatc gtccccatcc ggaccggcag catcatccac ggagtgcttg agttgtatac
    12541 cgaacagcgg cgggcctccg acagccacgt gattcagatc ctcgccgaca cgggcatgaa
    12601 gatcggacag ttcatcgatc gcacaggagc cgcgcaggca ttacgtgcgg cgcatcacat
    12661 gactcagagc ctgctggcca gtctgcccgg cgccattttc ctgtgcggaa gcgactggca
    12721 cgttcagtat gccaatgcgc tggcccatca gtattttgcg cctcccggcg aatcgctgat
    12781 cggccgtccg ctgtgcgaat gtctttccct cagcgagccc gcgctccaac agctgatgct
    12841 ggaatggagc acgcacacgt cagactcgcc tcatcggctc tctgaacgtg agtgcgaagt
    12901 cgccgcacgg ttgtatcgct atcggttttt tccggtctcg accccggaat ccccgcagta
    12961 ccaagtgggc atcctgttgt gggatatcac cgacgacaaa caactccagg atgaactgat
    13021 ccaggctgaa aaactctcca gccttggcac catggtgtcc ggtatggcgc acgaaatcaa
    13081 caacccggcc caggctatcc tcagcatggc cgagttgatc caggatgagg acgatccgga
    13141 cacgataaaa cagtttgccg ccgatattgt gggctacgcc cgtcacgtct ccaccgttgt
    13201 gcgggacttc tccggctatg cccgcgcggc cggcagggat ggagagacgg aaatcgatgt
    13261 ggcagaacgt ttgctcgaag cggtcaagat ggtccggcga gggcctcatt tcggccacgt
    13321 ggagatcgcc acacagttcg aagcccctgt gtttcttcgc gcgcgaaagg gagaagtcga
    13381 tcaggtgttt gtcaacttga tcagcaacgc tatccaggcc atggagggga tcggctgctt
    13441 gacgttagcg accggtctgg acggcagatg gatccaggtg accattgccg ataccggtcc
    13501 gggcatcccg gattccatcc gcccgaggat tttcgatccg ttcttcacca ccaaaacagc
    13561 cggcaaaggg acgggactcg gactcagcat cgtgcacaag atcgtgacca aatatgccgg
    13621 aaccatcacg gtggacagca ccgaaggcgg aggcaccacc ttccggttac ggtttccggc
    13681 actcgttcag taagagaaac cgtcacgatg agggtatgat tcgattgtag cctgacgaga
    13741 agagcctcgc gaggagtcag ccggcaaact tgaaaccgcg acagatatca ggactgtgag
    13801 cgaggagcgt acgcccgctt aaataccgag cgcaatccga aataggcgaa tgaatctttc
    13861 aggttggagt acaggcgctt gaacgcgccc atgtcgggat tcgtcacata ctccatggtc
    13921 agtacgatcc gctcttcctg ctctccgagc ggcgtgacgg catgccagag tttgtcgcca
    13981 ttgaagatca ccagatcgcc tggcgcagtc gccaattcac ggtgcaccgg ttgtttcccc
    14041 ggcacatctt tgaaaagatc gcagactaag cggcactgct tggactgatc gagtaagccc
    14101 atcagaatgg tgtaccgggc gcctttgtag taggacgtat cgtagtgata cccgatgtga
    14161 tcgccggctt ccgtgtagta atagagcgca caggaatggg gatcgttgtc gggacacagc
    14221 agcaaattca ccttggtcaa ccgattgagc atcgcccgga aggcgtccga gcgatacagg
    14281 tcgatgaact gcggcgcttt ctccatcacg gcataatagc cgacactgcc gcccttctta
    14341 tgcccgggaa tataattgcg gttcaaccct gacttgagca tctgggcttg ctgagccagg
    14401 gtggcctcca caaaatcgcg cggcaaaaac tctgggatat acaggaattc gttctgatcc
    14461 caatattcct gctcaagccg gtcgagatcc agcgccgcaa tcgcccgctc caccgcctcg
    14521 gtcacgctgc ttgtacaggt tgggctcata ccttcgcctt tcgtgaagcg tcgttcgtat
    14581 ctcgtccgga gaacgagcgt atggcgcata gctggcagca gcagtgggcg aagcagagcg
    14641 aatggccaat agccgacgac agggagcccg aaatcagagg cgcagagctt ctcataccat
    14701 cctcagccat atgctataag ccatgagcta tacgctccga tctctcacga gatacgcttc
    14761 acgattcacg cgtcacggat gtcgagagct agggtctgct cagcaccggc gcccgtacga
    14821 tttctacatc ggccggggcc ttgaaatcaa ataggtcgtc cttcagtccg ctgttcggct
    14881 tcaactctga aaactcaaag gtcgcgatat tgccgctgac ctcatgcagg gcgatcgtct
    14941 tgaggaagta cgtcttcggc tgtacttcga tcacaatctt ctgaatcgcg cgctccggtt
    15001 cggcccgtcc ctgcttcgga gtcaaagaga ccagcagaat gccccctgcg ccacggtcct
    15061 tgttcgccgt gggttcgatg tcgaactctt catccaattt ggccacccct tgcagcaatt
    15121 gcagcggagc ctgggaagcg gccatatagg tgagcttgcc caccagcacc tgcttgtgct
    15181 ccggcacata cattttgacg tcgtctttgt tgacgtaaat ttcttccgta gccggttcga
    15241 tataatccca tcggagacgc ccgggcttct tgatatagaa atgtccggtc gaaatcacgg
    15301 gcgtcgaaaa tccttcgatc ctggtttttt gcgtgaacga ggcctgcaag tccttggtct
    15361 tttcatagcg ggtctggatc tttttcacga cctcttggac ctcctgcaga gactggaggt
    15421 ccaccgccgg ctcatcgggc ttcaccggtt gagccactgc cggaattcca ttcatgagca
    15481 gcacagcaac gattatgaat atccgcatca tgcctcatct ccaccgaccg gcccacgacg
    15541 gccgaggacc tctcgacgcc cgtcccggcc tgctgctccg accacacctt ccgcttccat
    15601 ctgttcgatc atacgggccg cccggggata cccgacgcgg agacgccgtt gaatcaggga
    15661 agcagaagcc tggccggtgg acaataccag atccttggcc tgttcgtaga cttcgtcctt
    15721 ggcttgctcc tcttccgcct cttcgatctt gagcgattgg agttcccggc agtacgaggg
    15781 caacgcctgc ttcttgacga attcgaccac ccgccgcaca tcatcgtccg acacatacga
    15841 cccgtgaatc cgcatcagct tcccggtccc cgaggcgaga tacaacatat cgccgcgacc
    15901 aagcagcgcc tcggcgccgt tggcatccag aatcgtgcgc gagtcggtct tggacgagac
    15961 ttgaaacgcg atacgcgccg ggaaattggc cttgatgagg ccggtcaaca catcgaccga
    16021 tggcctttgg gtcgccaaca cgaggtgaat cccggaagcg cgcgccatct gggccagacg
    16081 cgcaatcttg tcctccacat ctttcggggc taccatcatc aagtccgcca gctcgtcgat
    16141 catgaccatg atgtagggca ggggctccgg cggactcggc ttgaccgaat cagtcggccc
    16201 attgtcgccg gcgggctccg catcttctcc cttggagaga cgctcctctt cggacagaaa
    16261 cgtcagttcg acctgctcag gcttgccgga ttgccagaca tccgacacgg ccccttgcac
    16321 ctcggaaatc cgccggttgt aggcatcgat gctgcgcacc cccgcatcag ccagcagctt
    16381 gtagcgccgc tccatttcct ggaccaccca ccccaacccg cgcgccgccg atttgggatc
    16441 ggtaatgacg ggacggagca aatgcggaat gccatcgtaa ctttggaact ccagcatttt
    16501 cggatcgatg agcagcagtt ttacttcgtc cggacgggcg ttgaacagaa tgctcaacaa
    16561 catcgtgttc aaccccacgc tcttgccggc tccggtcgcg ccggcaacca gcaggtgagg
    16621 catcgttctc aggtccgcac agaccgcacc gccgaagata tctttcccga gcgcgagccc
    16681 caatttggag cgtgagcgcg aaaaggcgtc gctggtgacg acttccttca tggacaccat
    16741 ttcgcgatga ggattcggca cttcaatccc gacgaccgac ttcccgggca acggcgccac
    16801 gatacgcaag ctgatggctt tgagtgccag ggccaggtca tcggcaaggt tgacgatgcg
    16861 cgcgaccttc gtccccggcg cgggctcgaa ctcgtacatc gtcacgaccg gacccggtcg
    16921 cacctccgtc acccgccctt cgatcgcaaa actcttcagc gccttggtca ggatttcgga
    16981 ttgcagtttc aactcatcgt cgctgagacg ggcaagcggg ccggagggat cgctcagcag
    17041 ttcctgggga tcaggcagga catagccgtc ggatacggag ggactggtcg caactgctgc
    17101 tgccggctcg tccgccgaca ccgcgcgttt ttcgaccttc atcgggggct gaatcttagg
    17161 gggaatgacg ggaacgggaa tctgctccac cgcttcagcc accacctgca cttcgcggtc
    17221 aaagtcctgt tcctcaacct cgcgcgaagc tcgcgacttc ttctcacgga ctcgcttggg
    17281 ctgcgcctcc tcctgcttcg tcggccactc cggaaccagc ccggccatcc gctcgcgcgc
    17341 ggccacccac cagtcgggaa cccgctgcag cagcgctgtc agcgacagcg gcaccgtaaa
    17401 caggagcgcc accatcaagc cggcgaggat cacgatatgc gcgccggtcg tggcaaagta
    17461 acttcggacc ccgtcggcca acacctgccc gataatcccg ccggccagac ctcgattgac
    17521 ccagccgctg gaaatcgtgg gcaccgccgt cacttccaaa tgcagcaggg cactcaagaa
    17581 caccatggcc gccagaccgc tgccggcgtt tctgattcgc atagtgagcg ggatcggcgt
    17641 gaagcaacgc gcgccgagca gtccgagaag aatgggaaag aggtaggcgg cgccgccgac
    17701 catgaaaaaa caagcggccg ccgacagcgc gcccaccgac ccgatcatgt ttttgggctg
    17761 attgccggct gctccggatg ccgcaatcga tttggcgtcg ccgggaacga acgacaccag
    17821 actgagcagg atcaggagac cggctgcgat caggagaacc ccgatcactt cgcgcttcac
    17881 atgagaggaa cgggaagggg ctgagcgggc gtcaccgcgc tttgccgtgg tggatacacc
    17941 catgcggcgg atcgtagcac agggaaaacg ccttttcaaa caaacgggga atgttgcgca
    18001 agccgatgaa gccgcgctta ctcacgctca cagggaccgg agtcagaccg gatcgcgatc
    18061 gaacgcgcgc gcaagtgtgc ggtagtcgtc gagcgtgagc atctcggccc gggcttgcgg
    18121 cggcacgccg gcttccgcag tggcccgcgc aatctgctcg gaaggatagc cttcatcacg
    18181 cagggaattc accagcgtct tgcggcgatg ggcgaacgac gcgcgcacca gccgccggaa
    18241 ccgttcgtgc cggaccggct cgaacccttc ctgcgtgttg atccgaagat gcaccaccgc
    18301 cgatccgaca gtcggacggg gacggaaaca attcgcagat acacgaaagg ccagttccac
    18361 ctcggccgcc tcctgggtca acaccgacag caccccgtag tcctcgctgt tcggcttggc
    18421 cgccagtctg agcgccactt ctgtttgcag cataagtacc agacgatcga ggcgggcacg
    18481 cgcatcgagc agcgcgaaga gaatcggcgt agagacgtaa tagggaagat tcgccaccac
    18541 caccgtacgc agcggcaagg tctcgaaggg aaaggcgagg gcgtccccga tccgaaggtc
    18601 caggtttcgg caatgcccca gactttcctg cagatgaggc tgcaactgtg gatcgatctc
    18661 aacggcaatg acgcgtcccg cttcggc
//
LOCUS       DAOQRM010000037        18420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2133_length_18420_cov_12.391233, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000037 DAOQRM010000000
VERSION     DAOQRM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18420
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2133_length_18420_cov_12.391233"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PKK81_05020"
     CDS             complement(<1..317)
                     /locus_tag="PKK81_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32262.1"
                     /translation="MTDDYAQRKVQVIREFLAAGYPAPYTVEAVVPRRDPLADTKFKI
                     ILNNQVERTLAVCAAVVLDSLPTPDQLKQLLVSQGILEQVKASTDSRICHEALGTDEE
                     SHVR"
     gene            complement(716..1948)
                     /locus_tag="PKK81_05025"
     CDS             complement(716..1948)
                     /locus_tag="PKK81_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNV32263.1"
                     /translation="MSDFHQNGLVTVLHRLGASNLEQLEKELERHAASNPIALVLPSL
                     YSELENPALKHIVQVLKDIRYVNEIVISLDRASALEFRLAKQFFSVLPQRVRIVWNDG
                     AGIQDILKLLANTEIDTGLQGKGRGCWMAFGYVLARGQSNVIALHDCDILSYNREYLA
                     RLCYPIVNTNLGYEFCKGYYSRVTNRLHGRVTRLYLTPLIRSLQQVAGSHPLLTFLDS
                     FRYPLAGEFAMVRDLAWINRVPGDWGLEVGVLSEIYRNCALRRICQADIADAYEHKHQ
                     SLSADNAEQGLQKMCVDITKSLFRNLASEGVVLSEAVLKTLRATYLQTAQEAITRYQN
                     DAAINSLQFDRHEERMAVEVFLKGMKIATEAFLDDPLGVPMISNWSRVTGAIPDIFER
                     LIGAVERDHEWDPVGDRV"
     gene            complement(2079..3374)
                     /locus_tag="PKK81_05030"
     CDS             complement(2079..3374)
                     /locus_tag="PKK81_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32264.1"
                     /translation="METPELNDYRAHLLDALDDQPRRAGEERPEFLQVLVNHERVGTL
                     NCAHTDPSFSYTARERSIQHLEIRSESGVLVGGCVAPEAGQKSVRVPVRKGSVAIQVQ
                     NHFDGGSLRLQYAAAPGWWSHLATSIGLSSATTAPASSRTPVWTMTTALAQIVLVVGV
                     VALLAERIPTWLNAEDRAHQLEEELAARQSTQEQIDRVHQQLAQLVEAQASAVAVSRS
                     EQDRIAQLNALLDTVAHAQQKLNTQVVSVQEDLKTVKTDVAQEVETGLRVAVNAVEAD
                     RDLVRQDLRSIKSVNETLIKQVALLESRNRELHARLALTSLEVAKANAAPKPTVVAKG
                     DLPKEAPMATPVADNIREADRQAFMFWVAFQDGTTEKSIEDLVQELNGIKKGPMKSGW
                     YSVEVNLPNPEPPDRFLESVKRAKIVKSVVTSKAMPPAQ"
     gene            complement(3331..4293)
                     /locus_tag="PKK81_05035"
     CDS             complement(3331..4293)
                     /locus_tag="PKK81_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32265.1"
                     /translation="MSTKLPDGKTMRDGQIFSARLNNVVTTEREFDELVAKSLPELLD
                     RATGQTKRFLRETNQWGDDITHEKLALRWGYELVERFVVFGRTEVPCRPFFLLDSLIA
                     KSFSQPDPLCYHKELLTPVGRFLDGLASRAVVSRDALIALFYHFYGFSQAHVVKLLGF
                     GQAESQRVYKNFERWRQTGWQRTMNETGLSGSEIEVLEQELRTRPDHLNHEVDRLMPV
                     LQSHYRKSEPEHYPCLSEQKWGQLFHDDYGHDYRVWHLAFCRDCFLEVADRRQAEVSS
                     GLKPQVNLHIHPLKKGGVLALFGGDRGGRHHGNTRAQRLSRTSA"
     gene            complement(4487..6139)
                     /locus_tag="PKK81_05040"
     CDS             complement(4487..6139)
                     /locus_tag="PKK81_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNV32266.1"
                     /translation="MTIQTIGTAVLDRLHRLGVRHMFGIPGDYVLGLYKLMESSPIQH
                     VATTREDCAGFAADAYARINGIGALCVTYCVGGLNTVNAIACAYAERSPVVLLTGSPG
                     LSERARNPYLHHMVREFSTQREVFEKMTVAAVSLEDPVTAEREMDRAFAALLRYRRPI
                     YLEIPRDMVHVPLAQTSHKHQTQGEPTDRAALSEALNEVRTMLESAKRPVILAGAEVG
                     RFGLHDELTSLVERLNVPIASTLLGKSIIREDHPLYVGVYSGLVARDEVKEFVNQTDC
                     LLILGSILSDVEDLDAHSALFSDGHTIHATADRVAIKHHRYDSILFEDFVKGLAAASL
                     PSFPTPQLPAPYKPEEPMPPAQASVSLQGVFRHLDTVLHEKTLVIADVGESLFASVDL
                     HVHRRFEFLSPAYYTSMGFAVPAAIGAGFADPSLRPIVLVGDGAFQMTGSELSTAVRY
                     KQAPVVIVLNNHGYSTEREILEGPFNDIHEWRYERICELIGGGQGSRVATHGEFVQTL
                     GKALADPSQPHVINVLLDPADRSPAMMRLARRLAKRLSTDRP"
     gene            6362..7558
                     /locus_tag="PKK81_05045"
     CDS             6362..7558
                     /locus_tag="PKK81_05045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNV32267.1"
                     /translation="MTQLVTGSTAKVRLTAEREGPRYYGHLWVFDSNVADVLGTPAAG
                     DLVDVYTHQKRFFGRGLFNPHSKIRIRMLTFQEEPIDEEFFAARLRAAAALRRTVAPH
                     ATACRLVHGESDLLPGLVVDRFADMAVMQTLGYGMDLRKELLGELLVQEAGVKTVYLR
                     NDAKSRTLEGLPLSKGFLRGEGATTVNIHEGKAQFTVDIAEGQKTGWFCDQRENRIAA
                     ALFAKGKTVLEAFCHTGGFGIQAALAGAQSVEGLDVSAAAVALAQAHADQNQVAARCQ
                     YRQADAFEELRLLERNGQRYDLVILDPPAFARSKKAVPHAIAGYKEVNLRGLRLLQPG
                     GVLVTCSCSQPITDEDFWKMLQAAARDARRQIRLLEQRGQGPDHPVLAGMPETRYLKC
                     YLVQVF"
     gene            7563..9566
                     /locus_tag="PKK81_05050"
     CDS             7563..9566
                     /locus_tag="PKK81_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNV32268.1"
                     /translation="MMLEQDAPTEPVDAPLVPASVTLPEITLKAVVLSILLAAVLAGA
                     NAYLGLFAGMTVSASIPAAVVSMAVLRLFRQSNILENNIVQTAASSGEALAAGVIFTI
                     PGLVLIGYWTSFDYWQTFTVSLVGGVLGVLFTIPLRRALIIHARLRFPEGVATAEVLK
                     VGASSDAGAGGRVRLLLGAAALGGGFKFAEGGLKLWSESLEGAVQLGRSTLYGGLNLS
                     PALVAVGYIIGLNTAVVVFFGGAIGWLVLLPLYQAIIGSPEGLIGVAAAKATWSGQIR
                     YIGIGAMLVGGVWTLFQVRGPIWQSLHQLLALYGARNQSDGQSELLRTERDAGVVWLI
                     GLTVAALVPMVLLYQGLLNHNLWGGIGLTLLMVVTAFLFSAVAGYMAGLVGSSSNPVS
                     GVTIATIMLASLLLLGILGKGNPAGPAAALLVGAVVCCAAAMGGDNLQDLKTGHVVGA
                     TPWKQQVMQVIGVATGAVVIVPVLSLLQAKYGIGEVTAAHPHPLSAPQATLMANLANG
                     VFGGSLPWHLVGVGMVLGVVVIGLDLRQARRGAALRFPVLAVALGMYLPLKLSATILF
                     GGLLAEYVRAAGKFSDVPTEDRGLLCAAGLVTGEALVGILLALPIALSSIWPSLSGDP
                     FQLFADPPLGGWPGLAALIVVGLLLVCAARSAGQKDSATVHRH"
     gene            complement(9605..10075)
                     /locus_tag="PKK81_05055"
     CDS             complement(9605..10075)
                     /locus_tag="PKK81_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNV32269.1"
                     /translation="MPNVGTLIRAWRISQKCSEQALAERAGITASVLEALESEQADPP
                     ASTLEALAGALKIPLPWLFIHPTDLDLLCKDDDDEPAPLATLTGADPVLERILVAAGH
                     DRSLYVLLTALMQSSDPKLLRAAEVSLRSLVKQSKQATVPWQSRPPGHFEPPSD"
     gene            10251..10592
                     /locus_tag="PKK81_05060"
     CDS             10251..10592
                     /locus_tag="PKK81_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32270.1"
                     /translation="MLGTDIRGIMAEEEEVQRRQEALQSLMSMREKLLRESLEARIKR
                     ARGTGDWTNLSPAECANIYKEERVHLRAQLERLKAERDRTRGKLSALKRAKVRAQRIR
                     AAEAASGKKRK"
     gene            10661..>11424
                     /locus_tag="PKK81_05065"
     CDS             10661..>11424
                     /locus_tag="PKK81_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV32271.1"
                     /translation="MTSSPRTVSRNFLSHIRDVVKTKRTRDRERVFVLEGTKPILELL
                     QSAPQHIVCLVVTPTFLERQPPEVAQQIVSSGCTVHSCPEHQLAQLSDVETSSGVLAI
                     VHQPTWNQSAILAQPKIFGLYGDMLQDPTNMGTIIRTAAGLNVSALWLAPHSVDVFNP
                     KVVRATAGALFQLPIFPHTSLEELQNLDCVIMAADAGTSEGCVPIRAIRSIPARTVLA
                     IGSESRGLSEPVLDAATVRFTIPLKPGVESLNAAAAT"
     assembly_gap    11425..11524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11597..12958
                     /gene="radA"
                     /locus_tag="PKK81_05070"
     CDS             11597..12958
                     /gene="radA"
                     /locus_tag="PKK81_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNV32272.1"
                     /translation="MKAKTTFHCQACGHQALRWLGRCPDCGGWNTLKEERLPAAPKGR
                     QGMPKTAMAVATPISDIEIVGEPRHSTGMGEFDRVLGGGVVPGSVMLIGGDPGIGKTT
                     LLLQALPLLAAPGEQVLYVSGEESPRQIKMRGQRLGIDGKHLLILGETSLEQILKAIQ
                     EIQPAAVVVDSIQTVYTEQLTSAPGSISQVQEVAGQLMWFAKRSNVPVFIIGHVTKEG
                     AIAGPRLLEHIVDTVLYFEGDKSHSFRILRAVKNRFGSTNEIGVFEMKDGGLEEVSNP
                     SELFLAERPQRSTGSVVVSSLEGTRPILVELQALVSGTNYPMPKRMANGVEPNRLSLL
                     LAVMEKRLGMHLSGQDVYVNVVGGIHIDEPAIDLGIVAAVTSSLRESPIDFTTLVMGE
                     VGLGGEVRAISQAELRIREAAKMGFKRCLLPERNVAKLEPVEGIELIGIHEVGDALDA
                     VLA"
     assembly_gap    13016..13115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13116..13464
                     /locus_tag="PKK81_05075"
     CDS             <13116..13464
                     /locus_tag="PKK81_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32273.1"
                     /translation="RYRLDVVTAPGLNGTAPFVARRIWFDRRNLLVVQEERLSPSGDV
                     EATMQFDDYRSVGGVVEAVSAVNGQAPTADKRIMRPFAIRMTDGQGSGSLQVTFHELV
                     PNEPIKPSELGRV"
     gene            13461..14285
                     /gene="tsaB"
                     /locus_tag="PKK81_05080"
     CDS             13461..14285
                     /gene="tsaB"
                     /locus_tag="PKK81_05080"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNV32274.1"
                     /translation="MKAKPARHLLAIDTATAWQSVALLRDDQVLALIEQDAEGSHARS
                     LMGAIDRLLRGAGLSLKDLQALAVSIGPGSFTGLRVGLATMLGFRAVLGTPIVTVPTL
                     EAMAWNLRDVKGVLVPVLKSRHNEVYWAAYEWLPGTGLRTHIAEQVGPPASVARALQG
                     AKACTLFGDGWQAYEKDIREAVEAAGGQVREVRTEQQRPSAVSVGLAGRQRLGAGQVA
                     GPELVPCYVQRTEAEVKFDEQQGVSALERRRQRVASKLAQGRGKRGRTDADPQPRK"
     gene            14313..14813
                     /gene="rimI"
                     /locus_tag="PKK81_05085"
     CDS             14313..14813
                     /gene="rimI"
                     /locus_tag="PKK81_05085"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNV32275.1"
                     /translation="MASDSVLIEPATAEVLDEVFAIEQACFSAPWTRKMLAAELSGNQ
                     FAQFLIAKCPDPGSGAFVVAGYFCYWIVFEEVRLMNLAVLAPFRRQGVARRLVCTALR
                     AGLERGASRAMLEVRASNQEALTLYHRLGFRQTATRTRYYVNPDEDAVLMEMAPLQLG
                     PLCERS"
     gene            14882..15097
                     /locus_tag="PKK81_05090"
     CDS             14882..15097
                     /locus_tag="PKK81_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNV32276.1"
                     /translation="MQTETAITERLRRSNTEFRALEESHHRLDAELADLQKRHVLTPA
                     EEVLKKQLQKEKLATKDKIAEFIRSSR"
     gene            15203..15973
                     /gene="tatC"
                     /locus_tag="PKK81_05095"
     CDS             15203..15973
                     /gene="tatC"
                     /locus_tag="PKK81_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNV32277.1"
                     /translation="MLAPLAAHIQSLKKRLLIIVVTLAVAFATAFAYSSEMVAWLNRP
                     FPNQLVFYGPTEALFASIKVSFLAGIILSLPMVFYQCWKFIEPALLPKEQRWAIPLFL
                     LAAFLFALGLVFCNLVILPLVIDFFVSFGMDRDITPALGVGTYIDFNVKFLLIFGCAF
                     ELPLVLTLLSRVGVVSAAVLAHYRKHAIMAALIISAIVTPDATLFTMLLMAVPLMVLY
                     EIGIIGAKIFGRAAGPAPDMNLPLDPDIPIGTAGHRVR"
     gene            16009..17085
                     /locus_tag="PKK81_05100"
     CDS             16009..17085
                     /locus_tag="PKK81_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32278.1"
                     /translation="MLRHMLVLTAFTLGGLQGFAHAASFPGGSEGGAPLNLEQGHPGQ
                     EPGASADIGNTSIQALTAGSGGVIYAGSFGMGIFRSADRGGSWTSANQGLTDPFVLCL
                     TTGKDGAIYAGTFRGGVFRSRDGGKIWQPVNKGLKRLEIKALMMDAKGLYAGTGDGVY
                     LLNAAQDQWKVVSTGLDEILVHALARTDDGTLYAGTSGKGVHSYKERSTGWTRLRQGL
                     RDHEGLVENFIRVLAVDKEQGVYAGTFDGGVFRSGDAGKTWLPISRALPNDSIRGLIS
                     NERGVYVATGRGIFKTVDKGRQWVPLNKGLSNLSVQVLIEGEKGGFYAGTSSGVFRSD
                     DDGLTWTAVSQGLEGESVAPFKFN"
     gene            17103..17612
                     /locus_tag="PKK81_05105"
     CDS             17103..17612
                     /locus_tag="PKK81_05105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV32279.1"
                     /translation="MTAQAGSPVATISVTSKNEPWGEIVLRLFPDVAPGHVKNFVDLA
                     KKGFYNGTTFHRVIPGFMIQGGDPNSKNPDRASHGMGGPGHNVKAEFNSKPHKRGTLS
                     MARANDPDSAGSQFFICVNDANFLDWQYTVFGEVQSGLDVVDKVVGSKRDGRDNPLER
                     VEMTVTITE"
     gene            17712..18368
                     /locus_tag="PKK81_05110"
     CDS             17712..18368
                     /locus_tag="PKK81_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32280.1"
                     /translation="MTVFAAAALAAAGCAIPQVPSRTVYEDPVNFVRLELDANVLPEW
                     PPGHFTHPAQFSHDQVRRVLMGLTVQEHQASIQRWISGDSIRLPMFRDGEIAILVPQL
                     VEALRLARENERVTYYLSQPQTSVKRIITSGGLYVRGTELHFILGNWQTVYGIPAYGM
                     IYDRRYPMNPIVSKGFDLFFDLDQAMVIQSTSIWDWLLANSKDELVIDLARVFPGQPV
                     "
BASE COUNT         3375 a         5126 c         5892 g         3827 t
ORIGIN      
        1 cgcacatgtg attcttcgtc ggttcccaag gcctcatggc aaatccggga atccgtcgat
       61 gctttgacct gttccagaat gccctgcgag accaggagct gtttcagttg gtccggggtg
      121 ggaaggctgt ccagcacgac ggcggcgcag acggcgagag ttcgttccac ctggttgttg
      181 agaataatct tgaacttcgt gtcggctaac ggatctcttc gggggaccac ggcttccacg
      241 gtataggggg caggatatcc cgcagccaga aattctcgaa tgacttgcac cttcctctga
      301 gcgtaatcgt ctgtcatcgt tgctcgtcct tctgtttcct cgccaatgct tctttcgccg
      361 gcgttttgtg attcaacctg tgccggaatc ctacgccaat cgactggaag gcggcaagac
      421 gttcagaggc tatgatgtgt ggatgttcca ggagcggaat ctatcgagga ctgtggccgg
      481 attggcatga gaccgggacg atcgctctag gagggttggg gcagtcgtcg tctatcgcct
      541 atcgcagtca gccagcttgc ttcagcgcgc tctttgcaat tgctgcaaat ttcccgcatt
      601 gaggccaacg gaccctcttc atatcctttg agcgagcaca gcactctgca cttggtgcag
      661 atccaggatc catctgtcag tcgcatcgag atctccgtgg gtgttctgtc acccgttaca
      721 cccggtcccc gacgggatcc cactcatggt cgcgttccac cgcgccgatc aagcgttcga
      781 aaatatcggg aatggcgccg gtgacccggc tccaattgga gatcatcggc accccgagcg
      841 gatcgtccag aaacgcttcc gtggcgatct tcatgccctt cagaaagact tcgacggcca
      901 ttcgctcttc atgccggtcg aactgcaggc tgttgatggc cgcatcattc tgatagcggg
      961 tgatcgcttc ctgcgcggtc tgtagatacg tggcccgtag ggttttcaac acggcttccg
     1021 acagcaccac gccttcactc gcgaggtttc tgaagagaga cttggtgata tccacgcaca
     1081 ttttctgcag cccttgctcg gcgttgtcgg ccgacaggct ctggtgtttg tgttcgtagg
     1141 cgtcggcaat atcggcctgg cagatccgcc tgagcgcgca gttgcgatag atctcggaga
     1201 ggactccgac ttccagtccc cagtcgccgg gtacccggtt gatccaggca agatcccgca
     1261 ccatggcgaa ctcgccggcc aggggataac gaaaactatc caggaatgtc agcagcggat
     1321 gcgatccggc cacctgttgg aggcttcgga tcagcggcgt cagatacagc ctggtgacac
     1381 ggccgtggag gcgattcgtg acccggctgt aatagccttt gcagaactcg tagccgaggt
     1441 tggtgttgac gatcggataa cagagtcgcg cgagatattc ccggttgtag ctgagaatgt
     1501 cgcagtcgtg gagcgcgatc acgttgcttt ggccgcgggc caagacgtag ccgaatgcca
     1561 tccaacagcc ccggcccttg ccttgcagcc cggtgtcgat ttcagtgttc gccagcagct
     1621 tgaggatgtc ctgaatccct gccccgtcgt tccacacgat ccggacccgc tgcggcagca
     1681 ccgaaaagaa ttgtttagcc agccggaact ccagagctga ggcgcgatcc agagagatca
     1741 cgatttcatt gacgtaccgg atgtctttca gcacctgcac gatgtgcttc agcgcggggt
     1801 tctcaagttc cgaatacagc gaaggcagga cgagggcaat cgggttgctc gccgcgtgtc
     1861 gttccagttc cttttcgagt tgttccagat tggaggcgcc cagacggtgc aagaccgtca
     1921 cgagcccgtt ctgatgaaaa tccgacatgt tccctcctgt gccgggtgtg gagaggatga
     1981 tagaaagcaa atgaccagtg cggcagggag gcgccctcca cgcgggaggg ggacactcac
     2041 cgtgccgggt ggttgaattt gctgagggga aatcgcgact actgagccgg aggcatcgcc
     2101 ttgctggtca cgaccgactt cacgatcttg gctcgcttca cagactccag gaagcggtcc
     2161 ggaggttccg gattcggcaa gttcacttca acggaatacc agcccgactt catcggtccc
     2221 tttttgatgc cgttcagttc ctggaccaga tcctcaatgc tcttctcggt cgtgccgtct
     2281 tgaaacgcca cccagaacat aaaggcctga cggtctgcct cccggatgtt atccgcgacc
     2341 ggggtcgcca tgggcgcttc cttcggcagg tctcccttgg ccacgacggt cggtttgggc
     2401 gccgcgttgg ctttggcgac ttcgagcgag gtgagcgcca gccgggcatg taactcccga
     2461 ttgcggcttt ccagcagggc gacctgcttg atcagggttt cattgaccga cttgatcgac
     2521 ctcagatcct gccgcacgag gtcgcggtcg gcttccaccg cattgaccgc gacgcgcagg
     2581 ccggtttcga cttcctgggc gacatcggtc ttgactgtct tgaggtcttc ctggacggag
     2641 acgacttgtg tgttgagttt ctgttgtgca tgggcgacgg tatcgaggag ggcattcagc
     2701 tgggcgatcc ggtcctgctc cgaccgggac accgccacgg ctgacgcctg cgcctcaacc
     2761 agttgcgcca gttgctggtg cacacgatca atctgctcct gtgtggattg cctggccgcc
     2821 agctcttctt caagctgatg cgcccggtct tcagcgttga gccaggttgg aatccgttca
     2881 gccaacagcg ccacgactcc cacgaccagc acgatctgtg cgagggccgt cgtcatggtc
     2941 cagacgggag ttctgcttga agcgggtgcg gtggttgctg aggagagtcc gatcgacgtt
     3001 gccagatgag accaccatcc gggggcggcc gcgtactgga gtcgaagcga cccgccgtcg
     3061 aaatggtttt ggacctgaat ggcgacgctg ccttttctga cgggaacgcg aacacttttc
     3121 tggccggctt cgggcgcgac gcaccctccg accagcacac cggactcgct ccggatctca
     3181 aggtgttgaa tgctccgctc acgggccgtg taggagaagg acgggtcggt gtgggcgcaa
     3241 ttcagcgtcc cgacgcgctc gtggttgacc agtacctgga ggaattcagg acgttcctca
     3301 cccgcgcgcc gcggctgatc gtccagggcg tcaagcagat gtgcgcgata gtcgttgagc
     3361 tctggtgttt ccatggtgcc gtcccccccg atcccctcca aagagagcca agacgcctcc
     3421 ctttttcagc ggatgtatat ggagattgac ctgcggcttc aacccgctgc tgacctcggc
     3481 ttgccggcga tctgcgactt ccaggaagca gtcgcggcaa aaggccaaat gccagacgcg
     3541 gtagtcgtgg ccgtagtcat cgtgaaatag ttgaccccac ttctgttcag acaggcaggg
     3601 gtaatgctcc ggttcgctct ttcgataatg cgactgcaag accggcatca gccggtcgac
     3661 ttcatggttc aggtggtcgg gtctggtgcg taattcctgc tccagcacct cgatttcaga
     3721 cccacttaac ccggtttcgt tcatcgtgcg ttgccacccc gtttggcgcc accgctcgaa
     3781 gttcttatag acccgctggc tttcggcctg tccgaacccc aacaacttca ccacatgggc
     3841 ctgactgaac ccatagaagt gatagaacag ggcgatcagg gcgtcgcggc tgaccacagc
     3901 gcgagaggcc agcccatcga gaaaccgtcc taccggcgtc agcagctcct tgtgataaca
     3961 gagcgggtcc ggctgactga acgatttggc gatcaggcta tccaggagga agaagggacg
     4021 gcagggcact tcggtgcgac cgaagaccac aaaccgctcc accaactcat acccccagcg
     4081 cagcgccagc ttctcatgcg tgatgtcgtc gccccattga ttggtttcac ggagaaagcg
     4141 cttggtttga cccgtggcgc gatccaacaa ttccggcagc gacttggcga ccaactcgtc
     4201 gaactctcgt tccgtggtga cgacattgtt cagtcgtgca gagaagatct gaccgtctcg
     4261 catggtcttt ccatccggca gcttcgtcga catagtggcg aattccccgt caaatgacaa
     4321 ttcgacaagt acgcccctag aagaccacat ttgtccacgg agggtcaatg ggtgcggtgg
     4381 ccgcaacgcc gcactgtatc agcgtgaaac aggtatccgg aggtggggtg cgaggccatc
     4441 ccgtcggagg gcctgcgaag caacagaatc gtgatacgcg ctggggttag gggcggtcgg
     4501 tcgagagccg cttcgccagt cgccgcgcca gccgcatcat ggccggcgag cggtcggccg
     4561 gatcgagcaa cacattgatg acatggggct ggctgggatc tgccagtgcc ttgcccaggg
     4621 tctggacgaa ttccccgtgg gtggccaccc gggagccctg accgccgccg atcagctcgc
     4681 agatccgttc ataacgccat tcgtggatgt cgttgaaggg gccttccaga atttcgcgct
     4741 cggtcgaata gccgtggttg ttcaggacga tcacgaccgg tgcttgtttg tagcgcacgg
     4801 cagtcgagag ctccgacccg gtcatttgaa acgcgccgtc tcccaccagc acgatcggcc
     4861 gcaacgacgg gtcggcgaaa ccggcgccga tggcggccgg aaccgcgaac cccatcgacg
     4921 tgtagtaggc gggggagaga aattcaaatc gccggtgcac gtggagatcg acggaagcaa
     4981 agagtgattc gcccacgtcg gcaatgacga gggttttctc atggagcacg gtatcgaggt
     5041 gacgaaacac tccctgcaga ctgacggacg cctgcgccgg cggcatgggc tcttcgggct
     5101 tgtacggagc cggcaattgc ggggttggaa atgaagggag tgaggccgcg gccagtccct
     5161 tgacgaaatc ctcgaacaga atcgagtcat accggtgatg cttgatggcg acacgatctg
     5221 cggtggcatg gatggtatgg ccgtcagaaa agagggcgct gtgcgcatcc aggtcctcca
     5281 cgtcggacag gatggagccc aggatgagca ggcaatcggt ctggttgacg aattccttca
     5341 cttcatcgcg ggcgacgagt ccgctgtaga cgccgacata gagcggatga tcttctcgaa
     5401 tgatggactt gccgaggagc gtcgaagcaa tcggcacatt caggcgctcg accaggctcg
     5461 tcaattcgtc atggagcccg aagcggccca cttcggcgcc ggccagaatc accgggcgtt
     5521 tggccgactc caacatggta cgtacttcat tcaaagcttc gctcagcgct gcgcggtcgg
     5581 tcggttcgcc ttgggtctga tgtttgtgcg acgtctgggc caacggcacg tgcaccatgt
     5641 cgcgcggaat ttcgagatag atcgggcggc gataccgcag cagggccgcg aaggcgcgat
     5701 ccatctcacg ttcggcggtg acggggtctt ccaggcttac ggccgccacg gtcatttttt
     5761 cgaagacttc ccgctgcgtc gaaaactcgc gcaccatgtg gtgcaggtag gggttgcggg
     5821 cacgttccga caatccgggg gagccggtca gcaacacgac aggagatcgt tccgcatagg
     5881 cgcaggcgat ggcgttgacg gtattgagtc cgccgacaca gtaggtgaca cagagggcgc
     5941 cgatcccgtt gatgcgcgca taggcgtcgg ccgcaaaccc cgcacagtct tcccgcgtgg
     6001 tggccacgtg ttggatggga gaagactcca tcaatttgta aaggccgagg acgtagtcgc
     6061 cggggatgcc gaacatgtga cggactccga gccggtggag ccgatccagg actgctgttc
     6121 cgatcgtctg tatggtcatg gtgcctcggc tgtcgagtga gaggtcgtga atttgcgtac
     6181 tgtccgcccg tcttcctgca gcaaaccata ggtggtaggg aaggtcaagc acaggagcga
     6241 tcgtcacatc ggcatgctgc ccgcccactg tccggttgct tcgcgggatt atttcaggca
     6301 ccctcgtgag ccatttcacc atcgtcgaat caaacagctt atacgggacg taaccattac
     6361 catgacacag cttgtgaccg gttctactgc gaaagtccga ctgacagccg aacgtgaagg
     6421 gccgcgttac tacgggcacc tgtgggtgtt cgacagcaat gtggccgacg tgctgggcac
     6481 accggctgcc ggggacctcg tcgatgtgta cacccatcag aaacggtttt tcggccgcgg
     6541 cctcttcaat ccccattcca agattcgcat ccgtatgctc acgtttcagg aggagccgat
     6601 cgacgaggag ttttttgccg cgcgtctccg tgccgcggcg gcccttcgcc ggacggtcgc
     6661 gccccatgcc acggcctgcc gcctcgtaca tggtgaaagc gatctcctgc cgggcctggt
     6721 cgtggaccgg tttgctgata tggcggtgat gcagacgctg gggtacggca tggatctgcg
     6781 aaaggaactc ttaggcgagt tgctggtaca ggaagcgggg gtcaagacgg tgtacctccg
     6841 caatgatgcc aagagccgca cactggaagg gctgcctttg tcaaaaggat ttctgcgcgg
     6901 tgagggtgcc acgacggtaa acattcacga ggggaaggcc cagtttacgg tcgatattgc
     6961 ggaggggcag aagaccggct ggttctgcga tcaacgggaa aaccggatcg ccgcggccct
     7021 cttcgcaaaa gggaaaaccg tgttggaagc cttttgccac accggcgggt tcggcattca
     7081 ggccgcgctg gcgggggcgc agtcggtgga agggttggat gtgagcgcgg cggccgtggc
     7141 gctggcccag gcgcatgcgg atcagaatca ggtggccgcc cgttgtcagt atcgccaggc
     7201 cgatgccttt gaagagttgc gcctgttgga gcgtaacggc caacggtacg acctggtcat
     7261 tctcgatcca ccggcgttcg cgcgtagcaa gaaggcggtg ccgcatgcca tcgccgggta
     7321 caaggaagtg aatttgcgcg gccttcgtct gcttcagccg gggggcgtgc tggtgacctg
     7381 ttcctgctcg cagccgatca cggacgagga cttctggaag atgctgcagg cggccgctcg
     7441 ggatgcgcgc cggcagatca ggctccttga acaacgtgga cagggtccgg accatccggt
     7501 gcttgcgggc atgccggaaa cgcgctatct caaatgctac ctcgtgcagg tgttctgagg
     7561 ggatgatgct tgagcaggat gcgccaacgg agccggtaga cgcgccgctc gttcccgcct
     7621 cggtcacgct gccggaaatc acactgaaag cggtcgtcct gtcgattctg ctggccgccg
     7681 tgctggccgg agccaacgcc tatttgggcc tgtttgccgg catgacggtg tccgcctcca
     7741 ttccagccgc cgtcgtctcc atggcggtgc tgcgtctctt ccgtcagtcg aatattctcg
     7801 aaaacaatat cgtgcaaacc gccgcctcct caggcgaagc gttggcggcc ggggtgatct
     7861 ttaccattcc ggggctggtc ctgatcggct actggacctc ttttgactac tggcaaacgt
     7921 tcacggtgtc gctcgtcggc ggggtactcg gggtgttgtt caccattcca ttgcgccgtg
     7981 ccctgattat ccacgccagg ctgcgatttc ccgaaggggt tgcgacggcg gaagtcctga
     8041 aggtcggagc ctcgtcggat gccggtgccg gtggccgggt tcgcctattg ttgggcgctg
     8101 cggcgctggg tggcgggttc aagtttgccg agggaggatt gaagctgtgg agcgagtcgc
     8161 tggaaggggc cgttcaactt gggcgttcga cgttgtacgg agggctgaac ctgtcaccgg
     8221 ccttggtggc ggtcgggtac atcatcgggc tcaacacggc cgtcgtcgtc tttttcggcg
     8281 gagcgatcgg ctggctggtc ctgctgccgt tgtaccaggc gatcatcggt tcgccggagg
     8341 gattgatcgg cgtggcggcg gcgaaagcga cgtggagcgg gcagattcgc tacatcggca
     8401 tcggggctat gctggtgggt ggggtctgga ccctgtttca ggtgcgtggc ccgatttggc
     8461 agagtctgca tcaactcctg gcgctgtacg gcgcccgcaa ccaatctgat gggcaatcgg
     8521 aacttctgcg cactgagcgg gatgcgggag tcgtctggct gatcgggctg acggtggcgg
     8581 cactggttcc catggtgctg ttgtatcaag ggctgttgaa tcacaatctg tggggcggca
     8641 tcggattgac cctgctcatg gttgtgacgg cgtttctctt ttctgccgtg gctgggtaca
     8701 tggctggtct ggtcggcagc tcgagcaatc cggtttcagg tgtgacgatt gccaccatca
     8761 tgctcgcatc actgctcctg ctggggattc ttgggaaggg caatccggcc ggtccggctg
     8821 cggctttgct ggtaggggcg gtcgtctgtt gcgccgcggc gatgggcggc gacaatttgc
     8881 aggatctgaa aaccgggcac gtcgtcggtg cgaccccgtg gaagcaacag gtgatgcagg
     8941 tcatcggcgt ggcgaccggc gcggttgtca ttgtgccggt gctgtccctg ctgcaggcga
     9001 aatatggaat cggtgaagta acggccgccc atccccatcc gctctcggca ccacaggcca
     9061 ctctcatggc caatcttgcc aatggagtgt tcggcggttc cttaccctgg catctggtgg
     9121 gcgtggggat ggtgctggga gtcgtcgtga tcggtctcga cttgcggcag gcacgacggg
     9181 gtgccgccct tcgtttccct gtcctggcgg tggcgcttgg tatgtacctg ccgctcaagt
     9241 tgtcggccac gatcttgttc ggtggtttgt tggccgagta tgtacgggcc gccgggaaat
     9301 tttccgatgt tccgacggag gatcgaggtc tgctgtgcgc cgctggttta gtcacaggtg
     9361 aagcgctggt cgggatcctg ttggcgcttc ccattgccct cagttcgatc tggccgtcac
     9421 tctcgggaga tccgtttcaa ttgtttgccg atccgccgct tggtggatgg ccggggctcg
     9481 ctgcactgat tgtggtgggg ttgctgctgg tgtgtgccgc ccggtcggcc ggacaaaaag
     9541 attcagcgac tgttcatcga cactgatggt gtgggacggt gtagaggtgg tcggcgaggg
     9601 ggcgctaatc gctgggtggt tcgaaatgtc ccggtggccg tgattgccag gggaccgtcg
     9661 cctgcttgga ttgtttcacg aggcttcgca ggctcacctc tgcggcgcgt aggagtttag
     9721 gatcgctgct ttgcatgagc gccgtcagga ggacgtatag cgaccggtca tgtccggccg
     9781 ccacgagaat tctctccagg acgggatcgg ctccggtcaa tgtggcgagg ggggccggct
     9841 catcgtcatc gtctttacac agcaggtcta aatccgtggg gtggataaag agccagggga
     9901 ggggaatctt gagtgcgccg gccaacgctt ccagggtgga ggccggagga tcggcctgtt
     9961 ccgattccag cgcttccagc accgaggctg tgatcccggc tcgttctgcg agcgcctgct
    10021 cggagcactt ttgcgagatc cgccaggcgc gaatgagagt gccaacgttc ggcatggacg
    10081 gatcttactg tatccctttc cgggtctgca acggcgcatt tccgaggctc atccgatact
    10141 tgcctgtaac atcaacgtgt tacgatccat tctcctaggt agaacaaagc cgctcaaatc
    10201 gggtagaatg aggcgttcta attgttgtct cgagcgagga ggatcagccg atgttaggaa
    10261 ccgacattcg cggcattatg gccgaagagg aagaagtcca gcggcggcag gaagccttgc
    10321 agtcattgat gtcgatgcgc gagaagctgc tgcgtgaatc gttggaagcg cgaatcaagc
    10381 gggcgcgtgg aacgggagac tggacgaacc tgtcgcctgc ggaatgtgcc aatatttaca
    10441 aggaagagcg cgttcatctt cgtgctcagc tggagcgatt gaaggccgag cgggatcgga
    10501 cccgcggcaa gctgtcggcg ctcaagcgag cgaaggttcg tgcgcaacgc attcgcgcgg
    10561 cggaagcggc ttccggtaag aaacgcaaat aggtcgatgt cggggaggcc ttgcctgttc
    10621 agtaaggcct cctcgcagtg tcgcagactt tgggtaactg ttgacctcct cacctcgcac
    10681 cgtttctcgc aactttctct cccacatacg ggatgtcgtc aagacgaagc gtacgcgtga
    10741 ccgcgaacgg gtcttcgtgc tcgaaggcac caaacccatc ctcgaattgt tgcagtcggc
    10801 acctcaacac atcgtctgcc tcgtcgtcac ccccacgttt ctcgaacgac aaccgccgga
    10861 agtcgcgcag cagattgtca gcagcgggtg tactgtccac agctgtccgg aacaccagtt
    10921 ggcccaattg tccgatgtcg aaacctccag cggcgtgctg gccatcgttc atcagccgac
    10981 ctggaaccag tcggccatac tggcgcaacc gaaaattttc ggcctctacg gggacatgct
    11041 gcaggacccg acgaatatgg ggacgatcat tcgaactgcg gcgggactca atgtgagtgc
    11101 cttgtggctg gcaccccact ccgtcgatgt gttcaatcca aaagtcgtgc gggcgaccgc
    11161 aggcgcgttg tttcagctgc ccatctttcc ccacaccagc ctcgaagaac tgcagaatct
    11221 ggattgcgtg atcatggccg ccgacgccgg aaccagtgag ggctgcgtgc ccatccgggc
    11281 gattcggagt attcctgcgc gaacggtgct ggcgatcggc agcgagagtc ggggcctgtc
    11341 tgaaccggtt ctggacgccg ccaccgtgcg atttaccatc ccgttgaaac cgggggtcga
    11401 atctctcaat gccgcggcgg cgacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnctcctt cctttttctc tgctgtttca tttacgatgc cttgagatct gtgaccttga
    11581 ggtggagcga atagagatga aggcgaagac gacatttcat tgccaggcct gcggccatca
    11641 ggcgctgcga tggcttgggc gctgccccga ttgtggaggg tggaacacgc tcaaggaaga
    11701 gcgattgccg gctgccccca aaggccgcca gggtatgccc aaaacagcca tggccgtggc
    11761 cacgccgatc tccgatatcg aaatcgtcgg cgaacccagg cacagcacgg gcatggggga
    11821 gttcgaccgt gtgttgggcg gtggtgtggt gccaggctcg gtgatgctga tcgggggcga
    11881 tcccggcatc ggcaagacca cgttactctt gcaggcattg ccgctgcttg cggcgccggg
    11941 tgagcaggtt ctgtacgtct ccggcgagga gtctccccgg cagatcaaga tgcgggggca
    12001 acgtctgggc atcgacggca aacatctgct gatcctcggg gaaaccagtc tcgaacagat
    12061 cttaaaggcc atccaggaaa tccagccggc ggccgtggtg gtggattcga ttcagacggt
    12121 ctacaccgag cagctcacct cggcgccggg cagcatcagc caggtgcagg aagtggccgg
    12181 gcagctgatg tggttcgcca agcgcagcaa cgtgccggtg ttcatcatcg ggcatgtcac
    12241 gaaggaaggc gccatcgccg gaccgcgctt gctggaacac atcgtcgata cggtactgta
    12301 tttcgaaggg gataagagcc acagtttccg gattctgcga gcggtaaaaa atcgcttcgg
    12361 atcgacgaac gagatcggcg tctttgaaat gaaggatggc ggcttggagg aagtcagtaa
    12421 cccctccgaa ttgtttctcg ccgagcggcc gcaacgcagc accggctcgg tggtggtgtc
    12481 cagcctggaa gggacgcggc cgatcctggt ggaactgcag gcgttggtct cggggaccaa
    12541 ttatcccatg cccaagcgta tggccaatgg cgtggaaccg aatcggctgt cgttgctgct
    12601 ggccgtgatg gagaaacgat tgggcatgca cctctcaggg caggacgtgt atgtgaatgt
    12661 ggtcggcggg atccacatcg acgaaccggc cattgatctg ggcattgtcg ccgccgtcac
    12721 gtcgagtttg cgggagagtc cgatcgattt tacgacgctc gtcatgggcg aagtcggact
    12781 gggtggtgaa gtccgggcga tcagccaggc cgagctgcgg attcgcgaag ccgccaaaat
    12841 gggattcaaa cgctgtctcc tgccggagag aaacgtcgcc aagctggaac cggtcgaagg
    12901 cattgagttg atcggcattc atgaagtcgg agacgcgttg gatgcagtac tggcgtgaag
    12961 caaaaggtga aacggtagag gtgacacgca agactgcctc gctcggtacg gttgtnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccggt atcgactcga tgtggtgacc
    13141 gcgccgggcc tgaatggcac ggctccgttc gtggccaggc ggatctggtt cgatcgtcgg
    13201 aatttgctgg tcgtgcagga agagcgcctg tcgccgtccg gtgacgtcga agccacgatg
    13261 cagttcgacg actaccgctc ggtgggcggt gtggtcgagg cggtatcggc ggtgaacggc
    13321 caggcgccta cagccgataa gcggatcatg cgccccttcg ccatccggat gaccgacgga
    13381 cagggctcgg gcagtttgca ggtgacgttt cacgaattgg tgccgaacga accgatcaag
    13441 cccagcgagc ttgggcgggt atgaaggcga aaccggcccg gcatttgctg gcgatcgata
    13501 cggccacggc ctggcaaagt gtcgcgctgc ttcgcgatga tcaggtgctg gccctgatcg
    13561 aacaggatgc cgaaggatcg cacgcgcgct cgttgatggg cgccatcgat cgtctgttgc
    13621 gcggggccgg actttcgttg aaggatcttc aagcattggc ggtgtccatc ggccccggtt
    13681 cgttcaccgg cctgcgtgtg gggcttgcca ccatgttggg atttcgtgcc gtgttgggca
    13741 ctcccattgt gacggtgccg accttggaag cgatggcctg gaatctgcgc gatgtgaagg
    13801 gtgtgttggt gccggtcctg aagagtcggc acaatgaagt gtattgggcc gcctacgaat
    13861 ggttgcctgg aacgggtttg cgaactcaca tcgccgagca ggtcggtcct cctgcttcgg
    13921 tggcccgggc gttacaaggg gccaaagcct gtacgttgtt cggcgacggc tggcaagcct
    13981 atgaaaagga catccgggag gccgttgaag cggcgggcgg gcaggtgcga gaagtcagga
    14041 cggagcaaca acgcccttca gccgtgagtg tcggtctcgc cgggcgacag cgcctggggg
    14101 cagggcaggt tgcgggaccg gagctggttc cctgctatgt tcagcgcacg gaagcggaag
    14161 tgaaattcga cgagcagcag ggggtgtcgg ctctcgaacg gcgccggcag cgggtcgcca
    14221 gcaaattagc gcagggccgt gggaaacgcg gtcgaaccga cgccgatccg cagccgcgga
    14281 agtaggcaat ggcattggga attgagtcgg atatggcgtc agattcggtg ctgattgaac
    14341 cggcgaccgc tgaggttctc gacgaggtat tcgccatcga acaggcttgc ttctctgccc
    14401 cctggacgcg caaaatgttg gcagccgagt tgtccggtaa tcaatttgcc cagttcctta
    14461 tcgcgaagtg tcctgacccc gggtcagggg ccttcgtggt ggccgggtat ttttgctatt
    14521 ggatcgtgtt cgaggaagtg cggttgatga acctggctgt gctcgccccg tttagacggc
    14581 agggtgtggc gaggcggctg gtctgtacgg cattgcgggc cgggttggag cgtggtgcca
    14641 gccgggcgat gctggaggtg cgggcctcga atcaggaggc gttgaccctc taccatcggt
    14701 tgggatttcg tcagaccgcc acgcggacgc ggtattatgt gaaccctgac gaagatgcgg
    14761 tgctgatgga aatggcgccg ctccagttag gtcccttgtg cgaacggtcg tagggcgatt
    14821 ccgggagccg ggcaacagga cagtcatcca cgaacgtatg tagtcaacca ggaggtgcga
    14881 catgcagacg gagacggcga tcactgaacg gttacggcgg tccaatacag aattccgtgc
    14941 gcttgaagaa tctcaccacc gcctcgatgc cgagttagcc gatttacaaa agcggcatgt
    15001 gctcacgccg gcggaagaag tgctgaagaa gcagttgcaa aaagagaagc tggccaccaa
    15061 agacaaaata gccgaattca ttcgatcgtc gcgttagcga agatctccgg ctttccgtcc
    15121 cggtcgcatc ccgccgctgc tcgcatcgag ccggtgtgag agccgaagga gcggaaggcc
    15181 gggtcgttga ttcagacagg caatgctcgc accactggcc gcccatattc agtcgctcaa
    15241 gaagcggctg ctgatcatcg tggtgacctt ggcggtcgcg ttcgccacgg cgtttgccta
    15301 ctcctccgag atggtcgcct ggctcaatcg cccgtttccc aatcaactgg tgttttacgg
    15361 gccgacggaa gcgctgtttg cgtccatcaa agtgtcgttt ctggcgggga tcatcctcag
    15421 cctgcccatg gtgttttacc agtgctggaa gtttatcgaa ccggcgttgc tccccaagga
    15481 gcaacgctgg gcgattccct tgttcctgct ggcggcgttt ctgtttgcgc tcggcctggt
    15541 cttttgtaac ctcgtcattc tgccgctcgt catcgacttc ttcgtcagct tcggtatgga
    15601 tcgcgacatc acccccgcgc tcggtgtagg gacctatatc gacttcaacg tcaaatttct
    15661 cctgatcttc ggctgcgcct ttgaactgcc gctggtgctg actctgttgt ctcgcgtggg
    15721 ggtcgtctcg gccgcggtgc tggcgcacta tcgcaaacat gccatcatgg cggcgctgat
    15781 catttcggcc atcgtgaccc cggacgcgac gctgtttacc atgttgctga tggccgtccc
    15841 gctcatggta ctctatgaga tcgggatcat cggggccaag atcttcgggc gggccgcagg
    15901 gccggctccc gacatgaatt tgccgctcga tcctgatata cccatcggca cggccggtca
    15961 tcgcgtccgg taatccggcg cccacacttt tggaaggatg atcattctat gctgcgtcac
    16021 atgctggtgt tgacggcgtt tacactcggc ggtctgcagg ggttcgcgca tgcggcatct
    16081 ttcccgggcg ggagcgaggg cggtgctccg ttgaatctgg agcaggggca tccggggcag
    16141 gaaccgggcg cctccgctga tatcggcaat acgagtattc aggccctcac ggccggctcc
    16201 ggaggagtga tttatgccgg ttccttcggc atgggcattt ttcgcagcgc cgatcgtggc
    16261 gggtcctgga cgtcggccaa ccagggattg accgatccct tcgtgctctg tctcaccacc
    16321 ggcaaggacg gcgcgatcta cgcgggtacg tttcgcggcg gcgtcttccg gtcgcgtgac
    16381 gggggcaaga tctggcagcc ggtcaacaag ggcctcaagc gtctggagat caaagccttg
    16441 atgatggatg ccaaggggct ctatgccgga accggggacg gcgtctatct gttgaatgcc
    16501 gcgcaggatc aatggaaggt cgtgtccacc ggtttggatg agattctggt gcacgcgctg
    16561 gccaggaccg acgatgggac cctctacgca ggcacctccg ggaagggcgt gcatagttat
    16621 aaggagcggt cgaccggatg gacgcggttg cggcaagggc tgcgcgacca cgagggattg
    16681 gtcgaaaatt ttatccgggt gctggccgtg gataaggaac agggtgtcta tgccggcacg
    16741 ttcgacggcg gtgtcttccg cagcggcgat gcgggcaaaa cctggttgcc gatcagccgg
    16801 gccttgccga acgattcgat ccgcggtttg atcagcaatg agcgcggcgt ctatgtcgcc
    16861 acgggccggg gaatcttcaa gaccgtcgac aaggggcggc aatgggttcc cttgaacaag
    16921 gggctcagca atctgtcggt gcaagtgttg atcgaaggtg aaaaaggggg cttctacgcc
    16981 ggcaccagtt cgggggtctt tcgcagcgat gacgatggcc tcacctggac ggcggtcagc
    17041 cagggattgg aaggggagag tgttgcgccc ttcaagttta actgagtagg aaaggatacg
    17101 agatgacggc acaagcgggt tcaccggtag cgaccatttc tgtcacatcc aagaacgagc
    17161 catggggcga gatcgtgctg cgtttgtttc ccgacgtggc gcccggtcat gtgaagaact
    17221 tcgtagatct ggcgaagaag ggcttctaca acggcacgac gtttcaccgg gtgatccccg
    17281 ggttcatgat tcagggcggc gatcccaata gcaagaatcc ggaccgcgcg tcgcatggaa
    17341 tgggtggtcc agggcacaac gtgaaggcgg agttcaacag caagccgcac aagcggggga
    17401 ccttgtccat ggcgcgcgcc aacgatccgg acagcgcggg ctcgcagttt tttatctgcg
    17461 tcaacgacgc caactttctg gactggcagt atacggtctt cggtgaagtg caaagcggac
    17521 tggacgtggt cgacaaagtg gtcggctcca agcgcgacgg gcgggataac ccgctggagc
    17581 gcgtcgaaat gaccgtcact attaccgagt gatggccgtg aaacgtaaaa aataacacgt
    17641 acgacgtgaa gaggggaaac tcaaagagag aggggcgggt cggccgacgt ttcgcgtttc
    17701 acgtttcacg gatgacggtg tttgccgcag cggccttggc cgctgcgggg tgtgccatcc
    17761 cccaggtccc ctcgcggacc gtctacgaag atcccgtcaa ttttgtccgc ttggagttgg
    17821 atgccaatgt gctgccggag tggccgcctg gtcatttcac gcatccggcc cagttctctc
    17881 acgaccaggt gcgtcgcgtt ctgatgggcc ttacggtcca ggagcaccag gcttcgatcc
    17941 agcgctggat ttcaggggac tcgatccggt tgccgatgtt tcgggatggg gagatcgcga
    18001 ttctggtccc gcagttggtc gaagcgttac ggttggcgcg ggagaatgag cgggtcacct
    18061 attacctgag tcagccgcag acgtcggtca aacggatcat cacgtcaggc gggctctacg
    18121 tgagggggac tgaactgcac tttattttgg gaaattggca aaccgtgtac ggtattccgg
    18181 cctacggcat gatttacgat cgccgctacc cgatgaatcc catcgtctcc aaaggcttcg
    18241 acctgttttt cgatctcgac caggccatgg tgattcagag taccagcatc tgggactggc
    18301 tcctcgccaa ctccaaagac gaactggtga tcgaccttgc cagagtcttc cccggccaac
    18361 cggtctagcc cggactattc ctgaccgccc gctccgtcgt ccgatcctct cgcccggcgg
//
LOCUS       DAOQRM010000038        18332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2149_length_18332_cov_17.512830, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000038 DAOQRM010000000
VERSION     DAOQRM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18332
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2149_length_18332_cov_17.512830"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1298
                     /locus_tag="PKK81_05115"
     CDS             219..1298
                     /locus_tag="PKK81_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV32281.1"
                     /translation="MTLRRTTYLVIFTLLALSAMLAVKWTVLTPVPASEVTGTVPAGT
                     AVVLAPVTTEAIRHTIKAVGSLRANESIMIRPEIAGRIRQVWFQEGQAVEKDQLLIEM
                     DDSELQAELAQAAAQLKVSRLTYERLKRLDLDGKRYVTKQQLDEVAGALQVSQANHAL
                     YVTRLAKTKIRVPFTGVTGIRRVSPGDFVGAGRDLVNLEDLDTLKIDFKVPETMLRHL
                     APGQSLELTTDAYPGNTFHGTVYVIDPQVDPSTRSVQVRARIPNPQLRLRPGMFAQVL
                     LTFGQEERALLIPEEAVMPKQDKAYVYLAQDGTAQQREVTLGTRTRGFVQVLDGLTEQ
                     DTVIRVGHHKLQNGDSILALSSTPH"
     gene            1321..4437
                     /locus_tag="PKK81_05120"
     CDS             1321..4437
                     /locus_tag="PKK81_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV32282.1"
                     /translation="MRLSALSIERPVLAAVATLLLMLFGLLSYSRLPVREYPDTTFPI
                     VSVMTVYPSADAQLVETDITSVLEDALSGVEGLRTLRSTSREGLSTIGLEFSLMRNLD
                     AAANDVRDRVTRVQYLLPQGIEAPLVMKEDSDSDGIMWLALISDRHTELEMTDFAERQ
                     LKDRLAPLPGVSSVMLDGGRRYAMRIWLDPDRLASRLLTVQDVEDALRTQNVSMPTGR
                     IESTQREFSVRMGGGLEKAAQFNQLILAYRDGYPVRLQDVGAAEIGPEDDRKLVRVNG
                     KPAIGLGIMKQSKANTLEAARAVKRTIPAMEALLPEGMQLVTAFDSSIYIERSLHEVY
                     EAVGLSVLLVVLVVFLFLRSARATLIPAVAIPGSILGTFVIMQMTGSSINTITLLGFV
                     LAIGLVVDDAIVVVENVYRRIERGLSPTQAAIEGSREIGFAVISTTLTLAAVFFPIIF
                     LPGIVGRLFAELGIAVAGSVLLSGFIALTLTPAMCARLLGKTAGTASPSRGSALDPSA
                     WLRWLTQRYRLALKVALERPAIILGGAVLSLIVSAALMWGLPSELAPLEDTGWFAVHV
                     SAPEGSTLDYTDRYARETENATARIPEMDSYYTVVARGWRPTLVNRAVTWVTLKDWSD
                     RNRVQQEIVDDVQPALSAIPGATVFALNPPPFNQEDSKTPVQLVIRGPSYEVLDKIVT
                     QIRHDLTGNQEVVNVESDLDLNKPELRVEINRDKAADLGIPIETIGRTLEVFLGGRKA
                     SMFMREGKEYPVIMQTRPDQRGTRSDIEHLQVRGTQGDLIPLSNLVTLRETVAPKQLN
                     HYEKLRAVTISAGVGADSTLSRSIESLEQTIRKHLPPGATISYAGETKEFKESSGNLH
                     LTFLLALLVVYLILAAQFESFRHPITVLVSVPPALAGALLALLLFRGTLNIYSEIGLM
                     ILIGLVTKNAILIVEFANQLCQEGKSPRAAIVEAAVLRLRPIVMTTLSTILAALPLAL
                     ATGAGAAGRWHIGLVVINGLVLSTLLTLFLVPVVYLLFARTAPVRQSAAVPEPFSEVL
                     PRHSPSQG"
     gene            complement(4582..11760)
                     /locus_tag="PKK81_05125"
     CDS             complement(4582..11760)
                     /locus_tag="PKK81_05125"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNV32283.1"
                     /translation="MHQDIEIEGLRLSPHQKRLWLWQSRGHVGQARALFRLDGVFDDE
                     AVQKALRHAIARHESLRTTFYRESGMKVPFQVINEESDVLWRRVDLRHLPDEGEREQA
                     AGEAARRTDDRDWEATPLVRATLCRLTDDHARLVLDLPGLCADTWSLSNLAAELSQLL
                     DGVSADWKDEQPVQYGQFSEWHYELLDGEEAKQGQAWWARRRPTGDHAVPLPFEQAGR
                     PEGRQAAARPVHRICSSTETERLRGAAAAAQVSLAVLLYACWSALLYRLTGRRDLVCW
                     WRADGRPYEELQGGIGLFERWLPLHLRIEGNMALRDLLSLAAQEQGQAEDWQHYYPAE
                     QDDEDAAAVREGIGFEYGRRAKPSRTAAGVITLAESTFWAEPFKFRLACLEDEADLAL
                     TWHIDGERIPSQMVQSLADRYVELLRAIAGRLATAIDDLDIVGTRERTRLTRELNQTA
                     RPLGPSFPMHRLIEAQAARRPSSPALVAGERRLSYDDLNRQANQVAHGLRRRGVQPGD
                     RVGLCLDRSADMIVAMLGVLKAGAAYVPIDPAHAAVRLAPQMAQSGARLLVTQSVSEQ
                     ARPLFDGVILDVGEAVANEPDGNLNLALSPDDLAYVIYTSGSTGQPKGVAVSHRNLAN
                     YTLAILRQLDLREPLQFATVSTLSADLGHTVIFPALTSGGCLHVINYETATDGRLFAS
                     YLSEHPIDVLKIVPSHFKALVATADGQALWPRKFLLFGGEALSWDLVEQVRRQAACIV
                     INHYGPTETTVGALTTVVGDNEDVRLARTVPIGRPIDNLEAYILDERQEPVPVGAAGE
                     LYLGGAGVARGYWEQPDRTAERFLPNPHASQPGARLYRTGDLARRLPDGSIEFLGRVD
                     HQVKIRGFRIELGEIESVLRQHPAVQDAVVTAREDGNAELYLAAYLVARGAEPDRQAI
                     QDFLRSHLPDYMVPTAVSSLPALPLTANGKVDRRALPEPDREGSETIRFVAPGTATEE
                     ILAAIWSEVLKREGIGIHDNFFDLGGHSLLATQVMSRLRQAFRVDLPLRTVFEAPNIA
                     QLARVIDAAKGIAGGDQPPPMQPWPRACSLPLSFAQERLWVLAQLDPEGVAYNIPIAL
                     RVTGPLDVAALERSFNEVVRRHESLRTTFQSLNGVPVQVVASSLTLTVTVIDLQHLPV
                     EARDQAAVRLAGVESQRPFDLRYGPLLRVSLIRLQPNEQVLLLTLHHIVSDAWSAHVL
                     VKEVTALYDAFAAGRASSLSDLPLQYGDFSQWQRAWLSGTVLKRELEFWRTSLGGDLP
                     VMELPTDRPRPALQTSRGAMASVTIPAELSVKVSALSRKMGATLYMTLLSAFFILLAR
                     MTGQTDLIVGTPIANRTRKETEGLIGFFVNTLAIRTKVFGHSSMADVLANVREACLDA
                     YAHQDMPFEKLVDALQPKRDVSRSPVFQVMFDLQNAPVSELNIQGLEFQPIEIDTTTA
                     KFDLSMTVQDQDGRLLVAMEYNSDLFVASTIERLLDRYQQVLAGMTGDMRIGVGSIHL
                     LSAQERQAVLTVNRTHASSGPSQSLPALIERRAAQTPDAPALVLQDHVVSYRDLNDKS
                     NRLARWLRSRGVESERRVGICLERSFDMVVALLGIMKAGGAYVPIDPTYPAGRQHTLA
                     VDAGVDLVLTQGTATPDPDTFPVPVLSLDRLNAELETLSGENLDRPISADQAAYVIYT
                     SGSTGKPKGVVVSHRALCQHLVAAAAAYKFGPSDRVLQFAAVSFDVASEELWTTLISG
                     AAVVLQPCRLFDSIPAFLDFLDRRHVTVAGLPASYWHQWVDAFEQADCSVPSRVRLLI
                     VGNEPVASSDLNRWRRQVGSNVRWLNAYGPTETIITASLYEPPLSVEEFALHTVPIGR
                     PLPGRSMYVLDRHLEPVPFDVPGEVYIGGETLARGYLDDPERTAGLFIPDPFGGRPGA
                     RLYRTGDRALVRSDGTIEFHGRFDDQVKLRGFRIEPGEIEERLRRHPDVRAGLVVPRE
                     DAEGRTRLVAYVVVREDAQFDEPDLRRWLASTLPDYMVPFLILPLTELPRTPGGKADR
                     GALPAPDWSGVSRQSYVAPATPLQRQLAVMWSEVLAVGRIGLHDNFFDLGGHSLLAVQ
                     LIARIQKVVDRPLALMELFQFPTLATLAEHLEGERRDTSPEILVLRKGRNAPPLFCFD
                     PTGTHVQAYRPLALSMADERPVYGLSLSHLFTMRWQDVSIHQLAEQQARLIRERQRQG
                     PYHLLGWSNGGVLALAVARLLEQGGDSVAFLGLLDTQPDQAVSAAGDATPVEELMTYI
                     RRDRRGDFESIPQHEREALQQHLASLADDNRLEYAIQWARERNFLSDEEAEASIGSLK
                     LGYALAREAARFVTVTRFNPIQAPIHVWWTTSTLAKHGKGPVDWSAHTTGLVSVDTVV
                     GDHVDAVYSIHAHQRIGDKLAGIRAPSA"
     gene            complement(11769..>16871)
                     /locus_tag="PKK81_05130"
     CDS             complement(11769..>16871)
                     /locus_tag="PKK81_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNV32284.1"
                     /translation="IAWLQRQDQAAAEQFWKQELAGFTTPTPLGIERLDPDTSASGSE
                     VGDEFMELSPEVSERLRTLAQQHHLTPNTFVQGAWAILLSRYSGEAQVLFGVTVAGRP
                     TDLPGVEDIVGLFINTLPLRVTLSPDQSVMTWLQHLLAENYRIRQYEYAPLVRIQQWS
                     EVPSGQALFRSLLVFENAPKDARLGEERDDRSLSYDQDRVHTNYPMTVVGYPGESMGV
                     RLSYDRRLFERADVARMLDHLKRLLEAMAASPEAAIQDLPMLDDAERQRLLVEWNGRD
                     GSEPAAADFSTRFEAQVRRTSEAVAVECGDDRLTYDELNRSANRIAHGLRRRGVGTDS
                     IVALLDDRGPALLSMIVGVLKAGGAYLPLDPHHPAERLTQILASACVKAVIFGNRQAA
                     LAETVATMLPAGVGPDFVSVENLERDAQGESNPSPVTAPQRLAYVIFTSGSTGVPKGA
                     MVESRGMMNHLLTKIPTLALGPADVVAQTASQCFDISVWQFLTPLLCGAKVQIIPDEV
                     VRDPQRLLAEVSARGVSVLEVVPSLLGGLLEGPSLPLPRLRWLLPTGETLSPDLSRRW
                     FARYPTVPLVNAYGPAECSDDVALACIREQPAEAEFRMPIGRPIEGVRLYVLDRRLDL
                     MPAGVAGELCVGGVAVGRGYLSDPAKTAALFVPDPFSSRPGSRLYRTGDLVRHRPDGA
                     LEFVGRRDHQVKIRGFRIELGEIEARLAQHPDVDRCVVVVAEWQPGHKQLVAYVATTA
                     TVNADRLRLFLRRTLPEYMLPATFLLLPSLPLTPNGKIDRKALPTPDADRLADDFEPP
                     ATPTQDLVAGIWSEILGIERIGRRDQFFELGGHSLLATQVMSRVRAAFRIELPLRTLF
                     DHPTVEAFASAIDRAGLEGDGGQIPPLVPVSRTDHLPLSFAQQRLWFLTQMDPESGTY
                     NLPFALRLGGALNHRILERSFFALVRRHETLRTTFPAVEGEPRQVVVDVDRFAIPVED
                     LTALSESERGTAIGRYAEAQAQRPFHLDRDLPIRALLLRVSADAHILLVTVHHIAADA
                     WSLAMVTHEVATIYNALANEVEGAGMQSGDEPETLLPALPVQYRDFAVWQRQWLQGAL
                     LEREIGYWKQRLGSTPPQLMLPTDHARPERQSYRGGRLTFHVPGEILEPLRRLSRRQG
                     VTLFMTLLAAFKLLLSKFSGQTDILVGTDVANRNRAETEQLVGFFVNLLPLRTDLSGN
                     PSFVQLLGRVRETALGAYAHQDVPFEKIVDSLKLTRDLGRNPLVQVLFVLQNVPPPSL
                     QLAGVNVESLEFEHEVSRFDVGLFMEETEDGCAGLWKFSRDLFEPQTIAGWTQRFLRL
                     LQQIGLSPECGIETLEMDDAEVKEARRMDTKQRNDERLQRFKAIKPKPVALAQRTLVS
                     GRPLVDGRAMPWLYTPAVDDVDLAGWVREQRSRLQRELLQSGALLFRGFALKTVQDFE
                     SVAQAFCGELFGDYGDLPREKSGRHVYGSTPYPPDKPILFHNESSHMHRWPQKQFFFC
                     LQAAQEGGQTPIVDGRLMFKGLRTDLRERLREKQLMYVRNFVPGVDVSWQDFFRTSDK
                     AEVEALCAKNGMDWQWLEKGGLRTKQVCPAIIEHPDTGESVFFNQIQLHHVSCLEPAV
                     RDSLLSMLGIDSLPRNVYYGDGTPLEDEVVEEIGLLYERTAVRFPWQEGDLIMLDNML
                     VAHARDPFVGPRKIVVAMGDMIAKSAVHSMTS"
     assembly_gap    16872..16971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16972..>18332)
                     /locus_tag="PKK81_05135"
     CDS             complement(<16972..>18332)
                     /locus_tag="PKK81_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV32285.1"
                     /translation="IVNHYGPTETTVGVLTHLVEASQGHAESDVPIGRPLSFVQAHLL
                     DSDGQPVPVGVLGELYIGGQQVTRGYLGRPEATAERFIPDSFSRRPGARLYRTGDRAK
                     RRADGTVEFLGRIDHQVKLRGFRIELGDIEAQLRREPGVADAVTVVRETGDGTQQLVT
                     YLAGPSMLDVAGIRARLALRLPQYMVPQHFVPLDAFPLTANGKLDRAALPDPEQLSAA
                     ALTTYVAPRNRTEEILAAIWQTVLQVERVGVHDNFFALGGDSIRTLQVIARANRQGVK
                     LTPKQLFEHQTVAAAAAVAQVKPATPIEAMQSPVQDEARPAAGAHSPPQATPEATAPV
                     MPIFPLTGLTPEELAGRREDWAEIEDCYPLSPMQEGMLFHTLMNPGSGIYLMQQFYVW
                     EGCLDREAFGHAWQRVIDRHPMLRTSFMWKDLQAPLQVVHRRVAAEDVIQDLDWADMS
                     DEQ"
BASE COUNT         3517 a         6014 c         5490 g         3211 t
ORIGIN      
        1 acgctccaca tcaccttgac caacggcaag acgggcgact ttccgctggc gatcgacggc
       61 agcttcaaag gcgactcgga tttcggccaa gttgagctgc cggtccacgg tctcaagaag
      121 ctcgagttta agtaaacagc accggggccc cctcccgatc atccgtcggg aaccgcgtga
      181 ggccgaatga acaggagcca ggaccagcag caccgctcat gacgctccgt cgtaccacct
      241 acctcgttat cttcacgctg ctcgcactct ccgcgatgct cgccgtgaaa tggaccgtgc
      301 tgactccggt tcctgcctcg gaagtgacgg gaaccgtgcc ggcagggacg gccgtcgtgc
      361 tcgccccggt caccaccgag gcgatccggc acacgatcaa agccgtcggc tccttgcggg
      421 ccaatgagag catcatgatc cgtccggaga tcgcggggcg aatccgtcag gtctggtttc
      481 aagagggtca ggcggtggag aaagaccagc tgctcatcga aatggacgat tcggaactcc
      541 aggcggaact ggctcaagcc gcggcgcaac tcaaagtcag ccgactcacc tacgaacggc
      601 tcaaacgact cgacctcgac ggaaagcggt acgtgacgaa acagcaactc gatgaagtgg
      661 ccggcgcgct gcaagtctct caagccaacc acgcgctcta tgtcactaga ctggccaaga
      721 cgaaaattcg ggtccccttc accggcgtca ccggcatccg gcgcgtctcc cccggcgact
      781 tcgtcggtgc cggccgggat ctcgtgaacc tggaagatct cgacaccttg aagatcgatt
      841 tcaaggtgcc ggaaaccatg ctgcgccatc tggcaccggg tcagtccctc gaactgacca
      901 cggacgccta tccggggaac acgttccatg gcacggtcta cgtgatcgat cctcaggtcg
      961 acccgagcac gaggtccgtg caggtgcggg cgagaattcc gaatccacag ctgcggctca
     1021 gacccggcat gttcgcgcaa gtgctcctca cgttcggaca ggaagagcgg gcgctgctga
     1081 ttccagaaga ggccgtcatg ccgaaacagg acaaggccta cgtctacctc gcccaagacg
     1141 gaaccgccca acagcgagag gtcacgctgg gcacccgcac caggggcttc gtgcaagtgc
     1201 tggacggact cacggaacag gatacggtga tccgggtcgg ccaccacaag ctgcagaacg
     1261 gcgactccat tctggccctg tcctcgacgc ctcactaacg ataacccatc ggacgtcccc
     1321 atgagactct ccgccctctc cattgagcga cccgtcctcg ccgccgtggc gacgctcctg
     1381 ctgatgctct tcggcctgct ctcctattcc cgcttgccgg tccgcgagta tcccgacacc
     1441 accttcccca ttgtctcggt catgaccgtg tatccgagcg cggacgccca attggtggaa
     1501 acggatatca cctcggtgct ggaggatgcc ctcagcggcg tggaagggct ccggaccttg
     1561 cgctcgacca gtcgtgaggg actgtccacg atcggtctcg aattctcgtt gatgcgcaac
     1621 ctggatgcgg cggccaacga cgtgcgcgac cgcgtgacga gagtgcaata cctgcttccg
     1681 cagggcatcg aagcgccctt ggtgatgaag gaggacagcg actcagacgg catcatgtgg
     1741 ctggcgctga tcagcgaccg ccacaccgag ctggaaatga ccgactttgc cgaacgccag
     1801 ctgaaggatc gactcgcgcc cctgcccggc gtctccagcg tcatgctcga cggaggacgg
     1861 cggtatgcga tgcggatctg gctggatccc gaccgactcg cctcgcggct cctcaccgtg
     1921 caggacgtcg aagacgcgct ccggacacaa aacgtctcca tgcccaccgg ccggatcgaa
     1981 agcacccagc gggagttcag tgtgcgcatg gggggaggcc tggagaaagc ggcgcagttc
     2041 aatcaactga ttctggccta tcgggacggc tatccggtcc ggctgcagga cgtgggtgcc
     2101 gcggagatcg gcccggaaga cgatcgcaaa ctcgtgcgcg tgaacggcaa acccgccatc
     2161 ggcctcggca tcatgaaaca gtcgaaggcg aatacgctcg aagcggcacg cgccgtcaag
     2221 cgcaccatcc cggccatgga ggcgttgctt ccggaaggga tgcagctcgt cacggcgttc
     2281 gacagctcga tctacatcga gcggtcgctg cacgaagtgt acgaggccgt cggcctgtcc
     2341 gtgctgctcg tcgtgctggt agtgttcctg ttcctgcgca gcgcccgcgc cacactcatc
     2401 cccgccgtcg ccattccagg ctccatcctg ggtaccttcg tgatcatgca gatgaccggc
     2461 agctcgatca atacgatcac cctgttggga ttcgtgctgg ccatcggact ggtagtagac
     2521 gacgccatcg tcgtggtgga aaacgtctac cgccgcattg aacgggggtt gagcccgaca
     2581 caagccgcga tcgaaggcag ccgggaaatc gggtttgcgg tcatctcaac aaccctgacg
     2641 cttgcggcgg tcttctttcc gatcattttt ctgcccggta tcgtcggtcg cctgtttgcc
     2701 gaactgggca tcgccgtcgc cggctcggtg ctcctgtccg ggttcatcgc cctcactctc
     2761 acccccgcca tgtgcgcgcg gttgctcgga aagactgcgg gaaccgcctc gccgtcccga
     2821 ggctccgcac tggatccttc ggcttggctc cgctggctca ctcaacgcta tcgcctcgcc
     2881 ctgaaagtcg ccttggaaag gcccgcgatc atcctaggtg gagccgtcct gtcactgatc
     2941 gtcagcgccg ccctcatgtg gggtctcccc agcgaactgg caccacttga agataccgga
     3001 tggtttgccg tccacgtcag tgcgccggaa ggctctactc tcgactacac cgaccgttac
     3061 gccagggaaa ccgagaacgc cacggcacgc attcccgaaa tggattccta ctataccgtg
     3121 gtcgcgcggg gctggaggcc gaccctggtc aaccgggccg taacctgggt gacgctcaag
     3181 gattggtccg atcgcaaccg cgtccagcag gaaatcgtcg atgacgtgca acctgccctc
     3241 tccgccatcc ccggcgccac ggtctttgct ctcaatccgc ccccgttcaa tcaggaggac
     3301 agcaaaacgc cggtgcagtt ggttattcgc ggcccctcgt acgaggtgtt ggacaagatc
     3361 gtgacacaga tccggcatga cctcaccgga aaccaggagg tggtcaatgt cgagagcgat
     3421 ttggatttga acaaaccgga actccgggtg gagatcaacc gcgacaaggc ggcggacctg
     3481 ggcattccga tcgagacgat cggcaggacg ttggaagtgt tcctcggcgg gagaaaggcc
     3541 tccatgttca tgcgcgaagg caaagagtat cccgtgatca tgcaaacccg tcccgatcaa
     3601 cgtggcacca ggtccgatat cgaacacctg caggtacgag gcacccaggg ggatttgatt
     3661 cccttgagca atctcgtcac gctgcgggaa accgtggccc cgaaacagct gaaccattac
     3721 gaaaaactga gggccgtcac gatcagcgcg ggagtggggg ccgattcgac gctcagccgg
     3781 tccatcgaat cactcgaaca gacgatccgc aagcacctgc ctcccggcgc aaccatcagt
     3841 tatgcgggag agacgaaaga gttcaaggag tcgtcgggca atctgcatct cacgtttctc
     3901 ctggccctgc tcgtcgtcta cctcatcctg gccgcgcagt tcgaaagctt ccgccacccg
     3961 atcaccgtcc tcgtctcggt tcccccggcg ctggccggcg cgctgttggc gctgctgctg
     4021 ttccgcggca ccttgaacat ctatagtgag atcgggttga tgatcctgat cggcctcgtc
     4081 accaaaaacg ccatcctcat tgtcgaattt gccaaccagc tgtgccagga gggcaaatct
     4141 ccgcgcgctg ccatcgtcga ggccgccgtg ttgcgcctgc ggcccatcgt catgacgaca
     4201 ttgtccacca ttctggccgc gctgcccctg gcgctggcga cgggcgccgg cgcagccggg
     4261 cgctggcaca tcggcctggt cgtgatcaac gggctcgtcc tctcaacgct cctcaccctc
     4321 ttcctggtgc cggtggtcta cctccttttt gccaggactg ctccggtccg acagtccgca
     4381 gcggtgcctg agccattctc ggaagtcctg ccgcgtcact cgccttcaca aggctgacag
     4441 cgacggccac tgcagggccg gactgatgtc ttcaggataa aatcgtgaaa gttcgggaag
     4501 acggagggtc aaagagtctt ccggcttaat ggccgcaaga acggcaggaa acccgcttcg
     4561 agacagagcc gtcggtgttc gttatgccga cggcgcgcga atgccggcga gcttgtcccc
     4621 gatccgctgg tgtgcgtgga tgctgtagac ggcgtccacg tgatctccga ccaccgtatc
     4681 caccgacacg agacccgttg tatgggccga ccagtcgacc gggcccttgc cgtgtttcgc
     4741 caaggtgctc gtcgtccacc agacgtggat cggcgcctga atcggattga agcgggtcac
     4801 ggtcacgaat cgtgcagcct ccctcgccag ggcataaccg agcttcaaag agccgatcga
     4861 ggcctcagct tcctcatcgg aaagaaagtt tcgttccctc gcccattgga tggcgtactc
     4921 caggcggttg tcgtccgcca gtgacgccag atgctgctgg agcgcctctc gttcgtgctg
     4981 agggatcgac tcgaagtcgc ccctccggtc ccgccgtata taggtcatca actcctcgac
     5041 aggcgttgcg tcaccggccg cagagacggc ctggtccggt tgcgtgtcca acagaccgag
     5101 aaacgcgacc gagtccccgc cctgctccag cagtcgcgcc accgccaagg caaggacccc
     5161 gccgttcgac cagcccagga gatgataagg gccctgccgt tgacgctcac gaatcagccg
     5221 ggcctgttgt tcggcgagct ggtgaatgga gacatcctgc caacgcatgg tgaagagatg
     5281 gctcaacgac aatccataga cgggacgttc atccgccatc gagagcgcga gcgggcggta
     5341 ggcctgaaca tgcgtgccgg tcgggtcaaa gcaaaacagc ggcggcgcgt tgcgtccttt
     5401 gcgcagcacg aggatctccg gcgaggtgtc acgacgctcc ccttcgaggt gctcagccaa
     5461 agtcgccagc gtgggaaact ggaacaactc catcaaggcc agcggtcgat cgacgacttt
     5521 ctggatgcgg gcgatcagct ggacggcgag aagggaatgt ccgcctaaat cgaaaaaatt
     5581 atcatggagg ccgatccggc cgacagccag cacctcgctc cacatcacgg ccagctggcg
     5641 ctgcaacggc gttgcgggag ccacatacga ttgccgactg acgcccgacc agtccggcgc
     5701 aggtaaggcc cctcgatccg ccttgccacc cggtgtccgc ggcagctcgg tcagcgggag
     5761 aatcaggaaa gggaccatgt aatccggcag ggtcgaagcg agccagcgac ggaggtccgg
     5821 ctcgtcgaac tgtgcgtcct ctcgaacgac gacataggcc acaagcctgg tccgtccttc
     5881 ggcatcttcc cgtggaacca cgagaccggc tcgcacgtcc ggatgccggc gcaaccgctc
     5941 ttcgatctcc cccggttcga tacggaaccc gcgcagtttg acttgatcgt caaaacggcc
     6001 atggaattcg atggtgccgt cgctcctgac caatgctcga tctcccgtcc tgtacagtct
     6061 tgcgccgggc cgtcccccga aggggtcggg aatgaacagg ccggcggtcc gttccggatc
     6121 gtcgagatag ccgcgggcca gcgtctcgcc cccgatatac acttcgccgg gcacgtcaaa
     6181 cggcaccggc tcgaggtgcc ggtcgaggac gtacatcgac cggcccggca acggccttcc
     6241 gatcggaacc gtatgaaggg caaattcttc cactgacagc ggaggctcat acaggctcgc
     6301 ggtaatgatc gtttccgtcg gaccataggc attcaaccac cgtacgttgc tgccgacctg
     6361 tcgtcgccat cgattcagat ccgatgatgc caccggctcg ttccccacga tcagcaggcg
     6421 cacacgtgag ggcacggagc aatcggcttg ctcgaacgcg tccacccact ggtgccagta
     6481 agaggccggt aatcctgcga ccgtgacgtg ccggcgatcg agaaagtcca ggaatgccgg
     6541 gatggaatcg aagagacgac agggttgcag caccactgca gcccccgaga tcaaggtcgt
     6601 ccacaattct tctgaggcca cgtcaaagct gacggccgcg aattgcagca cccggtccga
     6661 cggcccgaac ttataggcag ctgcagcggc aacgagatgc tgacacaggg cccgatgtga
     6721 gaccacgacg cctttcggtt tgccggtcga gccggacgtg tagatcacat aggcggcttg
     6781 gtccgccgag atcggcctgt cgaggttctc cccgctcagt gtttccaact ctgcattcag
     6841 tcggtcgagc gacaataccg gaacagggaa tgtatcgggg tcaggcgtgg cggtcccttg
     6901 cgtgaggacc agatccacgc ccgcatcgac cgccagagtg tgttgccgcc cggccgggta
     6961 cgtaggatcg atcggcacat aggcaccgcc cgccttcatg atcccgagta aggcaacgac
     7021 catgtcgaac gaacgctcca gacaaatccc gacccgccgt tccgactcca cgcctcgact
     7081 gcgaagccat cgcgccaggc ggttactctt gtcgttcagg tcccgatagg acaccacatg
     7141 gtcttgcaac accagggccg gcgcatccgg cgtctgcgcc gcccgacgtt cgatgagcgc
     7201 cggcagggac tgcgacgggc cactcgacgc atgcgtccgg ttcaccgtca gcaccgcttg
     7261 tcgctcctgc gcgctcagca gatggatcga gcctactccg attcgcatgt cccccgtcat
     7321 tccggccaac acctgctgat agcggtccaa caggcgttcg atagtcgacg ccacgaagag
     7381 gtcgctgttg tattccatcg cgacgagcag ccggccgtct tgatcctgaa ccgtcatcga
     7441 caggtcaaac ttcgcagtcg tggtgtcgat ctcgatcggc tgaaactcca agccctggat
     7501 gttcaattct gagaccgggg cgttctgcag gtcgaacatc acctgaaaga ccggcgaccg
     7561 gctgacatcg cgcttcggtt gcaatgcatc cacgagcttt tcgaacggca tgtcttgatg
     7621 ggcgtaggcg tccagacagg cttcacgcac gttggccagc acgtcggcca tgctggaatg
     7681 tccgaacacc ttggttcgta tcgccagcgt gttgacgaag aacccgatga ggccttcggt
     7741 ttccttcctg gtccggttgg cgatgggcgt cccgacgatg agatcggtct gtcctgtcat
     7801 gcgggccaac aggatgaaga aggcgctcaa cagcgtcata tacaacgtgg cgcccatttt
     7861 gcggctcaac gccgaaactt tcaccgaaag ctccgccggg atggtgacgc tcgccatcgc
     7921 gcctcgcgat gtttgcaggg caggtctggg cctgtcagtc ggcaattcca tgaccggcag
     7981 atcgccaccc agcgatgtcc gccaaaactc caactcccgc ttgaggacgg tcccgctcaa
     8041 ccaggcacgt tgccactggg aaaaatctcc atactgcaga ggcaggtcgg acagtgacga
     8101 ggcccgcccg gccgcaaagg cgtcgtagag cgccgtcact tccttcacca gcacatgggc
     8161 ggaccaggcg tccgacacga tatggtgcaa cgtgagcaac aacacctgtt cgttcggctg
     8221 gaggcggatg agactgaccc gcaggagcgg cccgtatcgg agatcgaatg gacgctggga
     8281 ctcgacgccc gccaaccgga cggcagcctg gtcacgcgct tcgacaggaa gatgttgcag
     8341 gtcgatcacc gtcacggtca gcgtcagaga ggaagcgacc acctgcaccg gcactccatt
     8401 gagactttga aacgtcgtac gcaacgattc atggcgccgg acgacttcgt tgaaactccg
     8461 ttccagggct gcgacatcga gcggtccggt gacgcgcaag gcgatgggaa tgttgtaggc
     8521 cacgccctcc ggatccaact gcgccaacac ccacaggcgt tcttgtgcga aggaaagcgg
     8581 gagtgaacag gctcgcggcc agggctgcat gggaggcggt tggtcgcccc ccgcgatccc
     8641 cttggccgcg tcgatcaccc gggctaattg cgcgatgttg ggcgcctcaa agactgtccg
     8701 cagaggcaga tccacgcgaa acgcctgccg cagccgcgac atgacctgcg tggccaggag
     8761 cgaatggccg cccaagtcga agaaattgtc atggatgccg atcccctctc gtttcaacac
     8821 ctcactccag atggcggcca ggatctcttc cgtggccgtt ccgggagcca cgaaccgtat
     8881 cgtttcgctc ccctcgcggt ccggctccgg tagggcgcgc cgatcgacct tgccgtttgc
     8941 cgtcaacggc aacgccggca gagagctcac cgccgtcgga accatgtagt ccggcaggtg
     9001 cgagcggaga aagtcctgga tcgcctgcct gtccggctcc gccccccgcg ccacgaggta
     9061 tgcagccaga tacagctccg cattcccgtc ctcccgtgcc gtcaccacgg catcctgtac
     9121 cgcaggatgc tgtcgcagca ccgattcgat ttcgccgagt tcgatccgaa acccgcggat
     9181 cttgacctgg tggtccaccc gccccaggaa ttcgatcgag ccgtccggca aacgcctcgc
     9241 gaggtcgccg gttcggtaca ggcgtgcgcc gggttgcgac gcatgggggt tgggcaggaa
     9301 ccgttcggcc gtgcgatccg gttgctccca gtagccgcgc gcgacacccg caccgcccag
     9361 atacagttct cccgctgcgc cgacgggcac cggctcctga cgctcgtcga ggatgtaggc
     9421 ttcgaggttg tcgatgggac ggccgatggg tactgtgcga gcgagacgta catcctcgtt
     9481 gtcgcccaca accgtggtca aagcgccgac ggtggtctct gtcgggccat aatggttgat
     9541 gacgatgcag gccgcttgcc gccgcacctg ctcgaccaag tcccacgaca gcgcttcacc
     9601 gccgaacagg agaaactttc gtggccacaa ggcctgcccg tcggcagtgg ccacgagggc
     9661 cttgaaatgc gacggcacga ttttcaacac gtcgatggga tgctccgaga gataggaggc
     9721 gaataaccga ccatccgtcg cggtctcgta gttgatgaca tggagacaac cgccggaggt
     9781 cagggcggga aaaatcaccg tatgtcccaa atcggcgctc aaggtggaga cggtggcaaa
     9841 ctgcaagggt tcccgaagat ccagctgtcg caatatggcc aaggtgtagt tggcgaggtt
     9901 acgatgggag acggcaacac ccttgggctg accggtcgag ccggacgtgt agataacgta
     9961 agccaggtcg tccggcgaaa gcgccagatt gagattgccg tcaggctcgt tcgcaacagc
    10021 ctcgccgacg tccaagatca cgccgtcgaa gagcggccgg gcctgctcgg atacactctg
    10081 cgtcacgaga agcctggcgc cgctttgcgc catttgcggc gcgagccgca cagccgcatg
    10141 cgccggatcg atcggcacat aggctgcacc ggccttcagc acccccaaca tggcgacgat
    10201 catgtcggca gaccgatcga gacagaggcc gacccggtcg ccgggttgca cgccgcgccg
    10261 cctcagccca tgcgccacct ggttcgcctg ccggttcagg tcgtcgtagg acaaccgtcg
    10321 ctcaccggcg acgagggcgg gagatgacgg ccgccgcgct gcctgcgctt cgattaaacg
    10381 gtgcatcgga aaagaagggc ctagcggtct ggccgtctgg ttcaactctc gcgtcagcct
    10441 ggtccgctcc cgagtcccta cgatgtccaa gtcgtcgatc gccgtcgcga gacggcctgc
    10501 aatggcgcgc aacaactcca cataacgatc ggccagggac tggaccatct gggaggggat
    10561 gcgttccccg tcgatatgcc aggtcaatgc gagatccgcc tcgtcttcaa gacaggctag
    10621 cctgaatttg aacggctccg cccagaatgt gctctcggcg agtgtgatga cgccggcggc
    10681 cgtccggctg ggcttcgctc gacgaccata ttcgaatccg atcccctccc gcacagccgc
    10741 cgcgtcctca tcgtcctgtt ccgccgggta atagtgttgc caatcctccg cttgcccctg
    10801 ctcctgcgca gccaaggaca acaggtcccg cagcgccata ttcccctcga ttcgaaggtg
    10861 cagcggcaac cagcgctcga acaacccgat gcctccctgc aactcctcat aggggcgccc
    10921 atcggctcgc caccagcaga ccaggtcgcg acggcccgtc agccgataaa gaagcgccga
    10981 ccaacaggca tacagaagga ccgctaggga aacctgcgcc gcggcggcag cgccccgcaa
    11041 tcgctccgtc tccgtagacg agcagatacg atggaccggg cgagccgcgg cctgccggcc
    11101 ttccggacgg cccgcctgtt cgaagggcaa cggcacggcg tggtcaccgg ttggtcttcg
    11161 tcgagcccac caggcctgcc cttgtttcgc ctcttctcca tccagcagct cataatgcca
    11221 ctcggaaaat tgtccatact gaacaggctg ctcatccttc cagtcggcgc tcactccatc
    11281 caacaactga ctcagttctg ccgccagatt cgacagggac caggtgtcgg cgcagagacc
    11341 gggcaggtcg agaaccaacc gcgcgtggtc gtccgtcaat cggcacaggg tggcccgtac
    11401 caacggcgtc gcctcccaat cccggtcgtc cgttcgacgc gccgcttcac cggcagcctg
    11461 ttcgcgttcg ccctcgtcag gcagatgacg aaggtcgacc cgtcgccaga gaacgtcgct
    11521 ctcctcgttg atcacctgga agggaacctt catgccggat tcccgataga acgtcgtgcg
    11581 aagactctcg tggcgggcga tcgcgtgacg cagcgccttc tgcaccgcct catcgtcgaa
    11641 gacaccgtcc agcctgaaca aagctcgcgc ctgcccgaca tgaccgcgtg actgccacaa
    11701 ccagagtcgt ttctggtgcg gcgagagccg gagcccttca atctcaatgt cctgatgcat
    11761 gcctccccct atgacgtcat ggagtgaacg gccgatttcg cgatcatgtc acccatcgcc
    11821 acgacgatct tgcggggtcc cacgaaagga tcgcgggcat gggccaccag catgttgtcg
    11881 agcatgatga gatccccctc ctgccaggga aaccgcacgg cagtccgttc gtacaacagg
    11941 ccgatctcct cgacgacctc gtcttcgagc ggagtgccgt caccgtaata gacgtttcgc
    12001 gggagggaat cgataccgag catcgacaag agcgagtcac gcaccgccgg ctccaaacag
    12061 gaaacatggt ggagctggat ctggttgaag aagaccgatt cgccggtgtc gggatgttcg
    12121 atgatcgccg gacagacctg cttggtgcgc aggccgcctt tttcgagcca ttgccagtcc
    12181 atgccgttct tggcacacag ggcttccact tcggccttgt cggaggtgcg aaagaaatcc
    12241 tgccaactca catccacacc cggaacaaag ttgcggacgt acatcagctg cttctcgcgc
    12301 aaccgctcgc gcaggtccgt ccggagcccc ttgaacatca accggccgtc cacgatcggc
    12361 gtctgccccc cttcctgcgc ggcctgcagg caaaagaaaa actgcttctg cggccagcga
    12421 tgcatgtggg agctttcgtt gtggaagaga atcggtttgt ccggcggata gggtgtcgat
    12481 ccgtagacat ggcgcccgct cttttcccgc ggcagatcgc cgtagtcacc gaacaactcg
    12541 ccgcagaagg cctgcgcgac ggactcgaaa tcctgcacgg tcttcagcgc gaacccgcgg
    12601 aacagcaagg cgccggactg cagcagttcg cgctgcagtc gcgagcgctg ttcccggacc
    12661 catccggcca ggtcgacatc gtccaccgcc ggtgtataga gccacggcat cgcacggccg
    12721 tccacgagcg gccgaccact caccaacgtg cgctgggcca gcgcgaccgg tttcggtttg
    12781 atggccttga accgttgcag tcgctcgtcg tttcgctgtt tcgtatccat cctgcgcgcc
    12841 tccttgacct ccgcatcatc catctcaagg gtctcgatcc cgcattccgg cgagagaccg
    12901 atctgctgca acaatcgaag aaaccgctgc gtccatccgg cgatcgtctg gggctcgaac
    12961 aggtcacggc tgaacttcca cagcccggcg cagccatcct ccgtttcttc catgaagagc
    13021 cccacgtcga accgcgacac ctcgtgctcg aactccagcg actcgacatt cacgccggcc
    13081 aactgcagcg agggcggcgg cacgttttgc agcacgaaca gaacctggac cagcgggttc
    13141 cggccgagat cccgcgtgag cttgagggaa tccacgatct tttcgaacgg cacatcctga
    13201 tgggcgtagg cgcccaaggc tgtctcccgc acccgcccca ggagctgaac gaaggacggg
    13261 ttgccgctga gatccgtgcg caggggcaag aggttgacga agaaccccac gagttgttcc
    13321 gtctcggcgc ggttccggtt ggccacatcc gtaccgacca gaatatcggt ctgtcccgaa
    13381 aacttggaca agagcagctt gaaggcagcc agcagcgtca taaacagcgt cacaccctga
    13441 cgccggctca accgccgcag cggttcaaga atttctcccg gcacgtggaa cgtgagacgg
    13501 ccgccccgat aactctgccg ctcaggccgc gcatggtcgg tcggcagcat caattgcggc
    13561 ggcgtggaac caagccgctg cttccagtag ccgatctccc gttccaacag ggccccctgc
    13621 agccattgcc gctgccagac cgcgaagtcg cgatactgga ccggcaaggc gggcagcagg
    13681 gtttccggct catccccgga ttgcatgccg gctccctcga cctcattcgc cagggcgttg
    13741 tagatcgtcg ccacctcgtg agtcaccatc gcgagcgacc aggcatcggc cgcaatgtgg
    13801 tgaaccgtca cgagcaggat atgggcgtcg gcactcaccc tgagcaggag cgcgcggatc
    13861 ggcaggtcac gatccaggtg aaacggccgc tgggcttgag cttcggcata gcgaccgata
    13921 gccgtgcccc gctccgactc cgacagggcc gtgaggtcct cgaccggaat cgcaaagcgg
    13981 tcgacatcga cgaccacctg cctcggctcc ccctccacag ccgggaaggt ggtgcgcagg
    14041 gtttcgtgtc ggcgcacgag cgcgaaaaaa cttcgttcca ggattcgatg attgagcgct
    14101 ccgccaagac gaagggcgaa cggcagattg tacgtcccgc tctccgggtc catttgcgtc
    14161 aggaaccaga ggcgttgctg cgcgaaggag agcggcagat gatcggtccg cgagaccggc
    14221 acgagcgggg gaatctgtcc gccgtcccct tcgagcccgg cccggtcgat ggccgaggca
    14281 aaggcctcga ccgtcggatg gtcgaacagc gttcgcaagg gcagttcgat gcgaaacgcc
    14341 gcgcgcacgc gggacatgac ttgcgtggcg agaagcgaat ggccgccgag ttcaaaaaac
    14401 tgatcccgcc gcccgatgcg ttcaatcccc agaatttccg accagattcc ggctacgagg
    14461 tcttgcgtcg gcgtcgcggg cggttcgaaa tcatcggcca gccggtccgc gtccggtgtg
    14521 ggcaacgcct tccggtcgat ttttccattc ggtgtgagcg gtaaggaggg aagcaacagg
    14581 aaggtggccg gcagcatgta ctcaggcagg gtccgtcgca ggaacagccg cagccggtcg
    14641 gcgttcacgg tcgccgtcgt cgctacatag gccactaact gcttgtgtcc cggttgccac
    14701 tcagccacga cgaccacaca acggtccacg tcgggatgct gcgccagtct cgcttcgatc
    14761 tcgcccagtt cgatgcggaa cccgcgtatc ttgacctggt gatcacgacg tcccacgaat
    14821 tcgagcgccc cgtccggcct gtgacggacc agatctcccg tccgatagag ccggctccca
    14881 ggccgggatg aaaatggatc cgggacgaac agagcggccg tcttcgccgg gtcactcagg
    14941 tatccacgac ccaccgccac tccgcccacg cacaactctc cggccactcc ggccggcatg
    15001 aggtccagac ggcgatccag gacatacaac cgcaccccct cgatcggccg cccgatcggc
    15061 atcctaaatt cagcctcggc aggctgctcg cgaatgcagg caagcgccac atcgtcggaa
    15121 cattccgccg gaccgtaggc gttcacgagc ggcacggtcg ggtagcgggc gaaccatcga
    15181 cgcgacaggt ccggtgacag cgtctctccc gtgggcagca accaccgcag tcgcggcagg
    15241 ggcaacgacg ggccctccaa gaggccgccc aacaacgaag gcaccacctc aagcaccgac
    15301 acgccgcgag ccgagacctc agccaacagg cgttgcggat cgcgcaccac ctcgtcggga
    15361 atgatctgga ccttggcgcc gcagagcagc ggggtcagga attgccacac ggaaatatcg
    15421 aaacattgtg aagcggtctg agcgactaca tcggcgggac caagcgcgag tgtcggaatc
    15481 ttcgtcaaga ggtgattcat catcccccgg gattcgacca tcgccccctt gggcaccccg
    15541 gtcgacccgg acgtgaagat gacataggcg agacgttgcg gtgccgtcac tggactagga
    15601 ttgctctccc cttgcgcatc cctctcgagg ttttcaactg aaacgaaatc cggtccgaca
    15661 cccgccggca gcatggtcgc gaccgtctca gccaaggccg cctgccggtt cccgaatatc
    15721 acagccttca cgcaggcgga cgccaggatc tgcgtcagcc gctcagccgg gtgatgcgga
    15781 tccaacggca gataggcacc accggccttc agcacgccca cgatcatcga cagcagcgcc
    15841 ggcccccggt cgtccagcag cgcaacgatg ctgtccgttc ccacgccgcg cctgcggagt
    15901 ccatgggcaa tccgattggc ggagcggttg agttcgtcgt aggtgagacg gtcgtcgccg
    15961 cattccaccg caacggcctc cgaggttcgt cgcacttggg cttcgaaccg ggtggaaaaa
    16021 tccgcggccg ccggctcact gccatctcgc ccgttccact ccaccagcag acgttgtcgc
    16081 tcggcgtcat ccagcatcgg caggtcttgg atcgccgcct cgggagacgc cgccatggcc
    16141 tcgagcaatc gcttgagatg gtccagcatg cgggccacat ccgcgcgctc gaagagccgt
    16201 cggtcatacg acaggcgcac tcccatggac tcgcccggat acccgaccac ggtcattggg
    16261 taattcgtgt gaacgcggtc ctggtcgtac gagagggacc gatcgtcccg ttcttcgccg
    16321 agacgcgcgt ccttcggggc attctcgaac acgagcagac tgcggaagag cgcctgtccg
    16381 gacggaacct cactccattg ttgaatgcgc accagcggcg cgtattcgta ctgccggatg
    16441 cgataatttt ccgccaacag gtgctgaagc cacgtcatca ccgactgatc gggcgacagg
    16501 gtcacgcgaa gcggcagggt attgatgaag agccctacga tgtcctcgac accgggcagg
    16561 tcggtcggtc tcccggcgac ggtcacgccg aacaacacct gtgcctcccc gctgtagcgc
    16621 gacagcagaa tggcccaggc tccctgtacg aaggtgttcg gggtcaggtg atgctgctga
    16681 gccaaggttc gcagccgctc ggacacctcc ggtgataact ccatgaactc gtcgcccact
    16741 tcggacccgc ttgcagaggt atcggggtcg agccgttcga tccccagcgg ggtcggcgta
    16801 gtgaaaccgg ccaactcttg tttccagaat tgttccgccg cagcctggtc ctgacgttgc
    16861 agccaggcga tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgctgctcg
    16981 tcactcatgt ccgcccaatc caggtcttga atcacgtcct ccgcagcgac ccgtcgatgg
    17041 acgacctgaa ggggcgcctg caaatccttc cacatgaaag aggtgcggag catcgggtgc
    17101 cggtcgatga cgcgttgcca ggcgtggccg aaggcctccc gatccagaca cccttcccag
    17161 acgtaaaact gctgcatgag gtagatgccg gaaccggggt tcatcaacgt atggaacagc
    17221 atgccttcct gcatcggcga cagcgggtaa cagtcttcga tctccgccca atcctcacgt
    17281 cgacccgcca gctcctccgg cgtcagcccg gtgagcggga agatcggcat gacgggcgcc
    17341 gtggcctccg gcgtcgcctg tggtgggctg tgagcgcctg ccgccggccg ggcctcgtct
    17401 tgcacgggcg attgcatggc ttcgatcggc gtggccggtt tcacctgcgc caccgccgcg
    17461 gcggccgcca cggtttgatg ttcgaagagt tgcttgggcg tcagtttcac cccttgccgg
    17521 ttggctcggg caatgacctg cagcgtgcgg atcgagtcgc ctcccagcgc gaaaaagttg
    17581 tcgtgcacgc ccactcgttc gacctgcaac acggtttgcc agatggcggc gaggatttcc
    17641 tcggtcctgt tgcgcggcgc cacgtaggtc gtcagcgcgg cagcgctcag ctgttccgga
    17701 tcgggcaagg cagcccggtc gagcttgccg ttcgcggtca gaggaaaggc gtcgagaggg
    17761 acaaagtgtt gcggcaccat gtactgaggg aggcgaagcg caagcctggc ccgaatgccg
    17821 gccacatcca gcatcgaggg tccggccaga taggttacga gttgctgcgt gccgtcgccg
    17881 gtctcgcgga ccaccgtgac ggcatcggcc acccccggct cccgtcgcag ttgcgcctcg
    17941 atgtcaccca gttcgatgcg aaacccgcgc agcttgacct gatgatcgat gcgtcccaga
    18001 aattcaaccg tgccgtcggc tcgccgtttc gcccggtccc ccgtacggta caacctggct
    18061 ccaggccgac ggctgaagga atcgggaatg aaacgctccg ccgtcgcctc gggtcggccc
    18121 agatacccac gcgtgacttg ctgcccgccg atgtagagtt ctcccagcac accgaccgga
    18181 accggttgtc catcgctgtc gagcagatgg gcctggacga aggagagtgg acgaccgatc
    18241 ggcacgtcgc tctcggcatg gccctgtgat gcttcaacca gatgggtcaa cacgccgacc
    18301 gtcgtttcgg tcgggccgta gtgattgacg at
//
LOCUS       DAOQRM010000039        18044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2217_length_18044_cov_21.489410, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000039 DAOQRM010000000
VERSION     DAOQRM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18044
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2217_length_18044_cov_21.489410"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PKK81_05140"
     CDS             complement(<1..533)
                     /locus_tag="PKK81_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV32286.1"
                     /translation="MTTPASPDDPLTLATILEEETELLHGPLPKDHPVGAPDAVRTAA
                     LFRHIHACHPKRAGLCFSGGGIRSATFGLGVLQSLARLQLLNKFDYLSTVSGGGYIGS
                     WLTAWIHRHPQGLDGVIEDLRVTPKTGATEVPPPVQWLRNYSNYLSPHLGFLSADSWT
                     LFGIYLRNLHLNWMVLLP"
     gene            complement(803..1360)
                     /locus_tag="PKK81_05145"
     CDS             complement(803..1360)
                     /locus_tag="PKK81_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNV32287.1"
                     /translation="MSHPSAPHTPSPPSTAAHRVEQLFEILIFGSRWIQAPLYGGLII
                     AELLYAAKFLTELWHMIVHFREETETLFMLGVLSLIDVTMVANLLTMVIIGGYATFVS
                     KLDLETHPDRPEWLTHVDPGTIKIKLAASLVGISSIHLLKAFVDVTNENPEHIKWKIF
                     IHMTFLGSAIFLAYTDKLMQRDRKH"
     gene            complement(1453..4311)
                     /locus_tag="PKK81_05150"
     CDS             complement(1453..4311)
                     /locus_tag="PKK81_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV32288.1"
                     /translation="MAVHHLPIRRRQDNALDPSILNVLPANIAVLDAKGTILAVNESW
                     ERFAAANGFTGEGYGVGLNYLHICDAAGPQPDAQHVAQGIRHILDGTIRAFDYEYESS
                     SPTLRRWFRVIVTPKQGSPSYGAVVMHINVSDLKQAEAAMHQSEARLRAIVDTAVDGI
                     VVINERGIIESLNPAVTRMFGYTPDQLLGQNVKLLMPDPYHTEHDRYLTNYLQSGQKK
                     VIGIGREVLGLRHDGVSFPIELAVSEMLVDGVRKFTGIVRDISSRKQAEAQFRQVVES
                     APNGMLMIDPLGNITLVNKQVETMFGYSREEMLGKPVEMLIPERFCPDHPAHRIGYFS
                     APSSRAMGAGRELFGLHKNGQEFPVELGLNPIDTPNGKLALASIVDITMRKRTESALS
                     KAAQDLERKNWEISEARDQAVRAGQAKTDFLATMSHEIRTPMNGVIGMTTLLLDTELT
                     AEQKGYLETLKHSGESLLRIINDILDYSKIEAGKFTIEHIPFDLRLTIEDTLDILAPT
                     AQGRQLELVGLIDAQTPRTVIGDPGRIRQILTNLVGNAIKFTEKGEVLIQVMQTDESS
                     GSVTLRFEVIDTGIGLTQEAQAKMFQAFSQADSSTARKYGGTGLGLSICKRLVELMDG
                     EIGLQSMPGLGTRISFTVKFSTPEEGTAPITPPLPVKNLSGLRVCLVDDNATNRSLLQ
                     YHVSAWNMHHESAVDGPSALNLLRQAAQEGTPFDIAIVDVHMPEMSGLELCRLIKEDP
                     AIAKTHVILLTASGQRGDSMLAKEAGAAAYLTKPIRERHLADCMRLTLGREGREEKAQ
                     LITRHTLTEVKAHTVQRVLVVDDNPINQRVAVKMLEKLGCRVDLAGTGMEALAAICRH
                     PYPLVFMDCQMPELDGFETTRLIRSQEQPGTRLPIIAMTANAMAGDREACLKAGMDDF
                     ISKPIIAADLQAKVAHWLPDVRPEDLKPSVANDAPAGG"
     gene            complement(4328..9160)
                     /locus_tag="PKK81_05155"
     CDS             complement(4328..9160)
                     /locus_tag="PKK81_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV32289.1"
                     /translation="MHHLRSFQLRDMTACGAALRRLGTDATSLEQVADRLVRHLYESL
                     TMGHLREPACSLVRLFKTTPYSRLTPDLRALADARLGDTPPPPSLTCLTLLASAGAVP
                     GWNDPARSSRFRVIPLDTLEAVERLPMFSQLFRQLGVSLPSLTQPGPSVLLDQHEQSF
                     NVFHIPEAEGSPYVPGQEEFVLKYGIRSVLGFGAPLPDGELFSIILFSKDFIPESTAT
                     LFKPLALCAQIALAPYATTTAAFHPHHTSKPEQAGGDPTPVHDAHLQARIADLERLLA
                     VHEQTVDEQADRMELIVQGSQMGTWDWEIPTGRVTFNERWASMLGYRLDELEPHVRTW
                     EQLVHPDDLHQVMATVSSHLRGETPTYSSEHRLRTKSGAWCWVFDSGRVVKRDAKGAP
                     LRAAGIHLDISDRKALEAAQARAQCDLQAKQQALDEAQTLAHLGSWHWNIATGQEAWS
                     EEQCRIFGHTPDQSLPTYATFLSALHPDDVERVQQAVDAALKYDSPYNVDCRIRRPDG
                     EVRHVNCRGVVQRDPDGRPVSMAGTVLDITDYKRAEFAWRDSETRMRSIFESAIEGIV
                     VIDERGRIESANTALLNLLGYQAHELVGQNISLLMPSPYREHHDEYLANYLVTGKRTI
                     IGSGRDLPAVRKDGTRIDVHVSVSEMQIGTEKKYTGMLRDISERKRMEETLRESEERF
                     RQLAEHIDAVFWLTSPDKSEVLYVSPAFEAIWELPRDLLYVNPSFWLDHIHPEDRKRV
                     ATAAACQEHLPYDEEYRIVTPSGQVRWIRDRSFAIKNAQGETYRIAGIAVDITAAKQM
                     EAQIRASELRHRALVELSPHAIFVNCEDRIVFANQACAKLLGAIAPSQLFGKTVLEFI
                     HPESHPEVRQRIAKIRHTNRPVPPTEERFVGLAGSIIDVEVAAAPIMFEGKPAIQVIA
                     TDIQTRKDLERALLATNLQLQTILSSATNVSIVATNTKGTITTFNTGAEELLGYSAGE
                     MIGQQSLTLLHVPEEIDRHARELSDLYERPIHGVAALIENAKRGGFDEREWTYQRKDG
                     SRLTVLLTITALRDSDGVVTGFLAIGKDITSRKAAEHALAQARDEALRAAQAKADFLA
                     TMSHEIRTPMNAIIGMTGLLLDTALTKEQLEFADSVRRSSDSLLTLLNDILDFSKIEA
                     GKLHFEELPFDLRMTVEDTVELLAEQAQSKGLELIGLVDAAVPTAVVGDPGRLRQILV
                     NLVGNAIKFTSAGEVFLHVTRETQDGVGLLRFTIKDTGIGIPEAAQGRLFNAFVQADS
                     STTRRFGGTGLGLAICQRLVSQMHGRIGVESHPGQGSTFWFTVQFPETTLAASPSPFS
                     WSRLHGRRILLVDHCDTVRQAMRQELTSHGMTCTVAQSGPEAVELARTAAAAQNPFDL
                     ALIELHLSDMDGFETAALLKQDPATAGVHLVILTTVGRRGDGQTARSIGIDAYLTKPL
                     RQTQLLECLCLLLSPETAAGTPSNAEPPPLITRHTLSESQAGPKPRLLLAEDNPVNQK
                     VACKMLEKLGYRVDVAGNGQEAVAAHERARYPLIFMDCQMPEVDGFEATALIRKMEGR
                     SAHTPIVAMTANAMQGDRERCLAAGMDDYIAKPVRPKELQAVLDTWLGTAGAATGTTG
                     "
     gene            complement(9265..9921)
                     /locus_tag="PKK81_05160"
     CDS             complement(9265..9921)
                     /locus_tag="PKK81_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV32290.1"
                     /translation="MNELQNTPARVFIINPQELVRVGMRTLLNSVPDFMVVGEASSKE
                     EALPLVLHHKPDIVVLDLRVQDGTGIETAKDILAHLPETRVLFLADALNDSTLLSAVA
                     TGAHGYVLQEAGADTLLHAMRSLTKGQSYLDPGVTRHTFAYLRKVADREPERGRHLLS
                     PQERRLLPLIAQGKTNKEIAAELGLSDKTVKNYLANVYSKLHLSRRSQAAAFYLKTIP
                     "
     gene            complement(10167..11492)
                     /locus_tag="PKK81_05165"
     CDS             complement(10167..11492)
                     /locus_tag="PKK81_05165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNV32291.1"
                     /translation="MTPQTSRRVVITGMGVISPLGCTVDLFWHLLSKGESAVKPVSSF
                     DTSPFQACLAAEVRDFDPEDFLHRKQARRMGRATQFAVASAMMAARDSGVELEQEDRG
                     SIGISIGTSIGGMKEAFEFHDAAKLNAYERVNPFTMGMTFPNAISSEVAIVLGLHGPC
                     ETYSIGCSSTANAIGRAYEWIKSGQSSLVVAGGTEAPLHPSVYAAMNAGRALAPDERG
                     AIRNLPRPFDKTRCGMVLGEGAGCLILEDYEHARARGAKMYAELEGWGFTCDAHSMVK
                     ADHTGHEQQRAARLALSTAHWFPEEVDYVNACGLGTMELDALETNTVKQVLGDHAYRV
                     PVSSFKAALGHAFAASGAFQVIGTAKAMEHQFIPPTLNLTTPDPTCDLDYVSGQGRAA
                     HLDRALINSFGFGGKNIVLALSRVNAGIATTNPMAASPGYSMTHLVGVS"
     gene            complement(11534..12805)
                     /locus_tag="PKK81_05170"
     CDS             complement(11534..12805)
                     /locus_tag="PKK81_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32292.1"
                     /translation="MKQLAAQPAAMDATCTLLDLLQQRKTRRFGRGMTLPGGPLQYTS
                     QHEPIPLSREEERYLIYAAIGRSGRNLGDMQFVGRPGASAGQGHALMNFNSRTVPSPC
                     SAQSTQLFYTNDDGVFFVADAAGPDHPWDLEVVQLQSSRLEIPREAPFMLPFNQWYTN
                     RPGTTLFMPVTNIALLYLNLLLTMFSEDTGYFIVDTDNGNAACGLDAFRKSAGGHLHD
                     NLASRKMFSLRELDAGICETAVQEQGIICEHLSLMQQALGLGGGIQSVGSGRHLLGME
                     PHIYPGLGFHFVVPPGKPLRANPVGIPQVWEGPTPPFVPSMKDAVTSLVASKFGPNGT
                     YGKPSEQPWTNPKAAQQVPQHSERAIDATIAFADYVLGTYGRFPAHADACKSIVACQT
                     HHLDEEFYATFYPDSTLPDAHREHMHVWHSH"
     gene            complement(13027..13824)
                     /locus_tag="PKK81_05175"
     CDS             complement(13027..13824)
                     /locus_tag="PKK81_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNV32293.1"
                     /translation="MSQPAFSAKEFQRLGEIMHDARSVDRSDRVWQLVSAVQKVIPYE
                     FSGCGAVDLLRGIDPSLGHSTYPREFCHLYMGQGLAVDPAVNRLITSGQTVTSSADEP
                     TANEPKEIISLKLDFGIKTCLSAGVRGANGSCSYFAFSNFDEKQADKLRLLLDILTPH
                     FHLSYMRCHSAWNPERSAPPPTLLSKREEEILRWVAAGKTNWEISVILKVSLNTVKFH
                     LKNVFQKIGVENRWSAIAYWQTGEQHRIVPSAPPSDDRPPSGANTPD"
     gene            complement(14078..15058)
                     /locus_tag="PKK81_05180"
     CDS             complement(14078..15058)
                     /locus_tag="PKK81_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32294.1"
                     /translation="MRRLAVSALLLLCTLALPLPGSADTASEHVEWGAWSFDYEVRDN
                     SGLALRNVTYGGEQVLAKASMPVLRVKYVKERIWWNPFTWFGSRADSGRCGPFQDRLR
                     WQDLVPIVNCGDQKVCVESSTVKGMKWLELGIYARIGEYHIYQSWHLSEDGELRPVVQ
                     SRGLSCNTDHVHHPYWRFDFDINGNGMDQVFVRDDGGPDSGWGPGWKKYTNERNDVKH
                     TATHRAWFIRDQLTGHGVWVLPGDGYAPLKDDGERDSFADLDVAIRRANADEDLSWVF
                     GARGQLGYDEDNQGVQEQDVVFWYVAHLPHMAALGPTKWLTLGPTLKIHR"
     gene            complement(15077..15862)
                     /locus_tag="PKK81_05185"
     CDS             complement(15077..15862)
                     /locus_tag="PKK81_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32295.1"
                     /translation="MGTRPVMRTSAALMLLLLATGCSLDAASRGPFGPDDPETAVPKK
                     PLPAQLPTRHVLEFSSDESPEHIKPKMAGSALDAGVSRSPNREAVLSAADIAGLRKVA
                     ETDPRVAALLGERWGFIDADRVPPRGKVSFGCCRTSARFTRLTYFSYSHNVAVHVHMK
                     DSTILEASRAEGYLPPEGPRDIQRGIELARADRRLADKVQGMQGHGLLMQPDRGFFRN
                     DPGYGHRVIWITFSPGLGGDPKYWAQVDLTEDRVLDAGNEPPR"
     gene            complement(15868..16389)
                     /locus_tag="PKK81_05190"
     CDS             complement(15868..16389)
                     /locus_tag="PKK81_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32296.1"
                     /translation="MNRSIEGVVTFLQDNDSLIKQLMETAESATNEIEQLADRVGNAR
                     EEAQVLVDQLGAVEFSVGRQDDTKVRQAALLEAVAALRKKFETYKNDPLRRGIYAAEI
                     SNLYLQLANTFNNETVAQIVPFGADEISGYKDLMKKAVLDAASRKKRAAVIKAGVQIS
                     RLALGVAGKLALV"
     gene            complement(16400..17110)
                     /locus_tag="PKK81_05195"
     CDS             complement(16400..17110)
                     /locus_tag="PKK81_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32297.1"
                     /translation="MVTRGVRLLSVVILFLMATGCHSIHSRSVRDLIQLEGTKIDAAQ
                     TNIDLFQKETEARIKFLEQARSSLHESFKSLQIQETKHRFALASFRNLTTKKGDSAYA
                     AAYLTSLIYMADAQGLEKAVWNQFEEDFCGLRDTANALNDSWKRTATLHAQVHRFAKQ
                     TAFASVDPEFVTAVLDQAPNRSGQIMEILDHSRTVNDALEEVVGARILRIGGLDRAQL
                     FTADLVELLDRLKKDDGQ"
     gene            complement(17146..17778)
                     /locus_tag="PKK81_05200"
     CDS             complement(17146..17778)
                     /locus_tag="PKK81_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32298.1"
                     /translation="MRMLRIVHILIFASLAACTTPPEVKQALTAKDQGYTENEQLMQQ
                     YRELVGNINSRHVQWFRYVQTRLKLDLALQWATTNPRLTDIPDATLADDDAEVLGPDV
                     LAVINAMRLKSLPERKGANGQPVFLAGSGDMSHLIQQIPELISRIEQRVASDSKAPQA
                     LDLTAFDQYRNNVEALRRINGIIKQYLDIDVTLSRNDTQSLADALRTVRR"
BASE COUNT         3259 a         5149 c         5860 g         3776 t
ORIGIN      
        1 ggcagcagca ccatccagtt caggtgcagg ttgcgcaagt aaatgccgaa gagcgtccat
       61 gagtcggccg agaggaatcc caggtgcgga ctcaggtaat tgctatagtt ccgcagccat
      121 tggaccggcg gcggcacttc ggtggcgcct gttttcggcg tgacccgcag atcctcgatg
      181 acaccgtcca atccctgcgg atggcgatgg atccaggcgg tgagccagct gccgatgtag
      241 cctccgccgg agacggtcga aaggtagtcg aacttgttca gaagctgcag gcgcgcgaga
      301 gactgcaaga cgccgagtcc gaaggtcgcg ctgcgtatgc ctccgccgga gaagcagagt
      361 cccgcgcgtt tcgggtggca ggcgtggatg tgccggaaga gcgcggccgt gcgcaccgca
      421 tcgggggccc ccaccggatg atccttgggc agggggccgt gcagcagttc ggtttcttcc
      481 tccagaatcg ttgcgagtgt cagcgggtcg tccggcgaag cgggagtagt catcgtgact
      541 ccaaggggaa catggtgagg gtggcgttgt ctcgacggaa agcgcccgtg tcggcgcctg
      601 tcataccacg attggaaaag gcaaaccaca tgcccagtaa ggacggtaga acagaacgcg
      661 gcccggcaac cctgtcgatg cgttccgtgc cgtctgcgga tacggaaata gacgcgaatg
      721 cgtagggcag gtatgttgtg gagattatcg aatcggatac agcgcgtatc tcaagtgata
      781 cggcaccctg gcgtcgctat ggtcaatgct tgcggtcccg ctgcatcagc ttgtcggtgt
      841 aggcgaggaa gatcgccgaa cccaggaacg tcatgtgaat aaagatcttc cacttgatgt
      901 gttcggggtt ctcgttggtc acgtccacaa aggcctttaa cagatgaatg ctggagatgc
      961 cgacgagcga agcggccagt ttgatcttga tggtgcctgg atccacatgg gtgagccact
     1021 ccgggcgatc ggggtgggtt tccagatcca gtttgctgac gaaggtcgcg tagccgccga
     1081 tgatgaccat ggtcagcagg ttggcgacca tggtgacgtc gatcagcgac agcacaccca
     1141 gcataaacag cgtttcggtt tcctcgcgga aatggacgat catgtgccaa agttccgtca
     1201 ggaacttggc ggcatagagc agttcggcga tgatgagccc gccatagagc ggcgcctgaa
     1261 tccagcggct gccgaagatc aggatttcga agagctgctc gacacgatgg gctgctgtag
     1321 acggtggaga cggggtatgg ggtgcagacg gatgactcac gccggtatgg accctccttg
     1381 ttcagggcgt atcctagtcg gcgcgtggag ttctgtcaat cgtgaggtcg cgaggcgtga
     1441 tgccctggcc gactagcccc ccgcaggcgc gtcgttcgcg acagagggtt tgaggtcttc
     1501 gggtctgaca tcgggcagcc agtgcgcaac cttcgcctgg aggtcggcgg cgatgatcgg
     1561 tttgctgatg aagtcgtcca taccggcctt caggcaggct tcgcggtcgc cggccatggc
     1621 attggcggtc atggcaatga tgggaagacg ggtacccggc tgttcctgcg aacgaatgag
     1681 cctggtggtt tcgaatccgt cgagttccgg catctggcag tccatgaaca ccaacgggta
     1741 cgggtgccgg cagatggcgg ctaatgcctc cattccggtt cccgcgaggt ccacgcgaca
     1801 gcccagcttc tccagcatct tcacggccac gcgttggttg atggggttgt catccacgac
     1861 cagcacacgc tggaccgtat gggccttgac ctcggtcagg gtgtgccgcg tgatcaattg
     1921 cgccttctcc tcacggcctt cccggccaag cgtcaggcgc atacaatcgg ccagatggcg
     1981 ctcacgaatc ggtttggtca ggtacgcggc ggcgcccgct tctttcgcga gcatgctgtc
     2041 cccgcgctgg cccgaggccg tgagcagaat gacgtgggtt ttcgcgatgg caggatcttc
     2101 cttgatgagc cggcagagtt ccagtccgct catctccggc atgtggacat cgacgatggc
     2161 gatgtcgaag ggggtgcctt cctgcgctgc ctgccgtaag aggttgagtg ctgacggacc
     2221 atcgacggca ctctcatggt gcatgttcca ggctgagaca tggtactgga gcagactccg
     2281 attcgtggca ttatcgtcga cgaggcagac gcgaaggccg ctgagattct tcacaggcag
     2341 cggaggcgtg atgggggcgg tgccttcttc cggggtagaa aacttgaccg tgaacgaaat
     2401 gcgggttccc aggcccggca tcgactgcag cccgatttcc ccgtccatca gttctaccaa
     2461 gcgtttgcag atcgaaaggc cgagccccgt gccgccgtat ttacgagcgg tcgagctgtc
     2521 ggcttgagaa aaagcttgaa acatcttggc ttgggcttcc tgcgtcaggc cgatcccggt
     2581 atcgatgacc tcgaaccgca gggtgacgga gcctgaactc tcatccgttt gcatcacctg
     2641 aatcagcact tcgcccttct cggtgaactt gatggcattg ccgaccagat tggtcagaat
     2701 ttgtctgatc cgtccgggat cgccgatcac ggttcgcggg gtctgcgcat cgatgagccc
     2761 gaccagttcc aactggcgtc cctgggcggt cggtgccagg atgtccaggg tgtcttcgat
     2821 ggtcagccgc aggtcgaagg ggatgtgttc aatggtgaat ttgccggcct cgattttgga
     2881 atagtccaga atatcgttga tgatgcggag gagcgattcg ccggaatgtt tcagcgtttc
     2941 cagatagccc ttctgctcag cggtcagctc ggtgtcgagc aagagggtcg tcatgccgat
     3001 caccccgttc atcggggtac ggatttcgtg gctcatggtc gccaggaagt cggtcttcgc
     3061 ctggccggcc cgcacggctt ggtcgcgggc ttccgagatc tcccagttct tccgttcgag
     3121 gtcctgggcc gctttcgaca gcgcgctttc ggtgcgtttt cgcatggtga tgtcgacgat
     3181 ggatgccagc gccagttttc cgttcggcgt atcgatcggg ttgagtccga gctcgacggg
     3241 aaactcctgc ccgttcttat gcaggccgaa gagttcccgg ccggctccca tggcgcggga
     3301 cgacggggcg gagaagtatc cgatgcgatg cgccggatgg tcgggacaaa accgttccgg
     3361 aatcagcatt tcgaccggct tgccgagcat ctcctctcgg ctgtagccga acatggtttc
     3421 gacctgtttg ttgacgagcg tgatgttgcc gaggggatcg atcatcaaca tgccgttcgg
     3481 cgcggattcc accacttgac ggaactgggc ctcggcctgt ttgcgcgagg agatgtcgcg
     3541 tacgataccg gtaaactttc tgacgccgtc gacgagcatc tcactcacgg ccaactcaat
     3601 gggaaatgac acgccgtcat gtcgaagtcc cagcacttca cggccgatgc cgatgacctt
     3661 tttctggccc gactgcaaat aattggtcag gtaccggtcg tgttccgtgt ggtaggggtc
     3721 gggcatgagg agtttcacgt tctgccccag cagctggtcc ggggtgtacc cgaacatgcg
     3781 agtgacagcg ggattgagcg attcgatgat gcctcgttcg ttgatgacga cgatgccgtc
     3841 caccgccgtg tcgacgatcg cgcgcaaccg ggcctcgctc tgatgcatgg cggcttccgc
     3901 ctgtttgagg tccgacacgt tgatgtgcat gaccacagcg ccgtagctcg ggctgccctg
     3961 cttcggggtg acgatgaccc ggaaccagcg tcggagtgtg ggacttgagc tctcgtattc
     4021 atagtcgaac gcacggatgg tgccgtccag gatgtgccgg attccttggg ccacgtgctg
     4081 ggcgtcgggc tgaggtcccg ccgcatcgca gatgtggaga tagttgaggc cgacgccgta
     4141 gccctcgccg gtaaagccgt tcgcggctgc gaaccgttcc caggactcat tgaccgctag
     4201 aattgtcccc ttcgcgtcga gcacggcgat attggccggc aggacgttga ggatgctggg
     4261 atcgagggca ttgtcctgcc tccgtctgat ggggaggtga tggacggcca tacagcagac
     4321 tccttcccta cccggtggtg ccggttgcag cgccggcggt tccgagccag gtgtcgagta
     4381 ccgcctgaag ctctttgggg cggaccggtt tggcgatgta atcgtccatc ccggcggcca
     4441 ggcagcgttc gcggtcgcct tgcatggcgt tggccgtcat ggcgacgatc ggggtatggg
     4501 ccgaccggcc ttccattttg cggatcagtg cggtcgcttc aaacccgtcc acttccggca
     4561 tctggcagtc cataaaaatc agcggataac gggcacgctc atgggcggcc acggcttcct
     4621 gtccgtttcc cgcgacatcg acccggtagc cgagtttctc cagcatcttg caggcgactt
     4681 tctggttcac gggattatcc tccgccagga gcaggcgagg cttcggtcct gcctgggatt
     4741 cgctcagggt gtgtcgtgtg atcaacggcg gcggttcggc gttcgacggc gtcccggccg
     4801 cggtttccgg ggacagcagg agacacaggc attcgagcaa ttgtgtctga cggagcggtt
     4861 tcgtgagata ggcatcgatg ccgatggatc gcgcagtctg gccgtctccc cgccggccga
     4921 ccgtggtgag gatgaccagg tgtacgccgg cggtggcggg gtcttgtttc agcaaggcgg
     4981 ccgtttcgaa tccgtccatg tcggagagat gcaattcgat gagggccagg tcgaagggat
     5041 tctgagcggc ggccgccgtg cgggctaact ccacggcctc cgggccgctc tgtgccaccg
     5101 tgcaagtcat tccatgcgag gtgagctcct ggcgcatggc ttgcctgacc gtgtcgcaat
     5161 ggtccaccag gagaatacgc cggccatgca gccggctcca ggaaaagggc gacggggaag
     5221 cggcgagtgt cgtctccgga aattgcacgg tgaaccagaa cgtgctgccc tgtccgggat
     5281 ggctttcaac cccgatccgg ccatgcatct ggctgacgag gcgttggcaa atagcgagcc
     5341 ccagtccggt gccaccgaat cgtctggtgg tggagctgtc ggcttgtacg aaggcgttaa
     5401 acagtcgtcc ctgcgccgct tctggaatac caatcccggt atctttgatg gtaaagcgca
     5461 aaaggcccac gccgtcttga gtctcacgcg tcacgtggag gaacacttcg cctgcggagg
     5521 taaacttgat ggcattgccg accagattca ccaggatttg ccggagacgg ccgggatcgc
     5581 ctacgacggc ggtcggcacg gcggcatcga ctaacccgat caactccagg cctttgctct
     5641 gcgcctgttc cgcgagcagc tctaccgtat cctccaccgt catccggagg tcgaacggga
     5701 gttcctcgaa atgaagtttt cccgcctcga ttttggagaa atcgagaatg tcgttgagga
     5761 gagtcagcaa gctgtcgctc gaacggcgga ccgagtcggc aaattcaagt tgttctttgg
     5821 tgagggcggt atcgagcaaa agacccgtca tgccgatgat ggcattcatc ggggtgcgga
     5881 tttcatgact catggtggcg aggaagtccg ccttggcctg ggcggctcga agggcttcat
     5941 cccgcgcttg cgcaagggca tgttctgcgg cttttcggct ggtgatgtct tttccaatcg
     6001 ccaggaatcc ggtcacgact ccgtcactgt cccggagtgc ggtgatagtc aagaggacag
     6061 tcagccggct gccgtctttt cgctggtacg tccattcccg ttcatcgaac ccgcctcgtt
     6121 tggcattttc gatcaaggcg gcaacaccat gaatcgggcg ctcatacaag tcgctgagtt
     6181 ccctggcatg gcggtcgatt tcctcgggca cgtgcagcaa ggtgagcgac tgttgcccga
     6241 tcatctcgcc ggcggaataa cccagcaact cttctgcgcc ggtgttgaag gtcgtgatcg
     6301 tccccttggt attggtggcg acgatggaca cattggttgc gctggaaagg atggtttgca
     6361 gttgcaaatt ggtcgcgagc agggcccgtt ccaggtcttt gcgagtctgg atgtcggtgg
     6421 caatgacctg gatggccggt ttcccctcga acataatcgg ggctgcggct acttcgacat
     6481 cgatgatgga gccggcgaga ccgacaaacc gctcctcggt gggcgggacc ggtcgattcg
     6541 tgtggcgtat tttcgcgatt ctctgccgta cctcggggtg cgattcggga tggatgaact
     6601 cgaggacggt cttcccgaat aactgcgacg gggcgatcgc gcccaataac ttcgcgcagg
     6661 cctgattggc aaacacgatt ctgtcttcac aattcacgaa gatagcgtgg ggtgataatt
     6721 ccacgagcgc gcgatgccgc agctcactgg cacgaatctg tgcctccatc tgtttggctg
     6781 cggtgatatc gaccgcgatg cccgcaatgc gataggtctc tccctgggcg ttcttgatcg
     6841 cgaagcttcg atcccggatc caacggacct gtccgctcgg tgtgacgatc cgatattcct
     6901 cgtcataggg gaggtgttcc tgacaggcag ctgcggtggc aacgcgtttc cggtcctccg
     6961 gatggatgtg atcgagccag aatgacggat tcacatagag gagatctctg gggagttccc
     7021 agatagcctc gaaggccggg ctgacataga ggacctcgct cttatccggc gaggtgagcc
     7081 agaagacggc atcgatatgc tcagccaatt gccggaatcg ctcttcactc tcccggaggg
     7141 tctcttccat gcgcttccgc tcggaaatgt cgcgaagcat gcccgtatac tttttctccg
     7201 ttccgatctg catctcgctg accgacacat gcacgtcgat ccgagtcccg tctttgcgca
     7261 cagccggaag gtcgcggccg ctgccgatga tggtgcgttt cccggtgacc aggtagttcg
     7321 ccaggtactc atcgtggtgt tcacgatagg gagagggcat gagtagggaa atgttctgcc
     7381 cgaccagttc gtgcgcctgg taccccagca aattcagcag ggcggtgttg gcgctttcga
     7441 tccggccgcg ttcgtcgatg acgacaatgc cttcgatcgc gctctcgaag atcgaacgca
     7501 ttctggtttc gctgtcccgc caggcaaatt cggcgcgttt gtagtcggtg atgtccagca
     7561 cggttccggc catactcacc ggtcgtccgt cggggtcgcg ctggacgact ccgcggcaat
     7621 tgacgtgccg gacttcgccg tcggggcggc gaatccggca atcgacgttg tagggcgagt
     7681 cgtatttcag cgccgcatcg accgcttgct gtacgcgctc gacgtcgtcc ggatgcagcg
     7741 cggagaggaa ggtcgcatag gtcgggaggc tctggtctgg agtatggccg aagatccggc
     7801 actgctcttc ggaccatgct tcctgaccgg tcgcaatatt ccagtgccac gaaccgagat
     7861 gggccagcgt ctgggcctca tccagcgcct gttgtttggc ctgcaagtcg cattgcgcgc
     7921 gtgcctgcgc cgcttcgagt gccttacggt cggaaatgtc gagatggatg ccggcggccc
     7981 gcaagggggc acctttagcg tcccgcttga ccacgcgtcc gctgtcgaag acccagcacc
     8041 aggcgccgga cttggttcgc aggcgatgct cgctggaata ggtgggcgtt tccccacgaa
     8101 gatgcgaaga aaccgtcgcc atcacctggt gaaggtcatc cgggtgcacc aattgttccc
     8161 aggtccggac atggggttcg agctcgtcga gacggtagcc gagcatactc gcccatcgtt
     8221 cgttaaaggt cactcgcccc gtggggattt cccagtccca ggtgcccatt tgggaacctt
     8281 gcacgatcag ctccatccgg tcggcctgct cgtccacagt ctgttcatgc acagcgagaa
     8341 ggcgttcgag gtcggcgatc ctggcctgca gatgcgcgtc gtggacgggc gttgggtcgc
     8401 cgccggcctg ctccggcttg gaagtgtggt gcgggtgaaa ggccgccgtc gtagtcgcat
     8461 agggcgccag tgcaatctgc gcgcacagcg cgaggggttt gaagagggtt gccgtgcttt
     8521 cagggataaa gtccttgctg aagagaatga tggaaaacag ttcaccgtcc ggcagcggcg
     8581 cgccgaatcc caggaccgac cggatcccgt acttgagcac gaactcttcc tgtcccggga
     8641 cgtaggggct tccttcggct tcgggaatgt gaaagacgtt gaacgattgt tcgtgctggt
     8701 cgagcaacac gctcgggccc ggctgtgtca acgaggggag cgagactccc agctgccgga
     8761 agagctggct gaacatgggc aaccgttcga cggcctccag ggtgtcgagc gggatcacgc
     8821 ggaagcggct cgaacgcgcc gggtcattcc acccggggac cgctccagcg ctggccagca
     8881 gggtgaggca ggtcagagag gggggcgggg gcgtgtctcc gagccgggcg tcagccagtg
     8941 cacgaaggtc cggtgtcagc cggctatagg gtgtcgtctt gaagaggcgg accaggctgc
     9001 aggccggctc ccggagatgg cccatggtca aggattcgta gaggtgacga acgaggcggt
     9061 cggcgacctg ctcaaggctg gtggcatctg tgccgagccg gcgcaacgcc gcgccgcagg
     9121 cggtcatgtc tcggagttgg aacgatcgca gatggtgcat gctacaggcg attccgcaaa
     9181 ggcagagcgt aggcgtcgat actctgtcgg tcggcgggag gaggaacttg agcaccgcac
     9241 catcgtgcgt caggcgatcc ggcgtcaggg aatggttttt aagtaaaagg cggcggcttg
     9301 agacctcctg ctcaggtgaa gtttcgaata cacgttggcc aggtagtttt tgactgtttt
     9361 atcgctcagc ccgagctcgg cggcgatttc cttgttcgtt tttccttgtg caataagggg
     9421 gaggaggcgt cgctcttgcg gcgacagcag atgacggccc cgttcgggct cccgatcggc
     9481 aactttgcgg aggtaggcaa acgtgtgacg ggtgaccccc gggtccaggt aggattgacc
     9541 cttggtcagg ctgcgcatgg cgtgcagcag ggtatccgcc ccggcctcct ggaggacata
     9601 cccgtgggcg cctgtcgcga cggcggagag cagggtggaa tcgttgagcg catcggccag
     9661 aaagagcacg cgcgtttccg gaagatgcgc gaggatgtct ttggccgttt cgatacccgt
     9721 gccgtcctgc acgcgcagat cgagcacgac gatgtcgggt ttgtggtgga gcaccaaggg
     9781 gagcgcctct tccttcgacg aggcctcacc gaccaccata aagtccggta cggaattcag
     9841 cagcgttcgc atgccgactc tgaccaattc ctgaggattg atgatgaaca cgcgcgccgg
     9901 agtgttctgt aattcgttca tgttcgactc tcaactagat gtggatggat gactggtgaa
     9961 ccgtttctct gtctgtgccg aggcggcggg tgcccttcga taaggggggc ccgtcggatg
    10021 atgatcggcg tccatcctgc tctaaccgga gagaagccgc gatcgaacgg ccctgcggcg
    10081 ggccggcctg tcagaggccg acccgcgcac acggccgcgc attcgatgcg cgatgtcttc
    10141 tggctcaggt tgctcatggt gcgggcctac gagactccca ccagatgggt catgctgtag
    10201 cccggcgacg cagccatcgg gttggtggtg gcgatgccgg cattcacgcg cgacagcgcg
    10261 agcacgatat tcttgccgcc gaatccaaag ctgttgatca aggcacgatc gagatgcgcc
    10321 gcccgcccct gccccgagac atagtccaga tcgcatgtcg gatccggcgt cgtcaggttc
    10381 agcgtgggtg ggatgaactg gtgctccatg gccttcgcgg tgccgatgac ttggaaggcg
    10441 ccgctggcgg cgaaggcgtg gccgagtgcc gctttgaacg aactgacggg aacccgatac
    10501 gcgtggtccc ccaggacctg tttgaccgtg ttcgtttcga gcgcatctaa ctccatggtg
    10561 ccgagcccgc aggcgttcac gtagtccact tcctccggga accaatgggc cgtcgagagg
    10621 gccagcctgg ccgcccgttg ctgctcgtgt cccgtgtggt ccgccttcac catggaatgg
    10681 gcatcacagg taaagcccca tccttccagc tcggcataca tcttggcgcc gcgggcgcgc
    10741 gcgtgctcat agtcttccaa gatgaggcag ccggcgcctt cgcccaacac catgccgcat
    10801 cgggttttgt cgaaaggacg gggaaggttg cgaatggccc cgcgttcgtc cggcgccagc
    10861 gcgcgcccgg cgttcatggc ggcgtagacg ctcgggtgga gcggcgcttc ggtcccgccg
    10921 gccacgacga gcgacgattg gcccgacttg atccattcgt aggcgcggcc gatggcattg
    10981 gccgtcgagg agcagccgat cgagtacgtc tcgcaggggc cgtgcagccc gagcacgatg
    11041 gccacttccg acgaaatcgc attcgggaag gtcatcccca tggtgaatgg attgacgcgc
    11101 tcataggcgt tgagtttggc cgcatcgtgg aactcgaacg cttccttcat gccgccgatg
    11161 gaggtgccga tgctgatgcc gatggacccg cgatcttcct gttccagctc gacgccggaa
    11221 tcgcgggctg ccatcatggc ggaggcgacg gcgaactggg tggcgcgccc catacgacgc
    11281 gcctgcttgc gatgcaggaa atcttcgggg tcgaagtccc gcacctccgc cgcgaggcag
    11341 gcttggaacg gtgaggtatc gaacgagctg accggtttga cggcgctctc acccttgctc
    11401 aatagatgcc agaacagatc gacggtacaa cccaacgggg aaatcactcc cattccggta
    11461 atgacgactc gacgagaggt ctgtggtgtc atggtgccct cctgtgctcc ggggagcgtg
    11521 aaaatgtgtg tcgtcagtgg ctgtgccaca catgcatgtg ctcgcgatgc gcgtccggta
    11581 acgtggaatc gggataaaac gtcgcataga actcttcgtc caggtgatgg gtttgacagg
    11641 ccacgatgga cttgcaggca tcggcgtgcg ccgggaaccg tccgtaggtg ccgagcacgt
    11701 agtcggcaaa cgcgatggtc gcgtcgatgg cgcgctcgga atgctgcggc acctgctgcg
    11761 ctgctttggg attggtccaa ggctgctcgc tgggctttcc gtaggtgccg ttcggcccga
    11821 acttgctggc gaccaggctg gtcacggcat ccttcatcga cggcacgaac ggcggggtgg
    11881 ggccttccca cacctgcgga atgccgaccg gattggcgcg caggggcttg cccggcggga
    11941 cgacgaagtg gaacccgagg ccggggtaga tgtgcggttc cataccgagg aggtggcggc
    12001 cgctccccac gctctggatg ccgccgccca gtcccagggc ctgctgcatc agcgagagat
    12061 gttcgcagat gatcccttgc tcttgaacgg cggtttcgca aatgccggcg tcgagctcgc
    12121 gtaggctgaa cattttgcgc gaggcgagat tgtcgtgcag atgtccaccc gcgctcttgc
    12181 ggaaggcatc gaggccgcag gcggcgttgc cgttgtccgt atcgacgatg aagtatcccg
    12241 tgtcttcgct gaacatcgtc agcaagagat tcaggtacag cagcgcgatg ttggtgacag
    12301 gcatgaacaa ggtggtgccg ggccgatttg tataccactg attgaacggc agcatgaacg
    12361 gggcttcgcg cgggatctcc agccgggacg attggagttg caccacctcc agatcccagg
    12421 gatggtcggg accggccgcg tcggcgacaa aaaacacacc gtcatcgttg gtgtaaaaga
    12481 gttgcgtgct ctgcgcggag caggggctgg gaaccgtgcg gctgttgaag ttcatcagcg
    12541 cgtgaccctg gccggcactc gcgcccgggc ggccgacaaa ctgcatgtcg cccaggttgc
    12601 gcccggatcg gccgatggcc gcatagatca gatatcgttc ttcttcccgg ctcagcggga
    12661 tcggttcatg ctgactcgtg tattgaagcg ggccgccggg cagggtcatg ccgcggccga
    12721 aacggcgcgt cttgcgttgt tgcaggaggt cgagcagagt acatgtggcg tccattgctg
    12781 caggttgtgc ggcaagctgc ttcatagggc ctctccttcc cggcatccta gggcaggatg
    12841 gccccggccg ggtgtgtgaa tgagggtggt gctggtacgt cacgcgcggc atgacgttgt
    12901 gtgggacgac tgtacgagag gcgtgatctg gcgtcaccta cccatcgggg taggcgatcg
    12961 ccctacccgg cgtcggtcaa ccggtgacgg acggggcgga gcgcgagtcg gcatgcatcg
    13021 agcgatttag tcgggtgtgt tggccccgga gggcggacgg tcgtcactgg gcggtgcgga
    13081 tggaacgatg cgatgttgtt cgccggtttg ccaataggca atggcgctcc atcgattttc
    13141 gaccccgatt ttctgaaaga cgttcttgag atgaaatttg acggtgttca aactgacttt
    13201 cagaatgacg gagatttccc aattggtctt gcctgccgcc acccatcgga gaatttcttc
    13261 ttcccgtttg ctcagtaggg tcggcggcgg ggcagatcgc tccggattcc acgcggagtg
    13321 gcatcgcata taactcaggt gaaaatgcgg ggtcagaatg tcgagcagga gccggagttt
    13381 gtcggcttgt ttctcatcga aattactgaa ggcgaagtac gaacaagacc cattcgctcc
    13441 acgcactccg gccgacaggc aggtcttgat gccgaagtcg agtttcagcg agataatctc
    13501 cttgggctcg ttggcggtcg gttcatcggc gctggaggtc acggtctgac ccgaggtgat
    13561 gaggcggttg acggctggat cgaccgctaa tccttggccc atatacagat gacagaattc
    13621 ccgcgggtag gtggaatgcc ccagtgaagg atcgatccca cgcaggagat cgacggcgcc
    13681 gcatccggag aattcgtagg gaatcacttt ctgcacggcc gagacgagtt gccacaccct
    13741 gtcgctgcga tcgacggagc gtgcatcatg catgatttcg ccgagccgtt gaaattcctt
    13801 ggcggaaaat gctggctgac tcatgccgat tcttcccgat cagcggggcc gggtgcgagt
    13861 gggagcaccc gaggccatga agattcagaa gcgcggacgt ggttcatagt gagacagctg
    13921 tgccctgatg aaacaggctg attgtactgt ccgcttgagc ccggtatcta gggcaacctg
    13981 cacctagtat agagtgctta aggtttgaac cgcaacgggg aagaggaggg tcttgaaaat
    14041 tgacatggcg ccggcgggag gcccgcaggt cgtcggacta gcggtgaatc ttcagggtcg
    14101 gcccgagcgt cagccacttc gtggggccga gggcggccat gtgcggcaga tgggctacgt
    14161 accagaacac cacatcctgt tcctggacgc cctggttgtc ctcgtcataa ccgagctggc
    14221 cccgggctcc gaagacccat gagagatctt cgtccgcatt ggcccggcga atggccacgt
    14281 cgaggtcggc gaaggaatcg cgttctccgt cgtctttcag gggcgcgtag ccgtctccgg
    14341 gtaacaccca taccccatgt cctgtgagtt ggtcgcgaat aaaccaggcc cggtgagtgg
    14401 ccgtatgttt cacgtcattg cgttcattgg tatatttttt ccatcccggc ccccagccgc
    14461 tgtccgggcc cccatcgtcg cgcacaaata cctgatccat tccgttgccg ttgatgtcga
    14521 aatcgaagcg ccaataggga tggtggacgt gatcggtatt gcaggacaac ccccggctct
    14581 ggacgaccgg ccgcaattca ccgtcttcgg acagatgcca ggattggtaa atgtgatact
    14641 ccccgattcg ggcatagatg cccagttcca gccacttcat gcctttcacc gtcgagcttt
    14701 cgacgcagac cttctggtcg ccgcagttca caatcgggac gagatcctgc cagcgcagtc
    14761 gatcttgaaa ggggccgcag cgtccgctgt cggcgcggga gccgaaccag gtaaaggggt
    14821 tccaccagat gcgttccttc acgtatttca cgcgcaggac cggcatgctg gctttggcca
    14881 gcacctgttc tccgccgtag gtgacgttgc gaagggccaa gccggaattg tcccggactt
    14941 cgtagtcgaa actccacgca ccccactcga catgctccga tgctgtgtcg gccgagccgg
    15001 gtagcggcag cgcgagggtg cagagcagta acaaggcact caccgcgagg cgtctcatcc
    15061 cgtatccttt caccgcctaa cggggcggtt catttcctgc atccagcaca cggtcttccg
    15121 tgagatcgac ttgtgcccag tatttggggt cgccgcccag accaggggag aaggtgatcc
    15181 aaatcactcg atgtccatac ccggggtcgt ttctgaagaa accgcgatcc ggctgcatca
    15241 acagcccatg tccttgcatc ccttgcactt tgtcggcgag acggcggtcg gcgcgagcca
    15301 gctcgatgcc ccgttggata tcccgggggc cttcgggggg tagataccct tccgctcgcg
    15361 aggcttccag aatggtcgaa tctttcatgt gcacgtggac ggcgacgtta tggctgtagc
    15421 tgaagtaggt gagtctggtg aatcgcgcgc ttgtccgaca gcagccgaac gacaccttcc
    15481 ccctcggtgg tactcgatcc gcgtcaatga atccccatcg ttcaccgagg agggccgcga
    15541 ctcgaggatc cgtttccgcg acttttcgca gtcccgcaat gtcggcagct gagagcaccg
    15601 cctcccgatt gggtgagcgg gagacaccgg catcgagtgc gcttccggcc atcttgggct
    15661 tgatatgctc gggggattca tcggatgaga attccaggac atgtctggtc gggagctgtg
    15721 cgggcagtgg cttctttggc acggcggttt ccggatcgtc cggaccgaag gggccgcgtg
    15781 aggcggcatc gagactgcag cctgtggcga gaaggagcag catcaacgcg gcggatgtcc
    15841 gcatgaccgg tcgagttccc atacctctca caccaatgcc agtttgccgg ccacgccaag
    15901 tgccaaacgc gaaatctgca cgcccgcttt aatgacggcc gcgcgttttt ttcgcgacgc
    15961 ggcatccagc acggccttct tcatcaagtc tttatagccg ctgatttcgt cggcgccgaa
    16021 ggggacgatt tgggccactg tctcgttatt gaaggtgttg gccagttgca ggtacagatt
    16081 ggagatctcc gcggcataga tgcctcgtcg cagcgggtcg ttcttgtagg tctcaaactt
    16141 cttccgcagg gcggcgacgg cctccagcag ggctgcttgc cggaccttcg tatcgtcctg
    16201 gcggccgaca ctgaactcca ccgcccccaa ctgatcgacc agcacttgcg cttcctcgcg
    16261 ggcgttgccc acgcgatcgg ccagttgttc gatttcattg gtggcgcttt cggcggtttc
    16321 catcagctgc ttgatcagac tgtcattgtc ttggaggaaa gtgaccaccc cctcgatact
    16381 gcggttcata gagcctccgt tactgtccgt cgtccttctt caatcgatcg aggagttcca
    16441 ccaggtcggc ggtgaacagc tgagcacgat ccagtccgcc gatgcgcaga attcgcgcgc
    16501 cgacgacttc ttccagggca tcgttcacgg ttcgcgagtg atccaggatc tccatgatct
    16561 ggccggaccg gttgggggcc tggtcaagga cggccgtgac gaactcagga tcgacggatg
    16621 cgaaggccgt ttgcttggca aatcgatgca cctgggcatg cagcgtcgcc gtccgtttcc
    16681 atgagtcgtt gagggcattg gccgtatcgc ggaggccgca aaagtcctcc tcaaattgat
    16741 tccagaccgc cttctcgagt ccctgggcat cggccatgta gatgaggctg gtgaggtagg
    16801 ctgccgcgta ggcggagtct cccttcttcg tggtcaggtt ccggaaggag gccagggcga
    16861 accggtgttt ggtttcctga atctggagtg atttgaagct ctcgtgcagc gaactgcgcg
    16921 cctgttcgag gaacttgatg cgggcctcgg tttccttctg gaagaggtcg atgttggtct
    16981 gcgcggcgtc gatcttggtg ccttccagtt gaatgaggtc gcgcaccgaa cggctgtgga
    17041 tcgagtggca accggtggcc ataaggaaca gtatgacgac ggacagcagc ctcacgcctc
    17101 gcgtgaccat ggagggctcc ttcttccggc cggctgtgcg gcggatcatc ggcgcaccgt
    17161 gcggagcgcg tcggcaagcg actgggtgtc gttccggctg agagtgacgt cgatgtccag
    17221 gtactgtttg atgatgccgt tgatccggcg tagcgcttcg acattattcc ggtactggtc
    17281 gaacgccgtg agatcgagag cctgcggggc cttggaatcg gaggccacgc gctgctcgat
    17341 ccgcgagatc agttcgggaa tttgctggat gagatgactc atgtccccgg agccggcgag
    17401 aaaaacaggc tgcccgttcg cgcccttgcg ttcggggaga gatttgaggc gcatggcatt
    17461 gatcacagcc aggacatcgg gacccagcac ctcggcatcg tcgtcggcga gggtggcgtc
    17521 cgggatgtcg gtcagccggg gattggtcgt ggcccattgc aaggccagat ccagcttgag
    17581 acgagtctgg acatagcgga accactgcac atggcggctg ttgatattgc cgaccagctc
    17641 ccgatactgc tgcatgagct gttcattttc ggtatagccc tgatccttgg ccgtcagcgc
    17701 ctgcttgacc tccggcgggg tggtgcaggc ggcgagcgag gcgaagatca gtatgtgaac
    17761 gatgcgcaac atcctcatcg ggatgcctcc ccattatcgt cttaagtcgg atgcccggag
    17821 agagacgcaa actacgtacc tggcaagcag caattgctcc gctgtcgaac gagtcgcaaa
    17881 aacccaatat tcgaagagaa agtgtgcctg ttccactgtg aggcatcgcc ggtgcggcgg
    17941 atcgagcgta cctattcaga tacgggtgta tcgaacccaa tacagtttat gcgaagtctc
    18001 tctcggtatt gcgttagctc gcattactca tgacggtgcg tcgc
//
LOCUS       DAOQRM010000040        17963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2237_length_17963_cov_15.771443, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000040 DAOQRM010000000
VERSION     DAOQRM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17963
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2237_length_17963_cov_15.771443"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PKK81_05205"
     CDS             <1..624
                     /locus_tag="PKK81_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32299.1"
                     /translation="AHDLRNPLGVIRSSAQVVQEGPQEEPIRQEMARYIIDEVDKLSL
                     RLHDFLRYARQKPPDFKVEPAEAVVRAALRQWEAQGGHERIRVEERFEHALPSIQVDP
                     EQVKEALMNLLINAREAMPEGGTLTLTTRAGQDSEVEIEVADTGNGIAPEHLSRIFEP
                     FFTTKAYGTGLGLTNVKRLVEDNGGTLVVNSNAGAGTSFTLRFRAAG"
     gene            complement(1095..1940)
                     /locus_tag="PKK81_05210"
     CDS             complement(1095..1940)
                     /locus_tag="PKK81_05210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV32300.1"
                     /translation="MTSLMALSMTVAGCAEVQRAAEDVARQSGNPRLAGAIHGAGNVV
                     GSLFPIGYEEESSIGQAIALQVVARYGGVVDQPELVRYVNLVGRAVANTSDRPDIPYH
                     VAILDHESINAFAAPAGYIFVTRGLLRQIKNEAELAAVLGHEIAHVSEKHILDVIQRS
                     KRLAGVTEAGLSYATSNPAAFKGVIDGAVKKLLDEGLDQEKETEADTVGELFAARVGY
                     DAEAYVGLLTRLRDLKGDDRALFKTHPNFSARIEAVQKTIRTKRLVSNGLVLQERFLR
                     MTKRV"
     gene            complement(1951..2463)
                     /locus_tag="PKK81_05215"
     CDS             complement(1951..2463)
                     /locus_tag="PKK81_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV32301.1"
                     /translation="MIPPRSFWIPLIALWIGLFVWGTEAWAETVYVQAKTAQLRAGKT
                     SLDAVVGNVKFGDALEVVGRDGSWMEVKTSAGARGWIFANKTSTSKPSGGNDTLARLG
                     QSMRGGDASATTASAGARGLDKASEGYADRAGVSARDREVVDRMTAYQIPDQDVEEFM
                     REGGLGEYAK"
     gene            complement(2490..4628)
                     /locus_tag="PKK81_05220"
     CDS             complement(2490..4628)
                     /locus_tag="PKK81_05220"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV32302.1"
                     /translation="MTSTPFQRKILSAVAVGLTVFALVAGLSTSRWFEVAELKALDHL
                     VRRYADPAKANSNLVLLAIDESSLEAFGRWPWPRDRFGYVVRYLKQAGARAVVFDVMF
                     FEADENAEEFDQSFADDLKAAGNVFLPMLFQAEPAAIPPEAQPRATVTVESSDARQAP
                     DTHAGVKLPIPVLAQQARGLGVINLSADADGPTRRVPLLGQVNGNVVPHLSVAVAQYL
                     LGADRLSMRDGRLQIGAHHVPLETDGSLLIDWHGSLEQTYHAKKYSIGRVLQAFAQQE
                     KGERPSLDPSLFKDKVVFIAGTAAGLYDLRVTPFSAATPGVLIHMAALDNLLHGQGLQ
                     AAPRWFSLTTLLLLCLASAGTFMLFRSYPVKFGVTIGLAVAYYGLVVHAFAGHERWLE
                     LVFPEAALALTFGTAATVEYVTEGKQRRLMRAAFDKYMSSEVVEEIMRNPEAIKLGGE
                     KKEITIFFSDIAGFTTISEKMSPEDLVTLLNRYLSAMTTIIKTTHRGNVNKYLGDGIM
                     ALFGAPLNDPKHASLACYAALDCQVELARLREVWKRERLPEIGARIGLNSGPCIVGNM
                     GSEERMEYTVTGDSVNLASRLEGASKYYDTLILIGQRTAELAKNDVEVREIDLLRVKG
                     KKEPVVVFELLARKGQLDDKKRHVIDVYLEGLAAYKMRNFSTACARFSEAVALDPSDG
                     PSRVYLERSTNYRQMPPPGEWDGVYEMTSK"
     gene            complement(4908..6521)
                     /locus_tag="PKK81_05225"
     CDS             complement(4908..6521)
                     /locus_tag="PKK81_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNV32303.1"
                     /translation="MKIYLVLLVLLFAPVESGRANERPPLPVEKPAGSGVIVHRDGYV
                     LTAHHVVANAKRITIVTSGEFRAPAVLVSVDAEHDLALLKVETVGLSEALLGYAGAVK
                     LDQEVIAVGFQFGLREITITRGHVAAVRTKGVQRVFQVDAAVNPGNSGGPVFNRKGEV
                     VGILTTKFTHPSGIVPEGMAFAVPISYATPLLANIPDFDFSAIGRGRRGSPKGKGNGD
                     LVTELTRTSVRIETVRMSEGPATVIHPAPMPPSSDGRHSAQPSVTRVPVPPAPVHEPV
                     PLAGDESIDRVNAQLLASQQEELKRFVDQGLTPPGEMVLIPAGEFLMGMEDGLPDARP
                     IHRLYVSAYWIDRHGVTNGQYRACVEGGACLIPKVRDAFDDTQRAQFPVTNVTWSQAR
                     AYCQWVGKRLPTEAEWEKAARGIDGRRYPWGNSDEVIQKSRVKLTDGNSANGVDPLGL
                     PSVSASPYGVFGMIGMVSEWVKDWYAEDFYRTSPARDPQGPLRGTFRVLRGGSWMERP
                     LELRASYRGWDEMTYWGPTLGFRCATDVP"
     gene            complement(6518..7297)
                     /locus_tag="PKK81_05230"
     CDS             complement(6518..7297)
                     /locus_tag="PKK81_05230"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV32304.1"
                     /translation="MRRLPWMLACLVAAGWPSALPFAWGLESSDRSTIPFHTILELEQ
                     TARLLAILLDSGRAVVNDNQTLLDDPTKAHKGFTPEAFEGQLRDMFRGRAGINLDELA
                     GGTLSPRSRHLLQELVTVSKQVIADAQAELNRQGAGFKGFIPAVFGARVSTRFTAVTG
                     VRLKQTALDPRNPANRPDSYERVALEAFADSAYPREKVISEMAANSSSLRLMFPLYAT
                     RQCLDCHGGPKGGLDRTGYPREGLTLGQNAGAISVLLPVGK"
     gene            complement(7440..7949)
                     /locus_tag="PKK81_05235"
     CDS             complement(7440..7949)
                     /locus_tag="PKK81_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32305.1"
                     /translation="MRWKACLVVAAIGVFFPVASGWADSTDSPLMGVRSAVSGQIVKI
                     ESGLLFVKTPYGLQFRTISPNKADRVGLHNARLGDEVWLLIDSGNVLLDAARPGGEHF
                     ANHKVVAGRIHYADPFWGEIQISTPEGFERFEVDALAGSKLSVFQEGMPVTIELDSDN
                     VMIDIQSGR"
     gene            complement(7990..8385)
                     /locus_tag="PKK81_05240"
     CDS             complement(7990..8385)
                     /locus_tag="PKK81_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32306.1"
                     /translation="MTIQNRGYGICALVFGLCVGLALLAGSVSAQQSWVDEINNSLNF
                     YKTSYPGSNWEPYSQRLQTVKEAVDRADTRTVKSEMGKWFKMLRAREQGIHDVAADEL
                     FNFALMVTPILEYGITVPPAPGGGTEPGY"
     gene            complement(8422..8751)
                     /locus_tag="PKK81_05245"
     CDS             complement(8422..8751)
                     /locus_tag="PKK81_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32307.1"
                     /translation="METGSRIKSVCVIVGGLGLGLFIASGGQAQVIHPDQMIGVPSPQ
                     TEIGSGESPTSSEPSVNDMEKGFDRHMMVPESGASGSMDTNKLKTPDATDAENARESD
                     RQSSVSF"
     gene            complement(8907..9551)
                     /locus_tag="PKK81_05250"
     CDS             complement(8907..9551)
                     /locus_tag="PKK81_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV32308.1"
                     /translation="MPITIVIAEDQRLFRQSLRLLLEHERDLVVVGEAMDGRQAFDLA
                     VAHKPGIVLMDVDMPRLDGITATRLIRSCVPDSKVLMLSVHDDDARIVAAVQAGACGY
                     ILKDADHQEFLRIIRATFEGDVVRSPFMPDGFAKKAMALVNKQGESEGGGLSLLTERE
                     HEILACAAAGRSNKEIADQLCVSLDTVKTHLHHIYQKLRVSGRVEAVLTYLQSR"
     gene            complement(9533..11839)
                     /locus_tag="PKK81_05255"
     CDS             complement(9533..11839)
                     /locus_tag="PKK81_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32309.1"
                     /translation="MRQRVSLQSLITLSVLSVGILSGTVGLVYAYWHAKQSLHTTVGI
                     TFQEIARQSADKAALLLAKELEWMQRLSALPEIRASVEGVSSAKQPTLQFEQWREEQH
                     RYFHSLVIVRWDGQLVGGRQSESARTFYAQQPWWPVVFSAQRAWAGPLTVDDNGRGFW
                     EVAVPIGGQGQPVRGALKAVIGTDRILSSIVGSRIGRTGHMMLLAEDGVVLACSMLAP
                     NLHATLQTQSMQAPADAPAARWLPLEQDTHGGNQSIIGLARVVLPAQIEQARGWFILM
                     QQDPSETFEPLLALMGKLGAFWVGAVVFIVWLRWRLARRIVRPLGDLIQRVNVFGGAW
                     PSEPLPSQAFSRPSGIVEIDALAASFDDLAQRLEQASRVKAQYVRRLEQVNLDLATSE
                     EHYRLLWNHSVDTKILIDLFGSILDINRRGEVTLGRPASTLARRPFTDLVAESERPRL
                     LEQLESVIRSGADLSGGAMHVPIPTGDLTMEVDWVPVKKAGRIESIMIQLRDLTEQKA
                     LERQVVRSERLASLSQFASMFAHDIRNPLAGIKKTLEWLGGCPEMGQDPPRRCLEDLR
                     FTTDLLLGMINDMLDVYQENYSGLPLSTSPVSPSLLMHEVLRLFRSEAEAQEVRFNVQ
                     VPSEAVWIMGDGRRLQRVLINLIHNALKYSPARGAIMISVQVNRGQVSQRETVAEPDE
                     CSTVTIQIQDEGPGIAPEDLPHLFEMFFRKKDGQDYRIGRGLGLHFCRLVVDAHGGQI
                     TAANRPEGGAVFTVALPVRQEQVCPSPS"
     gene            complement(12030..14462)
                     /locus_tag="PKK81_05260"
     CDS             complement(12030..14462)
                     /locus_tag="PKK81_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNV32310.1"
                     /translation="MQSETLQSESCNVLEWAKLLDHLASYAQSAIGAERCRSLVLEIN
                     LSQAQMRQQETSDMLRLRSGVDPFPVLRFPNLAEWLGRVAKGACLEAHELRDIALVLG
                     LIDDVQRYLLRHQADAPAVGAMVVQLGPVEEYRRLTIALNAAIDPDGSMRESASPELH
                     RLMQRANDLKRQMRHRLEQILHSRRYEEVLQEHYFAQREGRYVVPIKAEMQGRIPGIV
                     HDVSSSGATVFLEPRELVDLNNSIKVVDLDVEREVRRILRELSAMVAPHQPALAGSVA
                     LLGELDAISAKAGLSQRLQAVPPRLNEQGRILLNRARHPFLVLTKEQVVPNDLVFDET
                     VRVLIISGPNTGGKTVTLKLLGLFALMVRCGLHLPCAPESEMAIFPSVYADIGDAQDL
                     ARDLSSFSAHITQMVQLLKEVSEGTDVGTSPSPIHPGRALVLLDEPVTSTDPAEGAAL
                     ASALLCRLAAAGVKVVATTHYSLLKGLAQETPGFANASVEFNIDTLSPTYRLIQGQPG
                     GSAAIEIAGRLGMDESLLQDARARLQGDNRLLETLLNDLQDKQRRMSEDLAAATAAKQ
                     SAEQAARESQELLAFLQESERDERQGLKKKLSQEFQRARAAVQATLDDLKRDQKLLKA
                     KETKVRLIELEQAVSREVRAAQEPIPVDQLSVGDAVEVVGLGMTGTLLESPQGKKRVR
                     LKVGEGEILANVGSLVGLARPPQPARSEPRKTTTVTSRRTSQPLAPEDIQETLDVRGQ
                     AADDALDIVVAGLDRAIFGGSPFVRIIHGHGTGRLRAVLRDYLKASPYVVTFRPGDRA
                     EGGDGVTIVELR"
     gene            14693..15700
                     /gene="gap"
                     /locus_tag="PKK81_05265"
     CDS             14693..15700
                     /gene="gap"
                     /locus_tag="PKK81_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNV32311.1"
                     /translation="MTTRIGINGFGRIGRNVLRASLGDPSLEFVAINDLTDAKTLAYL
                     LKYDSVHGTLDASVEAKDDQLIIDGKAIKVLAVRDPKELPWKALGVEIVVESTGHFTD
                     REGAGKHLSAGAKTVIISAPAKDPDATVVLGVNEQVFDAKAHHIVSNASCTTNCLAPV
                     AKVLLENFGIKHGVMTTIHSYTNDQQLLDLPHKDLRRARAAGMSMIPTSTGAAKALHL
                     VIPQLKGKLDGLAIRVPTPNVSLVDLTVETEKDCDVAAVNAAFKKAAEGPMKNVLAYS
                     DAPIVSIDLKDDPHSAIVDAPLTAVIDKRLVKVTAWYDNEWGYSCRVRDLIKYIVAKG
                     A"
     gene            15796..16995
                     /locus_tag="PKK81_05270"
     CDS             15796..16995
                     /locus_tag="PKK81_05270"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNV32312.1"
                     /translation="MNIRKRIIEDVQLRGKRVIIRADYNVPLDDSLQITDDTRIRSTL
                     PTINRAVDEGAKVILCSHLGRPKGRFDPKFSLAPVAKRLQRLLGKEVIFAPDCIGAAV
                     EGLVAKMQPGDVMLLENLRFHPEEEKNDDGFSKALASLADVYINDAFGAAHRAHASTV
                     GITKYIPEAAAGYLLKKEIEYLEGAVENPVRPFVAILGGAKVSGKIGVIENLGKKVDK
                     VIIGGGMAFTFLKAMGLEIGQSLVENDMLDFAKGVQDHAFSSGVKFYLPVDCVVAASR
                     EPGAETKIVPVQEIPKGWYGLDIGPASVKLFSEAVQDAKTILWNGPMGMFEVDAFARG
                     TLAMAHSVANAYALTIVGGGETALAIHRAGESESISFISTGGGAALELLEGKTLPGLA
                     ALPNRAA"
     gene            17035..17814
                     /gene="tpiA"
                     /locus_tag="PKK81_05275"
     CDS             17035..17814
                     /gene="tpiA"
                     /locus_tag="PKK81_05275"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNV32313.1"
                     /translation="MRTRFIVGNWKMNKTASDAGAFVHRLSQELPHHAGIQIGLTPPF
                     TALHAVREALGPASAVLLGAQNLYWEDKGAFTGEVSGAMLKDIGCQFVLVGHSERRQY
                     FGDQDSWTNKKVLAALRHGLQPILCVGETLQDRDSGQTDLVIQRQLRAGLAGIEAHTL
                     PTLTIAYEPVWAIGTGRAASPEQAVPVHRLIRRTIGELSGTDIGNHVRILYGGSVTPH
                     NIADFLASEEIDGALVGGACLDPVSFATLVKVAIGSKPTTV"
     gene            17824..>17963
                     /gene="secG"
                     /locus_tag="PKK81_05280"
     CDS             17824..>17963
                     /gene="secG"
                     /locus_tag="PKK81_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNV32314.1"
                     /translation="MYTLLIIIHVIVCLLMIGAILLQSGKGAEIGAAFGGSSQTVFGS
                     RGP"
BASE COUNT         3756 a         5805 c         5004 g         3398 t
ORIGIN      
        1 gcccatgatc tgcggaaccc gctcggcgtc atccggagtt ccgcacaagt cgtgcaggag
       61 gggccgcagg aagaacccat ccgccaggaa atggctcgct atatcatcga tgaagtcgac
      121 aaactttcac tgcgactcca cgactttctc cggtatgcca gacagaagcc gcctgacttc
      181 aaggtagaac ctgcggaagc cgtcgtccgc gctgcgctga gacagtggga agcacagggt
      241 ggccatgaac gcatccgggt cgaagagcga tttgagcatg ccctgccctc gattcaggtc
      301 gatccggagc aggtaaagga ggccctgatg aacctcctca tcaatgcgcg tgaagcgatg
      361 ccggaaggag gaaccctgac cctcacgacc agagccggac aggacagcga ggtggaaatt
      421 gaggtggccg ataccggaaa cggcatcgct ccggaacacc tttcacgaat cttcgagccc
      481 ttcttcacga ccaaagccta tggaaccggt ctcggcctca ccaacgtcaa acggctcgtt
      541 gaagacaacg gcggaaccct ggtcgtgaac agcaacgcag gagcgggaac atccttcacc
      601 ctgcgctttc gtgcggcggg ctagaaacgc acaagggata tcaacggcgg aaaatctacc
      661 attgttttct agccggccgt tcaacatggc cgacagcggg gccgcagcaa gcgacgggac
      721 gaggcgtacc cttgcggtac gtgaagcccc tgagcgacgc gagaacaaag ccagcggtaa
      781 tctcagtagt cacggaggag gctcaaacag gctgtccagc aaggccgcag cgagtgagga
      841 gccgagtcgt acccttgcgg tacggcgagg gccggaacga cgcgagaacg acgttggcgg
      901 cctgtttcag cctcctgcta gcccggatta ttcatgaatg ccgtcgcgag gtgttcgaga
      961 cggaagcccg ccgaggctgc tcgcacgtaa ggccgacgca ggcgtactgg cagtccgtcg
     1021 aggaggccga acgagaacag cctcgccggg cgacaggatc ggacgccgga gcagggattc
     1081 atgaatagtc cgggctagac gcgtttggtc atgcggagga accgttcctg caacaccaag
     1141 ccgttggata caagacgctt ggtgcgaatg gttttctgca cggcctcgat ccgcgcggaa
     1201 aagttgggat gggtcttaaa cagcgcacgg tcatcgccct tgagatcgcg caaccgcgtc
     1261 agtaggccca cataggcctc cgcatcgtat ccgacccgcg cggcgaacaa ttcaccgacc
     1321 gtatcggcct ctgtttcttt ctcctgatcc agaccttcat cgagcagctt cttgacggcg
     1381 ccgtcgatca cgcccttgaa cgcagccgga ttactcgtcg cataggagag gccggcttcg
     1441 gtcactccgg ccaggcgctt gctgcgttga atcacatcga ggatatgctt ttcgctcaca
     1501 tgcgcaattt catgccccag gacggcggcg agctccgcct cattcttgat ttgcctgagc
     1561 aggccccggg tcacaaaaat atatccggcg ggcgcggcaa acgcgttgat cgactcgtga
     1621 tccagaatgg cgacatggta tggaatatcg ggccggtcgg acgtgttggc caccgccctc
     1681 cctaccaggt ttacatagcg aactaattcg ggctgatcca caacaccgcc gtaccgtgcc
     1741 acgacttgca gggctatcgc ctgcccgatc gacgattctt cctcgtagcc gatgggaaac
     1801 agactgccga cgacattgcc tgcgccatgg atcgcaccgg ccaatcgggg attgccggac
     1861 tgtcgggcga catcctcggc agcccgctgc acctctgcac aaccagccac agtcatcgag
     1921 agcgccatca ggctcgtcat ccagatcctg ttatttcgca tattcgccca ggcctccttc
     1981 ccgcatgaac tcttcgacgt cctggtcggg gatttgatag gccgtcatgc gatccactac
     2041 ctcccgatcc cgcgcggaca cgccggcccg gtcggcataa ccctcggacg ccttgtccag
     2101 ccctctggcg cccgccgatg cggtggtcgc ggacgcgtct ccgccccgca tgctctggcc
     2161 aagccgagcc agggtgtcat tgcctccgga cggtttcgat gtcgatgtct tgttggcgaa
     2221 gatccatcca cgcgcgccgg ctgaagtctt gacctccatc cagcttccat cgcgacccac
     2281 cacctccagc gcgtcgccga acttcacgtt cccgaccacg gcatccagcg aggtttttcc
     2341 tgcgcgaagt tgcgccgtct tggcttgcac gtagacggtt tcagcccagg cttccgtccc
     2401 ccagacgaac agaccgatcc acagggcgat gagcggaatc caaaacgatc gtggtggtat
     2461 cacagcttcc tccccctcgg cactgccact tattttgagg tcatttcata gacgccgtcc
     2521 cactctccag gcgggggcat ctgtcgataa ttcgtggatc gttccaggta gacccgcgaa
     2581 ggcccatccg acggatccag ggcaacggct tctgaaaacc tggcacaagc cgttgagaaa
     2641 ttccgcatct tataggccgc cagcccttcc aaatacacgt cgatcacgtg ccgcttcttg
     2701 tcatccagct gccccttccg ggccaacagt tcgaacacga cgaccggctc ttttttccct
     2761 ttcactcgta gcagatcgat ctcccgcact tcaacgtcgt tcttcgccag ttcggccgtc
     2821 cgctggccga tcagaatcag cgtgtcgtaa tacttgctgg cgccctccag acgcgaagcc
     2881 aggttcacac tatccccggt caccgtatat tccattcgct cctcggaacc catgttgccc
     2941 acgatgcatg ggcctgaatt gaggccgatc ctggcgccga tctccggaag ccgctcccgc
     3001 ttccagacct cccgcaagcg ggccaactcg acctgacagt ccagcgccgc gtaacaggcc
     3061 agcgacgcat gcttcgggtc gttcagcggc gcgccaaaga gcgccatgat cccgtcaccg
     3121 agatacttgt tcacattgcc acgatgggtg gtcttgatga tggtcgtcat cgccgacaag
     3181 tagcgattga gcaatgtcac gaggtcttcg ggagacatct tctccgagat cgtcgtaaag
     3241 cctgcgatgt cggaaaaaaa gatcgtgatt tcttttttct ccccgccgag cttgatggcc
     3301 tcgggattcc gcatgatctc ctcgaccacc tcggacgaca tgtacttgtc gaaggccgcc
     3361 cgcatgagac ggcgctgctt gccttcggtc acatattcga cggtcgcggc cgtgccgaac
     3421 gtcaaggcca gcgccgcttc cggaaacacc agttccagcc accgctcgtg cccggcaaac
     3481 gcatgtacga ctaatccata gtaggccacc gccaatccaa tcgtgacgcc gaacttgacc
     3541 gggtaggaac ggaacaacat gaacgtgccg gcagaagcca gacagagcag caggagggtc
     3601 gtcagcgaga accacctcgg agctgcttgc aagccctgcc cgtgcaggag attgtccaac
     3661 gccgccatat gaatgagcac accgggcgtg gcggcagaga acggcgtgac acgaagatcg
     3721 tagagccccg ccgccgtacc ggcgataaac accaccttgt ccttgaacaa cgacggatcc
     3781 agcgacggcc gttcgccctt ctcctgctgc gcgaaggcct gcaacacccg tccgatcgaa
     3841 tacttcttcg catggtaggt ctgctccagg gatccatgcc agtcaatcaa caggctgccg
     3901 tcggtctcca atggaacgtg gtgggcgccg atctgcagac gcccgtcacg catcgagagc
     3961 cggtcggcgc cgagcaggta ttgcgccacc gctaccgaga gatgcggcac gacattgccg
     4021 ttgacctgcc ccagcagcgg aacccgcctc gtcggtccat cggcgtcggc agacaggttg
     4081 atgacaccca gcccgcgcgc ctgttgtgcc agcaccggaa tcggcagctt cactccggca
     4141 tgagtatccg gcgcttgtct cgcatccgac gactccaccg tgaccgtggc gcgaggctgc
     4201 gcctcaggcg ggatcgcagc gggctcagcc tggaacagca tcggcaggaa cacgttgcct
     4261 gccgccttca ggtcatcggc aaacgactgg tcgaattctt ccgcattctc gtccgcctcg
     4321 aagaacatca catcgaacac gacggctctg gcgccggctt gcttgagata ccgcacgaca
     4381 tagccgaatc gatcgcgagg ccagggccag cgcccgaatg cctcgaggct cgattcatcg
     4441 atggcgagta acacaagatt cgaattggct ttggcaggat ccgcatagcg tcggaccaaa
     4501 tgatccagcg ctttgagttc tgcgacctcg aaccagcggg aggtgctgag cccggccacg
     4561 agggcgaaca ccgtcaggcc caccgcgacc gccgaaagaa tttttctttg aaagggagtg
     4621 gaggtcatga aggcgattgc acgatcaaag acacgctgac catcgggcta tcgtacgtga
     4681 gaatgaaccg gagggaaagg gggctggagg gggccttttt cagcacccgg ctggcccgga
     4741 tgattcctga gggccgtcgc gaggcgttcg agaccgaagc ccgccggggc ttctcgcaag
     4801 tgaggccgac gcaggcgtac ttggagtccg tcgaggaggc cgaacgagaa aagccccgcc
     4861 gggcgagagg atcggacggc ggagcaggca gtcaggaatc ttccgggcta ggggacgtcg
     4921 gtcgcgcaac gaaatcctaa cgtcggcccc cagtacgtca tctcgtccca gccccggtag
     4981 ctggcgcgca actcgagagg ccgctccatc cagctgccgc ctcgcaagac acgaaacgtc
     5041 ccgcgcaacg gcccctgcgg atcccgggcc ggcgacgtgc ggtaaaaatc ctccgcatac
     5101 cagtccttca cccattccga caccatcccg atcatgccga acacgccgta gggtgacgcg
     5161 gagactgagg gcaggcccaa cggatcgacg ccattggcag aattcccgtc cgtcagcttg
     5221 accctgcttt tctgaatcac ctcgtcgctg ttaccccagg gatatcggcg gccgtcgatt
     5281 ccccgcgccg ccttctccca ttccgcctcc gtcggcaatc gtttaccgac ccactgacag
     5341 taggcccggg cttgagacca ggtgacattg gtcaccggga attgggctcg ctgagtatca
     5401 tcgaaggcat cgcgcacttt cggaatcaga caggcgcctc cctccacaca ggcccggtac
     5461 tgcccgttcg tcactccatg ccggtcgatc caataggcac tcacatacaa ccgatggatc
     5521 ggacgggcat cgggcaaacc atcctccatg cccatgagga actcgccggc gggaatcaac
     5581 accatctccc cgggaggtgt cagtccttga tcgacgaatc gcttgagctc ttcctgctgg
     5641 cttgccagca gttgagcatt cacgcgatca atagattcgt ctccggccag gggaaccggc
     5701 tcatggacag gagcgggcgg cactgggaca cgggtgacgg acggctgggc actgtgccgg
     5761 ccatcggaag acggaggcat cggtgcgggg tgaatgaccg tggccggccc ttcggacatg
     5821 cgcacggttt cgatccgcac ggacgtccgc gtgagttccg tgaccagatc accgttgccc
     5881 tttcccttgg gcgaccccct tctccccctg ccgatggcgg agaaatcgaa atccgggatg
     5941 ttggctaaca ggggcgtggc ataactgatc ggcacggcaa aggccatccc ttccggcacg
     6001 atcccggacg gatgggtaaa cttcgtagtg aggattccca cgacttcgcc cttccggtta
     6061 aacaccgggc ctccgctgtt gccgggattc accgcggcat cgacctgaaa gacccgttgc
     6121 acgcccttgg tcctgacggc agccacatgg ccacgagtga tggtgatttc gcgtaacccg
     6181 aactgaaacc cgacggcaat gacctcctgg tccagcttca cagcgccggc atacccgagc
     6241 aacgcctcgg agagccccac cgtctccacc ttgagcaagg cgagatcgtg ttccgcatcc
     6301 acgctcacca gcacggcggg cgcccgaaat tccccggacg tcacgatcgt gatccgcttc
     6361 gcattggcaa cgacatggtg ggccgtgagg acatacccat cacgatgaac aatgacgccg
     6421 ctaccggccg gcttttctac cggcaatggc ggacgctcgt tcgcccgtcc actttcgaca
     6481 ggcgcgaaca acaggacaag caggaccagg tagatcttca ttttccgacc ggcaacagca
     6541 cgctaatggc accggcgttt tgccccaacg tgaggccttc ccgcggatag cccgtgcgat
     6601 cgagtccccc cttcggaccg ccatggcaat cgaggcactg tctcgtcgca tagagcggaa
     6661 acatcaggcg tagcgacgaa ctgttggcag ccatttcact gatgactttt tcccgggggt
     6721 aggcggagtc cgcaaaagcc tccaatgcga cccgttcgta cgagtcaggc cgattggcag
     6781 gattccgggg atccaaggca gtctgtttga gtcgtacgcc cgtcacagcg gtgaagcgcg
     6841 tggacacgcg cgctccgaac acggccggaa taaacccctt gaacccggcc ccctgccgat
     6901 tgagttccgc ttgagcatcc gcgattacct gcttgctcac cgtcaccagc tcctgaagca
     6961 gatggcggct ccgcggggat agcgtaccgc cggccaactc atccaggttg atgccggctc
     7021 ggccacggaa catatcgcgt agttgtcctt caaaagcctc cggcgtgaag cctttgtgtg
     7081 ccttggtcgg gtcatctagt aaagtttgat tgtcattcac gactgcgcgg ccggagtcga
     7141 gcaggatggc cagcagacga gccgtctgct ccagttcgag aatggtgtga aagggaatag
     7201 tcgaccggtc ggaggactca agcccccagg caaacggcaa ggccgagggc caccctgctg
     7261 ctaccaggca cgccagcatc cacggcagac gtcgcatgag ccgcctctgg tgcattcgcg
     7321 ttgcggactg cgagaaccgc gcgctcctgc ccctgattac ctggcaggag cgcgcgtcag
     7381 taaaaatcgt acgagcctct cacgatgaag tcaaggcgac gacacgcacg acagtcacat
     7441 taccgaccag actggatgtc gatcatcacg ttgtcggaat ccagctcgat cgtcacaggc
     7501 atcccctcct gaaataccga cagcttgctt cccgccagcg cgtcgacttc aaaccgttcg
     7561 aatccctcag gagtcgagat ctgaatctcc ccccagaagg ggtccgcata gtgaatccgg
     7621 ccggccacga ccttgtgatt ggcaaagtgt tcaccgcccg gcctggccgc gtccagcaac
     7681 acgttccctg aatcgatcag taaccagact tcatcgccga gccgagcatt gtgcagcccc
     7741 acccgatcgg ccttgttcgg actgattgtc cggaattgca acccataggg agttttgaca
     7801 aacaggagcc cactctcgat tttgacgatc tgcccactca ccgccgaacg cacacccatc
     7861 agcgggctgt cggtcgaatc agcccatcct gatgccaccg ggaagaaaac ccctatcgca
     7921 gcgacgacta ggcacgcctt ccacctcata acggcctcct ttcgcaccaa ctcacatcca
     7981 ggcatcgact caatagcccg gctcggtgcc tccgccgggc gccggtggaa ccgtgatgcc
     8041 gtattccagg atcggcgtga ccatcaacgc aaagttgaac aactcgtcgg cagcgacatc
     8101 gtgaatgccc tgctcacggg ccctgagcat cttgaaccac ttgcccattt ccgacttgac
     8161 cgtccgggta tctgcacgat ccaccgcctc tttcacggtc tgcaaccgtt ggctgtaggg
     8221 ttcccaattc gaaccgggat agctggtctt gtagaagttg agggagttat tgatttcatc
     8281 gacccaggac tgctgggcag acacagaccc tgccagcaat gccaagccga cgcacaaccc
     8341 gaaaaccaac gcacagattc catatcctct gttctgaata gtcatgatgc gcctcctctc
     8401 aatgacacga tcccacccgg atcagaagga gaccgacgat tggcggtccg actcgcgcgc
     8461 attctccgca tcggtggcgt ccggcgtctt cagcttattc gtgtccatgc ttccggatgc
     8521 gccgctctcc ggaaccatca tgtgacgatc aaaccccttc tccatgtcgt ttaccgacgg
     8581 ctcggaggat gtcggactct ccccggaacc gatctccgtt tgcggcgagg gaactccgat
     8641 catctggtcg ggatggatca cttgcgcctg tcccccggac gcaatgaaca gccccaggcc
     8701 caacccaccg acaatgacgc acacagactt gatacgtgat cccgtttcca tgatgtatcc
     8761 ctcctccatg aaggatgacc gcattgcaca ggcactctgc ggcagcactt agaacagtac
     8821 tggaagcggg acacggggcc tattgcctgg agggtggatt ttccctccac cctttgagtg
     8881 aggacttgga gggaatgaac ggggatctac cggctctgca gataggtgag tacggcttca
     8941 acccggccgc tcacacggag cttctgataa atatggtgca gatgggtctt gacggtatcc
     9001 agggacacgc acaattgatc ggcgatttct ttgttgctcc gtccggcagc cgcgcaggcg
     9061 agaatttcat gctcgcgttc cgtaaggagc gacaacccgc cgccctccga ttcgccctgt
     9121 ttgttcacca gggccatcgc cttcttggcg aagccgtcgg gcatgaaggg agatcgcacc
     9181 acgtcgccct cgaacgtggc gcgaatgatc cgcagaaact cctggtggtc ggcgtctttg
     9241 aggatgtacc cgcaggctcc cgcttgcacg gcggccacga tccgggcatc gtcgtcatgt
     9301 accgacagca tcaacacctt gctgtccggc acgcagctgc ggatcaatcg cgtggcggtg
     9361 atgccgtcca atctgggcat gtccacatcc atgaggacga tgcccggctt gtgtgccacc
     9421 gcgagatcaa acgcctgccg gccatccatc gcttccccga ccacaacgag atctcgctca
     9481 tgttccagca gcaggcgcaa gctctgccgg aacaaccgtt gatcttcggc aatcacgatg
     9541 gtgatgggca tacctgctcc tgcctgaccg gcaacgcgac cgtaaagacc gcgccgcctt
     9601 cgggacgatt cgccgccgtg atctgcccgc catgggcgtc caccaccaac cggcagaagt
     9661 gcagccccag gccgcgtccg atccggtaat cctgcccgtc tttttttctg aaaaacatct
     9721 cgaagagatg cggcaggtcc tccggcgcaa ttccaggccc ctcatcctgg atctgaatcg
     9781 tcacggtcga acattcatcc ggctcagcga cagtttctct ttgtgagacc tgtcccctgt
     9841 tcacctgcac gctaatcatg atggcgcccc gtgccggcga atacttcagc gcgttgtgga
     9901 tcaaattgat caacacccgt tgcaagcgtc ggccgtcccc catgatccac accgcctccg
     9961 agggaacctg cacattgaaa cgcacctctt gcgcctcggc ctcggagcga aacagtcgta
    10021 acacttcgtg catcagcaga cttggcgaca ccggcgacgt actcaacggc agtcccgaat
    10081 agttctcctg atagacatcc agcatgtcgt tgatcatgcc cagcaggaga tcagtggtga
    10141 agcgcaggtc ctccagacag cgacgcggcg gatcctgccc catctccgga cagcctccaa
    10201 gccactccag cgtcttcttg atcccggcca gcggatttct gatgtcgtgg gcaaacatcg
    10261 aggcaaactg gcttaacgac gcgagccgct cggaccgaac gacttgccgc tcaagcgcct
    10321 tctgctcggt gaggtcgcgg agttggatca tgatcgattc aatgcggccc gctttcttga
    10381 ccggcaccca atcaacctcc atcgtcaggt caccggtggg tatcgggacg tgcatggccc
    10441 cacccgacag gtccgccccg ctccggatca ctgactccaa ctgctccagc agacgaggcc
    10501 gctccgactc agcgacgagg tccgtgaacg ggcgccgggc gagggtcgag gccggccgcc
    10561 cgagtgtgac ctcccctcgc cgattgatgt ccaggatgga accgaacaga tcgatcagaa
    10621 ttttggtatc gaccgaatga ttccacagga gccggtaatg ctcctccgac gtggcgagat
    10681 caagattcac ctgctccaac cggcggacgt actgcgcctt cactcgggat gcctgttcca
    10741 atcgttgcgc gagatcgtcg aaactcgcgg ccagcgcatc gatctcgaca atcccgctgg
    10801 gccgggaaaa ggcctgggac ggcagcggtt ccgacggcca tgcgccgccg aagacattga
    10861 cccgctgaat caaatcgccg aggggacgca cgatgcgccg ggccagccgc cagcgcagcc
    10921 acacgatgaa cacgacggct ccgacccaga acgcccccag tttccccatg agggccaaca
    10981 acggctcgaa cgtctccgac ggatcctgct gcatcaggat gaaccagcca cgggcttgct
    11041 cgatctgcgc cggcaggacg acccgggcca gaccgatgat gctctggttc ccgccatgcg
    11101 tatcctgctc caacggaagc catcgagcgg cgggagcgtc cgctggtgcc tgcatagatt
    11161 gtgtctggag ggtcgcgtga agattggggg caagcatgga acaggcgagc actaccccgt
    11221 cctcggccag cagcatcatg tgtcctgtgc ggccgatgcg acttcccaca atcgaggaca
    11281 ggatacggtc cgtcccgatc acggccttca gggcccctcg caccggctgc ccctgtccgc
    11341 cgatcggcac tgcaacttcc cagaaacccc gcccgttgtc gtcaacagtc agtggaccgg
    11401 cccaggcgcg ttgagccgag aacacaaccg gccaccatgg ttgctgcgca taaaaagtcc
    11461 gtgcggattc gctctgccgg cctccgacca attggccatc ccatcggaca atcaccagcg
    11521 agtgaaaata acgatgctgc tcctctcgcc actgctcgaa ctgcagcgtc ggctgcttcg
    11581 cagaggacac cccttccacc gaggctcgaa tctcaggaag ggcactgagc cgttgcatcc
    11641 attcgagttc cttggcaagc aacagggccg ctttgtccgc gctctggcgg gctatttcct
    11701 ggaaggtaat gccgactgtg gtgtgaagtg actgcttggc atgccagtag gcatacacga
    11761 ggccgactgt gccggaaagg atgcctaccg atagcacaga gagggtaatc aaactctgga
    11821 gactgacccg ctgacgcatg gcggcaggcc attggaggga gcgactcgac cggctccttg
    11881 gaccaggcag cgagttctgc cgggagggaa tgccagccgg accgcacatc ggttacgtca
    11941 tgtcacactc cactgctttc gggcgtacac tcgccacccc ttcagcatac aaaatttcgg
    12001 ttgccgaaca taggcctcac aatgcacggt catcgcaact cgacaatagt gacaccatcg
    12061 cctccctctg cgcgatcacc ggggcgaaag gtcacgacgt agggagacgc cttcagatag
    12121 tcacgtagta cagccctcag tcggccggtc ccgtgcccat gaatgatgcg cacgaacgga
    12181 ctcccgccga agatggcccg atcgagcccg gccaccacga tatccagtgc gtcgtccgcg
    12241 gcctggcctc gtacatccaa cgtctcctga atatcctcgg gggccagggg ctgactggtt
    12301 cgcctggacg tcaccgtcgt cgtcttacga ggctcgcttc gagcgggctg agggggtcgt
    12361 gcgaggccga ccagacttcc cacgttcgct agaatttcgc cttccccgac tttcaggcgc
    12421 acacgttttt tcccctgcgg gctttcgagc aaggttcccg tcatgcctaa cccgaccacc
    12481 tccaccgcat ctcccaccga cagctgatcc acgggaatcg gctcctgcgc cgctcgcact
    12541 tcacggctga ctgcttgctc cagttcgata agccggactt tggtttcctt ggccttgagc
    12601 aacttttggt cgcgcttcag gtcgtcgagc gtggcttgga ccgcagcccg ggcacgctga
    12661 aattcctggg acagcttttt cttcagtccc tgtcgctcgt cccgctcaga ctcctgcaga
    12721 aaggccagca gttcctgaga ctcgcgcgcc gcctgctcgg cactctgctt ggcagccgtt
    12781 gcggcagcga ggtcctcgct catacgccgc tgtttgtcct gcaaatcatt cagcagggtc
    12841 tccaggagcc gattatcgcc ctgcagccgg gcgcgggcgt cctgcagcaa cgactcatcc
    12901 atcccgagac gcccggcaat ttcgatggcc gccgaccctc ccggctgccc ctggatcagc
    12961 cgataggtcg gggagagggt gtcgatgttg aactccacac tcgcattggc gaagcccggg
    13021 gtctcttgcg ccaacccttt caacaagctg tagtgcgtgg tggcgaccac cttcacgccc
    13081 gctgccgcca accggcacag cagagcgctc gccaatgccg cgccttcagc cggatccgtg
    13141 gacgtgaccg gctcatcgag caataccaat gcccgtcctg ggtgtatcgg cgagggcgaa
    13201 gtgccgacgt cggtcccttc ggacacctcc ttcagcaatt gcaccatctg cgtaatatgg
    13261 gccgaaaaac tggacagatc ccgcgccaga tcctgcgcat cgccgatatc cgcatagacc
    13321 gaagggaaaa tcgccatctc cgactcgggc gcacagggaa gatgcagacc gcagcgcacc
    13381 atcaatgcga acaatcccag cagtttcagc gtgacggttt ttccgcccgt gttgggtccg
    13441 gaaatgatca ggactcgcac ggtttcatcg aacaccaggt cattcggcac cacctgttcc
    13501 ttggtcagga ccaggaacgg gtgccgtgcg cgattgagca ggatacgccc ctgctcattg
    13561 agacgcggcg gaacggcctg cagccgctgg ctcagcccgg ccttcgcgga aatggcatca
    13621 agctcgccca gcaacgccac actgccggca agcgccggct gatgaggcgc caccatcgcc
    13681 gacagttccc gcagaattct ccgcacctca cgttcaacat ccaaatccac gactttgatg
    13741 gagttattga gatccaccag ctcgcgaggc tccagaaaca cagtggctcc gctcgacgag
    13801 acgtcatgca caatgccggg gatccggccc tgcatctcgg ccttgatcgg gaccacgtag
    13861 cgcccttccc gttgcgcaaa atagtgctcc tgcagcacct cctcataccg gcgtgagtgc
    13921 agaatctgct cgaggcgatg ccgcatctgc cgcttgagat cattggcgcg ctgcatcaac
    13981 ctgtgcagtt cggggctggc tgattccctc atcgatccgt cgggatcgat ggcggcattt
    14041 aaggcaatgg tcagccggcg atactcctcg accggtccaa gctgcaccac catcgcaccc
    14101 acggccgggg catcggcctg atgacgcagg agataccgct gcacgtcatc gatcaatccc
    14161 aaaaccaacg caatgtccct caactcgtgc gcctcaagac aggcaccctt ggcgacccgg
    14221 cctaaccact ccgccaaatt gggaaatcgg aggaccggaa agggatcgac cccggacctc
    14281 aatcgaagca tgtccgacgt ttcctgctgg cgcatttgag cttgcgacag gttgatttcc
    14341 agaaccagcg atcgacaccg ctctgccccg attgcagact gggcgtagga ggccaggtga
    14401 tccagcagct tggcccattc cagcacgttg cacgattcgg actgtaaggt ttcagattgc
    14461 atcgcacccc gactgccggt tgatggttac gaaaaaacag actctacccg accgcccggg
    14521 cgcaggcaag gcaagcgcaa accatttgag cgacaattac gcaacaatca ccgttacccg
    14581 atcaccacag tcgattccct ctttttgtat gcttgacaca cagaaagtcg gtcagtacta
    14641 tcgccccaga ctcaatcaat tccacccgtt taattcttga ggatttagcc ccatgaccac
    14701 acgcatcggc atcaatggat tcggacgcat cggccgcaac gttcttcgtg cttccctggg
    14761 agatcccagc ctggaatttg tcgccatcaa tgacctcacc gatgcgaaaa cattggccta
    14821 tttgctcaag tacgactcgg tccatggcac gctcgacgcg tccgttgagg ccaaagacga
    14881 ccagctcatt attgacggga aggccatcaa ggttttagcc gtcagggatc cgaaggagct
    14941 tccctggaag gccctcggcg ttgagatcgt cgtggaatct acggggcact ttaccgaccg
    15001 tgaaggagcg ggaaaacatc tctcggcagg ggccaaaacc gtgatcattt cggcgcccgc
    15061 aaaggacccc gatgccacag tggtcctcgg cgtgaacgag caggtgttcg atgccaaggc
    15121 acaccatatc gtatccaacg catcctgcac caccaactgc ctggccccgg ttgccaaagt
    15181 cttgctggaa aacttcggca tcaaacacgg cgtcatgacg acgattcatt cctacacgaa
    15241 cgaccagcaa ctgctcgatc ttccccacaa ggacctccgc cgtgcgcggg cggccggcat
    15301 gtcgatgatt ccgaccagca ccggtgcggc caaggcgctt cacctggtga tcccgcaact
    15361 gaaggggaaa ttggatggac tggccatccg ggttcccacc cccaatgttt ccctggtcga
    15421 cctcacggtc gaaaccgaaa aagattgcga cgtggcagca gtgaacgccg ctttcaagaa
    15481 agccgccgaa ggtccgatga aaaatgtgct ggcttattcg gatgcgccga tcgtctcgat
    15541 cgatctcaag gacgatccac actccgcgat tgtcgatgct ccgttgaccg cagtgatcga
    15601 caaacgactc gtcaaggtca ccgcctggta cgacaacgag tggggctatt cctgccgcgt
    15661 gcgggacctg atcaaataca tcgtcgccaa gggggcctga gccgactctt acacaacggg
    15721 cttttctgac gtgcagcgca aatcggcgtg aatatcgcta ggctgcacac accagacgag
    15781 gagtgaaggt ctgtcatgaa tattcgcaaa cgaatcatcg aggatgtcca gcttcgtggg
    15841 aaacgcgtga tcattcgcgc cgattacaac gttccgctcg acgattcatt gcagatcacc
    15901 gacgacacca gaattcgatc cacgcttccc accatcaatc gtgccgttga tgagggagca
    15961 aaggtcatcc tctgctccca cctcggccgg ccaaagggca gattcgatcc gaaattcagt
    16021 ctggcccccg tcgcaaaacg tctccagcgc ctgctcggca aagaagtgat ttttgccccg
    16081 gattgcatcg gggcggcagt cgaaggtctc gtcgccaaga tgcagcccgg cgacgtcatg
    16141 ctcctggaaa atctccgttt ccatccggaa gaagagaaga atgacgacgg attctccaag
    16201 gccctggcat ccctcgccga tgtgtacatc aatgacgcct tcggggccgc tcaccgggcc
    16261 catgcctcca cagtcggcat cacaaaatat attcctgaag ccgccgccgg ttatctcctc
    16321 aagaaagaaa ttgaatatct cgaaggcgcc gtagaaaatc cggtgcgacc gttcgtcgcg
    16381 attctcggcg gagctaaagt gtccggcaaa atcggcgtga tcgagaacct cgggaaaaaa
    16441 gtcgacaaag tcatcatcgg cggcggcatg gcttttacct tcctcaaagc catggggctg
    16501 gagatcggcc aatcgctggt tgaaaacgac atgctcgact tcgccaaggg cgtgcaagac
    16561 catgcctttt caagcggcgt caaattctat ctcccggtgg actgtgttgt cgcggccagc
    16621 cgtgaaccgg gggctgaaac aaagatcgtt ccggttcagg aaattcccaa aggctggtac
    16681 ggactggata tcggccccgc ctcggtcaaa ctattctcag aagcagttca ggatgccaag
    16741 accattctct ggaacggtcc catgggcatg ttcgaagtcg atgcctttgc cagagggacc
    16801 ctcgcgatgg cccattccgt cgccaatgcc tacgccttga ccatcgtcgg gggaggcgaa
    16861 accgccctcg caatccatcg ggccggtgaa tccgaaagta tctcgttcat ttcaaccggc
    16921 ggtggagcgg cgctggaatt actcgagggg aaaacccttc cgggactcgc agcccttccc
    16981 aaccgtgcag cataacgaca acctccaccg tttcctcccc gcaatagagg ctccgtgaga
    17041 actcgcttca tcgtcggcaa ctggaaaatg aataagaccg cctcggacgc cggggcattc
    17101 gttcatcgtc tatcgcagga attgccccac catgccggaa tccagatcgg cctgacaccg
    17161 ccatttacgg cgctgcacgc cgtacgcgag gcgctgggcc cggcctcggc cgttctcctc
    17221 ggcgcgcaaa atctctattg ggaagataaa ggagcattca ccggcgaagt gtccggggcg
    17281 atgctgaagg acatcggttg ccagtttgtc ctcgtcggcc attcggagcg gcgacagtat
    17341 ttcggcgacc aggacagctg gacgaataaa aaagttctag ccgcccttcg gcatggcctt
    17401 caaccgattc tgtgcgtggg agaaacactc caggatcgtg attcgggaca gaccgacctc
    17461 gtcattcaac gacagcttcg cgccggactt gccgggatcg aggcacacac acttccaacc
    17521 ctgacgatcg cctatgagcc agtgtgggcc attggaactg ggcgggctgc gtcaccggag
    17581 caggcggtcc cggtccatcg cctcatccgc cgaaccatcg gcgaactgag cggaacggac
    17641 ataggaaacc acgtccgcat tctatatgga gggagcgtca caccgcacaa tatcgccgac
    17701 tttctagcat ccgaagaaat cgacggcgca cttgtcggcg gggcttgttt agacccggtc
    17761 tcttttgcta cactcgtcaa agtcgcaatc gggtccaaac ccaccacggt ctaaagactc
    17821 aacatgtata cactgctcat catcattcat gttatcgtct gcctgctcat gattggagca
    17881 attctgctgc aatccggtaa gggcgctgaa atcggtgcgg cattcggtgg ctccagccaa
    17941 accgtattcg gcagccgagg gcc
//
LOCUS       DAOQRM010000041        17664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2303_length_17664_cov_17.078255, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000041 DAOQRM010000000
VERSION     DAOQRM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17664
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2303_length_17664_cov_17.078255"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..778)
                     /locus_tag="PKK81_05285"
     CDS             complement(20..778)
                     /locus_tag="PKK81_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32315.1"
                     /translation="MSDFLTRLVQRQAGTLATIQPRTRSMFAPAMDRPEPPTPDLSVI
                     DGVASSGEQRQPAEAPRHSIAPMPPLAAFLHAQRRIEAPVDEPSVVLPETGARGTEPS
                     NESPVVKVTAVPSAAPGEGPPVSLRNQIVESDRHAARVFSRDNARPEERRSEATAAQE
                     RAITPPPRLVEAQQATPMSARVTPPSLVSTGQQGRPGDAAGLTSPEPPVHVTIGRIEV
                     TALPPPSPPGRKSAARPPSMSLADYLARRQGRGA"
     gene            complement(775..1515)
                     /locus_tag="PKK81_05290"
     CDS             complement(775..1515)
                     /locus_tag="PKK81_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32316.1"
                     /translation="MAHRSQSGLSAAAVIDIWEQGAGRHPLDQALLLLRYACPDEPFE
                     TLCEWTVGERDRRLLESRRNTFGDRIDGYAECPACCNGLEFELSCEGLLGSASMTDAT
                     WHKVEQAGRSWDVRGPNSRDLAAAAAAPDLDRARRVILSRCLRNGPDTVDESDWTDEL
                     QAALAARLSELDPLAEILIDLRCAACGHAWQSVFDIAGFFWNEIHVRGRRLLQEIDLL
                     ARTYGWTEGEILGMTDHRRRLYVGMALS"
     gene            complement(1518..>2051)
                     /locus_tag="PKK81_05295"
     CDS             complement(1518..>2051)
                     /locus_tag="PKK81_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNV32317.1"
                     /translation="EEENMAQFTVNTHRFDPYKNFKFRVKWDGRYVAGVSKVGSLKRS
                     TEVVEHREGGDPSTSRKSPGRTKYEAITLERGVTHDTAFEQWANKVWNFGSGLGAEVS
                     LKDFRKDIIIELYNEAGQLAIAYKVFRCWVSEYQAQSDCDANANAVAIQTLKLENEGW
                     ERDYEVTEPSEPTFTEP"
     assembly_gap    2052..2151
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2152..3756)
                     /locus_tag="PKK81_05300"
     CDS             complement(2152..3756)
                     /locus_tag="PKK81_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HNV32318.1"
                     /translation="MPVQPTYPGVYIEELPSGVRTITGVATSITGFLGKAVRGPVDQP
                     VTITSFSDYERIFGGLHRDMTLSYAVRDFFLNGGGQAVIVRLYKATAGSAAKATYEVP
                     NLTLQAASEGAWGMQVRARVEKKAVTDPNLIAVATRLGVAPADLFDLIVRDGSTGITE
                     TFLNLTTKESARRADRVLLAESNLVRVTTTMPSNSSPTAHAGTSADADVWTSTTKSTP
                     AKNAAPADIAVESAVLDAAAYKGSQTGKTGLYQLEKIDLFNLLCILPDARGGDVPDDV
                     YQEALSYCVKRRAMLIVDPKAAWATVGAAQSGAPGMNLNGDMARNAAIYFPRIKQADS
                     QLGGQVETFVPCGVIAGVMARTDSQRGVWKAPAGLDASLSGVAGLQLDMTDAENGLLN
                     PLGINCLRTFPVHGRVVWGSRTMRGADAAADEYKYVPVRRLALFLEESLYRGTQWVVF
                     EPNDEPLWAQIRLNLGAFMHNLFRQGAFQGTAPRDAYFVKCDKETTTQNDINLGIVNI
                     VVGFAPLKPAEFVIIKLQQMAGQIAT"
     gene            complement(3782..4060)
                     /locus_tag="PKK81_05305"
     CDS             complement(3782..4060)
                     /locus_tag="PKK81_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32319.1"
                     /translation="MEEHDAEFRRSILDYLVHHPGAKDTQEGVLNWWITAPCLDEGAE
                     RLAIEALDKLVAQGWVLKRDTSNQPIYSLNHAHLKEIRTFLTQDGNET"
     gene            complement(4287..5519)
                     /locus_tag="PKK81_05310"
     CDS             complement(4287..5519)
                     /locus_tag="PKK81_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV32320.1"
                     /translation="MAGRWPDVPILGLFAAEHGRRPLLLNGVPAELNDFVLCPVDGDE
                     LALRIGRVLVAGRSSTPHGIGRSQRVRIDSLVGESSIFQTQVEKIPLFADVDATVLLQ
                     GETGTGKEVFARAIHYSSARKDHAFIPINCAALPDQLFENELFGHVKGAYTSAASEQG
                     GLVLEAEGGTLFLDEVDALNPYAQAKLLRFVQYREYRPLGCSRTKLAHVRIIAASNHD
                     LRQAVTERQFREDLFHRLNVLSLVVPPLRERVEDIPLLADRFLQRFRRADGQGPRRLS
                     EDAIQKLLAYAWPGNVRELESTLQRAVVMCGSATIRAQDIECAGEAPKSPCLDGSLRA
                     AKTSATMDVERAYLVKTLVTFRGNVTHAAKAAGKERRSFQRLLRKYGIDREAFQNDPT
                     DSSRDCPSPFQTPFDGRA"
     gene            6295..7089
                     /locus_tag="PKK81_05315"
     CDS             6295..7089
                     /locus_tag="PKK81_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HNV32321.1"
                     /translation="MLLLFCGGCSEVRDSHELLHSLLWMRTSAEYHVLSVMTYQRARD
                     ALDRALIDTRSTASIEQTGDFQHLPPAVILDLDETVLDNSPFEARLIAQRTTFNQPMW
                     EQWVQEASAQAVPGVLEFIAEARKKGVALFFVSNRGAHQESSTRRNLEKLGIHLPIDP
                     DALLLEGEPPFRWPPNKSSRRRYLAERYRILLLIGDDLGDFVDGARDKPENRIALARQ
                     HDHRWGRSWFLLPNPMYGTWETSLTPPGLSDTDRLTFKRELIRNAP"
     gene            7496..8671
                     /locus_tag="PKK81_05320"
     CDS             7496..8671
                     /locus_tag="PKK81_05320"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNV32322.1"
                     /translation="MTRADKRTTSCSPNLDFLRASAVLIVYFFHLFITTNTRLPDYLG
                     QFGVLLFFVHTSLVLMFSLERIEATEGSFFLTFYLRRLFRIYPLSIFCVSVIVLFQLP
                     RAPWWPWSNPDLSTILANLFLYTEFMYKPVVTSVLWSLPYEVAMYIVLPFLYLAGKAY
                     GVRGIFVLWCVAVVGAIAQPYVSSRLGVAQYAPCFIAGVASYFLGFGMQRFRLPFIGW
                     PLTLLIAGAILGYGNHSEHEITAQWALCVLIGVTAPFFAELQLPAMRKACALIARYSY
                     GIYLTHLHAQWTAFVLLKDSPSAVRYSVLVVLSVGLPVALYHLIEAPMVRMGARLAQR
                     LPRTSAQSSKPTPAQRPFPIPTSAAPHSTQHAAARPRAAHAPDHRHSQTTPARVSAS"
     gene            9371..12730
                     /locus_tag="PKK81_05325"
     CDS             9371..12730
                     /locus_tag="PKK81_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNV32323.1"
                     /translation="MANLTATKRWEVEQTLTATPKARRGEAAPAPLTLDVEGAPDDAY
                     IVMTRYASGAIRFHMPTEPARRGIRRGGKRIYRFSIPVPAAPIAEAGGRRGFISAAIK
                     AVVLKVAGKIADFALSKLALLWETATWKLKKQREGWLSITADSLGGESGLPAADLQTL
                     SASERNLLFIHGTFSSTKAAFRSLAQTPGSNGRTFFEELQAVYGNRIYGFDHFTLSRT
                     PQENVQMLLEALPARPTTFDVITHSRGGLVLRHVVERRDLFGSLADRFVLGRAVLVAS
                     PNEGTPFASPDHVTQYTTWLSNVLELFPDNPFTTGVEFVSEALSWIARRLVGGLPGLA
                     SMDNKGDIIQALQATPNPPNETYSALVANYEPDAGVLQRIIDAGADLFFPTANDLVVP
                     TEGGWRVDVGSGGTVTIPGTRVGCFGLGGNLTQQKAVNHVNFFDDPGTVDFLVRALRG
                     EAQPAAPIDIEHDLPSGKRRGAVRTEARPTIAAGPALQQPATISPTVAATPASKAVPP
                     IVSLTQSPEPDDVFQLALIDPDPRDAVATLIATFRNARVMETVHIGTGSTAGTARDKQ
                     DHRSAQWKNIDAMQQHIHGYIDGDPRYPQLPGEKDLQQFGGALFNALFPDQVRRLYDA
                     ARSEQVTQRLNLIFTSDNHWLARQAWEFIYDPDRRTFLALEEVNFTRNVLTSIPAERI
                     PARTGPMRILVVVAQPLGLGALSVEEETDVIRSGFRRLLDAKLADVELLLDATPELLH
                     RTLEGAAAPFDILHFIGHGEYREQDDCGYLLFENAEGGVHELDSTTLRQIVCRRGIRL
                     VCLNACETGQGGREDFSRGVAQALIAGGVPAVVANQYPVLDVSATSFSRHFYWALAMG
                     QSIGDAAREARVAVNYSISGEAIDWAVPVVFARNPAQRICVPRPAAEYERTRAASERQ
                     RRRAMQDRIKIGMWNAHRMIPHLPAICDRLTKMQDVYSFETVSFPAPIGTWRREQDED
                     QAYVVAETLYERLKNKPRELGLDRLVCMINFPLRSGKKKNLYYWPLEPGKGERLSIVS
                     TFDLLDQLTGPEFTVERMMAHLAAAVVADLIPHLPGVGPADCPFFYNKDRDIRSIAGR
                     LRFCAACRRQCKNQEDQNRLRIAERLLAAYP"
     gene            complement(12740..13591)
                     /locus_tag="PKK81_05330"
     CDS             complement(12740..13591)
                     /locus_tag="PKK81_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32324.1"
                     /translation="MTPSISRPESSSSAAQEVVGLVPAAGLAKRLQPFPCSKEVYPVG
                     FVSDRQTGRPRPKVAAHYLLEKFRAAGITKAYLVIRDGKWDIPNYFREGGVVGLSLAY
                     IVIAGSLGPPDTIDRAYPFLEQKRVAFGFPDILFGPDDAYRQLIAAQERTGADVVLGL
                     HRVYDHRVWDMVDCDADGLVRNIVMKPVTTTLTYGWCCAVWTPAFSEFLHRFLRAEET
                     HRNLAQLANTAHDLGGDLAVGVVLQAALKAGLLLQSVIFPHDTYIDIGTPEDLAKAVR
                     QFNPPER"
     gene            complement(13588..14841)
                     /locus_tag="PKK81_05335"
     CDS             complement(13588..14841)
                     /locus_tag="PKK81_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV32325.1"
                     /translation="MNVSREEDPVPGDTNDEIRASLREHLAWWGLRHVESDAAYFAWQ
                     RDVFSPQELTTLHRHIEAKRQATDGPAAEIAFYDLTAQPRSVPALYSQRYEYYLEVGA
                     RVAGHLGDASTILDAGCGIGILTTFYAKRCPASTIVGIDRSPASIDLARQRAQELGLT
                     NVRFECRDVDQQDLAGCFNLIIATHTLLQAEQEPGLPSRDWSTFARPADAQAQQGFEQ
                     RIGVGVRLDRLRAGLTPGGRMVVFEKTRQLARRIPFQRALAARGLRLLAPPEPVRYRS
                     VEEVTDDGPLYVMGTVPREQACGWDESPEADGMPPLHLDSLRSARAQGDQPLYENHQI
                     SAEQGWLSLPARQVTAQVTSTEPDGRQLHVEWGRAGAFAYLYCANTFDQRQLVLIEPA
                     RAAVLETYYREITREQPAGGKEPLR"
     gene            complement(14852..16216)
                     /locus_tag="PKK81_05340"
     CDS             complement(14852..16216)
                     /locus_tag="PKK81_05340"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNV32326.1"
                     /translation="MVEVPVKLYLDKVLKSAREAVRPFSLMTGPARDKALRGMATAIA
                     EAEEDILAANEKDVDAVGKSMTGYENRERVRDAVARVRMTADDVKAMVDRLHRIADLP
                     DPLGEVLGRHDEPNGLQVSRVRVPIGVIGIVSELAPLETIDALALCLKSGNVCVFRGS
                     PDWTLTQQTIAACLSKAAADAGVPLGACTIIERPEKEAALELIKSGKALDAIIPRGGA
                     GLRKVVQEQAKMPILCHDGGITHVYIDGDVDIPLAQNIVVNSKVQQPLAANALDTLLV
                     HQGIARPLLSALILRLLDEFKIDVLGCPKTVALMGQMLMTGHKAVKPAQDADWNRQFQ
                     GPTMAIKMVPSFDDALAHITQHGPSHTCVIVTKSYESAMRFTKEVDAGSVLVNASSRL
                     NAGDSLGFGADVGLSSSRHHARGPIGLTQLTCEKYVVFGSGQLRHPHPVPLAYEDAIM
                     LKRP"
     assembly_gap    16366..16465
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16466..>17664)
                     /locus_tag="PKK81_05345"
     CDS             complement(<16466..>17664)
                     /locus_tag="PKK81_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV32327.1"
                     /translation="RRSVARFVQAEPQEIWPLAWSFGYFFCLLCGYYILRPVRDEMAI
                     QGGVHNLPWMMTGTFLTLLAVTPLFGWLSARYSRYRLLLAVYLFFIANLVGLYLLMTG
                     QQYIEWVARGFFVWLSVFNLFVVSVFWSFMADLFTPAQGARLFGMIAAGGSTGALFGP
                     LFTTGLTYLFPVPVLMLVSALFLLACIGCIYKLEAWSRGRTVSHRENSGEPLGGGFLA
                     GIRLVWSSPYLLGICGYLTFLTMTATFLYFEQVRLVAEHFDTPEARTRLFSTLDFATN
                     VLTWLTQLFITNRVVARYGLVASLLFLPVVSLIGFLGIALWPGLVVYVSFSVLRRVGE
                     YALSKPAREVLFTVVSREEKYKAKNFIDTAISRAGDASTGWLVSGIKALGVTTAQISW
                     VLVPLMF"
BASE COUNT         3532 a         5611 c         4957 g         3364 t
ORIGIN      
        1 gcaatggcca acgcgctgct cacgcgcccc tcccctggcg gcgcgccaaa tagtcggcca
       61 acgacatcga cggaggacgc gcggccgatt ttcgcccggg gggcgagggc ggaggcagag
      121 cggtgacctc gatccgtccg atcgtcacat gcacgggagg ctcgggagac gttagccctg
      181 cggcatcgcc cggtcgccct tgctgtcccg tggacaccaa cgaaggcgga gtgacgcggg
      241 ccgacatcgg cgtcgcctgt tgcgcctcga ctaaccgtgg cggaggcgtg atcgctctct
      301 cttgcgcagc ggttgcctca ctgcgccgtt cctccggcct tgcattgtcg cgggagaaca
      361 cacgagctgc atgccggtcc gattcgacga tctgattgcg cagggatacc ggcggccctt
      421 ctcccggggc tgcgcttggg acggccgtta ctttgacgac gggcgattcg ttcgacggct
      481 ccgtcccgcg cgcccctgtt tccggcagaa cgacagacgg ttcatcgaca ggagcttcga
      541 tgcgccgttg cgcatggagg aaggccgcca atggaggcat cggagcaatc gaatgacgcg
      601 gcgcctcggc cggttggcgc tgctcacccg acgaggccac cccgtcgatc accgaaagat
      661 cgggcgtcgg cggctccggc cgatccatcg caggggcaaa catggaacgg gtgcgcggtt
      721 gaatcgtagc gagcgttccg gcctgtcgtt ggacgagtct ggtgagaaaa tcgctcatga
      781 gagtgccatc cccacataca ggcggcgccg gtgatcggtc atgccgagga tctcgccttc
      841 cgtccagcca taggtgcgcg ccagcagatc aatttcctgc agcaaccgcc ggcctcgcac
      901 atggatctcg ttccagaaaa atcccgcgat atcgaagacg ctctgccacg cgtgcccgca
      961 ggcagcgcac ctcaagtcga tgaggatctc ggccaacgga tcgagctcgc tcagtcgcgc
     1021 cgccagggcc gcctgcaact catccgtcca atcagattcg tccaccgtgt caggtccgtt
     1081 gcgcaggcaa cgcgagagaa tcacacggcg cgcccgatct aagtccggag cggcggcggc
     1141 ggcggcaaga tcccggctgt tcggcccgcg cacatcccac gagcgccccg cttgctccac
     1201 cttgtgccag gtcgcgtccg tcatcgaagc cgatcccagc aaaccctcgc aggacagttc
     1261 aaattcgagt ccgttgcagc aggcaggaca ttccgcatac ccatcgatgc gatcgccgaa
     1321 ggtgtttcga cgggactcca acaaccgcct atcgcgctcc cccaccgtcc actcgcacag
     1381 ggtttcgaac ggctcgtcgg gacaggcata ccgcagcagc aacaacgcct ggtcgagggg
     1441 atgtcggccg gccccctgct cccagatgtc gatgaccgcc gctgcggaga gtcctgactg
     1501 ggatctgtgc gccatcgtca cggctccgta aaggtcggct ccgacggttc tgtgacttcg
     1561 tagtcgcgtt cccagccttc gttctcgagc ttcagggtct ggatcgcaac cgcattcgca
     1621 ttggcatcgc agtcagactg ggcttgatac tccgacaccc aacaacggaa gaccttgtag
     1681 gcgatggcca gttgtcccgc ctcgttgtac agttcgatga tgatgtcctt ccggaagtcc
     1741 ttgagcgata cttccgcgcc caacccggac ccgaagttcc agaccttgtt cgcccattgc
     1801 tcgaaggcgg tatcgtgagt gacaccccgc tccagcgtga tcgcttcata cttggtacga
     1861 ccgggcgatt tacggctggt gcttggatcg cccccttcgc ggtgctcgac cacttcggtg
     1921 gagcgcttca atgaaccgac cttgctgacc cccgcgacgt aacgcccgtc ccatttgacc
     1981 cggaatttga aattcttgta gggatcgaac cgatgcgtgt tgacggtaaa ctgcgccatg
     2041 ttttcctcct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctaggtcgc
     2161 gatctggccg gccatctgct ggagcttgat gatgacgaac tcagcaggct tgagcggggc
     2221 gaatccgacg acgatgttga cgatgccgag gttgatatcg ttctgggtcg tggtctcttt
     2281 gtcgcacttc acgaagtagg cgtcccgcgg tgcggtgcct tgaaatgcac cctgccgaaa
     2341 caggttgtgc atgaaggcgc ccaggttcag cctgatctgc gcccagaggg gctcatcgtt
     2401 cggttcgaac accacccact gcgtcccgcg atagagactt tcttcgagga acagggccag
     2461 ccgacgaacc ggcacatact tgtattcatc cgccgccgca tcggcgcccc gcatggtccg
     2521 ggaaccccat accactcgac cgtggaccgg gaatgtccgg agacaattga tacccagcgg
     2581 attcaagagt ccgttctccg catccgtcat atcgagctgc agcccggcga cgccgctcaa
     2641 cgaggcgtcg agccccgccg gcgccttcca cactccgcgt tggctgtccg ttctcgccat
     2701 cacaccggca atcacgccac agggcacgaa ggtctctact tgtcccccga gctgtgaatc
     2761 ggcctgcttg atgcgcggga aatagatcgc cgcgttgcgc gccatatcgc cgttcaggtt
     2821 catcccgggt gcccccgact gtgcagcgcc caccgtcgcc caggccgcct tcggatcgac
     2881 gatcagcatg gcccgccgct tgacacagta gctgagcgcc tcctgataca cgtcatccgg
     2941 cacatccccg ccgcgcgcgt cgggcaggat acagagcagg ttgaagagat ctattttttc
     3001 cagctggtag agtccggttt taccggtttg gcttcctttg taggccgccg catcgagcac
     3061 ggcgctctcg acggcaatgt ctgccggagc cgcattcttc gcaggcgtgg atttcgtggt
     3121 ggaagtccag acgtcggcat ccgccgaagt cccggcatgg gccgtgggcg agctattcga
     3181 cggcatcgtc gtcgtaacac ggaccagatt cgattccgcc aggagcacac ggtccgcgcg
     3241 acgcgcgctt tccttcgtcg tgagattcag gaatgtttcc gtgataccgg tgctcccgtc
     3301 gcggacaatc aagtcgaaca gatcggcggg tgccacgccg agcctggtgg cgacggcgat
     3361 cagattcgga tcggtcacag cctttttctc gacccgcgcg cgaacctgca tgccccacgc
     3421 gccttccgat gcagcctgca acgtcaggtt cggcacttca taggtcgcct tcgcggcact
     3481 ccccgcggtt gccttataga gacgcacgat cacggcttgt ccaccgccgt tcagaaaaaa
     3541 gtcacgcacg gcgtagctca aggtcatatc gcgatgcagt ccgccgaaga tccgctcgta
     3601 atccgaaaaa ctcgtgatcg tgaccggttg atcgaccggg ccgcgcacgg cctttcccag
     3661 aaaccccgtg atcgacgtgg cgactccggt gatcgtgcgc accccgctcg gcagctcctc
     3721 gatgtacaca cccggatagg ttggttgcac tggcatgctc atcctcctcg gcttaggacg
     3781 ttcacgtctc gtttccgtcc tgtgtcagaa atgttcgtat ctccttcagg tgcgcatgat
     3841 tcaggctata gatcggctga ttcgacgtgt cgcgtttcag gacccaacct tgcgcaacca
     3901 gcttgtccaa cgcctcgatc gcgagacgtt cagcaccttc atccaggcac ggtgcggtga
     3961 tccaccaatt cagcacccct tcctgagtgt ctttcgcgcc aggatgatga accaggtagt
     4021 cgagaatgct gcgcctgaac tctgcatcgt gctcttccat caggcctgat ccccggagcc
     4081 ccgctccatc acggcactcc tgcgggagtg agcaagagca atgccattag ggagaatccc
     4141 gtttcctgcg ttttaaaggg aatcgaacag gtgtaatcgg agggcgcggc attcctgccg
     4201 cagcagcacg gtgatacaaa gtttgaaacg gccttgaaaa tcagggcgat ggccggtgcg
     4261 gcaaaactac cgcggtggtg aggaacttag gcgcggccgt cgaacggtgt ctgaaagggc
     4321 gacggacaat cgcgcgagga atcggtcgga tcgttctgaa acgcttctcg atcgatcccg
     4381 tattttcgaa ggagtcgttg aaaactgcgc cgctccttgc ctgccgcttt ggccgcatgc
     4441 gtcacatttc cccggaatgt caccagagtc ttcacgagat aggcccgttc gacatccatg
     4501 gtcgcggagg ttttggccgc gcgaagcgag ccatccagac aggggctctt gggcgcctcc
     4561 cccgcacact cgatatcctg cgcccgaatc gtcgcggacc cgcacatgac gacggctcgc
     4621 tggagcgtac tctccaactc ccgcacattc cccggccagg catacgcgag caatttctgg
     4681 atggcgtctt cggacaaccg tcgcgggcct tgtccatccg ctcgccggaa gcgctggaga
     4741 aaccggtcgg ccagcaaggg aatgtcttcc acacgctccc gcagaggcgg caccaccagc
     4801 gacaacacat tgagccggtg gaagagatcc tcgcgaaact gccgctccgt cacggcctgc
     4861 cgcagatcat gattggatgc cgcgatgatc cgcacatgcg ccagcttcgt gcgcgaacag
     4921 cccaacggcc gatactcccg gtattgcacg aagcgcagca gcttcgcctg cgcgtatgga
     4981 ttcagcgcat cgacctcgtc gaggaacaac gttcccccct cagcctccag gaccagcccg
     5041 ccctgttcgc tggctgcact cgtataggcg cccttcacat gcccgaacaa ttcattttca
     5101 aacaactgat cgggcagtgc cgcacaattg atcggaataa acgcgtggtc cttcctggcg
     5161 ctggagtagt gaatcgcgcg ggcaaacact tccttcccgg tcccggtttc accctgcagc
     5221 agcaccgtcg catccacatc ggcgaagagg ggaatttttt caacctgtgt ctgaaagata
     5281 gaactctctc ccacaagcga atcgatcctc acgcgctgag accgaccgat accatgtggt
     5341 gttgaactcc gtccggctac gaggactcgc ccgattcgca gggccaattc gtctccatcg
     5401 accgggcaca agacaaaatc attcaactcc gccggaactc cgttcaggag cagtggacgg
     5461 cggccatgct ccgctgcaaa caatcctagt atcggcacat ccggccaacg accggccaca
     5521 tcggtcagcc tctgcgccga taaacgatca gtagatggat ggtgaaacaa cagcacgtgc
     5581 ggagagcgcg gaagcgttgc ggaaggatcc gacacccggt ggaactctat ggtccctcca
     5641 ggcagtgcac gttgcaaaac gtttacaagg tgagactcat cgaaccaggc atgatactga
     5701 ttgtccgaag tgaccaagag gattctgatg aagtccttca tccgactcac cctttgctgc
     5761 gagaagctta tctacgttca gccggcgacc ggccggcaga cacaaaccgc ctttctacgt
     5821 aaatgtgcac cattcgtacc atccgatgtg cgcagtgcaa cgcatgtatc agaacgagac
     5881 accgtcgaca aaccccgctc gatatacata ttctgaatgc accgggttgc gatgacgagt
     5941 ggcttcaacc gcagatgacc atactgctgg catcttcatg tatgtgattc gatacagtcc
     6001 ctcgtgattt tggatacagt ccgcaccacc ggtcagcatc gcccgctctt ataacacagg
     6061 acgccgaacc tgaccatgca cccgcaggat agaccgctcg attctccacg cgtagaatgc
     6121 acgctcgcac gggtgatgaa cagttcgacg ccatctacgt attcccaagc gtctttagca
     6181 cgacagcgga gaaccgcatg gcctatgaca gaggatccag agatgtcgca cctagcacgg
     6241 ggcactagcg caggaggagc tttcggacag catcggtcct ggtccttcct acttatgctc
     6301 ctcttatttt gtggaggctg ttcggaggtc cgggacagtc atgaactgct gcacagcctg
     6361 ctctggatgc gcacgtctgc tgagtaccat gtcctcagtg tcatgaccta tcaacgagcg
     6421 agggacgcgc tggatcgcgc gttgatcgac acgcgctcaa cagccagtat cgaacagacc
     6481 ggagactttc aacacctgcc tcccgccgtg atcctggatc tcgacgaaac agtgctggac
     6541 aacagcccct tcgaggcgcg gttgattgca cagcgcacca ctttcaatca gccgatgtgg
     6601 gaacaatggg tacaggaagc ctccgcgcaa gcggtgccgg gagtcctgga atttatcgcc
     6661 gaggctcgca agaaaggggt cgccttgttc ttcgtctcga accgcggggc tcaccaggaa
     6721 tcctcaacca ggcgcaacct cgagaaactc ggcattcacc ttccgatcga tcccgatgcc
     6781 ctgctgctcg aaggtgaacc tccgttccgg tggccgccga acaaatcgag ccggaggcga
     6841 tacctggccg agcggtaccg gatcctgctc ctcatcgggg acgatctggg agattttgtc
     6901 gacggggcac gggacaagcc ggagaatcga atcgcattgg ccagacaaca cgaccatcgg
     6961 tggggaaggt cgtggtttct ccttcccaat ccgatgtacg gtacctggga aacatcgctc
     7021 actccaccgg gtctctccga cactgaccgg ttgaccttca agcgggagct gatacgcaac
     7081 gctccttagc accccctctt gtctctctcc tgtataagac agattagttc agctgatgca
     7141 catcacgggc atgacccaaa atggaacatc gaatgcagcg tccaagaaaa ctgctcccgg
     7201 agggttgaca gctggaaggt gaataatgtg tactccgttc tggtcacgaa ttcctacacc
     7261 ataggatccc gagtgaccgg aacaaccaga gctctcagcc tgcaccaatt cccgattcac
     7321 tcacaaccgg gacattggcc atcctgacag tcggttcaac caattccgac atgtacgtcc
     7381 cgcgcatcag cgcagggtgg gaactgccca attcctgctc tggcctcggt atttgaagtg
     7441 acgaatacga tcgcaccaca acaacagagc ggtttcacga ggaggacgtc gttggatgac
     7501 ccgcgctgat aagcggacaa cgtcctgctc gccgaatctc gatttcctcc gcgcgagtgc
     7561 ggttttaatc gtctattttt ttcatctctt catcacgacg aacacccggt tgcctgatta
     7621 tctcggccag tttggcgtgc tgctcttctt cgtccatacc agtctcgtct tgatgttctc
     7681 gctggagcgg atcgaagcaa cagagggcag cttctttctg accttttatc tccgccggct
     7741 gtttcgcatc tatcccctca gtatcttctg tgtttccgtg atcgtcctct ttcaactgcc
     7801 ccgcgctccc tggtggccct ggtccaaccc ggacctgtcg accatcctgg ccaatctgtt
     7861 cctctatacg gagttcatgt acaaaccagt ggtcacctcc gtcctctgga gtctccccta
     7921 tgaggtggcc atgtacatag tactgccgtt cctctatctc gccgggaagg cctatggcgt
     7981 ccgtggaatt ttcgtactct ggtgcgtcgc tgtcgttggt gcgattgccc agccttacgt
     8041 gtcaagccgc ctcggggtgg cccaatatgc gccctgcttc atcgccggcg tggccagcta
     8101 cttcctgggg ttcgggatgc aacgattccg gctcccgttt atcggttggc ctctcactct
     8161 gctcatagcc ggcgctatct tggggtatgg aaatcactcc gaacatgaga tcaccgccca
     8221 gtgggccctg tgcgtgctga ttggtgtgac cgcgccgttc tttgccgagc tccagctacc
     8281 ggcaatgcgc aaggcctgcg ccctgattgc gcgatactcg tatggcatct acctcacgca
     8341 tttacatgcc cagtggaccg cgtttgtctt gctgaaggac tccccctcgg cggtacgtta
     8401 ttcggtgctc gtcgtcctga gcgtcggtct gccggttgcc ctctatcacc tgatcgaagc
     8461 cccgatggtc aggatgggag ccaggttggc ccagcggtta ccaagaactt ccgcacaatc
     8521 ctccaagccg acccccgctc aacggccatt ccccatcccg acatccgccg ccccgcactc
     8581 aacccaacac gcggcagcgc ggccgcgcgc agcccacgcg cccgatcacc gtcactccca
     8641 aaccacccct gcccgtgtct ccgcaagttg accggtttct cgcagccgaa cgcttcggcg
     8701 catcgaccgt atgagcctgc aacagatgac ggcaaaccga ttcatcgctg tcccacgcct
     8761 tttcctcgct cgattcaacg ttccagggca agatagggca agaatggaga ctatctattt
     8821 gggacagctg cctgaatgac cccataaacc attctcccca tcatcgtcgt tcctctccaa
     8881 tcgagcgcat cgtgcgcatg tatcgcaaag ttggccaacg agcaagcagg ctgatctgga
     8941 aacaacaccc gacttctcag cattctcccg ttcacatccc agttccccct gctctgctgt
     9001 cagcctcgtt tcgggcagcg taatacttgc atttctccct gcgccccaac gaatctcttg
     9061 cctcattttt ccggttatgg gataagcgca agcagttcgc gacggccacg agtcgaaccg
     9121 ccgtcgctcg tcactcgtga agcgtatctc gtttcaactt cgctcatctc aagcttcacg
     9181 agacacgagc gacgagctac aggatcacat gaatcgattg cgctcggtat gacgagcgaa
     9241 gtgggggcac atcatggcgc acaaaaaatc aggcacagtg agctggtcga caacagaggg
     9301 ctgggacgtt cgtgctacgg aacaggctcg tggagggcgt cgagcagcgg gcgaggtacc
     9361 tgaccccttc gtggccaacc tcaccgccac caagcgctgg gaagtcgagc aaaccctcac
     9421 cgcgacgccg aaggccagac gaggcgaggc ggctccggct cccctcaccc tggatgtcga
     9481 aggcgctcct gacgacgcgt acatcgtgat gacgcgatac gcctccggag ccatccgctt
     9541 ccacatgccg acggaaccgg ctcgacgcgg cattcgacga ggcgggaaga gaatctatcg
     9601 gttctcgatc ccggtgccgg ccgcgcccat cgccgaagca ggcggccgcc gcggattcat
     9661 cagcgcggcg atcaaggccg tcgtgctcaa agtggcaggg aaaattgccg actttgcgct
     9721 ctcgaaactc gcgctgctgt gggaaaccgc cacgtggaaa ttgaaaaaac aacgagaagg
     9781 gtggctgtcc ataaccgccg acagtctcgg cggagaatca ggcctgcctg ccgcagactt
     9841 gcagacgttg agcgcatcgg agcggaatct tctgttcatc cacgggacct tttccagcac
     9901 caaagcagcc ttccgctcgc tggcgcagac cccgggcagc aacggcagga cattttttga
     9961 agaactgcag gctgtgtatg gcaatcgcat ctatggattt gatcacttca ccctgagtcg
    10021 cacgccgcag gaaaacgtgc aaatgttgct cgaagccctc cccgctcgcc cgacgacctt
    10081 cgacgtgatc acccattcgc gcggtggcct ggtcctgcgg catgtggtgg aacgtcgcga
    10141 cctcttcggc tcgctggctg accggtttgt actgggccgg gccgtgctcg tcgccagccc
    10201 gaacgaaggc accccgttcg cctcacccga tcatgtgacc caatacacaa cctggctctc
    10261 caacgtcctt gaactgtttc ccgacaatcc cttcaccacc ggcgtagaat ttgtcagcga
    10321 agccctctcc tggatcgccc gccgtctggt gggcgggcta ccgggattgg cctcgatgga
    10381 caataagggt gacatcatcc aggcgttgca ggccacccca aaccctccca acgagaccta
    10441 ctcggctctg gtcgccaatt acgaaccgga cgcgggggtg ctgcagcgaa tcatcgatgc
    10501 gggagccgac ttattctttc cgacggccaa cgatctggtc gtgccgaccg aaggcgggtg
    10561 gcgggtggac gtcggaagcg gtgggacggt gacgattccg ggcactcgcg tcggctgctt
    10621 tggattaggc ggaaacctga cgcagcagaa ggcggtgaac catgtgaact tcttcgacga
    10681 tccgggcacg gtagactttt tggtgcgcgc cctgcgcggg gaggcccagc ctgccgcacc
    10741 gatcgacatc gagcacgacc tgccatccgg caagcggcgg ggcgccgttc gcaccgaagc
    10801 gcggcccacc atcgcggccg gtcctgcgct tcagcaaccg gcgacgatct ccccgactgt
    10861 ggctgcgact cctgcctcca aggcagtccc gcccattgta tccctgacgc agagccccga
    10921 gcctgacgac gtgttccaac tcgccctcat cgatcccgac ccgcgcgatg cggtcgcgac
    10981 gctcatcgcc accttccgca atgcgcgggt gatggaaacc gtgcacatcg ggacgggcag
    11041 cactgcgggc acagcccgcg acaaacagga tcacagatcg gcgcaatgga aaaacatcga
    11101 tgccatgcaa cagcacatcc acggctacat cgacggcgat ccgcgctatc ctcaactgcc
    11161 cggcgagaaa gacctccagc aattcggcgg cgcattgttc aatgcgctct ttcctgacca
    11221 ggtccgccgt ctctacgatg ccgcccggtc tgaacaggtc acgcaacggc tcaacctgat
    11281 ctttacctcg gacaatcatt ggctcgccag acaggcgtgg gaattcatct acgatcccga
    11341 tcgcagaacc ttcctggcgc tcgaggaagt gaattttaca cgcaacgtgc tcacatccat
    11401 tcccgccgaa cgcataccgg ctcgaaccgg ccctatgcgc atcctggtgg tcgtcgcaca
    11461 gccgttggga ttgggcgcct tgtccgtcga ggaagaaaca gacgtcattc gcagcggatt
    11521 ccggcggctt ctcgacgcga aactggcgga cgtggaactc ctgctcgacg ccacgcctga
    11581 gctgttacat cgaacgctcg aaggcgccgc cgcgccgttc gacattctgc acttcatcgg
    11641 acatggcgaa tatcgggagc aggacgactg cggctatctc ctcttcgaaa atgccgaggg
    11701 cggcgtgcat gaactggatt caaccacgct ccggcagatc gtgtgccggc gcggcattcg
    11761 tctggtgtgt ctgaacgcct gcgagaccgg acaggggggg cgagaagact tcagccgcgg
    11821 ggtggcgcag gccttgatcg ccggcggcgt cccggccgtc gtcgccaacc aataccctgt
    11881 gctggacgtg tcggcgacat ccttttcccg ccacttctat tgggcgctgg ccatgggcca
    11941 gtccatcggc gatgcggcgc gcgaagcgcg ggtggcggtc aattattcga tttccggaga
    12001 agccatcgat tgggccgtgc cggtcgtgtt tgcccgcaac ccggcccagc gcatttgtgt
    12061 tccaagaccg gccgccgagt atgaacgcac ccgggcagcc tcggaacgac agcgcagacg
    12121 cgccatgcag gaccggatca agatcggcat gtggaacgcg catcgcatga ttccccacct
    12181 ccccgcgatt tgcgatcgtc tgacaaaaat gcaggatgtc tattcgtttg aaaccgtctc
    12241 gttccctgcg ccgatcggca cctggcggcg tgaacaggac gaagatcagg cctacgtggt
    12301 cgccgaaacc ctctatgagc ggctgaaaaa caagccaaga gaactgggcc tggatcgact
    12361 tgtctgcatg atcaacttcc cgctcaggag cgggaagaag aaaaatctct actattggcc
    12421 actcgaaccg ggaaagggcg agcgcctctc catcgtgtcc acgttcgacc tgctcgatca
    12481 gttgacgggt cctgaattta cggtcgaacg gatgatggcc cacctggcgg ctgccgtggt
    12541 ggccgacctc atcccccatc tccccggcgt gggaccggcc gattgcccgt ttttctacaa
    12601 caaggaccgc gacattcgct cgattgccgg tcgcctgcgg ttctgcgcgg cttgtcggag
    12661 acagtgcaag aaccaggagg atcagaatcg cctcaggatt gccgagcggc tcctggcggc
    12721 ctatccgtaa gtgtcggagt tatcgttcgg gcgggttgaa ctgacgcacc gctttggcaa
    12781 gatcttccgg cgtgccgata tcgatgtagg tatcgtgggg aaagatcacg ctctgcagca
    12841 acagccccgc tttcaaagcg gcttgcagta ccaccccaac ggctaaatcc ccgcccagat
    12901 catgggcggt attggccaat tgagccaaat tgcggtgagt ctcctctgcg cgcagaaagc
    12961 ggtggagaaa ttccgaaaag gccggtgtcc agaccgcgca gcaccaaccg taggtcaacg
    13021 tcgtcgtcac cggtttcatc acgatattcc gcacgaggcc gtcggcgtcg caatccacca
    13081 tgtcccagac gcgatggtca tacacccgat gcagccccaa gaccacatcc gctccggttc
    13141 gctcttgcgc cgcaatcagc tgccggtagg catcgtccgg gccaaaaaga atatcgggaa
    13201 atccgaaggc cacacgcttc tgttccagaa agggataggc gcggtcgatg gtatccggcg
    13261 gtcccagcga gccggcgatc acgatgtagg cgagcgacaa ccccaccact ccgccctcgc
    13321 ggaaataatt cggaatgtcc catttgccgt ctcgaatcac cagataggcc tttgtaatcc
    13381 cggcagccct gaacttctcc aaaagataat gcgccgcgac cttcggacga ggcctcccgg
    13441 tttgccgatc cgacacgaag ccgaccggat agacctcctt actgcagggg aagggttgca
    13501 agcgttttgc cagcccggct gcgggcacca gcccgaccac ttcctgtgcg gccgatgaag
    13561 acgattcagg ccggctgatc gaaggcgtca tcgcaagggc tccttcccac ccgcaggctg
    13621 ttccctggtg atttcacgat agtaggtctc cagcacagca gcgcgggccg gctcaatcag
    13681 gaccaattga cgctggtcga aggtgttggc gcaatacaga tacgcaaacg cccctgcccg
    13741 cccccattcg acatgcagct gtcgaccatc cggctctgtg ctggtgacct gagcagtcac
    13801 ctgtcttgcc ggtaacgaga gccatccctg ttccgccgaa atctgatgat tttcgtacag
    13861 cggctggtct ccttgtgccc gagccgagcg gagcgagtcc agatggagcg gcggcattcc
    13921 atccgcttcc ggcgattcat cccacccgca tgcctgctcc cggggaacgg ttcccattac
    13981 atacaatgga ccgtcgtccg tgacctcctc cactgatcga tagcgcaccg gctccggcgg
    14041 tgcgagcaat cgaaggccgc gagcggcaag cgcgcgctga aatggaatgc ggcgggcaag
    14101 ttgtctggtt ttttcgaaga ccaccatgcg accaccggga gtcagcccgg cacgaagcct
    14161 gtccagcctg acgcccacgc cgatccgctg ttcgaatccc tgctgggcct gcgcatctgc
    14221 cgggcgtgcg aacgtgctcc agtcgcgact cggcagaccc ggctcctgct cagcctgcag
    14281 caacgtgtgg gtggcaatga tcaggttaaa acagccggcg agatcctgtt gatctacatc
    14341 gcggcattca aagcgtacat tggtcaggcc cagctcctgc gcccgctgcc gggcaaggtc
    14401 gatggaggcc ggcgagcgat cgatcccgac aatggtcgat gccgggcacc gtttcgcgta
    14461 gaaggtcgtg agaatgccga tgccgcagcc ggcatcgaga atcgtcgagg catcgccgag
    14521 atgaccggcc acccgtgccc cgacctccag ataatattcg tatcgctggc tgtagagcgc
    14581 gggaacagaa cggggctgag cggtcagatc gtagaacgcg atttcagcgg caggaccgtc
    14641 ggtggcctgc cgtttggctt cgatgtgacg atgaagtgtc gtgagctctt gcggggaaaa
    14701 gacgtcccgc tgccaggcaa aataggctgc atcggattcg acgtgccgga gtccccacca
    14761 ggctaagtgc tcacgcaggc tcgctcgtat ctcgtcattc gtgtcgccgg gcacagggtc
    14821 ctcttcacga gagacgttca cgatactcgc gttacggcct cttgagcatg atggcatcct
    14881 cgtaggccag cggcaccgga tgggggtgac ggagctgacc gctgccgaag acgacatact
    14941 tttcgcaagt gagctgtgtc agaccgatcg gccctcgcgc gtgatggcgc gaggaactca
    15001 acccgacatc ggcgccaaat cccaaactgt cgccggcatt caggcgcgac gaggcgttga
    15061 ccaggaccga accggcatcg acctctttgg tgaagcgcat agcggactca tacgatttcg
    15121 tcacgatgac gcaggtgtga ctcggcccgt gttgcgtaat atgagccagc gcgtcatcga
    15181 agctcggcac cattttgatc gccatggtag gcccctgaaa ctgccgattc caatctgcat
    15241 cctgcgccgg cttgacggcc ttgtgtccgg tcatgagcat ctggcccatg agtgccacgg
    15301 tcttcggaca gcccagcacg tcgatcttga attcatcgag caaccgcaga atcagcgccg
    15361 acaacagcgg cctggcgatc ccctgatgca ccaggagcgt atccaacgcg ttggctgcca
    15421 agggctgctg cactttggag ttcaccacga tattttgggc cagcggaatg tcgacatcac
    15481 cgtcgatgta cacatgcgta atgccgccgt cgtggcagag gatcggcatc ttggcctgct
    15541 cctgcaccac cttacgaagc cccgcgccgc cgcgcggaat gatcgcatcc agtgccttgc
    15601 cggacttgat cagttccagc gccgcctcct tctccggacg ctcaatgatc gtacaggcgc
    15661 cgagaggaac cccggcatct gcggcggcct tcgagagaca ggcggcaatg gtctgttgcg
    15721 tcagcgtcca atccggggaa ccgcggaaca cacagacgtt accggacttc aagcagagcg
    15781 ccagagcgtc gatcgtttcg agcggcgcca gttccgacac aataccgatc accccgatcg
    15841 gcacacgcac gcggctgact tggagcccgt tgggttcatc gtgacgtccc aacacctcac
    15901 cgagcggatc cggcagatcg gcgatccggt gcaaccggtc caccatggcc ttgacgtcgt
    15961 cggccgtcat acggacccgc gcaaccgcgt ctcggacccg ttcccggttc tcgtaccccg
    16021 tcatggactt cccgaccgca tccacatcct tttcattggc ggccaggatg tcctcttctg
    16081 cctccgcaat tgcggtcgcc atcccccgca atgccttatc cctggccgga cccgtcatga
    16141 gggaaaatgg acgcacggct tcacgggctg atttcaatac tttatcgaga tacagcttaa
    16201 cgggaacttc aaccatggtg ttcctatctt tcgacttaat acccggcgga ttcggcgatg
    16261 ttctcagcgg gcaatcggcc gccgcatcag cagatcagaa aatggtcact gcgtcgcgcc
    16321 ggactatacc atgcgatttt tttgtgagtc aaagacggag gggccnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnngaaca tgagtggcac cagcacccag gaaatctgtg
    16501 ccgtggttac gccgagggcc ttgatgcccg atacgagcca tccggtagag gcatcccctg
    16561 cgcgcgagat tgccgtatca atgaaattct tcgctttgta tttctcttcg cgactcacga
    16621 ccgtgaagag cacttcgcgg gcgggtttgg agagggcata ttcccccacc cggcgcaaca
    16681 cggaaaaact cacatacacg acgagccccg gccacagggc gatgccgaga aacccgatca
    16741 gactgacgac cggcaggaac aggagcgagg ccaccaggcc gtaccgggcc accacacgat
    16801 tcgtgatgaa caattgcgtc agccaggtga gcacattcgt cgcgaaatcc agcgtcgaga
    16861 agaggcgcgt cctagcttcc ggcgtatcga agtgctcggc caccagccgg acctgctcga
    16921 agtacaggaa cgtcgccgtc atggtcagga aggtgagata gccgcagatc cccagcagat
    16981 agggcgagga ccacaccagt cgaataccgg ccaggaaccc gccgcccaat ggttctccgc
    17041 tgttctcgcg gtgagacaca gtccggccac gactccaggc ctccagcttg taaatgcacc
    17101 cgatgcaggc caagagaaag agcgccgaga ccaacatcaa caccggaacc gggaagagat
    17161 aggtcagtcc cgtcgtgaac aggggaccga acaaggcacc ggtgctccct cccgcagcaa
    17221 tcatgccaaa gaggcgcgcg ccctgggccg gcgtaaacag gtcggccatg aaactccaga
    17281 acaccgacac cacaaacaga ttgaacaccg agagccacac gaaaaacccg cgggccaccc
    17341 actcgatgta ctgctggccg gtcatcagca gatacaggcc gaccagattg gcgatgaaga
    17401 acagatagac ggcgagcagc aatcgatagc gggaatagcg ggccgacaac cagccgaaca
    17461 gcggcgtgac ggccaacagc gtcaggaagg tcccggtcat catccagggc agattgtgca
    17521 caccgccctg aatggccatt tcatcgcgca cgggccgaag gatgtagtac ccgcagagga
    17581 gacagaagaa atacccgaag gaccaggcca gcggccatat ttcctgcggc tccgcctgaa
    17641 cgaaccgggc aactgaacgg cgca
//
LOCUS       DAOQRM010000042        17589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2318_length_17589_cov_16.675716, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000042 DAOQRM010000000
VERSION     DAOQRM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17589
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2318_length_17589_cov_16.675716"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1873)
                     /locus_tag="PKK81_05350"
     CDS             complement(2..1873)
                     /locus_tag="PKK81_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32328.1"
                     /translation="MKTHRALILGMLLIPLLASAGCESIKSRFARQALPDLGPPVPLT
                     IQMDVDPSLSAAKTEYIDGCGRIRPFSIGPTVEDMLIQAAHQTFRAVVLPGSHDSAGK
                     PDVTVRIRMLDPRFKIQPDALYDRAPAELSLDALAEFFDAAGAPLAERPLQSTRKERL
                     QLELTQQRCDYIVDPLLQDASTVLATQFMQEARVLLDPTHAATAAAPPASVVPTEAAQ
                     KPAGIVPANSTTTMASPLSFKATLLDENSNLILEGGERIRVRVDIVNTGSQAVQPMTV
                     QLTGPPALIAQFPATKLSTGTIETGGSKSLEFIATLPQSLNPQQAEFQVSLASGTGQP
                     VPAPQTLLASVRPTGITTDDVDQVPAPTTGGQRSGDYLVSIGISSYREQHIGGRKYAA
                     LDAEMVATYLQALGGVPKNNVRLLQDWSALRPDIEEALLDWLPSKLTQDSLVTVYFAG
                     QAVVSPTGDTFLIPYDGSLGSTSRLYPLKDLEAALERLKAKQILFVFDGTVLKNGGEG
                     RSKVAAPKWTGTSGKVLHLIGTTGFGKSLESDTLRHGLFTYYLLRGLRGEADLNRNGE
                     VTIGEVTDYLTRKIPTAARNTFKQEQQPQVFPALRAPDKGMDAVLTRPPTIPATDKP"
     gene            2036..3766
                     /locus_tag="PKK81_05355"
     CDS             2036..3766
                     /locus_tag="PKK81_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNV32329.1"
                     /translation="MVLGLSNMRDAAAALVSDGRIVAAAEEERFVRRKHVTALPVQAM
                     CYCLHEAGVALRDVEAIAVPWKYWQVGRRARLALTAMIRSPQLFRVKGTRSLERVSRE
                     WLELFRLREELTRRVDAGVIQPVFLDHHVCHAASSFLVSPFERSAILVVDGASEADTT
                     LMATGEGSRITVLDRTPLPHSLGQFYAAMTAFLGFRPDQDEYIVMGLASSGEPTFAPA
                     LRQNIVRLLSRGRFEVNVRLLDFHLARAGFFVEGFVRLFGAPRRSTDEITQRHRDLAA
                     SAQVLLEETLLHLGRHLRSLTQAESLCLAGGVAYNCVANGRLRAELGFKEVYVPPAAG
                     DSGAALGAALWWTARREGEAGRCRLPDAYLGPQFDEAACRAALSQAGLLAESLTDAQL
                     YERVAAELTRGRLVFWYQGRMEWGPRALGNRSLLADPRREDMRELINSKVKCRESFRP
                     FAPSVLADRAQEFFELPAPSPYMQFTVRVKASAKGLLPAVTHVDGTARVQTVTRETNQ
                     RFYDLLAAFGRLTGVPVLLNTSFNVQEPIVCTPEDAVRCFQRTQVEWLVLGNLLVGRS
                     SPPAISNHAC"
     gene            3756..5297
                     /locus_tag="PKK81_05360"
     CDS             3756..5297
                     /locus_tag="PKK81_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32330.1"
                     /translation="MPAEPVRFSDPSPHRLIVLLLDCLLVGFILNLTLQPLVEPDLGW
                     HLRAGLDLIAQGWRLPETDPYSHTMPDWHWIEHAWLTDGLLAVIYRVMDPLSGLGLIL
                     FFSGVTALAWWVAASQARVHRTYRLVAMVASLWVALPFLGARTQLVSLLGVAILLWGW
                     GRVQAGQRAWVWGLPPLLLLWANLHGGFTAGLFLSGVLLCISFMMTIGAGRPAVAASL
                     DEPVLSWDDLRRCALALGIAVAVTFLNPYGWRLYQEIYESLTDRFMIETLREWQPVSF
                     QGWAGKAYGMYLAGLACLMAGWYRRVEPVRWALLLLMLGLSLRHWRNVTLFLIVSLPL
                     VAELLALAVASCVRRVPILKTHSATALLLLTMGTAGALSALGSEHLEHVWLSGKSPDV
                     YLEQTEYPIEAVRWIRSHRQDVGTRLYNDYGYGGFLLWHFPGEKIFIDGRMPAWRIGD
                     RRIFQDYADLNREDAPRLAVLDRYAVDWGLLQRGSALALALEAHHAWERIYADAKVVI
                     VRRKG"
     gene            complement(5315..6118)
                     /locus_tag="PKK81_05365"
     CDS             complement(5315..6118)
                     /locus_tag="PKK81_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV32331.1"
                     /translation="MTPCSTQTPSLSIIIPAYNEARRLPLCLERVIAYLDQRGRTYEV
                     LVVDDGSHDQTAQAVESVAHRCPHLRLIRLTSNMGKGAAVRRGMQAARGTYQLFADAD
                     GATPIEELARLESALLAGADLAIGSRALASQDPAFTVRARWHRSLLGTVFNNIVQRLG
                     LRDITDTQCGFKLFRGSIAQDLFSVACVDGYAFDLELLYVARQRGYRLAEVPINWVDQ
                     PGSKVRPWRDGLAMLQELLAIRKRDEQGVYEPLIRPAPPALASVEPTHF"
     gene            6312..7085
                     /locus_tag="PKK81_05370"
     CDS             6312..7085
                     /locus_tag="PKK81_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32332.1"
                     /translation="MRRTQCRFMWWVGALLLAGCAVATPPSRLSQYVAPVVADESASP
                     GLPVQRPLRTALVVLADRSAQEAAPGLPEEAQARLAEALRGQVNRGFPIAIERVVNLG
                     EIPVGAPPPNWIDLAGQQGVDFVLVVILSSTEQEYPVSLFLGWTTHLQPGFRRDNWSL
                     AEAAFLDGRTGRQLLRAEGRAMATLDSPTAPGISQWYPVIYLRPQNPERRIWPPSYEG
                     APVTLRVVSMEQAATRLAGNLQRAWVEQRELELAALPGP"
     gene            7215..8750
                     /locus_tag="PKK81_05375"
     CDS             7215..8750
                     /locus_tag="PKK81_05375"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNV32333.1"
                     /translation="MTYRHRRRKVLSVLAGIPMIAATLLVGGMQPGSQAQAAGVPPAF
                     AQGFSEIVKAVTPAVVNIAVTGGGEGRREGRRQLPPGGPFGGPPPGPGDEPPGMEPPG
                     GPGGPPGPPGGGPHRPEQSAGSGVILDPNGYIVTNNHVVEGATQITVTLSDRREFPAK
                     IIGTDPKTDLAIIKIEAKDLASMKWADYDELQVGDVVLAVGSPFGLSSTVTLGIISAL
                     GRGNVGIADYEDFIQTDAAINPGNSGGALVNMQGKLIGINTAIFSRTGGSEGIGFAIP
                     SSIATDIVESLTKTGKVVRGWMGVAIQEITPALAKSFKLPEQRKGVLISDVNENGPSH
                     SAGMRRGDVVIAFNGKEVQSVSQLRNLVARMAVGKDADIKILREGKEQSLKVKVAERP
                     SDEVLAKREPGPAAPQTETVKPPDNVLASLRVQMLDAAMQSQLNIPAKTSGVVVSSVE
                     AGSAAESAGLQRGDVIQEVNHEVVKSLEDYQKASAKVKKDEMVVLLLSRQGNNLFVAV
                     NPK"
     gene            8831..9775
                     /gene="tatC"
                     /locus_tag="PKK81_05380"
     CDS             8831..9775
                     /gene="tatC"
                     /locus_tag="PKK81_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNV32334.1"
                     /translation="MADGFKFQEWLQDTVFKPLEDKKMPVMEHLVELQVRLTRAVIIT
                     AIIFVGTFFYADTLVKWIRVPLQNMFVPGSLSWIPTDLPTVPFVFLAPAEALWQNVKV
                     AGLFALVLGTPYILIEFWHFVVPGLHAQERRFVGPFVILSTLAFYLGLLFSFFFVLPF
                     ALNFLISYGVNAGFIPQLSIAQYVGFALWFLLVFGLIFEVPLVITLLAKLGWVDAPLL
                     KRYRKWAFLAAFIFAAILTPTPDPFNQCLMALPMYIFYEVGIVSAGIFNKKKAGAPEE
                     ALVPAVATAGPGLGKPGPSMPSGGGDGEYVGVPTGRRG"
     gene            9856..10812
                     /locus_tag="PKK81_05385"
     CDS             9856..10812
                     /locus_tag="PKK81_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNV32335.1"
                     /translation="MPRELNVISQPGSSSGGDACTYMWACAICDENETCQKDKEGHSR
                     WLVAKRMERIEYKVLVMSNKGGVGKSTCTTNLAVSLALKGWHVGICDMDIHGPNIPKM
                     VGAEGQKLKISTSGGIIPFQAYNMKIASMSFLLQNSDDPIIWRDAYKYEFINQLLGGV
                     EWQDLNFLLIDLPPGTGNESVTTIDLLGGVSGAVIITTPQEVALLDSRKSVTFCKDSE
                     VPIIGIVENMSGLECPHCHKEVNVFRKGGGEASATDMGVPFLGRIPLDPDVVTQSDAG
                     EPFALFNSDSATAQAYHDIANQVEAFCKKSGSLVKLAPRQQH"
     gene            10827..12125
                     /locus_tag="PKK81_05390"
     CDS             10827..12125
                     /locus_tag="PKK81_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNV32336.1"
                     /translation="MKAPDATTGLTPLHEAQRIVLDAATPLGLEKISILDTLGRVLGE
                     DIIAERHNPPWDNSAMDGFAVRAEDIQQEHTISKPVTLTVIEDVPAGKMPTKSVGRGQ
                     AIRIMTGAPIPKGADTVLKVEDTEHTPDSVRVFKPEPRGSNIRPQGEDVKKGDCIIPK
                     GTTIRSGEAGMLAILAKSFVLVYQRPRVAILSTGDELADLDERFSEEKIINSNSYGIA
                     AAVQEAGGVPILLGIARDTPAALKEKISHGLNADILVLSGGVSMGDYDFTKAVFRDLG
                     AEMNFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPAMLKLAGARGYG
                     RPLLEAVFQEKFSKRTDRRHFLRGVLWREDGVFKVRTTGDQGSGILTSMVKANCLIDI
                     PVEVERINPGDSVTVQLLSGSAWLEQAAPMASTGHRPSCC"
     gene            12146..12655
                     /gene="mobB"
                     /locus_tag="PKK81_05395"
     CDS             12146..12655
                     /gene="mobB"
                     /locus_tag="PKK81_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNV32337.1"
                     /translation="MSVPILSFVGRSNSGKTTLIERVIPELVRAGYKVATVKHAGHGF
                     DLDTEGKDSWRHKQAGASSVVIISKSSLAMFADVSDHMNVEDVRERYLDASYDLILAE
                     GWRSEGYPKIVVVRDQIGEVLVSQDGLLAVVSNKPVETSVPLLDPDDVAGVAALIIRH
                     FPKPQRDDA"
     gene            12645..13352
                     /locus_tag="PKK81_05400"
     CDS             12645..13352
                     /locus_tag="PKK81_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNV32338.1"
                     /translation="MMRKSSVIVLGAIVAGAVALGGVAVPLTNHPKFCASCHTIQPAY
                     ERWLESSHKEVECVACHVRPGVSGWLQDKAWHGTRDVAIYLFGKPTAPHNLQAHVDSG
                     VCLSCHRNILRVSEIATRDLPAPVKDVGLVMSHRKHMDAFGKRGQGEGCTTCHAAVVH
                     ERPIKGYPIVIPRGHVSADSRPWKPEHPEGSVLHKRAQADCFRCHDNKTEYEGRVLSR
                     KCETCHLPEKLTEFLSF"
     gene            13373..14101
                     /locus_tag="PKK81_05405"
     CDS             13373..14101
                     /locus_tag="PKK81_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV32339.1"
                     /translation="MSPSAVEVRNLTKRFGGFTAVDGISFDIGRGEILGLLGPNGAGK
                     TTTFQMMLGLVTPSSGSIRMFGLDLQTNREAILQQVNFSSTYISLPYSLTVEENLGVI
                     ARLYGMSNIAGRIDDMVKKLEMSEWRNKLTRKLSSGQMTRLTLAKALLTEPKVLFLDE
                     PTASLDPDIAHKIRAILLEERQATGLSILYTSHNMREMEEMSDRIIFLQRGKIVAEGT
                     AKDIVARFGQADLEEVFLKLAREQ"
     gene            14147..14617
                     /locus_tag="PKK81_05410"
     CDS             14147..14617
                     /locus_tag="PKK81_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32340.1"
                     /translation="MVDAGVSFGVGLLIGGLGLMLGWGLLWLVVGAIGLARETCGWAI
                     FMSSVSSTAIAGGAMAGVLWVVDSARLTSSSLHAGLAGVLLALLVAGFCRLEDGRRIG
                     PAFLEGSRLMLHQLLGVHEEGCGHCHEKPVEEPPCHQKPCYEQSCHEKPCEGKP"
     gene            14624..15415
                     /locus_tag="PKK81_05415"
     CDS             14624..15415
                     /locus_tag="PKK81_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV32341.1"
                     /translation="MAYHRIAALVIRHLYLYRRSLPRVMEIIYWPFLDLVVWGFITVY
                     LATFQGQMPAVVTFLLGALILWDVLFRSQQGITISFLEEIWARNLMNLFASPLTPSEF
                     LAATMVMSLFKVTAVSMVMSVCAWIFYGYNVFIIGLWLMPFIVNLVLTGWIIGVFTTS
                     LIMRFGQEAEVLAWSMVFLFQPISCVFYPMEVLPSWLRAVASINPAAHVFEGMRGLLA
                     THAAPLSSLSLASGLNLVYLGAVILWFHHTFSVCKDRGSLVRVGE"
     gene            15451..16125
                     /locus_tag="PKK81_05420"
     CDS             15451..16125
                     /locus_tag="PKK81_05420"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HNV32342.1"
                     /translation="MNINSGQVRPALLPVGLEAFSFHQTPVLVIENFWSAEERTQFRQ
                     AMNRANWNQLQDLSYVRQDFPNSGNWAKAEIAQPQGQLLLSRLEMPCIQQYIESFPNI
                     TRRHLGFSYYSYGAGDCLLTHDDTDQGHPVGGKPAPRRRLAMVSYFHEEWECDWGGEL
                     MIYSATGEPTNGKPELAITHCIAPKPGSLVMFTVPRFHRVCRVDQTAGDHKRLSIAGW
                     FMTEHA"
     gene            complement(16243..16851)
                     /locus_tag="PKK81_05425"
     CDS             complement(16243..16851)
                     /locus_tag="PKK81_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNV32343.1"
                     /translation="MEKKSKKKAPQESVATEDRKKTHVGDIVRRLRKSRHLSVRTLAD
                     KCGFSPSFISQVELRQASPSIASTERIASALGVTLGEFFRTTSPSHAAVIRADARPVV
                     ESEWSRARIEAIGPISEDSQLEPMVITLESGGASGSRPYVRRAEQLAVVLQGTVELTL
                     EENTYSLKRGDAACIPSDIHHCWRNTSRKPAQVLIVTAHRHL"
     gene            complement(17024..>17589)
                     /locus_tag="PKK81_05430"
     CDS             complement(17024..>17589)
                     /locus_tag="PKK81_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV32344.1"
                     /translation="IVQAGPAHEVVNHYLHAGHIQPVVEWLDPTQAPGNDVVRLRAVR
                     VRAESGIITDRFDIRKPIDLEVEFDVVKAGHIFVPVFNLYNEEDVLVFIAHDRDQAWQ
                     RTPRPTGRYTSTARIPGNWLAEGMMSVSSLMMTEDPFRMHAHAPRTIGFRVDDSGTGD
                     SARGDFHGRWPGVVRPLLEWRTKYLPA"
BASE COUNT         3276 a         4751 c         5707 g         3855 t
ORIGIN      
        1 ttcagggttt gtcggtcgcg ggtatcgtag gtggtcttgt aagtaccgcg tccatcccct
       61 tgtcgggggc tcgtagtgca gggaacactt gcggttgttg ttcctgcttg aaggtattgc
      121 gagctgccgt cgggatcttt cgggtgaggt aatccgtgac ctcgccgatc gtcacctctc
      181 cgttccgatt gagatccgct tcgccacgaa ggcctcgcaa gaggtaatag gtgaacaaac
      241 catggcgcaa cgtatcggac tccaggcttt ttccgaaacc cgtggtgccg atcaggtgta
      301 gtacctttcc gctcgtgccc gtccatttcg gtgccgcgac cttcgatcgt ccctcaccgc
      361 cgtttttgag aacggtcccg tcgaatacga acaggatttg cttcgccttc aatcgttcca
      421 gtgccgcctc cagatctttc agcggataca ggcgggaggt tgatcccagg gagccatcgt
      481 agggaatcag aaaggtgtct ccggtcggcg agacgacagc ctgcccggcg aagtacaccg
      541 tgacgaggga gtcctgcgtg agttttgatg gaagccaatc gagcaacgct tcctcaatat
      601 cggggcgtaa ggcgctccag tcttgcaaca accgcacgtt attcttgggc acccccccca
      661 gcgcctgtaa ataggtcgcg accatttcgg catccagggc ggcgtatttg cgcccgccga
      721 tgtgctgctc gcgatagctg ctgatgccga tggacaccag atagtcgccg gatcgctgtc
      781 cccccgtcgt cggagcggga acttgatcca catcgtcggt agtgatgccg gtcggccgta
      841 ccgaggccag cagcgtctga ggtgcgggga cgggttgtcc ggttccgctt gccagggaga
      901 cctgaaattc agcctgctgc gggttcagcg attgcggcag ggtggcgatg aactcgaggg
      961 acttggagcc gcctgtctcg atggtgcccg tggataattt cgtagccggg aattgtgcga
     1021 tcaacgccgg tgggccggtg agctgaacag tcatgggctg caccgcctgg gagccggtgt
     1081 tgacgatgtc gacccgtacg cggatccgtt cgccgccttc gagaattaag ttgctgtttt
     1141 catcgagaag ggtggcttta aatgacaggg gggatgccat cgtggtggtc gagttggcag
     1201 ggacgatgcc agccggtttc tgggcggcct cggtaggcac gacggaagcc ggtggggccg
     1261 cggccgttgc agcatgcgtg gggtcaagca gcacacgcgc ttcctgcatg aactgcgtgg
     1321 ccagcacggt ggacgcgtcc tgaagcaggg gatcaacgat gtagtcacag cgttgctggg
     1381 tgagctcaag ctgcagccgc tccttgcgtg tggattgcaa gggccgctcg gccagcggtg
     1441 cgccggctgc atcaaagaac tctgcgaggg catccaggct gagttcggcg ggggcacggt
     1501 catagagcgc atcgggttga atcttgaatc gagggtccag catgcgtatc cgcaccgtca
     1561 cgtccggttt tccggcgctg tcatgactgc cgggaagcac tacggcgcga aacgtctgat
     1621 gggcggcttg tatcagcatg tcttctacgg tcggaccgat agaaaacggc cggatccggc
     1681 cgcagccgtc gatgtattcg gtcttggcgg cggacagcga cgggtcaacg tccatttgaa
     1741 tggtgagggg gaccggaggg ccgaggtccg gcagggcctg gcgagcaaag cgggacttga
     1801 tactttcgca gcctgcggaa gccagcagag gtattaagag cattccaaga atgagtgcgc
     1861 ggtgcgtctt caagccgagg cctcctctac gccggatttt gaagcaggaa cggtacagga
     1921 aaggaggggg cattgcaacc gaatgccggt gcggcttgcg acgcgtacag gcctcgttta
     1981 ttctaccgcc gcttgtgtgt cggatcatga ccggaaggga ggctgattca tcgtgatggt
     2041 gttgggcctt tccaatatga gagatgcggc ggcggcgctc gtttctgacg gtcgcattgt
     2101 ggcggcggcg gaagaggagc gtttcgttcg ccggaagcat gtgacggcgc tgccggtgca
     2161 ggcgatgtgc tattgcctgc atgaggccgg agtcgcactt cgcgatgtcg aagccattgc
     2221 cgtcccttgg aaatattggc aggtcgggcg gcgcgcgagg ctcgccctga cggcgatgat
     2281 acggtccccg caactgtttc gagtgaaagg gactcgctca ttggagcgag tcagccggga
     2341 gtggctggaa ctgttccgat tgcgggagga actgacgagg cgcgtggatg cgggcgtcat
     2401 ccaaccggtc tttctcgatc accatgtgtg ccatgccgcc agttcgttcc tggtgtcgcc
     2461 cttcgagcgg tcggcgattc tcgtggtgga tggcgcgtct gaagccgata ccaccctgat
     2521 ggcgacaggt gaggggagtc ggatcaccgt gctggatcgc accccgcttc cccattcgct
     2581 cgggcagttc tatgcggcga tgacggcctt tctcgggttt cgccccgacc aagacgaata
     2641 cattgtgatg ggcctggcgt cctccgggga gccgacgttt gctcccgcct tgcgtcagaa
     2701 cattgtgcga ctactgtccc gaggtcgatt tgaagtgaat gtccgactgc ttgattttca
     2761 tctcgccaga gccggcttct ttgtggaggg attcgttcgc ctgttcggcg cgccgcggcg
     2821 ttcgacggat gaaatcaccc aacggcaccg cgatctggct gccagtgccc aggtgcttct
     2881 ggaggaaacg ttgttgcatc tcggccgcca cttgcgctcc ctcacgcagg ctgagtcgct
     2941 ttgtctggca ggcggggttg cctataactg tgtggccaat gggcggctgc gcgccgaact
     3001 cgggtttaag gaggtctatg tgccgccggc cgcaggcgat tcgggtgcgg cgcttggggc
     3061 ggccttgtgg tggacggcga ggcgtgaagg agaggcggga aggtgtcgct tgcccgatgc
     3121 gtatctgggg ccacaattcg atgaagctgc ttgtcgggca gcgctgtcgc aagcaggcct
     3181 gctggccgag tccctgacgg atgctcagct ctacgagcgg gttgccgccg agctgacacg
     3241 ggggcgtctc gttttctggt atcaaggccg gatggagtgg gggcctcgcg cgctggggaa
     3301 tcgcagcctc ctggcagatc cgcggcggga agacatgcgg gagttgatca acagcaaagt
     3361 aaaatgccgg gagtcgtttc gacccttcgc tccctcggta ctggctgatc gggcccagga
     3421 gttctttgaa ctgccggcac catcgcccta catgcagttc accgtgcggg tgaaggcctc
     3481 ggcgaaagga ctccttccgg ccgtcacgca cgtcgatgga acggcccggg ttcagacggt
     3541 gacgcgtgaa accaatcagc ggttttatga tcttctggca gcgttcgggc gacttacggg
     3601 tgtcccggtg ctgctgaata cgtcgttcaa tgtgcaggag ccgatcgtct gtacgcccga
     3661 agatgcggtg cgctgcttcc agcggactca ggtggagtgg ttagtcctcg gcaatctact
     3721 ggtgggcaga tcatctccac cggccatctc gaatcatgcc tgctgaaccg gttcgttttt
     3781 cagatccttc ccctcatcgc ctgatcgttc tcttgctcga ttgtcttttg gtcggcttca
     3841 ttctgaatct cacgctgcag ccgctggtag agccggacct gggatggcat ctgcgcgccg
     3901 ggctggattt gatcgcccag ggctggcggt tgccggagac cgatccctac tcccacacca
     3961 tgccggattg gcattggatc gagcatgcct ggttgaccga tggcctgctg gcggtgatct
     4021 accgtgtgat ggacccgttg agtggtctgg gtctgatcct gttctttagt ggcgtgacgg
     4081 cgctcgcctg gtgggtggca gcatcgcagg ccagagtgca tcggacgtat cgcttggtgg
     4141 ccatggtggc gagcctttgg gtggcattac cctttctagg cgcgcgcacg caactggtca
     4201 gtctgctcgg cgtcgcgatt ctgttgtggg gatggggccg cgttcaggct ggacagcggg
     4261 catgggtctg gggactgccc ccgttgctcc tgctttgggc gaacctgcat ggcgggttta
     4321 cggcgggact ttttttgtcc ggtgtgttgt tgtgcatctc cttcatgatg acgataggcg
     4381 ccggacggcc ggcggttgcg gcgtcgctcg atgagccggt cctcagttgg gacgatcttc
     4441 gccgctgtgc gctggcgctg ggcattgcgg tggcggttac cttcctgaat ccttatggat
     4501 ggcgtcttta ccaggagatt tatgagtccc tgaccgatcg gttcatgatt gagacgttac
     4561 gggaatggca gccggtgtct ttccaggggt gggcggggaa ggcctacggg atgtatctgg
     4621 ccgggctggc gtgcttgatg gccggatggt atcgccgggt agaaccggtt cgatgggccc
     4681 tgctgctgct catgttgggg ttgtcgttgc ggcattggcg gaacgtcacc ctgttcctga
     4741 tcgtgagcct cccgctagtg gcggagttgc tcgcacttgc ggtggcctcg tgcgttcgga
     4801 gggtaccgat tctgaagacg cactctgcga cggctctgct tctgttgacc atggggacgg
     4861 ccggtgccct ctctgctttg gggagcgaac atctggagca tgtctggtta tcggggaagt
     4921 cgcctgacgt gtatctggaa cagacggaat atccgattga agcggtgcga tggatccgga
     4981 gccatcggca ggatgtggga accaggctct ataacgatta cggatacggc gggtttctgc
     5041 tctggcactt tcccggtgag aaaatattca tcgatggtcg tatgccggcc tggcgaatcg
     5101 gcgaccggcg catctttcag gattatgcag acctgaatcg cgaggacgca ccacgtttgg
     5161 cggtgctgga tcggtacgca gtcgattggg ggctgctcca gcggggcagc gcgcttgcgc
     5221 tggccttgga ggcgcatcac gcctgggaga ggatctatgc cgatgccaag gtggtgatcg
     5281 tgcgacgaaa gggatgatca gttggacggg tctgctaaaa atgggtgggt tcgacggaag
     5341 ctaaggctgg cggtgcggga cgaataaggg gctcgtatac cccttgctcg tcacgtttcc
     5401 ggatggccag gagttcttgc agcatggcga gtccgtcgcg ccaggggcgg actttcgagc
     5461 ccggctggtc gacccaattg atcgggactt ccgcaagccg gtagccgcgc tgtcgcgcga
     5521 cgtagagcag ctccaggtcg aacgcatagc cgtcgacaca ggcgaccgaa aacaaatctt
     5581 gagcgatcga tccacgaaac agcttgaatc cgcattgagt gtcggtgata tcgcgaagac
     5641 ccaggcgctg cacgatgttg ttgaagacgg ttcccagcag gcttcgatgc caccgtgccc
     5701 ggacggtgaa ggcgggatct tgggacgcca acgcccgtga tccgatggcg agatccgctc
     5761 ctgccagaag cgccgattcc agccgtgcca gttcttcgat cggtgtggcc ccatccgcgt
     5821 ccgcaaacaa ttgatacgtg ccccgcgcgg cctgcatgcc acggcggacg gccgcgccct
     5881 tgcccatatt gctggtcaat cgaatgagtc gcaggtgcgg gcagcgatgg gccaccgact
     5941 cgacggcctg cgcggtctgg tcgtggctgc cgtcatcgac gacgaggact tcataggtcc
     6001 ggccgcgctg atccaggtag gcaatgactc gttccaggca gagtggaaga cggcgcgcct
     6061 cgttataggc cgggatgatg atcgaaagag aaggcgtctg agtcgagcac ggtgtcacgt
     6121 tgggacagcc tcgtgtcgga gtatgacggg accgatccgt ctgttgaagg cgaatggtgg
     6181 cccacggtat ctgaccgagg ggcgaaatgc aagccgtggg tgccgggtct tgacagagcc
     6241 atcgaccggg ggtgatcgta atctcacaga ctgtaggtgc ccaggcgtcg ctctctggcc
     6301 aggaggttgc gatgaggcgg acacagtgca ggtttatgtg gtgggtcggg gcgctgttgc
     6361 ttgccggttg cgccgtggcg actccaccca gccggttaag tcagtatgtc gcccctgtcg
     6421 tggcggacga atcagcaagc cccgggcttc cggtgcagcg cccgcttcga accgcgctgg
     6481 tcgtgctggc cgatcggtcg gctcaggagg ctgccccggg tttgcctgaa gaagctcagg
     6541 caaggctggc tgaggcgctc agggggcagg tcaatcgggg cttccccatc gccattgaac
     6601 gggtggtgaa tctcggtgag attccggtcg gagcgcctcc tccgaattgg atcgaccttg
     6661 ccgggcagca gggtgtggat tttgtcctgg tcgtgatcct ttcgagtacg gagcaggagt
     6721 atccggtatc gctgttcctc ggctggacga cccacctgca gcccggattc cgtcgcgaca
     6781 attggtccct cgcggaagcc gccttcctcg atggacgaac aggtcggcag cttctccgtg
     6841 cggaggggcg agccatggcg acgttggaca gccccacggc gccagggatt tcgcagtggt
     6901 atccggtgat ttatctgcga ccgcagaatc cggagcgacg catctggccg ccatcctatg
     6961 aaggcgcccc tgtgacgctc cgtgtggtgt cgatggagca ggcggcgacg cgattggccg
     7021 ggaatctgca gcgagcctgg gtcgaacagc gggagctgga gttggccgcg ctgcccggac
     7081 catgactcct gtccaccgga gcaacgggcc ctgctttttc ctctttgaag gcgattctgt
     7141 gattagtgta gaatgattcg ttcacgatgg cgcggaggat ggtccctcat tttcaggctt
     7201 ccagaggagt acacatgacg tatcgacatc ggcgcaggaa agtgctgtcc gtactggcag
     7261 ggatccctat gattgctgcc acgcttttag tcggcggaat gcagcccggg tctcaggctc
     7321 aggcggcggg tgtccccccg gcgtttgcgc agggattctc agaaatcgtc aaggcggtca
     7381 cgcctgcggt cgtcaacatt gccgtcaccg gtggagggga aggccgtcgt gaaggccgtc
     7441 gtcagcttcc tccaggcgga ccattcggtg gcccgcctcc tggacccggt gatgagccgc
     7501 ccggcatgga accgccaggt ggtcccggtg gacctcccgg ccctccgggt ggtggccccc
     7561 atcgccccga gcagagtgcc ggatccggtg tgatcctcga tccgaacggg tatatcgtca
     7621 cgaataacca cgtggtcgaa ggcgcgaccc aaatcaccgt gaccctgtcg gaccgtcgtg
     7681 aatttccagc caagatcatc ggaaccgatc cgaagacgga tttggccatc atcaagattg
     7741 aagccaaaga tctcgcgtcc atgaagtggg ccgattatga tgaattgcag gtcggtgacg
     7801 tggtgctagc ggtgggcagc ccgttcggac tgagctcaac cgtgaccttg ggcattatca
     7861 gcgctctggg acgcgggaac gtgggcattg ccgattacga agactttatt caaaccgatg
     7921 cggcgatcaa ccccgggaat tccggcggcg ctttggtcaa tatgcagggc aagttgatcg
     7981 gcatcaacac agcgatcttc tcccgcaccg gcgggtccga gggcatcggc tttgccattc
     8041 cgagcagcat cgcgaccgat atcgttgaga gtttgacgaa gaccggcaaa gtggtgcgcg
     8101 gatggatggg tgtggccatt caggaaatta ccccggcgct ggcgaaatcc ttcaaattgc
     8161 cggagcagcg caaaggcgtg cttatcagcg acgtgaacga gaatgggccc tctcattcgg
     8221 ccggcatgcg gcgtggtgac gtggtcattg ccttcaatgg taaagaggta cagagcgtca
     8281 gccaattgcg gaacctcgtg gcccgtatgg ctgtcggcaa ggatgccgac atcaagattt
     8341 tgcgcgaagg gaaagagcag agcttgaagg tgaaggtggc tgagcggccg tcggacgagg
     8401 tcctcgctaa gagggagccg ggtcctgccg cccctcagac agagaccgtg aagccgccgg
     8461 acaatgtcct ggcgtccttg cgcgttcaga tgcttgatgc cgcgatgcag agccagctga
     8521 atattcccgc aaaaaccagc ggcgtggtgg tcagttcggt tgaggccggc agcgccgccg
     8581 agtctgcagg gttgcagcgt ggcgatgtga ttcaagaggt caatcacgaa gtggtcaaga
     8641 gccttgagga ttaccaaaag gcatcggcca aggtgaagaa agacgaaatg gttgtgctct
     8701 tgctgagtcg gcaggggaac aatctgttcg tagccgtcaa cccgaaatag acagcaacca
     8761 gcggacagct ttcagctatc aggccaattc cggctgatgg ctgaaggcct cctgctgaaa
     8821 gcgattttga atggctgacg ggttcaagtt tcaagagtgg ctgcaagata cggtctttaa
     8881 gccgcttgaa gataaaaaaa tgccggtcat ggagcacctg gtggagctcc aggtccggct
     8941 gacccgtgcg gtcattatca cggcgatcat ttttgtcggc acattcttct acgccgacac
     9001 cctggtgaag tggatcaggg ttccgctcca gaatatgttc gttcccggat ctctttcctg
     9061 gatccccacc gatcttccca ccgtcccctt cgtgtttctc gcgccggctg aagcgctgtg
     9121 gcagaacgtc aaggtggccg gcctctttgc cctggtattg gggactccgt atattctcat
     9181 tgagttctgg cattttgtgg tgccgggtct gcacgcgcag gagcggcgat tcgtcggtcc
     9241 gtttgtgatc ctgagtacgc tggcgttcta cctgggcctg ttgttctcgt ttttcttcgt
     9301 gctccccttt gctctgaact ttttgatttc gtatggcgtg aatgcgggct tcattccgca
     9361 actgtcgatt gcgcaatatg tcggattcgc gctgtggttt cttctggtct tcggtctcat
     9421 ttttgaagtg ccgttggtga ttaccctgct ggccaaactt ggctgggtgg atgccccact
     9481 gctgaaacga tatcgcaagt gggccttcct cgcggcgttt atcttcgccg cgattctgac
     9541 gccgacgccg gaccccttca atcagtgcct gatggcgctg ccgatgtata ttttttatga
     9601 ggtcgggatc gtgagtgccg gaatcttcaa caagaagaag gcgggcgctc ccgaagaggc
     9661 gcttgtgccg gcggttgcca cggcgggtcc cggtctcggg aagccgggac cgtccatgcc
     9721 gtccggcggc ggtgatggtg aatatgtcgg tgtgccgact ggtcgtcgag ggtaacgcag
     9781 ggcaggaatc agcaacgtga cgaacgcaca tgtttgtccc acgcatcatg atatacgctt
     9841 tatgaggagt ctaggatgcc acgtgagtta aacgtcattt cacagcccgg ttccagcagc
     9901 ggcggagatg cctgcaccta tatgtgggcc tgcgccattt gcgacgagaa tgaaacctgt
     9961 cagaaagaca aggaaggcca cagccggtgg ctcgtcgcca aacgcatgga acggatcgaa
    10021 tataaggtcc tggtcatgag caacaagggt ggagtgggga aaagcacctg caccaccaac
    10081 ctggcggtca gcctcgcgct caaaggctgg catgtcggga tttgcgacat ggacattcat
    10141 ggtccgaaca ttcccaagat ggtcggggcg gaaggacaga agctgaagat cagcacgtcc
    10201 gggggaatca ttccgtttca agcctacaac atgaagatcg cgtcgatgtc gttcctgttg
    10261 cagaactccg atgatccgat catctggcgc gacgcttata agtatgagtt catcaatcag
    10321 ttgctgggag gggtggaatg gcaggatctc aatttcctgt tgatcgatct gcccccaggc
    10381 accggcaacg aatcggtgac gactatcgat ctcttgggcg gggtcagcgg agcggtgatc
    10441 attacgacgc cgcaagaagt cgcgctgctc gattcccgca agtccgtgac cttctgtaaa
    10501 gacagcgaag tgccgattat cggcatcgtc gagaacatga gcgggctgga atgtcctcat
    10561 tgccacaagg aagtgaatgt cttccgtaag ggcggcggtg aagcgtcggc tactgatatg
    10621 ggcgtcccgt tcctgggccg gattccgctc gatcccgatg tcgtgacaca aagtgatgcc
    10681 ggtgagccat tcgcgctctt taattccgat tcggcgaccg cgcaggccta tcacgacatc
    10741 gcaaatcagg tcgaagcgtt ctgcaagaag agcggatcgc tggtcaagct ggcgccgcgt
    10801 cagcaacatt gatgggacgg tgacgcgtga aagctcccga tgcgacaacc ggattgactc
    10861 cgctccacga agctcagcgc attgtgctgg acgccgcgac tccgctgggg cttgaaaaga
    10921 tctcgattct ggataccctg gggcgcgtgc tcggcgaaga tatcatcgct gagcgccata
    10981 atccgccgtg ggacaattcc gcgatggacg gattcgcggt gcgggcggag gacattcagc
    11041 aggagcacac gatctccaag ccggtcacgt tgacggtgat cgaggatgtg ccggccggaa
    11101 agatgcccac caaatccgtg gggcggggcc aagccatccg gatcatgacc ggcgcgccga
    11161 ttccgaaggg cgcggatacc gtactcaagg tcgaggacac ggaacatacg cccgattcgg
    11221 tgcgcgtctt caagccggaa ccccgtggct cgaatatccg gccgcagggg gaggatgtga
    11281 aaaagggcga ttgcatcatt ccaaaaggaa cgacaatccg ttccggcgaa gcggggatgc
    11341 tggccattct ggccaaatct ttcgtgttgg tctatcagcg gccgcgagtc gcgattttat
    11401 cgaccggcga tgagttggcc gatctcgatg agcgcttcag cgaagagaaa atcatcaact
    11461 cgaacagcta cgggattgcc gctgcggtgc aggaagccgg tggcgtgccg atcctgttgg
    11521 ggattgcgcg ggacacgccg gccgcgttga aagaaaagat ttcgcacgga ttgaatgccg
    11581 atattctcgt gctctcaggc ggcgtgtcga tgggggacta cgacttcacg aaagcggtct
    11641 tccgggattt aggcgccgag atgaatttct ggaagttggc gatccgccca ggtcagccgc
    11701 ttgccttcgg aaagattcag ggcaaactgg cattcgggtt accggggaat cctgtttcct
    11761 cgatggtgac cttcgagcaa ttggttcggc ctgccatgct gaaactggcc ggggcgcgag
    11821 gatatgggcg tccgctgctt gaggcggtgt ttcaggagaa gttttcgaag cgaaccgatc
    11881 gccgtcattt tctccgcggg gtgttgtggc gggaagacgg agtcttcaaa gtccggacga
    11941 ccggcgatca ggggtcaggc attctcacct ccatggtgaa agccaattgt ctgatcgaca
    12001 ttcccgtcga ggtggaacgg atcaatcccg gcgactcagt caccgtacag cttctcagtg
    12061 gatcggcatg gctggagcaa gcggccccga tggcatcgac cggtcatcgt ccttcctgtt
    12121 gttgagccgc gaaaggaaca tgttcatgtc cgtgccgatt ttatcttttg tcggacgatc
    12181 caatagcggt aagacgacgc tgatcgagcg tgtcatcccg gaactggtgc gggcggggta
    12241 taaggtggcg accgtcaaac atgccgggca tggattcgat ctggataccg aaggaaaaga
    12301 cagctggcgg cacaaacaag ccggggcgag cagtgtggtc atcatttcca agagcagcct
    12361 ggccatgttc gccgatgtgt cggaccatat gaacgtcgag gatgttcgtg aacggtatct
    12421 ggatgcgtcc tacgacttga tcctcgctga aggctggcgg agcgaagggt atcccaagat
    12481 cgtagtggtc cgcgatcaga tcggcgaggt gctggtttcg caggatgggc tactggctgt
    12541 ggtgtccaat aagccggtcg agacctcggt cccgctcctc gaccccgacg atgtcgccgg
    12601 cgtcgcggca ttgatcatcc gccactttcc caaaccccaa cgggatgatg cgtaaatcct
    12661 ccgtcattgt gctcggagcg atcgtggccg gcgcagtggc tttaggggga gttgcggttc
    12721 cgctgaccaa ccacccgaaa ttttgtgcca gctgtcacac cattcagccg gcctatgagc
    12781 gctggctgga atcatcccat aaagaggtgg agtgtgtggc gtgccatgtc aggcccggcg
    12841 tctcaggttg gttgcaggac aaggcgtggc atggaaccag ggatgtggcc atctatctct
    12901 tcggcaaacc gaccgcgccc cacaatctgc aagcgcatgt cgattcgggc gtgtgtctga
    12961 gttgccatcg gaacatcctc cgggtgtcgg agattgcgac tcgggatctg cccgcacctg
    13021 tgaaggatgt gggattggtc atgagccatc gcaagcatat ggacgcattc gggaagcgag
    13081 ggcagggcga gggctgcacg acctgtcacg cagcagtcgt gcacgagagg ccgatcaagg
    13141 gctaccctat cgtgattcct cgcgggcacg tgtcggccga tagccggccc tggaagcctg
    13201 aacatccgga aggttccgtc ctgcacaagc gggcgcaggc ggattgcttc cgctgccacg
    13261 ataataaaac cgagtatgaa ggtagagtcc tgagccggaa gtgcgagacg tgccatctgc
    13321 ctgagaaact cacagaattc ttgtcgtttt aatccacggg aatactcgct ccatgtcgcc
    13381 ctcggccgtc gaagtgcgca atctgaccaa acgcttcggc gggttcacgg cagtggacgg
    13441 gatttcgttc gacatcggcc ggggtgagat tctcgggctg ttgggcccga atggagcagg
    13501 aaaaacgacg acgtttcaga tgatgctggg actggtcaca cccagttccg gctcgatccg
    13561 gatgttcgga ctcgacttgc agaccaatcg ggaggcgatt cttcagcagg tgaatttttc
    13621 ctccacgtac atctcgttgc cctattccct gacggtggag gaaaatctcg gtgtcatcgc
    13681 tcggttatac gggatgtcca acatcgccgg ccgaatcgat gacatggtga agaagctgga
    13741 gatgagtgag tggcgaaaca agctcacccg caaactttcg tcggggcaga tgaccaggct
    13801 cacattggcc aaagcgttgc tcacggagcc caaggtgctg tttctcgatg agcccacggc
    13861 aagcctcgat ccggacatcg cgcacaaaat ccgcgcgatc ctcctcgaag aacggcaggc
    13921 caccgggctc agcattctct acacctccca caacatgcgg gagatggagg aaatgtcgga
    13981 tcgtattatc ttcctgcaac ggggcaaaat cgttgcggag ggcacggcga aggacattgt
    14041 cgccagattc ggacaggccg atctggaaga ggtgtttctc aagttagctc gcgagcagta
    14101 acggcagatc gggatcgctg cttcgtggaa gggagtacgg gcagtcatgg ttgacgcagg
    14161 agtgtccttc ggagtgggtc tgctcatcgg cgggttgggc ctgatgctgg gatggggatt
    14221 gttgtggctc gtcgtcggcg ccattggtct ggcacgagag acgtgcgggt gggcgatctt
    14281 catgagcagc gtcagcagca cggccattgc ggggggggcg atggcgggcg tgctctgggt
    14341 ggtggattcg gcgaggttaa ctagttcttc gctgcatgcc ggtctggccg gggttctgct
    14401 ggcgctgctt gtcgcagggt tctgtcgttt ggaggatggt cggcggatcg gtccggcgtt
    14461 cctggaaggc agccgcctca tgttgcatca actgcttggt gtgcatgagg agggatgcgg
    14521 gcattgccac gagaaacctg ttgaggagcc gccctgtcac cagaaaccct gttacgagca
    14581 gtcctgccac gagaagcctt gtgaagggaa gccctagctg tcgatggcct atcatcgtat
    14641 tgcagccctg gtgattcgtc acctgtatct ctaccgtcgc agtctcccgc gggtgatgga
    14701 gatcatctac tggccgtttc tggacctggt ggtctgggga ttcatcaccg tctatctggc
    14761 gacttttcaa gggcagatgc cggcggtggt cacgttcctc ctcggggcgt tgattctctg
    14821 ggacgtgtta tttcgctcgc agcagggtat taccatttcc tttctcgagg aaatatgggc
    14881 gcgtaatctg atgaacctct tcgccagccc gctcacgccg agtgaatttc tggcggcgac
    14941 catggtgatg agcctgttta aagtgaccgc ggtgtcgatg gtcatgtcgg tctgcgcctg
    15001 gatcttttat gggtacaacg tctttatcat cgggctctgg ctgatgccgt tcatcgtgaa
    15061 tctggtgctg acaggctgga tcatcggtgt gttcacgacc tcgctgatca tgcggtttgg
    15121 gcaggaggcg gaagtgttgg cctggagcat ggtctttctg tttcagccga tctcctgcgt
    15181 attttatccc atggaggtcc tgccgtcctg gctgagagct gtggcttcca tcaatccggc
    15241 cgcccatgtg tttgagggca tgcgcggcct gctggccacc catgcggctc ccctgagcag
    15301 cctgagcctg gccagcgggt tgaatcttgt gtatcttgga gcggtcatcc tatggtttca
    15361 tcacaccttc agtgtgtgca aggaccgggg atcgttggtg cgggtcggtg agtaggtgat
    15421 cgaccgaaca gggaatgtgg gagtagtggg atgaatatca actcagggca agtcaggccg
    15481 gcactgctgc cggtcggtct cgaagccttt tcgtttcatc agacgccggt cctcgtcatc
    15541 gagaattttt ggtccgccga ggagcggact cagtttcggc aggcgatgaa ccgtgcgaat
    15601 tggaatcagt tgcaagattt gtcgtacgtg cggcaggact tccccaattc cgggaactgg
    15661 gcgaaggccg agatcgccca accgcaaggc caactcctgc tgagccggtt ggagatgccc
    15721 tgcattcagc agtatatcga gtcatttccg aacatcacca ggcggcattt gggcttcagc
    15781 tattattcct atggtgccgg tgactgcctt ctgacccacg atgatacgga tcaaggtcat
    15841 ccggtcggtg ggaagcccgc tccccgtcgc cgtctcgcca tggtgagtta tttccatgaa
    15901 gaatgggaat gtgattgggg cggagagttg atgatctact cagcgaccgg cgaaccaacg
    15961 aacggaaagc cggaactggc cattacgcac tgcatcgcac ccaagccggg atcattggtc
    16021 atgtttaccg tgccgcggtt ccatcgggtg tgccgggtgg atcaaacggc gggagaccac
    16081 aagcgcctct cgattgccgg ctggttcatg accgaacatg cctgagcgtg tatcctattg
    16141 cagaacaatc gattgcagcg tgagcggaga ttgccgggtg gtgtgtttgc aagtcagcgg
    16201 aacacggcaa aatatttttc agaagttgag aagggcgagt ggttacaagt gccgatgggc
    16261 ggtcacgatg aggacttggg ccggttttcg gctcgtgttg cgccaacaat gatggatatc
    16321 agaaggaatg caggcggcat cccctcgttt gaggctgtac gtgttttctt ccaatgtcag
    16381 ttcgacggta ccctgaagca cgactgccaa ttgttccgcg cgccgtacat acggacgtga
    16441 tccgctggcc ccaccagact ccagtgtaat gaccatgggc tccagctgac tgtcttcgct
    16501 gattggtccg atcgcttcga ttctggcccg agaccattcg ctttccacta ccggccgggc
    16561 atcggcgcgg atgacagccg catgtgatgg actggtcgtt ctgaaaaatt ctccgagggt
    16621 tacgccgagc gccgatgcga tccgctcggt ggaggcaata gacggtgagg cctgccggag
    16681 ttcgacctgg gagataaaac taggggagaa gccgcatttg tccgctaggg ttcggacaga
    16741 gaggtgtcgg cttttgcgga gacggcggac gatgtcgccg acatgcgttt tcttccggtc
    16801 ctcggttgct acgctctcct gcggggcctt cttcttggat tttttctcca cgtcgtccta
    16861 tcctttgggt cctacccagg attcgcgcgg ttggtgtagc acgtttatgg gcctgtcgca
    16921 agccggccga aggggcggaa tcttctcaga catatctgcg gttgtcttcc aatagggaga
    16981 gtgttgtcac catgggacca ccctgctgtg tctctgcagc ctcttatgcc ggtagatact
    17041 tggttctcca ctccaacaac ggccgaacga ctcccggcca ccgaccgtga aagtctcctc
    17101 gcgccgaatc tccggtcccg ctgtcatcca cacgaaatcc aattgttcgg ggcgcatggg
    17161 cgtgcatgcg aaaaggatct tcggtcatca tcagggatga gaccgacatc attccttcgg
    17221 caagccagtt tcctggaatt ctggccgtgg aagtatatcg accggtcggg cgaggggttc
    17281 gctgccaggc ctggtcccgg tcatgggcga taaaaacaag gacatcttcc tcattgtaga
    17341 gattaaagac cggcacaaag atatgcccgg ctttcacgac atcaaattcc acttcgagat
    17401 caatcggttt tcgaatgtcg aagcggtccg tgatgatgcc ggactcagcc cggacgcgta
    17461 cggcacgcaa gcggacgacg tcattgcccg gagcctgggt tggatccagc cattcgacga
    17521 ccggctggat gtgtccggca tggaggtagt gattcaccac ttcatgcgcc ggccctgcct
    17581 gcacgatct
//
LOCUS       DAOQRM010000043        17454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2360_length_17454_cov_20.512903, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000043 DAOQRM010000000
VERSION     DAOQRM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17454
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2360_length_17454_cov_20.512903"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PKK81_05435"
     CDS             <1..433
                     /locus_tag="PKK81_05435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV32345.1"
                     /translation="LARQYLKGMLERGHGRIVFISSESGISPAPEMAHYSATKTMQLG
                     IARSLAELTRGTKVTVNSVLPGPTRTDGVEKFIQDVFPGLTQAEAECRFIAENRPTSL
                     IGRLIDPREIGDIVAFVCSARASVINGSCIRAEGGLVRTIC"
     gene            509..916
                     /locus_tag="PKK81_05440"
     CDS             509..916
                     /locus_tag="PKK81_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV32346.1"
                     /translation="MKGYVTDIEEATLKNSLYRQVLFTAKNSQLVLMSLKPGEEIGEE
                     VHELDQFLRFEAGDGTVILDGQDHAVRDGFAVVIPSGTRHNVINTSESADLKLYSLYS
                     PPEHKVGTVHRTKQEADADADHHFDGRTSIDSK"
     gene            complement(1164..2417)
                     /locus_tag="PKK81_05445"
     CDS             complement(1164..2417)
                     /locus_tag="PKK81_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV32347.1"
                     /translation="MSEEQSRQVRELVESVYRSDSRQVLATLIRLLGDFDTAEEALHD
                     AFAVAVEQWAREGVPANPRAWLVSTGRFKAIDGMRRRARFDASLTELARHLDLSTSDP
                     EGWNDEAVEDDRLRLIFTCCHPALSPETQVAMTLREVCGLTTDEIARAFLSKPATIAQ
                     RIVRAKGKIRDARIPYEVPSATDLPDRLDAVLRVVYLVFNEGYSASSGDSLTRHDLSG
                     EAIRLGRLLIGLLPEPEALGLLAVMLLHDSRRGARTSPTGDLVLLEQQDRSLWSRAQI
                     VEGTALVERALASRQVGPYTIQAAIAAVHAEAPDAASTDWAQIVGLYDLLVQAESSAV
                     VELNRAVAVAMRDGPEVGLVLVDAILSRGELVNYHLAHAARADLCRRLGRTAEACASY
                     EKALSLTQQEPERRFLKGRLSELAD"
     gene            complement(2478..2801)
                     /locus_tag="PKK81_05450"
     CDS             complement(2478..2801)
                     /locus_tag="PKK81_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNV32348.1"
                     /translation="MTTEHHGAASDPRVLEALRQVVDPELGINIVDLGLVYGSEVRDG
                     QVHVAMTMTTPACPMEELLMEMVHAAILRELPEARSVEVDLVWEPAWKPEMMSPSAKV
                     QLGRT"
     gene            complement(2850..3950)
                     /locus_tag="PKK81_05455"
     CDS             complement(2850..3950)
                     /locus_tag="PKK81_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32349.1"
                     /translation="MVVRHPQRLPLLALGMLALLTGLWAGLARLGWDVPLPRPGFSSL
                     HGPLMVSGFLGTLISLERAVALGRPWAYATPLLTGLGGVSLIVGAPLLAAQWLMLAGS
                     LGLVAIFAAIIRRHPALYTFTMGGGSLVWALGNLLWCLGWPVFLVVFWWAAFLVVTIA
                     GERLELARLQQVTGAAQATLLLLLGILLTGLLLLGWSFDGGVRLFGLGLAGLALWLGR
                     HDIARRTVKQAGLTRFIAICLLTGYVWLGISGLSAMWFGGVPVGPQYDATLHAFFLGF
                     VFAMIFAHAPIIFPAVLGARMTYRPLFYAHVVLLQVTLAVRLIGDAAGWSAGRQVGGL
                     LNAVTLLLFLVNTVSALQSPPERAGTAQGRGA"
     gene            complement(3969..4526)
                     /locus_tag="PKK81_05460"
     CDS             complement(3969..4526)
                     /locus_tag="PKK81_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV32350.1"
                     /translation="MSQEDSQAGPLAQFLAEDHRRLDALLQSAAAQPAQVNREVYDQF
                     RAGLLRHIGLEEKILFPAVQRWRGGTPLPIAAKLRLDHGALATLLMPTPTPAILATIR
                     RLLTDHNVLEEGPGGVYDLCDQLAGFEAESLLKDLRAAPPVAVMPHSDSPAVMTTLRR
                     TLERAGYRMEADMGRDVPVTPAGEP"
     gene            complement(4572..6017)
                     /locus_tag="PKK81_05465"
     CDS             complement(4572..6017)
                     /locus_tag="PKK81_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNV32351.1"
                     /translation="MRIVALLLIAVLLGACAIGPDYSRPDLAVPDRFRMAATQQETES
                     FANLPWWDLLQDEELRRLIRVALAENKDLKRAVASVEDFQARMLIAKMDFAPKADLTS
                     NAPLFGRKANFLFPGFPNPFNYYLQGNLSWEIDIWGRIRRSNEAARGDLLSREEARRA
                     VVLQLVSGVAEAYFDMLQFDMQLAVAQRTLKSWDESVRIAEARLREGMISKIDADQFA
                     AERANAAARAAELSRQMVQKENQLSVLLGRVPGQIPRGRSLTDQVMPPEVPAGLPSEL
                     LQRRPDLVQAEQELAAATARIGVAKADRFPKLSITGILGVASPQLSRLVANETAFGTA
                     GPGLAGPLLNAQILGFQQDAAEAQSREALARYEQALLVAFREVEDSLVAVRTVREQRN
                     AQVEQVDALRSALRLANLRYKGGLANYLDVLVAQRNLFEAELALTSTHRLQLVSVVQL
                     YKALGGGWSPLDTAQQQPGQAPAEPGKAGRG"
     gene            complement(6030..6341)
                     /locus_tag="PKK81_05470"
     CDS             complement(6030..6341)
                     /locus_tag="PKK81_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32352.1"
                     /translation="MLKITSEQTSDSARLTLEGSLSGPWVTELERVWQQVKQAGTFAP
                     VVELTGITFIAEEGRALLTSMWREGAVLVANGCCTRHIVQEITGAGGGQAASSVRCET
                     T"
     gene            complement(6383..9466)
                     /locus_tag="PKK81_05475"
     CDS             complement(6383..9466)
                     /locus_tag="PKK81_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV32353.1"
                     /translation="MRSFTDIFIKHPVLAVVVNLVIVLVGWRALTTLPVQQYPKIESS
                     SIIITTVYYGAAAETVRGFLTTPIERVVSAISGVDYLESTSRAGVSTVTVHLKLNHNS
                     TAALAEVTARLQQVRSELPAEAEPPAVEVQRADRPYASFYLSFTSPERTVPALTDWLL
                     RTLQPQLATLTGVQRVTIEGGRQIAMRVWIDPDRLAAYNLSPGDVQAALRRNNYLAAV
                     GRTKGNLVQVNLLANTDLRSVNEFQELIVADRGGAIVRLRDVARVEKGAEEAEMVAKY
                     NRLEGVYLGVWPLVGSNEIEVAHRLREEMDRIRPTLPKDIDMQLVWDGTMFMESALTE
                     ITKTLGETILIVATVVFLFMGSIRTALVPLVAMPVSLIGAAIFMYAFGFSLNLLTILA
                     IVLSVGLVVDDAIVVVENVERHVREGKSRIQAALIGSRELVGPIIAMTITLAAVYAPI
                     GFQGGLTGSLFLEFAITLAAAVVLSGVVAITLSPVMSSRFVHPEGKEGKLTRLVNRGF
                     DATRAVYGRLLDGALEMRWAIVVASLLIMVAAVPLYLFSRQELAPVEDQSHISLFLEA
                     SPDSTVAATNSDSLKVVEAIRAFPEARFMWSLTSSWGGFGGLVAKDWRERTRSTELMY
                     GEVFGAVSQIPGLRVFPRLDPPLPTPGQYDVELILASEVPAEQLLEMTAAVLGAGWQS
                     GKFLYVDTDLKIDLPEARVVLDRERLADLGFDLAGVGQELGTLLGGAYVNRFNFYDRS
                     YKVIPQIGDKDRATLDPLLDLKIKTPSGQLVPVSTFTHIETSTAPRTLNRFQQRNAVR
                     IFGGVKPGVTKEEGLRVLETAAASAVGPRVALDFAGESRQIRHEGSALTVTLGFAVVL
                     IYLVLAAQFKSFRDPLIVLLGSVPLAISGALVFSFLDLTTINIYSQVGLITLVGLIAK
                     NGILIVEFANTLQERGLSKTAALREASLTRLRPVLMTSAATVFGHLPLVLVSGPGAAA
                     RNSIGMVLVTGMTVGTIFTLFVVPVFYSLIAARHQPAVELDAAEEGELQLAAAKG"
     gene            complement(9484..10608)
                     /locus_tag="PKK81_05480"
     CDS             complement(9484..10608)
                     /locus_tag="PKK81_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV32354.1"
                     /translation="MNRRSWIGSSLLLLTVAAAGVGLAAWKYGNIQANAAASANQPEP
                     MESVSVSVARDVEHHQTATSIGTVLALRSITLRNELAGTVSQVKFVPGQTVEAGAVLV
                     ALDVSVEEADLRAQQAQAVLAKTVLDRRRALTQDQAAAQEEVDRARADLDVAHAQMAR
                     TKALIARKTIRAPFRARVGLADVHPGQYLNEGTQLTTLQGIDEAVHVDFAVPQHVALG
                     LRQGATVEVFTGDSSPVAARIVAIDARIDPATRNTMVRARVEHSATMPAPGAAVRIRV
                     PVGLARKAVAVPVNALKKGPGGDQVFVIEPDRENKQRAHVRQVESGTMLGDDIVIYSG
                     LNAGERVAASGAFKLRDGVLVVAAGEPARTPEPDHLLSQQ"
     gene            complement(10890..>11766)
                     /locus_tag="PKK81_05485"
     CDS             complement(10890..>11766)
                     /locus_tag="PKK81_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32355.1"
                     /translation="AVQRVFGTHHQTEELEPITPEELAVALPDPQIRRQLVNGLVVMS
                     LIDREVTPQEADLVERFAAALQVTAPEVTNLRHVVKRELFHLRLDLARRFWLREKVAE
                     IWKQEGLRGLAKFAAGMIGRYEDATVAARYQALEEYPAGSLGRSYWEYCRKNGFALPG
                     EKGGAPEAILFHDCAHILSGYGTAPEEEVQVACFSAGFQRRDPFLFVFFVLLQFHVGI
                     RMTPITAARTGFFDPERALIAMRRGAAMNVDLNHGWDYWPVMGEQVEELRHRYNILPI
                     EAFRTRTESESEQVR"
     assembly_gap    11767..11866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11867..12741)
                     /locus_tag="PKK81_05490"
     CDS             complement(<11867..12741)
                     /locus_tag="PKK81_05490"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNV32356.1"
                     /translation="MESLTNMLAQHGALVLFAVVFAEQVGLPFPALPLLVAGGVLVGT
                     GHLAWSSALGAAILATLMADAIWYLAGQWRGRPVLSLLCLIALEPDACVRRTEDFFLR
                     HGPKSLIVGKFIPGLSTIAPPLAGIVGLSVPLFLLYDTLGALLWAGSGLEFGYAFSGQ
                     IEQAFAYSEQVMPALLLTMVFLLAGFVSLKAWRRHRQLRLVPRMTVAELAEKLTAAEP
                     PVLIDVRPRARAEAEPGIPTAVLMSLDELARRYQEFPRHRDVVVYCGCPADVASAQGA
                     LLLQKKGFTRVWPLAG"
     gene            complement(13315..>15855)
                     /locus_tag="PKK81_05495"
     CDS             complement(13315..>15855)
                     /locus_tag="PKK81_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV32357.1"
                     /translation="LQVAEAISLHRDLPALFRDLAQRLPAVAPFDFIGLVLHDPAKQV
                     MKVHVLETVEARGVTNRLDGMEIPMEESASGWVWSHQQPLMIPSLADETRYKPGLGAL
                     RSIGVQSLCFCPLTTAMRRLGAIGFGSLRTSAFGDADVEFLNQVAKLVAVAVDNVLHH
                     QALSDERDRLQLLLDVTESIASHRDLTRLLEDLAGRLPQLVPFDFINAVLHDPATDKM
                     RLWLLVTSEPSTLRPGLETPIEESPGGLVWKTQQPLTVHDVAQEDRFPGLMTLFRENG
                     VRAFCVVPLTTAHRRLGAMGFGSLQPRVYEKREIALMQQVAKQVAVALDNALNGETAL
                     TYQGQLTRERDRQRLLLEVNNAVVSHLDLDQLFPAVSACLRRVIQHDGSSLLLCNDET
                     GQWRIHVLDFDRNESFIEEGRIEESPRSPSCQAITTGKAAVFGEDELREMAESSPIAQ
                     ELLDFGAKSFCSLPLLSHNRTLGALNVCRRGEGGFSPEDVQLLAQVAQQVAIAVENAL
                     AFREIAALKDKLAKEKVYLEEEIQTAYNFEEIVGDSRALKQVLKQVQTVATTGSTVLI
                     LGETGSGKELIARALHNLSDRRERTFVKLNCAAIPTGLLESELFGHEKGAFTGAIATK
                     IGRFELADGGTIFLDEVGEIPLELQVKLLRVLQEQEFERLGSTRTIRVNVRVIAATNR
                     DLGQMVEEQRFRSDLYYRLKVFPMTVPPLRERAEDVPLLVRHFVQKFAARMKRRIETV
                     PAEAMKALQGYSWPGNVRELENYIERAVILSSGSELFVSTAELKRAAPVGNGSATTLE
                     DAEREHILKALRETNWVIGGDAGAAARLGMKRTTLQSKMQKLGIARPR"
     assembly_gap    15856..15955
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16396..16944
                     /locus_tag="PKK81_05500"
     CDS             16396..16944
                     /locus_tag="PKK81_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNV32358.1"
                     /translation="MGWIVVVALVLLVMIVIGMYNSLVRLRAACDSAWADIDVQLKRR
                     YDLIPNLVETVKAYAAHEKGALEAVINARAKAMSAQGPEAKGTAETQLTQSLKSLFAL
                     AEAYPQLRAVEAFNQLQGSLNQIEDAVQNARRYYNAVVRDYNAKTQEIPTNLIAGWFN
                     FVGRQFFELTDTTQREPPKVQF"
     gene            16946..>17454
                     /locus_tag="PKK81_05505"
     CDS             16946..>17454
                     /locus_tag="PKK81_05505"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNV32359.1"
                     /translation="MGRPAGPHVSAGRSPRLAPRLLIVPLLCFLTFSQLLPAAARSFV
                     LSRFDVELQVLSGGDLLVTETVSPRFEGAWNGIERLIPVEYRTPQGFNYSLLLDRVTV
                     TDEQGTPLTFESSRERHYRNLKIWIPGATDATRTFVLKYRVRNGLKFFEDHDELYWNV
                     TGDEWDVPI"
BASE COUNT         3520 a         5781 c         4940 g         3013 t
ORIGIN      
        1 cctggctcga cagtatctca aggggatgct ggaacgcggc catgggagga ttgtattcat
       61 ttcgagcgaa tcggggattt cccccgcgcc ggagatggct cactacagcg ccaccaagac
      121 gatgcaattg ggaatcgccc gctctcttgc tgagctcacc agaggcacga aggtcacggt
      181 gaattcagtc ctgcccggcc ccacgcgcac ggatggcgtt gaaaaattca tccaggatgt
      241 ctttcctggc cttactcagg cggaggctga atgtcgattc attgcggaga accgtccgac
      301 gtcactcatc ggtcgcctca tcgacccacg ggaaatcgga gacatcgtcg ccttcgtgtg
      361 cagtgcgcgt gcttcggtca tcaacggatc ctgcatccgc gccgaaggcg gtcttgtgcg
      421 caccatatgc tgaggttata gacaagccag cgacctgtac cctatctcgt ccccactgcc
      481 acagaggctt taacgaaagg tggtcaacat gaaggggtat gtcacggaca tcgaagaggc
      541 gaccctgaag aattcactct accggcaagt cctgttcacg gccaagaata gtcagttagt
      601 gctgatgagc ttgaaaccgg gagaggagat cggcgaagaa gtccatgaac tcgatcaatt
      661 cctccggttt gaagcagggg acggaacagt catactggat gggcaggacc acgccgtgcg
      721 cgacgggttc gccgtcgtca tcccgtccgg aaccagacat aatgtcatca atacgtcaga
      781 aagcgccgac ctcaagctgt atagtcttta tagtccaccg gagcacaagg tcggcaccgt
      841 ccaccgaacc aagcaggaag cggatgccga tgccgaccac cattttgacg gtcggacatc
      901 catagacagc aaataagccc atccggctgg cgaagcctgg gtcaacctgt ccgatgactt
      961 cgccacgtta tcgactttcg gtctggacct tcgcggccat cgacgcgacg cgcgcgacat
     1021 cagaactcat ggctacgtcg ggtgacgtca acgtctcgat tgcgaaccaa tccgcctcca
     1081 aggcatcgtc ggccgcctgt ggcacactcg acatccactg gcataacacg gcaattcaca
     1141 cgtcgtgcga ggaagcgcct tgctcagtcg gccaactcac tcaagcgtcc cttcagaaat
     1201 ctccgttccg gttcctgctg cgtgagactc aaagccttct cgtacgaggc gcaggcttct
     1261 gcggttcgcc ccagtcgccg gcagaggtcg gcgcgggctg catgcgccag gtgatagttc
     1321 acgagttcac cgcgggagag aatcgcatcg accaagacca gccccacctc cgggccgtcg
     1381 cgcatcgcca cagccaccgc ccggttcagc tcgaccacgg ctgacgactc agcctgtacc
     1441 agcaaatcgt agagaccgac gatctgtgcc cagtcggtcg atgcggcatc gggggcctcg
     1501 gcatgcacgg ccgcaatcgc ggcctgaatg gtatagggac cgacctgacg cgatgcgagc
     1561 gcccgctcta ccaaggccgt tccttcgaca atctgcgccc ggctccacaa cgagcggtcc
     1621 tgctgctcca ggagaaccag atcgccggtc ggcgaggtgc gcgccccgcg acgcgagtcg
     1681 tgcagcaaca tcaccgccag gaggcccagg gcttcgggct ccggcagtaa cccgatcaac
     1741 aggcgcccca ggcgaatcgc ctccccggac agatcgtgcc gcgtgagtga gtcccccgac
     1801 gaagcgctgt agccctcatt aaagacgagg tacacgaccc gcaacaccgc atccagccgg
     1861 tcaggcaaat cggtcgccga cggcacctcg tagggaatgc gggcgtcacg gatcttgcct
     1921 ttggcccgca cgatgcgttg cgcgatggtg gcaggcttgc tgagaaatgc gcgcgcgatc
     1981 tcatcggtcg tgaggccgca gacttcgcgc aacgtcatcg caacctgcgt ctcgggcgat
     2041 aaggccggat ggcagcaggt gaagatcagt cgcagccggt cgtcctcgac cgcctcgtcg
     2101 ttccagcctt cgggatcgct cgtgctgagg tccaggtgtc tcgcgagttc cgtcagggat
     2161 gcgtcgaagc gggcgcgccg tcgcatcccg tcgatggcct tgaagcggcc ggtcgagacg
     2221 agccaagccc ttggattggc cggcaccccc tcccgtgccc actgctccac agcaacggca
     2281 aaggcatcgt gcagggcctc ttccgccgta tcgaagtccc cgagcagacg aatcaaggtg
     2341 gcgagcacct ggcgggagtc cgatcgatac accgactcca ccaactcgcg cacctgcctg
     2401 gattgctcct cactcacggt ccgggtgcct ctcagccaag caaacttaaa cagtctttcg
     2461 cgtccactcg ctcatcttca cgtccggccc aactgcacct tggcagacgg actcatcatc
     2521 tccggcttcc aggcgggctc ccacacgaga tccacttcca ctgaacgcgc ctccggcaac
     2581 tcacgcagaa tcgccgcatg caccatctcc atcaacagct cttccatcgg gcaggcgggc
     2641 gtcgtcatcg tcatggccac atgaacctga ccgtcccgca cctcgctgcc gtataccaat
     2701 ccgagatcca cgatgttgat acctaactcg ggatcaacca cctgccgcag ggcctcgagc
     2761 actcgtggat cggacgctgc accgtgatgc tccgtcgtca ttcaactggt cctttcaccg
     2821 gcgaagccgc cgtctgcagt tcgcacacat cacgcccccc ggccttgcgc tgtccctgct
     2881 cgctccgggg gactctgtag cgccgacact gtgttgacga gaaacaggag cagcgtcaca
     2941 gcattgagca ggccgccgac ctgacgaccg gcgctccacc ctgccgcatc accgatgaga
     3001 cgcacggcca gagtgacttg caacaggacc acatgcgcat agaacagcgg ccgataggtc
     3061 atccgtgcgc cgagtactgc cgggaaaata atcggcgcat gggcgaagat catcgcgaag
     3121 acaaacccga gaaaaaaggc atgcagcgtg gcgtcgtact gcggacctac gggaacaccg
     3181 ccgaaccaca tggcagacaa cccgctgatg ccgagccaga catacccggt gagcagacag
     3241 atcgcaataa accgcgtcag ccctgcttgc ttgacggttc gcctggcaat gtcatgccgc
     3301 cccaaccaca gggctagacc ggccagcccc aacccgaaca gacgaacgcc cccgtcaaac
     3361 gaccagccca gcagcaacag ccccgtgagc agaatgccga gcaacagcag aagcgtcgcc
     3421 tgcgcggctc cggtcacttg ctgcagccgg gccagttcca ggcgctcacc ggcgatggtc
     3481 accacgagaa acgccgccca ccagaacacg accagaaaga cgggccaccc gagacaccag
     3541 agcagattgc ccaacgccca gactagcgat ccgcccccca tggtgaacgt gtacagcgcc
     3601 gggtgtcggc gaatgatcgc cgcaaaaatc gcaaccagcc cgaggctgcc ggcaagcatc
     3661 aaccattgcg ccgcaagaag cggggccccg acgatcaagc tcacaccgcc gagtccggtc
     3721 agcagcggcg tggcataggc ccagggccgg cccagagcca ccgcacgctc caagctgatc
     3781 agcgtaccca gaaagccgga caccatgagc ggcccatgca aggaggaaaa tccaggtcgc
     3841 ggaagcggca catcccaccc gagccgcgca agccccgccc agagtccggt gagcaacgcg
     3901 agcatcccca aagccaggag cggaagacgt tgaggatgtc ggacaaccat agacgcgtac
     3961 actttccgtc agggttcccc ggccggagtc accggcacat ctctacccat gtccgcctcc
     4021 atgcgataac cggcccgctc caatgtgcgg cgtagcgtgg tcatcaccgc agggctgtcg
     4081 gaatggggca tcacggcaac cggcggagcg gcgcgcagat ccttcagcag tgactcggct
     4141 tcaaagcctg ccagctgatc gcagagatcg tagacaccgc ccggtccctc ttccaacaca
     4201 ttgtggtccg tgagcaggcg ccggatggtc gccaggatcg ctggggtggg agtaggcatg
     4261 agcaacgtgg ccagcgcgcc gtgatcgaga cgtagcttgg cagcgatcgg aagcggtgtg
     4321 ccgcctcgcc accgttggac ggcagggaac agaatcttct cctccaagcc gatgtgccgg
     4381 agcagtccgg cacgaaattg atcgtagacc tcgcgattga cctgggccgg ttgggccgcc
     4441 gccgactgca acaatgcgtc gagccgtcga tggtcttcgg cgaggaattg cgcgagaggg
     4501 cctgcctgac tgtcctcctg gctcatgccc cggctcccgt catatcgccc tctttccccc
     4561 ggatccgtgg gtcagccgcg ccccgccttt ccgggttctg ccggagcttg gcccggctgt
     4621 tgctgggccg tatcgagcgg cgaccatccg ccccccagcg ctttatacaa ttgcacgacc
     4681 gacaccagct gtaaccggtg ggtgctggtt aacgccagtt ccgcctcgaa cagattgcgt
     4741 tgcgccacga ggacgtcgag atagttcgcg agtcctcctt tataccgcag attggccaga
     4801 cgcagagccg aacgcaacgc gtccacctgc tcaacctgcg cattccgctg ctcccgcacc
     4861 gttcgcacgg caaccagcga atcctccact tcacgaaacg ccaccagcaa cgcctgctca
     4921 taccgtgcca gcgcttcacg gctctgcgct tcagccgcgt cctgctgaaa accgaggatc
     4981 tgagcattca gcaagggacc ggcaagcccg ggacctgcgg taccgaacgc cgtctcattc
     5041 gccaccaagc gggacaactg cgggctggcg acaccgagaa ttcccgtgat gctgagtttc
     5101 ggaaaccggt ccgccttcgc cacaccgatt cgcgccgtgg ccgcagccaa ttcctgttct
     5161 gcctgcacga gatccgggcg gcgctgcagc aattccgaag gaaggccggc cggcacctcc
     5221 ggcggcatca cctggtcggt gagggaacgt cctctcggaa tctgacccgg cacacgcccc
     5281 agcaacacac tgagctggtt ttctttctgc accatttgcc ggctcaactc tgccgcccgt
     5341 gctgcggcgt tggcgcgttc cgccgcgaac tggtccgcat cgattttgga gatcatgccc
     5401 tcccgcagcc gggcctcggc aatcctgacc gattcgtccc aggatttcag cgttcgctgc
     5461 gcgacggcca actgcatgtc gaactgcagc atgtcgaagt aggcctccgc cacgccgctc
     5521 accagttgca agacaaccgc gcggcgagcc tcctcccgcg acaggagatc tccccttgcc
     5581 gcttcattcg atcgacgaat gcggccccag atatcgatct cccaggagag attcccctgg
     5641 agatagtagt tgaacggatt gggaaagccc ggaaacagaa agtttgcctt gcggccgaac
     5701 aagggcgcgt tggacgtgag atcggccttc ggcgcgaaat ccatttttgc gatcaacatg
     5761 cgggcctgaa aatcttcgac cgacgcaacc gcccgtttca gatctttatt ctcagccagc
     5821 gccactcgaa taagacgccg cagttcttca tcctgcaaca gatcccacca gggcaggttg
     5881 gcaaacgact cggtttcctg ctgggtcgcc gccatgcgga accggtccgg cacagcaagg
     5941 tcgggacggg agtagtccgg cccgatggca caggcgccca ggagcacagc gatgaggagg
     6001 agagcgacaa tgcgcatgcg atgatcgtcc tatgtcgtct cacagcggac ggatgaggca
     6061 gcctggccgc ctcccgcccc ggtgatttct tgcacgatat gtctggtgca gcacccgttc
     6121 gcgaccagga cagcaccctc ccgccacatg ctggtcagca gagccctgcc ctcctcggca
     6181 atgaacgtga ttcctgtcag ttccaccacg ggggcaaacg tgccggcttg tttgacctgc
     6241 tgccacaccc gttcgagttc agtcacccag ggtccgctca gactcccttc caaggtcaga
     6301 cgcgcggaat cggaagtctg ctcggacgta atcttcagca tcgtgcatgt cctcctgttg
     6361 cgctcaacga gccgggatca gttcatccct ttgccgccgc caactgcaac tcaccctctt
     6421 cggccgcgtc cagttctacc gccggctgat gtcgggccgc gatcagcgag taaaacaccg
     6481 gcacgacgaa cagcgtgaag atggttccga ccgtcatccc cgtgaccaac accatgccga
     6541 tgctgttgcg ggccgcggcg ccgggtcccg aaacgagcac cagcgggagg tgcccgaaga
     6601 ccgtggcggc cgaggtcatc aggaccgggc gcaatcgcgt cagggatgct tcgcgcaggg
     6661 cggccgtctt cgacagccct cgttcctgca gagtattcgc gaactccacg atcaaaatgc
     6721 cgttcttcgc aatcagcccc accagtgtaa tcaacccgac ctgcgaatag atgttgatcg
     6781 tcgtcaggtc gaggaaactg aacaccagcg cgcctgagat cgccagcggc accgagccca
     6841 gcaggacgat cagcgggtcc cgaaaacttt tgaactgcgc cgcgagcacg aggtaaatca
     6901 gcaccaccgc aaagcccagt gtcaccgtca aggccgaacc ttcgtggcgg atctggcgcg
     6961 attctcccgc aaaatcgagc gccacccgtg gaccgacggc cgaggccgcc gcagtttcca
     7021 gaacgcgcag cccttcctcc ttcgtcaccc cgggtttcac cccgccgaag atccgcaccg
     7081 cattacgttg ttggaaacgg ttcagcgtcc gcggcgcagt actcgtctca atatgcgtaa
     7141 acgtcgagac cggcaccagc tggccgcttg gtgtcttgat cttgagatcg agcagcggat
     7201 ccaacgtcgc gcgatccttg tcgccgattt gaggaatgac cttgtaactg cggtcgtaaa
     7261 aattgaaccg attcacatag gccccgccga gcagcgtccc cagttcctga ccgaccccgg
     7321 ccagatcgaa gcccaggtcg gcgagtcgct cgcgatcgag cacgacccgc gcttcaggca
     7381 aatcaatctt caggtcggtg tcgacataca ggaacttgcc gctctgccaa ccggcaccca
     7441 ggaccgcggc ggtcatctcc aacagttgct cggcaggcac ttcactcgcc aggatcagtt
     7501 ccacatcata ttgcccgggc gtcggcaatg gcggatcaag gcgcgggaac acccgcagac
     7561 cggggatctg cgacacggct ccgaagactt cgccgtacat cagctcggtc gagcgcgtcc
     7621 gctcccgcca gtccttggcc accaggccgc cgaagccgcc ccatgacgag gtcaacgacc
     7681 acatgaagcg ggcttccgga aacgcccgga tcgcttcgac gaccttcaag gaatcactgt
     7741 tcgtagcagc caccgtcgag tccggcgacg cctccagaaa gagactgata tgactctggt
     7801 cctccacagg cgccaattcc tgccgtgaaa agagatacag cggaacggcc gcaaccatga
     7861 tcagcaacga ggccacgacg atcgcccagc gcatctccaa cgcgccgtcg agcaaccgcc
     7921 cgtagaccgc ccgcgttgca tcgaatccac ggttgaccag acgggtcaac ttgccctcct
     7981 tgccctccgg gtggacgaac cgcgagctca tgaccggcga gagcgtgatc gccacgacgc
     8041 cggacagcac gactgcggcg gccagggtga tggcaaactc cagaaacagc gagccggtca
     8101 gacctccctg aaaaccgatc ggagcataga ctgcggccag ggtaatggtc atggcgatga
     8161 tgggcccgac caattcgcgc gagccgatca acgccgcttg aatccgcgac tttccttcgc
     8221 gcacatgccg ttcgacgttc tccacgacaa cgatcgcatc gtccaccacc aatcccaccg
     8281 acaacacgat cgccagaatc gtcaacagat tcaggctgaa gccgaacgca tacatgaaga
     8341 tggcggctcc gatcagcgac accggcatgg cgaccagcgg gaccagtgcg gttctgatcg
     8401 atcccatgaa caggaacacc accgtggcca cgatcagaat cgtttcgccc agcgtcttgg
     8461 taatctccgt caaggcgctt tccatgaaca tggtgccgtc ccagaccaac tgcatgtcga
     8521 tatctttcgg cagggtcggc cggatacgat ccatttcttc ccgcaaccgg tgggccactt
     8581 caatctcgtt ggagccgacc agcggccaga ccccgaggta caccccctcc aggcggttgt
     8641 acttcgccac catctccgct tcttcagcgc ctttttccac tcgcgccaca tccctgagcc
     8701 gcacgatggc gccgccacgg tccgccacga tgagttcctg aaactcgttc acggaacgca
     8761 ggtccgtatt tgcgagcaga ttgacctgca cgaggttgcc cttggtccgg ccgacggcgg
     8821 ccaggtaatt gttgcgccgc aacgcggcct gcacatcgcc gggagagagg ttgtaggccg
     8881 caaggcgatc gggatcgatc cacacgcgca tagcgatctg ccgtccacct tcaatcgtga
     8941 cgcgttgcac cccggtcagg gtggccagtt gcggctgcag ggttcgcaac agccaatccg
     9001 tcagcgccgg cacggtccgc tccggagacg tgaaactgag ataaaacgac gcataggggc
     9061 ggtcggcacg ctgcacttcc accgccggag gttccgcctc agccggcagc tccgaccgga
     9121 cctgttgcaa ccgcgccgtc acttcagcga gtgccgccgt gctgttgtgg ttcagcttca
     9181 aatgcaccgt cacggtgctg acgccggcgc gactggtgga ttcgagataa tccaccccgc
     9241 tgatcgccga cacgactcgc tcgatcggcg tggtgagaaa tccccgcacc gtctctgccg
     9301 cggctccgta atagaccgtc gtgatgatga tcgaggagct ctcgatcttc ggatactgtt
     9361 gcaccggcag cgtcgtcagc gcccgccagc ccacgaggac gatcacgagg ttcacgacca
     9421 cggccagcac ggggtgcttg atgaagatgt cggtaaatga acgcatgggc ctgctcccct
     9481 gacttactgt tggctgagaa ggtgatccgg ttccggtgta cgcgccggct cgccggcggc
     9541 gacgacgagc acgccgtcac gcaacttgaa cgcccccgac gcggccaccc gctcgccggc
     9601 gttcagaccg ctatagatga cgatgtcgtc accgagcatc gtcccgcttt cgacctggcg
     9661 cacatgcgca cgctgtttat tctcgcggtc cggctcgatg acaaacacct gatcgccacc
     9721 cggacccttt ttcaacgcat tcaccggaac cgccacagcc ttgcgcgcca accccaccgg
     9781 caccctgatc cgcacggccg cgccgggagc cggcatggtg gcggaatgct cgaccctggc
     9841 acgaaccatg gtgttgcgcg tggcggggtc gatgcgggca tcgatcgcca caatcctggc
     9901 ggccaccgga gaggagtcac cggtgaagac ctcgacggtc gccccttgcc gcaatccgag
     9961 cgcgacgtgc tgcggaaccg caaagtccac atggaccgcc tcatcgatgc cctgcagcgt
    10021 cgtcagttgc gtgccttcgt tgagatattg cccgggatgc acgtcggcca atcccacacg
    10081 ggcgcggaac ggggcacgga tcgtcttgcg ggcgatcaag gccttcgtgc gcgccatctg
    10141 ggcatgcgcg acatcgaggt ctgcgcgggc ccgatcgact tcctcctgag ccgcggcttg
    10201 gtcttgggtc aacgcccgcc gacgatccag cacggtcttc gccaggaccg cctgggcctg
    10261 ttgggcccgc aagtccgctt cttccaccga cacatcgagc gccacgagca cggccccggc
    10321 ctcgaccgtc tggccgggca caaacttgac ctggctgacg gtgccggcca gttcgttccg
    10381 cagcgtgatc gagcgcaacg ccagcacggt gccgatagaa gtggccgtct gatgatgctc
    10441 gacatccctg gcgacagaga cagacaccga ttccatcggt tcgggctggt tcgccgaggc
    10501 cgccgcgttc gcctggatgt tgccatactt ccacgcagcc aggcccacac cggccgccgc
    10561 cacggtcagg agcagcaacg aagacccgat ccaacttcga cgattcatga cagttgccct
    10621 cccggcaggt tgcagatgga cctgagactc tcccgttcca tcacaagaat tcgttcctct
    10681 gcaaatagca acgactgttc ctgtctcgcc atcggacgca ttccaatcta agtgcgcatt
    10741 cgaatggaga aaatattctc ccgtccgccc gtgcttcgtt catggctgag gtggatgacg
    10801 aagtccgcct gcctgccatt cggcatctgc cgaaataacg gcagcgtcca accgactcac
    10861 aaacttgcag acagagggga cgtctgatgt cagcgtacct gctccgattc actctcagtc
    10921 cttgtgcgga acgcctcgat aggcagaatg ttgtatcgat gccgtagctc ctcaacctgt
    10981 tcacccatca ccggccaata gtcccagcca tggttgagat ccacattcat cgccgcgccg
    11041 cgacgcatgg cgatcaaggc cctctccgga tcgaagaacc cggttctggc cgcagtaatc
    11101 ggcgtcatac ggataccgac gtgaaactgg agcagcacaa aaaacacgaa gaggaacgga
    11161 tcacgccgtt gaaagcctgc actgaagcag gccacttgaa cttcctcctc gggcgccgtg
    11221 ccgtagccgg acaagatgtg ggcacaatcg tgaaacagaa tggcctcggg tgcaccacct
    11281 ttctctcccg gaagcgcgaa gccgttcttc cggcaatact cccaataact ccgccccaac
    11341 gaaccggccg gatactcctc caaggcctgg taccgagctg cgacggtcgc atcttcatac
    11401 ctgccgatca tgccggcagc gaattttgcg agcccccgca gtccttcctg cttccaaatc
    11461 tcggccacct tttccctgag ccagaagcgc cgggccaggt cgagacgcaa atgaaacaac
    11521 tctcgcttga cgacatgacg aagattcgtg acctcgggcg ccgtgacctg gagggccgca
    11581 gcaaaccgtt cgacgagatc ggcctcttgc ggcgtgacct cgcgatcgat cagcgacatc
    11641 acgaccagcc cgttgaccaa ctgacgtctg atttgcggat cgggaagcgc cacggccaac
    11701 tcttccggag taatcggttc cagttcttcc gtctgatgat gcgtgccgaa cacccgctgg
    11761 accgctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgg ccagcggcca
    11881 cacgcgagtg aaccccttct tctgtaaaag gagcgcacct tgagcgctgg ccacatccgc
    11941 cggacacccg cagtagacga ccacgtcgcg atggcgcggg aattcctggt aacgccgggc
    12001 caattcatcc aacgacatca gcaccgccgt gggaatgccc ggttccgcct ccgccctcgc
    12061 gcgcggtcgg acatcgatca gcaccggcgg ttcagccgcc gtcagcttct ccgcgagctc
    12121 tgccaccgtc atgcgcggca ccaggcgaag ctgccggtgc cgacgccagg ctttaaggga
    12181 tacgaagccg gccagcagaa acaccattgt gagcagcaag gcaggcatca cctgctccga
    12241 ataggcgaag gcctgctcga tctgaccgct gaacgcatag ccaaactcca atccagatcc
    12301 cgcccataac aacgcaccga gcgtgtcata gagcaggaac aggggcacac tcagcccgac
    12361 gatgccggcg agcggagggg cgatcgtact caacccggga atgaatttcc ccacgatcaa
    12421 cgacttcggt ccgtgccgca gaaaaaagtc ctccgtgcgc cggacgcagg catccggttc
    12481 caacgcgatc aggcagagca gactgaggac gggacgtccg cgccattgtc ccgccaggta
    12541 ccagatcgcg tccgccatca gcgtggcgag gatcgccgcg ccgagcgcac tgctccaggc
    12601 cagatggccg gtgccgacca acacccctcc cgccaccagg agcggcagcg ccgggaaggg
    12661 cagtccgact tgctcggcaa acacaacggc gaacaggacg agtgctccgt gctgagccaa
    12721 catgttcgtt aaggattcca tcacgtcacc ccaatctccc tctctcgaca accgtgcttc
    12781 tcgtcatgac catacccgcg gcttacctcc agaccggcaa ggcagcaagc cgcctggttt
    12841 gcacccgggg aacaacggca tggcgaattg gatagggccg agccgtccca tcccgcgaga
    12901 ggagatggag ttcctcaatg aggttgccgc aggcggcgca agtccaagcg gtgattgcca
    12961 gtgatccact gtcgtcgacc acggtgcgaa ccggtcccgc gactcgatcg gcctgtccgt
    13021 ggcggcagtt cgcccgactc gacgttgcag cagtagccat ggagacgaac cgtcggttct
    13081 ttatggatga catgggacgc tcctctcatc gcattgaaat gaagactgcg gtcatgctcg
    13141 gatatcagtc ggagaaagca tggaccgttc cagcgatgtg gaactcgtga cttgcagaga
    13201 gactgcttgc aggacgggga gttacgcagt gaggagcgac ggtgtcgaag acaatgagaa
    13261 tgcggtgccg aaggagtgac tgccgagatg tcggcagctg tcgaaagatc ggcgctaccg
    13321 cgggcgcgcg atgccgagct tctgcatttt agactggaga gtggttcgct tcattcccaa
    13381 acgggccgca gccccggcgt cgccgccgat aacccagttc gtctctctca gcgccttgag
    13441 aatgtgctcg cgctcggcgt cctccagcgt cgtggccgat ccgttcccca ccggggcagc
    13501 gcgtttcagt tccgcggtgg agacgaacag ctccgagccg gaggacagga tcaccgcccg
    13561 ttcgatgtaa ttttccagct cccgcacatt gccgggccag gaatagccct ggagcgcttt
    13621 catggcctca gccggaacgg tttcgatccg ccgcttcatg cgcgcggcga acttctggac
    13681 gaaatggcga accagcaagg gcacatcctc cgcccgctcg cgcagaggcg gcacggtcat
    13741 cgggaacact ttcagccgat agtagagatc gctgcgaaac ctctgctcct ccaccatctg
    13801 gccaagatcc cgattcgtcg cggcgatcac ccggacattg acacgaatgg ttctggtgct
    13861 gcccaaccgc tcgaactcct gctcctgcaa cacccgcaag agcttcacct gaagttccaa
    13921 cggtatctcc cccacttcat ccaggaagat ggtcccgccg tcggctaact cgaatcgccc
    13981 gatcttcgtc gcgatggccc cggtaaaggc tcccttttcg tgaccgaaca gctcgctctc
    14041 cagcagcccg gtgggaatcg cggcacaatt gagtttcacg aaggtccgct cccttcgatc
    14101 gctgagattg tgcaaggccc tcgcgatcaa ctccttcccg ctgcccgttt caccgagtat
    14161 cagcacggta gaaccggtgg tcgcgacggt ctgaacctgt ttgagcacct gcttcagggc
    14221 gcgactgtcg ccgacgatct cctcgaagtt gtacgccgtc tgaatttctt cctcgagata
    14281 gaccttttct ttggcgagct tatccttgag cgcggcaatc tcccggaacg cgagcgcgtt
    14341 ctccaccgca atcgcaactt gctgcgcgac ctgggccagg agctgcacgt cctccgggct
    14401 gaagcctccc tcaccgcgcc gacagacgtt caaggctccg agggtgcgat tgtgcgacag
    14461 gagcggtaag gagcagaacg acttggcgcc aaagtccagg agctcctgtg cgatgggtga
    14521 agactctgcc atctccctga gctcgtcttc accgaacacg gccgctttgc cggtcgtgat
    14581 cgcctggcac gagggagagc gtgggctctc ttcgatccgt ccttcttcga taaagctttc
    14641 gtttcggtcg aaatccagaa cgtggatgcg ccattgccct gtctcgtcat tgcagaggag
    14701 caggctggag ccatcgtgtt gaatcacccg acgcagacag gcgctgaccg ctggaaacag
    14761 ttgatccagg tccaggtggg acaccaccgc attgttgacc tcgagcaaca ggcgctggcg
    14821 atcgcgttcc cgcgtcagct gcccctgata ggtcaaggcc gtttcgccgt tgagcgcatt
    14881 gtccagggcc accgccacct gcttggccac ctgctgcatg agggcgatct cacgtttctc
    14941 atagacccgc ggctggagac tgccgaaccc catggcgccc aaccggcgat gcgccgtcgt
    15001 gagcggcacg acacaaaacg cccgtacccc gttctcgcga aacagcgtca tcaatccggg
    15061 aaagcgatct tcctgcgcaa cgtcatgaac cgtcagcggt tgctgggttt tccacaccaa
    15121 gccgccgggc gattcctcga tgggagtctc gagcccgggg cgaagggtcg agggctccga
    15181 ggtcaccagc agccacaagc gcatcttgtc tgtcgcgggg tcatggagca ccgcgttgat
    15241 gaaatcgaac ggcacgagtt ggggcaaccg tccggccaaa tcctcaagca gtcgggtgag
    15301 gtcacgatgg gaggcgatcg attctgtcac gtccagcagc agttgcagcc gatcgcgctc
    15361 gtcgctcaag gcctggtgat gcagcacatt gtctacggcg actgcgacca gtttggcgac
    15421 ctggttgaga aactccacat cggcgtcacc gaacgcggag gttctcaggc tgccgaatcc
    15481 gatcgcgccg agccgccgca tcgccgtggt gagggggcag aaacagagcg attgcacgcc
    15541 gatgctccgg agcgcgccca gcccgggctt gtaccgcgtt tcatcggcca gcgacgggat
    15601 catcaacggc tgctgatgcg accagaccca gccgctggcg gactcctcca tcgggatttc
    15661 catgccgtcc agccggttcg ttaccccccg cgcctcaacc gtttcaagca catgcacctt
    15721 catgacctgc ttcgccggat cgtgcagcac caggccgatg aaatcgaacg gggcgacagc
    15781 gggcagccgt tgtgcgagat ctcgaaacag ggccgggaga tccctgtgca gagaaatcgc
    15841 ctcggcaacc tgcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacccg
    15961 tttgcccatc tgctgttcga gaacctgccg taggacctcc ggactattga gttccactgc
    16021 cgtctcctca gccaacacgg cagggagtgt gccgaaagac ccgcccagta aacagaggag
    16081 cagcacaatc ctacccggcc tgactcgttc ttcacgacgc agcatagcta cctccttgaa
    16141 tcgcccacat cccgaccaca ggatcgagga ccattgcccc gaagcagttc atgtcgcggg
    16201 gaggatagcc ggggttccgg agaaagtcac gcgcggccga cccggcccac agacctgcgt
    16261 gaagcgcaag aacctcgacg tgcctcggtc tatggcaagg cgaagaaacc gcggcagctt
    16321 tacagatctt gaaggtcagg ctatcatcgt ccaggctcca tacgtacctt cacccaaatc
    16381 cgcaaggaga caccgatggg ctggatcgtc gtcgtcgcgc tggtgctgct cgtgatgatc
    16441 gtcatcggca tgtataactc gctcgtccgt ctccgtgccg cctgcgacag cgcctgggcc
    16501 gatatcgacg tgcagctcaa gcgccggtac gatctgatcc ccaacctcgt ggagaccgtc
    16561 aaggcctatg ccgctcacga aaagggagcg ctggaagctg tgatcaacgc ccgggcgaaa
    16621 gccatgtcgg ctcaggggcc ggaggcgaaa ggcactgcgg aaacgcaact gactcaatcg
    16681 ctgaaatcgc tcttcgcgct tgccgaagcc tacccgcaat tgcgggcggt ggaggccttc
    16741 aatcaactcc aggggagcct caatcagatc gaagacgccg tgcagaacgc caggcgctac
    16801 tacaacgccg tggtccgcga ctacaatgcg aagacccagg agatccccac caacctcatt
    16861 gccggctggt tcaatttcgt cggccgccag ttcttcgaac tcacggacac cacccagcgc
    16921 gagccaccaa aggtgcagtt ttaacgtggg ccgacccgcc ggtccacatg tgagcgccgg
    16981 gcgaagtcca aggcttgcgc cgcgcctgct catcgtcccc ctgctctgct tcctcacctt
    17041 cagccaactc ctccctgcgg cagcccgttc gttcgtgctc agccggttcg acgttgagct
    17101 tcaagtcctg tccggcggcg acctgctggt caccgagacc gtcagccccc gcttcgaagg
    17161 ggcatggaac ggaatcgagc ggctgattcc cgtcgaatat cgaaccccgc aaggcttcaa
    17221 ttacagcctc ctgctggatc gagtcaccgt cacggacgaa cagggcactc ccctcacatt
    17281 cgaaagcagc cgggagcggc actaccggaa tttgaagatc tggattcccg gcgcgaccga
    17341 tgccacccgc acgttcgtct tgaagtatcg cgtgagaaac gggctgaaat ttttcgagga
    17401 tcacgacgaa ctctattgga acgtcaccgg cgacgaatgg gatgtgccca tcga
//
LOCUS       DAOQRM010000044        17306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2394_length_17306_cov_14.067242, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000044 DAOQRM010000000
VERSION     DAOQRM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17306
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2394_length_17306_cov_14.067242"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..576)
                     /locus_tag="PKK81_05510"
     CDS             complement(166..576)
                     /locus_tag="PKK81_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32360.1"
                     /translation="MTNSRSHGAVVLSLSLLLLSACATEETMTGGGSTPSSSAAMKAS
                     AQAYDSLQSCLARIQSGGTAGTRQLAEEGCQRDEALRQSIAGNATAKSGNRAAAGAAG
                     DSLEACMARIPKDGSVGQRMLAEESCQRDQANRR"
     gene            complement(658..2028)
                     /locus_tag="PKK81_05515"
     CDS             complement(658..2028)
                     /locus_tag="PKK81_05515"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNV32361.1"
                     /translation="MARYWSTLILAGVLAGLGLYLYLVELPDQRTEVATATQAKQILP
                     FTEAQITSLTVRSQSGEVVLTHTPGQPWTITAPLQTDADQRQVQALIRALVMGKVSRL
                     VETHPASLAPFGLDHPSTVVTLTAGDKQETLSIGDAGPLSSTLYVLRTSDEAVLLTDL
                     APKDFLNRTLLSFRRKELLQVNQQETERLRLTYPTTEIVLYALDEKPKKKWKIRYPIE
                     AEGDRTEILALLFRLEDLKALSIVDQGPEREKLAPMLTKPKIKVTLSAAGVDHVVRLY
                     QPDPASGEAYAQTTPDGPIYKISPSAIKDLTKDLFTLQDKRLLGVDTGDIAMLSIKTR
                     EEQYVLIHDRNEWILEDQPTEKLRQDVADLLVSRIVNLPAEERVLKQAGPLAPYGLVA
                     PVAEFVATGKDGRVAGRLAMGNQVGGLVYAIGHRIPGIFQVRPDLLKQIPSRLALLGQ
                     GPGSPP"
     gene            complement(2033..3562)
                     /locus_tag="PKK81_05520"
     CDS             complement(2033..3562)
                     /locus_tag="PKK81_05520"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNV32362.1"
                     /translation="MEITRRPLPIGAIGTGLAIAGVITYTLVPDKLWLVTLLEGLALV
                     SLIWAFAIHFERLKTFTSQRSTRMGANSALMVALFLGILAIVNFLAARHSIRWDFSEN
                     QNYTLAPETYRVLRNLTRDISITVFTREKDPGYQAYKERFDSYRQASSKLTVQFIDPE
                     RQPKVAQNYGITRTDIAIFESGPQSVRITSPAEVEITGALVRVSKDTKKRVVFLEGHG
                     ERSLDDTDRGGLTLTKEVLQKQGYDIGTLTLLQESAVPEKTDVLIIAGPRRALTKEEK
                     ERIQAYVVKGGHVLVMVDPDTQSNMDDLLKTWGLELGPGVLVDLQDRLAQGDLTALLV
                     RTFTDHEITHELTAAVLFPLARHLTFQEESGKEWDYVPLARTSPRSWAETDMKGRVVS
                     YTEKEDVQGPLALAAALTPKQAPEEGKPRPAVVVVGNSAFASNGFINFVGNSDFFQRT
                     VGWLSEERDLISLTPKDPAVRPFTPNPLQERILLYVQVILLPAMTILCGLLVWRKRRR
                     L"
     gene            complement(3553..4323)
                     /locus_tag="PKK81_05525"
     CDS             complement(3553..4323)
                     /locus_tag="PKK81_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV32363.1"
                     /translation="MPPVQAIIAKELRSYFVSPIVYVVGAVFLLTFGFLAYLYIVFTG
                     AQAIQLMQMQGGTAQINLNDLVFRNLFASMRFVLLLILPILTMRLLAEERKLRTFEFL
                     MTAPVRVSEIIVGKFISVYLVFLGMLLLTTLVPLTLALFSDFDWYPVLTGYLGMTLLG
                     GFFLAVGLFASSITENQIVAAFTSFGLLLMCWLLAGLGSLLGDTPAGHVVSYLSFMEH
                     FDHLVRGLVDTKDLVYYVSGTVLMLFLAHRVVDSSRWK"
     gene            complement(4328..5296)
                     /locus_tag="PKK81_05530"
     CDS             complement(4328..5296)
                     /locus_tag="PKK81_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV32364.1"
                     /translation="MIEVRNITKRYGNLTAIDRVTFRVDKGEVLAFLGPNGAGKTTTM
                     RILTSFIPATEGTAQVAGFDCAEQPDEVKKRIGYLPETPPVYQELTVSEYLAFVGQLR
                     GLSGAELTAGMDRVMERLSLGSVRQRLIANLSRGYRQRVGLAQALIHDPPVLILDEPT
                     VGLDPKQIIEIRELIKSLAGSHSVILSTHILPEATAVCQRVVIINGGRIVAEDTPDQL
                     SARLRRSEKISVTLKTPPADVLERFREVAGVEHVLTTSEPHTVLIECALGRDIREEVA
                     RFAVGQHWGLLEMKPVSMTLEDVFLKLTQREDGLPKADDASESELH"
     gene            5545..5922
                     /locus_tag="PKK81_05535"
     CDS             5545..5922
                     /locus_tag="PKK81_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32365.1"
                     /translation="MRSDINQPRPSLLGVLVIGAALCVALAGLGSFLTSVYAASNTVH
                     EIKGTVEAVNLADDPPVIVVRGQHGANEATVVGAVLKQGATIVRGKKRIGLDQIHAGD
                     KVTLKYVKTRDGLAVRSIVLHRN"
     gene            5979..6638
                     /locus_tag="PKK81_05540"
     CDS             5979..6638
                     /locus_tag="PKK81_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32366.1"
                     /translation="MIRQHVVRGTSAPVAVGLLLLLGCSGKGDIIPMNLTPKMQTDTT
                     SAVQVVKPMAGPRVMVIPFEDGRPDRTKVGSRSSMWGGDTYFNVSSGSAGEEAAQAFA
                     DYLKRKGWQAQYAKTAPAASEAGPDVVLSGKILELAVDARRGVFLTDIEARTKLVIQA
                     SNREDNSSLVRTDAHSGSHDNVLWFEPQDGESILSEVLEKNFERFVVNTKFEDRGIRF
                     R"
     gene            7066..7650
                     /locus_tag="PKK81_05545"
     CDS             7066..7650
                     /locus_tag="PKK81_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNV32367.1"
                     /translation="MSACSALPKGELTLDLGIKDRGVASWYGKEFHGKLAANGEAFDM
                     TAYTAAHRKLPLGSVVRVVNLTNGKTVQVRINDRGPYVAGRMLDLSHAAARELGMVEA
                     GTTAVQIEVIGDHRPVAQIPSTKIPAVSGMVLNTDMRAVRQHRRPEEGVERPAVPVRM
                     MPQEALYVRRERRIGSMLAADHTAHNTVPVLILS"
     gene            7778..7981
                     /locus_tag="PKK81_05550"
     CDS             7778..7981
                     /locus_tag="PKK81_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32368.1"
                     /translation="MAKKPVGESDEVRLKKKIAVRVTKEAKLSGDPAARSLHKRLKRV
                     QRKRRALALRKKHAAGNQTEAKS"
     gene            8046..8876
                     /locus_tag="PKK81_05555"
     CDS             8046..8876
                     /locus_tag="PKK81_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV32369.1"
                     /translation="MEHTGEKEEQAQAVSPISQVEDETLTDQVSDQLAASLDSSAGAQ
                     DLTDPLQEVVLEEEQVKDEIDIQIDLLKDPDWVVRREAAITLGEMGDERCVEPLAAAL
                     RDGDWQVREVAIEGIGQIGSPAVEVLLKLLRDWDVRKSAIMALGKIRDERVLEPLMQQ
                     LRNDEFMEDATDALVNLGEPALPGLIKALKDKEELVRKQAVIALGRIKSPEAIDPLIE
                     MLQNKDWFTRLTAAAALEAIGDERGREAIKPLLKDSDMVVKMRVERILAKWKKQPAAV
                     "
     gene            complement(8885..9319)
                     /locus_tag="PKK81_05560"
     CDS             complement(8885..9319)
                     /locus_tag="PKK81_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32370.1"
                     /translation="MLRFIGLLMLLALSFGFGYLWGKQPANNLEQTVRDFSRNVVDTT
                     LGVERDLHRRQSLVDAKSKVVQAKAELYNKNTTNAAKELAAAIDSLELATRGGKQVDP
                     NAQVKELAGKIRELRLELSLGKKVSATKLDDVQKELDLLLRK"
     gene            complement(9335..10585)
                     /locus_tag="PKK81_05565"
     CDS             complement(9335..10585)
                     /locus_tag="PKK81_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV32371.1"
                     /translation="MTLYQAAEYSRVLELLQQIPAGQQPALELLRYGILSHLKLGKPE
                     EAWKLYTKLVADNRPDEPAVLRDIARSFIVTRVRDTQEHIRIAAYTALAEMGESDTLP
                     ILEDGLLDSSALVRARAAEAIGRAGLAGRSSALKRALRDEAPGVRIAAINALSDAKVA
                     GITDQLTEIARVDEGPESIFAFAGLYKLGRTDVLTDISNAVTLPDPEVRMAALGVLGR
                     LRRPASLSILSQAVYDPHPAVRAFAAGALGEFGSTDGIAPLTHALSDENPRVRSVAAS
                     SIGRLGATETRSVVQPLLRDPDEHVRISAVEALLRLGDASAVLNAADLARHPDPSVRG
                     GAAHALAAATTPNALTVLESLLRDQQPLPRLMAARALGKLQQKSLISPLKTGLQDSDP
                     TVRIASAGSLIHILSRKDKQGSAH"
     gene            complement(10743..12902)
                     /gene="glgP"
                     /locus_tag="PKK81_05570"
     CDS             complement(10743..12902)
                     /gene="glgP"
                     /locus_tag="PKK81_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNV32372.1"
                     /translation="MQAPDHIQPPQDTVPQSLHRLGELARNLWWSWNQPARQLFESID
                     PTLWFLTHHNPVQLLSGVKPERFVTLAGDPNFVRQYSAVLRAFDEYLSNKNTWAATEF
                     SALQNSPIAYFSAEFGLHISIPIYSGGLGILAGDHCKEASDLGIPLVGIGFMYPQGYF
                     KQRINPEGWQEATYAPFNRYDSPIHQAMTPTGIPCSIKVEIGHRQVTVLVWQVRNGRM
                     SLYLIDTDVPENTPEDRALSARLYGGDQEIRLCQEFLLGIGGVRILRALGINPAVWHC
                     NEGHSAFLTVERIREFVTTGHSHAEASDLVRQSTVFTTHTPVPAGHDIFPFHLMDRYF
                     SNYWGQLGLSREEFLRLGETPENAGHGFNMTALAMRLSAHVNGVSREHGRVSRQMWQH
                     LWPGLAQDLVPIRSLTNGIHAPTWISPESNSLYAKYLSPDWAERVDDPTIWQRVTDLP
                     DDALWAVRQTTRRKLMRFIRERARDGWIRGHLQPMQAIARGTLLDPEALTIGFARRFA
                     TYKRATLLFRDLDRLKQLLHNRWRPVQIVFAGKAHPADEPGRYFIHEVLSFCNDHKLG
                     GHVAFLEDYDMHMAKYLVQGVDIWLNTPRAPLEASGTSGQKAALNGVINLSVLDGWWQ
                     EGYNGANGWGIQPLPEGSDTQAQDAHDAEQLFRLLEQEVVPLFYQRDLDGIPRGWLQI
                     VKESIKTVAPRFCTKRMVKEYMRQLYAPATARTPNKW"
     gene            complement(12972..13808)
                     /locus_tag="PKK81_05575"
     CDS             complement(12972..13808)
                     /locus_tag="PKK81_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32373.1"
                     /translation="MTTRPHSFAQQIQTVAELMLAVAGESVSVIKRAAPEQALKLKHQ
                     DEWTIYLEFLRVMFNLTDRVSALHIPLKEQPQFMDELTDTVIDQLKKALEPAFGAGSD
                     QTEIVLTIGTAVAESRQTYERYRFLVTEDSPAKNDMYRDFSDRVARAVGVPRSPGVTA
                     AATLCVSAVIPALIGIFEGQAPPVAADAAANPATAGGSSAPRGATGADIKLVSVMSSI
                     KGEEVETRWGLHPRFRQDLTQEEAKQLTTTMNRVAKILGERYATVAFSDQWASWHKAG
                     HA"
     gene            13930..16014
                     /locus_tag="PKK81_05580"
     CDS             13930..16014
                     /locus_tag="PKK81_05580"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNV32374.1"
                     /translation="MLYSATAPVMSLTAQSDANMDRESKPYILKRTPDQDHVRKFSLD
                     YAKELNAQQYAAVTAADGPALVIAGAGSGKTRTLVHRVAYLIDSGVDPSHILLLTFTR
                     KSSEEMLERVGALIGSRSQRVCGGTFHSVANMLLRRHGRVLGIEPGFTIMDRGDAEDL
                     IALLRAQLGLNEKDKRFPRKGTIAEIYSKCENTLRGLEEIVLDEFSHFADHLEALGKL
                     QRAYQAAKRQRQLLDYDDLLVLLRDLLTKDEPIRRTISQQFRYILVDEYQDTNRLQAE
                     LIRKLAATHDNVMVVGDDSQSIYAFRGATFRNIMEFPSWFPGAKIYKLEENYRSTQPI
                     LSLANEIIQEAPEKYTKHLFTRKLDGPLPALVEAAGENAQSRFVAQKILELREEGVPL
                     SEIAVLFRSSFHSFDLEIELSRCGLPFVKRGGIKFIEAAHVKDLLAHLRVVVNPQDAV
                     SWHRVLMLVEGVGPKKAQDLVAAMVRVNDPYQVLWDSSGRSGKGLKELALVLDNLSKS
                     DDLSPTEQVNRVYEYYLPILKDHHDDYPKRIRDLDHLHTIAESYPSLAEFLADLALAP
                     PDGSAVGVEPAGRDDEQVVLSTIHSAKGLEWQCVFLLWVVDGKFPSVFSFNTDEELEE
                     ERRLLYVAVTRAKRYLFLTYPINVYDRSSGMLLSKPSRFLDHVSPRLFETLALVEEGH
                     GHEWGHEHDRYV"
     gene            16081..17016
                     /locus_tag="PKK81_05585"
     CDS             16081..17016
                     /locus_tag="PKK81_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32375.1"
                     /translation="MRPVLVYNGWFRAVLPTMAAGLVLGLLIFGQASAETAAPSSDSS
                     RLWGALMAEAKALHLPTRFLEQIPAQFVAFEFEDLHAFAAEYHPVEHRMVLDRSLSLN
                     AAGRTLRPLARLTHKELETLYHELFHAYMDFLEQERAPSAAHASFMAFAREQQRCRYQ
                     QVLITPVLQKKDLKEERFLSEAESWEALNETWAVFVGWAIWTQLEVERKGARELRSSG
                     RSLNVPAWIARLSQAEQDASLRGYYEPEDPSEKAMARKRFLAPDFRLSAPELLLLMKE
                     VLGSPADMTRQADQVLTRGRVVPASRGACEAPAAS"
BASE COUNT         3505 a         4518 c         5407 g         3876 t
ORIGIN      
        1 acgtgcgacc cgcggtttag gaaaccgctg ctctatccaa ctgagctacg ggggcgtgag
       61 gatacgatac tgaaagcgaa gaggattgtc taccttaagt gacgcgcctc cgaaggggcg
      121 gctgccacct tcggaggtga gtcggtctcg agaagcgaga gggtgctagc gtcgattggc
      181 ttggtcgcgc tggcagctct cttcggccaa catccgttgc ccgactgagc cgtccttggg
      241 aatgcgtgcc atgcaggctt caaggctatc acctgcagca ccggccgccg ccctgtttcc
      301 actctttgca gtggcattcc cggcgatcga ctgacgaagc gcctcatccc tctgacagcc
      361 ttcttcggcg agctgacgcg tgcccgccgt gccgccggat tggatgcgtg ccaggcagga
      421 ttgcagtgag tcataggcct gcgcggaggc tttcatggca gcgctcgaag atggtgtcga
      481 tccgccgccg gtcatggttt cttccgtagc acaggcactg agtaacaaca gagataagga
      541 cagaactaca gcaccgtgag aacgcgagtt cgtcattgaa ggatcctcct tgatcaagag
      601 cgatgggcgc acagtagcat aaaccttctc tatctggaaa cacctccgga aggtcgttta
      661 ggggggtgac cccggaccct ggcccaggag tgcgagtcgg gagggaattt gtttcagcag
      721 gtcggggcgt acctggaata tccctggaat tcgatggccg atggcataca cgagtccgcc
      781 gacttgattg cccattgcga ggcgtccggc gacgcgcccg tccttcccgg tagcgacgaa
      841 ttcagccacg ggagccacga ggccataggg cgccagcggg ccggcttgtt tcaggacacg
      901 ttcttccgcc ggcagattga cgatacggct gacgagcagg tccgcgacgt cctgacgaag
      961 cttctctgtc ggctgatcct cgaggatcca ttcgttccgg tcatgaatga gcacgtactg
     1021 ctcttcgcgt gtcttgatcg agagcatggc gatgtcgccg gtatccactc cgagcagtcg
     1081 cttatcctgg agggtaaaga ggtctttggt caggtccttg atcgccgagg ggctgatttt
     1141 gtatatcggg ccgtccgggg tggtttgggc ataggcttct ccacttgccg ggtcgggctg
     1201 atagaggcgg accacgtggt ccaccccggc cgcactcaag gtgactttta ttttgggttt
     1261 ggtcagcatg ggtgcgagct tttcccgctc aggcccctga tcgacgatcg agagcgcttt
     1321 aagatcttcc aatcggaaga ggagggctag gatctcggtg cggtccccct cagcttcaat
     1381 gggatagcgg attttccatt tctttttcgg cttttcgtcc aacgcgtaga gcacgatttc
     1441 tgttgtggga taggtgaggc gcagtcgctc cgtctcctgc tgattgacct gcaacaattc
     1501 cttgcggcgg aatgagagca gggttcggtt caggaagtct ttaggggcca gatccgtcaa
     1561 gagcacggcc tcgtcggacg ttctcaggac atagagggtg gaggacaggg ggccggcatc
     1621 gccgatcgag agcgtttcct gcttgtctcc tgcggtcagg gtgacgactg ttgaagggtg
     1681 gtcgaggccg aacggagcca atgaggctgg atgtgtttcg acaaggcgcg agaccttgcc
     1741 catgaccaac gcgcggatca aggcctgcac ctgccgctgg tcggcgtcgg tttgaagggg
     1801 tgcggtaatg gtccagggtt ggccaggtgt gtgagtcaat accacctcgc cggattggct
     1861 gcgaaccgtc agcgaggtga tctgtgcctc ggtgaacggc agaatttgtt tggcttgggt
     1921 ggcggttgcc acttcggtac gttggtcagg gagttcgacg aggtagaggt acagtccaag
     1981 ccctgccaac acgcctgcca gtatgagcgt cgaccagtag cgcgccatgg tcttacaaac
     2041 gacgccgctt ccgccacact aggaggccgc acaggatagt catcgccggg agcagaataa
     2101 cctgaacgta gagcagaatg cgttcctgga gcgggttggg ggtaaaggga cgcacagcag
     2161 ggtctttggg cgtgagggag atgagatccc gttcttccga gagccatccc accgtgcgtt
     2221 gaaagaaatc gctgttgccg acgaagttga taaacccgtt gctggcaaat gcggagttgc
     2281 cgacgacgac tacggcgggg cgcggtttgc cttcttcggg cgcttgcttg ggggtcaagg
     2341 ctgcagccag cgcgaggggg ccttggacgt cttccttttc ggtgtagctg acgacgcgtc
     2401 ctttcatatc ggtttcggcc cagctgcgcg gcgaggtgcg cgcgagagga acatagtccc
     2461 actctttgcc tgattcctcc tggaacgtga gatggcgagc caggggaaac agcacggccg
     2521 ccgtcaattc atgggtgatt tcgtggtcgg taaacgtgcg gaccaagagg gcggtcaggt
     2581 ctccctgcgc cagccggtcc tgtagatcga ccaggacccc cggccctagt tcgagtcccc
     2641 aggttttgag caggtcgtcc atattcgact gggtatcggg atcgaccatc accaggacgt
     2701 gtcccccctt caccacgtag gcctggatgc gttccttctc ttctttggtc agcgctcgcc
     2761 gtgggccggc aatgatcagg acatcggttt tctcaggcac tgcggattcc tgcagcaagg
     2821 tgagggtgcc gatgtcatat ccctgctttt gtagcacttc tttggtcagc gtcagcccgc
     2881 cgcgatcggt atcgtccagg ctccgctcgc catgcccttc cagaaacaca acccgcttct
     2941 tggtatcttt ggacacccga acgagcgcgc cggtaatttc tacctcggcc ggggacgtaa
     3001 tgcgaaccga ttgcgggccg ctttcaaaga tcgcgatatc ggttctggtg atgccatagt
     3061 tttgtgcgac tttcggctga cgctcggggt cgatgaattg gacggtcagc ttggaactgg
     3121 cctgccgata actgtcgaag cgttccttgt acgcctggta ccctgggtcc ttctcccgcg
     3181 tgaacactgt gatgctgata tcccgcgtga ggttgcgaag gacgcgatag gtttccggcg
     3241 cgagcgtata gttctgattc tcggagaagt cccagcggat cgaatgtcgc gccgcgagga
     3301 agttgacgat ggcgaggatg ccgagaaaca gcgccaccat gagcgcactg ttggcgccca
     3361 tgcgggtcga tcgttgggag gtaaaggtct tgagccgttc gaagtggatg gcaaaggccc
     3421 aaatgagcga gaccagggca agcccttcca gtagcgtcac gagccacagt ttgtccggta
     3481 ccagggtgta ggtgatcact cccgcaatgg ccagcccggt tccgatggcg ccgataggaa
     3541 gggggcgacg agttatttcc atcgtgacga atccaccact cggtgcgcga ggaatagcat
     3601 taaaacggta ccactgacgt aataaaccag atcttttgtg tcgaccaagc cacgaaccaa
     3661 atgatcgaag tgttccatga acgagagata ggagaccacg tggccggccg gcgtgtcacc
     3721 taacagcgag ccgaggccgg ccaacagcca acacatcaag agcaggccga agctggtaaa
     3781 ggcggccacg atttgattct ctgtgatgga tgaggcaaag aggccgacgg ccagaaagaa
     3841 ccctccgagg agtgtcatgc ccaggtagcc ggtcaggacg ggataccaat cgaaatcgct
     3901 gaagagggcc agtgtgagcg gaaccagggt ggtcaggagc agcattccga ggaacacaag
     3961 gtacacgctg atgaatttcc cgacaataat ctcactgacc cgcacggggg cggtcatcaa
     4021 aaattcgaag gtccgcagtt ttcgttcctc ggccaggagt cgcatggtga gaatagggag
     4081 gatcaacagc aacacgaagc gcatgctggc aaagagattg cggaagacca gatcgttcag
     4141 attgatctgc gcggtgccgc cctgcatctg catcaactga atggcttgag ctccggtaaa
     4201 cacaatatag aggtaagcga ggaagccaaa ggtgagcagg aagacggcgc cgacgacata
     4261 gacgatcggg gagacgaaat aggatcgcag ttctttcgcg ataatggctt gaactggtgg
     4321 catcgggcta gtgtagttca ctttcgcttg catcgtccgc tttcggcaga ccgtcttccc
     4381 gctgagtcag tttcaggaag acgtcttcca aggtcataga gaccggtttc atctcaagca
     4441 atccccaatg ctgtcccact gcaaaccgtg cgacttcttc tcgtatatct ctgccgaggg
     4501 cgcactcgat gagaacggtg tggggctcag acgtcgtcag cacatgctcg accccggcga
     4561 cctcgcgaaa tcgctcgagg acatctgccg gtggagtttt gagggtcacg cttatttttt
     4621 ccgatcgtcg gagcctggcg gacagttggt caggcgtgtc ttcggcgacg atccggccgc
     4681 cgttgatgat cacgacacgc tgacagacgg ccgtggcttc agggagaatg tgggtgctca
     4741 aaatcactga gtgagagccg gccaggctct tgatcaattc tctaatttcg atgatttgtt
     4801 ttgggtcgag acctaccgtg ggttcgtcca ggatcagaac cgggggatcg tgaatcagcg
     4861 cttgggccag gccgactcgc tggcggtacc cgcgtgacag attggcgatg agtcgctggc
     4921 ggacagatcc taaggaaagc cgttccatga cacggtccat gccggccgtc agctctgcgc
     4981 cactgagtcc gcggagttgt ccaacgaagg cgaggtattc ggagacagtg agttcttgat
     5041 acaccggcgg cgtctcgggt aaatagccga ttcgcttttt cacctcatcc ggttgctctg
     5101 cacaatcgaa ccctgcgacc tgcgctgtcc cttccgttgc cggaatgaaa gaggttagga
     5161 ttcgcatggt cgtggttttt ccagcgccat tcgggccgag gaatgccagc acctcacctt
     5221 tgtccacacg aaaggtgacg cggtcgatcg ccgtcaggtt gccataccgt tttgtgatgt
     5281 tgcgaacttc gatcatgcag tcgagagaga cggctggatg ggtgtgattc ctgctgttcg
     5341 ttgatacggc acgtggtgtg gcaaatcgat ctggatatta ccgaccgcct tcggtgcagt
     5401 caagagacgg atgtggctcc tggctttccg ggacggcagg aaaagacctc gtccgtcttt
     5461 acagcgtgat gtgtaactgc taaagttccg cgactttatc catcaagttg ctccgcacaa
     5521 tgaatgaaaa gaggttcgtc tgggatgagg agtgatatca atcagccgag accgagcttg
     5581 ctcggtgtgt tagtgatcgg cgccgcactc tgtgtcgccc ttgccggcct cggaagtttt
     5641 ctgacctcgg tctatgctgc cagtaacacg gtgcacgaga tcaaaggaac ggttgaagcc
     5701 gtcaacctag cagatgaccc tccggtcatc gtggtcaggg ggcagcacgg agcaaatgaa
     5761 gccacggtgg tcggtgcggt gctcaagcag ggtgccacca tcgtgcgcgg gaaaaagcgg
     5821 atcggactcg atcaaattca tgcgggagac aaagtgacgc tgaagtatgt caaaactcgt
     5881 gacggcctcg ccgttcgatc aattgtgctc caccgcaatt aggcaatgat tagtcaccag
     5941 ttatggattt gttactcaca aggaggtaga gagattttat gattcgacag catgtggtgc
     6001 gggggacatc tgcgccggtg gccgtcgggt tgctgctcct gttgggctgc tccggaaaag
     6061 gcgacattat tccgatgaac ctgaccccca aaatgcaaac ggataccact agtgcggttc
     6121 aggtggtgaa gcctatggcg gggcctcgag tgatggtcat tccgtttgaa gatgggcggc
     6181 ccgatcgaac caaggttggg agtcggagtt cgatgtgggg aggcgacaca tactttaacg
     6241 tgtcgagcgg tagtgctggc gaggaagccg cccaggcttt tgccgattat ctcaagcgca
     6301 aaggatggca agcgcagtac gccaagactg ccccggcagc atcagaagcc ggtcccgatg
     6361 ttgtgctttc cgggaagatt ctcgaactgg cggtcgatgc ccggcgcggg gtatttctca
     6421 ccgatattga agcccgaacc aaactggtca ttcaggcgag caaccgagaa gacaacagct
     6481 cactcgtacg aaccgacgct cattcaggta gccatgacaa tgtgttgtgg ttcgagccgc
     6541 aggatgggga aagtatcctg tcagaagtgt tggagaagaa tttcgagcgg ttcgtagtaa
     6601 acaccaaatt tgaagaccgg ggtattcgat ttcgttagat cgtgttgcca gtgggaaatc
     6661 gaactgtcgg gctggcctga ttttatacgg ttgatccgag agcccgacaa tttgggcttg
     6721 tgttcactct ggaactatca gcagttccaa gcgttgaggc atcctgcgta tcagtgagcg
     6781 gaagaaattc atttgtgtcg ttgttcggct ctgttcagca aaatcataga cttcttcttc
     6841 gttgagcgtt gaattgtctg cccatttgta gggggcgtct gctacccaga cggtattgtc
     6901 gccaagggca atcttgcgta gcctgacctc actgagtagg agagttgtct gacaggcggt
     6961 gaggtgtagg ctttcacacg aggtgcttct atggacatga actcccggaa ccctttccgt
     7021 cgtaacgccg gttacgctgt gattcctctg gctggctggc ttctcttgag tgcttgttcc
     7081 gcgttgccga aaggcgagct tacattggac ctcggcatta aagatcgggg agtggcctca
     7141 tggtacggca aggagtttca tggaaaactc gcagcgaatg gggaggcctt cgatatgacg
     7201 gcctacactg ctgctcatcg caaactgccg ttaggtagtg tggttcgtgt ggtcaatttg
     7261 accaatggga aaaccgttca agttcgaatc aacgatcggg ggccttacgt tgcagggcgt
     7321 atgctggatc tttcgcatgc ggcggctcga gagttaggca tggttgaagc cggtaccacg
     7381 gcggtgcaga ttgaggtgat tggagatcat cgtccggtcg cgcagattcc ctcgacgaag
     7441 attcctgctg tgtccggtat ggtattgaat acggatatgc gagcagtgag gcaacatcgt
     7501 cgcccggagg agggagttga aagacctgcc gttcctgtaa gaatgatgcc acaagaagcg
     7561 ctctatgtga ggcgtgagcg ccgtatcggc agtatgttgg cagccgacca cacagctcac
     7621 aataccgtcc ccgtcctcat cctatcctaa gtgggcggta ctccgttagt tgacacttcc
     7681 gcccgtactt gattagactg cctcctctgg agtgatgcct gattgcgccg tcatttctta
     7741 tgtttgttga tcttaattgt tggaggttga aagctgaatg gcaaagaaac cagttggtga
     7801 atcagacgag gtccgactca agaaaaaaat tgcggtacga gtaacaaaag aagcaaaact
     7861 ttcaggtgat ccggctgccc gctctttaca caaacggctc aagcgagtgc agcgcaaacg
     7921 gcgtgcgctt gcgttgagaa aaaagcatgc tgctggaaat cagactgaag cgaagtccta
     7981 agcggttgcg ggattgacgg atttgatcgc cacgcccacc cctctgcgga acaaggaaca
     8041 ttgatatgga acacactggt gaaaaagaag aacaggccca ggctgtttcc cccattagtc
     8101 aggttgagga tgagactttg acagatcagg tctcagatca actggctgct tccttggatt
     8161 cttcggcagg cgcccaggac ctcactgatc cattgcagga agtggttctt gaggaggagc
     8221 aggtcaagga cgagatcgat attcaaatcg atcttctcaa ggatcctgat tgggtggtca
     8281 ggcgagaagc tgcaatcacg ctgggagaaa tgggcgatga gcgttgcgtc gaaccgcttg
     8341 ctgcggctct tcgcgatggt gactggcagg tgcgggaagt tgcgattgag gggatcgggc
     8401 agatcggttc tcctgccgtc gaggttttgc ttaagttgct cagagattgg gatgtgcgga
     8461 agtccgcaat catggctctg gggaaaattc gtgatgaacg ggtgcttgag cctctgatgc
     8521 aacagctccg aaatgatgag ttcatggagg atgccaccga tgccttggtt aatcttggtg
     8581 agccggctct gcctggtttg atcaaggcgt tgaaagacaa agaagagtta gtgcggaagc
     8641 aagcggtgat tgcgttgggg cggattaagt ctcccgaagc catcgatccg ctgattgaaa
     8701 tgttgcagaa taaggactgg ttcactcggc tgacagctgc cgcggcactt gaagctatcg
     8761 gcgatgagcg tggtcgcgaa gcgatcaagc cattgctgaa agacagtgat atggtggtta
     8821 agatgcgagt tgagcgtatt cttgcaaagt ggaagaagca accggccgct gtttaacgac
     8881 cgcttcactt acggagcagg aggtctagtt ctttttggac gtcgtcgagc ttcgtagcag
     8941 aaaccttctt tccgagggat agttccaagc gaagttctct gatttttcct gccagctctt
     9001 tgacctgagc attaggatct acttgtttcc ccccacgggt ggctagctcg agtgagtcga
     9061 tagccgctgc cagttccttt gcagcattgg ttgtattttt gttgtaaagt tcagccttcg
     9121 cttgcaccac tttggatttt gcgtcgacga ggctttggcg gcgatgcaga tcccgttcta
     9181 ctcccagggt ggtatccacg acattccgcg agaaatcccg caccgtttgc tcaagattgt
     9241 tcgccggctg tttgccccag aggtatccga atccgaacga gagggctagt agcatcaata
     9301 gccctataaa tctgagcata ctgattctca gcccctagtg ggcgctccct tgtttgtctt
     9361 ttcgagacaa tatgtggata aggcttcctg ctgaagctat ccgaaccgtg ggatccgaat
     9421 cttggaggcc ggttttaagt ggagagatga gggatttctg ttgcagtttg cccagcgccc
     9481 gtgcggccat gagtcgtggg agcggctgct ggtctcgaag taagctttcg agcacggtca
     9541 gggcattggg ggtggtggcg gccgccaggg catgcgcagc acccccacgt actgagggat
     9601 cgggatgtcg ggcgaggtct gcggcattca gaactgcgct ggcatcaccg agtcggagga
     9661 gagcttcgac ggcgctgata cgaacatgct catcggggtc tcgcaatagc ggttggacaa
     9721 cgctacgagt ttcagtggcg ccaagtcgac cgatactgga agcggcgaca ctgcggacgc
     9781 gcggattctc atcactcaga gcgtgtgtca gtggagcgat cccgtcggtg ctaccgaatt
     9841 ctcctagagc ccccgctgcg aaggcacgga cggcaggatg gggatcgtag acggcttggc
     9901 tcaagatcga caggctggca ggtcgacgca atcgtccgag cacacccagt gctgccatgc
     9961 gcacctcagg gtcggggagc gtgacggcat tggagatgtc ggtcaggacg tccgttcgtc
    10021 caagcttgta caatcctgcg aaggcaaaga tggattccgg accctcatcg acgcgggcga
    10081 tttcagtcag ttgatccgtt atgccggcga ctttggcatc actgagggca ttgattgccg
    10141 caatgcgaac tccgggcgcc tcgtcgcgca gcgctcgttt gagcgcagag gagcgtccgg
    10201 ccaggccggc tcgtccaatg gcttcggctg cgcgagcgcg gacgagcgcc gatgaatcca
    10261 acaggccgtc ttcgagtatc ggtaacgtgt cgctttcgcc catttccgcc agcgccgtat
    10321 aggcagcaat gcggatatgt tcctgagtat cacggacacg agtcacaata aagcttcgtg
    10381 caatgtcccg caatacggct ggttcgtctg gacgattgtc cgcaacgagt ttggtgtaga
    10441 gcttccacgc ttcctcaggc ttaccaagtt tgagatgact cagaatgcca taacgcagga
    10501 gttccaacgc tggttgctgg ccagcgggta tctgctggag tagctccagc acgcggctgt
    10561 attcagccgc ctggtagagc gtcatggctt gtcgttcgac gggggtgacg gcgggcttgg
    10621 tcgctgatgg gactgccgtt gcggaatgcg ggatggccca ggcaagcagc gcaagcccaa
    10681 ctggcagtac ccaggtcgtg tgtcggcgca ctcgagcgag ctccggccca ggctcggtca
    10741 tgctaccact tgttcggggt gcgcgcggtg gccggtgcat acagttgcct catgtattcc
    10801 ttgaccatcc gtttggtgca gaagcggggg gcgacggttt tgatgctctc cttaacaatc
    10861 tggagccatc cgcgtggaat gccatccaag tcgcgttggt agaacagcgg tacgacctcc
    10921 tgttccaaca accggaacaa ttgctccgca tcgtgagcgt cctgcgcctg ggtgtccgat
    10981 ccctcaggca ggggttgaat gccccatccg ttggcgccgt tgtacccctc ctgccaccaa
    11041 ccatcgagga cactgaggtt aatcacgccg ttgagcgcgg ccttttgacc gctggtcccg
    11101 ctcgcctcga gtggggctct cggagtgttc agccagatat cgacgccttg caccaggtac
    11161 tttgccatat gcatgtcata gtcttcgagg aaagcgacgt gaccaccgag tttgtgatcg
    11221 ttgcagaaac tcagcacctc gtgaatgaag tagcgcccag gttcgtcggc cgggtgcgct
    11281 ttgcctgcaa acacgatctg gacgggccgc caccgattgt ggagcaactg tttgagtcgg
    11341 tcgagatcgc ggaataggag tgtcgcacgt ttgtaggtcg cgaaccgtct ggcaaatccg
    11401 atcgtgaggg cttccgggtc cagcagcgtt cccctggcga tcgcttgcat gggctggaga
    11461 tggccgcgaa tccagccgtc ccgcgcgcgc tcgcgaataa accgcatcag tttgcgtcgc
    11521 gttgtttgtc gaacggccca aagagcgtca tccggcaggt ccgtgacccg ttgccaaatg
    11581 gtggggtcat cgacccgttc cgcccagtca gggctgagat atttggcata gagggagttc
    11641 gactccgggg agatccaggt gggcgcgtgg atgccgttgg tgaggctgcg gatagggacg
    11701 aggtcctgcg ccaggccagg ccacaagtgt tgccacattt gtcgagagac tcgcccgtgc
    11761 tcgcggctca cgccgttgac gtgggccgac agccgcatcg caagggcggt catgttgaag
    11821 ccgtgtcctg cgttttccgg cgtttcgccc aggcgtagga actcttctct ggagagtccg
    11881 agttggcccc aataattgct gaagtagcga tccatcaggt gaaacggaaa aatatcgtgc
    11941 ccggccggaa cgggggtgtg ggtggtgaag acggtgctct gtcgaaccag gtcactcgct
    12001 tcagcatgac tgtgtccggt ggtgacgaat tcccggatgc gttctacggt caggaaggcc
    12061 gaatggcctt cattgcaatg ccagaccgcg ggattgatgc ccagggcgcg caagatccgc
    12121 acgccgccga tcccgagcag aaactcctga cagagcctga tttcctggtc tccaccgtag
    12181 agcctcgcgg agagggcgcg atcttcgggc gtgttttccg gcacatcggt atcgatgaga
    12241 tagagggaca ttcgtccgtt acgtacttgc cacaccagca ccgtaacctg gcggtggccg
    12301 atctcgacct tgatggagca ggggatcccc gtcggcgtca tcgcctggtg aatcggcgaa
    12361 tcgtatcgat tgaagggggc gtaggtggct tcctgccatc cttccgggtt gatacgctgc
    12421 ttgaaatatc cttgcgggta catgaagccg atgccgacga gaggaatgcc caagtcgctg
    12481 gcttctttgc aatggtctcc ggcaagaatg ccgagtccgc cgctatagat tggaatagaa
    12541 atgtggagtc cgaactcggc cgaaaagtag gcgatgggag aattctggag tgcggagaac
    12601 tccgtcgccg cccaggtgtt cttattactc agatattcgt cgaaggcgcg taacaccgcg
    12661 gagtattgcc ggacgaaatt cggatcgccg gccaaggtta caaagcgttc aggtttcacg
    12721 ccggagagca gctggaccgg gttgtgatgg gtgaggaacc agagtgtagg gtcgatggac
    12781 tcaaacagct gacgtgccgg ttggttccaa ctccaccaga gattgcgggc cagttcaccg
    12841 agtcgatgca ggctttgggg aaccgtatct tgagggggtt gtatgtggtc gggtgcttgc
    12901 acgacgcctc cgtggaatgt gatcgatggg tgaggaaggg gactgtgttg tcccgtttcc
    12961 tgaatgctgc gtcaggcgtg tccggctttg tgccaggatg cccattggtc agaaaaggcg
    13021 acggtggcgt agcgctctcc gagaattttg gcgactcgat tcatggtcgt cgtcagttgt
    13081 ttcgcctcct cttgcgtcag gtcctggcgg aaacgcggat ggagccccca tcgcgtctcg
    13141 acctcctcgc ctttgatgct cgacatgaca ctcaccagct taatatcggc gccggtggct
    13201 ccccgaggcg cgctgcttcc cccagccgtg gccgggttgg ctgccgcatc ggcggcgacg
    13261 ggcggcgctt gcccttcgaa aatcccgatg agggccggaa tgacggcgct gacacagagc
    13321 gtggccgcgg cggtcacacc cggactgcgt ggaactccga ccgcacgggc gacgcgatca
    13381 ctgaaatccc ggtacatgtc gttcttcgcc gggctgtctt ccgtcacgag aaaccggtag
    13441 cgctcatagg tctgtcggct ttcggccacg gccgttccga tggtcagtac gatttccgtc
    13501 tggtcgctgc cggcaccgaa ggccggttcc agggctttct tgagttggtc gatgaccgtg
    13561 tcggtcaact cgtccatgaa ttgcggttgc tccttgagcg ggatgtggag cgctgacacg
    13621 cggtcggtca ggttgaacat gacgcgcagg aattcaaggt aaatggtcca ctcatcctgg
    13681 tgtttgagct ttaaggcctg ttccggagcc gcccgtttga tcacggagac ggactctccc
    13741 gcgaccgcga gcatgagctc cgccacggtt tggatctgtt gagcgaacga atgcggtctg
    13801 gtcgtcataa aagtcccctg ctggtccggc attatataca actcaatttg aaggtccaag
    13861 tgttgatctc gatggacgcg atcggagcaa gcgactcacg aactgtgtcg ttgcaaggtc
    13921 tccggtggta tgttatacag cgcgaccgct cccgtcatga gcctcactgc ccagtcggat
    13981 gccaacatgg accgagagag taagccctac attctgaaac ggactccgga tcaagaccac
    14041 gtccggaagt tctcgttgga ttatgccaag gagttgaacg cgcagcagta tgcggccgtg
    14101 acggcggccg atggccctgc gctggtcatt gccggtgccg gaagcggcaa aacgcggacg
    14161 ctcgtgcatc gcgtcgccta tctgatcgat tccggggtgg atccctcgca cattttgctg
    14221 ctgaccttca cgaggaagtc ttcggaggag atgctggagc gtgtcggcgc tttgatcgga
    14281 tcgcgtagcc agcgagtctg tggcggcacc ttccattcgg tcgccaacat gctgctgcgg
    14341 cgccatggac gagtcctcgg gatcgaaccg ggctttacga ttatggatcg cggcgatgcc
    14401 gaagatctga tcgccctctt gcgtgctcaa ctgggcctga acgaaaaaga taagcgattc
    14461 cctcgcaagg gaaccattgc ggagatttac agcaagtgtg agaacacgtt gcgaggattg
    14521 gaagagattg tcctcgacga gttttcccac tttgcggacc atctggaggc gctggggaag
    14581 ctccagcgcg cctatcaggc tgcgaagcgc cagcgccagt tgcttgatta tgacgatctc
    14641 ctggtgctgt tgcgggactt gttgaccaag gacgaaccca tccggcggac catctcgcaa
    14701 caattccgct acatcctagt ggatgagtat caggacacga atcggctgca ggcggaactc
    14761 attcgaaagc tggcggctac tcacgacaac gtcatggtcg tgggtgacga ttcacagtcc
    14821 atctatgcgt tccgcggtgc gacgttccgc aacatcatgg agtttccctc ctggttcccc
    14881 ggcgccaaga tctataaact ggaggaaaat taccgcagca cgcagccgat tctcagcctg
    14941 gccaacgaga tcattcagga agcccctgag aaatatacga aacatctgtt cacgcgtaag
    15001 ttggacgggc ctctgccggc gctggtggag gcggccgggg agaatgcgca gtctcgcttt
    15061 gtggcgcaaa aaatcctgga actgcgcgaa gaaggcgtgc cgctcagtga gattgccgtg
    15121 ctctttcgct ccagctttca ctccttcgat ctggaaattg agctgtcgcg ctgcggcttg
    15181 ccgttcgtga agcggggagg gatcaagttc attgaggcgg cgcatgtgaa ggatctcctg
    15241 gcccatttgc gggtggtcgt gaatcctcag gatgcggtca gttggcatcg cgtgctcatg
    15301 ctggtcgaag gggtcggtcc caagaaggcg caggatctgg tggcggccat ggtgagggtg
    15361 aacgatccgt atcaggtgct gtgggacagt agcggccgct cgggcaaggg gttgaaggag
    15421 ctggcgctgg tgctcgacaa cctgtcgaag agtgatgatc tgagcccgac cgagcaggtc
    15481 aaccgggtct atgagtatta tctgccgatc ctgaaagatc atcatgatga ctatcccaag
    15541 cggatccggg atctggacca tttgcatacc atcgcggaga gttaccccag cctggcagag
    15601 ttcctcgctg atttagcgct ggccccgccg gatggcagtg cggtgggtgt ggaacctgcg
    15661 gggcgcgacg acgagcaggt ggtgctgtcg acgattcatt cggcgaaggg gctggagtgg
    15721 cagtgcgtat ttttgttatg ggtggtggat gggaaattcc cctcggtctt ctcattcaat
    15781 acggatgaag agttggaaga ggagcggcgc ttattgtatg tcgcggtgac tcgggcgaag
    15841 cgctacctgt tcttgaccta tccgatcaat gtgtatgacc ggagttccgg catgttgtta
    15901 tccaagccat cgcgattcct ggatcacgtg tcgccccgtc tgtttgaaac gttggctctg
    15961 gtcgaagagg gccatgggca tgagtggggg cacgagcatg accggtacgt ctaggtcgat
    16021 cgttcccacc gtataactct ggttgggttg ccgtactcca ttctacgatt caagatcatc
    16081 atgcgtccgg tgctcgtcta taacggttgg ttccgagctg tgctgcccac gatggccgcg
    16141 gggctggtgc tgggacttct gatcttcggc caggccagcg cagagacggc cgcgccatct
    16201 tcggactctt cgcgcctctg gggcgcattg atggcggaag cgaaagccct ccatcttcct
    16261 actcggttcc tggaacaaat tccagcgcag tttgtggcgt ttgagtttga ggatctgcac
    16321 gcgtttgccg cggagtatca cccggtcgag catcgaatgg tgttggatcg atcgctctcc
    16381 ctgaatgcgg cggggaggac tctgcgtcca ttggcgcggt tgacgcacaa ggagttggaa
    16441 acgctctatc acgaactgtt tcatgcctac atggattttc tcgagcagga gcgtgcgccg
    16501 tcagccgccc acgcgtcgtt tatggcgttc gcccgcgagc agcaacggtg tcgctatcag
    16561 caggtcctca tcacgccggt gctccaaaag aaagatttga aagaggagcg ctttctcagc
    16621 gaggcggagt cgtgggaagc gttgaatgaa acctgggcgg tgtttgtggg atgggcgatc
    16681 tggacccaac tggaagtgga gaggaaggga gcgcgtgagc ttcgatcatc cggtcggtcg
    16741 ctgaacgtgc cggcttggat tgcccggctc agtcaggcgg agcaggacgc aagcctgaga
    16801 ggatattacg agccggagga tccaagtgaa aaggcgatgg cacgcaagcg atttctggcg
    16861 ccggactttc gcctgtctgc tccggaactc ttgttactca tgaaggaagt tttgggtagt
    16921 ccagcggaca tgactcgcca ggctgatcag gtattgacgc gcgggagggt tgttcctgcc
    16981 tcccgtggag cctgtgaggc tcctgccgca tcgtagattt gttcctgcat agaacatacc
    17041 cccttgacaa tggaacgcgt cgcaaataaa atcggctgct ttcgaagcca ggtcaatcct
    17101 catttcatca gcgctccagc cctgttgttt tgctggggag ctgtgcatgg tggggcttac
    17161 tttcattctt ctcttcaggg aatgaagtgg ctctggttcg ggatttattt gaaataaata
    17221 atattcgctt gagtttggga acgtgtgcct agcgggcagg tacccaagtg gacaaagggg
    17281 gcagactgta aatctgctgc cttatg
//
LOCUS       DAOQRM010000045        16906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2481_length_16906_cov_17.422052, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000045 DAOQRM010000000
VERSION     DAOQRM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16906
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2481_length_16906_cov_17.422052"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..467
                     /locus_tag="PKK81_05590"
     CDS             66..467
                     /locus_tag="PKK81_05590"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV32376.1"
                     /translation="MHDRTIRSAMDQSNNLSSARIELRQHQRCVVPPACLLSFAPFAP
                     TISFNGDAEGEGVVINLSAGGCKISSDAGVTVGDAMSLIILLPGELCPTTIDLALVRW
                     GRDLHFGVEFVSMGTTEFNRIRQFLASTGPE"
     gene            523..855
                     /locus_tag="PKK81_05595"
     CDS             523..855
                     /locus_tag="PKK81_05595"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV32377.1"
                     /translation="MEQTKTPRKSRRVAADCRLEFTGEDEVDGEAEVLDVSSTGCMAK
                     SDNRMSPGLHMKVALFLSDGHEWPVRVDEAVVRWARGGEFGVEFLAMRPAQLQRIQQF
                     VIKARPLS"
     gene            981..1856
                     /locus_tag="PKK81_05600"
     CDS             981..1856
                     /locus_tag="PKK81_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV32378.1"
                     /translation="MFPILPLQFRGIPHEYWLGPTVLLHGKGGSQRMIEWVGRKAIAG
                     YTYLAALATLFTQAVLDLVLPTRQGRGETLRVLVRQILFTGVDALPVTTVIALLLGII
                     IVTQAGTQLPRLGAGGLVGSIIVVTVIRELGPLITAFIVVGRSGTAIATELGNMSVTR
                     EVVALRLMGISISRFVIMPRMVGMVLSMLCLTLYFDVVAVLGGYLIADAQLTIPFYAF
                     VESITKALSTTDVLMTAIKGLSFGSAVAAICCYHGLAVRSSFTEVPQQTTRAMINSFV
                     LCLLIDVVVTVPLYL"
     gene            1853..2584
                     /locus_tag="PKK81_05605"
     CDS             1853..2584
                     /locus_tag="PKK81_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV32379.1"
                     /translation="MNEAALVLEQVRTPMAGSPHVLDVDLRIAAGEFVALVGPSRSGK
                     SLVIELAAGLVRPEAGRVVLLGHEWNDTSESEQTPVRLRVGTVLQQPGLLSNMTLFHN
                     VLLPLRYHHGAMPERSREQAVMAQLERLGVAPMRDRFPAELNQGEVRRAAIARALMLD
                     PEVLLLDDPVAGLDAEMVLALKACVEERRAHHPLTVVAALRAFSPFIEGADRLVVLRD
                     GRVEADGARDTVARTVPEMLRKYVE"
     gene            2631..>3550
                     /locus_tag="PKK81_05610"
     CDS             2631..>3550
                     /locus_tag="PKK81_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV32380.1"
                     /translation="MHYSHRLSSGRLAQIVGTFVLIPVLILAVAGFWMSKTEHLFEPK
                     YYLMASLSKSYGLEPGAPVVVSGIRVGRVKQVDLNDRGTVDLTLQLSARYQDMVKGNS
                     ELHITKSGVVVGQTQVEIGAGTASSPVLTDGAIIRTVEPRDIGDLLNEVEPVLMAVKQ
                     TLLRVETITQDLQGGLKAGGKALEQVAVATQDLPAVVASVQRTIASVEQTATALPAMT
                     GSVQRTLAVVDRVTADMQRTTSRLPAILDSAQSTLTSVKRLSDSVEEVSHELLPVVHT
                     AETTLADVSILVRGAKQTFPFNRFAQNAGPA"
     assembly_gap    3551..3650
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3772..4728
                     /locus_tag="PKK81_05615"
     CDS             3772..4728
                     /locus_tag="PKK81_05615"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32381.1"
                     /translation="MIVRGHGCSSGFWGVTLCLLLGCTVAPPPPPVPAPRPLSVQVES
                     LRLQGDRWMIRGDLARAGEAYEEARRLAESLDDMKGLVGALNDLGSVALGRGAGREAI
                     QLHGRAVSLAQQFGETDLLIAGLASLGAAEYQEGRTEEAGRRYQQALDLLQRAADQRT
                     EAMLRNNFGLIRQATGDVGQAEQLFRQAMALNQAAGHPAAEASNHVNLGILAEERREY
                     EVAEREFERALELDKTAERRAEIAADLLRLGRVAERRGFPDRGLAYSERAYRSYLAQG
                     NQAQAIAAITFALDCARRLGLVEEVARLEKELIRLTGSRSGR"
     gene            complement(4757..7069)
                     /locus_tag="PKK81_05620"
     CDS             complement(4757..7069)
                     /locus_tag="PKK81_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV32382.1"
                     /translation="MQPKPPLPGLGDATLKSGNAENVKRISGQILAAADVDQILLDLR
                     HEILSCFDAEDLTLFVVDSEKKEIFSKIPHLDTVQEVRTPITEQSLAGFCAKYLRPVN
                     VGDAYSPVELSAIHPSLTHDASYDKNTGFKTKQVLTYPVVAENKYLMGVIQLLNKKSG
                     GRFTRKDEECVAEIAKALGTAFYALRKTSKKPPSKFDYLLTNGKVSQHDLDNALAEAK
                     KGTTDIESLLIEKYKVPKAELGKSLANFHKCPYIEYNERTIVDIELLKNLNVDYLKKN
                     HWMPLKRDRAAIEILTDDPGDLDRVQDIKRTFPGLNIRFAVSLRRDISLFLASTTGTP
                     EITNKMNENVSDILGELVNESQLEAQEDASSAGLDENDNAIVRLANQIIADAFRQGTS
                     DIHIEPYGEKRDTLVRFRVDGDCFEYMKIPPSYRRAIVSRLKIMASLDIAERRKPQDG
                     KIKFKIGENKEIELRVATIPTAGYNEDVVMRILAASEPLPVDKMGFSERNLREIKSIA
                     EKPYGIILCVGPTGSGKTTTLHSVLGYINTPDIKIWTAEDPVEITQYGLRQVQVHSKI
                     GFTFAAAMRAFLRADPDVIMVGEMRDKETADTGIEASLTGHLVLSTLHTNSAVETITR
                     LLDMGCDSFSFADAMLGVLAQRLARRICKDCKEAYQPSKEEYEELRLGYGPDYWDTLG
                     VTFDANFRLYRGKGCDTCNRTGLKGRVALHELLLGTDQMKKLIQNKAKTDEMLKAGMA
                     EGMTTLVQDGIQKVMQGHTTYKEVKAVAIK"
     gene            complement(7282..8988)
                     /locus_tag="PKK81_05625"
     CDS             complement(7282..8988)
                     /locus_tag="PKK81_05625"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNV32383.1"
                     /translation="MRVSETLIPTLREDPGEAETVSHRLMLRAGLIRKVAAGIYTYLP
                     LGLRVLRKIERIVREEMNRAGAQELLMPVASPAELWRETGRWDFYGKELLRFKDRHER
                     DFCLGPTHEEVITDLFRREVRSYRQMPLNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
                     YSFDRDEAGARLNYQKMYDAYNRIFTRCGLTFRPVEADTGLIGGTSSHEFMVLAETGE
                     ETIVYSEDGTYAANVERAEVLPPDTADNSAHRPLTPVSTPNRRTVEEVTAFLKIEPRQ
                     LVKTLLYSTGKETVAVLVRGDHDVNEIKVKRLLGVPEIELLKPELVPGLTGAPVGFVG
                     PVGLKQVRIMGDWAVKAMANFVVGANQADTHFVDANWERDFKVDQFADLRNARAGDCS
                     PRKDGTLKTAKGIEVGHVFMLGTKYSQAMKATFLDAQGQEQLAVMGCYGIGVSRTAAA
                     SVEQNHDAKGIKWPVPIAPFHVTLLPLSQSQPVTQLAETLYRAMQEAGIEVLWDDRDE
                     RAGVKFNDADLIGAPYHLVIGEKGLAQGQVELKLRHTGETQKVAPDQVVSLLSSFLKA
                     AS"
     gene            complement(8995..10386)
                     /gene="rseP"
                     /locus_tag="PKK81_05630"
     CDS             complement(8995..10386)
                     /gene="rseP"
                     /locus_tag="PKK81_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNV32384.1"
                     /translation="MGTAFAWSPDVVSLLTHWALPFLVVLGVLVAFHEMGHFLAARWV
                     GVKVLKFSLGFGPKIFGRQIGETEYLLSVVPLGGYVKLFGEDEHETLTPEDKKRAFVH
                     QSLWGKTLIVAAGPIFNFILAYLIYTAYIGLGYTLPVPSFKDIIPEVEAVLPGSPADQ
                     AGLKPGDRVIRVNEKEISTNAELLKYIAQSNGKQLTLDLTRGEQVKTVLVTPSKTTVQ
                     DNGKATTIFQLGIEERAPVITAVIPGSRAQAAGLSAGDRVVRIDGHDIFTWSQMTSLV
                     RESPNRALQFDIQRGGTTQTLSVTPMGEKATVEGKPAEVGKIGISAQNQTILQTNDPL
                     KAPWLGAQATWGWTELTVVGIYKIITGDISRKNIGGPLTIAKTAGDAAEQGTSSLVFL
                     MAMLSINLGVLNLLPIPILDGGHLLFFFIEAIRRKPLEDRQRELAQQVGLVLLVGIMI
                     FAFWNDIERLISP"
     gene            complement(10412..11572)
                     /locus_tag="PKK81_05635"
     CDS             complement(10412..11572)
                     /locus_tag="PKK81_05635"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNV32385.1"
                     /translation="MKNIVILGSTGSIGTNTLDIVDRFPQEFRVVGLTAGSNDEKLEA
                     QIRKFRPAFAALANEAAAARLRDRCADLPVKILAGNEGVAEVAQSQEAELVISAIVGG
                     AGLLPTLAAIRAGKQIALANKEPMVMAGALMQAEAKKHHVRIFPIDSEHSAIFQSLEG
                     HRREDVKRIILTASGGPLWGFSREQLQDVSPERALQHPNWKMGSKITIDSATLMNKGL
                     EVIEARWLFDIPETQIEVLVHRESIIHSLVEYTDRSVIAQLGLPDMRTPISYAMRYPE
                     RMPLDLPSLDLTEISTLTFFKPDHARFPCLQLGYEALRIGGTMPATMNAANEVAVEAF
                     LQNGIRFLDIPEIIRSTMESHAPRPIDGLDDALEADRWARDKAESLVHALTR"
     gene            complement(11605..12453)
                     /locus_tag="PKK81_05640"
     CDS             complement(11605..12453)
                     /locus_tag="PKK81_05640"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNV32386.1"
                     /translation="MPPTLDRARPRSSSETVRRVLAAAVFLPIFYYLVHDLGAIAFFG
                     LAAIAGMLAAGEFYRLHLGQAPWPWWCWLGVGATGLLISSAQWPTLVTDRAVLLGTLA
                     IALCMPLLSGKSLRDSLTDGMVLVMGVLYLGLTLSYLVLTRGLPDGALLIFFVFLVTW
                     AGDTGAYVAGKSMGRHALAPVISPKKTYEGLAGGLVLACLMAVVARAWFLPAFSLVDC
                     LALGMILTITGLIGDLAESAMKRSAGFKDSGALIPGHGGMLDRLDSLLFTGPAFYYYV
                     TIVNNG"
     gene            complement(12497..13282)
                     /locus_tag="PKK81_05645"
     CDS             complement(12497..13282)
                     /locus_tag="PKK81_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNV32387.1"
                     /translation="MNDSRSRDIEPAPDSDLLSQLEPDLLPKHLAVIMDGNGRWAELR
                     GLPRIAGHQEGIKSVRELISLSLELGIKVLTIYAFSQENWNRPAQEITALMGLLEHYL
                     STERSSLVEKGVRFQTIGRVTALPPSALQWVRTTEQETAHLDKLILNVALSYGGRAEL
                     VDAARELARAVQSGRLSPDQIDEQAMQQALYTHHLPDPDLLIRTSGETRISNFLLWQL
                     AYTELYFTPTLWPDFRRRETLVALIEYQRRERRFGRVLSSVSS"
     gene            13475..14911
                     /gene="tldD"
                     /locus_tag="PKK81_05650"
     CDS             13475..14911
                     /gene="tldD"
                     /locus_tag="PKK81_05650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNV32388.1"
                     /translation="MSNDESGALARFGVTDREIEQALGRVKVSTVDYADLYFEYCQSE
                     SVSMEEGIVKRATKSVGQGVGVRATAGEKTGFAYSDELTPKDLNIAADTARYIANSAQ
                     GTAPVPVTHRPALARNLYPHDRANIEVATRDRVTLLNAIDAEARRYDPRIKNVMAAYN
                     TEYKVMLVATSDGLMVGDIQPLSRLQVTCIAEENGNRQVGTFGGGGRIGLEYFQNDQR
                     YLQFAREAAREAILNLSAVDAPAGVMPVVLGGGWPGILLHEAIGHGLEADFNRKKTSA
                     FSNLLGKKVASEVCTIVDDGTLPFRRGSLNVDDEGTPTSRTVLIERGILRGYITDKLN
                     ARLMGIPVTGNGRRESYQSVVLPRMTNTFMLAGESDPKDIIKSVKKGLYAVSFGGGQV
                     DITNGKFVFSASEAYLIEDGQITKPVKGATLIGSGPDILKQVSMVGHDLKLDEGIGTC
                     GKEGQSVPVGVGLPTIRIDEITVGGTKA"
     gene            14914..16272
                     /locus_tag="PKK81_05655"
     CDS             14914..16272
                     /locus_tag="PKK81_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNV32389.1"
                     /translation="MTRAIVEQDYTNIAQDLLARAAKHGATASDVMVADGETLSVQVR
                     MGAVDRLTKAREKRLGLRVFFGQRSASASTSDFSRESLERFVGETCALAQAVVEDPVS
                     GLPEPGQFATDFPELNIHDSTKLQTDQQIDLALRAERAAFAADSRITNSEGGECDSSS
                     GRIILANSHGFVGHYANSSFSLSVSPIASDAAGMQRDYWYGVNRAFAKLESPEAIGRE
                     ATRRTVRKLGARKVSTCRVPVIFDPEVAGSLLSHLCSALSGYALYKGASFLIGQLGQQ
                     IAPDFVTIYDDGRMPGGLGTRPFDGEGLPTRKQAMVERGRLASYILDTYSGKKLGLPS
                     TGNAARSIGESPSAGPTNFYMVPGTTSPEDILASVKQGLYVTDLIGFGINMVTGDYSR
                     GASGFWIENGELAYPVEEITIAGNLKQMYANIETIGTDLVFRGRIASPTVKLAEMTLA
                     GN"
     gene            16562..>16906
                     /locus_tag="PKK81_05660"
     CDS             16562..>16906
                     /locus_tag="PKK81_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNV32390.1"
                     /translation="MRRLMASMICALLVGPAVGRAAEFQVTSPTIKDQSTIGNEHVFN
                     GFGCTGGNLSPELRWDHAPKDTKSFAVTVYDPDAPTGSGWWHWLIFNIAPSVTHLPAG
                     AGQPESAGAPQGS"
BASE COUNT         3208 a         4709 c         5336 g         3553 t
ORIGIN      
        1 gcaaaatgat gagaagtatt cggcgtctcc cgctccttgc gatatactga tcctggctga
       61 atgcagtgca cgatcgcacc atccgctccg ccatggatca atccaacaac ctctcttccg
      121 cgcgcattga actccggcaa caccagcggt gtgttgtgcc cccggcatgc ctgctctcgt
      181 tcgcaccctt tgctccgacc atcagtttta acggtgatgc ggaaggtgaa ggcgtcgtca
      241 tcaacctgtc cgccggtggt tgcaaaatca gcagcgacgc gggtgtgact gtgggagatg
      301 cgatgtcgtt gatcatcctc cttccggggg aactgtgccc gaccaccata gatctggccc
      361 tggtgcgatg gggtcgggat ctacattttg gtgtggaatt tgtctccatg gggacaaccg
      421 aattcaatcg gatccgccag tttctcgcat ccaccggtcc cgaatagggc cggtatcggg
      481 gccgatgagg ctggggggtg gtgtgctgag aaaggagttt gtgtggaaca gacgaaaacg
      541 ccgcgaaaat ctcgtcgggt cgcagccgat tgccgattgg aatttaccgg ggaggatgag
      601 gtggatgggg aggcggaggt gttggatgtc tcctcgaccg gctgtatggc caagtcggat
      661 aacaggatgt caccgggact tcatatgaag gtggctttat ttctgtcgga cgggcatgaa
      721 tggccggtcc gagtggatga agccgtggtt cgatgggcgc gaggcgggga gttcggtgtg
      781 gagtttctgg cgatgcgccc tgcccaactc cagcgcatcc agcagttcgt catcaaggcc
      841 cggccgttgt cataggcctc gtacaacgtt cctccttcct gttgttgctc cgccgctatt
      901 tgtccacgct gaccagccca cacggcatct cctctcgcct gcgccggttc gagacgtcgc
      961 cggcagggcg actcattgct ttgtttccga tccttcccct acaatttcgc gggatcccac
     1021 atgaatattg gctcggcccg acggtgttgc tccatggaaa aggcggctca caacgcatga
     1081 tcgagtgggt ggggcgcaag gccatcgcgg ggtatacgta cctggcggcg ctggcgacac
     1141 tctttacgca agccgtgctg gacctcgtgc tccccacacg ccaaggccgt ggggagacgc
     1201 tgcgggtact cgtcaggcag attctgttca cgggggtcga tgcgttgccc gtgacgaccg
     1261 tcatcgcgct cctgctcggc atcatcatcg tcacgcaggc cgggacacag cttccgaggc
     1321 tcggggccgg tggcctggtg gggagcatca tcgtcgtgac cgtcattcgc gaactgggcc
     1381 cgttgattac ggcgttcatt gtcgtcgggc gctcaggaac ggccatcgcg actgagttgg
     1441 gcaacatgtc cgtgacacgc gaggtggtag cgctccggct catggggatt tccatcagcc
     1501 gatttgtcat tatgcctcgc atggtcggca tggtcctctc gatgctctgt ttgacgctgt
     1561 atttcgatgt ggtggctgtg ttggggggct atctcatcgc cgacgcgcaa ctgaccattc
     1621 cgttttatgc gttcgtcgag agcatcacga aggcgttgtc gacgacggat gttctgatga
     1681 ctgccatcaa aggattgtca ttcgggtcgg ccgtggcggc catctgttgt taccatggcc
     1741 tggctgtccg gtcctccttt accgaagtgc cgcagcaaac gacccgggcg atgatcaatt
     1801 catttgtcct ctgtctgctg atcgatgtcg tcgtcacggt gccgttgtac ctatgaatga
     1861 ggcagccctc gttctggagc aggtccggac gccgatggcg ggcagtcctc atgttctcga
     1921 tgtcgatctg aggattgccg cgggcgagtt cgtcgcgctg gtggggccga gccgctcggg
     1981 gaaaagcctg gtgatcgagc tggcagcggg gttggtgcga ccggaagccg gtcgggtggt
     2041 tctgctgggg catgagtgga acgatacctc ggagagtgag cagactcccg tgcggcttcg
     2101 agtggggact gtgctgcaac agccggggtt gttgagcaat atgaccctct ttcataacgt
     2161 gcttctgccg ctccggtacc atcatggagc gatgccggag cggagtcggg agcaagccgt
     2221 gatggcgcag ctggaacgac tgggcgtcgc gccgatgcga gaccggtttc ctgccgagct
     2281 caatcaaggc gaagtacgta gagcggccat tgcgcgggct ttgatgttgg accccgaggt
     2341 gctgctcctg gacgatcccg tggccggcct ggatgccgag atggtgttgg cgctcaaggc
     2401 ctgcgtcgaa gaacgtcggg ctcaccatcc gctgaccgtg gtcgcagcct tacgtgcctt
     2461 ttctccgttt atcgaggggg cggatcgtct ggtcgtgctt cgcgacggcc gcgtggaagc
     2521 cgatggggcc cgtgatacgg tggcccgcac cgttcctgag atgctcagaa agtatgtgga
     2581 atagaccgcg ttctgccgag gcggtccagc aagactgagg ggtggggggg atgcactatt
     2641 cacatcgctt atccagcggg cggttggctc agattgtcgg cacattcgtg ctgattcccg
     2701 tcctgatcct ggcggtggcc ggcttctgga tgtcgaagac ggaacatctc ttcgagccga
     2761 agtactactt gatggccagc ctcagcaagt cgtacggtct cgagccgggc gcgccggttg
     2821 tcgtatcggg gattcgggtc gggcgggtga agcaggtgga tctcaacgat cgcgggacgg
     2881 tcgatctgac gctgcaactg tcggcccgct accaggacat ggtgaagggc aattcggaac
     2941 tgcatattac caagagcggt gtcgtggtcg gccagaccca agtcgagatc ggggcgggta
     3001 ccgccagcag ccccgtgttg accgatggcg cgatcattcg cacggttgag ccgagagata
     3061 tcggggacct gctcaatgag gttgaaccgg tgctgatggc cgtgaagcag acactgctgc
     3121 gtgtcgaaac cattacgcag gatctgcagg gcgggttgaa agccggcggc aaagccctgg
     3181 agcaggtggc ggtggcgacg caggatttgc cggcggtcgt ggcatccgtc caacgcacca
     3241 tcgcctcggt cgagcaaacg gcgactgcgc ttcccgccat gaccggctca gttcagcgca
     3301 cgctggcggt ggtggatcgt gtcacggcag acatgcagcg gacgaccagt cgcttgccgg
     3361 ctatcttgga cagtgctcaa tccacactca ccagtgtgaa gcgcctctcc gattccgtgg
     3421 aggaggtgag ccatgagctg ctgccggtcg ttcacacggc ggaaactacg ctggcagacg
     3481 tctccatcct agtgcgtggc gcaaagcaga cctttccctt taaccgattc gcgcagaacg
     3541 ccgggccggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtggcgcaaa
     3661 gcagaccttt ccctttaacc gattcgcgca gaacgccggg ccggccccga ggccggaggc
     3721 cgccctgtca cccacggctc ccccgagttt gcgcggggat cacgtgcgtc gatgatcgtc
     3781 agagggcatg gttgttcctc aggcttctgg ggggtgacgt tatgtctcct gctcgggtgc
     3841 accgttgcgc cgccacctcc tcccgtcccc gcgcccaggc ctctaagcgt acaagtcgag
     3901 tccttgcgct tacaaggaga ccgttggatg atccggggcg acctggcacg tgccggggaa
     3961 gcctatgaag aagctcgccg gctggccgaa agtctggatg atatgaaagg actcgtcggg
     4021 gccttgaacg atcttgggtc tgttgcgctt ggccgtgggg ccgggcgtga ggcgatccag
     4081 cttcatgggc gggccgtctc cctggcgcaa caattcggag agacggacct cttgatcgct
     4141 ggtctcgcat cattgggggc ggcagaatat caagaggggc ggactgagga ggcaggccgg
     4201 cgctatcaac aggcgctgga tctccttcag cgggcagcag accagcgtac cgaagcgatg
     4261 ctccggaata atttcggact gatccggcag gcgaccggtg atgtcggaca ggcagaacaa
     4321 ttgttccggc aggcgatggc cctgaatcag gccgcggggc accccgctgc cgaggccagc
     4381 aatcatgtga acctggggat tctggcggag gagcgccgcg agtacgaagt cgcggaacgg
     4441 gaatttgagc gcgctctgga gttggacaag actgcggagc gccgggcgga gattgctgcc
     4501 gatcttctgc ggctaggccg ggttgcggaa cggcggggat ttcccgatcg tgggctggcc
     4561 tattccgagc gggcctaccg tagttatctg gcgcaaggga atcaggctca ggcgattgcg
     4621 gcgatcacct ttgcgttgga ctgtgcgagg agattggggc tggttgagga ggttgctcgg
     4681 ctggagaagg agctgatccg tctgaccggt tcccgttccg ggcgatagcg tacgctccgg
     4741 ttgtcgtccg ccgatgttac ttaatggcga cggctttgac ttctttgtag gtcgtgtggc
     4801 cttgcatgac tttctggatc ccgtcctgga ccagagtcgt cataccctcc gccatgccgg
     4861 ccttcagcat ttcgtcggtt ttcgctttgt tttgaatcaa cttcttcatt tgatctgtgc
     4921 ccagaagcag ttcgtgtagc gccacgcggc ccttcagccc tgtccgattg caggtgtcgc
     4981 agcccttgcc tcgatagagc ctgaagttgg cgtcgaatgt cacgcccagc gtgtcccagt
     5041 agtcagggcc atatcccaac cgcagttctt catactcttc cttcgagggc tgatacgctt
     5101 ccttgcagtc tttgcagatc cgtcgcgcca aacgttgggc gagcactccc agcatggcgt
     5161 cggcgaagct gaacgagtcg cagcccatgt cgagcaggcg ggtgatggtt tccaccgcgc
     5221 tgttggtgtg caaggtgctg aggaccaagt gaccggtcag cgaggcctcg atgcccgtgt
     5281 ccgcagtttc tttgtcccgc atttctccga ccatgatgac atccggatcg gcccggagaa
     5341 acgcgcgcat ggcggcagcg aacgtgaagc cgatcttgga atgcacctgc acctgacgaa
     5401 gtccgtattg tgtgatttcg acggggtctt ccgccgtcca gatcttgatg tccggcgtat
     5461 tgatgtaacc cagcaccgag tgcagcgtgg tggttttgcc tgatccggtc ggtcccacgc
     5521 agagaatgat gccgtacggc ttttccgcga tacttttgat ttcgcgcaga ttccgctccg
     5581 aaaatcccat cttgtccacc ggcagcggtt cgctcgcagc cagaatacgc atgaccacgt
     5641 cttcgttgta gccggcggtt gggatggtgg cgacacgcag ctcgatttcc ttgttctcgc
     5701 cgatcttgaa tttgatcttt ccgtcctgcg gttttcgccg ttccgcgatg tcgagactcg
     5761 ccatgatctt gagccgggag acgatggcgc ggcgatagct cggtggaatt ttcatgtact
     5821 cgaagcagtc gccgtcgacc cggaaccgca cgagggtatc gcgcttttca ccatagggtt
     5881 cgatgtgaat atcggaggtg ccttgccgga aagcgtcggc gatgatctga ttggccaggc
     5941 gcacgatggc gttgtcgttc tcgtcgagcc ctgcgctgga ggcgtcttcc tgagcctcaa
     6001 gttgggattc gttcaccagt tcgccgagaa tgtcggacac attctcattc atcttgttgg
     6061 tgatctccgg agtcccggtc gtgctcgcaa ggaagaggga gatgtcgcga cgaagactga
     6121 ccgcaaagcg gatgttcagg ccggggaagg tgcgtttgat gtcctggacg cgatccagat
     6181 cgccgggatc gtcggtgaga atttcaatcg ccgcgcggtc ccgcttgagc ggcatccagt
     6241 gatttttctt gaggtagtcg acattgaggt ttttgagcag ttcgatatcg acgattgtcc
     6301 gctcgttgta ttcgatgtag gggcatttgt gaaaattggc gagcgacttg ccgagttccg
     6361 ctttgggtac tttatatttt tcgatgagca gcgactcgat gtcggtggtc cccttttttg
     6421 cctccgcgag cgcattgtcc aaatcgtgtt gcgaaacttt tccgttggtc agcagatagt
     6481 cgaatttcga gggaggtttt ttcgacgtct tcctgagtgc gtaaaaggcc gtgcctaatg
     6541 ccttggcgat ctcggcgaca cattcctcgt ctttgcgcgt gaaccgtccg ccgctcttct
     6601 tgttgagcag ctggatgaca cccatgagat atttgttctc ggcgacgacg ggataggtga
     6661 gcacctgttt ggttttgaag cccgtgtttt tgtcgtagga ggcatcgtgg gtgagcgagg
     6721 ggtggatcgc actcagttcg accggactgt aggcgtcgcc gacattgacg gggcgaaggt
     6781 atttggcaca gaacccggca agactctgtt cggtgatcgg ggtgcggact tcctgaacgg
     6841 tatcgagatg gggaattttg gagaagatct ctttcttttc cgagtccacc acaaagagcg
     6901 tgagatcttc cgcgtcgaaa cagctcaaga tttcatggcg cagatccagc aagatctggt
     6961 cgacatcggc cgcggcgaga atttgcccgc tgatccgctt gacgttttcc gcgttgccgg
     7021 atttgagagt cgcgtcgcct agaccgggca gcgggggttt gggctgcatc aagttgtctc
     7081 caaaacgctt gcgcagaggg cggttgcggt gtcgaccggg atccgaacgc cgggcttctt
     7141 ccgtgtgagt cagtgtatcg caacgcgcag tcaaggcaag caaaagacac tttctcaaac
     7201 cgattctgcc ggacgtgtga ggacgtgtcc gcaggagtga agttctgtcg ggggagtgaa
     7261 cgattgaacc gtctgatgga gctaggaagc ggctttcagg aatgaggaca gcagggagac
     7321 cacctggtcg ggagcgactt tttgagtctc gccggtatga cgaagtttga gctcgacctg
     7381 accttgcgcc aaccctttct ccccgatgac caaatgatag ggtgcaccga tcagatcggc
     7441 gtcgttgaac ttgaccccgg cccgctcgtc acggtcgtcc cacaagactt cgataccggc
     7501 ctcctgcatg gcgcgataga gggtctcggc taactgcgtg actggttgag actggctgag
     7561 cggcaacagg gtgacatgga agggcgcgat gggaaccggc cacttgatgc ctttcgcatc
     7621 atgattctgc tcaaccgaag ccgcagcggt tcggctcacg ccgattccgt aacagcccat
     7681 gaccgccagc tgttcctgtc cctgcgcatc caggaaggtg gccttcatcg cctggctgta
     7741 ctttgtcccc aacataaaga catggccgac ttcgatgccc ttggccgtct tcagggtgcc
     7801 gtctttgcgg ggtgaacaat cgccggcgcg tgcattacgg agatcggcaa actgatccac
     7861 cttgaagtcg cgctcccagt tggcgtctac gaaatgggtg tcggcctggt tggcgccgac
     7921 gacgaagttg gccatggcct tcacggccca gtcgcccatg atgcggacct gcttcaatcc
     7981 caccggccca acgaatccga ccggcgcacc cgtgaggccg ggaaccagct cgggtttcag
     8041 cagctcaatc tccggcacac ccaagaggcg tttgaccttg atctcattca catcatgatc
     8101 gccgcggacc aggacggcga ccgtctcttt gcccgtgcta tagagcagcg tcttgaccag
     8161 ttgtcgtggc tcaattttca ggaaggcagt gacttcctcc acagttcgtc ggttcggggt
     8221 tgagaccggc gtaagcgggc ggtgagccga attgtcagcg gtatccggag ggaggacttc
     8281 ggcacgttcg acgttggcgg cgtacgtgcc gtcttcgcta tagacgatcg tctcctcgcc
     8341 ggtctccgcc agcaccatga actcatgtga cgaggtgccc ccgatcaacc cggtgtcggc
     8401 ttccacggga cggaaggtga ggccgcagcg cgtgaagatg cggttgtatg cgtcgtacat
     8461 tttttgataa ttcagtctgg ctccggcttc atcccggtcg aagctgtacg catctttcat
     8521 gatgaattcg cggccgcgca tcaacccgaa gcgtggacgg atctcgtcac ggaatttcgt
     8581 ctggatctga tagaaattca acggcatctg ccggtaggag cgaacttccc ggcggaagag
     8641 atcggtgatc acttcctcgt gtgtcggccc gaggcagaaa tcgcgctcgt gccggtcctt
     8701 gaaccggagc agctccttgc catagaagtc ccagcgtccg gtttctcgcc acagctccgc
     8761 cggagaggcg acgggcatga gcagttcctg cgctcccgcc cgattcatct cttcgcgaac
     8821 gatgcgttcg attttacgga gcactcgcag gcccagcggg agataggtgt agattccggc
     8881 cgcgaccttc cggatcaggc cggcgcgcag catgaggcga tggctgaccg tttcagcctc
     8941 gcctggatct tctcgcaatg tggggataag ggtttcggaa acgcgcatgt gggtttatgg
     9001 ggaaatcagc cgctcgatat cgttccagaa cgcaaaaatc atgatgccga ccaggagaac
     9061 cagtcccacc tgttgtgcca gttctcgctg ccgatcttca agtggttttc tacgaatggc
     9121 ctcaataaaa aagaacagga ggtgcccgcc gtccagaatg gggatgggga gaagattcag
     9181 cacgcccagg ttgatgctga gcatcgccat caggaacacc aggctggagg tgccctgttc
     9241 agcggcgtcc cctgcggttt tggcgatcgt gagcggcccg ccgatgttct tgcgggagat
     9301 gtcccccgtg atgattttgt agatgcccac caccgtcagt tcggtccatc cccaggtggc
     9361 ctgggcgccc agccacgggg ctttgagcgg atcgttcgtc tggagaatag tttggttttg
     9421 ggccgaaatg ccgattttcc cgacttcggc cggctttccc tctacggtgg ccttttcgcc
     9481 catcggcgtg acggagagcg tttgggtggt gccgccccgt tgaatatcaa actgaagcgc
     9541 gcgattcggg ctttcccgca cgagggaggt catctgcgac caggtgaaga tgtcgtgtcc
     9601 gtcgatgcgg acgacccggt cgccagccga taggcctgca gcctgtgcgc gagaccccgg
     9661 gatcacggcc gtgatgaccg gcgcccgctc ttcaatgcca agctggaaga tggtcgtggc
     9721 tttgccgttg tcctgcacgg tcgttttgct tggtgtgacc agcaccgtct tgacctgttc
     9781 cccgcgcgtc aggtccaacg tgagttgttt cccgttgctt tgagcgatgt atttcaggag
     9841 ttcggcgttg gtcgagattt ctttttcgtt cacccggatg acccggtcgc caggcttcag
     9901 gccggcttgg tcggcaggtg atccagggag tacggcttcg acttcaggaa tgatgtcctt
     9961 gaagcttggg accggcagcg tatagcccag tccgatatag gcggtgtaaa tgagataggc
    10021 gaggataaag ttgaagatcg gaccggccgc gacgatgagc gtcttgcccc acagcgattg
    10081 atggacaaac gcccgtttct tatcttccgg cgttaaggtt tcgtgctcgt cttcgccgaa
    10141 gagcttcaca tatccgccca ggggcacgac cgacaacaga tattctgttt ccccgatctg
    10201 gcgcccgaaa atctttggtc caaacccgag cgaaaacttc aggactttca ctccgaccca
    10261 gcgcgcagcg agaaaatgtc ccatttcgtg aaaggcgacg aggacgccca acaccaccag
    10321 gaacggaagc gcccagtggg tcagaagcga gaccacatca ggagaccagg caaaggctgt
    10381 tcccacaaca acactccttg ggttcagggt attaccgcgt caaggcatgc acaagagact
    10441 cggctttgtc gcgagcccag cgatcggctt ccagcgcatc gtcgaggccg tcgatcggac
    10501 gcggcgcatg agactccatt gtactccgaa taatctccgg aatatcgaga aaacgaattc
    10561 cgttctgaag gaatgcttcc acggccactt cgttggcggc attcatcgtg gccggcatgg
    10621 ttccgccgat ccgtaacgcc tcataaccga gctgcaaaca ggggaatcga gcatggtcgg
    10681 gcttgaaaaa cgttaaggtg ctgatctccg tcagatcgag cgagggaagg tctaacggca
    10741 tccgttcagg ataccgcatg gcataagaaa tcggtgttcg catatccgga aggcccaact
    10801 gcgcaatcac cgaccggtct gtgtattcta ccagtgaatg gatgatgctc tcgcgatgca
    10861 ccaggacctc gatctgcgtc tccggaatat cgaagagcca gcgcgcctca atgacttcca
    10921 gtcctttgtt catcagtgtg gccgagtcga tcgtgatctt ggagcccatt ttccagttcg
    10981 ggtgctgcag ggcgcgctcg ggactgacgt cttgaagttg ctcgcgggag aagccccaga
    11041 gcgggccgcc ggaggcggtg agaatgatac gtttgacgtc ctcgcgccgg tgtccttcga
    11101 gcgactggaa gatggcgctg tgttcgctgt cgatggggaa aatgcggacg tgatgtttct
    11161 tggcttctgc ctgcatcaat gcgccggcca tgaccatcgg ctctttgttg gcgagcgcga
    11221 tctgtttgcc ggctcgaatg gccgccagcg tcgggagcag ccctgcgccg ccgacgatgg
    11281 cggaaatgac caattcagct tcctgcgact gcgccacttc ggccacgcct tcgttgcccg
    11341 caaggatctt gacggggagg tcggcgcatc gatcacgcaa tcttgccgcc gccgcctcgt
    11401 tggcgagcgc cgcgaaggca ggacggaact tgcggatctg cgcctcgagt ttctcgtcgt
    11461 tcgagccggc ggtcaggccg accacgcgaa actcttgcgg aaaccggtcc acgatatcga
    11521 gcgtgttcgt tccgatcgag ccggtcgaac caagaatgac gatgtttttc atagcgtgct
    11581 ccagaacggg gggacaatca tctgtcaccc gttgttgacg atcgtgacat agtagtaaaa
    11641 ggccgggccg gtgaacaaga ggctatccag gcgatccagc atacctccat ggcccgggat
    11701 cagggccccc gagtctttga atcccgcact gcgcttcatg gccgattccg ccaggtcgcc
    11761 gatgaggccg gtgatggtga ggatcatccc aagcgccagg caatccacca gcgagaaagc
    11821 gggtaggaac caggcacgcg cgacgacggc catcaggcag gcgaggacga ggccacctgc
    11881 caatccctcg taggttttct tcgggctgat gaccggcgcc agcgcgtgcc gtcccatgga
    11941 ctttcccgcc acataggcgc cggtatcacc ggcccacgtg accaggaaga cgaagaaaat
    12001 cagcagggcg ccgtcgggca accctctcgt gagcacaaga tagctgagcg tgaggccgag
    12061 atagagcacg cccatgacca gcaccatgcc gtccgtgagt gagtcgcgca gcgactttcc
    12121 tgagagcagg ggcatgcaca gcgcaatcgc cagggtcccg agcagtaccg cacggtcggt
    12181 gacgagggtc ggccactgcg cgctgctgat caaaagtccg gtcgccccga cgcccagcca
    12241 gcaccaccat ggccaagggg cctgtcccaa atggagccga taaaactctc cggctgcgag
    12301 catgccggca atcgcggcca ggccgaagaa cgcgatcgcg ccaaggtcgt ggacgaggta
    12361 ataaaagatc ggcagaaaca ccgctgccgc cagtacgcga cgaacggttt cggatgaaga
    12421 tcgtggtcga gccctgtcca gtgtgggcgg catagttttc tcgcactgaa tagctcctgg
    12481 tttcaccacg gagaagctag gaggagacgc tgctgagcac ccggccgaag cggcgttcgc
    12541 gccgctggta ttcgatcagc gcgaccaacg tttcgcgccg ccggaaatca ggccagagtg
    12601 tcggggtgaa gtacagttcc gtataggcca gttgccacaa caggaaattg ctgatgcgcg
    12661 tttcgccgct ggtgcggatc aggaggtccg gatcgggcag gtgatgggtg tacagagcct
    12721 gttgcatggc ctgttcatcg atctgatccg gtgaaagccg gccgctctgc accgcccggg
    12781 cgagttctct cgcggcatcg accagttccg cacggccacc atagctcaag gccacattga
    12841 ggatcagttt gtccaagtgg gcggtttcct gctccgtcgt ccggacccat tgtaaagcgg
    12901 acgggggcag ggccgtgacc cggccaatgg tctgaaagcg cacgcctttt tcaaccaggc
    12961 tggatcgttc tgtggacaaa taatgttcca gtaggcccat caacgcggtg atttcctggg
    13021 cgggccggtt ccaattttcc tgcgaaaacg cataaatcgt gagcaccttg atccccagtt
    13081 ccagagaaag agagatcagt tcccgcacgg acttgatgcc ctcctggtgc ccggcaatac
    13141 gcgggaggcc ccgtagctcg gcccagcggc cgttgccgtc cataatcacg gccagatgtt
    13201 tcggcagcag gtccggttcg agttgcgaga gcagatcaga gtcgggggca ggctcgatat
    13261 cgcgtgatcg agaatcgttc atggaacggc gtgagcacct ttttttagac tatgtggatc
    13321 ctcggggaag taaggcgagt ctagcggtgg ggtccaggaa agtcaaacag tatttcccaa
    13381 tttcgctgta tttgcagatg gttcgagcgt gtttgcattg cgcctccgga aaagagtgcg
    13441 ttatactcac gcaccgtacg agaggagcac atgcatgtcc aatgacgagt ccggcgcact
    13501 ggcgcgtttc ggggtaaccg atcgagaaat cgagcaggcg ctgggacggg tcaaggtctc
    13561 gaccgtcgac tatgcggacc tgtattttga gtattgccag tccgagtcgg tctccatgga
    13621 agaggggatc gtcaagcggg ccacgaagag tgtcgggcag ggcgtcggcg tccgggcaac
    13681 cgccggcgaa aaaacaggct ttgcctattc cgacgaactg acccccaaag atttgaacat
    13741 tgccgccgat acggcgcggt atatcgccaa tagcgcccag ggaacggcac cggttccggt
    13801 cacccaccgc cccgccctcg cacgcaatct ctatccccat gaccgggcca atattgaagt
    13861 cgccacccgc gatcgggtca cgctgctcaa cgcgatcgat gccgaagcca ggcggtacga
    13921 tccccggatc aagaacgtca tggcggccta taacaccgaa tacaaggtca tgctggtggc
    13981 gacgtccgac ggtctgatgg tcggcgatat ccaacccttg tcgaggctcc aggtcacctg
    14041 tattgccgaa gagaacggca atcggcaggt ggggacgttc ggcggcggag gacgaatcgg
    14101 actggagtat ttccagaacg accagcgata tctgcagttc gccagagaag cggcgcggga
    14161 agccatcctg aacttgagcg cagtcgatgc gccggccggt gtcatgccgg tggtgttggg
    14221 cgggggatgg ccggggatcc tgctgcacga agcgatcgga catgggctgg aggccgattt
    14281 taaccggaag aaaacctccg cgttctcgaa tctgctggga aagaaagtcg cttcagaggt
    14341 ctgcacgatc gtggacgacg ggacccttcc cttccggcgc ggctcgctga atgtggacga
    14401 cgagggcacg cccaccagcc gcaccgtctt gatcgaacgc ggtattttgc gcggatacat
    14461 caccgacaaa ttgaatgccc gcctgatggg tattccggtg accggtaacg ggcggcgtga
    14521 gagttatcaa agtgtcgtgt tgccgcggat gacgaatacc ttcatgctgg ccggagaatc
    14581 agatccgaag gacatcatca agtcggtcaa aaaggggctg tacgccgtct cgttcggcgg
    14641 cgggcaggtc gatatcacca acgggaagtt cgtgttctcc gccagcgagg cctacctcat
    14701 tgaagacggg cagatcacca agccggtgaa aggtgcaacc ctgatcggca gcggaccgga
    14761 tattctcaag caggtctcga tggtggggca cgatttgaag ctcgacgaag gaatcggaac
    14821 ctgcggaaaa gaagggcagt cggtgccggt cggcgtcggc ctgccgacca ttcgaattga
    14881 cgaaatcacc gtcggcggga cgaaagcata accatgacac gtgcgattgt ggaacaggac
    14941 tatacgaata tcgcgcagga tctgctggcg cgtgcggcga aacatggcgc gacggcgtcg
    15001 gacgtgatgg tggccgacgg ggaaaccctg tcggtgcagg tccggatggg cgccgtcgat
    15061 cgactcacga aggcacgtga aaagcggctc ggcctgcggg tctttttcgg tcaacggtcc
    15121 gccagcgcat cgacttccga cttttcccgc gagtccctgg aacggttcgt cggtgaaacc
    15181 tgtgccttgg cgcaagcggt cgtcgaggat ccggtgtccg gccttccgga gccgggccag
    15241 ttcgccaccg atttccctga gttgaatatt cacgattcca cgaaacttca gacggatcag
    15301 cagatcgacc tggcgctgcg tgccgagcgg gctgcgtttg ccgccgattc gcgtattacg
    15361 aattccgaag ggggcgagtg cgactcgtca tccggccgga tcatcctggc aaacagccac
    15421 gggtttgtgg ggcactatgc caacagtagt ttctccctat ccgtctcgcc gattgcgtcg
    15481 gatgcggccg ggatgcagcg ggattactgg tacggggtga atcgtgcgtt tgccaaactg
    15541 gagagtccgg aagccatcgg ccgggaagcg acgaggcgga cggtccggaa gctcggcgcg
    15601 cgcaaagtct ctacctgccg tgtgccggtg atctttgacc ctgaagtggc ggggagttta
    15661 ctcagtcacc tctgtagtgc cctctcaggc tatgccctgt ataaaggcgc atccttcctg
    15721 atcggacaac tgggccagca gatcgctccg gactttgtca ccatttatga cgacggacgg
    15781 atgcccggcg gcttggggac gcgtccattc gatggcgagg ggctgccgac caggaagcag
    15841 gcgatggtcg agcgtggtcg tctggccagt tatatcctgg acacctattc aggcaagaag
    15901 ttaggactgc cttccaccgg aaatgccgcg cgaagcatcg gagaaagtcc ctccgccggg
    15961 ccgacgaatt tttatatggt cccgggcacc accagtccgg aggatatctt ggcgtctgtg
    16021 aagcaaggtc tctatgtgac ggatctgatc gggttcggca tcaatatggt taccggcgac
    16081 tattcccgcg gggcgagcgg gttctggatt gagaacggcg agttggccta cccggtggaa
    16141 gaaattacca tcgccggtaa tctgaagcag atgtatgcga atatcgagac gatcggcacg
    16201 gatctcgtgt ttcgcggacg gattgccagc cctaccgtga agctcgcgga gatgacgctg
    16261 gcagggaact agcgggctgc ggaagcactt gactgttgcg caatacgcca cgtatcaact
    16321 catgcgccgt gttggtattg caataacccg caggatgtga aaaaaggccg tccagcaagg
    16381 ccgcagcaag cgaggcggcg aatcgtactc gcgctgtacg gtgagcttct gaggttcacg
    16441 acgcgcggaa taacgcgcgt cacgtttgtg aacgccgccg agacgtgagg cggcagtgtc
    16501 ttgcgagaac gccgctggcg gactttttcc gcatcctgct aggttgcgaa gggggtaagt
    16561 gatgcgacga ctgatggcct cgatgatctg tgccttgctg gtgggtcctg cggtcgggcg
    16621 ggcagcggaa tttcaggtaa cgagcccgac gatcaaggat cagagcacga tcggcaatga
    16681 gcacgtgttt aacgggttcg gctgcacggg tggcaacctg tcgcctgaac tgcgatggga
    16741 tcatgcgccg aaagacacga agagttttgc tgtgacagtc tatgatccgg atgcccccac
    16801 gggaagcggt tggtggcact ggctcatctt caatatagct ccgagcgtga cgcacttgcc
    16861 ggccggagcg gggcagccgg agagtgccgg cgcgcctcaa gggagc
//
LOCUS       DAOQRM010000046        16515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2592_length_16515_cov_18.029708, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000046 DAOQRM010000000
VERSION     DAOQRM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16515
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2592_length_16515_cov_18.029708"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="PKK81_05665"
     CDS             complement(<1..1301)
                     /locus_tag="PKK81_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32391.1"
                     /translation="MASGRSSEQLCDLLAEQLGPDAAAAVVKAIADSAARPDALDGVL
                     VLLDELTEEAPKAAKAAIDALADMQRRGLLADVLPWVDLGVAIAADSGALALRFFKES
                     PLLLGLLEPSSRAIVLAAGLELADRQANVAVEFIRIAPEAVGVLSPADWPVWMDLACE
                     LTETDFALAVEYIRQIPSIARVLSLDAVRAWVRFGMKLIVENSLGKPDYLGVLEFFRT
                     SPAILEDIPGPSHRNQVIGIGAQMAERSPASGIAVLAEAPAMLRRLLSEEWRTQVLRY
                     GLLVAERDAETALAYLRRCPELIALIGSGEGAQEKFQSWFASGMEVLEYSLEGGRAYF
                     AMETEKALGAVSQAMSGVPLRQIARRVKLFAQALCGRDVRIHDLPDSLESTQPMARAT
                     VSEDGQSIGLPSLVRRYPTYDENVRLYMVMAAHEAGHLEFGT"
     gene            1439..1885
                     /locus_tag="PKK81_05670"
     CDS             1439..1885
                     /locus_tag="PKK81_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HNV32392.1"
                     /translation="MAEPSKESLDKMWKYVKGFAEKSGTAMHPNEAVTNAVVLGLAAH
                     VDELGKPLCPCNFYPDKQAEAKLRRWMCACDEMQMYKYCHCLLFVREDGMPITEYLPE
                     DHEGRQCYGLITDPTPEKGRALRHKALPMAPKETTPPTTPESAPQP"
     gene            1894..3129
                     /locus_tag="PKK81_05675"
     CDS             1894..3129
                     /locus_tag="PKK81_05675"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNV32393.1"
                     /translation="MPPGRNPAVALMLSALSLLLGLATTGCLRTMEAIDPRSLPITST
                     SGQRLQHHVSFLASPELSGRQPGSPGNRQAARYIEDQFRAVGLEPLPSLGGYRQAISP
                     TIGDNLLGFIPPTAGSAPAQWMVIGAHYDHLGYPFLGADDNASSVAILIETARHMAGL
                     PQHGILFVAFNSEESPYFGKAEMGSQFLFHHLPPEIGRAENLQAVVIMDLMGGVQWPP
                     IKDAIFAAGAEKSPALYQRVKQASAPSLTVFPVGMHLVEEIPEIGHKAFSDYDVFRNH
                     GLPFLFLSAGRTPRYHTAGDVTSTLHYDRMAATVEWLRHLIALIGQDEAPYGFQADRV
                     EWADEVVSFREIVNRAVQDETRIPGTSRWSLRKLRQDAEWLRDADRSAPTPQDRDRLE
                     RISIRVQCLMADYSGCFTF"
     gene            complement(3170..3604)
                     /locus_tag="PKK81_05680"
     CDS             complement(3170..3604)
                     /locus_tag="PKK81_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32394.1"
                     /translation="MKASRSSFCLIFLVTIGLVACAGPEPILKSNTRVQLYGKDQAAR
                     DAAVCGEKAERAGLQHGTNRSGNAGAGATLGIVGGAAVGASAGLIGGPTGIAIGAAAG
                     GAVGGVLGFLAGTYKPLQPQPDYAAFVERCLKEKGYETTGWQ"
     gene            complement(3752..4069)
                     /locus_tag="PKK81_05685"
     CDS             complement(3752..4069)
                     /locus_tag="PKK81_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNV32395.1"
                     /translation="MRERQATEREQAVRATSEGVTISVHVQPKASRSECAGLHGHAVK
                     IRIAAPPADGAANDELCRFLARLCDVPLSAVHILSGAGSRQKRVLVKGRSVEQVRAQL
                     HLE"
     gene            complement(4135..4974)
                     /locus_tag="PKK81_05690"
     CDS             complement(4135..4974)
                     /locus_tag="PKK81_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNV32396.1"
                     /translation="MSEGRTASVPKGQEQEIDRYRIAREPFYAEVRGEVGLFTIAAQS
                     RMPVMLKGPTGCGKTRFVQHMAYRLGRPLITVACHEDLTASDLVGRYLLKGQETVWMD
                     GPLTLGVKQGAIVYLDEIVEARKDTTVIIHPLSDDRRLLPIEKKGQVIEAVDDFMLVI
                     SYNPGYQSILKDLKQSTKQRFMAIEFDYPLPDVESRVVAHEAGVSLEVAQRLVKIAEK
                     VRNLKNHGLEEGVSTRLLIYAATLIRQGVPADQACDVAIARPITDDPDMLRSIIEVVK
                     AIF"
     gene            complement(5095..8196)
                     /locus_tag="PKK81_05695"
     CDS             complement(5095..8196)
                     /locus_tag="PKK81_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV32397.1"
                     /translation="MAASLPAGSPRRSYPWLPVLIVGVTIVALVIGVVMLRSIEVRMV
                     EATGENLTLASAEIADKLDRLLFERHADVRMMARAFSDRALDKKYINEYLQWMKETYA
                     PVYLWLGVTDAHGRMVAATDSSVIGQDYSRSGWFDRVRQTGTVQVDEVEIHESNHKIE
                     SVAFTAPILSVGGQFLGAVTTRIGLPMLEDVLIETVGEIQQTDGSAGPFEYQFLTKAG
                     VVFVDSASSGIDRVNLTELGLPSVLLSRSGKAGYVEEIHVRRHVPVVTGYSQTKGYSD
                     YSGLQWTILLRMDRDLILLPIRAMMWKLGMAGVVVWLPMVGLLLWATGRLREQHRQAQ
                     QESEWARAAEAALLQSQERNRVIVDTALDAVISMDAGGIITDWNAQAVNIFGWEREEA
                     LGRRVSETVIPVRDRDEHERGLRHFLDTGQGALLNRRIEMMGSHRDGHEFPVEITISP
                     ARLGDAYIFSAFIRDITARKRTERQLASQYAVTRVLAESRTLEEAGPKILQAICESLE
                     WELGIFWRLDRQGSVLRCLDVWQSPALNAEEFVLDTWRHTFTIGKGLPGRIWQAGEPT
                     WIKDVVQEANFPRGGAAARVGLHGAFGFPVRVGTEVEGVIELFRREIKQPDEQLLKMV
                     ADVGLKIGQFGERARAEDALRRTEAQLQQSQKMEAVGRLAGGVAHDFNNMLTVIRGYS
                     ELVLSRLAPTDGFRKELEEVKKAADRASGLTGQLLAFSRRQFIAPKVLDLNSVIHNME
                     GMLRRLLGEDIVELCTVLDPGLGQLKADPGQVEQVIMNLAVNARDAMPNGGRLTVETS
                     NVQFGQRRNRPPMGAEPGSYVALIVRDTGHGMDEDTQSHVFEPFFTTKEKGKGTGLGL
                     STVYGIVKQSGGCIEVESKPGRGATFKIYFPRVESATTETESVAVGGDSVRGRETVLL
                     VEDEPGVRRLVNETLRLHGYTVLEARHGIEALLTGAKHAGPIHLLLTDVVMPQMSGPE
                     VAEKLATVRPEVKVLYMSGYPDHPAFSKGGIDTEHSFLQKPFTPTTLAQKVREVLDGS
                     KVA"
     gene            complement(8199..9629)
                     /locus_tag="PKK81_05700"
     CDS             complement(8199..9629)
                     /locus_tag="PKK81_05700"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNV32398.1"
                     /translation="MPSSACLSCDVCCRFPERDSFLRPFFTADEIETAIAAGLAPALF
                     PNPDGSQIDLVPNPEGDGYVCPAFDCATSRCRIYEVRPLDCRLYPFALMWDARHEQVL
                     LGWDTKCPYMRDQSSPLVAQAADEIAGWIEQDARVETLVRYPRLIGRFQEDVIVLRPL
                     ERVTERLQQGRMRVRTQAFTLGDRGRMDAALAVSAGVQGTPLAAASFAYHYIWRHRVT
                     YSWAVLHDHLCLFADCPDGLFMALPPLGPGPLAQPLAEALRFMRERNGDSRVTRVENV
                     PEACVDEVRALGYRATAKEPDYLYDASALSDLSGESFKSPRAACNRFIRERGGMLEPY
                     EPRDERACVSLFHEWREQKQQAGSDDWACALLEDAAGAHETALSDAAELGLIGAVVRV
                     GGRIRAYTMGLWLNPSVFCVLLEVADRDIVGLGPFVFREFCRQARAKGACWINTMDDS
                     GLPSLARSKQWYHPARLLPNYIVTES"
     gene            9929..11443
                     /locus_tag="PKK81_05705"
     CDS             9929..11443
                     /locus_tag="PKK81_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32399.1"
                     /translation="MALAQHSREPLNSDEARRRCLAIRDVLVRAGVYGPAETGDGAAT
                     TDSSIPTPRFRLAPTPFPLSSSDVTFFQELGQDLLSFYRALNRLYQDSTRGTQPAWVA
                     AYLDQGKPESLITYSRMNRFRTLIPGIIRPDVIPTADGMVITELDSVPGGIGLTGCLT
                     RAYDDQFTIERQPPGLPPERSPLIAGRDGMLQGFAAMLRAQQGDQEGCLAIVVSDEAK
                     EYRAEMEWMAQRLREIGYQAFCVEPRAVHFSEEGLFLQEETGPRPIGLLYRFFELFDL
                     KNIPKAELVMYAAKKGQVTVTPPYKPALEEKLAFGLFHHPELAPFWEQALRPETAINL
                     RRLLPRTWILDPQPVPPSAVIPHLTISGRAVSDFRRLAQTTQKERQLVLKPSGFSELA
                     WGSRGVSIGHDLPQVEWATALEQALQAFPTTPYVLQEFHKGRLFELSYFDERSDTVVP
                     MSGRVRLSPYYFVVGDKAELGGILATLCPANKKIIHGMVDAIMAPCAIAPNLAS"
     gene            11519..11764
                     /locus_tag="PKK81_05710"
     CDS             11519..11764
                     /locus_tag="PKK81_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNV32400.1"
                     /translation="MALTLYHVQWCPDCAVVRDRLDELKLSYEDVVVPDFRPMRTQVF
                     EVSGQYYVPVLKDGDTVLTETHDILAHLHTQYDKARP"
     gene            12074..13207
                     /locus_tag="PKK81_05715"
     CDS             12074..13207
                     /locus_tag="PKK81_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNV32401.1"
                     /translation="MEDWRRILAQSVVKPKDLADRLGVDPKEIEDIVGDYPMRITPTV
                     LATIKEKGDAIWKQVVPDRAEMADADAEDDPLEEDLMSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKKGELDRAIAYLRDHQEVRDVILSGGDPLLLPDHLLER
                     ILKSLRTIPHLELIRIGTRVPGSLPERITPKLCEIIKKYHPFYMNLHFNHPDELTPEV
                     KRACGMLADAGVPLGAQTVLLKGVNDDPEIMKRLMHQLLLARVKPYYLYQADLTKGTN
                     HFRTSVETGLKIIKSLQGHTSGMGVPHFVIDAPGGGGKIPLLPADYLVNLDEDSAVLR
                     NYENRTFHYPQPGSAPGRELPMVGAHPSWASTGNGCDGGGEHL"
     gene            13204..14355
                     /locus_tag="PKK81_05720"
     CDS             13204..14355
                     /locus_tag="PKK81_05720"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HNV32402.1"
                     /translation="MTRAASSKGILPYQHITQLIARESITSDHPIEDRQIQPASLDLR
                     LGKKAYRLISSFLPELSAISSRLNALDFYQSDLVMYEMDLTQGAILEKGHVYLVPLLE
                     QLDLPATLRARANPKSTTGRLDVFTRVVTDLNAGFDEIRAGYRGPLYLEVVPRSFAIK
                     VRTGDSLNQIRFVRGDATVSDSALQKLHGTDPLLFRNASPPKPLPQKEFRTERGLFLR
                     IDLTGSAREDAVIGYRAKKNSHVIDLAKIGHYAALDFWEPLKRHRQDSLLLEPEEFYI
                     LASKERIRVPPGYAAEMVAYEAACGELRTHYAGFFDPGFGYGDGKMRGTQVVLEVRPH
                     DVPFLIHDGQTFFKVVYDRMLTVPTQVYGTALGSSYQRQALTLSKHFKA"
     gene            14358..14765
                     /locus_tag="PKK81_05725"
     CDS             14358..14765
                     /locus_tag="PKK81_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32403.1"
                     /translation="MAPPSDLPDPSTRPSRPPLHRPDDESNVDRQTHVVGMMVGTALM
                     FIGFLDVFLSISGGFEINVVPLLIYFSGIAVWANAVVENPTIRYSLMAGSVLISLAFF
                     HYGEVLFWHKQVVFWSTVVVVMYFMFNEPKQAT"
     gene            14831..15559
                     /locus_tag="PKK81_05730"
     CDS             14831..15559
                     /locus_tag="PKK81_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNV32404.1"
                     /translation="MTIAVIIPVLNEACGIGQTLSHAATLGFDELIIVDGGSSDQTCA
                     VVESCAGRLQNRPCSGPAPASPAIRLLKAAAGRARQLNAGAAGSRCEVLLFLHADTYL
                     PTNARQAISTALSDEACVGGRFDVRFDSPRPIARLVGHMMNLRSRWSGIATGDQALFV
                     RRDVFERTGRFAEIPLMEDIEFSRRLKRAGRLAPLRSQVVTAFRRWERNGPVRTILLM
                     WTLRFLYWIGVSPYRLQHFYSIVR"
     gene            15564..16316
                     /locus_tag="PKK81_05735"
     CDS             15564..16316
                     /locus_tag="PKK81_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNV32405.1"
                     /translation="MSAPPSDRKQPASAALVIFAKAPIPGQVKTRLCPGLTEDEAATL
                     HGSFVLDTLERTKAAVHKFKLPVDRFLACAPSSTHVFFRIMEARHGVTLLDQPGDNLG
                     DRMAQAFSTLFARGYRHVFLVGTDVPSLPLTHYRDAVQSLTRHDLILGPAKDGGYYLI
                     GMTAPHPELFADIPWSTDQVMGLTQQKAAAAGLKTALLPAWSDVDTLDDLHALIDDCA
                     ADKKRPKQERVYSQRTAGTLELLAKRLRTREA"
     gene            complement(16332..>16515)
                     /locus_tag="PKK81_05740"
     CDS             complement(16332..>16515)
                     /locus_tag="PKK81_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32406.1"
                     /translation="AVEKVRQRRSRIAPRLTVRHEYDSPLRLLRPCWTAFLNSLRGIP
                     VLSVHEGHNSIFGVVI"
BASE COUNT         3356 a         5419 c         4623 g         3117 t
ORIGIN      
        1 gtgccgaact ccagatgtcc ggcctcatgc gccgccatga ccatatagag acgcacgttt
       61 tcatcgtagg ttggataccg cctgacgagg gacggcaggc cgatgctctg cccgtcctca
      121 ctcaccgtgg cacgagccat cggctgggtc gattcgagag agtcggggag atcgtgaatg
      181 cgcacatcgc gaccacagag cgcctgcgcg aacaatttga cgcgccgggc gatctgccgc
      241 agcggcacgc cgctcatggc ctgagacacg gcgcccaagg ccttctccgt ttccatcgcg
      301 aagtaggccc gccccccctc caggctgtac tccagcactt ccatgccgga agcaaaccag
      361 gactggaact tctcctgcgc tccctcaccg gatccgatca aggcgatcaa ttcagggcag
      421 cgtcgaagat aggccagcgc cgtctcagcg tcacgctcgg cgaccagcag cccgtaacgg
      481 agcacctgag tccgccattc ctccgaaaga agacggcgga gcatcgcagg cgcctccgcc
      541 aataccgcaa tacccgatgc cggcgaacgc tcggccatct gcgctccgat gccgatcacc
      601 tgattgcggt gtgacggccc ggggatatct tccaatatgg ccggactcgt tctgaagaat
      661 tccaagactc ccaggtagtc cggcttgccg agactattct cgacgatgag cttcatgccg
      721 aaccgcaccc aggcacgaac ggcgtcgagt gacaacacgc gtgcaatcga gggaatctgc
      781 cggatatatt ccaccgccag ggcaaaatcg gtttccgtga gttcacaggc caggtccatc
      841 cacacgggcc aatccgccgg agataagaca ccgaccgctt ccggagcaat ccggataaat
      901 tccaccgcca cattggcctg acggtctgcg agttctaacc cggcagccag cacaatcgct
      961 ctgctggagg gctcgaggag accgagcagc agcggactct ccttaaaaaa tctcagggcc
     1021 aacgcaccgg aatctgccgc gatggccacc ccgagatcga cccatggcag cacatccgcc
     1081 aacaggccgc gacgctgcat atcggccaac gcatcgatcg cagccttggc cgctttcggc
     1141 gcctcttccg tgagctcgtc caacaacacc aggacaccat caagggcatc cggcctggca
     1201 gccgagtcgg caatagcctt caccacggca gcagccgcgt caggcccgag ttgttcggcc
     1261 agcaggtcac acagttgttc agaggagcgc ccagacgcca tagtgccaga atccgttttg
     1321 ggggaagttg tggattgtaa aggacacaat ttcgcttgta aactgtgtct tcgcggcggg
     1381 cagggaccga tggcgcacag gcaacgattc accgggcagt ggtaacgcgg gggacaggat
     1441 ggctgaaccg agcaaagaaa gcctcgacaa gatgtggaag tatgtaaagg gcttcgccga
     1501 gaagagcggg accgccatgc acccgaatga agccgtcacc aacgcagtgg tcctcggtct
     1561 cgccgcccac gtcgacgagt tggggaagcc gttatgcccc tgcaacttct accccgacaa
     1621 gcaggccgaa gccaagctgc ggcgctggat gtgcgcctgt gacgaaatgc agatgtacaa
     1681 atactgccac tgcctgctct tcgtccgtga agacggcatg ccgatcaccg aatatctgcc
     1741 ggaagaccac gaaggccgcc agtgttacgg gctcatcacc gatccgactc cggagaaagg
     1801 ccgcgcgctc cggcacaagg cactgccgat ggcgccgaaa gaaaccactc caccgaccac
     1861 cccggaatcc gccccgcaac cgtgatcgag tctatgcctc ccggccggaa tcctgccgtt
     1921 gccctcatgc tctccgccct gtctctcctg ctcggcctgg ctactaccgg atgcctccgt
     1981 acgatggagg cgatcgaccc gaggagcttg ccgattacct cgacctccgg ccaacgcttg
     2041 cagcaccacg tgtcgtttct cgcaagcccc gagctgtcag gacggcagcc gggctcaccc
     2101 gggaatcgcc aggctgcccg ctatatcgag gaccagtttc gagccgtcgg actcgaaccc
     2161 ctgccgtcac tcggcggcta ccggcaagcc atctcgccca cgatcggcga caatctcctc
     2221 ggcttcatcc cgccgaccgc cggatccgcc cccgcgcaat ggatggtgat cggcgcccac
     2281 tacgatcatc ttggttatcc attcctgggt gcggacgata atgcttcctc ggtcgccatc
     2341 ctcattgaaa ccgcacgaca catggcagga ctcccccagc acggcatcct gtttgtggcc
     2401 ttcaacagcg aggaatcccc gtatttcggt aaggctgaga tggggtcgca gttcttgttt
     2461 caccacctgc cgccggaaat cggccgtgcc gagaatctcc aggccgtcgt gatcatggac
     2521 ctcatgggtg gcgtacagtg gccccctatc aaggatgcga tttttgccgc cggcgcggaa
     2581 aaaagtccgg cgttgtacca gcgcgtcaaa caagcctcgg caccatcgct gacggtcttc
     2641 cctgtgggga tgcacctcgt cgaagagatt cccgaaatcg gccacaaagc cttcagcgac
     2701 tacgacgtct tccgcaacca tggactgccg tttctgtttc tctccgccgg acgcaccccg
     2761 cggtaccaca cggccggaga cgtcaccagc accctgcatt acgaccgcat ggccgccacc
     2821 gtcgaatggc tgcgacactt gattgccctg atcggtcagg atgaggcgcc ctacggattt
     2881 caggctgatc gtgtggagtg ggccgatgaa gtggtgtcgt tccgagagat cgtcaaccgg
     2941 gcggttcaag acgagacgcg gattccaggc acgtcgcgat ggtcgttgcg taaattgagg
     3001 caggatgcgg agtggctgcg ggatgccgat cgttcagcgc cgacgccgca ggaccgtgat
     3061 cgactggagc ggatctcgat ccgtgtgcaa tgcctgatgg ccgactactc cggctgtttc
     3121 accttttgat cgatccggcc tgtgcacgcg cagaacatca gatgcatcgt tattgccagc
     3181 cggtcgtctc atatcctttt tctttgagac aacgttcaac gaaggccgca tagtccggct
     3241 gcggttgcag tggtttgtag gtcccggcaa ggaaacccaa cacccccccg accgcgccac
     3301 cagccgccgc cccgatggcg atacccgtcg gcccgccgat caaccctgcg gacgctccca
     3361 ccgccgcccc gccgacaatc cccaacgtgg cgccggcgcc ggcgtttccg ctccgattgg
     3421 tcccgtgctg caaaccggcg cgttccgctt tctctccgca caccgccgcg tcacgtgccg
     3481 cctggtcttt gccatacagc tgtactcgag tgttggattt caggatgggc tctggcccgg
     3541 cacaggccac gagaccgatc gtgaccagga aaatcaggca gaatgacgat cgcgacgcct
     3601 tcacatccgc acctctctgc gtgccgaaca accggtttgt acccgcccaa ttgaacctgt
     3661 ctgccctgtc ctagtcatcg gattacctcg gatgcttttc cccgggcgac gcctccggac
     3721 agcaaggctc gcgatctacc cgccgctcat gtcattccag gtggagttgt gcgcgcacct
     3781 gttctaccga tcgccccttg accaagacgc gtttctggcg acttcccgcg ccgctcagga
     3841 tgtgaaccgc gcttaacggc acatcgcaga gacgggcgag aaatcggcac aattcgtcgt
     3901 tcgccgcgcc gtcagccggc ggcgcggcga tgcgaatctt caccgcgtgc ccatgcagac
     3961 ccgcacattc actgcgggag gctttcggct gaacatggac tgaaatggtc acgccctcgg
     4021 atgtggcgcg aacggcctgt tcgcgttcag tggcctgacg ctctcgcatg ggggtgcggc
     4081 tggtcgccga gctcctgcaa ccaccgcctc accgcgtcgc agtgggcgga tgcctcagaa
     4141 tatcgccttc acgacttcga tgatactgcg caacatgtcc ggatcatcgg taataggacg
     4201 ggcaatggcg acatcgcagg cttgatcggc cggtacgccc tgcctgatca atgtggccgc
     4261 ataaatcagc agtcgggtgc tcacgccctc ttccagtccg tgattcttga ggttgcgaac
     4321 tttctccgca atcttgacga gacgctgcgc aacctcgagg ctcacgccgg cttcatgcgc
     4381 caccacccgg ctctcgacat cgggcaaggg gtagtcgaac tcgatcgcca tgaaccgttg
     4441 cttggtgctc tgtttgagat ctttcagaat actttggtag ccggggttat aggaaatcac
     4501 gagcatgaag tcatcgaccg cttcaatgac ctgccccttc ttctcgatcg gcagcaggcg
     4561 ccggtcgtca ctcaagggat ggataatgac agtggtgtct ttcctggctt cgacgatctc
     4621 gtccaggtag acgatggccc cctgcttgac gccgagggtc agcgggccgt ccatccagac
     4681 cgtttcctgc cccttcaaca gataccggcc gacgagatcg gaagccgtga gatcttcatg
     4741 gcaggccacg gtaatgagcg ggcgccccag gcgataggcc atgtgctgga cgaaccgcgt
     4801 cttcccgcat cccgtcggcc ccttcagcat caccggcatg cgactctggg ctgcaatcgt
     4861 gaacaacccg acctcgcccc gcacttcggc ataaaacggc tcgcgcgcga tccgataccg
     4921 atcgatctcc tgttcctgcc ctttcggcac cgatgccgtt cgcccttcgc tcattccgac
     4981 actcctacat tgaattgaat aacctgcatg gtgcgccacg ataaacggaa acgagcggga
     5041 agttcaagca gccgactgaa gacagggaaa gccccgcgct cttgcgcggt acggtcaggc
     5101 gactttcgat ccatcgagca cttctcgaac cttttgcgcc agggtcgtgg gtgtgaaggg
     5161 cttctgcagg aaggaatgct cggtatcgat ccctcccttg gaaaaggccg ggtgatccgg
     5221 ataaccggac atgtacagca ctttcacctc cggacgaacc gtcgcgagtt tttcggcgac
     5281 ttccggtccg ctcatctgcg gcatgaccac atccgtgagc agcaaatgaa tcggaccggc
     5341 atgtttcgcg ccggtgagca acgcttcaat cccgtgccgg gcctcgagca ccgtatatcc
     5401 atgcaagcgc agcgtttcat tgaccaaccg tcggacaccc ggctcgtcct ccaccaacaa
     5461 caccgtctcg cgccctctga ccgagtcccc tccgaccgcg acggattcgg tctccgttgt
     5521 ggccgattcc acacgcggga agtaaatctt aaaggtcgcc ccgcgtcccg gcttgctctc
     5581 gacctcgata cagccgccac tttgcttgac gatgccatag accgtcgaaa gaccgagccc
     5641 cgtgcccttg cccttctctt tggtcgtaaa aaacggctcg aagacgtgcg actgcgtatc
     5701 ctcgtccatg ccatggccgg tatcgcgcac gatcaacgcc acataggaac cgggctcggc
     5761 gcccatcggc ggacggttcc tgcgttgccc gaactgcaca ttgctggttt ccaccgtgag
     5821 gcggccaccg ttcggcatgg catcgcgggc attcaccgca agattcatga tgacctgctc
     5881 cacctgaccg ggatccgcct tcagctgccc caaccccgga tccagcaccg tgcagagctc
     5941 gacgatgtcc tcgccgagca accgtcgcaa catgccttcc atattgtgga tcaccgaatt
     6001 cagatccagc actttcggcg caatgaattg gcggcgactg aacgccaaca gctgtccggt
     6061 gagaccggag gcgcgatccg ccgccttctt cacttcctcc agttccttcc ggaatccatc
     6121 cgtgggcgcc aggcggctca acaccagttc gctgtatcca cgaatcaccg tcagcatgtt
     6181 gttgaagtcg tgcgccacgc cgccggccag ccgtccgacg gcttccattt tttgcgactg
     6241 ctggagctgc gcttccgtgc gccgcaacgc atcctcggcc cgcgcgcgtt caccgaactg
     6301 gccgattttc agtccgacat cggccaccat cttcaatagc tgctcgtccg gctgcttgat
     6361 ctcgcgccgg aacaactcga tcaccccctc cacttcggtg ccgacccgaa cgggaaatcc
     6421 gaaggccccg tgtaacccca ctctggcagc ggcaccaccg cgagggaaat tggcctcctg
     6481 caccacgtct ttgatccacg tcggttcccc ggcctgccaa atccgtccgg ggagtccctt
     6541 gccgatcgta aaggtatgtc gccaggtgtc caacacaaac tcttcggcgt tcaatgcggg
     6601 cgactgccag acatccagac aacgcagcac acttccctgc cgatcgagcc gccagaatat
     6661 tcccaactcc cattcgagac tttcgcagat ggcctgcagg atcttcggcc ccgcttcttc
     6721 cagcgtccgc gattccgcca agacgcgcgt gaccgcgtac tgagacgcga gttggcgctc
     6781 ggtacgtttc cgagccgtaa tatcccggat gaacgcgctg aaaatatagg catcgcccag
     6841 ccgggcagga gaaatggtga tttcgacggg aaactcatgg ccgtcgcggt gggatcccat
     6901 catctcaatc cgtcgattga ggagtgcgcc ctgcccggta tccaggaaat ggcgcagtcc
     6961 gcgctcatgc tcgtcccgat cgcgcacagg aatcaccgtc tcggacacgc ggcggccgag
     7021 cgcttcctcc cgctcccagc cgaaaatatt gaccgcctgc gcattccaat ccgtaatgat
     7081 gccgcccgca tccatagaaa tgacggcgtc cagcgccgta tcgacgatga cccggttgcg
     7141 ttcctgactc tgcagcaacg cggcctccgc cgcacgcgcc cattcacttt cctgctgcgc
     7201 ctgccggtgc tgttcacgca atcgaccggt ggcccagagc aacaggccga ccatcgggag
     7261 ccagacgacg acaccggcca tgcccagttt ccacatcatg gctcgaatcg gcaggagaat
     7321 gaggtcacga tccatccgca gcaggatcgt ccactgcaat ccggaatagt cgctataccc
     7381 cttggtttga gaataacccg tgacgaccgg gacgtgacgg cgaacatgta tctcttcgac
     7441 gtagcccgcc ttgccggacc ggctcaacag caccgatggc aacccgagtt ctgtgagatt
     7501 cacccgatcg ataccggagg aagctgagtc cacaaacacg acaccggctt tcgtcaggaa
     7561 ttgatactcg aaggggccgg cggagccatc ggtttgttga atttcaccga ccgtttcaat
     7621 gaggacatcc tccagcatcg gcagcccgat gcgggtcgtc acggcaccga gaaactggcc
     7681 gccgacactc agaatggggg ccgtaaaggc cacggactcg atcttatgat tcgattcatg
     7741 gatctcgact tcatccacct gcacggttcc ggtctgccgc acccggtcga accagccgct
     7801 tcgactatag tcctggccga tcacggagga gtccgtcgcc gccaccatac gcccatgggc
     7861 gtccgtcacg cccagccaga gatagaccgg cgcataggtt tccttcatcc actgcagata
     7921 ttcgttgatg tattttttat cgagggcgcg atcggaaaag gctcgagcca tcatgcgaac
     7981 atccgcatga cgttcgaaca acaaccgatc gagtttgtcc gcgatttcag cggaggccag
     8041 cgtcagattt tctccggtcg cctcgaccat tcgcacttca atggagcgga gcatcaccac
     8101 gccgatcaca agcgccacaa tggtcacgcc gacgatcagg accggcaacc agggatagga
     8161 ccgacgcggg gaaccggctg gaagcgaggc ggccatactc aggactccgt cacaatgtag
     8221 tttggtaaca atcgtgcggg atgataccac tgcttggaac gggccagact cggcaggccg
     8281 gaatcatcca tcgtgttgat ccaacatgcc cctttcgcgc gagcttggcg acagaattca
     8341 cgaaacacaa acggcccgag ccccacaata tcccgatcgg cgacttccag caacacacaa
     8401 aacaccgacg ggttgagcca caggcccatc gtataggccc gaatacggcc tccaacccga
     8461 accactgcgc cgatcagccc gagttcggca gcgtcagaca atgccgtctc atgcgcgccg
     8521 gcggcatcct ccaacagcgc acaggcccag tcatcagaac cggcctgctg cttctgttcc
     8581 cgccattcat gaaacaagct gacacaggct ctttcgtccc gcggctcata gggctcaagc
     8641 atcccgcccc gttccctgat aaatcgattg cacgcggccc gcggggattt aaacgactcg
     8701 ccggacaagt cggacagagc tgacgcgtcg tagagatagt cgggctcttt cgcagtcgca
     8761 cggtagccca gtgcgcgaac ctcatcgaca caggcctccg gcacattctc gacccgcgtg
     8821 acccgggaat cgccgttccg ctcacgcatg aaacgcaagg cctcggccaa cggctgcgcg
     8881 agaggaccag gcccaagcgg gggcagggcc atgaacaggc catcgggaca gtcggcgaac
     8941 aaacagagat ggtcgtggag aacagcccag gaataggtca cgcgatgtcg ccagatgtaa
     9001 tggtaggcga aggaagcggc tgcgagcggc gttccctgaa caccggcgct gacggcgagc
     9061 gccgcgtcca tgcgcccccg atccccgagc gtgaaggcct gcgtcctcac ccgcattcgc
     9121 ccctgctgca gccgttcggt cacgcgttcg agcggccgca acacgatcac gtcctcctgg
     9181 aatcgcccaa tgagcctggg ataccgcaca agcgtctcca ccctggcatc ttgctcgatc
     9241 catccggcaa tttcgtcggc agcctgagcc acgagcggtg aagactggtc cctcatatac
     9301 ggacacttcg tgtcccatcc caacagcacc tgctcatgcc tggcatccca catcaaggca
     9361 aacgggtaga gtcggcaatc gaggggacgg acctcataga tgcgacaccg cgaggttgcg
     9421 caatcgaagg ccggacagac gtacccgtcc ccttccggat tcggcaccag atcgatttgt
     9481 gagccatcgg ggtttgggaa caacgcaggg gcaagcccgg ccgcgattgc ggtctcgatt
     9541 tcgtcagcgg taaagaaggg ccgcaggaaa ctgtcccgct ccgggaaccg gcaacagaca
     9601 tcgcaactga ggcaggcgga actcggcacg atctgcagga gagcatcacc cccgggacga
     9661 gaaggatttg tcgggacaat tggcacgaat gaactcgtca tgagctgtgg gcgcattgta
     9721 gcactaacaa gtggagcgtc ctactattcg aaacgtttca cccccgcgtt gccgcgctct
     9781 cacggggcgg taacgagtcg ttcttcgaac agctccggcg ctccgccgac ttgtaccggt
     9841 gtcagaggcc atggtacgat ttgctcacct cgaatccaaa cgcgagtgcg aggcatgcga
     9901 ctgcgctgcg agtctcgagg atattgcatt ggcactggca caacattcac gtgagccatt
     9961 gaactccgac gaagcccgcc gacgttgtct cgcaattcgt gatgttctcg ttcgcgccgg
    10021 ggtctacggc ccggccgaaa ccggcgacgg cgcggcaacg accgactcct caattccgac
    10081 gccacggttc cgcctggcgc caacgccatt tccgctctcc tcctcggacg tcacgttttt
    10141 tcaggaactg ggacaggacc tcctctcctt ctatcgggct ctgaaccgtc tgtatcagga
    10201 cagcacccgc ggaactcagc ccgcctgggt ggcggcctat ctcgaccagg gcaagccgga
    10261 atcgctgatc acctatagcc ggatgaaccg tttccgcacc ctcattcccg gcatcatccg
    10321 acccgacgtg attccaaccg ccgatggtat ggtgatcaca gagctcgatt cggtaccggg
    10381 ggggatcggc ctgaccggtt gcctgactcg cgcctatgat gatcagttca ccatcgagcg
    10441 ccagcccccc ggtctgccgc ctgagcgttc tcctctgatc gcggggcgtg acgggatgtt
    10501 acaaggattc gccgccatgc tgcgcgccca gcaaggcgac caggaagggt gcctcgcgat
    10561 tgtcgtttcc gatgaggcca aggaataccg ggcagagatg gaatggatgg cccaacgatt
    10621 gcgtgagatc gggtaccagg ccttctgtgt cgaaccacgg gcggttcatt tctcggaaga
    10681 gggcctgttt cttcaggaag aaaccggacc acggccgatc ggtttgctgt accgcttttt
    10741 cgaactgttc gacttgaaga acatccccaa ggctgaactc gtgatgtatg ccgccaagaa
    10801 ggggcaggtg acggtcacgc caccctacaa gcccgcgctt gaagaaaagc tggcgttcgg
    10861 cctgtttcac catccggaac tggctccgtt ttgggaacag gcattacgtc ccgaaaccgc
    10921 catcaacttg cgacgattgc tgccgagaac ctggatcctc gacccacaac cggtgcctcc
    10981 ttccgcggtc atcccgcacc tcacgatcag cggtcgcgcg gtgtcggact tcaggcgctt
    11041 agcccagacc acccaaaagg aacgccagtt agtgctcaaa ccatcaggat tctctgagct
    11101 ggcctggggc agccgtggcg tgtccatcgg gcacgacctg ccgcaggtcg agtgggccac
    11161 ggcattggag caggcgctcc aggcattccc gacgacgccc tatgtccttc aggaattcca
    11221 caagggccgc ctgttcgagt tgtcctattt cgacgagcgg agcgacacgg tggtcccgat
    11281 gtccgggaga gtccgcctgt caccctatta tttcgtcgtc ggcgataagg ccgaactcgg
    11341 cgggattctc gcgaccctct gccctgccaa caagaaaatc atccacggta tggtcgacgc
    11401 gatcatggcg ccctgtgcca tcgcgccaaa cctcgcgtca taaggtgacc tgatgatcgc
    11461 tgcagcagaa ccggttcatt tgcagatatc tactcaacct ttttccccat aggtactcat
    11521 ggccctgacg ctctatcatg tccaatggtg cccggattgt gcggtcgttc gggaccgtct
    11581 ggacgaactg aaactctctt acgaagacgt ggtggttccg gactttcgtc cgatgcgcac
    11641 acaagtcttc gaggtgtcgg gccaatacta tgtcccggtc ctgaaagacg gagacaccgt
    11701 tctgacggaa acccacgaca ttcttgctca cctgcacaca cagtacgaca aggcacgccc
    11761 gtgaggatct cgcccggatc tccagcgcga gcgccgatca cggaccgacc ccgatccctc
    11821 gggcaggcgc cggcctgcac ggctcggcca cttagcaaat ttttgacaaa cagtcgtcgc
    11881 ccatgtgctt ttcagagccg gctgcgatca cgtatttcca tacgcatcac ctacaggccg
    11941 ttccaaaagg ccgcccagca aggccgcagc gagcgaaacg gcgaatcgta ctcgtgccgt
    12001 acggtgagcc gctgagcgat gcgagaacgc cgctggcgga ctttttcaac agcctgttag
    12061 aaaggataga gacgtggagg actggagacg catcctggct cagagcgtgg tcaagccgaa
    12121 ggatttggcg gatcggctcg gtgtcgatcc gaaggaaatc gaggacatcg tgggggacta
    12181 ccccatgcgg attacgccga ccgtgctggc caccatcaag gaaaagggcg atgcgatctg
    12241 gaaacaagtc gtgcccgatc gcgcggagat ggccgatgcg gatgcggaag acgatccgct
    12301 cgaagaagat ctgatgagcc ccgtgcccca cctggttcac cggtaccccg accgcgtcct
    12361 cttgatggtc acaaatcagt gtccgattta ttgccggttt tgcacgcgca aacggctggt
    12421 gggcaaaccg ggcttcctca agaaagggga gctggaccgg gcgatcgcct atctgcgcga
    12481 ccatcaggaa gtgcgggatg tgattttgtc agggggcgac ccgctcctgc tgcccgatca
    12541 cctactggaa cggattctca aatccctccg gaccattccc cacttggaac tgatccggat
    12601 cggcacacgc gtaccgggca gcctgccgga gcgcatcaca ccgaagctct gcgagatcat
    12661 caagaaatat catccattct atatgaacct gcacttcaac cacccggacg aactgacccc
    12721 ggaggtgaaa cgcgcctgcg ggatgctggc cgatgccggg gttcccctcg gtgctcagac
    12781 tgtgttactg aagggggtca acgacgaccc cgagatcatg aaacgtctga tgcatcaatt
    12841 gctactggca cgcgtgaagc cctattacct gtatcaagcc gacctgacga aggggacgaa
    12901 tcatttccgg acgtcggtgg aaaccggcct caagatcatc aagtccctgc agggccacac
    12961 cagcggcatg ggcgtccccc actttgtaat cgatgctccc ggcggcggag gcaaaattcc
    13021 actgttgccc gcggactacc tggtcaacct ggacgaagac agcgccgtgt tgagaaatta
    13081 cgaaaacagg accttccact acccccagcc tggttccgct ccaggacgcg aactgccgat
    13141 ggtcggcgcc catccttcct gggcctccac aggaaacggc tgcgacggag ggggtgaaca
    13201 cctgtgaccc gtgccgcttc ttcaaaaggg attctgccgt atcagcacat cacgcagctg
    13261 attgcccgtg agtccatcac atcggaccat ccgatcgaag accggcaaat ccagccggcc
    13321 agtctggact tgcggctcgg aaagaaagcg taccggctga tcagcagctt cctgccggaa
    13381 ttgtcggcta tcagcagccg cctcaatgcg ctcgactttt atcagtccga cctcgtgatg
    13441 tatgagatgg acctcacgca gggtgccatc ctcgagaagg gacatgtgta tctggttccg
    13501 ctcctcgaac aactggacct gccggccacg ctccgggcac gcgccaatcc caaaagcacc
    13561 accggacggc tggatgtctt cacccgcgtc gtcaccgacc tgaatgccgg cttcgatgaa
    13621 atccgggccg gctaccgggg cccgctctat ctggaagtgg tgccacgctc cttcgccatc
    13681 aaggtgcgca cgggtgactc cctgaatcag attcgtttcg tgcgcggcga tgccacggtc
    13741 tccgacagcg ccctgcagaa gctgcatggg accgatccgc tgctctttcg taatgcctca
    13801 cccccgaagc cgctgccgca gaaggagttc cgtacggagc gcggcctctt cttgcggatc
    13861 gacctcaccg gaagtgcgcg ggaagatgcg gtcatcggct accgggccaa gaagaacagc
    13921 catgtcatcg atctggccaa aatcggacat tatgcggccc tggacttctg ggaaccgctg
    13981 aaacggcacc ggcaagacag cctcctcctg gaaccggagg agttttatat cctggcgtcg
    14041 aaagaacgca ttcgggtccc gcctgggtat gccgccgaaa tggtcgccta cgaagcggcc
    14101 tgcggcgaac tgcgcaccca ctatgccggc ttcttcgacc cgggcttcgg gtacggagac
    14161 ggcaagatgc ggggcactca ggtcgtcctg gaagtccgcc cgcacgatgt gccgttcctc
    14221 attcatgacg ggcaaacctt cttcaaagta gtgtatgatc gaatgctcac ggtgccgaca
    14281 caggtatatg gaacggcact cggctcgtca tatcaacgac aagccctcac cctcagcaag
    14341 cattttaagg cctagacatg gcaccaccca gcgaccttcc agatccttcg acccgtccgt
    14401 ccaggccccc gttgcaccgc ccggacgacg aaagcaatgt agaccgccag acgcatgtcg
    14461 tcgggatgat ggtcggcacg gccttgatgt tcatcggctt tttggacgtg ttcctctcta
    14521 tcagcggtgg attcgagatc aacgtcgtgc ccctgctgat ctacttcagc ggcattgcgg
    14581 tctgggccaa tgccgtggtc gaaaatccga cgatccggta ctccttgatg gcgggatcgg
    14641 ttctgatcag cctggcattt tttcactatg gcgaagtgct gttctggcat aagcaggtgg
    14701 tgttctggtc caccgtcgtc gtggtcatgt atttcatgtt caacgagccg aaacaggcaa
    14761 cctgaagcac tccgtgccct gcccatcttg agatcgggcc ggtaaattca tcgctcctct
    14821 tcccgcgccg atgaccattg ccgtcatcat cccggttctc aacgaagcct gcggtatcgg
    14881 gcagaccctc tcacacgcgg ccacgctcgg attcgacgag ctcatcatcg tggacggcgg
    14941 cagttcggat cagacctgcg ccgttgtgga gtcgtgtgcc ggccgccttc agaacaggcc
    15001 ttgctcaggc cccgcaccag cctcgccggc tatccgtctt ctgaaggcgg ccgcggggcg
    15061 cgcgcgccag ctcaacgccg gtgcagccgg cagccgctgc gaggtgctcc tgttccttca
    15121 cgccgatacg tacctcccca ccaacgcccg gcaggccatc tccaccgcgc tgtccgacga
    15181 ggcttgcgtc ggcggacgct tcgacgtccg cttcgactcc cctcgtccga tagcccgact
    15241 cgtcggacac atgatgaatc tgcgctcccg ctggagcggc attgccacag gtgaccaggc
    15301 cctcttcgtc cgacgggatg tcttcgagcg gaccggacgg tttgcggaga ttccgctgat
    15361 ggaagacatc gagttctccc gtcgcctcaa acgagcagga cgccttgccc cattgcgtag
    15421 tcaggtcgtc accgccttcc gtcgatggga acggaacggc cccgtccgca caattctgtt
    15481 gatgtggacc ctccgcttcc tgtactggat cggagtcagc ccctatcgac tccaacattt
    15541 ttatagcatt gtgagataaa cccatgagtg ctcccccgtc ggaccgcaaa cagcccgcat
    15601 cggccgccct ggtgatcttc gccaaagccc cgatcccggg acaggtcaaa acacgtctct
    15661 gcccgggcct gaccgaagac gaagccgcca cactgcacgg cagcttcgtc ctcgacaccc
    15721 tcgaacgcac gaaggcggcg gtacataagt tcaagcttcc tgtcgatcgc tttctggcct
    15781 gtgcgccctc aagcacccat gtctttttca gaatcatgga agcgcgccac ggcgtcacgc
    15841 tcctcgatca accaggcgac aacctggggg acaggatggc gcaggcgttc agcacgctct
    15901 ttgcccgtgg gtaccggcac gttttcctgg tgggaaccga cgtaccctct ctgcccctga
    15961 cacattatcg cgatgccgtg cagtcactca cccggcacga tctgatcctc ggtccggcaa
    16021 aagacggcgg ctactatctg atcggcatga cggcgccaca cccagagcta ttcgcggata
    16081 ttccgtggtc gacggaccag gtcatgggac tcacgcagca gaaagctgcg gccgcgggat
    16141 tgaagaccgc cctcctgccg gcctggagcg acgtggacac cctcgacgat cttcacgcct
    16201 tgatcgatga ttgcgcagcg gacaaaaaac gccccaaaca ggaacgagtg tattcgcaac
    16261 gaacggccgg tacgctggag ttgctcgcga aacgcctgcg cacgcgtgag gcctaatagt
    16321 ttgtcgacaa actaaataac gaccccgaaa atggagttgt gcccctcatg cacagacaga
    16381 accggaatac cccgcaggct gttcagaaag gccgtccagc aaggccgcag caagcgaaga
    16441 ggcgaatcgt actcgtgccg tacggtgagc ctcggcgcga tgcgagaacg ccgctggcgg
    16501 actttctcaa cagcc
//
LOCUS       DAOQRM010000047        16492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2599_length_16492_cov_16.759871, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000047 DAOQRM010000000
VERSION     DAOQRM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2599_length_16492_cov_16.759871"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PKK81_05745"
     CDS             complement(<1..320)
                     /locus_tag="PKK81_05745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV32407.1"
                     /translation="MPQHPDEGRVELTDWTTTESAIRAIRETVFIHEQGVPVELEWDG
                     LDSSCAHVLAWNDRGEAIGTARMQRNGTIGRMAVLKYWRGRGVGRDLLQTLLDLAVRQ
                     GLPRV"
     gene            503..766
                     /locus_tag="PKK81_05750"
     CDS             503..766
                     /locus_tag="PKK81_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32408.1"
                     /translation="MKTQRRTYDVVAGRRIRSGLWHSWCQAGGRAEATRVFGWSFLRS
                     VWSLLRKVTWGRIGREQESRSPWGHKSIGCSAPRSTAIYGRMN"
     gene            1038..1892
                     /locus_tag="PKK81_05755"
     CDS             1038..1892
                     /locus_tag="PKK81_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV32409.1"
                     /translation="MKVLIATDGSKYGKWATEWVARMPFAEKPDVSVLHVTDVEALRA
                     PFMFQPVVIGNEPFIQEEIKRIEARGKTAMAEAKAQMASLKLKGKLVSERGAAGSTIL
                     DRAPKRDGLVAIGSRGLDALDRFMLGSVSTQVTLHAPCSVLIVKEEPRPLSRILFAAD
                     GSKASEKALRFLLTKLHPEEREGLEPIDVVVMHAMPFLKYPELKEAGARLVEQCANKL
                     LKAGYVVDEVVQLGKPADEILKVATKKKVDLIVTGAKGMGAVARFLLGSVSTKVVQHS
                     TCSVLVVR"
     gene            2485..3582
                     /locus_tag="PKK81_05760"
     CDS             2485..3582
                     /locus_tag="PKK81_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV32410.1"
                     /translation="MAPSAGKPVNRAANGMVQLTDPEIARAGIEVMTVKKEPFTFHRE
                     FPATVQANENELAEVTTLIRGRVVEVLVDVGKDVKKGERLALLDSTDLGMAEGAYLKS
                     AARQHEAQLAFERAENLHEHRAISLAELQRREAEMKTARADAREASHRLALLGLPAQE
                     IKRLEREQTIRSDVAIRAPFAGRVIMRNITRGEVVETSRNCFTIADLSDVWVVANVPE
                     KDVRFIRPKDRVHVMVPAYPHGLFSGTVTYISDVLDPSTRTMRVRVTVLNPDRTLKPE
                     MFAMVRVFASPQPEALAVPLTAVQQDGTGKLLFVRQADNRFESRRVLLGDELDGKTIV
                     LEGLHEGEDVVVKGSFAIKSEFDIHKIEPTP"
     gene            3579..>5468
                     /locus_tag="PKK81_05765"
     CDS             3579..>5468
                     /locus_tag="PKK81_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV32411.1"
                     /translation="MIASLLEFSLRQSILVIGLACMFSVAGLFAFQSIPIDAYPDVTN
                     VQVQVLTEAPGLSPVEVERFITYPIELQMNGLPGLTEIRSLSKFALSQLTVVFEDDVD
                     VYFARQLVLERIMAVKERLPAGIDPVLAPVTTGLGEIYQYYLEGPQAHATDTALVEAE
                     LTNQRTVQDWVLRPLLKSVPGVIDVNGLGGFVKQFQVLVDPDKLRKYSLTLHDIYEAV
                     QKNNANSGGNVLERHAERAIVRGLGLIKTLPDIERIVVKEAGGTPVFVRDVAEVRIGH
                     AVRHGAAVLNGEREVVAGTVLMLRGGNAREVVQAVKRRVDTIQQDGLLSEGLKIIPFY
                     DRIELVTAAINTVRDALIEGIVLVTIVFFLFLGHVRSAIVVTVSLLVTPLMTFLVMQR
                     VGLSANLMTLGGLAIGIGEIADGSLVVVENVYRHLSDNHLHQRSRLEVIFRATSEVGR
                     PIVFGILIISVVFLPLMTLHGMEGKMFAPLAYTLVISLLMSVVVTLTLSPVLASLILR
                     GDHPEETRLTRWMKARYQPVLRWTLDHRVPVLLGSTAVVLASLTLLPFVGREFIPILE
                     EGALTPQIVRLPSVSLPESIAIEKQTQKVMLEFPEVQMSVSKIGRPDIAVGPEEPNES
                     DPIVTL"
     assembly_gap    5469..5568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5569..6367
                     /locus_tag="PKK81_05770"
     CDS             <5569..6367
                     /locus_tag="PKK81_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV32412.1"
                     /translation="RNSIGAIGDIRVRSASGAPIPLSELATIEMREGPARISREQAKR
                     RIYIGFNVVGRDIGSVVDEGRKLLAERIRLPQGYTVAWGGAFENMERANARLLLVVPI
                     TLGLVFFLLFWAFHSLRYASLIILNLPFALIGGVVSLWLSDQYLSVPASIGFIELFGL
                     AVGNGIVLVSYINQLRNEGQQTEAAIMTGCVLRLRPVVMTMMTTLLGLLPLVLAQGIG
                     AEVQRPLATVVVGGLFTSTALTLLVLPALYRTFAEQEAAKEHAPEWV"
     gene            6385..>8831
                     /gene="mgtA"
                     /locus_tag="PKK81_05775"
     CDS             6385..>8831
                     /gene="mgtA"
                     /locus_tag="PKK81_05775"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNV32413.1"
                     /translation="MENRRPFWACSPDEVLRDLRAVPEGLSTVEAEQRQVVSASARLT
                     PQRDSQPFRLLLAQFRSPIILILLFASGVSFFLAEHSDALIILAIILVSALLSFWQEY
                     SAARAVAGLLALVQITAQTWRDGRLREVPADDIVPGDIVELSAGSSLPGDARLLDAKD
                     LFVDEATLTGETYPAEKSTGTLTDAVPSQKRTNSLFLGTHVVSGQARAVIVAVGKDTE
                     FGRIAHRMQVRAPETEFERGVRRFGYLLLEVTLLLVFSIFAVNVYLARPVLESFLFSM
                     ALAVGLTPQLLPAIISVNLSHGAKRMAQQKVVVKRLTSIENFGSMNVLCSDKTGTLTM
                     GSMRLHAALDLQGRPSEKVLFLAQLNAMFETGFSNPLDEALRRHRSVDLTGYRKLEEE
                     PYDFVRKRLSILVATPQTHVLITKGAVDSMLTACLDAEQSDGTVVSIDEVRDSIRQQV
                     RELSAQGFRTLGLACRNMGAVDRVSKEHETAMTFLGLVAFADPPKPGMAETIATLKGL
                     GVSLKMVTGDQALVAAYVGRAVGLENPHLLTGTDLRGMSDDALRTRANSIDIFAEIEP
                     NQKERIIRALRASGNVVGYLGDGINDAPALHAADVGISVDSAVDVAKEAADLVLLEHD
                     LGVLVQGVREGRMTFANTLKYVFMATSANFGNMFSMAGASLFLPFLPLLPKQILLTNV
                     LTDVPEMTIATDRVDHELIERPRRWDIPFIRRFMLTFGLVSSLFDYLTFGVLLLLLHS
                     TAGQFRTGWFVESVLSASLIALVIRTRRPCYTSRPSSALLITTLLTGLGTMLLPVTPV
                     GAFLGFEPLPPIFWAAML"
     assembly_gap    8832..8931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8932..10426
                     /locus_tag="PKK81_05780"
     CDS             <8932..10426
                     /locus_tag="PKK81_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32414.1"
                     /translation="SNTPSYDPQVLLDSQPVTVNVIDPADHSVQFQNRTSLEIFSDMA
                     GSRCHAKIAGKAAPCEFCRMPEALARGGVVSEEVDMPDGRRLLIHWAKAPTTDGRVHV
                     IETIVDVTKRKQDEQSLRQSQKMEALGRLAAGIAHDFNNLLMVVIGHAQRVAQQLGSH
                     PCHHEVEMIGQAGSRAAALTKKLLTFSRRQVLEQRDVPLNTLIRDMEDILRRLIGEQI
                     QTVIVLDPRAGHVLGDPVQIEQVLLNLALNARDSMAEGGILTIETGNADLDEAYVRVH
                     PGAVPGPYVKLVVEDTGCGIDAETLAHIFEPFFSTKEFGKGTGLGLATVYGIVKESRG
                     YIDVTSQPGRGSRFTVMWPRVLTQTGEAEPVAASRAVPAACATILLVEDDEGIRRLVS
                     AVLRDQGYEVLAATDGVEALQMLQLRKGGCDLLITDVILPRMKAAVLAQGARTMFPNI
                     KVLYISGYSGDMLVSHGVDAQAAYLQKPFLPQAIIEKVAELLPARPQ"
     gene            10659..11675
                     /locus_tag="PKK81_05785"
     CDS             10659..11675
                     /locus_tag="PKK81_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNV32415.1"
                     /translation="MLASWTILVLSGAMIVMAGTKLSRCGDQIAALTGLGRLWVGVVL
                     MAAATSLPEVFTTVSAGLLDAPDLAAGDLFGAVLANMFTLGLIDLIHRDKRVWEQAAF
                     EHTLVAALAMLLTGLAAFLVLFRSGTGASAIEGGSALLFLAYVFGMRMVFRQEDVKRR
                     QRAQQLLAEAEADDDHTRESVRRDGLRRAAIGFVAATVVLIIAAPLLAKSAKDIADQS
                     GVSTTFVGTSLVAVVTTLPELVTSLAAVRLGAFDLAVGNLFGSNAFNMAAFLFTDLAY
                     AGGPLLGAIDSTHALTALWGILMMNVGLMGIVYRVEKRYWLIEPDSLVMILGYLLGLW
                     LLFR"
     gene            complement(11962..12330)
                     /locus_tag="PKK81_05790"
     CDS             complement(11962..12330)
                     /locus_tag="PKK81_05790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV32416.1"
                     /translation="MSKRKNTRVEMNRPIELQGPRGTSQGVVRDFSPGGCRIEQPDAK
                     VHCGMRLTLRISLPDRREPIEIKPAVVTWTGKDSFGVEFLSLAQDVRARVKIVYDLLL
                     DAQSSAESERVISLPSVSWT"
     gene            complement(12476..>13372)
                     /locus_tag="PKK81_05795"
     CDS             complement(12476..>13372)
                     /locus_tag="PKK81_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32417.1"
                     /translation="APIALVEQPWAVSLDMTGYRVQIDGVKPDGRRYVFATNSAAAMH
                     LSVTLEAVSGQATEQGCLAHLERIARTSPEQPQQDITRYEIRQVPVLEYLLPETKGTA
                     VDQLHLFACVRKDNVYADIHVAKTGFTTGDDSRLRAVLQSLDIVPAPLAGSLDHFRAG
                     SAPYLQGQFRQAIPHYEQAITLERAHSTLGKAVWRLLVHNLGVAYGMTGDLARAKTTL
                     DYGLSRDPANSLFHYHLARTYAGMNDREKAMQSLHAAFRNDRQRETGDRIPDPRQDVS
                     FRRFMLDPTFRDLAESLMQPAI"
     assembly_gap    13373..13472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13612..14211
                     /locus_tag="PKK81_05800"
     CDS             13612..14211
                     /locus_tag="PKK81_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32418.1"
                     /translation="MQQRTTGLLGFRRRWRVACLLAASLMLAGPSAHATVQVLPEMTL
                     KREGRTITGAKAIRADQTMQDILTAFDRAEHALERQDLSALMQFYASGYNYHGLHVAD
                     VERIWGEVFEHYRALSSTHLFSEMKVVQTEEGTRVELTCTGGLYGTEAQSGARITLDS
                     WFQEVHYLVRDKNGWRFLGNRGDAPRSAPFSSSPHHPLF"
     gene            complement(14222..14674)
                     /locus_tag="PKK81_05805"
     CDS             complement(14222..14674)
                     /locus_tag="PKK81_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32419.1"
                     /translation="MTRSSWSAPNPIPDSLRRSLKDLRHGLLGLHKSLIVSEQVTYER
                     IYGRVSSAGQLLQLVMNDPWFAWLHPLSHLVVRIDVVLEDQDDTTLEAAQDLLSEART
                     MLRPSEEGDGFERSYYEALQRTPDVVLAHASVKKLLGTAATAASAAAA"
     assembly_gap    14858..14957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14958..>16492
                     /locus_tag="PKK81_05810"
     CDS             <14958..>16492
                     /locus_tag="PKK81_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNV32420.1"
                     /translation="NELPEAPPPSPWTILGAQFTSLIVWVLIGAAIVSGLLGEWVDTG
                     AILAIVLLNGFLGFVQEYRAEQSLAALKTLAVTYARVIRGGSRRQLSSTELVPGDIID
                     VEAGDHIPADARLIQAAALRTQEAALTGESTPVDKSGGVLPDNDLPLADRRNMLFLGT
                     TVTGGKGRALIVATGRGTELGRIATLMTSVPVEPTPLQRRLEQFGHVLLLLSLGIVVV
                     VFVLGLWRGEPVFDMFLTAVSLAVAAIPEGLPAIVTTTLALGVMRMVTRHALIRRLPA
                     VETLGAATVICTDKTGTLTKNEMTVTRLALDGQVYEVTGEGYGPEGDIIGGDPREGGL
                     QQLLLSALLCNDATLKEVDGSWKVVGDPTEGALLVAGGKAGWRKEDLERAHPLVGEIP
                     FDSERKMMTVVRRSGADIVAYVKGAPDILLGRCRDFLSVEGQVQPLTDSMRESILSIN
                     RQFAEQALRVVALARRTLDQEPSTYDSETIERQLVFLGLAAMKDPLRPEAKVAVELCR
                     SAG"
BASE COUNT         2885 a         4532 c         5312 g         3363 t
ORIGIN      
        1 acgcgcggga gcccttgcct aaccgccaga tccagcagtg tctggagaag gtcccgtccg
       61 acgccgcgcc cgcgccagta cttgagcacc gccattctgc cgatggtgcc attccgttgc
      121 atgcgcgccg tgccgatggc ctctcctcgg tcattccatg ccagcacatg ggcgcaagat
      181 gaatcgagcc cgtcccactc cagttccacc ggcactccct gctcgtggat aaacaccgtt
      241 tcacgaatcg ccctgatggc cgactctgtg gtggtccagt cggtaagttc tacccgtcct
      301 tcgtcgggat gttgcggcat gattgtctga cctcctcaat ctgcgcggtc ttctgcatcc
      361 gaatccggag agacgcgcgt ctcaaatgta attctctttt ggaggatcgg caaccacatg
      421 gtaccgttca tcatgatggt ctgcctgcga aacgacgcag gcggacgccg agtggctccg
      481 gttcaccgat gggaggtcat ccgtgaaaac tcaacgtcga acctatgatg tcgtggcagg
      541 gcggaggatc cggtccggat tgtggcattc ctggtgtcag gccggtggaa gggctgaagc
      601 cacgcgcgtg ttcggctggt cgtttctgcg ctcggtgtgg tcgttgcttc gcaaggtgac
      661 ctggggccgg attggtcgtg agcaggagag tcgctccccc tggggacaca aatcgatcgg
      721 ttgctccgcg ccgcgatcca cggccatcta tggtcgcatg aactgaccgg cggtattccg
      781 ttcggttgat tgccattcag cccgctcgtt gttctgcctt ctgtgtctcc gtcctttttt
      841 cggttcttcg ctcaaagcat aggcacctgg ccccgacggc aaccttcctg tgactttcca
      901 cagggggaag aagggcttgc tgtatttctg cggaaagaag gtattgaccc gctgatccgt
      961 tgaggcgaag atgcggcagc gttggcacgg agcctgctac tcaagtctgt aacggattga
     1021 acgatgaggg gcgacctatg aaagtcttga ttgcgaccga tggatcgaaa tacgggaagt
     1081 gggcgacgga atgggtggcg cggatgccgt tcgccgagaa gccggatgtg agcgtcctgc
     1141 acgtgacgga tgtcgaggcc ctgcgcgcgc cgttcatgtt tcagccggtg gtgatcggca
     1201 atgagccgtt tattcaggaa gaaatcaagc ggattgaagc ccgtggaaag acggccatgg
     1261 ccgaggccaa ggctcagatg gcgtcgctca agttaaaagg caagctggtg tctgagcgcg
     1321 gggcagccgg ctcgaccatt ctggatcgtg cgccgaagcg tgatggactg gtggcgatcg
     1381 ggagccgggg gttggatgcg ctcgaccggt tcatgctcgg gagtgtctcg acccaggtga
     1441 cgttgcatgc gccctgctcg gttctcattg tgaaggaaga accgcgcccg ctgagccgga
     1501 ttctcttcgc agccgacggg tccaaggcct cggagaaggc gttgcggttc ctgctgacca
     1561 aactgcaccc ggaagagcgg gaaggcctgg agccgatcga tgtggtggtg atgcacgcca
     1621 tgccgttcct gaagtatccg gaactcaaag aggccggcgc ccgcctggtc gagcaatgcg
     1681 cgaacaagct gctgaaggcc gggtatgtgg ttgatgaagt ggtccagctt gggaaaccgg
     1741 ccgatgagat cttgaaagtg gcgacgaaga agaaagtcga tctgatcgtg accggggcca
     1801 aaggcatggg ggccgtcgcc aggttcctgc tgggcagtgt ttcgaccaag gttgtgcagc
     1861 acagtacgtg ctcggtgctc gtggttcgtt aggcaggctg ttgacaaagg ctgccagcgg
     1921 cgttctctcg gcgttcaaca gcctgcggca gattctggga acgtccgtgg ctctgctggt
     1981 tcctcccggg gcaactaacc cgcattattc atgacggttc gtcgtgaaat cgccgagtca
     2041 cgtggcgaga ccggcgcgcg gagccgggag gacccgaggc gtactcgtgc agtacgttga
     2101 gggtccgagg ggcgagcccg ccggccgaag gcccgtcgca gcagtgaatc ggcgattgca
     2161 gcagaagtgc tcatgaataa tgcgggctaa gaccgctgtg gcatttcacc ccgctctcag
     2221 ggccggtagc gtagcaaaac gctgctaccg gcatcccatt cctctcgaaa tccggactct
     2281 ctcacggcat ggtccctgct gttctcagga acagcagggt cgcgcaaggc agatgatcga
     2341 cgcatgacag acacagcggg ggttttcagg cggaattcag agggacagca tcggcgatcc
     2401 ggtgactggc gttctcttgc gggacgatgc ctggtgatcg ggctggtact gatatcggca
     2461 gcagcctgcg atcgggatgc gcccgtggcg ccgtcggccg gcaagccggt gaacagagcc
     2521 gccaacggga tggtgcagct gaccgatcct gaaattgcca gggccggcat cgaggtgatg
     2581 acggtcaaga aagagccgtt taccttccat cgcgaatttc ccgcgaccgt gcaagccaac
     2641 gagaatgaac tggcagaggt cacgaccctt attcgaggga gagtcgtcga ggtgctggtc
     2701 gatgtgggga aggatgtgaa gaagggtgag cggctggcgt tgctcgacag taccgatctc
     2761 gggatggccg aaggagcgta cctcaagtcg gcggcaaggc aacacgaagc gcagctggcc
     2821 tttgagcggg cggaaaatct ccatgagcat cgtgcgatca gtctggccga actgcaacgc
     2881 cgggaagcgg aaatgaaaac cgcgcgcgcc gatgcgcggg aggcatcgca tcggctggcg
     2941 ctccttggcc tgccggccca ggaaatcaaa cggctcgaac gcgagcagac gatccggtct
     3001 gatgtggcga ttcgagcgcc ctttgcaggc cgtgtgatca tgcgtaatat cacgcgcggg
     3061 gaggtggtgg agacctcgcg gaactgtttc accatcgccg acctgtccga tgtctgggtg
     3121 gtggccaacg tgccggaaaa ggacgtgcgc tttattcgtc cgaaggaccg ggttcatgtg
     3181 atggttccgg cctatccgca tgggctgttc tccggcaccg tgacctacat cagcgatgtc
     3241 ctcgacccat cgaccaggac gatgcgtgtc cgtgtcaccg tgctcaatcc cgatcggacc
     3301 ttgaaaccgg agatgttcgc catggtccgt gtctttgcgt cccctcaacc cgaggcgctg
     3361 gcggtgccgc tcacggctgt gcaacaggac ggcacgggca aattactgtt cgtgcggcaa
     3421 gcggacaatc gattcgaatc gcgccgggtt ctcctgggag acgagctgga cggcaagacc
     3481 atcgtcctgg aggggctgca cgagggtgaa gacgtggtgg tcaaagggtc cttcgcaatc
     3541 aagtcggagt tcgatatcca caaaatcgag cccacgccat gattgcctca ctgttggagt
     3601 tctcgctccg ccaatcgatc ctcgtcatcg gactggcctg catgttttcc gtggccggcc
     3661 tgttcgcctt tcaatccatt cccatcgatg cctatcccga cgtgaccaat gtgcaggtgc
     3721 aggtgttgac ggaagcgcct ggcctctctc ccgttgaagt ggaacgcttc atcacctatc
     3781 cgatcgaact gcagatgaac gggctgccgg gcctgacgga aatccgctcg ttgtccaagt
     3841 tcgcgctctc tcagctgacg gtggtgttcg aggatgatgt ggatgtgtac tttgcccgcc
     3901 agttggtgtt ggagcggatc atggcggtca aggagcggtt gccggcgggt atcgatccgg
     3961 tcctggcgcc ggtcacgact ggactgggag agatctacca atactatctc gaaggcccgc
     4021 aagcccatgc gaccgatacg gcgctcgtag aggccgagtt gacgaatcag cggacggtgc
     4081 aggattgggt actccgcccc ttgctgaaga gtgtgccggg cgtgatcgat gtgaacggcc
     4141 tgggcggatt cgtgaagcag ttccaagtgc tggtggatcc cgacaagttg cgcaaataca
     4201 gcctgacgct gcacgacatc tatgaggcgg tgcagaagaa caatgccaat tccggcggca
     4261 atgtgctgga acggcatgcg gaacgcgcca ttgtgcgggg actgggactc atcaaaaccc
     4321 tgcccgacat cgagcgtatc gtcgtgaagg aagcgggcgg cacacccgtg ttcgtccgtg
     4381 atgtggcaga ggttcgtatc ggtcatgcgg tgcgccatgg cgcggccgtg ttgaacggcg
     4441 agcgggaggt ggtggcgggc acggtgctga tgctgcgcgg aggcaatgca cgtgaagtcg
     4501 tccaagccgt gaaacgccgg gtcgacacga ttcagcagga tgggttgttg tcggaaggcc
     4561 tcaagatcat cccgttttat gaccggatcg agctcgtgac agcggccatc aataccgtgc
     4621 gcgatgcctt gatcgagggc atcgtcctgg tgaccatcgt atttttccta tttctgggcc
     4681 atgtgcggag cgcgatcgtc gtcactgtgt ctctgttggt cacgccactg atgacgttcc
     4741 tggtgatgca acgcgtgggg ctttccgcca acctcatgac cctgggtggc ttggcgatcg
     4801 ggatcggtga aattgccgac ggatcgttgg tcgtggtgga gaatgtctat cgccatctct
     4861 ccgacaatca tctgcatcag cggtcccggc ttgaggtgat tttccgggcc accagcgagg
     4921 tagggcggcc gatcgtgttc ggcatcctga tcatcagcgt cgtcttcctt cccctcatga
     4981 ctctgcacgg gatggaaggg aaaatgtttg cccccctggc ctatacgctc gtgatttcgc
     5041 tgctgatgtc ggtggtcgtc accttgaccc tctcgccggt tctggcgtcc ctgatcctgc
     5101 gcggcgacca tccggaggag acccggctca cccggtggat gaaggcccgc tatcagccgg
     5161 tgctccgttg gacgctcgac catcgggtgc cggtgttgct gggatcgacg gcggtggtgc
     5221 tggcgagtct gaccctgctg ccgttcgtgg ggcgggaatt cattccgatt ctggaggagg
     5281 gcgcgttgac gccgcaaatc gtgcggctgc cgagtgtctc gttgccggag tcgatcgcga
     5341 ttgagaaaca aacgcagaaa gtcatgctgg agtttccgga agtgcagatg tcggtgagca
     5401 agatcggacg ccccgatatc gccgtcggtc cggaggagcc gaatgagagc gatccgatcg
     5461 tgacgctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc gcaacagtat
     5581 cggggccatc ggagatatcc gggtacgatc cgcatccggc gcgccgattc ccttgagcga
     5641 gctcgcgacc atcgagatgc gcgaaggccc cgcccgcatc agtcgtgagc aggcgaagcg
     5701 gcggatctac atcggtttca atgtggtggg gcgcgacatc ggcagcgtgg tggatgaagg
     5761 ccggaagctg ttggccgaac ggatccggtt gccgcaaggg tatacggtgg cgtggggcgg
     5821 ggcgttcgag aatatggagc gcgcgaatgc gcggttgctg ctggtggtgc cgatcacgct
     5881 cgggttggtc ttctttctgc tcttctgggc gtttcactcg ctgcggtatg cctccttgat
     5941 cattctgaat ctgccgtttg ccttgatcgg cggcgtcgta tcgttgtggc ttagcgatca
     6001 gtatctgagt gtgcccgcct caatcgggtt tatcgaactg ttcgggttgg cggtgggaaa
     6061 cggcatcgtg ctcgtctcct acatcaatca gttgcggaat gaggggcagc agacggaggc
     6121 ggcgatcatg acgggctgtg tcttacggct tcgcccggtc gtgatgacga tgatgacgac
     6181 gttgctcggc ctcctgccgc tggtcctggc ccaggggatc ggcgccgaag tgcagcggcc
     6241 gttggccacc gtggttgtcg gcggcctgtt cacctcgact gcattgacct tgctggtcct
     6301 cccggccctg taccggacct ttgccgaaca ggaggccgcg aaggaacatg ccccggagtg
     6361 ggtgtgaagg gagttcacga gaccatggag aatagacgac ccttttgggc ctgttctccg
     6421 gatgaggtgc ttcgcgactt gcgtgccgta ccggaggggt tgagcaccgt cgaggccgaa
     6481 cagcggcagg tcgtctccgc gtccgcccgg ctcacacccc agcgagatag tcagccgttc
     6541 cggctcttgc tggcccagtt ccgaagcccc atcatcctca tcctgctctt cgcttccggg
     6601 gtgtcgtttt ttctcgcaga acatagcgat gcgctcatca tcctggccat cattctggtc
     6661 agcgccctgt tgagtttctg gcaggaatac agcgcggccc gcgccgtggc cggcctgttg
     6721 gcgctcgtgc agatcacggc tcaaacatgg cgggacggcc ggttgcgcga ggtgcctgcc
     6781 gacgacatcg tgccgggcga tatcgtggag ctttcggctg ggtcgagttt acccggagac
     6841 gcgcggctgc tcgatgccaa ggatctgttc gtcgatgaag cgaccttgac gggggagacc
     6901 tatccggcag agaaatcgac aggaacactc acggacgcgg tcccctcgca gaagcggacg
     6961 aacagcctgt ttctgggcac ccatgtggtg agcgggcagg cgcgggccgt gatcgtggcg
     7021 gtgggcaagg acacggaatt cggccgcatt gcccatcgga tgcaggtgcg ggcgccggaa
     7081 acggaatttg agcggggcgt gcgccgattc ggatatctat tgctggaagt cacgctgctg
     7141 ctggtgttct cgatattcgc ggtgaacgtg tatttggcgc gcccggtcct tgagtccttt
     7201 ctgttttcca tggcgttggc cgtcggcctg acgccgcaac tgttgccggc catcatcagc
     7261 gtcaatctct cccacggcgc taagcgaatg gcgcagcaga aggtggtcgt caaacgcctg
     7321 acctcgattg aaaatttcgg cagtatgaac gtgttgtgct ccgacaaaac cggcaccctg
     7381 accatggggt ccatgcggct gcacgcggca ctggatcttc agggccggcc gagcgagaag
     7441 gtgttattcc tcgcccagct gaacgcgatg ttcgaaacgg gattctcgaa tccgctggat
     7501 gaagcgttgc gccgacaccg gtcggtcgat ctcaccggtt atcgcaagct ggaggaagaa
     7561 ccgtacgact tcgtccgcaa acggctctcg atcctcgtgg cgaccccgca gacgcacgtg
     7621 ttgatcacga aaggggccgt ggacagcatg ctgacggcct gtctcgatgc tgaacagtcg
     7681 gacggcaccg tggtgtcgat cgatgaggtc cgggattcca ttcgacagca ggttcgcgaa
     7741 ctgagtgcgc aaggatttcg cacgctgggg ctggcctgcc gtaacatggg cgccgttgat
     7801 cgtgtctcca aggaacatga aacggcgatg acctttctgg gtctggtggc ctttgccgat
     7861 cctccgaaac ccggcatggc cgagaccatt gcgacgctca aaggcctcgg ggtctcgttg
     7921 aaaatggtga ccggggatca ggcgctggtg gcggcgtatg tcgggcgggc ggttgggctg
     7981 gagaatccgc acctgcttac gggaactgat ctgcgcggca tgagcgacga cgcgctacgc
     8041 acgcgcgcaa acagcatcga tatcttcgcg gaaatcgaac ccaatcaaaa ggaacgcatc
     8101 atccgtgcgc tgcgcgcttc cggcaatgtg gttggatatc tcggcgacgg catcaacgat
     8161 gccccggctc tccacgcggc ggatgtcggc atttcagtcg atagcgccgt cgatgtcgcc
     8221 aaagaagcgg ccgatctcgt cctgttggag catgatttgg gcgtgctggt gcaaggcgtt
     8281 cgcgagggcc gcatgacctt cgccaatacg ttgaaatatg tgtttatggc caccagcgcg
     8341 aatttcggca acatgttcag catggccggg gcctcgctgt tcctgccgtt tctcccgctg
     8401 ctgccgaagc agatcctgct gacgaatgtc ctgaccgatg ttccggaaat gaccattgcc
     8461 accgaccggg tcgatcacga actgatcgag cggccccgcc gatgggatat tccgttcatc
     8521 cggcgattca tgctcacctt cggtctggtg agttccctgt tcgactacct cacgttcgga
     8581 gtcctcttgc tgctgttaca ctcaacggcc ggccaatttc gcaccggttg gtttgtcgag
     8641 tccgtgctgt cggcctcgtt gatcgcgctc gtcattcgca cccgccggcc ctgttatacc
     8701 agccggccct cttccgcgtt gttgatcacg acgctcctga cgggactggg gaccatgttg
     8761 ctgccggtga caccggtggg agcgtttctg ggattcgagc cgctgccacc gatcttctgg
     8821 gcggcgatgc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctccaatac
     8941 tccgtcgtat gatccgcagg tgctgctcga ttcacagccg gtgaccgtga atgtgatcga
     9001 ccctgccgac cattccgtgc aattccaaaa tcgaacctcg ctggaaatat tcagtgacat
     9061 ggccggctcc agatgtcatg cgaaaatcgc ggggaaggcg gctccctgtg agttttgccg
     9121 catgcctgag gcgttggcgc gcggaggcgt ggtgtccgag gaggtggaca tgcccgatgg
     9181 ccgccgtctc ctgatccatt gggccaaggc cccgacgacg gacggacggg tccatgtgat
     9241 tgaaacgatc gtggatgtga cgaaacgcaa gcaggacgag cagagcctgc ggcagtcgca
     9301 gaagatggag gcactcgggc ggctggcggc cggcatcgcg cacgatttta acaatctgct
     9361 catggtggtg atcggccatg cgcaacgggt ggcacagcag ctcggctcgc atccttgcca
     9421 tcatgaagtg gagatgatcg ggcaggcggg gagcagggcg gccgccttga cgaagaagct
     9481 gttgacgttc agccgacgcc aggtgctgga gcaacgggat gtgccgctca atacgttgat
     9541 tcgcgacatg gaagatattc tacgccgttt aatcggcgag cagattcaga cggtgattgt
     9601 gctcgatccc agagccgggc atgtgttggg cgatcccgtg cagatcgagc aggtgttgct
     9661 caatctggcc ctcaatgcgc gcgattccat ggcggaaggc gggattctca ccatcgagac
     9721 gggcaatgcc gatctggatg aggcgtacgt gcgtgttcac ccgggcgcgg ttcctggtcc
     9781 ctacgtcaaa ctcgtggtgg aggataccgg atgcgggatc gacgccgaga cgctcgccca
     9841 tatctttgaa ccgttcttca gcacgaagga gttcggcaaa ggcaccggct tggggttagc
     9901 caccgtctat ggcattgtga aggagagccg cggttatatc gatgtgacca gccagccggg
     9961 gcgcggctcg cggtttacgg tgatgtggcc tcgggtgctg acgcaaacag gagaagccga
    10021 gccggtggcg gcgtctcgcg cggttccggc cgcttgcgcg actatcctgc ttgtggaaga
    10081 cgatgagggc attcggcgtc tcgtctcagc cgtgctccgg gaccaggggt atgaggtgtt
    10141 ggcggccacc gacggcgtcg aggccctgca aatgttgcag ttgcgaaaag ggggctgcga
    10201 cctcctgatc acggatgtga tcctgccgag aatgaaggcg gccgtgctgg cacagggggc
    10261 gaggacgatg tttccgaaca tcaaagtgct ctatatttcc ggttattccg gcgacatgct
    10321 ggtctcgcac ggggtcgatg cgcaagccgc ctatttgcag aaaccgtttc tcccccaggc
    10381 cattatcgaa aaggtggccg aactcctgcc cgcgcgcccg cagtagcgcg ggccgacgta
    10441 cgatgtgcct gattggatca atcctgcctg tatgcagccc gcttcccgat ggccgccggt
    10501 ggttccgtta cgatcctgcc tgtaggcata agggttgggt aaccgactgg tcaaatcgac
    10561 gcggatgact gcttgacgcc ggagcggagg agtcggcaca ctcccgacca ttgcggatct
    10621 cggtccttag acctgagaag cgtggtaagg agaggcgatt gctcgcgtca tggaccatcc
    10681 tcgtgctgag cggagccatg atcgtcatgg cggggacgaa actctcgcgc tgcggcgacc
    10741 agatagcggc gttgacaggc cttgggcgac tgtgggtcgg cgtcgtgttg atggcggctg
    10801 cgacgtcgct gcccgaggta ttcacgaccg tcagcgccgg tttgctcgat gcgccggacc
    10861 tggccgccgg agatctcttc ggcgcggtgc ttgccaacat gttcaccctt ggcctcatcg
    10921 accttatcca tcgcgacaag cgcgtctggg agcaggcggc tttcgagcat acgctcgtgg
    10981 ctgcgctggc gatgctcctc actggattag ccgcgtttct ggtattgttt cggagtggga
    11041 cgggtgcttc ggcaatcgaa gggggaagcg ctcttctctt tctggcctat gttttcggca
    11101 tgcggatggt gtttcggcag gaagacgtca agcggcgcca gcgggcacag caactccttg
    11161 ccgaggcgga ggctgacgac gaccacacgc gagagtcagt ccggcgtgac ggactgcgcc
    11221 gcgccgccat cggattcgtc gccgctacgg tggtcctgat catcgccgcc ccactgcttg
    11281 ccaagtcagc caaggatatc gcggatcaat cgggagtcag cacgaccttc gtcgggacgt
    11341 cgctcgttgc ggtggtgacg acattgcctg agttggtcac gtctctggcc gccgtccggc
    11401 tcggggcctt cgatcttgca gtgggcaatc tcttcggcag caatgccttc aatatggcgg
    11461 ccttcttgtt taccgacctg gcatacgccg gtgggccatt actcggtgcg atcgactcca
    11521 ctcatgcgct gaccgccctt tggggcattc tgatgatgaa tgtagggctg atggggattg
    11581 tgtatcgagt ggagaagcgg tattggctca ttgaaccgga tagcctggtg atgatcctcg
    11641 ggtatctgct cggcctctgg ctattgttcc gatgatgcct gccgtgtccc gagcaggatg
    11701 cggaaaaagt ccgccagcgg cgttctcgcg ggacactgcc gcctcactat ctcggcgacg
    11761 ttcacaagcg tgacgcgcgt tattcagcgc gtcgtgaacc tcagaggctc accgtacggc
    11821 tacgggacaa gagcctgtct tggcaggatc ggggtgggtg ggtgagtgag aatgaacgat
    11881 tcgcctcttc gctcgctacg gccttgttgg acggcctttt tgcgcatcct gcaaggatgt
    11941 gtccagtcac gtcacgggcg gctatgtcca ggagacggag gggagggaga tgactcgttc
    12001 gctctctgcg cttgactgtg cgtccaggag cagatcgtac acaattttta cacgcgcccg
    12061 tacatcctgg gcgagcgaca ggaattcgac tccgaacgaa tccttgccgg tccaggtgac
    12121 gacggcgggt ttgatttcga tgggttcccg gcgatcgggc agtgagatgc gcagcgtcaa
    12181 tcgcattccg caatgcacct tggcgtcagg ctgttcgatc cggcacccgc ccggagagaa
    12241 gtctcgcacg acgccctggc tggtcccgcg aggcccctga agctcaatgg gccggttcat
    12301 ctccactcgt gtgttcttcc gtttcgacat cgatcgccct ttccgcgctg cgcctctgcg
    12361 tgacaggatc aagcccgtgc tgttcggaaa caaccctgca acaggaacgg cgtgtgagac
    12421 atgcaccgac cccgtccgat gcgggcttca gtctgttggc gctccagacc tccgttcaaa
    12481 tagcgggttg catgagggac tccgccaagt ccctgaatgt cggatccagc atgaatcgcc
    12541 tgaatgaaac atcctgtcgg ggatccggta tgcggtcacc cgtctctcgc tgacgatcgt
    12601 tgcggaaggc cgcgtgaagg gactgcatgg ccttctcccg gtcgttcatt cccgcgtagg
    12661 tgcgggcgag gtggtaatga aacagtgagt tggcaggatc tcgtgagaga ccatagtcca
    12721 acgtagtctt ggcgcgtgcc aggtcaccgg tcatgccgta agccaccccc aggttatgaa
    12781 cgagcaggcg ccagacggcc ttgccgaggg tcgagtgtgc tcgttccaac gtgatcgcct
    12841 gttcatagtg cgggatggct tgcctgaatt gcccttgaag gtagggtgcg ctgcctgctc
    12901 gaaagtgatc gaggctgccg gccagggggg cggggacgat gtcgaggctc tgcagcaccg
    12961 ctcgcaatcg tgaatcatct ccggtcgtga aaccggtttt ggcaacatga atgtctgcgt
    13021 agacgttgtc tttacggacg caggcaaaca ggtggagttg atctaccgcg gttcctttgg
    13081 tctctggcag cagatactcg aggacaggca cctgcctgat ttcgtatcgc gtgatgtcct
    13141 gctggggttg ttccggtgat gtccgcgcga tgcgttccag atgggcaaga cagccttgtt
    13201 ccgtagcctg accggacacc gcttctaacg tgaccgagag atgcatcgct gcggcgctgt
    13261 tggtggcgaa gacatatcgc cgtccatccg gcttgacccc gtcgatctgc acacggtaac
    13321 cggtcatgtc gaggctcacg gcccacggct gttccacgag ggcgatgggt gcnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgcatgtc gcgcgggcgt gattggcgaa
    13501 tcgggcctgt ccccctcaac ggctgttggg tatcggcttt gcattgtgtg aggcaaggat
    13561 gtatctgtat cgtgggccgg tcatgccggc cgcagaacgg aaggagcaag aatgcagcag
    13621 cgaaccacgg ggctactggg gtttcggcga cggtggcgag tcgcctgctt attggcggca
    13681 agtctcatgc tggcggggcc gtcggcgcat gccaccgtac aggtgttgcc tgagatgacg
    13741 ctgaagcgtg aggggcggac cattaccgga gccaaggcga ttcgcgccga tcagacgatg
    13801 caggacattc tgacggcctt cgatcgtgcg gagcacgcgc tggaacggca ggatctcagc
    13861 gcgctgatgc agttttatgc gtccggctac aactaccacg gcttacatgt ggcggacgtg
    13921 gagcgcatct ggggcgaggt cttcgagcac tatcgcgcgt tatcctcgac gcacctgttt
    13981 tccgagatga aggtggtgca gaccgaagaa gggacccggg tggagttgac gtgcaccggc
    14041 ggtctttatg gtacagaggc gcagtcaggc gcccgcatca cgctggatag ttggttccag
    14101 gaggttcact atctggtgcg ggacaagaac ggatggcggt ttctcgggaa tcgaggggat
    14161 gcgccgcgtt cggcgccctt ctcctcgtcc ccgcaccacc ctctgttctg agcgggagtg
    14221 attaggctgc cgccgcggat gcggctgtgg cagcagtgcc gagcagtttc ttgacgctgg
    14281 catgggccag gaccacgtca ggtgtccgtt gcagcgcttc atagtagctg cgttcaaaac
    14341 catcgccctc ctcggaggga cgcagcatcg tgcgcgcttc cgacaggagg tcctgtgccg
    14401 cttccagggt cgtgtcgtct tgatcttcga ggacgacatc gatgcggacg acgaggtgtg
    14461 acaaggggtg aagccaggcg aaccaggggt cgttcatgac cagctgcagc agttgccccg
    14521 ccgaggagac gcgcccgtag atgcgttcat aggtgacttg ttcggagacg atgagggact
    14581 tgtgcaggcc aagcaggccg tggcggaggt ccttgaggct gcggcgcaaa ctgtccggaa
    14641 tcggattcgg agccgaccag gaagaacgtg tcatatacca ccaatcctaa cagatccaca
    14701 gcttcaagcg acggttgttc ccaccacagg aatgacgccc gatccagggc cgatgctccg
    14761 tccttcgtgg atactttatc ggcgtgtttt cgcaaggatt gagtcttcgg cacggggagg
    14821 gccacatttt tttgtaaggc cgcgtcgtag acgtgagnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnaat gaactgccgg aagccccgcc cccttcccct tggacgatcc
    15001 ttggggcgca gttcaccagc ctgattgtct gggtgctgat cggcgctgcg atcgtttccg
    15061 gcctgctcgg ggaatgggtc gataccggtg ccatcctcgc catcgtcctg ctgaacggat
    15121 ttctgggttt cgttcaagag tatcgagccg aacagtcgct ggcggcatta aaaaccctgg
    15181 cggtgaccta cgcgcgggta attcgcggcg gctcgcggcg tcagctgtct tccaccgaat
    15241 tggttcccgg cgacatcatc gacgtagagg cgggggatca catcccggcg gatgcgcgcc
    15301 tcattcaagc cgcggcgttg cgcacgcagg aggccgcgct gaccggggaa tccacgccgg
    15361 tcgataagtc aggcggggtg ctgcctgaca acgacctgcc cctggccgat cggcggaaca
    15421 tgctgtttct ggggaccacc gtcacgggag ggaagggccg tgcgctcatc gtcgcaaccg
    15481 gtcgtggaac cgagctggga cggatcgcca cgttgatgac gtcggtgcct gtcgagccga
    15541 ccccgctcca gcggcggttg gagcagttcg gtcatgtgct gctgctgctg tcgctcggga
    15601 tcgtcgtcgt tgtgttcgtg ctgggcttgt ggcgaggcga gccggtcttc gacatgtttc
    15661 tcacggccgt gagtctggcg gttgcggcca tcccggaagg tctgcccgcg attgtgacga
    15721 cgaccctagc acttggagtc atgcgcatgg tgacgcgtca tgcgttgatc cgtcgattgc
    15781 cggccgtgga gaccctgggg gcggcgacgg tgatctgtac ggacaagacc gggaccctga
    15841 cgaagaacga aatgacggtg acgcgcctgg ccctcgatgg acaggtgtat gaggtgaccg
    15901 gagaagggta tgggccggag ggtgacatta tcggtggcga tccgcgcgag ggaggcttgc
    15961 agcagcttct gttgagcgcc ctgttgtgca acgacgcgac gctcaaggaa gtcgatggca
    16021 gttggaaagt ggtgggcgat cccacggaag gggcgttact cgttgcgggc ggcaaggccg
    16081 gatggcggaa agaagacctg gagcgggcgc atccgctggt gggggaaatt cccttcgatt
    16141 ccgaacgcaa gatgatgacg gtggttcgac gatcgggcgc agacatcgtg gcctatgtga
    16201 agggagcccc ggatattctg ctcggccgtt gtcgcgactt tctatcggtc gaggggcagg
    16261 tgcagccgct caccgattcg atgagagagt ccattctgtc tatcaaccgg cagtttgccg
    16321 agcaagccct ccgtgtcgtc gccctggcac ggcggacact cgatcaagag ccgtccactt
    16381 acgactccga gaccattgag cggcagttgg tctttctcgg gttagccgcc atgaaagatc
    16441 ccttgcgtcc cgaagccaag gtggccgtcg agctctgccg gtcggcgggt at
//
LOCUS       DAOQRM010000048        16404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2625_length_16404_cov_15.474463, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000048 DAOQRM010000000
VERSION     DAOQRM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2625_length_16404_cov_15.474463"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(448..1341)
                     /locus_tag="PKK81_05815"
     CDS             complement(448..1341)
                     /locus_tag="PKK81_05815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV32421.1"
                     /translation="MPRVSVIIPAYNSMQWVSAAIDSVLNQGYRDYEVVVIDDGSTDQ
                     TADVLESYGRKIRCIYQANAGVSNARNRGLSVSTGEFIAYLDADDMWYSQKLECQIAF
                     LDAHPQCGLVHSDVTVIDEQEKVIRARFNVETARPVPSGYCLQDLLRRCHIQVPSVIE
                     RRECIEEVGGFDEQFMATEDYLHWIMVSLKGWEFGYINEPLAKYRWRGGSVSSNKRLF
                     LEEYGKMFGVLLPALYENRPQAPDLAIIRKRFLQVERELAYMDCIEGHLSSARQRLMR
                     LIQRAPFRYNLYWDLAKTYLR"
     gene            complement(1673..2785)
                     /locus_tag="PKK81_05820"
     CDS             complement(1673..2785)
                     /locus_tag="PKK81_05820"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV32422.1"
                     /translation="MISVFGSCVGQEEIDEIRTSFEKQWLGIGLKTRSFESLFAHRLG
                     LKDFVFLDNGSNSLYMAVKLLNLSPGSEVILPSFTWVACAHAIILAGGIPVFCDVDVN
                     TQNVTADTIAPHISSKTRAIMVVHYAGKPVRMEPVLSFGQPVIEDAAQAVDSKLGNKY
                     CGSIGTIGVYSFDPVKNITTGEGGGITTRDSELMTRARQLRYCGIGRAGFEAAAMKER
                     WWEYDIVDVFPKLLPNDIAASIGLAQLRKIDKFQTIRKKIWNIYQAQLADFDWLIRPQ
                     DPASDEQHSYFTYSIRVAKGKRDQLAKYLYSRGIYTTVRFHPLHLNPIYKSSYRLPIC
                     EQLNDEILSLPLHPNLSDSDIDAILLSLQKFNEQYM"
     gene            complement(3462..4235)
                     /locus_tag="PKK81_05825"
     CDS             complement(3462..4235)
                     /locus_tag="PKK81_05825"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNV32423.1"
                     /translation="MDHEKAVRQELRVVSHNLGGQSDDGGRVTATWAIPLALAGVLVA
                     GEPASADSEIYGYVGPNGVFEMTNVPTDQRFRSAESQVRRLSHRASAEEVEEAVKRYA
                     FQFQLHPALLLAVIKAESDFNPTVISRSGAVGLMQLIPETAIRHGVLNLYDTDDNIRG
                     GARHLRYLLDRFNGNVRLAVAAYNAGERRVERYRAIPPYPETRDYVRKVMIYYKSFRG
                     DYQASPGKAVLLAMYHQPLQLEPLPVSTDVRNSRPAVRK"
     gene            4870..5802
                     /locus_tag="PKK81_05830"
     CDS             4870..5802
                     /locus_tag="PKK81_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HNV32424.1"
                     /translation="MIKLLLVEDNPVDAQLTRDLLAEWPLDQFEITHAPILADGLTRL
                     SRDRFDVVLLDLSLPDTHGLSTVTQVLATSPGVPVVVLSGHDDHPLALKALQHGAQDY
                     LVKGEGGTDFLARSILYAIERKRAQERLTYLAQHDQLTGLINRALFRDRLIHAMARSK
                     RKDHPLAIMLLDLDRFKAVNDTLGHDVGDQLLKLVATRLLECVREVDTVARMGGDEFT
                     AILEGISGVADVLVVAKRIVESISTPFEIGPHRISIGVSIGITLYPLDDEDIDELLRH
                     ADKAMYAAKQQGGSRFHFHSTTGQPPSGTPAPVQ"
     gene            complement(5882..6715)
                     /locus_tag="PKK81_05835"
     CDS             complement(5882..6715)
                     /locus_tag="PKK81_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNV32425.1"
                     /translation="MERPQESGAVGGRREPLSEPPPNPKGGRTLIVDARDPHAYIRPS
                     AALKEAGETDQVFIRPGVYEDKVFVADRPVLLIGAGRDHVQIYSRRGGPLYLQRVPSG
                     MISGITFRYVGSDQHSAINVLDSVCTITQCRATEGVLSGVVIYGPEARATFVENEVCY
                     NRESGIFVFAGAQPRVAQNQCYGNHHFGLAVRDPGSHPECVRNVCHSNMLSGILLFHH
                     AEALLLDNTCRDNQHWGLVMTPDTHPTPPREDLVTSNVLLPNPRGSLVVTEQPLADIG
                     R"
     gene            7043..7564
                     /gene="ybgF"
                     /locus_tag="PKK81_05840"
     CDS             7043..7564
                     /gene="ybgF"
                     /locus_tag="PKK81_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNV32426.1"
                     /translation="MTRTHTKLGPVRGICLILALIGNLTTVPVLFAASPQPPDDAHRL
                     YDRVMEEFRHKDYAAALAGFRFFLELHGQSSLSANAQYWKGECQYRMGRYKDALDSFY
                     NLISDYPMSQKLAASTLKIGQIYTKQGDREKAQMMFERVTDQYPDSTEAEVARKALEA
                     AAAKGEPVAAEPS"
     gene            complement(7615..8133)
                     /locus_tag="PKK81_05845"
     CDS             complement(7615..8133)
                     /locus_tag="PKK81_05845"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNV32427.1"
                     /translation="MELLYPILGIGALVFLGSLAWESFGRRRSPGASTAEAPAGMSFK
                     AKPLLTDQEVQLYNLLRLAVEDRYLLFTQIPLWSLLDLRLPSGTPPAEMLRELALKRA
                     TFVLVHPGSRLVEKVVQVERPVPDDAPDGSHDSLFESALRAAGVQLVRLSPLHAYTVP
                     GLVTVLDLADPD"
     gene            complement(8149..9333)
                     /locus_tag="PKK81_05850"
     CDS             complement(8149..9333)
                     /locus_tag="PKK81_05850"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNV32428.1"
                     /translation="MKVVGLMSGTSADGVDAALVSIVRRGGTPRITPLAFASLPYPRS
                     LQQRIVELSLHGRVDDICHLNTYLGELFAKAALRVIQQSKCRPADIDLIGSHGQTIHH
                     LPKGRREPGVGLVRSTLQIGEPAVIAERTGITTVANFRPRDMAAGGEGAPLVPYAHAV
                     AFGHARRGRLVVNIGGISNVTYLPPGGDVADLRAFDTGPGNMVLDAIVHETSKGQSAY
                     DAGGRRARKGTVNRPLLTELMAHPFLTRRPPKSTGREEFGAPFVRSVLVKQRQARLSV
                     EDLLATCAAWTAEAIGSSRRWVTGEIHDVIVGGGGVYNRAIMASLRQVFAPTSVLTFD
                     ECGWSSKAFEATAFALLAHDTYHGQCTNVPQVTGAHHPVLLGAVVPGSPHMLTKWARL
                     AR"
     gene            9496..10422
                     /locus_tag="PKK81_05855"
     CDS             9496..10422
                     /locus_tag="PKK81_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNV32429.1"
                     /translation="MFRLIALAMFLAFAMPCEWGSSATAFAASAAEPLRVVVTLPVLK
                     DWVQQIGGSYVHVNSLMTGYESEHTYSPKPSDLVAVRKAALLFEVGAGLEVWVASLVK
                     NAGNGSLRVVTTSKDIELIQDHPEPAGTSHSHEHAAGNPHVWLDPAAAATMVQHISDA
                     MAAADPAHATDYRTNTATYLQALTRVQDESLARLRSLPSRAIIVHHPAWPYFARRYDL
                     HIAGTIQTQPGGEPSARHLHTLIETIKRDHVRVIISEVQLNQKVPQLLARETGARIAV
                     LTTLPGGVPGTETYLDMLRYNVLQLAQALEQT"
     gene            10483..11262
                     /locus_tag="PKK81_05860"
     CDS             10483..11262
                     /locus_tag="PKK81_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNV32430.1"
                     /translation="MTHPIIRFDHATFGFPGIVALEDISLTIPESEFVGVIGPNGSGK
                     TTLCRAVLGLMAPLSGTLRVLDCACEELRCHHRALIGYLPQKGMIDRNFPVTVLEAAM
                     MGRYGALGLFRRPSQKDRDIARQALAQVGMDHHRDSALGALSGGQQQRVFIARALAQQ
                     PRILLLDEPTTGLDLTAQHSVVELIQQLHHELKLTILMITHDINMIRSRVDRLVLLKT
                     KLFAEGPPQEVLKPEILSQVYGKELVITDKDFVLVEDYHHH"
     gene            11570..12364
                     /locus_tag="PKK81_05865"
     CDS             11570..12364
                     /locus_tag="PKK81_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNV32431.1"
                     /translation="MQRSLLAAALVGAVCSVIGVFVVLRGLAFAGAGTAHAAFAGVTL
                     AYLLGLPPLSLAIVFGLATVWITGWVEEKGRMKLDVSIGILYTATMALAILFLGLMKT
                     YNPEVYGYLFGSVLSVTTEELMTIGGLSVVVLGTILLLSKELYFIAFDQEMAAASGVP
                     ARQIFYLLLTLVALTVVIALKTVGAILVFAMILIPASTAYQLTHSLTQMTLYSMLIGI
                     FCAVSGVLLSYAFDLPSGPSIVLLATSLFFLAVCCSPKRLHRIQPT"
     gene            complement(12471..12914)
                     /locus_tag="PKK81_05870"
     CDS             complement(12471..12914)
                     /locus_tag="PKK81_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV32432.1"
                     /translation="MPPALKTDQHPITFSDRNDFDAAQLIHLYRQAPWAKHRALEQAQ
                     AMLAKTDVVISAWDGTRLVGFGRVLTDYVFRASIWDVIVDREYQKRKIGTEIVRRILD
                     HPTLQHVELFWLCTRMPGFYERLGFSAKEQTGMVWSRKKSEPQAS"
     gene            12978..13157
                     /locus_tag="PKK81_05875"
     CDS             12978..13157
                     /locus_tag="PKK81_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32433.1"
                     /translation="MGTQVRSCPKCFQLMWLKQDHYDVLDEETVRAKCPHCGSTVRFQ
                     LVTAGANAAGPKMGH"
     gene            complement(13216..13680)
                     /locus_tag="PKK81_05880"
     CDS             complement(13216..13680)
                     /locus_tag="PKK81_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32434.1"
                     /translation="MPNMTVGESEERRTMETSAVLDRIGELIAQLKGYASKLPPVVHL
                     ASVPTGVKPKPEAVEVFEQALLRFREQSGRSPFKSLQDYLIESLEAFEVGNVLGVVQP
                     LLAVLDQVERMHRDREIKVGPAEEKRMGEYRAALVKILPGSQPELEGAGRGM"
     gene            complement(13718..14683)
                     /locus_tag="PKK81_05885"
     CDS             complement(13718..14683)
                     /locus_tag="PKK81_05885"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNV32435.1"
                     /translation="MNRISSTPVEIVVTGGESSKRIDVFLANRDPTFSRSALQRLIGE
                     GRILINGQTIRPSQKIKPGDRIRLEVPRPEPLDLKPEAIPFDILHEDADLLVLHKPAG
                     LVVHPAPGNWSGTLVNALLHHFATSGVTPSHIGGKERPGLVHRLDKETSGVMVIAKTD
                     QAHRALAAQFKLHTITRVYEALIWGVPKKGHGLIELAIGRDTKERKKFSARTTKPKPS
                     ATDYQVDERYGKIAAHVRLSPRTGRTHQLRVHLTSIDHPILGDKTYGGAKVMSVAGVP
                     IPRVMLHARTLGFTHPTGGEYHQFDVPCPPDMEQVQDLLRAATAH"
     gene            15067..15552
                     /locus_tag="PKK81_05890"
     CDS             15067..15552
                     /locus_tag="PKK81_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32436.1"
                     /translation="MAADFIHRILRSLQGTFVPTPVAQPAIVDNLTEQPTPPPVRERR
                     IDTRFAVRTPCVYELVEGHGSDASTILGKAYSLNVSSDGILLLLDQKPQSRQLLNIRN
                     PALQRQRSLTLFEVRWSTHLPVGTTHPRYLVGCRLAFGRFPYFLVQRQHLDRDISGLS
                     L"
     gene            complement(15535..>16404)
                     /locus_tag="PKK81_05895"
     CDS             complement(15535..>16404)
                     /locus_tag="PKK81_05895"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNV32437.1"
                     /translation="REPKLSRAALQRMITAGWVRVNSLIAKSSQRIKAGDVIVFDAPQ
                     AAPLRIDGETKQLEILFEDHACLVLNKPVGIVVHPSPGHWSNTLLNALLDHCARGGAV
                     ATPGVVHRLDKDTSGVMVVAKTEEAHRKLSVQFERHSITRHYEALVVGVPAKSEGRIE
                     AALGPDRHHPKRTSTQTLQPKRAVTDYRVEEAFGEAAARIVLSPHTGRTHQIRAHLQS
                     INHPILGDQVYGGERVGAIAGYEIPRVMLHARRLGFTHPVTGMYGEWTAATPSDFDRV
                     RGVLCAGRLERKT"
BASE COUNT         3506 a         5037 c         4427 g         3434 t
ORIGIN      
        1 ctcataaccc aaaggtcgtc ggttcaaatc cggcccccgc aaccaacctt ctctcctctc
       61 tttcaaccac atacagcagt ttcagtccag acatcggcac tcgcacgagc agccatggac
      121 tgctccccta gagtgagacg caacggattg gcgcacttag caccagacgg atggtgaaga
      181 aacgcgcggg ggaatttgca gtacctttcg gcaggtggca gtggagcggc ccacgccgta
      241 cgacgctcgc agggaactcg acgtcagtct aaagggactt tgagcgctga catcagccag
      301 gaatcatgtt gaaccagcag gaaggggtgc gaggtttacc tggaatggct tgcccgctta
      361 ccagcaagga ctgtcccgat atgcctccaa cggcttcagt gtgtgcccga atgtgatcca
      421 aatagaagta gctttgtttc ggtcgtacta gcgcaagtag gtctttgcca ggtcccaata
      481 gaggttatat cggaacggtg ctcgctgtat cagtctcata agtcgctgcc tcgcgctact
      541 cagatgccct tctatgcaat ccatatatgc caattcgcgt tcaacctgca gaaatctctt
      601 gcgtatgatg gccaaatccg gtgcttgggg acgattctcg tataaagcgg gcagtagtac
      661 accaaacatc ttcccatact cctccaggaa gagtcgcttg ttggacgaaa cgcttccgcc
      721 tcgccaacga tacttcgcta acggctcatt gatgtatcca aattcccatc ccttcagtga
      781 caccataatc caatgcaaat aatcttctgt tgccataaat tgctcatcaa acccaccaac
      841 ctcttcgata cactcccgtc gttcaatgac cgatggaacc tggatgtgac agcgtcgcag
      901 aaggtcttgc agacaatagc ctgagggaac gggcctagcg gtttccacat taaagcgcgc
      961 acggatgacc ttctcctgtt catcaataac ggttacatcg ctgtgcacaa ggccgcattg
     1021 gggatgggca tccaagaacg cgatttgaca ttcgagcttc tgcgaatacc acatatcatc
     1081 ggcgtccaga tacgcgataa attcgccggt cgatacgctc aggccgcggt ttctagcgtt
     1141 ggatacacca gcattggctt gataaataca tcgtattttt ctgccgtagg actcaagaac
     1201 gtctgccgtc tgatcggttg acccatcgtc aatcacaacc acttcgtagt cccgataacc
     1261 ctggttcagg acgctgtcga tggcagcaga gacccactgc atagaattat aggcggggat
     1321 gatgacggat acacgtggca tgcgggcgtt ccctgaacaa tgctctgcaa cgcacgatgc
     1381 gcagatcagg gaagaactac tctgatcccc gcgaacgttg caattgtccc ctggagtgct
     1441 ttgtcacaag aataactgcc ttgtgctcac tgccctcaac atgtccggga atggcctgcg
     1501 aatacggcgc acgatcctct cggctgacgg cctctttggg tcacacagct gcttatgcgc
     1561 cgacacatct tttatcgcaa atgtgacctt cagcaataac cacgattacc taccagtgcc
     1621 tttgaagaac gcaattattc cgtggataca gtgatcacat cggacgctgt attcacatgt
     1681 actgttcatt aaatttttgc aaacttaata aaatcgcatc aatgtcgcta tcagaaaggt
     1741 ttgggtgaag tggaagacta agtatttcat cgttaagctg ctcacaaatg ggaagcctgt
     1801 aagatgattt ataaatagga ttcagatgca atggatggaa tcgaacagtt gtatagatac
     1861 cgcgggaata gaggtattta gccaattgat cgcgctttcc tttggcaacc cgaatggagt
     1921 aagtgaaata ggaatgctgt tcatctgaag cagggtcttg aggtcttatc agccagtcga
     1981 aatcagctaa ttgagcttga tatatattcc aaattttctt acggatcgtt tgaaatttat
     2041 ctatcttgcg tagctgcgcc agtccgattg aagccgcaat gtcattggga agaagtttag
     2101 gaaaaacatc tacgatgtca tattcccacc agcgctcttt cattgccgca gcctcaaatc
     2161 ctgcgcgtcc aataccgcaa taccgcaact gtcgggcccg agtcattaat tcggaatctc
     2221 tggttgtaat tcctcctccc tcacctgttg ttatattttt gacaggatca aaactataga
     2281 cgcctattgt tccaatagag ccgcaatatt tattgcctag tttggaatct acggcttgag
     2341 ccgcatcttc tatcactggt tgtccaaacg ataacactgg ctccatacgg accggtttgc
     2401 ctgcataatg tacaaccata atagccctgg tctttgagga aatatgcggg gcaattgtgt
     2461 cagcagtcac gttttgagta ttcacgtcta catcgcaaaa tacgggaata cctccagcga
     2521 ggatgatagc gtgggcgcat gcgacccatg taaaagaggg cagtatgact tccgatccag
     2581 gggataggtt taatagtttt acagccatgt ataaactatt ggaaccgttg tccagaaaca
     2641 cgaaatcctt gagccccaac ctgtgagcga agagagattc aaatgatctt gttttcagac
     2701 cgattccgag ccattgcttt tcaaagctgg tgcgtatctc gtcaatttcc tcttgtccta
     2761 cgcatgaacc gaatactgag atcatagtaa ggcccctatt caaggacata ctgaggatcg
     2821 gatgagccgc ggcggctgga aattacagtg tcagtcggta ataacgtgac cccgccgcac
     2881 aattctcgcc ttcacggcat caatcatgtc gcgaagtgct atggctagat caccgtgccc
     2941 ctattcagtc tcgtccgccg cgatttgcat tgctatcgag cacaaaccgt cgtttatttc
     3001 ggcgatgtgc gacttcagca aagctctccc ttgcccctcg tttgccctat caaaaccgtc
     3061 accacatgtg acgcatcata gccgaaccag ttcattactt catggcccat gtgatgacgg
     3121 acttgtgcga tgatgcggtc gcacatggtt acggttgagc gtgaccgagc gcgcagcatt
     3181 tgccgaatga agggcgccct attaccctga tgaaaccacc caagcttgca cggcaaagaa
     3241 ggccgggagt gttgaagatt gaacaggcaa gagagccacg ctggtcgtgg ccacccagca
     3301 ctggtggctg ttgaccaacg ccacgcattc ggtctgctaa atgtgcgcta cgcctcgtaa
     3361 ccctatcttg aggatgtgat gtatcggccg accgaatggt cggcctggtt atttgtccgc
     3421 ctcgtctggg aacgttagag aagatgagat actcggcccc cctatttcct gaccgcaggt
     3481 cgtgagtttc gcacatcagt agagacaggc agtggctcaa gttgcagcgg ctggtgatac
     3541 atagcgagca acacggcctt cccggggctc gcctggtagt caccacggaa gctcttgtaa
     3601 tagatcatca ccttcctgac atagtcgcga gtttccggat agggcggaat tgcgcgatac
     3661 cgctccacgc gccgttcacc tgcattatac gcagcgaccg ccaaccgcac gttcccgtta
     3721 aatcgatcca gtaagtaccg caggtgccgc gcgccgcctc gaatgttgtc gtcggtgtca
     3781 tagagattga gcaccccgtg cctgatggcg gtttcgggta tcagctgcat cagtccaacc
     3841 gccccggatc gtgaaatgac ggtgggatta aagtccgact ccgccttgat caccgccagc
     3901 agtaaggccg gatgaagttg aaactggaag gcgtagcgct tgacggcttc ctccacttcc
     3961 tccgccgagg cgcgatgcga caggcggcgc acctgcgatt ccgccgagcg aaaccgttgg
     4021 tcggtcggaa cattcgtcat ttcgaacacc ccgttcggcc ccacatagcc atagatctcg
     4081 ctgtctgcag aggcgggctc cccggccacc agcacccccg caagcgccag aggaatcgcc
     4141 cacgtggcag tcacacgccc gccatcgtca ctctggccgc caaggttatg gctcactact
     4201 cgcaactcct gccggacagc tttttcgtga tccatctctt cctcctcttg ttcccgcagc
     4261 aacccatagc cggggaatcc acagcgtcgc gaaccccagg ctgaagcgca gctttcatgc
     4321 catcgtcctg acgtgtacaa aagcgggcag ctccaataac ttcgtacggt tagggcgttc
     4381 acaaggagtc tctcccctgg tgagcgggga gaggaaccga accgttttgt gccaccattt
     4441 ggccggattt tgttctttgt ttcgttgctg ttcgttacca acatgacgcg ccatcgcaga
     4501 tttacgggca gggcatgcat ctgtagaccc gaactgagga ggtttcttca catgaggcat
     4561 tgtcggtcga caccaggacg cagggcatgc cggcagatca cgcacgatca tcgtgaagaa
     4621 gtgcgctctt gtttgaagct tcagagtgac gtgctagaat cgatttcggg ctggtccctc
     4681 aatcacgcac tgcctgcagc cacagggaga ttcccgcact gaatacgccc ctcggccgtc
     4741 accaccattt gagatgaaaa ttccacactc gatagctccg gcgcgcagca tcacggtgat
     4801 ctgtcagcca tccgcttgtg cgcagcacat cgcctcgcca gattgtctta cggacgcgag
     4861 cacgcaccga tgatcaaact gctgttggtc gaagacaatc ccgtcgatgc ccagctcacc
     4921 cgagacctcc tggccgaatg gccgctcgac cagttcgaga tcacccatgc ccccattctg
     4981 gcggacggct tgaccagatt aagccgggac cggtttgatg tggtactcct cgatctctcc
     5041 cttcccgaca cccatggctt gagtaccgtg acccaggtct tagcgaccag tccaggcgtg
     5101 ccggtggtgg tgctgagcgg gcacgacgat cacccgctgg ccctcaaagc gctgcaacac
     5161 ggggcgcagg attatctggt gaagggagaa ggcggaaccg actttcttgc acgctcgatt
     5221 ctctatgcca tcgaacgcaa gcgggcacag gaacgcctga cctatcttgc ccagcacgat
     5281 cagcttaccg gcctgatcaa ccgcgccctc ttccgcgacc gcttaatcca tgccatggcg
     5341 cgcagcaaac ggaaagatca ccccctggcg atcatgctgc tcgacctgga tcgattcaaa
     5401 gccgtcaatg atacgctggg gcacgatgtc ggcgatcagt tgctgaaact cgtggcgacc
     5461 cgactgctgg agtgcgttcg tgaagtggat accgtcgcgc gcatgggcgg cgatgagttc
     5521 accgccatcc tggaaggtat ttccggcgtc gccgatgtac tcgtggtggc caaaagaatc
     5581 gtggagtcga tcagcacccc gttcgagatc ggaccgcacc ggatctcgat cggcgtgagc
     5641 atcggcatca cgctctaccc gttagacgat gaggatatcg acgaacttct cagacatgcc
     5701 gataaggcca tgtatgccgc caagcagcaa ggcgggagcc gatttcactt tcattctacg
     5761 accggacaac ctccctccgg tacccccgcc ccggtacagt agcgagaagc cgcttcggga
     5821 gccgttcttt cttcaaagat cgaccgccgc gacaagcacc ccctccgcac ggcatgtctg
     5881 cttagcgacc gatatcggcg agcggctgct ctgtcacgac caacgaccct cggggattcg
     5941 gcagcaacac attagaggtg accaagtcct cgcgaggcgg agtcggatgc gtatccgggg
     6001 tcatgaccaa accccagtgc tggttgtccc gacaggtgtt gtcgagcagg agcgcctccg
     6061 catgatggaa caggaggatc ccgctgagca tgttgctgtg acagacgttg cgcacacatt
     6121 ccggatggct gccgggatct cgcacggcca agccgaaatg atgattccca taacactggt
     6181 tttgcgccac acgcggttgt gcgcccgcaa aaacaaaaat gcctgactcg cggttgtaac
     6241 agacctcatt ctcgacaaac gtcgcacggg cctcaggacc ataaatcacc actcctgaca
     6301 acacgccttc cgtcgcccgg cattgcgtaa tcgtgcagac tgagtccagg acgttgatcg
     6361 cggaatgttg atcactgccc acatagcgga aggtaatccc gctgatcatg cccgagggga
     6421 cccgctggag atagagcggc cctccgcgac ggctgtaaat ctgcacgtga tcacgcccgg
     6481 cgccgatgag gagcacagga cggtccgcga caaagacttt gtcttcatac actcccggtc
     6541 gaatgaagac ctgatcagtc tcaccggcct ctttcagtgc cgcgctcggc ctgatgtagg
     6601 cgtggggatc gcgggcgtcg acaatcaacg tccggccccc cttcggattg ggaggtggct
     6661 cgctgagcgg ctcccttcgg ccaccgaccg cgcctgactc ctgtggtctt tccataccga
     6721 ctttctcgat cctctacaga ctcggaatca gcacacccgc cgccatggcc ggctgcctgc
     6781 taaccgacaa caagtgaaaa catgcgatct gtcaaccggc cagagcagcg cctcatggct
     6841 ggcgctttcc gtacaatact gtctgctttc cgcccctgtt gttccggcac atccaggcca
     6901 ctccgccttc aattccacgc gaactataca atttcacaac gggttcactt gtcgagcctc
     6961 gctggtgcgg ttctttcata tcggaaagca caacgaccgt gtgaacccag actcgtccaa
     7021 cagggaagaa agaggggcac tcatgaccag gactcatacc aagctcggtc ccgtgcgagg
     7081 catctgtctc atcttggcgt tgatcggcaa cctcacgacc gttccagttc tgttcgcagc
     7141 ttcacctcaa ccgccggatg acgcacaccg cctctacgat cgcgtgatgg aagagttccg
     7201 ccacaaggac tatgccgcag ccctcgccgg gtttcgcttc ttcctcgaac tccacgggca
     7261 atcgtccctg tccgccaacg ctcaatactg gaaaggcgag tgccagtacc gtatggggcg
     7321 ctacaaagac gcgctggact cgttctacaa cctcatctcc gattacccga tgagccagaa
     7381 gctcgccgcc tcgacgctca agatcgggca gatctacacg aagcaaggcg accgcgaaaa
     7441 agcgcagatg atgttcgagc gggtgacgga ccagtacccc gatagcacag aggccgaggt
     7501 ggctcgcaaa gccttggagg ccgctgccgc aaagggtgaa ccggtcgctg ccgagccaag
     7561 ctgatccgga ttgcgcgccc tttcggcaca ggcccgttga cagtcgaacc ggcgttagtc
     7621 cggatcggca agatccaaca cggtaacgag accgggaacc gtataggcat gcagcgggct
     7681 cagccgcacg agttgcaccc ctgccgcacg aagcgccgac tcaaagagtg aatcgtgcga
     7741 cccgtccggg gcatcatccg gcaccggccg ctcgacttgt accaccttct cgaccaaacg
     7801 gctgcccgga tgcaccagca cgaaggtggc gcgtttgagc gccagttccc gcaacatctc
     7861 ggcaggcggc gttcccgagg gaagacgcaa atccaacaag ctccaaagag gaatctgcgt
     7921 gaacagcagg taccggtctt ccacggcgag gcgcagcaga ttgtagagct gcacttcctg
     7981 gtcggtcagc aacggcttgg ccttaaaact cataccggca ggagcctctg ccgtggacgc
     8041 gccaggtgaa cggcgccgcc cgaaactctc ccaggccagg cttcccagaa acaccagcgc
     8101 cccgatacca aggataggat acagcaattc catgacaagc caatcggatc agcgggcgag
     8161 acgcgcccat ttcgtcagca tgtgagggga tcccggaacc acagcgccaa ggagcacggg
     8221 gtgatgggca cccgtcacct gcggcacgtt ggtgcattgt ccgtgatagg tgtcgtgagc
     8281 cagcagggca aacgccgtcg cttcaaaggc cttgctgctc catccacatt cgtcaaacgt
     8341 caacacagat gtcggcgcaa atacctgccg gagagaggcc ataatggcgc gattgtacac
     8401 accgccccct ccgacgatca catcatggat ctcgccggtg acccaccgtc gggacgaacc
     8461 gatggcctcc gccgtccagg ccgcacaggt cgccagcaga tcctcgacgg acagacgcgc
     8521 ctgacgttgt ttcacgagaa cacttcgaac aaagggcgca ccgaactctt cccgacccgt
     8581 cgacttgggc ggacgccgcg tcaggaacgg atgggccatc aactcggtga gcagaggccg
     8641 gttcactgtc cccttacgtg cgcgccgccc gccggcgtcg taggcactct gccccttcga
     8701 cgtttcgtgc acaatcgcat ccagcaccat attcccgggg ccggtgtcga acgcgcggag
     8761 gtcggcgaca tcaccgcccg gcggcagata ggtcacgtta ctgatcccgc cgatattcac
     8821 caccagacga ccccgacgcg catggccaaa ggccaccgca tgggcatagg ggaccagcgg
     8881 agcgccctcg ccccccgccg ccatatcgcg cggcctgaaa ttcgcgacgg tggtgatgcc
     8941 tgtgcgttcc gcgatcacag ccggctcacc gatctgcagg gtggaccgga ccaacccgac
     9001 accaggctca cgtcgcccct tcggcaaatg gtgaatcgtt tggccgtgcg atccgatcaa
     9061 atcaatatcg gccgggcggc atttcgattg ctgaatgacc cgcagagccg ccttggcgaa
     9121 cagttccccg agatacgtgt tcaggtggca gatgtcgtcc acgcggccat ggagcgagag
     9181 ttcaacaatt cgttgctgga gcgagcgcgg gtacggcagg gaggcaaatg ccaggggggt
     9241 gatccgcggc gttccgccgc gccggacgat gctgaccagc gccgcgtcca caccatccgc
     9301 cgatgtcccg gacatgagtc cgacaacttt catcgcgccc cttctccggc acacaaccgg
     9361 gagatttctt cctgttcggt tcgctacgct aatcgaaacc ccggcaacgg gtcaagcatc
     9421 gcgcgtatca tccgcgcaca atctccaccg gccttcgcgt tgactttgca gaaatcgggt
     9481 gttaccgtcc gccggatgtt tcgcctgatc gccttggcca tgtttctcgc atttgcgatg
     9541 ccctgtgaat ggggctcctc cgctacagct tttgctgcat ccgctgccga accgcttcgt
     9601 gttgttgtga cgctcccggt cctgaaggac tgggtgcaac aaatcggcgg ttcgtatgtg
     9661 catgtgaact cgctcatgac cgggtatgag agtgaacata cctattcgcc gaaaccaagc
     9721 gatcttgtcg cggttcgcaa agccgcgctc ttgttcgaag tcggtgccgg actcgaagtc
     9781 tgggtggcct cactggtgaa gaacgccggc aatggctcgt tgcgtgtcgt gacgacctcc
     9841 aaagacatcg agctgatcca ggatcacccc gagccggcag ggacgagcca ttcgcacgag
     9901 cacgcggcgg gtaatccgca tgtctggctg gatccggcgg ccgcagccac gatggtccaa
     9961 cacatttccg atgccatggc cgccgcagac ccggcccatg caaccgacta tcggaccaac
    10021 accgcgacat acctccaggc actcacgcgc gtgcaggacg agtcgctggc acggctccgc
    10081 tcgttgccga gccgcgcgat aatcgtccac cacccggcct ggccctattt tgcccgacgc
    10141 tacgatctgc acatcgccgg cacgattcag acacaaccgg ggggagaacc gtccgcgcgg
    10201 catctacaca cattgatcga aaccatcaaa cgagaccacg tccgcgtgat tatctccgag
    10261 gtacaactga atcagaaggt tccacagttg ctggcgcggg aaaccggtgc ccgcatcgcc
    10321 gtgttgacga ccttgcccgg aggcgtaccg gggaccgaga cctacctcga catgctccgc
    10381 tataatgtgc tccaattggc ccaggcgctt gagcaaacct gaccgccgca gccacccgga
    10441 catcgaccga caaaccagca ttttcaacgt acaccgccgc gcatgaccca tcccattatt
    10501 cgtttcgacc atgccacctt cggttttccc ggtatcgtgg ccctggagga catctccctg
    10561 accattccgg aatcggaatt tgtcggggtg atcggcccca acggctccgg gaaaacgaca
    10621 ctctgtcgtg ccgtgctcgg cctgatggcc ccgctgagcg gaaccctccg tgtcctcgac
    10681 tgtgcctgcg aagaactccg ctgtcaccat cgggcgctca tcggttatct gccgcaaaaa
    10741 ggcatgatcg accggaactt tcccgtgact gtactggaag cggccatgat gggacgatac
    10801 ggcgcgctcg gcctcttccg gcgaccgtca caaaaggatc gggacatcgc ccgtcaggcg
    10861 ctggcccagg tcggcatgga tcatcacaga gactccgcct taggtgccct ttccggcgga
    10921 cagcagcaac gtgtgttcat cgcacgggcg ctggcgcagc agccccggat tctcttgcta
    10981 gacgagccga cgaccgggtt ggacctcacc gcgcagcaca gtgtggtgga attgatccag
    11041 cagctacatc acgagctgaa gttgaccatt ctcatgatca ctcacgacat caacatgatc
    11101 cggtcgcggg tggaccgatt ggtgttgctc aagacgaagc tctttgccga agggccgccg
    11161 caagaggttc tgaagccgga aattctgagt caggtatacg ggaaagaact ggttattacg
    11221 gacaaggact tcgttctcgt cgaggattat catcaccact gaagcacata cgatgaatcg
    11281 atctggcgac ggtcctgatt cgagggccga gcccggatta ttcacgaata ccgtcgcgag
    11341 gcgttcgaga cggaagcccg ccggggcttc tcgcaagtga ggccgacgca ggcgtactgg
    11401 cagtccgtcg aggaggccga acgagaacag cctcgccggg cgagaggatc ggacgccgga
    11461 gcaggtattc atgaatagtc cgggctagac tcagtcatcg cactcattca ttcacgagat
    11521 tattcggtca ccacatatta cgatgctcga actgctcgcg tacgatttca tgcaacgctc
    11581 acttctggcc gccgcactgg tcggcgcggt ctgctccgtc atcggcgtgt tcgtcgtctt
    11641 acggggcctc gcgttcgcag gggccgggac cgcccatgct gcatttgccg gtgtcaccct
    11701 cgcctatctc ctcggccttc cgccgctcag tctcgccatt gtgttcggac tggcgacggt
    11761 ctggatcacg ggttgggtcg aggagaaggg gcgcatgaaa ctggacgtgt ccatcggcat
    11821 cctctacacc gccaccatgg cgctggccat tcttttcctc ggactcatga agacctataa
    11881 ccccgaggtc tacgggtacc tgttcggcag cgtgctttcc gtaaccacgg aagaactcat
    11941 gaccatcggc gggctcagcg tggtggtgct gggaactatc cttctgctct cgaaggaact
    12001 gtacttcatc gccttcgacc aggagatggc ggcagcgtcc ggcgtccccg cgcggcaaat
    12061 cttctacctc ctgctaacac tcgtggcgct caccgtggtg attgcgctga aaaccgtcgg
    12121 ggcgattctc gtgtttgcga tgattctgat ccccgcatcg accgcatacc agcttacgca
    12181 cagcctgact cagatgacgc tctactccat gctcatcggc atcttctgcg ccgtgagcgg
    12241 cgtactgctc tcctatgcct ttgatcttcc ctccggtccg tccattgtcc tcttggccac
    12301 cagccttttc tttctcgcgg tctgctgttc gcccaaacgg ttgcatcgaa ttcagccgac
    12361 atagaacaac ccggcaggac cgttcccctc acaggcaagg ataggcgaac cagggatcgt
    12421 cgacagggac tggatgagta cggaaaagaa aaccgttgga ggcggatgga ctatgaagct
    12481 tgcggctcag acttcttccg agaccagacc ataccggtct gctctttcgc gctgaacccg
    12541 agccgttcat aaaacccggg catgcgcgtg cagagccaaa acagttcaac gtgctgaagg
    12601 gtgggatggt cgagaatccg tcgaacgatc tcggtgccga tctttcgttt ctgatactcg
    12661 cgatcgacga tcacgtccca aatcgacgcc cggaatacgt agtcggtcag cacgcggccg
    12721 aagcccacca ggcgagtgcc atcccaggcc gaaatgacta catccgtctt cgccagcatc
    12781 gcctgcgcct gttcaagcgc acgatgcttg gcccagggag cctgccggta caaatgaatc
    12841 agttgtgcag cgtcgaaatc gttgcggtcg gagaatgtga tgggatgctg atcggtcttg
    12901 agagcgggag gcatcttgta ctaaattaat ccctccgaac ggggtgagtc ggttaaccgt
    12961 agtgtaaggg gaagaccatg ggaacgcaag tgcgaagctg tccgaagtgt tttcaattaa
    13021 tgtggctgaa gcaggatcat tatgatgtcc tcgacgagga gacggtccgc gcgaaatgtc
    13081 cgcactgtgg atcgacggtc cgcttccaac tggtcaccgc cggcgccaac gcggccggcc
    13141 cgaagatggg acattgaccg ttcccggcat gttgaacgga tgatcctgct tcagtgaaca
    13201 tgaccgctcc gagcgtcaca tgccccgtcc tgcgccctcc aactccggct gactgcccgg
    13261 caggatctta accagcgccg cgcgatactc ccccatccgc ttctcctcgg ctggaccgac
    13321 tttgatctcc ctgtcccgat gcatgcgctc cacctgatca aggacggcca gaagcggttg
    13381 aaccacgccc agcacattgc cgacctcgaa cgcttcgagc gactcgatca gataatcctg
    13441 cagactctta aacggagagc ggccgctttg ctcccgaaac cgcagaaggg cctgctcgaa
    13501 cacttccacc gcctcaggtt tcggtttaac cccggtcggc accgacgcga gatgtaccac
    13561 cggcggcaat tttgaggcgt accctttcag ctgcgcgatc aactcaccga tccgatcaag
    13621 cacggcactg gtttccatcg ttcgtcgctc ctcactctcg ccgaccgtca tgttcggcat
    13681 gttcttcaat ctggctcggg ccgatcgccc gggactccta atgcgccgtg gctgcgcgaa
    13741 ggagatcctg cacctgctcc atatcaggcg gacagggcac atcgaactga tggtactccc
    13801 cgccggtggg atgcgtaaat cccagcgtac gcgcgtggag catgacacgg ggaatcggca
    13861 cccccgccac cgacatcact ttggccccgc cgtatgtctt atcccccagg atgggatgat
    13921 cgatcgacgt gagatggacc cgcagctgat gggtgcgtcc ggttctgggg gagaggcgca
    13981 catgggccgc gatcttgccg taccgctcat caacctgata gtcggtcgcc gaaggtttcg
    14041 gtttggtcgt ccgcgccgaa aactttttcc gctctttggt atcgcgcccg atcgccagtt
    14101 caatgaggcc atgccccttt ttgggtacgc cccaaatcaa ggcttcgtac accctggtga
    14161 tcgtgtggag cttgaactgc gcggccagcg cgcgatgcgc ctggtccgtt ttcgcaatca
    14221 ccatgacgcc cgaggtttct ttgtccaatc ggtgaacgag tcctgggcgt tccttacctc
    14281 cgatatgcga cggggtgaca cccgaggtcg caaagtgatg caagagggcg ttcactaacg
    14341 tcccggacca attccccggc gccgggtgca ccacgaggcc ggccggctta tggagcacga
    14401 gtaagtccgc atcttcgtgc agaatatcaa aggggatggc ctccggtttg agatccagcg
    14461 gttcaggccg cggcacctcc agccggatgc ggtcacccgg tttgatcttc tgacttggcc
    14521 ggatcgtctg gccattgatg agaatgcgcc cttctccgat caagcgctgc aaggccgaac
    14581 gagagaaggt cgggtcgcgg ttggcaagga agacatcgat ccgcttggac gattctcctc
    14641 cggtcacgac aatttcaacc ggagtgctac tgatcctatt cacgtggtga ctgcctgaca
    14701 tcctgcgaca tgcgtgggca taggctgcca cagaggccgg tcaaacacaa ggtacccgcc
    14761 gctccatccc catcctctgc acacctcaca cacatgtcgt ccacaatcca tccacatgtg
    14821 gtgcggcaca cgcgcatcct tcagcgccgg cacgaatgta cgtttgcctg ttcatttctg
    14881 tcagtggtgc gttggatttg acaccatgat actgcgcatt ccgctgatgt cgcatgatgg
    14941 cacactcgca ctccagaaaa aattcatgtt gttagcatga tgcctgtctc tcccgctaca
    15001 ggcacctgac ttgcttattt attaagaagg tttggcgctt caacagttga agattgtgac
    15061 gatccaatgg ctgctgattt tatccacaga atcctcaggt cgctgcaagg aacgttcgtt
    15121 ccaacaccgg tcgctcagcc cgccattgtg gacaacctga ctgagcaacc gacgccaccc
    15181 cccgtgcgcg aacggcgcat cgacactcgg tttgccgtca ggactccttg cgtctacgaa
    15241 ctggtggagg gacatggctc cgacgcctcc acaatactcg ggaaggccta ttcgttgaat
    15301 gtcagttcgg acggcattct cctcttgctt gaccagaaac ctcagagcag acaattactc
    15361 aacattcgga atccggcact gcaacgacag cgctcactga cattgttcga ggtgcgctgg
    15421 tccactcacc tcccggtggg caccacccac ccgcgctacc tggtcggttg ccgcctcgca
    15481 ttcggccggt tcccctactt cctcgtccag cgccaacact tggaccgcga catctcaggt
    15541 ctttcgctct aatcgcccgg cgcatagcac tcctcgtacc ctgtcaaaat cggacggcgt
    15601 ggcagccgtc cactcgccat acatcccggt gacaggatgt gtgaacccca accgacgcgc
    15661 atgtagcatc actcggggaa tctcataccc tgctatcgca ccgacccgct cacccccata
    15721 gacctggtcc ccgaggatgg gatggttgat gctttgaaga tgggcccgta tctggtgcgt
    15781 acggcctgtg tgaggactga gcacgatgcg cgccgccgct tcgccaaagg cttcttcgac
    15841 tcgatagtct gtgaccgcac gcttcggctg aagcgtctgg gttgaggtgc gtttgggatg
    15901 gtgccgatcc ggaccaagcg cagcctcgat ccggccttcc gatttagccg gcacgcccac
    15961 gacgagcgct tcatagtgac gggtaatgga atgtcgttcg aactgcaccg acagttttcg
    16021 atgcgcctcc tctgtcttgg ccaccaccat cacgccagat gtatctttat ccaaacgatg
    16081 caccacgccg ggcgtcgcca ccgcacctcc gcgggcgcaa tggtctagta atgcattcag
    16141 cagcgtgttg gaccaatgtc caggggacgg gtggaccacg atcccgacgg gcttattcag
    16201 caccaggcac gcatgatcct caaacagaat ctccagctgt tttgtctcgc catcgatccg
    16261 taacggggca gcctgaggcg catcaaagac gatgacatct cccgctttga ttcgctgact
    16321 cgatttggcg atcagactat taacccgcac ccagcccgca gtaatcatcc gctgcaaggc
    16381 cgcgcgcgag agcttcggct cgcg
//
LOCUS       DAOQRM010000049        15908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2770_length_15908_cov_15.972119, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000049 DAOQRM010000000
VERSION     DAOQRM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15908
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2770_length_15908_cov_15.972119"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PKK81_05900"
     CDS             <1..820
                     /locus_tag="PKK81_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNV32438.1"
                     /translation="PWEFKRIWHLEPSLTDPDTVYAGAEDAAIFKSSDGGKTWNELPG
                     LRSAKGHLWQPGAGGMCLHTILLDQSQPDRMYIAISAAGAFRTDDGGKTWKPINRGLK
                     SQYELPDPDAEVGHCVHRIAMHPSRPNVLFMQKHWDVLRSDDAGDSWHEVSGNLPSDF
                     GFPIAVHAHEPNTVYVVPIKSDSEHYPPEGKLRVYRSKTGGHEWEALTKGLPQQDCYV
                     NILRDAMAVDQLEPCGLYFGTTGGQVYASRDGGDSWTAIVRDLPGVLSVEVQAL"
     gene            1060..>1310
                     /locus_tag="PKK81_05905"
     CDS             1060..>1310
                     /locus_tag="PKK81_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNV32439.1"
                     /translation="MIRVVLPAHLRTLARVTGEVALDVASPVTQRAVLDALEARYPML
                     RGTIRDHVTHKRRPFIRFFACEQDLSHESADVPLPDTVAS"
     assembly_gap    1311..1410
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1411..1740
                     /locus_tag="PKK81_05910"
     CDS             <1411..1740
                     /locus_tag="PKK81_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNV32440.1"
                     /translation="GLLACLLAMSAAMKLVGGREVIEGFARMGLPESLRVPLGVLELV
                     CVLVYVFPATSVMGAILLTGYVGGTIVTHLRIGEPVVFQIALGLLVWLGLYLRESRLK
                     SLLPLRT"
     gene            2022..2450
                     /locus_tag="PKK81_05915"
     CDS             2022..2450
                     /locus_tag="PKK81_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNV32441.1"
                     /translation="MRFIVFVKATKNSEAGVMPSQELLAAMMKYNEELVQAGVMLGGE
                     GLHPSSKGVRVKFSGSKRIVTDGPFVETNEIVAGYWLWQCKSKEEAIEWVKRCPSPMP
                     GEESEIEIRPLFEADDFGAELTPELREREEQLRAQAAGKK"
     gene            2645..3244
                     /locus_tag="PKK81_05920"
     CDS             2645..3244
                     /locus_tag="PKK81_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HNV32442.1"
                     /translation="MRYITVTLVALALVMTASFGEAKEKKHDKQMDPQAMMELWQKLA
                     QPGEPHKAFAGLAGTWTTTTKEWMEPGKPPTEASGTADMKMLLGGRFLYQEFNSQMMG
                     QPFSGIGIDAYDNVRKKYVTAWMDTMGTGIFIMEGTASADGKTITLKGSHPEPGGGQM
                     HHRAVWKLVDSNTQTFEMYGNHGHGKEEKMMEITYTRKP"
     gene            3381..3815
                     /locus_tag="PKK81_05925"
     CDS             3381..3815
                     /locus_tag="PKK81_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32443.1"
                     /translation="MQRTIGFLAMALILTSLSGCSYLFYPRAGDYATQAKGANGLETM
                     TNLTSMMESTASKAKGGKGVDMAFDDLHNQFHALSDSFCGVTDAQSKTPAYALAVTHK
                     KELSAIFKRLWKFKDDQPQRDQHLELLATELKELRETLRTIK"
     gene            4264..5460
                     /locus_tag="PKK81_05930"
     CDS             4264..5460
                     /locus_tag="PKK81_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV32444.1"
                     /translation="MARRSRFSTVAPMIYGCLALALLSSCKQEVGSSQAPPVPEVGIV
                     VATAQDVPDEPEFIGQSESSRPVEIRSQVTGILKEWFFKEGRDVKKGDRLYQIDPVPF
                     HAAMLSAKAKVAQAEARLVQAKQNLARVKPLLAEQAVSTKDVDDAVAEEMAAKANLEG
                     AKAELVKAKFDLDNTLIVAPISGMIERTRVYEGRLVSAQTDLLTIIQQVDPMYVIVSA
                     PESFLLKRQRDSTAKRIQHPGVYQLRGVLTFADGTTYGQEGVLDLLDVGLKTDTGSRQ
                     ARVVFPNHDRVLLPGQFVRVRFKGTVKTGAILVPQRAVQQGAKGSIVFVVGNEDKVEM
                     REVQATSWQGNQWIVEQGLHVGDRIIVEGLHKIAPGAPVKPVPLPAPAATTVPTAAKP
                     QPERAS"
     gene            5457..8636
                     /locus_tag="PKK81_05935"
     CDS             5457..8636
                     /locus_tag="PKK81_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNV32445.1"
                     /translation="MISHFFIDRPIFASVLSIVIMVVGLVALKALPVAQFPEITPPVV
                     QIEADYPGANAEIVADSVARPIEVQLPGIDNLLYYDSTSTNDGHMSIKLTFEIGTDVD
                     IAQVQTQNRVKLAEPQLPPEVVRQGISINKVSPDLLAVVALSSIDPTHDTVYLSNYAI
                     LRVLDNVKRLRGVGNAVVFGSQNYSMRLILDPIRMAQLSLTPTDIANVVREQNRDFPA
                     GTIGREPALKGTELTIPVITQGRLTEVKEFEELIVRAMPNGSMIHLKDVARVELGAQS
                     YTLEGRWNGKPNVFLLTFLSPGANALETVKRVRAELAEVSKSFPTGVSYDIPYDTTRF
                     IDVSIKEVVKTLAEAMVLVILVVYLFLQSWRATLIPGVAVPVSLVGTFAGMQALGFSI
                     NTLTLFGMVLAIGIVVDDAIVVVENCERHMTQGRLSAKNAAKRAMEEVTGPVIAIVLV
                     LCAVFVPVGFLGGITGELYKQFAITISIAVIISGFVALTLSPALCALVLKPGEEQHHG
                     IFGLFNRFFSWMQLRYISTVGLALTRRVLSVAVFAVLLIGVFMLFRVIPSSFLPEEDQ
                     GYFITIVQLPDGASKQRTDAVLSKIESYFLASPVIHSTDALSGQNFVFNTRGPNAATM
                     FVPLKHWDERSAPQHHVKALIGAAYGEFAKIPEALILAFNAPSIRGLGATGGFTLQLQ
                     DPSSGDFNKFSAAAQEFVGKARQNPAIGAIGTSFRVSAPRILATVNRERAKALGVPIS
                     EVFDTMQAYFGNFYINDFIKFGRVFRVQTEADAQYRSTPEDISKIYVRASGPQGTTMI
                     PLDTVVSTEFTSGPDPVTHFNGYNTALVLGAAAPGFSSGQVLDALEQVAKEVLVPQGY
                     GIDWSGISYQERMVGSQSLYAFAFGLLMVFLVLAAQYESWVVPFAVILAVPIGLFGAL
                     SAVWLKGMTNDIYFQIGLVTLIGLSAKNAILIVEFANKRFEEGHPLLEATIEAAKLRF
                     RPIVMTSMAFILGVVPLVIATGAGAASRNSIGTGVFGGMLAATFLAIFFVPLFFVLIR
                     SLSRRLKKETPPAQDAPDNPEKERDYQHA"
     gene            8629..10065
                     /locus_tag="PKK81_05940"
     CDS             8629..10065
                     /locus_tag="PKK81_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNV32446.1"
                     /translation="MRRLALVLSSILLTACAVGPDFTKPDATTPDAFRMAEAGGNAAS
                     IANTPWWELLKDQELQKLIRTALEENKDLQRAAAAVEEFQARLFIAKTDFAPQMNVTS
                     NAPLFGRKTNFLFPGFPNPFNYYLQGNLSWEIDIWGRIRRSNEAARGDLLSREESRRA
                     IVLQLVSGVAEAYFELLQFDMQLDIARRTLKSWEESVRIAQARLRQGMISKLDADQFE
                     AERANAAAKAAEFERQKVQKENQLSVLLGRNPGRIARGHSLTEQVMPPDVPPGLPSEL
                     LQRRPDILQAEQDLAAATARIGMAKADRFPKLSITGILGVASPHLSRLVANETAFGVA
                     GPGFAGPLLNAQILGFQQKAAEAQARQAVAQYEQSVLVAFKEVEDSLVAVSTVREQRT
                     AQLQQVDALRSALSLANLRYKGGLANYLDVLIAQRNLFEAELALMGTHRLHLVSIVQL
                     YKALGGGWTPEGQPPVQALPLVSTQETR"
     gene            complement(10235..10606)
                     /locus_tag="PKK81_05945"
     CDS             complement(10235..10606)
                     /locus_tag="PKK81_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32447.1"
                     /translation="MTSTAKTVVRDAAIVYGLTFAAGLCMAAAGITLENNSSTAYLCN
                     LLSGVLGFTLVGTRLSANRAEHLAWVAATLWTFNLTNIVLGLQTSSAWIHSGLTILLM
                     ASLGGSLAMILTLTSAADRRT"
     gene            complement(11197..11442)
                     /locus_tag="PKK81_05950"
     CDS             complement(11197..11442)
                     /locus_tag="PKK81_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32448.1"
                     /translation="MTCSRCNGCMVEDFLLDMEDSSGPMWLQAKRCMNCGNVAEKVLE
                     HNRQTQGAALHSSKSAPTIPNSEHAHFGSGGMAQLAA"
     gene            complement(11678..12040)
                     /locus_tag="PKK81_05955"
     CDS             complement(11678..12040)
                     /locus_tag="PKK81_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32449.1"
                     /translation="MTRSSHRRFVVHCILSAAAMGCAEKGRIIDSGLVGAPHPTSHSL
                     IDGDYLALKLQVPEDRRPVSVGLARVTWAQGNRFGVELLMMDADERARLNGFLEENLP
                     LELEFQDSHQELTITAAE"
     gene            complement(12378..13574)
                     /locus_tag="PKK81_05960"
     CDS             complement(12378..13574)
                     /locus_tag="PKK81_05960"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HNV32450.1"
                     /translation="MSKRLILCFDGMWDLPPDPSRQLPSSSTPNTQTLHNSHRHLSPA
                     TNVLRLYHSILPRTEDGRAQQKWYDSGADIPWFYRFRPGSFGYGVDQTILHAYAYLAA
                     TYEPGDELFVYGFSRGAYTARSLVGLLTTAGLIPAGLLHTDLVKRVREVVSHPSPSTT
                     TPTALRQSLHEMILEPSNQALDDAYRLYRKGHGDILAGMPTSARRRGTLEVPITLLGL
                     WETVGPLGIPTNALKWLNDHRYNFHDTELSSIVKQAYHALAIDEHRADHNATLWTSPA
                     CPGQTIEQRWFAGAHGDLGGAYPERDLADVALAWLQRHSMEKGLAIETRLVPLQPNAL
                     SPIHDSFSECFGRIRKWFHSRFYRPVMQTGTNTEVLDSSVHTRLIHTPHYRPKNEGLK
                     SVLHFE"
     gene            complement(13966..14529)
                     /locus_tag="PKK81_05965"
     CDS             complement(13966..14529)
                     /locus_tag="PKK81_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32451.1"
                     /translation="MRRPTRGLKYRLTIMLLGTYLVGAGCVHHIHVTSPSSDRAAAPI
                     PATVQLEVPFLAIEGADHMPGIPLLEWPSKDLQQTIVEYFTQRQTFTSIGTEAGDMRL
                     VVKAWLMLRAPDRYLYRVHLESDLAFSGQAPLKTYAAEGEALGSSVRWVTASDQEPIA
                     EATAQALHQLATQIEQDREFVIKATHR"
     gene            complement(14544..15029)
                     /locus_tag="PKK81_05970"
     CDS             complement(14544..15029)
                     /locus_tag="PKK81_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32452.1"
                     /translation="MAIRSRTASMWHGLVLLTACLTLAHCSHDIHEKRADTVKDHVEA
                     FYDHLTHDRVAAAVRENEAIEHLSSQLGDIISRRVNRPGTNQVDREWTDLRTANETAA
                     QNWLALGQYLSIKKQYAQSRATYQRVIDTYTGTTERTYREQAARAIRDLDILTPPPSS
                     H"
     gene            complement(15132..>15908)
                     /locus_tag="PKK81_05975"
     CDS             complement(15132..>15908)
                     /locus_tag="PKK81_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNV32453.1"
                     /translation="PTYAGGLGFGMKWDMGWMHDTLEYMALDPVYRKHHHRNLTFRML
                     YAFQENFLLPLSHDEVVHGKGSLLGKMPGDDWQKFANLRALFGYMYAQAAKKLIFMGG
                     EIGQWREWAHDDSIDWNLLQYQPHQGLQRWVADLNHLYRTEPALHEFDFDPRGFEWID
                     CQDVDAGVISLLRHGRTPQQHIAVVCNFTPVPRLQYRVGVPQGGYWKELLNSDASMYG
                     GTGLGNLGGLSAEPIPAHDRTHSLAMTLPPLSVLFFKAPS"
BASE COUNT         3233 a         4118 c         4957 g         3500 t
ORIGIN      
        1 gccgtgggaa ttcaaacgca tctggcacct agaaccctcg ctgaccgatc cggacacggt
       61 ctatgccggc gcggaggacg ccgcgatctt caaatcgtcg gatgggggaa aaacctggaa
      121 tgagctgccg gggctacgta gtgcgaaggg ccatctctgg cagcccggcg ccgggggtat
      181 gtgcctgcac actattctgt tggatcagag ccagcccgat cgcatgtaca tcgccatctc
      241 tgcggcgggc gcattccgca ccgatgatgg cggcaagacc tggaaaccga ttaatcgcgg
      301 cctcaagtcg caatatgagc tgccggatcc ggatgccgaa gtcggccact gtgtgcatcg
      361 cattgccatg catccgtcgc gtccgaacgt gctgttcatg cagaaacatt gggacgtcct
      421 tcgcagcgac gatgccggcg actcgtggca cgaggtcagc ggcaacctgc cgagcgattt
      481 cgggtttccg atcgcggtgc atgcccatga accgaatacg gtgtatgtgg tgccgatcaa
      541 gagcgattca gaacattatc cgccggaggg gaaactccgg gtgtatcgca gcaagaccgg
      601 cggccatgaa tgggaggcac tcacgaaggg tctgccgcag caggattgtt atgtgaacat
      661 tctgcgcgac gcgatggcgg tggatcagct tgagccctgc ggcctctatt tcgggacgac
      721 cggcgggcag gtctatgcgt cccgcgacgg gggtgacagt tggacggcga ttgtgcggga
      781 tttgccgggg gtcttatccg tagaggtgca agcgctctaa cgtctgctga aaatggccgc
      841 cagcggcgtt ctcagtcgcg accctcactg cgacgtaccc cgagggtacg cctcattcgc
      901 ctcgctccct gcggtctcgc tggacgacca ttttgagcag cgttatggag agagaagcca
      961 ggtacggctg ttaaacagcc ctgtgactgc cacgacggtt gagagttgag agaggattga
     1021 cggcccgttt ccccgcgaga aacgaacgat ggaggataca tgattcgtgt cgtattgccc
     1081 gcacatttgc ggacgttggc gcgtgtgacg ggtgaagtgg cgctggatgt ggcaagtccg
     1141 gtaacgcagc gggcggtgct cgatgcgctt gaggcgcggt accccatgtt gcggggaacc
     1201 attcgcgacc atgtcacgca caagcggcgg ccgttcattc ggttctttgc ctgcgagcag
     1261 gatctgtcgc acgagtcggc tgatgtcccc ctcccggaca cagtggcatc nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtctgcttg cttgtctgct ggcgatgagc
     1441 gcagcgatga agttggtcgg tggccgggag gtgatcgaag gctttgcccg gatgggactt
     1501 cccgagtctc tgcgcgtccc tcttggggtg ttggaattgg tctgtgtctt ggtctatgtt
     1561 tttccggcca catccgtcat gggcgccatt ctcctgaccg gctatgtcgg cggtacgatc
     1621 gtgacccatc tgcgaatcgg tgagccggtg gtgtttcaga ttgcgctggg tctcttggtc
     1681 tggttgggac tgtacttgcg cgagtctcga ctgaaatcgt tgctcccatt gagaacctag
     1741 cccggactat tcatgaatac ctgctccggc gtccgatcct gtcgcccggc gaggctgctc
     1801 tcgttcggcc tcctcgacgg actgtaggta cgcctgcgtc ggcctcatgt gcgagaagcc
     1861 tcggcgggtt tccgcctcga acgcctcgcg acggcattta taaataatcc gggctcgtgc
     1921 cctgtcgatt ttcgcctgtg agggcgacta atgggtagat ggcggccggt gcgggaacga
     1981 ggccgtcgca caaccatttc accagggaag gagcgcgtcc catgcgattt attgtattcg
     2041 tcaaagccac caaaaattca gaggcgggtg tgatgccgag ccaggagttg ctggccgcca
     2101 tgatgaaata taacgaggag ctggtacagg ccggagtgat gcttggaggg gaggggcttc
     2161 acccgagttc gaagggcgtc cgcgtgaagt tttcgggtag caagcgaatt gtcaccgacg
     2221 gaccgttcgt tgagacgaac gagatcgtcg ccggctattg gttatggcaa tgcaaatcca
     2281 aagaagaggc catcgagtgg gtcaaacgct gtcccagtcc gatgccaggc gaagaatctg
     2341 aaatcgagat tcgtcccttg ttcgaggccg acgattttgg agccgagttg actcctgagc
     2401 ttagagagcg ggaagagcag ctacgggcgc aagcagccgg caagaagtag gacagtcatg
     2461 ggggtctttt gcagcatgga taagatggtc gacaaggatt tcgaggatgg cctggcctcc
     2521 ctcaaggcgt tggccgggaa ataacagtgc caggggtctt gtcagacttg atctgatcgt
     2581 atgacgcgag catagttcta gaagaagata ctcaacacat ctcagtcaga ctcaggaggc
     2641 cattatgcga tacatcacgg tgacactggt tgcgcttgcg ttggtcatga cagcctcgtt
     2701 tggtgaggcc aaagaaaaga agcatgacaa acaaatggac ccgcaggcga tgatggaact
     2761 ctggcagaag ctggcccaac ccggtgagcc gcacaaggcg tttgccggct tagccggcac
     2821 ctggacgacg acgacgaagg aatggatgga acccgggaaa cctcctacgg aagcgagtgg
     2881 gacggctgac atgaagatgc tcttgggcgg acgttttctc tatcaggagt tcaacagcca
     2941 gatgatgggg cagccgtttt ccggaatcgg gatcgatgcc tatgacaacg tgcgcaaaaa
     3001 atatgtgacc gcctggatgg acaccatggg gaccggcatc ttcatcatgg aaggcacggc
     3061 aagtgcggac gggaagacga tcacgctgaa ggggtctcat cccgaaccgg gcggcgggca
     3121 gatgcatcat cgcgcagtct ggaagttggt ggacagcaat acgcagacgt tcgagatgta
     3181 cggaaaccac gggcacggaa aggaagaaaa aatgatggag atcacctata cccgtaagcc
     3241 ataggtgcac tctcctcaac cctgtgacgt gacgtgtgag tatcgagcgg cgtggactgt
     3301 gtggaggcat gaacacagtg ccctgggcgt ggccgccatc agtggccggt gagtcatcaa
     3361 tttttcgaag ggaggccgtt atgcaacgga cgatcggatt cctcgcgatg gcgctcattc
     3421 tgacaagctt gagcggctgc agttatctct tttacccgcg agccggagac tacgccacgc
     3481 aggcgaaagg ggcgaacggt ctcgagacca tgacgaacct caccagtatg atggagtcga
     3541 ccgcctccaa ggcgaagggt gggaagggcg tcgacatggc cttcgacgat ctccacaatc
     3601 agttccatgc cttgagtgat tcgttctgcg gtgtgacgga tgcgcaaagc aaaacaccgg
     3661 cctatgcgtt ggcggtgacg cataagaaag aacttagtgc gattttcaaa cggctgtgga
     3721 agttcaaaga tgatcagcct cagcgagacc aacaccttga gttgttggcc actgagttga
     3781 aagaactccg tgagacgttg cggacgatca agtagggcca gtcgtagagg atgatggcag
     3841 catggtctgc cgggcggagg ctagttgaat cacctttgat acgtgctgcg cgctctggca
     3901 tggtcaagtg aggaagatgt tggaatttac ctagcctagg acgtcagagt taagcatctt
     3961 cagtcaggga acatgagtgg gaagagggtg gcgaatccag gcgtgggaga ctgggatctc
     4021 gcgccccctt cccatgatgg cgtccccacc gaaccacgtg tcgtctcccc gctcggcgca
     4081 accttcaagt ctcgtgcagt ccattcttga actaagggaa gagcactgtg ccctgattgc
     4141 ttacgagtgt ctgccacgca gatgcgcgcc gaggctccgt tgcttacgca ggagccaacc
     4201 agtaagatag gcttccagta atatcccatg aactgcgtaa tcagatttta tcagggagcg
     4261 agcatggcca ggcgatcacg attttcaact gtagccccga tgatatacgg gtgcctggcg
     4321 ttggcgctgc tctcaagctg taagcaggaa gtcggttctt cccaggcccc tccggttcca
     4381 gaggtcggca ttgtcgtggc gacggcgcag gacgtgcccg atgagccgga gttcatcgga
     4441 cagtcggagt catcgcggcc ggtagaaatc agatcgcagg tgacagggat tctcaaagaa
     4501 tggttcttta aggaaggccg tgacgtcaag aaaggcgacc gcctctatca gatcgacccg
     4561 gtgccctttc atgccgcaat gttgagcgcg aaggcgaaag tcgctcaggc agaggcgcga
     4621 ctggtgcagg ccaagcagaa tctagcccgg gtgaagccct tgttggccga gcaggccgtg
     4681 agtacaaaag atgtcgatga tgcggtggcc gaggaaatgg cggcgaaagc gaacctcgaa
     4741 ggcgccaagg cggaactggt caaagccaag ttcgatctcg acaacacgct gatcgtggcg
     4801 cccatcagcg gcatgatcga gcggacgcgt gtatatgaag gacgacttgt gtcggcccag
     4861 accgatctcc tgacgatcat tcagcaggtc gatcccatgt atgtgatcgt cagcgcaccg
     4921 gaaagttttt tattgaagcg gcagcgcgac agtacggcca aacggattca gcaccccggc
     4981 gtctatcaat tgcgcggcgt cctgaccttt gcagacggga ccacgtacgg gcaggagggg
     5041 gtgttggacc tcctggatgt cggactcaaa accgatacgg gatcgcggca ggcccgggta
     5101 gtctttccca atcatgatcg cgtgctgctg cccgggcagt tcgtgcgggt ccgcttcaag
     5161 gggacggtca agaccggcgc gattctggtg ccgcagcgtg ctgtgcagca aggcgccaag
     5221 gggtccatcg tgtttgtcgt cgggaatgag gacaaggttg agatgcggga ggttcaggcg
     5281 accagctggc aaggcaacca atggattgtc gagcaagggt tgcatgtcgg ggaccgcatc
     5341 atcgtggaag gtctgcataa gattgcgccc ggcgcgccgg tgaagcctgt gccgcttcct
     5401 gctcctgccg cgacgaccgt tccgactgcc gccaagccgc agccggagcg cgcctcgtga
     5461 tctcccattt ttttatcgac cgtccgatct ttgcatcggt tctgtccatc gtgatcatgg
     5521 tggtcggcct ggtagcgctg aaggcgctgc cggtcgctca atttcctgaa atcacccctc
     5581 ccgtcgtcca gatcgaggcc gattacccgg gtgccaacgc ggagatcgtg gccgactccg
     5641 tggcgcgtcc cattgaggtt cagcttccgg gcatcgataa tctgctgtac tacgactcca
     5701 ccagcaccaa cgacgggcac atgtcgatca agctgacgtt tgagatcggc actgacgtgg
     5761 acatcgccca agtgcaaacg cagaatcggg tgaaactcgc ggaaccgcag ctgccgcctg
     5821 aggtcgtgcg ccaggggatc agcatcaata aggtttctcc cgatttgctg gcagtggtgg
     5881 cgctaagctc catcgatccg acgcatgaca cggtgtattt gtcgaactat gcgattctcc
     5941 gtgtgctcga caatgtgaaa cggttgcgcg gagtgggcaa tgcggtggtg ttcggctccc
     6001 agaactattc gatgcggctg attctggatc ccatccggat ggcccagctc agcttgacgc
     6061 cgacggacat cgccaacgtt gtgcgtgagc agaaccggga ttttccggcc ggaacgatcg
     6121 gccgtgagcc ggcgttgaag ggcaccgagc tcaccattcc cgtcatcacg cagggccgat
     6181 tgacggaggt gaaggagttc gaggagctga tcgtccgtgc catgccgaat ggatcgatga
     6241 tccacctcaa ggacgtagcc cgcgttgaac tgggtgcaca atcctatacg ctggaagggc
     6301 gttggaacgg caaaccgaac gtgtttttgc tgacgttcct gtcgcccggt gccaatgcgc
     6361 tcgaaacggt gaagcgggtt cgggctgaac tggccgaggt gtccaagagt tttcccacgg
     6421 gcgtgtccta cgatattcct tacgacacga cacgcttcat cgatgtctcc atcaaagaag
     6481 tggtgaagac gctggctgaa gcgatggtgc tcgtcattct ggtggtctat ctcttcctcc
     6541 agagttggcg cgcgaccttg attcccggcg tggcggtgcc ggtctccctc gtcggcacct
     6601 ttgccgggat gcaggccttg ggtttctcga tcaatacctt gaccctgttc ggcatggtgc
     6661 tggcgatcgg tatcgtggtg gatgatgcga ttgtcgtcgt ggaaaattgc gaacggcaca
     6721 tgacgcaggg gcggctttct gcgaagaatg ccgccaagcg ggcgatggaa gaggtgacgg
     6781 ggccggtcat tgcgattgtg ttggtgttgt gcgcggtgtt tgtgccggtc ggattcttgg
     6841 gtggtattac cggcgagttg tataagcagt tcgcgattac gatttcgatc gcagtcatta
     6901 tctccgggtt tgtcgcgttg accctcagtc ccgcgctctg tgctctcgtt ctgaagcccg
     6961 gtgaggagca gcaccatgga atttttggcc tcttcaatcg attcttttcg tggatgcagt
     7021 tgcggtatat ctcgacggtc ggccttgcgc tgactcgacg tgtcttgtcg gtggcggtgt
     7081 tcgcagtcct gttgatcggt gtgttcatgt tgttcagggt cattccgagc agtttcctgc
     7141 cggaggaaga ccagggctac ttcatcacca tcgtgcagct gcccgatggc gcctccaagc
     7201 agcggacgga tgcagtactg agcaagatcg agagctattt cctggccagc ccggtcatcc
     7261 attcaaccga tgccctgtcc ggtcagaact tcgtcttcaa tacgcggggg ccgaatgccg
     7321 ccacgatgtt cgtgccgttg aaacattggg acgagcgctc ggctccgcag catcatgtaa
     7381 aagcgcttat cggcgcagcc tatggagagt tcgccaagat cccggaagca ctcattctgg
     7441 ctttcaatgc gccttctatt cggggacttg gggctacggg tgggtttacc ctgcagcttc
     7501 aggatccgag cagcggggat ttcaataagt tctccgccgc cgctcaggag ttcgtcggca
     7561 aggcgcggca gaatccggcg atcggcgcga tcggcacgag ttttcgcgtc agtgcgccgc
     7621 gcattcttgc gacggtgaat cgcgagcggg cgaaggcgct gggtgtgccg atctcagaag
     7681 tgttcgatac gatgcaggcg tatttcggta atttctacat caacgacttc atcaagttcg
     7741 gtcgcgtctt ccgggtgcag accgaggccg atgcgcagta tcgatccacg ccggaggata
     7801 tttcgaagat ttatgtgcgg gcgagtggcc ctcagggtac caccatgatt ccattggaca
     7861 ccgtggtgag cacggagttc acgagcggtc ccgatcctgt gacgcacttc aatggctaca
     7921 ataccgcgct ggtgttgggg gcggcagccc ctggattcag ttcaggccag gtgctggatg
     7981 cgctcgaaca agtggcgaaa gaggtgctgg ttccgcaggg atacgggatc gattggagcg
     8041 gcatttccta tcaggagcgc atggtcggca gtcagtcgct ttatgcattc gcattcgggt
     8101 tgctgatggt gttcctggtg ctggctgccc agtatgaaag ctgggtggtg ccgtttgcgg
     8161 tgattctggc ggtgccgatc ggtctcttcg gcgcgctcag cgcagtttgg ctcaagggaa
     8221 tgaccaacga tatctacttc cagatcgggc tcgtgacact gatcggactc tcggcgaaaa
     8281 acgcgattct catcgtggaa tttgcgaaca aacggttcga ggaaggccat ccgttgctgg
     8341 aagcgacgat cgaggcggcg aagctccggt ttcgtccgat tgtcatgacg tcgatggcat
     8401 tcattctcgg cgtggtacct ctggtcattg ccacgggcgc cggcgctgcc agccgaaact
     8461 ccatcggcac cggggtgttc ggcgggatgc tggccgccac ctttctggct atctttttcg
     8521 tgccattatt ctttgtgctg atccgctcgt tgagtcgccg cttgaaaaag gagactcctc
     8581 cggcacagga tgcaccggac aatcccgaaa aggagcgcga ctatcaacat gcgtagactg
     8641 gccctggttc tttcctcgat ccttctgacg gcttgtgctg ttggcccaga cttcaccaaa
     8701 cctgacgcga cgactccgga tgccttccgc atggcggagg cgggtgggaa tgctgcatca
     8761 atcgcgaata caccctggtg ggagctgctc aaggatcaag agctccaaaa gctgattcgt
     8821 acggcactgg aagaaaataa ggacctccag cgcgcggcgg cggcggtgga ggagttccaa
     8881 gcccgcctgt ttatcgctaa aacagatttt gccccgcaga tgaatgtcac gtcgaatgcg
     8941 ccgctgttcg gtcggaagac caatttcttg tttcccggct tccccaatcc cttcaactac
     9001 tacctccagg gaaatttgtc ctgggagatc gacatctggg gacgtattcg tcgatccaat
     9061 gaggccgcgc gtggcgatct gttgtcacgg gaggagagtc ggcgcgcgat tgtgttgcag
     9121 ttggtgagtg gtgtcgcgga ggcctacttt gagctgttgc agttcgatat gcaactcgac
     9181 attgcgcggc gtacgctcaa gtcctgggag gagtccgtca ggatcgctca ggcgcgattg
     9241 cggcagggaa tgatttccaa gctcgatgcc gatcaattcg aggccgaacg ggctaatgct
     9301 gcggctaagg cggcggagtt cgaacgtcag aaggtacaga aggaaaatca actcagtgtg
     9361 ctgctgggcc ggaatccagg ccggattgcc cgcgggcatt ccttgacgga gcaagtgatg
     9421 ccgccggacg tgcctcccgg ccttccttct gaattgttgc agcggcgtcc tgacattctg
     9481 caggcggagc aggatcttgc cgccgccacg gctcgcatcg ggatggcaaa agctgatcgc
     9541 tttccgaagc tcagtatcac cggcatcctg ggtgtggcga gtccgcattt gtcccgttta
     9601 gtggccaatg aaaccgcgtt cggtgtcgcg gggccaggct tcgccggacc attgttgaat
     9661 gctcagatcc tcggtttcca acagaaagct gccgaggcgc aagcgcgaca agccgtggct
     9721 caatatgagc agtcggtgtt ggtcgcgttc aaggaggtgg aggattcgct ggtcgcagtc
     9781 agcacggttc gtgaacagcg cacggcacaa ttgcagcagg tcgacgcgtt gcggtctgcg
     9841 ctgagtcttg ccaatcttcg ttacaagggt ggcctggcaa attacctgga tgtgttgatt
     9901 gcgcagcgca atctttttga ggctgagtta gcgctgatgg gaacccatcg gctacatctc
     9961 gtctccattg tgcaattata taaggcgctt ggcggtggct ggaccccgga agggcagccg
    10021 ccggttcagg ccctgccgct ggtctctaca caggaaacgc gatagtgacg ttgcctcttg
    10081 aggcgtaggt ctagtctgtt gctatgccct gatacttgtt cgctcgcctg ctttctcgtc
    10141 gatctcccat aagctaagcc gcagcccctt cacctcgctt caacctgagc gctcccggat
    10201 ttttcgggtt gtgcttccgg tacctgaaag acattcacgt tcgacgatcg gctgccgatg
    10261 tcagggtgag gatcatggcg aggcttccac ctaaggaggc catgagcagg atggtgaggc
    10321 cgctgtgtat ccatgctgag ctggtttgga gtcccagtac gatattcgta aggttgaagg
    10381 tccagagggt tgccgccacc catgccaagt gttccgctcg gttggcggag aggcgagtgc
    10441 ccaccagggt gaagccgagc actcctgaca acagattaca gagatatgcg gttgaagaat
    10501 tgttctcaag ggttattccg gctgcagcca tgcacagccc cgcggcaaat gtcagcccat
    10561 aaacgatcgc ggcatcgcgt accacagttt tggcggtgga ggtcactgca gcctcccctg
    10621 ttgatgatcg tctcctggcc gactgacttc taattgcttc atgatcaaaa agagaagagt
    10681 ttgagagatg ccggtagaac gagtgctcgg tagtgacgca catttcatgc tctcggctgg
    10741 gcgacgtttc acctgtgcct ccttttgaat ctcggcggaa cgtgcaatcg gaaacgaatg
    10801 cagaggagga acagaccaac agtaaccggt ctcctgttcc ttgcgggtgc gaggttgaat
    10861 gacggggcga gccgacatgc tgtcatagcc tttttttctg ggtcgaactg agaggcgggg
    10921 gagaagaaag tgattttcgg ttgcgcagcc aacggctcaa acgtcgagac atcgcataca
    10981 ccaggttatc aattgcgagt acgaccgcgc cgattaaaaa cagccccaga atgagagcga
    11041 gctctacatc aatggacatt gtttattcca ccggtctaga catgagtgag tagcgaagag
    11101 ggatgctcgt tatctgcata tatttcactg caagacaatc gtgaccagga tgcctccggc
    11161 cgtgatttcc ctgacatcct ggtctttgca ttgagactag gcagccagct gtgccatgcc
    11221 tcctgatccg aagtgggcat gctcactgtt tgggatcgtg ggagcggatt tggaggagtg
    11281 cagtgcggcg ccctgagtct gtcggttgtg ctccaacacc ttctcggcca catttccgca
    11341 gttcatgcat cgtttggctt ggagccacat gggccccgac gagtcctcca tatccagcag
    11401 gaaatcttca accatgcatc cgttgcagcg agaacacgtc atagtggcct ccttcattgt
    11461 tcagtgccta tgtgtaagcg tagaaccgga tggtcatctc cgacagacct gaatgaagta
    11521 cctccttggt ggtaccgcca ggtgggatgt ttttactggt ggccaaatat tgataggtgc
    11581 atcgagtctt cgctcgccgg gaggcgcgga gatggaagcc actcggcatc atgcccacct
    11641 ttttcaagag aactattcct gccgtctgta cgtggggcta ttccgcggct gtaatcgtga
    11701 gttcctggtg tgagtcttga aattccagtt cgagggggag gttttcttcg aggaaaccat
    11761 tcaatcgagc ccgttcatca gcatccatca tgaggagttc cactccgaat cggtttcctt
    11821 gtgcccaggt gactctcgcc aaacctaccg aaaccggtcg gcgatcttca ggaacctgca
    11881 gtttgagcgc aaggtagtct ccgtcgatga ggctgtgtga ggttgggtgc ggcgccccga
    11941 cgagaccgga atcgatgatc cgcccttttt ctgcgcagcc catggccgca gcagaaagga
    12001 tgcagtgaac gacgaatctt cgatgcgatg aacgtgtcat gcgaacctcc cgcgtgtggc
    12061 gaacgagcac gacgaaaccc cggtgatgaa cctatctcgg ctatgacgga tcagatagtc
    12121 tgcgaagagg gtacttctaa cgaggggcac gtcaggggcg cggagcctta aggatctgtg
    12181 gcgagcgtcc tagggttgag gaaaataggc cattgaagag tacgaattaa cgggcagtag
    12241 gtgtgtctgt ggaggtgagc atcgaagtgc ttggtggggg ttgttgttcg gtcaggtagc
    12301 gttctaaggc tgtgtcgtcc tgcttttcaa ggcgtcgtgt cgcgggaggt tggtagattt
    12361 ggtgaataca acagggctca ttcaaaatgt aagacgcttt tcaatccctc gtttttcggt
    12421 cgataatggg gcgtgtggat gaggcgtgta tggacggagc tgtccagcac ttcggtattc
    12481 gttcccgtct gcatgactgg tcgatagaat cgtgaatgaa accacttccg gatccttcca
    12541 aaacactcgc taaacgaatc gtgaatcgga cttagcgcat ttggctgcag cggaaccaaa
    12601 cgggtttcaa tagccaatcc cttttccatt gagtgccgct gtagccaggc caaggcgaca
    12661 tctgccaagt cccgttccgg gtaggccccg ccgagatcac cgtgtgcccc tgcgaaccag
    12721 cgttgttcaa tggtctgccc cgggcaggcc ggtgaggtcc acagcgtcgc gttgtgatct
    12781 gcgcggtgtt cgtcgatggc gagtgcatga taggcctgtt ttacgatcga actcagttcg
    12841 gtatcgtgaa aattatagcg gtggtcgttg agccatttga gggcgttggt cggaattcca
    12901 agtgggccga cggtttccca caacccgagt agcgtgatcg gaacttccag tgttcctcgc
    12961 cgtctcgcgg aagtaggcat tcctgcaagg atatcgccgt gacccttacg atagaggcga
    13021 taggcatcgt cgagggcctg gttggagggc tcaagaatca tctcatgtag gctttgtcga
    13081 agagcagtgg gtgttgtagt cgaaggtgac ggatgggaga cgacctcacg cacgcgcttg
    13141 acaagatcgg tatgtagaag cccggcggga atgaggccgg cggtggtgag caggccgacg
    13201 agagaacgtg cggtatacgc acctcggctg aagccataga caaatagctc atcaccgggc
    13261 tcgtaggtgg cggcgaggta ggcgtaagcg tgaaggatgg tctgatccac tccataccca
    13321 aatgagccag gccggaaccg ataaaaccag ggaatgtcgg caccggagtc ataccatttc
    13381 tgttgcgcac gtccgtcctc cgtccgcggg aggattgaat ggtagagccg cagtacattc
    13441 gtggccggag atagatgacg gtgggagtta tgtagggttt gtgtgttggg agtgctcgat
    13501 gagggcaact gcctgctagg gtctggaggt aaatcccaca tgccatcgaa gcacagaatc
    13561 aaccgtttgg acacgcgact tctttctcct aggcgcacga gaaacggtta gagctgttct
    13621 tgtgatccgc ttgcttcgat aggaagagag caggcccaag cagcgctgta gccgcattgc
    13681 tcgcccatgg tagagcaaac ttaatgccat tattgtttgg tgccggggtg agttggaatt
    13741 gtgtcgatgc aggtgatcga tagccatgtg aacaactcaa tcactgcctt ttcaggctga
    13801 atcctgagga aagagaggaa ataaggggct atctcacgat agacctgtat caaatgggat
    13861 acggattcgt atccgaaagc atacgtgcat atgtgacatg tgtgcgtgag tcgcgagtcg
    13921 aggtggagtc gaggctgtgg ctcgaagcag atatgtcagg agagtttatc ggtgagtagc
    13981 cttgatgaca aactcgcgat cttgttcaat ctgagtggcc aattggtgca gggcttgggc
    14041 ggtggcctcg gcgatcggct cttgatccga tgcggtaacc catctcactg aggagccaag
    14101 ggcttcacct tcggctgcgt atgtcttgag gggggcttgt ccggaaaagg caaggtcgga
    14161 ttccaaatgg acccggtaga ggtagcggtc aggcgcgcga agcatgagcc aggctttcac
    14221 aaccaggcgc atgtcaccgg cttctgttcc gatagacgtg aaggtctggc gctgagtgaa
    14281 atattcgaca atcgtttgtt gtaagtcttt cgacggccat tcgaggaggg gaatacctgg
    14341 catgtgatcc gccccttcta tggcgagaaa cggtacctca agctgcaccg tcgcggggat
    14401 cggggccgca gccctgtcgc tcgatggtga ggtgacgtga atatgatgca cacacccggc
    14461 cccgaccaga taggtgccaa gcaacatgat tgtgagtcgg tacttgagac cccgtgtggg
    14521 gcgacgcatt ctgaactcca tggttagtgg gaggaagggg gcggggtgag gatgtctaag
    14581 tcacgaatgg ctcgcgccgc ctgctcccga taggtccgct ctgtcgtgcc ggtgtaggta
    14641 tcaatgacac gttggtaggt tgctcgggat tgcgcatatt gcttcttgat cgagagatat
    14701 tgtcccaagg ccagccagtt ctgggcggcc gtctcattgg cggtgcgaag gtcggtccat
    14761 tcgcgatcga cctggttggt tcctggacga ttgacgcgcc gagagatgat gtcgcctaac
    14821 tgggatgaga ggtgttcgat cgcttcattc tcccggacgg ccgctgccac gcgatcatgt
    14881 gtcaagtgat cgtagaaggc ttcgacatga tccttaacgg tgtcggcccg tttctcgtgg
    14941 atgtcgtggg agcagtgcgc gagggtgagg caggccgtga gcaataccag cccgtgccac
    15001 atcgaagctg tacgtgagcg aatcgccatc gtcgcctcct cggtaaatcg gacgtttgag
    15061 gtccgatgtt tttcgtctcg tgtcgcgatc ctaccgtagc tgggtgtgga aaggcgagcc
    15121 tagggacggc gctaggaggg ggccttgaag aacagcacag agagcggggg cagcgtcatg
    15181 gccaacgagt gcgttcgatc gtgggccgga atgggctcag ccgagaggcc gccgagattt
    15241 ccaaggccgg tgccgccata catggacgcg tcgctgttca acagttcttt ccaatagccg
    15301 ccttgtggga caccgacgcg atactgcagc cgtgggactg gcgtaaaatt acagacgacg
    15361 gcgatgtgtt gctgtggtgt tcggccgtgt cgcaagagac tgatgacccc ggcatccacg
    15421 tcctggcagt cgatccactc gaaccctcgc gggtcgaaat cgaattcatg cagcgccggc
    15481 tctgtccggt agaggtgatt caggtcggcg acccaccgtt gcagcccttg atggggctgg
    15541 tactggagca ggttccaatc gatgctgtcg tcgtgggccc attcgcgcca ctggccgatt
    15601 tcaccaccca tgaaaatcag tttttttgca gcctgggcgt acatatagcc gaacagcgca
    15661 cggagattgg cgaatttctg ccaatcgtcg ccgggcattt tgcccaggag cgagcctttg
    15721 ccatggacga cttcgtcatg ggacagggga agtaaaaaat tctcttggaa cgcgtagagc
    15781 atgcggaagg tgaggttccg gtgatgatgt tttcggtaca cggggtccag cgccatgtac
    15841 tccagcgtgt catgcatcca tcccatgtcc catttcatgc cgaagcccaa tccgcccgca
    15901 tacgtcgg
//
LOCUS       DAOQRM010000050        15666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2851_length_15666_cov_18.333163, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000050 DAOQRM010000000
VERSION     DAOQRM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15666
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2851_length_15666_cov_18.333163"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2267
                     /locus_tag="PKK81_05980"
     CDS             <1..2267
                     /locus_tag="PKK81_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV32454.1"
                     /translation="IFQKEYFSPLIETQMNRCVQCLRCVRYCDEIMDVKALAPVGRGT
                     MTEIKHFGPHELDCEFCGGCIQICPVGAITSRLSMYEYRPWMLKRADTICTFCGDGCR
                     ITVQTKDNELIEVNSAHGAGRNNGDLCARGFFGFHASSHADRLTHPLIRREGKLVETT
                     WEDALEYVAEQALRLKLAQGADAFGGLISSRCTNEDLYVFQKFMRQVIGTNRIDSSAR
                     YGHLNGVQALRRVQGTHRWTIAFEDIVAANALLLVGTNITETSPITGLKVKEAVKKRQ
                     ASLVTVETLQPAIDTLSNITNLALQHFPTHPAQFGNTVLGVTKAVLEANLVDAAVAQQ
                     APAFVQRLTSALQGTSWEALEAATGQSRAQWAEAATVLAKANRLVILVGNGVLRHPGG
                     DATVTNLLDLLILLGKLDKPGCGLGPLAEENNDQGAVEMGAVAEFFPGPAPLDDQPVR
                     DRIAAMWREDLPRTPGASLTEMLAAANKGTLKAMFVVGENPVGTLPAAAQAKEALGKL
                     DLLVCQELFLTETAAMAHVVLPACSYMEKDGTFTNSEGHVQGVRQAINPVGDSRPDWE
                     MLSALSVLMGSPLEYGDAREILKEIRTVIPGYGLLGPTPTPPKVDPMVLNRYLTEGFA
                     VDAATRYAVSQPRQTNDGPFTLMFVQTLFHSGKLSTRAKGLLQLQQEGFLSINPADAA
                     QLGLADGGQVKVSNSRGAITTTVKIRERVPAGLLWFPEHFDGEAKQLAEWTIDPRTQI
                     PYFKLAHVSLAKVS"
     gene            2292..3359
                     /gene="nuoH"
                     /locus_tag="PKK81_05985"
     CDS             2292..3359
                     /gene="nuoH"
                     /locus_tag="PKK81_05985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNV32455.1"
                     /translation="MEIGLRLAVSLAQIAAVMGVVMLTVMVLTLAERKVLGWMQDRMG
                     PMEVGPYGVLQPIADGLKLFFKEDIIPAGANKFLFTLAPILALVPAMIGFAVIPFGPS
                     LTIELFGMQIKPFVISDINIGILYILAFASIGAYGIILGGWSSNSKYSLLGGLRSAAQ
                     VISYELCVGLAIVGVILLSGSLSLVKITEAQAGGFWNWFVIAMPFPQIFAFVVYVISA
                     VAETNRVPFDLPEAESELVAGFFTEYSGMRFAFFFIAEYANMILVSCVAAALFLGGWN
                     APYPGTILGYIGLDSLAWIENVVWFAAKVYFFLFLFFWLRATLPRLRYDQLMRFGWKV
                     MLPIALGNIVLTAIAAYFFPR"
     gene            3369..3944
                     /gene="nuoI"
                     /locus_tag="PKK81_05990"
     CDS             3369..3944
                     /gene="nuoI"
                     /locus_tag="PKK81_05990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNV32456.1"
                     /translation="MNVAVTTQPKRKPSFSDWLKTLTFYELLVGMKATLTHLLNYKPI
                     TLQYPHEKRLLPDNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSGEVPGEPT
                     KRYSKEYYMDMTRCLFCGLCVDACPVDALGMTREFEWAVYDKRQLHLNKQQLLAIGDR
                     SFPVREKRLELQHPNVAFFNVTFTHLPQKEN"
     gene            4075..4587
                     /locus_tag="PKK81_05995"
     CDS             4075..4587
                     /locus_tag="PKK81_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNV32457.1"
                     /translation="MDHIFFFYFAAVIAATSLLVVALRNPVYSALSLLIMFFHVAGLY
                     ITLHAEFLAAVQIVVYAGAILVLYLFVVMLLSIKSEERYHNQLPVAGLLGVVLCTEVI
                     LLFIKSQTRGMAPAAAADPVAGPGNTEMIGEALYSTYLFPFEVASLILLVAMIGAIIL
                     AKKDINEPVQ"
     gene            4602..4907
                     /gene="nuoK"
                     /locus_tag="PKK81_06000"
     CDS             4602..4907
                     /gene="nuoK"
                     /locus_tag="PKK81_06000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNV32458.1"
                     /translation="MAVPLSYYLILSGFVFLTGVVGVLIRRNIIVILLSVELMLNATN
                     INFVAFSEYFHNVAGQVFVFFALTVAAAEVAVGLAIIIALHRSNSSIYVDDLNLLKR"
     gene            4924..6822
                     /gene="nuoL"
                     /locus_tag="PKK81_06005"
     CDS             4924..6822
                     /gene="nuoL"
                     /locus_tag="PKK81_06005"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNV32459.1"
                     /translation="MLYALIPFLPLFAFLIVGIGEQWIKDRAHLVAVPAMVGSFLLSL
                     LALHDVATGQAINVPLYTWLTSGNLDIHIGISIDRLTAVMLILVTTVSTLVHIYTIGY
                     MHGEPGYARFFAYIALFTFSMLMLVMADNLLQLFVFWEAVGLCSYLLIGHWYERASAC
                     AAATKAFLVNRVGDFGFILGLFLVWYSFGSLDYATVFAHAQDLASKTTNLLGPFGGTW
                     DVSVMTMICLLLFTGAVGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVAR
                     LSPLYNLSPTAMTVVALVGGLTMMLGATIALTQTDIKRVVAYSTMSQLGYMVMACGLG
                     AYSAGMYHLLTHGAFKALLFLGCGSVIIALHHEQDMRHMGGLKDKLPVTYWTFLVGSL
                     ALAGFPLTAGFFSKDDLLVSSWSSGPLGQVLTICGLLTAGLTAFYSFRLVFVTFWGKS
                     RVDPHHAGHVHEPSTTMTAPLLVLAVLSIVAGYLGIPAFLEPVFHGDGAAAHHEGSAA
                     YGIMAVATLMGLTGIGAAYYLYVLNPGLPDRLAQQWRAAYELSLHKWYIDEAYDRSLV
                     RPTLSAAQGLWKHVDVAIIDGAVNGVARAIAWGGWLIRLTQSGQTQHYALGMTLGAVV
                     ILTVYLLF"
     gene            6850..8418
                     /locus_tag="PKK81_06010"
     CDS             6850..8418
                     /locus_tag="PKK81_06010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNV32460.1"
                     /translation="MTSFPWLSLIVFLPLAGALLCLLVKAESARWLALGVTVADFALS
                     LPLWWLFDSSTAGMQFVERASWISSPPVQYSLGLDGISFPLVLMTTFLMPFCVTVSWT
                     AIEKRVQLFMSMLLVMETAMLGVFVALDFVLFYVFWEAMLIPMYLLIGVWGGPNRLYA
                     AIKFFLYTLAGSVLLLVAILALYFQGGHTFDILALSRGTYSASLQMWLFLAFFAAFAV
                     KVPMFPFHTWLPDAHVEAPTAGSVILASVLLKMGTYGFLRFTLPMLPDATVSFTKPMI
                     ALSIIGIIYGAYMALAQTDIKKLIAYSSVSHMGFVTLGIFVLNIQGIEGAVMQMVNHG
                     ITTGGLFLCVGIIYERTHSRQIQDNTGLAGPMPRYALFLMIFALSSLGLPGTNSFVGE
                     FLVLAGTFVWSQFATALAALGVILAAAYILWLMQRVVFGTPSAAHRAHLLDLNARETA
                     TLVPLIVLVFALGIFPNPLLSRMHASVTQLLGGPKNPTAAVEQQQAPVTAGKPTAAIA
                     AILSPSSSEQAAAR"
     gene            8415..9890
                     /locus_tag="PKK81_06015"
     CDS             8415..9890
                     /locus_tag="PKK81_06015"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNV32461.1"
                     /translation="MTFPLQDLLTILPELIVIGTACLILALDPILEASKKDVLAWLTL
                     GALAMCIGLTSSQMTGRVSAFSGMVVIDAYAAFWKLLLYIVTGLTVLLSLAYLKAERL
                     SIGEYYGFILLALAGMMVMVSGADLLTIYLGTELMSLSLYVMAGLKRTEARSLEASAK
                     YFVLGAFSSGILLYGISLLFGLAGSTGLSAIAAAITTHGTGDPLLSMALVLLAVGFGF
                     KMAVVPFHMWTPDVYQGAPTSVTAFMAVASKAASFGAFLRVFVEGLGAASADWSVLFT
                     VICLATLALGNLVAIVQTNIKRMLAYSSIAHAGYALIGVVVAGGHSGEGASTGFASVL
                     LYIAIYSFMTLGAFALIGMLRKEGQESEQIEDYAGLAKREPLAAFFMLVFMVSLAGIP
                     PTAGFIGKFYIFMAAVNGGMTWLAIVAVIFAAISAFYYLRVVMVMYMREAEGSVATHS
                     RLETSPALSLVLACALAGVVLLGLFPNGLWSLASQAAPLLK"
     gene            10020..10880
                     /locus_tag="PKK81_06020"
     CDS             10020..10880
                     /locus_tag="PKK81_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNV32462.1"
                     /translation="MTELSFLSNVFDTFRRRGCLSLAASLAFFSLLSLFPMVFLLLYG
                     ISFIVSQDVIGYQFLLGFLKGFLPSMGERLARDIRRVAEQEEVRWAVFLAFGWFSALV
                     FYELDYAMNTVFGTAAKRHPLISTLVAVALIWMLGFLTLISFVATQAIELLTAYAPRF
                     LGMNLVAIAAHDFLIAYSLPFLLAFASVTCLYRFLPHQRPSWREATIGGAVFSLLWVS
                     AKALFVTYLEDAAVYTQLYGSLLEVVLLMLWVYYSSALVLLGAVVTHECQCRRPSYSK
                     EASPDRAEVV"
     gene            complement(10937..12109)
                     /locus_tag="PKK81_06025"
     CDS             complement(10937..12109)
                     /locus_tag="PKK81_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNV32463.1"
                     /translation="MQGPDWKIGRMFGIPIHVHASWLFVFFFVTWSLATGYLPDVLPG
                     LTEPRYWAMGGVAAILLFASVLLHELGHSLVALRYRIPISQITLFIFGGVAQMRKEPP
                     HPRAEFLIAIAGPIVSFLLAGLCIGMVALLEFLPTGSSVYGLVALGTLLGMVNTQLGL
                     FNLLPGFPLDGGRALRAGLWAWSKDYYRATSQAALVGLLFGVSFGLFGAFLLVGALSG
                     TVSGALASSGGWIVLLGAFLFAAARGSRKQAAIRGSLASVPVRELMVSNVVALSPDTT
                     LEEAVNQYFLPYGYGGFPVVQDGRLVGVVGVRDVQTVSNGLWAFRRVADIMQTSSDDM
                     VVSPDLSAMQALEQMLALGAERLVVVQDGQLLGLVTRASIGHFIEQRHPSGKGSSS"
     gene            12227..12904
                     /locus_tag="PKK81_06030"
     CDS             12227..12904
                     /locus_tag="PKK81_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV32464.1"
                     /translation="MALALQAILAEAQVIKAGPASCPAVALTYDLCPVRTASGFDREL
                     IDFLIEHKVPATFFMSGRWMAKHDAEVKKLLAVPFFEIGTHGEVHAHLPMHEVEEQQQ
                     EIMGPVRLLKTKYGRPATLFRPPYGEYNDQTVDAVKTLGLKFILWNIESGDPDPTLTT
                     EAIITRIQRRMKPGSVIVMHANGKGKHTREVTETLAATLLPAKGLTPMTVSDLLRCNQ
                     STATPAP"
     gene            12913..13362
                     /locus_tag="PKK81_06035"
     CDS             12913..13362
                     /locus_tag="PKK81_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV32465.1"
                     /translation="MRAEDRDPVIEILTGSDPWKRLGFTAADWNRIFAPLPTGRDTFV
                     LEIEGNVLGIAIVRRKFLFGDYLELLGISPSAVGQGLGSRLLTHVESLTFARAVNMFA
                     CVSDFNEGARAFYRKQGYKEIGPMPNFLIPGYAEILLRKTTGPARKS"
     gene            13428..13826
                     /locus_tag="PKK81_06040"
     CDS             13428..13826
                     /locus_tag="PKK81_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV32466.1"
                     /translation="MKVTKLLHTRMRVSDMDQTIRFYQHVLGLEVLERKVSPRGSHLA
                     FLKVPNSEELIELCSFPASGPVTVQEDLVHLAFQVENLDDTIRALESKQIRITDGPTK
                     TSSGSRFIFIDAPDGYEVELIERPPGTALV"
     gene            13987..14856
                     /gene="dapF"
                     /locus_tag="PKK81_06045"
     CDS             13987..14856
                     /gene="dapF"
                     /locus_tag="PKK81_06045"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNV32467.1"
                     /translation="MKNGFFRGHGLGNDYVVMDPTELTFKLTPGKIRGICDRHWGLGS
                     DGILALVPSKKADFGLRIYNPDGSEAEKSGNGLRIFARYLHATGKTKKRAFTVETKGG
                     LVSIALHLDRHGDAAAATVEMGRATFQPSALPCTLTVDELIQQPIEAAGRSLQFTGVS
                     VGNPHCVIFKQPGEAWTREDLLTLGPALENHSLFPKRTNVQLAVPTGPKDIFILIWER
                     GAGETQASGSSSCAAASAAVRLGLVKGPVTVKMPGGTLNIEVAQDFSLTMKGPVAEVA
                     RGALSPSFVRGLK"
     gene            14936..>15666
                     /gene="uvrC"
                     /locus_tag="PKK81_06050"
     CDS             14936..>15666
                     /gene="uvrC"
                     /locus_tag="PKK81_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNV32468.1"
                     /translation="MTTDVLQSKLDHLPGQPGCYLFRNAKREILYVGKAAVLADRVRS
                     YFQRGSDQTPKTALLVNEIADLETIVTRSELEALILESNLIKRHRPRFNIVLRDDKQY
                     PYLRLPIKEHFPRLSIVRRVQKDGALYYGPYTPAGALRETLKVIRKAFPLATCEIEID
                     GRADRACIEFEIKRCMAPCTGNQSRDEYHQIVKQVRQFLEGRDRELLDSLREDMERAA
                     EREAFEEAARLRDRLFSVERTLEKQR"
BASE COUNT         2956 a         4969 c         4514 g         3227 t
ORIGIN      
        1 gcatcttcca gaaagagtac ttcagccccc tcatcgaaac gcagatgaac cgctgtgtgc
       61 agtgtttgcg ctgcgtccgg tattgcgatg agatcatgga cgtgaaagcg ctggctccgg
      121 tggggcgcgg caccatgacc gagatcaaac acttcggccc gcatgaactg gactgcgaat
      181 tctgcggagg ctgcatccag atctgccccg tcggcgccat cacgagccgg ctctccatgt
      241 atgagtatcg cccctggatg ctcaagcggg ccgacacgat ctgcaccttc tgcggcgacg
      301 gctgccggat caccgtgcag accaaggaca atgaactgat cgaggtgaac tccgcacacg
      361 gagccggacg gaataacggc gacctctgcg cccggggctt cttcggcttc catgccagca
      421 gccacgccga ccgccttaca catccgttga tccgtcgcga aggcaagttg gtcgagacca
      481 cctgggaaga cgcgctggaa tatgttgccg aacaggcgct ccgcctgaag ctggcgcagg
      541 gcgcggatgc gttcggtggg cttatcagct cccgttgcac gaacgaagac ctctatgtgt
      601 tccaaaagtt catgcgccag gtgatcggca ccaaccggat cgacagcagc gcgcggtatg
      661 gccacctcaa cggagtgcag gcgctccgac gcgtgcaggg tacccatcgc tggaccatcg
      721 cctttgaaga catcgtcgcc gcgaatgcat tgctcctggt cggtacgaac atcaccgaga
      781 ccagcccgat caccgggctc aaggtcaaag aagccgtcaa gaaacggcag gccagtctcg
      841 tgaccgtgga aacgctccaa ccggccatcg atactctgag caacatcacg aacctggccc
      901 tgcagcattt cccgacgcat cccgcgcaat tcggcaatac cgtgttaggc gtcaccaagg
      961 ccgtcctcga agccaacctg gtcgatgccg ccgtcgcgca gcaggcgccg gccttcgtgc
     1021 agcgtctgac ttccgctctg cagggcacct cgtgggaggc cctggaggcg gcgaccggcc
     1081 agagccgcgc ccaatgggca gaggccgcca cagtactggc gaaagccaac cggctggtca
     1141 tcctggtcgg caacggtgtg ctgcgccatc ctggcggaga cgcgacggtc acgaatcttc
     1201 tggacctgct gattctgctt ggaaaactcg acaagccggg ctgcggactc gggccgctcg
     1261 ccgaagaaaa taacgatcaa ggtgccgtcg aaatgggcgc cgtcgccgaa ttctttcccg
     1321 ggcccgctcc gctcgacgac cagcccgtcc gcgatcgcat cgcagccatg tggcgagaag
     1381 acttaccgcg cactcccggc gcctcgctga ccgagatgct tgccgccgca aacaagggca
     1441 ctctcaaggc catgttcgtg gtcggggaaa acccggtcgg caccctgccc gccgcggccc
     1501 aggccaaaga agccctgggg aagttggacc tgctggtctg tcaggaattg ttcctcaccg
     1561 agacggcggc gatggcccat gtggtgctcc ccgcctgctc ctatatggaa aaggacggca
     1621 ccttcacgaa ttccgaaggc catgttcagg gggttcggca agcgatcaat cccgtgggcg
     1681 acagccgccc cgactgggaa atgttatcgg cgctctccgt gctgatgggc tcgccgcttg
     1741 aatatggcga cgcgcgggaa atcctgaaag agattcggac ggtcatcccc ggttatggac
     1801 tgttaggacc gactcctacg ccacccaaag tcgacccaat ggtgttgaac cgatatctga
     1861 cggaagggtt tgcagtcgac gcggccacac gctatgccgt gagccagcct cggcagacta
     1921 acgacggccc gttcacgctg atgttcgtgc aaacgctgtt tcactccggg aagctgtcca
     1981 cacgcgccaa ggggctcctc caattgcagc aggaagggtt cctctccatc aatccggcgg
     2041 atgcagctca gctcggcctg gcggacggcg ggcaggtgaa ggtctcgaac tcacgcggcg
     2101 ccatcaccac aacggtgaag attcgcgaac gggtgccggc cggcctgctg tggtttccgg
     2161 aacatttcga cggcgaggcc aaacagctcg ctgaatggac gattgatccc cggactcaaa
     2221 tcccctattt taagctcgcg cacgtgtctc tcgcgaaggt ctcgtaggac gggacgagga
     2281 gtattgttgt catggaaatc ggattacgac tggcggtgtc gttagctcaa atcgccgcgg
     2341 taatgggagt ggtgatgctg accgtcatgg tcctcaccct cgcggaacgg aaagttctgg
     2401 gctggatgca ggatcgtatg ggcccgatgg aagtcggccc ttacggggtg ctgcagccga
     2461 tcgccgacgg actcaaactc ttcttcaaag aagacatcat cccggcggga gccaataagt
     2521 tcctgtttac ccttgcaccg atcctggccc tggtgccggc catgatcgga ttcgccgtca
     2581 ttccctttgg cccgagcctg accatcgaat tgttcgggat gcagatcaag ccgttcgtga
     2641 tcagcgacat caacatcggc atcctgtaca tcctggcctt tgcctcgatc ggcgcttacg
     2701 gaatcattct gggtggctgg tcctcgaaca gtaaatactc cctcctcggc ggactccgct
     2761 ccgccgcaca ggtgatcagc tacgaattgt gcgttggcct ggcgatcgtc ggagtcattc
     2821 tcctgtccgg ctcgctcagc ctggtgaaga tcacggaagc gcaggccggc gggttctgga
     2881 actggttcgt catcgccatg cccttcccgc agattttcgc cttcgtggtc tacgtcattt
     2941 cagcggtcgc tgaaaccaac cgggtgccgt tcgacctgcc cgaagctgaa agcgaactcg
     3001 tcgcgggctt cttcaccgaa tacagcggca tgcgcttcgc cttcttcttc atcgcggaat
     3061 acgccaacat gattctggtg tcctgcgtcg cggcggccct gttcctcggc ggatggaacg
     3121 cgccctaccc gggcaccatc ctggggtaca tcggcctgga cagcctcgcc tggattgaaa
     3181 acgtcgtctg gtttgccgcg aaggtctact tcttcctgtt cctgttcttc tggttgcggg
     3241 cgacgctgcc ccgcctgcgg tatgaccaat tgatgcggtt cggctggaaa gtgatgctcc
     3301 cgatcgcact gggcaacatc gtcctgacgg ccattgccgc ctatttcttc ccgcggtaac
     3361 ggagcgaaat gaacgtcgcc gtcacaacgc aacccaagcg gaaaccgtcg ttcagcgatt
     3421 ggctgaagac gctgaccttc tacgaactcc tggtgggcat gaaggcgaca ctgacacacc
     3481 tgctcaacta caagccgatt acgctgcaat acccgcatga gaagcgcctg ctgcccgaca
     3541 actaccgggg catgctggcc ttgctgcggt atgacgacgg gacggaaaaa tgcgtgggct
     3601 gcgatctgtg cgaagcggcc tgtccctcgc gggtcatccg ggtcgtcagc ggagaagtcc
     3661 ccggggaacc gaccaaacgg tattcgaagg aatactacat ggatatgacg cgctgtctgt
     3721 tctgcggtct ctgcgtcgac gcctgtcccg tggatgccct gggcatgact cgcgagttcg
     3781 aatgggccgt ctacgataag cgccagctgc acctcaacaa gcaacaactg ctcgccatcg
     3841 gcgaccgttc atttcccgtc cgggaaaaac gcctcgagct gcagcatccg aacgtggcgt
     3901 ttttcaacgt caccttcacg catctcccgc aaaaggagaa ctaggccacc cgcctcgtcc
     3961 tccgtgctgg ccccccacac cgagacgacc gaggcggaac aggctgccac cgggcagggc
     4021 gcaagggcaa cagggggcag gaccttcatc tgattcggaa gagacgacgg acgcatggac
     4081 cacattttct tcttctattt cgccgcagtg atcgcagcca cctccctgct ggtagtcgcg
     4141 ttgcgaaacc cggtctatag cgcgttgtcc ctgctgatca tgttttttca cgtggcgggg
     4201 ctctatatca ccctgcatgc ggagtttctg gccgcggtgc agatcgtcgt gtatgccggc
     4261 gcaatcctgg tgctctacct cttcgtagtc atgctgctga gcatcaagtc ggaggagcgc
     4321 taccacaacc agctcccggt ggcgggattg ctaggggtgg tgctgtgtac cgaagtgatc
     4381 ctgctgttca tcaagtcgca gaccaggggg atggctccgg cagctgcagc cgatcccgtc
     4441 gcaggaccag gcaatacgga aatgatcggg gaagccttgt actcgaccta tttgttcccg
     4501 ttcgaggtcg cgtcgttgat cctgctggtg gccatgatcg gggccatcat cctggcgaag
     4561 aaagacatca atgaacccgt gcagtaacaa ccggtaatcc catggctgta cccttatcct
     4621 actatctgat cctgagcggg ttcgtgtttc tcacgggtgt ggtgggcgtg ttgatccggc
     4681 gcaacatcat cgtgatcctg ctgtcggttg aattgatgct gaatgccacg aacatcaact
     4741 tcgtggcctt ttccgaatac tttcacaatg tggcggggca ggtgttcgtc tttttcgcct
     4801 tgaccgtcgc ggcggcggaa gtggccgtgg gcctggccat catcatcgcc ctgcaccggt
     4861 cgaattcatc catttacgtc gacgacctga acctattgaa acgttagacc gactacgccg
     4921 accatgctgt atgcgttgat tccatttctg ccgttgttcg cctttctgat cgtcggtatc
     4981 ggcgaacagt ggatcaagga ccgtgcgcat ctggtggcgg ttccggccat ggtgggttcg
     5041 tttctgctct cactcctggc gttgcatgac gtggccacgg gccaggccat caacgtgccc
     5101 ctctacacct ggttgacctc cggcaacctg gacattcaca tcgggatttc catcgatcgc
     5161 ctcaccgccg tcatgttgat tctggtcacc accgtcagca cgctggtgca catctacacg
     5221 atcggctaca tgcatggcga accgggctac gcccgcttct ttgcctatat cgccctgttc
     5281 accttctcca tgctcatgct ggtgatggcg gacaatctgc tccagctgtt cgtcttctgg
     5341 gaagcggtgg gactctgctc ctacctgctg atcggccact ggtatgaacg ggcgagcgct
     5401 tgtgccgccg ccacgaaggc cttcctggtc aatcgcgtgg gagacttcgg cttcatcctc
     5461 ggcctgttcc tcgtctggta ctccttcggc tcgctcgatt acgccaccgt gttcgcgcac
     5521 gcgcaggacc tggcttccaa gaccacgaat ctcctcggcc ccttcggcgg cacctgggat
     5581 gtctcggtca tgaccatgat ttgcctcctg ctctttaccg gagcggtggg taaatccgcg
     5641 caggtcccgc tgcacgtctg gctccccgat gcgatggaag gtccgacgcc gatctcggcc
     5701 ttgatccacg ccgccaccat ggtcaccgcc ggtgtcttca tggtcgcgcg cctctcgccg
     5761 ctgtataacc tgtctcccac ggccatgacc gtcgtggcgc tggtcggcgg gctgaccatg
     5821 atgctgggcg ccaccatcgc cctgacccag accgacatca aacgcgtggt ggcctattcg
     5881 acgatgagtc agctgggcta catggtgatg gcctgcggct tgggcgccta cagcgccggc
     5941 atgtaccacc tgctcaccca cggcgccttc aaggcgctcc tgttcctcgg ttgcgggtcg
     6001 gtcatcatcg ccctgcacca tgaacaggac atgcgccaca tgggcggttt gaaagataaa
     6061 ctcccggtca cctattggac attcctggtg ggctcacttg cgctggcagg attcccgctc
     6121 accgccgggt tcttcagcaa ggacgacctg ctggtctctt cctggtcatc cggtcccttg
     6181 ggccaggtgc tgacgatctg tgggctgctg acggcaggat tgaccgcctt ctatagtttc
     6241 cgtctggtct ttgtcacctt ctgggggaaa tcccgcgtcg atccgcatca cgcaggccat
     6301 gtccacgagc cctcgaccac gatgaccgct ccccttctgg tactggctgt cctgagcatc
     6361 gttgccgggt atctgggcat tccagcgttc ctggaaccgg tcttccatgg ggacggggca
     6421 gccgcgcatc acgaaggatc agccgcctac gggatcatgg cagtcgccac gttgatgggg
     6481 ctcacgggaa tcggggcggc ctactatctg tatgtgctga atcccggctt gccggaccgg
     6541 ctcgcgcagc agtggcgcgc agcctatgag ttgtcgcttc acaagtggta tatcgatgaa
     6601 gcctacgacc gctcgctggt ccgcccgacc ctatcggcgg cgcaggggct ctggaaacat
     6661 gttgatgtcg cgattattga cggagcggtc aacggagtcg ctcgcgccat tgcctggggc
     6721 ggctggctca tccgcctgac acagagtgga caaacgcagc actatgcgct cggcatgacg
     6781 ctgggcgccg tggtgattct gacggtgtat ttgctgttct agtgcgacga acactataag
     6841 gacgcattcg tgacctcatt cccctggctg agtttgatcg tcttcctgcc gttggcagga
     6901 gccctgttgt gcctgctggt caaggcggag tccgcccgct ggctggcgct cggcgtgacc
     6961 gtcgccgact ttgcactctc cctgccgctc tggtggctct tcgattcctc aaccgccggg
     7021 atgcagttcg tggagcgggc ctcctggatc agctctccac cggtgcaata cagcctcggt
     7081 ctcgacggca tcagttttcc actggtgttg atgacgacgt ttctcatgcc cttttgcgtc
     7141 accgtgtcct ggacggcgat tgaaaaacgc gtccagctgt tcatgtcgat gctgctggtc
     7201 atggaaacgg ccatgctagg agtgttcgtg gccctggact tcgtgctgtt ttacgtgttc
     7261 tgggaagcca tgttgatccc gatgtatctg ctcatcggcg tctggggcgg tcccaaccgc
     7321 ctttatgcgg cgatcaagtt tttcctctac accctggccg gcagcgtgtt gctcctggtt
     7381 gcgatcctcg ccctgtactt ccagggcgga cataccttcg acatcctggc attgagccgc
     7441 ggcacctaca gcgcctcgct ccagatgtgg ttgttcctcg ccttcttcgc cgccttcgcg
     7501 gtgaaggtgc cgatgtttcc cttccatacc tggttgcctg atgcgcacgt ggaggcaccg
     7561 accgcaggca gcgtgattct cgcgagtgtc ctgctgaaga tgggcacgta cggcttcctt
     7621 cgcttcaccc tgccgatgtt gccggacgcc accgtgagct tcaccaagcc gatgatcgcg
     7681 ctctcgatca tcggcatcat ttacggcgcc tacatggccc tggcccagac ggacatcaag
     7741 aaactcatcg cctactccag cgtcagccac atgggcttcg tcaccctggg gatctttgtc
     7801 ctgaacattc agggcatcga aggggcggtc atgcaaatgg tgaatcacgg catcaccacc
     7861 gggggactct ttctgtgcgt cggaatcatc tacgaacgaa cccacagccg ccagattcag
     7921 gacaatacgg gcctggccgg gccgatgccg cggtatgcgc tcttcttgat gatttttgcc
     7981 ctgtcgtcct tggggcttcc gggcaccaac agtttcgtcg gcgaatttct cgtcctggcc
     8041 ggcacgtttg tctggagtca atttgccacg gccctggccg cactgggggt gatcctggcc
     8101 gcagcctaca tcctctggct gatgcagcgg gtggtcttcg ggaccccgtc tgccgcccat
     8161 cgcgcacatc ttctggacct gaacgcccgc gagacggcga cactggtccc gttgatcgta
     8221 ctcgtgttcg cgctgggaat ttttccaaac ccgttgttga gccgtatgca tgccagcgtg
     8281 acgcagctgc ttggcggtcc gaagaatcct acggcggcgg tcgagcagca gcaggctccg
     8341 gtcaccgcag gcaagccgac ggccgcgatc gcggcaatcc tttctccctc atcttctgaa
     8401 caggcagcgg cacgatgacc tttccactcc aggacctgct cacgatcctt ccggaactca
     8461 tcgtcatcgg gacggcctgt ctcattctgg cgctggatcc catccttgaa gcctccaaga
     8521 aagacgtcct cgcctggctg acattaggtg cgctggccat gtgcatcggc ctcacctcct
     8581 ctcagatgac gggtcgtgtg tcggcgttca gcggcatggt cgtgatcgat gcctatgcgg
     8641 cattctggaa attgttgctg tacatcgtca ccggactcac cgtgctgctc tccctggcgt
     8701 atctcaaagc cgaacgcctg agtatcgggg agtactacgg cttcatcctg ctcgcactgg
     8761 ccggaatgat ggtcatggtg tccggcgccg atctgttgac catctacctc ggaaccgagc
     8821 tgatgtcgct ctcgctctat gtgatggccg gactcaagcg gacagaagcg cgatcattgg
     8881 aggcgtcggc caaatatttc gtactgggcg ccttctcgtc cggaattttg ttgtacggca
     8941 tttcgctgtt gttcggcctg gccggcagca caggcctgtc ggccattgca gccgcgatca
     9001 ccacacatgg caccggagat ccgctcctct ccatggcgct ggtgctgctg gcggtcggat
     9061 tcggattcaa aatggccgtg gtgccctttc acatgtggac cccggacgtc tatcaggggg
     9121 ccccgacctc cgtgacggcg tttatggccg tcgcctcgaa agccgccagt ttcggcgcct
     9181 tcctccgggt gttcgtggag ggcctgggag cagccagcgc cgattggtcg gtcttgttta
     9241 ccgtgatctg cctggcgacc ctcgcgttgg gcaacctcgt ggcgatcgtc cagaccaaca
     9301 ttaaacgcat gctggcctat tccagcatcg cccatgccgg gtatgccctg atcggagtcg
     9361 tggtggcagg cggccacagc ggcgaaggag cctcgactgg tttcgcgagc gtgttgctgt
     9421 atatcgccat ctattcgttc atgacgctcg gagcatttgc cctgatcggc atgttgcgca
     9481 aagaagggca ggagagcgag cagatcgaag attatgccgg gctggccaaa cgcgaaccgc
     9541 tcgccgcgtt tttcatgctg gtgttcatgg tgtcattggc cggcattccc ccgaccgcag
     9601 gcttcatcgg caagttctac atttttatgg ccgcggtgaa cgggggcatg acatggctgg
     9661 ccatcgtggc cgtcatcttc gccgcaattt cagccttcta ctacctgcgc gtcgtgatgg
     9721 tcatgtatat gcgcgaggcc gagggttccg ttgccaccca ttcacgactg gaaacctctc
     9781 cggccctgtc attggttctg gcctgcgccc tcgccggcgt ggtgcttctg ggtctgttcc
     9841 ccaatggcct gtggtcgttg gccagccagg ccgcgccgct cttgaagtag catctcgaac
     9901 cgttcctcat tgcggtggcg tccgcattcc tgcgacgacg tcgcgccggc cttcctcata
     9961 ccgcgccccg gaccgggcca tgtgtcgcag cacaccgact ccacaccagc caggatggca
    10021 tgactgaact gtcgtttctc tccaatgtct tcgacacctt tcgacggcga ggctgtctca
    10081 gcttggccgc ctcgctggcc ttcttctccc tgctgtcgct tttccccatg gtgttcctgc
    10141 tgctctacgg catcagtttc atcgtcagcc aggatgtgat cgggtatcaa tttctcctgg
    10201 ggtttctgaa aggctttctt cccagcatgg gagaacgcct ggccagggat attcgacgcg
    10261 tggcggaaca ggaagaagtc cggtgggcgg tgtttctcgc gttcggctgg ttcagcgcgt
    10321 tggtattcta tgaattggac tacgccatga acacggtatt cggcacggcg gccaagcggc
    10381 atccgctgat ttccacgctg gtggcggtcg cgttgatctg gatgttgggt ttcttgacgt
    10441 tgatctcctt cgtcgccacc caggcgatcg aactcttgac ggcctatgcg ccccgcttcc
    10501 tgggcatgaa cctggtcgcc atcgccgccc atgattttct gatcgcctat tcgctcccgt
    10561 tcctgctcgc gttcgcctcc gtcacctgtc tctatcgatt tcttccgcat cagcggccct
    10621 cgtggcggga ggcgacgatc ggaggggcgg tgttcagcct cttatgggtg tccgccaaag
    10681 ccctgttcgt gacctatctg gaagatgccg ccgtctacac tcaactatac gggtcattgc
    10741 tcgaagtggt gctcctcatg ctctgggtct actattcctc ggcgctggtg ctcctcggcg
    10801 cagtcgtgac ccacgaatgc caatgcagac ggccgtcgta tagcaaagaa gcctctcctg
    10861 atcgggcaga agttgtctga tgcgtaggag aattgggaag caaggaagac cgcgtgtgga
    10921 cgcgttcctg atcctgttat gaggaggaac ccttgccgga tggatgtcgc tgctcaatga
    10981 aatgcccgat agatgcgcgc gtcaccaggc ccaacagttg cccgtcttga acgaccacaa
    11041 gccgctcggc ccccagcgcc aacatctgct ccaacgcctg catcgccgag agatccggcg
    11101 acaccaccat gtcgtcgctg gaggtttgca tgatatcggc cacccggcgg aacgcccaga
    11161 gcccattgct caccgtctgc acatcgcgca cccccacaac accgaccagc cggccgtcct
    11221 gaaccacggg aaatcccccg taaccgtagg ggagaaaata ctggttcacg gcctcctcca
    11281 acgtggtgtc tggcgataag gccacgacat tactgaccat caactcgcgg accggcaccg
    11341 acgccagtga cccgcggata gcggcctgct tccggctccc gcgcgcggcg gcgaacaaga
    11401 acgcccccaa caacacgatc catcccccgc tgctcgccag agcgccggag acggtaccgg
    11461 aaagcgcccc gacaaggagg aaggccccga agaggccgaa gctcacgccg aacaacaacc
    11521 ctaccaatgc cgcctggctt gtcgcgcgat agtagtcctt actccaggcc cacaacccgg
    11581 cccgcaaggc gcgaccgccg tccaggggga aacccgggag cagattaaac aggcccagct
    11641 gagtgttgac catcccgagc agcgtcccca acgccaccaa cccgtacacg gaggatcctg
    11701 tcggaaggaa ctccaagagc gcgaccatgc cgatgcacag accggcgaga agaaagctca
    11761 cgatcggacc ggcaatcgca atcaagaact cagcccgggg atgcggcggt tcctttcgca
    11821 tctgcgccac acccccgaag ataaacagcg tgatctgact gatcggaatc cgataccgaa
    11881 gggccaccag ggagtggccg agctcatgta ggaggacaga cgcaaacagg aggatggccg
    11941 ccacaccgcc catcgcccaa tatcgcggct cggtgagacc gggcagcacg tcgggcaaat
    12001 agcccgtcgc caacgaccag gtcacaaaga aaaagacaaa gagccacgag gcgtgcacgt
    12061 ggatgggaat cccgaacatc cggccgattt tccagtctgg tccctgcatg gtttcaccgt
    12121 agcagccacc cggacagagc gtcaactatc gccgcttcgt gccttcgggt atactgcgcc
    12181 catgcgtggc aagcagttct ggatcagact gtcggcgatc tgcggaatgg cactcgccct
    12241 tcaggctatt ctcgcggagg cgcaggtcat caaggcgggg ccggcttcct gccctgccgt
    12301 ggctttgacc tatgaccttt gccccgtgcg cacggcatcc ggattcgacc gggagctgat
    12361 cgacttcctc atcgagcata aggtgccggc gacgtttttc atgtccggcc gctggatggc
    12421 caagcacgat gccgaggtca agaagctgct cgccgtaccg tttttcgaaa tcggcaccca
    12481 cggcgaggtg catgcccatt tgccgatgca cgaggtcgag gaacagcaac aggaaatcat
    12541 gggaccggtg cgcctgctca agactaaata cggtcgcccg gccaccctat tccggccgcc
    12601 ctatggagaa tataacgacc aaacggttga cgccgtgaaa accctcgggc tcaagttcat
    12661 cctgtggaat atcgaatccg gcgatcccga cccgacgctg acgacggagg cgatcataac
    12721 ccgcattcaa agacgcatga agcctggcag tgtgatcgtc atgcatgcca acggcaaggg
    12781 caagcatacc cgcgaagtga ccgaaaccct ggccgccacc ctgttgccgg ccaaaggcct
    12841 gaccccgatg accgtatcgg acctcttacg atgcaaccaa tcgacagcca cacccgcacc
    12901 ataattcgcg acatgcgagc ggaggatcgc gatccggtga tcgagatcct gaccggctcc
    12961 gacccctgga aacggctcgg cttcactgcc gccgattgga accgcatctt tgcaccgctg
    13021 cccacgggac gagacacatt cgtgctggag atcgaaggga atgtgttggg catcgccatc
    13081 gtgcgcagga aattcctgtt cggagattat ctggaactac tcgggatctc cccgtcggcc
    13141 gtggggcaag gcctgggcag ccggctattg acccacgtcg agtccctgac gtttgcgcga
    13201 gccgttaaca tgtttgcctg tgtgtcggac tttaacgagg gcgcccgggc tttctaccgc
    13261 aagcagggct acaaggaaat cggtcccatg ccgaactttc tcatccccgg ctacgccgaa
    13321 attctgctgc gcaagacgac cgggccggca cgaaagagct aatggctgat agcctatcat
    13381 ttcttctctt gccatcagct atacgccatc tgctataagc tcctcgtatg aaagtcacaa
    13441 aactcctcca cacccgcatg cgggtcagcg acatggatca aacgatccgc ttctatcagc
    13501 acgtattggg cctggaggta ctggagcgca aggtctcgcc gcgtgggtcg cacctcgcgt
    13561 tcctcaaagt acctaacagc gaggaactca tcgaactgtg cagctttccc gccagcggac
    13621 cggtgaccgt gcaggaagac ctggtgcacc tcgcctttca ggtcgagaac ctggacgaca
    13681 cgatccgggc gctggaatcc aaacagatca gaatcaccga cggcccgacc aaaacctcct
    13741 ccggcagccg cttcatcttt atcgacgcgc cggacggcta cgaagtggaa ctcatcgaga
    13801 ggccgccggg cacggcgctg gtgtaagacg cgtcgttcgt gaagcgtcgc tcgtccgaca
    13861 cagacaagtg cacgcatccc cctttcctca cccacgggct gaccccattg acgaatccga
    13921 atccgtccac gtattgtttc tcgcttcacg tttcacgagc gacaaacgac gagaaggagc
    13981 gcgcagatga agaacgggtt tttccgcgga cacggactcg gtaacgacta tgtggtgatg
    14041 gaccccacgg aactgacgtt taaactcacc cccggaaaaa tccgcggcat ttgcgatcgc
    14101 cattggggac tgggcagcga cgggatcctg gcgctggtcc cctcgaagaa agccgacttt
    14161 ggcctgcgca tctacaatcc ggacggcagc gaggcggaaa aatccggtaa tggcctgcgc
    14221 atcttcgccc gctatctgca tgccaccggc aaaacaaaaa agcgagcctt caccgtcgaa
    14281 accaaggggg gcctagtctc cattgccctc catctcgacc gccacggcga tgccgctgcc
    14341 gctacggtcg aaatgggccg ggcgacgttt caaccgagcg ccctcccctg cacactcacg
    14401 gtcgatgaac tgatccagca gcccatcgag gcggccgggc ggtcgttgca attcaccggc
    14461 gtcagcgtcg gcaatcccca ctgcgtgatt ttcaaacaac ctggcgaagc ctggacgaga
    14521 gaggacctcc tcacgctggg gcctgcgctt gaaaaccatt ccctcttccc gaagcggacc
    14581 aacgtccaac ttgcggtgcc caccggcccg aaagacatct ttattctgat ctgggaacgc
    14641 ggcgccggtg aaacgcaggc ctccggatcg tcctcctgcg ccgccgccag cgccgccgtt
    14701 cgtcttggac tggtgaaggg accggtcaca gtgaagatgc ccggcggcac gctcaacatc
    14761 gaagtcgccc aggacttcag tctcacgatg aaaggcccgg tcgcagaggt cgcccgaggc
    14821 gctctgagtc cgtcgtttgt gcgcggactc aaataacttc tcgtcaaccg tcaatggtca
    14881 ctcgtgagga aatacaggaa cccttcctgt tatccttacg cctgatggat gacctatgac
    14941 gacagacgtg ctccaatcca agctcgatca cctgcccggg caaccgggct gctacctctt
    15001 tcggaacgcg aagcgggaga tcctctacgt cggaaaagcc gccgtgttgg cggaccgggt
    15061 ccgctcctac ttccagagag gcagcgacca gacaccaaag accgctctgc tcgtcaacga
    15121 gattgccgac ctcgaaacca tcgtcactcg ctccgagctg gaagccctga tcctcgaaag
    15181 caatctgatc aagcgccacc ggccgcgctt caatatcgtc ctgcgtgacg acaagcagta
    15241 cccctatctg cgcctgccga tcaaagaaca ctttccacgc ctctccatcg tccggcgggt
    15301 gcaaaaagac ggagcgctct actacggtcc ttacaccccg gccggggcgc tccgcgaaac
    15361 actgaaggtg atccgcaaag cgtttccgtt ggccacctgc gaaatcgaga tcgacggccg
    15421 ggccgaccgg gcctgcatcg agttcgagat caaacgctgc atggctccct gcaccggtaa
    15481 ccagtcgcgg gacgaatacc atcagatcgt taaacaggtt cgccagtttc tcgaagggcg
    15541 ggaccgggaa ctgctggaca gcctgcgtga agacatggag cgggcggcgg aacgggaagc
    15601 gttcgaggag gccgcccggc tgcgggaccg actcttcagc gtcgaacgca cgttggaaaa
    15661 acaacg
//
LOCUS       DAOQRM010000051        15541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2886_length_15541_cov_20.171510, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000051 DAOQRM010000000
VERSION     DAOQRM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2886_length_15541_cov_20.171510"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..475
                     /locus_tag="PKK81_06055"
     CDS             128..475
                     /locus_tag="PKK81_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV32469.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAEGKGNHVGDGQGV
                     GDNIYVITHDRCTECVGHFDEPQCAAVCPVDNCCIADPAYPETTEVLLDKAKTLNPDK
                     EIDPAKVWSGVRN"
     gene            680..1015
                     /locus_tag="PKK81_06060"
     CDS             680..1015
                     /locus_tag="PKK81_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32470.1"
                     /translation="MTRVSRLILFASLGLALLPVQTMADTGDIGTIIESFVTKQFPDA
                     ANHFWIVNDTQWDGDEMIVDMNAIVTERRQDVPTASRFLLLIVDGRLRGIQNIPLDAE
                     AECQKEQEA"
     gene            complement(1122..1601)
                     /locus_tag="PKK81_06065"
     CDS             complement(1122..1601)
                     /locus_tag="PKK81_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32471.1"
                     /translation="MGKTMVAVFFGLVMLVAGASGAYALQAGGVEIGDLDTGSVVGQP
                     FKELVVDAELYAVEIGNMKAWYPPVTVIDFKVRPGRPVVLKVTNNSTAEHGFQMTDAA
                     NSGSPFVMKAEVVLKPGETKYIGVPTSDMFYATQGNTLTYRCHLHPAHVGGKILMIK"
     gene            complement(1877..3421)
                     /locus_tag="PKK81_06070"
     CDS             complement(1877..3421)
                     /locus_tag="PKK81_06070"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNV32472.1"
                     /translation="MPRLFLLITALVTGAIVMALEILGSRLLAPVFGNSLFVWGALIG
                     IILAAMSSGYAFGGWASDRYRVAAVLAWLLLGSGAWTLLMSWMGQVAIFKVAKLVEDP
                     RWGPSLAACVLLAPPAFGLSGVMPALLRLAVVDMGYLGRHTGSMIALSTVGSLAGTWG
                     TAFFLLSWLGTQTLVASLGVLQLLLGLLWLQQGSGKVTKVTGLLLAGLLVLSWQLFGQ
                     AAPVAGLVYQEDSPYQQVRVRDDDLFRYLVLDRTWHAVMWRSDPATLFLPYSQLMVAA
                     VALTPDPKRGLILGHGGGSLAKWLAQVWPELELDVVEFDPVVVRMAQEYFEYHPPANH
                     HVFVKDARVFVRDTGVKYDVIWVDAFARHLIPFHLTTVEFFSELRSRLNPNGVLALNL
                     ASSGEGGDLQRASAVVQTLKTAFPTLESFGVKGPWRAHQTTAENLIFFGGGPIDTMPY
                     DEVVKRIQSHVEARRLPPEATALLAARRTQPWSPGLTLTDDYTPYDLLIGSHAVEMSP
                     EGKALP"
     gene            complement(3515..3886)
                     /locus_tag="PKK81_06075"
     CDS             complement(3515..3886)
                     /locus_tag="PKK81_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV32473.1"
                     /translation="MMMRPITRTVHPDDTLLAVARQLRDARVGAMLVADHGDYVGIVS
                     EADLVRKAMASGADAEQVMARSVMSTPVMTIDIAQSAHEASDVMAERGIRHLVITEEG
                     RMVGMISVRDLLRYFKNWGTL"
     gene            4116..5435
                     /locus_tag="PKK81_06080"
     CDS             4116..5435
                     /locus_tag="PKK81_06080"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNV32474.1"
                     /translation="MHISVIGTGYVGLVTGACFAEFGVNVTCMDTDARRIARLEKGEV
                     PFFEPGITELVAKGIKEDRLHFTTDVAKAVDKALVIFIAVGTPPKSDGSADLSYVEEV
                     GRGIAKNMTGYKVIVTKSTVPVGTGEKLREVIKANQTGRFRFDIVSNPEFLREGSAIE
                     DFMRPNRVVIGADSEQAVAIMKDLYRPLYLLETPIVVTDIPTAEMIKYASNAFLAVKI
                     SFINEIATVCEKVGADVQMVSKGMGLDNRIGNKFLHAGPGFGGSCFPKDLAALVQTGE
                     RVGYPFQIAGAAAKVNYEQHLRMVEKVKDACGGVKGKTLGVLGLSFKPNTNDMREAPS
                     LTILSELMKEGATIRAYDPASMEESMKLLPGMVPCQDTYDVAEGADGLIIMTEWNQFR
                     NLDFDRLKKSMRQPLLLDLRNTYESDRVVGFGFRHVSVGRPTKNPAA"
     gene            complement(5457..6167)
                     /locus_tag="PKK81_06085"
     CDS             complement(5457..6167)
                     /locus_tag="PKK81_06085"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV32475.1"
                     /translation="MAEEAPVKLIQIGPKGGTKKDGFNLVTERVVAVNPEAKQLEVEL
                     LAYDGKTVVLDVGDEALEDFLKIKPGDGATIRVVEEGGKRIAKSFRIRAKDPNAAKAD
                     AMLIDLKDSHWLNRKYAAEVLGELKDPRAVLPLVEALTDEVGDVRQRAYDSLIKIGGI
                     AVASLVPLLASEEDDVRQSATEIIRKIGKPAVEPLATALADADDRLKTRIMKVLDRMG
                     YKPKAKEGAQAEPAKLLS"
     gene            complement(6188..6949)
                     /locus_tag="PKK81_06090"
     CDS             complement(6188..6949)
                     /locus_tag="PKK81_06090"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV32476.1"
                     /translation="MSDAERIAQLISALRDDNEALRDHAMASLGQMGIEAVPQLIGLM
                     ADEDVVIREAAATAVVRIGPVAFDQMLEALSDDEWAIREQAANALGRFRDPRAVDPLM
                     VALKDKDGAVRTAAVWALERIGDSRATPGLIEALGDGTVREDVARVLKKIGDTRAVDA
                     LIGGLLGPNWMVRRHAAEALGKIGDPRSADALIQSLQDEDWLVRRNAAESLARLGAKQ
                     AIEPLLPLLEDENTMVQETVEGVLASLGWKQATSS"
     gene            complement(6942..8042)
                     /locus_tag="PKK81_06095"
     CDS             complement(6942..8042)
                     /locus_tag="PKK81_06095"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV32477.1"
                     /translation="MSKESIDTLVSELTHEEEWRRMRATAACLAGGPRAVQALTQALE
                     SGDVALRVEAAAMLSRIKDPAAGLSLVGLLRDSEEAVRQAAFSALEQMAGNLDDETAA
                     GLVRNLHESPDEDVRSRVRQLLGVIPNAITPLCEMLTHPEVDAQTTAATILEHLLDPR
                     SADGLIDAMSSPAVRDTAVRTLKKLGAIRDRIDATFNALRDVEGASEREEARMSTVMD
                     LLGIGRPAVEILIEYLEDDDWVVREAAADLLGKIGDVRAVEPLMKRLQLDKDTGVKEY
                     AVKSLGLIGDPRPAQLYIEAIPIRPLRVMAIEALAKIKDVEVLRPYNELFNRLRTDRD
                     GIVSYSAGLIADKLAALEGEQPVGQEESDDHE"
     gene            complement(8138..9220)
                     /locus_tag="PKK81_06100"
     CDS             complement(8138..9220)
                     /locus_tag="PKK81_06100"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV32478.1"
                     /translation="MADTVSEQIAALSDEDWAIREEAATLLGSLNDARAVLPLTKLLR
                     DADRAVREAAIGSLSALGSASVPALAGCLADPALQVQEAASAILASIADARVFRPLVE
                     ALGSRDWIVRMHAAKGLGRIGDPEAVSVLMPLLQDKVKAVREEASSALAAIGAAAVLG
                     LIQALEHDDWLVRLHAVEALGKLKSPDSVDPLLRALFNERDSAIREDVVRALGAIRDA
                     RAVDYLVTVMKEPGLRLLAVEALGHIGDPRAVPLLRRVVEGVPLGEPRDTATPCADGW
                     TDEMATMGMAARALGMIADGVAIPSLIVALRNTITRSEAAAALAKFGPAVIPSLLPML
                     AKEQDENIRYHVRETLTAVGWRPGRV"
     gene            complement(9408..10778)
                     /locus_tag="PKK81_06105"
     CDS             complement(9408..10778)
                     /locus_tag="PKK81_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV32479.1"
                     /translation="MAQALDDLLDSLEDADDATREEAAKALAELANPATLDALIGACG
                     DEFWSVRTRAGWGVAKIGGPKAIEALIVLFNDPIMEVRNEAVAAVVSLGVGQLDRLLA
                     AMKDERWRVREHAAKACGDLHDSRAVDALIFACRDRDGAVKSAAAEALGKIGDPKAIP
                     ALVKLFRDSSKIVRETAGIALVAIGQPSVDLLLETLKDKDFVVRCHAARALGGMTTDY
                     QIGRSWVREPRVVDALIEALKDPDRAVREDATIALGMIGDPRAIDGLLEAMKDGAVKR
                     HAIASLGMIGDPRALPAVLAALKGKGVRQDGTPTPGCIVSEDAFIKEAAATALGHFRD
                     PRVIPDLIMLLKDGVLREKAAAALVLIGDSAIEPLISFLYDPKASEVEAEGERVLSYA
                     SVRLTAKDSLRLLVVETLEHLGWTPPDEETSVDSSQADNLRVDRPLGDIGRFGPSGDF
                     AKGSVR"
     gene            complement(10759..11250)
                     /locus_tag="PKK81_06110"
     CDS             complement(10759..11250)
                     /locus_tag="PKK81_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32480.1"
                     /translation="MRSSSHKAFAGRQVFFWIVAVTCVLVLGPSERLLAAGPPPAYGK
                     EHTITIDPTPLVPQSWWQVPGVTPPLWVLDPETSDAYRTTETRELKLKPGQYKFISFT
                     FDFPFTVSLDGKLEYSKSLDQCVEGRGTQTLVVRCKRTYPHGGQRDAYYGNSEVGDGT
                     GTR"
     gene            complement(11258..12307)
                     /locus_tag="PKK81_06115"
     CDS             complement(11258..12307)
                     /locus_tag="PKK81_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNV32481.1"
                     /translation="MSRIRSIMTVVAVLCMVVGLGPALVAPSSQSVAFAARGGGEVAT
                     PVQAGITEHVILFVLEGLGQESLKSGAMPVLSSLVKDGAVTWSATAVAPARRLPTMAS
                     LVTGMPVAKHGITWNVFEFSRGYPRAPTVFDYLDLSGGRDSAIFYMDESLYQLAKPEP
                     YTDYQMCGPLRAECNPDRLVGYVRDYFKKATSGSGYGHAIPALPHLLVVHLPAPGRVG
                     ETQGWKSVAYKDALKAVDKAMGTVLDLYREHGLIKRTTVFATSLSAFGETQFSAGEVS
                     GEQAGNVPVVPWIASGVGIKAGHSIRQPVSIIDTGATVMRALGLTTYTEWESHPVEEI
                     FKTAFAAAPVSPLLQ"
     gene            12493..13674
                     /locus_tag="PKK81_06120"
     CDS             12493..13674
                     /locus_tag="PKK81_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32482.1"
                     /translation="MPQNRFDSGPRFIPHTSPRARSIHLFIGLLSIALSLLLTCPSPS
                     QAEVRVVTGTGEHRMNDHETKTDGIRLATEQAKKQALEQVASYLESVTVVRDLDVTLD
                     EIRSYTAGMVLVLDQHVTTALNGEFVVVRVELTAQVDTDEVAQAIATLRQNEDAREQL
                     LALRRDVESLQQELDAANDTLAAATSPEQVQELSHERNELLNRAESDAMVAQAWTDWV
                     LLTPFAQPSPSAGLAQIHALLGLASRLNPDNPHLAIAQQTVGTKAPPAPPQPRRPPVP
                     HTVPFLPGYAVVPQQPAQPGAIQVPAQPGAPLPQGSLGTMPTYRRVPPPSGRPAARTL
                     HTYRSGVDQATPAAEQPSINAPRGTPPAIIYQVPKKPVPIPTTPSQQIPGNGTHQGGT
                     R"
     gene            13674..14231
                     /locus_tag="PKK81_06125"
     CDS             13674..14231
                     /locus_tag="PKK81_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32483.1"
                     /translation="MSLTAGPIRTGWWPLCALLIASPATAADLTPPAVRDHADQTFQH
                     LQMQGRAESPTSAAQARPAQATSSSEELLTGQGQGDLGKGKLVCQRVSELAARADIAK
                     QIRVLVKEHATDRLRERTGREVEQDIEVVREEIVQEYLQGVRIVERHTDEEAKTCSAT
                     AVMPKPRLSTPASTESNGREPAAHR"
     gene            14300..>15541
                     /locus_tag="PKK81_06130"
     CDS             14300..>15541
                     /locus_tag="PKK81_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32484.1"
                     /translation="MPIENNFQHDELSRKNPGDRLTYADWTTPPDADSPAWQEAMSQL
                     GQKLSQAGVRLIMFLHGAIPGTDVFGLGRLDEVGGLKRGYSRGISGLDSLLSFMREGT
                     NGITPLPGGMKPPLSNDEATKTLLDSQIGDAGNFTTTTVEHCRKALNQKLATPITCIR
                     ELWSSEHHHLGRAMAACRLLDRLRALVIEQHLGAGDRILIQAHGQAGLVLALASNLLC
                     PSPITGRKTLFDLLLAANPQGPDAQAIQRIDPLLTNGTLLNGVALDIVTFGTPVRYGW
                     DPSGIGKLLHVVNHRNLRTDGKTWLSKMDLPQITVEMPIAWGGDYVQELAVAGSDALP
                     AENAKAANKAVWELLEPYDGFERWVECARRAGRFPSEGQCLLVDYKDCTSSTNVRDHY
                     YGHAAYTRTNAMLFNFNQIVGR"
BASE COUNT         3405 a         5101 c         4210 g         2825 t
ORIGIN      
        1 tctgttgact tgccgaagaa cggcggcata gaatgcggac ctgtttcgcc gcgaactatg
       61 gtcgcggcgg cacgaaattg tcaacatctt atccgtcatt aaagaaactg agaaggaggc
      121 acaggccatg gcgcttctga tcaccgatga gtgcatttcc tgcggggcat gcctgccaga
      181 atgtcccaat gaagcgatct ttgaaacccg cagcgatgct gaaggcaagg gcaaccacgt
      241 cggcgacggt cagggagtcg gtgacaacat ttatgtcatc actcatgatc gttgcacgga
      301 atgcgtcggg cactttgacg aacctcagtg cgcggcggtc tgcccggtag acaattgctg
      361 cattgccgat ccggcctacc ccgaaaccac cgaggtgttg ctcgacaagg ccaagacctt
      421 gaatccggat aaagaaattg atccggccaa ggtgtggagc ggggttcgaa actagtcaga
      481 atacgtcatt ctcctatagg tgacaccacg aaggctcctc cgggagcctt cggcgtttct
      541 gcgctgcaaa agagataact ccctggcttt cactggccaa cagctgtaga ctttaagagc
      601 gaggcggcct gcgcaggccc ccacccttaa ggccttgctg cactccgccc attcgtcacc
      661 ctctcagcgg gaggtgctca tgacacgcgt aagtcgcctc atcttattcg ccagcctcgg
      721 cctcgccctg cttcccgtcc aaaccatggc cgataccgga gacatcggaa ccatcatcga
      781 aagcttcgtc acgaaacagt ttccggatgc agccaatcac ttctggatcg tcaatgacac
      841 gcaatgggac ggcgacgaaa tgatcgtgga catgaacgcc attgtcaccg agcggcggca
      901 ggatgtcccc acagcaagcc gctttttgct actcatcgta gacgggagac tcagaggcat
      961 tcagaatatt cctttggacg ccgaagccga atgccaaaag gagcaggaag cctaggaagg
     1021 aaacatttta tcgataccag tcggcgatgc gcctgataca acaacgcccc atctgcatca
     1081 ctgcagatgg ggcgttgcac tacacgtatt tgaaacgtcg actacttgat catcaagatc
     1141 ttgccaccga cgtgggccgg atgcaaatgg cagcgatagg tcaacgtgtt accctgggtg
     1201 gcataaaaca tgtcactggt gggaacaccg atgtacttgg tttctcccgg cttcaacacg
     1261 acttcagcct tcatcacgaa cggggaaccg gaattcgcag catcggtcat ctggaaccca
     1321 tgctctgcgg tcgagttgtt cgtgactttc aaaacgaccg gacggcccgg acgaacttta
     1381 aagtcgatca ccgtgacagg cggataccac gccttcatgt tgccgatttc gacggcatac
     1441 aattccgcgt ccacgaccaa ttccttaaac ggctgaccga cgacagaacc ggtatccaaa
     1501 tcaccgattt cgacaccacc agcttgaagc gcatatgcgc ctgatgcccc agccaccagc
     1561 atgaccaggc caaaaaacac tgctaccatc gtcttaccca tgacctacct ccttaaagag
     1621 attaagaaga gatgcgatta ggttggtgaa taatgaccac cgcgctctga accatctgct
     1681 agacactccg cccaggagcc tagcctctac tactcgaaaa tgtcctaaat aataggctga
     1741 tgagggacac cttatagcat agggattaaa aaggaagcaa gaaagttctt ggacgtgaag
     1801 cgaacctagt taccccacga aatgtcttac ggaccaaaga ctcgacaggc ttcattccat
     1861 tcataatttc atatgtttac ggcaaagcct ttccctccgg cgacatttct accgcatgcg
     1921 atccgatcag caaatcgtag ggcgtatagt cgtcggtcaa ggtcaggcca ggcgaccagg
     1981 gctgggtgcg tcgcgccgcc aacaaggcag tcgcttcggg tggcagtcgc cgagcttcga
     2041 catgcgattg aatacgcttc acaacctcgt cgtacggcat agtatcgatg ggaccgccgc
     2101 cgaaaaaaat caaattctcc gccgtggttt gatgcgcccg ccaaggccct ttcactccaa
     2161 aggactcgag tgtgggaaac gccgtcttca acgtctgcac gaccgcgctc gcacgctgga
     2221 gatctccacc ctcgccggat gacgccagat tgagggccag cacgccattg ggattcagtc
     2281 gactccggag ttctgagaaa aactcaaccg tggtcaaatg aaaggggatg aggtgccggg
     2341 caaacgcatc gacccagatg acgtcgtact tcacccctgt atcgcgcaca aacacacggg
     2401 catctttgac gaagacatga tggttcgcgg gggggtgata ctcaaaatac tcctgggcca
     2461 tccgaacgac gaccgggtca aactcgacga catccagctc caactccggc catacctggg
     2521 ccagccactt ggcgagtgat ccaccaccgt gccccaaaat gagtccgcgt ttggggtccg
     2581 gggtcaacgc gaccgctgca accatgagtt ggctataggg aagaaagagc gtggcagggt
     2641 cggaccgcca catgaccgca tgccaggtgc ggtcgaggac cagataacga aacagatcgt
     2701 cgtcacgaac acgcacctgc tgataagggc tatcctcctg gtacaccagc ccggcaacgg
     2761 gagccgcctg cccgaataat tgccaactca aaacaagcag accggccagc aacaaacccg
     2821 tcactttcgt gacctttccg ctcccctgct gcaaccacaa caaacccaac aaaagctgga
     2881 gcacacccaa tgaagcgacg agcgtctggg tccccagcca ggagagaagg aagaacgccg
     2941 tcccccaggt cccagccaga ctcccgacag tggacagcgc aatcatgctg ccggtatggc
     3001 ggccgagata ccccatatcg accacggcca accgaagcaa tgccggcatg acgccgctca
     3061 atccgaatgc cgggggggcc aataacacgc aggccgcaag gctggggccc caccgcgggt
     3121 cctcgacaag cttggcaacc ttaaaaatgg caacctgccc catccaggac atcagcaacg
     3181 tccaggcgcc ggaaccgagc aggagccagg ccagcactgc cgcgacacga taccgatccg
     3241 acgcccagcc gccaaacgca taccctgaac tcatggccgc caggatgatc ccgatcaagg
     3301 ccccccagac aaacagcgaa ttcccgaaca ccggagccag caaacggctt cctagaattt
     3361 ccagcgccat gacgatggca ccggttacga gcgccgtgat gagcagaaac agacgtggca
     3421 tgcgagaggc ggaaggtccc tgtgccatca tcaacacagg accacagaaa ggctagaagc
     3481 aaaacagtgc cgttcggaat cgaggcacgc catattagag cgttccccaa ttcttgaagt
     3541 agcgaagcag atcgcgcacc gaaatcatcc ccaccatacg cccctcttct gtaatcacga
     3601 gatgccggat tccccgctcg gccatcacat cgctcgcctc atgcgcggat tgggcgatat
     3661 cgatcgtcat gaccggggtg ctcatcactg accgggccat gacctgttcc gcgtctgcgc
     3721 cgctggccat ggcttttcgg acaagatcgg cctcgctgac aatccccaca tagtcaccgt
     3781 gatcagcgac cagcatcgcc ccgacgcggg cgtcgcgcag ttgcctggcc acggccaaca
     3841 aggtgtcatc gggatggacg gtcctcgtga taggacgcat catcatcgct aatggtcgtt
     3901 ggatgggccc gcctgaacct gccattcgcc agcctctcct tcgaagaggg ccgaattcta
     3961 ctggatgtgc gcggcaatct gcaattcgcc ccgtccgcac ccctgccaaa acctgcgtta
     4021 tgtttgctca ctttcgtacg cgttgctata ctaccgactc tacacacgta cttacccaca
     4081 gtttcgacgg cttatcttca ggaggagatg cttccatgca tatcagcgtg attggaacgg
     4141 gctatgtcgg cttagtcacc ggtgcctgct tcgcggaatt cggcgtgaac gtcacctgta
     4201 tggacacgga tgcgcgccgc atcgccagac tcgagaaggg tgaagtcccg tttttcgaac
     4261 ccggtatcac cgaactcgtc gccaaaggca tcaaagaaga ccgccttcat ttcaccaccg
     4321 atgtcgccaa ggccgtcgac aaagcgctgg tcatcttcat tgccgtcggc acgcccccga
     4381 aatcggacgg ctcagccgat ctgtcctatg tcgaagaagt gggccgtggc atcgccaaaa
     4441 acatgaccgg ctacaaggtc attgtcacga agtccaccgt ccctgtcggc accggcgaaa
     4501 aattgcgtga agtcatcaag gccaaccaaa cgggccgttt ccgttttgac atcgtctcca
     4561 atccggagtt cctccgcgaa ggatccgcca ttgaagactt tatgcgtccc aaccgcgtcg
     4621 tgatcggcgc ggacagtgag caagcggtcg cgatcatgaa agacctctat cgtccgctgt
     4681 acctgctgga aaccccgatt gtcgtaaccg acatcccaac tgctgagatg atcaagtacg
     4741 cctccaatgc gtttctcgcc gtcaagattt ccttcatcaa cgaaatcgcc accgtctgcg
     4801 aaaaggtggg cgccgacgtg cagatggtct ccaaaggaat gggcctcgac aatcgcatcg
     4861 ggaacaaatt cctgcatgcc gggccgggat tcggaggctc gtgttttccg aaggacttgg
     4921 ctgcactggt ccagaccggc gagcgcgtgg ggtacccgtt ccagatcgca ggggccgcgg
     4981 ccaaggtgaa ctacgaacaa cacctgcgga tggtcgagaa ggtgaaggac gcctgcggcg
     5041 gcgtgaaggg aaaaacgctc ggcgtgttgg gcctgtcgtt caaacccaac accaatgata
     5101 tgcgggaagc cccgtcactg accatcttga gcgaactgat gaaggaaggg gcgacgattc
     5161 gcgcctacga cccggcctcg atggaagagt cgatgaaact tttgccgggg atggtaccct
     5221 gccaggacac ctacgacgtc gcagaaggag ccgacggctt gatcatcatg accgagtgga
     5281 accagttcag gaatcttgat ttcgatcggt tgaagaaatc catgcggcaa ccactcctgc
     5341 tggatctccg caatacctat gaatcagacc gcgtggtcgg cttcggattc cgccacgtct
     5401 cggtcggtcg tcccaccaag aatccggccg cctaaccggc ggccggacgg ctcacactaa
     5461 ctgagcagct tggcaggctc ggcctgagca ccctctttgg ccttcggctt atagcccatc
     5521 cgatcgagca ccttcatgat tcttgttttc agccgatcat cggcatccgc cagggctgtg
     5581 gcgaggggct cgacggccgg cttaccgatc ttccggataa tttccgtcgc cgactggcgc
     5641 acgtcgtctt cctcagaagc caacaacggt acgagtgacg cgacggcgat cccgccgatc
     5701 ttgatcaaag aatcataggc tcgctgccgc acatcgccca cctcgtcggt cagggcctcg
     5761 acgagcggca gaacggcgcg cggatctttc aactcaccca gcacttccgc cgcatatttg
     5821 cgattgagcc aatgggaatc cttgaggtcg atcaacatgg catcggcttt ggccgcattc
     5881 gggtctttgg cgcgaatccg gaagctcttt gcaatgcgct tgccgccctc ctcaaccaca
     5941 cggatcgtcg ccccatcgcc gggcttgatc ttgaggaaat cttccagggc ctcatcgccg
     6001 acatccaaca caacggtctt tccgtcgtac gcaagcagct ccacctccaa ctgcttggct
     6061 tcgggattga cggccaccac gcgttccgtc accaggttga acccgtcctt cttggtcccg
     6121 ccctttggtc cgatctgaat cagttttacc ggtgcttctt ctgccatagt agagttcctt
     6181 tatcgggtta tgacgaggtc gcctgtttcc atcccaaact cgcaaggaca ccttcgacgg
     6241 tctcctgcac catcgtgttt tcgtcttcca ggaggggaag cagcggttcg atggcctgct
     6301 tcgcccccaa gcgcgccaac gattcggcgg cattccgacg cacgagccag tcctcatcct
     6361 ggagcgactg aatcaacgca tcggcgctgc gtggatcacc gatcttgccg agtgcttccg
     6421 cggcatggcg ccgcaccatc caattgggac ccagtagtcc gccgatcagc gcatcgacgg
     6481 ccctggtatc accgatcttt ttcaagaccc tcgctacgtc ctctcgcacc gtcccgtcgc
     6541 cgagcgcctc gatcaatccc ggcgtcgcgc gggagtcgcc gatccgttcc agagcccaga
     6601 cggccgccgt ccggacggct ccgtccttgt ctttcagggc caccatgagg ggatccaccg
     6661 cacgcggatc gcggaaacgt ccgagcgcat tcgccgcctg ctcgcgaatc gcccactcat
     6721 cgtcactcag cgcctccagc atctggtcga acgccacggg cccgattcgc accacagccg
     6781 tcgcggccgc ttcgcgaatc accacgtctt catcggccat caggccgatc agctgtggaa
     6841 cggcctcgat gcccatttgc ccgaggctcg ccatcgcatg gtcccgcaac gcctcgttgt
     6901 catcgcgtag cgcggagatt aattgtgcaa ttcgctcggc atcactcatg atcgtccgat
     6961 tcctcctgtc cgaccggctg ttcgccttca agcgctgcaa gtttgtccgc gataaggccc
     7021 gcgctgtacg acacgatgcc gtcccggtcg gtccgcaacc ggttgaacag ctcgttatac
     7081 ggacgcaaca cctcgacgtc cttgattttc gccaaggcct cgatcgccat cacccgcaag
     7141 ggcctgatcg ggatcgcctc gatatataat tgcgccggac gcgggtctcc gatgagcccc
     7201 agcgacttca cggcatactc tttcactccg gtatccttat ccaactggag ccgcttcatc
     7261 aacggctcga ccgcgcgcac atccccgatc ttccccagca agtccgcagc cgcttcacgc
     7321 accacccaat cgtcatcttc cagatactcg atcaaaattt cgaccgcagg tcgcccgatc
     7381 cccagtaaat ccatgaccgt ggacatgcgg gcttcttcac gctcgctcgc cccctccacg
     7441 tcacgcaagg cattaaacgt cgcatcgatc cggtcccgta tcgcccccag cttcttcaac
     7501 gtacgcacgg cggtgtcacg aaccgcggga gaggacatgg catcaatcaa tccgtccgcc
     7561 gagcgcggat caagcaagtg ttcgaggatc gtcgccgcgg tcgtctgcgc gtcgacctcc
     7621 ggatgggtca acatttcgca caagggagtg atggcgttgg ggatcacacc caggagctga
     7681 cgaacccggc tcctgacatc ttcatctgga gactcgtgga gattgcgcac cagacccgcg
     7741 gctgtctcgt catccagatt gccggccatt tgttccagag ctgaaaaagc ggcttgccgg
     7801 acggcttcct cggaatcccg aagcaaaccg accaaagaaa gtccggctgc cggatccttg
     7861 atccgcgaca acatcgccgc cgcttcaacg cggagagcaa catcgcccga ttccaaggcc
     7921 tgcgtcaacg cctgcaccgc ccgaggccca ccggccaagc aggcagctgt ggcccgcata
     7981 cgccgccact cttcctcgtg ggtcagctca gaaacgagtg tatcgatgct ttctttagac
     8041 atgtgttcgt tggcgagtgc gaagttttct gattcggtga gtgccagagg cgaagatcag
     8101 ccgtggctac ctcaccacat cacgaactaa taccctgtca gacccgtccc ggacgccagc
     8161 cgacagccgt caacgtctct cgaacgtgat accgaatatt ctcgtcctgt tcctttgcca
     8221 gcatcgggag cagggacgga atcaccgccg ggccgaactt ggccaaagcc gcagccgcct
     8281 cagaccgggt aatggtattc cgcaaggcca cgatcagaga cggaatagcc acaccgtcgg
     8341 cgatcatccc caacgcgcga gccgccatcc ccatggtcgc catctcgtcc gtccacccgt
     8401 cggcacaagg ggtggccgta tcgcgaggct cgcccagcgg taccccctcc acaacccgcc
     8461 gaagaagagg cacggcacga gggtcaccga tatggccgag cgcctcgacg gccagcagcc
     8521 gcaaccccgg ttccttcatc accgtcacca gataatctac cgcccgcgca tcccggatcg
     8581 cacccaaggc ccgcaccaca tcttcgcgaa tcgccgagtc ccgctcattg aacagggcac
     8641 gcagcaacgg atcgacagaa tccggagact tcagcttacc caacgcctcc acggcatgga
     8701 ggcggaccaa ccagtcatcg tgttccaggg cttggatcaa ccccaacaca gcggcagcac
     8761 cgatggcggc cagtgcgctg gaggcctcct cacgaaccgc cttgaccttg tcctgcaaga
     8821 gcggcatcaa gacgctgacg gcttcgggat cgccgatccg tcccagcccc ttcgccgcat
     8881 gcatgcgcac gatccaatcg cgactgccca gggcctcgac cagcgggcgg aaaacccgcg
     8941 catcggcaat cgaggccaaa atggccgaag ccgcttcctg cacctgcaac gccggatccg
     9001 ccaagcagcc ggccagagcg ggaaccgacg cggagccgag cgcggagagc gacccgatcg
     9061 ccgcctcacg gacagctcga tcggcatcgc ggagcaactt cgtcagcggc agaactgccc
     9121 gggcgtcgtt gaggctgccc agcaaggtcg cagcttcttc ccgaatcgcc caatcttcgt
     9181 ccgacagcgc tgcgatctgt tcactgacag tgtcggccat ggtacctcgg cccctggtga
     9241 ggccatcgac cttaacacag gattttttga ggggtcaacg cagggtcggc cgtgcgacct
     9301 ggcatcgaac cgttcccgcg catctggtct aaacagagga gagtagccgg taggagcgcc
     9361 ggagatgctc atccctgggg acggaccccg ggggaggaag ccggtgatta ccgcaccgat
     9421 cctttcgcaa agtctccgct cgggccgaac cgtccgatgt cgcccagcgg gcggtcgaca
     9481 cgaagattgt cggcctggct ggaatccacc gacgtctctt catccggagg cgtccaccca
     9541 agatgttcca gtgtttcaac aacaagtagc cgcagggaat ctttggccgt cagccgcacc
     9601 gaggcgtagg acaggacgcg ttcgccctcg gcttcaactt ccgacgcttt cggatcatag
     9661 agaaaagaga tgagcggttc gatagccgag tcaccgatca gcaccagcgc cgccgccgct
     9721 ttctcacgca acaccccgtc tttcaacagc atgatcagat cgggaatcac gcgcggatcg
     9781 cggaagtggc cgagcgccgt ggccgctgct tccttgataa aggcgtcttc actgacgatg
     9841 cacccgggcg tcggagtgcc atcctgacgc acaccttttc ccttgagcgc cgccaagacg
     9901 gcaggaaggg ctcgaggatc gccgatcata cccagcgagg cgatggcatg gcgcttcacg
     9961 gcaccgtctt tcatcgcttc caataacccg tcgatggcgc gtggatcccc gatcataccc
    10021 aacgcaatcg tggcatcttc ccgcacggcc cggtccggat ctttcagcgc ctcgatcaag
    10081 gcatcgacga cacgcggctc ccgcacccag ctccggccga tttgatagtc cgtcgtcatg
    10141 cctcccaatg cgcgagccgc atgacaacgc acgacgaaat ctttgtcctt caacgtctcg
    10201 agcaacaaat cgacggaggg ctgtccgatc gccaccaggg caataccggc ggtttcacgg
    10261 acgatcttgg atgaatcacg gaataatttc accagcgcgg gaatggcttt cggatcgccg
    10321 atttttccca gcgcttccgc ggcggcactc ttcacagccc cgtcgcgatc ccgacaggca
    10381 aatatcaacg catcgacggc gcgcgaatca tggagatccc cgcaggcttt ggccgcatgc
    10441 tcccgaaccc gccaccgctc atccttcatc gcggcaagca gccgatccag ctggcccacg
    10501 ccaagcgaga ccacggccgc aaccgcctcg ttgcggactt ccatgatcgg gtcgttgaag
    10561 agcacgatca gcgcttcgat cgccttcggt ccgccgatct tagccacgcc ccagccggca
    10621 cgagtccgca cagaccaaaa ctcatcgccg caggcgccga tgagcgcatc cagcgtggcc
    10681 gggttcgcta actcggcgag agctttcgcc gcctcttcac gcgtggcgtc gtccgcatct
    10741 tcgagggaat ccagcagatc atcgagtgcc tgtgccatca cccacctcac tattcccgta
    10801 ataggcatca cgttgaccgc catgaggata ggtgcgtttg caccgtacca ccagggtctg
    10861 agtcccgcgg ccctccacac attgatcgag tgacttggag tattccagtt tcccgtccaa
    10921 actcaccgtg aaggggaagt cgaaggtaaa actgatgaac ttgtattgtc ccggcttgag
    10981 cttcaactcg cgcgtttcgg ttgtccggta ggcatccgag gtctccgggt ccaagaccca
    11041 gagcgggggt gtcacaccgg gcacctgcca ccaggattgc ggcaccaatg gagtcggatc
    11101 gattgtaatc gtatgttctt tgccataggc agggggagga ccggccgcca agagccgctc
    11161 ggatggccct agaaccaaca cacacgtcac ggctacgatc cagaaaaaca cctgccgccc
    11221 ggcgaatgct ttgtgcgatg atgaacgcat cggtcctcta ttgcaggagt gggctgacgg
    11281 gagcagcagc gaatgcggtc ttgaagattt cttcgaccgg gtggctctcc cattcggtat
    11341 acgtcgtgag tccgagcgcg cgcatcaccg tcgcgcccgt atcgataatc gacaccggct
    11401 ggcgaatcga atgcccagcc ttgatgccga cacctgaggc aatccatggc accaccggaa
    11461 cattgccggc ctgctcaccg gagacctcac cggccgagaa ttgcgtctcc ccgaaggcac
    11521 tcagcgaggt ggcaaacacc gtggtccgct tgatcaaacc atgttcccgg tacagatcga
    11581 gcacggtccc catcgcctta tcgaccgcct tcaacgcgtc tttgtaggca accgatttcc
    11641 agccctgcgt ctctccgaca cgcccgggag ccggcagatg gaccaccagg agatgcggca
    11701 aggctggaat ggcatgacca taccctgaac cactcgtcgc ctttttgaaa taatcccgga
    11761 cgtagccgac cagacgatca ggattacatt cggcccgcag cggaccacac atttgataat
    11821 ccgtgtaggg ctccggcttc gcgagttgat acaacgactc atccatatag aaaatggcac
    11881 tgtcccggcc gccgctcagg tccagataat caaacaccgt cggcgcgcga ggatatccac
    11941 ggctgaattc aaagacgttc caggtgatcc catgtttggc gaccggcatg cccgtcacca
    12001 gcgaagccat cgtcggcaac cggcgcgcag gggccactgc agtagcagac caggtcacgg
    12061 ccccgtcctt cacaagggac gacaacaccg gcatcgcgcc actcttgagt gattcctggc
    12121 ccaacccttc caatacgaag agaatcacat gttctgtgat gcctgcctga acgggagtgg
    12181 ccacttcgcc cccaccgcgt gccgcaaagg caacggattg ggacgagggc gccacgagtg
    12241 ccggccccag accgacgacc atgcagagga ctgccacgac tgtcataatg ctgcgaatac
    12301 gactcataac ctgccccacc atacgatctt ttgagacgcg aagagaaagt accttagcat
    12361 gctgttttcc gcgaaggcaa ggcgcgaacc ccgcctctca tagcgtcgaa gcgggatcga
    12421 tggtatcctg atccggtcgg gacaactcgc tcgacaacca cactcagtca gacgctccgc
    12481 ggtggtgagg cgatgcccca aaaccggttc gacagcggcc cccgcttcat cccccacacg
    12541 tccccgcggg ctcgatcgat ccaccttttt atcggcctgc tctctatcgc cctgtccctg
    12601 ctcctcacct gcccgagtcc aagccaagct gaggttcgag tcgtcaccgg aacgggcgaa
    12661 catcgaatga acgatcacga aaccaaaacg gatgggatca gactcgccac cgagcaggct
    12721 aagaagcagg cgttggagca ggtcgcctcg tatctggaaa gcgtcacggt cgtgcgagat
    12781 ctggatgtca ctctggatga aatccgcagt tacaccgcgg gaatggtgct ggtcctcgat
    12841 caacacgtga cgaccgccct gaatggagag ttcgttgtgg tccgcgtcga gctgacggca
    12901 caggtagaca ccgacgaagt cgcccaggcc attgccaccc tacggcagaa cgaggacgcg
    12961 cgggagcagt tgcttgcgct cagacgggat gtggaatcgt tgcagcagga actcgacgca
    13021 gccaacgaca ccctcgccgc agccacctcg cccgaacagg tccaagaact gagccacgaa
    13081 cggaacgagt tgctcaatcg cgcggagtcc gatgcgatgg tcgcccaagc ctggaccgac
    13141 tgggtgctac tcactccgtt cgcacaaccc tctccatcgg ccggcctcgc gcaaattcac
    13201 gccctccttg ggctagcgag tcggctgaat cccgacaatc cccacctggc catcgcgcag
    13261 cagaccgtgg gaacgaaagc acccccggcg ccgccgcaac cccggcggcc gcccgtgcca
    13321 cataccgttc catttttgcc gggctacgcg gtcgtcccgc aacaaccggc tcaaccaggc
    13381 gcaattcaag taccggcaca gcctggagca ccgctgccgc aggggagcct cggcacgatg
    13441 ccgacctatc gacgagtccc gccaccatcc ggccgaccgg cagcgcgcac gctccatacc
    13501 tatcgatcgg gagtcgacca ggccactcct gctgcggaac aaccatcgat caatgcacct
    13561 cgcggaaccc cgccggcgat tatctaccag gtaccgaaga aaccggtgcc catcccgacg
    13621 acgccttcgc aacagatccc cggaaacgga actcatcagg gaggaacgcg gtaatgtcgc
    13681 tgacggctgg acccattaga acaggatggt ggccgctctg cgcgctgctg atcgcatcgc
    13741 cggcaaccgc tgcagacctg acgccaccgg ccgtgcgcga tcacgctgac cagaccttcc
    13801 aacatctcca aatgcaagga cgggcggaat cgccgaccag cgcagcccag gctagacctg
    13861 cgcaagcgac atccagctca gaggagttgc tgaccggcca ggggcagggc gacctgggca
    13921 aagggaaact cgtctgtcag cgggtctctg aactggcagc cagggccgac attgccaaac
    13981 aaatccgcgt gctggtgaag gaacatgcca ccgaccgcct gcgggaacgc accggtcgcg
    14041 aggtcgagca ggacatcgaa gtcgtgcggg aggaaatcgt tcaggaatat ttgcagggtg
    14101 tccgcatcgt cgaacgccac accgatgaag aagccaagac ctgttcagca acagccgtca
    14161 tgccgaagcc ccgcctgtcc acacccgcgt ccacggaatc gaacggccgc gagccggcag
    14221 cgcatcgctg aaccacctga tcaaatccac agctcctcca ttgaacttcg cgctccgttt
    14281 cgggtaagac aatcttccca tgccgatcga aaacaatttt cagcacgacg agctctcccg
    14341 caaaaatccc ggcgaccggc tgacctacgc ggactggacg acgccgcccg acgccgactc
    14401 tccggcctgg caggaggcga tgagccagct cggccagaag ctgtcacaag ccggtgtgcg
    14461 gctgatcatg ttcctgcatg gcgccatccc cggcaccgat gtcttcggct taggacggct
    14521 cgatgaagtg ggcgggctca agcgaggcta ttcacgcggc atctcgggcc tggattcgct
    14581 cctctccttc atgcgggaag gcacgaatgg catcaccccg ctgccgggcg gcatgaagcc
    14641 accgctgtcc aacgatgagg ccacgaagac cctgctcgac agtcagatcg gcgacgcggg
    14701 aaactttacg accaccacgg tcgaacattg ccggaaagcc ctgaatcaga aactggctac
    14761 acccatcacc tgcatccggg agctctggtc cagcgaacac catcacctcg gccgcgccat
    14821 ggccgcctgc cggttattgg accggctgcg ggccctcgtc attgaacaac atctaggcgc
    14881 cggcgaccgc atcctgattc aagcccatgg ccaagccggg ctcgtccttg cgctggcctc
    14941 taatctcctc tgcccttcgc cgatcacggg acggaagacc ctcttcgatc ttctcctcgc
    15001 cgcgaatcca caaggtccgg atgcgcaggc cattcagcgc atcgacccgt tgttgaccaa
    15061 tggcaccttg ctcaatggcg tagccctcga tatcgtcaca ttcggcactc cggtgcggta
    15121 cggctgggat ccgtccggca tcggcaaact actccacgtc gtaaaccatc gcaatctgcg
    15181 caccgacgga aaaacctggc tctccaaaat ggatctgcca cagatcaccg ttgaaatgcc
    15241 gatcgcctgg ggcggcgact acgtgcagga actggccgtg gccggcagcg atgccctgcc
    15301 cgctgaaaat gccaaagccg ccaataaggc tgtgtgggaa ttgctggaac cctacgatgg
    15361 attcgaacgc tgggtggagt gcgccagacg cgccggacgc ttcccgagcg aaggtcagtg
    15421 tctgctggtc gattacaaag actgcaccag ctcaaccaac gtccgcgacc actactacgg
    15481 ccacgccgcc tacacccgca ccaacgcgat gctcttcaac ttcaatcaga tcgttgggcg
    15541 g
//
LOCUS       DAOQRM010000052        15343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2934_length_15343_cov_17.981554, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000052 DAOQRM010000000
VERSION     DAOQRM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15343
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2934_length_15343_cov_17.981554"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1038)
                     /locus_tag="PKK81_06135"
     CDS             complement(1..1038)
                     /locus_tag="PKK81_06135"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32485.1"
                     /translation="MSTHQDRIETALLADDWDRVGYEASEWARALETAGEKDPRPYFA
                     LNVTHLIRGEFADAWRVYARALQETDDIERVREWVHSIADRHAGNAQTHLVQGLFLAQ
                     SGQSEQSMASYKEAAKLAPQSPYPHYFLAQIHERAAHLEMAIKEYREAVKLAPTFAPA
                     RTNLGVAYQEQGRLEMAIPQYREVIKLNPHDALAHANLGCALAEQGKFEPALQAYKEA
                     LRLNPSDAEIHFALGGVYETKGRTDLALKEYREATQLNPELASAHTALGWLLMEQSRA
                     SEAMEAFSKAVKANPEEAQALYGIGRIYAAKGKRESAAENFSKAIRFEKDPTKKNAIM
                     NALFASGQTGD"
     gene            complement(1058..>2359)
                     /locus_tag="PKK81_06140"
     CDS             complement(1058..>2359)
                     /locus_tag="PKK81_06140"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNV32486.1"
                     /translation="IADLGHVIICFEQISELAAIRLEDEPLWDGQAKSSRYQNFASTS
                     WFVPDTIRGQETEATYLGILRGLATVYRALHEPLSQHLTEREPRPEDMTPAAYQRAIA
                     ARAFDVTRYLLPLAAQTNVGQVVSIRTLEKQITRLLSSQLPELRLLGEELQEACRKPP
                     VNLWGELSGQAAGLGEPMAPTLARYAKPNLYQAEVYSDLARYAKEALKGTGLDQPTAW
                     GAAEPVDLIEPHHPLDEVVTTLLYRASQAPYRKILAVVRDWTEKQKQDTIEIAFQKRG
                     PHDELIKEFRSGYAFVFDVMMDIGGWRDMHRHRRCQQVQQNFTTVHGFETPPILAEAG
                     LDHEYREAMGHVKSDIEQLRKSSQEAAMYAIPFGFSVRCLFKMDYAEAEYIAKLRSGV
                     KGHWSYRTIAWLMKQKLTERYPILGARMQATPPDVQDALTR"
     assembly_gap    2360..2459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2460..3452
                     /gene="hisS"
                     /locus_tag="PKK81_06145"
     CDS             <2460..3452
                     /gene="hisS"
                     /locus_tag="PKK81_06145"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNV32487.1"
                     /translation="FIEHNRAADPRPQKYCYAGPMFRHERPQAGRLRQFHQFGVESFG
                     VSDPRADVEVMSLLWRLLSDLTLPGLTLEINNLGYAEDRARYKPLLVAFLNGVESRLC
                     GNCQRRIETNPLRVLDCKVPECRSATEDAPCLADSLSPAARDHFERVTTGLQSVGIPF
                     HMNTRLVRGLDYYCLTAFEVTCSHLGAQNAVGAGGRYDGLVEQLGGAAVPAVGFAVGL
                     ERIALMLPETVVIPAIPRVYVAAFGTQAVGVGFTLLDELRRTGVPADMDFRAASLKAH
                     LRQADRLNALYTILLGDDEVTKGVATLRNMQTKAQEDIPISDLATALRTRLVNR"
     gene            3574..3894
                     /locus_tag="PKK81_06150"
     CDS             3574..3894
                     /locus_tag="PKK81_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNV32488.1"
                     /translation="MSAKKFGILLSTPPSHPSVETVAQLASEALTEGADLYLYFIDEG
                     VKNLRDPRYTELVGRGIKLFVCAYGCQQHGVSTDALDSRISLCGLVVLSNIVNGCDRF
                     LAFT"
     gene            3918..4268
                     /locus_tag="PKK81_06155"
     CDS             3918..4268
                     /locus_tag="PKK81_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32489.1"
                     /translation="MAATTSIVLIIREDPRTSARPVEALRIALGLAAGENPITVILMG
                     QAVQLLAEETDDIVDIEILEKYLPSFEHLQIPFLLVFPSGPAPELQEGFAVTVGSSES
                     ARQAMAAADRVLVF"
     gene            4284..4556
                     /locus_tag="PKK81_06160"
     CDS             4284..4556
                     /locus_tag="PKK81_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32490.1"
                     /translation="MSARKTLYLLRRPISDPAQSLLPSASTDVSSGVCSLVLLEEAVG
                     SSPAFPGPIYVLPSASGSAATAASGKIISYRELVGLIAEHDSTIVL"
     assembly_gap    4563..4662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5068..6408
                     /gene="dnaA"
                     /locus_tag="PKK81_06165"
     CDS             5068..6408
                     /gene="dnaA"
                     /locus_tag="PKK81_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNV32491.1"
                     /translation="MWNDALVYIQEKVPKQVFETWFTPVVLDRIEDTTAYLAVPNKFF
                     GEWLGEHYRDLLAEAVSVAQGGGHLDVSFVVNHKQAPSSSPAQQESGPADTAGRGFVA
                     SRSKRGVQLNPKYTFKSFVVGAGNQFAHAACMAVAEQPAKAYNPLFLYGGVGLGKTHL
                     LNAIGNYLAERSDLRIAYLTTEQFTNEVINSIRYDKMIDLRKRYRNVDMLMIDDIQFL
                     AGKERTQEEFFHTFNTLYEAHKQIVLSSDRFPKDMPDIEERLRSRFEWGLIADLQPPD
                     VETRIAILRKKSEDERIALPEDVIHFLATTMKNNIRELEGSLVRVGAYSSLTGQTITL
                     DMAKNVLRDLIGDKKKIVSIEDIQEAVGSKYHLKIADLKSRRRSKTLVHPRQIAMYLC
                     RELTDASFPEIGRQFGGKDHTTIIHACRQITKAKEADSTLHTTLEGLKEQILRA"
     gene            6431..7561
                     /gene="dnaN"
                     /locus_tag="PKK81_06170"
     CDS             6431..7561
                     /gene="dnaN"
                     /locus_tag="PKK81_06170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNV32492.1"
                     /translation="MKVRIGREELLTGLQRVQGVVEKRNTMPILSNILLEAKHDGAEI
                     VATDLEIGMRGLYKATVLEAGGVTISARKLYEIIKELPSGEIELTSGDNNWTTIQSGK
                     SQFKVVGLPSGDYPALPSIEREGLTPLAGAGLLELIRKTLFAAGDNDARYILNGLLVS
                     LTTTEKKTTLLRLVGTDGHRLAVAEREVGTPNAKQPAQDIKAIIPKKAAQEMRRLLEE
                     GGDEEPLIGFTKNLMIFRKSGLLLTSRLMEGNYPNYQQVVPKESGKRIVVNRGLLESA
                     LRRVSVLSKDKANAVKVSFAPGGMTLFSSNPDYGEATEELVARYEGEALNTGFNARYL
                     LDALSVMDGESVSLQMDTALSPCLIQEAESPGFKCVVMPIKI"
     gene            7715..10189
                     /gene="gyrB"
                     /locus_tag="PKK81_06175"
     CDS             7715..10189
                     /gene="gyrB"
                     /locus_tag="PKK81_06175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNV32493.1"
                     /translation="MAKDDQQDNSAKPKSDSYSADQIKVLEGLDAVRKRPAMYIGSTG
                     VDGLHHLVYEVVDNSVDEHMAGFGEAIEVTIHIDGSVTVVDNGRGIPTGMHSTQKKSA
                     AEVALTVLHAGGKFEQGAYTVSGGLHGVGISVVNALSEWLELEIWQDGQVFEQRYERG
                     KPQAPLAVTGKTKRRGTKVRFMPDGQIFETLEFSFDVLAQRLRELAFLNKGLSITLKD
                     DRKEKEQVFHYKGGIVSFVDHLNEAKTPLHKPIYVQTERADLILEVALQYNDGYAENL
                     FSFANNINTKEGGTHLVGFKAALTRTINSYANANDLLKKDTESLSGDDVREGLTAVVS
                     VKVRNPQFEGQTKAKLGNSEVKGIVEAAVNDALGTYFEENPPVARKIIGKAIDAARAR
                     EAARKAKDLIRRKSALDGGSLPGKLADCSEKDPALSELFIVEGDSAGGSAKQGRDRKF
                     QAILPLKGKILNVEKARFDKMLSSDEIRTLILALGTGIGRKKEDSDKPDKEAFDIART
                     RYHKIVLMTDADVDGSHIRTLLLTFFFRQMPELLERGYIYIAQPPLFKVKKGKTERYL
                     KDEPAMNEYLADLAVEEVEVALENGREYLSGRRLLPTLKKLIAFESLLHKVNKKHHEA
                     NMLRIFVDEPGLTREALKDQAALTTIVANAKATLTLVYPKAEPTVDILEDEEHQSSKL
                     VCKVATGGIAYQLDVTHELVGSADFRELQKQAPSAMGLGKPPYKIKIKGTEVHKNGSA
                     ELVQAILEEGKQGLNIQRYKGLGEMNPGQLWETTMDPEKRTLLRVKLEDMTGVDEIFT
                     ILMGDEVEPRRNFIQQHALEVRNLDV"
     gene            10367..12829
                     /gene="gyrA"
                     /locus_tag="PKK81_06180"
     CDS             10367..12829
                     /gene="gyrA"
                     /locus_tag="PKK81_06180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNV32494.1"
                     /translation="MPPDERLGQIAIEDEMRSSYLDYAMSVIVGRALPDVRDGLKPVH
                     RRILHGMNEMGLAANRPYRKSAKIVGEIMGNYHPHGDSAIYDTLVRMAQNFNMRYMLV
                     DGQGNYGSMDGDAAAAMRYTEARLTKLAEELLADIEKETVDFGPNYDESRVEPLVLPS
                     RVPNLLVNGAGGIAVGYATNIPTHNLGEVIEGLLLLLENPDVTIPQLMKKIPGPDFPT
                     AGFIYGTSGIKDAYETGRGLLTVRAKVAIETDERTDRERLIVTEIPYQVNKSKLIEKI
                     ADLAQEDRVTGISDIRDESDREGVRVVIELKRNEIPLVVLNNLYKHSQLQTTFGVNML
                     ALVNNRPEVLNLKRILEAFVEHRREVVVRRTAYDLRKAEERAHILEGLKIALDNLDAV
                     IALIRRSQSPDVARTGLMQQFRLSEIQANAILEMRLQRLTQLERDKLVEEYREVLKTI
                     EYLRSVLGSEALVRKIIQDELIEIKDKYQDERRTKIVKEEAELTLEDLIAEEEVVVTI
                     SHAGYIKRNAVTLYRAQRRGGKGKIAMGIKEEDFVETLFTASTHDSLLFFTDAGKVYW
                     LKVHEIPEASRAAKGKALVNLLALSKDEKVTASLPVKEFRADRFIIMGTKLGVIKKTE
                     LSAYSNPRQGGIIALSLDAGDKLIGVDLTDGQREILLGTKQGITIRFKEEDVRAMGRT
                     AHGVRGITLEPGDEVIGMETITPDSTTAILTVTEGGYGKRTPVNEYRVQGRGGKGIIS
                     VKTTERNGPAVGFLQVRDEDEIMLMAAQGKVLRCKVDDIREIGRNTQGVRLLDMDGDE
                     DRVVAVVRLVEREEGVPEEGES"
     gene            12876..13490
                     /locus_tag="PKK81_06185"
     CDS             12876..13490
                     /locus_tag="PKK81_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32495.1"
                     /translation="MNHPTPPSAGAPTRTPAKRPLDTVVKMALGVLFGSFALIWGGMF
                     LSRPDRSIPPYSIGSQERTVVAVHVPSWTSDTEIETLIERFRKVGAESRNFGSMKIQP
                     TTLDDPKGRYQHIAIYIFTDEGWTEPEVLHKYVTGDDATVRDGFEGSVRGLYRLDEQE
                     EEGRIGPVRRGKDSAATAAYSRQLFKGTVVRNAAATPADSTPAP"
     gene            complement(13429..13935)
                     /locus_tag="PKK81_06190"
     CDS             complement(13429..13935)
                     /locus_tag="PKK81_06190"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNV32496.1"
                     /translation="MRGQSRLDSFSSILAGVAHRLGLESKMFEARLRRQWPDIVGEPI
                     AAHTRPDQIRFKKLYVLVHNSVWLQQLTFLKPVLLEKVNAMAGQPLVTEIVLRIGEFT
                     ADHSSTAEATTDPEPLAIQPSPQLLQEITLHAQGIQDQALREHLVTLMAQALSQPESP
                     PRSARRSP"
     gene            14180..14659
                     /gene="smpB"
                     /locus_tag="PKK81_06195"
     CDS             14180..14659
                     /gene="smpB"
                     /locus_tag="PKK81_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNV32497.1"
                     /translation="MTKTHDKEDQYKVVATNRKAYHDYFIEEKFEAGVILRGTEVKSL
                     REGRVNLQDSYASVDDGEVYLQHCHISPYSHGNLMNHDPLRKRKLLLHRKEINKLIGK
                     TQLKGLTLVPLRIYFSKRGHAKVELALAKGKKQYDRRESIKAREAGREVERAIKQRK"
     gene            14986..>15343
                     /locus_tag="PKK81_06200"
     CDS             14986..>15343
                     /locus_tag="PKK81_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNV32498.1"
                     /translation="MKALFNTDDGWSGLILRLTLGLVMLPHGAQKLLGWFGGFGFDGT
                     MGFFTQKMGLPWIIAFLVIMGEFFGSLGLLAGLLTRFTAASFIVIMLGAILTVHLPVG
                     FFMNWFGQQQGEGYEYH"
BASE COUNT         3226 a         4261 c         4615 g         3041 t
ORIGIN      
        1 tcaatcccct gtctggccgc tggcgaagag ggcgttcatg atggcgtttt ttttggtggg
       61 atctttttcg aagcggatgg ctttggagaa gttttcggcg gcactttccc gcttgccctt
      121 ggcggcgtaa atcctcccga tgccgtagag ggcctgggcc tcctccgggt tggccttcac
      181 ggctttgctg aaggcctcca tggcctccga tgctctcgat tgctccatca acagccaacc
      241 aagcgcggta tgggccgagg cgagttccgg attgagctgc gtcgcttcgc gatattcctt
      301 gagggcaaga tcggtccgcc ccttggtctc gtaaactccg ccgagcgcga aatggatttc
      361 cgcgtcgctc gggttgagcc gtagggcttc tttgtaggct tgcagcgccg gttcgaattt
      421 gccctgctcc gcaagcgcac agccgagatt ggcatgggcc agcgcgtcgt ggggattcag
      481 cttgatcact tcgcggtatt ggggaatggc catttcaagc cggccctgct cctggtaggc
      541 cacgccgagg ttcgtgcgcg ccggcgcaaa tgtcggagcg agcttgaccg cctcgcgata
      601 ttccttgatc gccatctcca aatgcgccgc ccgctcgtga atctgggcca gaaaataatg
      661 gggatagggc gattgcggag ccagcttcgc ggcctctttg taggacgcca tcgactgttc
      721 cgactggccg gactgcgcca gaaacaatcc ctgcaccaga tgcgtctgag cattgccggc
      781 gtgccgatcc gcgatggagt gcacccactc ccggacgcgc tcgatgtcgt ccgtctcctg
      841 cagcgcgcgg gcatacaccc gccaggcgtc ggcgaattcc ccgcgaatca ggtgggtgac
      901 gttcaaggcg aagtacgggc gaggatcttt ttcaccggcg gtctccagcg cgcgggccca
      961 ttccgacgcc tcatacccga cccggtccca gtcgtctgcc aggagggccg tttcgattct
     1021 gtcttggtgc gtgctcatga tgaaagaggc gcgccgctca gcgcgtcaga gcgtcctgca
     1081 catccggcgg agtcgcctgc atgcgcgcgc cgagaatggg atatcgttcg gtcagtttct
     1141 gtttcatgag ccaggcgatg gtgcgatagg accagtgccc cttcaccccg gaacgcaatt
     1201 tcgcaatgta ttccgcttcc gcatagtcca tcttgaagag gcagcgcacg gaaaatccga
     1261 acgggatggc atacatggcc gcttcctggc tcgatttccg gagttgttcg atatcggatt
     1321 ttacatgccc catggcttcg cggtactcgt ggtcgagccc cgcttccgcc aaaatgggcg
     1381 gtgtctcgaa cccgtgcaca gtggtgaagt tctgctgcac ctgctggcag cgccggtgcc
     1441 ggtgcatgtc gcgccacccg ccgatgtcca tcatcacatc gaacacaaag gcatacccgc
     1501 tgcggaattc cttgatcaat tcgtcatgcg gtccgcgttt ctggaaggcg atttcgatgg
     1561 tgtcctgctt ctgtttctcc gtccaatccc gcacgaccgc gaggatcttt cgatacggag
     1621 cctgcgaggc gcgatagagc agcgtggtga cgacttcgtc gagcggatgg tgcggctcga
     1681 tcaagtccac cggttccgcg gcgccccagg cggtgggttg atccaacccg gtgcccttca
     1741 atgcttcttt cgcataccga gcgagatcgc tgtagacctc tgcctgatac agattcggtt
     1801 tggcgtaccg cgcgagggtc ggcgccatgg gttcgcccaa gccggccgcc tgtccggaca
     1861 gttcgcccca gagattgacc ggcggtttcc gacaggcttc ctgcagctct tctcccagca
     1921 gacgaagctc cggcagttga gacgacaaca ggcgggtaat ctgcttttcg agagtgcgaa
     1981 tgctgacgac ctggccgacg ttcgtctgcg ccgccagggg caacagataa cgggtcacat
     2041 cgaaggcccg cgctgcaatc gcccgttgat aggcggcggg ggtcatgtcc tcggggcgag
     2101 gctcccgctc cgtcagatgc tgactcaacg gttcgtggag cgcacggtag accgttgcga
     2161 gacctcggag aatacccaga taggtcgctt ccgtctcctg cccgcgaatg gtatccggca
     2221 cgaaccaact ggtcgatgcg aagttctggt accggctcga cttggcctgc ccgtcccaga
     2281 gcggttcgtc ctccaggcgg atggcggcca gctcggagat ctgttcgaag caaatgatca
     2341 catggcccag gtccgcgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2461 tcatcgagca caaccgggcc gccgatccgc ggccgcaaaa atattgttac gccgggccga
     2521 tgttccgcca cgagcgtccc caagccgggc gactgcggca gtttcatcag tttggtgtgg
     2581 aatccttcgg tgtgtccgac ccgcgggccg atgtcgaagt catgtcgctg ctgtggcgac
     2641 tcttgtccga cctcacgctg ccgggattga ccctggaaat caacaatcta gggtatgcgg
     2701 aggaccgggc ccggtacaaa ccgctcctgg tagcgtttct gaacggtgtg gaaagtcgcc
     2761 tctgcgggaa ttgccagcgc cggatcgaga cgaatccgtt acgtgttctc gactgtaaag
     2821 ttccggaatg ccgatcggct accgaggatg ctccctgcct ggccgattcc ttgtcacccg
     2881 cagcgcggga ccatttcgag cgcgtgacga ccggcctgca atcggtgggt atcccgtttc
     2941 acatgaacac tcggctcgtc cgtggcctgg actattactg tctcaccgca ttcgaggtta
     3001 cctgctccca tttgggtgcg cagaatgccg tcggtgccgg tggacgctat gacggtctcg
     3061 tcgaacagct tggtggagcc gccgtgccgg cggtcggatt cgccgtcggg ctggaacgga
     3121 ttgcactcat gttgcctgaa acggtggtga taccggccat tcctcgtgtc tatgtcgctg
     3181 cctttggcac tcaggctgtc ggtgtcggat ttacgctgct cgatgaactc cgtcgaaccg
     3241 gagtgccggc tgacatggat ttccgtgcgg cgtcactcaa ggcccacttg cgtcaggccg
     3301 atcgcctcaa tgctctctat acgatcctcc ttggtgacga tgaagtgacg aaaggtgtcg
     3361 ccaccctcag gaatatgcag acaaaagccc aggaagatat cccgatctca gacctcgcca
     3421 ccgccctccg taccaggctc gtcaacaggt aattcagagc cctttttcta gttgactttc
     3481 tctccaagaa ttcgtacgct ccgcattcta ttttatatat ataagttatt gattcttaat
     3541 cactcttaaa gattatccac agacaaaatt cctatgagcg ccaagaagtt cggcatcctg
     3601 ttatccacac ccccctcaca ccccagcgtc gagacggtgg ctcagctcgc ttctgaggcg
     3661 ctgaccgaag gtgctgatct ctatctatat ttcatcgatg agggagtgaa gaatcttcgt
     3721 gaccctcgtt ataccgaact cgttggccga ggtataaaat tgtttgtctg cgcctatgga
     3781 tgccagcagc atggagtctc gaccgatgct ctcgattccc gcatttctct ctgcggcctg
     3841 gtcgtgctgt cgaatattgt gaacggctgc gaccggtttc tggcatttac ataagacgat
     3901 tgtctgaagg aacgactgtg gcggccacta catccatcgt gctgattatt cgtgaagacc
     3961 cgcgaacgtc tgctcgtccg gtcgaggcct tgcgcatcgc attgggattg gctgccggag
     4021 agaacccgat tacggtgatt ctcatgggac aggccgttca gctgctggcg gaagagacgg
     4081 atgatatcgt cgatatcgag attctcgaaa agtatcttcc ctccttcgag catctccaga
     4141 ttccgtttct cctggtgttt ccatccggcc cggctcccga gcttcaagag ggattcgcag
     4201 tcactgtcgg ttcttctgaa tcggcccggc aggccatggc cgcagccgac cgggtattgg
     4261 tcttttaact tctatggaat ctcatgtctg cccgtaaaac gttgtatcta ctccgacgac
     4321 cgatttctga tccggctcaa tccctgttgc cttccgcatc gacggatgtg tcgtcaggcg
     4381 tctgctcgtt ggtcctcctg gaagaggcgg tggggtcttc accggcgttc cccggaccga
     4441 tttacgtgct tccatctgca tcaggcagtg cggcaaccgc cgcttccgga aaaatcatct
     4501 cctatcgaga gctagtcggc ttgattgcgg agcatgattc aaccattgtg ctgtgatccg
     4561 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagagctag tcggcttgat
     4681 tgcggagcat gattcaacca ttgtgctgtg atccggccct cttctcttag ttatcttcct
     4741 aataaagaga agaaccggag ccgtcggagt aggagtaggc tatgtgggtt ctgtggatga
     4801 atgcgaagag gtccgtgggc gttgacgatc gaagagccta gcccatgttg atgagatggt
     4861 gtataaggca gggggcgttc agggataagg tgagggtacg tgtgcacgga aaagatgaac
     4921 agtcggccgg tctctcagat gtccacaaga agcgcgcgcg tcttctcatt gagcgatcaa
     4981 taaaaccagg gcttcctatg tatttatcca cacctgtgta taaggctgtg aacattcata
     5041 agatattgag tttataggac taattagatg tggaatgacg ccctggtgta cattcaggaa
     5101 aaggttccga agcaggtctt tgaaacctgg ttcactcctg ttgtactcga ccggattgaa
     5161 gatacgacgg cgtatcttgc agtcccgaac aagtttttcg gcgagtggtt aggagagcat
     5221 taccgcgatc tcctggcgga agcggtttcc gttgcgcaag ggggcggcca cttggacgtg
     5281 tcctttgtcg tcaatcacaa gcaggctcct tcctcttcgc ccgctcaaca ggagagtggg
     5341 cctgcggata cggccgggcg tggattcgtt gcgtctcgat caaaacgcgg cgtgcaattg
     5401 aatcccaaat ataccttcaa gagtttcgtg gtcggggcag gaaaccagtt cgcccacgcg
     5461 gcctgtatgg ccgtggccga acaaccggcc aaagcctaca accccctgtt tctctatggc
     5521 ggtgtgggat tgggcaagac ccacttgctg aatgcgatcg gcaactacct ggccgagcgg
     5581 agcgacctcc gcatcgccta cctgacgacc gagcagttta cgaacgaagt catcaattct
     5641 attcgttatg acaagatgat cgacctgcgt aagcggtatc gcaacgtcga tatgttgatg
     5701 atcgacgaca ttcaatttct ggcgggcaaa gagcggacgc aggaagagtt ctttcatacg
     5761 ttcaataccc tctacgaagc ccataaacag atcgtcctgt cgagcgaccg ctttccgaag
     5821 gacatgccgg atatcgaaga gcggctccgt tcccggttcg aatgggggct cattgccgac
     5881 ctgcagccgc cggacgtgga gacccgcatc gccatcttgc gcaagaaatc cgaggacgaa
     5941 cgcattgcgc tgcccgagga tgtgattcac ttcctggcca ccacgatgaa gaacaacatt
     6001 cgtgagctgg aaggctcgct cgtgcgggtc ggggcctatt cctcgctgac ggggcaaacg
     6061 atcacgctcg acatggctaa gaacgtcttg cgcgatctca tcggggacaa aaagaagatt
     6121 gtctccatcg aagatattca agaggcagtg gggtcgaagt atcatttgaa gattgccgat
     6181 ttgaaatcgc ggcgtcggag caaaacgttg gtgcacccgc gccagatcgc catgtatctt
     6241 tgccgggaac tcaccgacgc ctcatttcct gaaatcggcc gccagttcgg gggtaaggac
     6301 catacgacga ttattcacgc ctgccggcag atcacgaagg ccaaggaagc cgacagcacc
     6361 ctgcacacga cgctggaagg cctgaaagaa cagatcttga gggcgtaagg cccgctgagg
     6421 gagaccgacc atgaaggtac gcatcggacg agaggaattg ttgacgggat tgcagcgggt
     6481 gcagggtgtc gttgaaaaac gaaacaccat gcccatcctc tcgaacattt tactggaagc
     6541 gaagcacgat ggcgctgaaa ttgtcgctac cgatctcgag atcggcatgc ggggtctcta
     6601 caaagcgacg gtcctcgaag cgggcggcgt gacgatttcc gctcgtaagt tgtacgagat
     6661 catcaaggaa ttgccgagcg gcgagatcga gctgacctcc ggcgataaca actggacgac
     6721 gatccagtcc gggaagagcc agttcaaggt ggtcggcctt ccgagcggcg attatccggc
     6781 gttgccctcc atcgaacgcg aggggctgac gccgctggcc ggggcgggtc tgttggaatt
     6841 gattcgcaag acgctgtttg ccgcgggcga caatgatgcg cgatacatcc tgaacggcct
     6901 gctcgtgagc ttgacgacaa ctgaaaagaa aaccacgctc ctgcgactgg tcggcaccga
     6961 cggccatcgc ctggccgtag cggagcgcga agttggtacc ccgaatgcga agcagccggc
     7021 ccaggatatc aaagcgatca tcccgaagaa ggcggcgcag gaaatgcggc ggctgctgga
     7081 agaggggggc gacgaagagc ccttgatcgg gttcaccaag aatctcatga tcttccgcaa
     7141 gagcggcctc ctcctcacct cccgcctcat ggaggggaat taccccaatt accagcaggt
     7201 ggtcccgaaa gagagcggca agcgcattgt ggtcaatcgc gggctgctgg agagcgcgtt
     7261 gcgacgggtc tcggtgttgt cgaaagacaa ggccaatgcc gtgaaggtgt cctttgctcc
     7321 cggcggcatg actctgtttt cgagcaatcc ggattatgga gaagccacgg aagaactggt
     7381 ggcccgatat gagggcgagg cgctcaatac aggattcaat gcccgttacc ttttggatgc
     7441 attgagcgtc atggatggag aatcggtctc gttgcaaatg gacacggcct tgagcccctg
     7501 cctgattcag gaagccgaga gtcccggatt caaatgcgtc gtgatgccga tcaaaattta
     7561 gcaataggaa tgccttttta caggatgctc aacgtggctg tccggcaagg ccgcagtgag
     7621 tggagggccg aggcgtaccc ttgcggtacg tggagggcct gcacgatgcg agcacgccgc
     7681 cggcggacat tgtcagcatc ctgctaggag ctgaatggcc aaggacgacc agcaggacaa
     7741 ttctgccaag ccgaaatccg acagttacag cgcggatcaa atcaaagttc tcgaaggact
     7801 cgacgccgtc cggaagcgac cggcgatgta catcggcagc accggtgtcg acgggttgca
     7861 ccatctcgtc tatgaagtcg tcgacaacag tgtcgacgag cacatggccg ggttcggtga
     7921 agcgatcgag gtcacgatcc acatcgacgg cagcgtcacg gtcgtggaca acgggcgcgg
     7981 cattcccacc ggcatgcatt ctacgcagaa gaagtccgcc gccgaagtgg ctctcaccgt
     8041 cctgcacgcg ggcggcaagt tcgagcaggg cgcctacacg gtctccggcg ggttacacgg
     8101 agtcggcatc tccgtcgtca acgcgctgtc cgaatggctg gagctggaaa tctggcagga
     8161 cggacaagtc ttcgagcagc ggtatgagcg tggaaaaccc caggcgccgt tagccgtcac
     8221 cggaaaaacc aaacgccggg gcacgaaggt gcggttcatg ccggacggcc agattttcga
     8281 aacgctggaa ttcagtttcg atgtgttggc gcagcggctc cgcgagctgg cctttctcaa
     8341 caaggggctc tcgatcacgc tcaaggacga tcgcaaggaa aaggaacagg tttttcatta
     8401 caagggcggg atcgtctcct tcgtcgatca cctcaacgaa gccaagaccc cgctgcataa
     8461 gcccatctac gtgcagactg agcgcgcgga cctgattctt gaggtggcgc tgcaatacaa
     8521 cgacgggtat gccgagaacc tcttttcgtt cgcgaacaac atcaacacta aagagggcgg
     8581 cacccacctg gtgggcttca aggctgcgct gacccgcacg atcaacagtt acgccaacgc
     8641 caacgatctc ctgaagaaag acaccgaatc gttgagcggc gacgacgtgc gggaaggctt
     8701 gaccgcggtt gtcagcgtga aggtgcggaa tccgcagttc gaagggcaga ccaaggcgaa
     8761 gctcggcaac agcgaggtga agggcatcgt cgaggcggct gtgaacgacg ccctcggcac
     8821 ctacttcgaa gagaacccgc cggtcgcccg caaaatcatc ggcaaggcga tcgatgccgc
     8881 gcgtgcccgc gaggcggccc ggaaagccaa ggacctgatt cgccggaaga gcgccttgga
     8941 tggcgggtcg ttgcccggca agctggccga ctgttcggag aaggatccgg cgttgagcga
     9001 acttttcatc gtcgagggtg attccgccgg cgggtctgcc aagcagggcc gcgaccggaa
     9061 gtttcaggcg attcttcccc tcaaaggaaa aatcctcaac gtcgagaaag cccgcttcga
     9121 caagatgctc agcagcgacg aaatccgcac gttgatcctg gcgctcggta ccggaatcgg
     9181 ccgcaagaaa gaggattccg acaagccgga caaggaagcc ttcgacattg cacggacacg
     9241 ctatcacaag atcgtgctca tgaccgacgc cgacgtggac ggcagccaca tccgcacgct
     9301 cctgctgacc tttttcttcc gccagatgcc ggagctgctg gagcggggct acatctacat
     9361 cgcccagcct cccctcttta aggtcaagaa gggcaagacg gagcggtatc tcaaggatga
     9421 gcctgcgatg aacgaatacc tcgcggactt ggcggttgaa gaagtcgaag tggcgctcga
     9481 gaacgggcgg gagtacctgt cgggccgccg cctgctgccg acgctcaaga agttgatcgc
     9541 cttcgaaagc ctgctgcaca aggtgaataa gaaacatcac gaagccaata tgttgcggat
     9601 atttgtcgac gagccggggt taacgcgcga ggcgttgaag gatcaggccg cgttgaccac
     9661 gatcgtcgcc aatgcgaagg ccacgctgac gctcgtctac ccgaaggccg aaccgaccgt
     9721 cgacatcctg gaagacgaag aacatcaatc cagcaagttg gtatgcaagg tcgccacggg
     9781 cgggatcgcg tatcaactcg acgtgacgca tgagctggtc gggtccgccg acttccgcga
     9841 gctccagaaa caggcgccct cggctatggg cctcggcaag ccgccgtaca agatcaagat
     9901 taagggaacc gaagtccata agaacggctc ggccgagctg gtgcaagcga tcctggaaga
     9961 aggcaagcag gggttgaaca tccagcggta caaaggtctt ggtgagatga atcccggcca
    10021 gttgtgggaa accaccatgg atcctgaaaa gcggacgctg ctgcgggtca agctggaaga
    10081 catgaccggc gtggatgaga ttttcacgat cctgatggga gacgaagtgg aaccccgccg
    10141 caatttcatt cagcaacatg cactggaagt caggaacttg gacgtatagc tggtcctgat
    10201 tgcgaagacg acaacttggt tttgtgaatt cggaggatgc tcaaaatggc gcttcggcaa
    10261 ggccgcaggc gagagaggac cggaggcgta ccctcagggg tacgttgagg accttcttga
    10321 gccgagaacg aagccgaggg acattttcag catccgtgga gggtagatgc cgccagatga
    10381 acgactagga cagattgcga tcgaagacga gatgcgctcg tcgtacctcg attacgccat
    10441 gagcgtgatc gtcggtcgcg cgctccccga cgtgcgggac ggattgaagc cggtgcaccg
    10501 gcgcattctg cacggcatga acgaaatggg tctggccgcg aaccggccgt acagaaagtc
    10561 ggccaagatc gtgggcgaga tcatgggtaa ctaccacccc catggcgatt ccgcgattta
    10621 cgacaccctt gtgcgcatgg cgcagaactt caatatgcgc tacatgctcg tcgacggaca
    10681 gggcaactac ggctcgatgg acggcgacgc cgccgccgcc atgcggtaca ccgaagcccg
    10741 gttgaccaag ctggccgaag agctgttggc ggatatcgaa aaagagacgg tcgatttcgg
    10801 ccccaactac gatgaatccc gtgtcgagcc gttggtcctg ccgtcccgag tgccgaatct
    10861 gctggtcaac ggcgccggtg gaatcgccgt cgggtatgcg accaacattc ccacgcacaa
    10921 tctgggtgaa gtgatcgagg gactgctcct gttgctggag aaccccgacg tcaccatccc
    10981 gcagctgatg aagaagattc ccgggcccga cttcccgacc gccgggttca tttacggcac
    11041 ctccgggatc aaggatgcgt acgagacggg acgcgggctg ttgacggttc gcgccaaggt
    11101 cgccatcgag acggacgagc gcaccgaccg tgaacggctc atcgtgaccg aaattcccta
    11161 ccaggtgaac aagtcgaaac tcatcgaaaa gatcgccgac ctggcgcagg aggatcgcgt
    11221 cacgggcatc tccgatatcc gcgacgagtc ggaccgcgag ggtgtgcgtg tcgtgatcga
    11281 gctgaagcgc aacgagattc cgctcgtggt gttgaacaac ctgtataaac acagccagtt
    11341 gcagaccacc ttcggcgtca acatgctggc gctcgtcaac aaccggccgg aggtgctgaa
    11401 tctcaagcgc atcctggaag cctttgtcga gcaccggcgc gaagtggtgg tgcggcgaac
    11461 ggcctatgac ttgcgtaagg cggaagaacg cgcccatatc ctcgaaggtc tcaagatcgc
    11521 gctggataat ctggatgccg tgatcgcgct gatccgccgc tcgcaatcgc cggatgtggc
    11581 gcgcacgggt ttgatgcagc agttccggct gtcggaaatc caagccaacg ccattctcga
    11641 aatgcgcctc cagcggctca cgcaactgga gcgcgacaaa ctcgtggaag aataccgcga
    11701 agtgctgaag accatcgagt atctgcgctc ggtcctcggc agcgaggccc tggtgcggaa
    11761 gatcatccag gacgaactga tcgagatcaa ggacaagtat caggacgaac ggcgcaccaa
    11821 gatcgtcaag gaagaggccg agctgacgct cgaagatctc atcgccgaag aagaagtggt
    11881 cgtgacgatt tcccatgccg ggtatatcaa gcggaacgcc gtcacgctct atcgggcgca
    11941 gcggcgcggc ggcaagggca agatcgcgat gggcatcaag gaggaggatt tcgtcgaaac
    12001 gctctttacg gcctcgacgc atgactcatt gctcttcttc accgatgccg gcaaggtcta
    12061 ttggctcaaa gtgcacgaaa ttccggaagc cagccgggcc gcgaagggca aggcactcgt
    12121 gaacctcctg gccctctcga aggatgagaa ggtcacggct tcgctgccgg tgaaagagtt
    12181 ccgtgccgat cgctttatca tcatggggac gaagctgggc gtcatcaaga agacggaact
    12241 gtcggcctac agcaatccgc gccagggcgg catcattgcg ctctcgctcg atgcgggcga
    12301 caagctgatc ggtgtggacc tgaccgacgg ccagcgtgag attctgctgg gcacgaagca
    12361 gggcatcacc atccggttca aggaagaaga tgtgcgcgcg atgggacgta cggcccacgg
    12421 ggtgcgcgga atcacccttg agcccggcga tgaagtcatc ggcatggaga cgatcacgcc
    12481 cgattcgacc accgcgatcc tcaccgtgac cgaaggcggg tacggcaaac ggacaccggt
    12541 gaatgaatat cgcgtgcagg gccgcggcgg taaaggcatc atcagcgtca agacgaccga
    12601 gcgaaacggg ccggctgtcg gattcctcca ggtccgtgac gaggatgaga tcatgttgat
    12661 ggcggcgcag ggcaaagtgc tgcgctgcaa ggtggacgac atccgtgaaa tcgggcgcaa
    12721 cacccaaggt gtccgcctgc tcgatatgga tggagatgag gatcgtgtgg tcgccgtcgt
    12781 gagactggtc gaacgcgaag aaggagtgcc cgaagagggc gagtcatgac tccttggagc
    12841 cgcaccggtt tcaagcggct ccccccacca tggttatgaa ccatccaact ccgccatcgg
    12901 ccggggctcc gaccagaact ccggccaagc gtccgctgga caccgtggtc aaaatggcgc
    12961 tgggcgtcct gttcggctcc tttgctttga tctggggcgg gatgtttctc agccgtccgg
    13021 atcggtcgat tcccccctat agcatcggct cccaggagcg cacggtggta gcggtgcatg
    13081 tgccgtcctg gaccagcgac actgagatcg aaaccctgat cgagcgattc cggaaagtcg
    13141 gcgcggaaag ccggaacttc ggcagcatga aaatccagcc caccaccctg gatgatccca
    13201 aggggcgtta tcagcatatt gcgatttaca tcttcaccga tgagggctgg acggagccgg
    13261 aggtgctgca taagtacgtg acgggtgacg atgccacggt gcgggacggg tttgagggat
    13321 cggtacgcgg attgtaccgg ctcgatgaac aggaagagga aggccgcatc gggcccgttc
    13381 gcagagggaa agacagcgcg gccaccgcgg cctattcgcg gcagttgttt aaggggaccg
    13441 tcgtgcggaa cgcggcggcg actccggctg actcaacgcc tgcgccatga gcgtgaccag
    13501 gtgttcacgc agggcttggt cctgaatgcc ctgagcgtgg agcgtgattt cttggagtaa
    13561 ctggggcgac ggttgaattg caagcggttc ggggtcagta gtcgcttcgg cagtcgatga
    13621 atggtctgcg gtgaactctc cgatgcgcaa gacgatttcc gtcaccaatg gctggcccgc
    13681 catcgcattc accttctcca gcaggaccgg cttgaggaag gtgagttgct gtagccacac
    13741 cgagttgtgc accaggacgt agagtttctt gaaccggatc tgatcgggac gcgtgtgcgc
    13801 ggcgatcggt tcgcccacga tgtcgggcca ttggcgccgc aggcgcgctt cgaacatctt
    13861 gctctccaat cccagcctgt gggcgacacc ggctaagata gaactgaatg agtcgagtct
    13921 gctctgtcca cgcatggtgg tatgataaca aaatgtgccc gaaggaaata gctcgcgcga
    13981 gcccacagga tgcatgatgg ttcgtcgcga aagtgcggag tcgcgaagcg agacgggcgc
    14041 gtggagccgt aaggaaggga ggcatccttg aacaggatgt cgaccgaccg agcggcgagc
    14101 ccgcccgccg acaggcctgt cgcagccgcg aacccgcgat tgcagcagaa gcgctctgca
    14161 tcatgtgggc tagtatgaga tgacgaaaac gcacgacaaa gaagaccagt acaaagtcgt
    14221 cgccaccaat cgcaaggcgt atcacgacta cttcatcgaa gagaagttcg aagccggggt
    14281 catcctgagg gggactgagg tgaagtcgtt gcgggaaggt cgcgtgaacc tccaggatag
    14341 ttatgcctcg gtggatgacg gcgaggtcta cttgcagcat tgtcacatca gcccctactc
    14401 gcacggcaat ctgatgaatc acgacccgct ccgcaagcga aagctgctgt tgcatcgcaa
    14461 agaaatcaac aaactgatcg ggaaaaccca gctgaagggc ctcacgctcg tccccttgcg
    14521 catctacttt tcgaagcgcg gccacgccaa agtggagtta gccttggcga agggcaagaa
    14581 acaatacgat cgtcgcgaat cgatcaaagc ccgtgaagcc ggccgcgaag tcgagcgcgc
    14641 gatcaagcaa aggaaataag cagcttcgtc cctcacacct ctcttcctcc ctccctcctt
    14701 tcccgagaga gtccacgagc tctcggttgt tccccagagt tttcgatgca atcccccgtc
    14761 ccggcaggtc gttgagaggc ctgtgacccg tcgcgtatgt ccctataacc acaactgact
    14821 gatctgtaag aataggtgtc cttccggctg agggggaata tttgctcttg acttagttct
    14881 aattagaact attatactgg cgtcggcccg cgcggctgtt cgccgggcga tctgagcggt
    14941 gggcagagtc agacggttaa tggtcgatca cgggagggta cgaaaatgaa agcactattc
    15001 aatacagacg acggttggtc cggcctgatt ttgcggctga ccttggggtt ggtgatgttg
    15061 ccgcacgggg ctcaaaagtt gctgggttgg ttcggcggat tcgggttcga cgggaccatg
    15121 ggttttttca cccagaaaat ggggctaccc tggatcatcg cgtttctggt tatcatgggg
    15181 gagtttttcg gaagcctggg gttgctcgcc ggcctgttga cgcgattcac cgccgccagc
    15241 ttcattgtga tcatgctggg agccattttg acggtgcatc tgccggtcgg gtttttcatg
    15301 aattggttcg ggcaacagca gggagagggc tatgagtacc atc
//
LOCUS       DAOQRM010000053        15322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_2943_length_15322_cov_16.103098, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000053 DAOQRM010000000
VERSION     DAOQRM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15322
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2943_length_15322_cov_16.103098"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..886)
                     /gene="ricT"
                     /locus_tag="PKK81_06205"
     CDS             complement(143..886)
                     /gene="ricT"
                     /locus_tag="PKK81_06205"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNV32499.1"
                     /translation="MVEKLLAQELANPYPAVVRIVGVKIRDRGEVKKFDAGEASLVMG
                     DRVLLEVAGELSYGVVYGAPQVMPFIPPMRVLQPITRKATTEDVATIDRYERLASEGM
                     KACREKAAALGLRMKLVEVFCSFHRRQMTFVYTAEDRIDFRELVRLLARRFGGRIEMR
                     QVGVRDEASRLGGIDTCGLVLCCAAFLTEVKPVTVKQARVLGLPVDDPKLLGVCGRLK
                     CCLLFEAMDNPSSKSSALINPSRSRLASS"
     gene            1120..1368
                     /locus_tag="PKK81_06210"
     CDS             1120..1368
                     /locus_tag="PKK81_06210"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNV32500.1"
                     /translation="MVRIPNKVILPFGYHIMIRQVTDSEMDRQDSNADGIWDNEAKTI
                     YIRKRLPVTRRRYILAHELGHAWLDWQHRYLDDGKARS"
     gene            complement(1424..1909)
                     /locus_tag="PKK81_06215"
     CDS             complement(1424..1909)
                     /locus_tag="PKK81_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNV32501.1"
                     /translation="MADGRHYWLMKSEPKVFSIDDLAGSPKSTTSWDGVRNYQARNFM
                     RAMKIGDQVLFYHSNANPPAVVGVAEVVRAAYPDATQFDKRDSHYDPAGNPAQPRWDM
                     VDIRFVRKFLAPLSLDLLRAKAGLKGMELLRKGSRLSVQPVRAAEWNEIIRLEEVQAD
                     S"
     gene            1991..2803
                     /locus_tag="PKK81_06220"
     CDS             1991..2803
                     /locus_tag="PKK81_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNV32502.1"
                     /translation="MDLTPMWFGVYKALKYLLYPLSWILIAGLLALLTACLPVSPGRR
                     TWVRRLLFLTVLLLLLTATPVLSLLYIALLETQYPSFQPTSTSKFDAVVVLAGGVFQK
                     GSLRPADEVSDASRQRTGCGADLWQQNLAPKLLLTGGDATVFRTGLLESHEMKRWALR
                     LGVPESAILIEEQSRTTYENAVQTKAILGSGRIILVTAAYHLPRAVRLFEKQGFVVTP
                     FPCGYESQHTPQQAWEQATLFDFLPTAKALLITTQAVDEVAGIIVYWLAGKL"
     gene            complement(2897..3268)
                     /locus_tag="PKK81_06225"
     CDS             complement(2897..3268)
                     /locus_tag="PKK81_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32503.1"
                     /translation="MRLSGHIIGLLKEYMRDLVEQARQESQAQEAFGFSAPPYQPDHA
                     IADLLAILDDRIESEGVQVGLPIEFLHEMWRRCNEARGEITDRVWLETNIGLSGPSKA
                     RVRELTYASVIDCLEREPSGE"
     gene            3429..4226
                     /locus_tag="PKK81_06230"
     CDS             3429..4226
                     /locus_tag="PKK81_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV32504.1"
                     /translation="MSNHTAVEHFEISLQTPSGEISTAVGVPTSFVPITAILPLMRSL
                     GEEAQVLEQRRLLQAGERVSCEKGCAACCRMLVPISVPEAFALTNTIDRLDQNERNRL
                     LSKLDQAQQQLARAGILKQLSSLADSSEPLSDEAIEPLNRAYYALRMPCPFLDNETCS
                     IYADRPAACRELAVTSPATECQDMTTPTVQPVPVAVRLSTALSLLWADLTGTAPRLIP
                     LPLAVDWARRHKEEQTNQWTGTELFEKAMDKIWRYLSQEKARRSNAG"
     gene            4310..4930
                     /gene="thrH"
                     /locus_tag="PKK81_06235"
     CDS             4310..4930
                     /gene="thrH"
                     /locus_tag="PKK81_06235"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNV32505.1"
                     /translation="MQNPVIVCLDLEGVLVPEIWVNFAIKTGIEELKITTREMPDYDA
                     LMTRRLSILDQHGLTLADIQSVIDAMGPMEGAAEFIAWLRERTQVIILSDTFYEFALP
                     LMRQLGYPTIFCNQLEIGTTGRIVHYKLRQPNQKKHAVAALKNLNFRVMAAGDAYNDT
                     AMLGEAHAGFFFRPPDHLPKEFPQFPVTKTYAELQEQFRKAGNLRE"
     gene            complement(5009..6871)
                     /locus_tag="PKK81_06240"
     CDS             complement(5009..6871)
                     /locus_tag="PKK81_06240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNV32506.1"
                     /translation="MDDLSTQLSRTFGFSAFRAGQREVMEAVLARRDAMAVMPTGQGK
                     SLCYQLPATLLPGVTLVISPLIALMQDQVTSLTARGIAAAAFHSGLSEQERDRVVLDL
                     KLRRLQLLYLAPERMQHERFLRLLRSLWVSLLVVDEAHCISQWGHDFRPDYLNIGRLR
                     RELENPPCLALTATATARVQADLCDRLSLQDPLRLVTGFRRSNLALSVRLCRSRQEKL
                     ATLDRVVREYETGTILIYCATRRAVEEVAACLGQSQSSVGYYHAGLSDEERRKVHDEF
                     RVGTVRILAATNAFGMGIDKSDVRLVVHFDIPGSLEAYYQEVGRAGRDGRPAACLLLF
                     HERDVATQEYFIQQASKESGSSARADRMKTLLQDLLEYVSVPTCRQLAILDYFSDEAE
                     RALGPCGLCDRCVVTPQLSAHVVGDEASCAKAVLAAVSWCMGRFGVSRIVDMLRGSRS
                     KALLTYGAEECPGYGGYHAWSKTALTRLVKALIDAGYLQVEGLEYPTLDLTRKGQEVL
                     KGISPLVLSEQAEDPAASTLKPQGSGRGSSMAPLHTASVDPQLFERLRQLRRELAEEE
                     GVAPFVIFHDKTLRNIAGYKPVTLAALLEIPGIGEVKVERYGRRVLGVVNEACD"
     gene            complement(7087..7518)
                     /locus_tag="PKK81_06245"
     CDS             complement(7087..7518)
                     /locus_tag="PKK81_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32507.1"
                     /translation="MMKKLMFVTLGIAALAVGGQPVAAQAQVADAIQAVNDAIVELTD
                     APGLGKRTTVDLAKRWGSDRSVIDSATAKLQDGLSKAQSGGVGADAMRQLKLAVEYGK
                     ARLHKEARLSAQGALRHLCVANQDQGPGCDTVPKFGSYTAP"
     gene            complement(7599..9257)
                     /locus_tag="PKK81_06250"
     CDS             complement(7599..9257)
                     /locus_tag="PKK81_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNV32508.1"
                     /translation="MKRIHGRTRWGRLAAIFGAAAVTALQCLTVPAFAGFELPPGERI
                     TNLPAIPRNMPQKEAYELYDPVIGRNFDIKNLWMRADLRVRPEMRNNACFGGAQGAGG
                     TCNSFGTRGTAPGGGNKGNDMFVQQWMRLGIGYDLSPDVNFYVEIIDSATWGGNGSAV
                     NAGNAGDPLNHNGGAAGGSGNGGRLGVRAAYMLVRNLADIQGLSVKVGRQYVIFGNHS
                     LFGHFDWANTGYSHDGVMFAYQTKKWDSYFGWFRNSESDLGQAAPVGSGAANIAGTGA
                     QDAQRDADMFIFYNQIKMVPGMVIEPFYVLYQNRYGSADNAGQGLGTAKHSNQTRHMI
                     GNRIEVRKGGFDFSNEIAYQFGQMGQAGACAGEQKCLHINAWATRNWIGYTFYDTAWK
                     TRIAFNLDYASGDSRNNTCGATPGSCKTANTFENFFPTNHIHMGYMDVQAWKNMLSPS
                     VNLQARPSARDHIELWYTNLNLANSKDCWYRGAQGCYVFSNANNTKTHIGDEIDVAYT
                     RMFADGKVALQAAYGTIFSGGYLTSTLNQTQNQHWAYMSLWMNF"
     gene            complement(9508..9807)
                     /locus_tag="PKK81_06255"
     CDS             complement(9508..9807)
                     /locus_tag="PKK81_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNV32509.1"
                     /translation="MFGLGAGEILIILVIAFLLFGPKQLPEIGRQVGKAVKGFKETAD
                     DLKKTVEPELNMIQQEMKMVEQDFESSMKEAEEQINHATSGVEHGAEESGLPKQA"
     gene            complement(9902..10186)
                     /gene="tatA"
                     /locus_tag="PKK81_06260"
     CDS             complement(9902..10186)
                     /gene="tatA"
                     /locus_tag="PKK81_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNV32510.1"
                     /translation="MFGSFGWMELLLILIIVLIIFGAGKIPQLGEGLGKAIKGFKKSV
                     HEADAIDVTATEAEPAPAQPAAQIQQTGQPATPPPAQQAGAAPPPRTTQG"
     gene            complement(10458..11942)
                     /locus_tag="PKK81_06265"
     CDS             complement(10458..11942)
                     /locus_tag="PKK81_06265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV32511.1"
                     /translation="MEQHLMTQLEDPDALPQPLGEYKPVDYWQAHINTLFYQLRGDQQ
                     RSFYQTFTSADYRLAHALAADYFEQVTKRDKKVAANRVTSNGPTATPSTDATPQAQLT
                     VMEWGPGNGNLAACFLSHLQRLDKGGRVYPRVRYLLVDSQAHALERARAHPDLAPHMA
                     KVESLCAEVEDLATIADGTVDRILCNELWNELATKLMVKKGGEFEEEHLRPNLNERKA
                     AAIADWSGFVRAFEAKDIERLKQFPPFLDDLIWEREYHKVDWKDVPYRKTITEFMKAI
                     DDEVLVPVNLGAFASLKEAKRVLAQDAVGFSSFDAGTADMEVLNDPDKPCYGQFGGQY
                     SFMVNLALIQAVAKHLGLNAVTIETQREFVGSRLGTNVMTLMDLLACHPMVGSKVQPW
                     ELDRLTVKTIRTLNETYESPYQRKIEFPLRSEMPAEERDAAQGILLSLKPNGIPDTIA
                     YVTEEELSQAQPELENLGYEREAVLMALGAPPSPVEYYHFACRP"
     gene            12130..12705
                     /locus_tag="PKK81_06270"
     CDS             12130..12705
                     /locus_tag="PKK81_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32512.1"
                     /translation="MRQLLWFCCPIFLLSGSLLWAEDLPLTRNVPIPDFQNRTESAKP
                     YNFDAPPEGMFRHIVLAEGFDEELGFQRTHEIVPVRPTEQFAAGSRPVFIVFELHQHY
                     QSFQVFGRCYPEEVTGLDGRTVVAEDAMYIALEDQTGYLKLFPTESGWKPGRYKVEIH
                     AGEQINDMSLMGTMRFTITASSDSGAPARSR"
     gene            12827..13558
                     /locus_tag="PKK81_06275"
     CDS             12827..13558
                     /locus_tag="PKK81_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNV32513.1"
                     /translation="MQWDILATILLTATIQSLFGVGVLLFGTPILLVLGYDFITALTI
                     LLPISLTINLLQVSKDYRHVQVPFYRQVLTLSIPCVVLCLLLVTTLKLNIGSIVGLFL
                     IVAALKSIYVPFNRLIESLVRYERTYFIVMGVVHGLTNLGGSLLTAIIHSKQYDKDQT
                     RATTALSYGTFALFQLVTLGISLGPSAIATSDTALYMAVGVSMFLATELLLYSKMSTE
                     RYRTTFAAFLFLSGTALIFKSLFLR"
     assembly_gap    13768..13867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      13872..13947
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            13968..>15322
                     /locus_tag="PKK81_06280"
     CDS             13968..>15322
                     /locus_tag="PKK81_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV32514.1"
                     /translation="MNQRPTTTPTYPQYQLTEILRDIFAHLGMLLKLERSILGIIASY
                     AVAIGFFLLCVPIAVQELVSTFSFAMEPRMIFTLALFVASSLTGVAAFRVLQARAVET
                     FQQRIYTRIAIGFTRLLPRLRDDTFATPQAYRFMEADLLTRALVAMVADLFNVAVVGT
                     IGVTMLILFHPFFVLYILVLILGFVGLLTLFGRGGFFITLEMSRLHYDIFGWIQNIAH
                     NLPHLRAAGDSPYLLERTDTLTRMYARIRQRRSDTLTGRQYKAAALWQVAGHSGLLIT
                     AGLLVADGQLTVGQFAAAEVLVGNLLINMDTLARRMVAMFFTFVSCREMAAVFSLPTE
                     EDGAKEDVPAAQFGAAGIHLTCRNLTYTGPDGTPIFENVSLDVAPGEKVAILCNTNNA
                     KTALAKVLAGLHPSASGFVRYNDMNLIEVKRDSISRVRGLVLDSHPTLLDGTLEDNIT
                     LG"
BASE COUNT         3224 a         4815 c         4038 g         3145 t
ORIGIN      
        1 gcaccagcac ctgaggcatc cgtcacctca cagcccatta tactccttcg gacgaaaaga
       61 accgcctttt ctgaacgaaa ggtggggaca gagctgggcc ggggagacga atgaggggaa
      121 tattcttctg aactgctgtg gattacgacg acgccaggcg cgaacgggac gggttaatca
      181 gagctgatga ttttgaggaa ggattgtcca tcgcctcaaa caagaggcaa cacttgagac
      241 gaccacagac accgagtaat ttggggtcat cgacaggaag ccccaacacc cgtgcttgct
      301 tgacagtcac cggtttcact tcagtgagga acgcggcaca acagagtacg agaccgcatg
      361 tatcgatccc gcccaaccga ctggcttcgt cgcgtacccc aacttgacgc atttcaatac
      421 gcccgccgaa tcggcgcgcc aacaagcgaa ccaactcgcg aaaatcgatt cgatcctcag
      481 cggtatagac aaaggtcatc tgccggcgat gaaacgagca gaacacctcc acaagtttca
      541 ttctgagtcc cagggccgcc gccttctccc gacacgcttt cattccctcc gatgccaacc
      601 gttcgtaccg atcgatcgtg gccacatctt cggttgtcgc cttcctggtg atcggctgaa
      661 gcacgcgcat gggcggaatg aacggcatca cctgaggagc cccgtaaacg accccgtagc
      721 tcaactcgcc ggccacttcg agcagtaccc gatcccccat caccagggac gcctctccgg
      781 catcgaattt cttcacctcc ccacggtcac ggatctttac gcccacgatc cgaacgactg
      841 cgggataggg gttcgcgagt tcttgcgcaa gaagtttctc gaccatgggg gcatgataca
      901 ccatccaatc tttcaattga aatgctcagc gctgtctcat cgccatcgaa aattgctcaa
      961 ccaccaccga gcagccatac agcgaccaag cagcaatctc tcgcaaacca ctgatagtaa
     1021 tgattattta gacaggaaga cgaaagagaa aatatgttag gctattccga agcaggaatt
     1081 cttggctgag gccacctatt tgatacgacc gtgaggggca tggtgcgaat accaaacaaa
     1141 gtcatccttc cattcggcta ccacatcatg atcaggcagg tcacggactc cgagatggac
     1201 cggcaggatt cgaatgcgga tgggatttgg gacaatgaag ccaagaccat ctatattcgc
     1261 aaacgactac ccgtcacgcg gcggagatac atcctcgccc atgaactggg acatgcctgg
     1321 ctcgactggc aacatcgata tctggatgat ggaaaggctc gtagttgacc cgtgctgaac
     1381 cgaggacacc tcccgtccgg aagtgagttc ttcccggacc gtgctagctg tccgcctgta
     1441 cctcctccag cctgataatt tcattccact cggccgcccg caccggctga accgacagcc
     1501 gcgatccctt tcgaagcaat tccattccct tcagaccagc cttcgcacgc aacaggtcca
     1561 acgacagcgg cgcgaggaat ttgcgaacaa accggatatc gaccatgtcc cagcgaggct
     1621 gagccggatt gccggccgga tcgtagtgcg aatcccgctt atcgaactgc gtggcatcgg
     1681 gataggccgc tctcaccact tccgcaactc caaccaccgc gggcggattg gcattactgt
     1741 ggtaaaagag gacctgatcc ccgattttca ttgcccgcat aaagttacgc gcctggtagt
     1801 tccgtacacc atcccatgaa gtcgtgctct tcggtgaccc agcaagatca tcgattgaaa
     1861 agaccttggg ttcggatttc atcaaccaat agtgccgtcc gtctgccatc gaatcctcct
     1921 caacacttgg tgtctgtacg ccctcagtgt atgctgagca ctctgtttcg cctattaacc
     1981 gggagcgtcc atggacctca cgcccatgtg gttcggtgtg tataaggcct tgaaatacct
     2041 gctctacccc ttgtcatgga ttctcatcgc cggactcctc gccctgttga ccgcctgcct
     2101 ccccgtgtcc cccgggcgga ggacttgggt caggcgcctc ttgttcctca ccgtgctact
     2161 gttgctgctg acggccactc ccgtactatc cctcctatac attgcgctgc tcgaaacgca
     2221 gtatccatca ttccagccca cttccacatc gaagttcgac gcggtggtgg ttctggcagg
     2281 aggcgtcttt cagaagggat cactccgccc agccgatgag gtttctgatg cctcccgcca
     2341 gcgtacgggt tgtggcgcag acttgtggca acagaatctt gccccaaaac tgttgctcac
     2401 cgggggcgac gccactgtat tccgaaccgg tctgctggaa tcccacgaga tgaaacggtg
     2461 ggcccttcgc cttggtgtgc cggagtccgc gatcctgatc gaggaacaat ccaggaccac
     2521 ttatgaaaac gccgtgcaga ccaaagccat acttgggtca ggacgcatca tattggtgac
     2581 cgcagcctat cacctgcccc gcgcagtcag actcttcgaa aagcaaggtt ttgtcgtcac
     2641 cccgtttccc tgcggctacg aatctcaaca cactccacaa caagcctggg aacaggccac
     2701 cctctttgac ttcctgccca cagccaaggc cctgctcatc accacccaag ccgtcgacga
     2761 agtggcaggg atcatcgttt attggcttgc cgggaaattg taggatcccc ccaagactct
     2821 agcctgctga gactccacac agccaaaaga ataagggccg tgtagctgtg cgacggaatg
     2881 ctgtcgttcg tctggctcac tcgccggaag gctcacgctc aagacagtcg atcacagagg
     2941 cgtaggtcag ctcacgaaca cgagccttgg aaggaccaga caggccgata ttggtttcga
     3001 gccacacacg atcggtgatc tcaccgcgcg cttcgttgca gcgccgccac atctcgtgca
     3061 aaaactcaat gggcagccca acctgcaccc cttccgattc gatgcggtca tctaaaatag
     3121 ccagtaaatc ggcgatggca tggtccggct ggtacggagg tgccgagaag ccgaacgcct
     3181 cttgagcctg cgactcctgc cgcgcttgct cgacaagatc ccgcatgtat tctttcagca
     3241 atccaataat gtggcctgac aaacgcatcg acgacagagt agccgcaaca gtcctcccgg
     3301 ggcaagactg agggctatga ggctttggtt gaccactgat tctgaggttc tctatgatag
     3361 cgcctgcgca ggatggcacg aatgccatcg cgcaagtgac tcccattcgg acttaaagat
     3421 gggtacccgt gagtaaccac actgccgttg aacactttga aatctctctg cagactccaa
     3481 gcggagaaat cagcactgcc gtgggcgtgc caacgagctt cgtaccgatc actgcgattc
     3541 tcccgctcat gcgcagtctc ggagaagaag cccaggtcct ggaacaacgc cgattactcc
     3601 aagccggcga gagggtctcg tgcgaaaagg gctgcgccgc ctgctgccgg atgctcgtac
     3661 cgatttcggt gccggaagcc tttgctctca ccaacaccat cgaccggctt gaccagaacg
     3721 agcggaatcg cctgctgtcc aaactcgacc aggcccagca acaactggct cgcgccggta
     3781 tcctgaaaca actctcttcg ctggccgatt cttctgagcc gttgagtgac gaggcgatcg
     3841 agccgttgaa ccgggcctac tacgcgcttc gcatgccctg cccctttctc gacaatgaaa
     3901 cctgcagcat ttacgccgat cggcctgcag cctgccggga gcttgctgtc acgtcgccgg
     3961 ccacagaatg tcaggacatg accacgccga ccgtccagcc ggttcccgtc gccgttcgcc
     4021 tcagtacggc tctgagcctg ctctgggcag acctgaccgg aacagcaccc aggctgatcc
     4081 cgctcccgct ggccgtcgat tgggccagac ggcataagga ggagcagacc aaccaatgga
     4141 ccggcacaga actattcgag aaggccatgg acaagatctg gcggtatctc agtcaagaaa
     4201 aggcccgtcg tagtaacgcg ggatagggca gaccgctggt ttgtccctat cactacccgt
     4261 taccgaaaag cccacttttc accacccatc aattcccgca ggaggatgaa tgcagaaccc
     4321 cgtgattgtg tgtctggacc ttgagggcgt gctggtgcct gaaatttggg tgaactttgc
     4381 catcaagacc ggcatcgaag agctcaagat caccacccgc gagatgccgg actacgatgc
     4441 cctgatgaca cgccgcctga gcatcctaga tcagcatggc ctgaccctgg cggacattca
     4501 atccgtgatc gacgccatgg ggccgatgga aggcgcagcc gaattcattg cctggctgcg
     4561 ggagcggaca caggtcatta ttctgtcgga tacgttttat gaattcgcgc ttcccttgat
     4621 gcggcagctc ggatatccaa cgattttctg caatcaactg gagattggga cgaccggcag
     4681 gatcgtccac tacaaactcc gccagccgaa ccagaaaaaa catgcggtgg ccgcgttgaa
     4741 gaacctcaat tttcgtgtca tggcggcagg agacgcctac aacgacaccg ccatgctggg
     4801 agaggctcac gccggattct ttttccgccc ccctgaccat ctcccgaaag agttccctca
     4861 gtttccggtg acgaaaacgt atgccgaact gcaggagcaa tttcgaaagg cagggaatct
     4921 gagagaataa tagccaaatg gctacgaaat cattcttccc cggcaaaatg gcattgaagg
     4981 ggtcattaga caataacgag aagacggctt agtcgcaggc ctcgttcacc acgcccagca
     5041 ctcggcggcc ataccgctcc accttgacct cgccgatccc ggggatctcc agcagggcgg
     5101 caagcgtcac gggtttgtaa ccggcgatat tccgaagggt cttgtcatga aaaataacaa
     5161 acggcgcgac cccttcttcc tcggccagct cacgccggag ttgacgaagc cgttcaaaga
     5221 gctgcgggtc gactgaggcg gtatgcagag gcgccatcga gctacctcgc ccgctccctt
     5281 gtggtttcag cgtcgacgct gccggatcct cagcctgctc gctcaatacc agaggactga
     5341 ttcctttcaa cacctcctgt cccttccgcg tcaggtcgag cgtgggatac tccagtcctt
     5401 ccacctgcag atatcccgca tcgatgaggg ctttcaccag acgagtcaac gccgtcttcg
     5461 accatgcgtg atacccccca taccccggac actcttcggc cccataggtg agcagcgcct
     5521 tcgatcgact cccgcggagc atgtcgacaa ttcggctcac accgaatcgg cccatacacc
     5581 aggagaccgc cgccaggaca gccttcgcgc aggaggcctc atcgccgaca acgtgcgcac
     5641 tgagctgtgg cgtcaccacg caacgatcac aaagcccgca gggcccaagg gcccgttcgg
     5701 cctcatcgct gaagtagtcg agaatggcga gttgccgaca ggtcggcacc gacacatact
     5761 ccagtaaatc ctgtagcaac gtcttcatgc gatcggcgcg tgctgaacta ccggattctt
     5821 tcgacgcttg ctgaatgaag tactcctgag tggccacatc ccgttcatga aacagcagta
     5881 ggcaggcggc aggtcggcca tcacgccccg cccggcccac ttcctgatag taagcctcca
     5941 ggcttcccgg aatatcgaaa tgcaccacca accggacatc cgatttatcg atccccatgc
     6001 caaacgcatt ggtcgccgcc aaaatcctca ccgttcccac acgaaactcg tcatgcacct
     6061 tccgccgctc ttcatccgac aagccggcgt gatagtaccc gacggacgac tgcgactgcc
     6121 ccaggcaggc cgccacctcc tccaccgctc gacgcgtggc gcaatagatc agaatggtgc
     6181 ccgtttcgta ttcacgcacc acacgatcca atgtcgcgag cttttcctgg cgggaccggc
     6241 agaggcggac agacagcgcg aggttagagc gtcgaaagcc ggtcaccaag cgaagcgggt
     6301 cctggagtga caatcggtcg cacaaatctg cctgcacgcg cgcggtcgcc gtggccgtca
     6361 aggccaggca agggggattc tcgagttccc ggcgcaaccg gccgatgttc agatagtcag
     6421 ggcggaaatc gtgcccccac tgggaaatac aatgcgcttc gtcgacgacg agtaacgaca
     6481 cccacagcga acggagcaga cggagaaacc gctcatgctg catccgctcc ggagcgagat
     6541 agagcaactg caaccggcgc agcttgagat ccaataccac tcgatcccgc tcctgctcgg
     6601 aaagcccgga gtggaacgcc gccgcggcaa tcccccgcgc cgtcaaacta gtgacttgat
     6661 cctgcatcaa cgcaatgagc ggagaaatca ccagggtcac cccgggcaac agggtggcgg
     6721 gaagctggta acagagcgac ttgccctggc cggtcggcat gaccgccatg gcatcgcgac
     6781 gggccagcac ggcttccatg acttcgcgct gccccgcgcg aaaggccgaa aacccgaatg
     6841 tccggctcag ctgtgtgctc agatcgtcca cgatgaggaa agaaacgcac gaacggtgat
     6901 gaagattacg agtgcgccga gtataaggcg atcgactgac tgcgggcaag ccagcgctct
     6961 caccgtaacc cgttgcccca cccatgcagg aaaaaagaag ccccgccggg gatcacccgg
     7021 cggggcttca ctcttcgaga caggcagggg atctccctca cgcctcgcac tatgcaggtc
     7081 gcactcctag ggagcggtat agctgccgaa cttcggcacc gtatcgcaac caggcccttg
     7141 atcctggttc gccacgcaca agtggcgcag agcgccctgt gcggacaagc gggcttcttt
     7201 gtgcaagcgg gccttgccat actccaccgc cagtttcaac tgacgcatgg catcggcacc
     7261 aactccgcca gactgagcct tgctgagccc atcctgcagc tttgccgtgg cgctgtcgat
     7321 gacggaacgg tcggaccccc atcgcttcgc cagatcgacc gtcgtgcgct tgcccaagcc
     7381 gggcgcatca gtcaactcga cgatggcatc gttcactgcc tgaatcgcat ccgcgacctg
     7441 agcctgcgca gcaaccggct ggccgccgac cgccaacgct gcaatgccca gcgttacgaa
     7501 catgagtttt ttcatcatga tctcctttgt cttgctttcg ctctcagcct acggcactca
     7561 gcgctgatgg ctgacagcaa tgagctgaag tcttctcctt agaagttcat ccacaacgac
     7621 atataggccc agtgctgatt ttgcgtctgg ttcagggtgc tggtcagata gccaccgctg
     7681 aaaatcgtgc cgtaagcagc ctggagggcc accttgccgt ctgcgaacat tctggtgtac
     7741 gcgacatcga tttcatcacc gatatgcgtc ttcgtgttat tggcattcga gaagacatag
     7801 cagccctgag cccctcgata ccaacaatcc ttggagttgg cgaggttcag gttcgtgtac
     7861 cacaattcga tatggtcacg agcggaagga cgagcctgca agttgacgga cggtgagagc
     7921 atgttcttcc acgcttgcac gtccatgtag cccatgtgga tgtggttcgt cgggaagaaa
     7981 ttttcaaagg tattggctgt cttacaagaa cccggcgtcg cgccgcaggt attgtttctg
     8041 ctgtctccgg aagcatagtc gaggttgaac gcaatacgcg tcttccacgc cgtgtcatag
     8101 aacgtgtagc cgatccagtt tctggtcgcc caggcgttga tgtgcaaaca tttctgttcg
     8161 cccgcgcagg ccccagcctg gcccatttgt ccgaattgat aggcgatttc gttgctgaaa
     8221 tcgaacccgc ccttgcgcac ttcgatccgg tttccgatca tatgccgggt ctgattcgag
     8281 tgcttggcgg tcccaagtcc ctgccccgcg ttgtccgccg aaccataacg gttctggtac
     8341 aatacataga acggctcgat aaccattccg gggaccatct tgatctggtt gtagaaaatg
     8401 aacatgtcgg catcgcgctg ggcatcctga gcgccggtgc cggcgatatt cgctgcacca
     8461 cttccgactg gagctgcctg accgagatcg ctttccgaat tacgaaacca gccgaagtag
     8521 ctgtcccact tcttggtctg ataggcgaac atcacaccgt catgtgaata accggtgttg
     8581 gcccaatcga agtggccgaa cagagagtgg ttgccgaaaa tcacatactg gcgaccgacc
     8641 ttcacgctca agccctggat atcggccaga tttctgacca acatataggc tgctcggaca
     8701 cccaaacggc ctccgttgcc cgaaccaccc gcagccccac cattgtgatt cagcggatcg
     8761 ccggcgttcc ccgcattcac ggcgctgccg ttaccgcccc aggtggcgct gtcgatgatt
     8821 tccacataaa aattcacgtc cggagacagg tcatagccga tgcccaaacg catccactgt
     8881 tgcacgaaca tgtcgttgcc cttgttgccg ccgccgggag cggttccgcg ggttccaaac
     8941 gagttacatg ttccgccggc cccttgggcg ccgccgaagc aggcattgtt ccgcatttcc
     9001 gggcgcacac gcagatcggc gcgcatccag agattcttga tgtcgaagtt ccgaccgatg
     9061 accgggtcat acagttcgta cgcttctttt tgcggcatgt tgcgaggaat ggcaggaaga
     9121 ttcgtgatac gttcgcccgg gggaagttcg aatccggcaa aggccgggac tgtcagacac
     9181 tgcagagccg tcactgccgc agcgccgaag atggcggcca accggcccca cctggtccgt
     9241 ccatggatgc gtttcatagc gttcctcctg tgaattggat ggcaacccat tgtgtaccca
     9301 ctcacgccgg gtgccgccta tacatccaac cgctattgca ctacaaagcc cactttgtgt
     9361 gtgatgtcgg ggggctcgaa cctgaatggt gcccaccatt cggccccgat cctcgcttca
     9421 tcctgaaacg agctcctcac accgtaaggt cttgtattgt tgctgtggtc acctcccttc
     9481 acagccgtag agcggatatg aatggtatta ggcctgcttg ggcaaacccg attcctctgc
     9541 cccgtgctct acgcccgatg ttgcgtggtt aatctgttct tccgcttcct tcatcgacga
     9601 ctcgaaatcc tgctccacca ttttcatttc ctgctgaatc atgttgagct ccggctcaac
     9661 cgtcttcttc agatcgtccg ccgtttcttt aaatcctttc acggccttgc ccacctgacg
     9721 cccgatctca ggcaactgct tgggcccgaa gagcaggaac gcaatgacca ggattatgag
     9781 aatttcacca gcgccaagac cgaacatggt gttccatact gaggggggtg tgctgaagac
     9841 cgagcggcgg gactccgaga tcacatcgcc gtcattcggt ctgcatcctc agtttcgcaa
     9901 gttacccctg cgtcgtccgt ggcggtggag ccgcaccggc ctgttgcgca ggcggcggtg
     9961 tagccggctg tccggtttgc tggatctgtg ccgcaggctg agccggggca ggttccgcct
    10021 cggtggccgt cacgtcaata gcatctgctt cgtgaaccga tttcttgaac cccttgattg
    10081 ctttacctaa tccctcaccg agctggggaa ttttccccgc accgaagatg atcaggacga
    10141 tgatgagaat cagcaacagc tccatccagc caaacgaacc aaacatggta ctgtatgctc
    10201 cttgttacag ctgtgtgatt attcgcacga cgtatattca cgcactcacg actgcaaagc
    10261 cctctcgtga agaacgtcgt ctgagatgat cttgcctgcg agaccccatc ccgattgaat
    10321 gctcgcgatg cacggagggg agacgggtct tctcttcgaa atcgcgggaa aggctataga
    10381 aatggaatta attagtcaag ctctttctcc tacagacatc acctggtggc ccgtcctgcc
    10441 gcacgatcca gcgcgggtta cggccgacat gcaaagtgat agtactcgac aggactcgga
    10501 ggcgccccga gcgccatcaa caccgcctcg cgttcatagc ccagattctc caactccggt
    10561 tgtgcctggc tcaattcttc ttcggtgaca tacgcgatgg tatcgggaat cccgttcggt
    10621 ttgagcgaga gcagaatacc ctgcgccgcg tcgcgctcct cggccggcat ctcactgcgc
    10681 aacgggaact cgatcttgcg ttgataggga ctctcataag tctcgttcag cgtgcggatg
    10741 gttttgactg tgaggcgatc cagctcccag ggctgaacct ttgagccgac catcgggtgg
    10801 caggccagca agtccatcag cgtcatcaca ttcgtcccca gccggctccc gacgaactcc
    10861 cgctgcgttt cgatcgtcac cgcgttcagc cctagatgct tggccacagc ctgaatcagg
    10921 gccagattta ccatgaagct gtactggcca ccgaactggc catagcaggg cttgtccgga
    10981 tcattgagga cttccatgtc agccgtcccg gcatcgaagc tgctgaatcc cacggcatct
    11041 tgggccaaca cccgcttcgc ttccttcagg gatgcaaacg cgccgagatt gaccggcacc
    11101 aacacctcat cgtcgatcgc tttcatgaac tcggtgatcg tcttgcgata gggaacgtct
    11161 ttccagtcca ccttgtggta ctcgcgctcc cagatcagat cgtccagaaa gggcggaaac
    11221 tgcttcaagc gctcaatgtc cttcgcctca aaggcacgga caaatcccga ccaatcggca
    11281 atggccgcgg cctttcgctc gttgaggttc ggccggaggt gctcctcttc gaactcgccc
    11341 cccttcttca ccatgagctt ggtcgccaac tcgttccaga gttcgttgca cagaatgcga
    11401 tcaacggtgc cgtcggcgat agtcgccaag tcctcgacct cggcgcacag cgactccacc
    11461 ttcgccatat ggggagccag atccggatga gcccgcgccc gctccaacgc atgggcctgg
    11521 gaatcgacca acagataccg gactcgtgga tacacccgcc cgcccttgtc gagacgctgc
    11581 aggtgactga ggaagcatgc ggccagattc ccgttacccg gcccccactc catcaccgtc
    11641 aactgtgctt gaggagtggc gtcggtggac ggcgtcgcag tggggccgtt tgacgtcaca
    11701 cgattggcgg cgactttctt gtcgcgtttg gtgacctgct cgaagtagtc ggcggccagc
    11761 gcatgtgcca atcgataatc ggccgaggtg aatgtctgat agaagctgcg ttgctgatcg
    11821 ccccgcagtt gataaaacag ggtgttgata tgggcctgcc aataatcgac cggcttatac
    11881 tccccgagcg gctgcggcag cgcgtcggga tcctctaact gcgtcatgag gtgctgttcc
    11941 atgtctgtcc ccccgtagaa aggaggaaag tctaacagga agcaattttc acccgcaacc
    12001 tgccccgcac gattcatcgc tcccgcctcg cctcttagac ctccctctta ccaagcggac
    12061 cgtccggcca gaggcccggc gcagagcctc tccttgacag gccggccggg cggagtgtac
    12121 caagacggga tgaggcagtt gctctggttc tgctgtccca tcttcctgct ctccggctcc
    12181 cttctctggg ccgaggatct cccgcttacc cgcaatgtgc cgattcccga tttccagaac
    12241 cgcaccgaga gcgcgaaacc ctataacttc gacgccccac ccgaaggaat gttccgccac
    12301 atcgtgctcg ccgaagggtt tgatgaggag ctcggatttc aacgaaccca tgaaattgtt
    12361 cccgtcagac cgaccgaaca gttcgccgcc ggctccagac cggtcttcat cgtcttcgaa
    12421 ctccatcagc attaccaatc gttccaagta ttcgggcgct gctacccgga ggaagtgaca
    12481 gggctggacg gaagaaccgt ggtcgctgaa gatgcgatgt atatcgcctt agaggaccaa
    12541 accggctatc tgaagctgtt cccgacggaa agcggctgga aaccaggacg gtataaggtg
    12601 gaaatccatg ccggcgaaca gatcaatgac atgagcctga tgggcaccat gcgcttcacc
    12661 atcaccgcct cctcagactc gggggcccct gccaggagcc ggtaaaccac gccccgcctg
    12721 tgggctgatt gacgcccgcg ccaaagctgg gatataccgg gctaccggac cgggagttga
    12781 gagcctgtat cttggacacg ttcggtccga gaggagcatc atcgccatgc agtgggacat
    12841 tcttgcgacc atcctcctga cggccaccat ccagtcgctc ttcggcgtgg gagtgctgct
    12901 gttcgggact ccgatcctcc tcgtgctcgg ctatgacttc atcaccgccc tgactatcct
    12961 cctcccgatc tcgctgacca tcaacctgct acaagtcagc aaggactacc gacacgtcca
    13021 ggtgcccttc taccggcagg tcctcactct gtcgattccc tgcgtggtgc tgtgtctcct
    13081 gctggtcaca acactcaagc tgaacatcgg cagcattgtc gggctcttct tgatcgtcgc
    13141 ggcgctcaag agcatctatg tgccgttcaa tcggttgatc gagtcgctgg tgcgatatga
    13201 gcgcacctat ttcatcgtga tgggcgttgt tcatggcctg accaatctgg gtggctcgct
    13261 gttgacggcc atcatccaca gcaaacagta cgacaaagat cagacccgcg ccacgaccgc
    13321 cctgtcctac ggcacgttcg cgctgtttca actcgtcacg ttgggcatct cccttggtcc
    13381 ttctgcaatt gccacctccg acaccgcact ttacatggca gtgggcgtga gcatgtttct
    13441 ggctaccgaa ctgctgctct atagcaagat gagcaccgaa cgataccgca ccaccttcgc
    13501 cgcatttctc tttttgtccg gcacggccct gatcttcaag tccctcttcc tccgctaacc
    13561 ggatgcggaa aaactccgcc agcgtcgtac tccaccgaca atggaccgat cagctctcgg
    13621 cttagctcac gccaaaccaa cgaatatgag gccatgaagc gggctatgtg ttgacgaact
    13681 agagagtctc ggtctagact tcgcactaat acagccagtc cacccggtga tggagttcac
    13741 cgagaaccgc cggtcgcgat cctcgcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnagc cagtccaccc ggtgatggag ttcaccgaga accgccggtc gcgatcctcg
    13921 cattcggctg ataactccta ccagcgccca acgcggtagg agtttctgtg aaccagcgcc
    13981 ccacaacaac cccgacctat ccccagtatc agctgaccga gatccttcgc gatattttcg
    14041 cccacctggg catgctgctg aagctggagc gatcgatcct gggcatcatc gcctcgtatg
    14101 cagttgccat cggcttcttc ctcctctgtg tccccattgc cgtccaggag ctcgtcagca
    14161 cgttctcctt tgcgatggaa ccccgcatga tctttacact ggccctgttc gtcgccagtt
    14221 cgctgaccgg cgtcgcggca ttccgtgtgc tgcaggcgcg cgcagtggag acattccaac
    14281 agcggatcta cacgcgcatc gcgatcgggt tcacacgtct cctgccccgc ctccgcgacg
    14341 atacgttcgc cacgccgcag gcctaccggt tcatggaggc cgacctgctg acacgcgcat
    14401 tggtagccat ggtggcggat ctgtttaacg tcgccgtggt cggtaccatc ggcgtgacca
    14461 tgctgatcct gtttcacccc ttcttcgttc tctacattct cgtactgatt ctaggctttg
    14521 tgggattact gaccctcttc gggcgcggcg gcttcttcat cactctggag atgtcgcggc
    14581 tccactacga tatcttcggg tggattcaaa acatcgcgca caatctcccc catctgcgag
    14641 ccgccggcga cagcccctat ctccttgaac gaaccgatac actgacccgc atgtatgcca
    14701 gaattcgaca gcgccggtcc gacacactca ccgggaggca atacaaagcc gcagcgctct
    14761 ggcaagtcgc cggccacagc gggctgctca tcaccgccgg actcctggtg gcggacggac
    14821 aacttaccgt ggggcaattc gccgccgccg aggtgttggt cggcaacctg ctgatcaaca
    14881 tggatacctt ggctcgacgt atggtcgcga tgtttttcac cttcgtgtcc tgccgggaga
    14941 tggccgcggt gttctccctg cccacagaag aggacggcgc gaaggaagac gtgccggcgg
    15001 cacagttcgg agcggccggc atccacctga cgtgccgaaa cctgacctat accggtccgg
    15061 atggcactcc gatctttgag aacgtgtccc tcgatgtcgc ccccggcgag aaggtggcga
    15121 tcctctgcaa cacgaacaac gcgaagaccg ctctcgccaa ggtcctggcc ggactccacc
    15181 cgtcggcatc cgggttcgtc cggtacaacg atatgaatct gatagaggtg aagcgcgatt
    15241 cgatcagccg ggttcgaggg ctcgtcctcg actcccatcc cacgctgctg gacggcacgc
    15301 tcgaagacaa catcacactt gg
//
LOCUS       DAOQRM010000054        14935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3066_length_14935_cov_14.467944, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000054 DAOQRM010000000
VERSION     DAOQRM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3066_length_14935_cov_14.467944"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1474)
                     /gene="lpdA"
                     /locus_tag="PKK81_06285"
     CDS             complement(53..1474)
                     /gene="lpdA"
                     /locus_tag="PKK81_06285"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNV32515.1"
                     /translation="MNHLAILGAGPGGYVAAIRAAQLGARVTVIENQALGGVCLNWGC
                     IPSKALLSVVELGDKAKKAKDFGIQLSGPVTYDPAVMVARKNKVVSTLVKGIATLFKT
                     WNIEHVEGTGELLDARTIRVTKPDGTETRVVADGVIIATGSSWPNLPLFPIDGTQIIT
                     SKQALDLSRIPVSLLIVGGGVEGCEFASLYSGLGTQVTLVELVPRLLPLEDEEISQMM
                     ERELKKRGVDIRTGVTVDQIVRQPDLVTAHLRDGLSLNVEQVLVSVGRGFNSRGIGLE
                     KAGVQVGTRGEIVVNDRMETNVPGVYAIGDVVGKAMLAHVASAQGKVAVENFMGRPRT
                     IDYDVIPTGIFTLPEIGRVGLTEQQARDRCVAAGKDPQQSVKVGRFRYGGLGKAQATG
                     DIQGLLKVVADAESDRILGVHILGAHAADLVHEAALAMHLGATVSRVAEMIHAHPTLA
                     EGLMEAMEDVHGHAIHLARKPSS"
     gene            complement(1482..1868)
                     /gene="gcvH"
                     /locus_tag="PKK81_06290"
     CDS             complement(1482..1868)
                     /gene="gcvH"
                     /locus_tag="PKK81_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNV32516.1"
                     /translation="MIPSNLRYHQEHEWVRAEGAQATVGISHFAQDALGDIVFIDLPK
                     VGAKVTAGQQIGEVESTKTTSTIYTPVSGTVAKINGDLKDHPEVVNSDPYGKGWMAVI
                     DLTAPAEVDQLMTAAQYETFLSKQKH"
     gene            complement(1964..3391)
                     /locus_tag="PKK81_06295"
     CDS             complement(1964..3391)
                     /locus_tag="PKK81_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32517.1"
                     /translation="MPAVVPPTETPAVAAETSLWYQASTAFSDGRYSAAIHLYERYLT
                     NYPKSRRALEAHWDLGQAYEQMGEVTAAIKEYRTLAGPEGAPLSAQNSYAERAQHRID
                     ALRNQPASPAGARSGHTALYVSFSNLPPMSQVEAWIRQLSAQGISAILMDASLESSFT
                     RPLIPTSPPTSSPLPTLPGGALFATSRGPVLTDWYGVMVPQAHELGISVYAVLDLYHA
                     PLSDHRPESKMLLYDPTRRKVHPWTQFDLLAPAVQQQMAQLLADLLKTGVDGLVFRSR
                     AENSFAYEVSDGVLRQFETQVHQPSSEVAQALRDRMALRPNEPAPNSDKTLWRWVGWK
                     ARQELDTLARFKKYLQKAVPRFRMVIEIHPEALSNPTSALVNYGEDAAEARRRGFDLL
                     LGGASREASDVRSFGGAFKGWSRDVIQSEKGEPQTLPQGWVLLRTPAEHGGGMFTGLA
                     QQADELRTPDIRHLVFVPDAPVVIP"
     gene            complement(3531..3950)
                     /gene="ndk"
                     /locus_tag="PKK81_06300"
     CDS             complement(3531..3950)
                     /gene="ndk"
                     /locus_tag="PKK81_06300"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNV32518.1"
                     /translation="MSERTLAIVKPDAVKKNAIGDIINRYEKAGLRPVAMRLMHMSKA
                     TAQGFYAVHKARPFFDSLCTFMSSGPCVVLVLQGDNAIKKNRELMGATDPAKAEQGTI
                     RAAHGANIEFNAVHGSDAPETAKFEIGYFFAEMELVG"
     gene            complement(4138..5169)
                     /gene="mtnA"
                     /locus_tag="PKK81_06305"
     CDS             complement(4138..5169)
                     /gene="mtnA"
                     /locus_tag="PKK81_06305"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNV32519.1"
                     /translation="MVPTVEWKDGAVRLLDQSRLPMHVEFLDCRDYRAVAQAIRDLKV
                     RGAPAIGVTAAMGVALGAQAVTATEYRDFATQVGEICDHLAASRPTAVNLFWAIGRMK
                     QKLAALNNHPVAGIKAELITESQTILDEDIALCKAMGQHGAALIQSGQTILTHCNAGA
                     LATAGYGTALGVIRAAWEQGKQIQVIADETRPVLQGARLTAWELMQDHIPVTLITDNM
                     AGALMRQGKIQLCVVGADRIAANGDVANKIGTYSVAVLARAHNIPFYVAAPYSTIDLT
                     TQSGAEIPIEERNPLEVTAIHGSHPIAPPGVAVLNPAFDVTPSGLITGIITERGVFKP
                     GELAAHFRS"
     gene            complement(5351..6412)
                     /locus_tag="PKK81_06310"
     CDS             complement(5351..6412)
                     /locus_tag="PKK81_06310"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV32520.1"
                     /translation="MIPHSKPSLGQDDIRAATEVLRSGLIAGGPVVGQFERGMAAYLG
                     VQGGVAVSSGTVALELALRAMGVGHGDNVILPSYVCPAPWLAVQRVGAQARIVDIDPV
                     TYNLDPYKVRKARTSRTRAVIVPHLFGLPADLTVLESLGIPLIEDCAQTLGAMEQGRA
                     VGSVGLLTVCSFYATKLLCTGEGGMVLSNDEALLERVRALREYDQAPSLNAAAFNCKM
                     TDLQAAIGVTQLNQLGEFLEKRAALAAVYRDSLPAELFTPPSVPAGRTHVYYRFVVRL
                     QKGLQSSDEFTAYLSRMAHRGLQCRKPVFRPLHRYLELADFPASDEADDVAISLPIYP
                     SLSDDDVKLASQILQEELR"
     gene            complement(6609..7790)
                     /locus_tag="PKK81_06315"
     CDS             complement(6609..7790)
                     /locus_tag="PKK81_06315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV32521.1"
                     /translation="MTKRLLFLAPAPPSDRQGGGALRMLHLLRFLGSRFQVDLIAPAL
                     DGAEDAQRLLKDVCAETMFVPQQGPGFLDRIGQVSPYVKDRALAAVVRERLASGQYGA
                     VHLEKPAMIPYLPPHVSVPVVLDVWSYSSTSPLRILRGVGGATGRSRQLVRLIKVGIF
                     DRFRWPMTHCVTVVSDEDRIRCERAHPGQRVLVVPNGVDCRTILPKPDHAATSPLLLF
                     TGDMGAEPNVEAALFLATEVFPAIRRDFPKAELRLVGRNPDPRVRRLAGSGIEVTGAV
                     PDMQLHLRAATMYVAPLCTGTGSRTKILEAMAAGLPIITTSVGIEGMKVQPGRDVLVA
                     DQPVDMIESIHTLLMSQPDRERFGHAARHMAEIWYDWSRCLWPLEPLYQNLLIPKAVA
                     C"
     gene            complement(8224..10755)
                     /locus_tag="PKK81_06320"
     CDS             complement(8224..10755)
                     /locus_tag="PKK81_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNV32522.1"
                     /translation="MLPTLTIIFVLGLVLGSYLTYFPLSIAIVLLACTAGCVWLERQH
                     RLTSQQSNVLLACLFGGCLHWTLCASFTPHVLLQDSARALPVRLTGTIVEAVRHAPGR
                     LTAMVQVTGSDDPQLPIPFHLRLTWRDPDRDLHRGLQIRTRIHVHAPSGTLNPRGFDY
                     AAYLDALGVDAVGSVSGAGVIEVLDPDREAPLHLFASLIEEWRGRVRAAAETLPQPSR
                     GLFLSLTIGEQGFLAPEVREWFMTTGTVHILSISGSHLGLIALLSFALVRKACLLLPS
                     ILLLSLSRWLPATRLAALLTLVPVLAYTVLAGAETATIRSAIMIAVALWTVWLGSPHY
                     LLHALAAAAGLTLLVHPAALFDISFQLSYVSVWALALALSRETPVDELPLPKESPAGR
                     MVYWFRESLRLTALVTLATVPLVACYFNQVSWMGLFANLLMVPFVGFIFLPTSLLSAV
                     WVIVTHSSTLPGAAFIDSLGQGLIASTHVLAGLPGAEWFVAAPTIPMMLLFYVLGWAL
                     LAGWPAHAAPLLRRSMASGLACILLWWLWSPRPFGGEEMVRVTFLDVGQGDSAVIELP
                     DGTVTLIDGGATYERFDMGRSVVAPFLWNRGIRRIDHVIGTHPQLDHVGGLAWILAHF
                     QVEKFWTNGAERQEEFWRRIERAMVQRKLEATVATEGRLISEGPACRMVVLNPQPRPE
                     SSISAKSESLNNLSVVTELVCREQRVLFTGDIEREALTRLTHSGNSAHIALLKVPHHG
                     AKSSLERRWLETIRPAVGVVSAGRRNPYGHPAGEVLAAYQAVETQVWRTDRDGAIWAD
                     LDLTRQELSMHSTREWILQPALPSADIWSVEQDNLRRLWRRWNWT"
     gene            complement(10782..12131)
                     /gene="glmM"
                     /locus_tag="PKK81_06325"
     CDS             complement(10782..12131)
                     /gene="glmM"
                     /locus_tag="PKK81_06325"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNV32523.1"
                     /translation="MRKLFGTDGVRGVANLEPMTSEIAMQLGRAAAHIFMRRAGRHQV
                     VIGKDTRLSGYMLESALTSGICSMGVDVLLVGPLPTPAIAFLTRSLRADAGVVISASH
                     NPYQDNGIKFFSNEGFKLPDELEARIEQLIVSDEIKHLRPTADAIGKAYRIDDAEGRY
                     IEFVKRSLPRDLDFQGIKLVVDCANGAAYKVAPAVFRELGAEIEVIANTPDGMNINDG
                     CGAVHPERLQEAVRRHGAHIGIALDGDADRAIFVCEQGKIIDGDHVMAALGLDSHAQG
                     QLARATVVGTVMSNFGLEIAMKKAGIQLMRTPVGDRYLMERMLADGYNFGGEQSGHFI
                     FLDHNTTGDGLISALQILSLMKRTGKPLSELAKAMTAVPQILLNVKVKHKPDLNQIPD
                     IQQAIKSAEVTLNGSGRVLVRYSGTESLLRIMVEGERDSTIREVADHLAEIVRARIG"
     gene            complement(12124..12933)
                     /gene="folP"
                     /locus_tag="PKK81_06330"
     CDS             complement(12124..12933)
                     /gene="folP"
                     /locus_tag="PKK81_06330"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNV32524.1"
                     /translation="MGVLNVTPDSFSDGGAYLTVEHALQHARQMQAEGADIIDIGAES
                     SRPGAQPIDETEELRRLLPVLEAVHDAVPLPISVDTTKAAVARRAIRAGATIVNDISA
                     LRGDPLMGSLVAETGAAVVLMHMQGTPQSMQQAPRYGHVVEEVLAFLRERVQVAIAHG
                     IKGSQIVLDPGFGFGKLQEHNLQLLAEFDAFTKLGYPVLAGVSRKQFIGNLTQQPVQE
                     RGYGTAGAVAVAVLKGARIIRVHDVRSMRDTVSVVSAISCHARSHVGVSNA"
     gene            complement(13213..>14935)
                     /gene="ftsH"
                     /locus_tag="PKK81_06335"
     CDS             complement(13213..>14935)
                     /gene="ftsH"
                     /locus_tag="PKK81_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNV32525.1"
                     /translation="PEDEVIFSDFMAKLDKGEVMKVTIKANHISAILKDQSRIRTYTA
                     EYPELVKHLREKDVQIEARPPDESPWYITFLVTWGPFILFLGLWFFLMRQMQIGGNKA
                     LSFGKSRARMLTEERKKVTFSDVAGIEEAKEEVLEIIEFLKDPRKFQKLGGRIPKGVL
                     IVGPPGTGKTLLAKAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKHAPCI
                     IFIDEIDAVGRLRGAGLGGGHDEREQTLNQLLVEMDGFDTTEGVILIAATNRPDVLDP
                     ALLRPGRFDRQVVVNRPDLRGRSEILKVHTKKVPLAADVELEKIARGTPGFSGADLEN
                     LVNEAALWAARWNKKEVELIDFEMAKDKVLMGAERKSMVLSDEEKRTTAYHEAGHALM
                     AKLLPGTDPVHKVTIIPRGRALGVTMQLPTDDRHNYSKDFLYNNLAILMGGRVAEELV
                     LHDVTTGAGNDLERATDLARKMVCEWGMSEKLGPLTFGRKEEEVFLGREMGSKRDFSE
                     QVALEIDHEVRRLVTENYERAKRILTDNMTSLKALAEALLEKEVLDALEIDQILIQGS
                     SSQTVPA"
BASE COUNT         3144 a         4718 c         4260 g         2813 t
ORIGIN      
        1 gatcccaagg tcaccgccaa catcgcaacc ttgatgagaa gtgagcgcag cattaggacg
       61 atggtttccg tgcaagatgg attgcgtggc catggacgtc ctccatggcc tccatgaggc
      121 cctcggccag ggtcgggtgc gcgtgaatca tctccgccac tcgcgagact gtggcgccaa
      181 gatgcatggc cagcgcggct tcatgaacga gatcggctgc gtgcgccccg agaatgtgta
      241 cacccagaat acggtcgctc tccgcatcgg ccaccacctt cagcaatcct tgaatgtccc
      301 cggtggcctg cgcttttccc aacccgccat accggaagcg cccgaccttc accgactgtt
      361 gcggatcttt cccggctgcc acgcaccggt cacgggcctg ttgctccgtg agccccaccc
      421 gcccgatttc aggtaacgta aagatgcccg tcgggatgac atcgtagtcg atcgtccggg
      481 gacggcccat gaaattctcg acggccacct ttccctgcgc cgacgcgaca tgcgccagca
      541 tcgccttgcc gaccacatcg ccgatcgcat agacgccggg cacattcgtc tccatccggt
      601 cgttgaccac gatttcgccg cgcgtcccga cctgtactcc tgccttctcc aatccaatgc
      661 cacgtgaatt gaacccccgc cctaccgaca ccagcacctg ctcaacattg agcgacaggc
      721 cgtcgcgaag atgggcggtg acgagatccg gctgacgaac gatctggtcg accgtgacgc
      781 cggtccggat gtccacgccg cgcttcttca gctcgcgctc catcatctgg ctgatctctt
      841 catcctccag cggcaacagg cgtggcacga gttcgaccag cgtcacctgc gtacccagcc
      901 cgctgtacag ggaggcgaac tcacagccct ccacgccccc gccgacgatc aacaggctca
      961 cgggaatcct gctgaggtcc aacgcctgct tgctggtgat gatttgagtc ccgtcgatag
     1021 gaaacaacgg gagattcggc caggacgagc cggtcgcaat gatcacgccg tcggccacga
     1081 ctcgggtttc ggtgccatcc ggtttcgtca cgcggatcgt acgcgcatca agcaactcac
     1141 ccgtgccttc cacgtgttcg atattccacg tcttgaataa cgtggcgatg cctttcacca
     1201 gcgtggagac aactttattt tttctggcca ccatgacggc gggatcgtag gtgacaggcc
     1261 cgctcagctg gataccgaag tccttggcct tcttcgcctt atcgccgagc tccacgaccg
     1321 acagcaacgc cttgctcggg atacagcccc aattcaggca gactccgccc agagcctgat
     1381 tttcgatgac ggtgacacgc gcgccgagtt gagccgcgcg aatcgccgcc acatacccgc
     1441 cgggaccggc gccaagaatt gcaaggtggt tcacgacgcg cctaatgttt ctgtttgctc
     1501 agaaacgttt cgtactgggc ggccgtcatc aactgatcca cttcagccgg cgctgtgagg
     1561 tcgatgaccg ccatccatcc cttgccgtac ggatcggaat tgaccacctc aggatgatct
     1621 ttgagatcgc cattgatctt cgcaacggtt ccgctgaccg gggtatagat cgtggacgtg
     1681 gttttggtcg attccacttc gccgatctgt tggccggcgg tgactttggc gcctaccttc
     1741 ggaaggtcga taaacacaat gtcgcccagc gcatcctggg caaaatgact gatgccgact
     1801 gtcgcctgcg ctccttcagc acggacccat tcatgttcct ggtggtaacg aagattggac
     1861 ggtatcatga ctctcctcgt gacggccgct gcacgagcgg cctacgtgaa taaaagcgca
     1921 cgggaaccgg gttgcgcgat cgtaaaagct gcgaaaaact ttgtcaagga atcaccacgg
     1981 gcgcgtccgg aacaaacaca aggtgacgaa tgtcgggcgt gcggagttca tccgcctgct
     2041 gcgcgagccc cgtgaacatg ccacccccgt gctccgccgg ggtgcggagc aacacccatc
     2101 cctgcggaag ggtttgcggc tcgcccttct ccgactgaat cacatcgcga ctccatccct
     2161 tgaacgcacc gccaaacgat cggacgtccg aagcctcgcg ggacgccccg cctaacagca
     2221 aatcaaaccc acgacgacgc gcctccgcgg catcttcccc gtagttcacc agggcggacg
     2281 tcggattcga caaggcctcc ggatgaattt cgatcaccat ccggaagcgc gggacggctt
     2341 tctgcaagta tttcttgaag cgggccagcg tatcgagttc ctgacgcgcc ttccacccga
     2401 cccagcgcca gagggtcttg tcggaattcg gagccggctc gttcggacga agtgccatgc
     2461 gatcacgaag ggcctgggcc acctcggagg aaggctgatg gacctgcgtt tcgaactggc
     2521 gcaacacgcc gtcgctcacc tcataggcaa aactattctc ggcgcggctg cggaacacca
     2581 ggccatcgac accggtcttc agcagatccg ccagcagctg cgccatttgt tgctgaacgg
     2641 ccggagccag gagatcgaac tgcgtccagg gatgcacctt gcgccgcgtc ggatcgtaga
     2701 gcagcatctt ggactccggt cgatggtcgg acagcggcgc atggtacaga tcgagcacgg
     2761 catagacgga aatccccagc tcatgggcct gcggcaccat aaccccatac cagtccgtga
     2821 gcacgggccc acgcgacgtg gcaaacaacg ctccaccggg cagagtcggc agcgggctgc
     2881 tcgtgggtgg gctcgtgggg atcagcggcc tggtaaagga cgactccaga ctggcatcca
     2941 tcaggatggc actgatgccc tgcgcgctca gctgcctgat ccaggcttca acctgggaca
     3001 tcggcggcag attgctgaag gagacataca acgcggtatg accggatctc gcgccggccg
     3061 gcgaagccgg ttggttgcgt agagcatcga ttcgatgctg ggcgcgttcg gcgtaggaat
     3121 tttgcgcgga aaggggcgcg ccctccggac cggccaacgt ccggtactct ttgatcgccg
     3181 ccgtcacttc acccatttgt tcatacgcct gccccagatc ccaatgggcc tccagcgcgc
     3241 gacgggattt cggatagttc gtgagatatc gttcgtagag atgaatggcg gcggaatacc
     3301 gtccatcgga gaacgcggtg gaggcttggt accagaggct ggtctccgcc gcgactgccg
     3361 gggtttccgt cggaggcacg acagcaggca tcgaggtcgg cggcgacccg gcacaaccag
     3421 ccacgaaaac catgagcagg ataaacgaaa gaagtggcga cccggctcga cggcccggca
     3481 aggaccgtcg agcatagatc acataagact tggctgcgct caacgtgcgc ctacccgacc
     3541 aactccattt cggcaaagaa atacccgatc tcgaacttgg ccgtctccgg cgcatccgaa
     3601 ccgtgcaccg cgttgaattc aatattggcc ccatgggccg cacggattgt cccctgctcg
     3661 gccttggccg gatcggttgc gcccatcagc tcgcgattct tcttgatggc attgtcgcct
     3721 tgcagcacca gcaccacaca ggggccggac gacatgaacg tacagaggct gtcgaagaac
     3781 ggacgcgcct tgtgcacggc ataaaatccc tgcgccgtgg ctttcgacat atgcatcagc
     3841 cgcatcgcca caggccgcaa tcccgctttt tcataccgat tgataatgtc gccgatcgcg
     3901 tttttcttca cggcatccgg tttcacaatg gcaagcgttc gttcactcat ggatcgtcag
     3961 gctccttcag ctaaaaaatc gacattcatt gaggcgtcag acacagtctc gcggcattat
     4021 aaagaagcgg cggaagggct ggcaaggtaa aggactacta gaaatgccgg ataaccattc
     4081 gcagatccga ccacccactc acgttcagcg acgcaagata caggcgcagc ccgcctctta
     4141 cgatcggaaa tgcgcagcca gttcccccgg cttgaacacg ccccgctcgg tgatgatacc
     4201 ggtaatcaac ccggacgggg tgacgtcgaa ggcagggttg agcaccgcca cgccgggcgg
     4261 agcgatcggg tggctgccgt gaatggcagt gacttccagc ggattgcgct cttcgatggg
     4321 aatctcggcg cccgattgcg tcgtgagatc gatggtggag tacggcgctg ccacatagaa
     4381 gggaatgttg tgcgcccgcg cgaggacggc cacggaatac gtgccgatct tattggccac
     4441 gtcgccgttg gcggcgatcc gatccgctcc gaccacgcag agctgaatct tcccctggcg
     4501 catgagcgca ccggccatgt tgtccgtgat gagcgtgaca ggaatgtgat cctgcatcaa
     4561 ctcccatgcc gtcagccgcg caccttgtaa taccggcctg gtttcatcgg caatgacctg
     4621 gatctgcttc ccctgctccc acgccgcccg aatgacaccc agggccgttc catacccggc
     4681 ggtcgcaagg gcgccggcgt tgcagtgcgt cagaatcgtc tgaccactct ggatcaacgc
     4741 ggccccatgc tgccccatgg ccttgcagag ggcgatatct tcatccagga tcgtctgcga
     4801 ttctgtgatg agctcggcct tgatccctgc gaccgggtga ttattgagcg ccgccagctt
     4861 ctgcttcatc cggccgatgg cccaaaacag attgacggcc gtcgggcgcg atgccgccag
     4921 atggtcgcag atctccccca cctgcgtggc aaagtcgcgg tactcggtgg ccgtcactgc
     4981 ctgcgccccc agcgccacac ccatggcggc cgtgacaccg atcgctggcg cgccacggac
     5041 tttcaagtcc cggatagcct gcgccaccgc gcgataatcg cgacagtcga ggaattcgac
     5101 gtgcatcggg aggcggcttt gatccagcag acggaccgct ccgtctttcc actcgacggt
     5161 gggcaccata ggcatgtcct ccggttgcgt aagggggaag gaatttgccg tcatctcagg
     5221 acgaccgaaa aatcgtgcca tcatgacgat cagcagggcg cgggtcaagg gtatgatggc
     5281 agggtgccca accatacgcg gtgcgggacc acgcgggtcg gtcgagagag cgatcaatac
     5341 gaaggcggcg ttaccggagc tcttcctgca ggatctgcga tgccagcttc acatcatcgt
     5401 cactcaacga tggataaatc ggcagcgaaa tggcgacatc atcggcctcg tcactggcag
     5461 gaaaatcagc caactcgaga taccgatgca agggccggaa gacgggcttg cggcactgca
     5521 gaccgcgatg cgccatacgc gagagatagg ccgtgaactc atcggacgac tgcagccctt
     5581 tctgcaaccg cacgacaaat cgatagtaga catgggttcg tcctgccgga accgagggcg
     5641 gagtgaagag ttcggcaggc aggctgtcgc gatagaccgc agccaacgct gctcgcttct
     5701 cgagaaattc tcccagctgg ttgagctgcg tgacgccgat cgcggcttgc agatccgtca
     5761 tcttgcagtt gaacgccgcc gcattgagtg acggcgcttg atcgtactcg cgaagtgccc
     5821 gcacccgctc cagcaacgcc tcatcgttcg acagcaccat gccgccctcg ccggtgcaga
     5881 gcagtttcgt ggcgtagaac gagcagaccg tgagcagacc gacgctgccg actgcgcggc
     5941 cctgttccat agcaccgagg gtctgcgcac aatcttcgat cagtggaatc ccgagggatt
     6001 ccaacaccgt cagatcagcc ggtaacccga acaggtgcgg cacgatcacc gcccgagtgc
     6061 gtgacgtgcg ggccttgcgg actttgtacg gatccagatt gtaggtaacc ggatcgatat
     6121 cgacgatcct ggcttgtgca ccgactcgct ggaccgccag ccagggcgcc ggacagacat
     6181 aactgggcag gatgacattg tcgccatgcc cgactcccat ggcacgcaat gccaactcca
     6241 gggccaccgt gcctgagctg accgccaccc cgccctgcac cccgagatac gcggccatac
     6301 cacgctcgaa ttgcccgacg acgggtcccc cggcgatcag accggatcgg agcacctcgg
     6361 tcgccgcacg aatgtcgtct tgtcccaggg aaggttttga atgcggaatc atcgtcatcg
     6421 cgtcaggagc cgcttgtcgt gccgggccag cagggagcag tcggagcctg aaatcgtgcc
     6481 ggcaggaaat tttacgtgga ggctccggca caacacaaca gattcctgtc gtgccgtgcc
     6541 gaactggggt tatagcgaga tcgtagagct gtcaccggat gtgattcgca gccgcccggt
     6601 ggcagcgctc aacaggccac ggccttcgga atcagaagat tctgatagag cggctccaac
     6661 ggccagagac agcggctcca gtcataccag atctccgcca tatggcgcgc cgcatggccg
     6721 aaccgctcgc gatcgggctg gctcatcagc aaggtgtgga tcgactcaat catgtcgacg
     6781 ggctgatcgg ccaccaacac atccctcccc ggttgtacct tcatcccttc gatcccgact
     6841 gacgtggtga tgatcggaag gcccgccgcc atggcctcca gtatttttgt ccgggacccg
     6901 gtgccggtac aaagaggcgc gacatacatc gtcgcggctc gcagatgaag ttgcatgtcg
     6961 ggaaccgcgc cggtcacctc gatgccggag cctgccaagc ggcgcacgcg ggggtccggg
     7021 ttcctcccga ccaatcgcaa ttccgccttg ggaaaatccc gtcgaatcgc aggaaacact
     7081 tccgtggcca ggaagagcgc ggcttcgaca ttcggttccg cccccatatc acctgtgaac
     7141 agcaacagag gcgacgtagc ggcatgatcc ggcttgggca ggatcgtccg gcaatcgacg
     7201 ccgttgggaa ccaccagcac ccgttggccc gggtgggcac gttcgcaacg gattcgatcc
     7261 tcgtccgaca cgaccgtcac acaatgtgtc atgggccagc ggaaccggtc gaagatcccc
     7321 accttgatga gccggaccag ttgtctcgac cgaccggtag ctccgcctac gcccctgagt
     7381 atccgcaacg gactcgtcga gctgtaggac cagacatcca gcaccaccgg caccgacaca
     7441 tggggcggga gatagggaat catcgcgggc ttttctaagt gcacggcccc gtattggccg
     7501 ctcgccaggc gttcacggac gacggcagcc aacgcccgat ctttgacata gggactcacc
     7561 tggccgattc gatcgaggaa accgggccct tgctgcggaa caaacatcgt ctccgcgcag
     7621 acatccttca acagtcgttg cgcatcctcc gcgccgtcga gcgccggagc aatcagatcc
     7681 acctgaaagc gactccccag gaaccgcaac aggtgcaaca tgcgcaaggc tcccccaccc
     7741 tgccggtcgg agggcggcgc aggagccaga aacaagagac gcttcgtcat gatcttgctg
     7801 ctttttttcc cagaatcatc cggatcaatc ccccagtctg atgtaccgcc ggccggggac
     7861 cgcacggacc atgcttttgg ctgtatcgcc tgactgcttc agcccatcga gtcgagtctt
     7921 tgctgcttcc ggtatccgac tcaagaatac ggatgccatt cgacctgctg tggcctgtga
     7981 tagacagcat gaccggacag tgcagcacgt ctgcatgtgc tatgccctgc cgatgctctg
     8041 ggggctctta cacaatattg acgacttacg cggtataggt atcctgcctg tcggcaaaat
     8101 tgacagcctg ctgccatccg agacggctaa atctgcacag gagggagatc gttgagcgat
     8161 gggaagatgt tcggtgacgg cacggagagc atgaacggtg ggccggcaac gagcaggctg
     8221 gggctacgtc caattccaac ggcgccataa gcggcgcaga ttgtcctgct ccacggacca
     8281 tatgtccgcc gatggcagcg ccggttgcag tatccattct ctggtgctgt gcatggagag
     8341 ttcctgtcga gtgagatcga gatcggccca gatggcacca tcccggtccg tcctccagac
     8401 ctgcgtttcc accgcctggt aggccgccaa gacttctccg gccggatgac catagggatt
     8461 gcgccgacca gcggatacga ccccgacggc cggcctgatc gtttcaagcc accttcgctc
     8521 cagcgaactc ttcgccccat gatgcggaac cttcaagagg gcaatgtgag cggaattccc
     8581 ggaatgggtg agacgggtca aggcttcccg ttcgatatct cccgtaaaca ggacacgctg
     8641 ctcgcgacag accaattcag tgacgaccga caggttgttc agcgactcgc tctttgcgga
     8701 gatcgacgac tccggcctgg gctgaggatt gagcaccacc atgcggcagg caggcccttc
     8761 ggaaatcaat cggccttccg tcgccaccgt ggcttcgagc ttccgctgca ccatcgctcg
     8821 ctcgatcctg cgccagaatt cttcctgccg ctccgccccg ttggtccaga atttctctac
     8881 ctgaaaatga gccaggatcc aggccagccc tccgacgtga tccaattgcg ggtgtgtccc
     8941 gatgacatga tcgatgcgtc gaatccctcg attccacaga aaaggcgcca ccacactccg
     9001 ccccatgtca aaccgctcat aggtcgcgcc tccatcgatc agcgtgaccg tcccatcggg
     9061 caattcaatg accgcactat caccctgccc cacgtcgagg aaggtcacgc gcaccatctc
     9121 ctcaccaccg aagggacgtg gagaccaaag ccaccagagc aggatacaag caaggcccga
     9181 ggccatggac cttctgagca gcggcgcagc atgggccggc caccccgcca acagcgccca
     9241 ccccagcaca tagaacagca gcatcatggg aatcgtcggt gcggccacga accattccgc
     9301 gcccggcaaa ccggccagca catgtgtcga agcaatcagc ccttgcccca acgaatcgat
     9361 gaacgcagcg ccagggagcg tgctgctgtg cgtcacaatc acccagacgg ccgacaacaa
     9421 actggtcggc aagaagatga aaccgacgaa gggcaccatg agaagattcg cgaataaacc
     9481 catccatgac acttgattga agtaacaggc caccaggggc acggtggcaa gcgtcaccaa
     9541 cgccgtcagt cgcagcgact cgcgaaacca gtacaccatc cgtccggcag gggattcctt
     9601 cggaagcggc aattcatcga caggcgtctc gcgcgacagc gccagggcca gcgcccagac
     9661 cgagacgtaa gacagttgaa aggaaatatc gaacagcgcc gcaggatgga ccaacagcgt
     9721 cagaccggcg gcggcagcga gtgcgtgaag gagatagtgc ggtgagccga gccatacggt
     9781 ccagagcgca acggcgatca tgatggcact acgaatcgtg gcggtctccg ccccggccag
     9841 caccgtatag gccagcactg gaaccagtgt cagcaacgcg gccagtctcg tcgccgggag
     9901 ccaccgcgag aggctcagca gaagtatgga cggcagcaac aggcacgcct tcctgaccaa
     9961 tgcgaacgag agcaaggcga tgagaccgag atgcgagccg gagatcgaga gaatgtgcac
    10021 cgtcccggtc gtcatgaacc attcacggac ttccggtgca agaaacccct gttcaccgat
    10081 ggtcagactg agaaacaggc cccgactggg ctggggaagc gtctcggcgg cggctctgac
    10141 ccggccgcgc cattcttcga tgagggaggc gaacaggtga agcggcgcct cccggtctgg
    10201 atcaagcacc tcgatgacgc cggctcctga gaccgatccg acggcatcga cacccaacgc
    10261 atccagatag gccgcatagt cgaatcctct cggattcaga gttcccgagg gtgcatggac
    10321 atggatgcga gtacgaatct gcaacccgcg gtggagatcg cggtccggat ctcgccacgt
    10381 aagacgcaag tgaaacggta tcggcaactg ggggtcgtcg ctgcccgtca cctgcaccat
    10441 agccgtgaga cggcccggcg catggcgaac ggcctccaca atcgttccgg tcaaccggac
    10501 aggaagcgcc cgggcactat cctgcagcag cacgtgcgga gtgaacgatg cacacagggt
    10561 ccaatgcaga catccgccga acagacaggc caggagcacg ttgctctgtt gtgacgtgag
    10621 gcgatgctga cgctccagcc acacgcagcc ggcagtacaa gccagcagga cgatcgcgat
    10681 gctgagtgga aaatacgtga ggtaggaacc gagaacgagg ccgagcacaa agatgatcgt
    10741 aagagtcggt aacatccggc gcctacggca tcggcccgat gctatccgat gcgggcacgc
    10801 acgatctccg caagatgatc ggcgacctca cggatggtgg aatcacgttc gccttcgacc
    10861 atgatgcgga gcagcgattc ggtgccggaa taccgcacaa gcacacggcc gctcccgttc
    10921 agggtcactt cggctgattt aatggcctgc tggatatccg ggatttggtt gagatccggc
    10981 ttatgcttca ctttcacgtt caacagaatc tgcgggacgg cagtcatcgc cttcgccaat
    11041 tcagacagcg gcttcccggt acgtttcatc agcgagagaa tctgcaacgc cgagatcaag
    11101 ccatcgcccg tggtgttgtg gtccaggaaa ataaagtggc cggattgttc tcctccgaag
    11161 ttgtatccgt cggccagcat ccgctccatg agataccggt cccccacggg cgtgcgcatc
    11221 agctggattc cggccttctt catggcaatt tccagcccga aattactcat gacggttccc
    11281 accacggtcg cgcgggcaag ttggccctgc gcgtgcgaat cgagtcccag cgcggccatg
    11341 acatggtcgc catcgatgat ttttccctgt tcacagacaa aaatggcccg gtcggcgtcc
    11401 ccgtccagcg caataccgat atgggcgcca tgccgacgca cggcctcctg caggcgctcc
    11461 ggatgcacgg ctccgcagcc gtcattgatg ttcattccat ccggagtatt tgcgatcact
    11521 tcaatttctg cgccgagttc gcggaaaacg gctggagcga ccttatacgc ggcaccgttg
    11581 gcacagtcca ccaccagctt gatcccctga aaatcgagat ctctgggaag cgagcgtttg
    11641 acgaattcaa tataccggcc ttcggcatca tcgatacgat aggcctttcc gatcgcgtcc
    11701 gccgtcgggc gaagatgttt gatctcgtcg gaaacgatca actgttcgat gcgcgcctct
    11761 agctcatcag gcagcttgaa tccctcgttg gaaaaaaact tgatcccgtt gtcctgataa
    11821 ggattatgcg acgcagagat cacgactccg gcgtccgccc gcagacttcg cgtgaggaag
    11881 gcaatcgccg gggtgggcaa tggcccgact agaagtacgt cgactcccat ggaacagatc
    11941 ccggacgtca gcgccgattc caacatgtat cccgacaacc gcgtgtcctt tccgatgacc
    12001 acctggtgtc ggcctgcgcg ccgcatgaaa atatgggcgg ccgctcgccc caattgcatg
    12061 gcgatttcac tggtcatcgg ttcgaggttg gccaccccgc gaacaccgtc tgtgccgaac
    12121 aatttacgca ttggataccc ctacatgaga acgcgcgtga caggaaatgg ctgacacgac
    12181 ggatacggta tctcgcatcg atcgcacgtc gtggacacga atgatccggg cccctttaag
    12241 gacggctact gccaccgcgc cggcggtgcc gtacccccgt tcctgcaccg gctgttgagt
    12301 caggttcccg ataaactgtt tacgggagac ccctgccaga accggataac cgagcttcgt
    12361 gaaggcgtca aactcagcga gcaattgaag attatgctct tggagcttac caaaaccgaa
    12421 gccggggtca aggacgatct gacttccctt gatgccgtga gcgatcgcga cctgcacccg
    12481 ttcgcgcagg aatgcaagca cctcttccac aacatggccg taccgtggcg cctgctgcat
    12541 agattgcgga gtcccctgca tgtgcatcaa caccacggcc gcgccggttt cagccaccag
    12601 cgagcccatc aaggggtcac cgcgcaatgc gctaatgtca ttcacgatgg tggcaccggc
    12661 acgaatcgct cgacgggcca ccgcggcttt agtcgtatca acggaaatag gaagcggcac
    12721 tgcgtcatgg accgcttcca ggacaggaag gaggcgacgt aattcctctg tttcgtcgat
    12781 cggctgagcc cctggccttg aagactcagc gccgatgtcg atgatatcgg caccttcggc
    12841 ctgcatctgt ctggcgtgct gcagggcatg ctcgaccgtg agataggcac cgccgtctga
    12901 aaatgaatcg ggggtgacgt tgagcacccc catgaggagt ggacctgatt caaaactgaa
    12961 cgtccgagag ccagcagtta agaggagagg gcttgaggat gaggtcgtgg tcacaatcag
    13021 gcaggtcctt ctctcttccg gatcaggaac aggacatcag tctgcgacat aacgcacctg
    13081 cgggagggaa atcaaaccga cggcggtcat gtataggagc ggcttcccga cccgtggcac
    13141 gaattgtata ggaagcgccg tcgtcgcagg ggcaacacga atgaagcccc tgcgacaaca
    13201 gcgatacaga gcttaggcgg gaaccgtctg cgacgaggat ccttgaatga ggatctgatc
    13261 gatctccaac gcgtccagca cttctttttc caacaacgcc tcagcaagcg ccttcaggct
    13321 cgtcatatta tcggtcaaaa tgcgcttcgc acgttcataa ttttccgtaa ccagacgtct
    13381 gacttcgtga tcgatttcga gcgcaacctg ttcgctgaaa tcacgcttcg accccatctc
    13441 acggccgagg aatacttcct cttctttcct gccgaacgtc agaggtccta acttttcact
    13501 catgccccat tcacacacca tttttctggc gagatctgtg gcccgctcca agtcattccc
    13561 tgccccggtc gtcacatcgt gtagcaccaa ttcttcagcc acacgaccgc ccatgagaat
    13621 cgcgagattg ttgtacagaa aatccttcga gtaattatgg cgatcgtcgg tgggaagctg
    13681 catcgtcaca ccgagcgccc gcccacgggg gatgatcgtg accttgtgaa cgggatccgt
    13741 ccccggcagc aacttggcca tcagtgcgtg accggcttca tgataggccg tagtgcgctt
    13801 ctcttcgtcg ctgagcacca tgctcttgcg ctcggccccc atcagcacct tgtctttcgc
    13861 catttcgaag tcgatcagct cgacttcttt cttgttccag cgcgccgccc acagcgccgc
    13921 ttcgttgacc agattttcca ggtcggcgcc ggagaatccg ggcgtgcccc gcgcaatctt
    13981 ctccaattcc acgtcggcag ccagcggcac cttcttggtg tggactttca gaatctccga
    14041 gcggcctcgc aagtccggcc ggttgaccac gacctggcgg tcgaaacggc ccgggcgcag
    14101 caacgccgga tcaagcacgt caggacggtt cgtggcggca atcagaatga cgccttccgt
    14161 cgtgtcgaac ccatccatct cgaccagcag ctggttgagc gtctgctcgc gttcatcgtg
    14221 gccgccaccg agaccggcgc cacgcaaacg accgaccgca tcgatttcgt cgatgaagat
    14281 gatgcaggga gcatgtttct tcccctgctc gaagagatct cggactctgg aagcaccgac
    14341 tccgacaaac atttccacaa agtccgatcc gctgatactg aagaacggaa cgcctgcctc
    14401 gccggcaatg gccttcgcca ataacgtctt tccagttccc ggaggaccga caatcaggac
    14461 acctttcgga atgcgcccgc ccaatttttg aaattttctg ggatccttta agaactcaat
    14521 gatctcaagg acttcttcct tcgcttcttc aataccggcg acatcggaga acgtcacctt
    14581 tttgcgctct tcggtcagca tgcgcgcgcg actctttccg aacgagagtg ctttatttcc
    14641 cccgatctgc atctggcgca tgaggaagaa ccagagaccg aggaaaagga taaagggtcc
    14701 ccacgtcacc aggaaggtga tgtaccaggg gctctcgtca gggggccggg cttcgatctg
    14761 gacatccttc tcacgcaagt gcttgaccaa ttcaggatac tcggccgtgt aggtccggat
    14821 ccggctttgg tcctttaaaa ttgcgctgat gtggttggcc ttgatcgtca ccttcatgac
    14881 ctcgccctta tcgagcttgg ccatgaaatc gctgaatatg acttcatctt ccggc
//
LOCUS       DAOQRM010000055        14856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3098_length_14856_cov_13.624079, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000055 DAOQRM010000000
VERSION     DAOQRM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14856
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3098_length_14856_cov_13.624079"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PKK81_06340"
     CDS             <1..954
                     /locus_tag="PKK81_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNV32526.1"
                     /translation="RMLGYLPDELLGQDMHERIHYSFTGGTAYPRTACRIQETLERGT
                     GCKVDDEVYWRKDGTDFPVEYSSFPIIEREQIIGAVVVFLDTTARKRAEQQLTLSHDQ
                     LRKLTARLESVREEERILIAREIHDELGQALTGVKLDLCLLRDQISEVSPAMMKRLES
                     ISTLVDGTIQSVQRIATELRPVVLDQLGLIPAIEWQAHEFQARTGIQCTLDVYLRTIT
                     LSHAGSTAMFRIFQEILTNVARHAQASIVHITLQEQAGSLVLDVRDNGRGVTDAELAD
                     PHSLGLVGMRERALLLGGETIVTGSPGTGTAVRVRIPLGQP"
     gene            1033..1677
                     /locus_tag="PKK81_06345"
     CDS             1033..1677
                     /locus_tag="PKK81_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV32527.1"
                     /translation="MTRILVVDDHAVVRQGVKQILSEQFHGAVLGDAQNAEEMIAQVG
                     KHSWDIVILDVGMPGKSGLDALKELKQARPKLPVLVLSGYPEDQLALRMLKAGAAGYL
                     SKDSAPNELVQALRKILGGGKFVSAAVAELLALNLESDWEKPLHEQLSDREYQVMCLI
                     AGGKSLKDIADDLCVGVSTINTYRARILEKMQLKNNTELTHYAIEHRLVNRLIR"
     gene            2740..3066
                     /locus_tag="PKK81_06350"
     CDS             2740..3066
                     /locus_tag="PKK81_06350"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNV32528.1"
                     /translation="MAQRRLRSIEDSVRIPSRSTTEMETSARQRSGPESPPQQADNEA
                     TTEEIRTRIEKLAYELYQQRGRQDGHDRQDWLEAERLTLTEPTRARHDRSDRLPTSVQ
                     ASRVGR"
     gene            3221..3511
                     /locus_tag="PKK81_06355"
     CDS             3221..3511
                     /locus_tag="PKK81_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32529.1"
                     /translation="MRAREGLQLLFSGQHEYVFSYGPSLSYLKVVLSTVALNLFSTHG
                     LEPVRLATHVAEWALLMGQVCGTVRPSGDLVPSSCYEYCRRQAVTHSGVSPR"
     gene            3533..>4269
                     /locus_tag="PKK81_06360"
     CDS             3533..>4269
                     /locus_tag="PKK81_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV32530.1"
                     /translation="MIHERGSGSALITIAIVSQNQLLRLGLQAVIAPHAHIRLIGEAT
                     NMLQAVSIVTQEKPQLLIIELQPGMDILGLVRKVKASLSTIRIIALHGIEDSRAGLEA
                     LSSGIDGMVLTTQPGVVLLAVIEYLCRRPANVARCHPCDTSALETTGSAGGFEDRIPV
                     LPNWSEDLTKREHDIITLIGEGLSNKDIACRLSICSTTVRHHLTSIFGKLDVHSRQQL
                     LIRAHKYGLVKLHANGTAARAHLVAGS"
     assembly_gap    4270..4369
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4370..4971)
                     /locus_tag="PKK81_06365"
     CDS             complement(<4370..4971)
                     /locus_tag="PKK81_06365"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV32531.1"
                     /translation="MKHRFVSLSHPCPSTSRSVRGLIVWLLAVMLVAVSGHATSSWAA
                     QTPTEVVERTMKDMLYILTELKDASRSAQRQWEIEQVVRRAFNYEEMAERSLGETGIK
                     SNAAERRQFVRLFVQVLRDDLADHLRDYSAAQVVYLAEGNDADGAQVMIAPAGQEVDT
                     RIEFQVVRRSGAWLVNDISIDGASIMAKYQAQFTRILREGS"
     gene            5174..6424
                     /locus_tag="PKK81_06370"
     CDS             5174..6424
                     /locus_tag="PKK81_06370"
                     /inference="COORDINATES: protein motif:HMM:NF023301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase domain-containing
                     protein"
                     /protein_id="HNV32532.1"
                     /translation="MSLPRMPLLLLSLSIPALFSGCETTDKMLGAAERAVGSSTGRTV
                     LDIVGGKDPAEIARRRVENYGRDPQALLRDLRSIQRDFQALMAALTGEVGKKWGTKEV
                     KLPEQKKYVKYTQNYRSRAIVDFDAGNILVETLDEKDPRASLKNAVVTTLLTPNDPRS
                     VDLFTDKEITLTSDKEPYLLGLVLDQAGKPVRTPAEAEQFADTLLSKSTTTRKVEQEE
                     GPKTAHMVNISMVTNFSHKQAEKYRAVVGQFAERYQISPSLVFAIIRTESNFNPFAVS
                     SAPAYGLMQLVPTSGGRDAYRKAKGEDKAPSRDYLFDPDNNIELGTAYLNVLTYAQLD
                     DVTDLVSREYCVISAYNTGAGNVFKTFSKDQRNALQQINGLQPAALYDRLRTGLPYQE
                     TRDYLAKVVGFRKQFVSLGDTAAK"
     gene            6620..8098
                     /locus_tag="PKK81_06375"
     CDS             6620..8098
                     /locus_tag="PKK81_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32533.1"
                     /translation="MTVEIPPLVDYPNHLARMHILADGGHSPWLRQYYDIHWDLLPNL
                     SMDLVVPPLTRIMSVEQAGKMFIALTFALLAGGTMALHAALHRRWSPWPLLAFFFLYN
                     SVFLWGFLNYLFGLGLALFACALWVRLRTRSALLVVPLFSLTAVMLLFAHLFAFGSLA
                     LIVSTYELSQWWRLRDEAIHPANAACWKAAPALIIPLIILTLTPTFKVAPEDYPLWLR
                     GSSPPPLISFLSPVTKLEALKGTIRTEHRLLDRVTVLMLVGLVGIGLVRRRCSILPSM
                     FLPLGATALAAMAMPNTIGTTAVVDIRMPIVFVLLTVASSDWREISRAWFLPVALALS
                     TVFAVRMGYITEHWQNTAHHYRQFKQILDQLPEGARLLSAIKMASYDNWSPAEGQIPE
                     PMPMVNLSCWGVIRRSVFVSNLFAAPGQQPVRLTPAVHALLTVEEFLFRAAPIPWDQL
                     RAQYDYVVVRRTQRLRPPPPADFVPFGSGDEFQFYRTGRVQP"
     gene            complement(8128..9222)
                     /locus_tag="PKK81_06380"
     CDS             complement(8128..9222)
                     /locus_tag="PKK81_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV32534.1"
                     /translation="MQGPVLSIIVPTFNERDNIPRLVGALDACLVGVEWELIVVDDDS
                     PDQTAAVVRAQACANHRVRCLHRIGRRGLSSACIEGMLSSSAPVVAVMDGDLQHDERL
                     LPDMLKVLLQGGYDIVVGSRYMAGGSIGEWDGMRALTSRWATRLSRPLVPAGLTDPMS
                     GFFAMRREVFERLVRRTSGMGFKLLLDVLASSPHPLRFIELPYEFRSRQVGESKLDSQ
                     VAWDYLMLLLDKSIGRFVPVRLVTFCFVGGSGVLIHFSLLWVLYRWLAVPFAWGQGIA
                     TLLAMTSNFILNNIITYRDQRLRGWQWLRGWLSFVLVCSLGAVGNVGVASYLFTQQFH
                     WGLSALAGILVGTVWNYAVTSSYTWKVKAR"
     gene            complement(9359..10084)
                     /locus_tag="PKK81_06385"
     CDS             complement(9359..10084)
                     /locus_tag="PKK81_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNV32535.1"
                     /translation="MDKALYPYLLFWSIGLLCFLAEWRFPARPIAYRSVFWRDLIALG
                     LYNVSFLAVVQATDRIPIPNYMPAVLYNLPTVVKLALFYIVEDFGLYWVHRLMHTKPV
                     WRIHRWHHSPTSLYWLSGIRATIPHIALFNLTYIIALPLLHEASAWAFQVIMVEHIVR
                     NNWMHLNVTWRSSWLEWVFVTPRYHQIHHSTDPAHQRTNLGALLTIWDRLFGTYYNPD
                     DVRGELSFGLAERVPPVRLVIGL"
     gene            10615..11106
                     /locus_tag="PKK81_06390"
     CDS             10615..11106
                     /locus_tag="PKK81_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32536.1"
                     /translation="MKRHIATYSAIVLACSWALLGPAPTQAAADNIEPVTWSNSQKSS
                     AWAEELLGQVVTYQTLAEKSLIPGNFEAYVEQMRKVRELYRTGNRRATYDGVNQLMVM
                     LEARVGGIDAHSADALWDFCYRVTPDEYHARDRHIRAKGADEVKKHEDFMRDMEERAS
                     MAF"
     gene            11222..12925
                     /locus_tag="PKK81_06395"
     CDS             11222..12925
                     /locus_tag="PKK81_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNV32537.1"
                     /translation="MLRSTLTRTVLLVCVILLESTGATLVLPPVWAESERAGYGPASP
                     GLSSSPVRLRFEPDDAVSSPLGSSFDLQHVWMRADLRVRPEWRNGVCFGGGPPIAGAC
                     NSLDQFGSGTGAHSGRHAGDFFIQQWARIGLGYDPSPNVNFYVELQDSATWGGNGDPT
                     GRNQVGDASTHNCGIQLAGQCRLGIRAGYMLVRNIAGIDGFSAKIGRQYVVFGNQRLF
                     GHFDWANTGYSHDGIMLSYATAAFDTKLGWFRNSETDLGQASPGGSLTPNLLTCNPEA
                     NSSATCNPALAQHATDAGSDVDLVVFYSQLRSIPRMIVEPYYVLYSNRLPERANPGQY
                     LPKSASQLRHMVGLRIEFRDGNWDFTHEAAYQFGRSADGFDFDNRRNLRINAWASGTW
                     LGYTWYTHHWKPRVAIGFDYASGDGDSNCVPPGGNLARSCGGNANTFENFFPTNFLHV
                     GYMLNGAWRNSVQPQVNVQARPTARDHIELWALRKYLASARDNWYRGSQGPLIFSASD
                     NRTTHIGDELDIAWSHMFADGTVSLSIIYGHFFSGSYIKHQLGTAADQDWGIVQLWTN
                     F"
     gene            12932..14485
                     /locus_tag="PKK81_06400"
     CDS             12932..14485
                     /locus_tag="PKK81_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV32538.1"
                     /translation="MQRATLNFETLLEVTNALNSQRDIDSLWRVIADQIRKVIPWDRA
                     GITLYESQTDSFRFYAVITNMATPALAHDSVIPRKGSAVGWVHDNRRLHIREDLQKEQ
                     VFLEDRYYVQEGLGRMINLPLLVREHCLGTLNIGSVQTGHLDQDDCKFLQQVATQIAY
                     AIDHVLAYEQIQQLSDRLRRENEYLAEEVKASRNLRLVVGTSLSFRKVVDLLKAVAPT
                     DTTVLLLGETGTGKEVLAQALHDLSLRSHKPFIRVNCAALPSGLIESELFGHERGAFT
                     GAQLRRAGRFELAHTGTLFLDEIGEMPLETQAKLLRVLQDGMVDRIGGTQPVAVDVRL
                     VAATNADLQAAVRQGTFRADLFYRLHIFPIALPPLRERREDIHLLAQHFLAQIGGKLK
                     RAHLAFDPQSMARLLTYNWPGNVRELQNVIERAVILSGSSQVTVDEMLLPVVQAVHSP
                     SSSQPVSLGDLERHHIIEVLERTKWRIYGEQGAAHLLGLNPETLRSRLRKLGLRRPAG
                     ISPEPTALP"
BASE COUNT         3258 a         4593 c         4003 g         2902 t
ORIGIN      
        1 cgcatgctgg gctatctccc ggacgagctc ctcggccaag atatgcatga gcgcatccat
       61 tattccttca cgggtggcac cgcatacccc cgtacggcct gccgtattca agagacgctc
      121 gagcgcggaa cagggtgcaa ggtggatgac gaagtgtact ggcgcaagga cgggacggat
      181 tttccggtgg agtactcttc gtttccgatc atcgaacgag agcagatcat cggagccgtg
      241 gtggtgtttc tcgacaccac cgcgcgcaaa cgggccgaac aacagctcac cctctcgcac
      301 gatcaactca gaaaactgac cgcgcggctc gaatcagttc gggaagagga gcgaattctg
      361 atcgcccgtg agatccacga cgaattgggg caagcgttga caggcgtgaa acttgacctc
      421 tgcctgttac gcgaccagat ctccgaagtc tctcctgcga tgatgaaaag gctggagtcg
      481 atttccacgc tggtcgatgg cacgatccag tcggttcaac gcatcgccac agaattacgc
      541 cctgtcgtcc tcgaccaact cggcctcatc cctgccatcg aatggcaggc ccatgaattt
      601 caagcacgca cagggattca atgcacgctc gacgtctatc tgcgcactat cacgttgtct
      661 catgccggtt cgacggccat gttcagaatt tttcaggaaa tcctgaccaa tgtggctcgc
      721 catgcccagg cgtcgatcgt ccatatcacc cttcaagagc aggccggcag ccttgtgctt
      781 gacgtgcgcg acaacgggcg cggcgtcacc gacgctgaat tggccgaccc gcattccctg
      841 ggcctggtgg gaatgcggga gcgagccctg cttctgggag gtgaaaccat cgtcacaggg
      901 agtcctggga caggcacggc ggtgagggtc cggattcctc ttggtcagcc atagtcggcc
      961 cggcgccgga acgcaacagg gggcactgcg cgttcaacct ctcaggcgcg atagcggatg
     1021 gaggaacagg ttgtgacaag aattctcgtt gtggatgatc acgccgtcgt ccgacagggc
     1081 gtcaaacaaa ttctcagcga acagttccat ggggccgtcc tcggcgacgc tcaaaacgcg
     1141 gaggagatga tcgcgcaggt agggaagcac agctgggata ttgtgattct cgatgtcggc
     1201 atgcccggga agagcggact cgatgcgctc aaagagctca aacaggcccg gccgaaactc
     1261 cccgtcctcg tcctgagcgg ttatccggaa gaccagctgg cattacgaat gttgaaagcc
     1321 ggcgcggccg gctacctgtc gaaagacagt gcgccgaacg aattagtgca agctctccgg
     1381 aagatcctcg gcggcggaaa attcgtcagt gccgcagtgg cggaactcct ggccctgaat
     1441 ctggagagtg attgggagaa accgctgcac gaacaattgt ctgatcgcga gtaccaggtc
     1501 atgtgtttaa tcgcgggggg gaaaagcttg aaagacatcg cggatgattt gtgcgtcggt
     1561 gtcagcacca tcaataccta tcgggcgcgc attctcgaaa aaatgcaact gaagaacaat
     1621 accgaattga cgcattacgc gatagaacat cgtctcgtca accggctcat caggtgagat
     1681 ccttcctctc tgtcatcatt gcctatccgt tcccgctcta caagctccct tcgtagcata
     1741 aacgtcgaca cgcgccgcac aggaaacggc gacgcgtaaa ttgcgtaaaa ggctatgctt
     1801 ttcggaacct gcttccggta cagatagcac tgacggtgca atctggttgc gagaccgttc
     1861 gcaccgtcat tcgcaagaga ggaccgggtg gcatggttgg gctggtccct ccttacaact
     1921 cagacgatga ccggtcagag ggggatgaca tgaccgagct ctatgagaca gaggccctca
     1981 ttatccgaga gctggaacag cagggcccct gcacattgga tgcactggcc tatagattcc
     2041 ccgcctgcac ctggaaccag atattcatcg cagtcgatgc cctgtcgcgg gaaggaacga
     2101 tctccctcca accgcgcgct gggtgccaat acctggtttc tctcgcgtct gtccacgcac
     2161 acaccttgca ccacttcgat cccgacaggc gtgtagcgga aacccggcag cacaccggcc
     2221 gttcgtacgg gagataagag ggaggtcgac acatgagcgc gacaatgaac agtaccaaac
     2281 agaccggaag atcccgtgtc atgatcgtcg atcctgaatg gcaattcgga ctcaaactgg
     2341 cggattgcct cgctaccaat gggtaccatg cagttctcgt tcgagacctt caatccatgc
     2401 tcaccgagct cggggagata cagccggagg ccatcctgct tagttcggac tcttgcgatg
     2461 agaaacgggg gaccgctgaa ctcgatactc ttcgagccct caacgtcctc tgcccacaag
     2521 cgccgatctt gaccgtcgtc gaaccgatgc gagacgcgtt gggtactctt tctccgccaa
     2581 tagacgctcg tccacgtcat caggctccgc tcgtgcacaa tcgcgtcgag gagttgctgc
     2641 gagcaaatct cggtattcca tgcgtacgcg catctctgac gtgatgcgct catcttggga
     2701 agaacaccac tcactcggac tcaacaggga ggttcagtca tggcacagcg acggttaagg
     2761 tcaatcgaag acagcgtccg gataccgagt cgatccacga cggagatgga gacctcggca
     2821 cgacaacggt cgggtcctga gagcccgccg cagcaggccg acaacgaagc gacgaccgag
     2881 gaaatcagga cgcgcatcga gaagttggcc tatgagctct atcagcaacg agggcggcag
     2941 gacgggcacg accgtcaaga ctggctggaa gccgaacgat tgacactgac ggaaccgacg
     3001 cgcgcgcgcc acgacagatc tgaccgcctc ccgacctctg tccaggcgtc acgcgtggga
     3061 cgatgagaag atcgcacacg gaagatcctg cacggagaac agtacgatgc tctgcccaac
     3121 caccaactgc ggccaactaa tggcatctga ttatcgtgca ggctatgacg cacgcactgg
     3181 tcttgaatgc ctccagtgtg gacactgcgg gcatcgtggg atgagggcac gagaagggct
     3241 gcagctgttg ttctccgggc agcatgaata tgtcttcagc tatggtccgt ccctctcgta
     3301 cctcaaggtc gtgctgtcga cggttgcgct gaatctgttc agcacccatg gcctggaacc
     3361 ggtccgactg gcaacccacg tcgccgaatg ggcactcctg atggggcagg tctgtggcac
     3421 ggttcgccct tcaggtgacc tcgtcccgtc cagttgttac gagtattgcc gacgacaggc
     3481 ggtgacccat tccggcgtct caccgcgcta aggctacgga ttcgaggact gattgattca
     3541 cgaacgcgga tcgggctctg ccctcattac catcgcgatc gtcagccaga atcagctcct
     3601 gcggcttgga ctgcaagccg tcatcgcacc gcacgcgcac atccggctga tcggagaggc
     3661 gacgaacatg ctgcaggcgg tgagcatcgt cacgcaagag aagccacagc tgctcatcat
     3721 tgagctgcag cccggcatgg acatcctcgg gctggttcga aaggtgaagg cttcgctttc
     3781 gaccatacga atcatcgccc tgcatggaat tgaagacagc cgagccgggc tggaagcctt
     3841 gtcgtccgga atagacggca tggtgttgac cacccagccg ggagtcgtgc tgcttgccgt
     3901 catcgagtat ctctgtcggc gtccggcgaa cgtggcgcgc tgtcatcctt gcgacacaag
     3961 cgcgttggag acaaccggct cagcaggcgg cttcgaggac cggatccccg ttctgccgaa
     4021 ttggtccgag gacctcacga aacgtgagca tgacatcatc accttgattg gagagggcct
     4081 gtccaacaaa gatatcgcct gccgcctcag catttgcagc acgaccgtgc gccatcacct
     4141 caccagcatt ttcggcaaac tcgatgtgca cagccgccag caactcctga ttcgagcgca
     4201 caaatacggc ctggtcaaac tccacgcgaa tgggacagcc gctcgcgcac acctcgtagc
     4261 gggttcggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aaccctcgcg
     4381 aaggatgcgc gtgaattgcg cctgatactt cgccatgatg ctggccccgt cgatgctgat
     4441 atcattgaca agccaagccc cggatcgccg aaccacttga aattcgatcc gtgtgtcgac
     4501 ttcctggccg gcaggcgcaa tcatgacttg tgccccgtcg gcatcgtttc cttcagcgag
     4561 atagaccact tgggccgcag aatagtctcg caggtggtcc gccagatcat cgcgtaacac
     4621 ctgcacaaag agtcgcacaa actgccggcg ctctgcagca ttcgacttga tcccggtctc
     4681 ccccagcgac cgttcggcca tttcttcata gttgaaggcc cgccggacca cctgctcgat
     4741 ttcccactga cgctgcgcag agcggctcgc atccttcaac tccgtcagga tatacaacat
     4801 gtccttcatc gtgcgctcga cgacctccgt aggcgtctgt gcagcccacg aagaggtggc
     4861 atgaccggat accgccacca acatcaccgc gagtagccac acgatcaacc cccggacgga
     4921 cctcgaagtc gagggacaag gatggctcag gctgacaaat ctgtgcttca tacgttctgc
     4981 cttccaataa cttctgccaa tgatcgttca tggtaccggc cgtcaaaacc gagcgtcaag
     5041 cacgtggata tacctgcgtt tcaatcgcca aaggatcagg tactgaagga gccggctatt
     5101 gcgccgaacg gtagcgacgc gccattgaga gattttattt tcctctctgc ggcaccttcg
     5161 gtatactcgc gccatgtctc ttccacgaat gcccctcctg ctgctgagtc tctccatccc
     5221 tgccctcttt tcaggctgcg aaacaaccga caaaatgctc ggcgccgccg aacgggctgt
     5281 cggcagcagc acaggaagaa ccgtcctgga tatcgtcggc ggcaaggatc cggctgaaat
     5341 cgccaggcgg cgtgtcgaga attatgggcg ggacccccaa gctttattgc gggaccttcg
     5401 gtcgatccag cgggacttcc aagcgctcat ggcggcgttg accggagaag tcgggaaaaa
     5461 gtggggcacc aaggaagtga aactgcccga gcagaagaaa tatgtgaaat acacgcagaa
     5521 ttaccgcagt cgcgccatcg tggatttcga tgcgggcaat atcctggtcg agacgcttga
     5581 cgagaaggat ccgcgggcca gcctgaagaa tgccgtcgtc accaccctcc tcactcccaa
     5641 cgatcctcgc agcgtcgatc tgtttacgga caaagaaatc actctgacca gtgacaaaga
     5701 gccgtacctt ttgggtctcg tgctggatca agccggcaag ccggtgcgca cacccgcgga
     5761 agcggaacag ttcgccgaca cactcctctc caagagcacg accacccgga aggtcgagca
     5821 agaagaggga ccgaaaaccg cccacatggt caacatctcg atggtcacga atttctcgca
     5881 caaacaggcc gagaaatacc gcgcggtcgt cggacagttt gcggagcgct accaaatcag
     5941 tcccagcctt gtctttgcca ttatccgcac cgagagtaac ttcaatccct ttgccgtcag
     6001 ttctgcgccg gcttacggat tgatgcaatt ggtccccacc agcggagggc gggatgcgta
     6061 ccgcaaggca aaaggtgaag acaaggcgcc ctcgcgcgac tacctcttcg atccggacaa
     6121 caacatcgaa ttgggcacgg cctatcttaa cgtgctgacg tacgcccaat tggacgatgt
     6181 caccgacctg gtctcgcgtg aatactgcgt gatctcagcc tataacaccg gcgccggcaa
     6241 cgtcttcaag acattctcca aagatcagcg gaacgcgttg cagcagatca atggattaca
     6301 accggccgca ctctatgacc gcctccgtac cggactcccc taccaggaga cccgcgacta
     6361 tctcgcgaag gtcgtcgggt ttcgaaagca atttgtgagt ctcggcgata ccgccgctaa
     6421 ataaccacgc accggttccg acaattctcc caaaccagtg atggttggat tcttgccttc
     6481 cggcgcccgc ttgtttacaa caagtcaggc aacgggtttt tcaaggagga ccaccgcgaa
     6541 ctcatcacct gacaagagca ctgcattcgg catcctcggc gccgccttcg ccctactgtg
     6601 catcgcgctg gtcccgttga tgacggttga gatcccgccg ctcgtagact atcccaatca
     6661 cctggcccgc atgcacatcc tagccgatgg cgggcattcc ccctggcttc gccaatacta
     6721 cgacatccac tgggacctgc ttcctaacct ttcgatggat ctggtggtgc cgccgcttac
     6781 ccggatcatg tcggttgagc aggcaggcaa gatgttcatc gcgctgacat tcgcactgct
     6841 ggcgggcggc acgatggcgc ttcacgctgc cctgcatcga cgctggtctc cctggccact
     6901 cctggcattt ttctttctct ataacagtgt cttcctgtgg ggcttcctca actatctctt
     6961 cgggctcgga ctggctctgt tcgcctgcgc cctgtgggtc aggctccgca ctcgttccgc
     7021 gctcctggtc gtgccgttgt tttcactcac cgccgtcatg ttgttattcg cccacctctt
     7081 cgcattcggc agcctcgcgc tgatcgtgtc gacctatgag ctctctcaat ggtggcgtct
     7141 gcgagacgag gccattcacc ctgccaacgc cgcctgctgg aaggccgctc cggcactcat
     7201 cattccactc atcatcctga cactgacccc gaccttcaaa gtggcccccg aagactatcc
     7261 cttgtggctc agaggatctt ccccgccccc actcatctcc tttctttctc ccgtaacaaa
     7321 actcgaagcc ctgaaaggga ccatccgaac cgaacaccgg ctcttagatc gcgtcaccgt
     7381 tctcatgctg gtcgggctgg tcggcatagg gttggtgcgg cgacgttgct ccatcctgcc
     7441 gtcgatgttc ctcccgctcg gcgcaaccgc gctggccgcc atggcgatgc ctaatacgat
     7501 cggcacaacc gcggtggtcg atatacgaat gccgatcgtc ttcgtcttac tcaccgtggc
     7561 gagttcggat tggcgcgaaa tcagtcgagc ttggtttctt ccggtggcac tggccctgag
     7621 cacggtattc gcggtgagga tgggctacat caccgagcac tggcagaaca cggcacacca
     7681 ctaccgacag ttcaaacaga tccttgatca gctgccggaa ggagcgcgcc tgctgtcggc
     7741 cattaaaatg gcctcctatg acaactggtc tccggcagaa ggccagatac ctgagcccat
     7801 gcccatggta aacctgtcct gctggggagt catccggcgg tcggtcttcg tctcgaatct
     7861 cttcgccgca ccgggccagc aaccggtccg actcacgccg gcagtccatg cactgctgac
     7921 ggtagaagaa ttcctctttc gtgcggcgcc gattccctgg gaccagctcc gcgcccaata
     7981 cgactacgtg gtcgtccgac gcacccaacg cctgcgcccc ccccctcctg ccgacttcgt
     8041 tccattcggc tcaggagacg agttccagtt ttatcgtacc ggaagggtac agccctaacg
     8101 cacggccgac agggcaatcc cgattgctca ccgagccttg actttccagg tgtaggacga
     8161 ggtgacggca taattccata ccgtccccac aagaatgcct gccagggcgg acaatcccca
     8221 atgaaattgc tgcgtgaaca gatacgacgc aacccccaca tttccgacag cgccaagact
     8281 acacaccagg acaaacgaca accaccctcg caaccactgc caccctcgta gccgctggtc
     8341 ccgataggta ataatgttgt tcaagatgaa attactcgtc atcgcaagca acgtggcaat
     8401 accctgcccc caggcaaagg gcaccgccaa ccagcgatac aacacccaca acagactgaa
     8461 gtggatcagg acgccggagc cccccacgaa gcaaaatgtc accaggcgca caggcacaaa
     8521 ccggccgatg gacttatcaa gcagcagcat gaggtagtcc cacgccacct gactgtcgag
     8581 cttgctctcc ccgacttgtc tcgacctgaa ttcatagggc agttctatga accgcaacgg
     8641 atgcggggac gaggccaaca catccagcag gagtttaaaa cccatcccgg acgtccgtct
     8701 caccagccgc tcgaacacct ctctgcgcat ggcaaaaaag ccgctcatcg ggtccgtcaa
     8761 tccggcaggc accagtgggc ggctcaaccg cgtagcccat cgactggtca aggcccgcat
     8821 gccatcccac tcgccgatgc tgccacccgc catgtaccgg ctcccgacca cgatgtcgta
     8881 cccaccctgc aataagacct tgagcatgtc ggggagcaat cgttcgtcgt gctgaaggtc
     8941 gccgtccatc acggccacaa ccggtgcgga actcgacaac atcccttcga tacaagctga
     9001 ggacaacccc cgacgtccaa tccgatggag acaacgcacc cgatggttgg cgcaggcctg
     9061 cgcgcgcacc actgcggcag tctggtccgg agagtcgtcg tcgaccacga taagttccca
     9121 ctctacccca accaggcagg cgtccagagc ccccacaagc cgggggatat tgtcccgttc
     9181 gttaaatgtc ggcacgatga tggacaggac aggcccttgc actactaccc tctcaatggc
     9241 tttcgcgaag catcagagac acctgccggc ccggaccgtg atcgacgcca ggctttgtcc
     9301 atcaggccga gaaccagctc gaagaagaca gcgaaacgct tgcgacgcaa tggtactctt
     9361 acaatccgat gaccagcctc accggcggga cccgttcggc aaggccaaac gacaattcac
     9421 ctctgacatc gtccggattg tagtaggttc cgaacagccg gtcccaaatc gtgagcaacg
     9481 cgcctagatt tgtccgttga tgagcaggat ctgtgctgtg atggatctga tggtatcgcg
     9541 gggtcacaaa cacccattcc agccaggacg acctccaggt gacattcaga tgcatccaat
     9601 tgttccgcac aatgtgctct accatgatca cctgaaatgc ccatgccgaa gcctcatgca
     9661 gtaacggaag ggcgatgata tacgtcaggt tgaacagagc gatatgcgga atggtggcgc
     9721 ggattccaga aagccagtac agggaggtgg gggaatggtg ccaacggtgg atgcgccaca
     9781 ccggcttggt atgcatgagc cgatgcaccc aatagagccc gaaatcttcg acgatataga
     9841 agagggccaa cttcacgacc gtgggcagat tgtacaggac cgcaggcata tagttcggga
     9901 tcggaatccg atccgtcgcc tgtaccacgg caaggaacga tacattgtac aaacctaacg
     9961 cgatcagatc ccgccagaat acagaccggt aggcaatcgg acgtgcagga aatcgccact
    10021 cggcgagaaa acacaggagc ccgatactcc agaacagcag atagggatac agcgccttat
    10081 ccataggcct ccgttcgcct cccaagcctc acgaggaacc tcctacgcgc cttattccga
    10141 aactgctccc cgccatgttc gccggcatcg gggcgggcgc ttgctcccct tcagtaccgc
    10201 cggattttac acttggccta ccctaccgaa aacctccgca gttgcgaaac cctttgtaac
    10261 tttccgcatc tccgctctcg gcggcgacct gccgcatgcc gatcgacatg cttcgtagac
    10321 gcccacatcg cagacaatcg atcggcacca ttgttttgcc cgttacaact catcgggcca
    10381 acgatcagca ccgttttatt tcacgccgtc accgtgaata gaaacggtgt actccgcacc
    10441 acatgagcgc ctgcatcaac ttttcgcact aattcaatgc gttggcaatc ttcgccctat
    10501 tcgcccgtaa aggcacaggt attgctctac ctacgagcaa atcgtggctc actaaccatg
    10561 cacaccatcc acccggcaaa tttctgccgg atagacctca aggaggagga caacatgaag
    10621 cgacacatcg caacctattc cgcaatcgtg ctggcctgca gctgggcctt actcggcccc
    10681 gctcccaccc aggcagccgc cgacaacatt gagccggtga cctggagcaa cagtcaaaaa
    10741 tcttccgcct gggcggaaga actgctgggc caggttgtga cctaccaaac actcgccgag
    10801 aagagcctga tcccaggaaa ttttgaggcc tatgtggagc agatgcgtaa agtgcgcgaa
    10861 ctgtaccgca cgggaaaccg gcgagccacc tacgacggcg tgaaccaact catggtcatg
    10921 ctggaagcca gggtcggagg aatcgatgcg cacagcgccg acgccttgtg ggacttctgc
    10981 tatcgcgtta ccccggatga ataccatgca cgcgatcgcc atatccgggc aaagggggcc
    11041 gatgaagtaa agaaacacga agactttatg cgggacatgg aagaaagagc gtcaatggct
    11101 ttctaagccc cattggcttc atcgaactgt ggaatctgcg gcgggccgac catccacccg
    11161 aggccgtcgg cccgccgccc cgcaccagtg aatggaggat tccgatgctc catgatcttc
    11221 gatgttacga tctacgctca cgagaaccgt cctgctggtg tgtgtcatcc tgcttgagag
    11281 cacaggcgca acccttgtct tgcctccggt gtgggcagaa agcgaacgcg ccggatatgg
    11341 gcctgcctca ccaggcttgt catcgtcacc cgtgcgcctt cggtttgaac cggatgacgc
    11401 ggtatcctcg cccctcgggt cgtccttcga tctccagcat gtctggatgc gcgcggatct
    11461 ccgggtgaga cctgaatggc gcaacggcgt ctgctttgga ggcggccccc cgatcgccgg
    11521 cgcctgcaac agtctcgatc aattcgggtc cggcacagga gctcatagcg gtcgtcatgc
    11581 cggcgatttc ttcatccagc agtgggcacg cattggcctg ggctatgatc cttcccccaa
    11641 tgtaaatttt tatgtcgagc tacaagactc cgccacatgg ggggggaatg gcgaccccac
    11701 cggaaggaat caagtgggtg acgcgagcac tcataattgt gggatccagc tagcgggaca
    11761 atgccgctta ggcattcgcg ccggctatat gctggtcagg aacatcgccg gtatcgatgg
    11821 attcagcgcc aagatcggcc ggcagtatgt cgtcttcggc aaccaacgac tcttcggcca
    11881 tttcgactgg gccaataccg gctattcgca cgacggcatc atgctgagtt atgcgaccgc
    11941 cgccttcgat acgaaactgg gctggtttcg aaattccgaa acggacttgg ggcaggcgag
    12001 cccgggtgga agccttacac caaacctgtt gacatgcaac ccggaggcaa actcctcggc
    12061 cacctgcaat ccggcccttg cgcaacatgc gaccgacgcg ggcagcgacg tggatctggt
    12121 ggtgttctac agtcaactcc gcagcatccc ccgcatgatc gtagagccct actatgtcct
    12181 gtactcgaac cgtcttccgg agcgggccaa tcccggccaa tacttaccca aatcggcctc
    12241 tcagttacgg cacatggtcg gcctccgcat tgagttccgg gacggcaatt gggatttcac
    12301 ccatgaagcg gcctatcaat tcggccgtag cgccgatgga ttcgatttcg acaaccggcg
    12361 caatctgcgt atcaatgcct gggcttccgg gacgtggctc ggctacacat ggtataccca
    12421 tcattggaag ccccgggtcg ccatcggatt cgactacgcc tccggagacg gcgacagcaa
    12481 ctgtgtcccc cccggcggaa acctggcacg cagttgcggc ggcaatgcca acacattcga
    12541 gaattttttc ccgaccaatt ttctgcacgt cggctacatg ctcaacggcg cctggcgcaa
    12601 tagcgtccag ccgcaggtga atgtccaggc tcgaccgacc gctcgagacc atatcgaact
    12661 ctgggcgctg cgcaagtact tggcaagcgc ccgagacaat tggtaccgtg gatcacaagg
    12721 gccgttgatt ttctccgcgt ccgacaaccg gacgactcac atcggcgatg aactcgacat
    12781 cgcatggagc catatgtttg cggacggtac ggtctcactg tcaatcatct acggccactt
    12841 cttttcgggc tcctatatca agcaccagct cggtaccgcc gcggaccagg actggggcat
    12901 tgtgcaactc tggacgaatt tttagacgac tatgcaacgc gccacactga attttgaaac
    12961 gctgctggaa gtaaccaacg ccctgaactc gcaacgggac atcgacagtc tctggcgcgt
    13021 catcgccgat caaattcgaa aagtgatccc ttgggatagg gccgggatta cgctctacga
    13081 atcacagacc gattcctttc gcttttatgc cgtcatcacc aacatggcga ctccggccct
    13141 ggcgcacgat agcgtcattc cacgcaaggg cagcgcagtc ggttgggtcc atgataaccg
    13201 tcgcctgcat atcagagagg atctccagaa agaacaagtc tttctggagg atcggtatta
    13261 cgtgcaggaa ggtttgggac gaatgattaa tctgcccttg ctcgtccggg aacattgcct
    13321 cggcacgttg aacatcggca gcgttcagac cgggcatctc gatcaggacg attgtaaatt
    13381 tctccagcaa gtcgccaccc agatcgcata cgcgatcgac cacgtattgg cgtacgagca
    13441 gatccagcag ctgagtgacc gcttgcgtcg agaaaatgag tatctcgcag aagaggtcaa
    13501 agccagccgc aatctgcgcc tggttgtggg aacctctttg tccttcagaa aggtggtcga
    13561 tcttttgaag gctgtcgccc cgacagacac gacggttctc ctcctcggag aaaccggcac
    13621 tggcaaagaa gtcctggccc aggctcttca cgatttgagc ctccgcagcc acaagccctt
    13681 tattcgcgtc aactgtgcag ccctgccatc aggactcatt gaaagtgagt tgtttggcca
    13741 cgagaggggc gcattcaccg gcgcgcagct acgaagagcc gggcgatttg aactggccca
    13801 caccggcacc ttgttcctgg acgagatcgg tgaaatgccg ttggaaaccc aagcgaagtt
    13861 gctccgagtg ttgcaagacg gtatggtcga tcgtatcgga ggaacccaac ccgtcgcagt
    13921 ggacgttcga ctggtcgctg ccaccaacgc cgacctccag gccgccgttc gacaggggac
    13981 gtttcgcgca gacctgttct accgccttca tattttcccc atcgccctgc cgcccttacg
    14041 ggagcggcgg gaagacattc acctgcttgc gcagcatttc ctggcgcaga tcgggggcaa
    14101 gctcaagcgg gcacatctcg cctttgatcc acaatcgatg gcgcggctcc tcacctacaa
    14161 ctggcccggc aacgtgcgtg agcttcaaaa tgtgatcgaa cgcgccgtta ttctttccgg
    14221 ctcctcccaa gtcaccgtag acgagatgtt gctcccggtc gtgcaggccg ttcacagccc
    14281 ctccagcagc cagccagtca gcttggggga tcttgagcgc caccacatca tcgaggtgct
    14341 ggaacggacg aaatggcgaa tctatggcga gcagggggcc gcgcatctgc tgggactcaa
    14401 ccccgaaacg ctgcggagcc gcctgagaaa acttgggctg cgacggccgg ctggcatatc
    14461 gccggaacct acggctctcc cctaacccat ggagattgct ggactgacgg tggaacctgc
    14521 ccttgtcgag ttccccctct gcgacgcagt atgacaacgc gcgctccagc cgaagccagg
    14581 tcatgcttcc gccaatcagc tcatttgacc ggactatgga aagcatgata aattgctgaa
    14641 ccgtacgagc cccgagcgaa atcccacgct tcgcgtttca gcccctatca cggcgcgatc
    14701 gcttccttaa cttacgctcc tcctacggcc catttccccc attcagcgga caaggaaatg
    14761 ggcaaagcag tggtccgaac cgcatgcccg acccactgca accagtttga tggcctggac
    14821 atcgccatcc tgttgttcgc cgaaaggagt gtacga
//
LOCUS       DAOQRM010000056        14541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3215_length_14541_cov_20.143656, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000056 DAOQRM010000000
VERSION     DAOQRM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3215_length_14541_cov_20.143656"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..750
                     /locus_tag="PKK81_06405"
     CDS             322..750
                     /locus_tag="PKK81_06405"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNV32539.1"
                     /translation="MKTATSMKNLGLGLCVVAAGFMVGGCNTTKATVDTTVNFFSSTS
                     PNGLFSADGMVLKEQKINLFAGVAYENLRQEAAAGGGQYVSALASLYEIPAARQGEFG
                     QVLQRKHADLFAAALEEDRTAHLKMVSALNRELVAASLLP"
     gene            complement(1007..1921)
                     /locus_tag="PKK81_06410"
     CDS             complement(1007..1921)
                     /locus_tag="PKK81_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32540.1"
                     /translation="MYRRWILSLVIAVCAVPGLALAEAPPHSVHWGAIAFPDHDPTLT
                     LSAALLDRFTEFDGEGRRYNDMRETMGLNFFTLSWTKPLAQLPGWNLNLTAGGGPTRD
                     GPSRFLQNDVVHKFRGLTEVPVGNKREANDFMLSGSLTRWFSLLGSNDAFFAGLGGAG
                     GSLYYEPYVQAGFRRLALFAPVPLLGDYLRVSALARYGRPFSGAAFHQVAPQSYMAQG
                     SVGLGNYRHWADSTPWEIELAVTVDSGLFVDHRGDALEERFVSVAVRYSAFTFETWND
                     LINQKDYGPTFGARLTLDLLYMYDRWFK"
     gene            2099..>2464
                     /locus_tag="PKK81_06415"
     CDS             2099..>2464
                     /locus_tag="PKK81_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HNV32541.1"
                     /translation="MPPKKKQTTTGTGSSTDDAAEFEKLGVFYLGRPYDLAAKQAKPG
                     WLLYDSKDLVTHAVCVGMTGSGKTGLCVGLLEEAAIDGIPAIVIDPKGDLANLLLTFP
                     QLRGEDFAPWINEDDARKKG"
     assembly_gap    2465..2564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2565..4749
                     /locus_tag="PKK81_06420"
     CDS             <2565..4749
                     /locus_tag="PKK81_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32542.1"
                     /translation="RGEDFAPWINEDDARKKGLSAADFAAQQATLWQKGLGDWGQSGE
                     RIKKLRDAADFAVYTPGSNAGLPVSILKSFAAPSAELLDDAELLRERVGTTVTSLLGL
                     IGVEADPIKSREHILLSSILDSAWRAGRDLDLPALIHQIQTPPMTKVGVLDVESFFPS
                     KERFALAMQMNNLLAAPGFAAWMEGEALDVGRMLYGPSGKPRIAIFSIAHLTDAERMF
                     FVSLLLNQTLGWVRGQSGTTSLRAILYMDEIFGYFPPVANPPSKAPLLTLLKQARAFG
                     LGVVLATQNPVDLDYKGLANTGTWFIGRLQTERDKARVMEGLEGAAASSGKKFDRRQM
                     EQILAGLGNRVFLLNNVHEDAPEVFQTRWTLSYLRGPLTRTQIKQLMDPIKRERPAEA
                     GQSTGTPVASTPAHGTPHAQRPMLPPEVPQYFVPLRGRQPEGHALVYQPMVLGVAQVR
                     VVDNKAAVDVTDPLTLLTPLTEGAVPVEWDHATIVDLAVADLERDPAGGAQFATLPAA
                     AGKAKQFDGWKNDLSDWLFRTQKVELFRSPSTKALSMPGEPERDFRVRLQQAGREQRD
                     QQSEVLRKKYSPKIVALQDRIRRAEQMVERQQAESRSSQIQAAISVGATILGAFLGRK
                     AISAGNIGKATTAIRGAGRAMKESKDVSQAEDNVAALQQQLAALEAEFKAEADALAAA
                     TDPLTEKLETLSLKPSKSNISIKLVALVWVPHWRAADGTSLPAWS"
     gene            4898..>8413
                     /locus_tag="PKK81_06425"
     CDS             4898..>8413
                     /locus_tag="PKK81_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV32543.1"
                     /translation="MATPAPPSDEIARIAALSRYRILDTLPDIAFDDLAHLAAAVCRA
                     PIAVITFIDRDRQWFKAAVGLEVTQTARSSGLCSHIIERGEPLVVEDAQDDPRFSSHV
                     LVASDPYVRFYCGVPVVTTDGYAVGTIAVMDPVPRRREEKECLALKRLARQAAALLEL
                     RMLRCEQPAVEERCVQDEEMVRLMAGSSRSTGLDFLRELVDTLAHSIGGAYAFCIRPL
                     SGQRAITIAGYGPDGPLDTLEYDLTGTPCEHVLNEGFCHYPGAVQASFPQDDLLRHLS
                     IESYMALTLPSPTGEPIGWIALLARAPLKDGRRTESLLRMAAGCAGSELGRHLAEQQL
                     RKSEERFRAAYRNATVGMSIADLNGRIEEVNQRLCAILGYSEAELLTRTFQSLTHPDD
                     LGQNLERIKKLVEGTADGDVFEKRYIRKDGEIVWAQVGLSVIRDATGRARHTLAMVQD
                     ITERKRTKEALYLAKYTIDHATEAIYWVGAGAELLDANDAACDMLGYTKDEFRRMTVH
                     DINPEFQADRWPAFWNDVQRRGRDFFEGHHRAKDGRLIPIEVNVMFIRYNQVECHCAF
                     VRDITERKQAEQALAHTQEKLWQALRASSTGLWDWNTETDEVFFSDEWAQQLGYSSQE
                     IDGRFEEWESRLHSDDHDRALAYVQNYLNDPIGPYEQEFRMRRKDGSYCWICARASFV
                     EESDGRKVRLLGSHTDLTERKLAETALRASEERFHITIEAINEGMWDWNIPTNVVYFS
                     PQWIRLLGYLPEEVTGSTAFFHSILHPDDVHRMTEILQAHLDGRTPLKELELRLRHKS
                     GEYRWYLDRGKVVARDEQGRPLRMVGTITDITERKQVERERAEALANLQTIMETVPDV
                     IFVLDLDGRLSKWNLRLETVTGYTCDELLGKPALEMVPATEVEQTSAAIRRALETGYA
                     ELEGHLLTKDGRPLRYHWNGAPFADLQGRVVGITGVGRDITERQQTEALLRSSEERFR
                     LVAQATNDILWDWDLLTGDHWWSPNACEKFGYDPHKEPNIDAWSGRLHPEDRERVLDL
                     VGHAIHTEISTLSAEYRFQLADGTYGYFLDRAHIVRDEAGVAVRMIGAMIDVTGPRRA
                     YASLEEAYRRFQAMSQELHTVESNERRRLSRELHDEVGQLLTSLKFDLTSVKRSVAGR
                     SAGVGVRGQERLARALDTTDQLFTRL"
     assembly_gap    8414..8513
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8514..9081
                     /locus_tag="PKK81_06430"
     CDS             <8514..9081
                     /locus_tag="PKK81_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32544.1"
                     /translation="QERLARALDTTDQLFTRLRRIVRALRPPVLEELGLKAGLEALIA
                     DVHARTRLRCALVFEQAPRRGARQPTLETAFYRSVQELLTNVIRHAQATTVSILVQQS
                     RREWRLTVTDDGVGFDVAGLSPLGRFGLRGIRERVEILAGHVEISSRVDSGTTVQVCI
                     PVGTTADGAAAAGVPSASASRRRKPVHE"
     gene            9074..9712
                     /locus_tag="PKK81_06435"
     CDS             9074..9712
                     /locus_tag="PKK81_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV32545.1"
                     /translation="MNKCRCLLADDHPIVRRGVRELLEEEQLSSEICEVRSGEEALAA
                     VRRQPWDLMILDIALPDKHGLEVLKETKLLQPRLPVLMLSLYPEKEFAMRAIKAGASG
                     YLTKQSAPSELLAAVMRVLQGGRYITAALAEQMASVIETGVDDSLHARLSDRELQVLR
                     LLGQGKSVSTIAEELRLSVKTISTYRARILEKLSCESTGELIRYAIEARLID"
     gene            9870..10550
                     /locus_tag="PKK81_06440"
     CDS             9870..10550
                     /locus_tag="PKK81_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV32546.1"
                     /translation="MDAQHLYRIVLIDDSRMARLGLREQLNRTAEFRIVGEAASIAEG
                     LELIERLNPDLVLLDISLPDGSGVQACCRLVAGRPDTRVLILSVYDDPAVIREAIAAG
                     AQGYVLKDTPVAMWRNAMRRAAGSGVVLGPQLIGLVLMEVREMANGLSTACMVDLSPQ
                     ERRLLPLVAEGRTNKEIAVALSLSDKTVKNYISNMYSKLGVTRRSQVATLYARTLPSG
                     GIPAEDCA"
     gene            10569..10952
                     /locus_tag="PKK81_06445"
     CDS             10569..10952
                     /locus_tag="PKK81_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV32547.1"
                     /translation="MALILVADDDEKVCRWLRTVLELEGYRVIEAADGRQALHTIQRQ
                     SPDLVILDVHLPVQDRLETILRVHSRHMPVKVLAISGQAVQGYDILKIAAIFGAHGTL
                     EKPFSVDRLLLGVRALIGPAHPRVA"
     gene            complement(11057..>14541)
                     /locus_tag="PKK81_06450"
     CDS             complement(11057..>14541)
                     /locus_tag="PKK81_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV32548.1"
                     /translation="LFGIYLRNLHLNWMVLLPLLMVPLLVPRWTIALAQLNTPGRTLP
                     VWLLQAVFMIGLGLAVMALIYLHLCRPTLREYRRNTRWQTLERQHWFLVACLGPLITS
                     VLFLTTAWAWFRNGGGTLEQLSLPHAILGGVFLHTGSWLFSVLALKRFKAFSPWLFWE
                     TAAVAATGALGGLFLRSILMKTPDQLVVATFAECFATFAVPGVLAIFLLTATVFIGLA
                     SRFTEEQDREWWGRTGSWVLIVMVIWSGLSAIVAFGPGLIAWTPKIAASLGGLSGLLT
                     LVLGFGSRTTAQQQEERSQTTVITDFAVRAAAPFFMLCVLIALSLGTSWQLDLFAHHY
                     EMHLNHLTGPMQTGLGIWPDPWGHDQVLHNTPLWMLFLFTVAVTSLGLLMSGLININR
                     FSLHAMYRNRLIRAYLGASRIQAERERSFNPFTGFDNLDNPAMGDVRFDDVRLARWMV
                     REVFSTDTRAQLDAFHALTEPTSAQLQAMIAHLTQELSRSLTNKALVTAAEQLRVGPA
                     CRAKIAAWQQPETAAGKEAITWLTTLFLNKLYDQSPRPLHLINIALNLVKGDNLAWQQ
                     RKAQSFTISALHSGSWNLGYRRSEEYGCNHYLAQPLSLGTALAISGAAASPNMGYHSS
                     SAVTFLLALFNIRLGWWLGNPGRAGADTYRKASPNVSVGPLLSEAFGLTDSCHPYVYL
                     SDGGHFENLGLYEMVLRRCHCILVVDAGCDPQLNFEDLGNAIRKIRIDLGIDIELNLD
                     QIKRATDAKTSSRHHAIGMIRYDKVDANATAGTLIYLKPSLTGNEPSDVQDYTARHPS
                     FPHEPTSDQFFDEAQFESYRRLGEHIAQQVLRPAIQRSEQDFSSLCHALRSYWLTTPP
                     GMRESFLGETDDLKDLERLLRDDPDLLRYDLEIYPELRSVFGIDPGAIAVNPRAALHT
                     CNLQIQLMEQVYLAVNLEEFHNHALNRGWMNLFRRWTSAETFRLFWPTLCGMYSQQFV
                     RFAEQHLNLSIDEVVVLEAMGPASDLSSLARDLSIEWDSVPDYAETFMQALHKPLPLE
                     EPTGDHTRRAAWHMRLKARPSPDDSAGPGEILAVVAATRLSVAGHRLALHGWVRPAYR
                     GLGLGHRLFRRAIEELTAAYSGHNLIVDLGPDNPAWAGTSIRNVGWLRFYEQLGFTRD
                     RSDPARFQMTRILR"
BASE COUNT         2869 a         3985 c         4687 g         2800 t
ORIGIN      
        1 ggcttcggaa tgatgatcgg tgccgtgttt tttgtcttta aggaatggtg atcgcggtca
       61 ttcgacagct ctttcaactt cttcctactg ttagacgagc cttcccagtt ccccccaatc
      121 ctttggcgcc tcgatcaaat ttttcctcgt agtctcggtc ctggcatctg acaggcgcca
      181 gcgttagccg ctttgctagc gcctttcttc cctctctcca gggtaatgac tagggtctcc
      241 ccgaatggtt atcggttaca acgtgagtag aaggctggat tgggaaatga tctcacgctg
      301 acagccccga gaaggaggcg tatgaagaca gcaacatcaa tgaaaaacct gggattgggc
      361 ctgtgtgttg tggcagccgg cttcatggtc gggggctgta acacaacgaa agctacggtc
      421 gacacgaccg tcaatttctt ctccagcacg agccccaatg ggctcttttc agcggacggc
      481 atggtgctga aagagcagaa gatcaatctc tttgcagggg tggcgtacga gaacctccgt
      541 caggaggctg cggctggtgg gggccagtac gtgagtgcct tggcctcgct gtacgaaatt
      601 ccggccgcca gacaagggga gttcgggcag gtgctgcagc gcaaacacgc cgacttattt
      661 gcggcggccc tggaggaaga tcgcacagcg catctgaaga tggtcagcgc tctgaatcgt
      721 gagctggtgg cagcgtcgct attaccataa tctgcggttg gtcgggagga atgtatggga
      781 acgatgggaa aaggatttat cctctggtta ctcggagttc cggcaagcct gctggtcctc
      841 ttatggtttc tgggcattct caagtaacca ggctatgcag aggagattgg cttgcgggcg
      901 gacaggttgt ctgtgcctga ggagtcagtg tgagactcct gagggcagga gcgcgggacc
      961 gaatccctcc tgttttgtcc gcccgaatgg ctgaggtcct ggtcttctac ttgaaccaac
     1021 ggtcgtacat atacagcaaa tccagcgtca gtctggcgcc gaaggtcggg ccgtagtctt
     1081 tctggttgat gagatcgttc caggtttcga acgtgaaggc cgagtagcgc accgcgactg
     1141 agacgaatcg ttcttccaac gcatcccccc gatgatctac gaacagacct gaatcgacgg
     1201 tcaccgccag ttcgatttcc catggagtcg agtcggccca gtgccgatag ttcccgaggc
     1261 cgacagagcc ctgagccatg taggactgtg gcgcgacctg gtgaaacgcg gcaccgctga
     1321 acggacggcc gtatcgcgcg agggctgaaa cgcgaaggta gtcgcctagc agcggtaccg
     1381 gagcgaagag ggccaaccgg cgaaatcctg cctgcacata gggttcataa tacaacgacc
     1441 caccggcgcc acccagaccg gcaaagaacg catcgttcga tcccaagaga ctgaaccagc
     1501 gggtcagcga cccgctgagc atgaagtcgt tcgcttcgcg tttgttcccg accgggactt
     1561 cggtgagccc tcgaaacttg tgcaccacat cgttttgaag aaaccggctc ggcccgtcgc
     1621 gcgtgggtcc gccgcctgcc gtgagattga gattccatcc cggcaattgg gccaggggct
     1681 tggtccagct caaggtgaag aaattcaacc ccatggtttc ccgcatatcg ttgtagcgcc
     1741 tgccttcccc gtcgaactcg gtaaaccgat ccaggagtgc ggcgctcaag gtcaaggtag
     1801 ggtcgtgatc ggggaaggcg atggcgcccc agtggacgct atgcgggggc gcttcggcca
     1861 gggcgagtcc cgggaccgcg cagacggcga tcacgaggga taagatccat cgacgataca
     1921 tcggggcgca tagtgcctgt gtcgatttca tttcgcaagt agtcggtacg taatcgcacc
     1981 gcaagatcgc accggtcgac tgtgcccgcg ggcttgcagt cgcggcctgt gcttactaga
     2041 atgcccgtcg aattcggtcg attcgaattg gtgctcttca tctccattgc ggggaattat
     2101 gccgccgaag aaaaaacaga ccacgaccgg taccggctca tcgacggacg acgcagccga
     2161 gttcgaaaag ctcggcgtgt tttatctcgg gcggccttat gatcttgccg ccaaacaggc
     2221 caagcccggc tggctgctct acgattcgaa ggatctggtc actcacgcgg tctgcgtcgg
     2281 tatgacggga agcggcaaga ccggtctctg tgtcggttta ctggaagaag cggccatcga
     2341 cggcatcccg gccatcgtca ttgatccgaa gggcgatctc gccaatcttc tgctcacctt
     2401 tcctcagctt cgcggcgaag atttcgcgcc gtggattaat gaagacgacg cccgcaagaa
     2461 gggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgcgg cgaagatttc
     2581 gcgccgtgga ttaatgaaga cgacgcccgc aagaagggct tgtccgccgc cgacttcgcc
     2641 gcgcagcagg cgaccctgtg gcagaagggg cttggcgact ggggccagag cggcgagcgt
     2701 atcaagaagt tgcgcgatgc ggcggacttc gcggtgtaca cacccggaag caacgccggt
     2761 cttccggtgt cgattctgaa atccttcgcc gctccatcgg cagaattgct cgacgatgcc
     2821 gaactcctcc gcgagcgcgt cggcacgacc gtgaccagcc tgttgggctt gatcggcgtc
     2881 gaggccgatc cgatcaagag ccgcgagcac attctgctat cgtcgattct cgacagcgcc
     2941 tggcgagcgg gacgggatct cgatctgccc gcgctcattc atcagatcca aacgcctccc
     3001 atgacgaagg tgggtgtgct cgatgtggag tcgttcttcc cctcgaagga acgattcgcc
     3061 ctggccatgc agatgaacaa cctgctggcc gcgccgggtt tcgccgcctg gatggaaggg
     3121 gaggcgttgg atgtcgggcg tatgctctac ggcccctcgg gcaaaccccg catcgccatt
     3181 ttttcgatcg cccatttgac cgatgccgag cgcatgttct ttgtctccct gcttctgaac
     3241 cagacgttgg ggtgggtccg cggccaatcc ggcaccacga gcctgcgggc tattctctat
     3301 atggatgaga ttttcggcta cttcccgccg gtcgccaatc cgccgtccaa agctcccttg
     3361 cttaccttgc tcaagcaggc gcgcgcgttc gggttgggtg tggtgctggc cacgcaaaac
     3421 ccggtcgatc ttgactacaa aggcctggcc aataccggta cctggttcat cggtcggttg
     3481 cagacggagc gcgacaaggc gcgcgtgatg gaagggttgg agggcgcggc ggccagcagc
     3541 ggcaagaaat tcgatcgccg acagatggag caaattcttg cggggcttgg gaaccgagtg
     3601 ttcctgctga ataatgtgca tgaagatgcg ccggaagttt tccagacccg atggacgctg
     3661 tcctatctgc gcgggccgct cacgcggaca caaatcaaac aattgatgga tcccattaag
     3721 cgggagagac cagccgaagc gggccagtcg acaggcacac cagtggcatc gacgcctgca
     3781 catgggacgc cccatgcgca acgccccatg ttgccgccgg aggtgccgca atactttgtc
     3841 ccgctacggg gacgccaacc ggaggggcac gcactcgtct atcaaccgat ggtcttgggg
     3901 gtggcacagg tacgggtggt ggacaacaag gctgcggtcg atgtgaccga ccccctcacc
     3961 ctactgactc cgctgaccga gggtgccgtg ccggtggaat gggatcatgc gacgatcgtc
     4021 gatttggccg tagcggatct ggagcgcgat cctgcaggcg gggctcagtt cgccacgctg
     4081 ccggctgccg ccggcaaggc caagcagttt gacggttgga agaacgactt gtcagactgg
     4141 ttattccgga cgcaaaaagt tgaactattc cgtagtccga gcacgaaggc gctttcgatg
     4201 cccggggagc cggagcgtga ctttcgtgtg cggctgcaac aggcgggacg ggagcagcgc
     4261 gatcaacaga gtgaggtatt gcgaaagaag tattcaccca agatcgtcgc attgcaggat
     4321 cgtatccggc gcgcagaaca aatggtggaa cggcagcagg ccgagtcccg cagcagtcag
     4381 atccaggcgg ccatctcggt cggcgccact atcctggggg cgtttctcgg gcggaaagca
     4441 atcagcgccg gcaatatcgg taaggcgacg acggcgatcc gcggcgccgg cagggcgatg
     4501 aaagagtcga aagacgtgag tcaggcggaa gacaacgtgg cggcgttgca gcaacagttg
     4561 gcggcgctcg aggccgagtt taaagcggaa gccgatgccc tggcggcggc gaccgatccg
     4621 ctcacggaaa aattggaaac cctgtcgttg aaacctagca aatcgaacat ctcgatcaag
     4681 ctcgtggctc tcgtttgggt gccccattgg cgcgcggccg atggaacgtc actccctgcc
     4741 tggtcatagc gtcgcccgca agtaggccaa cggagggaag cggcaggcgt cgcccccgtt
     4801 ccagatggcc ctattcgatg cggggcattt gtggtaagac tctgggcgaa atcggtacac
     4861 ccaaagccct cgattgttcg cgcgtgggac tgtacgtatg gcgacgcccg ccccgccttc
     4921 cgacgaaatt gcccgcatag ccgcgttgtc gcgctaccgg attctggaca cgctccccga
     4981 catcgccttc gatgacttgg cgcatctggc tgcggcggtg tgtcgtgctc cgatcgccgt
     5041 catcaccttc atcgaccggg atcgtcagtg gttcaaagct gccgttgggc tggaagttac
     5101 ccagaccgcc cgctcgtccg gactctgttc ccatatcatt gaacgcgggg aaccgttggt
     5161 ggtggaggat gcgcaggacg atccgcggtt ctcgtcccac gtattggtcg cgtccgaccc
     5221 atacgtcaga ttttattgcg gggtgccggt cgtcacgacg gatggctatg cggtcgggac
     5281 gatcgccgtg atggatcctg tgccgcgaag gcgggaggag aaggagtgtc tggcccttaa
     5341 acgattggct cgacaggcgg ccgcgctgct ggaacttcgg atgctgcggt gcgagcaacc
     5401 ggccgtggag gagcggtgcg tacaggacga agagatggtc cgcctcatgg ccgggagcag
     5461 tcgatctacc gggctggact ttctgcggga gcttgtcgac acgctggcgc actcgatcgg
     5521 cggggcatac gcgttctgta ttcgacccct gtcggggcag cgcgcgatca ccatcgccgg
     5581 atatgggccg gatggtccgc tggacacctt ggaatatgat ctcaccggga cgccctgcga
     5641 gcacgtcttg aacgaaggct tctgtcacta tccgggcgcc gtgcaagctt cgtttccgca
     5701 ggatgacctg cttcgacatc tttccattga gtcatacatg gccctgacgc ttccttcgcc
     5761 gaccggtgag cccatcggct ggattgccct gcttgccagg gcaccgttga aggacggcag
     5821 gaggacagag tcgctgctgc gcatggctgc cggttgcgcc ggtagtgagt taggacgcca
     5881 tctcgccgaa cagcagctca ggaagagtga ggagcggttc cgcgctgcgt atcggaatgc
     5941 gacggtcggg atgtcgatcg ccgacctgaa cggccgtatc gaggaagtca atcagcggct
     6001 ctgtgcgatc ctcgggtact cggaggcgga gctgttgacc cgtacgtttc aatcgctcac
     6061 gcaccccgac gacctgggcc agaatcttga acggatcaag aaactggtgg aagggacggc
     6121 cgacggcgac gtcttcgaga aacgctatat cagaaaagac ggagagatcg tgtgggcgca
     6181 agtgggactc tcggtcatac gggatgccac tggtcgcgca cgccatacgc tggcgatggt
     6241 gcaggacatc accgagcgca aacgaaccaa agaggcgttg tacctcgcca aatacacgat
     6301 cgatcacgcc acagaggcga tttactgggt aggggccggt gcggagctgc tggatgcgaa
     6361 cgatgccgcc tgcgacatgc tgggttacac gaaagacgag tttcgccgca tgacagtgca
     6421 tgacatcaat ccggaattcc aggccgacag gtggccggcc ttttggaacg atgtgcagcg
     6481 acggggcagg gatttctttg aggggcatca tcgggccaaa gacggccggc tcattccgat
     6541 cgaagtcaat gtgatgttta tccgttataa ccaggtggaa tgtcattgcg cattcgtgcg
     6601 ggatatcacc gagcggaaac aggcggaaca ggccctggcg catacgcagg agaaactctg
     6661 gcaagcgctt cgggcgtcca gcacgggact ctgggattgg aatacggaaa ccgatgaagt
     6721 gtttttttcg gacgaatggg cgcagcagct cgggtactca agccaggaaa tcgacggccg
     6781 gtttgaggaa tgggaatcgc ggctgcactc cgacgaccac gaccgagccc tggcctatgt
     6841 gcagaactat ctcaacgatc caatcggccc ctatgagcaa gagtttcgca tgcgccggaa
     6901 ggacggttcc tactgttgga tctgtgcacg ggcctccttt gtcgaagaat cggacgggag
     6961 gaaagtccgg cttctcgggt cgcataccga tctcaccgaa cgcaagctgg ccgaaacggc
     7021 cttgcgggcg agtgaggaac ggttccacat taccatcgaa gcgatcaatg aagggatgtg
     7081 ggactggaac attccgacga atgtcgtcta tttcagtcct cagtggatca ggcttctggg
     7141 atatctgccg gaggaggtca ccggatcgac agcgttcttc cattcgattc tgcatccgga
     7201 cgatgttcat cgcatgacgg agattctgca agcgcatctg gatggtcgta cgcccctgaa
     7261 ggaactcgaa ctgcgcttac gccacaagtc cggcgaatac cgatggtatc ttgatcgcgg
     7321 caaagtcgtg gcccgggatg agcagggacg tccgttgcgc atggtcggaa ccattaccga
     7381 tatcactgag cgcaaacagg tggagcgtga acgggcggaa gcattggcca atttgcagac
     7441 catcatggaa acggtgcccg atgttatctt tgtattggat ttggacgggc gtctgagcaa
     7501 gtggaatctt cgattggaaa cggtcaccgg ctatacgtgc gacgagctgt tgggcaagcc
     7561 cgccctcgag atggtgcccg ccacagaagt tgagcagacc agtgcggcta tccggcgggc
     7621 gcttgaaaca ggatatgcgg aattggaagg ccacctcctg acaaaagacg ggcgtcccct
     7681 tcgctaccat tggaacggag cgcccttcgc ggacttgcag ggtcgggtcg tcgggattac
     7741 cggggtgggt cgtgatatta ccgaacgaca gcagaccgag gccctactcc ggtccagtga
     7801 ggagcggttt cgcctcgtgg cgcaggccac caacgacatc ttgtgggatt gggacctgct
     7861 gacgggcgat cattggtgga gtcccaatgc ctgcgagaaa ttcgggtacg atcctcacaa
     7921 ggaaccgaac atcgacgcat ggagcggccg cttgcacccg gaagacaggg agcgggtgct
     7981 cgacctggtc ggccacgcca ttcacacaga gatcagcacc ctctccgccg aataccgctt
     8041 tcaactggcc gacggcacct atgggtactt tctggatcgc gcccatatcg tacgggacga
     8101 ggccggggtg gccgtacgaa tgatcggggc tatgatcgac gtcacgggac ctcggcgagc
     8161 ctatgcgtcc ctggaggaag cctatcgacg atttcaggcc atgtcgcagg aactgcacac
     8221 ggtggagtcc aacgaacgtc gccggctgtc gcgcgaactc cacgacgaag tcggccaatt
     8281 gctgacctcg ttgaagttcg atttgacgtc ggtgaagcga agcgtcgcgg gacgatctgc
     8341 gggcgtggga gtacgcgggc aggaacgtct cgctcgtgcg ttagacacga cggaccagtt
     8401 atttacgcgt ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcaggaa
     8521 cgtctcgctc gtgcgttaga cacgacggac cagttattta cgcgtctgcg tcgtattgtc
     8581 cgagccctcc gccctccggt cttggaggaa ctaggactga aggccggtct agaggctctg
     8641 atcgcagacg tgcacgccag gactcgtctg cgctgtgcac tggtgttcga gcaggcacca
     8701 cgccgtggcg cgcgtcagcc cacgttggag acggcgttct atcggagcgt ccaggaactg
     8761 ctgacgaatg tcatccgcca tgcgcaggcc acgaccgtgt ccattctggt tcagcagagt
     8821 cggcgggaat ggcgattgac ggtcaccgac gatggcgtgg gattcgatgt cgcgggtctc
     8881 tcccccttgg ggaggttcgg actgcgaggc atacgggaac gggtggagat tctcgccggt
     8941 catgtggaga tcagttcccg ggtcgattcc ggcaccacgg tccaggtgtg tattcctgtc
     9001 ggcaccacgg ctgacggggc tgctgcagcg ggggtgccat cagcctcggc atcgcgacgg
     9061 aggaaaccgg tgcatgaata agtgtcgctg tctgctcgca gacgatcatc cgatcgtacg
     9121 aaggggtgtc cgtgaactat tggaggagga gcaactctcc tccgagatct gcgaagtccg
     9181 ttccggggag gaggccttgg cggccgttcg gcgtcagccc tgggatctca tgattctgga
     9241 cattgccctt cccgacaagc acgggttgga ggtgttgaag gaaaccaaac tcctgcagcc
     9301 tcggctcccg gtgctcatgc tgagtctcta tccggaaaaa gagtttgcaa tgcgagcgat
     9361 caaagccgga gcgtcggggt atttgaccaa gcagagtgcg ccctccgagt tgctggcagc
     9421 cgtcatgcgg gtcttgcagg gaggccggta cattactgcg gcgttggccg agcaaatggc
     9481 gagcgtgatc gaaaccggcg tggacgattc cttgcatgcc agactgtcgg atcgcgaact
     9541 gcaagtgctg cgattgctgg gacaggggaa atcggtgtcg acgatcgccg aagaacttcg
     9601 cctcagcgtg aaaaccatca gtacctaccg ggcgaggatt ctcgaaaaac tgtcctgtga
     9661 gagcacgggt gaattgatcc gctacgcgat cgaagcgcgg ctcatcgact agccgcggcg
     9721 cttccgcgac ccgctctgag cgagccgtct ccgtcctgtc agccgaatac tgacaggtaa
     9781 atccaccttc tgcctatact cctgcgtgcg ccctccgccg agactatgct cgcaaaaatc
     9841 gccatgcgat tggcgagcta ggagcggcga tggacgcaca gcatttgtat cgcattgttt
     9901 tgattgatga tagccgcatg gcccgccttg gcttgaggga acaactgaat cgaacggcgg
     9961 agttccgtat tgtcggcgag gcggcgtcga ttgcggaggg tcttgagctg atcgaaaggc
    10021 tgaatccaga cctggtgctg ctggatatca gtttgccgga cgggtcgggt gtgcaggcct
    10081 gctgtcggct ggtcgccggc aggccggaca cgcgggtgct cattctgagc gtgtacgacg
    10141 accccgctgt catccgggag gcgatcgctg cgggcgctca ggggtatgtg ttgaaggata
    10201 cgccggtggc gatgtggcgg aatgcgatgc gacgggcggc cggtagtgga gtggtccttg
    10261 ggccgcagtt gatcggactg gtcttgatgg aagtgcggga gatggccaat gggctgtcga
    10321 cggcgtgcat ggtcgacttg tcgccgcaag agcgacgcct cttgcccttg gtcgcagagg
    10381 gcaggacgaa caaggagatt gccgtagccc tgtcgctctc cgataaaacg gtcaagaact
    10441 atatctccaa tatgtattcc aagttaggag ttacgcggcg gtcgcaggtg gcgacgctgt
    10501 atgcgaggac gcttccgagc ggggggattc ctgccgaaga ctgtgcctga tgcggaagcg
    10561 aggagccggt ggcgctgatt ctggttgcag acgacgatga gaaagtgtgt cggtggctgc
    10621 gaaccgtact cgaactggaa gggtatcggg tcatcgaagc cgcggatgga cggcaggctc
    10681 tccacaccat tcagcgtcag tctcccgatc tcgtcattct tgatgtccac ctgccggttc
    10741 aggacaggtt ggaaaccatt cttcgagtgc atagccggca catgcccgtg aaggtcttgg
    10801 ccatctccgg gcaagcggtg cagggctacg atattctcaa aatagcggcg atcttcggcg
    10861 cgcatggcac gctggaaaaa cccttcagtg tcgaccggtt gctgctcgga gtgcgggcgc
    10921 tcatcggacc cgcgcatccc cgtgttgcgt gacgctgtct gccgttcccc acatccctga
    10981 cagaccggtc aagcagggcc actgtgtaac gttgagaatg aattcgtcca tccttagccg
    11041 gccgggtcta cctgtgttac ctcaagatcc gggtcatctg gaagcgggcc ggatctgaac
    11101 gatcccgcgt aaacccgagt tgttcgtaga aacggagcca gccgacgttg cggatggacg
    11161 tgccggccca ggccggattg tccggcccca aatcgacgat cagattgtgc ccggagtacg
    11221 ccgccgttaa ttcttcgatg gcacgtctga acaggcggtg tcccaagccc aagcctcgat
    11281 aggccggtcg tacccaccca tgcagcgcga gccggtgtcc ggccaccgaa agacgggtcg
    11341 cggccacgac ggccaggatt tctcccggcc cggccgaatc gtccggtgag ggcctggctt
    11401 tcagtcgcat gtgccaagcc gctcgccgcg tgtggtcgcc ggtcggttcc tccaacggca
    11461 acggtttgtg gagcgcctgc atgaacgtct ccgcgtaatc cgggacggaa tcccactcaa
    11521 tcgacaggtc tctggcgagc gacgagagat cactggccgg ccccatcgct tccaacacga
    11581 ccacctcatc gatacttaaa ttcaagtgtt gctcggcgaa gcggacgaat tgttggctgt
    11641 acatgccgca gagcgtcggc cagaagagac ggaaggtttc ggctgaggtc cagcgccgga
    11701 acaggttcat ccagccgcga ttgagggcgt gattgtggaa ctcttcgagg ttcacggcca
    11761 ggtagacctg ttccatgagt tggatctgca gattgcaggt gtgcagcgcg gctcgcggat
    11821 tgaccgcgat ggccccggga tcgatcccga acaccgagcg gagttcagga tagatttcca
    11881 ggtcgtaacg aagcaggtcc ggatcgtcgc gcaggaggcg ttcgagatcc ttcaggtcgt
    11941 cggtttcgcc gaggaaggat tcacgcatgc ccggcggggt ggtcagccag taggagcgga
    12001 gtgcatggca gagcgaggag aagtcctgct cgcttcgctg aatggcgggg cgcagcacct
    12061 gttgagcgat atgttcgccg aggcggcggt aggactcgaa ctgggcttcg tcgaagaact
    12121 ggtccgaggt cggttcgtgg ggaaacgagg ggtgacgcgc ggtgtagtcc tggacgtccg
    12181 acggctcgtt cccggtcagc gagggtttga gatagatcag ggtgccggcg gtggcgttgg
    12241 catccacttt gtcgtagcga atcatgccga tggcgtggtg gcgactgctg gtcttggcgt
    12301 cggtggcccg cttgatctgg tcgagattca gttcgatgtc gattcccaga tcgatgcgga
    12361 tcttccggat ggcgttgccg agatcttcaa agttgagctg cgggtcacac ccggcatcca
    12421 ccacgaggat gcagtgacag cggcgcaaga ccatttcgta caagccgaga ttttcgaaat
    12481 gcccgccgtc ggagaggtag acgtagggat gacaggagtc cgtgaggccg aaggcttcgg
    12541 acagcaaggg gccgaccgag acgttgggtg acgctttgcg ataggtgtcg gcgcccgcac
    12601 ggcccgggtt tccgagccac cagcccaggc ggatgttgaa gagcgcgagg agaaacgtca
    12661 cggctgaaga tgagtgatag cccatgttgg ggctcgcggc ggcgcctgag atcgccagag
    12721 cggtcccgag agaaaggggt tgggcgaggt aatggttgca tccgtactcc tccgagcggc
    12781 gataccccag attccaactg ccgctgtgga gcgcgctgat cgtaaacgat tgggctttcc
    12841 gttgttgcca ggccaggttg tcgcccttga ccagattgag cgcgatgttg atgagatgca
    12901 gcgggcgcgg gctctggtcg tacaacttat tgagaaaaag tgtggtgagc caggtgatgg
    12961 cctcttttcc cgccgccgtt tccggttgtt gccaggcggc gatcttggcg cggcaggccg
    13021 gtccgacgcg caattgttcg gcggccgtga ccagcgcttt gttcgtgagc gaacgggaaa
    13081 gttcctgcgt caggtgtgcg atcatggcct gcagttgggc cgatgtcggc tccgtgagcg
    13141 catggaacgc atccagctgc gcgcgggtgt ccgttgaaaa cacctctctg accatccagc
    13201 gcgccagacg cacgtcgtcg aatcgcacgt cgcccatggc cgggttatcc agattgtcga
    13261 atccggtgaa gggattgaag ctccgttccc gttcggcctg aatgcgcgag gctccgagat
    13321 aggcgcggat gaggcggttg cgatacatcg catgcagcga gaaccggttg atgttgatca
    13381 gcccggacat gagcaggccc aatgacgtca cggcgacggt gaacaggaag agcatccaga
    13441 ggggggtgtt gtggaggacc tgatcgtgcc cccagggatc cggccagatt cctaatccgg
    13501 tctgcatagg ccccgtaaga tggttgaggt gcatctcgta gtgatgggcg aagaggtcta
    13561 attgccaact ggtgcccagt gacagtgcga tgaggacgca gagcatgaag aagggcgctg
    13621 ccgctcgtac cgcgaagtcg gtgatcacgg tggtctgtga ccgttcctcc tgctgttgcg
    13681 cggtggtgcg tgagccgaat ccaagcacga gggtcagcaa tccggagagt ccgccgaggg
    13741 aagcggcgat tttcggcgtc caggcgatga gaccgggacc gaaggcgacg atggcggaga
    13801 gccccgacca gatgaccatg acgatcaaca cccaggaacc ggtgcgcccc caccactcgc
    13861 gatcctgctc ttcggtgaac cggctggcca ggccgatgaa gacggtcgcc gtgagcaaaa
    13921 atatcgcgag gacgcccgga acggcgaagg tggcaaagca ctcggcaaat gtggcgacga
    13981 ccagctgatc gggagtcttc atcaggatag atcggagaaa caatccgccc agcgcgccgg
    14041 tggcggcgac ggcggcggtt tcccagaaca gccaggggga gaacgctttg aatcgtttca
    14101 gcgcgaggac ggaaaagagc cagctgcctg tgtggaggaa gacgccgccg agtatggcgt
    14161 gggggaggct gagttgttcc agcgtgccgc ctccgttgcg aaaccaggcc caggctgtgg
    14221 tgaggaagag caccgatgtg atcaaaggac cgaggcaggc gaccagaaac cagtgttggc
    14281 gttccaatgt ctgccaccgg gtattgcgcc ggtactcgcg gagcgtagga cgacagaggt
    14341 gcagatagat gagcgccatg acggccagcc cgagaccgat catgaacacg gcttggagaa
    14401 gccagacggg caaggtccgt ccgggggtat tcagctgggc cagggcgatg gtccagcgtg
    14461 gcacgagcag gggtaccatc agcaatggca gcagcaccat ccagttcagg tgcaggttgc
    14521 gcaagtaaat gccgaagagc g
//
LOCUS       DAOQRM010000057        14256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3312_length_14256_cov_16.636364, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000057 DAOQRM010000000
VERSION     DAOQRM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14256
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3312_length_14256_cov_16.636364"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKK81_06455"
     CDS             complement(<1..638)
                     /locus_tag="PKK81_06455"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNV32549.1"
                     /translation="MFSGIVEEMGAVSVLNKGLAGTRLTIIASTIMSDLTIGASVSVN
                     GTCLTAVARTDHDFSVDVSPETLAVTTLGGLTSGSPVNLERAMKLNERIGGHMVSGHV
                     DGIGAIRSRHQDGNALILEIEAPKEILRLCVQKGSITVDGISLTINDVTERSFVVSII
                     PHTAKVTTLGLKQVGDKLNLESDLIGKYVERLLQERGLLPPKPTPVIDTDYL"
     gene            complement(813..1988)
                     /locus_tag="PKK81_06460"
     CDS             complement(813..1988)
                     /locus_tag="PKK81_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV32550.1"
                     /translation="MTTSRSFFYLCTLGVFCFISYNLVRMPVLSLFAESLGAGPERIG
                     LIVSVSTITGVLLKLPSGALSDIYGRKMLLRIGVVAFGLPPFIYPFISDLNALTALRF
                     VHGLATAIFAPSALATVADLYKERRGAALGTYTACTQSGSLLGPFLGGWLAYTAGFST
                     AFVTAGVFGCIAILIFFSLHLDEPPPRVRERGLAPVMAEMGKGFLAVARNRKVLITSS
                     TDAAKMVANGALMAFLPLYGLSVGLNAGQVGLLFSVQAVTSFLSKPVMGRVSDRVGRQ
                     PLILAGLLICAATFISMPHVGSFALLLVLSSGFGFGEAVVSSSSAALVADSSEFKRLG
                     AGMGMQGTVMDIGHASGPLLAGLLIAHVSYQGAFAVIAGLQILAAIAFWATMRTLSR"
     gene            complement(1999..2805)
                     /locus_tag="PKK81_06465"
     CDS             complement(1999..2805)
                     /locus_tag="PKK81_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32551.1"
                     /translation="MSRFRPVARRAGLIGVVLTAGLAVLASVGRTESDRCSLPAHHAG
                     VVFPLDQVEAAWACRLEPIVTHYTTANKVGPQRTPLPQPVFLYLLDHPVMAAMLVNRL
                     DLGLYKAEQRGLGEFWATDGEGTEGIVHSLFRDPATRIYYVEGSHDGRFLPRVSGKAV
                     VLLRLHPVTDNRGVESIDSTMVAYLRLDNRFLSGMLSLLRPLIGNVVNRQILKAFDAA
                     RRLGGTMREHSEQVLFEATDPPALPDDEVAFLKAALAGLHNPVESLRPTP"
     gene            complement(2802..4133)
                     /locus_tag="PKK81_06470"
     CDS             complement(2802..4133)
                     /locus_tag="PKK81_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32552.1"
                     /translation="MRHSFRPFDRFHQRRLFLLPVLFSCLLWASLSIPATHAAEEGAS
                     TDLASQVWQYLTTQDVEQQRTVLASIVQRPDATVQAVQELLRKGPPHGLQPVGLLPDE
                     QIVVRERPYQLSLSVPQSYEPTRDYALIVCLHGAGFTGEAYLDRWKSRLGEGYILACP
                     TYPAGAWFTRRAEDLVLATVQAVQQRYRIDPNRIFLSGMSNGGIGAWLIGMHHAPLFA
                     GLAPMASGIDDVLFPFLENLRTTPTYIIHGSQDQVMPVELSRKLAGELKNLGYPYIYR
                     EHDRTHAMAGGHFFPREELPDLVKWFDDQRRTPVPKALTVVRDASHLLPFGWVRIDAT
                     DQIASFSEDLVDKRDASIRQRIYARLTAQVTGPNRIEVKTDRVRQYTLFLNEELVDLS
                     KPVVITTDGQVSFEGVVTPQVDTLLRQARLRQDPGQLYPAHLTLSLPQRPS"
     gene            complement(4141..5268)
                     /gene="ribD"
                     /locus_tag="PKK81_06475"
     CDS             complement(4141..5268)
                     /gene="ribD"
                     /locus_tag="PKK81_06475"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNV32553.1"
                     /translation="MRLSSRDREYMTLALRLAAKGRGSTSPNPMVGALVVTRGRIVGQ
                     GSHRKAGGPHAEVIALSQAGSRAKGGTLYVTLEPCSHLKKRTPPCVPLVIASGVRRVV
                     VAMVDANPQVKGNGVAQLTRAGIRVDVGCCEAEARQLNEAYLHWVQTGRPFTILKAGM
                     TLDGQIATAKGESQWITDEAARQQAHRLRADVDAILVGIGTVLRDNPQLTARLTDGPA
                     RLAPRQPLRIVVDSRLRIPLKAKVLQDQQTAHTLIATTAAASGRKIALLRKRGIDVLV
                     LPKAGGHVNLPVLWARLGQLGVTTVLVEGGSDLNAAVLQAGLPQRLMWYVAPLLLGGQ
                     DAKGIFGGQSPHRLRNAVPLKNLRIEPVGRDMLIQADFSTR"
     gene            complement(5265..8114)
                     /gene="ppdK"
                     /locus_tag="PKK81_06480"
     CDS             complement(5265..8114)
                     /gene="ppdK"
                     /locus_tag="PKK81_06480"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNV32554.1"
                     /translation="MAKKYVYYFGDGKAEGTGNMKELLGGKGAGLAEMTNLKVSVPPG
                     FTISTEACVEYYKRGKAYPPGMIDEALHALKRIERSMKAGFGDPDNPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVHGLALKTKNERFAQDSYRRFIGMFGSIVMGVNREHFEDI
                     LKHKKRDLGVTQDTHLDAKALKELVVSFKELVKEETKRDFPDDPLEQLRMAINAVFSS
                     WYGARAVTYRRLYNIPETWGTAVNVVAMVFGNMGETSGTGVAFTRDPATGQRQFFGEC
                     LTNAQGEDVVAGIRTPLPVKELEKFMPEAYKDLEATYKKLERHYRDMLDLEFTIQEGK
                     LYMLQTRVGKRTGVAAVRIAVDMVKEGLITKKEALQRIGPDQLAQYLYPIFDAQEESR
                     CTPLGKGLPAGPGAAAGKLALTADRAVEMKAAGNRVVLVRQETSPDDIHGMNAALGFL
                     TARGGMTSHAAVVARQMGKVCVAGCEAIEVLDSQSVRIGTQVFREGEYLSVNGSTGNV
                     YGGDIPVVESEVIQVLQGKMEASASEKYQLFESVLKWADGVRKLKVRANADVPDQARI
                     ARSFGAEGIGLCRTEHMFFAEDRIQIMQKMILARKREEREMYLDQLLPLQKQDFIGLY
                     REMKGFPVTIRLLDPPLHEFLPKREDLMVEIAQLELTSGAPTVLEEKKRLLARVEELH
                     EFNPMLGLRGCRLGITMPEITKMQARAIIEAACELAKEGTKIVPEIMIPLVGMVSEMK
                     AQKDLVREVATDTMKRYGVKLSYLVGTMIELPRAAVTADRIAEEAEFFSFGTNDLTQT
                     TFGFSRDDAAKFIDFYKTANILDSDPFAVLDREGVGSLMRTAIVGGRKTRPTIKLGIC
                     GEHGGDPSSVEFCHQLGLDYVSCSPYRVGIARLAAAQAALAEAETEKAKPKKVQPSTK
                     RVAKSKPAKKSATHAPVKKRPAAKRVVRRPKRTKR"
     gene            complement(8140..8658)
                     /locus_tag="PKK81_06485"
     CDS             complement(8140..8658)
                     /locus_tag="PKK81_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32555.1"
                     /translation="MDSTSESLPESPEASAAAQLRATKVRRFASGISVVMMVVCNAVF
                     LFGIWVTGVNFERLVRTPDIYDSRQDICLRLAYQHVPGSENPVQFCSEWLNLADPTGK
                     THTFQKDAQLKQGADGKFYFDYGPFVDYRLFAVGAFVVGIIVCGIRVTRHVVNRYRLR
                     LEAAARHSVPTH"
     gene            complement(8673..10865)
                     /gene="glyS"
                     /locus_tag="PKK81_06490"
     CDS             complement(8673..10865)
                     /gene="glyS"
                     /locus_tag="PKK81_06490"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNV32556.1"
                     /translation="MPTKKTMTKTASRSKVAKAPKAPATTELLLEIGTEELPYQFVAP
                     AMRALQQSAETLLKDLRLTYGTVRTMGTPRRLVLLIEGLARQQASAVKEAMGPSKAVA
                     FDQNGQPTRAAVGFAAGHGIPVEDLQVRQTPKGEYLFAVKQEKGQAVAAVLTQALPQL
                     LGKLSFPKAMHWNQTGVRFARPVRWLVALCGGKVLPIQFATIKAGNLSHGHRVLGAKV
                     SSPKGFAVKSIAHYVKETERHGVIVDQERRRAMILDQLASLAKSARGHLHQDDDLLEQ
                     AVYMVESPLTILGSFKPHYLSLPKEILMTSMKEHQGYFSLVDQKGALLPNFLAVTNMK
                     LSNMQLIREGNERVLAARLADAKFFFDEDRKTSLVDRVAKQLAVTFHQKLGSLHQKTQ
                     RVVAMAAHVAGQLGDDRLVQDCRRAAELSKADLLTGIVGEFPTLQGLMGGEYAKHDGE
                     SPVVSAAIREQYMPRAMEGELPESLAGKVLSLADRLDSIAGFFLVGMVPSGSEDPFAL
                     RRHATAIVRIVIESKLRLNLAQAVRTAQEVLSAQDVIAAPQTGKGGTPDIIGFLFERV
                     RFYGKSAHQVRDDVMEAVLKSADRQTLDLTDLFDKMKALQQITVRAEFDPLIVGFKRA
                     HRLTEKEQWDRKPVESGLFREGAESALHQTVQSSQQDYAAAMRGGQYGQALDVLVRMK
                     GPIDDFFNAVMVNADDPAVRGNRLSLLKSVDDLFMSFADFSQIMVQGT"
     gene            complement(10886..11806)
                     /locus_tag="PKK81_06495"
     CDS             complement(10886..11806)
                     /locus_tag="PKK81_06495"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNV32557.1"
                     /translation="MNFQDLILTLHRFWADRGCVVHQPYDLEMGAGTFHPATFLRSLG
                     PEPWRAAYPQACRRPTDGRYGENPNRMQHYYQYQVVLKPAPDNIQSLYLESLKQLGID
                     PKKHDIRFVQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIGGIPLNPITGEITYG
                     TERIAMYLQQVDNVYDLAWTDGVSYGDVHHRSEVEFSRYNFEEGEVPMLMATFQAFEG
                     ECQRLLDKKLTLPAYDYCIKTSHMFNLLDARGAISVTERTSYIARVRALARRCAESYL
                     ADREAMGHPLIKQSAGTGKRAGAHSPVPTK"
     gene            12049..12474
                     /locus_tag="PKK81_06500"
     CDS             12049..12474
                     /locus_tag="PKK81_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNV32558.1"
                     /translation="MKFSKKSEYGLRALLELCETYGGRVLQRHEIAERQNIPVEFLEQ
                     ILLALKRAGLLASRRGVRGGYSLIKSPEEITLGQVIRILDGPLAPISCVSKTAYQKCA
                     DCPYAAKPSCPLQQAMGEVRDAIANILDHYTLSRFAHSK"
     gene            12497..13552
                     /locus_tag="PKK81_06505"
     CDS             12497..13552
                     /locus_tag="PKK81_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV32559.1"
                     /translation="MTRQTRTIFGLALGAWLTMLLPTTAPVQAAETRDLILAAYSVPK
                     EAYERHIIPAFQRHWKQKTGQDVRVRSSYGASGAQARAIIGGFDADVAVLSLEGDVDQ
                     IVKAGLITHDWRKAPHGGMISASVVALGVRKGNPKGVKGWDDAARPGVEVLYPNPKTS
                     GGAMWDVIAIYGAGLKLAEQRAGGKPLAPEAAASQAVDLLTRVQRNVKVMDKSGRESV
                     TTFERGVGDVIVTYENELLPRVKSGRPYEIIVPAETVWIENPAAVVDTYADRHKARDL
                     AEAFVAFLHGPEAQAAFVELGFRPLDKNAGTQATTLPLPARLFTIADLGGWDQVSTKL
                     FGAQGLWTKIVEDVAKK"
     gene            13655..>14256
                     /locus_tag="PKK81_06510"
     CDS             13655..>14256
                     /locus_tag="PKK81_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV32560.1"
                     /translation="MTAHLLLSLALRSAAVGYVLVLIFLPLAALAHQSFAAGWDRFIQ
                     DLSAPQAAAALWLTVETAAIATAINALFGTLSALVLVRYEFPGRWLLNALVDLPFAIP
                     TLVAGLMIAAIYGPTSLLGTWLQQGGMAVLYNQPGIILAMVFVTMPFTIRSLQPVLMG
                     LERDQEEAAFTLGASPWITFWKVTVPSILPGLLTGVFLTFV"
BASE COUNT         2870 a         4473 c         4142 g         2771 t
ORIGIN      
        1 ttcagataat ccgtatcgat aaccggcgtt ggttttgggg ggaggagacc gcgctcctgg
       61 agcaggcgct ctacgtattt gccgatcagg tctgattcca ggttaagctt gtcgcccacc
      121 tgtttcagtc cgagtgtggt gacttttgca gtgtggggaa tgatcgagac gacaaacgag
      181 cgttcggtca cgtcgttgat ggtgagactg atgccatcga ccgtgatcga tcccttctga
      241 acacagaggc gcagaatctc cttcggcgcc tcaatctcca gaatcagcgc gttcccgtcc
      301 tgatgccggc tccggatggc accgatgcca tccacatgtc ctgacaccat gtgcccgccg
      361 atgcgctcgt tcaacttcat ggcacgctcc agattcacgg gcgaaccgga agtgagaccg
      421 ccgagcgtcg tcaccgcgag ggtttcaggc gagacatcga ccgagaagtc atggtcggtt
      481 ctggcgaccg ccgtcaggca ggtcccattg acgctcacgc tggcgccgat ggtgagatcg
      541 ctcatgatcg tcgaggcgat gatggtcaga cgcgtgccgg ccagcccctt attcagtacg
      601 gagaccgcgc ccatctcttc cacgatgccg ctgaacatgg cttacgctcc cttgcgagtc
      661 ttcaagagat agagacaaaa acccagtagt ccgatcccgc ccacgacggc aatgccgcgc
      721 tgcagcgttt cgataaaatc cgtgtccatc cttctccgct tccttttgca aaatgtgacc
      781 gtccccaggc gaaaagcggc ggcattatag cactaccgcg agagagttct catcgtggcc
      841 cagaaggcga tggccgccag gatctggaga ccggcaatca cggcaaaagc cccctggtaa
      901 ctcacatggg cgatcagcag accggccagc aggggcccgc tggcatggcc gatgtccatc
      961 accgtcccct gcatacccat ccccgccccc aatcgcttga attcggaact gtctgcgacc
     1021 agcgcggcgg acgacgacga caccacagcc tctccgaatc caaacccgga cgaaagcacc
     1081 agcaacagag caaatgagcc gacatggggc atgctgatga aggtcgcggc gcaaatcagg
     1141 aggccggcca gaatcagcgg ctgccgccct acgcggtccg agacccgccc catcaccggc
     1201 ttggaaagaa atgacgtcac cgcctgcaca ctaaacagca gccccacctg tcccgcgttg
     1261 aggccgactg aaagaccata cagcggcagg aatgccatca gggcgccgtt ggccaccatc
     1321 ttggcggcat ctgttgaact ggtgatcaac accttccggt tgcgcgccac ggcgagaaac
     1381 cctttcccca tctcagccat gaccggtgcc aaaccccgct cgcgcacgcg cggcggcggt
     1441 tcatccagat ggagactgaa aaagatcagg atcgcgatac agccgaacac tccggccgtc
     1501 acgaaggccg tggaaaatcc tgccgtgtag gccaaccagc cacccaggaa cgggccgagc
     1561 agagaaccgg attgggtgca ggccgtatag gttcccagcg ctgcgccgcg ccgttcctta
     1621 tacagatcgg ccacggtggc cagggcactc ggcgcaaaga tggccgtggc caggccatgc
     1681 acaaaccgca gcgccgtcag cgcgttgaga tccgagatga aggggtagat gaacggcggc
     1741 agtccgaacg ccaccacgcc gatacgcagc agcatcttcc gtccatagat atcggagagg
     1801 gcacccgagg gcaatttcag caacacaccg gtgatggtcg agaccgacac gatcaacccg
     1861 atccgctcag ggcctgcacc cagcgattct gcaaagaggg ataagaccgg catgcggacg
     1921 aggttgtagc tgatgaagca gaacacgcca agggtgcaga gataaaaaaa acttcgggaa
     1981 gtcgtcatgg atatctcatc agggagttgg gcgaagcgat tcgacaggat tgtgcagacc
     2041 cgcaagagcg gctttcaaga aggccacctc gtcatccggc agagccggtg gatccgtcgc
     2101 ttcaaacagg acctgctcgg aatgttcgcg catcgtgcca cccaaccggc gggcggcatc
     2161 gaaggctttc aggatctgac gattcaccac attcccgatc agcggacgga gcagcgacag
     2221 catccccgac aggaagcgat tgtcgagtcg caggtaggcg accatcgtac tgtcgatcga
     2281 ttcgactccc cggttgtcgg tcacaggatg cagcctcaac aggaccaccg ccttgccgct
     2341 cacccgcggc agaaaccgtc cgtcatggct tccctccacg taatagatac gcgtcgcagg
     2401 atctcgaaac agggaatgga cgatcccttc cgtgccttcc ccgtcggtgg cccagaattc
     2461 ccccagcccg cgctgctcgg ccttatacag cccgagatcg aggcgattga ccagcatggc
     2521 ggccatcacc ggatgatcca ggagatagag gaagaccggt tgcggcaaag gcgtgcgttg
     2581 tggtccgact ttattggcgg tggtgtaatg cgtgacgatc ggctcaagtc ggcaggccca
     2641 ggcggcctcg acttgatcca gcggaaagac gacaccggca tggtgtgcgg gaagggagca
     2701 ccggtccgat tcagttcgcc cgacagacgc gaggaccgcc agcccggctg tgagcacgac
     2761 cccgatcagg ccggctcgtc gcgcgaccgg cctgaacctg ctcatgaagg ccgttgcggc
     2821 agtgagagcg tgagatgagc gggatacagt tgcccggggt cctgcctgag ccgcgcttgc
     2881 cgcagcagcg tatcgacctg tggcgtgacg acgccctcga aggaaacctg cccatcggtg
     2941 gtgatgacga ccggctttga cagatcgaca agttcttcgt tcaggaacaa tgtgtattgc
     3001 cggacacgat cggtcttgac ctcaatccga ttcggcccgg tcacctgagc ggtcagcctg
     3061 gcgtagatgc gctgccggat actcgcatcc cgcttatcga ccaaatcttc tgaaaaggat
     3121 gcgatctggt ccgtcgcatc gatccgcacc cagccgaacg gcagcaaatg gctcgcatcc
     3181 cgcacgactg tcagcgcctt cggaacagga gtccgccgct ggtcgtcgaa ccacttcacc
     3241 agatcgggaa gttcttcacg cggaaaaaag tggcccccgg ccatcgcatg ggtgcgatca
     3301 tgctcgcgat agatgtaggg atacccgagg ttcttgagtt ctcccgccag cttccggctc
     3361 aattccaccg gcatcacctg atcctgcgat ccatgaatga tgtaagtcgg cgtcgtgcga
     3421 aggttctcca gaaacggaaa caagacgtca tcgatcccgc tggccatcgg cgcaagtccg
     3481 gcaaacaggg gcgcatgatg catgccgatc agccaggcac cgataccgcc gttggacatg
     3541 ccgctcagga agatgcggtt gggatcgatc cgataccgct gctgcacggc ctgcacggtc
     3601 gccagcacca gatcttccgc gcgtctggtg aaccaggctc cggctggata ggtcggacag
     3661 gccaggatgt agccctcgcc caagcgtgat ttccatcgat ctaagtaggc ctcgccggtg
     3721 aatccggcgc catggagaca gacgatcagg gcataatctc gggtcggttc ataactctga
     3781 gggacggaca agctcagctg atacggtcgt tcgcggacga cgatctgctc atccggcagg
     3841 agtcccacgg gctgcaaccc gtgaggcgga cctttgcgca acagttcctg tacggcctgc
     3901 accgtcgcgt ccggtcgctg cacaatcgac gcgagcacgg tgcgctgctg ttcgacatcc
     3961 tgggtcgtga gatattgcca gacctgcgag gccaggtcgg tcgaggcccc ctcctccgcg
     4021 gcgtgggtgg ccggaatcga aagactcgcc catagcaggc acgagaagag aaccgggagc
     4081 aggaaaagtc tgcgttggtg aaatcggtcg aagggccgga aagaatgtcg cacaacgctc
     4141 ctaacgagtc gagaagtcag cctgtatcaa catatcacgc cccaccggct cgatgcgcag
     4201 attcttcagg ggtacggcat tccggagtcg gtgcggcgac tgcccgccga agattccttt
     4261 cgcatcctgc ccgccgagca gcaacggggc gacataccac atcaatcgct ggggcaatcc
     4321 ggcttgcagc acggccgcgt tcaggtcgct gccgccttcg accaagaccg ttgtgacgcc
     4381 aagctgaccg aggcgcgccc acaacaccgg caagttcaca tggccaccag ccttcggtaa
     4441 gaccaacaca tcgatccccc tcttcctcag aagcgcaatc ttacgccctg aagctgccgc
     4501 tgtcgtagcg atcaacgtgt gagccgtctg ctggtcctgc agcaccttgg ccttgagggg
     4561 aatacgcaat cgactatcga ccacaatgcg caacggctgc cgcggagcca accgcgccgg
     4621 gccgtcggtc agccgggcgg tcaactgtgg attgtcgcgt aacaccgtgc cgatgccgac
     4681 caggatcgcg tctacatcag cccgcaagcg atgggcctgc tgtctggcgg cctcatccgt
     4741 aatccattgc gactcgccct tcgcagtggc gatctgcccg tccagcgtca tcccggcttt
     4801 caggatcgta aacggacggc cggtctgcac ccaatgaaga taagcttcat tgagctgcct
     4861 cgcctcggcc tcgcagcaac cgacatcgac acgaattccc gcccgcgtca gctgcgcaac
     4921 cccattgccc ttcacctgcg ggttggcatc gaccatggcc accacgacgc ggcggactcc
     4981 cgaggcaatg accagcggaa cacagggtgg agtgcgtttt ttgagatgac tgcagggttc
     5041 gagggtgacg tagagggtgc cgcctttagc ccgtgagccg gcctggctga gggcgatgac
     5101 ttcggcatgt ggaccgccgg ctttgcgatg ggaaccctga ccgacgatcc ggccgcgggt
     5161 caccaccaat gcgccgacca tgggattggg gctggtggat ccccggccct tcgccgcgag
     5221 gcgaagcgcc agggtcatgt attcacgatc gcgggaagac aacctcaccg tttcgtacgc
     5281 ttggggcgac ggaccacacg cttggcggcg ggccgcttct tcaccggcgc gtgcgttgcg
     5341 gacttcttgg ccggcttgga tttcgccacc cgcttggtgc tcggttgaac tttcttcggc
     5401 ttggcttttt ccgtttcagc ctccgccagc gccgcctgtg ccgcagccaa tcgcgcaatg
     5461 ccgacccggt agggtgaaca actcacatag tccaggccca gttgatgaca gaattccaca
     5521 gaactgggat cgccgccatg ctcgccgcag atgcccagct tgatcgtggg acgggtcttg
     5581 cgcccgccca caatggccgt gcgcatgagc gatccgacgc cttcccggtc cagcaccgca
     5641 aacggatcgc tgtcgagaat attggccgtc ttatagaaat cgatgaattt cgcggcgtcg
     5701 tcgcgggaga acccgaacgt cgtctgggtc agatcgttcg tcccgaagga gaagaactcg
     5761 gcctcctccg cgatacgatc ggcggtcacg gcggcgcgcg gcaattcgat catggtgccg
     5821 accaggtacg acaatttcac gccgtaccgc ttcatcgtgt ccgtggccac ttcgcggacc
     5881 agatctttct gcgccttcat ttccgagacc atgccgacca agggaatcat gatctcaggc
     5941 acgatcttcg tgccttcctt cgccagctca caggccgctt cgatgatcgc gcgcgcctgc
     6001 atcttggtaa tttccggcat cgtaatcccg agacggcagc cgcgcagacc cagcatgggg
     6061 ttaaactcat gcagctcttc cacgcgagcc agcaaccgct tcttttcctc caacaccgtc
     6121 ggcgcgccgc tggtgagttc gagctgggca atttcgacca tgagatcttc gcgcttcggc
     6181 aggaactcgt gcaggggcgg atcgagcaga cggatcgtga ccgggaaccc cttcatctcg
     6241 cgataaagac cgatgaaatc ctgtttctgc aacggcagca gctgatccag atacatttcc
     6301 cgctcttcgc gtttccgcgc cagaatcatc ttctgcatga tctggatacg gtcttcggcg
     6361 aagaacatat gttccgtgcg gcacaggcca ataccttcgg cgccgaaact gcgcgcaata
     6421 cgggcttgat ccggcacatc cgcattcgcc cggaccttca gcttgcgcac tccgtcggcc
     6481 cacttcagca ccgactcgaa caattggtac ttctcggaag ccgaggcttc catcttgccc
     6541 tgcagcactt gaatcacttc tgattccacg acgggaatgt cgccgccata cacgttgccg
     6601 gtcgaaccgt tgaccgacag gtattcgcct tcgcgaaaca cctgggtccc gatccgcacc
     6661 gactgactat ccagcacctc gatcgcttca cagccggcca cgcagacttt ccccatctgc
     6721 cgggcgacca cggccgcgtg ggacgtcatg ccgccacgcg cagtcagaaa gcccaacgcg
     6781 gcgttcatgc cgtgaatatc gtccgggctg gtttcctgcc ggacaaggac cacgcgattg
     6841 cccgcggctt tcatctccac cgcacggtcc gccgtcaatg cgagttttcc ggcggcggcg
     6901 ccgggaccgg ccggcaatcc cttgccgagc ggcgtgcagc gcgactcttc ctgcgcgtcg
     6961 aaaatgggat agagatactg cgccagttga tcgggcccga tgcgctggag cgcctccttt
     7021 ttggtaatga ggccttcctt caccatatcc accgcaatcc ggacggccgc cacacccgtg
     7081 cgcttcccga cgcgggtttg caacatgtag agcttccctt cctggatcgt gaactccagg
     7141 tcgagcatgt cgcggtaatg gcgttcgagt tttttgtagg tcgcttcgag atccttgtaa
     7201 gcctccggca tgaacttttc gagttccttg acgggcagcg gggtccggat accggcaacg
     7261 acgtcttcac cctgcgcgtt cgtcagacac tcgccgaaga actgacgctg accggtggcg
     7321 ggatcgcgcg tgaaggccac gccggtgccg ctggtttcac ccatgttgcc gaacaccatg
     7381 gcgaccacgt tgacggcagt gccccaggtt tcgggaatgt tgtacagacg gcgatacgtc
     7441 acggcgcgcg caccgtacca ggaggaaaac acggcgttga tcgccatccg cagttgctcc
     7501 aggggatcat ccgggaaatc ccgcttggtc tcttccttca ccaactcctt gaagctgacg
     7561 acgagttcct tgagcgcctt ggcatcgagg tgcgtgtcct gcgtcacccc gagatcccgc
     7621 ttcttatgct tgagaatgtc ttcgaaatgt tcgcgattga cgcccatcac gatgctgccg
     7681 aacataccga tgaagcggcg gtagctatcc tgcgcaaacc gctcgttctt cgtcttgagg
     7741 gccaggccgt ggacggtctt ggtcgtcaag ccgacgttca acacggtatc catcatgccg
     7801 ggcatggacg cgcgggcgcc ggagcgcacg gacaccaaca agggattgtc cggatcgccg
     7861 aaaccggcct tcatcgaccg ttcgatccgc ttcagggcgt gcagcgcttc gtctatcatg
     7921 ccgggaggat aggccttgcc gcgtttgtag tattcgacgc aggcttcggt ggagatcgta
     7981 aagccggggg gcacggacac tttgaggttc gtcatctcgg ccagtccggc gcctttgccg
     8041 ccgagcagtt ccttcatgtt gccggtgcct tcggctttcc catctccgaa ataatagacg
     8101 tatttcttgg ccacgcgacg ctctccttgt gctatgaggt caatgagtcg gaaccgaatg
     8161 gcgggcggcg gcttccaatc gcaggcggta gcggttcacg acgtgcctgg tcactcgaat
     8221 gccgcacacg atgatcccca ccacgaacgc gccgaccgca aacagtcggt agtccacgaa
     8281 cggtccataa tcgaaataga actttccgtc tgcgccctgt ttgagttgtg cgtctttttg
     8341 aaacgtatgg gtcttcccgg tcggatcggc gaggttgagc cactccgagc agaactgcac
     8401 ggggttctcc gaaccgggga catgttggta ggccagtcgc aagcaaatgt cttgtctgga
     8461 gtcatagata tccggtgtcc gcaccagccg ttcgaagttg acgccggtca cccagatgcc
     8521 gaagaggaac accgcgttgc acaccaccat catgacgacg ctgatgccgg acgcaaaccg
     8581 ccggaccttg gtcgccctca gctgggccgc ggcactcgcc tcgggtgact caggaaggct
     8641 ctctgacgta gagtccaaac gttcaccatc cgctacgtcc cttgtaccat aatctgggag
     8701 aaatcagcga atgacatgaa caaatcgtcg acgctcttca acagggatag ccggtttccg
     8761 cgcaccgccg gatcgtcggc gttcaccatc accgcattaa aaaagtcgtc gatcggaccc
     8821 ttcattcgaa ccagcacatc gagcgcctgt ccatactgcc cgccgcgcat cgccgccgca
     8881 tagtcctgct gactgctctg cacggtctga tgcagggccg actccgcacc ttcccggaag
     8941 agaccggact ccaccggctt gcggtcccac tgttccttct cggtcagccg gtgggcgcgt
     9001 ttgaatccga cgatcaacgg atcgaactcc gcccgcacgg tgatctgctg caatgccttc
     9061 atcttgtcga agaggtccgt caggtcgagg gtctggcgat cggccgactt caacacggct
     9121 tccatcacat cgtcccgtac ctgatgcgcg ctctttccgt aaaaacggac acgttcgaag
     9181 agaaagccaa tgatgtccgg tgtcccgccc ttgcctgtct gcggcgcggc gatcacatcc
     9241 tgggcgctca gcacctcctg tgccgttcgc acagcttggg cgagattgag tcgcagcttg
     9301 ctctcgatca cgatgcgcac aatggccgtg gcatggcgcc tcaaggcaaa gggatcttca
     9361 gagccgctcg ggaccatacc gacgaggaaa aatccagcga tgctgtccag gcggtccgcc
     9421 aacgacagca cttttcccgc gagcgactca ggcaactccc cttccatggc gcgaggcatg
     9481 tactgctcac ggattgccgc gctcaccacc ggagattcgc cgtcatgttt ggcatattcc
     9541 ccgcccatca ggccctgcag ggttggaaac tccccgacga taccggtcaa gagatcggcc
     9601 ttactgagtt ccgccgcgcg ccggcaatcc tgaaccagtc ggtcgtcacc gagttgcccg
     9661 gccacatggg ccgccatcgc gacgacacgt tgcgttttct gatgcaggct gccgagtttc
     9721 tggtggaacg tcaccgccag ctgtttcgct acgcgatcga ccaacgaggt cttgcgatct
     9781 tcatcgaaga agaacttggc atcggccaac cgcgccgcca gaaccctctc gttcccttcg
     9841 cgaatcagct gcatgttcga caacttcatg ttcgtgacgg cgaggaaatt cggcagcagc
     9901 gcgcccttct gatcgaccag ggagaaatac ccttgatgct ccttcatgga ggtcatgagg
     9961 atttctttcg ggagcgagag atagtgcggc ttaaaggaac ccagaatcgt cagcggagac
    10021 tccaccatat agacggcctg ctccagcaaa tcgtcgtcct ggtgcagatg gccgcgggcc
    10081 gatttggcga gcgaggccag ctggtcgagg atcatcgcgc gccgccgctc ctgatccaca
    10141 atcaccccat gccgttcggt ctccttcaca tagtgcgcaa tcgacttcac cgcaaagccc
    10201 ttcgggctgg acaccttggc gcccagcacc cgatgtccgt gactgagatt gccggccttg
    10261 atcgtggcga actggatcgg gagtaccttc ccgccgcaca aggccaccag ccagcgcacc
    10321 ggcctggcaa agcgtacgcc ggtctggttc caatgcatgg ctttgggaaa cgacagtttg
    10381 ccaaggagct gcggcaaggc ctgggtcaga accgccgcca cggcctgccc cttctcctgc
    10441 ttcaccgcaa agagatactc gcccttgggc gtctgccgca cttgtaagtc ttcaaccgga
    10501 ataccatggc ccgcggcaaa accgacggcc gctctcgtag gctgtccatt ttggtcgaag
    10561 gccaccgcct tagaaggccc catggcctct ttcacggcag acgcttgttg ccgcgcaagt
    10621 ccttcgatca acagcaccag ccgccgcgga gtgcccatcg tgcggaccgt gccgtacgtg
    10681 agacgcaggt ctttcagcag agtctcggcc gattgctgca gagcgcgcat ggccggggca
    10741 acgaactgat agggcaactc ttccgttccg atttccaaca ggagttccgt ggtcgccgga
    10801 gccttggggg ctttggccac cttggagcgt gaggcagttt tggtcatggt ctttttcgtc
    10861 ggcatcgtca ggtactgcct gcggattatt tggttggaac gggtgaatga gcgccggcgc
    10921 gcttgccggt cccagccgat tgcttgatca agggatgtcc catcgcttcg cgatcagcca
    10981 ggtaggactc ggcgcaacga cgggccagcg cccgcacacg ggcgatatag gaggtccgtt
    11041 cggtgacgct gatcgccccg cgggcatcga gcaaattgaa catgtgcgag gtcttgatgc
    11101 agtagtcgta ggccggcagc gtcagcttct tgtccagcaa ccgctggcac tcgccctcga
    11161 acgcctggaa ggtggccatc agcatgggca cctctccctc ctcgaagttg tagcgggaaa
    11221 actccacttc gctgcggtga tgcacatcgc cgtagctcac accgtcggtc caggccaggt
    11281 catacacgtt atcgacctgc tgcaggtaca tcgcaatacg ctcggtgcca taggtaattt
    11341 cgccggtgat gggattcagg ggaatgccgc cgatttcctg aaagtaggtg aactgggtaa
    11401 tctccatgcc gtcgagccgg acttcccagc ccaaccccca ggcgccgagc gtgggggact
    11461 cccaatcgtc ctgcacaaac cggatgtcgt gtttcttcgg gtcgatgccc agctgtttca
    11521 ggctctccag gtagaggctt tggatattgt cgggagccgg tttcagcacc acctgatact
    11581 ggtagtagtg ctgcatacgg ttgggatttt cgccgtatcg accgtcggtc ggccggcgac
    11641 aggcttgcgg ataggcggct cgccagggtt ccggaccgag ggaacggaga aacgtggcgg
    11701 gatggaatgt tcccgcgccc atttccaggt catagggttg gtggacgaca cacccgcggt
    11761 cggcccaaaa acgatgcagg gtcaaaatga ggtcttggaa attcaccggg tatccagatc
    11821 tagcagcagt ctgctggtgg tggctgacaa aaaacaggct gaagctagca agaacggtta
    11881 cagggtgtca agaaacagaa cctccgattt caatgagttg cattcatgat tccggcagca
    11941 cggagcgcga atcacaccag tcgcctggca tctgaaacgc ttgaatcaca gcgcgcgctg
    12001 gtttattctt caaaaacttg agcaactcac tagacaaaat cttccggcat gaaattctcc
    12061 aaaaaaagtg aatatggcct ccgggcactg ctcgaattgt gcgagaccta tggcgggcgt
    12121 gtcctccaac gccatgaaat tgcagaacgg cagaatatcc cggtggaatt cctggaacag
    12181 attctcctcg cgttgaaacg cgccggcctg ctggccagtc gtcgaggagt ccggggcggc
    12241 tattccctta tcaaatcgcc ggaagaaatt acgcttggcc aggtcatccg tattctggat
    12301 ggacccctcg ccccgatcag ctgcgtcagc aaaaccgcct accaaaagtg cgcggactgc
    12361 ccctacgcgg ccaaaccttc gtgcccgctc caacaggcca tgggtgaggt gcgggatgcc
    12421 atcgcgaaca ttctcgacca ttacacattg agtcgattcg cccattcgaa ataggtggaa
    12481 ggagccgcga cccgctatga ctcgtcagac acgaaccatt tttggccttg cactcggcgc
    12541 ctggttgacc atgctccttc cgaccactgc cccggtgcag gccgccgaaa cccgtgatct
    12601 catcctggcc gcctacagcg tgccgaaaga agcctacgaa cgacacatca tccccgcctt
    12661 tcaacgacat tggaaacaaa aaaccgggca ggacgtgcgt gtgcgcagct cttatggagc
    12721 ctcgggcgct caggctaggg ctatcatcgg aggtttcgat gccgatgtcg ccgtgctctc
    12781 gctggaaggc gacgtcgatc agatcgtcaa agccggattg attacccatg attggagaaa
    12841 ggcaccccat gggggcatga tctccgcatc ggtcgtggcc ctcggcgtac gcaagggcaa
    12901 tcctaaaggc gttaagggat gggacgatgc cgcgcgcccc ggcgtggaag tgctctaccc
    12961 gaatcccaag acatccggcg gcgcgatgtg ggatgtgatc gcgatttatg gcgccggctt
    13021 gaaactggcg gagcagcgcg caggtggaaa gcccctcgcc ccagaggcgg cggcttcgca
    13081 ggccgtggac ttgctgaccc gcgtccaacg caacgtcaag gtcatggaca aaagcggccg
    13141 tgaatcggtg acgacgttcg aacgcggcgt cggcgatgtc atcgtgacgt atgaaaacga
    13201 attgttgccg cgcgtgaaga gcgggcgccc gtacgaaatc attgtgccgg ccgaaaccgt
    13261 gtggatcgaa aacccggcag ccgtcgttga tacctatgcc gaccgccaca aggccaggga
    13321 tctcgcagaa gcgttcgtcg cgtttctcca cggaccggag gcacaggctg ccttcgttga
    13381 attgggcttt cgcccgttgg acaaaaatgc cggtacccag gccaccacgc tcccactacc
    13441 ggctcgactc ttcacgattg cagatctcgg cggctgggat caggtgagca ccaagctgtt
    13501 cggcgcgcaa ggtctgtgga cgaagattgt cgaggacgtg gcaaagaaat agcagcctcc
    13561 tttgaaaatg attcgtgatg aatcatcacg accaatgata ggaatggtgc ctgtcgaaca
    13621 tgcgtgaccg taaccagcaa cgtagaacgc tcgtatgacc gctcatctct tgctcagcct
    13681 ggccttacga tctgccgcag tcggctatgt gctcgtgctg attttcttgc ccctcgcggc
    13741 gctggcacat caatcgttcg cggccggatg ggaccggttc atccaggatc tgtcggctcc
    13801 ccaagctgcg gcagcgctgt ggctcaccgt ggaaaccgcc gccattgcga ccgccatcaa
    13861 tgccctgttc ggcaccttgt cggcactggt gctggtgcgc tacgaattcc ctggccggtg
    13921 gctcctcaac gcgctcgtcg atttgccctt tgccattcca accctggttg ccggcctcat
    13981 gatcgccgcg atctatggac cgaccagtct gctgggcacc tggcttcaac agggcgggat
    14041 ggcggtactc tacaaccagc ccggcatcat cctggctatg gtgtttgtga cgatgccgtt
    14101 taccatccgc tctctgcagc cggtgttgat gggacttgag cgcgatcagg aagaagcagc
    14161 cttcacgttg ggagccagcc cctggatcac cttctggaag gtcacggtcc cgtcgatcct
    14221 gcccggccta ctgaccggcg tcttcctcac cttcgt
//
LOCUS       DAOQRM010000058        14126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3367_length_14126_cov_16.204108, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000058 DAOQRM010000000
VERSION     DAOQRM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14126
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3367_length_14126_cov_16.204108"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PKK81_06515"
     CDS             <1..726
                     /locus_tag="PKK81_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNV32561.1"
                     /translation="RGPFVIKQSRVKGRIVNRLKSGFLLITGPVVLAHTDFAGFVDLS
                     RTVFLGLVDGSNATFHQESYFVQDRFTQGAMFSDTHFGPHARFHRSVFAGPAIFRGAT
                     FQGLTEFLEVVFEQDANFSRAAFHLGTGFSGAHCRAKCDFSSSQFDREAFFLFAIFDR
                     PATFASARFGSQADFSDAAFKQADDLAQATFARTPQLTRTARVSTTVPGPTASTSAPF
                     SQAVTIILFVMALGLLVYIIRAK"
     gene            825..1604
                     /locus_tag="PKK81_06520"
     CDS             825..1604
                     /locus_tag="PKK81_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNV32562.1"
                     /translation="MDQPELPYQVKIENFEGPLDLLLHLIKKNEINIYDIPIALIAQQ
                     YLDYLSVMKELNLAVAGEFLVMAATLLHIKSRMLLPVDEVAVDEEDGPDPREELVRRL
                     LEYKQFKEAASQLDYKERLWRDVFSREPGPPVEVKKDESLLDEISLFDLVDALQGVLS
                     RHPGKRLVEIIPDNLTVRTRMSAIIEALELHESMAFTALFEESSHRLVVIVTFLALLE
                     LIRIRVVRVFQSETFGAILVSRAFSAVAEGELVEESEWKNL"
     gene            1589..2506
                     /gene="scpB"
                     /locus_tag="PKK81_06525"
     CDS             1589..2506
                     /gene="scpB"
                     /locus_tag="PKK81_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNV32563.1"
                     /translation="MEEPMSPGVEEAIEESVEVRDPVDEPTASLDAVEVVPESGQGVV
                     HDMAAPAADSALSDMRALKGILEALLFVTAEPIPVTRFLALLGAVTKQDVDQALASLA
                     QDYEQEGRGLQLAEVAGGYRIVTKAEFAPWLKRLEKVKSPSKLSRSALESLAIIAYKQ
                     PIVRAEVEQIRGVETSGVIRTLLERKLVRIVGRKEEPGRPIMYGTTKFFLEHFGLRDL
                     SQLPPLREFKELGESEQAMLPIDEVVAVDGEPVTQSTEAVVEGLSEGMDEPVALLEPA
                     DEAGQTSESLVLENEAGGDLVVADIAEES"
     gene            complement(2675..3241)
                     /locus_tag="PKK81_06530"
     CDS             complement(2675..3241)
                     /locus_tag="PKK81_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32564.1"
                     /translation="MWPTRLVLATLAASLLLTPSVHAVESSPVEMWECPGPEGTILYT
                     NKERPDCQAKVLKALSVVPSLPDLPLAPPNGSTYQPTPKAKDFSYDTPIGALRNLSQI
                     PESGRDWYAGNASGGSVQGEVCSMYMEWIHLNQNSRGGHFFGTDPSYGSNPTARNWYT
                     PSYSFYDNARYVTLSQIFGAGFVPIGCH"
     gene            3560..6529
                     /locus_tag="PKK81_06535"
     CDS             3560..6529
                     /locus_tag="PKK81_06535"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNV32565.1"
                     /translation="MIKAWLLDEMFRFDRRYLTAEGSQSEWGAGDSVAEEEDLPPAPT
                     GVAAQAGNGRVMITWDAVPEAMYYNLYFMTTKGVQIKFSELTRPIASAEDFKTVIGVT
                     KEKGTCIEGAQSPFMHDDLANGTCYHYVVTVVTQKGESPESQEVMAIPAPYLIAKIIG
                     QEGVEDGEFSSPTGIAVDKDGNIYVADTDNHSIQKFDKDGKFLGRWGGEAGSAEGGFY
                     YPRGLTTNSDGQVYVADTGNNRVQRLDTDGNPMKAWGKFGFAWRGADMNKFDAPWGVT
                     TDQDGNIYVTDTNNARIQKFKGDGTPLLKWGRDGSFDGAFFFPRGVAVDFVGNTYVAD
                     EGNNRIQKFDTRGSFLTKWGREGSGPGQFKAPWGVTCDALGNVYVVDQGNHRIQKFDG
                     NGTFLCAWGNRGKTEGQLNFPSGVAVDKEGAVYVVDSGNHRVIKYVPTDEELNRGKAE
                     RERAQAVSEYPIPRNLAIKPGDTETFLSWMDVPGAVSYNLYFHTSANLTQEGGTKIEG
                     VTSPYNHSGLTNDQAYYYALTAVYEDGTESGLSDEVSATPVLIDITAPQTPYAVINHG
                     AFMTNSPEIVVTISATDLDTGVAAYYISENPMTPMAGTPGWVEVPPAIKFGATIPFIL
                     SPGDGQKTVIVWFKDLGNNISTPASATILVNTSGYLCVSKWGKPGRGASLLHGGEFMA
                     PMYGLAIDQQGSIFVVDNGNNRIQKFDRTGNFIILWGNFGAANANFHNPTGIACDAKG
                     DVYVVDTNNHRVQKFDGKLGGYLMKFGSRGNGEGQFNAPWGIAIDRVRGYIYVVDSAN
                     FRVQKFDMSGEFIMSWGSFGNGDGQFYFPRGIAVDQADGTVYVVDMGNHRVQKFDTST
                     NVLPQLLTKWGGSSEAGHASSPLAQEAGQLRSPWGIAVDGQGDVYVTDTGNHRVEKFD
                     REGNFIAQWGGFGGGDGQFNFPYGIVVDSRGSVFAVDSGNTRVQQFMPADEGSERLQE
                     EADAAAELGQVDKTTRV"
     gene            6568..8034
                     /gene="guaB"
                     /locus_tag="PKK81_06540"
     CDS             6568..8034
                     /gene="guaB"
                     /locus_tag="PKK81_06540"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNV32566.1"
                     /translation="MLEKEIRLGLTYDDVVLVPAKSQVLPSEVDTRTRLSRNIQLNIP
                     IVSAAMDTVTEARLAIAMAQEGGIGIVHRVLSPTDQAAEIDKVKKSESGMILDPITIS
                     PDQTIRDAHDLMARYRISGIPVTKSGKLVGILTNRDLRFETRMELKVSQVMKRDKLIT
                     APEGTSLEKAREILHEHRIEKLPVVNKQFDLKGLITIKDIEKRIKYPNACKDAHGRLR
                     VGAALGVGPDTADRVALLVKAGVDVVVVDTAHGHSQAVLDTVKMVRKAHPKLDIIAGN
                     IATAQAAKDLVKAGVDAVKVGVGPGSICTTRMVSGAGMPQLTAIADCARALAGSGVPV
                     IADGGIKYSGDITKALAAGASVVMLGSLLAGTEEAPGETVLFQARTYKVYRGMGSIGA
                     MERGGGDRYGQGGRPTPKLVPEGIEGRVPYKGTLAPHIYQMVGGVKSGMGYCGCKTIP
                     DLQQKATFIRQTVAGLREGHVHDVIITKEAPNYRTDWE"
     gene            8126..9682
                     /gene="guaA"
                     /locus_tag="PKK81_06545"
     CDS             8126..9682
                     /gene="guaA"
                     /locus_tag="PKK81_06545"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNV32567.1"
                     /translation="MELWHDRILVLDFGSQYTQLIARRIREAQVYSQILPCTVPLATV
                     LAYRPKGIVLSGGPSSVYDKKAPSVSKQLFEQGIPILGICYGMQLVTHLQGGEVEKAP
                     HREFGRAELTLDDRSDLFKGIGTRGSTVVWMSHGDRIERMPPGFRSIAHTDNSPIAAM
                     KRHDARQRIYCLQFHPEVAHTTEGATILKNFVYDICGCKPTWTMRSYVDTAVEQIRQQ
                     VGTERVICALSGGVDSSVAAALTHRAVGDQLTCIFVDNGVLRTGERDQVEKTFASQMH
                     LNMRILDRTSQFLTGLKSVTDPERKRKIIGRLFIKNFEVESKKLKGIKYLVQGTLYPD
                     VIESVSFKGPSATIKTHHNVGGLPTRMKLKLVEPLRELFKDEVRALGQELGLPDEIIW
                     RQPFPGPGLAIRVLGAVTKERLAILRGAETIVDQEIRAAGLYREIWQAFAVLLPIRTV
                     GVMGDQRTYEYVIAIRAVTSLDGMTADWAKIPSDVLGKMSSRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
     gene            9778..11040
                     /locus_tag="PKK81_06550"
     CDS             9778..11040
                     /locus_tag="PKK81_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV32568.1"
                     /translation="MEVRLQKLIAGTGLASRRKAEELITSGRVTINGSVVKELGTKVD
                     PARDHVKVDGKHLRAAQPYVYLILNKPKNVMSTLDDPEGRPTVKDLLHGVTVRVFPVG
                     RLDFDSEGLMLLTNHGDLAQALLHPRYHVPKTYLIKVKGVLDDAKIEALERGVKLEDG
                     FTAPAKVNKVSKAESNSWLEVTIHEGRKHQVKRMVEAVGHSVIKLVRVRMGPLLLGDL
                     APREYRFLTDREANRLRDLVEDRVARAERPELDASGKPTRWMPPTEPAPPRPPKPERS
                     GRGPKAQRSERGPRSQRSLASAKPSRSGRGTTRRPMSPGAKFRRPAKGVGFQRTQAGP
                     IPQGADRGPGSRPTGPDQKPQRTQPGAGFQRPRSGAKPQGPGRGMTVRRSGPGQNFQQ
                     KRRGGSSASRPGQRGPAGKSRPSTRRRA"
     gene            11037..12800
                     /gene="aspS"
                     /locus_tag="PKK81_06555"
     CDS             11037..12800
                     /gene="aspS"
                     /locus_tag="PKK81_06555"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNV32569.1"
                     /translation="MKIRTHRCGELTKAQVGQQVVLNGWVQRRRDHGMVLFIDLRDRT
                     GLTQVVFNAERNAAVHQAAHTLRSECVVSVTGQVMARPDESKNPNLPTGEIEVFVDAV
                     EILNEAKTPPFMIEDDIEITESLRLKYRYLDLRRPRMQRLLGLRHGIMQAVRAFMNAE
                     QFLEVETPVLTKSTPEGARDYLVPSRVNPGMFYALPQSPQLFKQVLMVSGVDRYYQIA
                     RCFRDEDLRNDRQPEFTQIDLEMSFVDREQVMTLMEHMIVSVFKDAGGVQLPTPFPRM
                     TYADAMGRYGSDKPDLRFDMPLHDVTAFAAKSEFKVFKEAATKGGLVKALIVSGGASI
                     ARSRIDALGETAKSFGAKGLAWLKVTADGQLESVIAKFLDAKAFAEALPEAKPGDLVL
                     FGADKPAIVHDVLGRIRLLLGEELNLIDKTAWKPLWVTEFPLLDYSPEEKRYIFMHNP
                     FAAPMDEDLPFLDTEPLKARAKAYDMVLNGSEIGGGSIRNHRSDIQLRILDLLGIGKE
                     QAQAKFGFLLEALEYGAPPHGGIAFGLDRLIMLLGGADSIRDVIAFPKTQRAQCPLTE
                     APSGVSAEQLKELRIKLDLVE"
     gene            12819..13955
                     /gene="aroC"
                     /locus_tag="PKK81_06560"
     CDS             12819..13955
                     /gene="aroC"
                     /locus_tag="PKK81_06560"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNV32570.1"
                     /translation="MAGNTLGRVFTVTSFGESHGPAIGCVVDGCPPGLALSAEDIQRD
                     LDRRKPGTSRHVTQRQESDTVEILSGVFEGKTTGTPIALLIRNEDQRSRDYGNLLDTF
                     RPGHADYTYWQKYGIRDHRGGGRSSARETAVRVAAAAIAKKWLQEKHGVVIRGYLSQL
                     GPIEAPFQSWETVDTNPFFSANAEVVGKLESFMDELRKAGDSVGAKITAMAEHVPVGW
                     GAPVYAKLDADLAGAMMSINAVKAVEIGAGFASVAQRGSEHGDELTPEGFVTNHAGGI
                     LGGISTGQDVVVTIAIKPTSSIRIPRRSIDKQGNPVTVETNGRHDPCVGIRATPIAEA
                     MMALVLMDHALLHRAQNADVQTATPKIPGSSTHGAVPASKKPTA"
BASE COUNT         2955 a         3823 c         4458 g         2890 t
ORIGIN      
        1 cgcgggccat tcgtgatcaa gcagtcgagg gtgaaaggac ggatcgtcaa ccggctcaag
       61 agcgggtttc tgttgatcac cggaccagtg gtgttggcgc acacggattt cgccggattc
      121 gtcgacctct cgcgcaccgt gtttctcggc ctggtcgatg gatcgaatgc gaccttccat
      181 caggagagtt attttgttca ggatcgattt acgcagggag ccatgttttc ggatacgcat
      241 ttcggcccgc acgctcgatt tcaccgctcg gtgtttgccg gcccggcgat ttttcgcggg
      301 gcgacctttc aggggttaac tgagtttctg gaagtggtgt ttgagcagga tgcaaatttt
      361 tcgcgagcgg catttcacct cggcaccgga ttctccggtg ctcattgccg tgcgaagtgc
      421 gacttctctt cgtctcagtt cgatcgggaa gccttttttc tgttcgccat cttcgaccgt
      481 ccggctacgt ttgcctccgc gcgattcgga tcgcaggccg acttttccga tgccgcattt
      541 aaacaggccg atgatctcgc acaggcgacc ttcgcgcgca caccgcagct gaccagaacc
      601 gctcgtgtca gcacaacggt gccgggtcct acggcttcta cctcggctcc attttcccag
      661 gccgtcacga tcatcctatt tgtcatggcg ctggggctgt tggtctatat catcagggcc
      721 aagtagccct ggctgaaccc acaacatata gttttagtct tgcccggtcc ccccataaat
      781 tggtataaga ttgaaacttt ggccaaggga gcctctcgta cctgatggac caacctgaac
      841 tgccctacca agtcaagatc gagaatttcg aaggtcctct cgatctcctc ttgcacctca
      901 tcaagaaaaa cgaaatcaac atctacgata ttccgattgc gctgatcgct cagcagtatt
      961 tggactatct gtcggtgatg aaggaattga acctggctgt ggccggtgaa ttcctcgtga
     1021 tggcggcgac cctgctgcat atcaagtcac ggatgttgtt gccggtcgac gaggtggcgg
     1081 tggacgagga ggacggcccc gatccgcgcg aagaactcgt ccggcgactg ctcgaatata
     1141 agcagttcaa agaggcggct tcgcagctgg actataaaga acgtctatgg cgggatgtgt
     1201 tttcgcgcga gcccggtccg ccggtcgagg tcaagaaaga cgagagtctg ctcgacgaaa
     1261 tttccctctt tgatctggtt gatgcgctgc agggggtctt gtcccgccat ccaggcaagc
     1321 gacttgttga aatcattccg gacaacctca cggtccgcac ccgtatgagc gccattatcg
     1381 aggcgctgga actgcatgaa tccatggcct ttacggcgct gttcgaggag tcgagtcatc
     1441 ggcttgtggt gatcgtgacg ttcctggccc tgctcgaatt gattcgaatc agggtcgtgc
     1501 gcgtgtttca gagcgaaact ttcggggcga ttcttgtctc gcgtgccttc tcagcggttg
     1561 cggaagggga gctagtggag gagtctgaat ggaagaacct atgagtcccg gcgtggagga
     1621 ggcaatcgaa gaatcggtcg aggtgcggga cccggttgac gagccgactg catcgctcga
     1681 tgcggttgag gtggtgccgg agtcgggcca gggcgttgtc cacgacatgg cggccccggc
     1741 ggccgattct gcgctgtccg acatgcgcgc actgaagggt atcctcgagg ccttgctctt
     1801 tgtgactgcc gagcctattc cggtcacacg gttcttggca ttgctcggag cggttacgaa
     1861 gcaggatgtc gatcaggcgc tcgcgagcct tgctcaggac tatgagcagg aaggccgcgg
     1921 gctgcagttg gctgaagtgg ccggcggtta ccggatcgtc accaaggccg aattcgcccc
     1981 gtggctcaaa cggttggaga aggtaaagtc tccctcgaaa ctctcgcgct cagccttgga
     2041 atccctggcg atcattgcct acaagcagcc gatcgttcgc gcggaggtcg agcagattcg
     2101 aggggtcgaa acatcagggg ttattcggac gctgctggag cgaaaactcg tgcgcatcgt
     2161 tggccgcaag gaagagccgg gccgcccgat catgtatggg acgaccaagt ttttcctcga
     2221 acattttggg ttgcgcgatt tatctcagct gccgccgctt cgtgagttca aagagttggg
     2281 cgaatctgag caggcgatgt tgccgattga tgaggtcgtg gcagttgatg gagagcccgt
     2341 gacgcaatct acagaggcgg tcgtcgaagg gctgtccgaa ggcatggatg agcctgtggc
     2401 tcttcttgag ccggcagatg aagcggggca gacttcagag tcactcgttc tggagaatga
     2461 ggccggaggg gaccttgtag tcgccgatat cgcggaagag tcctgactgc tggtcgcaat
     2521 gtctgctcaa agactccgat agattcgccc cgcattgccg ccggcgcttt ttcagtcgtt
     2581 atgcataaaa ggccttcgag agactcctgt gaagggggtt ttcccgaagg ccttttgtgt
     2641 ctgacgatca gcagcagttc gcggctgcga gcacctagtg gcaaccgatt ggaacaaaac
     2701 ccgcgccgaa gatctgtgac agggtgacgt atcgggcgtt gtcatagaat gagtaactcg
     2761 gggtgtacca gtttcgggcc gtcggattgc tgccgtacga cggatcggtg ccgaagaagt
     2821 gaccgccacg gctgttctga ttcagatgaa tccattccat atacatgctg caaacttcgc
     2881 cctgcaccga cccgccgctg gcgttgcccg cataccagtc gcgtcctgac tcgggtatct
     2941 gggagagatt gcgcaaggcg ccgatggggg tgtcgtatga aaaatctttc gctttcgggg
     3001 tcggctgata ggtcgaaccg ttgggagggg cgagtggcag atcgggtagc gagggcacaa
     3061 cggagagcgc tttgaggacc ttggcctgac aatcgggtcg ctctttgttg gtgtagagga
     3121 tcgttccctc ggggcctggg cattcccaca tttctacagg ggatgactcg accgcgtgca
     3181 cgctgggagt aaggagcaga ctcgcggcga gggttgcgag cacaagtcgt gttggccaca
     3241 tggtacacct ccgattggtt catcttaccg ggcacgcgcg ttgttatcca acgtcgagag
     3301 tgcagagcct atgacattcg aaagaaatgc gtccatccag ctttgggggg atggatgcct
     3361 accctgccta gcgcgcggct tcgtacgctc tggtcggcgc ggtgactccg ttgccgatgt
     3421 atcagcggaa gagcctccag agcctcccag gccacgcggg tcttgccttg actcccttct
     3481 tttcgctttg ttagactgcc cgatctaagt tattgaaaca tccgccaatc ttcaccaatc
     3541 tcgtaccacg aggaacacca tgatcaaggc ttggctgctc gacgaaatgt ttcggtttga
     3601 ccggcggtat ctcacggcgg agggaagtca gagtgagtgg ggcgccgggg actccgttgc
     3661 tgaggaggaa gacctgcctc ctgcaccgac tggggtggcc gctcaagctg gaaatggccg
     3721 ggtgatgatt acctgggacg ctgtgccgga ggccatgtat tacaacctgt atttcatgac
     3781 caccaagggc gtgcagatta aattctccga actcactcgc cccatcgcca gcgcggaaga
     3841 tttcaaaacc gtcatcggcg tgaccaagga aaaggggaca tgtattgaag gtgcgcagtc
     3901 gcccttcatg catgacgatt tggcaaacgg aacgtgctac cactatgtcg tgaccgtggt
     3961 gacgcagaag ggggagagcc ccgaatctca ggaagtcatg gcgatcccgg ctccctacct
     4021 cattgccaag atcatcgggc aggagggcgt ggaggacggc gagttcagtt ctccgactgg
     4081 tattgcggta gacaaggacg gcaatatcta cgtggccgat accgataatc actccatcca
     4141 gaaattcgac aaagacggaa aatttctcgg tcgctggggc ggcgaagccg gcagcgcaga
     4201 aggcggcttc tattatccgc gcggactgac cacgaattcg gacgggcagg tgtacgtcgc
     4261 cgataccgga aacaatcgcg tgcagcgcct cgacaccgac ggcaatccca tgaaggcctg
     4321 gggtaagttc gggtttgcct ggcgtggggc cgacatgaat aagttcgacg ctccctgggg
     4381 tgtgaccacc gatcaggacg gcaatattta cgtgaccgat accaacaacg cccgcattca
     4441 aaagttcaag ggcgatggaa ccccgttgtt gaagtggggt cgggatggaa gtttcgacgg
     4501 ggcctttttc ttcccgcgcg gtgtggccgt ggacttcgtc gggaatacgt atgtggccga
     4561 tgaagggaat aatcgcattc agaagttcga tacccgcgga agctttttga ccaagtgggg
     4621 ccgtgaagga agcggtcccg gccagttcaa agccccctgg ggcgtgacct gcgatgcctt
     4681 gggcaatgtg tacgtcgtcg accagggcaa ccaccgtatt caaaagttcg acggaaacgg
     4741 gaccttcctc tgtgcttggg gaaatcgagg gaagaccgaa ggccagttga acttcccgtc
     4801 cggcgtggcc gtggacaagg aaggggcggt gtacgtcgtc gatagcggta accatcgggt
     4861 catcaaatac gtgccgaccg atgaagagct gaaccgcggc aaggcggaac gcgaacgggc
     4921 gcaagccgtc agtgagtacc cgattccccg caatctggcg atcaagcccg gagataccga
     4981 aacattcttg agctggatgg atgtacctgg ggcggtctcc tacaatctct acttccatac
     5041 ctcggcgaat ctgacgcagg aaggcggcac taagatcgag ggtgtgacca gcccatacaa
     5101 tcactcggga ttgaccaacg accaggccta ttattatgcg ctgacggcgg tctatgagga
     5161 cggcacggag agtggattgt cggacgaagt gtccgcgacg ccggtgctga tcgatatcac
     5221 ggctccgcag acgccgtatg cggtgatcaa ccacggcgcg tttatgacga actcgcctga
     5281 aattgtcgtc acgatttccg ccaccgacct cgataccggc gtggcggcgt actacatctc
     5341 agagaacccg atgaccccga tggcgggaac cccgggctgg gtggaagttc cgccggcgat
     5401 caagttcggc gcgacgattc ccttcattct gtctcctgga gacgggcaga agaccgtcat
     5461 cgtctggttc aaagacctcg gaaacaatat ctccacgccg gcgagtgcga cgattctcgt
     5521 caacacctcc ggctatcttt gtgtctccaa gtggggcaag cctggtcgag gcgcgtcgtt
     5581 gttgcacggc ggtgaattca tggcgccgat gtatgggctg gccatcgatc agcaggggtc
     5641 gatctttgtc gtggacaacg ggaacaaccg tatccagaaa ttcgatcgca cggggaattt
     5701 catcattctc tgggggaatt tcggcgccgc caatgcgaac ttccataacc cgacgggtat
     5761 tgcctgcgac gccaagggcg atgtctacgt ggtggatacc aacaatcacc gcgtgcagaa
     5821 gttcgacggg aagctgggcg gctatttgat gaagttcgga tcgcgaggga acggcgaagg
     5881 ccagttcaat gccccatggg gtatcgcgat cgatcgggtg cgcggataca tctatgtcgt
     5941 ggatagcgcc aacttcagag tgcagaagtt cgacatgagc ggagagttca tcatgtcatg
     6001 gggcagtttc ggcaacggcg acggtcaatt ctatttccct cgcgggatcg ccgtagatca
     6061 ggccgacggc acggtgtatg tcgtcgatat gggtaatcac cgcgtgcaga agttcgatac
     6121 cagcaccaac gtcttgccgc agttgctcac caagtggggc ggcagttccg aggccggcca
     6181 tgccagcagc ccgttggcgc aggaagccgg acagttgcgc tctccttggg gcattgccgt
     6241 cgatggtcag ggcgatgtgt acgtcacgga taccggcaac catcgtgtcg agaagttcga
     6301 ccgtgagggc aactttatcg ctcagtgggg tggattcggc ggtggtgacg gacaattcaa
     6361 cttcccgtac ggcatcgtgg tggattcccg ggggagtgtg ttcgccgttg atagcggaaa
     6421 cacccgggtg cagcaattca tgccggcgga tgaaggcagc gagcggttgc aggaagaggc
     6481 ggatgccgcc gcagagttag ggcaggtcga taaaaccacg agagtgtagg ggcaacgggc
     6541 gaaaggcccg ggagagggtg gcgactgatg ttagagaagg agattcggct cgggctcacc
     6601 tatgacgacg tcgtcttggt accggctaaa tcacaggtcc tcccgagcga ggtcgacacc
     6661 cgtactcgcc tgtctcgaaa cattcagctg aatattccca tcgtcagcgc ggccatggac
     6721 accgtgactg aggcgcgatt ggccattgca atggcgcaag agggtggaat cggcatcgtg
     6781 catcgtgtgc tctctcctac cgatcaggcg gctgaaatcg acaaggtgaa gaaatctgag
     6841 agcgggatga tcctggatcc cattacgatt tcgccggatc aaaccatccg tgatgcacat
     6901 gacctcatgg ccagataccg gatttccggt atccctgtga ccaagtcggg taaattggtc
     6961 gggatcctca cgaatcgaga cttgcgtttt gagacgcgaa tggaactgaa ggtgtcgcag
     7021 gtgatgaagc gcgataagct catcaccgct ccggagggaa ccagcctcga aaaggcccgg
     7081 gagattctgc acgagcaccg aattgaaaag ctgccggtcg tcaacaagca atttgacctc
     7141 aaaggcctga tcacgattaa ggacattgag aaacggatca agtatcccaa tgcctgcaag
     7201 gatgcccacg gtcgtttacg ggtcggggcg gctttggggg tgggacccga taccgcagac
     7261 cgggtggcgt tactggtgaa ggccggtgtc gacgtggtgg tcgtggatac ggcgcatggg
     7321 cattcgcagg ctgtgctgga tacggtcaaa atggtgagaa aagcacatcc taagttggac
     7381 atcattgccg gcaacattgc gacggcccag gcggccaagg atttggtcaa ggccggtgtg
     7441 gatgcggtga aagtaggcgt aggacctggt tcgatctgca cgacacgcat ggtgtcgggc
     7501 gcaggcatgc cgcagttgac ggcgattgca gactgcgcca gggcattggc gggatcgggt
     7561 gttccggtca tcgccgatgg ggggattaag tactccggcg atattacgaa agccttggcg
     7621 gccggtgcct cggtggtcat gctgggcagt ctgctggcag gaacggaaga ggctcccggc
     7681 gaaacggtgc tgttccaggc acgtacttac aaagtgtacc gcggcatggg ttcgatcggg
     7741 gccatggaac gggggggcgg cgatcgatac ggtcagggcg gacgaccgac accgaagttg
     7801 gttcctgaag gcattgaggg acgggtgccc tacaaaggca cgcttgctcc gcatatctac
     7861 cagatggtgg gcggcgtgaa gtccggcatg ggctactgcg gatgtaagac catcccggat
     7921 ttgcagcaga aggcgacgtt catccgccag acagtggccg gtctccgcga ggggcacgtg
     7981 cacgacgtca tcatcaccaa agaagctccc aactatcgca ccgattggga atagcctcgc
     8041 gtgaaacgtt tctcgtgaag cgtatctcgc aattcagatc ccctcactag acggacgacg
     8101 attcacgctt cacggaaaca tctccatgga actctggcac gatagaatcc ttgtccttga
     8161 tttcggctcc cagtacacgc agctgatcgc acggcgtatc cgtgaagcgc aggtctattc
     8221 gcagatcctg ccctgcaccg tgccgctcgc gacggtatta gcctaccgtc cgaagggtat
     8281 cgtgttatcc ggcggaccat cgagcgtgta cgacaaaaag gctccttcgg tatccaaaca
     8341 actcttcgaa cagggcattc ccattctcgg gatttgctac ggaatgcagc tggtgacgca
     8401 tctgcagggc ggcgaagtgg agaaggctcc gcaccgcgag tttggccggg ctgagctgac
     8461 cctcgatgac cggtcggatc tgttcaaggg aatagggact cggggctcta ccgtcgtgtg
     8521 gatgtcgcac ggggatcgca tcgaacggat gccgccagga ttccgctcca tcgcacacac
     8581 ggacaattct cccatcgccg cgatgaagcg gcacgacgcc aggcagcgca tctactgtct
     8641 tcaatttcat ccggaagtgg cgcacaccac cgagggcgcc acgatcctca agaacttcgt
     8701 ctacgatatc tgcggctgca agccgacctg gaccatgcgc tcctatgtcg acacggccgt
     8761 tgaacagatc aggcaacagg tcgggacgga gcgggtgatc tgcgccttga gcggcggggt
     8821 ggattcctct gtggcggccg cgctcacgca ccgtgcggtc ggcgatcaac tgacctgcat
     8881 tttcgtcgac aacggggtgt tgcgtaccgg tgaacgtgac caggtcgaga aaaccttcgc
     8941 gtcgcagatg catctgaaca tgcggatcct cgatcgcacc agtcagtttc tcacgggcct
     9001 gaagagcgtg accgaccccg agcggaagcg aaagattatc ggccgtctct tcattaagaa
     9061 ttttgaggtc gagtccaaaa agttgaaagg catcaaatac ctcgtgcaag gcacgttgta
     9121 tcccgatgtc atcgaaagcg tgagcttcaa gggcccctcg gcgacgatca aaacgcacca
     9181 caacgtcggg ggattgccga ccagaatgaa gttgaagctg gtcgagcccc tgcgtgaatt
     9241 gttcaaggac gaagtgcggg ccttgggcca ggagctcggc ctgccggatg agattatctg
     9301 gcggcaaccc tttcccggtc ccggcctggc gatccgcgtg ctcggcgcag tcaccaagga
     9361 acggctggcc attctgcgcg gggcggaaac gatcgtcgat caggaaattc gcgccgcggg
     9421 actgtatcga gagatctggc aggccttcgc cgtcctgctg ccgatccgga cggtcggtgt
     9481 catgggtgac cagcggacgt atgaatatgt cattgccatc cgcgcggtca cgagccttga
     9541 tggcatgacc gccgattggg ccaaaattcc gagcgatgtg ctggggaaga tgtcgagccg
     9601 gatcatcaac gaggtcaagg gcgtgaatcg cgtcgtgtac gacatcagtt ccaagccgcc
     9661 ttccacgatc gagtgggagt aacgcaagcg tgaggcgtca ttcataacgc gtcaggcgat
     9721 ggatacatat ccgatctaat ccctcgaatg acgagagacg cttcacagaa agtttgaatg
     9781 gaagtcagac tacaaaaact catcgccggc accggactgg cttcgcgacg caaagccgag
     9841 gagttgatca cgtccgggcg cgtgacgatc aatggtagcg tggtaaagga actcggcacc
     9901 aaagtcgatc ccgctcgcga ccatgtcaaa gtcgatggga aacatcttcg ggctgcgcag
     9961 ccctatgtgt atttgatcct caacaagccc aagaacgtca tgtccacgct ggatgatcct
    10021 gagggacgtc ccacggtgaa ggacctcctg cacggagtca ccgtgcgcgt gtttcctgtc
    10081 ggccgcttgg atttcgacag cgaagggctc atgttgctca cgaatcacgg cgacctggcc
    10141 caggcgctgc tccatccccg ctaccatgtt ccgaaaacct atttgatcaa ggtcaagggt
    10201 gtgctcgacg atgcgaagat cgaagctctt gaacgaggcg tcaagctgga agacggcttc
    10261 acggcgccgg cgaaggtgaa taaggtcagc aaggctgaga gcaattcgtg gcttgaggtc
    10321 acgattcacg aaggacggaa acatcaagtg aagcggatgg tcgaggcggt gggccattcg
    10381 gtgattaagc tcgtgcgtgt ccgcatggga ccgttgcttt tgggagattt ggcgccgcga
    10441 gaatatcgat tcctgaccga ccgcgaggcg aatcggttac gggatctggt ggaggatcgg
    10501 gtggccagag cggaacgtcc ggaactggac gcgtccggaa aaccgactcg atggatgccg
    10561 ccgacggagc cggctccgcc tcgtcctccc aagcccgagc gttcagggcg tgggccaaaa
    10621 gctcagcgtt ccgaacgggg gccgcgatct caacggtctc tggccagtgc gaaaccttca
    10681 cgctcaggtc gcggaaccac gcggcggccc atgagtccag gtgccaaatt tcgtcgtcct
    10741 gccaaagggg ttggttttca acggactcag gctggtccga tcccgcaggg ggctgatcgt
    10801 ggccctggct cgcgtccgac cgggccagac caaaaacccc agcgcactca gcctggagca
    10861 ggatttcagc ggccccggtc cggtgcgaaa cctcaaggcc ccggtcgggg catgacggtt
    10921 cggcgctcgg gtcctggtca gaactttcaa cagaagcggc gcggcggatc gtcagcttcg
    10981 cgcccgggac aacgaggccc tgctggaaaa tcgcggccct caacgcggag aagagcatga
    11041 agatcaggac ccatcggtgt ggggaattga ccaaggcgca agtcggtcag caagtggtgt
    11101 tgaacggatg ggtgcaacgt cgccgggatc acggcatggt gctgttcatc gatctgcgtg
    11161 atcgaacagg gttgacgcaa gtggtgttca acgccgagcg gaatgcggcg gtccaccaag
    11221 ccgcacatac cttgcggagc gaatgcgttg tgtcggtgac cggccaggtg atggccaggc
    11281 cggatgagtc caagaatccg aacctgccca ccggtgagat cgaagtgttc gtggatgccg
    11341 tcgagattct caacgaagcc aagacgccac ctttcatgat cgaggacgac attgagatca
    11401 ccgagtctct tcgattgaag taccgctatc tggatctcag gcgtccgagg atgcagcggt
    11461 tgctgggatt gcgtcacggg atcatgcagg cggtgcgggc attcatgaat gcggagcagt
    11521 ttctggaagt ggaaacgccc gtgttgacca aaagtacgcc ggaaggcgct cgtgattatc
    11581 tggtgcccag ccgcgtgaat ccggggatgt tttatgcact gccccagtcg ccgcagctgt
    11641 tcaagcaggt gttgatggtg agcggtgtgg atcggtatta ccagattgcc cgttgcttcc
    11701 gcgacgaaga ccttcgtaac gatcgccagc ctgaatttac ccagattgac ctcgaaatgt
    11761 cgttcgtgga tcgtgagcag gtcatgacgc tcatggagca catgatcgtc tctgtcttca
    11821 aggatgccgg cggggtacag ttgcccacgc cctttccccg gatgacctat gccgacgcca
    11881 tggggcgcta cgggtcggac aagccggacc ttcgcttcga catgccgttg catgatgtca
    11941 cggcgtttgc cgcgaagagc gaattcaagg tgttcaagga ggcggcgacc aagggcggac
    12001 tcgtcaaggc gctgatcgtg tccggagggg cctccattgc gcggagtcgt atcgacgcgc
    12061 tcggtgaaac ggcgaaaagt tttggcgcga aaggcctggc ctggttgaaa gtcactgcgg
    12121 acggccagtt agaatcggtg atcgccaaat ttctcgatgc caaggccttt gccgaggcct
    12181 tgccggaagc gaagcccggc gatctggtgc tcttcggcgc ggataaaccg gccatcgtgc
    12241 acgatgtctt gggacgaatc cggctgctgc ttggtgagga actgaatctc atcgacaaaa
    12301 cagcctggaa gcccttgtgg gtgacggagt tcccgctgtt ggactattcg ccggaagaga
    12361 aacgctatat cttcatgcac aatcccttcg ccgcgccgat ggatgaagac ctgccgttcc
    12421 tcgacacgga gccgttgaag gccagggcca aggcctacga catggtgctc aacgggagcg
    12481 aaatcggcgg tggtagcatt cgcaatcatc gtagcgacat ccagttgcgg attctggatc
    12541 tgttggggat cggcaaggaa caggctcaag cgaagttcgg cttcctgctg gaagcgctgg
    12601 agtatggcgc gcctccgcac ggcggcattg cgttcggcct cgaccggttg atcatgctgc
    12661 tgggtggcgc cgattcgatt cgcgatgtca tcgcgtttcc caagacgcaa cgcgcgcaat
    12721 gcccgctgac cgaagcgcct tccggcgtga gtgccgagca attgaaggag ttgcgcatca
    12781 agttggatct cgtcgagtag gtaggagtca tccaacccat ggccggcaat acgttgggcc
    12841 gagtcttcac cgtcacgtca ttcggcgaaa gccacggacc ggcgatcggc tgcgtggtgg
    12901 atgggtgtcc gccaggcctt gcgctatccg ccgaagacat tcagcgggat ctcgaccggc
    12961 gcaagccggg cacctcacgc cacgtcacgc agcggcagga atccgatacg gttgaaatcc
    13021 tttccggggt gttcgagggc aagacgaccg gtacgcccat cgccctcttg atccggaacg
    13081 aagaccagcg cagccgtgac tatggcaacc tcctcgacac ctttcgaccg ggccacgccg
    13141 actacaccta ttggcagaag tacgggatcc gcgaccatcg cggcggggga cgatcttcgg
    13201 cgcgtgaaac ggccgtgcga gtggcggcgg ctgcgattgc caagaagtgg ttgcaggaaa
    13261 agcacggtgt tgtcatccgt ggctacctca gtcaactcgg gccgatcgaa gctccctttc
    13321 agagctggga aacggtggac accaatccat ttttttcggc gaatgccgag gtggtgggga
    13381 agcttgaatc gttcatggac gagttgcgga aggccggcga ttccgtgggc gcaaagatta
    13441 ccgccatggc cgagcatgtg ccggtcgggt ggggtgctcc ggtctatgcg aagctggatg
    13501 ctgacctggc cggcgccatg atgagcatca atgcggtcaa ggcagtggag atcggcgcag
    13561 ggttcgcctc ggtcgcgcag cggggttcgg agcatggcga tgaactcacg cctgaagggt
    13621 ttgtgacgaa tcatgccggc ggcattctcg gcggtatttc caccgggcag gatgtggtcg
    13681 tgaccatcgc catcaagccg acatcaagca tccgtattcc gcgacgttcc atcgacaagc
    13741 agggcaaccc ggtgaccgtc gaaaccaatg ggcgtcacga tccttgcgtc ggtatccggg
    13801 ccacgccgat tgctgaagcc atgatggccc tggtgctgat ggatcacgca cttttgcatc
    13861 gcgcgcaaaa cgccgatgtg cagacggcga cgccgaagat tccaggttct tcaacgcatg
    13921 gtgctgttcc ggcgagtaag aaaccaaccg catagatcaa ggttgattct gcgaagccga
    13981 gtcgtcttcg gtctgactcg tttgtttgat cggcgggcta gggggcgttc gttatacctg
    14041 atgatgaaca gtagaggact ctgagccaat gatcgaaatt tataccgacg gggcctgcag
    14101 cgggaaccca ggacctggtg gctggg
//
LOCUS       DAOQRM010000059        14030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3409_length_14030_cov_15.788050, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000059 DAOQRM010000000
VERSION     DAOQRM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14030
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3409_length_14030_cov_15.788050"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKK81_06565"
     CDS             complement(<1..273)
                     /locus_tag="PKK81_06565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV32571.1"
                     /translation="MKAGRSLLLGMLLAVGGQLMGAGAYAQGDLAIADGVKVSLEYIL
                     TLPDKSVADSNVGQEPIIFVQGAHEIVPGLEKALDGMKAGQKRRIEV"
     gene            complement(330..1682)
                     /locus_tag="PKK81_06570"
     CDS             complement(330..1682)
                     /locus_tag="PKK81_06570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV32572.1"
                     /translation="MVSFAELSLTSFLADRLQQAGFTAPTPIQSAAIPLALEGRDLLA
                     QAKTGSGKTLAFLIPLIERAVKEGWKPAGHASASHAGSSRSPRALVLAPTRELALQIE
                     MELRKYAPPSVTSLAVYGGVPIERHYRALRQPPLIVIGTPGRLLDVAGTRHLDLRGVE
                     YVVMDEADQMLDRGFLRDIQRILQLLPTQRQTMLFSATFSPEILTLAESMLKNPARTA
                     VDPGVNTPTTITHAYYVVPSESSRVQLIHTLLQSPESGDQSMVFCDQKYKVKRLAARL
                     GGEPASVGAITGNHSQAQRERTLTAFRSGRLRTLVATDVAARGLDVPSVSQVIHYELP
                     GNPTSYVHRTGRTGRAERSGATLLILSPQEEHEYLAMVRRLRIQTKRLTLPALAVLPP
                     PAHEPESNGQSRHDGRGRDARPRRAGERQNSMPQDARGGQGRRGWRADRPAAPRRGNS
                     "
     gene            complement(1831..2130)
                     /locus_tag="PKK81_06575"
     CDS             complement(1831..2130)
                     /locus_tag="PKK81_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNV32573.1"
                     /translation="MGSKIYVGGLPYSTTEQQLSDLFAVHGAVTSARIITDKFTGQSR
                     GFGFVEMSGDSEAQAAINALNGTQFGGRTLTVNEARPQEPRSGGGGGRGMGGGRH"
     gene            complement(2407..3237)
                     /locus_tag="PKK81_06580"
     CDS             complement(2407..3237)
                     /locus_tag="PKK81_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32574.1"
                     /translation="MQYGHELVRQIEFLRDSQSLAMAHLRNIRRYAGVIVLTLMSVSG
                     VESVLAVPKETSTEKAEHLRQQANPQLFNNWTFDKDQAGGVPKGFLAVASGDQPGGDW
                     KVEAQSAAPSSPNVVLGASSCPACVELLVAQEFQYEYPELVVRIRQGADATSGQIGVV
                     FGLKDPKNYYATVVDLSQKSIEVVRVIEGKESILGRAAIKSKPVEWHTLRVQRNTIIS
                     KDFVETFFDGSLALSVEDQTLGVGQVGILVRGQATARFDNFNASPLYSSRPLSAPAAY
                     "
     gene            complement(3264..3509)
                     /locus_tag="PKK81_06585"
     CDS             complement(3264..3509)
                     /locus_tag="PKK81_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32575.1"
                     /translation="MAEQGKGLYDHQYGRFRENQGTHQGYEFQHGPAGSVPPPVVTFM
                     DKIKWWTLRGWRRKKILAERDRFQQDIIRIKQTQPKS"
     gene            3707..4144
                     /locus_tag="PKK81_06590"
     CDS             3707..4144
                     /locus_tag="PKK81_06590"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNV32576.1"
                     /translation="MDLSQQPPRRWSDTLAGIMWLPRLIDKVRAFQAGTLGTYAYPSA
                     LDQSFMRRFQLTPAYIEPLVREAASDEAIGTAIRARIQLSDEEVQHRCAIFRDKYRLA
                     FAVLDRDDGYVRGLGYPIPRFLQPPLWRWYQRWSAQKASATSI"
     gene            4252..5736
                     /locus_tag="PKK81_06595"
     CDS             4252..5736
                     /locus_tag="PKK81_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNV32577.1"
                     /translation="MALAAGQSIAAETGAPSTTLTQALSQISSERMLADIRALSGPAF
                     NGRQTGTPDDLASAEFVRQRFLELQQHRSPGSESPAPPDRLPAHTQIQSAPVRITQID
                     DDSLLQIGGIAESQPSVVGTDYLPILDSPSAELETSVVFAGYGISDQAGGLDDYAGID
                     VRNKVVLFLRGKPERYAKQVSHADKVHMAHAHGAIGYLTATGPILNAYETRRGVTGRP
                     SAFYGLTDPHRTIPGAWISTALASAILNAHQPGEDNRLRRLQQQLNDTMAPQSTTTDV
                     SIEVRWRSTQQNGTLQNVVALLPGQDAAHQHDAIVIGAHRDHFGKQGGLLFAGADDNA
                     SGTAVILEVARVLTSMPVGPKRSIIVVSFSGEEQGLLGSKLYVTQPMVPLTATAAMIN
                     VDHAAVGNGRLTIGVTGLEKPAAQQAGERAGLADRIDLFGFFPGGDHVPFKEAGVPTV
                     TVVSGGIHPHFHQPTDTADTVNADILSAAARYILAIAWQLADAP"
     gene            complement(5826..7991)
                     /locus_tag="PKK81_06600"
     CDS             complement(5826..7991)
                     /locus_tag="PKK81_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNV32578.1"
                     /translation="MEEIISVNDQFYILASSSMADDRTRVLKHGETFGVYDRYGDIQP
                     VGRGTQGVFHQGTRFLSRQELFLNNDRPMLLSSTVKEDNALLAVDLTNPDLYRDGRIA
                     IPRGSVHVFRSRFLWNGVSYERFRLSNYSLAPVKMTLSIRFEADFADIFEVRGKKRER
                     KGRMLPNVLRKDLLVLRYEGLDEVTREARIQFSPEPLEITASQATFDIELDPKGETTV
                     AVMVACDVGDSHRPLLAYDAAMAEAGLAFGAEQTEDCTIQTSNAQFNEWWNRSVLDVR
                     MMVTDTNEGPYPYAGVPWFSTPFGRDGVITALECLWIRPELARGVLAYLASTQAKEVN
                     PGQDAEPGKILHETRKGEMAALNEIPFGLYYGSVDSTPLFVMLAGAYYERTADLAFIQ
                     SIWANLEAALTWMDTFGDPDRDGFVEYVRKSPTGLDNQGWKDSHDSISHEDGSLAEGP
                     IALCEVQGYVYDAKVQASKLADALGYIDRASQLRRQARSLKERFEEAFWCEESSTYAL
                     ALDGQKRPCQVKTSNAGHCLYTGIASDEHARRVAETLMSDELFSGWGVRTLADSERRY
                     NPMSYHNGSIWPHDNAMIAVGLARYGLKSGVEKIMTGMFEVSLVLDFHRLPELFCGFV
                     RRPGQGLTRYPVACNPQAWAAGSAFMALQACLGLSIIASEQKVVFNYPILPEFIDKMQ
                     IKNLKVGTASVDLLLRRHDLDVGITVIRRAGKVEVVSVK"
     gene            complement(8143..9468)
                     /locus_tag="PKK81_06605"
     CDS             complement(8143..9468)
                     /locus_tag="PKK81_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV32579.1"
                     /translation="MNGGGTGRPNVKWSPHLLTRDFTLVWWGQMVSQVGDGVSKLALL
                     WFVYSITGSPLKTTMIGLLQTLPPILFGPFIGVIVDRVPKKLLLISSDLIRALVLGVL
                     PCLLPVDSFSIERLYLMVFVHAVASAVFGPALTAAIPSLVSRHEFTAANALLQTTTSI
                     GIIVGPALSGVGIATMSSQEVLCVNAVSYVISAACFAFIRFPRAESQPVGGGSLAGTF
                     SDVLDGFHYVLHRQRVILMLIGAASMYTFATSAFSTLFPVFGKKLLDLGPIEVGYLWS
                     AFGIGLLLVSLGLVSLSAWSLPKRIQLMALSSFISGLALLGLIVTSNRLIAAALMVII
                     GMGAGTLTPVAWGVLQEIAPASLLGRVLAIYNLGAMTSAIAGMTIFGWVTQDFGERPS
                     VFGIGVGLFLSALVSMRVARWVQTNWAGATVPSEEESQSVVMVTQPTSH"
     gene            complement(9538..10542)
                     /locus_tag="PKK81_06610"
     CDS             complement(9538..10542)
                     /locus_tag="PKK81_06610"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNV32580.1"
                     /translation="MPMRVIRTVYVTGVLALLAASGVGNVNGTALAESTPEGKSETPP
                     VQRPKDPDTKPKEPEPKTKEADSKPKESDAKPPEKPKEAEAKPKEPESKVKEPETKAK
                     DQEPKAKEPAAKAKESEHKVKEVEPKSKEPESKAKEPDAAAEHPCPTVPHAAEVKPAA
                     ETQAPPGEPGAGERPKAAEPEGKKPIRSSMVTAKLALMADPHLFPYDIEVDAKDKDLV
                     LLGKVSQESDKRVATDIVRCLDGVHAVENRLKVEQDAAHGLFAERDKIITQLVKERFE
                     KSKTLQSVKFDVKTEDGIVTLNGATRFQIIVLEAAQAARQVPGVRAVNTDAVRLVAGE
                     "
     gene            complement(10777..12057)
                     /locus_tag="PKK81_06615"
     CDS             complement(10777..12057)
                     /locus_tag="PKK81_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV32581.1"
                     /translation="MAAESQSTPESNVDGWRLLGTRDFGCLWAGQVVSQIGDGLNKVA
                     LLWFVYEMTGSALKMTAIGLLQTIPPLLFGPLIGVYLDYLPKKTVMIVVDFLRALMVL
                     LIPLFYTFDMLTLERLYVLVFLISIVSTVFGPALASAVPLIVQRSQLTTANAFLQSTT
                     NIGVLLGPAMSGLGIALIGAQNVLYVDAATFLVSALFLLPIRVRDSRTVKGLDVLATP
                     VMQDMMVGFRFVFLQHRVVFALMITAVLYNLAISAFVFLLPVVAKELLQVGPMELGWL
                     WSALGIGMLAASIWLARTPQGTFQDRIGKIGRSLAIGGIAVCALGLIQTPVLFSTFLL
                     IVIIGGSTSLFYPVVWAMLQEVTPEHLLGRVFTTFSVGGMASAMVGMAGFGWAADTLG
                     PAVSLIGIGLLLLLTAMVTVQVSRRGHVVSPAVA"
     gene            complement(12091..13164)
                     /locus_tag="PKK81_06620"
     CDS             complement(12091..13164)
                     /locus_tag="PKK81_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV32582.1"
                     /translation="MRIAQVSPLWESVPPKLYGGTERIVSYLTEELVRQGHQVTLFAS
                     GDSVTRAKLEAPCQQALRLNTGIFNREAPLIQMMEQVFASADQFDLIHSHLDFLAFSL
                     SRRCRVPVVTTLHGRLDLPELVPVFRDFAELPLVSISDSQRRPLPWCNWQNTVYHGLP
                     HDLYKFHPEPGKYLAFLGRVSPEKCPDQAIELAKRVGIPMKMAAKVDPADRAYFERVV
                     EPLLDHPLIEFVGEITDAEKSDFIGNAIGLICPYDWPEPFGLVLIESLACGTPVLAYR
                     RGSIPEIIGHGVTGFISENLDEMVSQVDKLGTIDRNRCRQMFDERFTAQRMTTDYVKI
                     YQQLITDAAALPGKSGQQHASNL"
BASE COUNT         3030 a         4487 c         3866 g         2647 t
ORIGIN      
        1 cacctcgatg cgccgtttct gtccggcctt catgccgtcg agagcctttt cgaggccggg
       61 gacaatttca tgcgccccct gcacgaagat gatcggctcc tgaccgacat tcgaatcggc
      121 gacggacttg tcgggaagcg tcagaatgta ctccaacgac actttcaccc cgtcggcaat
      181 ggcgagatcg ccttgcgcgt aggcgcccgc ccccatcaac tgacctccga cggccagcag
      241 catgcccagc aacagactcc gtccagcttt catcgacgcg tcctcctaat catgccggtc
      301 cagcaatccc tgccgaacca tgcggttctt caactgttgc cacggcgcgg ggcggcggga
      361 cgatccgcac gccaacctcg acggccctgc ccgcctctcg catcctgagg catggagttt
      421 tggcgctctc cggcacgtcg cggtcgggca tcgcgccccc gcccatcatg cctggattgg
      481 ccattcgact caggctcatg agcaggaggc ggcagtaccg ccagtgcggg caacgtcagg
      541 cgcttcgttt ggattcgcaa acggcgcacc atcgccaagt attcatgctc ttcctgcgga
      601 gagagaatca agagcgtcgc gccggatcgt tcagcccgac ccgttcggcc ggtgcgatgc
      661 acgtaggacg tggggttgcc gggcaattcg taatgaatga cctgcgacac cgacggcaca
      721 tccaatccgc gagcggcgac atcggtcgcg accagggtgc gcaaccggcc cgatcggaac
      781 gccgtgaggg tccgttcccg ctgggcctgc gagtggtttc cggtgatggc accgaccgag
      841 gctggctctc cgcccagacg ggcagccaac cgtttcactt tgtatttctg atcgcaaaag
      901 accatcgact gatcaccgga ctccggagac tggagcagtg tgtgaatcaa ctgcacccgc
      961 gaggattcgc ttggcaccac gtagtacgcg tgggtaatcg tggtcggcgt attcacgccg
     1021 ggatccaccg ccgttcgagc cggattcttc agcatcgatt cggcgagcgt cagaatttcc
     1081 ggcgagaagg ttgccgaaaa cagcatggtc tgtcgttggg tgggaagcag ttgcagaata
     1141 cgctgaatgt cgcgcaggaa tccgcgatcc aacatttgat ccgcttcatc catgaccaca
     1201 tattcgaccc cgcgcaggtc caaatggcgg gtccccgcca cgtccaacaa acgccccggc
     1261 gttccaatca cgatcaacgg cggttggcgc aaggcgcggt agtgccgctc gatggggaca
     1321 ccaccgtaca cggccagcga cgtgactgac ggcggcgcat acttgcgcaa ttccatttca
     1381 atttgaaggg ccagctcacg ggtcggcgcg agcacgagcg cccgcggcga tctggaagat
     1441 ccggcatgcg acgcggaggc atgaccggca ggcttccagc cctctttgac ggcccgttcg
     1501 atcaagggaa taagaaaggc cagcgttttc ccgctgccag tcttggcctg ggccaacaaa
     1561 tcgcgcccct ctaacgccag cggaatcgcc gcgctctgaa tgggagtcgg cgcggtaaac
     1621 ccggcctgtt gtaaacggtc agcgagaaac gatgtaagag acaattcggc aaacgacacc
     1681 atgcaacgac actcctaaat ctcaatcttg ggaaaacagg ggcagataac ctgatggccc
     1741 tgcagaccag agagaggaca acacggtaag aaaaacgcag gggctaccct gtcggcagcc
     1801 cctgaatagg tatagcgaaa cgagcgcagc ttagtgccgg cccccgccca ttccacgtcc
     1861 tccgccacca ccggagcggg gctcctgcgg gcgggcttca ttcacagtca acgtgcggcc
     1921 cccgaattgt gtcccgttca acgcgttgat cgccgcctgc gcctcggaat ctcccgacat
     1981 ttcgacgaag ccgaagcccc gtgactggcc ggtgaatttg tccgtgatga tgcgcgccga
     2041 cgtcaccgcc ccgtgcaccg caaacaggtc actcagctgt tgctcggtgg ttgaatatgg
     2101 caagccgcca acgtagatct tcgaacccat tgggttcctc ctttgagaat aaaatggtgt
     2161 tgtctgggac tcgagaaaga aacgaggaag gaatggggcg aagatgcaaa cacagcggca
     2221 atcttagctc tggcttccga aattcccggg aagaacccaa aacaacatcg aaatcgagcc
     2281 ctgtaattct ttttctactt caccgccaag gctcttcgca atgaagcacc ttcactgtat
     2341 cctaccgaaa taggaaaggc aagcgtgaaa tggtctccga gtcgagcagg cccgccggca
     2401 tcgcgcctag taggccgccg gcgccgacaa gggacggctg gaatacaatg gtgaagcgtt
     2461 gaaattatcg aatcgcgcag tcgcctgccc acgcaccaga atccccacct gtccgacgcc
     2521 aagcgtttga tcctcaacag aaagcgccag gctcccgtcg aagaaggtct ccacaaaatc
     2581 cttactgatg atggtgttcc gttggacccg gagcgtatgc cattcaaccg gcttcgactt
     2641 aatcgccgcc cggcctaaaa tcgactcttt gccttcgatc acccgcacca cctctatgga
     2701 cttctgcgac aaatccacca cggtcgcata ataattcttc ggatctttca ggccgaacac
     2761 cactccaatc tgcccggacg tggcatcggc tccctgacga atccggacga ccagctcagg
     2821 gtactcatac tgaaattctt gcgcgaccaa caactcgaca caggcgggac aggaagatgc
     2881 ccccagcacg acattgggcg aggaaggtgc cgccgattgc gcttcaactt tccagtcccc
     2941 gcctggttga tcgcccgaag ccacggcaag aaaccccttt ggaacccctc ccgcctgatc
     3001 cttgtcgaac gtccaattgt tgaagagctg gggattcgcc tgttgccgca aatgctccgc
     3061 cttttccgta gacgtttcct tcggcaccgc cagcaccgat tccaccccgc tcaccgacat
     3121 cagggtcaac acgatgacac cggcgtatct cctgatattc ctgagatgcg ccatagcaag
     3181 cgactgactg tcacgtagga actctatctg ccttaccagc tcgtgtccat attgcacggg
     3241 agcactcctc cttcgagcgg atcttacgat ttcggctggg tctgcttgat ccggatgatg
     3301 tcctgttgaa atcgatcgcg ttcagccagg atcttctttc gtcgccagcc gcgcaacgtc
     3361 caccacttta tcttgtccat gaacgtcacc acggggggag gcacgctccc cgcaggcccg
     3421 tgctgaaatt catagccttg atgcgtacct tgattctcgc gaaaccgtcc gtactgatgg
     3481 tcgtacagac cctttccctg ctctgccata gatcctacct cttgcctcgc ttggtgatcc
     3541 cgacctgccc tcacggagga tcagctcctg aaggaacggc cccgacgctc acctgatggc
     3601 cgatagtaca ttgagccctc ggctctctgc aatgggatcg accgggtatg ctatagtgcc
     3661 tacggttcgc aagaaacctc tcctacccag gattatgagg caccgcatgg acctatccca
     3721 gcagccgccg cgacgatgga gtgacaccct ggcaggcata atgtggctgc cacggttgat
     3781 cgacaaggtg cgcgcctttc aggccggcac gctcggaacc tatgcctacc cctccgcact
     3841 ggaccagtcc ttcatgcgcc ggttccagct gacgcctgcc tacatcgaac cgctggtccg
     3901 tgaagcggca tccgatgagg ccatcggaac cgcgatccgg gcgcgcatcc agctgagcga
     3961 cgaggaggtg caacaccgct gcgcgatctt ccgtgacaag taccggctgg cattcgccgt
     4021 gctggaccgg gatgacggct atgttcgcgg cctggggtat ccgatccccc gctttctgca
     4081 gccaccgctc tggcgatggt atcaacgctg gtcggcacaa aaggcctctg ccacgtctat
     4141 ctgatgccct gcgatcgctc cataccactt gcgccttgcc ggcgagacag caggcgcact
     4201 gaatcgtcat acattgatcg actcccgttc tacctgctgt tcatcttata tatggccctg
     4261 gccgccggcc agagtatcgc cgcagagacg ggcgccccgt ccacgacgct gacgcaggca
     4321 ttgagtcaga tttccagtga gcgcatgctg gcggatatcc gcgcgttgag cggaccggca
     4381 ttcaacggac gccagaccgg gacgcctgac gaccttgctt ccgcggagtt cgtgcggcaa
     4441 cgattcctgg agctacaaca gcatcgctca ccgggctccg agtctcccgc cccgcccgat
     4501 cgactccccg cgcacaccca gattcagtcc gcccccgtcc gcataactca gatcgacgat
     4561 gattcactgc tccagattgg agggatcgct gagagtcaac cttccgtggt cggcaccgac
     4621 tacctaccaa tcctcgattc cccttcagca gagctggaga cctcggttgt ttttgcggga
     4681 tacggaatct ccgatcaggc cggcggactc gatgactatg cggggatcga cgtccggaac
     4741 aaagtcgtgc tgtttctccg gggaaaacct gaacggtatg ccaagcaggt ctcccacgca
     4801 gataaagtgc acatggccca cgcacatggc gccatcggct acctcaccgc gacaggaccg
     4861 atcctcaatg cctatgaaac ccgccgtggg gtcacgggtc ggccgagcgc cttctacgga
     4921 ctcacggacc ctcaccggac gattcccggc gcatggatca gtaccgctct cgcctcagct
     4981 atcctcaacg cacaccagcc cggcgaggac aaccggttgc ggcgccttca gcagcaactc
     5041 aacgacacca tggccccgca gtccacgacc accgacgtct cgattgaagt gcggtggcgc
     5101 agtacgcaac aaaacggcac gcttcagaac gtcgtcgcac tccttccggg acaggatgcc
     5161 gcacatcaac acgacgccat cgtgatcgga gctcaccgcg atcattttgg aaaacaaggc
     5221 gggctgctct ttgccggagc cgacgacaac gcatcaggca ccgccgtgat cctggaggtc
     5281 gcccgggtac tcacctccat gcccgtcggc ccgaaacgtt cgatcatagt ggtgtcgttc
     5341 agcggagaag aacagggctt gctgggatcg aaactctacg tgactcaacc aatggtgccg
     5401 ctgaccgcta ccgcggccat gatcaatgtg gatcatgcgg cagtcggaaa cggacggctc
     5461 acgatcgggg taaccggttt agaaaaaccc gctgcgcagc aggccggaga acgggccgga
     5521 cttgcggacc gtatcgatct gttcggtttt ttcccggggg gagaccatgt gccgtttaag
     5581 gaagcgggag ttcccaccgt aaccgtggtg agcggcggaa tccatccgca cttccatcag
     5641 ccgactgaca cggcggacac cgtcaacgca gatattctct cagccgccgc gcgctacata
     5701 ctcgccatag cctggcaact cgccgacgcg ccctaacact tcagcggctc actggccgtt
     5761 ctgcccccaa ccggatgtta tcgagccggc gctcacaccg cggccgagcc ttcacgcagg
     5821 cgccgttact tcaccgacac cacttcgact tttccggcac gacgaatgac cgtgataccc
     5881 acatcgaggt catggcgccg aagcagtaaa tcgaccgatg cggtccccac cttgaggttc
     5941 ttgatctgca tcttgtcgat gaactccggc aggatggggt aattgaacac cactttctgc
     6001 tccgacgcga taatcgatag gccaagacag gcctgtagcg ccatgaaggc cgagccagcc
     6061 gcccaggctt gagggttaca ggccaccgga taccgcgtca ggccctggcc gggccggcgt
     6121 acgaatccgc agaagagttc cggcaagcga tgaaagtcga gcacgagact gacctcgaac
     6181 atgccggtca taattttttc cacaccggac ttgaggccgt accgggccag accgaccgca
     6241 atcatggcgt tgtcgtgcgg ccagatcgat ccgttgtggt aggacatcgg attgtaccgg
     6301 cgctccgaat cggcgagggt ccgcacaccc catccgctga acagttcatc ggacatgagc
     6361 gtttccgcca cacgcctggc atgctcatcg cttgcgatcc ccgtatagag acaatggccg
     6421 gcgttggaag tcttcacctg acagggccgt ttctggccat ccagggcaag cgcataggtt
     6481 gacgattcct cgcaccagaa tgcctcttcg aaacgttcct tgagcgaccg cgcctgacgc
     6541 cgaagctgac tggcacgatc gatatagccg agcgcatctg ccagcttcga cgcctgcact
     6601 ttggcgtcgt acacataccc ttgtacttca cacaaggcga tcggtccttc agccagcgaa
     6661 ccgtcctcgt gcgaaatcga atcatgggaa tctttccagc cctgattgtc caacccggtg
     6721 ggtgatttcc gtacatattc tacaaacccg tcacggtcgg gatcgccgaa ggtgtccatc
     6781 caggtcagcg ccgcttccag gttggcccat atcgactgaa taaacgccag atcggccgtc
     6841 cgttcgtaat aagcgccggc cagcatcaca aacagcggcg tggaatcgac actgccgtag
     6901 tagaggccga agggaatctc gttcaacgcc gccatctcac ccttgcgggt ctcgtggagg
     6961 atcttccccg gctcggcatc ctgaccaggg ttcacctctt tggcctgcgt cgatgccaga
     7021 taggccagga cgccgcgcgc caattccggc ctgatccaca gacactcgag cgccgtgatg
     7081 accccgtccc ggccgaaggg tgtgctgaac cagggcacgc cggcataggg ataggggccc
     7141 tcattcgtgt cggtgaccat catacggaca tccaacaccg agcgattcca ccattcattg
     7201 aattgggcgt tggatgtctg aatcgtgcaa tcctcggtct gctccgcacc gaaggccagg
     7261 cccgcttcgg ccattgccgc atcgtatgcc aacaggggcc gatggctgtc ccccacatcg
     7321 caagccacca tcaccgccac cgtcgtttcc cccttcgggt cgagttcaat atcgaaggtg
     7381 gcttgtgagg ccgtgatctc cagcggctcc ggagaaaact gaatccgcgc ctcccgggtc
     7441 acctcatcga ggccttcgta cctcaacacc agcaggtcct tccgcagtac atttggcagc
     7501 atccgcccct tccgctcacg cttttttccg cgcacctcga agatatccgc aaagtcggcc
     7561 tcaaaccgga tggacaacgt catcttcacg ggcgccagac tgtagttgga cagtcggaag
     7621 cgttcgtaac tcaccccgtt ccaaaggaag cgcgaccgga atacgtgcac actcccgcgc
     7681 ggaattgcga tccggccgtc ccgatacaag tccggattcg tgaggtccac ggccagcaag
     7741 gcattgtcct ccttgaccgt tgagctgagc agcatcggcc ggtcattatt cagaaacaat
     7801 tcctggcgcg acaaaaaccg cgttccctgg tgaaagacac cctgggttcc ccggcccacc
     7861 ggctgaatat ccccatatcg atcgtagacg ccgaacgtct ccccatgctt gagcacgcgc
     7921 gtgcgatcgt ccgccatcga tgaactggcc aggatataga actgatcatt gacactgata
     7981 atttcttcca cgtcttcctc cgctgctccc agaatcaccg acgcgacgag tcacgaccga
     8041 catcacgacg ccctcgtccc gttttttcgc ctactcaagc tgcgcctcgt ccggctcccc
     8101 ttccagagag ggccgatccc ctcgacctcc cgctgaatgc tattaatgac tcgtgggctg
     8161 tgtgaccatg accacagact gcgactcctc ctcagaagga acagtcgccc ccgcccaatt
     8221 ggtctgcacc cagcgcgcca cccgcatcga aaccagcgcc gacaggaata accccacacc
     8281 gatcccgaac acgctgggac gctcgccgaa atcctgcgtc acccagccga aaatcgtcat
     8341 gccggcaatg gcggaggtca tcgcgccaag attataaatc gccagcaccc gccccagcag
     8401 cgatgccggg gcaatctcct gcaacacgcc ccaggccacc ggcgtgagcg tcccggcccc
     8461 catcccgatg atgaccatga gtgccgccgc gatcaaccga ttcgacgtca caattaaacc
     8521 gagcagcgcc aggccgctga tgaagctgga gagcgccatc aactggatcc gcttaggcaa
     8581 cgaccaggcc gacagggaca cgagccccag cgacaccagt aacaacccga tgccgaatgc
     8641 cgaccagagg tacccgacct cgatagggcc gaggtccagc aacttcttgc cgaacaccgg
     8701 aaagagcgtg ctgaaggcgc tcgtggcgaa ggtatacatg gacgccgctc cgatgagcat
     8761 caaaatgacc cgttggcgat ggagcacata gtggaagcca tcgagcacat cgctgaaggt
     8821 tcccgccagg gagccgccgc cgaccggctg agactcggcc cgtggaaagc gaatgaacgc
     8881 aaaacaggcg gcggaaatga catagctgac cgcattcaca cagagcactt cctgagaact
     8941 catggtcgcg atcccgacgc cactcagcgc cggcccgacg atgatcccga tgctggtcgt
     9001 cgtctgcagc agcgcattgg cggcggtgaa ttcgtggcgg gagaccagcg acggaatggc
     9061 ggcagtcaat gccggaccaa acaccgcgga ggcgaccgca tggacgaaca ccatgagata
     9121 cagccgctcg atactgaacg agtctaccgg caacaaacag ggcagcacgc ctaacaccag
     9181 cgcgcgaatc aggtcgctgc tgatcagcaa gagtttcttg ggaacgcgat cgacgatgac
     9241 gccgatgaaa ggaccgaaca gaatgggcgg cagggtctgc aacagcccga tcatggtggt
     9301 cttgagagga gaacctgtga tggagtagac gaaccagagc aacgcgagct tcgacacacc
     9361 atcgccgact tgcgagacca tctggcccca ccagacgagc gtgaagtcgc gcgtcagcag
     9421 atgcggcgac cacttcacgt tggggcgtcc cgttcctcct ccgttcacgt gccgtggctc
     9481 catggtgtca gcagactgcg tgtcggacct cctgtacccc tgtcgtcaca accgttccta
     9541 ttctccggcc accaaacgca cggcgtcggt attcacggcg cgcacgcccg gaacctgtcg
     9601 ggcggcttgc gccgcttcga gtacgatgat ctggaaccgc gtcgccccgt tcagggtgac
     9661 gatgccgtcc tcagttttca catcgaactt gaccgattgc aaggttttgc tcttctcgaa
     9721 ccgttctttc accaattgcg tgatgatttt gtcgcgttcc gcgaagagcc catgggccgc
     9781 gtcctgttca actttgagtc gattctcgac ggcgtgcacg ccgtcgagac accgcacgat
     9841 gtccgtcgcc acgcgcttat ccgattcctg cgacaccttt ccgagcagga cgagatcctt
     9901 gtccttggca tcgacctcga tgtcataagg aaacaggtgc ggatccgcca tgagcgccag
     9961 cttggccgtc accatcgacg aacgtatcgg tttcttacct tccggctccg ccgctttggg
    10021 gcgctctcct gcaccgggct ctcccggcgg tgcctgcgtc tctgccgccg gtttcacctc
    10081 tgccgcatgg ggcaccgttg gacagggatg ttcagccgcc gcatcaggct cttttgcttt
    10141 agactccggc tccttggact tcggctctac ctctttgacc ttgtgttcgg attccttggc
    10201 cttcgccgct ggctccttcg ctttcggctc ctgatccttg gccttggtct ccggttcctt
    10261 tactttcgac tccggctcct tgggtttcgc ctccgcctct ttgggctttt caggaggctt
    10321 ggcatctgat tccttgggtt tcgagtcagc ctccttcgtt ttcggctccg gctccttcgg
    10381 cttcgtatcg ggatccttcg gtcgttgcac cggcggggtt tcagactttc cctcaggcgt
    10441 cgattccgcc aaagcagttc cattcacatt tcccactccc gacgccgcta acagcgccag
    10501 gacacctgtc acgtagacgg ttcgaatcac ccgcataggc atgtgcccct catgtattga
    10561 atgaaaccga gactgcgaag ccgatcgccg agagcccgtc ttcaccatcg tctcggaaaa
    10621 cgaaacatcc ccggaagcaa agacatacgt gacggaagag cagaaagcaa caccgacaat
    10681 gccccgctag acagccggcg caagggcaag aaaaaacctg caagcgccgc ctggatgacc
    10741 tgcaggccta caaaaaccgg atgtaaaggg agagtattac gcaacagccg gactcaccac
    10801 gtggccacgg cggcttacct gcaccgtgac catcgcagtg agcaagagga gcagtccgat
    10861 cccgatgagg cttacagctg gacctagcgt atcagcggcc catccgaacc cagccatccc
    10921 gaccatggcg gaggccatcc cgccgacgct gaacgtggtg aacacacgtc ccagcaaatg
    10981 ttccggagtg acttcctgga gcatcgccca tacgaccggg tagaagagag acgtacttcc
    11041 gccgatgatg acgatcagca agaacgtgct gaacagcacc ggtgtctgaa ttaatcccaa
    11101 cgcacagacc gcaataccgc cgatcgccag agaacgaccg atcttgccga tgcgatcctg
    11161 gaaggtaccc tggggcgtcc gcgccagcca aatggaggct gccagcatcc cgatacccaa
    11221 ggccgaccac aaccagccca gttccatggg gccgacttgc aacaactcct tcgccaccac
    11281 cggaagaagg aatacgaagg cgctgatcgc caggttgtag agcacggcgg taatcatcaa
    11341 cgcgaatacc acccgatgct ggaggaacac gaagcggaat ccgaccatca tatcctgcat
    11401 gacgggtgtc gccaacacat cgagcccctt caccgtccgg ctgtcccgca cacgaatcgg
    11461 caggaggaag agcgcggaca ccagaaaggt cgcagcatcc acatagagca cattctgagc
    11521 gccgatcaac gcgatgccca atccgctcat ggccggtccc agcaagacgc cgatgttggt
    11581 ggtgctctgg agaaacgcat tcgccgtcgt cagttgcgac cgctgaacga tcagcgggac
    11641 cgcagacgcc agagcgggcc cgaacaccgt tgagacgatg gagatgagga agaccaacac
    11701 atacaaccgc tcaagcgtca gcatgtcgaa ggtatagaag agcggtatca ggagcaccat
    11761 caatgctcgc aagaaatcga ccacgatcat gaccgtcttc ttggggaggt agtccagata
    11821 gacgccgatc agcggaccga acagcagggg gggaatggtc tgtaacaacc cgatggcggt
    11881 catcttgagc gccgacccgg tcatttcata cacgaaccag agaagcgcca ccttgttcaa
    11941 accgtcaccg atctgagaga cgacctggcc ggcccagaga cagccgaaat cacgggtgcc
    12001 taataaccgc cacccgtcga cgttcgactc aggggtagat tgcgactcag ctgccatgct
    12061 tgtccttccg cgaggttaat gtcgcgctga ctagaggttc gaagcgtgct gctgcccgga
    12121 cttgccaggg agcgcagcgg catcagtgat tagctgctgg tagatcttta cgtagtcggt
    12181 cgtcatccgc tgggcagtga atcgctcgtc aaacatctgc cggcaccgat tgcgatcgat
    12241 cgtgccgagc ttgtcgacct gactcaccat ttcgtcgagg ttttcgctga tgaagccggt
    12301 gacaccgtga ccgataattt ccgggatgga tccccgtcgg taggcgagaa ccggtgtgcc
    12361 gcatgcaaga ctttcgatca acacgaggcc gaagggttcc ggccaatcgt aggggcagat
    12421 caggccgatg gcgttcccga taaaatcgct cttctccgca tcggtaatct ctcccacaaa
    12481 ctcgatcaag ggatggtcga ggagaggctc gacgacccgc tcgaaatagg cccggtccgc
    12541 cgggtcgacc ttcgccgcca tcttcatagg tattccgaca cgtttagcca gttcgatcgc
    12601 ttgatccgga catttttccg gcgagacccg ccccaggaac gccaagtact tacccggttc
    12661 aggatggaat ttatagaggt cgtgcggcag gccgtgatag acggtgtttt gccaattgca
    12721 ccaaggcaac ggccgacgct gcgaatcaga aatggagacc agcggcagct ccgcaaagtc
    12781 acggaagacc ggcacgagtt cgggcaaatc caaccggcca tggagcgtcg tcacgacggg
    12841 cacccgacat cggcgagaga gagaaaaggc cagaaagtcg agatgtgagt gaatcagatc
    12901 gaactggtcg gcggaagcga agacctgttc catcatctga atgagcgggg cttcgcggtt
    12961 gaagatcccg gtgttcaaac gcagggcctg ctggcaaggg gcctccagct ttgcccgcgt
    13021 gaccgaatca ccgctagcga acagcgtcac ctggtggcct tgacggacga gttcttccgt
    13081 caggtaggag acgatgcgtt ccgtgcctcc gtacaacttt ggagggacgc tctcccacag
    13141 cggcgatacc tgggcaatcc tcataaggac aatctccttt agtcttgaac ggtcagcggc
    13201 cggtgccgga aacggcgtcg aatattacac cacgcccacc accatctgcc gcattctttc
    13261 tgttctgcct atccgattca attgacatct ttgacacggg ctgattgttt ttgtcgtctt
    13321 gatgccgatg gagtgaatgc tcgcgatgac cggagggcaa agcaagcgtt gtaccgccac
    13381 gtgcctcagg agtgaggctc acgcaaacaa gcattcaaaa caattattaa tcaataactt
    13441 agcataacca cctcaacatt tcatctggac atcgatggat ttgtgggctg tggattctgt
    13501 tgataattca gctcccggcc cgaatcggct gtgacaggct tgcgtcgaca aaccaaaccg
    13561 ccacagagat ttcctgcttc gcgctcagaa aatgtcctcc gtccgattcc cgctacttcg
    13621 acgaagcctt gcgcacgcat ccattacgac agacgcgggg cgccacaaag aagaaaacct
    13681 tcgttcaggg aaatattgag tatcgaaagg cgagaccatc cgccgcaggg gatgatccgc
    13741 caacgcggcc gagcgggtga tccaacatac tgcttaaccc ggactattca tgaatgcggt
    13801 cgcgaggcgt tcgagacgga agcccgccga ggctgctcgc acgtcaggcc gacgcaggcg
    13861 tacgtgcagt ccgtcgagga ggccgaacga gaacagcctc gccgggcgac aggatcggac
    13921 gccgaagcag gtatttatga atagtccgga ctagggaatg aggtcgtcga gcagacccga
    13981 ctgaacggga gaaagcggcc ccgcctcacg cgggacggga ggaaagacca
//
LOCUS       DAOQRM010000060        13526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3618_length_13526_cov_17.738253, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000060 DAOQRM010000000
VERSION     DAOQRM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13526
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3618_length_13526_cov_17.738253"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="PKK81_06625"
     CDS             complement(<1..1338)
                     /locus_tag="PKK81_06625"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNV32583.1"
                     /translation="MQIATRLFVRNVWLQAVVMALASLALSEAMWAPAPVTYHALEWA
                     PYDTWMRLRSQPAPDSHLLLVMRDQASEQQFGAGLWDRSLPARLITGLHDAGAAAIGL
                     DIPLDFPSPPNLGGAVSDALLMEAVTSAGTVSYPAFPTAPIDQDRVGFAVPAQGLTPG
                     RSTLQPLLDLDRIARRAGLFYGNGSDEFPALGFSLATTFWQIPLRQVERRSGQVRVQN
                     ARWPGGTTGSLTIPLDRQGRLLLNFAGRQVPTAFPGTTVLELSRLLDRKDNEALGTLV
                     NGKVVVLLIQPHSQPERTLPSGDDASDMLLQAHLLHTLLTQDWIHELSPLQRLLVTFA
                     LCLWVAWLVLRWTDWKGLLLGAGSLLLYLTFGLVSLTTAHWVLPFVIPVTAAVTVLVA
                     TSLLGQVVATRRLALLEQDMLQVQQNLGAVREALIFRETAVESLEEDLESARAG"
     gene            complement(1517..2791)
                     /locus_tag="PKK81_06630"
     CDS             complement(1517..2791)
                     /locus_tag="PKK81_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32584.1"
                     /translation="MFLHHATKGGWPLILAMALLIHPAWARAQEDPSAIPAPEPIREE
                     PLPPESVAQPALPTPIPAPVPLSLLETLTQARQALHLEPDAQEPRLTLGRTLFQLGDT
                     DGAIDEYRTALRFHPTVAQAHLDLGTALMAKQDWRSAMTELQEAVRLDPTLVQAHYSM
                     GTIHYTRGNVQSAIKAYQEALRLKADFAEAHYRLGLVLKVAGRDKDAAQELEAAALAG
                     LAKAQYFLGNAYRSGQGVEKSQIMAITWWARAFEQGLPEAAQALTQLRRLAAVKGNLQ
                     TKQSKAAAEAFKNYCDQLWLDFPDLDRGDQATETVGTTLLKQGRTGEALPVLLREAYA
                     LNDISHAALVQLYEQGLDGQLPPHGQWILSYLESTAADGAIPSRTALARIYAKGLGLA
                     PDLAKAKSYLKGMPREEVKRILDEITPETPKP"
     gene            complement(2905..3567)
                     /locus_tag="PKK81_06635"
     CDS             complement(2905..3567)
                     /locus_tag="PKK81_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32585.1"
                     /translation="MANQPPPPSANRPRTFLRALLWSLPLHVLVSIAVVGGAAAYYSN
                     TVSSHGTWAAVGSWSLTALYVVGALVGGALAGVLSAAQRTVERLELTLRDWLHALPAM
                     QSASTSVGRSLATARQEYETMVDRCVTQTAGRLRLPRWLERLIRAALQGVVVDRFMTS
                     CTDRGLNLVAPQEFRNWLLAEGVSLGFMPIQDQLSWWRSLILGLLGLLVAIVLALAFL
                     NN"
     gene            complement(3586..4035)
                     /locus_tag="PKK81_06640"
     CDS             complement(3586..4035)
                     /locus_tag="PKK81_06640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNV32586.1"
                     /translation="MSSQSCKACVGTWPDRSHFIADCGLTRAYLHDDQFFPGWTVLVL
                     KRHATELFHLSRDERSRLMEEVSGVAALLAQEWQAVKVNYELLGNQLPHIHWHLIPRL
                     PRDPAPLEPVWRIAHEPVRLEPDALASEIDRLRKIWPFHPNQSPVNP"
     gene            complement(4032..4655)
                     /locus_tag="PKK81_06645"
     CDS             complement(4032..4655)
                     /locus_tag="PKK81_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32587.1"
                     /translation="MAKTRSMHIVVGSACLLLAQAFTPLLSAAAELIDLSGKAAAFVT
                     LRSRDNFSSEYRYDVTVRNNSADLFIADSLIIVLDRITNIGGQDRENLTGESLLNRME
                     IIGQDGTTEDGRPFFRVPQGSGPDLTPNSESLPVTVRIRNRDYVAVFTPSFRVMGQKR
                     DAPKQKSPAAAAAAAAAPNKATTDKLIQLLIKKGLITPQEAQSLSQP"
     gene            4814..>5138
                     /locus_tag="PKK81_06650"
     CDS             4814..>5138
                     /locus_tag="PKK81_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32588.1"
                     /translation="MATTRMTSPPLLVPRTLVSQLLHLYDYPDPRRPGRLIKGYDCPH
                     ALRTARMCAAVAQRMGHQPARIKQYQIACILHDLGRAGLDRRLFGRIWSWARAQGIPT
                     RPREWR"
     assembly_gap    5139..5238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5239..5436
                     /locus_tag="PKK81_06655"
     CDS             <5239..5436
                     /locus_tag="PKK81_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HNV32589.1"
                     /translation="GWQHNVRNAGEDNGHSHLRGQLQGQSLTIPFNDGVMTLGTWQQI
                     VVLDFDTRARTRDLVLQVIGE"
     gene            5438..6559
                     /locus_tag="PKK81_06660"
     CDS             5438..6559
                     /locus_tag="PKK81_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNV32590.1"
                     /translation="MRLSGAIRKRWRGKRLRVGLVAALALSSPAWLQAAEGTNGLLIA
                     QSAAPTELSPEEQATIAVFERATRSVVFIANTAMQRDPWSFNLFEVPQGSGTGFVWSR
                     QGHIVTNYHVIYGADAITVTLADRTEYKAKVVGADPDHDLAVLQIQAPETALQPVIIG
                     NSQSLRVGQKVLAIGNPFGLDHTLTTGVVSALGRTIKSMSNRTIEGVIQTDAAINPGN
                     SGGPLLDSGGRLIGVNTQIMSPSGAFAGIGFAVPVDTVSRIVPELIKHGKLIRPGLGI
                     SLVPDAMARRWGVKGVIIGKVGRGSIAERIGLHGARETVGGRIELGDIIVAVDGQPVE
                     TIDDLMDLMEKHKVGDEVTIDYVRGKRRLQVMAPLQAVN"
     gene            6657..8408
                     /locus_tag="PKK81_06665"
     CDS             6657..8408
                     /locus_tag="PKK81_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV32591.1"
                     /translation="MSDHRNPDQPESGGQQPPRREASASSDPIELIEECLAAFPDGDP
                     RQKLLYKLRHVITAQSVAQDRRDTELKKLNEVVAKLTAPANRVGLLLEVPAEAVARIV
                     VGGAEYYANIDPRLPVEDLKIGTQILVNEAYTVIKALGYDRNGPVLKVAEVLPDGRIR
                     FEQDMGRQALILQRSSDLLGADLKAGDEVRIEPTHRIAIEKFENRQARTHLLDEVPSV
                     TWAQIGGQHQAIEAIRKAIEYPLLHADTFTKYQFTQPKGFLLYGPPGCGKTLIGQAAA
                     ASLAQLVRESQGQAAADGTSKNPPVTSGAFLHIKGPEILNMWLGESERIVRDLFAKAR
                     ARRKEGALPFIFIDEAESVLGTRRSMRSFNINNTLVPMFCAEMDGIESLHDVVIILAS
                     NRPDLIDPAVLRPGRIDRKIKVARPSREAAVEILAVYLTPSLPLDCELLEQNGQDHEA
                     ARRAVIEQVVDSLFTRTDQNRVLSIRLRNGQNKVLYRGDLVSGAILSSIVQRAKEKAI
                     ERAVAEAGAPAGDGIRAQDLLDAVHEEYREGEMLPPDDAAEEWLKLLDHHPEQVVGVS
                     SFRRGRPTEERLVNQII"
     gene            8417..9937
                     /locus_tag="PKK81_06670"
     CDS             8417..9937
                     /locus_tag="PKK81_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HNV32592.1"
                     /translation="MHLFGIETEYGITREDLDAVDPVEESMELVRAHLPGKFERRWDY
                     RGEDPHEDARGFRVSGLQQDKEEDDFAKVDAHRPFSFHEMKSDLVLPNGARFYNDHTH
                     PEYSTPECRTLKDLLAHDRAGERIVQRAADRRNQQLGGAHVQLYKNNTDFHGHSYGCH
                     DNYLVSRSLPFAQLVSGLLPFLVSRQLVAGAGKVGMEAQESGFVPGPFQISQRADFME
                     AELGVDTMHNRPILNTRDEPHADRTKYRRLHLILGDANMCEYATALKVGTTRLVLDLL
                     QRGEAPSLELEQPVAAIKQLSRDPDLKTTVRLKDGRTISGLAIQEEYWNVASRCCAGS
                     DPDADWVLREWQETLCALRQDRTQLVGKIDWVTKQWLLETFMREERLAWEDPWLASLD
                     LEYHNVNPDRGLFLGLEAEGKTWRMTSERDIAQALVAGPSDTRGGLRGLCVRRFPEQI
                     TGMQWERVQFSGGLRARTLEMGDLFEPDAVQACAALLEAAASPADALAAWSRRKDG"
     gene            9940..10134
                     /locus_tag="PKK81_06675"
     CDS             9940..10134
                     /locus_tag="PKK81_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HNV32593.1"
                     /translation="MRYMLMPERREAPGDPMPKPSGPSEEGGGPRRPETGSPDKDNLL
                     KRMRKVDPKQAERYRQRTGQ"
     gene            10239..11012
                     /locus_tag="PKK81_06680"
     CDS             10239..11012
                     /locus_tag="PKK81_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HNV32594.1"
                     /translation="MGMQGDFFQLLKEQGYQFGNPVAAAAGMDVPTATTILALKYRDG
                     VLVAGDRRATAGNIVMYDRTDKVLEIDRHSVMAIAGVPATAYEMVRVLEHSFKYYRRT
                     QLQELSFEGKLRAVSKLLKENVPAALAGTGAVVPVFAGYDHEQESAKIYFYDILGAEF
                     EAVEYAVSGSGSPTIRGILHYVNTWGPQPLATLPEEQATIQALRLLSSAAEFDSATGG
                     VNRELNLYPVVKLIKASGVQTIPDADLKRLHESEVLRGR"
     gene            11059..11898
                     /locus_tag="PKK81_06685"
     CDS             11059..11898
                     /locus_tag="PKK81_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32595.1"
                     /translation="MYEEPYRWIEAVGNRRQYLDEQFAKGSPVVALAYADGILMLTVS
                     RGTPKLYEIYDRIALGGMGHPADLEKLRFSLLEMAHVEGFNRSPSDVTGARLMKYGLA
                     PTIKQAFEEIYKAPFIAKILLAELGVKPERNLFLSINYDGNFEENQGWAALGATASVQ
                     GRMRRYVESQPAFAQASLAQAVELALCTWAVGSLAQAASESPAAESSSTQLSEEQTEG
                     DGSSLPDKAALLAHVRQTVAEKSLECVLLERRGPGSAKYRALRPEELGRLLPSAVSSP
                     ATT"
     gene            11900..13390
                     /locus_tag="PKK81_06690"
     CDS             11900..13390
                     /locus_tag="PKK81_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HNV32596.1"
                     /translation="MLNRIFGLETEYGLLVNQDRPDHSPSWVAQRIRDHIFHVERRGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYIDMGHLEYASPECTTLADLVASDRAGDRIIQDA
                     VRALGLDETVSLIKNNIDHETDATFGSHENYLVTRQFPFSRRGLGPLVTFLVTRQVFT
                     GAGRIGCASDPNEWVQVGGLILHRPGLRDAQDRSIVPFQISQRADYIVNDFFEWVQHN
                     RAIVNTRDEPLADPNQYRRIHLLCGDSNMAEYATALKMGTTGLVLQLIEAGQAPRGLG
                     IHEPVEALQDISRDPDRRWIVRLESGQSMSAIDIQEQFLAAAQRHCKGQDEETDWVLE
                     QWESVLTDLRGGYEKLVGRIDWASKLWLLETYREAEQCAWDDPMLKSLDLEYHNLHPE
                     RGLCYGLEEEGRGPRLTTDKIVHLAQDHPPRNTRAFGRGELVRHLLASGGGGIVSDAP
                     LDFEEQMACEYIINWSNFKLRGTAPFFMADPFKTYVQDVRSHLAAR"
BASE COUNT         2632 a         3727 c         4451 g         2616 t
ORIGIN      
        1 gccggcgcga gccgattcga ggtcttcctc cagggattcc actgcggtct ccctgaagat
       61 cagtgcttcg cgaaccgccc ccaggttttg ctgaacctgc agcatgtcct gctccagcaa
      121 ggccaggcgt cgggttgcca cgacctgccc tagcaggctg gtcgccacga gcacggtgac
      181 ggctgccgtg acaggaatca caaacggcag aacccaatgg gccgtggtga gcgagaccag
      241 cccgaaagtc agatacagca ggaggcttcc ggctcccagg agaagcccct tccaatccgt
      301 ccaccggagg actaaccagg cgacccagag acagagggca aaggtgacca gtagcctctg
      361 aagtggggag agttcgtgaa tccagtcttg cgtgagcagc gtgtggagca ggtgtgcctg
      421 gaggagcata tccgaggcgt cgtcgcctga agggagcgtg cgttccggtt gcgagtgcgg
      481 ttggatgagc aggacaacga ctttgccgtt gaccagcgtg ccgagcgctt cattatcctt
      541 ccggtcgagg aggcgggaaa gttccaacac ggtggtgccg gggaaagccg tgggaacttg
      601 tcgtccggcg aagttgagca ggagtcggcc ctgacgatcg agcgggatcg tcagggagcc
      661 tgtcgtaccg ccaggccacc gtgcgttctg cactcgaacc tgtccggagc gtcgctccac
      721 ctggcgcaat ggaatctgcc agaacgtggt ggctaaggag aagcccaggg ctggaaactc
      781 gtcggatccg tttccataaa agagcccggc ccggcgcgca atgcgatcga ggtccaggag
      841 cggctgtagg gtgcttcggc ccggcgtgag gccctgtgcg ggcacagcaa agcctaccct
      901 atcctgatcg atgggcgcag ttggaaatgc ggggtaggag acggttccgg cggatgtcac
      961 tgcctccatc agcaaggcgt cgctgacggc gccgccgagg ttgggagggc tggggaagtc
     1021 gaggggaata tcgagcccga tagccgctgc tcccgcatcg tgcaatccag tgatgagcct
     1081 ggccgggagg ctgcgatccc aaaggccggc gccgaactgt tgttcgctgg cctgatctct
     1141 catgaccagg agcaggtgtg agtcgggggc cggttgcgag cggagtcgca tccaggtgtc
     1201 gtagggtgcc cattcgagcg cgtggtaggt cacaggcgcc ggcgcccaca tggcctcgga
     1261 gagggcgagg gacgccaatg ccatgacgac ggcttgcagc cagacattcc tgacgaacag
     1321 gcgagtggct atttgcatgg gtcgtggcga ctagcgggat gatgaacagg ttcgtcggtg
     1381 cccgcatcca tcgcccgcat gggggagtgg gttgggtcga gcggactgtg cgaacatcct
     1441 gcgagcggtt ctgctcctgc ccgtgagaca taccgtccgg catgtcgagg ccattgaaat
     1501 catgtccaac agcttactac ggttttggag tctcaggggt gatttcgtcg agaatacgtt
     1561 tgacctcttc ccgcggcatc cccttcagat aacttttggc cttggcgagg tccggtgcca
     1621 ggccgagccc tttggcgtag atgcgagcca gggccgtgcg tgaaggaatc gcgccatcgg
     1681 ctgcggtgga ttccagataa ctcagaatcc attggccatg ggggggaagc tggccgtcga
     1741 ggccttgttc gtagagctgg accagcgccg catgcgagat gtcgttgagc gcataggctt
     1801 cccgtagcag caccggcagg gcctcgcctg tgcgcccttg tttgaggagt gtggtgccga
     1861 cggtttccgt ggcctggtcc ccccgatcca gatcggggaa atccagccag agctgatcgc
     1921 aataattttt gaaggcctcc gccgccgcct tggattgttt tgtctgcaga ttgcctttga
     1981 ccgctgccag ccgccggagt tgggtcagcg cctgagccgc ttccggcagg ccctgctcaa
     2041 aggcccgcgc ccaccaggtg atggccatga tctggctctt ctctacgccc tggcctgagc
     2101 gataggcgtt gccgagaaaa tattgagctt tggccaatcc cgccagcgca gcggcttcca
     2161 gttcctgcgc cgcatccttg tcccgtcccg cgactttcaa gaccagcccg aggcgatagt
     2221 gggcttcggc aaagtccgct ttaagccgca gggcttcctg ataggccttg atggccgatt
     2281 gtacatttcc gcgggtgtaa tgaatcgttc ccatgctgta gtgcgcctgc acgagggtcg
     2341 gatccaagcg gacggcttcc tggagctccg tcatggcgct gcgccagtct tgcttggcca
     2401 tgagcgcggt gcccaaatcg agatgggcct gcgcaacggt cggatggaat cgtagggcgg
     2461 tccggtattc gtcgatggcg ccatcggtat cgcccagctg gaagagggtt cggcccagcg
     2521 tgaggcgtgg ctcctgggca tcgggttcga ggtgtaaggc ttggcgagcc tgggtcaggg
     2581 tctccaacag gctgaggggc accggcgccg gaatcggcgt cggcaatgcc ggctgagcga
     2641 cggattcagg ggggagcggc tcttctcgaa tcggttccgg ggccgggatc gcggaggggt
     2701 cttcttgcgc gcgcgcccaa gccgggtgaa tgagcaacgc catggccagg atcaaaggcc
     2761 accctccctt ggtggcgtgg tgcagaaaca taagtgctgg caatcctcca catgaaaaaa
     2821 gacgttcctt tatacacata gacgaagcgg aaattccacg ggatctcgca tgaaacgtgt
     2881 gccgggatga tgtgcgggga gcattcaatt gttgaggaat gcgagcgcga gaacaatcgc
     2941 gaccagcaga cccaggaggc ccagtatgag ggagcgccac caggacaatt gatcctgaat
     3001 cggcatgaac cccaggctga cgccttccgc cagcaaccag tttctgaatt cctgcggggc
     3061 gacgaggttg agtccccggt ccgtgcagga ggtcatgaac cgatccacaa ccacgccctg
     3121 cagcgccgcg cgaatcagcc tttccagcca acgcggaagt ctcagacggc cggcggtctg
     3181 agtcacgcat cggtcgacca tggtctcgta ttcctgtcga gccgtggcaa gacttcgtcc
     3241 taccgaagtt gacgccgact gcatggcagg caaggcatgg agccagtccc ggagcgtgag
     3301 ctccagccgc tccaccgttc gttgggctgc gctcaacact cccgcaaggg cgccgccgac
     3361 cagcgcaccg acgacgtaga gggccgtcag cgaccagctg cccactgcgg cccatgtgcc
     3421 atgggaggat actgtgttgc tgtaataggc ggcagcccct ccgacgacgg cgatcgagac
     3481 gagcacgtgc agcggcagag accagagcag agcccggagg aaggtgcgag gcctgttcgc
     3541 gcttggtggg ggaggctgat tcgccatcgt ggccagtcga cgatctcagg ggtttacggg
     3601 ggactggttt ggatgaaacg gccagatctt cctaagacgg tcgatttccg aagcgagtgc
     3661 gtccggttcg agtcggaccg gttcatgcgc aatacgccac accggttcga gtggcgcggg
     3721 atcccgagga aggcgtggaa tcagatgcca atggatgtga ggaagctggt tgccgagcaa
     3781 ctcatagttg accttcacgg cctgccactc ttgcgcgagc agggctgcga ccccggacac
     3841 ttcttccatg aggcggctgc gttcgtcgcg agagaggtga aagagttccg tggcgtggcg
     3901 tttcaggacg agcacggtcc agccggggaa gaactggtcg tcatgcaggt aggcccgggt
     3961 gagtccgcaa tcggcgatga aatgactccg gtcgggccag gtgccgacac aggccttgca
     4021 cgactggctg ctcatggttg gctcagagac tgggcttcct gcggcgtgat caggcccttc
     4081 ttgatcagga gttgaatcag cttgtccgtg gtggccttgt tcggtgcggc cgcggcggca
     4141 gcggccgccg ccgggctttt ctgtttaggt gcgtcccgtt tttgccccat cacccggaaa
     4201 gacggggtaa agaccgcgac atagtcccgg ttcctgatgc ggacggtgac ggggaggctc
     4261 tcgctgtttg gggtgaggtc cggccccgat ccttgaggga cacgaaaaaa aggtcttccg
     4321 tcctcagtgg tgccgtcctg gccgatgatt tccattctgt tcaggaggga ttcgcctgtc
     4381 aggttctcac gatcctgccc gccgatattg gttatgcgat ccaggacgat gatcaaggag
     4441 tcggcaatga agagatcggc agaattgttg cggaccgtca catcgtaccg gtattcgctc
     4501 gaaaaattat cacggctacg cagggtgacg aatgcggcgg ctttgccgct caaatcaatc
     4561 agttcggctg cagcggagag caggggcgtg aaggcttgag caagtaggag gcaggcgctt
     4621 cccacaacaa tgtgcatgga ccgcgtcttc gccatgatcc cctcccggtt gtcggtcagc
     4681 atcagcctca gaataacaaa gccccacagt gagtgcgagg aaatcctctg cccgggtcgt
     4741 ggtttgcttg acacgttctt ttcacggacg atattctgcc gttcgatcat cgactgcgac
     4801 tcatcacacc tgcatggcaa ctacacgtat gacgtcccct ccgctgcttg ttccgcggac
     4861 actcgtttcc caactgctgc atctctacga ctatcccgac ccgcgccgtc caggacggct
     4921 gattaagggg tacgactgtc cgcatgccct ccggacggcc cggatgtgcg ccgctgtggc
     4981 tcagcgcatg ggacaccagc cggcacgaat caaacagtat cagatcgcct gcattctgca
     5041 tgatttaggt cgagccggac tggatcgacg gctcttcggc cggatctggt cctgggcccg
     5101 cgcgcaggga attcccacca ggccgcgtga atggcgtgnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnngg ctggcagcac aatgtgcgca acgcaggaga ggacaacggc
     5281 cacagccact tgcgcggtca attgcagggg caatcgctca ccattccttt caacgacggg
     5341 gtgatgacgc tgggtacctg gcagcagatc gtcgtgttgg atttcgatac gcgcgcacga
     5401 accagggacc tggtgttgca ggtcatcggt gaataggatg cgtctgagcg gggcgatccg
     5461 gaagaggtgg cgcgggaaac ggttgagagt tggtttggtg gcggcgctgg cgctgtcgtc
     5521 accggcctgg cttcaggccg ccgagggcac gaacgggctt ctcatcgcgc aatctgccgc
     5581 gccgactgag ctcagtccgg aggagcaggc gaccatcgcc gtgtttgaac gcgctacgcg
     5641 gtcggtggtg ttcattgcga acacagcgat gcagcgcgat ccctggtcgt tcaacctctt
     5701 cgaagtgccg caaggctcgg ggacgggctt cgtgtggagc cggcagggcc acatcgtcac
     5761 caactatcat gtcatttacg gcgcggatgc gatcacggtg accttggccg atcgcaccga
     5821 atacaaggcg aaagtcgtcg gcgccgatcc ggatcacgat cttgcggtct tgcagattca
     5881 ggctccagaa accgcgctcc agccggtgat catcggcaat tcccagtcgc tgcgtgtcgg
     5941 gcagaaggtg ctggccatcg gcaatccctt cgggctcgac cataccctga ccacgggagt
     6001 ggtgagtgct ctcggccgca cgatcaagtc gatgagcaac cggacgattg aaggcgtgat
     6061 ccaaaccgat gcggcgatca acccgggcaa ttccgggggg ccgctgctgg acagcggcgg
     6121 ccggttgatc ggcgtgaata cccagatcat gagcccgagc ggagcgttcg ccggcatcgg
     6181 gttcgcggtg cccgtcgata ccgtcagccg gatcgtgcct gaattgatca aacatggaaa
     6241 gctgatccgt cccggactgg gcatctcact agtgccggat gccatggccc ggcgttgggg
     6301 tgtcaaaggt gtcatcatcg gaaaggtcgg ccgggggagc atcgccgaac ggatcgggct
     6361 gcatggggcg cgtgagaccg tcggcggccg gatcgaactg ggcgatatta tcgtggcggt
     6421 ggacggccag ccggtcgaaa ccatcgacga tttgatggat ctgatggaga agcacaaagt
     6481 cggcgatgag gtgacgatcg actatgtccg gggcaaacgt cggctccaag tgatggcgcc
     6541 gctgcaggcg gtcaattgaa ttgatcgggc gggtgtgaac cggcccgtgc gaccgcgaca
     6601 gtggagttgt gctctgtgtt aggatgaccg gagttggacc agccggagga attgatatga
     6661 gcgaccatcg aaatccggat cagccagagt cgggcgggca acagccgccg cgccgtgagg
     6721 cctctgcctc gtctgatccg atcgaactga tagaagaatg tctggcggcg tttccagacg
     6781 gcgaccctcg ccagaagctg ctctataaat tacgacatgt gattacggcc cagtcggttg
     6841 cccaggaccg gcgcgacacc gagttgaaga aactcaatga ggtggtcgcc aagttgacgg
     6901 cgcctgctaa ccgagtcggc ctgctgctcg aagtgccggc ggaagctgtg gcgcgcattg
     6961 tcgtcggagg ggcggagtac tacgccaaca tcgacccgcg tctcccggtc gaggatctta
     7021 agatcggcac gcagattctg gtgaatgaag cctatacggt gatcaaggcc ctgggatatg
     7081 accgcaacgg gccggtgctg aaggtggccg aggtgttgcc ggatgggcgc attcgtttcg
     7141 agcaggatat gggccggcag gcgttgattc tgcagcggtc cagcgacttg ctgggggccg
     7201 atctcaaagc cggcgacgaa gtccgtatcg agccgacaca ccggatcgcc attgaaaaat
     7261 tcgagaatcg tcaggccagg acgcatcttc tcgatgaagt ccccagtgtg acgtgggcgc
     7321 agatcggcgg ccagcatcag gcgatcgaag ccattcgaaa agccatcgaa tatcctctgc
     7381 tccatgccga cacgttcacg aaatatcaat tcacgcagcc gaagggattc ctgctctatg
     7441 gaccgccggg ctgcggcaag accttgatcg gacaggccgc agcggccagt ctggctcagc
     7501 tcgtccgtga atcgcagggg caggctgccg cggatggcac gtcgaagaac ccgccggtga
     7561 caagcggcgc cttcctgcac atcaaggggc cggagattct gaacatgtgg ctgggcgaat
     7621 ccgagcggat tgtccgggac ctttttgcca aggcgcgcgc cagacggaag gagggggcgc
     7681 tgccgtttat tttcatcgat gaagccgagt cggtgttggg cacgaggcgg tcgatgcggt
     7741 cgttcaacat caacaatacg ctggtgccga tgttctgcgc tgagatggat ggcattgagt
     7801 cgctgcatga tgtggtgatc attctggcct ccaaccggcc ggatttgatc gatccggcgg
     7861 tcctcagacc cggtcgaatc gatcggaaga tcaaggtcgc gcggccgagt cgggaggcgg
     7921 ccgtggagat tcttgcggtg tatctcacgc cgtcgttgcc gctcgattgt gagttgctgg
     7981 agcagaacgg gcaggaccat gaagctgccc gtcgcgccgt gattgagcag gtcgtcgaca
     8041 gtctgtttac acgaaccgat cagaatcgcg tgttatccat tcgccttcgg aacggtcaga
     8101 acaaggtgct gtatcgcggc gatcttgtca gcggcgcgat tctctcttcg atcgtccagc
     8161 gggcgaagga gaaggcgatt gagcgggcgg tggcagaggc cggggcgcct gccggcgacg
     8221 ggatccgcgc gcaagatctg ctcgatgcgg tgcatgagga atatcgcgaa ggtgagatgt
     8281 tgccgccgga cgatgccgcc gaagaatggc tcaaactgct ggatcaccat cctgaacagg
     8341 ttgtcggtgt gtcgtccttc cgccgcgggc ggccgacgga agagcgattg gtcaaccaga
     8401 ttatctgaca cggcctatgc atctcttcgg cattgaaacg gagtatggca tcacccgcga
     8461 ggatctggat gccgtcgacc ccgtcgagga atcgatggaa ctggtccgtg cccacttgcc
     8521 cggcaagttt gaacggcgct gggactatcg aggtgaggac ccgcatgagg acgcgcgcgg
     8581 tttccgagtc tccggtctgc aacaggacaa ggaagaggac gacttcgcca aggtcgatgc
     8641 gcatcgacca ttttcgtttc acgagatgaa gagcgatctg gtcctgccga acggtgcgcg
     8701 gttttacaac gaccatacgc atcccgaata ttccactccg gaatgtcgca cgctcaaaga
     8761 tttgctggcg catgatcggg ccggagagcg cattgtgcag cgggccgcgg atcgacggaa
     8821 tcagcaactc ggcggtgcgc acgtccaact gtataagaac aataccgact ttcacgggca
     8881 cagttacggc tgccatgaca attacctggt gtcgcgctcc ctgccgtttg ctcagctggt
     8941 cagcggtctc ttgccgtttt tggtcagccg gcagctggtg gccggagccg ggaaggtggg
     9001 gatggaggca caagagtccg ggtttgtgcc cggtcccttc cagatttcgc agcgggccga
     9061 ctttatggag gctgaactag gcgtcgatac gatgcacaac cggcccatcc tcaatacccg
     9121 tgacgagccg catgccgacc ggactaagta ccgacgtctg cacctgatcc tcggcgacgc
     9181 gaacatgtgc gaatacgcga cggcgctcaa ggtcggcacc accaggctgg tgctggacct
     9241 gctccaacgt ggagaggccc caagcctgga attggaacag ccggtggctg cgatcaagca
     9301 gctctccaga gatcctgatt tgaagacaac ggtgcgattg aaggatggcc ggacgatatc
     9361 gggactggcg atccaagagg aatattggaa cgtggcgagt cgatgctgtg cgggcagtga
     9421 tccggacgcc gactgggtgt tgcgggaatg gcaggagact ctctgtgcgc ttcgtcagga
     9481 tcgcacgcaa ttagtcggga agatcgactg ggtgaccaag cagtggctgc ttgagacatt
     9541 catgcgggaa gagcggctgg cgtgggaaga tccatggctc gcgagtttgg acttggaata
     9601 tcacaatgtg aaccccgacc gaggcctgtt tctgggcctc gaagcggaag ggaaaacctg
     9661 gcgcatgacg agcgagcgtg acattgcgca ggccctggtg gccggcccct ccgataccag
     9721 gggcgggctt cgcggattgt gtgtgagacg gttcccggag cagatcacgg ggatgcaatg
     9781 ggagcgggtg cagttttccg gggggctgcg tgcgcggacg ctagagatgg gtgacctctt
     9841 tgagccggat gcggtgcagg cctgtgccgc gttgttggaa gctgcggcgt ctccagccga
     9901 tgcgctggcg gcgtggtcga gacgaaagga cgggtaacca tgcgatatat gttgatgccg
     9961 gaacgtcgtg aggcgccggg cgatcccatg ccgaaaccgt cggggccgtc tgaagaaggc
    10021 ggagggcctc ggcggcctga aacgggctcg ccggataagg acaatctgct gaagcggatg
    10081 cggaaagtcg atccgaagca agcggagcgt tatcggcaac gcacgggaca ataaacgtga
    10141 aaggtaaaaa gtgaaacgtg aaaggtcgcg gggctgagga ttgaagcgat cgttggtcca
    10201 cctttggcgt ttacgtttca cgtctaacga ggtaagcaat ggggatgcaa ggggattttt
    10261 ttcagctctt gaaggaacag ggatatcagt tcgggaatcc tgtcgcagcg gcggccggga
    10321 tggacgttcc gacggccacc acgattctgg cattgaagta tcgcgacggc gtgttggtcg
    10381 caggcgatcg tcgggcgacg gcgggcaaca tagtgatgta cgaccgcacc gacaaggtgc
    10441 tggagatcga tcgccacagt gtgatggcca tcgcaggggt gccggcgacg gcgtatgaga
    10501 tggtacgggt gctggagcat tcattcaaat actaccggcg cacgcaattg caggagttga
    10561 gtttcgaagg aaaactgcgg gcggtctcca agttgctcaa ggagaacgtg ccggcagcgt
    10621 tggcagggac cggtgccgtg gttccggtct ttgccggata tgaccatgag caggagtctg
    10681 ccaagattta tttttacgat attctgggtg cagagtttga agccgtggag tatgccgtgt
    10741 ccggttctgg ctcaccgacg attcgaggca tcctacatta tgtgaacaca tggggcccgc
    10801 aaccattggc aaccctcccg gaggaacagg ccacgatcca ggcgctccgg ttgctctcca
    10861 gcgcggcgga attcgattcg gccaccggcg gggtgaatcg ggagctgaat ctctatccgg
    10921 tggtgaaact cattaaggcg tccggtgtgc agaccattcc ggatgccgac ttgaaacgat
    10981 tgcatgagag cgaagtgctg cgcggacgct aacagataga cggctggtta ttgaccccta
    11041 tggtgaaggt ggcacgttat gtatgaagaa ccctatcggt ggatcgaggc ggtcggcaat
    11101 cgacgccagt atctcgatga acaatttgca aagggctcac cggtcgtcgc gttggcctat
    11161 gccgacggga tattgatgct gaccgtcagc cgcggaacgc ccaagctgta tgagatttac
    11221 gatcgcattg cgctgggtgg aatgggacac ccggcggatc tggagaagct gcgcttctcc
    11281 ctcctggaga tggcccatgt cgaggggttc aatcgatcgc cgtccgatgt cacgggcgcc
    11341 aggctcatga agtatgggct ggcaccgacc atcaagcagg cctttgaaga aatttataaa
    11401 gcccccttca tcgccaaaat tttgctcgcc gaattgggcg tgaaacccga gcgaaacctg
    11461 tttctgtcca tcaactacga cggaaatttc gaggagaacc agggctgggc ggcattgggc
    11521 gcgacggctt ccgtgcaggg ccggatgcga cgctatgttg aatcgcaacc ggcgtttgcg
    11581 caggcctcgc tggcgcaggc cgtggaactc gccctgtgca cctgggccgt cggttcgtta
    11641 gcccaagcgg cgtccgagtc cccggctgcg gagtcatctt caacccagct atcggaagaa
    11701 cagacggaag gggacggttc gagtctccct gacaaggcgg cgttgctggc gcatgtgcgt
    11761 caaaccgtcg ctgaaaagtc attggaatgt gtcctgctgg agcgccgagg gcctggatcg
    11821 gccaaatatc gcgcgcttcg tcctgaggaa ctgggccgtc tgttgccttc ggctgtcagt
    11881 tcaccggcca ccacctgata tgttgaatcg tatcttcgga cttgaaacgg aatacggcct
    11941 cctggtgaac caggatcgtc ccgaccattc gccctcctgg gtggcgcagc gtatccgcga
    12001 ccacattttt catgtcgaac ggcgaggagt cctcgatctt caccatcgtg gtcatgacga
    12061 gccgccgggc aacggggggt ttctgaccaa tgcgggccgt atctacatcg atatggggca
    12121 tctggaatat gcctctccgg agtgcacgac cctggcggat cttgtggcct cggatcgtgc
    12181 aggggatcgg attattcaag atgcggtgag ggcgctgggg ctggacgaga cggtgtcgct
    12241 catcaagaac aatatcgatc acgagaccga tgcgacgttc ggctcgcatg aaaactatct
    12301 cgtcacgcgg cagtttccct tttcccggcg cggcctcggt ccactggtga cgttcttggt
    12361 cacccgtcag gtgttcaccg gggcggggcg catcggatgc gcgagtgatc ccaacgagtg
    12421 ggtgcaagtc gggggcctca tcctgcatcg gcctgggctg cgggatgcgc aagatcgatc
    12481 gattgtgccg ttccagatct cccaacgcgc cgactacatc gtcaacgact ttttcgagtg
    12541 ggttcaacac aaccgtgcga tcgtcaatac acgcgatgag ccgctggccg atccgaacca
    12601 ataccgccgg attcatctcc tgtgcgggga ttcgaacatg gccgaatatg ccacggcgct
    12661 caagatgggg accaccgggc tggtgctgca actgattgag gccggacagg ccccgcgcgg
    12721 cctggggatc cacgagccgg tcgaggccct gcaggatatt tcgcgcgacc cggatcgccg
    12781 ttggatcgtc cgtttggagt cgggacagtc gatgtccgcc atcgatattc aggaacagtt
    12841 tctggccgcg gcccagcgcc attgcaaggg gcaggacgaa gagacggatt gggtgctgga
    12901 gcaatgggaa tccgtgctga ccgatttgcg tggcgggtat gaaaagctgg tcggccggat
    12961 cgattgggcc tcgaaactgt ggctattgga gacctatcgg gaggccgagc agtgtgcgtg
    13021 ggacgatccc atgttgaaga gtctcgacct cgaatatcac aatctgcatc ctgagcgagg
    13081 actctgctac ggtctcgagg aggaggggcg aggaccgcgc ctgacgaccg ataagatcgt
    13141 tcatctcgcg caggaccatc cgcctcgcaa cacgcgggcg ttcggacgcg gtgagttggt
    13201 caggcatcta ctggccagcg gaggcggagg gatcgtgtcg gatgccccgc tcgacttcga
    13261 agaacagatg gcctgcgagt acatcatcaa ttggtcgaat tttaaactgc gcggaacggc
    13321 gccgtttttt atggctgacc cgttcaaaac ctacgtgcag gatgtgcgta gccacctcgc
    13381 cgctcgatag tctggcagga tgcggaaaaa gtccgccagc ggcgttctcg cggcgctcag
    13441 aggctcaccg tacggcgcga gtacgattcg tctcttcgct tactgcggcc ttgctggacg
    13501 gcctttttgc gcatcctgcg ggttag
//
LOCUS       DAOQRM010000061        13525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3619_length_13525_cov_17.404826, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000061 DAOQRM010000000
VERSION     DAOQRM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13525
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3619_length_13525_cov_17.404826"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /gene="gmhB"
                     /locus_tag="PKK81_06695"
     CDS             <1..797
                     /gene="gmhB"
                     /locus_tag="PKK81_06695"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNV32597.1"
                     /translation="TTSPFGVDAQLVRQPVSCAPCLLRECPIDHRCMTGVTVEQVYDA
                     VVQHLPLVAPPPAVDPAPPTPGLTSTSLAGVTVFLDRDGTLNVDTGYVKSPDDFTVLP
                     GVGAALARLKQAGARLVVVTNQSGLGRGYFSSRDLEAIHSKLRLVLAEDGVTLDGLYF
                     CPHHPDDHCNCRKPARGMIDRAHAELKVDLSRSYVIGDSIRDIELAKQVGARSLLVMT
                     GPSSAEALADLTVRDLPPDYVAEGLSQAVDWIVAHATHRPAADMQR"
     gene            815..1858
                     /locus_tag="PKK81_06700"
     CDS             815..1858
                     /locus_tag="PKK81_06700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV32598.1"
                     /translation="MTDSVRRLLLIKPSSLGDIVHAMPTLAALREQFPQARVTWLVKR
                     QWAPLVEVIAGVDQVCSVSEGLRGWLGRVPDLRAAGFDLVVDLQGLFRSGAMARLSGC
                     DRRIGFANAREGSSLFYTQRVAVPPAPMHAVDRYLLVAEALGATRPTQPRFEFVDRAE
                     DRQAVETMLSRAGVAPHQPWVAMNVSARWETKRWPPQHFAEAADRLSQAHALPVVLIG
                     GPAERAESLAVTALMRTKAIDLTGQTPVGLLPGLLRRASLLVTNDSGPMHIAAAVGTP
                     VVALFGPTDPVKTGPYGKGHVVLSHAVECRPCFRRDCARAVPLECLTAVRPEQVVRAV
                     EQQLERSQKPGSL"
     gene            1855..2958
                     /locus_tag="PKK81_06705"
     CDS             1855..2958
                     /locus_tag="PKK81_06705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV32599.1"
                     /translation="MNIIIAESSTAVGGQELAVLLHAERLLKRGHRIRLVLEPRSPIM
                     AMARERGLPVEPFVMRQWRLPLSILAFRALLKRERPEIVHVNSSRDSWIAALSSRLLD
                     PRPKVIRTRHISAPLNNNATTHWLYRRLFDMVIVTGSERNRQDLIHRDGLAPDRVASF
                     PIGLDVEHFSPAKPQHDIRSELGIPTGHLLVGMISYLRDYKGHRYLVEAAAKVLKQHQ
                     GVAFLIVGEGPEEQNIRAQIERLGLTAGVRMLGFRDDLLDVFRSLNLFVIPTVEGDTI
                     PQVLMQALAIGLPVVSTTTGSIPDVLADGESGFIVPPRDADALADRIGRLLVDPELRA
                     AMGRRGRQTVEQSYSIDRMVDELERVYRRVIAS"
     gene            2955..5096
                     /locus_tag="PKK81_06710"
     CDS             2955..5096
                     /locus_tag="PKK81_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNV32600.1"
                     /translation="MMQRWAEARASIRKSVLFWWILFVVMQMAERVFLLRDAFEQEAP
                     SLPLLLKTLVVGVRGDFITATFALVLAGVGAGAWALRGHGWSGLRRLTQPFLLSFRSG
                     LHVGCVLTGLLLFVLLCVDMGYYGFNRQHMDFVFLEYVGDLFAPAATSDSTNVQAMQQ
                     TSAELGAAGKWAGRLALFLGIQAVAMGLWWWSFSCAIVPALARWRPGSGFQANTFLLV
                     ALVAGGAGFHHSGPYGIRIAPIGSMVYYTLAQNPVLFAAEALRIAVVSRGSMEQRASA
                     EAMPYEVAVQTVQRLLGPGESFVDQRYPLVRTMAASSDGVRLPGQANILLVFLEGLDR
                     RYLEQKYGEIQGTPFLDRLKQESVYFENFFSNGVQTSRGLFATLCSAFPRQGAAAMKT
                     RYAHDYLCLPSLLQRQGYRTEMVIGQHRDLNRLQSFVARNGLQQLIDEGDFPKDAERM
                     GLGIVDGALFDLCYERIKQRQAEDKPFFLTTLTLSTHHPFAAPQNHPDVRLLQEQVQD
                     KYVGALRYTDLEFERVFTRLVREGLLRNTVVVVLGDHGRHEPVGSTDMERKAGHFASP
                     LFIWMDESLRTPATYRPRTVSAIASQVDVAPTLLALNGILPAVGAFAGHDLTCLLVRD
                     CLPNQVAYLTSVYDNLVGLASPDGLLLYSFSTESFQEATLNFDAVSDSESGRRRRFAT
                     HDRELMALYEVANVALDSNRLWSWREFGTRL"
     gene            5149..5946
                     /locus_tag="PKK81_06715"
     CDS             5149..5946
                     /locus_tag="PKK81_06715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV32601.1"
                     /translation="MNHIPVSAVVIAYNDEPNMRACLESITWADEIIVVDSHSTDATE
                     RISREFTDQVYQHDFHGFGRLRNEALTHATHDWVFSLDTDERATPELREEIRRVLAAG
                     PEADAYFVPRKNYFLGRWIKHCGWFPDYRQPQLFRKSRLRYREELVHESFDLDGKIGY
                     LTAAALQYPFRDIDHYLAKQERYSDLMARRMVEQGRAFSPHQLVSHPCFTFLKMYVGR
                     KGCLDGVPGLILSGLYAYYTFIKYARFWELQSRVLPEGSQAAPVKQG"
     gene            5949..6746
                     /locus_tag="PKK81_06720"
     CDS             5949..6746
                     /locus_tag="PKK81_06720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV32602.1"
                     /translation="MPVTDDGARRRTMTIAAVVITKDEEKNIADCLESLRWADELIVV
                     DAESRDRTVELAKQYTPKVFVRPWPGYGPQKNFGIDQAAAEWILVVDADERVTGGLRR
                     EIDALLATAPAADIGGYEIPRRNFFYGKWIQGGGLYPDYQLRLFRNTAGRYDDVKLHE
                     NFTLSGRRERLREPFDHYSMPTVNHHIRKMMRYTTLGADEKLKRVTHISGWVIATHHL
                     GTILKTLFSRGGYRDGVHGLVVAMFAGLHTFVKYAKAWERVNVRRDA"
     gene            6796..7899
                     /locus_tag="PKK81_06725"
     CDS             6796..7899
                     /locus_tag="PKK81_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNV32603.1"
                     /translation="MRIGIDAASIVGDKGGVGWHTYHLLRSLLALDEQVEYVGYLRPG
                     SLQAGRLEGWPVHDRLRWVETPRWLMPCRGAWDRLDLYHGPNFKMHTTGRSGGIVTIH
                     DLWLVRHPEYSRKLLGQAGSSRRAIATANRARRVVTVSEFSAREIEALYGIPRERVVV
                     IHNGVTEEFSPVQDEQGMAVLRARWAIPSAGFILFVGGADPRKNHRVFLQAVAQSRSQ
                     LGGRAILLVGDAEHPQGSYLATARSLGLEQDVRCTGRLNREDLCRLYSYAEVFVFPSL
                     YEGFGMPVLEAMACGAPTITSSTSSLPEVAGDAALLVNPEDAEALGAAMVKVLSEQAL
                     RQQLRDRGFERARLFTWQRAALRTSALYRELCA"
     assembly_gap    8215..8314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8315..9072
                     /locus_tag="PKK81_06730"
     CDS             <8315..9072
                     /locus_tag="PKK81_06730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV32604.1"
                     /translation="LATGAPVRIGFNAEHRWRGLLYSTVAKPRSTDQHRVDYDLCALR
                     ALGLDTKPGTPALYASPADEQAVEAWLREAGLLSAQSSQLLILLQPGARYSLKVWPHE
                     RFAQLADRLADRFACRILLGGDQREREVAEQVARKTRCAPIVVAGKFSLLQFAALVKR
                     CALFVGNDGGAMHIAATMGTPVVAIFGPTYPQRWGPRGGPAQVIYKGLDCRACYHPTC
                     LRGDDSCMQQIAVDEVFTAASRMLERTPARTET"
     gene            9069..9968
                     /locus_tag="PKK81_06735"
     CDS             9069..9968
                     /locus_tag="PKK81_06735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV32605.1"
                     /translation="MSDELVSVVIPVFNGAPFVAKAVASVRSQGHGAVEILVVDDGST
                     DGTQEVLKRLEQSDGIRWFQRSHGGPARSRNYGIAAAQGQFIALLDCDDIWLPGKLAA
                     QLAIMRARPDVGLVHTDFEVRFEDGTLEERVSARSSREPMVQAFAGGHVALPSTLLIR
                     KSVLDQIGSLDPELYGSEDSDLTIRLFRVTRFECIGEVLVHKLQRGHGYRDMAFDEQT
                     HRERVLASRDRFLMRLESFAPLTAAQRAALDREWANYFLLKGAAAERAGRRSDARRHY
                     WEAICKAPARVRCYTRWLRTLKP"
     gene            10036..11052
                     /locus_tag="PKK81_06740"
     CDS             10036..11052
                     /locus_tag="PKK81_06740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV32606.1"
                     /translation="METISCDVCGEASNTALFCQYDLTHHVTDDLFTVVRCQGCRLLF
                     LNPRPTREEIGGYYPSTYYPEAAPRQAGDLRRTAKRWSGKIRRWIAQDFYGYPAPESA
                     RRWQWARRLLLWPEFLWRRWRGRGLLPWAGQGRVLDVGCGSGGNLAVLQEQGWNVSGV
                     DASAVAVAQAQARFGERVRQGDLASMAYPERTFDTVLFSHVLEHLHDPLSLLKEVWRI
                     LDWEGRVVILCPNAGSLEARMFGRWWFPWELPRHLFHYERATLTRLLEAAGFQIESVS
                     TGLGSLYFMASLDRLCEERFKRAVPWRRLIEKVLIRPFCFCVGHLGHGTELKVYARKG
                     RSAG"
     gene            complement(11049..11960)
                     /locus_tag="PKK81_06745"
     CDS             complement(11049..11960)
                     /locus_tag="PKK81_06745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNV32607.1"
                     /translation="MLPIDASKELTPVAQISVVIPAYNGTSRYLKEAIDSVRAQTLAA
                     GEIIVVDDASTDGTGALVQAIPQVRYVRHPQNSGQAAARNTGARLAQTPYLAFLDQDD
                     LWEPTFLEATLGILESAPQATLVHCDGYQVNERNEILEYDAAMKQQRSITQLLRGGHD
                     AATSGSLFRKTCFDAVGGYDAALSIWEDIDLIIRLYGPGRFIHLPKPLYRHRLYGHNA
                     SRNIPSLRALDGRRRFLDKHAASCRPGTPEGDALSHDWAIYYADLGKHHLRANTPTAA
                     RQAFQSALRHDPYNVKTWSRLVRSYFV"
     gene            12178..13257
                     /locus_tag="PKK81_06750"
     CDS             12178..13257
                     /locus_tag="PKK81_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNV32608.1"
                     /translation="MKIVIAAWHLKNLNVGIGRYARELIEALGRVDQTNHYEILIPHA
                     EHPFTARPNMRYRVIRFPLFRRRFWEQVAPLLVGPYDVLHFPYDSCVAWKRGKFVATI
                     HDVKPLLFPVLRARTNLNSRIEQWLVGDRWKKLDHVITISEHSRRDLLAHVPLRPEQV
                     TVTSLGLDAERFRPAEHPGERKPYVFCVAGADPTKNVGVLIDAFAKLPEMLRSRFDLI
                     LAGDVCKRQDIRAAVENHGLSAHTTLVGVVSDRELVAYYQQATLFVFPSLYEGFGLPV
                     LEAMGCGCPVICSNASSLPEVAGDAAVLFDPRDGGRLALELSRVLASPALRDELRARG
                     VARAREFSWDRTAIETVAVYRRLAG"
BASE COUNT         2300 a         3806 c         4477 g         2842 t
ORIGIN      
        1 aaaccacgtc gccgttcggc gtcgatgccc agctggtccg gcagccggtg tcctgcgcgc
       61 cctgtctctt gagggaatgt cccatcgatc accggtgcat gaccggggtg acggtggagc
      121 aggtctacga tgcagtggtg cagcacctgc cgctggtggc ccctcctccg gcagtcgacc
      181 ccgcgccacc gacgccaggt ctcacttcca cgtcgctggc gggtgtgacc gtatttctcg
      241 atcgcgacgg cacattgaac gtcgatacgg gttatgtgaa gtctcccgat gacttcacgg
      301 ttctacccgg cgtgggagcg gcgttggccc ggttgaaaca ggccggcgcg cgcctggttg
      361 tcgtcacgaa tcagtcgggg ctcgggcggg gatatttttc atccagagat ctcgaagcga
      421 ttcacagcaa gttgcggctg gtgctggctg aggatggggt gacgctcgat ggactgtatt
      481 tttgtcccca ccatcccgac gatcactgca actgccgcaa accggccagg ggcatgatcg
      541 accgtgctca tgccgaattg aaagtggatc tgagccgatc ctatgtgatc ggtgacagta
      601 ttcgtgatat cgaactggcc aaacaggtcg gggcgcggag cctgctggtc atgaccggcc
      661 cgtccagtgc ggaggccctg gccgacttga cggtgcgcga tctgcctcct gactacgtgg
      721 ccgaggggtt gtcgcaggcg gtggactgga tcgtcgccca tgcgacgcac aggcccgccg
      781 ccgacatgca gcgctgatca tctctggtgg ccgaatgacc gactccgtac gccgactcct
      841 gctgatcaaa ccgagttcgc tgggcgacat cgtgcatgcg atgccgaccc tggcggcgtt
      901 gcgcgagcag tttccccaag cccgggtcac ctggctggtg aagcgacaat gggcgccgct
      961 ggtggaagtc attgccgggg tcgaccaggt ctgttcggta tcggaaggac tcaggggctg
     1021 gttggggcgg gtgcccgatc tccgggcggc cggcttcgat ctggtcgtcg atcttcaagg
     1081 actcttccgg agcggggcga tggctcggtt gagcggttgc gaccgtcgga tcgggtttgc
     1141 caatgcgcgg gagggcagct ctctgttcta tacccagcgg gtggcggttc ctcccgcgcc
     1201 catgcacgcg gtcgatcgct acctgctcgt cgccgaggcg ctaggcgcga cacgtccaac
     1261 gcagccgcgg ttcgaattcg tcgatcgagc cgaggaccgg caggccgtgg agaccatgtt
     1321 gtcccgggcc ggagttgccc ctcatcaacc atgggttgcc atgaacgtgt ccgcacgttg
     1381 ggagaccaag cgttggccac cgcaacattt tgctgaggca gcagaccggc tttctcaggc
     1441 gcatgcgctg cctgtggtcc tgatcggcgg gccggccgag cgggcggagt cgctcgcggt
     1501 gacggcgctc atgcgtacga aagcgattga tctgacagga cagacccctg taggcttgct
     1561 ccccggttta ctccgtcgcg cgtccttgtt ggtgacgaac gattccgggc ctatgcacat
     1621 cgctgcggcg gtggggacgc cggtggtggc cctgttcggc ccgacggatc cggtcaaaac
     1681 cgggccctat ggcaagggcc atgtcgtcct gtcgcatgca gtggaatgca gaccctgttt
     1741 tcgccgtgac tgtgcccgtg ccgtgcccct cgaatgtctg accgccgtgc gaccggaaca
     1801 ggtcgttcgt gccgtcgaac aacaacttga gcgatcacag aaaccaggat cattgtgaac
     1861 atcatcattg cggaatccag cacggcggtc ggcgggcagg aactggccgt tctgctgcac
     1921 gcggagcgcc tgttgaagcg cggccatcgc attcgattgg tcctggagcc gcgcagcccg
     1981 atcatggcca tggccaggga acgagggctt ccggtcgaac cctttgtcat gcggcaatgg
     2041 cggctgccgc tgtcgattct ggctttccgc gcgttgctca agcgggagcg tcccgagatc
     2101 gtccacgtca acagttcacg cgatagctgg atcgcggcgc tgtcgtcgcg cctgctcgat
     2161 ccgcgcccca aagtcattcg cacgcggcat atttcggcgc cgctcaataa caacgccacc
     2221 acgcattggc tgtatcgtcg tctcttcgac atggtcatcg tgaccgggag cgaacggaat
     2281 cggcaggacc tcatccatcg ggacggattg gcgccggacc gtgtcgcttc gtttcccatc
     2341 ggcttggatg tggagcactt ttcgccggcc aagcctcagc acgatatccg gtccgagctg
     2401 gggattccca ccgggcatct gctggtcggg atgatttcct atctgcggga ctataaaggc
     2461 caccggtatc ttgtagaggc ggccgccaag gtgctgaagc aacaccaggg agtggccttt
     2521 ctcatcgtgg gtgaagggcc ggaagagcag aacatccgcg cgcagatcga acgcctcggc
     2581 ttgaccgccg gcgtgcgcat gttgggattc cgagatgacc tcctggatgt gttccggtcg
     2641 ttgaatctct ttgtgattcc cacggtcgaa ggcgatacca ttcctcaggt gttgatgcag
     2701 gccctggcga tcggactccc ggttgtctcg acgaccaccg gatcgattcc cgatgtgctg
     2761 gccgacgggg agtcggggtt catcgtcccg ccgcgggatg ccgatgcgtt ggccgatcgg
     2821 atcggtcgcc tgctggtcga tcccgagctt cgcgccgcga tggggcgacg gggccgtcag
     2881 acggtggagc agtcctattc gatcgaccgg atggtggatg agctggagcg tgtctatcga
     2941 cgggtgatcg catcatgatg caacgatggg cggaagcccg cgcctccatc cgcaaaagcg
     3001 tgctgttctg gtggattctc ttcgtcgtga tgcaaatggc ggagcgggtg tttctgttgc
     3061 gcgacgcgtt tgagcaggaa gctcccagcc tgccgctgct gctcaagacc cttgtcgtcg
     3121 gcgtgcgggg ggatttcatt accgccacct ttgcgttggt actggccggg gtcggggcag
     3181 gggcatgggc cctgcggggg catggctgga gcggattgag acgtctgacc cagcccttcc
     3241 tcctgtcgtt tcgatctggg ctgcacgtgg ggtgtgtgct gaccggcttg ttgttgtttg
     3301 tcctgctgtg tgtggacatg gggtactacg ggttcaaccg gcagcatatg gacttcgtgt
     3361 ttctcgaata tgtcggcgac ctctttgcgc ctgcggcgac ctcggacagc accaatgtgc
     3421 aggcgatgca gcaaaccagt gcagagttag gcgcggctgg taagtgggcg ggaaggctgg
     3481 cgctctttct gggcatccag gctgtggcca tggggctgtg gtggtggagt ttttcctgcg
     3541 ccattgtgcc ggcgttggct cgctggcggc cgggctcggg gtttcaggcg aacacgttcc
     3601 tccttgttgc gctggtggcc ggcggcgccg ggttccatca ttccggcccc tacgggattc
     3661 ggatcgcgcc gatcggaagc atggtctatt acacgctggc gcagaatccc gtcctctttg
     3721 cggctgaggc cctacgaatc gcggtggtat cgcgagggtc gatggagcaa cgggcaagtg
     3781 ccgaggcgat gccttatgag gtcgccgttc agacggtgca gcgattgctc ggcccgggtg
     3841 aatcgtttgt ggatcagcgg tatcccctgg tcagaaccat ggccgcctct tctgacggtg
     3901 ttcggttacc cgggcaggcg aacatcctgt tggtatttct cgaagggctg gatcgccggt
     3961 atctcgagca gaagtacggc gagattcagg ggacaccttt tttggatcgc ctgaagcagg
     4021 agagcgtcta cttcgagaac tttttctcca acggcgttca aacgtcacgg gggctgtttg
     4081 cgaccctctg cagcgccttt ccgcggcaag gggccgccgc gatgaaaacg cgctatgccc
     4141 acgattacct ctgtctgccg tctttgttac agcgtcaggg ctaccgaacg gagatggtga
     4201 tcgggcagca tcgggacttg aaccggcttc agtcgttcgt ggcacgcaat ggtttgcagc
     4261 agctgatcga cgagggagat tttcccaagg atgcggagcg gatgggcctc ggcatcgtgg
     4321 acggcgccct gttcgatctc tgttacgaac gcatcaagca acgccaggcg gaggacaaac
     4381 cgttttttct gacgaccctc acgttgtcga cccaccatcc cttcgcggcc ccgcagaacc
     4441 atcccgacgt gcgtctgctg caggagcagg tgcaggacaa gtatgtcggc gctctgcgat
     4501 atacggatct cgaattcgag cgggtgttta ccagactcgt tcgcgaggga ctcttgcgga
     4561 atacggttgt cgtcgtcctg ggtgaccatg gccggcacga accggtcggg agcacagata
     4621 tggagcgaaa ggcgggtcat tttgcatcgc cgctgtttat ctggatggac gagtcgttgc
     4681 gcactccggc cacctatcgc ccgcggaccg tgtcggccat tgccagccag gtcgatgtgg
     4741 ctccgaccct gctggcgctc aacggcattc tacctgccgt gggggcgttc gccggacatg
     4801 atttgacctg tctgttggtg cgggattgtt tgccgaacca ggtggcctat ctcaccagtg
     4861 tctacgacaa tctggtcggc ctggccagtc cggacgggct gttattgtat tcgttctcga
     4921 ccgagtcgtt tcaagaggcc acgctcaatt tcgacgcggt atccgacagc gagtcgggcc
     4981 ggcgacgacg gttcgctact cacgaccggg agttgatggc tctctacgag gtggcgaatg
     5041 tcgcgctcga ttcgaatcgc ctctggtcct ggcgcgagtt cgggacacga ttatgatcgt
     5101 gtggggcaca atttcttgtt cgtcgttgtt cgagaaggat ctgctgctgt gaatcacatt
     5161 ccggtgtcgg ccgtggtcat cgcctacaat gacgagccca atatgcgggc ttgtctggag
     5221 tccattactt gggcggatga aatcatcgtg gtggattccc acagtaccga cgccaccgag
     5281 cgcatcagcc gggaatttac cgaccaggtc tatcagcatg actttcatgg attcggccgt
     5341 ttgcggaacg aggccctgac gcatgccacg cacgactggg tgttcagtct ggataccgat
     5401 gaacgggcga cgccggagtt gcgcgaggag atccggcgtg tgctggctgc cggcccggag
     5461 gccgacgcct acttcgtccc ccgcaagaat tactttctcg ggcgctggat caaacattgc
     5521 ggctggtttc cggactaccg gcagccgcag ttgtttcgga agagccggct gcgttatcgg
     5581 gaggaacttg tccatgaaag tttcgatctg gacgggaaaa ttggctactt gacggccgct
     5641 gcgctgcagt accccttccg ggatatcgac cattacttag cgaagcagga gcgctattcc
     5701 gatctcatgg cgcggcgcat ggtcgagcag ggacgtgcat tctccccgca tcagctggtc
     5761 tcacatccct gtttcacatt tctgaaaatg tatgtcgggc ggaaaggctg tctggacggg
     5821 gtgcccggac tcatcttgtc cggactctat gcctattata cgttcatcaa gtacgcgcgt
     5881 ttctgggagt tgcagtcccg ggtgctgcct gagggaagcc aggctgcgcc cgtaaagcag
     5941 ggctaagcat gccggtcacc gacgatggag cgagacgcag aacgatgacg attgcggcgg
     6001 tagtcatcac caaagatgaa gaaaagaata ttgccgactg tcttgagtcc ctgcgctggg
     6061 ccgacgaact gatcgtcgtg gatgcggaaa gccgggatcg gacggtggag ttggcaaagc
     6121 agtacacccc gaaagtgttt gtccgcccgt ggcccgggta tggcccgcaa aaaaacttcg
     6181 gcatcgatca ggccgctgcg gagtggattc tcgtggtgga tgccgacgag cgggtgacag
     6241 ggggattgcg acgggaaatc gacgcgttac tggcgacggc cccggccgcc gacatcggag
     6301 ggtatgagat tccccggcgg aattttttct acgggaaatg gattcaaggc ggcgggctct
     6361 atccggacta tcagctgcgg ctcttccgaa acactgccgg ccggtacgac gatgtgaagc
     6421 tgcatgaaaa cttcacgctc agcgggcgtc gggagcggct tcgggagccc ttcgatcact
     6481 acagcatgcc caccgtcaac catcacatcc gcaagatgat gcggtatacg acgctcggtg
     6541 ccgatgagaa actcaagcgg gtcacccata tcagcggctg ggtcattgcc acgcaccatc
     6601 ttggtacgat cctcaaaacc ctgttctccc gcggcgggta tcgcgacggg gtgcacggat
     6661 tggtggtggc gatgtttgcc ggcctccaca cattcgtcaa atatgccaag gcctgggagc
     6721 gtgtgaacgt gaggcgggac gcatgatcga tccaaccttg cccggcatga acctggcgga
     6781 ggtcccgttc ccctgatgcg tatcgggatc gacgcagcgt caatcgtggg ggacaagggc
     6841 ggcgtcggct ggcataccta tcatctgctc cggtcgctgc tggccttgga tgagcaagtc
     6901 gagtatgtcg gatatctccg gcccgggtct ctgcaagcgg gccggctcga aggctggccg
     6961 gtccacgatc gtctgcggtg ggtggaaacg ccgcggtggt tgatgccatg ccggggcgcg
     7021 tgggacaggc tcgatctcta tcacggcccc aacttcaaga tgcatacgac gggacgatcg
     7081 ggcggcatcg tcaccatcca tgacctgtgg ctggtccggc acccggaata ttcgaggaag
     7141 ctgttggggc aggccggttc ctccaggcgg gccatcgcca cggccaaccg ggcgcgacgg
     7201 gtcgtgacgg tgtcggagtt ttcagcccgt gaaatagaag ccttgtatgg aattccgcgt
     7261 gaacgggtgg tcgtcatcca taacggcgtg accgaggaat tctctccggt tcaggatgag
     7321 caggggatgg cagtgttgag ggcacggtgg gcgattccat ctgccggatt tatcctgttc
     7381 gtcggcggag cagatccacg aaaaaaccac cgtgtttttc tgcaggccgt ggcacagtcg
     7441 cgttcgcagc ttggagggcg ggccattctg ctcgtgggag atgcggagca tccgcaggga
     7501 agttatctcg cgacggcccg gtcgctcggg ttggagcagg acgtgcgttg caccggtcgc
     7561 ctcaatcggg aggatctgtg ccggctctac tcttacgccg aggtgtttgt ctttccgtcg
     7621 ctctatgagg gcttcggcat gccggtgctg gaggcgatgg cttgtggagc gccgacgatt
     7681 acctcctcga cgtcgtctct tccggaagtg gcaggggatg cggcgttgct ggtgaaccca
     7741 gaggatgccg aggcgttagg agcggcgatg gtgaaggtgc tctccgagca ggcgctgcga
     7801 cagcagcttc gggatcgtgg ctttgagcgg gcgcgactct ttacctggca acgtgcggcg
     7861 ttgcgcacca gcgccttgta tcgtgaactc tgtgcctgag gcaggctgtt gaaaaaaacc
     7921 gccagcatcg ttctcacttc gctcagaggt tcagcgtacg gccgagagta cgctgaacct
     7981 cttcgcttgc tgcggccttg ctgggcggtc tttttgaaca gcctgcaggc tatgagtgcc
     8041 ccatatgcga ttctcagtag ttctgaattc caaaggtgtt gggatagtgt tgcagcgagc
     8101 tgctaagagc gacggcgtga tggatcttca gaatattctg atcatcaaat tgcggcatat
     8161 cggcgatgtg ctcctatcta cgccggtgtt gcgcggactg cggactgcct ttccnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtctgg cgaccggtgc gcccgtccgt
     8341 atcggattta acgccgaaca tcgctggcgc gggctcttgt acagcaccgt ggccaaacca
     8401 cgctcgacgg atcagcatcg agtggactac gatctctgcg ctctgcgggc cctggggctg
     8461 gacacgaagc cgggcacacc ggcattgtat gcgtcgccgg cagatgagca ggcggtggag
     8521 gcctggctgc gggaggccgg gctcttgtcc gcgcaatcct ctcagttgtt gatattgttg
     8581 caaccggggg cgcgctactc gctgaaagtc tggccgcatg aacggtttgc ccagttggcc
     8641 gatcgtctcg ccgacaggtt tgcctgccgc attctcctgg gcggcgacca gcgtgaacgc
     8701 gaggtggcgg agcaggtcgc ccgcaagacg cgctgtgcgc ctatcgtggt cgcagggaag
     8761 ttttcgctct tacagtttgc cgcgttggtc aaacgttgtg cgctgttcgt cggaaacgac
     8821 ggtggcgcca tgcacattgc cgcaacgatg ggaacgccgg ttgtggctat tttcgggccg
     8881 acctatccgc aacggtgggg gccgcgcggc ggaccggcgc aggtgattta caaaggcctg
     8941 gattgccggg cctgctatca tccgacctgc ttgcgcgggg acgatagctg tatgcaacag
     9001 attgcggtgg atgaggtgtt tacggcggcc agccggatgt tggaacgcac gccggcgagg
     9061 acagagacat gagcgacgaa ctggtcagcg tggtcattcc ggtgttcaac ggtgcgccgt
     9121 tcgtcgccaa ggccgtggcc agcgtgcggt cgcaggggca cggtgccgtg gaaatcctag
     9181 tagtggatga cggatcgaca gacggaacgc aggaggtgtt gaaacgtctt gagcaaagcg
     9241 acggcattcg atggtttcag cgcagccacg gcggaccggc ccgttcccgg aattacggca
     9301 tcgccgcagc gcagggacag ttcatcgcgt tgttggattg tgacgatatc tggttgccgg
     9361 gcaaactcgc cgcccagttg gctatcatgc gtgcgcgacc ggacgtgggg ctggttcata
     9421 cggacttcga ggtacgattt gaggacggca ccttggagga gcgcgtgtcg gcacgatcga
     9481 gccgtgagcc gatggtgcag gcgtttgccg gcggtcatgt tgcgctgccg tcgacactgc
     9541 tgattcggaa atcagtgctc gatcagatcg ggagtctgga ccctgaactg tacgggtcgg
     9601 aagattccga tctcacgatc cggctgtttc gtgtgaccag gttcgagtgc atcggtgaag
     9661 tgttggtgca taagttgcag cgggggcacg gctatcggga catggccttc gatgagcaga
     9721 cccaccggga gcgggtgttg gccagccgcg accgctttct gatgcgcctc gaaagcttcg
     9781 cgccgctcac ggcggcgcaa cgcgccgcat tggaccggga atgggcgaac tactttctat
     9841 taaaaggggc ggcggcagaa cgggccggcc ggcggagtga cgcgcggcgg cactattggg
     9901 aggcgatctg caaggccccg gcccgcgtgc gatgttatac gcgctggctt cgcaccctga
     9961 agccctagcg acagcgccgg gtatgcccca caggccttgc gccgagcacc tcgcaaagag
    10021 atcgccaaag gatctatgga aaccatcagt tgcgacgtat gcggagaggc ctcgaacaca
    10081 gccctctttt gccaatatga tctcacacat cacgtcaccg acgacctatt tacggtcgtt
    10141 cgttgtcagg gctgccgcct gttgttcctg aatcctcgtc ccactcgcga ggagatcggc
    10201 ggctactatc cttccaccta ctatcccgag gcagcgcctc gtcaggccgg cgatctccgc
    10261 cgcactgcca aacgatggtc gggaaaaatc cggcgatgga tcgcccagga tttttacggt
    10321 tacccggcgc cggaatcggc ccgtcgctgg caatgggcgc gccggcttct gttatggcct
    10381 gagttcctgt ggcgccgttg gcgggggcgc gggctgctcc cctgggctgg gcaggggcgg
    10441 gtgttggatg tcgggtgcgg ttcgggaggc aatctggcgg tcctgcaaga gcaaggatgg
    10501 aacgtgtcgg gggtggatgc cagtgccgtg gcagtggcac aggcgcaagc tcgtttcggt
    10561 gagcgagtcc ggcagggtga tctggcgtcg atggcctatc cggaacggac gttcgatacg
    10621 gtcctgttca gtcatgtcct ggagcattta cacgatccgc tgtccctact caaagaggtc
    10681 tggcgaatat tggattggga ggggcgggtc gtgatcctct gtccgaatgc cggcagcctg
    10741 gaagcgagaa tgtttggacg atggtggttt ccatgggaac tgccacgcca tctgtttcac
    10801 tatgaacgcg ccacgctgac gcggctgttg gaggctgccg ggttccagat tgagtcggtc
    10861 agcaccggtt tgggatcgct ctactttatg gcgagcctgg accggttgtg tgaagagcgg
    10921 ttcaagcgag cggtgccctg gcgccggctg atcgagaaag tgctcatccg cccgttttgt
    10981 ttctgcgtcg gccatctagg gcatgggact gagttgaaag tgtatgccag gaaaggtcgg
    11041 agcgcgggct agacaaaata cgatctgacc agacgcgacc atgttttgac attgtaggga
    11101 tcgtggcgga gggctgactg gaacgcctga cgagcggcgg tgggcgtgtt ggcccgcagg
    11161 tggtgtttgc cgaggtcggc ataatagatc gcccaatcgt gcgagagggc atcgccttcg
    11221 ggggtgccgg gtcggcaaga ggcggcgtgt ttgtcgagga accggcgccg accgtccagc
    11281 gcgcgcaacg aggggatatt ccgcgacgca ttgtgtccgt agagccggtg ccgatagagc
    11341 ggttttggaa gatgaatgaa tcggcccgga ccgtagagcc ggatgatcag atcgatgtcc
    11401 tcccaaatcg aaagtgccgc atcgtagccg cccacggcat cgaaacaggt cttgcggaaa
    11461 agcgagcccg atgtggcggc gtcgtgtccg ccccgcagca attgcgtgat gctgcgctgc
    11521 tgtttcatcg cggcatcgta ttcgagaatc tcgttccgtt cattgacctg gtacccgtcg
    11581 cagtgcacga gggttgcctg cggagcgctc tcgaggatgc cgagcgtcgc ttccaggaag
    11641 gtgggctccc acagatcgtc ctgatcgaga aatgccagat agggggtttg ggccaggcgc
    11701 gcgccggtat ttctggcggc ggcttgtccg ctgttctgcg ggtgacgcac gtagcggacc
    11761 tgcgggatgg cttgcaccaa cgcgcccgtg ccgtccgtcg aggcatcgtc tacgacgatg
    11821 atctcaccgg cggccagggt ttgtgcgcgc acggaatcga tggcctcctt cagatatctg
    11881 gaggtgccgt tgtaggcggg aatgacgacg ctgatctgtg ccacgggcgt gagttccttg
    11941 gaggcgtcaa tcggtaacat aggcagtgaa cccagtcaac cggcgctgct gccgacccat
    12001 ccagacgaca tcgattcgac tgtcttgact tgccgggtag gctgtgattc catgcgcgcc
    12061 gattgcgtgc gggtggttgt tcgtgatgcc atcgcgccca atcgttcgtg tgatggcccc
    12121 tgtgcgtgat cgcgagccgg tcctttcctt caccataggt ctattccgcg caatattgtg
    12181 aagatcgtca ttgccgcatg gcatctcaag aatctcaacg tcgggatcgg gcgatatgcc
    12241 cgggaactga ttgaggccct ggggcgcgtc gatcagacga atcactacga gattctgatc
    12301 cctcacgcgg agcatccgtt caccgcgcgt ccgaatatgc gatatcgcgt gatccggttc
    12361 ccgctgttcc gtcgccgttt ctgggagcag gtggctccgc tcctggtcgg cccgtacgat
    12421 gtgctccact tcccgtacga ttcctgcgtg gcctggaaac gcggaaagtt cgtggccacc
    12481 atccatgacg tcaagccgtt gctgtttccg gtgctccgtg cccgtaccaa tctcaatagt
    12541 cgcatcgaac agtggctggt cggggatcgc tggaagaagc tcgaccacgt cattacgatc
    12601 tccgaacatt cgcggcgcga tctcctggcg cacgttccgc ttcgccccga gcaggtgacc
    12661 gtgacctccc tgggcctcga tgccgagcgg tttcggcccg ccgagcatcc gggggagcgc
    12721 aagccctacg tgttctgtgt ggccggagcc gatccgacca agaacgtcgg cgtcctcatc
    12781 gacgcgttcg cgaaacttcc tgagatgctg cgcagccggt tcgacctaat cctggcgggt
    12841 gatgtctgca aacgccagga tattcgcgcg gccgtcgaga accatggcct cagcgcgcat
    12901 acgacactgg tcggcgtggt gtcggaccgg gaactcgtcg cctattatca gcaggccacg
    12961 ctgtttgtgt ttccttcgct ctacgagggg ttcgggttgc cggtgttgga ggccatgggt
    13021 tgcggctgtc cggtgatctg ttcgaacgcc tcttcgctgc ctgaagtggc cggcgatgcg
    13081 gcggtgctgt tcgatccgcg tgacggcggt cgattggccc ttgaactctc ccgtgtcctg
    13141 gcgtcacccg cgctgcggga cgaattgagg gcccgcggag tggctcgggc gcgcgaattc
    13201 tcctgggatc gcaccgcgat tgagacggtg gcggtctatc gacgtctggc aggctgagtc
    13261 agtgaccctc gatgggcgag ccgtatcggc ggcaacacgg atggtgctcg acaggttgca
    13321 gctcctgtgt tgtcatgggt ctcagaccgg ttcttgcccg atgtcgaggg gatgcacgat
    13381 atggcggaag agacgtgcga tgttttaatc gccggggccg gagtgatcgg cgtggccatc
    13441 gcgagggagc tcaggcgtcg atatggcgtc aaggtggtcg tgctggagaa ggaatccacg
    13501 gccggccggc atgcgagcgg tcgca
//
LOCUS       DAOQRM010000062        13493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3632_length_13493_cov_16.234261, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000062 DAOQRM010000000
VERSION     DAOQRM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13493
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3632_length_13493_cov_16.234261"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKK81_06755"
     CDS             complement(<1..188)
                     /locus_tag="PKK81_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32609.1"
                     /translation="MPSSSDSRASYHFLLGYQAELDQETELAIKEYQLALQTDPASNY
                     LKARLAVLYFTAGDVPSAV"
     gene            complement(448..1803)
                     /gene="dnaB"
                     /locus_tag="PKK81_06760"
     CDS             complement(448..1803)
                     /gene="dnaB"
                     /locus_tag="PKK81_06760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNV32610.1"
                     /translation="MKSLSAVDLTSPKLPPQNVEAEQSVLGAILLDNTAMAKVMELLT
                     DEEFYRTAHRKIYQAMLELSDRGEVIDQITLTECLKARSELEAVGGSAYLAELVQVVP
                     TAANVRYHSKIVRDKALLRGLIQTSTEVVTRGYDGTVAVDELLDFAERSVFSLAQGKL
                     DRSFTPVNQIIKESLDVVDKLSKRKERVTGVPTGFIDLDDLTAGLQPSDLIVIAGRPS
                     MGKTSLALGMAQHAALHAGTVVGIFSLEMSKPQLVLRMLSSEARVDSHALRTGRLQKE
                     DWWRLAEAAGKLEQAPIYIDDSGAVTVQQMRGKARRLKAERGLDLLIVDYLQLMQGKS
                     DSESRQQEISDISRSLKTLAKELNVPVIALSQLSRAVEARKPPVPMLADLRESGAIEQ
                     DADVVMFIYREEVYEPATERKGIAEILVSKHRNGPIGKRDLFFHDRFAKFENLESREV
                     V"
     gene            complement(1800..2924)
                     /gene="hisZ"
                     /locus_tag="PKK81_06765"
     CDS             complement(1800..2924)
                     /gene="hisZ"
                     /locus_tag="PKK81_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNV32611.1"
                     /translation="MGALSLKASSSSSPLVAGRERSLIPVGMSTILPDAARRIRQLEQ
                     TLLAVLARAGYEEIILPMFEYLDVLAPGLESELIEKCYQLVDRTTGRLMLLRPDATAQ
                     IARTVAMGMMGERLPLRLCYRTSVFRYEREHAGRDREIFQVGAELIGVDGVTGDAEVL
                     TLLLECLAQVGLSSFKVAVGHVGFFTALLVKAGLSQDGQKRVEQAAARKDMPLLEELL
                     TREGVSRSTGRVILEALELCGGPEVLVRGRKLVGRDRTLLRPLDRLAQVYERLVSPGQ
                     QAVLLDLGEFRGFEYYDGIVFDVFAPGIGAELGGGGRYDHLMGRFGRTAASTGFGLDV
                     DRLFRALDASGVVTPPHSSPSTTAGSKPVAAEPRRRRSRV"
     gene            complement(2943..4535)
                     /gene="serA"
                     /locus_tag="PKK81_06770"
     CDS             complement(2943..4535)
                     /gene="serA"
                     /locus_tag="PKK81_06770"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNV32612.1"
                     /translation="MKILVSDSLSKQGVEVLEKAGFTVVVKTKLPKEELLKEIKDADG
                     LIVRSGTKVTAEVIAAASQLKVVGRAGSGLDNVDTPAATRRGIVVMNTPGGNTVTTAE
                     HTMAMIFSMSRRIPQATASTKGGKWEKEKFMGVELYNKVLGIVGVGQIGGYLTKLAQG
                     VGMQVMAYDPYLAPERAEKMGVTIVELDELFRRADVISVHTPLTPETKALINAQAIAK
                     MKTGVMIANCARGGIVHEGDLCEALKSKKVAAAAFDVFEDEPVKPDNPLLALDNFICS
                     PHIGASTTEAQENVAIGIAEQIVEYFTKGIARGAVNIPSVSPELLPKLQPYLSLGERV
                     GLLQAQLLEGGLERLTVEYSGEVAGLNVAPLTIAVLKGLLTPILEDPVNYVNAPVVAK
                     ERGIEVKEVKISDAGDFASVIRVRVEAGKKSHQVAGTLYHRKDPRIIEIDQFKVEVVP
                     DNHLLLIQNEDRPGVIGTVGHILGDHNINIARMQCSREERGGKALQIFGLDAPLPKSV
                     LDQITNSKHILSVKVADLSKGL"
     gene            complement(4564..5709)
                     /locus_tag="PKK81_06775"
     CDS             complement(4564..5709)
                     /locus_tag="PKK81_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNV32613.1"
                     /translation="MLKRYLLAPGPTPVPPEVLLAMARPMIHHRAPEFDKLFAEVRED
                     LKWLFQTRNDVLILASSGTGGMEGSVSNFLSPGDKALTINGGKFGERWTKLCKTFGAQ
                     VTELKVEWGRAVDPQAVADALKKDPSIKAVYVQASETSTAVAHDVKALADIVKGYEET
                     ILVVDAITALGVLDLKTDAWGLDVVITGSQKALMLPPGLAFASVSEKAWRLADKAKNA
                     AFYFNFKRERENQQKSTTAYTPAVSLILGLKEVMNMLKAEGLEAVFARHAMLATAMRE
                     GVKAAGLSIFPQERPSDALTAISAPEGVDGQAVYKNLRTQYGMTAAGGQDHLKGKIFR
                     ISHMGYIDSFDVITALAAVEMVIKGLGYPIKLGSGVAKAQEIIMGKS"
     gene            6015..7406
                     /locus_tag="PKK81_06780"
     CDS             6015..7406
                     /locus_tag="PKK81_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNV32614.1"
                     /translation="MHARFLGTGVRALPVLSFCLVSLAWPALAGAESPTPSHADTPAT
                     TETTPEDIQPSIQEPEYSLDEEDHATAAELEAVEPPAPIASEPSTEVPPAEALLQLKP
                     LNGTTARFTDLLTPPAEVVQEEASAQDQESDEATAYNVPIVLDPSVQGHIRFFNVSIR
                     NRFEQWLIRLSHYRPLVDSIFSEFQLPSDLIYLSLVESGFNPHAYSRARAAGPWQFMK
                     GTAKVYGLRVDSYVDERRDPVKSTVAAARYLRDLYDLFGTWPLAMAAYNAGEGKVMRA
                     LHTAQAESFSDIAKTRLIRRETKEYVPRFMAATIIAKNPDRYGFPQNDVRPHQFEEVV
                     VKRPIHFKAIANVTGISYQELKVLNPELRRDATPPDDAEYHLKVPVGTREKVEQLLDR
                     APTHKFAPLPVPVRVRHVKPEPESGHWYRVRVGDSLERIAKRFNISVKTLKSNNNLTG
                     PTIKVGSRLVIAN"
     gene            complement(7447..8280)
                     /locus_tag="PKK81_06785"
     CDS             complement(7447..8280)
                     /locus_tag="PKK81_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32615.1"
                     /translation="MSYRIKVPAKVDPLDEAHLLTGVERFLLVLQEQRRAVLVGLGVL
                     LVAAAVVAGVIWYDYQATLKARELDQEATLHYLNRPADDPKKSHEQLAQAINLYKQVV
                     DQYPRSPVAPLALFHLGNAQVLANEVDAGIETYKRFMLLHGSNTSLLGLVQQRMAYAY
                     LVKGDRDQAVKAFTGILEIPGALNKDHVLFELAKLEESQSRPEGALAHYQDLMKNYPN
                     SPFTSEAAVRVKVLEVKKSPESPAAAATPAPAVTAPAPQPEAPAKSSGKSAAASSKKK
                     P"
     gene            8402..9562
                     /gene="bioF"
                     /locus_tag="PKK81_06790"
     CDS             8402..9562
                     /gene="bioF"
                     /locus_tag="PKK81_06790"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNV32616.1"
                     /translation="MFKEYLQKLQDQHLLRRLRTIASSTGPTVRLDGHTVILLSSNNY
                     LGLATHPAVVEAAVEATRQYGAGSGASRLVCGTLTPHEELETALARFNDTEATLTFAA
                     GYLANISAVPALIGKDGLILADRLCHASLIDGCRLSGATFRVYHHRDMNHLEQLLTRR
                     TSNKPTLIVTDGVFSMDGDIAPMRDIALLAERYGAAVYVDDAHGTGILGETGRGTLEH
                     CDVETRIPYHMGTLSKAIGSSGGYLTGSAEFVAYLVNTCRAFTYTTAPTPAAAAAATA
                     ALRVIRREPERRARLWRNRHRLAQGLSGLGFRLAASESPILPVIVGDPDRAMNLAHTL
                     LTLGVYAPAIRPPTVPASTSRIRFTITADHTDEHIDQALTALAQAGRTLNMI"
     gene            9741..10802
                     /locus_tag="PKK81_06795"
     CDS             9741..10802
                     /locus_tag="PKK81_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNV32617.1"
                     /translation="MIDISKLLTFGVQQGASDCHISAGEPPMIRLHGDLKKLDHPPLT
                     QDETHALIYDMMSDAQRKNFEEHRECDFSFDLGDIARFRVNVFVQGRGLGAVFRTIPT
                     EILPLEKLGMPPILRQLCDREKGLILVTGPTGSGKSTTLAGMIDYLNNTFEGHILTIE
                     DPVEFVHKSKKCLVNQRELGVHTLSFANALRAALREDPDIILVGEMRDLDTIQLALTA
                     AETGHLVFATLHTSSAPKTIDRIIDAFPPNQQAQVRAQLSETLEAVLTQTLLKKKSGG
                     RIAAVEIMVGTTAVRNLIREGKLHQIPGIMQASQKDGMQTMDMALVDLVTRGLVTKAE
                     AQSRSMNPNLFSAAAGGAA"
     gene            10859..12022
                     /locus_tag="PKK81_06800"
     CDS             10859..12022
                     /locus_tag="PKK81_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNV32618.1"
                     /translation="MDVRTLLEVMVKQESSDLYLTVDAPPIYRIHGSTHRTDAPPFTN
                     EQLESLALALMRGQQRGEFEEKMEMNLALYYKDLGRFRVNIFRQKGNVGLVFRHIKAE
                     IMTVEQLDLPPIVKDIAMTKRGLVLVVGATGSGKSTSLAAMIDHRNTVHAGHIISVED
                     PIEFVHHHKKSIVTQREVGFDTHSFGHALKNTLRQAPDVILIGEIRDTETMEAAITFA
                     ETGHLCLGTLHSNNANQSIERIMNFFPVERHAQIYLQLSLNLRAIISQRLIPSLDGRR
                     VPALEIMLDTPRIKDLIKRSEIDTLKEAMEQGIDEGCQTFDHVLLQLYKAGKISIEQA
                     LINADSANNLRLKIKLAGLKGDDAVAALLDKSDRDEKGFQIQGQLGQGGSKKR"
     gene            complement(12092..12448)
                     /locus_tag="PKK81_06805"
     CDS             complement(12092..12448)
                     /locus_tag="PKK81_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV32619.1"
                     /translation="MVPLVTGDEPIQELFVRAGCPVCHQIPGIAGAKGQVGPPLWLGK
                     TGASRLADPQYKGQAQTVREYIVESVVSPGTYVVPGFPPDTMPTWYGRKLSGAALSKI
                     ASYLEQAVEEPPSGRP"
     gene            complement(12662..>13493)
                     /locus_tag="PKK81_06810"
     CDS             complement(12662..>13493)
                     /locus_tag="PKK81_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNV32620.1"
                     /translation="VSVILFVGVWSVRAAEPLSGADGQAGGAEAITGQDGAPMVLVPA
                     GPFTMGSNDGLPAERPEHVVTLDAYVIDRYEVSLQLYRKFLQAARYDAPPTWDDEAAE
                     TVSDRPAVGMSWEDAAAYCAWAGKRLPTEAEWEKAARGTDGRRYPWGPMQPFVDIANY
                     NRGVWVSEAITLVSVAGGVEGMSVRHGLKAGGRSPYGLHHMAGNAAEWIADWYDRTYY
                     GKSPDKNPTGPANGEKRVIRGGSWADLPVALSASARVSAEPGFQDRTIGFRCAMDAKK
                     "
BASE COUNT         2907 a         4366 c         3582 g         2638 t
ORIGIN      
        1 accgccgacg gcacatcccc cgcggtgaag tagagcacgg ccagcctggc tttgagatag
       61 tttgaagcgg gatccgtctg tagggcaagt tgatattcct tgatcgccag ttccgtctcc
      121 tgatccaact cggcctggta tccgaggaga aagtggtagg acgcgcgaga atctgaggat
      181 gagggcatgg gacgaggagc caccggcacg gcggctggga gcgcagcagg agcgggacgg
      241 ccaggcggtg tgctgtggca ggcggcggtg acacaggcca tagccgcgag tagaagagct
      301 accgttctga cattcgcaga accccttcgg gcgggtggct gcgggcactc ttcacacaat
      361 cgttgcacaa tcatccgctc aacttgcagg gggaatattc gaaggacagt gactgacggg
      421 aattataccg gcccgagaaa gaacgcgtca aacgacctcg cgactctcga gattctcgaa
      481 cttggcaaac cggtcgtgaa agaataagtc ccgtttcccg atcggtccgt tacgatgctt
      541 actgaccaga atttcggcga tgccttttct ctccgtcgcc ggttcgtaga cttcttcacg
      601 atagataaac atcacgacat cggcgtcctg ctcgatcgca ccgctttccc gcaagtcggc
      661 caacatggga accggcggct tccgggcttc cactgcgcga ctcaactgtg aaagcgcgat
      721 gaccggcacg ttcagctctt tcgccagggt ttttaaggaa cgggaaatgt cggaaatttc
      781 ctgctgccgt gactcggagt cgctcttccc ttgcatgagc tggagatagt cgacaatcaa
      841 cagatcgagg ccacgctcag ccttaagccg cctcgctttc ccgcgcatct gctgcaccgt
      901 cacggcgccg gagtcgtcga tatagatcgg cgcctgctcc agcttcccgg ccgcttccgc
      961 gaggcgccac cagtcttcct tttgcaaccg accggtccgg agcgcatgcg aatcgacgcg
     1021 ggcctcggaa ctcaacatgc ggagcacgag ttgcggcttc gacatttcga ggctgaaaat
     1081 gccgacgacg gtgccggcat gcagcgcggc atgctgcgcc atcccaagcg ccagactggt
     1141 cttgcccata ctcggacggc cggcaatgac gatcaagtcc gacggctgta atccggcggt
     1201 caggtcatcc agatcgataa agccggtcgg tacgcccgtg acacgttcct tgcgcttgga
     1261 cagcttgtcg acgacatcga gactctcttt gatgatctga ttgaccggag taaaggaacg
     1321 atccagcttt ccctgagcaa ggctgaagac cgaacgctcg gcaaaatcca acagctcatc
     1381 gactgccacc gtcccgtcat acccgcgcgt cacgacctct gtcgaggtct gaatgagccc
     1441 tcgcaacagc gctttgtccc gaacgatctt gctgtgatag cggacattgg ccgccgtcgg
     1501 caccacctga acgagctcgg ctagataggc ggatcctccg accgcttcga gctcagagcg
     1561 cgccttcaag cattccgtga gcgtaatctg atcgatgact tcgccgcgat cggacagctc
     1621 cagcatggcc tgataaatct tgcgatgcgc agtccggtaa aactcctcgt ccgtcagcag
     1681 ctccatcacc ttggccatcg ccgtgttatc caacaagatc gcgccgagca ccgactgttc
     1741 ggcctcgacg ttctgtggcg gcaatttggg ggaggtgaga tcaaccgcgg agagcgactt
     1801 catacccggc tcctgcgccg tcgcggttct gccgcaaccg gcttggagcc agcggttgta
     1861 gagggactgg agtgaggcgg cgtcaccaca ccggaagcat cgagggctcg aaacagacga
     1921 tcgacatcga gcccaaaccc ggtagaagcc gcggtccgac cgaaccggcc catcaaatgg
     1981 tcgtatcggc ccccgcctcc cagctccgcc ccgatacccg gcgcaaagac gtcgaagact
     2041 atcccgtcat aatattcaaa cccgcggaac tcgcccaaat cgagcaacac cgcctgctgc
     2101 ccgggcgaca ccaatcgctc atagacctgc gcaaggcgat cgagtggtct caatagggtc
     2161 cggtcgcgcc ccaccaactt tctcccgcgc accaagacct caggcccacc gcacagttcc
     2221 agcgcttcaa gaatgacccg ccccgtcgat cgggacacgc cctcccgcgt cagcagttcc
     2281 tcaagcagcg gcatgtcttt ccgcgcggca gcctgctcca cacgcttctg gccgtcctgc
     2341 gataagccgg ccttgaccag cagggccgta aaaaatccga cgtggccgac agcaaccttg
     2401 aatgaagaca acccgacctg tgccaaacat tccagtagga gcgtcaacac ctcggcatct
     2461 cccgtaaccc cgtccacgcc gatgagttcc gctccgactt gaaaaatctc gcggtcgcgg
     2521 cccgcatgct cacgctcata ccggaacaca gaggtgcgat aacacaaccg cagcggaagc
     2581 cgctcgccca tcatacccat ggcgaccgtt ctggcaatct gtgccgtggc atccggacgc
     2641 agcagcatca accggcccgt cgtccgatcg accagctgat aacatttctc gataagttcg
     2701 gactccaggc ctggcgcgag gacgtccaga tactcgaaca tcggcaggat gatttcttcg
     2761 taacccgcgc gagcgagcac ggccagcagc gtctgctcga gctgtcggat acgtcgagcg
     2821 gcgtcaggaa gaatggtgct cattccaacg ggaatgagcg accgttcccg acctgccacc
     2881 agaggcgagg aggaagagga agccttcaac gaaagagcgc ccatggtcga ctgccgggca
     2941 atctacaacc ctttcgacag gtcggcgacc ttcacggaaa ggatgtgctt gctgttcgta
     3001 atctgatcga ggactgactt aggaagcggc gcatcgagcc cgaaaatttg cagggctttg
     3061 ccgccacgct cttcccgtga acactgcatg cgcgcaatat tgatgttgtg atcgccaagg
     3121 atatgcccca ccgtgccgat cacccccgga cgatcctcgt tctggatcag caggagatgg
     3181 ttgtccggca ccacttccac cttgaactga tcgatctcaa tgatccgcgg gtccttgcga
     3241 tggtagaggg tcccggcgac ttgatgagat ttcttcccgg cctcaacccg cacacggatc
     3301 acactggcaa agtctccggc atcgctgatc ttcacctctt tgacttcgat gccacgttcc
     3361 ttggcgacga ccggcgcatt cacatagttg accgggtctt caagaatggg ggtcaggagt
     3421 cccttcagaa ccgcgatcgt caatggcgcc acgttcaaac ccgccacttc tccgctatac
     3481 tcgacggtca accgctccag cccgccttcc aacaattgcg cctgcaagag gccgacacgc
     3541 tccccaagcg aaagatacgg ctggagtttc ggtaagagct ccggtgagac cgagggaata
     3601 ttgaccgccc cccgggcaat ccctttggtg aagtattcca cgatctgctc ggcaatgccg
     3661 atcgccacat tttcctgcgc ttccgtagtg gaggcgccga tgtgcggcga gcagataaaa
     3721 ttatccaacg ccaggagcgg attgtccggc ttgaccggct cgtcttcgaa cacgtcgaat
     3781 gccgcggcgg caaccttctt ggatttgagc gcctcgcaca aatctccctc gtgcacgatt
     3841 ccaccacgcg cacagttggc aatcatgacg ccggttttca tcttggcaat ggcttgcgca
     3901 ttgatcaggg ctttggtttc cggggtcagc ggagtatgca cggaaatgac atcggctcgg
     3961 cggaacagct cgtccaactc gacgatggtc acacccattt tttccgcacg ctccggggcc
     4021 agatagggat cgtaggccat cacctgcatc cccactccct gtgcaagctt ggtgagatac
     4081 ccgccgattt ggccgacgcc gacaatgccg agcactttat tgtacaactc cacgcccatg
     4141 aacttttctt tttcccactt cccacccttt gtcgaggcgg tagcttgcgg gatgcggcgc
     4201 gacatcgaga aaatcatggc catcgtatgc tcggcagtcg tcacggtgtt gccaccgggt
     4261 gtattcatga cgacgatgcc gcggcgcgtg gcggccgggg tatcgacatt gtccaagccg
     4321 gaaccggctc gaccgacgac cttcagctgg ctggcggccg cgatgacttc tgcggtgact
     4381 ttcgtcccgg agcggacgat cagtccgtcc gcgtccttga tttccttcag cagctcttct
     4441 ttcggcagtt tggttttcac caccaccgta aagccggcct tctccaatac ctcgacaccc
     4501 tgcttcgaga gactatcgct gaccaaaatt ttcattgtcg tgacgcctta cctgacggtg
     4561 agtctaagac ttgcccataa taatttcttg agcctttgcc accccgctac cgagttttat
     4621 gggatagccc agccccttga tgaccatctc caccgccgcc agggccgtga tgacgtcaaa
     4681 actatcgata taccccatgt gagaaatacg gaagattttg cccttcaggt gatcctggcc
     4741 acccgccgcc gtcattccat attgggtccg caaattctta tagacggcct gtccgtccac
     4801 accttcaggc gccgaaatcg cggttaacgc gtcgctcgga cgctcttgag ggaagatgga
     4861 gagccctgcg gccttgacgc cttctcgcat cgccgtcgcc aacatcgcgt gacgggcaaa
     4921 gacggcttcc agcccctcgg ctttcaacat gttcattact tctttgaggc ccagaatcag
     4981 ggacacagcc ggcgtatagg ccgtcgtgct cttctgctga ttctcacgct cgcgcttaaa
     5041 attgaaatag aacgccgcgt tcttcgcttt gtcggccaat cgccaggcct tttcactcac
     5101 gctggcaaag gctaagcccg gcggcaacat taaggctttc tgcgacccgg tgatgactac
     5161 gtcgaggccc caggcatcgg tcttgagatc gaggactccc agggcggtaa tcgcatccac
     5221 gaccaaaatc gtctcctcgt accctttgac gatatcggcc aaggctttga cgtcatgcgc
     5281 cacagccgtg gaggtctcgc tcgcctgcac atagacggcc ttgatcgacg gatccttctt
     5341 caaggcatcg gcgacggcct gcggatccac cgcacggccc cactcgacct ttaactcagt
     5401 gacctgcgct ccgaacgtct tacacagctt cgtccaacgc tcgccgaact tgccgccgtt
     5461 gatcgtgagg gccttatccc ccggggacaa gaaattcgaa accgagccct ccatccctcc
     5521 ggtgccggaa gacgcgagaa tcaacacatc gttcctggtc tgaaacagcc acttcaggtc
     5581 ctcacggacc tcggcaaaca gtttatcgaa ttcgggcgcc cggtgatgaa tcatgggtct
     5641 ggccatggcc agtaacacct ccggaggaac gggggtggga ccgggagcta gcaaataccg
     5701 tttgagcatg ggacgaacct cctcactgtg cagatcgaac gatgtcaggg atttagaagg
     5761 ggcgtacgct accatagccc ctctgaggtg tcaaggtaac gaaccgtgaa gatgcttatc
     5821 ttttcagcga cttcactgca tcgcgcaaga tttctttcag agacgcagcg cgatctcctg
     5881 cgcactgttt tctccctgta aacgcgatga cgactcaact ccctcaattt cttgacaagt
     5941 tgtaccctcc tcgataggct acccttcgag agtcagcatt ctcacatttc ctgaacagtc
     6001 tgatgggtac ccttatgcat gcacgtttcc tgggcacagg tgttcgcgct ctgccggttc
     6061 tctcgttctg cctggtctct ctcgcgtggc cggccctcgc aggagcggag tcgcccaccc
     6121 catcccacgc cgacacaccc gcgactacgg agactacgcc ggaagacatt caaccatcca
     6181 ttcaggaacc ggaatactcg ttggacgaag aagaccacgc cactgccgcg gaactcgaag
     6241 ccgttgagcc cccggccccc attgccagcg aaccgtcgac cgaagtcccg ccggccgaag
     6301 cgctccttca actcaagccc ctcaatggca ccaccgctcg attcacggat ctgctgacac
     6361 cacccgcaga ggtggttcag gaagaggctt ctgcgcagga ccaggaatcg gatgaagcca
     6421 cggcctacaa cgtgccgatt gtgctcgacc catccgttca aggccatatt cgttttttca
     6481 acgtctctat ccgcaatcga tttgaacagt ggctcatccg cctgagtcac tatcgcccgc
     6541 tggtcgacag catcttctct gaattccagc tccccagcga tctcatctat ctgtctctgg
     6601 ttgaaagcgg atttaacccc cacgcttatt cccgcgcccg tgcggccggt ccatggcaat
     6661 ttatgaaagg gaccgccaaa gtctatgggc tgcgtgtgga tagttatgtg gatgaacggc
     6721 gagaccctgt gaagtcgacc gtcgcggctg cgcgctacct gcgggacctg tacgatttgt
     6781 tcggcacctg gcctttggcc atggccgcct acaacgccgg tgagggcaag gtgatgcgag
     6841 ccctccatac ggcgcaagcg gagagctttt cagacattgc aaaaacacgg ctgatccggc
     6901 gggaaacgaa agaatacgtc ccccgattca tggccgcaac catcatcgcc aagaatccgg
     6961 accgctatgg cttcccgcag aacgacgtgc ggcctcacca gtttgaagaa gtggtcgtga
     7021 agcgccccat ccactttaaa gcgattgcca atgtgacggg catttcgtac caggaactga
     7081 aggtactgaa cccggagtta cggcgcgatg ccacgccccc ggacgatgcc gaatatcacc
     7141 tgaaggttcc ggtcggcacc agggagaagg tcgaacaatt gttggatcga gcgccgactc
     7201 acaaattcgc gccgctcccg gtccccgtca gggtccggca cgtcaagcct gagccggaat
     7261 ccggccactg gtatcgggtg cgcgtaggcg actctctaga gagaatcgcc aagcgattca
     7321 acatctccgt caaaaccctc aagtccaaca ataacctgac aggtcccacc atcaaggtag
     7381 gaagccgcct ggtcatcgcg aactaacgcg accacccctc accgcgtcat tctactaagg
     7441 gagccgttac ggttttttct tggaggacgc cgcggcagac ttgcctgaag acttagcagg
     7501 cgcctcaggc tggggagcgg gcgcagtcac ggccggagca ggtgtagcag ccgccgccgg
     7561 actctccgga gacttcttca cttccagcac cttcactcgc acggccgctt cactggtgaa
     7621 gggcgagtta ggataattct tcatcaaatc ctggtagtgc gcgagggctc cctcaggacg
     7681 ggactgcgat tcttccaatt ttgccagctc gaagagcacg tggtctttat tcagcgcacc
     7741 ggggatctca aggatgccgg taaacgcctt caccgcctga tcacgatccc ccttcaccag
     7801 ataggcgtag gccatccgct gctggacaag ccccaggagc gaggtattgg aaccatgaag
     7861 caacatgaat cgtttgtagg tttcgatccc tgcatcaacc tcattagcca gcacctgagc
     7921 attccctaaa tggaacaggg ccaggggagc aacggggctc cgtggatatt gatccactac
     7981 ctgcttgtag agattgatgg cctgcgcaag ctgttcgtga gattttttcg gatcgtcagc
     8041 cggtcgattg agatagtgca gcgtcgcttc ctgatcgagt tcacgtgctt tcagcgtggc
     8101 ctggtagtca taccaaatga ccccggcaac caccgccgcg gcaacgagca agactcccag
     8161 cccaacgaga actgcccgcc gctgttcctg caagacaagc agaaaccgct ccactccggt
     8221 cagcagatga gcttcatcga gcggatcgac ctttgcggga accttgattc gataactcat
     8281 gctctctcaa tcattctcta ggcgcagtaa aagaagcggt cgtttccgag cgcctcgcgc
     8341 cttatactag ggagcagtca gccttgtcaa atattcgagc ccttcgcgag tacgggacac
     8401 catgttcaag gaatatcttc aaaaattgca ggaccagcac ctactccgtc ggctgcgcac
     8461 catcgcctcc tccacggggc cgacggtcag gctcgacggg cacaccgtga tcctgctttc
     8521 ctccaataat tacctgggcc tggccaccca cccagccgtc gtagaagcgg ccgtcgaagc
     8581 gacgcgtcaa tacggggcgg gatccggggc ctcgcgtctc gtctgcggca cccttacgcc
     8641 ccacgaagaa ctcgaaacgg cactcgcccg gttcaatgac actgaagcca cactcacctt
     8701 cgcagccggc tatctggcga atatcagcgc cgttccggcg ctgattggaa aagacgggct
     8761 catcctcgcc gaccgcctct gccacgcaag tctgatcgac ggctgccgct taagcggcgc
     8821 cacatttcgc gtctaccacc accgggacat gaatcacctg gagcaactcc ttacccgccg
     8881 gacctccaac aaacccaccc tgatcgtgac cgacggcgtc ttcagcatgg atggggacat
     8941 cgcaccgatg agggacatcg ccctattggc ggaacggtat ggcgccgccg tctatgtcga
     9001 cgatgcgcac ggcacgggaa tccttgggga aacagggcga ggcaccctcg aacattgcga
     9061 tgtcgaaacg cgcatcccct atcacatggg caccttgagc aaagcgatcg ggagctccgg
     9121 aggttacctg actggatcag cggaattcgt ggcctatctc gtaaatacct gccgcgcctt
     9181 cacctataca accgccccca cgccggccgc cgccgcggcc gccacggcgg cgctccgcgt
     9241 gatccggcga gagcctgagc ggcgagccag attgtggcgg aatcgccatc gccttgcgca
     9301 aggactcagc ggcctcggct tccggctcgc cgcttcggag agccccattc ttccggtcat
     9361 cgtcggcgat cccgatcgcg caatgaacct cgcccacaca ctgctcacac tgggagtcta
     9421 tgcccccgcc attcgcccac cgacggtgcc ggcgtccacg agccggattc gcttcacgat
     9481 caccgccgat cacaccgatg agcacatcga ccaggcgctg acggcactcg cacaggcagg
     9541 tcgcacgctc aacatgatct aagcaccaat cgccctttgc agagcggctg ccagccaccg
     9601 tttctccctc gtctcttctg ggtattttct tgctttctca ccttcctatg gcatgatcga
     9661 atcaacctga cgagccatga tcctctactc atagcgacgc tcgtttatcc atacattctc
     9721 tcactgcatg gagtgatccg atgattgata tctccaaact gttgaccttt ggcgtccaac
     9781 agggcgcctc ggactgccac atcagcgccg gtgaaccacc gatgatccgc ctgcatggcg
     9841 atctcaagaa actcgaccat cctcccctga cccaagatga aactcacgcg cttatctacg
     9901 acatgatgag tgacgcgcaa cggaagaatt tcgaagaaca ccgggagtgt gacttctcct
     9961 tcgacctggg cgacatcgcc cgattccgcg tcaacgtctt tgtgcaaggg cgcggcttag
    10021 gcgccgtgtt ccggaccatt ccgacggaga ttcttcccct ggaaaaactc ggcatgccgc
    10081 cgatcctccg acagctctgc gaccgcgaaa aaggcctgat cctggtgacc ggcccaaccg
    10141 ggtccgggaa atccaccaca ctcgcgggca tgatcgacta tctcaacaac accttcgaag
    10201 gacacattct cacgatcgaa gatccggtcg agtttgttca caaatccaaa aaatgcctgg
    10261 tcaaccagcg ggaactgggc gtccacacgc tgtcgttcgc caacgccctc cgcgccgcac
    10321 tccgcgaaga ccccgacatc attctcgtcg gcgaaatgcg agacctggat acgatccagc
    10381 tggcgttgac cgctgccgaa accgggcact tggtctttgc gaccctgcac acgtccagcg
    10441 ccccgaaaac catcgaccgc atcatcgacg ccttccctcc gaaccaacag gcccaagtcc
    10501 gcgcacagct ctcggaaacg ctggaggcag tcttgaccca gacgctcctg aagaagaaga
    10561 gtggtggccg gattgcggcg gtggaaatca tggtcggtac cacagcggtg cggaacctca
    10621 tccgcgaagg caaactccat caaattcccg gcatcatgca ggccagccaa aaagatggga
    10681 tgcaaaccat ggacatggcc ctggtcgatc tggtcacacg agggctggtg acaaaagctg
    10741 aagctcaatc gcgcagcatg aatcccaatc tctttagcgc cgctgcagga ggcgccgcgt
    10801 aaacgggcac tcggcgcaaa cgaaacgggt ttcccacggc attggaaaga ggcatttcat
    10861 ggatgttcgc acgcttctcg aagtgatggt caagcaggaa tcgtcggacc tgtatctcac
    10921 cgtcgatgcg ccccccattt atcgcatcca tggctcgaca catcggaccg atgcgccgcc
    10981 gttcacgaac gagcaactcg aatcgctggc gctggccctc atgcgaggcc agcagcgagg
    11041 tgagttcgaa gaaaaaatgg agatgaacct tgccctttat tacaaagacc tcggccgctt
    11101 tcgcgtcaac atcttccgac agaagggcaa cgtcgggttg gtgtttcgtc atatcaaagc
    11161 ggagatcatg acagtcgaac agctagacct gccgcccatc gtcaaagaca tcgctatgac
    11221 caagcggggt ctggtgttgg tggtcggcgc gaccggctcc ggaaaatcca cgtccctcgc
    11281 cgcgatgatc gaccaccgca acacggtgca tgcgggccac atcatcagcg tggaagaccc
    11341 gatcgaattt gtccatcacc acaagaaatc gatcgtcacc cagcgcgaag tcggcttcga
    11401 cacccattcc ttcgggcacg cgttgaaaaa taccctgcgt caggcaccgg acgtcattct
    11461 catcggtgaa atccgggata cggaaaccat ggaagccgcg atcacgttcg ctgaaaccgg
    11521 acacctctgc ctcggaacgt tgcactcgaa taatgcgaat caatcgatcg agcgcatcat
    11581 gaacttcttc ccggtcgaac ggcacgcgca gatctatctg cagctctcat tgaacctgcg
    11641 ggccatcatc tctcaacgat tgatcccctc cctcgacggc cgccgcgtgc ctgcactcga
    11701 aatcatgctg gatacaccgc gcatcaagga cctcatcaaa cgttctgaga tcgataccct
    11761 gaaagaggcg atggaacagg gcatcgacga aggctgccag acattcgacc acgtgctgtt
    11821 gcaactctat aaggccggga agatcagcat tgaacaagcc ttgatcaacg ccgacagcgc
    11881 caacaacctc cgtctgaaaa ttaaactggc ggggttgaaa ggcgatgacg cagtggccgc
    11941 cctcctggac aagagcgacc gggatgagaa ggggttccag attcaaggac aactgggtca
    12001 gggcgggagc aaaaagcgct gagggtcagg gtattgctgc tccctctatt cctccgcgag
    12061 cgcacaccac gcaattgatc ggcacacccg cttaagggcg tcccgagggg ggctcttcca
    12121 ccgcctgttc gagatacgac gcgattttac tcaatgcagc cccactcagc ttccgtccgt
    12181 accacgtcgg catcgtatcc ggtggaaacc ctggcaccac ataggtcccc ggggatacca
    12241 ctgattccac aatgtactca cgaaccgtct gcgcctgccc tttatactgt ggatcagcca
    12301 ggcgactcgc cccggtcttc cccagccaca gtggtggacc gacctgcccc ttcgctcctg
    12361 caatgccggg aatctgatgg caaaccggac agccggcgcg gacaaagagt tcctgaatcg
    12421 gttcatcccc cgtcaccaac ggcaccatgt tcgggtcaat attctggaca gccggggttg
    12481 aatcagatgg aggcgagata ttgcggatcc ccgccagcgc aaccaggaac acaagccctg
    12541 ccgcgaacgc ggcaacggcc ttgtgctttc ggttcggcgg tggaacagac gatgcggcac
    12601 ctgaatcaag cataggcagg aaccaggaaa cttacgaagg cctcagtgct cacccctgcc
    12661 tctatttctt ggcatccatc gcgcagcgaa atccaatggt gcggtcctga aaaccaggct
    12721 ccgccgagac ccgcgcagac gcacttaacg ccacaggcaa atcggcccac gacccgccac
    12781 gaatcacccg tttctcgcca ttagcaggac ccgtcggatt tttgtcggga ctcttgccgt
    12841 aatacgtacg atcgtaccaa tcggctatcc actcggccgc attccccgcc atgtgatgga
    12901 ggccgtacgg actccgcccc ccggctttca gcccgtggcg gacactcatc ccttccaccc
    12961 caccggccac actcaccaac gtaatggctt cgctcaccca cacgccgcga ttgtagttgg
    13021 caatgtccac gaacggctgc atggggcccc acgggtaccg acggccatcg gttccacgcg
    13081 cagccttctc ccattcagct tccgtcggga gtcgcttccc tgcccaggca caataggccg
    13141 ccgcatcctc ccaactcatc ccgacagccg gacgatcgct gaccgtctcc gccgcttcat
    13201 catcccaggt cggcggcgca tcatatctcg ccgcctgaag aaactttcga tagagttgca
    13261 gcgacacttc atatcgatcg atgacatacg cgtcgagggt caccacatgt tcggggcgct
    13321 cggcgggaag cccatcgttg ctccccatcg taaaaggccc ggccggaacc aacaccatcg
    13381 gcgcaccatc ctgccctgtg atcgcctcag ccccgcccgc ctggccatcc gccccagaca
    13441 gtggctcggc cgctcgcacc gaccagactc cgacaaacag aatgacggat acc
//
LOCUS       DAOQRM010000063        13297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3717_length_13297_cov_15.499924, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000063 DAOQRM010000000
VERSION     DAOQRM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13297
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3717_length_13297_cov_15.499924"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..1774
                     /locus_tag="PKK81_06815"
     CDS             281..1774
                     /locus_tag="PKK81_06815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNV32621.1"
                     /translation="MDADESTGPTSQTPAAPLPCVGAPPPPHAYDPGFSRAITWLSAV
                     VLSASIAVVAWLSFDVPRVERVPDAERALSHMVGRLMDQEDGLKTLPVWEQFLYEATM
                     GSDANDREQAIEWYRELAEESSDPSVDLHLAILEAESGYLPAVQQKMARWVRQGEPYP
                     TFARLLQAGYVDPRVPPASGFELQAYLAEQSVSGWFYSRLATRIAERAGDRPLLVTIE
                     TSLQQRVEALLWRSRAFALLELTLMIVGLLVLVLWVRRGQGTAMFRVGSAELPPLWAG
                     RLGAGVLLRGGAVGALLTVAFLYVAGDYPSLRVVAVPLSNLPLLALAYYHLLRPQRQT
                     FWRGFGLRIEPRHLGQLGLAVLAVVAAGLVGEWVLGRIAEPLNLISHWTEWFDADLVW
                     GSSPTLMVSLMEYVFFAPVFEELAFRGLLFGVFRRRFQWGVAAMLSAALFALAHGYGL
                     IGFLSVFWSGVIWAWAYERTGSLWPGMIGHAINNLLVCLSVMALLRA"
     gene            complement(1794..>1979)
                     /locus_tag="PKK81_06820"
     CDS             complement(1794..>1979)
                     /locus_tag="PKK81_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32622.1"
                     /translation="ICAEVSGRWGVVSARQADRSESGKRIRNHKILTHVGYTLRHPEF
                     YDALQSIEADRRPPIRL"
     assembly_gap    1980..2079
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2080..2409)
                     /locus_tag="PKK81_06825"
     CDS             complement(<2080..2409)
                     /locus_tag="PKK81_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32623.1"
                     /translation="MTTPRSLVILGSGYTGRWIYKLAVQQSLPILASSRHPDRHLSDV
                     EPSRRIRFDLAEPSTWSGLPPDADLIWTFPATPLEQVQAFARHSCRPPGRLVVLGSTS
                     AYDREGGP"
     gene            2511..3653
                     /locus_tag="PKK81_06830"
     CDS             2511..3653
                     /locus_tag="PKK81_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNV32624.1"
                     /translation="MGPFSARQEETRESSRQSADDPSHRRQRLMVGAAGLLACLFFIL
                     DLQLPLGVANAVLYSAVVFLSAAGQYRWLPIGTAITCSLLTIMAAPFSPRVPGLPAWF
                     EWGNHLFSLFGIWTPVMFIYQRRRTELLLKEVNERLEQGVEARTADLAASRLALERSE
                     EQLHTLTGRILTAQEEERRRIAQDLHDDVNQRLALLMLDLQAVDRQIGSAPAEAQDGI
                     RNALKGLEELSDDVRFMAYRFHPSILDDLGLKAALQRLLDDFSSRTGVKALFVHQPLD
                     HQLDKTTSTAVYRVIQESLSNIARHAKATRVEVEVTVEDEGLVVVVRDDGRGFDQRAI
                     DRAEGGLGLLNMRERLLAVQGSCEVESNPGRGTTVSMYVPLVRVAT"
     gene            3650..>3924
                     /locus_tag="PKK81_06835"
     CDS             3650..>3924
                     /locus_tag="PKK81_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV32625.1"
                     /translation="MTLPRVLLADDHTLVLEGFRRLLDDQCELVGTVGDGRALLEAIP
                     ELKPDIVILDISMPVLNGIDAARVLKVKFPQVKVVFITMHADPAYVRA"
     assembly_gap    3925..4024
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4025..4448
                     /locus_tag="PKK81_06840"
     CDS             <4025..4448
                     /locus_tag="PKK81_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV32626.1"
                     /translation="KMSRPRVLIGDSSRAMLAFLEILLEPQFEVVSSETEAEAIVLTA
                     KKIAPELVILDFSLSFLEGLGAARQLYETMPNSKVVFFSSHEDPVYVTAAFEAGARGY
                     LVKHLTVDLGHYLGRVLQGERVCCPDGLHTRVARTKDT"
     gene            4498..4698
                     /locus_tag="PKK81_06845"
     CDS             4498..4698
                     /locus_tag="PKK81_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32627.1"
                     /translation="MVRVVPMCELCRRVRDEGASTWGTNSWVDFPSYLARHVVPPSQV
                     RFSRDYCSECRLSYDILKTYGG"
     gene            4917..>8260
                     /locus_tag="PKK81_06850"
     CDS             4917..>8260
                     /locus_tag="PKK81_06850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV32628.1"
                     /translation="MSALPFHPIVAEWFTTRFASATNVQMQAWPAIQSGRDVLISAPT
                     GSGKTLAAFLSCIDTLFQQAVRCELRDETQVLYVSPLKALSNDVQKNLQQPLSEIGQA
                     ALASGMLLPELRVVVRTGDTPMTERQQMLRRPPHILITTPESLFILLTAEKSRAMLKT
                     VRTVIVDEIHAVAPNKRGAHLALSLERVAALTGVEVQRIGLSATQRPIETIAKFLVGN
                     RNPSIVNGHTSCEAVSRKPLAISSAPCTIIDVGHRRDMDLAVEVPKDELGAVATNAIW
                     SDIYDRVAALVEAHRSTLVFVNTRRLAERVSHYLEERLRHLGDEVVAAHHGSLSRTIR
                     LSAEDRLKTGAVRVVVATASLELGIDVGTVDLVCQIGSPRSIATGLQRIGRAGHWIHA
                     IPKGRLFATTRDELIECAALIRAIRAGVLDRIEVPPAPLDVLAQQIVAAAATQSWDEA
                     ELYDLCRRAMPYRDLDRSTFDAVVTMLADGYVTSRGRGRAYLHHDRINHHIRGRRGAR
                     LAAITSGGAIPDTANYAVIAEPDGTVVGSVDEDFAVESLAGDIILLGNTSWRIKGVET
                     GKMRVEDAQGAPPTIPFWRGEAPARTAELSAEVARLKADIDHRLTTDQALSMASAPPV
                     QWLRQECGLDQRGAQQAVEYILAGKAVLGTVPTQQTIVAERFFDESGGMQLVIHAPFG
                     GRINRAWGLALRKRFCVTFDFELQAAATDEGIVLSLGEKHSFPLETVFAFLNVNTLRE
                     VLTQAVLQAPMFMTRWRWNASRALALLRFVGGKRVPPQIQRMRAEDLLAAVFPDAIAC
                     QDNFQGARTIRQIPDHPLAQETIRDCLTEAMDLDGLIAVLEKIERGAIACLAVDTPMP
                     SAFCHEILNANPYAFLDDAPLEERRARAVDMRRSLPPELAGGMGALDQSAIDQVSEES
                     WPVVRDAEEFHDALLSLGWVPCERMPGWDLLVPKLAAAGRVATLWQGETKLGWLAGEY
                     RHYAGLLFPDARIDPATGPIDPTEQVEQEEVLNRVVLGWMESIGPTTAGELSQTLHLS
                     ESDVQSTFLRLESQGHLLRGQFRQTLSARSETKDPSSHLSPFTSHEFCHRRLLARIHR
                     LTIGRLRREIEPVTAAEFMRFLFQWQ"
     assembly_gap    8261..8360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8361..9224
                     /locus_tag="PKK81_06855"
     CDS             <8361..9224
                     /locus_tag="PKK81_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV32629.1"
                     /translation="RRVIPTRVAPVGIFAREDGPVLLAAAGEELARLDLSARLSGSAQ
                     AIRGCLQARGASFFSELLHGTRLLASEVENGLWELVAAGLVTADGFDNLRSLIDPKRR
                     RAEASDRSRRPRHVGGRWSLLRPMENHQPSSGSSQQSNSAPATEHLARQLLQRYGVVF
                     RDLLGRESMVSSWRDLLVCYRRLELMGEIRGGRFVSGFTGEQFALPGALEALRALKKR
                     PGAATQQDIKISAADPLNLAGLILPGPRIAAVPSNFVVFRDGMVVRTVTGRETNDRQV
                     SPVVEVGRVGG"
     gene            complement(9254..10108)
                     /locus_tag="PKK81_06860"
     CDS             complement(9254..10108)
                     /locus_tag="PKK81_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV32630.1"
                     /translation="MNALHPPFTIHRIFHPTDFSQDSQVAFAHALKLALVFRAELTIM
                     HVDPAVSPEGFEDFPRIRPTLAQWGLLPESSSKGDVTKLGIRIRKVRALASDAKQAII
                     HHLTASPTDLMVLATHQHEGFARWVHHALAEPVSREMQVKTLFVPSHVEGFVSRETGQ
                     TSLQRLLLPISIQPPSQPAIDAASALISELSAPPVTLTLVHAGKEPDVNALELPQKAG
                     WSWNQLFGKGDPVEWILAAGAEFDVDLIVMATKAQDSLLDMLRGSTTERVLRGARCPL
                     LAIPAKLI"
     gene            complement(10177..10572)
                     /locus_tag="PKK81_06865"
     CDS             complement(10177..10572)
                     /locus_tag="PKK81_06865"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNV32631.1"
                     /translation="MIANHRPIVAAFGGLSLVLLLSACSFKATFKETTDTTSNITGTT
                     SGRIWWNEDGLLKPEHKVAAFTAYNADNLQSDAARGQGEYLASLDRLMGASDASVFQP
                     FAQDSFSRWSRTGSTSTTDLVNDLQSALR"
     gene            10826..11266
                     /locus_tag="PKK81_06870"
     CDS             10826..11266
                     /locus_tag="PKK81_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HNV32632.1"
                     /translation="MKTRVLLYGLVGVTLGVAPVRAESVPMKPGEYQVTAVTDASNGE
                     AGKPDTRTRCVREEHLANPDAVFNYYALNGFQPNPSSKVVNVSMHGGKVSYDIEGVYA
                     VTRVEGTVSPTGFSVVRKATPKGDHALSVTMKVDAKRTGDCPGK"
     gene            complement(11355..>13297)
                     /locus_tag="PKK81_06875"
     CDS             complement(11355..>13297)
                     /locus_tag="PKK81_06875"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HNV32633.1"
                     /translation="HLFNPTNLALAIALASGMGWISPGQWGQVAWFGFLIACLGSLVV
                     TRAARADVTFAFLAVYVGLLFARALWLGDPLTIPFHQIESGALLIFAFFMISDPKTTP
                     DSRAGRIVYALCVALAALYVQFGLFRPNGPLWGLIACTPLVPLLDHLFPGARYVWTRP
                     STGSASPNSSLHINAQPSAGSHPQSVSVSLVSPTKEVLMRRTIVPSLLFALGLLAWSG
                     EASAFCGFYVGKADTRLFNKASEVAIARHDNKTVITMANDFKGDVKEFALVVPVPTIL
                     EKEQIHVGDPAVLKHLADYSAPRLVEYFDENPCLRYELMERRSMDAMKSMAPAAAPAR
                     ERDKALGVTVEAQYTVGEYDILILSAKESNGLETWLVENGYRIPNGASAVLHSYLKQG
                     MKFFVAKVNLGEQTKLGLTHLRPLQIAFESRKFMLPIRLGTVNADGAQELFIYFLTKQ
                     GRVETTNYRTVRLPEAQEIPLYVKDKFGDFYRDLFTQQVKRESERGVFMEYAWDMNWC
                     DPCAANPLSADELRSLGVFWQDGHGRMGKGIPMAQNVFLTRLHVRYDAAHFPEDLMFQ
                     ETSGHSNFQARYILRHPWTGTDECAAATAYRHQLRERYEREAQTLASLTGWNIGDIRK
                     TMNIASVPAGEEKKWYQRLWTN"
BASE COUNT         2270 a         3659 c         4339 g         2729 t
ORIGIN      
        1 gtccgatcct ctcgcccggc ggggctgttc tcgttcggcc tcctcgacgg actgcgagta
       61 cgcctccgtc ggcctcgctt gcgagaagcc ccggctggct tcggtctcga acgcctcgcg
      121 acggcattca tgaatcgtcc gggctagtat aacgaaaccg gcggggattc agccatggga
      181 ttgtccgcag ctcccgcctg cgatctacac tgcgttgcgc cagtttcacc tcttgaaagc
      241 cggtggtata ctccggcgcc gatcacgccc cggaggacct gtggacgcag acgaatcaac
      301 cggaccgaca tcgcagacgc ccgcggctcc gttgccctgc gtcggggcgc ctcctccccc
      361 gcatgcttac gatcccggct tttcccgtgc catcacctgg ttgtcggcgg tcgttctcag
      421 tgcgtcaatt gcggtggtcg cgtggttatc gttcgatgtg ccgcgtgtgg agcgggtgcc
      481 ggatgccgag cgagcgctca gtcatatggt cgggcgcttg atggatcagg aagacgggct
      541 caagaccctg ccggtctggg aacagtttct ctatgaagcc acgatgggca gtgatgcgaa
      601 cgatcgtgag caggccattg aatggtatcg cgaactcgcc gaagaatcgt ccgacccctc
      661 tgtcgatttg cacctggcga tcctggaggc cgagtcgggg taccttccgg ctgtgcagca
      721 gaaaatggct cgatgggttc ggcagggtga accgtacccg acctttgcca ggctcctgca
      781 ggccgggtac gtggatcctc gcgtgcctcc cgcctcgggc ttcgagctgc aggcttatct
      841 ggcggagcag tccgtgtcgg gctggttcta cagccgcctg gccactcgaa ttgccgaacg
      901 agcaggagac cggcccttgc tggtgacgat tgagacctcg ttgcagcagc gggtggaagc
      961 attgttgtgg cgctcccggg catttgccct gcttgaattg acgctcatga tcgtggggct
     1021 gttggtgctg gtgctgtggg tccggcgggg gcaaggcacg gcgatgtttc gcgtggggtc
     1081 cgcggagctt ccgcccctct gggccggtcg attgggcgcg ggggtgttac tacgcggggg
     1141 ggcggtgggc gccctgttga ccgtggcgtt tctctatgtc gccggcgatt atccatcgtt
     1201 gcgggtcgtc gcggtgccgt tgtcgaatct gcccctgctg gccttggcct actaccatct
     1261 gcttcggcct cagcggcaga cgttctggcg aggcttcggg ctacgcatcg aaccgcgaca
     1321 cttgggacaa ttggggctgg cggtgttggc ggtggtagcg gctggcctcg tgggcgaatg
     1381 ggtgctgggc aggattgcgg aaccgttgaa tttgatcagt cactggaccg agtggttcga
     1441 tgcggatctc gtgtggggat cgtcaccgac cctcatggtc agcctgatgg aatacgtgtt
     1501 ctttgcgccc gtctttgaag aactggcctt ccgcggcttg ttgttcggtg tattccgtcg
     1561 ccggtttcag tggggggtgg ccgccatgct gagcgcggcg ttgtttgcgc tcgcccatgg
     1621 gtatgggttg atcggattcc tcagcgtctt ctggagcggg gtgatttggg cctgggccta
     1681 tgaacggacc ggcagtctct ggccggggat gatcgggcac gccatcaaca atctgctggt
     1741 ttgtctgagt gtgatggcct tgttacgggc ctgagcaccg gtggtcttaa gcctcacagc
     1801 ctgatcgggg gccgtctgtc cgcttcgatc gactggagtg cgtcatagaa ttccgggtgc
     1861 cggagcgtat aacccacgtg tgtcaggatc ttgtgattcc ggatgcgttt gcctgattcg
     1921 ctgcgatccg cctgtcgagc actcaccact ccccacctgc cggagacttc cgcgcaaatn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gggccgcctt cccggtcata
     2101 cgcagaggtg ctgcccagca ccaccagcct tccgggaggg cgacaagagt gtcgggcaaa
     2161 tgcctggacc tgttcgagcg gtgtggcggg aaatgtccag atcaggtctg cgtcgggagg
     2221 aagtccggac caggtgctcg gctccgccag gtcgaatcga atgcgtcttg acggctcgac
     2281 atcactcaaa tggcgatcgg ggtgacggct gctggccagg atgggcaagg attgttgcac
     2341 ggccaacttg taaatccagc ggccggtata cccggatccc agaatgacga gagagcgcgg
     2401 cgtggtcatc ggcgcatcat acctgaacgg ctgatcggtt tgctgcggtc gactccggct
     2461 tattctggtt ctggtctccg gcgagcgacg catgctaaga tcctgcccct atgggaccct
     2521 tcagcgccag gcaggaggag acgagggaat cgagcaggca atcggccgat gacccgtcgc
     2581 atcggcgaca acgtctcatg gttggggcgg cgggcctgct ggcgtgtctc tttttcattc
     2641 tggatctcca gttgccgctc ggagtggcga acgcggtgct gtacagcgcc gtcgtctttc
     2701 tgtccgcggc cggccagtac cgatggctgc cgatcggcac agccatcacc tgctcgctgt
     2761 tgaccatcat ggccgctccg ttcagccccc gtgtgccggg ccttccggca tggtttgagt
     2821 ggggcaatca cctgttcagt ctgttcggca tctggactcc ggtcatgttt atctatcagc
     2881 gacggcgcac ggagttattg ctgaaggaag tcaacgagcg gcttgagcaa ggcgtggagg
     2941 cgcgcacggc ggacctggcc gcgagccgtc tggccttgga gcgaagcgaa gaacagctgc
     3001 atacgttgac cggtcggatt ctgacggcgc aggaagagga gcgccggcgg atcgcgcagg
     3061 atttacatga tgacgtcaat caacggctcg ccttgctgat gttggatttg caggcagtcg
     3121 accggcagat cggatccgct ccggcagaag ctcaggatgg tatccgcaac gctctgaagg
     3181 ggctggagga gttgtcggac gatgtgcggt ttatggcgta tcgattccat ccctccatcc
     3241 tggacgatct ggggttgaaa gcggccttgc agcggttgct ggatgacttt tcgagccgca
     3301 caggggtgaa ggcgctgttt gtgcaccaac cgctcgatca ccaactcgac aaaaccacat
     3361 cgaccgccgt gtaccgggtg atccaggaaa gcctgtcgaa tatcgcgcga catgccaagg
     3421 ccacccgggt ggaggtggag gtgacggtcg aagacgaggg gttggtcgtg gtggtgcgtg
     3481 acgacggcag agggttcgat cagcgggcga tcgatcgggc ggaaggcggc ttggggttgt
     3541 tgaacatgcg agaacgtttg ctggcggtgc agggatcctg cgaggtggaa tctaatccgg
     3601 gacggggaac gacggtgtcg atgtatgtgc cgttggtgcg ggtggcgaca tgaccctgcc
     3661 gcgcgtgctg ctggcggatg atcacaccct ggtgttggaa gggtttcggc ggctgctgga
     3721 tgaccagtgt gaactggtcg ggactgtcgg ggacgggcgt gccctactgg aggctattcc
     3781 ggagttgaag ccggatatcg tgattctcga catctcgatg ccggtgctga acgggatcga
     3841 tgcggcccgt gtcctgaagg tcaagtttcc ccaggtcaaa gtggtcttta tcacgatgca
     3901 tgccgatccg gcctatgtgc gggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnngaagat gtcacgtcca cgtgtcctca tcggcgattc ctcgcgggct atgctggcct
     4081 ttctggagat tctgttggaa cctcaattcg aggtcgtgtc ttccgaaact gaagcggaag
     4141 ccattgtatt gactgcaaaa aaaatcgcac cggagttggt cattctggat ttctccctct
     4201 cgtttctcga agggcttggg gctgcccggc agctgtatga gacaatgccg aacagcaagg
     4261 tcgtgttttt ctcttcgcat gaagaccctg tctacgtcac ggccgcgttc gaggcaggcg
     4321 ccaggggata tctcgtcaag catctcacgg tcgatctcgg gcattatctc ggccgggtgc
     4381 tgcaaggtga acgggtctgc tgtccggatg gtcttcacac gcgggtggct cgcacgaaag
     4441 acacctgacc gcttgctgtc cggccatgcg atggattcgc gcaaaaggag accgacgatg
     4501 gttcgagtcg taccgatgtg tgagttgtgc cgccgggttc gtgatgaggg ggcctcgacc
     4561 tgggggacca acagctgggt cgattttccc agttacctgg cgcgacatgt cgtgccgccc
     4621 tctcaggtgc ggttttcaag ggactactgc agtgagtgcc ggctctccta cgatattttg
     4681 aagacctacg gggggtaatg ggggcgaaag tcatcgctcg aaacattccc tccgaagcgt
     4741 ctagtgagca ttatcggtcg cgtcgtatcc caccagtcct gatcgtcccg gtttctctgt
     4801 tctgaacatc cccattgtcg ggatctccgc tgcctcgcct tccatttcct taatccgcat
     4861 ccgtggcggc ctgcgcgatg tgttgtcgtt tcctgttgtg ctacgatgat tctctgatgt
     4921 ctgccctgcc gtttcatccc atcgttgccg agtggttcac gacccgattc gcctctgcga
     4981 cgaatgtcca gatgcaggcc tggcctgcaa ttcaatccgg tcgtgacgtg ctgatctcag
     5041 cgccgacggg atcggggaag accctcgcgg cctttctctc ctgcatcgat actctctttc
     5101 agcaggcggt acgctgtgag ctgcgggatg agacgcaggt gctctatgtc tcgccgctga
     5161 aagcgctgag caacgatgtg cagaaaaatc tgcaacagcc gctcagcgag atcgggcagg
     5221 ccgcgttggc ttccggcatg ttgctaccgg aactgcgggt ggtcgtgcgt accggcgaca
     5281 cgcccatgac cgagcggcaa cagatgcttc ggcgtccgcc gcatattctg atcacgactc
     5341 ccgaatccct cttcatcctg ctgacggcgg aaaaaagccg ggcgatgttg aagaccgtgc
     5401 gcacggtcat cgtggatgaa attcatgccg tggcgcccaa taaacgaggg gcgcatctgg
     5461 ccctgtcgct ggagcgggtg gcggcgctca ccggcgtcga ggttcaacgc atcggcctgt
     5521 cggcgacgca gcggcccatc gaaaccattg cgaagttttt ggtgggaaat agaaacccgt
     5581 caatcgtcaa tggtcacacg tcatgcgaag ccgtcagtcg taagccatta gccatcagct
     5641 ctgcgccgtg cacgatcatc gacgtgggcc atcgccgcga tatggatctg gccgttgaag
     5701 tgccgaagga tgaactcggg gccgtcgcga cgaatgcgat ctggagcgat atctatgatc
     5761 gtgtcgccgc gctggtcgag gcgcatcgct ccaccctggt ctttgtgaat acgcggcggc
     5821 tggctgagcg ggtctcgcat tatttggaag agcggttgcg gcatcttggg gacgaggtgg
     5881 tggcggcgca tcacggcagt ctgtcgcgaa cgatccgcct gtcggccgaa gatcgattga
     5941 aaacgggcgc ggtgcgtgtg gtggtggcca cggcatcgtt ggaactcggc atcgatgtcg
     6001 gaaccgtcga tctcgtgtgc cagatcgggt ccccccgctc gatcgccacc ggactccagc
     6061 ggatcggtag ggcgggccat tggattcatg ccattcccaa gggccgtctc tttgccacca
     6121 cgcgtgacga attgatcgaa tgtgccgcct tgatccgggc gatcagggcc ggagtgctgg
     6181 accgcatcga ggtgccgcct gcgccgctgg atgtgttggc gcagcagatc gtggccgccg
     6241 cggcgacaca gtcgtgggat gaggcggagc tctacgatct gtgccggcgc gccatgcctt
     6301 atcgcgatct ggatcggtcg accttcgatg ctgttgtgac gatgcttgcc gatggttacg
     6361 tgaccagtcg aggccggggg cgggcctacc tccatcacga tcgcatcaat caccacattc
     6421 ggggccgtcg cggcgctcgc ctggcggcca tcacctcagg cggtgcgatc cccgacacgg
     6481 ccaactacgc cgtgattgcg gagccggacg gcacggtggt ggggtccgtc gacgaggatt
     6541 tcgccgtcga gagtctggcc ggagacatca tcctgctggg taacacgtcc tggcggatca
     6601 aaggcgtgga gaccggcaag atgcgggtcg aggatgcgca gggcgctccg ccgacgattc
     6661 cgttctggcg cggggaggcg ccggcgcgta cggcggaact gtcggcggaa gtggcgcgtc
     6721 tcaaggccga catcgatcat cgactgacta ccgaccaggc gctctccatg gcttctgcac
     6781 cgcccgtgca gtggctcagg caggaatgtg gtctcgacca gcggggggcg caacaggcgg
     6841 tcgaatatat cctggctggc aaggcggtgc tggggacggt cccgacgcag cagaccatcg
     6901 tggcggagcg gtttttcgat gaaagcggcg ggatgcagct ggtcatccat gcgccgttcg
     6961 gcgggcggat caatcgggcc tggggattgg ccttgcggaa gcggttttgt gtgaccttcg
     7021 actttgagtt gcaggccgcc gccacggatg aaggcattgt cctgtcgttg ggggagaagc
     7081 acagctttcc gctcgagacg gtgtttgcgt tcctgaacgt caacacgttg cgggaggtgc
     7141 tcacccaggc ggtgctgcaa gcgccgatgt tcatgacacg gtggcggtgg aacgcttcgc
     7201 gggccctggc cttgctccgg tttgtcggag ggaagcgggt gcccccgcag attcagcgaa
     7261 tgcgcgccga agatctcctt gcggcggtgt ttcccgatgc gattgcctgt caggataatt
     7321 tccagggtgc gcgaacgatc cggcagattc cggaccatcc gttggcgcag gagacgatcc
     7381 gcgattgtct gacggaagcg atggatctcg acggcttgat cgccgtgctg gagaagatcg
     7441 agcgcggcgc gatcgcctgt ctggcggtcg acaccccgat gccctcggcc ttttgccacg
     7501 aaattttgaa cgccaatcct tatgccttcc tcgatgatgc gccgttggag gaacgccggg
     7561 cccgtgcggt ggatatgcgg cgcagtttgc cgcctgaact ggcgggtggc atgggcgcgc
     7621 tggatcaatc ggccatcgat caggtcagtg aggagtcctg gccggtggtg cgggacgcgg
     7681 aggagtttca cgatgcgctg ttgtcgctgg gctgggtgcc ctgtgagcga atgcctggct
     7741 gggacttgct ggtgccgaag ctggcggcgg ccggtcgtgt cgctactctc tggcagggag
     7801 agacgaagct ggggtggctg gcaggggagt atcgccatta cgccgggttg ctgtttcccg
     7861 atgcgcggat cgatccggcg accggcccga ttgatccgac ggaacaggtt gagcaggaag
     7921 aggtcttgaa ccgggtggtg ctgggatgga tggagagtat cgggccgacg acggccgggg
     7981 aactgtcgca gacgctgcat ctgtccgagt cggacgttca gtccacgttt ctgcgactcg
     8041 agtcccaggg ccatctgctc cgtggccaat tccgtcagac actgagtgcg agatctgaaa
     8101 cgaaagatcc atcgtctcac ctttcacctt tcacttctca cgagttttgt catcgccgtc
     8161 tgctcgcccg gattcatcgc ttgacgatcg ggcggttgcg gagagaaatc gagcctgtca
     8221 cggccgccga gttcatgcgt ttcctgtttc agtggcaaca nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn cgtcgcgtga ttccgacccg ggtcgcaccc gtcggcatct
     8401 ttgcgcgcga agatgggccg gtcttgctgg cggccgccgg tgaggagttg gcgcgtctgg
     8461 atctttccgc ccgtttgagt ggctcggcac aggccattcg aggttgcctg caggcgcggg
     8521 gcgcgagttt tttcagcgag ctgttgcatg ggacgcggtt gctggcctcc gaagtggaaa
     8581 acgggttgtg ggaactggtc gcggccggcc tggtgaccgc cgacgggttc gacaatctgc
     8641 gttcgctgat cgatccgaag cggcggcgcg ccgaggcgtc ggatcgaagt cgccggccgc
     8701 gccatgtggg tggacgctgg tcgttgctgc gaccgatgga aaaccatcag ccatcatccg
     8761 gcagctctca gcagtcgaac tccgcaccgg ctacggagca cctggcgcgg caattgttac
     8821 agcggtatgg cgtggtgttt cgggatctgt tggggcggga gtcgatggtt tcatcctggc
     8881 gcgacctgtt ggtttgttac cggcggctcg aattgatggg ggaaattcgc ggggggcgat
     8941 tcgtgagtgg ctttacaggc gagcagtttg cgttgcccgg cgcattggaa gcgctgcggg
     9001 cgctcaagaa gcggcctggt gctgcgaccc agcaggacat caaaatttcc gccgccgatc
     9061 cgttgaacct cgcggggctc attctgccgg ggccccgtat tgccgcggtg ccgtcgaact
     9121 tcgtggtttt tcgagacggg atggtcgttc gtacggtgac gggccgcgag acgaacgacc
     9181 ggcaggtgtc gccggtcgtc gaggtcggtc gagtcggtgg ataggacgca gaccctgttt
     9241 cgtgcggtcc ggatcagatg agcttggccg gaatcgccag gagcgggcag cgcgcgccgc
     9301 ggagcacgcg ttccgtggtg ctgccgcgca gcatgtccag gaggctgtcc tgtgcctttg
     9361 tcgccatgac aatcaagtcc acgtcgaatt ctgccccggc cgccagaatc cattccaccg
     9421 gatcgccttt gccgaataat tgattccagg accagcctgc tttttgaggc agttccagcg
     9481 cgttgacgtc cggttctttc cccgcatgaa ccagggtcag cgtgacgggt ggggcgctca
     9541 gttccgagat cagcgcgctt gcggcatcga tggcgggctg ggaaggcggc tggatggaaa
     9601 tgggcaggag caggcgctgg agcgaggtct ggcctgtctc cctggaaaca aatccttcca
     9661 cgtgtgacgg gacaaacagc gtcttcacct gcatttcacg ggagaccggt tccgcgagcg
     9721 cgtgatgcac ccaccgggcg aatccctcgt gctgatgcgt ggccagcacc atcagatccg
     9781 tcggcgatgc cgtcagatga tgaatgatag cctgtttggc atcggaggcc aaggcgcgca
     9841 ctttcctgat ccggatgccc agtttggtca catcgccctt ggagctggat tccggcagca
     9901 gtccccactg agccagggtc gggcgaatac gggggaagtc ttcgaatccc tccggagaga
     9961 cggccgggtc cacatgcatg atcgtcagct cggcacgaaa caccagcgcc agcttgagtg
    10021 catgggcgaa ggccacctgg ctgtcctgcg aaaaatctgt ggggtggaag atccgatgaa
    10081 tcgtgaacgg ggggtgcaac gcattcatgt cgatgtcgaa ctccagtgag gtgctgtgat
    10141 cgtcactgct gccggctcgc cgaccggcgg gaacggctag cgcagcgcgg attgcagatc
    10201 gttcaccagg tcggtggtcg aggtgcttcc ggtccggctc cagcggctga atgagtcttg
    10261 cgcgaacggt tgaaataccg aggcgtcgga ggcgcccatg aggcggtcca gcgaggccag
    10321 gtactcgccc tgtccgcggg ccgcgtcgga ttgaagattg tccgcattgt aggcggtgaa
    10381 agccgcaacc ttgtgttcgg gtttcagcag gccgtcttcg ttccaccaga tgcgtccgga
    10441 tgtggtcccg gtaatgttcg aggtcgtgtc cgtcgtttcc ttgaaggtgg ctttgaagga
    10501 gcaggccgag agcagcaaga cgagagagag ccctccgaat gcggcgacga tcgggcgatg
    10561 attggcaatc atgcgtagac tcctttcggg aggcgaacag aatcgtgttg cgactatacc
    10621 atagtggaga ccaaattgag ccaggtgaga gatcgggtat gctcgtgcaa gtccggatcg
    10681 ttcgccggcc gtcaccgttg acaatagtgc ggagcatgag cacaatcgcc aaggccttga
    10741 tggggcagaa cttcgggcga ggcggtctgg ggggaacagc ggacccaggg gccggcgcac
    10801 cccgggaaga tgaggaggct tgaagatgaa gacacgcgtg ttgctgtatg ggcttgtcgg
    10861 cgtcaccctc ggtgttgcgc cggtgcgggc ggagtcggtt ccgatgaagc caggcgaata
    10921 tcaagtgacg gcggtgacgg atgccagcaa cggcgaggca gggaaaccgg acacccgcac
    10981 tcgctgtgtg agggaggagc atctcgccaa tccggatgcc gtctttaatt attatgcctt
    11041 gaacgggttt cagcccaacc cgtccagcaa ggtggtgaat gtgtcgatgc acggcgggaa
    11101 ggtcagttac gatatcgaag gcgtctacgc ggtcactcgc gtggagggga cggtgtctcc
    11161 caccgggttt tccgtggtcc gcaaagcgac gcccaagggc gatcatgcgc tgtcggtgac
    11221 catgaaagtc gacgcgaaac gaaccgggga ttgtcccggc aagtgatgcc gttcaggttg
    11281 cgcgttgaag ctacgatatg cctgtcgatg cggaggccgg gttgtcccgg cctcctgcgt
    11341 ttcgcctctt cccgctagtt cgtccacaag cgctgatacc atttcttctc ctcgccggca
    11401 ggcaccgagg cgatgttcat cgttttgcga atgtcaccga tgttccaacc cgtcagtgac
    11461 gccagcgttt gcgcctcccg ctcatatcgc tcccgcagtt gatgccggta cgccgtggcg
    11521 gcggcgcatt catcggttcc agtccaggga tggcgcagga tataacgcgc ctgaaaatta
    11581 ctgtgacccg aggtctcctg gaacatcagg tcttcaggga aatgcgcggc atcgtagcgc
    11641 acgtgcagcc gggtgaggaa cacgttctgg gccatcggta tgcccttgcc catccttccg
    11701 tgtccatcct gccagaagac gcccaaactt cggagttcat cggcagaaag cggattagcc
    11761 gcgcagggat cacaccagtt catgtcccag gcatattcca taaatacccc gcgctcgctc
    11821 tcccgtttca cctgctgcgt gaacaggtcg cgatagaagt cgccgaactt gtctttcacg
    11881 tagagcggaa tttcctgtgc ctcagggagt cgcacggttc ggtagttcgt cgtctctacc
    11941 cggccctgct tcgtcaggaa atagataaac agctcttgtg cgccatcggc gttcaccgtg
    12001 ccgagccgga tcggcaacat gaacttccgc gattcgaatg caatctgaag cggtcggagg
    12061 tgcgtcaagc cgagcttcgt ctgctcgccc agattcacct tggcgacgaa aaatttcatg
    12121 ccctgtttga gatagctatg gaggacggcc gacgctccgt tcgggatgcg gtagccgttc
    12181 tcgaccagcc aggtttcgag tccgttactt tctttggccg agaggatcag gatgtcgtat
    12241 tcgccgaccg tgtattgggc ttcgaccgtg acccctaagg ccttgtcacg ctcacgggcc
    12301 ggtgctgcag ccggggccat gctcttcatc gcatccatgc tcctacgctc catgagttca
    12361 taacgcaggc aggggttctc gtcgaagtat tcgaccagtc tcggcgccga gtagtcggcc
    12421 agatgtttca gtaccgccgg atcgccgaca tggatctgct ccttctccag gatggtcggc
    12481 accggaacca ccagcgcaaa ctccttcaca tctcctttga aatcgttcgc catggtgatc
    12541 acggttttgt tgtcgtggcg ggcgatggcc acttcagagg ccttgttgaa cagtctggtg
    12601 tcggccttgc cgacgtagaa accgcaaaag gccgaggcct cgccgctcca tgcgagcagg
    12661 ccgagggcga ataacaatga aggcacgatg gtacgtctca taagaacctc cttggttggt
    12721 gacacgagag aaacggagac agactgggga tgactaccgg ctgacggttg ggcattgatg
    12781 tgtaacgacg agtttggtga cgcgctacca gtcgatggcc tggtccaaac gtaacgagca
    12841 ccgggaaaca gatggtcgag tagtgggacg agtggtgtgc aggcgatcag cccccacagc
    12901 ggcccattcg ggcggaacaa tccgaactgg acgtaaagcg cggcgagggc aacgcagagg
    12961 gcatagacga ttcgcccggc gcgggagtcg ggtgtggttt tgggatcgga gatcatgaag
    13021 aaggcaaaga tcagcagcgc gccgctttcg atttgatgaa acgggattgt caacggatcg
    13081 ccgagccaca gggcccgtgc aaagaggaga ccgacgtaga cggcgagaaa ggcgaaggtc
    13141 acatccgccc gcgccgcccg ggtcacgacg agactgccga ggcaggcgat gagaaaaccg
    13201 aaccaggcga cctggcccca ttgtccaggt gagatccagc ccatcccgct cgcgagcgcg
    13261 atggccaacg ccaggttggt cggattgaac agatgtt
//
LOCUS       DAOQRM010000064        13149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3798_length_13149_cov_15.131129, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000064 DAOQRM010000000
VERSION     DAOQRM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3798_length_13149_cov_15.131129"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="PKK81_06880"
     CDS             complement(<1..1583)
                     /locus_tag="PKK81_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HNV32634.1"
                     /translation="MSSSQAQVALVNMPFSFSKYPSIQLGTLSALLKAKGIGVECHHL
                     NVRFAHKIGIPLYESICEKRALFGEWLFSYLLFRDNPKRAEYPRVFKPVFEQIARESG
                     QPISFFEEMATRTAPQFLTGAMTGIDWGQYKIVGFTSTFDQNVASLTMAKLIKDLYPD
                     VKIVFGGANYDGEMGLEYFRAFPFIDHVVVGEGEVTFPALVTHILSGSAGPCPKGVTY
                     REQGDIRCEPNTALFTEFAQTGPPDYDDYYHLLAELGTEASRGLDRILLYEGSRGCWW
                     GEKHHCTFCGLNAQSMKFRAKSSEQVAREMAYLSSRYDTTRFRLVDNIIDMKYVESLF
                     GRFAAEHCDLDVFIETKSNLQKSQIRTLAVGGVKCMQPGLESLSLSQLKAMDKGVTPM
                     QNIVCLKWSCYYRVSVSWNILLGFPGETNEDYRRQIDLLPSLFHLQAPEATGKFWLQR
                     FSPYFTRPHEYGVRITGPGTAYEYVYDAARVDLRKIAYDFEYELDDWPVDPHVYQELI
                     GLVEEWQRLARSSDRPFLY"
     gene            complement(1798..2145)
                     /locus_tag="PKK81_06885"
     CDS             complement(1798..2145)
                     /locus_tag="PKK81_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNV32635.1"
                     /translation="MSLADNKCIPCRGGVPPLPADRTQTLLQEVGRGWSLNAQGHLER
                     LYTFKDFAQSLAFANKVGAVAEAEGHHPDLYVAWGKCKVEIWTHKINGLTESDFYLAA
                     KADREFEPFRAGA"
     gene            complement(2232..2720)
                     /locus_tag="PKK81_06890"
     CDS             complement(2232..2720)
                     /locus_tag="PKK81_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV32636.1"
                     /translation="MLVQDVMSTGVVTARRNESVRSVVTKMLSRHCGAIPVVEDGELL
                     IGMVTLRDVLIPLYPNYGEYIHDNVHSRDFVEMEEGYADVLTQRVEEVMSLNPLTVTP
                     RTPVLEAASYMGLKNFRRIPVVEKGTLVGMVSVGDINRGLFFERGHAVMDEHRVAQPS
                     GR"
     gene            3041..3697
                     /locus_tag="PKK81_06895"
     CDS             3041..3697
                     /locus_tag="PKK81_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNV32637.1"
                     /translation="MGHRGAAGHAPENTLAAIWKARSFHADLIEIDLRATSDGHLVLL
                     HDDTIDRTTNKSGHVSEMSLEQIQRLDAGNWQRVPTLEEALDVAGRAIGMILELKVEG
                     IGNEACAIVKRTGFPGPLIYASFLMPELQRVRQTDPDAKIMVLQHRRLPPDPVADVVA
                     LDASHVGLHFSTVTPMLLQTYHNLGRQVFVYTVNESQDIQRMRNLGVDGIISDFPDRI
                     "
     gene            complement(3782..4318)
                     /locus_tag="PKK81_06900"
     CDS             complement(3782..4318)
                     /locus_tag="PKK81_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNV32638.1"
                     /translation="MSFLDVFVRGDFIDPATLVGAAFYAFLFILIAWFLARMIRLGVR
                     RVAYLLSDQAASTLLIRLGQVVVYTLAVILYFNAIPQLHSVGTALLASAGVASILFGL
                     AAQTTLSNLVSGFALLFYQPFEVGDRVQLSAPTGLETGVIDEMTMGYILVKTVDDRHI
                     VVPNSVAAQQIVVKLRAS"
     gene            complement(5462..6175)
                     /locus_tag="PKK81_06905"
     CDS             complement(5462..6175)
                     /locus_tag="PKK81_06905"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNV32639.1"
                     /translation="MRTVMLGLAGGVLLTAVAVVVSASGFEGLMLLNETSDGTTMQQQ
                     WFLKGDKLRFEETGPDAEKGAMIFDAKKKVMYSLQHGEKIYMEIPTGETSKAAPDRSE
                     DVVVIKTGKRDQAAGYPCEIYHTKDKSDGSTGELCIARGIGNAAMFGMMSAQAGGASL
                     LPGWMRELFKDGGFPIKGVDRDAKGNEEARWEVVNIEKKSLDDALFLPPADYKKQDMA
                     VIRQQSGDAMKEGRSPADR"
     gene            complement(6207..7088)
                     /locus_tag="PKK81_06910"
     CDS             complement(6207..7088)
                     /locus_tag="PKK81_06910"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNV32640.1"
                     /translation="MQRRWPIVVATVALSLAPLSARAGEFEGVLHMTTSHADTDMKSA
                     MDWYLKGDKGRMEMSRGEGRTSVMIFDATTGTMQMAMPGQKSYMEFSLAGERGEHLAQ
                     AFENQVVERTGKTETIAGYSCEIWRITDKEQHRLKSHVCVAKGFGKAATFWADPKDAR
                     RSSQPGWMKQLAEEGGFAIRSIQYDEAGKESMRMEVTSIDKKSLDAGLFAFPADWAKQ
                     DMSAMQERMKAMREQKGQGKADLSKMLEDMKKRKAARRGAGEPAGEAGPQPDMQEIMR
                     QLSEAMKKQQVQPQGGQ"
     gene            complement(7201..>7545)
                     /locus_tag="PKK81_06915"
     CDS             complement(7201..>7545)
                     /locus_tag="PKK81_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNV32641.1"
                     /translation="LAENGTAILKFFLHISKDEQKARLEARIHDPEKRWKWNSGDLEE
                     RKLWGEYMTAFEEVIAATSTEQAPWYIVPANRKWYRNLVVAELVVQALTAMKLSTPPA
                     PKGVNFKTLKIV"
     assembly_gap    7546..7645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7646..8189)
                     /locus_tag="PKK81_06920"
     CDS             complement(<7646..8189)
                     /locus_tag="PKK81_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNV32642.1"
                     /translation="MEKGDMKAYRIEPGCRLALKRFDPDDTGAYEKNEDGKAKAKAAA
                     DELIATLDQLQERLYANGSRALLIVLQGMDTSGKDGTIKHVMSGVNPQGCKVVTFKTP
                     SAEELSHDFLWRVHQKAPAKGQIGIFNRSHYEDVLITRVHGWVSDKVVTRRFNQIKEF
                     EELLAENGTAILKFFLHISKD"
     gene            8409..8894
                     /locus_tag="PKK81_06925"
     CDS             8409..8894
                     /locus_tag="PKK81_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNV32643.1"
                     /translation="MLKEVTGDILLSTAGAIAHGIAPHDNFKQGLALSLREQWPGMYK
                     DFRHYCQTYNPKPGGAWSWKGPNSPVIINLFTQEPPASDQDHPGKATLPNVNHALQAL
                     KKELQEHQVKSVALPRLATGVGGLEWEKVYPLLQQALKDLNIPVYVYGQYKKGVTAQE
                     A"
     gene            complement(8938..9702)
                     /gene="otsB"
                     /locus_tag="PKK81_06930"
     CDS             complement(8938..9702)
                     /gene="otsB"
                     /locus_tag="PKK81_06930"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNV32644.1"
                     /translation="MLSTPGKRVLDRLAGQAEALFAFDFDGTLARIVQDRHAAFLTHP
                     IREALHALAVTAPTAVISGRSLADLSPRVDGIPAHLIGNHGLEGLHTSERVMHQAQDC
                     CRAWLKTVSKDERNLTRAGVVVEDKTYSVTFHYRQACSPQLAREAIFHTVSMLAPAPR
                     LVLGKAVVNAIPSGNLHKGSAMLELMHQLKSSGALYVGDDDTDEDVFSLPDERIVTVR
                     IGKKAASAAQWYLARQSQIGLLLQYLTRIRGRLASA"
     gene            complement(9720..11951)
                     /locus_tag="PKK81_06935"
     CDS             complement(9720..11951)
                     /locus_tag="PKK81_06935"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNV32645.1"
                     /translation="MRLSLRFILPLLLVLGVVAYSVVPLVDSLTLKWFTRDLESRSKL
                     LASTMEVPLADLVVSRSRTKIFAYFHKVIQDERLYALGFCDTQQRLLYQTLTYPDQLS
                     CESLAHLAPGSSEVVEFAQGPLHVVVATIQSGGKAQGRLMLVHDMSFVHQRSTDTKWY
                     IFYLFVGLAGLISAVTVLVAQLSWRGWVAGVRAMLTSKGLPPESAGAHSSELHPVAQD
                     LHALVRELEADRRMRDESQITWSPASLKTILHEHLSGDEILIVSNRQPYIHNRRGQKI
                     EVQVPASGLVSALEPVMRACSGVWIAHGNGSADREVVDSRDHVRVPPDHPSYDIRRIW
                     LSPDEESGFYYGFSNEGLWPLCHNAHVRPTFRTSDWEQYVAVNERFAKAVVEEAKTDD
                     PVILVQDYHFALLPKMVREKLPNATIIMFWHIPWPNSESYGICPWRQEILEGLLGSSI
                     VGFHTREHGNHFLSCIDRILEARIDRDSSTVSYGGRLTAVNPYPISIEWPSRWLENQR
                     PVPDCRVHIRERHAMGPDRLVGIGVDRLDYTKGIIERFLAVERLFELQPEWVGKFSFI
                     QIAAPSRTIIDQYQHFHDQVYALAERINKRFGREGYEPIVLKVKHHEPPDVYEYYRAS
                     ELCFVTSLHDGMNLVAKEFIAARDDEQGVLILSQFTGAAQELPEALVVNPYDIDQCAA
                     ALHMALSMPPKEQRARMRSMRGLIQEFNVYRWAGRMLMDAARMRRRIRVMKQVGQASG
                     RGR"
     gene            12002..>13149
                     /locus_tag="PKK81_06940"
     CDS             12002..>13149
                     /locus_tag="PKK81_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNV32646.1"
                     /translation="MYPYGLIGNCHVSAHIHESGSVDWLCLPRPDSDPVFGRILDPEG
                     GHFSISSPTSSAQVKTTQRYLPNTNILVTEVSTSKGDTFRITDFCPRFEQYGRVYRPA
                     ALFRIVEPLAGTPSIRVSCRPVTGWGKEYARGMRGNSHVRYDIRGEYLRLLTNMPLTY
                     LYEERPFALTEKTYFALTWGLGIEDDLAKVSHEFLDQTTRYWRMWVKHCSIPVLHQEE
                     VIRSALALKLHCYEDTGAILAALTTSLPEEPGGPRNWDYRYCWLRDAYFSLTAFHNLG
                     HFEEMEGFLKFLLNIAYTHEHSRERLAPVYTLSQDLPLPETEHRNWAGFCGSAPVRNH
                     NQAAEHIQNDVYGELVLALTPIFSDNRFYDLRTKDQEQLVANLARLCDR"
BASE COUNT         2737 a         4156 c         3554 g         2602 t
ORIGIN      
        1 taatacaaaa acggccggtc gctgctgcgc gccagccgct gccattcctc caccaaaccg
       61 atcaattcct gatagacatg gggatccacc ggccagtcgt cgagttcata ctcgaaatcg
      121 taggcgatct tgcgcagatc gacgcgcgcc gcatcataca catattcata ggctgtcccc
      181 ggacccgtga tgcgcacgcc gtactcatgc ggtctcgtaa agtagggact gaaccgttgc
      241 aaccagaatt tcccggtcgc ctccggcgct tgcaaatgga acagggacgg caggagatcg
      301 atctgccgcc gatagtcctc attcgtctcg ccgggaaagc cgagcagaat attccaggat
      361 accgacactc ggtagtaaca actccacttg aggcacacga tgttctgcat gggcgtcacg
      421 cccttgtcca tggccttcag ctgtgacagg ctgaggcttt ccaaaccggg ctgcatgcac
      481 ttcacgcccc ccacagccag cgtccggatc tggctcttct gcagattgct cttggtttcg
      541 ataaacacat ccagatcgca gtgctcggcg gcaaaccggc caaacaggct ctcgacatat
      601 ttcatgtcga tgatgttgtc caccagccgg aaccgtgtcg tatcgtagcg actcgacaga
      661 tacgccattt cgcgagcgac ctgttcagac gacttggcgc ggaatttcat actctgcgca
      721 ttcagcccgc agaaggtgca atggtgcttc tcaccccacc aacaaccgcg tgagccttcg
      781 tacaatagaa tacgatctag gccgcgcgac gcctcggtgc cgagttcggc cagcagatga
      841 taatagtcgt cgtagtccgg cgggccggtc tgtgcgaact ccgtgaagag cgcggtattc
      901 ggctcacatc gaatgtcgcc ctgctcccga tacgtcaccc ctttcgggca gggcccggcc
      961 gatccgctca agatatgggt caccaatgcg gggaacgtca cctcaccttc acccacgacc
     1021 acatggtcga tgaacggaaa ggcgcgaaag tattccaacc ccatctcgcc gtcgtagttg
     1081 gcgccaccga acacgatctt cacgtccgga tacaaatcct tgatgagctt cgccatggtc
     1141 agactggcca cgttttgatc gaaggtggac gtgaacccca cgatcttgta ctggccccaa
     1201 tcgatgccgg tcatggctcc ggtcaggaat tgcggcgcgg tccgcgtggc catctcctcg
     1261 aagaacgaaa ttggctggcc gctctcccgg gcaatctgct cgaacaccgg cttgaacacc
     1321 ctcggatact cggcccgttt ggggttgtcg cgaaacagca gataggagaa gagccactcg
     1381 ccgaacagcg cgcgtttctc gcaaatcgat tcgtagagag gaatcccgat cttgtgcgcg
     1441 aatcgcacat tgaggtgatg acactcgacc ccgatgccct tcgctttcaa cagcgccgac
     1501 aacgtgccca attgaatgga aggatacttc gaaaaactga agggcatgtt gacgagcgcc
     1561 acctgggcct gggagctact cataagatcc ccagtccagc cggcacaagg cgggggccaa
     1621 acccggagaa gtcatggaca tcgtcacgag acagtcgaga ccgaagctcg ccgcgggcgc
     1681 ccgcagagag gacgacgcag gcgtattcac cgcaatccgt cgaggaggcc gagcgagaac
     1741 gtccgcgccg agcgagagga tcggctgtcg cagtagatgc gccatgattt ctcctggtta
     1801 ggcccccgcc cggaacggtt caaactctcg atccgctttg gcggccagat aaaaatcgct
     1861 ctccgtgagg ccgttgatct tgtgtgtcca gatctcgact ttacattttc cccaggctac
     1921 atacagatcc gggtgatggc cctcggcttc agccactgcg ccgaccttat tcgcaaatgc
     1981 cagcgactgc gcaaaatcct tgaatgtata aagccgttcg agatggcctt gggcattcag
     2041 cgaccaccca cgccccacct cttgcaacaa ggtctgtgta cgatcggcgg ggaggggagg
     2101 gactccgcct cgacacggaa tacatttatt gtcggccaga ctcatgacat ctccttcgca
     2161 gacggtgaca gccgtcggtc gtttcaacaa ggggctattc tacgagttgc ccgttcggtc
     2221 ccgcaaccgc cctatcgccc tgacggttgt gcgaccctgt gttcatccat cacggcatgc
     2281 cctcgttcga aaaagagccc tcgattgatg tcgcccacgc tgaccatccc gaccaatgtc
     2341 cccttctcga ccaccggaat ccggcgaaaa tttttcaagc ccatatagga cgcggcttca
     2401 agcaccgggg tccgtggcgt cactgtcagc ggattcaggc tcatgacctc ctcaactcgt
     2461 tgagtgagga cgtccgcata gccctcctcc atctccacaa aatcccggct gtgcacattg
     2521 tcatgaatat attcgccgta attgggataa agaggaatca agacatcgcg cagcgtcacc
     2581 atgccgatca agagctcacc gtcctcgacg accggaatgg cgccgcaatg acgactcagc
     2641 atcttggtga ccacgctacg gaccgactca tttcgcctgg ccgtgaccac cccggtagac
     2701 atgacatcct ggacaagcat ggcagcctcc tttcacacag ggcgactcgc agaggagtca
     2761 ccggaagggg aatgtattct gcatcatcac tcgctctgat ccaagtcacc tccggccaca
     2821 tcatccgatc ctggcagagc cgggaccggg tccgccgctc accatcgtac caactcaccg
     2881 gagggtgggc ccctcggcgt tctttgcaga cttacgcgaa atatggtagg gaagtggggt
     2941 tcgaaaagaa agcccctccc tgcagccggt caacgcagga tcggggaggg ccctctccca
     3001 gggcatctca cgcgaggtcg ctatcgccac cgtccttcgc atcggtcatc ggggagccgc
     3061 cggccacgcc cccgagaaca ccctcgccgc tatttggaaa gcccgctcgt ttcacgcaga
     3121 tctcatcgaa atcgaccttc gcgcaaccag cgacggccat cttgtgttgc tgcacgacga
     3181 caccatcgac cgcaccacca acaagagcgg acacgtgtcc gagatgtcgc ttgagcaaat
     3241 ccaacggctc gatgcgggga actggcagcg tgtccccacg ctggaggaag ccttggacgt
     3301 tgcgggacgc gcaatcggta tgattctgga actcaaagtg gagggaatcg gcaacgaggc
     3361 ctgcgcgatc gtcaagcgca ccggcttccc cggaccgttg atctatgcgt cgtttctgat
     3421 gccggagctg cagcgcgtgc ggcaaaccga tcccgatgcg aagattatgg tcttgcagca
     3481 ccgccggctg ccgccggacc ctgtagccga tgtcgtcgcg ttggatgcct cccatgtcgg
     3541 actccacttt tcgacggtca cacccatgct gctgcagacg tatcacaacc tcggacgaca
     3601 ggtctttgtc tacaccgtga atgaatcgca agacatccaa cgcatgcgca acctgggcgt
     3661 ggacgggatc atctccgact tcccggaccg catctgagct cggcacagag cagatggcct
     3721 acggctcgga aagagaacgg tcccgtcact gtcttggcta taagccattt gctatttgct
     3781 cttaagacgc acgtagcttc accacgatct gctgggcagc gacgctattc ggcaccacga
     3841 tatgacggtc atcgaccgtc ttcaccaaaa tatagcccat ggtcatctca tcgatcacgc
     3901 cggtttcgag tccggtcggc gcagacagtt gcacacgatc gcccacctcg aacggctgat
     3961 agaacagcaa ggcgaagccg gacaccagat tgctgagcgt ggtctgcgcc gccaagccga
     4021 agaggatcga cgccacaccg gcactcgcca gcaacgccgt ccctacggag tgcagttgtg
     4081 ggatcgcgtt gaagtagaga atgaccgcca gggtatagac caccacttgc cccagcctga
     4141 tcagcaacgt cgaggccgcc tgatcgctca gaagatacgc cacccggcgc acgcccaacc
     4201 tgatcatacg agcgaggaac caggcaatca ggataaataa gaacgcgtaa aacgcagccc
     4261 cgaccaaggt ggcaggatcg atgaaatctc cgcgaacaaa cacgtcaaga aaagacatag
     4321 ggacctccca tggcacggca cgtgattcag aatcagcaac tcttgggact gtatgccatg
     4381 gaagaaggac ggaacaaggg cggaagaccg ggtcgcataa aatgccacgg gcgaccctac
     4441 actgcggtgg gaggtcgccc gtggatggcg cttgggacgc cgaaagtcga ttatattgtc
     4501 ggaaactcag gacccgttcg agaagacagg gtatcaggat gtccgtcggg ggataatcta
     4561 tcccaggaaa cttgtgtgcc tcgtgtttgg atcgaatcct tctctttttg ggtggctgtt
     4621 atccggttat cggatagtgc cgagaggcac aagccagcga aggatcgtca aacccgaccg
     4681 acgatcagcc caccctcaac tctgcgggcc gccctagtcc ggtggcgtaa gttcttccat
     4741 ctgtgaacct ccccggttga ggagtggcga ggtctcccga acaggcgcgc actgtccatc
     4801 gcctcgtcac ggttaacaca gcccttggaa tacacaatcc cgtcttacgc tccttagaaa
     4861 atcctgtcaa tagaaaaatc ggccctagtc atatttcctg atccgaatcc gataggccgg
     4921 ttcgagctct cgttacggca agggcccctg cttggacagg agcccttggg tggcgcttag
     4981 gacgccgagt gctgtccgtg atgtagcggc ccgtgaagac ccgccgagtc gaacaagagg
     5041 gaagaacatc tcgccggaga aaaacagcct gtcatcaaca ggcgacgtgt agagatcaaa
     5101 accagttctc cgcttagggg catgacgcga tgatggagat cggtgcgtct tcgatgccgc
     5161 gaggcatagc cgagagagaa ccggccgaca gaaccgacga gacgcttgcc ttcaatccgg
     5221 caggccgtac gggccaggtg gagtcgtatc gttcatcgtc caccctcctt tccgttgcgg
     5281 gactcagtgg gagatctaga cggcagccgc gcggtctgtc cggtctccca gctaccacga
     5341 tggagtcggg ccgcaaatcc tatctacgct cctcgaaaag ttctgtcaat acagggtgtc
     5401 gttggagtcc cgatggcggc ccgaccgacc gccatcatcc ggtggcaggc agcatgcagc
     5461 cttaccggtc cgccggtgac cgcccctctt tcatcgcatc gccggactgc tgcctgatca
     5521 cggccatgtc ctgtttcttg tagtcggcgg gagggaggaa caatgcatcg tcgaggcttt
     5581 tcttttcgat gttcacgact tcccagcgag cctcctcatt ccccttggcg tcacgatcca
     5641 cacccttgat cggaaacccg ccgtccttaa acagctcacg catccacccc ggcaacaacg
     5701 aagcaccgcc cgcttgcgcg ctcatcatcc cgaacatggc cgcattcccg attcccctcg
     5761 cgatacagag ctcgccggtg ctgccgtccg acttgtcttt cgtgtgatag atctcgcacg
     5821 gataccccgc ggcctggtcg cgttttccag tcttgatgac tacaacatcc tccgacctgt
     5881 ccggcgcagc cttggacgtc tcccccgtgg ggatctccat ataaatcttt tcgccgtgct
     5941 gaaggctgta catcaccttc ttctttgcat cgaagatcat ggcgcctttt tccgcatccg
     6001 gacccgtttc ctcaaaccgc agcttgtcgc ccttcagaaa ccactgctgc tgcatggtcg
     6061 tcccgtcgct cgtctcattc aggagcatga ggccctcaaa accactcgcc gacacgacta
     6121 ccgcaacagc cgtcagcaag acgcctcccg ccagacccag catgaccgta cgcatgtcgc
     6181 tctcctttca gtccggatgc gcggagctac tgcccgcctt gcggttgtac ctgctgtttt
     6241 ttcatcgcct cgctgagctg cctcatgatc tcttgcatat ccggttgcgg cccagcctca
     6301 ccggccggct cacccgcacc gcggcgcgcg gctttgcgct ttttcatgtc ctccagcatc
     6361 ttggagagat ccgccttacc ctgccccttt tgctcgcgca tcgccttcat ccgttcctgc
     6421 atcgccgaca tgtcctgctt cgcccagtca gccgggaagg caaagaggcc ggcatccaag
     6481 ctctttttgt cgatgctggt cacttccatg cgcatggatt ccttgcccgc ttcgtcatac
     6541 tgaatgctcc ggatggcgaa tcctccctcc tcggcgagtt gcttcatcca gccgggctgc
     6601 gaagaccgcc gagcatcctt cgggtcagcc cagaacgtcg cggcttttcc gaaccccttg
     6661 gccacacaga cgtggctctt cagtcgatgc tgttccttgt cggtgatgcg ccagatctcg
     6721 caggaatatc ccgcaatcgt ctcggtcttc cccgtccgct cgacgacctg attttcgaac
     6781 gcctgcgcga gatgctcccc acgctcgccc gccaggctga attccatata agacttctgc
     6841 ccgggcatcg ccatctgcat cgttccggtc gtggcgtcga agatcatgac gctcgtgcgc
     6901 ccttcgccgc gagacatttc catgcggccc ttgtcaccct tgagatacca gtccatcgcg
     6961 ctcttcatat cggtgtccgc gtgactggtc gtcatgtgga gcacgccttc aaactcaccg
     7021 gcgcgggcag agagtggagc gagactcaag gcaacggtcg cgacaacaat cggccatctt
     7081 ctctgcatga tccacactcc tggttagacg agcgcctgca agacaaactg cagtgtagcc
     7141 gtgtgtagtg gccggcacca gcctggaccg gccaggcttg cccctgcgag caaccacgcc
     7201 ctaaacaatc ttcagcgtct tgaaattcac tcccttcgga gccggagggg tcgagagctt
     7261 catcgccgtc aacgcctgaa caaccagttc cgccaccacg agattgcgat accactttcg
     7321 attggccggc acgatgtacc agggggcctg ttcggtgctg gtcgccgcga tcacctcttc
     7381 gaacgccgtc atatactcgc cccacagttt gcgctcttcc agatccccgg aattccattt
     7441 ccaccgtttt tcgggatcgt gaatgcgggc ttccaggcgg gccttctgtt cgtctttcga
     7501 aatatggagg aaaaatttca gaatggccgt cccgttctcg gcaagnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnncgtct ttcgaaatat ggaggaaaaa tttcagaatg
     7681 gccgtcccgt tctcggcaag cagttcctcg aattccttga tctgattaaa gcggcgggtc
     7741 acgactttgt ccgagaccca gccatgcacc cgcgtaatca acacgtcttc atagtgtgaa
     7801 cggttgaaga ttccgatctg gcccttggcg ggcgccttct gatgcacgcg ccagaggaag
     7861 tcgtgggaga gctcttctgc ggaaggcgtt ttaaaagtca ccaccttgca gccctgcgga
     7921 ttaaccccgg acatgacgtg cttgatcgtg ccgtccttgc cgctggtgtc catgccctgt
     7981 agcacgatca acagggcgcg cgagccgttg gcatagagac gctcctgcaa ttgatcgagt
     8041 gtcgcaatga gttcgtccgc tgccgccttg gccttcgcct ttccatcttc gttcttctcg
     8101 tacgccccgg tgtcatcggg atcgaaccgt tttaatgcca gtcggcagcc gggctcgatg
     8161 cgataggctt tcatgtcacc cttctccaca atagtgcctc cttccactga ccaagaccag
     8221 cgcttcctcg cacgatctct tcatgaaaca gaggcctgca cgccgacatg gcgagcgact
     8281 gcctcggcga cagcacgagc acactcccgg tcgcgaatta aatttcttcc cccgctccat
     8341 tgattgaacc ggacgcagcc ttatgtgata agaaatgacc ttatccccac atcaaggagg
     8401 cgtcagtcat gctcaaggaa gtgaccggag acattctctt atccaccgcc ggcgcgatcg
     8461 cacacggcat tgccccccac gacaatttca agcagggctt ggccttgtcg cttcgtgagc
     8521 agtggccggg catgtacaaa gattttcgcc actattgcca aacctacaat cccaaaccgg
     8581 gcggcgcctg gtcctggaaa ggcccgaatt ccccggtcat catcaatctg tttacgcaag
     8641 agccgccggc cagcgatcag gatcatcccg gaaaggccac cttgcccaac gtcaatcatg
     8701 cgctccaggc gttgaagaaa gaactgcagg agcatcaggt gaagagcgtg gcgctgcctc
     8761 gtctggcaac gggagtcggc ggactggaat gggagaaagt ctatcccttg ctccagcagg
     8821 ccttgaaaga cctgaatatt cctgtgtacg tctacggtca gtacaaaaaa ggagtgacgg
     8881 cccaggaagc ctagcaggcc ccaccggttc ctgcgccggg ccgatgagta tcaggagcta
     8941 ggccgacgcc agacggcctc ggatccgggt cagatattgc agcaacagtc cgatctgcga
     9001 ctgccgcgca agataccatt gtgcggcaga cgcagccttc tttccgatgc gcaccgtgac
     9061 gatgcgctca tccggcaacg aaaacacatc ctcatcggta tcgtcatcgc cgacatacag
     9121 agcaccggaa ctcttcagct ggtgcatcaa ttccaacatc gccgacccct tgtggagatt
     9181 gccggagggg atggcattca ccacggcttt gcccaacacg agcctgggcg caggcgccaa
     9241 catcgacacg gtatgaaaga tcgcctcacg cgcgagctgc ggtgaacaag cctgccgata
     9301 atgaaacgtg accgaatagg tcttgtcctc caccaccact cccgctcgcg tcagatttcg
     9361 ctcgtctttg gacaccgtct tcaaccaggc gcggcaacaa tcctgggcct ggtgcatgac
     9421 ccgctccgac gtatgcagcc cttcgagccc gtgattgccg atgagatgcg cggggatgcc
     9481 gtccacgcgc gggctgaggt cggcaagtga gcgtcccgaa atgaccgccg tcggcgccgt
     9541 cactgccagg gcgtgcaggg cctcgcgaat agggtgggtg aggaaagcgg catgccggtc
     9601 ttgaacgatt cgcgccagcg tgccgtcgaa atcgaacgca aacaacgcct cggcctggcc
     9661 ggccagccga tcgaggacac gcttgcctgg agtgctgagc acgtcctgca tcggtgcgat
     9721 taccgccccc gtcccgaagc ttgtcccacc tgcttcatca cgcggatacg acggcgcatc
     9781 cgggccgcgt ccatgagcat acgaccggcc caccggtaga cattgaactc ctgaatcagt
     9841 ccgcgcatgc tccgcatacg ggcgcgctgt tccttcgggg gcatagagag cgccatgtgc
     9901 aacgccgccg cgcactggtc gatatcgtag ggattcacca ccaacgcctc cggcagctcc
     9961 tgcgcggccc cggtgaactg gctgagaatc agcacgcctt gttcgtcgtc gcgcgccgcg
    10021 atgaattcct tggccaccag gttcatgccg tcatgaaggc tcgtgacgaa gcagagctcg
    10081 gaggcccggt agtactcata cacatccggc ggctcgtggt gtttcacctt caacacgatc
    10141 ggctcatacc cttcgcggcc gaatcgctta ttgatgcgct cagccagcgc gtagacttga
    10201 tcgtggaaat gctggtactg gtcgatgatc gtccggctcg gcgccgcgat ctgaatgaag
    10261 gaaaacttgc cgacccactc gggctgcagc tcgaacaatc gttcgacggc gagaaatcgc
    10321 tcgatgatgc ccttggtgta gtccaagcgg tcgacaccga tcccgaccag ccgatcgggt
    10381 cccatcgcat gacgctcgcg gatatgcacc cggcagtccg gcaccggccg ttgattctcc
    10441 aaccagcgcg acggccactc aatggagatc gggtagggat tgaccgccgt gaggcgcccg
    10501 ccgtaggaca ccgtggagct gtcgcggtcg atgcgcgctt caaggatgcg atcgatgcag
    10561 gacaggaaat ggtttccatg ctcacgggtg tggaacccga cgatgctgct gccgagcaag
    10621 ccttccagaa tctcctgccg ccacgggcag atgccgtagc tctcggagtt gggccaggga
    10681 atatgccaga acatgatgat ggtggcgttc ggcaacttct cacgcaccat cttcggaagc
    10741 agcgcaaagt ggtaatcctg tacgaggatc accggatcat ccgtcttggc ctcttccacc
    10801 actgccttcg cgaaccgctc gttgacggcc acatactgtt cccagtcgga agtcctgaac
    10861 gtcggacgga catgcgcatt gtggcataag ggccagaggc cctcgttgga aaacccataa
    10921 taaaacccgg attcttcgtc gggcgacaac cagatgcggc gaatgtcgta agacggatgg
    10981 tccggcggca cccgtacgtg gtcccggcta tccacgactt cccggtccgc tgaaccgttg
    11041 ccgtgagcga tccacacgcc cgaacaagcc cgcatcaccg gttcgagggc ggacaccagc
    11101 ccgctggcag gcacttgcac ctcgattttc tgcccgcgcc gattgtggat gtagggctgg
    11161 cgattggaga caattagaat ttcgtcgcca gacagatgct catgcagaat ggttttgagg
    11221 ctggcgggac tccaggtgat ctggctttca tcgcgcatcc ggcgatcggc ttccaattcc
    11281 cgcaccagcg catggagatc ctgcgccacc ggatgcagtt cggacgaatg ggctccggcg
    11341 gattcaggcg gcaacccttt gctggtcaac atggcccgca ccccggccac ccagcctcgc
    11401 cagctgagtt gcgcgacgag cacggtcacc gcggaaatca acccggcgag tccgacgaag
    11461 agataaaaga tgtaccactt cgtatcggtg ctgcgctggt gcacaaaact catgtcgtgg
    11521 accagcatca gccggccctg cgctttgcct ccggactgaa tggtcgcgac caccacgtgc
    11581 aacggtcctt gcgcaaactc caccacttcc gaagagcccg gcgccagatg cgccagactt
    11641 tcgcaggaca gctggtcggg ataggtcaac gtctggtaga gcagacgctg ctgggtatcg
    11701 cagaaaccca gcgcatagag ccgctcgtcc tgaatgactt tgtgaaagta ggcgaagatc
    11761 ttcgtgcgtg aacgcgacac gaccaggtcc gccaacggca cttccatcgt gctggccagt
    11821 agtttggatc ggctttcgag gtcgcgcgtg aaccacttca gcgtgagcga atcgacgagg
    11881 ggcacgacgc tatacgccac gactcccaaa accagcaaga ggggaaggat aaaccggagg
    11941 gacagccgca tttttgctcc tgcagtttac tttgagacga aaatctctta tggtcggggg
    12001 catgtacccc tacggactca tcggcaattg ccacgtctcc gcccatattc atgaaagcgg
    12061 gtctgtggac tggctctgtc tgccgagacc ggatagcgac ccggtcttcg gtcgaatcct
    12121 cgatccggag ggagggcact tttccatatc aagccctacc agctccgctc aagtcaagac
    12181 aacgcaacgc taccttccta atacaaatat cctggtgacc gaggtctcca cgtcaaaggg
    12241 cgacaccttc aggatcacgg acttctgccc gcgcttcgaa cagtacggcc gcgtgtaccg
    12301 tcccgccgcc ctctttcgca tcgtcgaacc gctcgccggc actccctcca tccgcgtgag
    12361 ttgccgcccg gtcacgggat ggggaaaaga atatgcccgc ggcatgcgcg gcaacagcca
    12421 cgtccggtac gacatccgcg gggagtacct ccggttgttg accaacatgc ccctgaccta
    12481 cttgtacgag gaacgcccct tcgccctcac cgagaaaacg tactttgcac tcacctgggg
    12541 cttgggcatc gaagacgatc tcgccaaggt cagtcacgag ttcctcgacc agacgacccg
    12601 ctactggcgc atgtgggtga aacattgctc gatcccggtg ctgcaccagg aagaagtcat
    12661 tcgttccgcg ctcgcactga aactccattg ttatgaagac acgggtgcca tcctggccgc
    12721 cttaacgacc agcctcccgg aagaaccggg cggcccacgg aattgggact atcggtactg
    12781 ctggctgcgc gacgcctatt tttctctgac cgccttccac aatctcgggc acttcgaaga
    12841 gatggaaggg tttctcaagt tcctgctcaa catcgcctac acgcatgaac attcgcggga
    12901 acgactcgcg cccgtgtata cgctcagcca ggatctcccg ctgccggaaa ccgaacaccg
    12961 gaactgggcc ggcttctgcg gaagcgcgcc ggtgcgcaat cacaatcagg ccgccgaaca
    13021 tatccaaaac gatgtctacg gcgaactggt cctcgcactg accccgatct tctccgacaa
    13081 tcgtttctac gatctgcgga ccaaagatca ggaacaactc gtcgccaacc tcgcccgact
    13141 ctgcgaccg
//
LOCUS       DAOQRM010000065        13133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3808_length_13133_cov_20.162028, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000065 DAOQRM010000000
VERSION     DAOQRM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13133
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3808_length_13133_cov_20.162028"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKK81_06945"
     CDS             complement(<1..377)
                     /locus_tag="PKK81_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32647.1"
                     /translation="MKDLQVTVVGLARSGVGAARLLNHLGARVTLADRKEAQELTAIL
                     SQVDRSSIAVKVGVQYESALEGADLVVISPGVPTQLDALNRVRARGVRVIGELELASR
                     FLTAPVVAVTGTNGKSTTVTLIG"
     gene            complement(394..1470)
                     /gene="mraY"
                     /locus_tag="PKK81_06950"
     CDS             complement(394..1470)
                     /gene="mraY"
                     /locus_tag="PKK81_06950"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNV32648.1"
                     /translation="MLYNWLYPLHTQFSFLNVFRYQSFRIIYAAVTAFLIAFVMAPWV
                     IRKLQEIKLGQQIRDDGPKRHLAKSGTPTMGGILIIFAVVLSTLLWADMTNRYVWLVV
                     VATVGFGAIGFADDYLKFIKRQSKGLSAAQKFTCQILVALTIGVFLYTLPSYTTKLSV
                     PFFKFFTPDLGWFYIVFVILVIVGSSNAVNLTDGLDGLAIGPVMIASLAYTIVAYVTG
                     NRVMAEYLLIPYIEGAGEIAIFTGAILGSSLGFLWFNTYPASVFMGDVGSLPLGAALG
                     TVAVISKHELLLLLVGGVFVIEALSVILQVGSYKLRGKRIFNMAPIHHHFEMKGWDEP
                     KVVVRLWIIAILLALLSLSTLKLR"
     gene            complement(1535..2998)
                     /gene="murF"
                     /locus_tag="PKK81_06955"
     CDS             complement(1535..2998)
                     /gene="murF"
                     /locus_tag="PKK81_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNV32649.1"
                     /translation="MREHGNRGVMALFTVEEICEVLSVRPPAGVTPQDLKQRIRRVVT
                     DSRLVRKGDLFIAFQGERFDAHTFVPKVLAQGAVCAIVQEDYRLPPAPKRTGVPMLLG
                     VRDTLEAYQRLATHYRNRFPIPVIAITGSNGKTTTKEMVAQVVAQRWKTLKTEGNLNN
                     RIGVPQTLFQLAPRHQAAVIEMGVDQQGQTTRLCEIARPTIGVITNIGPDHLEFFGSM
                     EGSAQAKAELLDHLPQDGAVVLNADDEYFDYLAARAQCRVVAFGVSPKAAIRAANIRV
                     DEKGGTVFGLVVPGKSRQTEIRIRTQGQHNVSNALAAAAVGYALGLSGTAIAEGLAKF
                     RPAAMRSQISMSHGVQVINDCYNANPASMKAAVQLLAELGRGKRSIAALGDMLELGTD
                     SKRMHREVGAFLAAQGIGHLLACGVLGRELAEGARQAGMASDSITELPDAAAAAVALA
                     RMVRQGDVVLVKASRGMRMEQVVEAVTGMRRVARKAC"
     gene            complement(3011..>3682)
                     /gene="murE"
                     /locus_tag="PKK81_06960"
     CDS             complement(3011..>3682)
                     /gene="murE"
                     /locus_tag="PKK81_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HNV32650.1"
                     /translation="SPAGTSFTLRSPVGTCPIKSRLVGEHNVSNLLAAIGVVLHEGLT
                     LEQVRSAVETVTNVPGRFELVEAGQDFSVVVDYAHTEDALVRLLTAAQALRTGRIITV
                     FGCGGDRDRTKRPKMGRAAVQYSDVVILTSDNPRTEDPAAILREVEVGVKEALADRGH
                     VRYRMVADRREAIEAAIREAKTGDMVLIAGKGHEDYQIVGTTKHHFDDREVARDTIGA
                     LRSGA"
     assembly_gap    3683..3782
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3783..4019)
                     /locus_tag="PKK81_06965"
     CDS             complement(<3783..4019)
                     /locus_tag="PKK81_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNV32651.1"
                     /translation="MTLDELISPIHGRLGVLERSGDQRVTIADLTDDSRKVGPGSLFV
                     AVQGERVDGHQFLDPVVAAGAAAVVVQRSGDVGAI"
     gene            complement(4021..5739)
                     /locus_tag="PKK81_06970"
     CDS             complement(4021..5739)
                     /locus_tag="PKK81_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNV32652.1"
                     /translation="MASTPFRSRRIVVACGLVVAFMLVIVRLVNLQVMQSAELTVKAD
                     RQHQKNVTLEGARGTIYDRNSKVLAMNMDVPSVFGVPASLGNPGATARNLSPILHVKA
                     TELEKKLKQEKHFVWLARKLEPEQGRRLERLALDGVGVVMEGRRFYPKGPLLSHVLGF
                     AGMDDRGLEGVELRYEQYLRGEKRAVVLQRDALGRAVFPKGLNEEGAAAGHSLTLTVD
                     EVIQYIAEKELDEAVSRSNAKSGTIIVMDPKTGAVLAMAVSPRFDPNTVGALAPDRWR
                     NRALTDTYEPGSTMKTVIAAAALEEKVMTPGSMIYGENGQLSIANTVIHDHEKTGWMT
                     FAQMIQKSSNIGAAKVGMALGEWRVFDYLKDFGFGDKTGIDLPGEAVGLLRGPRQWGK
                     RSLASISMGQEVGVTPLQMVTAVSAIANGGVLMKPFVVSEIRNAKGQLVAQTMPQAKR
                     RVISADTARTLTTLMEGVVTNGTGGKAAIPGFRVAGKTGTAQKVDPRTGAYSSTLLVG
                     SFLGYVPAEDPRLAMIVVIDEPRGEGWGGVVAAPVFRRVGEQVLNYLGVAVDEPVKLA
                     MAGLES"
     gene            complement(5739..6071)
                     /locus_tag="PKK81_06975"
     CDS             complement(5739..6071)
                     /locus_tag="PKK81_06975"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNV32653.1"
                     /translation="MKAVAFAAVTSLVLLFVWERVDIVRIGYHIERLKAQKVLLERER
                     DELRVKISGLTAPERIARLASDKLGMMQPEKGQVVVINIEPEAPANPVVAEGEVRIAK
                     NIVTRRAR"
     gene            complement(6068..6997)
                     /gene="rsmH"
                     /locus_tag="PKK81_06980"
     CDS             complement(6068..6997)
                     /gene="rsmH"
                     /locus_tag="PKK81_06980"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNV32654.1"
                     /translation="MEIGSARDSHEPVLVEEILFWLQCKPGGVYVDCTLGYAGLAARL
                     LDRTAPDGILVGIDRDAAALAESQMRLREVAHRVHFRHGNFCDLKALIMGSGVSQVDG
                     VIFDLGVSSPQLDRAERGFSFREDGPLDMRMDQREGRTAADLVRDLPETELADLIYQL
                     GEERYSRRIARAIVQARMQGVIGTTWQLAAVVERSVPASYRHGRIHCATRTFQALRIA
                     VNRELDVLEPALRDAVDILAPGGRVCAVSFHSLEDRIVKHTFRSLANGPEASVRVLTK
                     KPVIASESERSRNPRSRSAKLRVVERISKEYVK"
     gene            complement(7303..8595)
                     /gene="asnS"
                     /locus_tag="PKK81_06985"
     CDS             complement(7303..8595)
                     /gene="asnS"
                     /locus_tag="PKK81_06985"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNV32655.1"
                     /translation="MPVMYIDEAAAHAGQDVTIRGWLRSRRDKGKLHFLIVRDGTGDI
                     QAVVSKATVGDAQFARSAELTQESSLVLTGTLRQDARAPGGYELDVLRLEVLQVAEPF
                     PIQPKEHGTGFLMEHRHLWLRSSRQHAVLRIRHEIIRACRNFFDDRGFTLVDAPIFTP
                     NACEGTTTLFQTRYFDETAYLTQSGQLYSEATAAAFGKVYCFGPTFRAEKSKTRRHLM
                     EFWMVEPEVAFAQLPDMMDLAEQFLSHIVAAVLKSRRAELLLLERDPAKLEHVTAPFP
                     RISYEEAIDILQRRGNPAKPGDDFGADDETILSNEFDRPVIVHRYPAALKAFYMETDP
                     ERPDLALCMDVLAPEGYGEIIGGGQRIHSHEKLLGRIQEHHLPVEAFQWYLDLRRFGS
                     VPHAGFGMGIERAVAWICGLDHVRETIPFPRMLYRLYP"
     gene            8731..9825
                     /locus_tag="PKK81_06990"
     CDS             8731..9825
                     /locus_tag="PKK81_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32656.1"
                     /translation="MTHTTLLHHPRSRTPLLAALLLMGLLPSALAGEFRTDNPLNRPG
                     KIYWCPDRTADKRITATPDAGCTALYDEERDAKKPEKPDPPDHDASTPKEPLKIVDIQ
                     NEASKFTREYREFLECCAGEFDSVERIDELLDQSNHILMSVQQKGIYNSAGFGVGSGA
                     GGLGGGPGQSPKLGTFARQWTLSEIVGTVAIARDDLMKIKTRIKALGERFEKLDSLDY
                     EQRGREARRIDEEREAIAHDVRAKRPPASAPTGMDIQDTTIPTRIGGDIENTHLNRSL
                     NPSFGADIGATVSPYSNVNESLRPRRGEDLKDSNLPDRVGTHTQDTTLPNAFGFDINS
                     AENGERSSSVQTRGVGAEIGDSSLNNQSRR"
     gene            complement(9853..>10770)
                     /locus_tag="PKK81_06995"
     CDS             complement(9853..>10770)
                     /locus_tag="PKK81_06995"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32657.1"
                     /translation="DILYIATNDYIYKSRDGGKTWSNLSKGMSHSRVIAMAIDPAYPA
                     TIYAGTKGDAVYKSYDGGQRWTSIRGGLDDATISSVVNQFVFDPADDTHIFLATTMGV
                     FESRNAGERWTKRMDGMKEVLMVVTLGMDPTRSSVLYAGTSGGVYKSVNQGAYWQQVN
                     HGLVPEGMVKTSRALSVTSVQVDPFTTETVYAATLAGLYKTTDGAASWVRIGESLQDQ
                     MIVSMVLDRARPGVMYVAARDGIHRSDDGGKSWVALNEGLATTNIRSLAQSALDPQLF
                     YAGTNGSGLYRSTDGGAHWEALPSVFPKE"
     assembly_gap    10771..10870
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10871..11346)
                     /locus_tag="PKK81_07000"
     CDS             complement(<10871..11346)
                     /locus_tag="PKK81_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNV32658.1"
                     /translation="MADQSSFDVVSEVNMQEMKNVVDQATKEIKQRFDFKDSKTELTL
                     KEKEKELVVLSDDEYKLNAVIEIIKTKCVKRGVSLKAFEYGKIEEALGATVRQVIKIQ
                     SGISSEKAKEITKAVKESKIKVQAQIQGEQVRVISKSKDDLQTAIAFLKGKDFGIDL"
     gene            complement(11385..>13133)
                     /locus_tag="PKK81_07005"
     CDS             complement(11385..>13133)
                     /locus_tag="PKK81_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNV32659.1"
                     /translation="VVLFGHHFAAIAGAGPLLGPVLAAQFGFLPGFLWLVIGAVLAGA
                     VQDFIILVASMRRNGRSLPEIAHDELGSITGTATAVAVLFIVVVALAGLGFAVVNALY
                     HNAWGTFTIAMTIPIGFIMGFYLQKFRPGAVAEVSVIGVVLLVAAVLFGRVVGQSSLA
                     WLFEFEKPALVWLLAGYGFLASVLPGWMLLVPRGYLSTFMKLGVVFLLGFGVILMAPT
                     IEMPRVTAFANGGGPIIPGTLFPFLFITIACGAVSGFHALVSSGTTPKMIEQESQAVV
                     GYAAMLLESFVGVMALIAASVLIPGDYLAINTMLPAETLTAMGFAPSRIAELSQLVEV
                     NVAGRPGGAVSLAVGMASIFSALPGMAGLMAYWYQFALVFEALFILTTIDTGTRVARY
                     LIQEMAGRVYAPFRQMNWWPGVLLSSAFVVGSWAYLIGTGSISTIWPMFGAANQLLGT
                     LALCIGTTVLIKMWKSPYLWVTALPMLFVGGITLAGSYEMFWMFLAKAATLAAGQAFA
                     LYLDAVLVAVVAMLGLVVLSDSMKQWYGYVILKRPFSSSEVMARAGGGSAGRVQTAIH
                     RHDERVFKLPPGGGCC"
BASE COUNT         2797 a         4375 c         3358 g         2403 t
ORIGIN      
        1 ttgccgatca gcgtcaccgt cgtgctcttc ccgttggtcc cggtcacggc caccaccggc
       61 gccgtcaaaa agcgggacgc caattccagc tccccgatga cccggacccc ccgggccctc
      121 acccgattga gcgcatccag ttgagtcggc acacccgggc tgatcacgac aagatcagcc
      181 ccttcgagtg cggactcgta ctgcaccccc accttcaccg cgatgctcga ccggtccacc
      241 tgcgacagga tggcggtcag ttcctgcgcc tccttccggt cggccagggt gacccgggcc
      301 ccgagatggt tcaacaggcg tgccgcgccc actccgcttc gggctagtcc gacaaccgtg
      361 acttggagat ctttcacgtt cactgctgtt cgcctaccgt aatttgagcg tgctcaagct
      421 gagcaacgcc agcaaaatgg caatgatcca cagacgcacc acgaccttcg gctcgtccca
      481 ccccttcatc tcgaagtgat ggtgaatggg cgccatattg aaaatgcgtt tcccccggag
      541 cttataggat cccacctgca ggatgaccga caacgcttcg atgacgaata cgccgccgac
      601 caggagcaac agcagttcgt gcttgctgat cacggccaca gtaccgaggg ctgcccccaa
      661 cgggagcgag cccacgtcgc ccatgaagac ggaggctgga taggtgttga accacaaaaa
      721 gcccagactg gatcccagga tggctccggt gaagatggcg atttctcccg cgccttcgat
      781 gtaggggata agcaaatatt cggccatcac acggttcccg gtcacatagg ccacgatcgt
      841 atacgccagc gaggcgatca tgaccggacc gatcgccagc ccatcgagcc cgtccgtcaa
      901 attgacggcg ttggagctgc cgacaatgac aagaatgacg aacacgatgt agaaccagcc
      961 gaggtccggc gtgaaaaatt taaagaacgg cacgctgagt ttcgtcgtat agctgggcaa
     1021 cgtgtagaga aagacgccga tggtcagcgc gaccagaatc tggcaggtga atttttgtgc
     1081 cgccgagagg cctttcgatt ggcgcttgat gaacttgaga tagtcatccg cgaacccgat
     1141 cgcgccgaag cccaccgtcg ccaccacgac cagccacacg tatcggttgg tcatgtcggc
     1201 ccacagcaac gtggacaaca ccaccgcgaa gataatgagt atcccaccca tggtcggcgt
     1261 gccgcttttg gccaaatgcc gcttcggccc atcgtcgcga atctgctgac cgagcttaat
     1321 ctcctgcaat ttgcggatga cccagggagc catcacaaac gcgatcaaaa acgccgtgac
     1381 cgccgcatag ataatccgga agctctggta ccgaaagacg ttcaggaatg aaaactgtgt
     1441 gtgaaggggg tatagccagt tgtataacat cctacggcat ctttgcccgc catggatcag
     1501 cgggccctaa agttcctctg tatgtgcctg ccgactagca ggcctttcgc gcgacccgtc
     1561 gcattcccgt cacggcttcc acgacctgct ccatgcgcat cccgcgcgac gccttgacca
     1621 gcaccacatc gccttgccgg accatccgtg ccagggcaac ggctgccgcc gccgcatcgg
     1681 gcaactccgt gatgctgtcg gacgccatcc cggcctgccg ggctccttcg gccaactctc
     1741 gtcccaatac accacaggcg agcagatgtc cgatgccttg cgcggccaga aacgccccga
     1801 cctcccggtg catccgcttg ctgtccgtgc ccagctccag catgtcgccc agcgccgcga
     1861 tcgaccgttt tccccgaccg agctccgcca gcagttgaac ggccgctttc atcgaggccg
     1921 gattggcgtt gtagcaatcg ttaatcacct gcaccccatg ggacatgctg atctgcgatc
     1981 gcatcgcggc cggccgaaat ttggccaaac cttcggcaat ggccgttccc gataagccca
     2041 acgcataccc gaccgcagcc gcggcgaggg cattgctcac attgtgttgc ccctgcgtcc
     2101 ggatgcgaat ttcagtctga cgactctttc ccggcaccac caggccgaac accgttccac
     2161 ccttttcatc gacccgaata ttggcggcgc ggattgctgc cttgggagac accccgaacg
     2221 ccacgacccg gcactgcgcc cgggccgcga gataatcaaa atattcatca tcggcgttca
     2281 acaccaccgc cccgtcctgc ggcaaatggt cgagcaactc ggccttggcc tgcgcagacc
     2341 cttccatact gccgaaaaac tccaaatggt ccgggccgat gttggtaatc acgccaatcg
     2401 tcggtctcgc gatttcgcac agccgggtgg tctgcccctg ctgatccacc cccatttcaa
     2461 tgacggcggc ctgatggcgc ggcgccaact gaaacagagt ctgcgggact ccgatccgat
     2521 tattgaggtt gccctcggtc ttgagcgttt tccagcgctg tgcaaccacc tgggcgacca
     2581 tttccttcgt cgtcgtcttg ccgttgctcc ccgtgatggc aatgaccgga atgggaaacc
     2641 ggtttcgata gtgcgtggcc agccgttgat aggcctcgag cgtatctctc accccgagca
     2701 gcatgggcac gccggtacgt ttcggcgccg gcggcaggcg atagtcctct tgcacgatgg
     2761 cacagaccgc cccctgggcc aacaccttcg gcacgaaggt atgcgcgtca aaccgctcgc
     2821 cctggaaggc gatgaagaga tcgcctttgc gcaccaatcg cgaatcggtc accacacgac
     2881 gaatgcgttg tttcaagtcc tgaggcgtga cgccggccgg cggtctgaca ctcaagacct
     2941 cacagatttc ctcgaccgta aacagcgcca tcacaccgcg attcccatgc tcccgcatgc
     3001 gttccctcgc tcaggctccg gatcggagcg cgccgatcgt gtcgcgcgcc acttcccgat
     3061 cgtcgaagtg atgtttcgtg gttcccacga tctgataatc ttcgtgacct ttccccgcga
     3121 tcagcaccat gtccccggtc ttcgcctcac gaatggccgc ctcgatggcc tcccgacggt
     3181 ctgcaaccat gcgatagcga acatgacccc ggtccgccag cgcctccttg accccgacct
     3241 cgacctcgcg caggatcgca gcaggatctt cggtacgcgg gttatccgat gtcagaatca
     3301 ccacatcact atactgcacg gctgcacgtc ccattttggg gcgtttggtc cggtcacggt
     3361 ccccgccaca cccgaagaca gtgatgatgc gccccgtgcg aagcgcctgg gctgcggtca
     3421 acaaccgaac cagcgcgtct tcggtatgcg catagtccac caccacggaa aagtcctgac
     3481 cggcttcgac cagttcgaac cgccccggca cgttcgtgac cgtctcgaca gcggaccgta
     3541 cctgctccag cgtcagccct tcatgcaaca ccactccgat ggcagccagg aggttcgaca
     3601 cattatgctc tccgaccaga cggctcttga tcgggcaggt cccgacggga ctgcgcaacg
     3661 taaaactggt gccggccggc gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnaatcgcac cgacgtcgcc gcttcgctgc accacgacgg ccgccgcccc cgccgcgacc
     3841 acagggtcga gaaactgatg gccatcgacc cgctctccct gcacagccac gaagagggac
     3901 ccgggtccca ccttccgcga atcgtcagtc agatcggcaa tggtcacgcg ctggtctccg
     3961 cttcgttcca gcactccgag acgcccatga atcggactga tcaattcatc gagtgtcata
     4021 tcaggactca aggccggcca tggccaactt gaccggctca tccactgcca cgcccagata
     4081 gttgagtacc tgctcaccga cccgacggaa taccggcgcg gccacgacgc caccccagcc
     4141 ttcaccgcgc ggctcgtcga tcacgacgat catcgccagt cgaggatctt ccgccggcac
     4201 atacccgaga aacgacccga ccagcagcgt ggacgaatag gcgccggtgc ggggatcaac
     4261 cttttgagcc gtaccggtct tgccggccac ccggaagccc gggatcgccg ctttgccgcc
     4321 ggtgccgttc gtgaccactc cctccatcag cgtcgtcagc gtcctcgccg tatcggcaga
     4381 aatgactcgc cgcttcgctt gcggcatggt ttgcgccacc aattgtccct tggcgttacg
     4441 gatctcggag accacgaagg gcttcatcaa cacgcccccg ttcgcaatgg ctgagacggc
     4501 cgtgaccatc tgcagcggcg tcacgccgac ctcttgcccc atcgaaatcg aagccagaga
     4561 acgcttgccc cactggcgcg gtccccgcag caaccccacg gcttccccgg gcaaatcgat
     4621 gccggttttg tcgccgaacc cgaagtcttt gagataatcg aatacccgcc actcacccaa
     4681 ggccatgccg accttcgccg cgccgatatt gctcgacttc tgaatcatct gcgcgaaggt
     4741 catccagccg gttttttcgt gatcgtgaat cacggtgttg gcgatcgaga gctgcccgtt
     4801 ctctccatag atcatgcttc cgggcgtcat caccttctct tcgagcgctg ccgccgcaat
     4861 caccgtcttc atggtggacc cgggctcata ggtatccgtc aaggcgcgat tgcgccaccg
     4921 gtccggggcc agcgctccga ccgtgttggg gtcgaatcgt ggactgaccg ccatggccaa
     4981 cacggccccg gttttcgggt ccatgacaat gatcgtgcct gacttggcat tcgatcgact
     5041 cacggcctca tccagctctt tctcagcgat gtactgaatc acttcgtcga cggtgagggt
     5101 cagactgtgc ccggctgccg cgccctcctc attgagtcct ttcgggaata cggcgcgtcc
     5161 caacgcatca cgctgcaaca ccaccgcccg cttttcacca cgcagatatt gctcgtaacg
     5221 cagctccacg ccttccaacc cacgatcgtc catgccggca aagcccagca cgtgcgacaa
     5281 cagcggcccc ttgggataga agcggcgccc ttccatcaca accccgactc catcgagagc
     5341 aagtcgttca agccgccgcc cctgctccgg ctcaagcttt cgcgccagcc acacaaaatg
     5401 cttttcctgc ttcagcttct tttcgagttc cgtcgccttg acgtgcaaga tcggcgagag
     5461 attgcgcgcc gtcgcaccgg gattcccgag ggaagccgga actccgaata cggacggcac
     5521 gtccatgttc atcgccagca ccttgctgtt tcgatcgtag atcgtccccc gcgcgccttc
     5581 cagcgtgaca tttttctgat gctgccgatc ggccttcacg gtcagctccg ccgactgcat
     5641 cacctggaga ttcacgagac ggacaatgac cagcatgaac gcgaccacca gcccgcaggc
     5701 caccacaata cggcgactgc gaaaaggggt cgaagccact atcgcgctct ccgcgttacg
     5761 atgtttttgg cgatccgcac ctcaccctcc gcaacgaccg gattggccgg cgcttccggc
     5821 tcgatgttga tgacgacgac ctggcccttc tccggctgca tcatccccaa cttgtcgctg
     5881 gccagacgcg caatccgctc aggtgccgtg aggccggaga tcttcacgcg caattcatcg
     5941 cgctcccgct caaggaggac cttctgcgcc ttcagccgtt cgatgtggta gccgatgcga
     6001 acgatgtcca cccgctccca cacaaagagc aacaccagcg acgtgaccgc ggcaaacgcg
     6061 acggctttca tttcacatac tccttggaga tccgctccac gactcgcaac tttgcgctcc
     6121 gtgaccgcgg gtttcggcta cgctcgcttt ccgaggcgat gaccggtttt ttcgtcaaca
     6181 ccctgaccga cgcttcgggg ccgttcgcca gcgaccgaaa cgtatgcttc acgatcctgt
     6241 cttcgagcga atgaaacgag acggcacaga cacgcccgcc cggcgccaga atgtccaccg
     6301 catcccggag cgccggctcc aacacgtcca actcacgatt cacggcaatc cgcaacgcct
     6361 gaaaggtgcg cgtggcgcaa tgaattcgtc cgtgccgata cgaagccggc accgagcgtt
     6421 ccaccactgc cgccaactgc caggttgttc cgatcacccc ctgcatccgg gcctgcacga
     6481 tcgcgcgggc aattctgcgc gagtaccgct cctcaccgag ctgatagatc agatcggcca
     6541 actcggtctc gggcagatcg cgaaccaggt ctgcagccgt gcgtccttcg cgctgatcca
     6601 tgcgcatatc cagcgggccg tcttcccgaa aactgaaacc gcgctccgca cgatcgagct
     6661 gaggcgagga cacgcccaga tcgaaaatca cgccatccac ctgcgacacc ccgctcccca
     6721 tgatcaacgc tttcaggtcg cagaaatttc catgtcggaa atgcacccga tgcgcaacct
     6781 ctcgcagacg catttgcgac tccgcgagtg ccgcggcatc gcgatcaatc cccacaagga
     6841 tgccatctgg agcggtgcga tcgaggagac gagcagcgag ccctgcatat ccaagagtgc
     6901 agtccacgta aacaccacct ggtttacact gcaaccagaa caagatctct tcgactaaaa
     6961 ccggctcatg ggaatcgcga gcagaaccta tttccatttc cacccacttc accccactca
     7021 ttgggtggag tatacaccca accaaatgac tgtcaacaga attaacccct atttatcaag
     7081 aatattgttc agtttagaca catgtctaat ttggccggaa tttgcaggaa agttatccac
     7141 atttgtggac aagtctgtgg gaaactcgcc cacctgacaa ttatcttcgg gcaagtttct
     7201 gtcaggggaa aatggggatc atcaaaaaat cagatggatg aaatacctgc atttcggtgg
     7261 tcgctcgcgc agaaatgcag gcagaaacgg aaggactcgc cgctatggat aaagtcgata
     7321 caacatccgg ggaaagggaa tcgtttcccg cacgtgatcg agcccacaaa tccaagccac
     7381 ggcacgctca atacccattc caaaccccgc atgcggaacc gagccgaacc ggcgaaggtc
     7441 gagataccat tgaaacgcct caaccggtag atggtgctcc tgaatgcgtc ccaaaagttt
     7501 ttcgtgagaa tggatgcgtt ggccaccgcc gatgatttct ccatagcctt ccggcgccag
     7561 cacatccata cagagggcca gatcaggccg ctccggatcc gtctccatat aaaatgcttt
     7621 caaggccgca gggtatcgat gcacgatgac cggtcgatcg aattcattgg acagaatcgt
     7681 ctcgtcatcg gcaccaaaat cgtcaccggg cttcgcggga tttcctctcc gctgcagaat
     7741 gtcgatggcc tcttcgtagc taatgcgcgg aaacggcgcc gtcacatgct ccagcttcgc
     7801 cggatcccgt tcaagcagca gcagctcggc gcgacgactc ttcagcaccg ccgccacaat
     7861 atgagacagg aactgttcag ccagatccat catatcgggc aattgcgcaa aggccacctc
     7921 cggctccacc atccaaaatt ccatcaaatg ccggcgcgtc ttggacttct ccgcgcgaaa
     7981 ggtcggaccg aagcaataca cctttccaaa cgccgcggct gtggcctcac tatagagttg
     8041 cccgctctga gtcaaatagg ccgtctcatc aaagtaccgc gtttgaaata aggtggtcgt
     8101 gccctcacag gcattcggtg tgaaaatcgg cgcatcgacc agcgtaaaac cacgatcgtc
     8161 aaaaaagttc cggcaggctc gaataatttc atggcgaatg cgcagcacgg catgctggcg
     8221 gctggagcgc aaccagagat ggcgatgctc catcaagaat cccgtgccgt gttctttcgg
     8281 ttggatggga aaaggctccg ctacctgcaa cacttcaaga cgcagcacgt cgagctcata
     8341 cccgcccggc gcacgcgcat cctgccgtaa cgtgccggtg aggacgagac tggactcctg
     8401 ggtgagttcc gccgatcgag caaactgtgc atcacccacc gtcgctttgc tcacgaccgc
     8461 ctgaatgtcc ccggttccat ctcgcacgat cagaaaatgg agcttgccct tgtctcgacg
     8521 actgcggagc cacccccgaa tcgtcacatc ctgtccggca tgggcggccg cttcatcgat
     8581 atacatcaca ggcatgggtc atcctattca aatagaggtg cgccggagag gtctatggcc
     8641 ggagggtaac gaacagtcgc gggcttttca agcgcatcac aaatcccccc tttcgttgac
     8701 acaatgtcat gccgggggca tcatcacatc atgacgcaca ccacattgct ccaccatccc
     8761 cgatcacgta caccgctgct cgccgcgctt ctcctgatgg gcctgttgcc ctccgcactg
     8821 gcaggtgaat tccgaaccga caacccgtta aaccggcccg gaaaaatcta ttggtgcccg
     8881 gaccgcacgg cagacaagcg gatcaccgcc actcccgatg caggctgtac ggccctgtac
     8941 gatgaggaac gcgacgcgaa gaaaccggag aagccggacc cgcctgatca tgacgcctcc
     9001 actccgaagg agccgctcaa gatcgttgac atccagaatg aagcgtcgaa gtttacccgc
     9061 gaatatcgag aatttctgga atgctgcgcc ggcgagttcg attccgtcga acgcatcgac
     9121 gaacttctgg accagtcgaa ccacattctg atgtcggtgc aacaaaaagg catttacaac
     9181 agtgcgggct tcggcgtggg aagcggggca ggagggctcg gcggcggccc cggtcaatcg
     9241 ccgaaactcg gcacctttgc ccgccagtgg acactcagcg agatcgtcgg aaccgtcgcc
     9301 atcgctcggg acgacctcat gaaaatcaag acgcgcatca aggcccttgg agagcgattc
     9361 gagaagctcg attcattgga ctatgagcag cgcgggagag aagcgcggcg catcgacgag
     9421 gaacgcgaag ccattgctca tgacgttcgg gccaagaggc caccggcctc agcaccgaca
     9481 ggcatggaca tccaggacac caccatcccg accagaatcg gcggagacat cgagaacacc
     9541 catttgaatc gatccctcaa tccaagcttc ggcgccgaca tcggagccac ggtgtcgccg
     9601 tacagcaacg tcaacgaatc cctgcgaccc cgcagaggag aagacctcaa agacagtaac
     9661 ttgccggacc gggtgggcac tcacacccag gatactaccc tgccgaatgc attcggcttt
     9721 gacatcaatt cagccgaaaa cggcgaacgc tcgtcgtcgg tgcagacgcg gggcgtcggc
     9781 gccgaaatcg gcgactcctc cctcaataac cagtcccgcc ggtagactcg cgccggccgg
     9841 atctacaccc ggttattcct tgggaaagac cgatggaagc gcctcccaat gcgcccctcc
     9901 gtccgtactc cggtataatc cactgccgtt cgtgccggcg tagaacagct gtggatccag
     9961 cgcgctctgc gccagcgacc ggatattggt ggtcgccaac ccctcgttca atgccaccca
    10021 gctctttccg ccatcgtcgc tccggtggat gccatcccgc gcagccacat acatcactcc
    10081 aggccgcgca cgatcgagta ccatcgacac gatcatctga tcctgcaacg attcgccgat
    10141 gcgtacccag gaagcggcgc cgtcggtggt tttatagaga ccggccaggg tcgccgcata
    10201 gaccgtctcc gtcgtgaatg ggtcgacctg caccgacgtg acgctcagtg cgcgcgatgt
    10261 tttcaccatc ccctccggca ccaaaccatg attcacctgc tgccaatacg ccccctgatt
    10321 caccgacttg tagacaccgc cgctggtgcc ggcatagagc acggatgatc gcgtgggatc
    10381 catgcccagc gtgaccacca tcaacacttc cttcattccg tccatccgct tcgtccaccg
    10441 ctcaccggca ttcctcgatt cgaacacccc catggtcgta gccagaaaga tatgagtatc
    10501 gtctgcgggg tcgaagacaa attggttcac gaccgaggaa atcgtggcgt cgtcgagccc
    10561 gccacgtatg gacgtccaac gctgcccccc gtcgtagctc ttgtatacgg catcgccttt
    10621 tgtcccggca taaatcgtcg caggataagc ggggtcaatc gccatcgcaa tcacgcgcga
    10681 gtggctcatc cctttcgaga gattgctcca ggtcttgccg ccgtcacgcg acttgtaaat
    10741 gtagtcgttc gtggcgatgt agagaatgtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn agatcaatcc caaaatcctt ccccttgagg aaggcaatcg ccgtctgcag
    10921 atcatcttta ctcttgctga tcacgcgcac ctgctcgcct tgaatttgcg cctgcacctt
    10981 tatcttggac tctttcacgg ccttggtgat ttccttggcc ttctccgatg agataccgct
    11041 ttgaatcttg atcacctgcc ggactgtagc gccgagcgcc tcctcgattt tgccgtactc
    11101 gaacgccttc aacgagaccc cgcgcttcac acacttggtc ttaataattt caatgaccgc
    11161 attgagcttg tactcgtcgt cggagagcac caccagttcc ttttctttct ccttcagcgt
    11221 cagctcggtc tttgaatctt tgaaatcgaa gcgttgcttg atttccttcg tcgcctgatc
    11281 gacgacgttc ttcatctcct gcatattcac ttccgacacc acatcaaacg atgattggtc
    11341 agccacgatg ccctcctcct gctgtttcgt caggtcttcc gcccctaaca gcatcctccg
    11401 ccaggcggca atttaaacac gcgctcatca tgccggtgaa tcgccgtctg cacgcgaccg
    11461 gccgatccac cgccggccct ggccatcact tcactgctgc tgaacggccg cttcaaaatc
    11521 acatacccat accactgctt catgctgtcg ctcaagacga ccaggcccaa catcgcgacc
    11581 accgccacca gcacggcatc gagatacaat gcaaaggcct gccccgccgc aagtgtcgcc
    11641 gccttcgcca aaaacatcca aaacatctca tacgagccag ccagcgtaat gcctcccacg
    11701 aacaacatcg gcagcgccgt cacccagaga tagggcgact tccacatttt gatcaacacc
    11761 gtcgtgccga tacagagcgc gagcgtgccc agcaattgat tggctgcgcc gaacatgggc
    11821 cagatggtcg aaatgctgcc cgtcccgatc agataggccc acgaccccac cacaaaggcg
    11881 ctgctcaaca acacgcccgg ccaccagttc atctgtcgaa acggcgcata cacccgcccg
    11941 gccatctcct gaatgagata cctggccaca cgggtccccg tatcgatggt cgtcagaatg
    12001 aacagcgcct cgaacacgag ggcgaattga taccaatagg ccatcagccc ggccatgccg
    12061 gggagcgccg aaaagatgga ggccatgccc actgccaacg acaccgcccc tcccggccgc
    12121 cccgccacat tcacttcaac cagttgcgac aactccgcga tgcgggaagg cgcaaaaccc
    12181 atcgccgtca acgtctcggc cggcaacatg gtgttgatcg ccaggtaatc gccggggatc
    12241 agcaccgacg ccgcaatcaa cgccatcacg ccgacgaagc tttccagcaa catcgccgca
    12301 tagcccacca ctgcctgcga ctcctgctcg atcatcttcg gtgtcgtgcc ggaggacacc
    12361 agcgcatgaa agccggacac ggccccgcag gcaatcgtaa taaagagaaa cggaaacaac
    12421 gtgccgggaa taatcggccc gccgccattg gcgaaggcgg tcacacgcgg catctcgatc
    12481 gtcggcgcca tgaggatcac cccgaagccg agcaaaaaca ccacgccgag cttcatgaag
    12541 gtggaaagat agccgcgcgg caccaacagc atccagccgg gcaacaccga agccaggaaa
    12601 ccatacccgg ccagcaacca gaccagcgcc ggcttctcga actcgaagag ccaggcaaga
    12661 gacgactgtc cgaccacacg cccgaagagc accgccgcca ccaacagcac caccccgatc
    12721 accgacactt cagccaccgc gccggggcgg aacttctgga gatagaaacc catgatgaac
    12781 ccgatgggaa tcgtcatggc gatggtgaag gtaccccagg cattgtggta cagcgcattc
    12841 accaccgcga aacccagtcc cgccagcgcc acgacgacaa taaagagtac cgccaccgcc
    12901 gtcgccgtcc cggtgatgga gcccagttcg tcatgggcga tttcaggcaa tgaacggccg
    12961 ttgcgtcgca tcgaagcgac gaggatgatg aagtcctgca ccgcccccgc cagcaccgcg
    13021 ccgatcacga gccagagaaa accgggaaga aagccgaact gcgccgccaa caccggtccc
    13081 agcaacggcc cggctccggc aatcgccgca aagtgatggc cgaacaagac gac
//
LOCUS       DAOQRM010000066        13002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3881_length_13002_cov_17.403955, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000066 DAOQRM010000000
VERSION     DAOQRM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13002
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3881_length_13002_cov_17.403955"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..3434)
                     /locus_tag="PKK81_07010"
     CDS             complement(138..3434)
                     /locus_tag="PKK81_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32660.1"
                     /translation="MAFKGDDSTFSLATTVTLVLAVAAMVFVKEPLKSSRPTGNGVGL
                     EGAASELKARARLWEDPFAAVQKDQASRKQVSVTLQKAGKVTLSVTAVGRNEEEGLSK
                     LQAKINETAKQNGIVTALVVMTGGGSSVEDSEARIRDRYAVGAALEVGCFVPEQTQSL
                     SYFTWSFGVGKVEPKVPVTTPYEWYKRSVIASCRAFDEEMSKEKARHVLVLWVAAEET
                     EQTIFARVKQLLSKFRPPNVESSIPAKLVGPRSSSEFRSMLQEIEEESKSSDRAKGRQ
                     KAGDSPPKVQEPIALYSPWATAMPGLLSYGLNSGSQKNGVGCQSYGACKKVFTELLSD
                     AGLLLKYSIDSDQVLFESLFEELNRRRVTVGADSIALIGEWDSFYARSLPLTFSAAAC
                     RYITDEKVAKAHPLSDALTGQLKGKCATTEQGIDQFKTGAISPRTLNIRQYSYLSGLD
                     GEASVDQGSPSRLKGDDKDKEKGEAGKAKLRDIASYERPEGPSQLDYVRRLVARIKTE
                     DQENDKAGTRGEAEQQGKKVKAVGILGRDAYDALLILQAVREQFPNALFFATDLDARY
                     SHEDELKWTRNLIVVSHFGLRLQPSLQQAIPPFRHSLQTSTFLAVLRAIDHVGYRLDQ
                     SAGPNAQLAGYVMNGGQQQQIEYSTVIRPRLFEIGRHGAVDLSVDNSTDKVRSVHPAR
                     QDVESWAVTFRMPERIGPVWIAVTVLGFLTAWGYGRFWNWLTARDERDRSSRNFKRVL
                     RPAWVPLLILGVVLLWCKLRHFSYVEDEPFSWSDGVSVWPTELLRLFATLLSLSFLMK
                     ACADLAVNTEDLTKKFFPQGASAGVEEGRPRNLQGFWTNLDWMFHGSKRDRTGEARTL
                     WARYCRAHTWPQRTVRIALWLLLYLMMVLPVWRFMNDGEWRLYVPCRGEFSCHADAVL
                     TSLSVFSLIILNLAVLDAVILCTKWIQEMPSATGLDPIRQVRLIIERTRVVNRRILYP
                     FLSLFLLIGARSHYFDNWDFPPALILVLTVNSLVALASACMLYLAAVEAKRKVLAPLQ
                     QDLDRVLIQQECAEPAVDTGPSSDHLRQIISEIDAVQQGAFVPFYQQPVVQATLVAVL
                     AFLQYWYLGQ"
     gene            complement(3666..5141)
                     /locus_tag="PKK81_07015"
     CDS             complement(3666..5141)
                     /locus_tag="PKK81_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNV32661.1"
                     /translation="MTVHSLETLTFDNSYARLPEVFYAKVSPTPFSAAPFLISANRAA
                     MELLDLDPAEAARPEFAGVFGGSLLIPGMEPLAMLYSGHQFGVYVPQLGDGRAILLGE
                     VTNGRGERWDLHLKGAGMTPFSRDGDGRSVLRSAIREYLCCEAMHGLGIPTTRALCLV
                     GSDDKVYREQIETGATIVRMAPSHVRFGTFEIFYYRKQHEHLQRLADYTIEMHFPELA
                     QAVDKYARFFAEVVQRTAKLIAHWQAVGWSHGVLNTDNMSILGLTLDYGPYGFMDDYD
                     PGFICNHSDYNGRYAFNQQPYIGLWNLSCLAQTLLPFAPKEELKAALDGYQTSVDRHY
                     HNHMRAKLGLVEDRAEDEALLQELKSLMVGSRVDYTIFWRELSMFSSDAGAKNERLRE
                     HFLNPERFDTWAVQYRERLQSEQSRDEERRIRMDRVNPKYVLRNYLAQGAIEKAQQKD
                     YSEVERLLALLQQPYTEQPGMNSYAAAPPNWGKHLSVSCSS"
     gene            complement(5163..5588)
                     /locus_tag="PKK81_07020"
     CDS             complement(5163..5588)
                     /locus_tag="PKK81_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HNV32662.1"
                     /translation="MAVTIIGGALSRSTIQKDLLPVASKGEKEAAVRTAIIKFEQEFL
                     GRGPDEVRVFIVRDMLVVRLKGVLTPAERQLAKTAEGIDMVKRLRQNLIAQGRERLCE
                     QVMDLIGAKVTALFTDIDTVVGERIFVFTLESDIEANFR"
     gene            5931..7625
                     /locus_tag="PKK81_07025"
     CDS             5931..7625
                     /locus_tag="PKK81_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNV32663.1"
                     /translation="MSFLALVPLLPLLTAFIVMTGDRAEQDQNARAGLLPVAAAFLGS
                     LIALVAVTSGDPVTIQFYDTASVANLAFPIGFYIDRLSAVMMVLITGVTMLIYRYSMG
                     YMYQDRGYRRYLGMLGITTAVLLCMVSSANLVMLFVFWQILSWLLFLLAHNHGHAATL
                     SGATNTFTMLRLSDAAFLAGIVLAYSLYGTFEFQTLFARAADTPVTLSLWPDLGWEMN
                     GATAVTLLIFVGAMGKSAQFPIHTWLPRSLYAPTPIHALLHAGIINAGGFLLNRLAPL
                     YGLSPTTLHVVFVIGMLTAILGATMMLTQNDIKKTLGFSTIGQMGYMIMECGLGAFSL
                     AVFHLIAHGLFKGTVFLNCGNVIHKARQEPSFPPIDREAEESEFSNLTWSTGFLTTLL
                     LPLVILLVTHGVLRIPLIDSQGTVIFLFFIWVTSSQAILSLTRIRAVASWKVSAAMLV
                     TLVIVVFTYLFAVESFTHFLYPNPEVVASYFKAAALPGRLFDSLVVGTTVLTILSWVY
                     LYAHAHGRTVKIPGWIDVFLVRLYVLFMNRLYLDQLYLKFGRIVTLVAHHLEKRLS"
     gene            7681..8988
                     /locus_tag="PKK81_07030"
     CDS             7681..8988
                     /locus_tag="PKK81_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32664.1"
                     /translation="MIKTWMTPWLLMAVPFLGATLSLLLRTSLQRMKTSALLTTVASM
                     LVVIGTPWALGDTLAGVPFLCLLPLTAFLSLLGQPLHRDNTSAWTTTLVLLGLGLGIL
                     TAQAPAHDVMLLILPGLLCILLYRYQSGATPETWRGLSAYGLSMVSAALALVLPTPAS
                     TVALLVTCATLLPLLPVHSGFIATLTGLPGNLPAFLVFLLPTVGFHTLLMLLPNLTGT
                     TLHTVAILALAGAVYGSLRALIQARPLPRLSYAALAFFGLLWWYVADTGTAPMQATVY
                     LSAAGLAASGLLLAWYAIRARYGDIDLRALGGLAYPMPRFSTLLALLALAALGMPPFG
                     VFSGFLGMLLLPTFTPSGPFAVVMIVWLTASWYYTDFLQQLIFGRQRMDMRYDDLRQT
                     EFASLVLLLLLLLALGTAPSRFFDSYIPTPPATVAMQEGTWIR"
     gene            8976..12245
                     /locus_tag="PKK81_07035"
     CDS             8976..12245
                     /locus_tag="PKK81_07035"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNV32665.1"
                     /translation="MDPVIRADDPADLEARRMELRGTIRLASEVIAQYWPMRTFVHHN
                     PLHSLEYLPFTETVRRGHQFLGGNGYLPGDVYRRYLKSGRILVRHIDEALAPLALEQE
                     VDLGPCRIAHREVLRACLTHGLSTATDEPLDRLMEREPNEEQVEALAERLGSFSTPTV
                     AERMAATVRADSDALGHHLTLSNWCDQTLGTRIVELINSELIKWCEAFLDEGHATWPM
                     PGREQGLYAAWKQVAGKEWTTCGIADSRRKIAALPEHPEDAVLDCLDALAIPVTFRQD
                     YLSLQLAALPGWAGFIKWRAEETGYAWQQAYPVGLVKFLAVRLWYVRELVQKVCREEL
                     GIDGNYPAVMAYMAQQSHAYFMHKEWAAGRLPASMADRVARLHADTRHRQQGTPALAP
                     EWEPLTHHDQIEFGPRRERAAQRLMARRLLTLAQALEIVPALLMDGPLTALRTLLNWI
                     DAFPESAHGPVWLKAFEAGYQDHLFGMLLRAPVKPQPVSAEHHPPVRPHSQSVFCIDV
                     RSEPFRRHLESTGANDTYGFAGFFAVFIRYRAWGKEHETEQFPVIMRAKNEVREIPRS
                     YLDHYVSKHQSRAKLVHAGHTLLHDLKENVVTPYVMVESLGWFYALPMMGKTMWPALY
                     KRLTNWVRRLFVPPIATILTVDKLAPAETEEMMVSEQRALIWKALRDRLGLHGSRVEA
                     EFVEALRRRALDDDAPVEPFLSDAAKSVDLSADQLTTFLEELRRHYRINRRAASRQKE
                     RITRTGFTLEEQVLTVETALRMMGLVRNFARLVLFCAHGSTTENNPFESALDCGACGG
                     NEGKPNARVLAAMANRPPVRERLAKRGIEIPSDTHFLAGQVDTTTDEVHLFDLEDAPP
                     THRKDVARLYDDLREAAQLTSQERCSRFPDVRTVLPLNQASAHVAGRSADWSQVRPEW
                     GLSGNTTFIIGRRELTKGLNLAGRVFLHSYDYREDPTDRWLEVLLTAPQVVAQWINME
                     HYFSAVDNEVYGSGSKIYHNVVGRIGIMSGPWSDLRLGLARQTVMNDDLPYHEPMRLL
                     TLVETSRPRIEKLIARHEVLQHFYHNEWVHLAALDPEDGIWYRYMPSGVWRRVRNPSD
                     T"
     gene            12297..12638
                     /locus_tag="PKK81_07040"
     CDS             12297..12638
                     /locus_tag="PKK81_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNV32666.1"
                     /translation="MAALTLHPMKEIRVIVSGEHRPFVTELLDKVEATGYTIIGNISG
                     KGHHGLREAHFMFSEQESLVMIMAVVPEEKVEPLLAGLRPLFERHSGVMFVSDVAVSR
                     REYFGKKSAKS"
     gene            12755..>13002
                     /locus_tag="PKK81_07045"
     CDS             12755..>13002
                     /locus_tag="PKK81_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32667.1"
                     /translation="MKGLRFERLGRDRHYNIIFHIGSSYVPVSDETIEELKAQTLLSS
                     ERFLDLLLDKIGYTSYLKDQIRTELQASGDPMTQMTVL"
BASE COUNT         2479 a         4177 c         3687 g         2659 t
ORIGIN      
        1 gccgccgcct ttcccgtcgg tcccgcccgt cagacgaacc gtgaggccgc cgagtttatc
       61 tacacgcata ttttcaccgc tccactatca gagtttttct ctgggagggt atgttgctat
      121 cgcgggaagg cgagacccta ttgacccaga taccaatatt gcaggaaggc gagcacagcg
      181 accaacgtag cctgtaccac cggctgctgg taaaacggta cgaaggctcc ctgttgaacg
      241 gcatcaattt cgctgatgat ctgccgcagg tgatcactcg acggtcccgt gtcgacggct
      301 ggttcggcac actcctgctg aatcaggaca cgatcgagat cctgctgaag cggtgcgagc
      361 accttccgct tcgcttccac tgcggccaga tataacatgc aggcgctggc gagagccacc
      421 aggctgttga ccgtcaggac aagaatcaag gccggaggaa aatcccaatt gtcgaaataa
      481 tggcttcgtg ccccgataag caggaataac gacaagaagg ggtagaggat ccggcgattg
      541 acgaccctcg tccgctcgat gatgaggcga acttgcctga tggggtcgag accggttgcc
      601 gacggcattt cctggatcca cttggtgcaa aggatcaccg catccaacac cgccaaattc
      661 aagatgatca acgaaaacac actcagagaa gtgagcaccg catccgcatg gcagctgaac
      721 tcccctcggc agggaacata gagccgccat tcgccgtcat tcatgaatct ccacaccgga
      781 agtaccatca tcaaatacag gagcagccac agcgcgattc tcaccgtccg ttgaggccac
      841 gtatgggcac ggcagtagcg ggcccagagc gtacgagcct cgcccgtgcg gtcccgcttc
      901 gatccgtgga acatccaatc aaggttggtc cagaacccct gcaagtttct cggcctcccc
      961 tcttccaccc ctgccgaagc tccctgtggg aagaatttct tggtgaggtc ttctgtgttc
     1021 acggctaggt ccgcgcaggc cttcatcaga aagctgaggg acagcaaggt ggcaaacagc
     1081 cggagcaatt ccgtgggcca gacgctcacg ccgtcggacc aggaaaaagg ctcgtcttcg
     1141 acatagctga agtgccgaag cttacaccac agcagaacca ccccgagaat cagcagcggc
     1201 acccaggccg gcctgagtac tcgcttgaaa ttccgagagc tccgatcccg ttcgtctcgg
     1261 gccgtgagcc aattccaaaa ccttccatag ccccacgccg tcaggaaacc cagaaccgtc
     1321 acagcaatcc aaaccggacc gatccgctcc ggcattctga atgttacagc ccaactctcc
     1381 acatcctgtc gagccggatg gacgctcctt accttatcag ttgaattatc gacactgaga
     1441 tcgactgcgc catgccgtcc gatctcaaaa agacgggggc ggatcacggt gctgtactca
     1501 atctgctgtt gctgaccgcc gttcatcaca tacccggcaa gctgcgcgtt cggaccagca
     1561 ctctggtcca accgataacc gacatgatcg attgcccgca agacagcaag aaaagtcgac
     1621 gtttggaggg agtggcggaa gggcgggatg gcctgctgca atgatggctg caatcgcagt
     1681 ccgaaatggg acacgacgat caggttgcga gtccacttca gctcatcttc gtgggaatac
     1741 cgcgcatcca gatccgttgc aaagaacagt gcgttcggaa actgctcccg caccgcctgg
     1801 aggatcagga gcgcatcata agcatcccgc cccaggattc cgacggcctt gaccttcttg
     1861 ccttgctgtt ccgcttcgcc ccgagtcccg gccttatcat tctcctggtc ctcagtctta
     1921 attcgagcca ccaatcggcg aacatagtcg agttgcgaag gcccttcagg ccgttcatac
     1981 gaagcaatat cgcgaagctt ggccttgcct gcctccccct tctccttgtc tttgtcgtcc
     2041 cccttgagcc tggatggact tccttgatcc accgatgctt ccccatccaa gccgctcagg
     2101 taactgtact gcctgatatt gagggttcgg ggcgagatag ctcctgtctt gaattggtct
     2161 atcccttgtt ccgtcgtcgc acacttcccc ttcaactgac ccgtgagcgc atctgataga
     2221 ggatgtgcct tcgcaacctt ctcatcggtt atataccgac aggcggcagc gctaaaggtg
     2281 agcgggaggg aacgggcata gaaggaatcc cattccccga ttaaggcgat cgaatccgca
     2341 ccgacagtca cgcgccggcg attgagctcc tcaaacaaag actcgaacaa gacctggtcg
     2401 ctgtcgatgc tgtacttgag aagaaggccg gcatcggaca acaattcggt aaacaccttc
     2461 ttgcacgcgc catacgattg gcatcccaca ccattcttct ggcttccgct gttcagtccg
     2521 taggagagta gtccaggcat agccgtggcc catggagaat acagcgcgat gggctcctgc
     2581 acctttggcg gggagtcccc cgctttctgc cttccctttg ctcggtcgct ggacttactc
     2641 tcttcctcaa tctcctggag catgctccga aactctgacg agctgcgggg gcccacaagt
     2701 tttgctggaa tgctcgactc aacgttaggc gggcgaaact ttgagagcag ctgcttgacc
     2761 cgcgcaaaga tagtctgttc ggtctcttcc gccgcaaccc agagaacgag cacatgacgg
     2821 gctttttcct tgctcatctc ttcatcgaat gcccggcagg atgcgatgac gctgcgtttg
     2881 taccattcgt atggtgtggt aacaggtacc ttcggctcga ccttcccaac accaaaactc
     2941 caggtgaagt aagagagtga ttgcgtctgc tccgggacaa aacatccgac ctccagcgcc
     3001 gctcccaccg cataacgatc ccggatgcgc gcctcgctgt cttcaactga agaccctccc
     3061 cccgtcatga ccaccagggc cgtgactatg ccattttgct tggcagtttc attgatcttt
     3121 gcttggagct tgctcagtcc ctcctcttcg ttccggccta ctgctgttac ggagagcgtc
     3181 accttgcccg ctttttgcaa agtgacagac acctgttttc gggacgcttg atccttttgg
     3241 accgctgcaa acgggtcttc ccagagcctc gcgcgagcct tcaattcact agccgcgcct
     3301 tccagcccga ccccgtttcc ggtaggcctc gaacttttca gcggctcctt cacaaacacc
     3361 atcgccgcca cggcgagaac caaggtgaca gtcgtggcca atgaaaaagt ggaatcgtcg
     3421 cccttgaacg ccatgcgcaa tccctgtctt caggcgtgag gttggcggac ataccccgtt
     3481 accactaggg tgattcagca tgtactgtgc cacctgttca atgggtgaat acaagatgaa
     3541 tccgccgctt ttgatgccgg ccctgtcatt gtacgtagga caatgtatcg gcatggatac
     3601 agtgcgtatc caaatggata acgcgcaacg aagggaaacg agaggattgg aagcgggaag
     3661 aggcgctacg acgagcagct gacgctgagg tgtttacccc agttcggggg cgcggcggcg
     3721 taggaattca tgccgggttg ctctgtatag ggctgttgca gcagcgccag taaccgttca
     3781 acctcggaat agtctttctg ctgggccttc tcgatggccc cttgggcgag atagttgcgc
     3841 aggacgtatt tgggattcac ccgatccata cgaatccgtc gctcttcgtc gcgactctgc
     3901 tcgctctgca gccgttcgcg atactgaacg gcccaggtat cgaaccgctc cggattcagg
     3961 aaatgttccc gcagccgttc gttcttcgcg cccgcgtcgg atgaaaacat gctcaattca
     4021 cgccagaaga tcgtgtaatc gacccggctg ccgaccatga gcgatttcag ttcttgcagc
     4081 agggcctcat cttccgcgcg gtcctccacc aaccccaatt tggcacgcat gtggttgtga
     4141 tagtgccgat ccacgctggt ctgataacca tcgagcgcgg ctttcagctc ttccttgggc
     4201 gcaaacggca gcagggtttg cgccaggcaa ctcaggttcc agagtccgat gtagggctgc
     4261 tggttgaagg catagcgccc gttatagtcc gagtggttgc agatgaagcc ggggtcgtag
     4321 tcatccatga aaccgtatgg gccatagtcc agcgtgaggc ccaggatcga catattgtcg
     4381 gtattgagca cgccatgcga ccagccgacc gcttgccagt gcgcgatcag tttcgccgtg
     4441 cgctggacca cctcggcaaa aaaacgggca tatttgtcga ctgcctgcgc aagttcaggg
     4501 aaatgcatct cgatggtgta atcggccagc cgctgcagat gctcgtgctg cttccggtag
     4561 tagaaaattt cgaacgtgcc gaagcgcaca tgcgacggcg ccatccggac gatcgtcgcc
     4621 ccggtttcga tctgttcgcg atagaccttg tcgtcgctgc cgaccaggca cagcgcacgc
     4681 gtcgtcggga tgccaagtcc atgcatcgcc tcgcaacaga gatattcacg gatggccgaa
     4741 cggagcaccg aacgcccatc cccgtcgcgc gagaagggcg tcatgcccgc gcctttgagg
     4801 tgcagatccc agcgttcccc gcggccgttc gtcacttccc ccagaaggat cgcccggcca
     4861 tcgccgagtt gcggcacata gacaccgaac tgatggccgg agtagagcat ggccagcggt
     4921 tccatgccgg gaatcagcag gctcccgccg aacaccccgg cgaactccgg tcgcgccgct
     4981 tccgccgggt cgagatcgag caactccatc gcggcccggt tcgcgctgat gagaaacggc
     5041 gcggccgaga acggcgtcgg gctcaccttg gcgtagaaga cctccggcag acgcgcgtaa
     5101 ctgttatcga acgtgagggt ttcgagacta tggactgtca tcctgcttcg tgatcctgag
     5161 ggttaccgga aattggcttc gatgtccgat tccaacgtga agacaaaaat gcgctccccg
     5221 accacggtgt cgatgtcggt aaacagcgcc gtcactttcg caccgatcag atccatcact
     5281 tgttcgcaga gccgttcccg accttgcgcg atgaggttct gccgaagtcg tttcaccata
     5341 tcgatacctt cggccgtttt ggccagctgg cgttctgcgg gtgtcagcac ccccttcaac
     5401 cgcacgacaa gcatgtcccg cacaatgaag acgcgaacct catccgggcc gcggcccaga
     5461 aattcctgct cgaacttgat gatggccgtc cggacagcgg cttccttctc tcccttgctc
     5521 gccacaggca atagatcctt ctgaatggtt gatcgagaca acgcgccgcc gattatagtc
     5581 accgccatgt gcagactcca acccccttcc accgctgtcg gcaaacgatc cggcacgacg
     5641 ccggcgacca ggcccatggg ccttgccagc aagccctgcg gcgccgactt tttacttgca
     5701 attacaaagg cgcgcggcta catctaccgt agttttccgg tggtgccgcc gagaggccgt
     5761 gaatcgcgcg tggcccggac ccaccacatg gatgtgatga gacaggcagt agggtcttgc
     5821 cggtgtttcc ctccagggaa aagccgggga cagtgcaggc cagtcgttgg ttccttgttt
     5881 caaggaccgt cggctggcct ttttcttttc atgagtgcca tggggtcggc atgtcgtttc
     5941 tagcgctcgt cccgctgttg ccgttgttga cggccttcat cgtgatgacc ggcgaccgtg
     6001 cggagcagga ccagaatgcc agagccgggc tgctccccgt cgcggcggca tttctcgggt
     6061 ccctcatcgc cctggttgcg gtcacctccg gagatccggt caccatccag ttctacgata
     6121 cggcctccgt cgccaatctt gccttcccca tcgggttcta tatcgaccgg ctcagcgcgg
     6181 tcatgatggt tctcatcacc ggcgtgacca tgctcatcta ccgctattcc atgggttaca
     6241 tgtatcagga tcgcggatat cgccggtatc tcgggatgct cggcatcacg accgcggtgc
     6301 tcctctgcat ggtgtccagc gccaacctgg tcatgttatt tgtcttctgg cagatcctct
     6361 cgtggctgct gtttctgctt gcacacaacc acgggcatgc cgccacactg tccggcgcga
     6421 cgaacacgtt tacgatgttg cggctcagcg atgcggcgtt tctggccggt atcgtcctgg
     6481 cctattcgct gtacggcaca ttcgagttcc agacattgtt tgcccgcgcc gcggacacgc
     6541 ccgtcaccct gtccctttgg ccggacctcg gctgggagat gaacggcgcg acggcggtga
     6601 ccctactgat tttcgtcggc gccatgggca agtcggcaca atttccgatc cacacctggc
     6661 tgccgcgctc cctctacgcg ccgacgccca tccacgcact gctgcacgcg ggcatcatca
     6721 acgccggggg attcctgctg aatcgtctgg ccccgctcta cggtctgagc ccgaccaccc
     6781 tgcatgtggt gttcgtcatc ggcatgctca cggcgattct gggagccacc atgatgttga
     6841 cgcagaacga catcaagaag acgctcggct tttcgacgat cggccagatg ggctatatga
     6901 tcatggaatg cgggctgggc gcgttttccc tcgccgtgtt ccatctgatc gcccacggtc
     6961 tgttcaaggg cacggtgttc ctgaattgcg ggaatgtgat ccacaaggct cgccaggaac
     7021 cctcgtttcc gccaatcgac cgcgaggcgg aggaaagcga attctccaat ctcacctggt
     7081 ccaccggatt cctgacgacg ctgctcttgc cgctcgtgat cctgctcgtc acgcacggtg
     7141 tcttgcgcat tccgctgatc gattcacaag gcacggtgat tttcctgttc ttcatctggg
     7201 ttacctcctc gcaggcgatt ctctccctga cacgtattcg cgccgtggcc tcctggaagg
     7261 tgtccgccgc catgctggtc acgctcgtca tcgtcgtctt cacatacctc tttgcggtgg
     7321 agagctttac gcattttctg tatccgaatc cggaggtggt ggcgtcgtat ttcaaggccg
     7381 cggcgctgcc gggccggctc ttcgacagtc tggtggtcgg cacgacagtg ctgacgatcc
     7441 tcagctgggt ctatctctat gcgcatgcac atgggcgcac ggtcaaaatt cccggctgga
     7501 tcgacgtctt cctcgttcgc ctctatgtcc tgttcatgaa ccgcctctac ctggatcagc
     7561 tgtatctcaa gttcggccgg atcgtgacgc tcgtcgccca tcacctggaa aagcggctgt
     7621 cgtgaccatg caacgcagcc acacgaacaa cggcgcggcc caggctgtcg gaggcctgcc
     7681 atgatcaaga cctggatgac accgtggctg ctcatggccg ttcccttcct cggcgcgacg
     7741 ctcagcctgc tcctccgcac gtcgctccag cgaatgaaga cctcagccct cctgacgacg
     7801 gtcgctagta tgctcgtcgt catcgggacg ccctgggcgc tgggcgacac gctggccggt
     7861 gtgccttttc tctgcctgct cccgctgacg gcgtttcttt cgctcctcgg ccaaccgtta
     7921 caccgcgaca atacttcggc ctggacgacg acgctggtgc tgttgggact gggactgggc
     7981 atcctcactg ctcaagcgcc ggcgcacgac gtcatgctct tgatactgcc cggcttgctc
     8041 tgcatcctgc tgtatcggta tcaatccggc gccacgccgg agacctggcg gggactgtcg
     8101 gcctacggac tgagcatggt gtcggctgcg ctggccctgg tgctcccgac accggcctcg
     8161 acggtggctc tgttggtcac ctgtgccaca ctgctgccgc tcctgcccgt tcacagcgga
     8221 ttcattgcga ccttgaccgg attaccgggc aatctccccg cgttcctcgt cttcctgctg
     8281 ccgaccgtcg gattccatac cctgctgatg ctgctcccga acctgaccgg cacgaccctc
     8341 cacaccgtgg cgattctggc attggcgggc gccgtgtacg gttcgctccg ggcgctgatt
     8401 caggcgcgcc cgctgccgcg gctttcctac gccgccctcg cgtttttcgg cctgctctgg
     8461 tggtatgtcg ccgacaccgg caccgccccc atgcaagcca cggtctatct cagcgccgcg
     8521 gggctcgcgg ccagcggact gctgctggcc tggtatgcca tccgggcccg gtatggcgat
     8581 atcgatctcc gcgcactcgg cgggctcgcc tatcccatgc cgcgcttcag taccctcttg
     8641 gcgctcctgg cgctcgccgc gctgggcatg ccgcctttcg gcgtgttttc cgggttcctg
     8701 ggcatgttgc tcctgcccac gtttacaccc tccgggccct ttgccgtcgt catgatcgtc
     8761 tggctcacag cctcctggta ttacaccgat ttcctgcaac aactcatctt cggccggcaa
     8821 cgcatggata tgcgatacga cgacttgcgc cagacggagt tcgcctcgtt ggtgttgctg
     8881 ctgttgttgc tgctggccct cggtacggcc ccctcgcgct ttttcgactc gtacataccg
     8941 acacccccgg ccactgtcgc catgcaggag gggacatgga tccggtgatc cgcgccgacg
     9001 acccggcaga cctcgaagcc cgccgcatgg aactgcgagg cacgatccgt ctcgccagcg
     9061 aagtcatcgc gcagtattgg ccgatgcgaa ccttcgtcca tcacaatccg ctccacagcc
     9121 tggagtatct cccgttcacc gaaacggtcc gacgtggcca ccaattcctg ggcggcaacg
     9181 ggtatctccc cggtgacgtg tatcggcgct atctgaaatc aggcaggatt ctggtccgcc
     9241 atatcgacga ggccctggcg cccctcgcgc tcgagcagga agtcgatctc ggcccctgcc
     9301 gcattgccca tcgagaagtg ttgcgagcct gcctgaccca tggtctctct accgcgaccg
     9361 acgaaccact cgatcgattg atggaacgtg agccgaacga ggagcaggtc gaggcactgg
     9421 ccgaacgact gggctcgttc tccacaccaa cggtggcgga acggatggcc gccacggtcc
     9481 gtgcggattc ggatgctctc ggacatcatc tcaccctctc caactggtgc gaccagacac
     9541 tgggcacccg gatcgtcgaa ctgatcaaca gcgaactaat caaatggtgc gaagcgtttc
     9601 tggacgaagg ccacgccacc tggccgatgc cgggtcggga gcagggcctc tacgccgcct
     9661 ggaagcaagt ggccgggaaa gaatggacaa cgtgcggcat tgcggacagc cgccggaaaa
     9721 ttgcggcgct gccggaacat cccgaagacg ccgtattgga ctgcctggac gcgctggcaa
     9781 tccccgtgac attccggcag gactacctgt cgctccaact tgcagccctc ccgggttggg
     9841 ccggcttcat caaatggcgc gcggaagaaa ccggctatgc ctggcagcag gcctaccccg
     9901 tcgggctggt gaagtttctc gccgtgcgct tgtggtatgt gcgcgaactg gtccagaaag
     9961 tctgccggga agaattgggc atcgacggga actatcccgc ggtgatggcc tacatggcgc
    10021 agcagtcgca tgcgtacttc atgcacaagg aatgggccgc cggacgactt cccgcctcca
    10081 tggccgaccg tgtcgcccgg ctgcatgccg acacgagaca ccgtcagcag ggcacgccgg
    10141 ccctcgcccc tgaatgggag ccactcaccc atcacgatca gatcgaattc ggaccgcgcc
    10201 gcgaacgggc tgcgcagcgg ctcatggcgc gacgcctgct caccctggcc caagcgctcg
    10261 agatcgttcc ggccctgctg atggacggtc cgttgacggc ccttcgcacc ttgctgaatt
    10321 ggatcgacgc ctttccggaa tccgcgcacg gtccggtgtg gctcaaggcc ttcgaggccg
    10381 ggtatcagga tcacctcttc ggcatgttgc tgcgcgcgcc ggtcaaaccc caaccggtct
    10441 ccgccgagca ccatccgccg gtgcggcccc attcccaatc ggtcttttgc atcgatgtgc
    10501 gctccgagcc gttccgccgc cacctggaat ccaccggcgc gaacgacacc tatgggtttg
    10561 ccggtttctt cgcggtcttc atccggtatc gggcctgggg caaagaacac gagaccgagc
    10621 agttcccggt catcatgcgc gcgaaaaacg aggtgcggga gatcccccgc agctatctgg
    10681 atcactacgt gtccaaacac cagtcgcgcg cgaaactggt ccacgcggga catacgctgc
    10741 tgcacgacct caaggagaac gtcgtcaccc cctatgtcat ggtggagtcg ctgggctggt
    10801 tctacgcgct gcccatgatg ggcaagacga tgtggcccgc cctctacaaa cgattgacca
    10861 attgggtgcg gcggttattt gtccccccca tcgccacgat tctcacggtg gacaaactgg
    10921 cgccggccga aaccgaggaa atgatggtct ccgaacagcg ggccttgatc tggaaagccc
    10981 tgcgcgatcg actcggcctg catggatcgc gggtcgaggc cgaattcgtc gaggccctgc
    11041 ggcggcgtgc gctggacgac gatgcccccg tggaaccgtt cctcagcgac gcggccaaat
    11101 cggtcgatct ctccgccgac caactcacca ccttcctcga agaactccgg cggcactacc
    11161 ggatcaatcg ccgcgcggcc tcccgccaga aagaacggat cacgcggacc ggcttcacgc
    11221 tcgaagaaca ggttctgacc gtggaaaccg ccctgcgcat gatgggcctg gtgagaaatt
    11281 tcgcccgcct cgtgctcttc tgcgcccatg gcagcaccac cgagaacaac ccgttcgagt
    11341 cggcgctgga ctgcggcgcc tgcggcggaa acgaaggcaa accgaacgcg cgcgtgctgg
    11401 cggcgatggc caatcgcccg ccggtgcggg aacggctggc gaaacgcggc atcgaaatcc
    11461 cgtcagacac ccacttcctc gccgggcagg tcgataccac gacggacgag gtgcacctgt
    11521 tcgatctcga agatgcgccg ccgacccacc gcaaggacgt ggcccgactg tacgacgatt
    11581 tacgcgaagc cgcgcagctg accagccagg agcgctgcag ccggttcccc gatgtgagga
    11641 ctgtcctgcc cctcaaccag gcctcggcgc atgtagccgg acggagcgcg gactggagcc
    11701 aggtgcggcc ggaatggggc ctgtccggca acacaacttt catcatcggc cgccgggagc
    11761 tgaccaaggg attgaacctg gccgggcgcg tcttccttca ctcgtacgac tatcgggaag
    11821 atccgacgga ccgttggctg gaagtgctgc tgactgcgcc gcaggtggtg gcgcagtgga
    11881 tcaacatgga gcattatttc tcggccgtgg acaacgaggt ctatggaagc ggcagcaaga
    11941 tctatcacaa cgtcgtcggc cgcatcggca tcatgtccgg cccctggagc gatctccggc
    12001 tcgggctggc ccggcaaacc gtcatgaacg acgacctgcc ctatcatgaa cccatgcgcc
    12061 tcctgacgct ggtggaaacc tcgcgcccgc ggatcgagaa actcattgcg cggcacgaag
    12121 tgctgcaaca tttctatcac aacgagtggg tgcacctggc tgcgctggac cctgaggacg
    12181 gcatctggta ccgctacatg ccctcgggtg tctggcgccg cgtgaggaat cccagtgaca
    12241 catagattgc aaccggcatc acctcggaag agacctcttt tcaaaggagt ggagacatgg
    12301 ccgcactcac gttgcatccc atgaaagaaa ttcgcgtcat cgtgtcaggt gagcaccggc
    12361 cctttgtcac cgagttattg gacaaagtgg aggcgaccgg ctacaccatc atcggcaata
    12421 tctcggggaa gggccatcat ggcctgcgtg aagcccattt catgttcagc gagcaggaga
    12481 gcctggtcat gattatggcg gtggtgccgg aggaaaaggt ggaaccgctg ctggccgggc
    12541 tccggccgct gttcgagcga cattcgggtg tcatgttcgt ctccgatgtc gccgtcagcc
    12601 ggcgcgaata tttcggcaag aagagcgcca agtcctgacc ggcagaccgg gaacccgcgg
    12661 ctggtgagcc cgcgggccgg gcagccgacc ttgtcttctc accggtgatt gtctacaatg
    12721 tgcccccgtt ggcctgtaag aggaaggacg gcacgtgaag ggattacggt ttgaacggct
    12781 cgggcgagat cgccactaca acatcatctt ccacatcggc agcagctatg tgccggtgag
    12841 cgacgagacc atcgaagagc tgaaggctca gaccctgctg tcctccgagc gtttcctcga
    12901 cctcctcctc gacaaaatcg ggtacaccag ttacctcaaa gaccagatcc gcacggagct
    12961 tcaagcctcc ggagatccca tgacccagat gaccgtcctc ca
//
LOCUS       DAOQRM010000067        12916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3936_length_12916_cov_15.791540, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000067 DAOQRM010000000
VERSION     DAOQRM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12916
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3936_length_12916_cov_15.791540"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1536
                     /gene="purD"
                     /locus_tag="PKK81_07050"
     CDS             259..1536
                     /gene="purD"
                     /locus_tag="PKK81_07050"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNV32668.1"
                     /translation="MKILVVGGGGREHAMVWKIAQSPRKPQIFCAPGNAGIEGLATCV
                     PITADDIEGLKAFALKEQIDLTVVGPEAPLALGIADEFRKARLKIFGPTKAAARLESS
                     KSFSKDIMAANRIPTAAARSFEHMDQALAYLDEQPVPIVVKADGLAQGKGVVVATTRE
                     EAKQAVRDAMEKSLFGQAGHRVLIEEFLDGEELTLMAFTDGKTVVPMVPAQDHKRVGD
                     GDSGPNTGGMGAYAPAPIATAALREQVTRQVLQPTVDALARLGCPFQGVLYAGLMVVK
                     GTPYVLEFNARMGDPETEVVLPLLKTDLVEVMEAVVEHRLDELTVEWHPDTAVCVVMT
                     SPGYPGSYPTGLPIQGLPTPFGESRVAVFHAGTKRESGRVVTAGGRVLAVTAWGPSLL
                     DARAQVYQAVPSILFEGRHYRTDIAHRALPHKI"
     gene            1563..2210
                     /locus_tag="PKK81_07055"
     CDS             1563..2210
                     /locus_tag="PKK81_07055"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNV32669.1"
                     /translation="MALILPFTDTTCDRVLPDARQVLDAHGVLALPTETYYGLAVRPT
                     DETALRRLIELKGRPSDKPILVLIGSLDQLPQLVQAIPPAAALLMEQFWPGPLTIVFP
                     AAPDLPALLTAGTGTIGVRQAPLLQLQHLLRHTGPLTGTSANRSSEPPLDNAVAVQRA
                     LGAGVDLILDGGHTPGGLPSTMVDARDRPHLLRAGALSTDLIRAALARQGYELSS"
     gene            2259..2702
                     /locus_tag="PKK81_07060"
     CDS             2259..2702
                     /locus_tag="PKK81_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32670.1"
                     /translation="MLIRSRRAVMGLSTLGVGAMLLLSGCDFWPPALQAQIEQLHSEA
                     QQAAADKALLQNQLNAANKAKEELQIRVEDLTRVNKEKSAMIANLEHTIAAARERAVK
                     ATKSSAVKPVAKSAVKKSVKPTHKPAAKSSVRSHSTKKTKPAPVR"
     gene            3026..3259
                     /locus_tag="PKK81_07065"
     CDS             3026..3259
                     /locus_tag="PKK81_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32671.1"
                     /translation="MLEDDEELEPGELVPDVDVLVLVDVVELGAAAGAGAGVVVELVP
                     PPSEEAGAFVLSVLDAAGFSEVSLPEPGFILSE"
     gene            complement(3561..4754)
                     /locus_tag="PKK81_07070"
     CDS             complement(3561..4754)
                     /locus_tag="PKK81_07070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNV32672.1"
                     /translation="MKLAARVGRIVPSPTLSITATAKSMAAQGIDVIDFASGEPDFDT
                     PEPVKAAAEAAIRAGFTKYTPSSGIDELRVAIADKLKEEQGLQYDKSQILVSCGAKHS
                     LYNLAEALLEAGDELIIPVPFWVSYQDQTLLNDATPVLLQTREEDGYTISQEALEAVI
                     TPRTKAIIVNSPCNPTGATYDRTTLEGIAAAALRHDLVIISDEIYEKVLYDGTQHISI
                     ATLSPEVAARTVVINGVSKAYAMTGWRIGYAAGPKPLLTAMANIQSQSTSNPCSISQK
                     AAVAALRLGNPFTKTMVTEFDRRRRVMVDRLNKMPGVTCRMPTGAFYAFPNVSGLLGK
                     RWKDQPIGSAANLATYLLNEAQVAVVPGEPFGSDIHIRLSYATAMEAIERGLTRIDAA
                     LRRLS"
     gene            complement(4751..5239)
                     /gene="coaD"
                     /locus_tag="PKK81_07075"
     CDS             complement(4751..5239)
                     /gene="coaD"
                     /locus_tag="PKK81_07075"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNV32673.1"
                     /translation="MKTAVYPGTFDPITHGHSDIIRRGFRMFEKVIVAIAPNPGKHPF
                     FSVKERLEMVGLVTKDLPNLEVTTFEGLLVDFVRECGAHAILRGLRAISDFEHEFQMA
                     LINRKLAETVETVFLMPSEEYSYLSSTIIKDVASHGGSLQDFVHPEVARRLQERIRSF
                     KG"
     gene            complement(5236..5808)
                     /gene="rsmD"
                     /locus_tag="PKK81_07080"
     CDS             complement(5236..5808)
                     /gene="rsmD"
                     /locus_tag="PKK81_07080"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNV32674.1"
                     /translation="MRVIAGLHRGRRLLGPRGQAIRPTSDRVKEALFSILGERTTGAR
                     VLDLYAGTGSIGIEALSRGAAHATFVEADRQALRLVNSNLEQCGLQQSANVCACQVSQ
                     FFRRATQWSGPYDIVFCDPPYQLTPELIAMAQEWNAGWLADDAVVIIEHGKKAEIPQT
                     LGLLSQVKRYDYGDTALTRFHVTAKEAQPR"
     gene            6478..7194
                     /gene="radC"
                     /locus_tag="PKK81_07085"
     CDS             6478..7194
                     /gene="radC"
                     /locus_tag="PKK81_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNV32675.1"
                     /translation="MTPKKTGRGIGKWPETERPRERLLREGAESLSDAQLLAILLRVG
                     RQEASAVKVGMEVLDRVGGIFGLLHCSAEELCAIPGVGPAKAAQLKAAVEVGKRAVSA
                     PLTTGTRISSSADLFKHYHARLRDLRHEIFAVVLLDAKNQVIRDVTISEGSLTLSIVH
                     PREVFIPAMRASAAGVIFLHNHPSGDPTPSQEDRVLTARLVSAGSLLGIQVLDHLIVG
                     DGRYVSFADQGWLNGEGARV"
     gene            7328..9433
                     /gene="fusA"
                     /locus_tag="PKK81_07090"
     CDS             7328..9433
                     /gene="fusA"
                     /locus_tag="PKK81_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV32676.1"
                     /translation="MNVPLTESIRNVAVVSHAGSGKTSLVEALAYCAGSLSAPGSIFA
                     GTTVSDFEPEEIHRRTSLNTTVLRCSYQDRVLTLLDTPGSPSFIGETRSALRAADGVV
                     LVVSAASGVRSALQRIWSMVRDSGLPCVVFVNDLDKDGTVFDNTVEDLTKELEMTAVP
                     LILPMGSGAQLGDVVDVLQNHVVTSQPDRPKSQQGPVPPEWRDRVDQARRRVMELVAE
                     GDEALLERYLTDGALTEEALLEGLQAGVQRGSLVPIVGGSALRHIGVHSLLHTLVDLL
                     PSPVSRARVAPLQGTGSMEDGGPINRHPLPTDPFSAVVFKTLIDPFIGRLSYVRVYSG
                     TLEADTAMYNSTRQVRERGGHLFSIFGKKYTPITRATAGEIVAIGKLKDTVTGDTLCD
                     EHAPIRYPGIPFPRPVVSFAIEPKSKTDIEKVSLGLHKVIEEDPSLEFVRHIETKEMV
                     LSGMGQLHIDVALEKLHRKYGADVIVHAPKIPYRETIRSVAQAQGKYKKQTGGHGQYG
                     DCWLEVGPLPRGQGFEFENKIVGGAIPRNFVPAVEKGVVEALHEGPLAGYPAVDVRVT
                     VYDGSYHVVDSSELAFKIAGSMGVKKALEAAHPILLEPLMTVEVEVPAECVGTVLGDL
                     NARRGRIVMVEANGHIEIVKALVPQAEMLNYAASLNSMTGGQGSYAMEYAQYEEVPRE
                     LAGRIIEEHKMERHGAVAH"
     gene            complement(9477..10883)
                     /locus_tag="PKK81_07095"
     CDS             complement(9477..10883)
                     /locus_tag="PKK81_07095"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNV32677.1"
                     /translation="MKTQTSFVTALILALGFSGWLTGTSAITLAATDIASPPRKESPG
                     LRMLEELQTVITDLAEEAKPSVVSIFPIQTLGKSRDGSGERVPNSTGSGSGVIVDPNG
                     HIITNNHVVGDATEVEVRLSDKTKLFAQVVGKDPDTDLAVLKVTTDHPLPAARFGDST
                     GVKVGQWVLAVGNPFGLDRTVTLGVVSGIGRENINLSRYENFIQTDASINPGNSGGPL
                     FNLRGDVIGINTAIINFAQGIGFAIPSNMAKQVMNQLISKGKVVRAWLGVGLQPLTPD
                     LANKFGVDENEGVLVNEVFERDPAALAGIKPGDVITKVDGALVDTPNKLSRLVAALDP
                     GSTTHVEVVRDGKRLTLNVALSERRDAVVTASLPQSRTDLKLGIDVQDLTAGLAEKFR
                     LNESRGVLISKVEAGSLAQAEGLREGDLVKEVNRVDTGTVGEFTVAVSKVKRGDTILL
                     RVLREGRAFYVVLKPADR"
     gene            complement(10971..11609)
                     /locus_tag="PKK81_07100"
     CDS             complement(10971..11609)
                     /locus_tag="PKK81_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNV32678.1"
                     /translation="MLSFELLARVRKELFITGSALYETIVAIAERVNRKVHVLRLHGQ
                     ATTILNQIHAIHRQVGRRIAELGTKPSGAIPAVAPGTLPSLENTIRTASDRIKGQQTA
                     LQQIERHIRDLKIELAHEDLLTMQRELGLRDAAIERVLVARGAPVIGHPIGEFDLPST
                     THIVAVFRGPFLLPLSDSMTLRPDDIVILVGLRHDLAHWLPHFQRPAASKSA"
     gene            complement(11639..12241)
                     /gene="plsY"
                     /locus_tag="PKK81_07105"
     CDS             complement(11639..12241)
                     /gene="plsY"
                     /locus_tag="PKK81_07105"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNV32679.1"
                     /translation="MDSPWLIGFLILSSYLLGSIPFGVVVSRLLGAVDPRSAGSRNVG
                     FTNVLRVSGKKAGILTLLGDIGKGWLAGWVGTMLLHQESAILWVALASVIGHLHSAFL
                     GFKGGKGVATALGAALGVAPWIGLTLMGLWVGAVFLWKYSSGGALFAFAMFPIVALLF
                     HRSWTFIGFACVVSLLIWVRHKDNLVRLWSGTESRIGQAS"
     gene            complement(12290..>12916)
                     /gene="pgsA"
                     /locus_tag="PKK81_07110"
     CDS             complement(12290..>12916)
                     /gene="pgsA"
                     /locus_tag="PKK81_07110"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNV32680.1"
                     /translation="VEGFMAENWKQAGLVLMRSAGQESNINLPNVLTLVRILLIPVFV
                     MLLIDPTPDRALAAAIVFVVAAVTDLLDGYVARKTGQITKLGRLLDPIADKLLVLSAL
                     ILLVQVDRVSALVAILIIAREVAVTGLRAIAASEGLIMSAEVTGKYKMALQVIAIVLL
                     VLEGTVVEVIGNLHLAGIVTLYLSLILGYVSGAQYVWSFWRQVGAKGL"
BASE COUNT         2531 a         3679 c         4052 g         2654 t
ORIGIN      
        1 gcgccacttc cgccattgag gggcgttgaa acgggcctcc aacttcgttc tcggcgagaa
       61 aaaatcctca acgtagccct gaggctacgc ctccggtttt ttcttgcctg cggccttgtt
      121 ggccatcccg tttgaacgcc ctcagggacc tgcgtggttc aggctgatcc ggacgggcag
      181 cgatgcgtct cagcagttcc atcgagtctg tcagtcgcag aacgctgatc tcttttgatg
      241 taatgtaaag gttgagctgt gaagattctt gtcgtcggcg gtggcgggcg cgaacatgcc
      301 atggtgtgga agatcgccca aagtccacgg aagccgcaga ttttttgtgc gccggggaat
      361 gccggcatcg aaggtctcgc cacctgtgtc ccgatcacgg ccgatgatat cgagggtctc
      421 aaggcgtttg cgctcaagga gcagatcgat ctcactgtag tcggcccgga agcgccgttg
      481 gcgttgggca tcgcggatga gtttcgcaag gcgcgactaa aaatcttcgg accgaccaag
      541 gccgccgctc gcctggaatc cagcaagagc ttctccaaag acatcatggc cgccaatcga
      601 atcccgaccg cggccgcgcg cagcttcgaa cacatggacc aggcgctggc ctatctggac
      661 gagcagccgg ttccgattgt ggtcaaggcc gacggtctcg cgcagggcaa aggggtggtc
      721 gtggctacga cacgcgagga agccaagcag gccgtccgcg atgccatgga aaaatccctg
      781 ttcggtcagg ccggacatcg cgtgctgatc gaagagtttc tggacggcga agagctgacg
      841 ctcatggcct tcaccgacgg gaagaccgtg gtccctatgg tgccggctca ggaccacaaa
      901 cgagtgggag acggcgacag cggacccaat accggcggca tgggcgccta tgctccggcg
      961 ccgatcgcca cggctgcgct gcgtgaacag gtgacgcgtc aggtgctgca gccgacggtg
     1021 gatgcgctgg cccgtctggg ttgccccttt caaggtgtgc tctacgcggg cctcatggtc
     1081 gtgaagggga ccccctatgt gctggagttc aacgcgcgta tgggagatcc tgaaactgaa
     1141 gtcgtgctgc cgctcttgaa aaccgatctg gtcgaggtga tggaggcggt ggtcgagcac
     1201 cgtctggatg aattgacggt ggaatggcat ccggatacgg ccgtgtgcgt cgtgatgacg
     1261 tcgccgggtt atcccggttc ctatccgacc ggccttccca ttcaggggct gcctactcca
     1321 ttcggtgaat cgcgcgtcgc ggtgttccac gccggtacga aacgggagtc ggggcgcgtg
     1381 gtgactgcgg gaggacgagt tctggcggtc accgcctggg ggccgtcttt gctcgatgcg
     1441 cgggcgcagg tgtatcaagc ggtgccttcg attttgtttg aagggcgcca ttatcgaacc
     1501 gatattgcgc atcgggcctt gccgcacaag atctgattcg agcagttttc ggccattatt
     1561 ccatggctct gatccttccc tttaccgaca caacctgcga tcgcgttctg cctgatgccc
     1621 gacaggtgtt ggatgcgcat ggcgttctcg cgctgcccac cgaaacgtac tacggtttgg
     1681 cggtccgtcc gacggatgag acggcgctcc ggcgtctcat cgagttaaaa gggcggccat
     1741 ccgataagcc gattctggtg cttatcggaa gcctggacca attgcctcag ttggtccagg
     1801 cgattcctcc cgccgcagcc ctcctgatgg aacagttctg gcccgggcct ctgaccatcg
     1861 tgtttcctgc ggcgcctgac ttgccggcgc tgttgacggc ggggaccgga acgatcgggg
     1921 tgcggcaggc tcccttgctt cagctccagc atttgttgcg gcacaccggc cccctgaccg
     1981 ggaccagtgc caatcgctcc tcggaaccac cgctcgacaa cgccgtggca gtgcagcgcg
     2041 cgttgggggc aggggtggat ctcattttgg atggcgggca cacgccggga ggtctccctt
     2101 ctaccatggt ggatgcgcgc gaccgcccgc acctgcttcg ggccggggcc ctgtccacgg
     2161 acctgatccg tgcggcgctt gcgcggcagg gttacgaact ttcatcatga tttgattctc
     2221 ggttgtcggt ataattcacc ctcatgggag gatcacttat gctcattcgt tcacgtcgcg
     2281 cggtgatggg actgtctacg ctcggggtcg gtgcgatgct tctgctgtcg gggtgcgatt
     2341 tctggccgcc ggctctccag gctcagatcg aacagttgca ctcggaagcc caacaggcgg
     2401 ccgcagataa ggcgctgctg cagaatcagc tcaacgccgc gaacaaggcg aaagaggaac
     2461 tgcagattcg ggtggaagat ttgacgcggg tgaataaaga aaagtccgcg atgatcgcga
     2521 acctggaaca cacgatcgcc gccgcaagag aacgcgcggt gaaagccacg aagagcagtg
     2581 cggtcaagcc ggtcgcaaaa tcagccgtga agaagtccgt caagccgacg cacaagcccg
     2641 ccgcgaagtc ctcggtcagg tcccacagca ccaagaaaac aaaacctgcc ccggtgaggt
     2701 aaacacccgt cgcttctgtt gccggtggtg tccttccctt ctgcttccgg gactggtaca
     2761 ccgttgaaaa attccgtcaa cggcggtccc gcctggttca gggcctccac gtactgcagg
     2821 gggacgcgtc gggctagaac gctgtcagcg taccgtctcg ccggcgtcca gcgacctggg
     2881 gctcctctgc cgtcgcgccg caggcttcgc tgcggctcca ctgggcagcg agtttgaacg
     2941 gcctgaggag gatgtgcagg gaatgcttga ctgccgccgg tgctgaagcg cgcagggggg
     3001 caaccatctg tcaagaacct gctaggtgct tgaggacgat gaggagcttg agcctggtga
     3061 gctcgtgccg gatgtggatg tacttgtact cgtcgatgtc gtcgagctcg gagctgccgc
     3121 aggggcagga gcaggcgttg tcgtagaact cgtgcctccg ccttcagagg aagccggtgc
     3181 ctttgtctta tccgtgctgg acgccgccgg tttctcggag gtatcgcttc ccgagccggg
     3241 tttcatcttg tccgaatagt cggtgatata ccacccgctg cctttgaaca tgatggcagg
     3301 ggaagagatc agcttcgtca cttcttttcc gcaacgcaca cattccttaa tgggctcgtc
     3361 cttgatgctt tgcttcacct cgaagcggtg ggcacagctc gtacattgat attcatatat
     3421 gggcacgggt cattcctcct ctggtcagct agcgtcgcgc gctgtcctga tgctcgattt
     3481 gccgggatcc gcggcgccat cttgtagtag ctttgattga gccaaaatca agtgctacga
     3541 ggtattcagg tcctctgagc ttaggacaac cggcgaagcg ccgcatcgat acgggtcagt
     3601 cctcgctcga tcgcttccat ggcggtcgca taggacagcc gaatgtgaat gtcgcttcca
     3661 aacggttcgc cgggcaccac cgccacctgg gcctcgttga gtagataggt cgccaggttc
     3721 gccgcggagc cgatgggctg atccttccag cgcttgccca acaggccgct tacattcggg
     3781 aacgcataaa acgcccccgt gggcatgcgg caggtcacgc cgggcatctt gttgaggcgg
     3841 tcgaccatga cgcgtcggcg ccggtcaaat tccgtcacca tggttttggt aaagggattc
     3901 ccgaggcgaa gcgcggccac cgcagctttc tgtgaaatcg aacaggggtt cgaggtgctc
     3961 tggctttgaa tattggccat ggccgtcagc agcggctttg gtcccgcagc gtacccgatg
     4021 cgccagccgg tcatggcgta ggctttcgag actccgttga tgacgacggt tcgagccgcc
     4081 acttccgggc ttaaggtcgc gatgctgatg tgctgggtgc catcgtagag cactttctcg
     4141 tagatctcgt ccgagatgat caccaggtcg tggcgcaggg ctgcggccgc aataccttcg
     4201 agggttgtcc ggtcgtaggt ggcgccggtc gggttacagg gactgttgac gatgatggct
     4261 ttggttcgtg gcgtgatgac ggcttccagc gcttcctggc tgatcgtata cccgtcttct
     4321 tcccgcgtct ggagcaggac gggcgtggcg tcgttgagca gggtctggtc ctgataggag
     4381 acccagaagg gaaccgggat aatcagttca tcgcccgctt caagcagggc ctccgccagg
     4441 ttgtagagcg aatgtttggc gccgcaggag acgaggatct gggacttgtc gtactggagg
     4501 ccttgctcct ccttcaattt atccgcaatc gccacgcgca attcgtcgat ccccgacgac
     4561 ggcgtatact tcgtgaagcc cgcgcgaatg gctgcttctg ccgcggcctt caccggttcg
     4621 ggggtatcga agtccggttc tccggacgcg aaatcgatga catcgatccc ttgtgccgcc
     4681 atcgacttgg cggtggcggt aatactgagg gtcggggagg ggacaattcg tccgacacgg
     4741 gctgcaagtt tcatcctttg aaactccgaa ttcgttcttg gagcctccga gccacttcgg
     4801 gatgcacaaa gtcctggagc gatccgccgt ggctggcgac atccttaatg atggtggaag
     4861 acaaatagga atattcctca ctgggcatca ggaagaccgt ttcgacggtt tccgccagct
     4921 tgcgattgat gagcgccatc tgaaactcat gctcgaaatc ggaaattgcc cgtaggccgc
     4981 gcagaatagc atgggcaccg cattcccgca caaaatccac cagcagccct tcgaaggtgg
     5041 tgacttccag gttcggcaga tctttggtca ccagtcccac catctccagt cgttccttca
     5101 cgctgaagaa cgggtgtttg cccgggttgg gcgcgatcgc cacaatcacc ttctcgaaca
     5161 tccgaaatcc gcgccggatg atatcgctgt gtccatgcgt gatcgggtcg aatgtgccgg
     5221 gatagacggc ggtcttcatc ggggttgggc ttctttcgct gtgacgtgaa atcgtgtgag
     5281 cgcggtgtcg ccgtagtcgt accgtttgac ctgcgagagc aggcccaggg tctgcgggat
     5341 ctccgctttc tttccgtgct cgatgatcac cacggcatcg tcggccagcc atccggcatt
     5401 ccattcctgc gccattgcga tgagctccgg ggtcaattgg tagggcggat cgcagaacac
     5461 aatgtcgtag gggcccgacc actgggttgc ccgacggaaa aactggctga cctggcaggc
     5521 gcagacattg gcggattgtt gcaggccgca ttgctcaagg ttcgagttga ctaggcgcag
     5581 ggcctgacga tcagcttcca caaaggtcgc atgtgccgcg ccgcggctca gcgcttcaat
     5641 tccgattgag ccggtaccgg catagaggtc gagcacacga gctcccgtgg tccgttcgcc
     5701 aaggatcgaa aagagggcct ctttcactcg gtcggacgtc ggtcgaatcg cctgtcctct
     5761 gggaccaagc aaccgtcggc cccgatgaag ccctgcgatg acgcgcatga tcgtattcgg
     5821 gtgagagcca ttcgtctctg gctaagaagt gagagactct aacacagcgt ttttgaaggg
     5881 gtcaaggtga cgaggtgtga tcacggagat ggaagcgtcg aaaaggtctt ctccccgccg
     5941 cggggcgggg agcgctgcag acgtcgacga ggagtgggcg tgctttgacg cgtaggaagc
     6001 ggctcgctat aatcgggcga caccaagtct cggttcacgc gggctgagga ggtggccgaa
     6061 attcaaaggt cgtcacacca ccacatcaca cagagaacag agcagcgaac gcgccggact
     6121 agcgggtcgc gacgggctga gcttcgcgag cggcgaggct ctttcttcga ttcgccgaaa
     6181 tcgtccgatc gatgatggct ccacagttaa tgcacttcca ggcgtagaaa atgaggaaga
     6241 agtctgagaa ccgttccaac atcatcatgc ctttgcactt cggacattcc atttgctcct
     6301 cctgggtggt gacgtttaca gctgtgtcac ctctcaagca agagctgcac caaaatgtca
     6361 agtctatgtt tttcaactac ttatgtatgt caaccgccat acatgagatg cgtcaggcgt
     6421 caagtctccg gcacaaaaag gtgcaagccg acattttttt gtcgtaggaa gtaggcaatg
     6481 acacccaaaa aaacagggcg cgggattgga aagtggcccg agactgaacg tccgcgcgaa
     6541 cgtttgctcc gtgagggtgc cgaaagcctc tccgacgcgc aattattggc cattctgttg
     6601 cgcgtggggc ggcaggaagc ctccgccgtc aaagtcggga tggaagtact cgatcgtgtc
     6661 ggaggtatct tcggcctgct gcattgcagc gccgaggagc tctgcgccat tcccggagtg
     6721 ggccctgcca aggctgcgca gttgaaggcg gcggtagaag tcgggaaacg cgcggtgtct
     6781 gcgccgttga cgaccgggac gcgcatcagc tcgagcgccg acctgtttaa gcattaccac
     6841 gcacggttgc gggacttgcg gcacgagatt ttcgctgtgg tgctgctgga cgccaagaac
     6901 caagtcattc gcgacgtgac gatttctgaa ggcagtctga cgttgagcat cgtgcatcca
     6961 agggaagtct ttattcccgc catgcgtgcg tctgccgccg gagtcatttt cctccacaat
     7021 catcccagcg gggaccccac gccaagccag gaagaccggg tgttgacggc ccggctggtg
     7081 tctgccggtt ctctcctggg aattcaggtc ctggatcacc tcatcgtggg ggacggtcgc
     7141 tatgtcagtt ttgccgacca ggggtggttg aatggggaag gcgcgagggt atgaactgcg
     7201 gcgacagatc gcgctcgctc ctttcccggc agaattcgat cgaatgtttt gagaaaggat
     7261 ggcatcacct gcacgtgtct ctctaaccag gcccacagcg gccgccgact catcggagga
     7321 ggtcatcatg aacgttcccc tcaccgaatc catcaggaat gtggctgtgg tatcgcatgc
     7381 gggatcgggc aaaacctcgt tagtcgaagc gctggcgtat tgcgcgggca gtctctccgc
     7441 tcccggctcc atcttcgccg gaaccacggt gtcggatttt gagccggaag aaatccatcg
     7501 ccgaacctca ctcaacacca ccgtccttcg ttgctcttat caggatcgag tgctcacgct
     7561 tctggatacc ccgggctcgc ccagcttcat cggtgagacg cgatcggcat tgcgtgcggc
     7621 agacggtgtg gtattggtcg tgagtgcggc atcgggcgtg cgcagcgccc tgcagaggat
     7681 ctggtcgatg gtccgtgact ccgggttacc ctgtgtcgtg ttcgtcaatg atctggacaa
     7741 ggacggcacg gtattcgaca acacggtgga ggatctgacc aaggaattgg agatgactgc
     7801 cgttcccctg atcctgccga tgggcagcgg tgcgcaattg ggcgacgtcg tggatgtgtt
     7861 gcagaatcat gtggtgacgt cgcagccgga ccgtccgaaa tcgcagcagg ggccggtgcc
     7921 gcctgagtgg cgtgaccggg ttgatcaagc caggcgtcgc gtcatggaac tcgtagccga
     7981 gggagacgag gcgctgctcg aacggtattt gaccgacggc gcattgacgg aggaggcgtt
     8041 gctggaggga ctgcaggccg gagttcaacg cgggtcgctg gtgcccatcg tcggaggatc
     8101 cgccttgcga cacatcggtg tgcactcgct cctgcacact ctcgtcgatc tgttgccctc
     8161 accagtcagt cgcgcccggg ttgcaccgct gcagggcaca gggtcgatgg aagacggggg
     8221 accgataaac aggcacccgc tgccgaccga tccattctcc gccgtcgtct tcaagaccct
     8281 catcgatcca ttcatcggcc gtctttctta cgtgcgcgtc tattcgggaa cgctggaagc
     8341 ggataccgct atgtataatt ccacgcggca ggtcagggag cgaggcgggc atctgttctc
     8401 aattttcggc aaaaaatata cgccgatcac gcgtgcgacg gccggagaga tcgtggcgat
     8461 cggcaagctg aaggatacgg tgacgggaga tacgctctgt gacgagcatg cgccgatccg
     8521 atatcccggg attccatttc ccaggccggt ggtgtcgttt gcgatcgagc cgaaatcgaa
     8581 gacagatatc gagaaggtga gcctggggct gcataaggtg atcgaggaag atccgagcct
     8641 ggagttcgtg cgtcatatcg agacgaagga gatggtcctg agcggcatgg gccaattaca
     8701 catcgatgtg gcgttggaaa aactgcatcg taaatacggc gcggacgtga ttgtgcacgc
     8761 gccgaaaatt ccgtatcggg aaacgattcg tagcgtggcc caggcacagg ggaagtataa
     8821 gaagcagacc ggcggccatg gccaatatgg tgattgctgg ttggaagtcg gtccgttgcc
     8881 gcgcggccag ggcttcgagt ttgagaataa aatcgtcggc ggcgccattc cgcgcaactt
     8941 cgtgccggcg gtcgaaaagg gtgtcgtgga ggcgctgcat gaagggccgc ttgccgggta
     9001 tcctgccgtc gatgtgcggg tgaccgtcta cgatggctcc taccatgtgg tggattcatc
     9061 cgagttggcc ttcaaaatcg ccgggtcgat gggagtgaaa aaagcgctgg aagcggccca
     9121 cccgatcttg cttgagccgc tgatgacggt tgaagtcgag gtaccggccg aatgtgtggg
     9181 gacggtcctt ggcgacctga atgcgaggcg aggccggatc gtcatggtcg aggcgaacgg
     9241 ccacattgaa atcgtcaagg ccctggtgcc acaggccgag atgctcaact atgcggcgtc
     9301 actaaattca atgacgggag gacaggggag ctacgcaatg gaatatgctc aatacgagga
     9361 agtgccgcgc gagttggccg gccgcatcat tgaggaacac aagatggagc gccacggggc
     9421 cgtggcgcat tgagcggcgg gatccgccag aaggaaggat tggcgggcta tccctcttac
     9481 cgatcggcgg gcttcaggac gacgtagaat gcccgccctt cgcgcaggac gcgcaagaga
     9541 atcgtgtcgc cgcgtttgac cttggagacg gccaccgtga attctccgac tgtgcctgtg
     9601 tcgacgcggt tgacttcctt gaccagatcg ccttcccgca acccttcggc ctgggccaga
     9661 cttcctgctt ctactttcga gatcagcacg cctctgctct cgttcagtcg aaacttctcc
     9721 gccagacccg cagtcaggtc ttgtacgtcg attcccagct tcaagtccgt cctgctctga
     9781 ggaagagagg ccgtaaccac cgcatcacgg cgttcgctca gggccacgtt cagcgtgagg
     9841 cgtttgccgt cccgcacgac ttcaacatgc gtcgtggagc cggggtctaa cgcggcgacc
     9901 aggcgggaga gtttgttcgg ggtatcgacg agcgcgccat cgactttggt gatgacatcg
     9961 cccggcttga tgccggccag agcggccggg tcccgttcga atacttcgtt caccaggacc
    10021 ccttcgtttt catccacgcc gaatttgttg gccagatccg gtgtcagcgg ttgcagcccg
    10081 acgcccagcc aggcccgcac gacctttccc ttcgagatga gctggttcat cacctgcttg
    10141 gccatgttgg agggaatcgc aaatccgatc ccctgggcaa aattgatgat ggccgtgttg
    10201 atgccgatga catctccccg cagattgaag agcggtccgc cggagttccc tggattgatg
    10261 gaggcgtcgg tctggatgaa gttctcgtac cgtgagagat tgatgttttc ccgtccgatg
    10321 ccgctgacca ccccgagcgt caccgttcga tccagaccga aggggtttcc gaccgccagc
    10381 acccactgac cgaccttcac tccggtggag tcaccgaacc gggcggcggg gagggggtgg
    10441 tcggtcgtga ctttcagaac agccaggtcg gtgtccggat ccttgccgac gacttgtgcg
    10501 aacagtttgg tcttgtcgga gaggcgaacc tcaacttccg tggcatcacc caccacatgg
    10561 ttattcgtaa taatgtgacc gtttggatcg acgatgacgc ctgagccgga acctgtggag
    10621 ttcggaaccc gctcgccgga tccgtcgcgc gacttgccca gggtctgaat cgggaaaatg
    10681 ctcacgaccg acggtttagc ctcctcggcc aagtccgtga tcacggtttg gagttcttcg
    10741 agcatgcgca gcccgggcga ctctttccga gggggtgatg cgatgtcggt agcggccagt
    10801 gtgatcgctg aggtgcccgt cagccacccg gagaaaccga gcgcgagaat gagtgcggtc
    10861 acgaaggaag tctgtgtctt catgctgggc aagctccgga ggccgaatct cacgaacctg
    10921 gttcggtttc agaataacag attctcgggc ggcaacggaa gcgaggaggc ttaggccgat
    10981 ttcgatgcag ccggtcgttg gaaatgcgga agccagtgag ccaggtcgtg gcgaaggccg
    11041 acaagaatca caatgtcgtc gggccggagc gtcatggaat cggagagcgg gagcaggaat
    11101 gggcctcgaa agaccgctac gatgtgggtc gtcgacggca ggtcgaattc accgatcgga
    11161 tggccgatga ccggtgcgcc acgggcgacg agcacgcgtt cgatggccgc gtcacgcaga
    11221 cccagttcgc gttgcatggt gagcaggtcc tcgtgggcca attcgatctt gaggtcacgg
    11281 atatgccgtt cgatctgctg caaggcggtc tgttgcccct tgatgcggtc ggaggctgtg
    11341 cggatggtat tttccagcga cgggagcgtg ccgggagcga ccgccggtat ggcgccggag
    11401 ggtttggttc ccaactctgc gatgcggcga ccgacctgtc gatgaatggc gtgaatctgg
    11461 ttcagaatcg tcgtggcctg accatggagg cgcaggacat ggaccttgcg gttgacgcgt
    11521 tccgcgatgg cgacaatcgt ctcgtagagt gcgctgccgg tgatgaacag ttctttgcgg
    11581 acgcgagcca acagctcaaa agacagcatc ggataaatgt ccgataagat atattatgtc
    11641 aacttgcttg tccgattcga gattccgtcc cactccacaa ccggaccaga ttgtctttgt
    11701 gccttaccca gattaatagg ctgacgacac aggcaaaccc gatgaaagtc caagaccggt
    11761 gaaacagcaa cgccacaatt ggaaacatgg caaaggcgaa cagtgcgccg cctgacgaat
    11821 atttccacag gaatacggcg ccgacccaca aacccatcag ggtcagtccg atccacggag
    11881 cgactcccag ggcagcgcct aatgcggtag ccacaccctt tccacccttg aatcccagaa
    11941 acgccgagtg cagatgaccg atgaccgagg ccagcgcgac ccagagaatg gccgactcct
    12001 gatgcaacaa catcgtgccg acccagccgg ccagccagcc ttttccgata tcgcccagca
    12061 gcgtcaggat tcctgccttt tttccgctca cgcgcaggac gttggtgaag cccacgttcc
    12121 tgctgccggc gctgcgtggg tccacagcgc ccagaaggcg tgacaccacg actccaaaag
    12181 gaatcgatcc aaggagatag ctggaaagga tgaggaaccc gatgagccag ggactgtcca
    12241 tcaatcgttg cccggatagc cggatacgag cgtcggccga atcgtccggt tagagacctt
    12301 tcgctccgac ctgacgccag aagctccaga catattgtgc gcctgacaca tagcccagga
    12361 tgagtgacag atacaaggtg acaatgccgg ccagatgcag attaccgatc acttccacca
    12421 cggtcccttc gagcaccagg aggacgatgg caatgacctg cagggccatt ttgtatttgc
    12481 cggtgacctc ggcggacatg atcaaacctt cggaggccgc gatggcccgg aggccggtga
    12541 cggcgacttc acgcgcaatg atcagaatcg ccaccagcgc gctgacccga tcgacctgta
    12601 ccagcaggat caatgcagac aacacgagca gtttgtcggc aatcgggtct aacagtcgtc
    12661 cgagtttggt gatctggccg gtcttgcgcg cgacgtatcc gtccagtagg tccgtcacgg
    12721 ccgcgacgac gaatacgatg gccgcggcca aggctcgatc cggcgtcggg tcgatgagca
    12781 gcatgacgaa gaccgggatc aagagaatcc ggacgagcgt cagcacgttg ggcagattga
    12841 tgttgctttc ctgccctgcc gaccgcatca gtaccagccc ggcttgtttc cagttttctg
    12901 ccataaatcc ttcgac
//
LOCUS       DAOQRM010000068        12898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3942_length_12898_cov_17.211321, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000068 DAOQRM010000000
VERSION     DAOQRM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3942_length_12898_cov_17.211321"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..569
                     /locus_tag="PKK81_07115"
     CDS             159..569
                     /locus_tag="PKK81_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32681.1"
                     /translation="MNTTILTICLSAVLTLCPAWPDTSHADSSLPNEPGEELLVAQSS
                     DTIIGLLFRDYSLRGNGQVDYRTARHILGISYDDPASEEPDVALFPLFYWYDANQDGQ
                     WEMWVDRDETGRLTDAVRYDWRQGQELITSSKTW"
     gene            659..1864
                     /locus_tag="PKK81_07120"
     CDS             659..1864
                     /locus_tag="PKK81_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV32682.1"
                     /translation="MMDTSPPHSLRRMPTGIWVLGVVSLLMDISSEMIHSLLPLFMVT
                     TLGAGTIAVGIVEGLAESLALVVKVFSGTLSDYLGRRKGLALFGYALGALTKPLFAIA
                     PDIGTLLTARLLDRVGKGVRGAPRDALVADIAPAHLRGAAFGLRQSLDTVGAFLGPLL
                     AVGLMLLWANDFRAVFWVAVIPGMMAVALLLFGIQEPESRQTTTRANPITRDNFARLG
                     SAYWWVVGIGSIFTLARFSEAFLVLRAQQGGIPIAFIPLVMVAMNLVYASSAYPFGKL
                     SDRMNHQTLLMLGLLVLVAADLVLAMNDHWSVIPAGVALWGIHMGMTQGLLATMVADV
                     APTDLRGTAYGCFNLACGLAMLIASLLAGFLWDRLGAAFTFYAGACFSVLAIIGTLSS
                     PMTQHRRTG"
     gene            complement(1861..2721)
                     /locus_tag="PKK81_07125"
     CDS             complement(1861..2721)
                     /locus_tag="PKK81_07125"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNV32683.1"
                     /translation="MMPEQAVTATARVVQRVVLMVVLFAPGTLPGEAAMPRRAAETRS
                     PCAVHHPSDERVAWHCRRLQRGETLETLFGRRWVDVARFNRIDRRHAQPGREIKIPIR
                     LDDVAEFTPLPGRYPAGEQDQQLIVVDLTEQFIGAYEYGLLRLSFPIASGEPENETPV
                     GDYRITAAHRTHRSCLYTIESTPVPYPMRYALRFYVNAQGVSYWIHGRDLPGYPASHG
                     CIGLADEQMQRQYYGVPPTPVLEDAKRLYEWVIGDRADSGTLLPLSNGPRVVILGSLA
                     KRGDAGASMP"
     gene            complement(2908..3342)
                     /locus_tag="PKK81_07130"
     CDS             complement(2908..3342)
                     /locus_tag="PKK81_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV32684.1"
                     /translation="MRRIDLITQACDPKTLTVGQLMQDALFTCTAQTDALTIGRLMTK
                     QNFGGLPVVAEDSSLVGLVTEYDLLQAMIEGRDLRKVFASEIMTTQPLAARENMTLEE
                     VANLFQDRYVTRLPVVRGKQLVGIVARRDLLHGYMKASAYWS"
     gene            complement(3380..3853)
                     /locus_tag="PKK81_07135"
     CDS             complement(3380..3853)
                     /locus_tag="PKK81_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV32685.1"
                     /translation="MVKKRKAPARRSAGAGRRRVAFEDLTVGDIVNKRVQAARLDMKG
                     DRVAKLLLKEGSNVPVVDQAKQLTGVVSEHDLLSALDEGQAWSDQTAKDLMTENPYSV
                     PPETTLSTLIHVLTESDLMSVPVVNAGNRLVGVVTRRDVVRAALRPGVKRRAKGR"
     gene            complement(3897..4430)
                     /locus_tag="PKK81_07140"
     CDS             complement(3897..4430)
                     /locus_tag="PKK81_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32686.1"
                     /translation="MFESRVIAVMSGMVGLSLWLAGVEPVAGGVTTRVAPGGDAIELE
                     IPTEVGTQKETLPLYHSGKIHYFSAGIGQVEREAAYPPFPLKLVFTAGGKPFVTGVEV
                     VLRQSKGATVLTVPREQVTGPWLFIDVPDGAYEVAATLGGQIQQVKGIHVRRGHVTTQ
                     HVRWAEDRSPVLPAQAE"
     gene            4737..5642
                     /locus_tag="PKK81_07145"
     CDS             4737..5642
                     /locus_tag="PKK81_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV32687.1"
                     /translation="MKMMIAVDGSEFAEWSVQMLEAVASRPPDSVTLVHVVDSASLKS
                     AARKHAAVSKQAIAAMTKAGDQILRRFESLAKIALKQATTKPRTAIDTILAHGRVADT
                     ITKLAKQKKADLLVLGSRGLSDAEHYLLGSVSRKVSALAACPVLVVKRPLTALSHVLF
                     AADASKHSQGACRFLCQRFLPESAHVTVLSVVEPAMTELAAKYLSKDQVEQISVPKRQ
                     AAEQVVENLRDRFLAEGCAVTTQVKVDHVTDTIIQQATSGKVDLLVAGSRGLTGSERL
                     QLGSVSETLLKYAPCSVLIVRGWRA"
     gene            5635..8433
                     /locus_tag="PKK81_07150"
     CDS             5635..8433
                     /locus_tag="PKK81_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNV32688.1"
                     /translation="MPELTALEVCRLAPSQVYRALATTPQGLSSDDVRRRTLRYGPNS
                     LQALRGMPLISRFARQFTHFLALLLWVAAGLAFVADALHSGEGMATLGWAILGVILIN
                     AIFAFLQEYKAERAVQALRSMLPAQAWVLRDGQQQQIARSELVPGDVLVLEEGEQIPA
                     DARLTAAVGMRVDNSSLTGESKPQRRSADPITDGHPLDIANLAFAGTTVLSGHGQGVV
                     FATGLNTEFGKIAHLTTTVQTGLSPLQQEIVKVTHVVAGLSLLMGLVFFTIGVGMGLG
                     FWASTIFGIGIIVANVPEGLLPTVTLALAMGSQRMAARKALIKHLASVETLGCTTVIC
                     TDKTGTLTENRMRLDRLYVDDLVVESREGCLFTRNRLITAPESERWRPLFDAIVHCNN
                     AKRTRHPDGRSRTTGDPTETALLEFAADHGLLHRPPLRRMGEIPFDADRKRMTTLHWS
                     EGRLLAFTKGAPESVLPLCTRQQGSSTPAELTVDGRKKVLAQSQAFAQQAYRVLAVAM
                     REVERGVDQLEADTVEQGLTFLGLVAMMDPPHREVPDAIKKCRRAGVRVMMITGDHPL
                     TALAVARKIGLAPETIPTEPGHFVPVIEGAHIDTFSDDQLRQLLTPTTPGEPDPIFAR
                     MAPRHKMRIVSALKEMREVVAVTGDGVNDAPALKTADIGIAMGIAGTDVAKETASMIL
                     LDDNFATIVHAIEEGRAVYTNIRKFVTYVLASNVPEIVPYLGFGLSSMPLALTIPQIL
                     AVDLGTDMVPALALAAEPPEGGVMDDPPRPRTERLLSWDVILRAYAFLGLIEAGIAMG
                     GFFLYLYSQGWSWAAPLDWTSPLYKEATTVTFAGIVAAQVANVFACRSDRISAFRLGW
                     FSNPLILVGVAVELTILLVMTYSPVGHLVLGTASLPAWIFGPLALGAIGLLLADELQK
                     FVRGRMGARLSLQPGRGT"
     gene            8526..9374
                     /locus_tag="PKK81_07155"
     CDS             8526..9374
                     /locus_tag="PKK81_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV32689.1"
                     /translation="MTTVSGQHVSDYMHRQLEVVPQDTSVVTVATQMRTRSVGSVLIE
                     CFDRPHNDCRIAGIVTETDLVAKVLAPGRVPSRTSMADIMSSPLITIAPTRPMVDASH
                     LMQSKNVRHLVVTEGTDVLGIISIRDLVRHFVDADGGPVQALTNVYRPLSVLMKTAIE
                     TIGSDETALEAAQRMADKHIGALFVMEADELVGIITEGDLVRKLLAYQLDPQTLRVGA
                     LMNSPLLDIDINRTIRDASERMSAKRIRHLAVTEHEKVVGVLSIRDLVKMVAVRDRPD
                     FLKRES"
     assembly_gap    9549..9648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9907..10971
                     /locus_tag="PKK81_07160"
     CDS             9907..10971
                     /locus_tag="PKK81_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32690.1"
                     /translation="MTRMATVPADTAQSYRTHPGILACCLVLTLMQGCTGGFHVRAGD
                     DDDDITESHCVPTTSPPTQVVTLPATGTTYRIDGLTKSSRTEPVTPSRFSPRAREIAV
                     IIGVHDLLVESQLLEDEMAGGMEGALLHSLAVRQELSDRLLLALFEANSIAAELECEK
                     ARTEDLASQLEEVRNDIQQGRTIMAILADSISGVFAGTFLLLGSEILSGLIDLSGSVA
                     ALGFGVAALGGDQAYEFKHGRNLMADVWQGGTISRQFPQPVWQYLNGPVRPNGKRSRR
                     DNIIGTWRTRLGKPGSKEEQRLTGLYFGRGGIYRINELRHRGEMLGLLKTHVNLMTQD
                     LNLLFLETLQHRSSRPEQPI"
     gene            11183..11938
                     /locus_tag="PKK81_07165"
     CDS             11183..11938
                     /locus_tag="PKK81_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNV32691.1"
                     /translation="MLDWLANPEIWIALGTLTALEIVLGIDNIIFLSVLVGRLPEHQR
                     AVARQVGLGLAMMARLGLLFSISWVMGLTHPWVTILGHGVSGRDLILVGGGLFLMAKA
                     THEIHNSLEGIEGGETPAAAASLGMVLVQIALLDIVFSLDSVITAVGLVEHVSIMAVA
                     IILAVVVMLMAAKAIGDFVDKHPTIKILALSFLILVGVTLMVEGFDVHVPKGYIYFSM
                     AFSVTVEMLNIRMRKKRAAPVKLHSRYAEERRQ"
     gene            complement(12017..12529)
                     /locus_tag="PKK81_07170"
     CDS             complement(12017..12529)
                     /locus_tag="PKK81_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNV32692.1"
                     /translation="MLTGLMGLALLSGCSSSLPARYIQQAESDVTLTSLMKSPGAYHG
                     KTVILGGVVVDQQQDGQRLWLHVRNRPLDKDYRPHRPIANEGPEAGYYWISVPNAVTL
                     PPKWKQWARVTVVGRVTDPKTAAPSTEPVLNLVFMRGWTMGQAQQGGAREESVGANNL
                     LSVPEGLHGE"
     gene            complement(12573..>12898)
                     /locus_tag="PKK81_07175"
     CDS             complement(12573..>12898)
                     /locus_tag="PKK81_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV32693.1"
                     /translation="GLVIERERLAAHVAERPSAAEARGWLERLELERIAVVEQPLEVV
                     SGSGQRFLRHPLLRGGFLDDAYECFEDQRLAEEVMTRVSLDLESMTPLIAQRCAISGW
                     KRSVS"
BASE COUNT         2481 a         4137 c         3660 g         2520 t
ORIGIN      
        1 gaactgatca gtgcaacgcc ggtgggtttc aaaaaccacc gtttgtcaac agcctactag
       61 gtaggtatcc aggggcctac ttccgtgagc agcgcaaaga ttgagtatgc agagtcatgc
      121 tcgtctcgac cgctcatgcg agtgaaggga gggatcccat gaacacgacg atactgacga
      181 tatgcctgtc cgccgtgctg acattgtgtc cggcctggcc ggacacttct cacgccgatt
      241 cgtcacttcc caatgagcca ggtgaagagt tgttagtcgc acaatcgtcc gacacgatca
      301 tcggattgct ctttcgcgac tactcgcttc gcggcaacgg ccaggtggac taccgtacgg
      361 ctcgccacat cctggggatt tcctatgacg accctgcgtc tgaggaaccc gatgtcgcct
      421 tgtttccatt gttttactgg tatgacgcca atcaggatgg acagtgggag atgtgggtcg
      481 accgcgacga aacgggccgg ctcaccgatg ctgtgcgtta cgactggaga caggggcagg
      541 aactgatcac atcttccaag acctggtgag tgtgacatga caaccctaag tggcttccct
      601 ataatccgtc aggcagcaga aagcccggta gccgtctcac cgcctcgact tgtcggccat
      661 gatggacaca tctccgcccc attcgctccg ccggatgccg accggcatct gggtgctggg
      721 tgttgtcagc ctgctgatgg acatttcctc ggaaatgatt cacagcctgc tgccgctgtt
      781 catggtcacg acactcggcg ccggcacgat cgcggtcggc attgtggagg gtctggccga
      841 gtccctggca ctcgtggtca aagtcttttc cgggacattg agcgactatc tcggtagacg
      901 gaaggggctg gccttattcg gctatgcctt aggtgcattg accaaaccgc tcttcgcgat
      961 cgcgccggac atcggaacgt tgttgaccgc ccgcttattg gatcgagtgg gaaaaggcgt
     1021 gcgaggcgcg ccccgcgatg cgttggtagc ggacatcgcg ccggcgcatc tccgtggcgc
     1081 agcgttcggc ttacgtcaat cgctcgatac ggtgggggcc tttctcggcc ccctgctcgc
     1141 ggtcggattg atgctactct gggccaacga tttccgggct gtcttctggg tggccgtcat
     1201 accgggaatg atggccgtcg cgctgctgct attcggcata caggagcccg agtcccgcca
     1261 gacgacaaca agagccaatc cgatcacccg ggacaatttc gcacgcttag gctcggccta
     1321 ttggtgggtc gtggggatcg gctccatctt tacgttagcg cgctttagcg aagcctttct
     1381 cgtcctgcgc gcccaacaag gcgggattcc catcgctttc attcccctcg tcatggtcgc
     1441 catgaacctg gtgtatgcgt cctcggccta tccgttcggc aagctctccg acagaatgaa
     1501 ccaccagacg ctgcttatgc tcggtctgct ggtgctggtt gcggccgatc tggtcctggc
     1561 catgaacgat cattggagcg tgattccggc aggcgtggcg ctgtggggca ttcacatggg
     1621 gatgacacaa ggcctgctgg ccaccatggt cgcagacgtg gcaccgaccg atctgcgcgg
     1681 gacagcctac ggctgcttca accttgcctg cgggctcgcc atgctcatcg ccagcctcct
     1741 cgcaggattc ttatgggatc gactcggcgc ggcattcaca ttctacgcgg gcgcgtgctt
     1801 ctccgtgctc gcgatcatcg ggaccctgtc ctctcccatg acacaacacc ggcgcaccgg
     1861 ttaaggcatg gacgcacccg catcccctcg cttcgcgaga ctaccgagta tgaccacgcg
     1921 cgggccgttc gacaggggaa ggagagtgcc tgaatccgct cgatctccga tcacccactc
     1981 atacaatcgc ttggcatcct ccagtaccgg ggtaggcggc acaccgtaat actggcgctg
     2041 catctgctca tcagccagac cgatgcatcc gtgagaggcc ggatatcccg gcaagtcacg
     2101 accgtgaatc caataactca ccccctgtgc gttcacgtaa aaccgcaacg cgtatcgcat
     2161 cgggtagggc acgggcgtcg attcgatggt atagaggcag gatcgatgag tacggtgggc
     2221 agcggtaatc cgatagtctc cgacaggagt ttcgttctcc ggctctccgg aagcgatcgg
     2281 aaaggacaat ctcaacaacc cgtattcata ggcaccgatg aactgttccg tcagatccac
     2341 gacaatgagt tgttgatcct gttctccggc aggatagcgg cccggcaacg gggtaaactc
     2401 cgccacgtcg tcgagccgaa tcggtatttt gatctcccgt cccggctgcg catgccggcg
     2461 atcgatccgg ttgaatcgcg cgacatcgac ccaccgccga ccgaacaacg tctccagtgt
     2521 ttccccacgt tgtagtcgcc ggcaatgcca ggcgacccgc tcatcgctcg ggtgatgaac
     2581 ggcacagggg gaacgggttt cggccgcccg tcttggcata gccgcctccc cgggcaacgt
     2641 gccgggagcg aacaacacaa ccatgaggac gacccgctgt actactcgcg cagtagcggt
     2701 cacagcctgt tccggcatca tgctaggagc ctgtccgaca tggccttcgt agcgaggcca
     2761 aggaggcgcg accagatgcg aggccgcagg cctggaaaaa ccggaagcgt attcgctgaa
     2821 atacgttgag gattttttcg ggccgagaac gacgcagatg atcgtggatc gtttgccgca
     2881 gccgaacggt caatgtcgga caggctccta ggaccaatat gccgaggcct tcatataccc
     2941 gtgcaacaag tcccgcctgg caacgatgcc gacgagctgc ttgccccgca ccaccggcag
     3001 gcgcgtcacg taccggtctt gaaacaggtt cgcgacttcc tccaacgtca tgttttcccg
     3061 agccgccagg ggctgcgtcg tcatgatttc tgaagcgaac actttgcgca aatcccggcc
     3121 ttcgatcatg gcttgcagga gatcgtattc ggtcacgagc ccgaccagag agctgtcctc
     3181 tgccactacg ggcaagccgc cgaaattctg tttggtcatc agccgaccga tggtcagcgc
     3241 gtccgtctgt gccgtacagg taaacaacgc atcctgcatc agttggccga ccgtcagcgt
     3301 tttcgggtca caggcctgag tgatcaagtc gatacggcgc atacgtcgcc ccctctccac
     3361 acacgcaata ggtcaattcc tacctgcctt tcgcccgtcg cttcactccg ggacggagcg
     3421 cggcacgcac cacatcacga cgcgtcacca ctcccacgag ccgattgccc gcattcacga
     3481 ccggcaccga catgagatcg ctctccgtca gaacatgtat cagggtagac agcgtggttt
     3541 ccggcggcac cgagtaggga ttttcggtca tgagatcttt cgccgtctga tcactccagg
     3601 cctgcccctc atcgagcgcc gacaacagat catgttcgct cacaaccccc gtgagctgtt
     3661 tcgcctgatc cacgacgggc acgttgcttc cctctttcag aagcaacttc gccacgcggt
     3721 cgcctttcat gtcgagacgc gccgcctgca cgcgcttgtt cacaatgtcg ccgaccgtga
     3781 ggtcttcgaa tgccacgcgt ctgcgacccg caccggccga tcgtctcgcc ggggctttcc
     3841 gcttcttcac cataccgctt cctccttgtc tacccatgct atccgcagct atcccatcac
     3901 tctgcctgcg caggcaggac aggactccgg tcctcggccc agcgaacatg ctgcgtggtg
     3961 acatgtcctc gccggacgtg aatccccttc acctgttgta tctggccgcc cagcgtggcc
     4021 gccacctcgt aggcaccatc cggcacatcg ataaagagcc aggggcccgt cacctgctcc
     4081 cggggaaccg tcagcaccgt cgcacccttg ctctggcgaa gcacgacctc cacccccgtc
     4141 acgaaaggct tcccgcctgc tgtaaacacc agcttcaagg gaaacggagg gtacgccgcc
     4201 tcacgctcaa cctggccgat gccggcgctg aaatagtgga tcttcccgct gtggtaaagc
     4261 gggagggttt ctttctgcgt tccgacctcg gtagggatct ccagttcgat cgcgtcaccg
     4321 cccggagcca cacgtgtcgt cactcccccg gcgaccggct ccactccggc cagccacaga
     4381 ctcagtccca ccattccact catcaccgca atgactcggc tctcaaacat tgcgcgtccc
     4441 tccttcggac cttctcacgc aatcgtgatg cctgaacgcc gaccgatcag ccaacgtaca
     4501 caggtgcatg tcctccggac cgtcgcgatt cagatctccc ctctcactga tgccaattac
     4561 cccaatatcg tcccgcccac tgctgcaaaa cgccgcaact gcctacgacg actgcccggc
     4621 aaacctgcca accgcctggc atcgcacgca attaccgctt cgtgatcaga ccgcattcag
     4681 gcatacccga tgcaaacctc atcccggagc ggtactgcaa cctgggagaa acacacatga
     4741 agatgatgat cgcggtggat ggatcggagt ttgccgagtg gagcgtgcag atgctggaag
     4801 cggtcgccag ccgcccgccc gactccgtca cgttggtcca tgtcgtggac agcgcatcgt
     4861 tgaaatccgc cgcccgtaaa cacgctgctg tctcgaaaca ggccatcgcc gccatgacga
     4921 aggccggcga ccagattctg cggcgcttcg aaagtctggc gaaaatcgcg ttgaagcagg
     4981 cgaccacgaa gcctcgcaca gccatcgaca cgatcctcgc gcatggccgc gtagccgata
     5041 ccatcacgaa gctggccaag cagaaaaagg ccgatctgct cgtccttggc tcgcgcggcc
     5101 tgagcgacgc agaacattac ctcctgggca gtgtgtcccg taaagtcagc gccttggctg
     5161 cctgcccggt gttggtggta aaacggccgc tcacagccct ctctcacgtg ctctttgcgg
     5221 cggatgcctc gaaacactcc caaggcgcct gccgctttct gtgccagcgc tttcttccgg
     5281 aatccgcgca cgtcacggtg ttgtcggtcg ttgaaccggc catgacggaa ctagcggcca
     5341 agtacctgtc gaaggaccag gtggagcaga tctcggtccc gaaacgtcag gccgccgaac
     5401 aggtcgtaga gaacttacgt gatcggttcc tggccgaagg ttgcgccgtc acgacgcagg
     5461 tgaaggtcga tcatgtgacc gacacgatca tccaacaggc cacctccggc aaggtggatc
     5521 tgctcgtggc aggctcacga ggcttgacgg ggtccgaacg gcttcagttg ggcagcgttt
     5581 cggaaacgct gctgaagtat gccccctgtt cggtgctcat cgtacgagga tggcgtgcct
     5641 gaactgactg cgctagaagt ctgccgcctg gctccgagcc aggtgtatcg cgcgctcgcc
     5701 accaccccgc agggcctctc gtcggacgac gtccggcgac gaaccctccg gtacgggccg
     5761 aatagtctgc aggcattgcg cgggatgccg ctcatcagcc gctttgcgcg tcagttcacg
     5821 catttcctgg cgctgctcct gtgggtggcg gcaggcctgg cgttcgttgc cgatgccctc
     5881 cactccggcg aaggcatggc gacgctaggg tgggccatcc tcggcgtgat tctcatcaat
     5941 gccattttcg ccttcctgca agagtataag gccgaacgcg cggttcaggc tttacgcagc
     6001 atgctgccgg cccaagcctg ggtccttcgc gacggacaac agcaacagat tgcccgcagc
     6061 gaactggtac cgggcgatgt gctcgtgctg gaagagggtg agcagattcc tgccgatgcg
     6121 agactcaccg cagccgtcgg catgcgcgtc gataactcct ctctcaccgg cgaatcgaaa
     6181 ccgcaacgac ggtcggccga tcccatcacc gacggccatc cgctcgacat tgcgaatctg
     6241 gccttcgccg gaactacggt cctctccggc catggccagg gcgtggtctt tgccaccggc
     6301 ctcaacaccg aattcggcaa aatcgcgcat ctgacgacaa cggtacaaac cgggttgagc
     6361 ccgctccaac aggaaatcgt caaggtgacg catgtcgtcg ccgggctctc actgctgatg
     6421 ggcctggtct tcttcaccat cggcgtcggg atgggattgg gattctgggc gagcacgatt
     6481 ttcggcatcg gcatcatcgt cgccaatgtg cccgaagggc ttctgcccac cgtcaccctg
     6541 gccctcgcca tgggcagcca acgcatggcc gcgcgcaaag cgctcatcaa acacctcgcg
     6601 tccgttgaga cactggggtg caccaccgtg atctgcaccg acaagaccgg gacactgacg
     6661 gaaaaccgga tgcggctcga caggctctac gtggacgacc tcgtcgtcga atcgcgagaa
     6721 ggctgtttgt tcaccaggaa tcgcctcatc accgctcccg aatcggaacg ctggcgtccc
     6781 ctgttcgacg ccatcgttca ttgcaacaac gccaagcgca cgcgccaccc ggacgggcgt
     6841 agccgcacca ccggcgatcc cactgagacg gcgctgctgg agtttgccgc ggaccacgga
     6901 ctcctccacc gcccgccgct ccggcgaatg ggcgaaatcc ccttcgatgc ggatcgcaaa
     6961 cggatgacga cgctgcactg gagcgaaggt cggctgctcg ccttcaccaa aggcgcgccg
     7021 gaatcggtct tgccgttgtg caccaggcag cagggatcgt cgacacccgc cgagctcacc
     7081 gtcgacggcc gcaagaaagt gctggcccag agtcaggcct tcgctcagca ggcgtaccgg
     7141 gtgctggccg tggccatgcg agaagtcgaa cgcggcgtgg accagttaga ggccgacacg
     7201 gtagaacagg gactgacgtt tctcggactc gtcgccatga tggacccgcc acaccgcgaa
     7261 gtaccggacg cgatcaaaaa atgccggcgg gccggggtcc gcgtcatgat gattacggga
     7321 gaccatcccc tgacggcatt ggccgtcgca cgcaagatcg ggctggctcc ggaaacgata
     7381 cccacggaac cgggccattt cgtcccggtg atcgaaggcg cacacatcga cacattcagc
     7441 gacgatcagc tgcgccaact gctcaccccg accactcccg gcgaaccgga tccgatcttt
     7501 gcccgcatgg cgccccgaca caaaatgcgc atcgtctcgg cgctcaagga aatgcgcgaa
     7561 gtagtcgccg tgacgggcga cggggtcaat gacgcgccgg cattgaaaac tgccgacatc
     7621 ggcatcgcca tgggtatcgc cggaacggac gtagccaaag agacggcctc catgattctg
     7681 ctcgacgaca atttcgccac cattgtccac gccatcgaag aaggacgggc cgtttatacc
     7741 aatattcgca agttcgtcac ctatgtgttg gccagcaacg tcccggagat cgtgccgtat
     7801 ctgggattcg gactctcgtc gatgccgctc gccctcacga tcccgcaaat tctggccgtc
     7861 gatttgggaa cagacatggt cccggcactg gccctggccg cagagccgcc ggagggcggg
     7921 gtgatggacg accccccgcg accccgaacc gaacgactcc ttagctggga tgtgattttg
     7981 agagcgtatg cctttctggg attaatcgaa gccggcattg ccatgggagg gttttttctc
     8041 tatttgtata gccaggggtg gagctgggca gcccccctcg actggacctc gcccttgtat
     8101 aaggaggcca cgaccgtcac ctttgccggg atcgtggcgg cacaggtcgc aaatgtcttt
     8161 gcctgccgct ccgaccgcat ctcggcgttc cggctcggct ggttcagcaa tcccttgatc
     8221 ctggtgggtg tcgccgtcga actgaccatc ctgctcgtca tgacctacag ccctgtcgga
     8281 catctggtct tgggcaccgc ctcgctcccg gcctggatct tcggaccgct ggcgctcggg
     8341 gccatcggct tgctcctggc agacgaactt cagaaattcg tccgcgggcg aatgggcgct
     8401 cgactctctc tgcaacccgg ccgggggacg tgacatatgc ctggatcgat tgagaccgat
     8461 gagagacaat cgtgcacgtt ctcaatgcca accggcgaca ttcatgttga agaaaaggag
     8521 cttttatgac gaccgtatcg ggccagcacg tcagcgacta tatgcaccgc caattggagg
     8581 tcgtcccgca ggatacctcc gtcgtgaccg tcgccaccca aatgcggacg cgaagcgtcg
     8641 gttctgtgct gatcgaatgc ttcgatcgtc cgcacaacga ttgccggatt gcgggaatcg
     8701 tcaccgaaac cgatctcgtc gccaaggtac tggcgccggg ccgcgttccc tctcgaacca
     8761 gcatggcgga catcatgagc agtccgctca tcacgatcgc gccgactcgt ccgatggtcg
     8821 atgccagtca tctgatgcaa agtaaaaacg tgcggcacct cgtggtgacc gaaggcacgg
     8881 atgtgctcgg aatcatctcg atacgcgatc tggtgaggca cttcgtcgat gcagacggcg
     8941 ggccggtgca ggcgctcacc aatgtctacc gcccgttgag tgtcctgatg aaaactgcca
     9001 tcgaaaccat cggcagcgac gaaacggccc tcgaggcggc gcaacgcatg gcggacaaac
     9061 acatcggggc gctgttcgtc atggaagccg atgagcttgt gggcatcatc accgaaggcg
     9121 acctggtgcg caagctgctg gcctatcaac tcgatccgca gaccctgcgg gtcggtgcgc
     9181 tcatgaattc gccgttgctc gacatcgaca tcaatcgcac catccgcgat gccagtgaac
     9241 ggatgtccgc gaaacgaatc cgccacctgg ctgtcacgga acatgagaag gtcgtaggag
     9301 tcctgtcgat ccgcgacctg gtgaagatgg tcgccgtccg cgatcgcccg gactttctga
     9361 agcgtgaatc gtgaggcgta tctcgtcggg gtcagagcgt atggctggtg gcgtatagct
     9421 gagagtagag acgcccactc tcgattttag gccatacgct aagagccata tgccatacac
     9481 tcttatctgc ggacgtgctc cgtcatcgct cagccctgcc cggtcctgcc gccatcgaga
     9541 tctgcgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg tgctccgtca
     9661 tcgctcagcc ctgcccggtc ctgccgccat cgagatctgc gccgtccggt ccggttccgg
     9721 attcatcgac cgttcatccg cccttcatgc tcgcttcatt tttctccggt accgtcgatg
     9781 acaggatatc tgcggccttc aggctgggag agaacctatc tgcgcgggag ctcatgtctt
     9841 gttgccggaa cgcagagggt cccgggcggg caggcgcagt gctgactgca tcggcagaca
     9901 ccgttcatga cgcgaatggc taccgtgccg gcggacacag ctcagtcata tcgaacccac
     9961 cccggcatcc tcgcttgctg tctggtgctc accctgatgc agggctgcac cggcggattt
    10021 catgtccggg caggcgatga cgatgacgac atcaccgaaa gccattgcgt ccccacgacc
    10081 agtccgccga cccaagtcgt gaccctgccg gccacgggca caacctatcg aatcgacggc
    10141 ctcacgaaat cctcgcgcac cgaacccgtc accccttcac gattctcacc acgggcaagg
    10201 gaaatcgccg tcatcatcgg cgtccatgac ctcctcgtgg aatcccaatt gctggaagat
    10261 gagatggcgg gaggaatgga aggcgcattg ctacactccc tagcggtacg acaggaattg
    10321 tccgatcgcc tgctcctcgc gctcttcgaa gccaacagca tcgccgccga attggaatgc
    10381 gagaaggcaa ggaccgagga tctggcgtcg cagctggagg aagtccgaaa cgacatccag
    10441 cagggacgga caatcatggc cattctggcc gactccattt ccggtgtctt tgcgggaacg
    10501 ttcctgctgc tggggtcgga gattctctcc ggcctgatcg atctgtccgg aagcgtggct
    10561 gcgttggggt tcggcgtggc cgcgttgggc ggcgaccagg cctatgagtt caagcacggg
    10621 cggaatctca tggcagacgt gtggcaaggc ggaaccatca gtcggcagtt cccgcagccg
    10681 gtctggcagt atctgaacgg gccggtccgc ccgaacggta aacggagccg tcgagacaat
    10741 atcattggga cctggcgcac ccgtcttggc aaaccgggct cgaaagaaga gcaacgtctg
    10801 accggactgt atttcggcag aggcgggatc tatcgaatca acgagctgcg gcatcgcggc
    10861 gagatgctgg gcctgctcaa aacccacgtc aacctgatga cccaggacct gaacctgttg
    10921 ttcctcgaaa cgctccaaca ccgctcttcc cgtcccgaac agccgatcta gccccgacgg
    10981 ctccgattct tcctgaagga acggccgttc ccatagccac ctccgcggtc gatccattcg
    11041 ctcacccaac accgcctatc gactggtttt cattgcgtag acccgcttgg aacggtagac
    11101 ttcctcgata cgtatcgtat gctacacatc ctcgcatgac agatcgaccc gctcgccctc
    11161 tctttcacga atggagccgc tgatgttgga ctggcttgcc aatcctgaaa tatggatcgc
    11221 cctgggaacc ttgaccgcgc tggaaatcgt gctggggatc gacaacatta tctttctctc
    11281 cgtcctcgtc ggacgactcc ccgagcatca gcgcgccgtc gcacgccaag tgggcctggg
    11341 cctggccatg atggcgcgcc tgggactctt gttttccatt tcctgggtca tgggactcac
    11401 ccatccctgg gtgacgatct tgggccacgg cgtgtccggg cgggatctga ttctagtcgg
    11461 cggcggcctg ttcctcatgg ccaaagccac gcacgaaatc cacaacagcc tggaggggat
    11521 cgaaggcggc gagaccccgg ccgccgctgc cagcctgggg atggtgcttg ttcagatcgc
    11581 gctcctggat atcgtctttt cgttggattc ggtgattacg gcggtcggcc tggtcgaaca
    11641 tgtctcgatc atggcggtcg caatcattct cgccgtggtg gtgatgttga tggcggccaa
    11701 agccatcggg gattttgtgg ataaacaccc cacgatcaaa attctggccc tctcgtttct
    11761 gattctcgtg ggcgtgacct tgatggtcga agggttcgac gtgcatgtgc cgaagggcta
    11821 tatctacttc tccatggcct tctccgtcac ggtcgaaatg ctcaacattc gcatgcgaaa
    11881 aaagcgggct gcgccggtca aactccacag tcgttatgcg gaagagcggc ggcaataaca
    11941 ctgccggtcg gccggggacg gtgaatcggc cccggcatcg gcaggtggag gcgacgcctc
    12001 gaagcgtgcg ggatcctcac tccccgtgaa ggccctccgg cacagaaagc agattgttcg
    12061 cacccaccga ttcctcccgc gcgccgcctt gctgagcctg ccccatggtc caccctcgca
    12121 tgaataccaa gttgagcacc ggttcggttg aaggcgcggc cgtcttcgga tccgtgaccc
    12181 gcccgaccac cgtcacccgc gcccactgct tccatttggg cggcaatgtc acggcgttcg
    12241 gcaccgagat ccagtagtat cctgcctccg gcccttcgtt ggcgatcgga cgatgaggcc
    12301 ggtagtcctt gtccagcggg cgatttctga catggagcca caaacgctgg ccgtcctgct
    12361 gctgatccac cacgacccct cccagaatga ctgtcttacc gtgataggct cccggcgact
    12421 tcatgaggga agtcaacgtg acgtcggatt ccgcctgctg aatgtatcga gcaggcaagg
    12481 acgagctgca tcccgacaac aacgcaagac ccataagtcc cgtcagcatt cgattgatcc
    12541 gtaccttcac atcaacctcc cgcttttcga cactatgata cgctacgctt ccaaccactg
    12601 atggcacatc gctgtgcaat caacggggtc atactttcca gatccagtga gacgcgcgtc
    12661 atcacctcct ccgccagccg ctgatcctcg aaacattcat aggcatcgtc gagaaacccg
    12721 cctcggagga gcggatgccg gagaaaccgt tggccggacc cgctgacaac ctccaacggt
    12781 tgctcgacga cggcgatccg ctccagttca agccgctcca gccatccacg cgcttctgcc
    12841 gccgatggcc gctccgccac atgggctgcc aatcgttccc gctcgatgac aagtccgt
//
LOCUS       DAOQRM010000069        12848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3962_length_12848_cov_16.867037, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000069 DAOQRM010000000
VERSION     DAOQRM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12848
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3962_length_12848_cov_16.867037"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PKK81_07180"
     CDS             complement(<1..746)
                     /locus_tag="PKK81_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32694.1"
                     /translation="MPPDSLVHEILRRPGTLTFPSNLTDLPHLTAVAGSVSQQPTGHL
                     VVYGSHGRRLCATDPDGHPLHECEWGPVDGALRLLRARVHLDWGAWVGLTPSGLVNTM
                     TLDLSRKPGWQRLRADDLRGMAAQAMRVPLEEVRFFYNDQDVTIGATGAATIRHRKDA
                     IYFLPDGTFDSKQFMACMGAMHWEEIDFLPVVELFLSLLPGTGSALFELIRGLYDDQN
                     RTKPAPRALRYRGIPTYPSEAAYRLFSNFFS"
     gene            complement(747..2060)
                     /locus_tag="PKK81_07185"
     CDS             complement(747..2060)
                     /locus_tag="PKK81_07185"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNV32695.1"
                     /translation="MEQDLTESPVPELDAPVEVLTPEEYVATLVKRAKGAAGRLSSLP
                     TAVKNQALEAMADGLEEHETELLAENEKDLEQFEATPERKALGDRLRLTAERIREMAA
                     GIRDIARLPDPLGDTPKMWTRPNGMQVGRMRVPIGVIGIIYEARPNVTADSAALCLKS
                     GNVCILRGGSEALHSNVAIAKVLSESAEKAGVPAGAITFVDRAEREVVQLLLKQDRYI
                     DLIIPRGGESLMKTIAEHATIPVLKHDKGVCHTYVDVDADPQAAERICLNAKVQRPST
                     CNAMETLLVHHSIARTWLPLLVEKLKAAKVDVRGCAKTCQLCPEVTPAAEDDFGREFL
                     DLILAIKVVKNMEEALEHIATYGSQHTEAIVTKDYARAMRFLREVDAGAVLVNASTRL
                     NDGYQFGLGAEIGISTSRLHARGPMGLEELTCSKFVVLGSGQIRE"
     gene            complement(2185..2769)
                     /locus_tag="PKK81_07190"
     CDS             complement(2185..2769)
                     /locus_tag="PKK81_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV32696.1"
                     /translation="MRGHLRNTDGITYLALMFSIVLIGISVSAAARQWTVMVQREKEA
                     DLMAKGIEIQNAIALYSNTMKIGRILPSEIYPQSLAELTRTPKPYLRRVYQDPMSGGD
                     WEYMRAPTGGIMGVRSKSKAKPIKERDFPLAVRHFEGRKTYHDWMFQHPNPSSMSMLM
                     PPMMGLAPGTMPMQPGPQGSAPGAPPTPGPARNP"
     gene            complement(2783..3484)
                     /locus_tag="PKK81_07195"
     CDS             complement(2783..3484)
                     /locus_tag="PKK81_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32697.1"
                     /translation="MNRVSKNQGLLLMTLVVLWIGLLVWQFGHFSEQERVPLTHVSGQ
                     RTVGKAPAAAVAAGGNLQVHLERLAAIKGQREATFATPRNIFASLLPQEPVVVPPPAP
                     RRGRARGPAKPPSPAGEPVVEAPQNEEEEPTVEEPKGPTPEEIERLRIATELNQFRYV
                     GFMAVGGGSQKKKNMAVVVKNDEMHVVQVGEVIAGEVLVKAVSMSEITLQDVASKITQ
                     VIPVTDAPGAEGQPN"
     gene            complement(3490..4062)
                     /gene="pilO"
                     /locus_tag="PKK81_07200"
     CDS             complement(3490..4062)
                     /gene="pilO"
                     /locus_tag="PKK81_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNV32698.1"
                     /translation="MMFPGLDRLQLTWRKPYAPILPWVAFVAGLCVLSYGIHALVLGA
                     AEQEQVRLEADWLKARQQLNRHKEAKKAKADLQRVWAVLPLFRDFAPLALGVTEEAKR
                     DQVTLPALSYKTEKTAVPDATKAVLQGTVTGRYEDLRRFLHNLESAEELLFIEDLNLI
                     RSGNVQDQQLTFNIRISTYLRGEHLQSPTP"
     gene            complement(4059..4685)
                     /locus_tag="PKK81_07205"
     CDS             complement(4059..4685)
                     /locus_tag="PKK81_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNV32699.1"
                     /translation="MSLDNLVSAIRDVAVRVGQPAARHGAFAINMSSRYRWYLAPARL
                     VIMLLAGAIGLAMLWDISQAWLVWQDVESMESALNQVQDRDRDLLKEAQQEGLDLSEA
                     ALQVLPKEIEFANHLIEKRSFSWTRFLTELERAIPQRIAVNSIRLDPANATIMLTGSA
                     RALEDVTTLTVTFQDHPQFKEPVLGQHRVMASGLVEFDLSLRYRNRNP"
     gene            complement(4689..5696)
                     /locus_tag="PKK81_07210"
     CDS             complement(4689..5696)
                     /locus_tag="PKK81_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32700.1"
                     /translation="MWDWITSRPQYCLKIGARDLAWAEVHRNWRGRPRYQCVVSALPE
                     GIVRLSPLEQNIISPHELETHIRAIAGSATTQAPGRSSTPGVPRAATIILPDLAVRLA
                     VLRLQDLPWSSDERDALVRWRLGQEQLLSLSGAKVFSQVLSDPGGSGDGPCTVLAVVV
                     QEAVLTQYEAVCEAAGLIPQEVDVASLRMFNLWAHGTGRAARSTADFLWVNATDGGVT
                     ALIFHNGTLVFLRSKLQGGIGSGGALAPGSEQVALNRIVQECADSIYACQQQTPELAI
                     SQAVLIADEAMGAGLRQQLEKGLGVPVQELEWDRVQQYGGGTIAGPRTSAALPAIAGV
                     V"
     gene            complement(5700..7433)
                     /locus_tag="PKK81_07215"
     CDS             complement(5700..7433)
                     /locus_tag="PKK81_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV32701.1"
                     /translation="MLMQRHLTRPSLADVMVREGILPRRTVDQVVARLGGSTTAFGQT
                     LVEEGTISEAQLAQALAAQYGLPYDPLTGFRVDSEFYHTISVKLMRRHPFVPVKDEAG
                     ALTIAISDPQNLLALDELEILLNRPLHYVVSSRSAIQSALERSEGSSQALRELEAEYR
                     SVLVKEDERGEEVLTVDHFGEDQSPVVKLLDTIMLSAMQRRASDIHIEATDRATTVKF
                     RVDGILVSAMDPLDVKLHPPLVSRLKVMSDLDIAERRVPQDGSFRMRLDRKTVDFRVS
                     ILPSVFGESVVIRILDREAITTGVSTLRLDRLGFNPEDLKRFRRAITRPYGMVLVTGP
                     TGSGKTTTLYAAISEMNIQEDKLITIEDPVEYQLPGVVQIPVNEKKGLTFARGLRSIL
                     RHDPDKIMVGEIRDAETAQIAIQSALTGHLVLTTVHANNVFDVIGRFASMGIDSYNFL
                     AALTCVLAQRLIRVICPDCRHQVVLDQALAEESGLDYDEFKSAPFYEGKGCSECHDTG
                     YRGRKCITEFLDLTDEIKEMILADRALSEIRYRAVTDGMITLRQSAVKKVLAGETTLR
                     EINRVTFSEER"
     gene            complement(7501..8715)
                     /locus_tag="PKK81_07220"
     CDS             complement(7501..8715)
                     /locus_tag="PKK81_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV32702.1"
                     /translation="MAVFAYRAARADGTTFEGHIEGEDEHLVRAKLESDGLLVFRLSR
                     RGAGFGVPGLAAGRWGKLPLQDFLVFNQELLALIKAGLPVLRVWDLLIDRTQRAPFRE
                     ALKAVRQDIRGGASASEALGKHSTYFSDLYLATIRAGEQSGNLAEVLQRYIAYLKLMI
                     GLRQKVTKALAYPAFLVVVGVAVVGFLLSYVMPTFISVYGESSANLPTATRMLISVIQ
                     MGEAQLIPVAIVAVVAVVMGRAWYQTSAGRFSVDRNLLKLPLLGMILVEHHTIQLTRT
                     LATVLAGGTPLVEALEIARGAVSNRFVSRGLVSAVNEIREGSTLAAAIERPQILPKLA
                     IEMLSVGEETGSLEPMLRDVAEFYEGDLDVRLSQLTTWIEPVLLLVMGLLVGGIVIIM
                     YLPIFQMAGTIQ"
     gene            complement(8756..9595)
                     /locus_tag="PKK81_07225"
     CDS             complement(8756..9595)
                     /locus_tag="PKK81_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNV32703.1"
                     /translation="MNTMIQQAGPVWRTVVGLRFGCSIVATMSLLFWGGCARVTPSPE
                     STPTDLQVAADSLKTAVREAQRTAAELRTELEEQRKELADAQVARAQLQGMLRETERR
                     LADARQVIELQREELTSAHTERERVAQTVAPLHSRLQQPPNGVRLQRKRASSSPDGAV
                     PAPRDTQEQSAVKPLENEEEEAPQSAPADGEAAPVSDVPQASPIVMPIPSAAEPVRTI
                     VVQGGDTLWRLARRHKVSLEALKSLNGLPTNLIVVGRTLRLPEPRLQQAAMQPVSLKT
                     LVR"
     gene            complement(9600..10007)
                     /locus_tag="PKK81_07230"
     CDS             complement(9600..10007)
                     /locus_tag="PKK81_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV32704.1"
                     /translation="MARNTEIGKRSWPFSSPHGFTIIELMIVVTIVGILATLAVPSYH
                     AAIIKAKEGALRQDLFSLRDVIDQHRADKGKYPETIEALVSAGYLRRVPTDPLTGSTT
                     TWQEMVDEGEGGMVDVFSGSDLVGTNGVPYNQW"
     gene            complement(9982..10488)
                     /locus_tag="PKK81_07235"
     CDS             complement(9982..10488)
                     /locus_tag="PKK81_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV32705.1"
                     /translation="MKQSGVTLLELLVTLTILTILATVALPFTKVSTKRTKEIELRQN
                     LRVIRAAIDTFHLEWSRDGDTLTGPACVKNKLSCKDVTGPYGYPKSLDVLLGVKLTGE
                     QATVRGTTIKRYLRSIPLDPVTGAADWRLRCYRDPASVKDWCGEDVYDVTTHSQELAL
                     DGTKYRDW"
     gene            complement(10485..>10819)
                     /locus_tag="PKK81_07240"
     CDS             complement(10485..>10819)
                     /locus_tag="PKK81_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32706.1"
                     /translation="ATQARGLDTETFTLEFDPKILEFRDATLGEVLGTEAGKAAVTAT
                     PSATEGLIELRLHRSASATKDEGRLLRLTFLAKSPGVSSLRLQMAKHDDLDSPEGTGE
                     AVGVVRVR"
     assembly_gap    10820..10919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10920..12724)
                     /locus_tag="PKK81_07245"
     CDS             complement(<10920..12724)
                     /locus_tag="PKK81_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNV32707.1"
                     /translation="MRSSFRTTHVETRDHWCCRVLISTAGVVCGLLITGCGTFVSPDV
                     KRGDQHLAAGNWEEASVAYRQALKDDPFEPSLQNKYSVARERAAAAHDERGRQLLKDH
                     QLDQAAEEFKRALTIEPTSKEHEAGLTEALRLKEARERYREAERLAQLGRVSEAMAVY
                     MRAVELDPTYKEALEGVARLSAEQHAVDRDDRQKQPVTLQFRNAGLKEVVEALGKAAH
                     VNFVFDKDVRNDPVTISLEDKPFDEALSLVLNSNSLFAQKAGPTLFIISPNTKQKQEQ
                     YQDLMIRTFYLSSAKAKEMVALLKSMLDVKHIHGNEALNTIVIRDQPEKVELAEKIIQ
                     ANDREDSEVLFDVEVLEVNRTVDQTYGLSYPKQIGAALIPPGFTGAIAGDIAQQFTYR
                     NLASLGQDSYLFKLPTNVQLDFFKQVTDAKTLAAPKVRVLNNKKAEVNIGDKQPILLS
                     TTNVLPGQAATGAVPTTSTVTSIEFRDTGVKLTVEPNIHLANELSLKMKIEVIRLGDV
                     VLLQQSPPITQFKFGNRSAETMLNVRDGETIVLGGLLQEEDRRTKVTIPWLGDIPFLG
                     NLLSSFKTQRVTTEVILTITPHIVNSLRLPGPQGQA"
BASE COUNT         2562 a         4122 c         3503 g         2561 t
ORIGIN      
        1 gagaaaaagt tgctgaacag ccggtaggcc gcctcggacg gataggtggg gatgcctcga
       61 taccgcagag cccgtggcgc gggtttcgtc cggttctgat cgtcatacag cccgcgaatc
      121 aattcaaaca gcgccgaccc agtgccgggc agcagcgaga gaaacagctc cactaccgga
      181 agaaagtcga tctcctccca atgcatggct cccatacaag ccatgaattg cttggagtcg
      241 aacgtcccgt ccggcaaaaa atagatcgca tccttccggt gccggatcgt ggcggcgccc
      301 gtcgccccga tggtgacatc ctgatcgttg tagaagaacc ggacttcctc cagcggaacc
      361 cgcatcgctt gcgccgccat gccacgaaga tcgtccgccc tcagtcgttg ccaccccggc
      421 ttgcgcgaca agtccagggt catggtattc accaggccgc tgggagtcag gccgacccag
      481 gccccccagt cgagatgcac ccttgcccgc aggaggcgga gcgcgccatc gacgggcccc
      541 cattcacatt catgcaaggg atgaccgtcc ggatcggtcg cacagagacg gcgaccatgg
      601 cttccataga cgacgagatg gccggtcggt tgttgagaaa cggatcccgc cacagccgtc
      661 aggtgtggca ggtccgtgag attggaagga aaggtgagcg tgccgggacg cctgagaatc
      721 tcgtgtacca aggaatcagg cggcatttac tcgcggattt gaccgctccc gagcaccaca
      781 aattttgaac aggtcaattc ttcaagcccc atcggccctc gagcgtggag ccgcgatgtg
      841 ctgatgccga tttccgcccc caggccgaac tggtagccgt cgttgagcct ggtcgaggca
      901 ttcacgagca ccgcgccggc atcgacctcg cgcagaaacc gcattgcgcg cgcatagtcc
      961 ttggtcacaa tcgcctccgt atgttgggat ccgtaggtgg cgatgtgctc caaggcttct
     1021 tccatgttct tgaccacttt gatcgcgagg atcagatcca gaaattcacg gccgaaatcg
     1081 tcctcagcgg ccggcgtcac ctcaggacag agctgacagg tcttcgcgca accacggacg
     1141 tccaccttcg ccgctttgag tttctccacc aggagcggca gccaggtgcg cgcgatactg
     1201 tggtggacca acagggtctc catggcattg caggtcgacg ggcgctgtac tttcgcgttc
     1261 agacagatcc gctcggccgc ctggggatcg gcatccacat cgacataggt gtggcagact
     1321 cccttgtcgt gtttgagcac cgggatcgtc gcatgttcgg cgatcgtctt catcagcgat
     1381 tccccgcctc tcggaatgat gagatcgata taccggtcct gtttgagcaa cagctggacg
     1441 acctcacgct ccgcacgatc gacgaatgta atcgcgcccg ccggcacgcc ggccttctcc
     1501 gccgattcgc tcaacacctt ggcaatggcc acgttcgaat gcaaggcttc ggatccgcct
     1561 cgcagaatac agacgtttcc ggacttgaga cacagggcgg ccgaatccgc ggtcacattc
     1621 ggacgcgctt cataaatgat cccgatgacc ccgatcggca cacgcatgcg gcccacttgc
     1681 atcccgttcg gccgcgtcca catcttcggc gtatctccga gcgggtccgg caatcgcgca
     1741 atgtcacgaa tccctgccgc catctcccgg atacgttccg ccgtcagccg caaacgatcc
     1801 cccaaggctt ttcgctccgg cgtggcctcg aattgttcga gatccttctc gttctcggcc
     1861 aacagctccg tctcatgttc ttcaagcccg tcggccatgg cttccaaggc ctgattcttg
     1921 accgccgtcg gaaggctgga gagccggccg gccgcaccct tggccctttt gaccagggtc
     1981 gcgacatatt cttccggagt gagtacttca acgggagcat ccagctccgg aaccggggat
     2041 tcggtgagat cttgttccat gtcgtgaatt cctgctgctg gccacacatg aggcccgatg
     2101 cctcagcgta tcggccgatc aagatggtga acgaaggcgc ggcgatctat agccagacag
     2161 gatactgtgg ggttcggagt cgggtcaagg gttgcgagca ggacccggtg tcggcggagc
     2221 acccggagca gacccctgcg gccctggttg catgggcatt gtgccgggag ccaggcccat
     2281 catcggcggc atcaacatgg acatcgatga gggattggga tgttgaaaca tccagtcgtg
     2341 atacgttttc cggccttcga agtgacggac cgcgaggggg aaatcgcgct ccttgatcgg
     2401 tttcgcttta ctcttgctcc ggacgcccat gatgccgccg gtcggcgcgc gcatatactc
     2461 ccaatcaccg ccgctcatcg ggtcttgata gaccctccgc agatacggct tgggcgttcg
     2521 cgtcaactcc gccagggact gaggatagat ttcggagggc aggatgcgcc cgatcttcat
     2581 cgtatttgag taaagggcaa tggcattttg aatttcgatg cccttggcca tgagatcggc
     2641 ttctttctcg cgctgaacca tgacggtcca ttggcgggca gcggcggaca cggagatccc
     2701 gatcaacacg atcgaaaaca tcaaggcgag gtaggtaatc ccgtcagtat ttctgagatg
     2761 gccacgcacc cccatcacct gcctaattgg gttgcccctc agcccctggc gcatccgtga
     2821 ccggaatcac ctgcgtaatt tttgatgcca catcctggag cgtgatctca ctcatcgaga
     2881 ccgcctttac caacacctca ccggcaatca cttcgccgac ctgcaccacg tgcatctcat
     2941 cattcttcac gaccacggcc atgttctttt tcttctgcga gccccctccc accgccatga
     3001 accccacata gcggaactgg ttcaattccg tcgcaatccg cagccgttca atttcttccg
     3061 gtgtcggccc cttcggctct tcgaccgtcg gctcttcctc ctcgttctgc ggtgcttcca
     3121 ccaccggctc ccctgcaggc gacggaggct tggcaggacc gcgagcacga ccgcgacgag
     3181 gggcgggagg cggtaccaca accggctcct ggggaaggag actggcgaaa atattgcgcg
     3241 gagtcgcaaa cgtcgcttcc cgctgcccct taatggcggc cagccgttca agatgcactt
     3301 gtagattgcc gccggcagcg acggccgctg ccggcgcctt gcccaccgtc cgctgcccgc
     3361 tgacatgcgt cagggggact cgttcctgtt cgctgaaatg gccgaattgc cagacgagca
     3421 acccgatcca aaggaccacg agggtcatca gcagcagacc ttgatttttg ctgactcgat
     3481 tcatgtgagt tacggcgtcg gggactgcaa atgctccccg cgcagatagg tcgaaattcg
     3541 gatattgaag gtcaattgct ggtcctgcac attccccgag cgaatcaagt tgaggtcttc
     3601 aatgaacaac agttcctcgg ccgattccag gttgtgcaga aagcggcgca aatcctcata
     3661 ccgaccggtc accgtcccct gcaggacggc tttcgtcgcg tcggggacgg cagtcttctc
     3721 tgtcttatag gagagggcgg gcaacgtcac ctgatcgcgt ttcgcctctt cggtgacacc
     3781 gagggccagc ggcgcaaaat cacggaacaa cggcaacacg gcccacaccc gctgcagatc
     3841 cgccttcgcc ttcttggcct ccttgtgccg attcaactgt tggcgggctt tgagccagtc
     3901 ggcctcaagc cgcacctgct cctgctctgc cgcccccaag accagggcat gaatgccgta
     3961 actcagcaca cacagccccg ccacaaacgc gacccaggga agaatgggtg catagggttt
     4021 ccgccaggtc agttgaagac gatcgagtcc tggaaacatc atgggttgcg attccgatag
     4081 cgtaacgaga gatcaaactc gaccaatccg ctcgccatga cccggtgttg cccgagcaca
     4141 ggttccttga attggggatg atcctgaaac gtcaccgtca atgtcgtcac atcttccaat
     4201 gcccgggcgg aaccggtgag catgatcgtg gcattggcag gatcgagccg aatgctgtta
     4261 accgcgatgc gctgaggaat cgcccgctcc agttccgtca gaaaccgggt ccaggaaaag
     4321 ctgcgcttct cgatcaggtg attggcaaac tcgatctcct tgggcaggac ttgcaaggcg
     4381 gcctccgaca gatccaggcc ttcctgctgc gcctctttca acaggtcccg gtcccggtcc
     4441 tgcacctggt tcagagctga ctccatggat tcgacatctt gccaaaccaa ccaggcctgc
     4501 gaaatatccc acagcatcgc caggccgatc gcccccgcca gtaacatgat caccagcctg
     4561 gctggagcca ggtaccaccg ataccggctg ctcatattga tcgcgaatgc gccgtgccga
     4621 gcagctggtt gcccgacacg caccgcgaca tcccgaatcg ccgacacgag gttgtccaac
     4681 gacatggctt acacgacccc tgcaatcgcc ggcaaggcgg cactcgtccg tggtcccgca
     4741 atggtgcctc caccgtactg ttgcacgcgg tcccattcca gttcctgcac cggcacgccc
     4801 aatcccttct ccaactgctg ccgtaacccg gcgcccatgg cttcatcggc gatcagcacc
     4861 gcttggctga tggccaactc cggcgtctgc tgctgacagg catagataga gtccgcgcac
     4921 tcttgcacga tacgattgag cgccacctgc tcggaacccg gtgccaaggc gccccccgag
     4981 ccgatcccgc cctgcagttt cgaccggaga aagaccaacg tcccgttgtg aaaaatcagc
     5041 gccgtgactc cgccgtccgt ggcattcacc cataaaaagt cagcggtcga acgagccgca
     5101 cgccccgtcc cgtgcgccca taagttgaac attcgcaaac ttgcgacatc cacttcctgt
     5161 ggaatcaacc ctgccgcttc gcagaccgct tcatactgag tcagcacggc ttcttgcacc
     5221 acgaccgcca gcacggtaca gggcccatca ccggatccgc ccggatcgga cagcacctgc
     5281 gaaaacactt tggccccgct aagggacagg agctgctcct gccccaatcg ccaccgcacc
     5341 aacgcatcgc gctcatcgga gctccaggga aggtcctgca atcgtaacac cgccagtcgt
     5401 acggccagat ccggcagaat gatggtcgct gcgcgcggga cgcccggagt ggaagagcga
     5461 ccgggggcct gagtggtggc cgaaccggca atcgcccgga tatgcgtctc cagttcgtgg
     5521 ggagagatga tgttttgttc caacggagac aggcgcacga tcccctcggg caacgcggac
     5581 accacgcact ggtaccgcgg cctgccgcgc caattccgat gcacctccgc ccacgccaga
     5641 tcgcgtgcgc cgattttcag gcagtactgc ggccggctgg tgatccaatc ccacatcgat
     5701 tatcgctctt cgctaaacgt cacgcggttg atttcacgaa gcgtggtctc gccggccagc
     5761 actttcttca cggcggattg ccgcaacgtg atcatgccgt ccgtgaccgc gcggtaccgg
     5821 atctccgaca acgcacgatc ggccagaatc atttccttga tctcgtccgt caggtcgaga
     5881 aattcagtaa tacacttgcg gccccgatag ccggtgtcgt ggcattccga acaccctttc
     5941 ccctcataga acggcgcgct cttgaactca tcgtagtcga ggccggattc ctccgcgagc
     6001 gcctggtcaa ggacaacctg atgccggcaa tcgggacaga tcacccgaat cagccgttgc
     6061 gccagaacgc aggtcaacgc cgccagaaag ttgtaggaat cgatccccat cgaggcgaaa
     6121 cgtccgatca catcgaacac gttattggcg tgcaccgtcg tcaacaccaa atggccggtc
     6181 aaggccgatt gaatggcgat ctgcgccgtt tccgcatcac gaatttcacc gaccatgatc
     6241 ttgtccggat cgtgtcgcag aatcgaccgc aacccacggg cgaacgtcag gcccttcttt
     6301 tcgttcaccg ggatttgcac gacccccggc aactgatact ccaccggatc ttcgatggtg
     6361 atcagtttat cttcctgaat gttcatttcg ctgatcgcgg cgtagagcgt cgtggtcttt
     6421 ccgcttccgg tcggaccggt cacgagcacc atcccgtagg gtctcgtaat ggcgcgacgg
     6481 aaccgcttga ggtcctccgg gttgaaaccg agacgatcca gtcgcagcgt cgatacgccc
     6541 gtggtaatcg cctcgcggtc cagaattcgg atgaccacgg attccccgaa cacgctcggc
     6601 aaaatcgaca cccggaaatc gaccgtcttc cggtcgagcc gcatgcgaaa actgccgtcc
     6661 tgcgggacgc gccgttcggc gatatcgagg tcggacatga ccttgaggcg ggacactaac
     6721 ggtggatgca gcttcacgtc gagcggatcc atcgccgaga ccagaatgcc gtctacgcga
     6781 aacttcaccg tggtggctcg atccgtcgct tcaatgtgaa tatcgctggc ccgtcgctgc
     6841 atggcgctca acatgatcgt gtcgaggagt ttgaccaccg ggctttggtc ttcgccgaag
     6901 tgatcgacgg tcaagacctc ttcgccccgc tcgtcctcct tcaccaggac cgaccggtac
     6961 tccgcctcta attcacgtag cgcctgactg gacccttcgc tccgttccaa tgccgattgg
     7021 atggcgcttc gcgagctcac cacatagtgg agcggacgat tgagcagaat ctccaattca
     7081 tccagcgcca gcaggttctg cgggtcggaa atcgcaatcg tcaacgcgcc ggcctcgtcc
     7141 ttcaccggca cgaaaggatg tcgccgcatc aacttgacgg agatcgtgtg ataaaactcg
     7201 gagtccacgc ggaacccggt gagcgggtca tagggcaatc catactgggc tgctaatgcc
     7261 tgcgccaatt gcgcctcgga aatggtcccc tcttcgacga gcgtctgccc gaaggcggtg
     7321 gtgctgcctc ccaaacgggc aacgacctgg tccacggtcc gtctcggcaa aatgccctcg
     7381 cgcaccatca catcggcgag cgacgggcga gtgagatggc gttgcatcag catattcagt
     7441 ccctccgact tcggcacggg tcacgacgcg tcgggcacgg ggtcccgccg tatcgtcgga
     7501 ttattgaatc gtcccggcca tctggaaaat cggcaaatac ataatgatga cgatgccccc
     7561 gaccaggagc cccatcacca gcaacaacac cggctcaatc caggtggtca actgactgag
     7621 ccgcacgtcc agatcccctt cataaaactc ggccacgtca cgcagcatcg gttccagcga
     7681 gccggtttct tcaccgaccg acaacatttc aatcgcgagc ttgggcagga tctgcggccg
     7741 ctcaatggcc gccgccagcg tgctgccttc gcggatttcg ttcaccgccg acaccaaccc
     7801 gcgcgacaca aagcggttcg agactgcacc gcgcgcaatc tcaagcgctt ccaccagcgg
     7861 cgtgccgccg gctaacaccg tggccagcgt gcgggtcaat tggatggtgt gatgttccac
     7921 aagaatcatc ccgagcagcg gcaatttcaa caggttccta tccacggaga atcgaccggc
     7981 ggaggtttga taccacgccc gtcccatcac caccgccaca accgccacga tcgccaccgg
     8041 aatcaactgc gcctcaccca tctggatcac cgagatgagc atgcgggttg ccgtcggcaa
     8101 attagcggat gattcaccat acaccgagat gaatgtcggc atcacgtaac tcagcagaaa
     8161 ccccaccacc gcaacgccga caaccacgag aaaggccgga taggccagtg ccttggtgac
     8221 tttctgacga aggccgatca tcagtttcag atacgcaatg tagcgttgca acacttccgc
     8281 caggttcccg gactgctcac cggcacgaat ggtggcgagg tacagatccg agaaataggt
     8341 ggaatgcttg cccaaggctt cggaggccga cgccccgccg cgaatatcct gacggacggc
     8401 ttttaacgct tctcgaaacg gggctcgttg ggtgcgatcg atgagcagat cccagacccg
     8461 caagaccggt agccccgctt tgatcagggc caacaactcc tgattgaaca cgaggaaatc
     8521 ttgaagggga agctttcccc aacggcctgc cgccagaccc ggcacaccga accccgctcc
     8581 tcgcctggag agccggaata ccagcaagcc gtccgactca agcttggctc gaaccaggtg
     8641 ctcatcttcg ccttcgatgt gtccctcgaa ggtcgtgccg tccgcgcgag ccgctctgta
     8701 tgcgaatacc gccatgagtt cattcgggtg gacacatcac gcgctgcccc ggtctctatc
     8761 gaacgagggt tttcagcgag accggctgca tagccgcctg ctgcagccga ggctccggca
     8821 atcggagcgt ccgtccaaca acaatgagat tcgtcggcaa accattgagt gatttcagcg
     8881 cttccaggct gactttgtgc cggcgcgcaa gtcgccacaa cgtatcaccg ccctgcacga
     8941 caattgtcct gaccggctcc gcggcggacg ggatcggcat cacaatcggc gaagcctgag
     9001 gaacgtctga gaccggtgca gcctcgccat cggcaggtgc ggattgtggc gcctcctcct
     9061 cttcattctc aagcggcttc acggccgact gttcctgtgt atcccttggg gccggcaccg
     9121 ctccgtccgg gctggatgaa gcacgcttcc gttgaaggcg aaccccgttc ggtggctgct
     9181 ggagtcggct gtgcaacggt gcaaccgtct gcgccacccg ctcccgttcg gtgtgagccg
     9241 aagtcaactc ctcacgctgc aattcgatga cctggcgggc atccgccaat cgtcgttcgg
     9301 tctcccgcaa catgccctgc agttgcgccc gcgccacttg ggcatccgcc aattccttcc
     9361 gctgctcctc aagctccgtg cgcaattcgg ccgccgtgcg ctgcgcttca cgcaccgccg
     9421 tcttgaggga gtccgccgca acctgaagat cggtgggggt cgattccgga cttggcgtga
     9481 cccgcgcgca ccctccccag aacaacagac tcatcgtcgc cactatcgag cacccgaatc
     9541 gtaagcccac gaccgtccgc caaacaggac ccgcttgttg gatcatggta ttcattgcct
     9601 taccattggt tgtagggaac tccgttggtg ccgaccagat cggacccgga aaacacatcg
     9661 accataccgc cttccccttc gtcgaccatt tcctgccagg tcgtcgtcga gccggtcaac
     9721 ggatcggtcg gcacccgccg aagatagccg gccgacacca acgcctcaat ggtctcgggg
     9781 tacttccctt tatcggcacg gtgctggtcg atcacatcgc gcagcgaaaa taaatcctgg
     9841 cgcagcgccc cttctttcgc cttgataatc gccgcatggt aggacggcac ggccaacgtg
     9901 gccaaaattc cgacgatcgt caccacaatc atcaactcga taatcgtaaa cccatgcggt
     9961 gacgagaacg gccatgagcg cttaccaatc tcggtatttc gtgccatcca gtgccagctc
    10021 ttgactatga gtcgtaacat cgtacacatc ttctccgcac cagtccttca cactggcggg
    10081 atctcggtaa caacgcaggc gccaatcggc ggctccggtc acgggatcca agggaatcga
    10141 tcgcaggtat cgcttgatcg tcgtcccgcg caccgtcgcc tgctcccccg tcagcttgac
    10201 tcccaacaat acatccaagg atttcgggta gccatagggc ccggtgacgt ctttgcagct
    10261 caacttgttc ttaacgcatg ccggccccgt cagggtgtct ccgtcacgcg accattccag
    10321 gtgaaacgta tcgatcgcgg cccggatcac cctcaagttc tgccgcagtt cgatctcttt
    10381 cgtccgcttg gtcgaaacct tggtgaacgg cagggcgact gtggcgagga tggtgagaat
    10441 ggtcagggtg acgagcagtt cgagcaacgt gacgccggac tgtttcaccg cactctcacc
    10501 actcccacgg cttcaccggt cccctcggga ctgtcgagat cgtcatgctt ggccatctgc
    10561 aggcgcaggg acgacactcc cggagatttg gcgagaaacg tcaatcgcaa caggcgcccc
    10621 tcatcttttg tggcgcttgc ggaccgatgc aggcgcaatt cgatcaggcc ctcggtagcg
    10681 gatggcgtcg ccgttaccgc agctttaccg gcttcggtcc ccaacacctc gccgagggtg
    10741 gcatcccgaa actcgagaat cttcggatcg aattccagcg taaaggtctc ggtatccaac
    10801 ccgcgggcct gtgtcgccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    10921 cctgcccctg cggacccgga agccgcaacg aattcacgat atgcggggta atcgtcagaa
    10981 tcacttccgt cgtcacccgt tgggtcttga acgaactcaa cagattgccc aggaaaggga
    11041 tgtcaccgag ccagggaatc gtcaccttgg tccgacgatc ttcttcctga agaagtcctc
    11101 ccaggacgat ggtctcgccg tcgcgcacat tcaacatcgt ctcggcggac cggttgccga
    11161 acttgaattg cgtgatcggg ggagattgtt gcaacaagac gacatcgccc aggcgaatta
    11221 cctcgatctt cattttcaac gagagttcat tcgccaggtg gatgttcggc tcaaccgtca
    11281 gtttgacgcc ggtgtcgcga aactcgatcg atgtgacggt tgaagtcgtc ggaacggcgc
    11341 cggttgcggc ctgcccgggc aacacgttcg tggtggagag caggatcggt tgcttgtcac
    11401 cgatattgac ctcggccttc ttgttgttga gcacgcgcac tttcggcgct gccagagtct
    11461 tggcgtcggt gacctgcttg aagaaatcca attgcacatt ggtgggaagc ttgaacagat
    11521 aactgtcctg tcccagactg gccaggttcc ggtacgtgaa ctgctgtgcg atatccccgg
    11581 caatcgctcc cgtaaacccg ggcgggatca gagcagcgcc gatttgtttg ggataagaca
    11641 atccatacgt ctgatccacg gttcgattca cttcgagcac ttccacgtcg aagagcactt
    11701 ctgaatcttc ccgatcgttc gcctggatga tcttctccgc cagttccacc ttttccggct
    11761 gatcccgaat cacgatggta ttcagcgctt cattgccatg aatatgcttc acatccagca
    11821 tggacttcag cagcgccacc atctccttcg ctttggcgga tgacaggtag aatgtccgga
    11881 tcatgaggtc ctgatactgc tcctgcttct gcttggtatt ggggctgatg ataaacagtg
    11941 tggggccggc cttctgagca aacaaactgt tgctgttcaa gaccagcgac aaggcctcat
    12001 cgaacggctt gtcctccaac gagatggtga cgggatcgtt gcgcacatcc ttgtcgaaca
    12061 caaaattgac atgcgccgcc ttccccaagg cctccaccac ttccttcagc ccggcattgc
    12121 ggaattgcaa cgtcaccggc tgcttctgcc ggtcgtcgcg atccacggca tgctgttccg
    12181 ccgacagcct ggccacgcct tcgagggctt ctttataagt gggatcgagt tccacggccc
    12241 gcatatacac cgccatggcc tcactcaccc ggcccaattg cgccagacgc tcggcctcac
    12301 gataccgctc gcgcgcctcc ttgagtcgca aggcttcggt caatccggcc tcatgttcct
    12361 tgcttgtcgg ctcgatcgtc aaggcgcgct tgaattcttc ggcggcctga tcgagctggt
    12421 gatccttcag cagctgccgg cctcgctcat catgcgccgc ggctgcgcgc tcccgggcta
    12481 ccgagtattt gttttgcagt gacggctcga acggatcatc cttgagcgcc tgtcgatagg
    12541 cgacactggc ctcttcccaa tttccagccg cgagatgctg atcgccgcgt ttgacgtcag
    12601 gagagacaaa cgttccacag ccggtgatca ggagaccaca cacaaccccg gccgtcgaga
    12661 tcagaacccg acagcaccaa tggtctctag tctcgacgtg tgtcgtgcgg aaggatgaac
    12721 gcataggtcg gatcaacaag atggtaacct ctttggtata tcaaactgac acagacgcga
    12781 caatcagttt ctcgctttgg acggcaagtg ccatgccatc gtctcgcacc tctttcggca
    12841 gatctgtg
//
LOCUS       DAOQRM010000070        12834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_3974_length_12834_cov_11.843258, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000070 DAOQRM010000000
VERSION     DAOQRM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3974_length_12834_cov_11.843258"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKK81_07250"
     CDS             complement(<1..257)
                     /locus_tag="PKK81_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV32708.1"
                     /translation="MGGRRTVVVGCALVMLVGLFSFPRLLLGSDQTRVKEMIQNNCAG
                     CHRLEGKADSRFNLKAPDLIWAGSKYQRSWLLRYLTGKEAPL"
     gene            complement(294..929)
                     /locus_tag="PKK81_07255"
     CDS             complement(294..929)
                     /locus_tag="PKK81_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV32709.1"
                     /translation="MTQSTAKKTSGFVLAALLCTLLGWVVITAAQEDDRGTIQPAQAR
                     QALTLIHARCAVCHTTDLIVQQRLPADRWTVTVEKMMHWGADLSKDEAAAVLQFVTAR
                     YHSGAPDHLPSIEEELAMIGPAVGPRQGSEGPVSGVVSRGAGLYAHNCQACHGEGASG
                     GAGPKLARNTILKNEGAFWETVLHGRGPMPSWGAVLSHQEIADIHAWLATR"
     gene            complement(926..2107)
                     /locus_tag="PKK81_07260"
     CDS             complement(926..2107)
                     /locus_tag="PKK81_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HNV32710.1"
                     /translation="MTVSRREWLRTVLQGIGIGMVVGPGGTSIATAQGSEAGQGTGPL
                     TVRVTRPFDAETPVREFASWLTPNERFFTRSHFGPPSAETVQPDVWRLTVKGLVKEEL
                     TLNLKDLQQLEAVTLTAVLQCSGNGRAHHRPKVPGVQWERGAVGNAQWTGVRLRDVLQ
                     RAGVKPQGVHVQLQGADRPALATVPLFTRSIPLAKALHPDTLLAYEMNGRPLPLLHGA
                     PLRVITPGWMAESCMKWLTEITVRADETPGYYMQQAYRIPETAVRLSSGLPGSAMVPV
                     EQMPVKSLIAAPAEGDTVRIGPVTIQGVAWAGEAPVVKVEVSCDDGKTWEQARLLGEG
                     QPYAWRQWQVVWNARTPGPTAILCRATDAQGEQQPATSSWNPGGFLWNGWDRLSVTVA
                     A"
     gene            complement(2104..>2560)
                     /locus_tag="PKK81_07265"
     CDS             complement(2104..>2560)
                     /locus_tag="PKK81_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNV32711.1"
                     /translation="RAEEDEMKLSVAYQGGARYDILSDRHRIVTDQPVEDGGADAGMS
                     PVELFVGSVASCVGYFVGQFCARHDISREGLKVDAEWTIAEAPHRVGQIQLAIRLPHR
                     VTPELRERLLKVAHGCTVHQSIVVPAAIAIELNPHGHPATASDLMEEKS"
     assembly_gap    2561..2660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2661..3130)
                     /locus_tag="PKK81_07270"
     CDS             complement(<2661..3130)
                     /locus_tag="PKK81_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNV32712.1"
                     /translation="MKHLLFSAIMIVAFMAGCVDSKETRTGAQSAMSQAEPDRLDALF
                     SHPSPDSIPGGQRGEQIRLGYELVVHTQEFAAPYVGNRLTCANCHLDAGLDPNSASYV
                     GLSRAYPEYRARTGRVVTLADRINECFERSLNGKPIPSDSHKLRAIVDYIEWLS"
     gene            complement(3643..4227)
                     /locus_tag="PKK81_07275"
     CDS             complement(3643..4227)
                     /locus_tag="PKK81_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV32713.1"
                     /translation="MSITQKLAGGLVVAVALLGGWGPSFAGAPSAGSVPVEAVMDYLH
                     SVLEANRTFYTVHVVERMQRRGVIESSEKWRELSALPLPAQFFQEASSLATLTGSAVR
                     YRLISLNPINKQNGPHGEFERKALEAVMAHPEQPYKGSVTEGGVRYFQALYADLAVSS
                     VCVSCHNADPRSPKRDYKMRDVLGAVLISIPISE"
     assembly_gap    4312..4411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4412..4679)
                     /locus_tag="PKK81_07280"
     CDS             complement(<4412..4679)
                     /locus_tag="PKK81_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32714.1"
                     /translation="MMTDVWRSGGLLVALVVGMTTVFAVAVTRVAAKGEQAGEQVVRS
                     TCTGCHRIEGPVAARKTKKAPDLIWAGNKYQPAWLVNWLQNPDFK"
     gene            complement(4716..6041)
                     /locus_tag="PKK81_07285"
     CDS             complement(4716..6041)
                     /locus_tag="PKK81_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNV32715.1"
                     /translation="MSSRLYDWFDRRLNLKPVQRTLLDEPIPGGASWIYVFGSVTLFL
                     FVLQAATGMFLALYYAPTPDHAYDSVRFIETEVTFGWFVRGLHHWGASSMVVAIGLHM
                     LQTFLYGAYKKPREVMWMVGVVLFLVVMTFAFTGYLLPWDQTAYWATQIGINMVGTVP
                     LIGDFLSRVLRGGEILGALTLSRFFAIHVLFLPAILIGGIALHLFVLRRVGPAGPWTD
                     DRASLGSETFAPRQVYMDAVVIALVFLTVAALALSIPLPLTDKANPSDTSFVPVPEWY
                     FLFYYELLKYVHGPLEPLATWVLPLSVILIMLCWPFIDRNPVRNPIRRPVALGSGLLF
                     LFVVFGLLGISLKNLYAVPRTDPAVVQGRAIYAQLGCAGCHRIHGEGGAVAPDLSTIG
                     DARPDRAWHLKHFRDPQSVSPGSFMPKFPLTDQQLNDLTSYMLSLKRES"
     gene            complement(6078..6629)
                     /locus_tag="PKK81_07290"
     CDS             complement(6078..6629)
                     /locus_tag="PKK81_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNV32716.1"
                     /translation="MSYSDDTQSCEGAGLSTPVGSRRTFFHWVTVAAAAVVGVGLAVP
                     LLGSLVSPAFMRRKREWVDVGPVDTLPAGRPTQLDHVTTVRDGWLEAKSQKAVWAVKQ
                     AQGEVKVFSPICTHLGCGYRWDDTEKKFLCPCHGSAYDINGQVLGGPAPRPLDVLPSK
                     VEGGRLLVMYQDFKSGLPQSVEL"
     gene            complement(6719..7162)
                     /locus_tag="PKK81_07295"
     CDS             complement(6719..7162)
                     /locus_tag="PKK81_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV32717.1"
                     /translation="MTTMARPGVPVGGFKTVGQIVGTNQLRIHRNQSALAIAVELLTS
                     HTPGAPVVDDAGQYIGFISEFDLLKALRSNQDLNQLTAEQVMVKDRITVTAETSIDDA
                     VRMMEEKRLLNLPVEKDGTIRYTLTRHDLLRAWIGLGLDIENQTV"
     gene            complement(7268..7486)
                     /locus_tag="PKK81_07300"
     CDS             complement(7268..7486)
                     /locus_tag="PKK81_07300"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNV32718.1"
                     /translation="MACNVGGIERPVRIVLGVLLIGIGVFAGLPPVGMGVALAMGAIA
                     LVTGAIGFCPVWTLLGVNTCPIDAGKKS"
     assembly_gap    7501..7600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7601..7803)
                     /locus_tag="PKK81_07305"
     CDS             complement(<7601..7803)
                     /locus_tag="PKK81_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV32719.1"
                     /translation="MMNARVTGPVLLSMLLIAAVGSGSYAAGGNVAKGKVLYTNLCIR
                     CHGVDGKGDAYMKFNPPVADLSSP"
     gene            complement(8029..8463)
                     /locus_tag="PKK81_07310"
     CDS             complement(8029..8463)
                     /locus_tag="PKK81_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNV32720.1"
                     /translation="MDVRSKVYTYRTAVKWTEQKKGTITCEGKPDIQVATPPEFKGHE
                     GIWSPEDLYVASANICLMTTFWAVAERAGLAFTSYHCEAEGRLELVEGKFQVTVITLR
                     PQIALQSGSDAAKAKELIEKAEANCLISNSMKTRIALEPTIS"
     gene            complement(8510..8926)
                     /gene="rnk"
                     /locus_tag="PKK81_07315"
     CDS             complement(8510..8926)
                     /gene="rnk"
                     /locus_tag="PKK81_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNV32721.1"
                     /translation="MNQRDIYITEFDLTRLRDVLRARISIEARDRDHLESLENELDRA
                     HVVDPSAVPRDVVTMNSQVRIEDVETGMETVYTLVFPSDAGIQEKKLSILAPIGTALL
                     GARAGETVDWPVPAGVRTVRIKDVLYQPEAAGHYHL"
     gene            complement(8923..9900)
                     /locus_tag="PKK81_07320"
     CDS             complement(8923..9900)
                     /locus_tag="PKK81_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV32722.1"
                     /translation="MTKPLMTRILLATDFSDGAACAQDYAIYLASHWGATLEVLHVIE
                     TPHRSSAEAESLAAVVQVRAEAARQLEQVRDDLVRRGVSVKVRLVLGNPAEHIGLAAK
                     DKGAELVVLGVQGRTNLLYGLIGSTAERVVTEGPCPVLAVPGLREEAGRPSAAGRQVH
                     IRHILAPLDFSSPSLDAVEYAIQLANGLGATVTLMHVLEPVCYDLDCGLGVVEQEARK
                     RDHWNRQLSELQTLVTSFGLAADVEISGGIASDAILASALRHRSDLIVMGTHGRRGVS
                     AQRFGSVSEAVLRLATCPVLTVKTPKFAAGHHRVVPQAMRETEIKGAQP"
     gene            complement(11191..11730)
                     /locus_tag="PKK81_07325"
     CDS             complement(11191..11730)
                     /locus_tag="PKK81_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32723.1"
                     /translation="MALDQDVLRSDYAHLLTLWTSGVRDYHTMLSDYLTANSIFVAVI
                     GLLVSRESLALPFTLIIVLFSIIGILMSVQMAIVLGRFSGQNALWEWQLRGIETMPEW
                     RERKPVNSLYRLREHRETIVEDTNEPRFFEPSWAFRQHRQWWAHRAISFPWFFGTVYG
                     LFLLWGLTQVVRASTLSWG"
     gene            complement(11735..>12834)
                     /gene="glgP"
                     /locus_tag="PKK81_07330"
     CDS             complement(11735..>12834)
                     /gene="glgP"
                     /locus_tag="PKK81_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNV32724.1"
                     /translation="VREQCVFTTHTPVPAGHDQFPVELAHRVLGSRRCDLVQACQHQG
                     ALNMTLLALRGSRFVNGVAMRHGEVSHSLYPGYPIHSITNGVHAVTWTAPSFQHLYDR
                     HLPDWRYDQLSLRYAISIPGAEIWRAHQDAKLALVDEVNRQTNAGFDRDVLTIGFARR
                     ATAYKRGTLVFHDVERLRRLLKEAGPIQFVFAGKAHPQDQAGKDMIHRIYGMRGQLSV
                     AYLANYDMTLARLLCAGVDVWLNTPLPPMEASGTSGMKAAMNGVPSLSVLDGWWVEGH
                     IEGVTGWSIGEKIEACVSPAEDMEACHARALYDKLEQRVMPCFYKDREGFIEIMKHAI
                     AVNGAFFNTQRMVGQYLRNAYRLVGQDVNGA"
BASE COUNT         2629 a         4084 c         3420 g         2401 t
ORIGIN      
        1 agcggcgcct ctttccccgt caggtaacga agcaaccacg agcgctgata tttgctcccg
       61 gcccaaatca gatccggcgc cttcaaattg aaacgcgaat ccgctttccc ttccaagcgg
      121 tgacaaccgg cgcaattgtt ctgaatcatc tccttgacgc gagtctggtc actgcctaac
      181 agcaagcggg ggaatgagaa caaccccacc aacatgacca acgcgcatcc gactaccacc
      241 gtccgtcttc cacccatgaa acaccccttt ctttcgcccc actgccgtac cggtcagcgt
      301 gtcgccaacc aggcatgaat atccgcgatc tcctgatgac tcagcactgc gccccaggac
      361 ggcatcggcc cccgcccatg gagcaccgtc tcccaaaaag ccccttcatt tttcagaatc
      421 gtattgcgcg cgagtttcgg tccggcccca ccggacgctc cttcaccatg gcaggcctgg
      481 caattgtgcg catagagtcc cgcgcctctc gataccacac cggacaccgg cccctcagac
      541 ccctgacgcg ggcctaccgc aggcccgatc atggccaact cttcttcaat cgacggcaga
      601 tggtccggcg caccggaatg ataccgcgcc gtcacgaatt gcaagaccgc cgccgcctcg
      661 tccttggaca gatccgcgcc ccaatgcatc atcttctcaa ccgtcactgt ccatcggtcg
      721 gccggcaacc gttgctgcac gatcaggtcg gtcgtgtggc agacggcgca acgggcatga
      781 atcaatgtca gtgcttgacg agcctgggcc ggctgaatcg ttccacggtc atcttcctga
      841 gccgccgtga tcaccaccca gccaagcaga gtacagagca atgcagcgag cacgaaaccg
      901 gaggtctttt tcgccgtgct ctgggtcatg ccgccaccgt gacggacagg cgatcccatc
      961 cgttccatag aaatcctccg ggattccaag agctcgtcgc cggttgctgc tccccctgcg
     1021 catcggtggc gcggcagaga atcgcagtag gcccgggcgt gcgggcgttc cacacaacct
     1081 gccattgtcg ccaggcatag ggctgcccct ctcccaataa ccgggcctgt tcccaggtct
     1141 ttccgtcgtc gcaggacact tcgaccttca ccaccggcgc ctcgccggcc caggcgacac
     1201 cttgaatcgt gaccggcccg atccgtaccg tgtctccctc cgccggagcg gcgatgagcg
     1261 acttgacggg catctgctcg accggaacca tcgcgcttcc cggcaatccc gaagataggc
     1321 gaacggccgt ttccggaatg cgataggcct gctgcatgta gtagccgggc gtttcatccg
     1381 ctcgaaccgt aatctcggtc agccacttca tgcaggactc cgccatccag ccgggagtaa
     1441 tcacccgcag cggcgcgcca tgcagcaggg gcagcggacg gccgttcatt tcataggcca
     1501 ggagggtatc gggatggaga gcctttgcga gggggatact tcgcgtgaac agcggcacgg
     1561 tcgccagcgc cggccggtcc gccccctgca attgcacgtg gactccctgc ggtttcactc
     1621 cggctcgctg caacacatcg cgaagccgca cgccggtcca ttgcgcattg ccgaccgctc
     1681 cgcgttccca ctgcacaccg ggcactttgg gacgatggtg cgcgcgtccg tttccgctgc
     1741 attgcaggac ggcggtcagg gtgacggcct cgagttgttg taagtctttg aggttgagcg
     1801 tcagctcctc tttgaccagc cctttgaccg tcagtcgcca gacatcgggc tgtaccgtct
     1861 cggctgaagg agggccgaag tgactgcgcg tgaaaaatcg ctcattgggt gtgagccagg
     1921 aggcaaattc ccggaccggt gtttctgcgt cgaacggcct ggtcactcgt acggtcaacg
     1981 gcccggttcc ctgccccgcc tcactcccct gcgcggtggc gatcgaggtg cccccaggcc
     2041 cgaccaccat accgatgccg attccttgca gtaccgttcg caaccattcc cgtcgggaca
     2101 ccgtcatgac ttttcctcca taagatcaga tgccgtcgcc ggatggccgt gcggattcag
     2161 ctcgatcgcg atagcggcag gcacgacgat cgattgatgg accgtgcacc cgtgcgccac
     2221 cttcagcagc cgctctctca gctcgggtgt cactcgatgg ggaaggcgaa tcgcaagttg
     2281 gatctgcccg actcgatgcg gcgcttcagc tatcgtccat tcagcgtcca cctttaggcc
     2341 ttcgcgcgag atatcgtgtc gcgcgcagaa ctggccgaca aagtacccca cgcagctcgc
     2401 caccgaaccg acaaacagtt ccacgggact cataccggcg tcggcccctc cgtcttcaac
     2461 cggctgatcc gtcacgatcc ggtgccggtc gctcaggatg tcgtaccgcg ctcccccctg
     2521 atacgccacg ctgagtttca tttcgtcctc ctccgctcgc nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn ttcgacaacc attcgatgta gtccacgatg gcccggagct
     2701 tgtggctgtc cgacggaatc ggcttgccgt tcaagctgcg ctcaaaacat tcattgatac
     2761 ggtcggccaa tgtcacgaca cgcccggttc gcgcgcgata ttccggatag gctcgcgaga
     2821 gcccgacata ggacgccgaa ttgggatcga gccctgcgtc caaatgacaa ttggcgcacg
     2881 tgagccgatt ccctacgtag ggtgcggcga attcctgcgt atgaacgacg agctcatacc
     2941 ccagccggat ctgctccccg cgctggccgc cggggatgga atcgggggag ggatggctga
     3001 agagggcatc gagcctgtcc ggctcggctt gactcatggc agactgtgcg ccggtgcgtg
     3061 tttccttcga atcgacgcaa cctgccatga acgcgacgat catgatggcg ctgaacaata
     3121 ggtgtttcat acgtagatag tctcgcaaac tacataccac gtcgacagaa gctcgcctac
     3181 gagaattagc ttttggcagg ccctcagggg aatgcatgcc tgcatacttg acgacaaaag
     3241 ccacagtccc cctccgccgc ctgcggcatc gtgctacagc gaactcatac accaccggct
     3301 tgaatgtggt gattagggac aggtttgggg aaaaggattc agaatgttga tttgatcgtt
     3361 ctgaggcgtt atctgccctg gttcctcgcg ccggcccggg aggcttcgag gatggacgaa
     3421 gggactaacg tgatcaccgg ccgatataac gtcggtgcct tgcactcatc attcacgctt
     3481 ccgagcatca tgtctttggt gttctcgata atcctacgcc gctacgggga ttccggttcc
     3541 ggccacaaca taggcacggc cctacgcagc ctatgccgta agccgtagcc cccttcgcag
     3601 cgctcggatc acgtcgaaca gcttgatcca tcctgagacg agtcactccg agatcggaat
     3661 cgagatcagg accgcgccga gcacgtcccg catcttgtag tcccgtttcg ggctgcgggg
     3721 atcggcgtta tggcagctca cgcacacgga cgacacggca agatccgcat acagggcctg
     3781 aaagtatcgc acgcctccct cggtcaccga ccccttatac ggctgctctg gatgtgccat
     3841 gaccgcttcg agcgccttgc gctcgaattc gccatgaggc ccgttttgtt tgttgatcgg
     3901 gttcaggctg atcaagcgat accggacggc gctgccggtc agcgtggcca ggctcgatgc
     3961 ctcttgaaag aactgggctg gaagcggaag ggcgctgagt tcgcgccatt tctcagaaga
     4021 ctcgatcacg cccctccgct gcatccgctc cacgacatgg acggtataaa atgtgcgatt
     4081 ggcctccagg acggaatgga gatagtccat caccgcttca accgggacac tgccggccga
     4141 tggtgcgccg gcaaagctgg ggccccagcc tccgagcaga gcaaccgcca caaccagtcc
     4201 ccccgcaagc ttctgtgtga tcgacataac agcctccctt catgatggtc ctccggccaa
     4261 gagacgacca ccgggtacag tcttgtgata ctcttcttcg aaccaccagc annnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcttgaaat cgggattctg gagccagttg
     4441 acgagccacg ctggctgata cttattaccg gcccaaatca ggtccggtgc cttcttggtt
     4501 ttgcgggctg cgaccggacc ttcgattcga tggcagcccg tacaggtgct cctcacaact
     4561 tgttcgcccg cttgctcacc ctttgccgca acgcgagtga ccgcaaccgc aaagaccgtc
     4621 gtcatcccaa ctacgagcgc cacaagaagg ccgccactac gccagacgtc ggtcatcatc
     4681 gctcctttcc gttctctgcc cgcattagac acaccctaac tctcccgctt caaactcagc
     4741 atgtagctgg tcaagtcatt gagctgttgg tcggtaagcg ggaatttcgg cataaacgat
     4801 cccggcgaga cggattgcgg atcgcggaaa tgcttgagat gccacgcccg atccggtctc
     4861 gcgtctccga tcgtagacag gtcgggggcg acggcgcctc cctcaccatg gatgcgatga
     4921 caaccggcac aaccgagctg ggcatagatc gctcgtccct ggacgacggc cggatcggtt
     4981 ctcggcacag cgtacagatt cttgagcgag atgcctaaga gaccgaagac gacgaacagg
     5041 aagagcaggc cgctgcccaa ggccaccggc cgccggatgg gattgcgcac cgggttacga
     5101 tcgatgaacg gccagcacaa catgatcaga atcacagaca gaggaagaac ccaggtcgcc
     5161 aacggctcaa gcggcccatg cacgtacttg agcagctcgt agtaaaacag aaagtaccat
     5221 tcggggaccg gcacgaagct cgtatcggaa ggattagcct tatccgtcag gggcagagga
     5281 atgctcaagg ccagcgcggc gaccgtgaga aacaccaggg caatcaccac cgcatccata
     5341 taaacctgcc ggggagcgaa ggtctcgctg cccagcgacg cgcgatcgtc ggtccagggt
     5401 ccggccggcc ctacccgcct caacacgaac agatgcagag cgatcccgcc gatcaggatg
     5461 gcggggagaa ataacacatg aatcgcaaag aaccgtgaca gcgtcaatgc gccgaggatt
     5521 tctcctcctc gcaagacccg ggagagaaaa tctccgatga gcggaaccgt gcccaccatg
     5581 ttgatgccga tctgagtcgc ccaataggcg gtttgatccc agggcagcaa atagccggta
     5641 aacgcgaagg tcatcaccac gaggaacagc accaccccga ccatccacat cacttcgcgc
     5701 ggctttttgt acgcgccata gagaaaggtc tggagcatat ggagaccgat cgccacgacc
     5761 atcgacgacg cgccccaatg gtggagcccg cgcacgaacc acccgaacgt gacctcggtt
     5821 tcaatgaatc ggacgctgtc gtaggcgtga tccggcgtcg gagcataata aagcgcgagg
     5881 aacatgccgg tggcggcctg aagtacgaac aagaacaacg tgacggagcc gaagacgtaa
     5941 atccagctgg ctccgcccgg aatgggttcg tcgagcagcg tgcgctgcac cggtttcagg
     6001 ttcaaccgac ggtcgaacca atcgtatagg cgagaagaca ttcaactccc agatctcggc
     6061 aatgaaacca tcgccgctca caactccaca ctctgtggaa ggcccgattt aaagtcttga
     6121 tacatgacga ggagccggcc tccctccact ttcgacggga gtacgtccag cggacgcggc
     6181 gcgggtccgc ccaacacctg accgttgatg tcataggcgc tcccgtgaca gggacagagg
     6241 aacttctttt ccgtatcgtc ccagcggtac ccgcatccca gatgcgtgca gatgggcgag
     6301 aagaccttca cctcgccttg cgcctgcttc accgcccata ccgccttctg ggacttcgct
     6361 tccagccagc catcgcggac ggtggtcaca tgatccaatt gggtagggcg ccccgcgggc
     6421 aacgtatcca ccgggccgac atcgacccat tcacgtttgc gtcgcatgaa ggccggtgac
     6481 accagcgagc cgagcagtgg cacagccagc cccaccccga ccacagcggc cgcggcaacc
     6541 gtcacccagt gaaaaaatgt ccgccgcgac ccgaccggag tcgacagccc tgccccttca
     6601 cagctctgtg tgtcgtcgga ataactcacg ttcgtaatcc atcatgtagg gtgattccgg
     6661 gcaggagagg aggcggcagc ctattcactc ccgccccctc cgccattcgc aacttgattc
     6721 agaccgtctg gttctcaata tccagcccca gaccgatcca ggcccgcagc aaatcgtgtc
     6781 gggtcaatgt gtatctgatc gtgccgtcct tctcgacggg cagattgagc aaacgcttct
     6841 cttccatcat tcgaaccgca tcgtcgatcg atgtctccgc cgtcacggta atccgatcct
     6901 tgaccataac ctgttctgcc gtcagctgat tgagatcctg gttcgacctc agggctttca
     6961 atagatcgaa ttcactgatg aacccgatgt actgtcccgc gtcatcgacc accggcgccc
     7021 cgggtgtatg actcgtcagc aactccaccg cgatcgccaa tgcgctttgg ttccggtgaa
     7081 tgcgcaattg gttcgtccct acgatctgtc caaccgtctt aaatccgcct accggcacac
     7141 ccggtctcgc catcgtcgtc atcctgtcct ccttcgcgct actccagtga cggaattgcc
     7201 accggcactc tccacactgc ccatgaccac cctgcgcaca cgccacttca ccgcaaaacc
     7261 tgttgggtca ggatttttta cctgcgtcga tcgggcaggt gttgaccccc aacagagtcc
     7321 ataccggaca aaacccgatg gcgccggtga ccagcgcaat cgctcccatg gccagcgcaa
     7381 cccccatccc cacgggaggc agcccggcga aaacaccgat tccgatcaac aagactccca
     7441 acacgatccg gaccggccgt tcgattccac caacattgca tgccatcaca gcacctcctt
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggagagctga gatcggcgac
     7621 cggaggatta aacttcatat aggcgtcgcc cttcccatcg actccatggc aacggataca
     7681 gagattggta tagagcacct tgcctttggc cacattcccc ccggccgcat acgaaccgct
     7741 gccgaccgcc gcaatgagga gcatcgacag gagaaccgga ccggtgactc tggcgttcat
     7801 catcttctcc tttgtaagga gctgccgcac acccgtggca ccccacatac gacgatgaga
     7861 atgcgatcct cgtgccagtc ggacaggaag ggacaaccaa gtgaaatgag agaaacaggg
     7921 tgagagtaac cgattcgcgg aaaaactcga cgcccagccg ggatggccgt ggagagaatc
     7981 accccaccac tcgatcccgg ctggcgcaag atgccccaat ccggagcgtc aggagatggt
     8041 gggctctagg gcgatacgcg ttttcatcga attcgagatc aggcagttgg cttccgcctt
     8101 ctcgatgagc tccttggctt tcgccgcatc gcttcccgac tgaagcgcaa tctgcggacg
     8161 caacgtaatc acggtgacct gaaactttcc ctctacgagt tccaaccgtc cttccgcctc
     8221 acagtggtac gacgtgaagg ccaatccggc ccgttcggcg accgcccaaa atgtcgtcat
     8281 caggcagata ttggccgacg ccacatacaa gtcctccggc gaccaaatcc cctcatgtcc
     8341 tttgaactcc ggaggtgtcg ccacctggat atccggtttt ccttcgcagg taatcgttcc
     8401 ttttttctgt tccgtccatt tcactgccgt acgatacgta tagaccttgc tgcgaacgtc
     8461 catcgcttcc tccggtatgg ttcatactgt gtggactaca gcctacctgc tataggtgat
     8521 aatgccctgc ggcttcgggt tggtacaaga cgtccttgat gcgcacggtt cgcacgcccg
     8581 ccggcacggg ccagtccacc gtctcacccg cgcgcgcccc caacagagcc gtcccgatcg
     8641 gcgcaaggat cgacagcttc ttctcctgga taccggcatc agaaggaaat accagcgtat
     8701 agacagtttc catgccggtc tcgacgtctt cgatccggac ttgggagttc atcgtgacga
     8761 catcacgggg aacggcggat gggtccacga catgggcgcg atcgagctca ttctccaagc
     8821 tctccagatg atccctgtcc cgcgcctcaa tgctgatacg agccctcagg acatctctca
     8881 ggcgggtcaa atcgaattcg gtaatataga tatcgcgctg gttcatggct gtgctccctt
     8941 aatttccgtc tctctcatcg cttgcggcac gacccggtgg tggcctgcgg cgaatttcgg
     9001 cgtcttgacc gttaagaccg gacaggttgc caggcgcaac accgcctccg acacgcttcc
     9061 gaaccgttgc gcggagacac cgcggcggcc atgggtcccc atcacgatca gatcggatcg
     9121 atggcgtaac gcagaggcga gaatcgcatc ggaggcaatg cctcctgaaa tctcgacgtc
     9181 ggccgccaat ccgaacgagg tcacgagggt ctggagttcc gacagttgcc tgttccaatg
     9241 gtctcgtttt cgcgcctcct gttcgactac accgaggccg caatccaggt cgtagcagac
     9301 cggttccaac acatgcatca acgtgactgt ggcgccgagg ccgttggcga gttgaatggc
     9361 atattcgacg gcatccagcg agggactcga aaaatccagc ggtgccagaa tatgccggat
     9421 gtgtacctgt ctgcctgccg cggacggcct gcccgcttct tcacgcaacc ctggtaccgc
     9481 gagaaccgga cagggccctt ctgtcacgac ccgttccgcc gtgctgccga tcagcccata
     9541 cagcaagttg gttcggccct ggacgcccag cacgacaagc tccgccccct tgtccttggc
     9601 agccagacca atgtgctctg ccgggttccc cagaaccagc cgcaccttga ctgagacgcc
     9661 acgccgcacc agatcgtcac gcacctgctc caactgccgc gcagcctccg ctcgcacctg
     9721 tacaaccgcg gcaagggatt ccgcctcggc gctcgaccgg tggggcgtct cgatcacatg
     9781 caggacctcc aacgtggctc cccagtggga ggcgagatag atggcatagt cctgcgcaca
     9841 ggccgcgcca tctgaaaaat cggtcgccaa gaggatccgt gtcatcagtg gcttagtcat
     9901 gatagctcct ctgaaacggt tgggcgactg ttcccatgga ggcgaccggt ctccggcaag
     9961 cggcgtcgga tggtcgatca aggagcggtg cgcgtgggtg atggcaatct gaacgcagac
    10021 ggtcaacggc tgatagcgcc ttcacgagca agacaaatgc cagcacctct cccgttcatc
    10081 cgttggtctc tcgcttattt tctgggcagg tataagacag agggccccga ttccaactcc
    10141 ccactgttgc catttgcagc agaggccctc cttgccatga ggcaatacac cccgccacga
    10201 cgagatcgca tcacctggtc gcactgtgcc ggtgcgtgcg attccgatct acacaacctg
    10261 cgcttcatcc tcttcctcac gatatccctg tcgtccggtt ctcaacctcc gacgtctcga
    10321 acggcgttct tgccatgttt cacgggcctc ctcagtaatc cgacctgttt ttcgtcgagg
    10381 aaggacagcc acagcattgt cccccacaca acacaggtga tccctcccac tgccaacagc
    10441 acaagcatgt cgtcacccct tctcgctcgg ccgctaaagg ccaagcgagc ccctaactag
    10501 ccaggactat tcatgaatgc cgtcgcgagg cgtttgagat cgaggcccgc ggggcgacag
    10561 gaccggacga cggagcaggc attcatgaac agtccgggat aggagtctgt ccgagtcatc
    10621 cttcgttgcg aggggaagga gctgctggga gatgcgagac cgcaggcacg aaaaatccgg
    10681 aggcgtattc gctggaatac gtcgaggatt gtttcgggcc aagaacgacg cagataccgg
    10741 cagctcgttt gccgcagcag aatggtatta ctcggacaga ctcctaagat ggattcagca
    10801 gagacaaacc ccagaagacc atcaccccca ttgcgccaac gaccgacaac agcagcagcg
    10861 tgaccggcac gatggtactc agtttgttca tctcgttcac ctttcatgat ccgcccgaga
    10921 cgggatcctc gtcgccccgc cccgtcacga tgtcactgta gccgcagaac atgaatcgac
    10981 gatgaacgga gcatgaagaa tttctccatg aatagtctgt catggaggct tcatccgcac
    11041 tgcatattgg cctgttacca agcgccggaa agtgagtcgt tggaagacgt gatacaacag
    11101 gcggtgacgg cctctgttgc tggtgccgct ctccattcca cgctgctgat gttcacggtg
    11161 atgcagaaac cggcaatcag gcagggccta ttacccccac gacaacgtgc tcgcacgcac
    11221 gacctgggtc agcccccaca gcagaaagag cccatagacc gtaccgaaga accaggggaa
    11281 gctgatcgcg cgatgcgccc accattggcg atgttgccgg aacgcccagg agggctcgaa
    11341 gaagcgcggc tcattcgtgt cctcgacgat cgtttcacga tgttcgcgga gtcgatagag
    11401 actgttcacc ggctttcgtt cccgccattc aggcatggtc tcgatgcccc gtagctgcca
    11461 ctcccagagc gcattttgcc cggagaaccg cccgagcacg atcgccatct gcacactcat
    11521 gagaatcccg atgatgctga acaatacaat gatgagggta aagggaagcg ccagcgattc
    11581 ccgcgacacc agcaacccga tcaccgcgac aaaaatcgaa ttggcggtca gatagtccga
    11641 cagcatcgtg tgatagtccc gcacgccgga cgtccacaac gtcaatagat gcgcataatc
    11701 ggatcgcaac acatcttgat ccagggccat cactttaggc tccattgacg tcttgtccga
    11761 ccagccgata cgcattgcgc agatactgcc ccaccatgcg ctgcgtgttg aagaacgccc
    11821 cgttcacggc aatcgcatgc ttcatgatct cgatgaatcc ctcccggtcc ttgtaaaagc
    11881 aaggcatgac ccgctgctca agcttgtcgt acagagcccg ggcatggcag gcctccatgt
    11941 cctccgcggg cgacacacag gcctcgatct tctcgccgat cgaccagccc gtgacccctt
    12001 cgatatgccc ctcgacccac caaccgtcca gcacgctcaa actcggaacc ccgttcatcg
    12061 ccgccttcat gccgctcgta ccggaggctt ccatcggcgg gagcggcgta ttcagccaca
    12121 catccacgcc ggcgcagagg agacgtgcca gcgtcatgtc gtaattggca agataggcca
    12181 ccgatagctg cccacgcatg ccgtaaatgc gatggatcat gtctttgccc gcctgatcct
    12241 gcgggtgcgc ctttcccgca aagacgaatt gaatcgggcc tgcctctttc aggagccgcc
    12301 gcaaccgttc gacatcgtga aacaccaggg tccctcgctt atacgcagtc gcgcggcggg
    12361 cgaagccgat ggtgaggaca tcgcgatcga atcccgcgtt ggtctgacgg ttcacttcgt
    12421 ccaccaaggc caacttggca tcctgatggg ccctccagat ttcagcgccg ggaatactga
    12481 tggcgtaacg aagcgataat tgatcgtagc gccagtcggg caaatgacgg tcatagaggt
    12541 gttgaaacga aggcgcggtc caggtcacgg catgcacgcc gttggtaatg gaatggatcg
    12601 gatagccggg atacaacgag tgggacacct cgccatgccg catggcgaca ccgttgacga
    12661 accgggatcc ccgcaaggcc agcaaggtca tgttcaacgc gccctggtgc tggcaggcct
    12721 gcacgagatc gcatcgccga ctccccaaca cccgatgcgc gagttccacc gggaattggt
    12781 cgtggcccgc cggcaccggg gtatgcgtgg tgaacacgca ttgctcccgc acct
//
LOCUS       DAOQRM010000071        12635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_4100_length_12635_cov_13.861208, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000071 DAOQRM010000000
VERSION     DAOQRM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4100_length_12635_cov_13.861208"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKK81_07335"
     CDS             <1..456
                     /locus_tag="PKK81_07335"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV32725.1"
                     /translation="QFAFTVPLTQPVIAALTPLLSDSDRSMATLARSALDKAKPDDAA
                     QLESLGMMVQHSAARDYALHQLAQLGPAAGDVLLPVLPLLQDDRPFVRYLAAEAIGAM
                     GPTAKQALQALQRRREDSDPVVREAVADAIAGIEAAMAPAASSEKATRP"
     gene            453..953
                     /locus_tag="PKK81_07340"
     CDS             453..953
                     /locus_tag="PKK81_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32726.1"
                     /translation="MIATTHETSSRLRLLTRLGFWGSVIGGLLFPWAIMLAVEVVVHQ
                     VPVARAWRSFTLHLFAPGYNFFLIGLLTAVPFVMLAVLMLLHLGAAPAQEPLIARRRT
                     LGLAGAGLGMLVLAGWTHLEVLLHPDAQGALAYLYLPVILLASMPIGYGLGRVIARML
                     LPRPSA"
     gene            1029..2006
                     /locus_tag="PKK81_07345"
     CDS             1029..2006
                     /locus_tag="PKK81_07345"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNV32727.1"
                     /translation="MIRLLWSLVLLILWHSAVLAASKYEDSVKELAEGVAAEAIKMKK
                     SRLALLDFVDSTGEVTPIGQFLAEELATHLLVAGELKVVDRKLLVATMEKHQLTHLDT
                     SQAKAAKKVAKALRADLYVTGAYLEIPEGVQVTAKLIGPYTVFPVGAARAIIPKAGPL
                     AALLKQANAPKPPAPATPPEPTTPVLNAHENELYKVSVMTISRQDDQIEIDLLFVNRS
                     THPVKIGCQLLDTYLVDEQGEQWRQDVAQNREGLCTRGMELNPRRQQHASLSFIATNK
                     PPTGPVTLHYHETSPRPDRVVTLDGLKLEAAPAAEAEAPAPQAADTPTP"
     gene            2043..2837
                     /gene="thiD"
                     /locus_tag="PKK81_07350"
     CDS             2043..2837
                     /gene="thiD"
                     /locus_tag="PKK81_07350"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNV32728.1"
                     /translation="MIKQVLTIAGSDSGGGAGIQADLKAMSANGVYAMSVITAITAQN
                     TEEVADVFELPTSIIAAQLDAIFDDFDVAAVKTGMLSSTDIVKTVVKLLKPQQVKNLV
                     VDPVMIAKGGQALLKPDAIDTIKKELFPLALLVTPNVHEAQQLSGIEITSLADARRAA
                     KIIHQFGCANVLIKGGHLASDRATDLLYDGRFFNIYKGEFIDTPHTHGTGCTFASAIA
                     AHIARGKTVPDAVQTAKTYLTEAIRHSLAIGHGKGPTNHFYFLNQG"
     gene            2842..4134
                     /locus_tag="PKK81_07355"
     CDS             2842..4134
                     /locus_tag="PKK81_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32729.1"
                     /translation="MSRGPGSPSSFLLTGWSWLLLTSLLGLATFLGIVRGSPLPPGLR
                     LLHVHGALIGGLLQIMTGLALTAVALAGHDTKGQKHRLLFVSMNLSALGLAAGAWLRD
                     STLTIGAGLLLAALFVPMVRHLMLGLRTSSGWTPLSTFYFGISLTGLFGTLALGEFLA
                     GAWFPTWHGILRLGHLHAGLLLFLTLGVVGAMQTTMPGLMNRPLRSAVLGQIVLLLLP
                     ACAAGLITGFLLSSVHIQLVAGGGFLVTLALGSVNLLRTWNQAGQPGSAATDHLMIAL
                     CLLLIMTCIGMAVGINVLWTPPVMPYGTLHLVAYTHTAFLGFFLQATVAGLSYALPAL
                     LAAQRVTSHKKRVAYQDTLAQIANRWRALQVSTLSFGTLGLVLVASLTWNLPLSSIWI
                     QAGTWGSLGLLLLHLTMVTMKITQMVGTMPTGDRAATH"
     gene            4155..>4719
                     /locus_tag="PKK81_07360"
     CDS             4155..>4719
                     /locus_tag="PKK81_07360"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV32730.1"
                     /translation="MPTIQPREETVFFHDRLGHRIAAVLARPEQTTDHVAVLCHGFLS
                     HKNSSSNQALTELLIGRGIATFRFDCFGHGDSDGPFAKLTTTIGVGQALAALHYLLTR
                     GYHRLGLVGSSFGGLLSILAAADWTRMHTSKPASIPPLACLALKCPVVDFGEELRLEL
                     GEDGLQEWKQTDTIPDLHGGATRLPLDY"
     assembly_gap    4720..4819
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5008..>5941
                     /locus_tag="PKK81_07365"
     CDS             5008..>5941
                     /locus_tag="PKK81_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32731.1"
                     /translation="MKTSTSVLVMGAVGCLSSALLQTGGASYAVAADSATTMSLLQQP
                     SLAAAVTKAQGSGTAPILPNPTALVLTAQKGNTAVGTLTLKKSSTDQHTYYLSTNQSW
                     IWMNPPYGSTQSITSETDQLVITAQTANLPAGTHSAVVYVVDSGPNNFTNMLRIPVTL
                     TVTASPVATPPPPPPAAPPAVTPPTPKPVVQTPPPPPPAPVAVPPPPAPAPPAPPLVS
                     GTGMVATPVALTLTAAKGQPAVGTLALRKGGTAQHTYSLSTNQSWIWMNPPYGSTQSI
                     ATETDQLVITAQTAALPAGTHSAVIYIVESGPNNF"
     assembly_gap    5942..6041
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6042..6278
                     /locus_tag="PKK81_07370"
     CDS             <6042..6278
                     /locus_tag="PKK81_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNV32732.1"
                     /translation="SATVSWNANTESDLAGYRIYVGTRSGSYGFAGPFEVTNRTNFTI
                     QNLPVGATYFFSVSAFDKSGNESAKSAEVSKSLF"
     gene            complement(6338..7174)
                     /locus_tag="PKK81_07375"
     CDS             complement(6338..7174)
                     /locus_tag="PKK81_07375"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNV32733.1"
                     /translation="MRLLVSLLILSLSITGLATGDSAYAQLERLRKNKEVEAAPLPDP
                     PMVSIPEGDFLMGADGTDALEDERPQHRVWVDRFEIDRYEVTTRRYAEFLSVTTRAEP
                     WQWEAVDPARHHDRPVIGVSWFDAEAYCRWRSARLPTEAEWEKAARGSDGQLFPWGNR
                     TPTDEVANFGLGARFSYSQVLAPVQGYQPGRSPYGLHQMAGNVGEWVADWYGANYYET
                     SASKNPAGPERGAFRVVRGGSWSDLPKYLLTYGRSKLPPETRNSYTGFRCARAVLPIT
                     SP"
     assembly_gap    7409..7508
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7509..7772)
                     /locus_tag="PKK81_07380"
     CDS             complement(<7509..7772)
                     /locus_tag="PKK81_07380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV32734.1"
                     /translation="MKRVLEPELMDDAAQARAYAKADFAEENQGFVDRFREYFPDWAG
                     GHVVDLGCGPGDIPIRFLRSFPDARVTAVDASRPMLDLAAEAIA"
     gene            complement(7769..8272)
                     /locus_tag="PKK81_07385"
     CDS             complement(7769..8272)
                     /locus_tag="PKK81_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNV32735.1"
                     /translation="MAQPVIPQVHQVSLSDGGVPKSAVLSAQVTCDGLVGDRQRNRKY
                     HGGPDRAVCLFSLEVIDALRAEGHSIGPGSSGENLTLAGLDWSLIRPGDRLRIGEQVR
                     LEMTSYTTPCRYNAQWFKDGDYERIAQETHPGWSRLYARVLHEGTVRPGDPVVLESDD
                     LSGWGDR"
     gene            complement(8280..9215)
                     /locus_tag="PKK81_07390"
     CDS             complement(8280..9215)
                     /locus_tag="PKK81_07390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNV32736.1"
                     /translation="MASTSKTDDCTADVSDTPGPGDWIQVDVQTSLDAGELLGVLNDP
                     DVTGAWQEQDCIHLYWPATRCGPDTWQGLRLALTRLGHPDPAGAITINRLADRDWNAQ
                     WSRLVEPIRVGRVVIRPSWKAVALNPDDIELLIDPKQAFGTGHHATTQLLIEWLQEVV
                     TPTDRVLDVGTGSGVLAMVALRLGASEAVGEDFDPVAIECARDYAQVNGFDARLTLAV
                     EYAQPQQSHHGFEPTLVLANLDRQTLLNAAETLCGYASGGARLLLSGLLIDQRAEIEA
                     AYAGHGVYVRASRERDGWVALEATGMESCDGGLCS"
     gene            9311..>10689
                     /locus_tag="PKK81_07395"
     CDS             9311..>10689
                     /locus_tag="PKK81_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32737.1"
                     /translation="MLRRAAQLKTNARRASARFTTWRLAIFAVGLFGSIIPHKLGWTL
                     LGNATLAFSFMLFLIVAWHHNRLEDRLHRLRRWQGIKQVHLARMRLGWADIPFRPVPV
                     PERHGYATDLDLAGPHSLLHLINNTVSSQGQERLASWLFDQPPEPAQWAVRQALIREL
                     TPLSLLRDRLSLEAQAVEDADIDGRRLLAVLQKRVDSPSFMPMLLTETFLALATAGLM
                     LTDLVYGIGNYWMLSFGLYAFLFLSVRSRSEEIFDHAQSLHRQLERLSAVIGYLERRS
                     YRAAPRLAELCRPLLQQDTSPSTSLKQAASVMNRLSVRAHPLVHYLINAFGPWDLYHT
                     YRLQHLQDRIAAVAPQWFELLAELDAAASLATFAYLHPDYPWPSLQQPDDAAHLNGAG
                     PILDAQGLAHPLIPAKARVANHIVFQERGRMFLVTGSNMSGKSTFLRTIGINTCLAQA
                     GGPVCAASFR"
     assembly_gap    10690..10789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10969..>12635
                     /locus_tag="PKK81_07400"
     CDS             10969..>12635
                     /locus_tag="PKK81_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV32738.1"
                     /translation="MRGLLIAQFCGAFNDNAWKFMVALLGIRAATAALAPGQDLETAS
                     QTQTTLAMVVFTLPLVLTSFVAGFFADRISKRTVIITMKAVEVLLMAAATLALLANPT
                     GGLWALVVLACMGVHSAIFSPAKYGILPELLPHEQLARGNSILELFTFLAILTGTTAG
                     GFLLAGAGTQPWLAPFALTLLALGGFAAAWAVPPVAPARDAGGLFDTLRGALSALQAD
                     RLLRLAITGAVFFWTIASLVVQNVLVYAKAVLGLSDALATVPSALISVGIGLGALVVG
                     RLSRSRVEYGLVPLGAAGVATFLLILGWWTPPFSGTLALMIALGMSSALIFIPINALV
                     QWRAPHDRRGSVIALENICVFTGILLGSLSGGILANVGLSTVGIFLAAALVTTIGTAW
                     AMWLMPETFLRLVLVLLTNTIYRLRIVGQQQVPVTGGALLVPNHISFIDGFLLIASLD
                     RPIRFVVDAQYVNHRLFKPFMRALQVIPISSAGGPRVILRALREAGHALDAGDLVCIF
                     PEGQITRTGNLLPFRRGFERIVKGRAVPVLPVHLDRVWGSIFSFVGGR"
BASE COUNT         2290 a         4185 c         3443 g         2317 t
ORIGIN      
        1 caatttgcct ttacggttcc cctgacacag cccgtgatcg ctgcgctgac accgctgctg
       61 tccgattcgg acagatcgat ggccacgctg gcccgcagtg cgctggataa ggcaaagccg
      121 gacgatgcag cacagctcga atcattgggc atgatggtcc aacattcagc cgcgcgcgac
      181 tatgccttgc atcagctggc ccagctcggt ccggcggcag gcgatgtcct actcccggtc
      241 ctgccgctgc tccaggatga ccggccgttc gttcggtacc tcgcagcaga agcaatcggc
      301 gcgatggggc ccaccgcgaa gcaagcgctc caggcccttc agcggcggcg agaagactcc
      361 gatcccgtcg tacgggaagc cgttgccgac gccatcgccg ggattgaggc cgcaatggca
      421 ccggcggcat cctccgaaaa ggcgactcgt ccatgatcgc gacgacacac gaaacctcct
      481 cgagacttcg cctcctgact cgcctgggtt tctggggaag tgtgatcggc gggctgctgt
      541 tcccctgggc catcatgctg gcggtggagg tcgtagtgca ccaggtcccc gttgcacggg
      601 cctggcgatc gtttaccctg cacctcttcg ctcccggcta taatttcttc cttatcggcc
      661 tgttgacggc cgttcccttc gtgatgctcg cggtgctgat gctgctgcac ctgggagccg
      721 ctcctgctca ggagccgctg attgcgcgtc gacggacatt ggggctcgcc ggcgcaggac
      781 tcggcatgct ggtccttgcg ggctggaccc atcttgaggt gttgctccat ccggatgcgc
      841 agggggcgct tgcctatctc tatctgccgg tcatcctgct ggccagtatg ccgatcgggt
      901 acgggttggg acgcgtcatc gccagaatgc tcctgccgag gccttccgct tgaatgacac
      961 gggctggaca gtctcgacga cgtgagtata atgtgggcca tgcgtgatta tgccgtgagt
     1021 gcctcccgat gatccgtctc ctctggtccc tcgttctgct catcctctgg cactccgccg
     1081 tcttggctgc gtcgaagtat gaagacagcg tgaaggaatt ggccgaaggg gtggcggccg
     1141 aagccattaa aatgaaaaag tcccggctcg cgctgttgga tttcgtggac agcacgggcg
     1201 aggtgacgcc catcggtcaa ttcctcgccg aggaactggc gacccatctg ctggtggccg
     1261 gagagctgaa agtcgtcgat cgcaagctcc tggtcgccac catggagaaa caccagctca
     1321 cccatctcga cacgtcgcag gccaaagccg ccaagaaggt cgcgaaggca ttacgcgccg
     1381 atctctacgt caccggcgcc tatcttgaaa ttcccgaagg cgtgcaagtc accgccaaat
     1441 tgatcggccc ctacaccgtg tttccggtgg gcgccgcccg ggcgatcata cccaaagccg
     1501 gcccgctcgc cgcgctgctc aagcaggcga atgcccctaa accgcccgca ccggcgaccc
     1561 caccggaacc gaccaccccg gtcttgaacg cccatgagaa tgagttgtac aaagtcagtg
     1621 tgatgaccat ctccaggcag gatgaccaga tcgagatcga cctgctgttc gtcaatcgca
     1681 gtacccatcc ggtcaagatc gggtgccaac ttctggacac gtacctggtg gatgagcagg
     1741 gcgaacagtg gcggcaggat gtcgcccaga atcgcgaagg cctctgtacg cgcggcatgg
     1801 aactgaaccc cagacggcag cagcatgcga gcctctcctt catcgcgacg aacaaaccgc
     1861 ccacgggacc cgtgacgctg cattaccacg agacctcgcc gaggcccgac cgtgtcgtca
     1921 ccctggatgg actgaaactc gaagccgcgc cggccgccga ggccgaagcg ccggccccgc
     1981 aggcagcaga cacccccaca ccataagtga ttatcttcgg aatagaggca caggaaacct
     2041 tcatgattaa acaagtcttg acgatagcgg gttcggactc gggcggcgga gcagggattc
     2101 aggcggacct gaaggccatg tcggccaacg gggtgtatgc gatgtcggtc atcaccgcca
     2161 tcacggccca gaacacggaa gaggtggccg acgtgtttga gttgcccacc tcgatcattg
     2221 ccgcccaact cgacgcgatc ttcgacgatt tcgacgtcgc ggcggtcaaa accggcatgc
     2281 tctcctccac cgacatcgtc aagaccgtcg tgaaactcct caaaccgcag caggtgaaga
     2341 acctggtggt agaccccgtg atgatcgcca agggcggtca ggccctcctg aagcccgacg
     2401 ccatcgacac gatcaagaag gaactgtttc cactcgccct gctggtgacg ccgaacgtgc
     2461 acgaagccca acagctctca ggcatcgaaa tcacctcgct cgccgatgcc cgccgggccg
     2521 ccaagatcat tcaccagttc ggctgcgcca acgtcctcat caaaggagga cacttagcca
     2581 gcgaccgcgc caccgacctg ctgtatgacg gccgcttctt caatatctac aagggtgagt
     2641 tcatcgatac gccgcatacc cacggcaccg gctgcacctt tgcgtcagcc atcgcggcgc
     2701 atatcgcccg ggggaaaacc gtccccgatg ccgttcaaac cgccaagacc tatctcaccg
     2761 aggccatccg acacagcctg gccatcggac acggcaaggg tcccaccaac catttctatt
     2821 tcttgaatca gggttagcgc tatgagtcga gggcccggat ccccatcgtc ctttctgctg
     2881 accggatgga gctggctgct gctgacgtca ctgctcggac tcgcgacctt tctcggcatc
     2941 gtgcgcggct ccccgctccc tcccgggctg cgcctattgc acgtccacgg ggcgcttatc
     3001 ggcggactcc tccagatcat gaccggcctg gcgctcaccg cagtcgccct cgccggccac
     3061 gataccaaag ggcagaaaca ccgcctgctg ttcgtcagta tgaatctctc cgccctcggg
     3121 ctggccgccg gggcctggtt gcgtgactcc accctgacca taggggccgg gctgttgctg
     3181 gccgcgctgt ttgttcccat ggtacgccac ctgatgctgg gacttcggac cagttccggc
     3241 tggactccct tgtcgacctt ctactttgga atcagcctca ccggactgtt cggcaccctg
     3301 gccctgggcg agttcctggc cggcgcctgg ttccccacct ggcatggcat cctgcgtctg
     3361 ggccatctgc acgcggggct tttgctcttc ctcacactgg gggtcgtcgg agcgatgcaa
     3421 acgacgatgc ccgggctgat gaatcgcccc ctgcgcagcg ccgtcctcgg acagatcgtc
     3481 ttgctccttc tgccggcttg cgccgcggga ctgatcacag gatttttatt gtcctccgtt
     3541 cacattcaac tggtcgccgg cggtggcttc ctggtgacgc tggcgctcgg cagcgtcaat
     3601 ctcctccgca cctggaacca ggccggccag ccgggatccg ccgccaccga ccaccttatg
     3661 atcgccctgt gtctgctgct catcatgacc tgtatcggca tggcggtcgg gatcaatgtc
     3721 ctgtggaccc cgccggtgat gccttacggg acgcttcacc tggtcgccta tacccacacg
     3781 gcgttcctgg gatttttcct acaagcgacc gtcgccggac tgtcttatgc gttgcctgcg
     3841 ctgctggcag cgcaacgggt caccagtcac aaaaagcggg tcgcctatca ggacaccctt
     3901 gcgcaaatcg cgaaccgatg gcgggcgctg caggtgtcca cgctcagctt tggaaccttg
     3961 gggcttgtcc tcgtcgcctc gctgacctgg aatctcccgc tttcttcgat ctggatccag
     4021 gcaggaacct ggggaagtct gggacttctc ttgctccacc tgacgatggt gacgatgaag
     4081 atcacgcaga tggtaggaac gatgccgacc ggcgaccgcg ccgcaacaca ttaaccagac
     4141 tcgaatcacc ggttatgcca acgattcagc ccagggaaga aacggtcttt ttccacgaca
     4201 ggctcggcca ccgtatcgca gccgtcctgg ccaggcctga acaaacgacg gatcacgtcg
     4261 cggtcctttg tcacgggttt ctctcccata aaaacagttc gagcaaccag gctctgaccg
     4321 agctcttaat cggccggggg attgccacgt tccggttcga ttgcttcggg catggcgaca
     4381 gcgatggacc cttcgccaaa ttgacgacca ccatcggagt cggccaggcg ctcgcagcct
     4441 tgcactacct cctgacccgg gggtaccacc gactcggact cgtgggctcc agcttcggtg
     4501 gtctcctgtc gattctcgcc gccgctgact ggacaagaat gcacacctcg aaaccggcat
     4561 ccattccgcc actggcatgc ctcgcgctga aatgtcctgt ggtcgatttc ggagaagagc
     4621 ttcgcctgga gctgggggaa gacggcctgc aagaatggaa acaaaccgac acgatacccg
     4681 atcttcacgg aggagccact cgccttcccc tcgactatgn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnc gcgtcccgcc ggaggctttc tcctgcagcc actgctccat
     4861 cttgcacagc aaaaaataac gactagaaac ccgcagctcg attgcccgca aaatggctcg
     4921 tgacggggct ttctggcgat cgcaccactt cgacgcctgg cacactggtt gctcatccag
     4981 cgtctcgatt ttcccggagg aactacgatg aagacgtcaa cgagcgtgct tgtgatgggt
     5041 gctgtcggtt gtttgtcttc cgccctgctc cagacaggag gggcttcata tgcggtagcc
     5101 gcggacagcg ccaccacgat gagtctgctg caacagccat cccttgcggc agctgtcaca
     5161 aaagcccagg gcagcggaac cgcacccatc ctgcccaacc cgaccgcgct ggtcctgacg
     5221 gctcagaaag gcaacactgc cgtcggtacg ctcacgctca agaagtccag cacggatcaa
     5281 cacacctact atctcagcac gaatcaatcg tggatctgga tgaacccgcc ctacggcagc
     5341 acccagtcca ttacctccga aaccgatcaa ttggtcatca cggcgcaaac cgccaacctg
     5401 ccggccggaa cacattcggc cgtggtctat gtcgtcgact cgggccccaa taacttcacc
     5461 aatatgctgc ggatcccggt cacgctcacg gtgacggctt cgccggttgc gacaccgccg
     5521 cccccgccgc ccgcagcgcc accggccgtg acccctccga cgcctaaacc cgtcgtgcag
     5581 acgccgcccc ccccaccacc cgcgcccgta gccgtgccgc ctccgccggc ccccgcgccc
     5641 ccggccccgc cgctggtctc cggaaccggg atggttgcca ctcccgtagc actcacgctg
     5701 acggcagcga agggacagcc ggccgtcggc accttggctc tccgcaaagg cggcaccgct
     5761 cagcacacct actccctcag cacgaaccag tcgtggatct ggatgaaccc gccctacggc
     5821 agcacccaat ccattgcgac agaaacagac caactcgtga tcacggcaca gaccgccgcc
     5881 ctgccggctg gaacacattc ggccgtgatc tacatcgtcg aatcaggccc caacaacttc
     5941 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtgccacg gtcagctgga
     6061 atgcgaatac cgagtcggat ctggcagggt atcggatcta cgtcgggacg cgatcgggca
     6121 gttatggatt tgcggggccc ttcgaagtca ccaaccgcac caacttcacg atccagaatc
     6181 taccggtcgg tgcgacctat ttcttttccg tatcggcctt cgacaagtct gggaacgaaa
     6241 gtgccaagtc tgcggaggtc agcaagagcc tgttctaagc gaacgcgaaa tagagaagag
     6301 cgagagaccg tgagctggcg cgcagcaggc gcccagctta cggggaagtg atcggaagga
     6361 ccgccctggc gcagcgaaat cccgtatagc tattgcgagt ctccggcggc aacttcgacc
     6421 gaccgtaggt cagcaggtac ttcggcaagt ccgaccagga cccgccacgc acgacacgaa
     6481 acgctccccg ttccggtccg gctggattct tcgaggcgct ggtttcgtag taattggccc
     6541 cgtaccaatc ggcaacccat tccccgacat tccctgccat ctgatgcagc ccataggggc
     6601 tgcgaccggg ctgatatccc tgaacgggcg cgaggacctg actgtaactg aatctggcgc
     6661 caagcccgaa attcgccacc tcatcagtcg gagtccgatt gccccagggg aacaattgtc
     6721 catcgctccc ccttgcggcc ttctcccact cggcttcagt cgggagccgt gcactccgcc
     6781 aacgacaata ggcctccgca tcgaaccaac taacgccgat gaccgggcga tcatgatgcc
     6841 gggccggatc caccgcctcc cattgccagg gctcagcccg ggtcgtcacc gagagaaatt
     6901 cggcatacct ccgggttgtc acctcgtacc ggtcgatctc gaaccggtcg acccataccc
     6961 ggtgttgcgg ccgctcatcc tccagcgcat ccgttccgtc ggcccccatg agaaagtctc
     7021 cttcaggaat cgagaccatg ggcgggtcgg gaagcggcgc agcttcaacc tctttgtttt
     7081 tccgcagccg ctcaagctgt gcgtacgctg aatcgccggt tgccaaaccg gtaatcgaga
     7141 gagacaggat gaggagactc acaagcaatc gcatgccggg aagcatacac aaacagcccc
     7201 ccaggcgcga gcgctctgcg agaaccctga acgagtcatg agaagagcag ggtggtcaaa
     7261 acgaggtgag aacgccgttg gagctcgttt tcaccatggc tgattaaaga aggattccac
     7321 cgacgaccca atgccggtca tccggcacgt cgatgaggag ccgggagagg ttcatctcgg
     7381 ccaattgcgc cgcgacatca tcctcggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnngg cgatcgcctc cgccgccaaa tccagcatgg gacggctggc atccacggcc
     7561 gtcactcggg cgtccgggaa agagcggaga aaacgaatgg gaatatctcc cggtccgcat
     7621 cccagatcga cgacatgacc gccggcccaa tcgggaaagt attcgcgaaa acgatcgacg
     7681 aaaccctgat tctcctcagc gaaatccgcc ttcgcatagg cacgggcctg cgccgcatcg
     7741 tccatcagct ccggttccag cacgcgcttc atcgatcacc ccacccgctt aggtcgtccg
     7801 attccagcac aacaggatct cccggccgca ccgtcccttc gtgaagaacc cgcgcataga
     7861 gccggctcca tccgggatgc gtctcctggg caattcgttc atagtccccg tccttgaacc
     7921 actgcgcatt gtatcgacag ggcgtcgtat aactcgtcat ctccagccgc acctgctcac
     7981 cgattcgaag tcgatcaccg ggccggatga gtgaccagtc gagccccgcc agagtgagat
     8041 tctccccgga cgaaccagga ccgatcgaat gaccttcagc ccgaagggcg tcgatcacct
     8101 ccagggaaaa gaggcagacc gcccgatccg gaccgccgtg atatttccgg ttgcgctgac
     8161 gatccccgac caatccgtca cacgtcacct gcgcagacag taccgccgac ttcggcaccc
     8221 cgccgtcaga cagactcacc tgatgcacct gtggaatgac cggttgtgcc atctcggtat
     8281 caggagcaga gcccgccgtc acaggattcc atgccggtgg cctccagggc gacccatccg
     8341 tcacgttctc tcgatgccct cacgtagact ccatgcccgg cataggcggc ttcgatctcc
     8401 gcccgctgat cgatcagcag accggacagc agcaggcgcg cgccgcccga cgcatatcca
     8461 cagagcgtct ccgcggcatt cagcagggtc tgccgatcca aattcgcgag gaccagagtg
     8521 ggctcgaatc cgtggtgaga ttgctgcggc tgagcatatt ccaccgccaa tgtcagccgc
     8581 gcatcgaacc cgttgacctg agcgtagtcc cgcgcgcact caatcgccac cggatcgaaa
     8641 tcctccccga ctgcctccga ggccccgagc cgcaacgcca ccatcgccaa cacgccgctc
     8701 ccggttccta catccagcac gcgatcggtg ggcgtcacca cctcttgcag ccattcgatc
     8761 aacagctggg tggtcgcatg gtggccggtg ccgaacgcct gcttgggatc gatgagcaat
     8821 tcgatgtcat cgggattcag cgccaccgct ttccaacttg gcctgatgac cacccgcccc
     8881 accctgatcg gctccaccag acgcgaccac tgggcgttcc agtcgcggtc cgccaggcgg
     8941 ttgatggtaa tcgcgccggc cgggtcaggg tgcccgagcc gggtcagtgc aagcctgagg
     9001 ccctgccagg tatccggtcc gcaacgggtc gcgggccagt agagatggat acagtcctgt
     9061 tcctgccagg ctcccgtcac atccggatcg ttcagcaccc ccaggagttc accggcatcg
     9121 agcgaggtct gcacatcgac ttgaatccag tcgcccggac cgggcgtgtc ggacacatca
     9181 gcggtacaat catctgtctt cgaggttgac gccatctcag actcccagta tcgtcagcgg
     9241 tacgatgaaa cctgatacag aatccacacc atcgcgaagg ttgcccacac gggaacacag
     9301 tctcgtacgc atgctgcgtc gcgcggcaca gctaaagaca aatgcccgtc gggccagcgc
     9361 aaggtttacg acctggcggc tcgcaatctt tgccgtcgga cttttcggct ctatcattcc
     9421 gcataaactg ggatggaccc tgctcggcaa cgccaccctc gcgttcagct tcatgctgtt
     9481 cctcattgtg gcttggcatc ataacaggct cgaagatcgc ttgcaccggc tccgccgctg
     9541 gcagggcatc aagcaggtgc acctcgcgcg catgcgtctg ggctgggccg acattccatt
     9601 tcgcccggta cccgtgcctg aacgacacgg gtacgcgacc gacctcgacc tcgccgggcc
     9661 ccattcactc ctgcacctca tcaacaacac cgtctcgtcg cagggccagg aacggctcgc
     9721 ttcgtggctg ttcgatcaac cgcccgaacc cgcgcagtgg gccgtccggc aggctctcat
     9781 cagggaattg acgccgcttt ccctgctcag ggaccgatta tcattggaag cgcaggcggt
     9841 tgaagacgcc gatatcgacg gccggcggct cctcgcagtg ctgcagaagc gggtcgacag
     9901 tcccagcttc atgcccatgc tactgaccga aaccttcctc gcccttgcca cggctgggct
     9961 catgctgacg gatctcgtgt acggaatcgg caactattgg atgttgtcgt tcggtctcta
    10021 tgcgttcctg tttctctccg tccgcagccg ctcggaagaa atcttcgacc atgcccaatc
    10081 actgcaccgg caactggagc ggctcagcgc cgtcataggt tatctggaac gacgatcata
    10141 ccgtgccgcc ccccggttgg cggaactgtg tcggccgctc ctgcaacagg acaccagccc
    10201 ctccacctcg ctgaaacagg ccgcctccgt gatgaaccgg ctcagcgttc gggcccatcc
    10261 gctggtgcat tacctcatca acgccttcgg gccctgggac ctctaccaca cctatcgcct
    10321 ccagcaccta caagatagga ttgccgccgt cgcgccgcaa tggttcgagc tgctggccga
    10381 attggacgcc gccgcctctc tggcgacgtt cgcctacctg cacccggact acccctggcc
    10441 ctcgctgcag cagccggatg acgccgccca tctcaacggt gccggaccga tcctcgatgc
    10501 gcagggactg gcgcacccgc tcatccccgc gaaggcccgc gtggccaatc acattgtgtt
    10561 tcaggaacgc gggcgcatgt tcctggtgac cggctccaac atgtccggca agagcacctt
    10621 tctccgcacc atcggcatca acacctgcct tgcgcaggcc gggggcccgg tctgtgccgc
    10681 ctcgttccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tcatggcact
    10801 cgaagggctg ccggtggaaa gtccaccctc gacgacatca cgatagcgca ttgcatcagt
    10861 tcaataattt cggcagactg atcctcgatt ccggacacga acgacggcac atagacacag
    10921 gaggcaccca tcgaccccca gcccgactcc tcatcgaccc gacttccctt gcgcggactc
    10981 ctgatcgcac agttctgcgg cgccttcaac gacaacgcct ggaaattcat ggtcgcgctg
    11041 ctgggtatcc gggcagccac agccgcactc gcgccgggcc aggatctgga aaccgcatct
    11101 cagacccaga cgaccctcgc catggtcgtg ttcaccctgc cgctggtcct gacgtcattc
    11161 gtggccggat tctttgccga ccgtatcagc aagcgcaccg tcatcatcac gatgaaggcg
    11221 gtggaggtgc tgctcatggc cgccgcaacc ctcgcgctcc tggcaaaccc gaccggaggg
    11281 ctctgggcac tcgtcgtgct cgcctgcatg ggcgtccaca gcgcgatctt cagtccggcc
    11341 aaatacggca ttcttcccga actgctcccg cacgaacaac tcgcgcgagg caacagcatc
    11401 ctggagctgt tcaccttcct ggcgatcctc accggcacca ccgccggagg tttcttactg
    11461 gccggggcag gaacacaacc ctggctggcc cccttcgcgt tgaccctgct ggccctgggc
    11521 ggattcgcgg cagcctgggc cgtaccgccg gtggcgccgg cacgcgatgc gggcggattg
    11581 ttcgacacct tgcgcggagc cttgtcggca ttgcaggcgg atcggctgct gcgtctcgcc
    11641 atcaccggcg cggtcttctt ctggaccatc gccagcctcg tcgtccagaa cgtattggtg
    11701 tatgcgaaag cggtgctggg cctctcggat gccttggcga ccgtcccctc cgccttgatc
    11761 tcggtgggta tcggactggg cgcgctcgtc gtcggccgcc tctcccgttc ccgcgtggaa
    11821 tacggactgg tgcccctggg agcggccggc gtcgccactt tcctcctgat tctcggctgg
    11881 tggacgcccc cgttcagcgg cacgctggcg ctcatgatcg cgctaggcat gtcgagtgcg
    11941 ctcatcttca tccccatcaa tgccctggtg cagtggcgcg cgcctcacga ccggcgggga
    12001 tcggtcatcg ccctcgaaaa catctgtgtc tttaccggta tcctgctggg gtcgttgagt
    12061 ggcggtatcc tggccaacgt aggcctttcg accgtcggca ttttcctcgc ggctgccctc
    12121 gtcacgacca ttggaacagc ctgggccatg tggctcatgc cggaaacctt tctgcgattg
    12181 gttctcgtcc tgctgaccaa taccatctac cggttgcgca tcgtcggaca gcagcaggtg
    12241 ccggtgacgg gcggcgccct tctggtcccc aatcacatct ccttcatcga cggctttctg
    12301 ctgatcgcga gcctggatcg tcccatccgg ttcgttgtgg atgcccagta cgtcaatcac
    12361 cggctcttca agccgttcat gcgggcgttg caggtcatcc cgatttcatc ggctggcggt
    12421 ccccgcgtga ttctccgcgc cctgcgcgag gcgggtcatg cgcttgatgc cggtgacctt
    12481 gtctgcattt tccctgaagg ccaaattacc cgcaccggaa acctcctccc gtttcgccgc
    12541 ggattcgaac ggatcgtcaa aggacgggcg gttccggtcc taccggttca cctggatcgc
    12601 gtctggggca gcattttcag ttttgtcggg gggcg
//
LOCUS       DAOQRM010000072        12632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_4102_length_12632_cov_19.303650, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000072 DAOQRM010000000
VERSION     DAOQRM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12632
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4102_length_12632_cov_19.303650"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..1618)
                     /locus_tag="PKK81_07405"
     CDS             complement(365..1618)
                     /locus_tag="PKK81_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV32739.1"
                     /translation="MYRKIVLDNRLRIVAELLPTLKSVTIGIWVNVGSRDEQPGEEGL
                     SHFLEHMFFKGTRSRTATQISREIDALGGEMNAFTTRETTTFYVKVLDQQLEAALELL
                     SDLFYRSRFESKEVEKEKQVVLEEIRMVQDDPEDLVQELHMKHTLGSHPLGRPILGQA
                     PRIQALGRNDLVSYVGSHYDPERTVVAVAGNFTWRRLEQLLARYFSDSHKGVAARPSR
                     RPPEVKGGVLVKRKALEQVHLCLGLQGLSAGHKDRYAAHALNGVLGGSVSSRLFQEVR
                     EKRGLVYSIYSFLSTYSDGGMTTVYAGTRPKEVERVVEVVCRELKKLRTHGIDAKDLA
                     RVKNQMKGSLMLSLESSHSRMSKLAKDELTQGNHVSLEQMIAEIDRVTTDQVYRVAQT
                     LLDQRCLSITALGPIPTKSLQSFAS"
     gene            complement(1625..3742)
                     /gene="pnp"
                     /locus_tag="PKK81_07410"
     CDS             complement(1625..3742)
                     /gene="pnp"
                     /locus_tag="PKK81_07410"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNV32740.1"
                     /translation="MKHVVDIELAGRRLTLETGRIAKQADGAIWATYGDTVVLATAVA
                     SQNAKPGVDFLPLTVDYQERTYAAGKIPGGYFKREGRPSEREVLTSRLIDRPLRPLFP
                     EGYYFETQVIASVLSADKTGVSDVIGIIAASAALAVSSIPFNGPIAGVKIGRVNGQLV
                     VNPDLETLETSDLHLVVAGTADAVMMVEAGANELPEATMLEAIELAHSEIKKIVAKIE
                     ELRKLAGKPKRTVVQEPIDAALTEQVRALVAGPIREAILIPNKSARQERLDQVLAETV
                     AKLKSDEPNRDRHVKIIFHGLEYTEVRNMILEKRVRADGRGPADIRPITCEVGVLPRA
                     HGSAVFTRGETQSLAVVTLGTTDDEQRIDALEGEYMRTFMLHYNFPPFSVGEARPLRS
                     PGRREVGHGALAERALKSILPGKDKFPYTVRIVSEILESNGSSSMATVCGGTLALLDA
                     GVPIKEPVAGIAMGLIKEGNQVLVLSDILGLEDHLGDMDFKVTGTKNGVTALQMDIKI
                     GGITSELMREALAQAKAGRLHILGCMAQALTEPRTKLSAFAPRIFPMKIKQDKIRDVI
                     GPGGKMIRSIIAETGVKINVEDTGDVTIASSDEASAQKAIDMIKRLTEEVEVGKIYLG
                     TVRKIMDFGAFVEVLPGTDGLVHISQLAHHRVKAVTDEVSEGDQVMVKVLEIDKQGKI
                     RLSRKEAMPAPAGSPATEPTPAG"
     gene            complement(3939..4208)
                     /gene="rpsO"
                     /locus_tag="PKK81_07415"
     CDS             complement(3939..4208)
                     /gene="rpsO"
                     /locus_tag="PKK81_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNV32741.1"
                     /translation="MALVKEVKTELVKGFQQHDKDTGSPEVQIAILTNRITYLTEHFK
                     LHKKDHHSRRGLLTLVGRRRRLLDYLRKVEEARYRAILERLGIRK"
     gene            complement(4215..5246)
                     /gene="truB"
                     /locus_tag="PKK81_07420"
     CDS             complement(4215..5246)
                     /gene="truB"
                     /locus_tag="PKK81_07420"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNV32742.1"
                     /translation="MTMIASMEPRATTVLQDGVLNVRKEAGWTSHDVVARIRGKLRGM
                     KLGHAGTLDPAATGVLPLLVGRGTRIAEYLLEWDKTYLAGLRLGETTDTQDATGTVLL
                     RSPIESLSEARIREVVAGFEGRIQQLPPMYSAVKVGGVPLYKAARAGREVSRQVREVT
                     VFRLDIVDVQIPEVTLRVVCSKGTYIRTLCADIGDKLGVGGHMATLVRERVGPLTLEQ
                     ALTVDEVESRLEQGTLAASMLTLDQALVGLPVCIVGAATAGRVLHGMPVPASEVLAWH
                     GLSSPLSEGANRAIGIKDGAGRLLAIGTMPDGMNAEAPGANAQSIAVSKVLVTEESQG
                     GSIANSIEE"
     gene            complement(5250..5648)
                     /gene="rbfA"
                     /locus_tag="PKK81_07425"
     CDS             complement(5250..5648)
                     /gene="rbfA"
                     /locus_tag="PKK81_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNV32743.1"
                     /translation="MTKSTYSRADRVADQIRMEVADILMRKIKDPRVRSVTVTDVELT
                     KDLRIARVFVTTMEQNKDERLVFDGLAKASGFVRAELGRRLALRYLPELIFMKDISGP
                     RADRVLELLDGLHRDQSHEEMLAEPPHTDV"
     gene            complement(5658..5939)
                     /locus_tag="PKK81_07430"
     CDS             complement(5658..5939)
                     /locus_tag="PKK81_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNV32744.1"
                     /translation="MTVGLCTVELFIPDGHSLKEKRQVLQSLKTRLRDKFNLSVAEVG
                     DQDLWQKAILGMACVANESAHVNQVLDQAVNLIQSVPVIQLVRSRIELL"
     gene            complement(5936..8533)
                     /gene="infB"
                     /locus_tag="PKK81_07435"
     CDS             complement(5936..8533)
                     /gene="infB"
                     /locus_tag="PKK81_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNV32745.1"
                     /translation="MRVYELAKQLGMENRELIPELKRLGIPVASHSSALDDDSVRVVL
                     EKLSSKARGGEAVSGGHEAKKMVRSSKEPGASHERGHAAAHEEPPKPDKKRILIKKKR
                     EEGAEDVAASLAAAEAAFAPVVPAGTEASVVAPAAGPDAEAIEAAPVEPAPPEELVSP
                     PPVVTVSEEPLVKPVQVPQVTAPPLDALATAKKKSAATEAMESEAAAREKLKKAKKAP
                     RTREEDEAKFKNDATRWGDLRAIPVQRREDRSKHIHHASPTEVTKPRKKSVKLSAGVS
                     VKEFAELIGQRPADVVRKLMEMGQMVTFNQSINLEAASLIAEEYGTRVEVSTEKVGEA
                     LLEEAAQSSGEEHAVLRPPVVTIMGHVDHGKTSLLDAIRLTKVAEGEAGGITQHIGAY
                     IVGVRGKQVTFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTVEAIHHAKAA
                     GVPLIVAINKVDKPGANIDRVKNALTEHGLVPEPWGGDTIMVEVSAKQRTGLDQLLEM
                     ILLQAEVLELKADPSRMAKGLVIEAKLDRGRGPIATVLVQSGTLHVGDAFVVGNFSGR
                     VRALVTDTGSKTTEAGPSVPVEVIGLPGVPSAGDLFTIVKDERVAREIAQERAMKQRA
                     ADLAGPAKVSLDDLFAKIQEGNVKELPIVIKADVQGSAEALAAAVEKMPAGAVKLRVM
                     HTGVGGITETDILLAAASKAIVIGFNIRPEPKAAALAEREGVDVRLYSIIYDALNDIR
                     AAMEGLLEPTLKERVLGRAEVRQMFTIPKAGLVAGCYVVDGVISRASVGVRVIRDSVV
                     AYEGKLGSLRRFKDDVREVQQGYECGITVENFNDLKAGDIIEAYVVDKIAAKLEPANR
                     GTSPQSHRA"
     gene            complement(8597..9736)
                     /gene="nusA"
                     /locus_tag="PKK81_07440"
     CDS             complement(8597..9736)
                     /gene="nusA"
                     /locus_tag="PKK81_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNV32746.1"
                     /translation="MNRELIAVIDEIGRQKGIDKARVIGAIESALQTAAKKRFGQAEN
                     IQVEIDPKTGEISVVSKKIIVDTVANPKAEISLKEAREYDEGAEVGDEIGSLIEMDEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVNGIILGMERRNFLVDLGKTEAI
                     LPIQEQIPRETYRRGDRVKALLLEVRRTPKDVQVILSRSHPQFVAKMFELEVPEVGEK
                     IVEIKSIVREPGDRTKIAVSSRDKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWT
                     QDPRVFIAEALNPATIEKVGIDEEKKSALVVVADSQLSLAIGKNGQNVRLAARLTGWK
                     IDIISATEYEKEKAERDRDIKAALAEETEAQRQQDEARAAAQQAE"
     gene            complement(9779..10297)
                     /gene="rimP"
                     /locus_tag="PKK81_07445"
     CDS             complement(9779..10297)
                     /gene="rimP"
                     /locus_tag="PKK81_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNV32747.1"
                     /translation="MSDAGAVSAGKPSVKRAEALNLRVQEIAAPILQSYGLELVEAVC
                     VGQGPRTVIRVFIDKPGGITLTDCEQAHRSLSPALDVIDPFPHAYTLEISSPGLDRPL
                     RSVQDYRRLIGKPVTLKLCEPIQAQWRLVGAVVEVDDRGVTLAIQQKKTTETIRVEFD
                     QIALARRKVEFS"
     gene            complement(10388..11227)
                     /gene="dat"
                     /locus_tag="PKK81_07450"
     CDS             complement(10388..11227)
                     /gene="dat"
                     /locus_tag="PKK81_07450"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HNV32748.1"
                     /translation="MPDIAYVNGRFGPLADAVVSIEDRGFQFGDGVYEVIRTYRGQPF
                     AIEEHLVRLERSAQALQLAIGQTRAQWSSLIREGLRLSQFPEAKIYLQITRGQAPRDH
                     PFPVSSAPTTVLTFRELHPLDVSVRNAGVQAMSVEDIRWGRCDIKSVNLLANVLARQR
                     AKEAGVFEAILVREGTVTEGSVSNVMVVRNGAVQTAPEGRRILSGVTRAIVLDLARKE
                     GIPVAESFVTREELLAASEVFLTGTTVEVLPVVRVDGHMIGGGSPGPVSQLLSRRFEA
                     LVG"
     gene            complement(11255..11587)
                     /locus_tag="PKK81_07455"
     CDS             complement(11255..11587)
                     /locus_tag="PKK81_07455"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNV32749.1"
                     /translation="MSPLSKAVPTGAEQEPGAKTLKARVGASVHIRLWEDRTRGELWV
                     PSYDATVMPLVEDDFLRTASNNAVDAGQRTFEFRPVAAGTHRLVFEKRMGWKFTAEDR
                     QIFYVTVS"
     gene            complement(11626..11943)
                     /locus_tag="PKK81_07460"
     CDS             complement(11626..11943)
                     /locus_tag="PKK81_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNV32750.1"
                     /translation="MEGFQCVAKVEEIPPGQVKVVKVNERGIAVFNIDGQFHAIYNSC
                     PHEGGPLNKGRVKGHVVSCPWHDLAFDVRNGQGTDGGGYCVGSYDVRVEDGQVFVGGR
                     RKV"
     gene            complement(11960..12457)
                     /locus_tag="PKK81_07465"
     CDS             complement(11960..12457)
                     /locus_tag="PKK81_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNV32751.1"
                     /translation="MVRRASAAESEQRRKKIVTFVLLVILLVSVSLFLGGPKESELII
                     VEFPNGKTMETEVASTPEKLLFGLAFREGLPADTGMLYIFESTGLHRVQTRQFRIAVD
                     MVWIDESHHVVHILEQVPPCEKDPCPLYGPPPEPVRYLIEAEAGSVQRAGITTGMELK
                     FTLRM"
BASE COUNT         2633 a         4193 c         3157 g         2649 t
ORIGIN      
        1 tccttggaaa aatgatgacc agagaaccgt tgaatcacag tatccaaaaa aagagctcag
       61 aaaaaaacaa aattccagag aacgcgctag gaggaggaag tgcgacgcac accccctttc
      121 tctctgctta ccttcccctg taatgcagcc gaaataaatt cagacttata tgatgaaaac
      181 gcggccatgg tacttaaatc cacgaaacgt gtcaagaaaa tgttttgccg aaatccagcc
      241 atgattcgcg ccctaaaatg agtaacgtag caaagagtta gtcctgcaat ggcgctcaaa
      301 tcaccgctac tcattacccg accagccctt gcgccccgcc tacgggtgcc ccgcaattga
      361 ccggttatga cgcaaacgac tgcagactct tggtcggaat cgggccaagt gccgtaattg
      421 aaagacagcg ctgatccagt aacgtttgcg ccacccgata cacctgatcg gtcgtcacgc
      481 gatcgatttc cgcaatcatc tgctcgagcg acacgtgatt gccttgcgtc agttcgtcct
      541 tcgccaactt gctcatgcga ctgtgcgaac tctccaggct gagcatcaga ctgcccttca
      601 tttggttttt cacccgggcc aaatccttcg catcgattcc atgcgtgcgg agcttcttca
      661 gctcccggca gacaacttcc acgacacgct caacctcttt cgggcgcgta ccggcataca
      721 ccgtggtcat tccgccatcg gagtaagtgg acagaaacga atagatcgag tacacgaggc
      781 cacgcttttc ccgtacctcc tgaaacaggc gtgagcttac actcccgcct agcaccccgt
      841 tgagcgcatg cgccgcatat cgatccttat gcccggcact cagaccctga aggcccaaac
      901 aaagatggac ctgttcaaga gccttacgtt tgaccagcac cccacccttc acttcaggcg
      961 ggcgacggct tggccgggcg gccacaccct tgtgcgagtc ggaaaaatac cgcgccaaca
     1021 actgctccag gcggcgccag gtgaagttgc cggccaccgc aacgacggtg cgctccggat
     1081 catagtgcga accaacgtag gacaccagat cgttacgccc aagagcctga attctcggag
     1141 cttgccccag gattggcctg cccagcggat ggcttccgag cgtgtgcttc atgtgaagct
     1201 cttgaaccag atcctctgga tcgtcttgga ccatccggat ctcttcgagc accacctgct
     1261 tttctttttc aacttctttc gactcgaacc gggaccgata gaacagatcc gagagcagct
     1321 ccaaagctgc ctccaactgt tgatccagca ccttcacata aaaggtcgtc gtttctcggg
     1381 tggtgaacgc attcatctca ccccccagcg catcgatctc acgggaaatc tgcgtcgccg
     1441 tgcggctgcg cgttcccttg aagaacatat gctccaggaa gtgagacagc ccctcttccc
     1501 ccggctgctc atcgcgcgat ccgacgttga cccatatgcc gatcgtgacg gacttcaacg
     1561 tcgggagcag ttcggccaca atgcgcaacc ggttgtcgag aacgatcttg cgatacacga
     1621 tcgattatcc cgcgggcgtt ggttcggtgg caggagaacc agccggtgcc ggcatggctt
     1681 ccttccggct taagcgaatc ttcccctgct tatcgatttc cagcaccttc accatcacct
     1741 gatcgccttc ggacacctcg tcggtgaccg ccttgacgcg atgatgagcc agctgggaga
     1801 tatgcaccaa tccgtccgtg ccgggaagca cttcgacaaa tgcgccgaaa tccataatct
     1861 tccggacggt tccaagatag atcttgccga cctcgacttc ctcggtcagg cgcttgatca
     1921 tatcgatggc cttctgtgcc gatgcttcat cggaagaggc aatcgtcaca tcgccggtgt
     1981 cttcgacatt gatcttcaca ccggtctccg cgatgatgct gcggatcatc ttcccaccag
     2041 ggccgataac atcgcggatc ttgtcttgct tgatcttcat cgggaatatg cgcggagcga
     2101 aggctgacag cttggtgcgc ggttcggtca acgcctgggc catacacccg agaatgtgga
     2161 gccttcccgc cttcgcctga gccagcgctt cgcgcatcaa ttcggaggtg atgccgccga
     2221 tcttgatatc catctgaagc gccgtcacgc cgtttttcgt ccccgtgacc ttaaagtcca
     2281 tatcgcccag gtgatcctcc aatccgagaa tatcggacag aaccagcacc tgattgcctt
     2341 ccttgatcaa ccccatcgcg atccccgcca ccggctcctt aatcggaacg cccgcatcca
     2401 agagcgccaa cgtaccgccg cagaccgtgg ccatggaaga ggatccgttc gactccagga
     2461 tttccgatac aattcgcacc gtgtaaggaa acttatcctt gccgggaaga atcgacttga
     2521 gcgctcgctc ggctagagct ccatgcccga cctcgcggcg gcccggcgac cggagcggcc
     2581 gtgcctcgcc gacactgaag ggcgggaaat tgtaatgcaa catgaaggtg cgcatgtatt
     2641 cgccctccaa ggcatcaatg cgctgctcgt cgtcggtcgt acccagcgtc accaccgcca
     2701 ggctctgcgt ttctccgcga gtgaagactg cggatccgtg cgcgcgaggc agcacaccga
     2761 cttcgcaggt gatcggacga atgtccgctg gaccacgccc gtcggcgcgc acccgctttt
     2821 cgagaatcat gttgcgaact tcggtgtact ccagaccatg gaaaatgatc ttgacatgcc
     2881 ggtcccgatt cggctcgtcc gatttgagct tcgcaaccgt ctcggccaac acctgatcca
     2941 ggcgctcctg ccttgcgctc ttattgggga tgaggatggc ctcgcggatc ggaccggcca
     3001 ccagcgcacg cacctgttcg gtcaatgcgg catcgatggg ctcttgcacc accgtccgtt
     3061 tcggcttgcc ggccaacttg cgcaattctt cgatcttcgc gacgattttc ttgatttcgc
     3121 tgtgcgccaa ctcaatggct tccagcatgg tcgcttccgg caattcattg gccccggctt
     3181 cgaccatcat caccgcatcg gcagtcccag ccacgacgag atgaagatcg ctggtttcca
     3241 gggtttccag atcggggttc accaccaact gcccgttgac gcggccgatt ttcacgcctg
     3301 cgatcggccc gttgaatgga atcgacgaga ccgccaacgc cgcggatgcc gcgatgatgc
     3361 cgatgacatc cgacacgccc gtcttatcgg cagacaatac cgaggcaatg acttgcgtct
     3421 cgaagtaata cccttccgga aagagaggcc gaagcggccg atcgatcagc cggctggtca
     3481 gcacttcccg ctccgaaggc cgaccttccc gcttaaaata gccgcccgga atcttaccgg
     3541 ctgcataagt cctttcctgg taatccacgg tcagcggaag gaaatccact cccggcttgg
     3601 cattttgtga cgcaacggcc gttgcgagca cgacggtatc accgtaggtg gcccagatgg
     3661 cgccgtccgc ctgcttcgcg atacgaccgg tctccagcgt caaccgacgc cctgccagct
     3721 caatgtctac aacatgtttc atctatggtc tcctccgttt aaatcccaca gatgcccaca
     3781 gagataagtt caaccggcca tctacacatc caacgacaag cgcctgccct cgattgaatg
     3841 tcgacccgtg ccgttgaact tgtttcagtg ttcacctgcg gggacaacaa atggttcggc
     3901 cgccacccac gaaggtggca gccgattcgc tcacacgact acttacgaat acccagtcgc
     3961 tccagaatgg cgcgataccg ggcttcttca acttttcgga gatagtccaa caaccgacga
     4021 cgacgtccga ccagtgtcaa caacccgcgc cgggaatggt ggtccttctt gtgcaacttg
     4081 aaatgttccg tcaagtacgt aatccggttc gtcaggatcg cgatctgcac ttccggagat
     4141 ccggtgtcct tgtcatgctg ctggaaaccc ttgaccaatt ccgttttcac ttcttttacc
     4201 agtgccatac gtctctactc ctctattgag ttcgcgatac taccaccctg tgattcttct
     4261 gtgaccaata ctttcgatac agcaatcgac tgcgcattcg cccccggcgc ctcagcattc
     4321 atgccgtccg gcatcgtgcc gatggctaat aaccgccccg ctccgtcctt gatgccaatg
     4381 gcccgattcg ccccctcaga gagaggcgaa gacaagccat gccaggccag cacttctgag
     4441 gcaggaaccg gcatgccgtg caggacccgg ccggccgtcg ccgcccccac gatacaaacc
     4501 ggaagtccga ccagcgcctg atccaacgtc aacatcgacg ccgccagcgt gccttgctcc
     4561 aagcgtgatt cgacttcgtc caccgtcaac gcctgctcaa gggtcagtgg ccccacgcgc
     4621 tcgcggacca acgtcgccat atgaccgccg acccctaact tgtcgccgat atccgcgcag
     4681 agcgtcctga tatacgtccc cttggaacag accacccgga gcgtcacttc aggaatctga
     4741 acatccacga tatcgaggcg aaagacggtc acttcacgaa cctgccggga gacctcccgc
     4801 ccggctcgcg ccgctttgta taacggaacc ccgccgactt ttaccgcaga atacatggga
     4861 gggagttgct gaatgcgccc ctcaaaccca gctaccactt cacggatgcg ggcttcggac
     4921 aacgactcaa tcggtgaacg caggagcacg gtcccggtgg catcttgcgt gtcggttgtt
     4981 tcaccgaggc gcaatccggc gagataggtt ttgtcccatt ccagcaaata ttcagcgatg
     5041 cgcgttccac gcccgacgag caacggcagg acgccggtcg ccgccggatc cagcgttccg
     5101 gcatggccca acttcatgcc gcgcaacttc ccacgaatgc gagccaccac atcgtgcgac
     5161 gtccacccgg cttccttgcg gacattcaac actccgtcct gaagcacagt agtcgcgcgt
     5221 ggctccatcg aggcgatcat ggtcatttgc tagacatcgg tgtggggggg ctctgccaac
     5281 atctcttcgt gggattgatc gcggtgcagt ccatccaaca gctccaggac tcggtcagct
     5341 ctagggccac tgatgtcttt cataaagatc agctccggca ggtatcgtaa tgccagacgc
     5401 cgccccaact ccgcccgaac gaaaccgctg gccttcgcca gcccgtcgaa caccaaccgc
     5461 tcatccttat tctgctccat ggtcgtcaca aagacgcgcg cgatgcgaag atctttggtc
     5521 aactccacat cggtcacggt gaccgagcgc acacgcggat ccttaatctt ccgcatcaga
     5581 atgtcggcca cttccatgcg gatctggtct gccacacgat cggcccggct gtacgtcgat
     5641 ttggtcatga cgccgcctta cagcaactcg attcgcgagc gtactagttg gatcacaggc
     5701 acggactgaa tcaaattcac ggcttgatcc aacacctggt tcacatgcgc ggattcattc
     5761 gccacgcagg ccatacctaa aattgccttc tgccacaaat cctggtctcc gacttccgcg
     5821 accgatagat taaacttgtc tcgcaaccgg gtcttcaggc tttgcaggac ctggcgcttc
     5881 tctttcaacg aatgcccgtc gggaatgaac aactcaaccg tgcagagccc gacggtcatg
     5941 cccgatgact ttgcggagac gtgccacggt tcgcaggctc aagcttggcg gcaatcttat
     6001 caacgacgta ggcctcgata atgtccccgg ccttgagatc attgaagttt tcgaccgtga
     6061 taccgcactc atacccctgc tgcacttcac gcacatcatc cttgaagcga cggagcgagc
     6121 ccagcttgcc ttcataggcc accacactat cccgaatcac gcgcacgccg acgctggccc
     6181 gggagatgac gccatccacc acgtaacagc cggccaccag accggccttg gggatcgtaa
     6241 acatctgccg tacttcagcc cgcccaagga cacgctcctt gagcgtgggt tcgagtaagc
     6301 cctccatcgc ggcgcggata tcgttgagtg catcgtaaat aatgctgtac aggcgcacat
     6361 cgacgccctc ccgctcggcc aacgccgccg ccttcggctc cggacggata ttaaagccga
     6421 tcacaatggc cttggacgcg gccgccaaga gaatgtcggt ttcggtaata ccgcccaccc
     6481 cggtatgcat tacacgcaac ttgactgcgc cggccggcat tttttccacc gcggcagcca
     6541 aggcctcggc cgacccctgc acatcggcct tgatgacgat gggcaattct ttgacattgc
     6601 cttcctgaat cttggcaaac agatcatcca ggctcacctt cgccggccca gccagatccg
     6661 ccgcccgctg cttcatcgcc cgctcctgcg caatctcacg ggcaacccgt tcatctttca
     6721 cgatggtgaa caaatcgccg gccgacggca cgcccggcaa accgatcacc tcgacaggaa
     6781 cagacgggcc agcctcagtc gtcttgctcc ccgtgtcagt cacgagcgcg cgcacacggc
     6841 cgctgaaatt gccgactaca aatgcatccc cgacatggag cgtcccgctc tgcaccagca
     6901 ccgtcgcgat cggtccacgc ccgcggtcca acttcgcctc gatcaccagg cccttggcca
     6961 tgcgcgatgg atccgccttc aactccaaga cttccgcctg cagaagaatc atttccaaca
     7021 actggtccag gccggtccgt tgcttcgcgg acacctcaac cataatcgtg tcgccgcccc
     7081 agggctcagg aaccagccca tgctcggtca gggcattctt gacacgatcg atattggctc
     7141 ccggcttgtc gaccttattg atggcgacga tcaatggcac tccggcagcc ttggcatggt
     7201 gaatggcttc caccgtctgc ggcatgacgc catcatcggc agccacgacc agaatcacga
     7261 tgtccgtggc cttcgcgcct ctggcgcgca tggccgtaaa ggcttcgtgg cccggggtat
     7321 cgaggaaggt cacctgctta ccgcgaactc cgacgatata ggcgccgata tgctgcgtaa
     7381 tccctccggc ctcaccttcc gccacctttg tcaggcgaat cgcatcgagc aaagacgttt
     7441 tcccatgatc gacatgcccc atgatcgtga cgaccggagg ccgcaagacc gcatgctcct
     7501 cacccgatga ttgggcggct tcttccagca acgcttcgcc aaccttttct gtcgacactt
     7561 ccaccctggt gccatactct tcggcgatca aggaggcagc ctccagattg atcgactgat
     7621 tgaaggtcac catctgcccc atctccatca gcttccggac cacgtccgca ggccgctgac
     7681 cgatgagttc tgcgaattcc ttgacgctca ctcccgcgct caatttcacg cttttctttc
     7741 tcggcttggt gacttccgtt ggcgaggcat ggtgaatatg cttggatcgg tcttcgcgcc
     7801 gctgcaccgg gattgcccgc agatctcccc atcgagtggc gtcgttctta aacttggctt
     7861 catcttcctc acgggtccgc ggagccttct tggctttttt caatttttct cgagccgccg
     7921 cttcgctctc catggcctcc gtggccgcac tcttcttctt tgccgtcgcc agcgcatcaa
     7981 gcgggggagc cgtgacctgg ggaacctgga ccggcttgac gagcggctcc tccgaaacgg
     8041 tcacgacagg cggtggggac actaattctt cgggtggggc aggttcaacc ggggcggctt
     8101 caatcgcttc tgcgtcagga ccagccgccg gcgctaccac cgacgcttcg gttcctgcag
     8161 ggacgacggg cgcaaacgcc gcttctgccg ccgccagaga ggccgcaaca tcctccgcac
     8221 cttcttccct cttcttcttg atgagaatcc gtttcttgtc cggcttcggg ggctcttcgt
     8281 gcgccgccgc gtgtcccctc tcgtgagacg caccgggttc tttcgacgag cgaaccattt
     8341 tcttcgcctc atgcccgccg gagacagcct caccgccccg agccttcgag ctcagcttct
     8401 ccaagacaac gcgaaccgaa tcgtcatcca aggcgctgct gtgggacgcc accggaatcc
     8461 cgagccgttt cagttcagga atcagctccc gattttccat ccccagctgc tttgctaatt
     8521 cgtacacgcg catacagtac cgctcagctc cacccgctta aataattgcc attcagcccg
     8581 tgaatatcga accgcttcac tccgcctgtt gcgcagcagc ccgcgcttca tcctgctggc
     8641 gctgcgcctc ggtttcctct gccagggccg ccttgatatc ccgatcccgc tccgcctttt
     8701 ctttctcgta ttcggtcgcg ctgatgatat cgatcttcca accggtcaag cgcgcggcga
     8761 gtcgcacatt ttgcccgttc ttcccgatcg ccagagacaa ctgcgaatcg gccaccacca
     8821 ccagggccga cttcttttct tcgtcgatcc cgaccttctc gatcgtggcg ggattcagcg
     8881 cctcggcgat aaagacacgc ggatcctgtg tccacgtaat aatgtcgatc ttttccccgc
     8941 gcaactcacg aaccacagcc tgcacacgcg atcccttgat gccgacacaa gcccctaccg
     9001 ggtcgacagc cttgtcccgc gaggaaaccg caattttggt acgatcgccg ggctcacgca
     9061 cgatcgactt gatttcaacg atcttttcgc cgacttccgg cacttcgagc tcaaacatct
     9121 tcgccacaaa ctgcgggtgg ctgcgggaaa gaatgacctg cacgtccttg ggggtgcgac
     9181 gcacctccaa gagcagggcc ttcacccggt ctccgcgacg ataggtttcg cgcgggatct
     9241 gttcctgaat gggaagaatc gcttcggtct tgccgagatc gaccagaaag tttcggcgct
     9301 ccatgccgag aataatgccg ttcaccaaat caccctgacg ggtcgagtac tccttctgaa
     9361 cagcttccca ctcggcttcg cgcactttct gaaagatcac ctgcttggcc gtttgggccg
     9421 caatccggcc taactcgtcc atctcgatca atgagccgat ctcatcgcca acctccgcgc
     9481 cttcgtcata ttcgcgcgct tcctttagcg agatttccgc tttcggattg gccaccgtat
     9541 cgacaatgat tttctttgaa acgaccgaga tttcgccggt cttcggatcg atttccacct
     9601 ggatgttttc ggcctggccg aatcgtttct tggcagcagt ttgaagcgct gactcgatcg
     9661 cgccaatgac cctggcctta tcgatccctt tttgacggcc gatttcatcg atgacggcga
     9721 tcaactctcg gttcatagct cacacctcat ggttaacaca ccaccctggc acctgctgct
     9781 acgagaactc caccttccgc cgcgccagag cgatttgatc aaactcaact cggattgtct
     9841 ccgtggtttt cttctgctga atggcaaggg tcaccccccg gtcgtccacc tcgaccactg
     9901 cgccaaccag ccgccactgc gcctgaatag gttcgcacaa tttgagtgtg accggcttcc
     9961 cgatcagacg ccgatagtct tgcacgctcc gcaagggacg atccagcccg ggagacgaaa
    10021 tttccagcgt ataggcatgc gggaatggat caatcacatc gagcgccggg ctcaacgagc
    10081 gatgcgcctg ctcacagtct gtcagggtga tccctccagg cttgtcgata aaaacacgaa
    10141 tcacggtcct gggcccttgg ccgacgcaga cggcctcgac caactccaga ccataagact
    10201 gcaaaatagg agcggcaatt tcttggacgc gaaggttcag cgcctcagcg cgtttcaccg
    10261 acggcttccc tgccgacact gctcccgcgt cactcataga aaaaagttgc tcaaacaaaa
    10321 aagtgggcct tggcccactt acagcgcgag aaccttacca tgccatagta gtgaaagcaa
    10381 ggggcggcta gccgaccagc gcttcaaaac ggcgagacaa taactgagaa acagggccgg
    10441 gcgatccgcc cccaatcata tgtccgtcga cacgcaccac gggcaatacc tccacggtcg
    10501 ttcctgtcag aaatacctcc gacgccgcaa gtaattcttc ccgagtgacg aacgattccg
    10561 ccaccggaat accttctttg cgtgccagat ccagaacgat cgcgcgcgtc accccggaca
    10621 gaatcctgcg cccttccggc gccgtctgaa cggcgccgtt ccgcaccacc atgacattgc
    10681 tgaccgaccc ttccgtgacc gtcccctctc ggaccagaat cgcttcaaac acgcccgcct
    10741 ccttggctcg ctgccgcgcc aggacgttgg ccagaagatt gacgctcttg atgtcacagc
    10801 gtccccatcg aatgtcctcg accgacatag cctgaacccc ggcgttgcga acggagacat
    10861 ccagcgggtg gagttcgcga aaggtcagaa cggtggtggg ggcggacgat acagggaatg
    10921 gatgatcgcg gggagcctgc ccgcgagtga tctggagata aattttggct tcagggaatt
    10981 ggctcagccg caggccttct cgaatcaagg aactccactg agccctcgtt tgcccgattg
    11041 caagctgcag cgcctgagca ctccgctcca atcgaaccag gtgctcctca atggcgaagg
    11101 gctgtccacg ataggtccga atgacttcgt agaccccgtc accgaactga aagcctcgat
    11161 cctcaatact gacgaccgca tcagccagcg gaccaaagcg accattcaca tacgcaatgt
    11221 caggcatgac tcatttctca gcacgtttcg aacctcagga tacggtcacg taaaaaatct
    11281 gtcggtcctc agccgtgaac ttccacccca tccgcttttc aaacaccaac cggtgggttc
    11341 ctgcggccac aggcctgaat tcaaacgtac gctgccccgc gtcgaccgca ttgttactgg
    11401 cagtgcgaag aaaatcgtcc tcaaccagag gcatgacggt cgcgtcgtag gacggaaccc
    11461 acaactcccc gcgggtgcga tcctcccaca gccgaatgtg aaccgatgcc cccactctcg
    11521 ccttgagcgt cttggcgccc ggctcctgct ctgcaccggt gggaactgcc ttacttaatg
    11581 gactcatact attctcatgc tgccccgagg caatcactcg cccgactata ccttgcgccg
    11641 ccccccgaca aacacctgcc cgtcttccac ccgcacatca tagctcccga cacagtagcc
    11701 gccgccatcc gtgccttgcc catttcggac atcgaacgcc aggtcatgcc agggacagga
    11761 gacaacatgc cccttcaccc ggcccttatt taaaggcccg ccctcgtgag ggcaggaatt
    11821 gtagatcgcg tgaaattgac cgtcgatatt aaatacggca atgccccgct cgttgacctt
    11881 cacaaccttg acctgcccag gaggaatctc ttcgaccttg gccacgcact gaaacccttc
    11941 cataaacgac tccgattctt tacatacgaa gcgtaaactt gagttccatc cccgtcgtga
    12001 tgcctgcgcg ctggacagag cccgcttcag cctcgatgag gtagcgaacc ggttcgggcg
    12061 gaggcccata gagtggacac ggatccttct cgcagggcgg cacctgttcc aggatgtgga
    12121 ccacgtggtg gctctcatca atccacacca tgtcgacggc gatgcgaaac tgccgagtct
    12181 gaacgcgatg caaacccgtc gactcaaaga tataaagcat ccccgtatcg gctggcaatc
    12241 cttctcgaaa cgccagaccg aacaacaatt tctcaggggt gctggccact tccgtttcca
    12301 tggtctttcc gttgggaaat tccacgatga tgagctcgga ctccttcggc cccccaagaa
    12361 acaggctcac gctgaccaac aaaataacca agagcacgaa cgtcacaatt ttcttgcgac
    12421 gctgctccga ctcagccgca ctcgcgcgcc tcaccataac caaatgcggt cgaacatgga
    12481 tgcatcctca tgtgatcgca cacgcgcaga gccgcatgta cattgggccc aaagccccat
    12541 gactttccga gtgctaggcc ttcttcccga gcctcactct gttgacttgc cgaagaacgg
    12601 cggcatagaa tgcggacctg tttcgccgcg aa
//
LOCUS       DAOQRM010000073        12163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_4358_length_12163_cov_11.433598, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000073 DAOQRM010000000
VERSION     DAOQRM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4358_length_12163_cov_11.433598"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PKK81_07470"
     CDS             complement(<1..281)
                     /locus_tag="PKK81_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNV32752.1"
                     /translation="MTSENVPAVVARYFSAIGEMDPDAWAACFAEQGTSYEPGSPTPL
                     QGHAALRQFLAGVLGAFEKITMTQDHVFLSGNRVAVKFTGRGAGKNGRAV"
     gene            578..934
                     /locus_tag="PKK81_07475"
     CDS             578..934
                     /locus_tag="PKK81_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32753.1"
                     /translation="MIVEVESNDNCDTSFFARFKEVGPARPVVQVRLYERNPTGEWCW
                     VTGWSDNEQAPTCPAYVQLIEDSGSGLAYLVYGGLYGLRFRPIQFDEPWSLQSSNQWG
                     EAYLSLADVRDVRYAG"
     gene            1226..2083
                     /locus_tag="PKK81_07480"
     CDS             1226..2083
                     /locus_tag="PKK81_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV32754.1"
                     /translation="MRTVVAVDWSEQSFNAVKVVCRLFTHESLTLIHAVDLRPFENPL
                     FAQPLGSNSAGALREGMVAAGERLLDQTGALVPVTIPSIQRVYNIGNPAPVVLEAIRS
                     AHADLVAVGSRGRGRLAELVLGSVSHRVVLHAPCSALLVRDDPGSIRRVLLTVEGYED
                     SVRLRSWLSTHRFSRPIELSLLTVVPTIPIGDSTMEFRYDKWTAEMDQYARQLVDETA
                     AALRTMYPAVTAQVEHGDPAHCITQAAGHADLVIVGSHGWRGLEHFLLGSVSHAVLHK
                     VTCPILVIR"
     gene            2190..2612
                     /locus_tag="PKK81_07485"
     CDS             2190..2612
                     /locus_tag="PKK81_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV32755.1"
                     /translation="MAWNIASKIVHRVVTIDENHTVLDAATLMAEEFVGSALITSSSK
                     IAGVFTERDLMMRVVGRKRDPENVKIKDVMTKNMVTVSPKDTANHCLNLMKEHRCRHL
                     LVFDNEEFIGIVSLRDMVALLMEEKEALIAYLHQYISS"
     gene            complement(2694..3440)
                     /locus_tag="PKK81_07490"
     CDS             complement(2694..3440)
                     /locus_tag="PKK81_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV32756.1"
                     /translation="MNCTSCETALPLRARFCPACGVHVTLPEDPAFDEAALQAAGCGD
                     ELNQRTAPAPLDHEALKARLIDCYVNFSMARELSGWLQALGRPSTGTMQEKLTRVRQQ
                     ADSLVLPAESLPRQTIWYLNRYDEKILTEICEELGIDSSGPKERQITRIYHAVGLREG
                     WLQPLSEDARNIITETFLPILRAVDHRNYADLDQWGELGDLLDRESRTRLDHQGHGSA
                     FMAVLIPSLLQEAHRDLLQHELDARAEKPF"
     gene            complement(3503..4060)
                     /locus_tag="PKK81_07495"
     CDS             complement(3503..4060)
                     /locus_tag="PKK81_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV32757.1"
                     /translation="MTTKTTGDQPLFTPEQTFSSHTSAKNILIAVDDSDESARALHYV
                     GALLRDIQDVNVTLFHVLNPMPRELMEHGGSENPETEHHLGEQLRKDQEEWIRTEGAI
                     EYPILVTAMERLGQTGFPLDHVTLKLGHERDIADSIMDEARAGGYGTVVVTRHGPTGT
                     KRLFSSRITDRLVRDLSGVALWVLG"
     gene            complement(4386..4982)
                     /locus_tag="PKK81_07500"
     CDS             complement(4386..4982)
                     /locus_tag="PKK81_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32758.1"
                     /translation="MALVQQNLVPATGDHLVTLDTETNLEWLSLNATANLSYLEVLGG
                     AGGYTTTYGFRYATGQEIGLLWQHAGITKYGVTHVVPFPQSNHVAMETLIELMGGATL
                     YPSVTSGSVLVQTQGMMKFRGAGVPTPITPMSVGQLWLFKNNPGGSYADTNPAGNAGK
                     RTPEIASYLVRDRIMPAGSISRGKSAKAVKARSAKKQG"
     gene            complement(5435..5992)
                     /locus_tag="PKK81_07505"
     CDS             complement(5435..5992)
                     /locus_tag="PKK81_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32759.1"
                     /translation="MALLQQDLVPASGDSLITFDTDTNLEWLSLTKTVNLSISDVRNG
                     TGGYATTYGFRYANGAELQALWNHAGITRFAPNQPVPLPDSNSAGIQKLIDWMGGATS
                     YPTTGTIQTQGIFMVPPAPGHPGVGQLWFFTNNPGGSYATTDIFPNVPQGVTPEDYRS
                     SALASYLVRNHVAPTPPRNLRVGEK"
     gene            complement(6067..6498)
                     /locus_tag="PKK81_07510"
     CDS             complement(6067..6498)
                     /locus_tag="PKK81_07510"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNV32760.1"
                     /translation="MDLRTTFPRSMRVRLEGYVHLARMIDKCRAVLAGTEGEYIYPCP
                     MDDRLMAFAGITAAQFTAAVKQHLSDEGVIQWFRGMATPHAQAELEEWNRMMLSRGPT
                     TPEKRASFNKYRDAVDPSRTDLTSWAELQDLEEGRTVPRRA"
     gene            complement(6498..7175)
                     /locus_tag="PKK81_07515"
     CDS             complement(6498..7175)
                     /locus_tag="PKK81_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 domain-containing protein"
                     /protein_id="HNV32761.1"
                     /translation="MEHAAAIDTQTTVADNPQARALLQRAFESTARWQPDFRGFSADL
                     TVNTNGQQVRGTVVVKGPREVTVQLPDETIQKWAQEQIGMIAVHRAPRKFEESDGKHR
                     LTMEAGEDHPLGRRLDIHGDGMQSFYRIKDNRITQINRKMPHVAFTINVEESSTTQDQ
                     KQLTTKYTVYYFAPQDGKLRNVESFTDTHVRIGNSDLPSTRRIISYENGAVLVRTLTF
                     TNHKLLA"
     gene            7423..7770
                     /locus_tag="PKK81_07520"
     CDS             7423..7770
                     /locus_tag="PKK81_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32762.1"
                     /translation="MERRAASHTEEEEMNQRRRLLGLARKGDPKAISKLFELYQVRVL
                     NGDMLSKLNKSYYKAAAAQEQKASQASSKPAKMAKGAHGSAKSSPKPSTSQPVAASKP
                     KTTKATGAKKPAK"
     gene            complement(7820..8113)
                     /locus_tag="PKK81_07525"
     CDS             complement(7820..8113)
                     /locus_tag="PKK81_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNV32763.1"
                     /translation="MSTLTPAMTPEALDTTDVGTGDDLEARVIVFNCDCHTYQQVIAL
                     FCTCIPGMNSSRAFELAWRIDHEGQATVYSGERKAAEEIGKKLAAGGLRVGVQ"
     gene            complement(8228..8944)
                     /locus_tag="PKK81_07530"
     CDS             complement(8228..8944)
                     /locus_tag="PKK81_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32764.1"
                     /translation="MNALSPRNALFHPFHLCAPHTLEALLARYDAVHFRDYMALRLTP
                     LMGTTAYQDRMGDDHPMLVTSGRLVQGYPVSGPLDDTAVTAIDRDLSDSRWRTLFHDG
                     LRNDRRFQRGLFDLTHAMRIGSSLVPGPAALLRLLEPNRAAALYNVALVQRLAKPTLT
                     LDEAYQFEYGLALLKTAAAQVYTIRLSRAHNLVPVTDSHTHHVLLSRTLAREGIDLAH
                     ESIGASVGAPLSQHTSGLHE"
     gene            complement(9044..9241)
                     /locus_tag="PKK81_07535"
     CDS             complement(9044..9241)
                     /locus_tag="PKK81_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32765.1"
                     /translation="MPVNMDPTKRRRRQSAEQAAAAADQDSSQIKRFGEDTEADREKA
                     IAEAELKNLWDATEVIDMDTW"
     gene            complement(9311..10462)
                     /gene="hemW"
                     /locus_tag="PKK81_07540"
     CDS             complement(9311..10462)
                     /gene="hemW"
                     /locus_tag="PKK81_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNV32766.1"
                     /translation="MRQATGRGLYIHVPFCHQRCHFCAFYLEIHHARAAEEFLGSLTT
                     ELRLYGEQRLFGSEPLQSVYFGGGTPTTLSAPQLATVLSAARETFGLQDEAEITVEAH
                     PGSVTRDSLSHLRRAGFTRVSFGAESMNQLELDRVGRPGSPMSTVQMATAARQAGFTD
                     LNLDLMYGLPGQTIESWEASLRRIVDLAPTHLSCYALTVEEGTALKAAIRRGTVPAPD
                     ETLQNEMEDLAEDVLSRAGFTRYEISNYCRPGFASRHNQLHWTGGDYLGLGPSAQSYV
                     AGRRFGNVSDLSAYNASLRTGTLPVTASEQLTTADDACERLVFGLRLTEGITLGETGA
                     LAFSGLTGKVNELIGDDILERRGERVRLTARGRRYADTVAVALLASLEP"
     gene            complement(10477..10731)
                     /locus_tag="PKK81_07545"
     CDS             complement(10477..10731)
                     /locus_tag="PKK81_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32767.1"
                     /translation="MWSEDTSFVFRISLEAQFPDDYEGPHDEHAWLREWELQIKPVLI
                     KNLFETLREYPAWKAHVRNRGKSPTEEIEVAMTRDFSTAP"
     assembly_gap    10864..10963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11124..11813
                     /locus_tag="PKK81_07550"
     CDS             11124..11813
                     /locus_tag="PKK81_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32768.1"
                     /translation="MIRFDRGLRLLMTLLLLGSLSPVSTRAEESAGVRPLRNGLAVAL
                     ASLPAQTHLLLDQQSIEQFLVELDGAPPDWATVYGQGHHDPGHDERLFALNRERDARR
                     EGKAPLEWLIAFVWLGELSGFDEREGGFRVVLGPKFNRTAWGEVRFKHEDLPSTLIAL
                     AGQGTAKLRTRIQQGTPAPVAVLITGRLIPEESLMYDFSHEAEGLGLIMPVVRVESVD
                     FVLEGGQEKQP"
     gene            complement(11825..>12163)
                     /locus_tag="PKK81_07555"
     CDS             complement(11825..>12163)
                     /locus_tag="PKK81_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32769.1"
                     /translation="AYTCGATDYIRKPVIPAELIARAANALSLKQEIDARKLREQELL
                     ERTKELDHAFEQITTLHGTVHICAKCKRVKADGPHWQRVEDYLRRQARAKVAETVCDT
                     CLHQAYPHLK"
BASE COUNT         2281 a         3379 c         3734 g         2669 t
ORIGIN      
        1 actgctcggc cgttcttgcc ggcgccacgc cctgtgaact tcaccgcgac ccggttgccg
       61 gagaggaaga catggtcctg cgtcatagtg atcttttcaa aggccccgag aacccccgct
      121 agaaattgtc gcagtgccgc atggccttgg aggggtgtgg gggagcctgg ctcgtaactg
      181 gttccctgct ctgcgaagca ggcggcccat gcgtccgggt ccatctctcc tatggcgcta
      241 aaataccggg ccacaactgc cggaacgttc tcagaggtca tgcttgattc tcctttgcat
      301 cggatggaat gttgtgtgaa taaacatgct ggaaactctg gccttcagat gaattacgga
      361 ggcgtagcat cctgtggatt tcttattcct gaaaatttct tcgccggcac agcgacgact
      421 tagcctgagg agcatcctca ggcgtagccc tgaagatgat gcctatgctg gtggcaacag
      481 atcaacctat gattgaattg tgcggcccct cggtaagggc aatgtttcag tcggccatcc
      541 tccgcgatga atttaccagc agttgcatga ggacctcatg atcgtggaag ttgaatcgaa
      601 cgacaattgc gacaccagct tcttcgcgcg cttcaaggaa gtcgggccgg ctcgaccggt
      661 ggttcaagtc cggctgtatg agcgaaaccc taccggtgag tggtgctggg tgacgggctg
      721 gagcgacaat gaacaggctc ccacatgtcc cgcctacgtc cagctgattg aagattcggg
      781 gtcggggttg gcgtacctcg tgtatggagg gctctatggt ctacggttca ggcccatcca
      841 attcgatgag ccttggagtc tccagagctc caatcaatgg ggagaggcgt atctgtccct
      901 cgccgatgtc cgtgatgtgc gttacgccgg ttagctgtct ttgcaaacag ggaggtgatc
      961 cggtcccggc ggcatactcc tgttcccatt cacgactcgg gcgtgcaata gaataagcac
     1021 gctgcgatat tcgatgggtt tctctccatt ggccagctcc gtcccgagcc ctaccagccg
     1081 aatattttcg ctgcgcttga cctccatcgc cggataggga gtatgatggc gcatcctgca
     1141 atctgcggat gtcgctctac tgaatgaggg ttctctgcct cctagaagag cgattgccga
     1201 cacgccttcg cttcaggagg ctgcgatgcg caccgtcgtt gccgtcgact ggtccgagca
     1261 gtctttcaac gccgttaaag tcgtctgccg tctcttcact catgaatcat tgaccctcat
     1321 ccatgcggtc gatctccggc cgttcgaaaa tccgctcttt gcgcaaccgc tcggcagcaa
     1381 cagcgctgga gcactgcgcg aggggatggt tgcagcagga gagcgcctgc ttgatcaaac
     1441 cggcgcattg gtgccggtca caattccttc catccagcga gtctataaca tcgggaaccc
     1501 ggcgccggtt gtgctggagg cgattcgttc ggctcatgcc gacttggttg cggtcggatc
     1561 acgggggcgt ggacgccttg ctgaattggt cttaggcagc gtctcgcatc gagtagtgct
     1621 gcatgctccc tgctcggccc tgctggtcag ggacgacccg gggagcattc gacgggtgtt
     1681 gctcaccgtg gaagggtatg aggattcagt caggctgcgc agctggttga gcactcaccg
     1741 gttcagccgg ccgatagaac tgtccctctt gaccgtcgtg ccgaccattc ccatcggcga
     1801 ttcgacgatg gaatttcgat acgacaaatg gacggctgaa atggatcagt atgcccggca
     1861 acttgtggac gaaacggctg cagccttgcg aaccatgtat cctgctgtca ccgcgcaagt
     1921 cgagcatggt gatccggctc attgcattac ccaagcggca ggccatgccg atcttgtgat
     1981 tgtcggatcg catggttgga gaggcctcga gcacttcctg ctcggcagcg tgtcacatgc
     2041 ggtcttgcat aaagtgacct gtcccatcct ggtcattcga taaagggggc tctgactccg
     2101 gtttcgatgg gcgtcggcgt tacccggccc ctgtacttgg cataaagcac aggctccgtc
     2161 cacctgcatc caatagaaag gaggcggcga tggcgtggaa tattgcctcg aaaattgttc
     2221 acagggtggt gacgatcgac gagaatcata ccgtgctcga tgccgcgacg ctgatggccg
     2281 aggaattcgt cggatcggcg ctcatcacca gttcctccaa aatcgccggc gttttcacgg
     2341 aacgagactt gatgatgcgc gtcgtcggtc ggaagcgaga tccggagaac gtaaagatca
     2401 aggatgtgat gacgaaaaat atggtcaccg tgagtccgaa ggatactgcg aatcattgtt
     2461 tgaatctgat gaaggaacat cgctgccgcc acctcttggt gttcgacaac gaggagttta
     2521 tcgggattgt ctcgctccgg gatatggttg cgctgttgat ggaggaaaag gaggcgctca
     2581 tcgcctatct ccaccaatac atcagttcat gaagactgcg acggtcggtt cggcagagag
     2641 gagggctttc gggcctctgc tgtcagagac ccgaggggac cggtgttgtg tcgtcagaag
     2701 ggtttctcgg ctctggcatc gagttcatgt tgaagcagat cccgatgcgc ctcttggagt
     2761 aagctgggaa tgaggacagc catgaaggcg ctcccatggc cttgatgatc gagccgagtc
     2821 ctactttccc tgtcgagcag gtctccgagt tcaccccact ggtcgaggtc agcataattt
     2881 ctgtgatcga ccgctctcag gataggcaga aacgtctcgg tgatgatatt tcgagcatct
     2941 tccgacagcg gttggagcca gccctcacgc agacccacgg catgatagat cctggtgatc
     3001 tgccgctcct ttggtccgct gctgtcgata ccaagctctt cgcaaatctc cgtgagaatc
     3061 ttctcgtcat atcggttcag gtaccagatg gtctggcgag gaagactttc ggccggcagt
     3121 accagcgaat ccgcttgttg tcgcacacga gtgagtttct cctgcatcgt tcccgttgat
     3181 ggacgcccga gtgcttgcag ccatccggat agttccctgg ccatggaaaa attcacataa
     3241 caatcgatca gccgtgcctt caaggcttca tggtcgaggg gcgccggagc cgttcgttgg
     3301 ttcaactcgt ccccgcaccc ggccgcttgc aaggctgctt catcgaacgc aggatcctcc
     3361 ggcaatgtca cgtgcacccc gcaggcggga caaaagcggg cccgcaacgg gagcgcagtc
     3421 tcacaactgg tgcagttcat ggtagcctcc gaggcgcgcc taacgcgcat acttcaggac
     3481 gatagttgcg cctgtcacaa gattatccca ggacccagag cgcgactccg gacaggtccc
     3541 gcaccaggcg gtcggtgatg cggctgctga agagtcgctt ggttcccgtc ggtccgtgtc
     3601 gggtcaccac gacggtgcca tatcctccgg ccctggcttc atccatgatg ctgtcggcga
     3661 tatcgcgttc atgtcccaat ttgagcgtga catggtcgag aggaaatccg gtctgcccga
     3721 gtcgttccat cgccgtcacc aggatgggat attcgatggc gccttcagtg cgtatccatt
     3781 cttcctggtc cttgcggagt tgttcgccga gatggtgttc agtctcaggg ttctccgagc
     3841 cgccgtgctc catcagttcc cgaggcatgg ggttcagcac atgaaacaat gttacattca
     3901 cgtcctgaat gtcccggagc agcgcgccga catagtgcag ggcgcgggcg gactcgtccg
     3961 agtcatcgac ggcgataaga atgttcttgg cagaggtgtg gctgctgaac gtctgttccg
     4021 gtgtgaaaag gggttggtca cccgttgtct tggtcgtcat agtatcacca tgcgcgtgca
     4081 ggagtagact gcctcactgt gtacccgagg gggcaggtta gttgtctggg cgatgatggt
     4141 gatctggcgg actgaggtct tccatacgac ccctcttgcg gtgatacggt gatggtcgcc
     4201 ggtggcggag cgagtatgcc cgtgaggagc taccatctcc cgcgatcagg acacgtggta
     4261 gcctatccct ctacggagga caaatcaatg tggggggcgc agacgggttc gccaaagggc
     4321 tggagtaaat ggaagcctgt gctccatgaa gccgatgatc ttcggcttca tggagctgct
     4381 tgagatcagc cctgcttttt cgcgctacgg gctttcaccg ccttggcgga ttttccacga
     4441 gagatggagc cggcgggcat gattcgatcg cgcaccagat aggaggcgat ttcaggagtt
     4501 cgttttccgg cgtttccagc cgggttggtg tctgcatagg acccgcctgg attgttcttg
     4561 aaaagccaga gttgcccgac gctcatgggg gtgatgggag tcgggacgcc cgctcctcga
     4621 aacttcatca tgccctgtgt ctggaccagg acggaaccgc tggtgaccga tgggtacagc
     4681 gttgcgccgc ccatgagttc gatgagggtt tccatcgcca cgtgattgct ttgagggaac
     4741 ggcaccacat gggtgacgcc atactttgtg atgccggcat gttgccagag cagtccgatt
     4801 tcctggccgg tggcataacg aaagccatag gtggtggtgt accctccggc gccacccagc
     4861 acctccaggt aggacagatt cgccgtcgca ttcaggctca accattcaag gttggtttcg
     4921 gtgtcgagtg tcaccagatg gtcgccggtt gcgggaacaa gattctgttg aacaagagcc
     4981 atgtcggtac ctcctgcgga tggtggtgga gtgggcgatt gcgcgaggtc tatcgtcagg
     5041 gtctatgcga tacatatgcc agtacgtctc aatgccatca tttcctaatt gtgtgaccat
     5101 cccgagtgag gcacgtagcc ctggcggcaa gatcacgggg cagcaataaa gcgcgtcgta
     5161 agtaacaaat aatcgtgaac aacacgtatc gggggagata cagccggaat ctgtatggat
     5221 acgattcgac ccatccgtcg gattcccgct gcgtcgtgcc ggctcttcac tgctgcggtg
     5281 aactgtcctg cctcgaagcc cacgctccat gaagccgatt ccgtccggct tcatggagct
     5341 ggcaggttgc tgtcgatgaa acggccttct ccgcgtcatg gtcttcatcc ctgatgcgca
     5401 gagttgccat aaaaaacgag tcgatggcca cgccttactt ttcgcccacc cgaaggttac
     5461 ggggaggagt aggggccacg tgattgcgta ccagatacga tgccagcgca gaactccggt
     5521 agtcttccgg cgtcactcct tgaggtacat tgggaaaaat atccgtggtt gcgtacgacc
     5581 caccagggtt gttggtgaag aaccagagtt gtcccacccc cgggtggccg ggtgctggtg
     5641 gaaccatgaa gattccttgc gtctggatcg tgccggttgt cggataactc gtcgcgccgc
     5701 ccatccagtc gatgagcttt tgtattcccg cggagttgct atcgggcaat ggtacaggct
     5761 ggttgggggc aaaccttgtg attccggcat ggttccacag cgcctggagc tcagcgccgt
     5821 tggcgtaacg aaaaccgtag gtggtggcgt agcccccggt gccgttccgc acgtccgaaa
     5881 tggaaagatt tacggtcttc gtgaggctca accattcgag gttggtatcg gtgtcgaacg
     5941 taatgagcga gtcaccgctt gcaggaacaa gatcctgttg taacagggcc atgttgtgcc
     6001 tcctatgaaa atgtaggacg agtctgctac cgacgcgcag gcggagcaga tgtcaaccat
     6061 tgccccctag gctcttcgtg ggaccgtccg cccttcttcg aggtcctgga gctcggccca
     6121 gctggtgagg tcggtgcggg agggatccac ggcgtcccgg tacttattaa atgaagcccg
     6181 cttctccggc gtggtcggcc cccggctcaa catcatccgg ttccactctt ccagctcggc
     6241 ttgggcgtgt ggcgtggcca tcccgcgaaa ccattggatg acgccttcgt cactcaggtg
     6301 ttgcttcacg gccgcggtga actgcgccgc ggtaataccg gcgaatgcca tcagccggtc
     6361 atccatgggg cagggataga tatactcgcc ttcggttccc gcgagcacag cgcggcactt
     6421 gtcgatcatg cgcgcgaggt gcacatatcc ttccaatcta acgcgcatgc tgcgcgggaa
     6481 cgtcgtgcgc aaatccatca ggccagcaac ttgtggttgg tgaacgtcag ggttcgaacc
     6541 aggaccgcgc cgttttcata cgaaatgatc cggcgtgtgg acggcagatc ggagttgccg
     6601 atccgcacat gggtgtcggt aaagctctcg acgttgcgta gcttgccatc ctgcggcgcg
     6661 aaataataga ccgtgtattt ggtggtcagc tgtttctggt cctgcgtggt gctgctttct
     6721 tccacgttga tggtgaacgc cacatggggc atcttccgat tgatctgcgt gatgcggtta
     6781 tctttgattc ggtagaaaga ctgcatgccg tcgccgtgga tgtctaaccg acggccgagc
     6841 gggtggtctt cgccggcttc catggtgagg cggtgtttgc cgtccgattc ctcgaacttg
     6901 cggggcgcgc ggtgcacggc gatcatgccg atctgttcct gggcccactt ctgaatggtt
     6961 tcgtccggca actgcaccgt gacctcacgc ggtcccttga ccaccacggt tccgcgcacc
     7021 tgctggccgt tggtattcac cgtcaggtcg gccgaaaatc ctcgaaaatc aggttgccat
     7081 ctggccgtgc tctcgaaggc ccgttggagc agggcgcggg cttgggggtt gtcggcaacg
     7141 gtggtttgtg tatcgatcgc tgcagcatgt tccatcaggt gctcctttgt gtcgtggtga
     7201 aggctaaaag ctgattatag agacctcaat cccccctcta caacccgagg cctgatcggt
     7261 ggaccggatc ggatcgaaca ggccagggcg cgatattttc tcccggatga ccgatgtttg
     7321 tgtagtactg gctaggccga atctgttata ctgcggggca tttcatccgg caatttggcg
     7381 tatttgggca gtgagtttcg ggcatccttt gaaaggagcg ctatggagcg gcgagcagcg
     7441 tctcatactg aagaagaaga aatgaatcaa cggcggcggc ttctgggact ggcgcgcaag
     7501 ggcgacccca aggccatcag caagctgttc gagttgtacc aggtgcgtgt tctgaacggc
     7561 gacatgctca gcaaacttaa taaatcctac tataaagcgg cggctgcgca ggaacaaaag
     7621 gccagtcagg cgagcagcaa accggcgaag atggccaagg gtgcgcatgg cagcgccaag
     7681 agcagtccga agccctccac atcccagcct gtagcagcca gcaagcccaa aaccaccaag
     7741 gcaacagggg caaagaaacc agccaagtaa agtcggtctg cgtcgagatc ctcggcgcag
     7801 accagccccg ttcccttctc tactgcactc cgacccgtaa ccctccggcc gccagtttct
     7861 tcccgatctc ctccgccgct tttcgctctc ccgaatagac cgtcgcctgg ccctcgtggt
     7921 cgatgcgcca cgccagttcg aaggcccggg aagagttcat tccaggaatg catgtgcaga
     7981 agagcgcgat gacctgctga taggtgtggc agtcgcagtt gaacacgatg acccgtgctt
     8041 ccaggtcgtc gccggtgccc acgtcggtcg tgtcgagggc ctcaggggtc atggcagggg
     8101 tcagtgtgct catgcggtcg tctcgccagg cgcggagtgt agcaggtgag atagggctga
     8161 atgcaaccgg aactcatgac agcggtagat cagtggcatc aagctccttg agtatcccaa
     8221 ctctggttta ttcatgcagc cctgatgtgt gctgcgacaa tggtgctccc acggacgcgc
     8281 cgatagattc gtgggcgagg tcgatcccct cacgggccag ggtccggctc agcaggacat
     8341 ggtgggtgtg ggagtcggtg accggcacca ggttgtgcgc gcgggacagg cgaatggtat
     8401 agacttgcgc ggcggcggtc ttcaacaagg ccagaccata ttcaaattga taggcctcgt
     8461 ccagcgtcag ggtcggtttt gcgaggcgtt gcacgagggc gacgttgtag agcgcggctg
     8521 cacggttcgg ctcaagcagt cggagcaacg cggccgggcc gggcaccagc gacgatccga
     8581 tcctcattgc atgcgtcagg tcgaagagcc cgcgctgaaa acgccggtcg tttctgagcc
     8641 cgtcatggaa cagggtgcgc caacgggaat cgctcaggtc acgatcaatg gccgtcacgg
     8701 cggtgtcgtc cagcgggccg ctgaccggat agccttgcac cagccggccg ctcgtgacga
     8761 gcatcggatg atcgtccccc atgcggtcct gataggccgt ggtgcccatc agcggagtga
     8821 gacggagcgc catgtagtcg cggaaatgga cggcatcgta ccgtgcgagc agagcctcaa
     8881 gggtgtgcgg ggcacagagg tggaacgggt gaaagagagc gttgcggggg gacaaggcgt
     8941 tcatggatcg tatggctgtc gatcgaaggt gagatcggat cggtgtgtgc tcgagtggtg
     9001 cgcgggactg gttgtatcga tctctgctgg ttacggcgct caatcaccag gtatccatat
     9061 caatgacttc ggtcgcgtcc cagaggtttt tcagttccgc ttctgcaatg gccttttccc
     9121 gatcggcttc ggtatcttct ccgaatcgct tgatctggct gctgtcctgg tcggccgcgg
     9181 ccgcagcctg ttcggcggac tgacgtcggc gccgcttggt ggggtccatg ttcactggca
     9241 tgacggaggc ctcctttcag ttcgtcagtc tcccctctgt cgggtcccga agtcaatggg
     9301 aggaggatag ttagggctcc aggcttgcca gcagggctac tgcgacagta tccgcatagc
     9361 gccggcctcg ggcggtgagt cgtacccgct cacccctgcg ttcgaggatg tcgtcgccga
     9421 tcaattcgtt tactttgccg gtcaaacctg agaatgccag ggctcccgtt tccccgagag
     9481 tgatgccctc ggtcagccgg agtccgaaca ccagtcgttc gcaggcgtcg tcggctgtcg
     9541 tgagttgctc ggatgctgtc acggggaggg tgccggttcg gagagaggcg ttgtaggcgc
     9601 tcagatcact cacatttcca aaccgccggc ccgcgacata ggattgggcg ctggggccca
     9661 ggccgagata gtctccgccg gtccagtgca actgattgtg tcggctggca aatccgggac
     9721 ggcagtagtt ggagatttcg tagcgcgtga agcctgccct gctcagaacg tcctccgcca
     9781 gatcttccat ctcattttgt agcgtctcgt caggggcggg aaccgtccca cgccggatcg
     9841 ccgccttcaa ggcggtgcct tcttcgaccg tcagggcata acaggagagg tgggtcgggg
     9901 cgagatcgac gatccggcgc agcgaggcct cccaactctc gatggtttgt cccggtaaac
     9961 cgtacatgag gtcgaggttc agatcggtga agccggcctg ccgcgcagcg gtcgccatct
    10021 gcacggtgct cataggactg ccggggcgtc cgactcgatc caattcgagt tgattcatgg
    10081 attcggcgcc gaagctcacc cgcgtgaatc cggctctgcg gaggtgcgac aagctgtccc
    10141 gcgtgaccga tcccgggtgg gcctcgacgg tgatttcagc ctcatcctgg agtccgaacg
    10201 tctccctcgc cgccgacagg acggtggcga gttgcggagc ggacagggtc gtgggcgtcc
    10261 cgccgccgaa gtagaccgat tgcaacggtt ccgatccaaa gagccgctgc tcaccataga
    10321 ggcgcagttc ggtcgtcagg gaccccagaa attcctccgc ggcacgcgcg tgatgaattt
    10381 cgagatagaa ggcgcagaaa tggcagcgct ggtgacagaa gggcacgtga atgtagaggc
    10441 cgcggccggt cgcctgacgc atggctgttc gtccggttag ggtgccgttg aaaaatcccg
    10501 ggtcatcgcg acttcaattt cttccgtcgg cgatttcccg cgattgcgga catgcgcctt
    10561 ccacgcggga tattctcgca gcgtctcgaa caggttcttg atgagtactg gtttgatctg
    10621 gagttcccac tcacgaagcc acgcatgttc atcgtgaggg ccttcatagt cgtcggggaa
    10681 ttgcgcttcg agactgattc gaaagacaaa cgatgtatct tctgaccaca tctgtggact
    10741 cctatcggct gatgattgcg aactggaacc gaccttactt ataatattct aaatctcaag
    10801 gagtacagca ccatgcccat attcgagtac gtctgtaagg aatgcaacca tcggtttgaa
    10861 ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttgaagt cccagccggt
    10981 ccctgccgcc tcattgcctc ggctggaccc agcgccccgg cttcaggtgc tggtcgatat
    11041 gggtgtgttg tcgtgggacc atcctggaac aaaagacctt cgcgacctct ttcagcgggt
    11101 gtcggtaccc gccgcctgag cccatgattc gcttcgacag agggttgcga ctcctcatga
    11161 ccctcctgct cttgggctcc ctgagtcctg tctcgacgcg agcagaggag tcggccggtg
    11221 tcaggccgtt acgcaacggc ctggctgtcg ccctggcgag ccttcccgct cagacccatc
    11281 tccttctcga tcagcaatcg attgaacagt ttcttgtgga gctggacggc gcgccgcccg
    11341 attgggccac tgtctatggg caggggcacc atgacccggg ccatgatgaa cggctctttg
    11401 cgctcaaccg cgagcgcgat gcccggcgtg aagggaaggc accgctagag tggctgattg
    11461 cgtttgtctg gctcggcgaa ctgtccgggt tcgatgagcg ggagggggga ttccgcgtcg
    11521 tactggggcc gaagttcaac cgaaccgcat ggggtgaggt gcggttcaag catgaggacc
    11581 tgccgtccac gctcattgcg ttggcggggc aggggaccgc caagctcaga acgcggatcc
    11641 agcagggaac gcctgccccc gttgcggtcc tgatcaccgg tcggctgatt ccggaggagt
    11701 cgttgatgta cgacttctcc catgaagcag aggggctggg actcatcatg ccggtggtgc
    11761 gtgtcgagtc ggttgacttt gtgctggagg gcggacagga aaagcagccg tgaccgggac
    11821 gtctttattt caggtgggga taggcctgat gcagacaagt gtcgcacacg gtttcggcga
    11881 ctttcgcgcg ggcctgccgg cgtaggtaat cttccacgcg ttgccaatgc gggccgtcgg
    11941 cttttacgcg tttgcacttg gcgcagatgt ggaccgtacc atggagcgtg gtgatctgct
    12001 cgaacgcgtg gtcgagttcc ttggtccgct ccagaagttc ctgctcacga agtttcctgg
    12061 catcgatctc ctgtttcagg ctgagcgcat tggccgcccg ggcgatcagt tcagccggga
    12121 tcaccggttt ccgaatatag tccgtggcgc cgcacgtata ggc
//
LOCUS       DAOQRM010000074        12138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_4373_length_12138_cov_16.516759, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000074 DAOQRM010000000
VERSION     DAOQRM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12138
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4373_length_12138_cov_16.516759"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PKK81_07560"
     CDS             <1..696
                     /locus_tag="PKK81_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNV32770.1"
                     /translation="PGLMFNAVSYDPRKEGVYQAVFRLLDELIDALHPRAIHIGHDEA
                     FGWTVGQVAKWLKFGELMVPADLFARDVERIHGYLTQKGVETWMWADMLLDPAEFPGA
                     SPKHLHGVAAGYGKALRNRLPRDIVMCDWHYGDEQGSFPSMAVMQSEGFRVIGATWKR
                     EETMRNFSRYALSRHAYGLMATTWFHVQRNDRDLVDWIIRSSGMLFRNPDAAVPPRPA
                     SAAPPEGHGWTAP"
     gene            complement(787..1479)
                     /locus_tag="PKK81_07565"
     CDS             complement(787..1479)
                     /locus_tag="PKK81_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNV32771.1"
                     /translation="MPRSLPLSLSLSLNLLLIGITLVGWVAPNVPNTFNPHVAVPTIA
                     ASARIHPLAAVIGSVTIGELIFIAPGASVRGDEGQNIYVGDHSNVQDGVVIHGLETFE
                     GDHELPENEVQVSGKTYSVYIGKRVSLAHQSQVHGPARIGNDTFVGMQALVFRTELGD
                     HVVVEPGAKLIGVKVASGRYVPALTLVTTQEQADTLPLITSAYPYRNLNEGVVTVNVQ
                     LAKAGQPKQIGR"
     gene            1889..2980
                     /gene="ychF"
                     /locus_tag="PKK81_07570"
     CDS             1889..2980
                     /gene="ychF"
                     /locus_tag="PKK81_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNV32772.1"
                     /translation="MSVKCGIVGLPNVGKSTLFNALTKSGIAAENYPFCTIEPNIGIV
                     EVPDARMQALADIVKPQRMQYATTEFVDIAGLVAGASKGEGLGNQFLATIRETDGIVN
                     VVRCFEDDNVVHVAGKVDPISDIATIVTELALADLTTVEKAQERNVKLVRSGDKDAAK
                     LGELLVQVAACLNEGKPARTLKLDPAQRALLKPLCLLTMKPVMYVANVAEKGFANNPL
                     LTRVEEYAAHEGAPVVAICAALESEIAVLSDEEKREFLADIGMTEPGLNRLIRAAYQL
                     LGLQTYFTAGVKEVRAWTIHIGDTAPQAAGVIHGDFEKGFIRAEVIGYNDFIACKGEA
                     GAKEKGKMRLEGKEYVVQDGDVMHFRFNV"
     gene            3038..>3368
                     /locus_tag="PKK81_07575"
     CDS             3038..>3368
                     /locus_tag="PKK81_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNV32773.1"
                     /translation="MNEQDTQRAVAILNRIMEHELAGVVRYTHYALMVYGYNRIPIVS
                     WLKGNADESLAHAHKAGELVTLLGGHPSLKIGTLLETEKHDVGDILRESLEHEKAAVA
                     SYYELLKL"
     assembly_gap    3369..3468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3469..3891
                     /locus_tag="PKK81_07580"
     CDS             <3469..3891
                     /locus_tag="PKK81_07580"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNV32774.1"
                     /translation="TGNIIRLCANTGASLHLVKPLGFTLEDKQLLRAGLDYHEFASIT
                     VHEGWAECLARMEGRRIFAVSTKGRQRYDLVRYIEGDAFVFGPESRGLPAEILGSVPE
                     QQCLRVPMVPGSRSLNLSNSVAVVLYEAWRQVEFWKGV"
     gene            complement(3966..5849)
                     /locus_tag="PKK81_07585"
     CDS             complement(3966..5849)
                     /locus_tag="PKK81_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNV32775.1"
                     /translation="MPKHLNNQSSSVSLTVAAMGVVYGDIGTSPLYALRECFHESHGL
                     PVTPDNVLGILSMIVWSLVLVVTVKYLLFVMKADNEGEGGMLALLALSQQSRPVDLRK
                     GLNVVVTLGLIGVAFLYSDGIITPAISVLSAVEGLTLTTDVFTPYILPLTVALVALLF
                     MIQRRGSGTIGSIFGPIMLLWFVTMALLGLHSLIQTPEVLRAANPLYAIQFLIRHAGV
                     GILVLGSVFLVLTGAEALYADMGHFGRRPIQLGWFVVALPALLLQYFGQGALLMRTPA
                     ALANPFYFLAPDWMLYPLIALATMATVIASQAMLSGAFSLTLQAVQLGYLPPLRITHT
                     SAEQHGQIYFGLLNWFMFIGTVGLILSFGSSSNLAAAYGIAVSGSMIITTLLMYRVVQ
                     AHWQWSPLPAAMAVGGFLLIDLAFFLANAQKIPHGGWFPLLLGAAVFTVMSTWARGRA
                     IVADHLREQFPPLLQFARDVLSQVQCRTAGRAVFLSQHPDITPPALLQNVRHNKSLHE
                     QVYILTVRTEPVPYVPSGGPLEITTIQENLFQVVARCGFMETPDIPRVLTYLAARGHT
                     LPVEETTFFLSRLTFLATPKPGMAIWREKIFVFLSRNTQRASSYFHLPAEQVVEIGLV
                     LEI"
     gene            6174..>6556
                     /locus_tag="PKK81_07590"
     CDS             6174..>6556
                     /locus_tag="PKK81_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNV32776.1"
                     /translation="MTSPTTPTISSAAQQRIVDLIAKELGVTAPQIAAAVTLLDGGAT
                     VPFIARYRKEATNNLDDTHLRTLEERLLYLRELEERRQTILASIDEQGKLTDELRRAV
                     EQAATKQAVEDIYLPYKPKRRTKAQ"
     assembly_gap    6557..6656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6657..8423
                     /locus_tag="PKK81_07595"
     CDS             <6657..8423
                     /locus_tag="PKK81_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNV32777.1"
                     /translation="TVMPGKETAEEEKFRDYYAYSETIRTIPSHRALAMFRGRTLGVL
                     KLDLGLGESLEAIVPHPCAAMIAAHFGIENRGRRADKWLTDVCYWAWRVKVHLHLSTE
                     LLLQVREAAEAEAIKIFGRNLHELLLAAPAGPKAVLGLDPGLRTGCKVAVVDATGKLL
                     DTATIYPHQPRNDWQGALATITQLVLRHGVELISIGNGTASRETDKFAAEVVKLVAEQ
                     KPEQKLAKIVVSEAGASVYSASAFAAAEFPALDVSLRGAVSIARRLQDPLAELVKIDP
                     KSIGVGQYQHDVNQHALARSLDATVEDCVNAVGVDVNTASAPLLARVSGLNRVLAQNL
                     VEYRDTHGRFQNRHMILKVPRLGEKTFEQAAGFLRINDGDNPLDRSAVHPEAYPVVER
                     MLARLNKGIAEVMGKPAALKELSPAEFTDETFGLPTVRDILTELEKPGRDPRPEFKTA
                     TFRDGVESLADLQAGMVLEGVVTNVAAFGAFVDIGVHQDGLVHVSALANKFVKDPHEV
                     VKPGQIVKVKVLAVDVPRQRISLTMRMEDAATPASQPDPRAGGPRDTRDRRPADQQRG
                     GSREPQPIGAFALALARAKEKK"
     gene            complement(8462..9064)
                     /locus_tag="PKK81_07600"
     CDS             complement(8462..9064)
                     /locus_tag="PKK81_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNV32778.1"
                     /translation="MTRPGSSGEQRAQERFGTSARAAAFYAHQTLPHLNPQMQQFIAR
                     MDMVFIATADGKGACDCSFRAGEPGFVQVLDEKTLVYPEYRGNGVLASLGNILENPHI
                     GLIFPDFYQSTVGLHVNGTARVLNPSETESLSQLPARIAEAAHIKGGRHPKAWVLIGV
                     EEAYIHCSKHVPLLKRLDKQIAWGSDDEQAKGGNFFKVTP"
     gene            complement(9177..10535)
                     /locus_tag="PKK81_07605"
     CDS             complement(9177..10535)
                     /locus_tag="PKK81_07605"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV32779.1"
                     /translation="MTMEHMRALIVDDEEFVRLVVEQALREEGCDTQTAGGGQEGLDR
                     LRTASFDCVITDLRMPGLDGRAILRWVKEHQPDVDVIVLTGHGDVKDAVAAMKDGAWD
                     FLIKDTPFDGAAVKAALARLRTVRELRRENLAARHGGYRQDAIVEGTSQAWRTVKTQI
                     AQVAPSQAPVLIQGETGSGKDVVARLLHAHSRRAGGPFIAVNCGAVSRELLESELFGY
                     EKGAFTGAAQAKPGLIAAAEGGSLFLDEIGEMPGPMQVSLLRFLDRKEYRPVGSTRTL
                     RADVRIICATNRDIQELVLQGRFRDDLLYRINTVTLHVPPLRERPEDLAVLADHILHH
                     LRIPGTATRTLSPEALAHLATYRWPGNVRELRNVIERIVLMSPNSGPITSEEVLQVLP
                     RAASASSPDDRSHLPLDEIERLHIQLVLESSGGNKTKAAQTLRVDYKTLLAKLKKYEG
                     GG"
     gene            complement(10540..>12138)
                     /locus_tag="PKK81_07610"
     CDS             complement(10540..>12138)
                     /locus_tag="PKK81_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32780.1"
                     /translation="HEACMRWLHDRSIGQKFFISFGVILSLLALSLTALLVYLSRINS
                     YVDRHKRITVPAIVTAATMQRSAYEMNLTLHLFLEQITKADAADTLARLSRHAEQIRS
                     SLDLYRSTHAARTHPILLGMLTQHQRMDLADQEDRAIARIDLILQELSALWQTALTGP
                     QRAGVAPSPVTKTDGLIVELMTNLDQLVAAHRDIDVEMKVEGDLLLQQARLIALSLVA
                     VLGLVITVIYVSVNRQIAKPLQRLSVTADRVAHHDLTAQFESWPGRDEVGTLAASLSS
                     MVTSLREQTAATARKTKELEAFTYSVAHDLKGPLREIEGFSSLLEKQFADGGDAQTRH
                     QIDVIRRSALRLTHMIDALLKYSRLEQQDLPRQRFNVLEMITTLITDRFNGLQGPKPK
                     IQVALPFADLYGEPVSIRQAIANLLDNAAKFSRHASPPTITIGGTRMEHERILWVQDN
                     GIGFDPSHYDKIFGLFERLHSPQEYEGTGVGLAIVKLVMDKHDGRVWVESAPGAGSKF
                     YLAFPERAESVVDRPSSSPHPASA"
BASE COUNT         2367 a         3358 c         3891 g         2322 t
ORIGIN      
        1 cccggtttga tgttcaatgc cgtgtcttat gatccgcgga aggaaggggt ctatcaggct
       61 gtattccgcc tgctcgacga acttatcgac gccctgcatc cccgggcgat ccatatcggc
      121 cacgacgaag catttggatg gacggtgggc caggtggcga aatggctgaa gttcggtgag
      181 ctcatggtcc cggcggacct ctttgcccgg gatgtcgaac gcattcacgg ctacctgact
      241 caaaaggggg tggagacctg gatgtgggcc gacatgctgc tcgatccggc ggagttcccc
      301 ggcgcctcgc ccaagcatct gcatggggtg gcggcagggt atgggaaagc gcttcgcaat
      361 cggcttccac gggatatcgt gatgtgtgac tggcactacg gcgacgagca ggggagtttt
      421 ccctcgatgg ccgtgatgca gagcgaaggg tttcgcgtga tcggagccac ctggaaacgg
      481 gaggagacca tgcgcaattt cagccgctat gcgttgtccc ggcacgccta tggtctgatg
      541 gcgacaacct ggttccacgt gcagcggaac gaccgggact tggtcgactg gattattcgc
      601 tcgtccggta tgttattccg taaccccgat gcggcggtgc cgccgagacc tgcttcggct
      661 gcccctccgg aaggacatgg ctggacggct ccataacggc ggggaaaggt cgctcttgag
      721 tgggaccgtc agggccccgg cgcgagggga acgcctgccg gttccgctgt gtcatggtga
      781 gccgggctag cggccgatct gtttaggctg ccccgctttg gctagctgca cattcacggt
      841 cacgactccc tcattgagat tacgataggg gtaggcagag gtgataagag ggagggtatc
      901 ggcttgttcc tgtgtggtca cgagggtgag cgccggcaca taccggcctg aagcgacttt
      961 gaccccgatc agcttcgctc ccggttcgac caccacgtgg tcaccgagtt cagtgcgaaa
     1021 cacaagggcc tgcatgccga caaaggtatc gttaccgatt ctggcggggc catgcacctg
     1081 cgattggtgg gccagtgaga cgcgcttgcc gatatagacg gaataggtct tgcccgagac
     1141 ctgcacttcg ttctccggca attcatggtc cccttcgaac gtctccagtc catggatgac
     1201 gaccccgtcc tgcacgttac tgtgatcccc gacataaatg ttctgtcctt catcgccgcg
     1261 gaccgaagcg cccggtgcga tgaaaatcaa ctcgccgata gtcacggatc caatgaccgc
     1321 cgccagggga tgaatgcgtg ccgatgcggc aatcgtcggc acagcgacgt ggggattaaa
     1381 tgtgttggga acgttggggg ctacccagcc gaccagcgtg atgccgatga ggagcaggtt
     1441 caggctcagg ctgagtgaga ggggcaagga tcgaggcatc aagaattctc cgggaaacac
     1501 gggcgctcac ccctgtatgg atgagcctgt cgttgtgccc tgcatcacga tcgagtgaac
     1561 ctcagcgtac aaggcttgct gccactcggc ttgcaggccc ggcgtcatgt cggtgtcgta
     1621 ttctttgacg gtggctcacg ctgttgatcc agtattcgtc gcgctggttc acgcctcttg
     1681 ccgcataggc tcccctcgtc tccatgtcgg cggatggcgc cttaaacttg aggtacgcgt
     1741 cgagtctcct catcccggca ctcgggagtg gaccggaccg gcctggaaca cgtagaatgc
     1801 gcctccgcgg tgctcacccc ctccacccgt gagggcgtgg gaaacgacag ggttgaaaca
     1861 acagtgggct gactaggaag ggatcgtcat gagtgtgaaa tgtggaatcg tcgggctgcc
     1921 gaatgtgggc aagtcgaccc tgttcaatgc gttgaccaag tccgggatcg cagccgagaa
     1981 ctatccgttc tgcacgatcg agccgaatat cggcatcgtg gaggtgccgg atgcgcggat
     2041 gcaggcgctg gcggacatcg tgaagccgca gcggatgcag tatgcgacga ccgaattcgt
     2101 ggacatcgca gggttagtgg ccggagcctc gaaaggggaa ggcctgggca accagttttt
     2161 ggccactatt cgcgagaccg acgggattgt gaatgtcgtg cgctgttttg aagatgacaa
     2221 cgtcgtccac gtggcgggga aggtcgatcc gatttccgac atcgcgacca ttgtgacgga
     2281 actcgcgctg gccgacctca ccacagtgga aaaagctcag gagcggaacg tgaagctcgt
     2341 tcgctcgggc gataaagatg ccgcaaagct aggcgagttg ctggtgcagg tggccgcctg
     2401 tttgaatgag gggaagccgg ctcgcacctt gaaactcgat ccggcgcagc gcgccttgtt
     2461 gaagcccttg tgcctgctga cgatgaagcc tgtgatgtat gtggccaatg tggctgaaaa
     2521 agggtttgcc aacaatccgc tgctgacgcg ggtggaagaa tatgctgcgc atgaaggggc
     2581 gccggtcgtc gccatctgcg ccgcactgga gtccgaaatc gcggtgctct cagacgaaga
     2641 gaagcgggaa ttcctggccg acatcggcat gaccgaaccg gggctgaatc gactgatccg
     2701 cgcggcatac caactcctgg gcttacagac gtattttacc gccggcgtga aagaagtccg
     2761 cgcctggacg attcacatcg gcgatacggc gccgcaggcc gccggcgtca ttcacggcga
     2821 tttcgagaag gggttcattc gcgccgaggt gatcggctac aacgacttca tcgcctgcaa
     2881 aggcgaagcg ggggcgaagg aaaaagggaa gatgcggctg gaagggaagg agtacgtggt
     2941 gcaggacggc gacgtgatgc acttccggtt caacgtctag cggcgctctt ttaccatatt
     3001 acgaggcagg gtttgcttgc ctgatcggag atccaacgtg aacgaacaag acacacagcg
     3061 agctgtagcg attctcaaca gaattatgga acatgagttg gccggcgtag ttcggtacac
     3121 gcactacgcg ctgatggtct acgggtacaa ccgtatcccg attgtgtcgt ggctgaaggg
     3181 caatgcggat gagagtctag cccatgccca caaagccggt gagctggtga ctctattggg
     3241 cggccatcct tcgttgaaga tcggtaccct gctcgaaacg gagaagcatg atgtgggaga
     3301 tattctgcgg gagagtctgg agcacgagaa agccgccgtt gcctcatatt acgaattgct
     3361 gaaactgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ggggaacatc
     3481 atccgtctct gcgccaacac cggtgcctcg ttgcatctgg tcaagccgtt gggctttacg
     3541 ctggaagaca aacagttgct gcgagccgga ctggattatc acgaattcgc ctcgatcacg
     3601 gtgcacgagg ggtgggccga gtgcctagca cgcatggaag ggcgccgaat tttcgccgta
     3661 tccacgaagg gcagacagcg gtacgatctg gttcgttaca tcgaaggcga tgcgtttgtc
     3721 tttggcccgg aaagccgtgg attgccggcg gagattcttg gcagcgtccc tgaacagcag
     3781 tgcctgcgcg taccgatggt gccgggaagc cgcagcctca acctctccaa ctcggtcgcg
     3841 gtggtgctct acgaagcctg gcggcaggtc gaattttgga aaggtgtctg atcgccggtc
     3901 attccttcgc aggcaccgcc ggatcccacc gggcagggct cgtcacagac cgctcagcga
     3961 cgcgatcaaa tctccagcac cagtccgatt tcaaccacct gttccgccgg taggtggaaa
     4021 taggaactgg ctctctgggt atttcgtgac aggaagacaa atattttttc ccgccagatt
     4081 gccatccccg gtttcggcgt cgccaaaaag gtcagtctgc tcaggaagaa cgtggtctcc
     4141 tcgacaggga gggtatggcc gcgggctgcg aggtaggtga gtacacgtgg aatgtccggc
     4201 gtttccatga atccgcatct ggcgacaact tgaaacaggt tttcctggat ggtggtgatt
     4261 tccagcgggc ctccggatgg aacgtagggc acaggctctg tgcggactgt gaggatatag
     4321 acctgctcgt gcagagattt gttgtgccgg acattttgca gcaaggcggg cggggtgatg
     4381 tcgggatgtt gagataaaaa tacggcgcgc ccggcggttc tgcattgcac ctgcgacaag
     4441 acgtcccggg cgaattgaag caatggagga aactgctcgc gcaagtggtc tgccacgatc
     4501 gcgcgtcctc gcgcccaggt actcatgacg gtgaacacgg ccgcgccgag cagcagcggg
     4561 aaccatccgc catggggaat tttttgggca ttcgcgagaa agaaggcgag atcgatgagc
     4621 aggaagcccc cgactgccat ggctgccggt agcgggctcc attgccaatg ggcctggacc
     4681 acgcggtaca tcaggagggt cgtgatgatc atggaacccg agacggcgat gccataggct
     4741 gcggccagat tactggacga tccgaaggaa aggatcagac cgacggtgcc gatgaacatg
     4801 aaccaattca acaggccaaa gtagatttgg ccatgttgct cggcggatgt gtgggtgatc
     4861 cgcaacggcg gcagatagcc caattgaacg gcttgcaagg tcaacgagaa ggccccggac
     4921 agcatggctt gggatgcgat gacggtggcc atggtcgcca gcgcgatcaa ggggtacagc
     4981 atccagtcgg gggccaggaa gtaaaaggga ttggccagag ccgccggggt ccgcatgagg
     5041 agcgcaccct ggccgaagta ctggagcaga agggcgggca aggccaccac aaaccagccg
     5101 agttggatcg gtcggcgtcc gaagtggccc atgtcggcat acagcgcctc ggcacctgtg
     5161 aggaccagaa acacactgcc cagcaccagg atgccgactc ctgcatgccg aatcaaaaac
     5221 tggatcgcat acagcggatt ggcggcacgc agaacctccg gggtttggat caaactgtgg
     5281 agcccgagga gggccatcgt gacaaaccag agcagcatga tggggccgaa gatgctcccg
     5341 atcgttccgg agccccggcg ttggatcatg aacaacaagg cgaccagcgc gacggtcaga
     5401 ggcaggatgt agggggtaaa cacatcggtg gtcagcgtga gcccttcgac cgcgctaagg
     5461 accgaaatgg ccggtgtaat gatgccgtcg ctatagagaa acgccacgcc gatcaagccg
     5521 agtgtgacga caacgttgag ccctttgcgg agatcgaccg ggcgggactg ctggctgagc
     5581 gccaggagtg ccagcatgcc tccttcgccc tcgttgtcgg ctttcatgac gaacagcaga
     5641 tatttcacgg tgacgacgag cacgagcgac cacacgatca tggacagaat ccccagcacg
     5701 ttgtcgggag tgaccggcag gccatgcgat tcgtgaaaac actcgcgcag ggcatacagc
     5761 gggctggttc cgatgtcgcc atagaccact cccatggcgg ccacggtgag ggagacggaa
     5821 gaagattggt tgttgagatg cttcggcatg gatacgtgac aggggcccga cccgattcgc
     5881 gatggtcctg tgttgtgacg ggtcgaacta taccatgttg gatgagagga tgccggttgg
     5941 ttccgcaggg cgtcgtaagc cgccgcatct gctcccatgt ggagagaggt gagtggccgc
     6001 gcatccggcc tggaagttgc gcgaatcttc tgctagattc cgagtcggct cgtgagaacg
     6061 ttcataggcg tcgcgccggc cgagtccttc accttcattt gacaagaaag ccactgagac
     6121 cgggggagta gcctcccgcc gggtctcgct ttcggaaagc ccaatacgca cctatgactt
     6181 ctccaacgac accgaccatt tcatccgcag cccagcaacg catcgtcgat ctcatcgcca
     6241 aggaacttgg cgtgaccgcc ccgcagattg cggccgcggt gacgttgctg gacggtggag
     6301 cgacggtgcc ctttatagcg cgttatcgca aagaggccac caacaacttg gacgacacgc
     6361 acctgcgcac cctggaagag cgcctcttgt atctccgtga attggaagag cggcggcaga
     6421 cgattctcgc gtcgatcgac gagcagggga aattgaccga cgagctgcgc cgggcggtcg
     6481 agcaggctgc gacgaaacaa gccgtggaag atatctatct gccctataaa cccaaacgtc
     6541 gcaccaaggc gcagatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgg
     6661 tcatgcccgg caaggaaacg gcggaagaag aaaagtttcg cgattactac gcctattcag
     6721 aaaccatccg taccattccc tcgcatcgtg ccctggcgat gtttcgcggc cgcacgttgg
     6781 gtgtgttgaa gctcgacctc gggttagggg aaagtctcga agccatcgtg ccgcatccct
     6841 gcgcggcgat gattgccgcc cacttcggta ttgagaatcg cgggcgccgt gccgacaaat
     6901 ggctgaccga tgtctgctat tgggcctggc gcgtgaaagt gcatctgcat ctgagcaccg
     6961 agctgttgct gcaggtgcgc gaagccgccg aagcggaggc aatcaagatc ttcggtcgta
     7021 atctccatga gctgctgctg gcggcgcccg ccggtccgaa ggccgtgctc ggtctcgatc
     7081 ctggtctccg caccggctgt aaagtggcgg ttgtggatgc gaccgggaaa ttgctggaca
     7141 cggcgacgat ctacccgcat caaccgcgta acgattggca gggggccttg gcgacgatca
     7201 cccagttggt gcttcggcac ggtgtcgaat tgatttccat cggcaatggt accgcaagcc
     7261 gggagaccga caaatttgcc gccgaagtcg tcaagctggt ggcggagcag aaaccggaac
     7321 agaaactggc gaagattgtg gtaagtgaag ccggcgcctc cgtctattcg gcctcggcct
     7381 ttgccgccgc ggagtttcca gcgctggatg tgagtctacg cggcgccgtg tcgattgcca
     7441 gacggttgca ggatccgttg gccgaactgg tcaagatcga tcccaaatca atcggcgtcg
     7501 gccagtacca acacgatgtc aatcagcatg ccctggcgcg atcgctcgat gcgacggtcg
     7561 aagactgcgt gaatgcggtg ggtgtggacg tgaataccgc gtcggctccg ttgctggcga
     7621 gggtatccgg cttgaatcgc gtgctggcgc aaaacctcgt ggagtaccgt gacacccacg
     7681 gtcggttcca gaaccggcac atgatcctca aggtaccgcg cctcggcgaa aaaacattcg
     7741 agcaggcggc cggcttcttg cgcatcaacg acggcgacaa cccgttggat cgttccgccg
     7801 tccacccgga agcctacccg gtggtcgagc gcatgctggc gcggttgaat aaaggcattg
     7861 ccgaggtcat gggcaaaccg gccgcgttga aggaactctc gccggcggaa ttcaccgacg
     7921 agaccttcgg tctgccgacg gtgcgcgata tcctcacgga gctggaaaaa ccaggccgtg
     7981 atccgcgtcc ggagttcaag acggccacct tccgggacgg ggttgagtcg cttgccgatc
     8041 tgcaagccgg gatggtgctc gaaggagtgg tgaccaatgt cgcggccttc ggcgcattcg
     8101 tcgatatcgg cgtgcatcag gacggcctcg tgcatgtgtc tgcgctggcg aacaagttcg
     8161 tcaaagatcc gcatgaagtc gtgaaaccgg gccagatcgt gaaggtgaag gtgctggccg
     8221 tcgatgtacc gcgacagcgg atttcgctga ccatgcgcat ggaggatgcc gcgacgccgg
     8281 cctcacagcc tgatccccgt gccggagggc cgcgtgacac gcgcgaccga cgccccgccg
     8341 atcagcagcg gggtggatcc cgtgagcctc agcccatcgg ggcatttgca ctggccctgg
     8401 cccgcgccaa ggagaaaaag tagcgagcgg cctgctcgcc tccggcgcgc tggtgagtgg
     8461 ctcagggagt gaccttgaag aaattcccgc ctttggcctg ctcgtcgtcg ctgccccaag
     8521 caatctgctt atccagtcgc ttgaggagcg gcacatgctt ggaacagtgg atgtaggctt
     8581 cttccacgcc gatgaggacc cacgccttgg ggtggcgccc gcccttgatg tgagcggctt
     8641 cggcgatgcg cgccggaagc tgggagaggc tctccgtttc gctcgggttg agcacacgcg
     8701 cggttccatt gacgtggagc ccgaccgtgc tctgatagaa gtcgggaaaa atcaacccga
     8761 tgtgcggatt ctccagaatg ttccccagac tcgccagcac cccgttaccc cggtattccg
     8821 ggtagaccag cgtcttctcg tcgagcacct gcacgaatcc cggttcgccg gctcgaaagg
     8881 aacaatcaca cgcacccttc ccgtcggcgg tggcaatgaa gaccatgtcc atccgtgcga
     8941 tgaactgttg catctgcgga ttcaggtggg gcagggtttg gtgggcataa aatgctgccg
     9001 cgcgagccga ggtgccgaag cgctcttgtg cgcgctgttc gcctgatgat cccggcctgg
     9061 tcatatcgaa cgctccttgt ggaagatgtc taagacggtg ctgcagaggt gattgtccct
     9121 catgcacgct ggcgctgtcg atgagtgttg tcgtggagaa gtgtacaaag acccgcttag
     9181 cccccgcctt cgtacttctt cagttttgcc aacagcgttt tgtagtcgac ccggagggtt
     9241 tgggcggctt tggttttgtt gccgccgctg gattccagca ccagttgaat atgcaggcgt
     9301 tcgatctcgt cgaggggcag gtgcgaccgg tcgtcgggtg atgatgccga tgccgcccga
     9361 gggagcacct gcaggacttc ttcgctggtg atcggtccgg agttcggact catcaggaca
     9421 atgcgttcga tcacattgcg aagttcgcgc acgtttccag gccagcggta ggtcgccagg
     9481 tgtgcgaggg cttccgggct gagcgttctc gttgcggtgc ccgggatgcg aaggtggtgc
     9541 agaatatgat cggccagcac ggcaagatcc tccggtcgtt cgcgcaacgg cggcacatgc
     9601 agcgtcaccg tgttgatacg atacaggaga tcgtctcgaa accggccttg cagcacgagt
     9661 tcctggatgt cccgattcgt ggcgcagatg atccggacat cggcccggag tgtgcgcgta
     9721 ctgccgacag gacggtattc cttccggtcg agaaagcgca gcaggctgac ctgcatcggt
     9781 cccggcattt cgccgatctc gtcgagaaac aacgagccgc cttcggcggc ggcgatcagt
     9841 ccgggtttcg cctgggcggc gccggtaaac gcccctttct cgtaaccgaa taactcgctc
     9901 tccagcaact ctcgactcac cgctccgcaa ttgacggcga tgaagggccc gccggcccga
     9961 cggctgtgcg cgtgcagcag ccgcgcgacc acgtctttcc cggaaccggt ttccccctga
    10021 atcagcaccg gggcctgcga cggcgccact tgcgcgatct gtgtcttcac cgtccgccag
    10081 gcctggctgg tcccctcgac gatcgcgtcc tggcgatacc cgccgtgccg cgccgccaga
    10141 ttctcgcggc gaagttcccg caccgtgcga agtcttgcga gagcggcctt cacggccgcg
    10201 ccgtcgaacg gggtgtcctt gatgaggaaa tcccacgcgc cgtctttcat cgcagccacg
    10261 gcgtctttga cgtcgccatg gccggtcagg acgatcacat cgacgtccgg ttgatgctcc
    10321 ttcacccagc gcaagatcgc ccggccgtcg agaccgggca tgcggagatc ggtgatgacg
    10381 cagtcgaagg aggccgtacg gagtcgatcg aggccttcct gcccgccacc cgccgtctgc
    10441 gtgtcgcagc cttcctcgcg gagcgcctgt tccacgacga gacggacaaa ttcttcgtcg
    10501 tcgacaatga gggcgcgcat gtgttccatg gtcatgaggt cacgctgaag caggatgagg
    10561 tgaagaggat ggccggtcga cgacggattc ggcgcgctcc ggaaaggcca gatagaattt
    10621 actgcccgct ccgggagcgg attccaccca gacccggccg tcatgtttgt ccatgacgag
    10681 tttcacgatc gcgaggccca cgcccgtgcc ctcgtattcc tgtgggctgt ggagccgctc
    10741 gaagagcccg aagatcttgt cgtagtgaga cgggtcgaag ccgatcccgt tatcctgaac
    10801 ccacaggatg cgttcgtgtt ccatccgggt gccgccgatg gtgatggtcg gcggtgaggc
    10861 gtggcgggag aacttcgctg cattgtcgag gaggttcgcg atcgcttgac ggatgctcac
    10921 cggttcaccg tagagatcgg caaacggcaa ggcgacctgg attttcggct tcgggccctg
    10981 gagtccgttg aaccggtcgg tgatgagggt ggtgatcatt tccaacacat tgaagcgctg
    11041 acgaggcagg tcctgttgct ccagccggga atatttgagc agcgcgtcga tcatatgggt
    11101 gaggcgcagc gcggagcggc gaatcacatc gatctggtgc cgggtctgcg cgtcgccgcc
    11161 gtcggcgaat tgtttttcca gcagggagga gaagccttcg atctccctca acggaccttt
    11221 gaggtcatgc gcgacggaat aggtaaacgc ttcaagctct ttggtcttcc gggcggtcgc
    11281 ggcggtctgt tcgcgcaggc tggtgaccat ggatgagagg gaggcggcca aggtgccgac
    11341 ttcgtcacgg ccgggccagg attcgaattg cgcggtcagg tcgtggtgag cgacacggtc
    11401 ggccgtgacg gagagacgtt ggagcggctt ggcgatctgc cgattgacgg agacatagat
    11461 gacggtgatc accagaccga gcacggcgac caggcttaac gcaatcagcc gcgcctgttg
    11521 gagcaacaga tcgccttcca ctttcatttc cacatcgata tcccgatggg cggcgaccag
    11581 ttggtcgaga ttggtcatca gttccacgat gaggccgtcg gtcttcgtta ccggtgaggg
    11641 tgccacgccg gcgcgctgag gtccggtgag cgcggtctgc cacaaggcgg acagctcctg
    11701 gaggatcaga tcgatccggg cgatggcgcg atcttcctgg tcggcgagat ccatgcgttg
    11761 atgctgggtc agcatgccca ggagaatcgg gtgggtgcgg gccgcatggg tggaacgata
    11821 caggtcgagg gaactgcgga tctgttcggc gtggcggctg agccgggcaa gggtgtcggc
    11881 cgcatcggcc ttcgtgatct gctccagaaa cagatgcagc gtgaggttca tttcataggc
    11941 tgaacgttgc atggtggccg cggtcacgat ggcggggacc gtaatgcgtt tgtggcggtc
    12001 cacatagctg ttgatgcgac tgaggtacac gagcagcgct gtgaggctca aggccagcag
    12061 gctgaggatg actccgaagg agatgaagaa tttttgtccg atcgagcggt cgtggagcca
    12121 gcgcatgcag gcctcgtg
//
LOCUS       DAOQRM010000075        11851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_4550_length_11851_cov_19.166243, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000075 DAOQRM010000000
VERSION     DAOQRM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4550_length_11851_cov_19.166243"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKK81_07615"
     CDS             <1..500
                     /locus_tag="PKK81_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNV32781.1"
                     /translation="ALGGSNHFFKYYADVIKYTPLIYDTRFSVHVRYGSTEGIGGRPI
                     PLTERFFVGGINTMRGFVFGRAGPVTPNGSLLGSAKELIFNNDFIFTISSEAKLNGVF
                     FFDYGNGFDDNEPVQFNKLRSAAGFEARWISPFGPLRAAYGINLDPRPTERMGVFEFT
                     IGSLF"
     gene            539..1114
                     /locus_tag="PKK81_07620"
     CDS             539..1114
                     /locus_tag="PKK81_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNV32782.1"
                     /translation="MGGPVGRSGSRGAGRAGLCLALVTVALVVGGCRSGHSTVPTSTR
                     IGVVDPQRVLSDTNAGRKAKDMLASFAKNRQALIELEEKELRRMEEDFMKQGSVLSAN
                     AKREREEQFRRRMSEYQQKVTDLNREVQDKQKEVLDGFREKIETLSGRVAKRLELQAV
                     FDRGRGGPTLYFDEAVDVSSQVIEEFNKTYP"
     gene            1126..1662
                     /locus_tag="PKK81_07625"
     CDS             1126..1662
                     /locus_tag="PKK81_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNV32783.1"
                     /translation="MNVRTWIVAGAVPVVLAWLAQPVQAADRIKVAMMDQQQVVERSV
                     AGKRALEDLKSYSTTRQKIIDSDDQELKELEKALQDSALTEEVRKEKQEQFRAKLEAY
                     QRRIQDFNREVQEKQKGMVLDFAQRIHAAVSAVAQKEGYTAVIDRGSESTVKIVVYGH
                     PSVDLTEQIVKEFDRQNK"
     gene            1705..2160
                     /gene="fabZ"
                     /locus_tag="PKK81_07630"
     CDS             1705..2160
                     /gene="fabZ"
                     /locus_tag="PKK81_07630"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNV32784.1"
                     /translation="MSVMDNVEIQSILPHRYPFLLVDRIRELDPDRRIVGIKNVTINE
                     PFFQGHFPGRPVMPGVLILEALAQVGGVLAFKSLGSVGRPVVYLTGIDGAKFRKPVVP
                     GDILRLEVDVLKKRAPFWKMQGRAFVESELVCEAEVTAMVTDEQAGGDK"
     gene            2229..3038
                     /gene="lpxA"
                     /locus_tag="PKK81_07635"
     CDS             2229..3038
                     /gene="lpxA"
                     /locus_tag="PKK81_07635"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNV32785.1"
                     /translation="MNIHPTAVVHPKAVLADDVEVGAYSVVGEHVRIGAGTRVLSHVC
                     IDGWTDIGERCELHPFVSVGGPPQHMQYKGEPTKVVIGHDNILREYVTVNRATVQGGG
                     VTSIGDANFLMAYVHVAHDCHLGNHLILANAASLAGHITIGDHAIIGGLSGIHQFVRI
                     GAYAMVGGCCALGQDLPPFMRAAGGYRARMYGLNSIGLRRHGFSSERIAALKKSYEVL
                     FRSGHRVAEAVKLARENFSASPDVMQVAAFMEGTKRGICRSVGKDQEGEEE"
     gene            3042..3884
                     /gene="lpxI"
                     /locus_tag="PKK81_07640"
     CDS             3042..3884
                     /gene="lpxI"
                     /locus_tag="PKK81_07640"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNV32786.1"
                     /translation="MGKQVAEHRQASDGQRIGLIAGNGRFPIIFADNAKRLGYSVSAV
                     AHEGETDPELARHVDHIHWIKIGQFGKLIEALKGDGVQRAVMLGGIKKTHVFSTVRPD
                     FRALALAAKLIHLKDDDILRRVAEEIEQEGIQICESTFGLEGILVEEGTLTRREPSKK
                     EWEDIRYGWDVGKQIGALDIGQCVVVKDRVIVAVEAVEGTDGAIRRGGELAHGGAVVV
                     KRCKPQQDLRFDLPAVGPRTIEVMESVKASVLALEAGRGILLDRDETIAKANRAGIAI
                     IGMT"
     assembly_gap    4177..4276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4277..5285
                     /locus_tag="PKK81_07645"
     CDS             <4277..5285
                     /locus_tag="PKK81_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV32787.1"
                     /translation="ETGLGAMKQLRAGVIGVGHLGQHHARHYATLPGATLVGVCDASP
                     TRAKLIADRHGVQFWTDLDDLLKQVDIVSVAAPTSAHFSATKACLEAGKHVLVEKPIA
                     VTSAEARELVELAARRGCLLQVGHSERFNPIMQRMRPFIERPAFIECHRLSAFGERGT
                     DVDVVLDLMIHDLDLVLSFNPGPVEEVRAAGVPVLSSNIDIANARIAFASGCVANVTA
                     SRVSTNKMRRLRVFQRDRYVSIDFQTRQAVVSRRVQAAGAKPTIDIESYQAGDEEPLR
                     LELDSFIHAVNTGTRPVVSGEDGEAALNLATLVLEAIGRFTQRHSADEAAAVAFGREN
                     V"
     gene            5325..6458
                     /gene="lpxB"
                     /locus_tag="PKK81_07650"
     CDS             5325..6458
                     /gene="lpxB"
                     /locus_tag="PKK81_07650"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNV32788.1"
                     /translation="MPRILIVTGEASGDLHGAHLVKALKELSPALQIVGVGGASMLAA
                     GAELVKDIPQLDVMGLIGLSAVKTMLQRISRIRTLIKGERWDLVVLIDNPGLNFHFAR
                     VARACGLKVLYYIAPQVWAWRRGRMRWIQQRVDHVLAILPFEEPLYKKAGVRCTFVGN
                     PLLDEVAPSYDRQALRRQFGLSDAGPVIGLFPGSRKGELLEHIPLLLETVKRLAQRHP
                     AIQFILAQASSIQDQFLADLLKDSPVPIRVFRNQASEVMAASDLLVVKSGTSTLQAAV
                     VGTPMILFYRASSWLTYRLARLLIRVPWIGLANLVAGRGIVPELIHDEATPERLIQET
                     ERLLGDPRAYEEMKAALLTVRQALGTPGASRRAAEAVLAECRA"
     gene            6455..8191
                     /gene="msbA"
                     /locus_tag="PKK81_07655"
     CDS             6455..8191
                     /gene="msbA"
                     /locus_tag="PKK81_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNV32789.1"
                     /translation="MKHYLRLLKYLNPYRFRLGAAFLCALLVAGLSAAYAWLVRPVLD
                     GLFISKDESLLLVLPIAILAVAVLKGVFNYGQNYLMNYVGNQVIGDIREQLFSKLVRL
                     PVHFHDTNTSGRLVSRVINDVNQMANAVAGVLKDLFQQGLTFLAMIGVIIYQNWKLAA
                     VSMIVVPLSVVTMARMGKRLRNLATRGQERMGDMASTLQETLAGIRMVKSFGREEEEA
                     KRFRLSNDAFIHTTMKAIQVSSLGSSHMEVIGVLGVAGIIWYGGYLVIHDEMTPGAFF
                     SFLAAMFMAYTPIRRLSGANNTVQQALAAAERVFDVLDLPTEPEANGGQTDLPAISRS
                     LEFRQVAFQYEGQGEPALSGIDLTIRAGEIIAFVGSSGSGKTTLVSLLPRFYDPTGGQ
                     ILIDGVDLQTCSLRSVRGQIGIVSQEVVLFDDTVRNNIGYGRQGATPDDVMRAAQLAY
                     AHEFIVRMPAGYDTLIGERGLKLSGGERQRLAIARAILRDPPILILDEATSALDTQSE
                     RIVQLALTNLMHNRTTLVVAHRLSTIQNANRIVVLDRGRVAEVGSHEELLRKGGMYKR
                     LHAMQFADAISE"
     gene            8224..8886
                     /locus_tag="PKK81_07660"
     CDS             8224..8886
                     /locus_tag="PKK81_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV32790.1"
                     /translation="MTTDRSAAAPQPQPLLKRVVNALKVAVVPPVGYQIIQMLRRTMT
                     WRTEGSEHVNRLFAEEKRVILAFWHAQQLMMPLAIPGLEAHVLISQHRDGELIRRIVA
                     RFGLDAVRGSSTRGGAEAFRQLIRLGRSGGNLVLTPDGPKGPRQVVKVGVVQLARATG
                     LPIIPMAFGCSKKNSSRAGTGSSCPIRSRGASFSWGRRSACRPTPRPTTLSVSAGSWK
                     TR"
     gene            8936..10291
                     /locus_tag="PKK81_07665"
     CDS             8936..10291
                     /locus_tag="PKK81_07665"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNV32791.1"
                     /translation="MWYLLYNSLLLLVSPIILFVLLAKQRCRRGLPQRLGLRAEMPTT
                     DPAGRSGCIWIHAVSLGEVVAVAPLVRDLRRRYPETRLVVSTVTETGREAVEQRLEGV
                     AEHRYAPLDFPWVVNQAIDRLKPSLYIFVETELWPNILRSLRRRNVPSILVNGRLSTR
                     SFERQRLPVIRDFYRTMLNMISCCLMQSERDAQRMIDLGAEPSRVRCTGNIKFDQPVP
                     QAAAGGTAVSKAALGLTDRELLLVAGSTHPGEEETIVNAYQILSPEFPELRLVLAPRH
                     IERAAQVEQMIQAKGLTVTRRSTGGQVPMASTGARVLVLDTRGELALLYRDAVVAFVG
                     GTLAPVGGHNLLEPAVWGKPVLFGPHTDHCAEVAALLLNAQGGLVVQDEQDLAQGLRA
                     LLRDPAALLRMGQAAQQVVADNQGALQRSAEIIATFLPARGTSKNARVEQGRELSSRQ
                     S"
     gene            10303..11373
                     /gene="lpxK"
                     /locus_tag="PKK81_07670"
     CDS             10303..11373
                     /gene="lpxK"
                     /locus_tag="PKK81_07670"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNV32792.1"
                     /translation="MSRGLRSWLRWVGYPYELAARLRLWGYTHGWFSTRRLPRPVISV
                     GNLTVGGTGKTPVVMYLVERLTAQGKRVAILSRGYRRRSNAPQLLVSDGQQVLVGPDE
                     AGDEPYLIARRCPQAVVAVGANRYALGQWVLGRLPVDCFVLDDGFQHVQLHRDLNLLL
                     VDATDAAGIQAALPVGRLREPLSAAARASAILITRVDEAHGGESVRCLLLDACGSLPS
                     LVRVGFRAEEFRRVGTGERLPLDAFRGRSAVLFSGIGNAESFRALVAGLGIAVIEMLA
                     FPDHVHYTRGMIDTIRAKAKASGADLLVTTEKDADKVAPLVVPEDACWAVRLRTEIVS
                     GQEDLERLLRLDSAPGRTGGHA"
     gene            11366..>11851
                     /locus_tag="PKK81_07675"
     CDS             11366..>11851
                     /locus_tag="PKK81_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV32793.1"
                     /translation="MRKEGIRRIVVRGPNWLGDAVMCEPALSQVRTLFPQAEITLLVK
                     PGIADLLAQHPEVNRTLVYDDRGRHAGLVGKWTLAAVLRRHRFDLAILFQNAFEAALI
                     SFLAGIPRRFGYATDGRTLLLTDPVTVPPRTAQRHQVEYYWDLLKPLGGHGPAPAPRL
                     FV"
BASE COUNT         2151 a         3265 c         3985 g         2350 t
ORIGIN      
        1 ctgcgctggg cggcagcaat cactttttca agtattatgc ggacgtgatc aagtatacgc
       61 cgctgatcta cgatacgcgg ttctcggtgc acgttcggta cggctcgacg gaggggatcg
      121 ggggccggcc cattcccctc accgagcggt tcttcgtcgg cggtatcaac accatgcgcg
      181 gattcgtgtt cgggcgggcg gggcctgtga cgccgaacgg atccctcttg ggttccgcga
      241 aggaactcat tttcaacaac gattttatct tcaccatttc gtccgaagcg aagctgaacg
      301 gcgtgttctt cttcgattac ggaaatggat tcgacgacaa cgaaccggtc cagttcaaca
      361 aactgcggag cgcagccggg ttcgaagcca ggtggatttc tccgttcggc cccttgcggg
      421 cggcctacgg aatcaacctg gatcccaggc cgacagagcg aatgggtgtg ttcgagttta
      481 cgatcgggtc gttgttctaa ggcagtccgt gtcggtgcgt gagaaaggaa tcgccggaat
      541 gggtggacca gtgggccgca gtggatcgag gggagccggt cgggccgggt tgtgcctggc
      601 gcttgtgacc gtggcgttgg ttgtcggcgg gtgccggtcc ggtcactcga cggtgccgac
      661 ttcgacacgt atcggtgtgg tcgaccccca gcgcgtgttg agcgacacca atgccgggag
      721 gaaggcaaag gacatgctgg cgtcttttgc caagaaccgg caggccttga tcgagttgga
      781 ggaaaaagag ttgcggcgga tggaagaaga cttcatgaag cagggcagcg tgttgagcgc
      841 caacgccaag cgcgagcggg aggaacaatt ccgtcgtcgc atgtcggagt atcagcagaa
      901 ggtcaccgac ttgaatcgtg aagtgcagga taagcagaaa gaggtgttgg acggattccg
      961 tgaaaaaatt gaaaccttgt cgggccgagt ggccaaacgg ttggaactcc aggccgtgtt
     1021 cgatcgcggg cgcggcggac ccactctgta cttcgacgag gcggtggatg tgtcgtcgca
     1081 ggtgatcgaa gagttcaata aaacgtatcc atgatgaggg agcccatgaa cgtacgtact
     1141 tggatcgtag cgggtgcggt gccggtggtg ttggcgtggt tggcgcagcc cgtgcaggca
     1201 gcggaccgca tcaaggtcgc gatgatggac cagcaacagg tcgtcgagcg gagcgtggcc
     1261 gggaaacgcg cccttgaaga tctcaaaagt tattccacga cgcgccagaa gatcatcgat
     1321 tccgacgacc aggaattgaa ggagcttgag aaggcgctcc aggacagcgc gctcaccgaa
     1381 gaggtccgca aggagaaaca ggagcaattc agggcgaagc tggaggccta tcagcgccgg
     1441 attcaagact tcaaccgtga ggtgcaggag aagcaaaagg gcatggtcct ggattttgcg
     1501 cagcgcatcc atgccgcggt atccgccgtg gcccagaagg aagggtatac cgccgtcatc
     1561 gaccggggca gcgagtccac cgtcaagatc gtcgtctacg gccacccgtc ggtcgacctg
     1621 acggagcaga tcgtcaagga gttcgaccgg caaaacaaat agccgggatg tgtaatcgtc
     1681 tacgcaacac aggaggcggg gctgatgtca gtgatggaca atgttgagat tcaatcgatc
     1741 cttccccacc ggtatccgtt tttgctggtc gatcgtattc gcgaactcga tccggaccgc
     1801 cggatcgtcg gcattaagaa tgtgacgatc aacgagccgt tttttcaggg gcatttcccc
     1861 ggccgtccgg tcatgcccgg ggtgttgatt cttgaggcgc tggcccaggt gggcggggtg
     1921 ctggcgttca agtcgttggg gtcggtcggc cggccggtcg tgtatttgac cggcatcgac
     1981 ggggccaaat tcaggaagcc ggtggtcccg ggtgacattc tccgcttgga agtggatgtg
     2041 ctgaagaagc gcgcgccgtt ctggaagatg caaggcaggg ccttcgtcga gtccgaactg
     2101 gtgtgtgaag cggaagtcac ggccatggtc acggatgaac aggccggcgg ggacaagtag
     2161 ccggggctct gtgtcggccg gtggcgtggc gttgaacagc atagcagtcg atggtgagga
     2221 ggttttgagt gaacatacat ccgaccgcgg tggtccatcc caaagcggtg ctcgcagacg
     2281 atgtggaagt gggcgcctat tccgtcgtcg gtgaacacgt ccggatcggc gcaggcactc
     2341 gcgtattgtc ccacgtgtgc atcgatgggt ggaccgacat cggagagcgg tgcgaattgc
     2401 atccctttgt atcggtcggc ggaccgcccc aacatatgca gtataagggc gaaccgacca
     2461 aggtcgtgat cggtcacgac aatatcctgc gggagtacgt gacggtcaac cgggcgacgg
     2521 tgcaaggcgg tggagtcacc agtatcggcg acgccaactt cctgatggcc tacgtccatg
     2581 tcgcgcacga ttgccacctc ggcaatcatc tcattcttgc gaatgcagca agtctggccg
     2641 gccatattac gatcggcgat cacgccatca tcggcggact ttcgggcatc caccagttcg
     2701 tgcggatcgg cgcctatgcg atggtcggcg gttgctgcgc actcggccag gacctgccgc
     2761 cgttcatgcg ggccgccggc gggtatcgcg cccgtatgta cgggctgaac tccatcggtc
     2821 tgcgacggca tgggttctcc tccgagcgca tcgcggcgct gaagaagtcc tacgaagttc
     2881 tgtttcgttc cgggcaccgg gtggcggagg ctgtcaaact ggcgcgtgag aacttcagtg
     2941 ccagcccgga tgtcatgcag gtggccgcgt ttatggaagg gaccaaacgg gggatttgcc
     3001 ggtcggtcgg taaggatcaa gagggcgaag aggagtagcg ggtggggaag caggtggccg
     3061 agcataggca ggcatccgat ggacagcgga tcggactgat tgcgggtaac ggccggttcc
     3121 cgatcatttt tgccgacaat gcgaagcggc tcggatattc cgtgtccgcg gtggcgcatg
     3181 agggcgaaac cgacccggaa ctcgcgcggc acgttgatca cattcactgg atcaagatcg
     3241 gccagttcgg caaattgatc gaggcgctca agggcgacgg cgtgcagcgc gcagtcatgc
     3301 tgggcggtat caagaagacg cacgtatttt ccacggtccg gccggacttt cgcgccttgg
     3361 ccctggccgc taaactgatt catctcaagg acgacgacat cctgcgccgg gtcgccgagg
     3421 agatcgaaca ggaaggtatt cagatctgtg aatccacctt tgggctggaa gggatccttg
     3481 tcgaggaagg cacgttgaca aggcgcgagc cgagcaaaaa ggaatgggaa gacatccggt
     3541 acggctggga tgtcggaaaa cagatcggag cgttggatat cggccagtgc gtcgtggtga
     3601 aggatcgggt gattgtggcc gtcgaagccg tggaaggaac cgatggcgcg atccgccggg
     3661 gcggtgaact ggcgcatggc ggcgccgtgg tggtgaaacg gtgcaaaccc cagcaggatc
     3721 tccggttcga tcttcctgcc gtcggcccgc gcaccatcga ggtgatggag tccgtgaagg
     3781 cgtcggtgct ggcgttggaa gcgggacgcg ggattctcct ggaccgtgac gaaacgatcg
     3841 cgaaggcgaa ccgtgccggc atcgcgatta tcggaatgac gtaacaggct gcggaaaaac
     3901 tcgattcgtg cacctacgca gcgatcagcg tctgtaaggt gtcggcattg aatataatac
     3961 acaggttgcg aaaaaaggcc gtccggcaag gccgcagcaa gcgacgaggt gaatcgtact
     4021 cgggctgtac ggtgagcccc ggcgcgatgc gagaacgccg ctggcggact ttttccgcat
     4081 cctgctcatg tcgttcggca ttggaacgga ggcctgcctc gtgagacagg attgggcgcg
     4141 atgaaacagc ttcgagccgg cgtgatcggt gtcggtnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnntgag acaggattgg gcgcgatgaa acagcttcga gccggcgtga
     4321 tcggtgtcgg tcacctgggg cagcaccatg cccgccacta cgcgactctt cccggcgcaa
     4381 cgttggtcgg tgtgtgcgat gcctcgccga cccgtgccaa gttgatcgcc gatcggcatg
     4441 gcgtccagtt ctggacggac ctcgacgacc tcctcaagca ggtcgatatc gtcagtgtcg
     4501 ccgctcctac ctctgcgcat ttttcggcga ccaaggcctg cctggaagcc ggcaagcatg
     4561 tgctggtcga aaaaccgatc gccgtgacgt cggcggaagc ccgtgaactg gtcgaactgg
     4621 ctgcgcgccg tgggtgcctg ttacaggtcg gacatagcga gcggttcaat ccgatcatgc
     4681 agcgcatgcg tcccttcatc gaacgcccgg cctttatcga atgtcaccgc ttgagcgcct
     4741 tcggtgagcg ggggaccgac gtcgatgtcg tgctggatct gatgattcac gatctggatc
     4801 tggtgttgtc gttcaatcct gggccggtcg aagaggtgcg ggcggctggc gtgccggtgc
     4861 tctcgtcgaa catcgatatc gccaatgccc gaattgcctt tgcgagcggt tgcgtcgcga
     4921 atgtgacggc gagtcgcgta tcgaccaaca agatgcgccg gctgcgtgtc ttccagcgcg
     4981 accgttacgt ctcgatcgat tttcaaacgc gccaggctgt cgtgtcccgt cgcgtgcagg
     5041 cggccggcgc caagccgacc atcgacatcg aatcttacca ggccggagat gaggagccgt
     5101 tgcggctcga gctggattcg ttcattcacg cggtgaatac cgggacccgt cccgtcgtgt
     5161 ccggtgaaga cggagaagcg gcgcttaatc tggcgaccct ggtcctggag gccatcggcc
     5221 ggtttacgca acgccattcg gcggatgagg ctgcggcggt ggcctttggc cgggagaatg
     5281 tgtaagggta tgacctctgt gccgatctgc acgagacgag cagcatgcca cgcatcttaa
     5341 tcgtcaccgg cgaggcttcc ggcgacctcc atggagctca cttggtcaag gcgctcaagg
     5401 agctgtcccc cgcccttcag attgtgggtg tcggcggcgc gtcgatgctg gcggccggcg
     5461 cggaactcgt gaaagacatt ccccagctgg acgtgatggg gctcatcggc ctgtctgccg
     5521 tcaaaacgat gctgcagcgg atttcgcgta tccggacact gatcaagggt gaacgctggg
     5581 atctcgtcgt gttgatcgac aacccgggcc tcaattttca ttttgcgcga gtggccaggg
     5641 cctgcggtct caaggtgctc tattacattg cgccgcaagt atgggcctgg cggcgggggc
     5701 ggatgcgctg gatccagcag cgcgtcgatc atgtgctcgc gattctgccg ttcgaggaac
     5761 cgctctacaa aaaggccggc gtgcggtgta cgtttgtcgg caatccgctg ctcgacgaag
     5821 tcgcgccgtc gtacgatcgg caggccttgc gccggcagtt cggactgtcg gatgcaggcc
     5881 ccgtgatcgg cttgtttccg ggcagtcgaa aaggtgaact gctcgagcat attccgctcc
     5941 tcctggagac ggtgaagcgt cttgcgcaac ggcacccggc gattcagttt atcctggccc
     6001 aggcgtcatc gattcaagac cagttccttg ccgatctctt gaaagatagc ccggttccca
     6061 ttcgggtctt tcgcaatcag gccagtgaag tcatggcggc ctcggacctg ctggtcgtca
     6121 agtcgggcac gtccaccttg caggctgcgg tggtgggaac gcccatgatt ttgttctatc
     6181 gggcctcatc ctggctcacc taccggttgg cgcgtctcct gattcgggtg ccctggatcg
     6241 gtctcgcgaa tctggtcgcc ggacggggga ttgtgccgga actgattcac gatgaggcga
     6301 cgccggagcg gctcatacag gaaaccgaac ggctgttggg ggatcctcgc gcgtatgaag
     6361 aaatgaaggc tgcgctcttg acggtacgcc aggcgttggg cacacccggt gcgtcgcgtc
     6421 gtgccgcgga agccgtcctg gccgagtgtc gggcatgaaa cattatcttc gtttacttaa
     6481 atatctgaat ccctaccggt ttcgactggg tgcggcgttt ctttgcgcgc tgctcgtggc
     6541 cggactctcc gccgcctatg cctggttggt ccgcccggtc ctcgacggcc tgttcatcag
     6601 caaggatgaa agcctgctgc tcgtgcttcc catcgcgatc ctggcggtgg cggtgctcaa
     6661 gggggtgttc aactacggac agaactatct gatgaactat gtcgggaacc aggtcatcgg
     6721 cgacatcaga gagcagctct tttcaaaact tgttcgactg ccggttcatt ttcacgacac
     6781 gaatacgtcg ggccggttgg tctcgcgggt cattaacgat gtgaaccaga tggccaacgc
     6841 cgtggccggg gtgctgaaag atctgtttca gcagggactg acgtttctgg ccatgatcgg
     6901 cgtgatcatt taccagaact ggaaactggc ggcggtctcg atgatcgtgg tgccgctttc
     6961 cgtggtgacc atggcgagaa tgggaaaacg gttgaggaat ctggcgacgc gcggccagga
     7021 acgcatgggc gacatggcct cgacgctgca ggaaacgctg gccggtatcc gcatggtgaa
     7081 atcgttcggc cgggaagagg aagaagccaa gcgatttcgt ttgagcaacg acgccttcat
     7141 ccatacgacg atgaaggcca ttcaggtctc ctcgctgggt tcctcccata tggaggtcat
     7201 cggtgtgctc ggggtcgcgg ggatcatctg gtacggcggg tatctggtca tccatgatga
     7261 gatgactccc ggcgccttct tctcgttcct ggcggccatg ttcatggcct acacgccgat
     7321 cagaagattg tccggcgcga acaacacggt tcagcaggcg ctggcggccg ccgagcgggt
     7381 attcgatgtg ctggacttgc cgacggagcc ggaggccaac ggcggccaga cggacctgcc
     7441 ggccatttcc aggtccctcg aattccggca ggttgcgttc cagtacgaag gccagggtga
     7501 gcctgcgctg agcggtatcg atctgacgat ccgggcgggc gagatcattg cctttgtcgg
     7561 cagcagcggg agtggaaaga ccacgctggt gagtctgctc ccgagattct acgatccgac
     7621 cggagggcag attctgatcg acggggtgga tcttcagacc tgcagtttgc ggtcggtacg
     7681 cgggcagatc ggaatcgtct cccaggaagt cgtgctgttc gacgatacgg tgcgcaacaa
     7741 tatcggctac gggcggcagg gtgccacgcc cgacgatgtg atgcgagcgg cccagctggc
     7801 ctacgcgcat gagttcattg tccggatgcc ggccgggtat gatacgttga tcggagagcg
     7861 cgggctcaag ctctcgggcg gtgagcggca gcggttggcg atcgcgcggg cgattctccg
     7921 cgacccgccg atcctgattc tggatgaagc gacgtcggcg ctggacaccc aatcggagcg
     7981 catcgtgcag ctggccctga ccaatttgat gcataaccga acgaccctgg tcgtggctca
     8041 ccggctgtcg accattcaga atgccaaccg catcgtggtg ttggaccggg gcagggtcgc
     8101 ggaagtcggc tcgcacgaag agctcctgcg caagggcggc atgtataagc gcctgcacgc
     8161 gatgcagttt gccgatgcga tctcggagta gggacgcaga cgaaggtgag ggatggcagt
     8221 cctatgacaa ctgatcgatc agcggcggcc cctcagccgc agcccttgct gaagcgagtc
     8281 gtcaacgcgc tcaaggtcgc ggtggtgccg cccgtcggct accagatcat tcagatgcta
     8341 cgccgcacga tgacctggcg caccgaaggc tcagagcatg tgaaccgctt gtttgcagaa
     8401 gagaagcggg tgatcctggc cttctggcat gctcagcagt tgatgatgcc gctggccatt
     8461 ccggggcttg aagcccatgt gttgatcagt cagcatcgcg acggcgaatt gatccgccgc
     8521 atcgtggcac ggttcgggtt ggacgccgtt cgcgggtcca gtaccagagg gggtgcggaa
     8581 gcctttcggc agctgattcg cctcgggcgg tccggcggca atctcgtgtt gacgccggac
     8641 ggaccgaagg gcccgcggca ggtggtcaag gtgggcgtag tgcagctggc gagggccacc
     8701 ggcctgccga tcatcccgat ggccttcggc tgctcaaaaa aaaactcttc gcgagctggg
     8761 accggttcat catgccctat ccgttctcgc ggggcctctt tctcctgggg ccgccgatca
     8821 gcgtgccgcc cgacgcctcg tccgacgacc ttgagcgtgt ccgccgggag ttggaagaca
     8881 cgctgaaccg catgaccatc aaggccgatg aggccgtcct gcgggctggc ccgtgatgtg
     8941 gtatctgctt tacaacagtc ttctactcct ggtttccccg attatcctgt tcgtcttgtt
     9001 ggccaagcag cgttgtcgcc gggggttgcc ccaacggctg ggactgcggg cggagatgcc
     9061 cacgactgat ccagccggca ggtcgggttg catctggatt catgccgtgt ctttgggcga
     9121 ggtggtcgcc gtggcgccgc ttgtgcggga tttgcgtcgg cggtaccctg agacgaggtt
     9181 ggtggtctcg accgtaaccg aaaccgggcg tgaagcggtg gagcaacggc tggagggcgt
     9241 ggccgagcac cggtacgcgc cgctcgattt tccctgggtc gtcaatcagg cgatcgaccg
     9301 tctcaagccg agtctgtaca tttttgtcga aacggaactc tggccgaata tcctgcggag
     9361 tttgcggcgg cgcaatgtgc cgtcgattct ggtcaacgga cgattgtcca cccgttcctt
     9421 tgagcggcaa cgcctgccgg tcatccggga tttctaccgg accatgctga acatgatcag
     9481 ctgttgcctc atgcaatcgg agcgggatgc gcagcgcatg atcgacttag gggccgaacc
     9541 gtcgcgggtt cgatgtacag gcaacatcaa gttcgatcaa ccggttcctc aggctgccgc
     9601 cggcggcacg gccgtgtcga aagcggcgct cgggctgacc gaccgggaac tgttgctggt
     9661 agccggaagt acacatcccg gcgaggaaga aacgatcgtc aatgcctatc agatcctgag
     9721 tcctgagttc cctgagctcc gcttggtcct ggccccccgc catatcgaac gggcggcgca
     9781 ggtggagcag atgatccagg cgaaaggcct cacggtgacc cggcgtagca ccggcggaca
     9841 agtgccgatg gcgagcacgg gggcgcgggt gttggtcctg gatacgcgcg gggaattggc
     9901 cttgttatat cgagacgcag tggtggcctt tgtcgggggc accctggcgc cggtgggagg
     9961 gcataatctc ttggagccgg cggtctgggg gaaaccggtg ctgttcggtc cgcataccga
    10021 ccattgtgcc gaagtggcgg cgctcctgct gaatgcgcag ggaggactgg tggtacagga
    10081 cgaacaggac cttgcgcagg gcctgcgagc gctccttcgc gatcccgccg cgttgctgcg
    10141 gatggggcag gcggcgcagc aggtcgtggc ggacaaccag ggtgccctcc agcgcagcgc
    10201 ggagatcatc gccacgtttt tgccggcacg agggacctcg aagaacgcgc gggttgaaca
    10261 ggggcgggag ttgtcgagtc ggcaatcgta atggcggcga ccatgtcgag agggctgcgg
    10321 tcctggttgc ggtgggtcgg gtatccctac gaactcgcgg ctcgcctccg gctgtggggt
    10381 tacacccatg gctggttctc cacgcgtcgg ttacctcgtc ccgtcatcag cgtcgggaac
    10441 ctcaccgtcg gtggcacagg caaaacgccg gtcgtcatgt acctggtgga gcgactgacg
    10501 gcacagggca aacgcgtggc gattctgagc cgcgggtatc ggcgtcgcag caacgctccg
    10561 caattgctgg tctccgacgg acaacaggtg ttggtggggc cggacgaggc cggagacgag
    10621 ccctatttga tcgcgcgccg ctgccctcag gccgtcgtgg cggtgggggc gaaccggtat
    10681 gcgctcggcc aatgggtctt ggggcgactg ccggtggatt gttttgtgct ggacgacggg
    10741 tttcagcatg tccaactcca tcgcgatctg aatctactcc tggtggacgc gacggacgcg
    10801 gcgggcatcc aggcggcgtt gccggtcgga cgtttacggg aacccctctc ggcggccgcg
    10861 cgcgcctcgg ccatccttat cacccgggtc gatgaggcgc atggagggga gtcggtgcgg
    10921 tgtctgttgc tcgacgcctg cggctcactt ccgtcgctgg tgcgtgtggg attccgggcg
    10981 gaggagttcc ggcgcgtagg gacgggcgaa cggcttccgt tggatgcgtt tcgcggccgg
    11041 tcggctgtgc tcttcagcgg gatcggcaat gcggaatcct ttcgcgccct ggtggccgga
    11101 ttaggcatcg ctgtgattga gatgctggca tttcccgatc atgtgcacta tacccgtggc
    11161 atgatagaca ccattcgagc caaggcgaaa gcctctggcg ccgatctgct ggtgacgacg
    11221 gagaaagatg ccgacaaggt tgcgccgttg gtagttccgg aggatgcctg ctgggccgtg
    11281 agacttcgca ccgaaattgt gtcggggcag gaggatttgg agcgactgtt gcgactcgac
    11341 tcagcaccag gcaggacggg aggccatgcg taaggaaggt atccgtcgca tcgtcgtccg
    11401 cggccccaat tggctcgggg atgcagtcat gtgcgaaccg gccttgagtc aggtgcgcac
    11461 cctgttcccg caggccgaga ttaccctgct tgtgaagccc ggcattgcgg atctgctggc
    11521 gcaacatccg gaggtgaacc ggacgctggt gtatgacgat cggggacgcc atgccgggct
    11581 ggttggaaaa tggaccctcg ccgcggtgct gcgccgtcac cgattcgatc tggccatcct
    11641 gtttcagaat gcctttgagg cggcgctgat cagtttcctg gcgggcattc ccagacgatt
    11701 cggatatgcg acggacgggc gaacgctgtt gctgaccgac ccggtgaccg tgccgccacg
    11761 cactgcgcaa cgacatcagg tcgagtatta ttgggatctc ctgaagccgc tgggcgggca
    11821 cggtcctgcg cctgcgcctc gtttgtttgt c
//
LOCUS       DAOQRM010000076        11778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_4599_length_11778_cov_17.347266, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000076 DAOQRM010000000
VERSION     DAOQRM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11778
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4599_length_11778_cov_17.347266"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..369)
                     /locus_tag="PKK81_07680"
     CDS             complement(4..369)
                     /locus_tag="PKK81_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HNV32794.1"
                     /translation="MMHVDFSYRFDARGRTAGTDEADHIRDLIEQVLFTSPGERVMRP
                     DFGSGLLQLVFAPNSEVLAATTQVLVQSVLQRWLGELIVVEGVRVEAVESTLRVIVQY
                     VIRRTGTRVTGTFVQGGGA"
     gene            complement(372..695)
                     /locus_tag="PKK81_07685"
     CDS             complement(372..695)
                     /locus_tag="PKK81_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32795.1"
                     /translation="MPGFLLHLGATVMCAHAGQATPVTPNPRVLVSGQPTVPMTSLYA
                     VAGCTLPPPAAANGPCVTAQYITAAARITSNGVPLLLIDSQAICTPSGTPLLATVTQT
                     RVTGV"
     gene            complement(917..1432)
                     /locus_tag="PKK81_07690"
     CDS             complement(917..1432)
                     /locus_tag="PKK81_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HNV32796.1"
                     /translation="MSEAQKYYGKHRGTVLNNIDPMQMGRLQVQVPDVAGLIPTSWAM
                     PCFPASGKQMGVYMLPQIGSGVWVEFEQGNIDYPIWSGCWYGSVAEVPALGLAGIPVS
                     PNMVIQTAAQNAIVISDVPGPTGGIMLKSATGATLIVNDTGIYIQNGKGAMVTLVGPA
                     VTINNGALTII"
     gene            complement(1429..2553)
                     /locus_tag="PKK81_07695"
     CDS             complement(1429..2553)
                     /locus_tag="PKK81_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32797.1"
                     /translation="MLKGIHLTLLAGPVLPFPVPQPVLEALTEVEVTSATGTAGGFQL
                     RFTIQNKSALQQTFLVAATRTPLMRVILVATINLIPHVLMDGVITNQEITSGEQPGES
                     TLTITGEDLTKVMDLQTFDGLPFPAMPANLRVMTILAKYAVFGVIPLAIPPIGFDVPI
                     PTIRIPSQQGTDLNYILQLAEEAGYVFYIDPGPVPGTNTAYWGPQVKVGVPQPALNVD
                     MDFDRNVESLNFQFNSTKKDLPIVMIHNALTKVPIPIPIPDINPLQPPLGLLGPPVTK
                     LSMLKATGKLSAPAALNEGLKASSESMEAVTGSGSLDVGRYGRLLKARGLVGVRGAGL
                     AFDGLYYVESVTTTIKRGACKQSFRLTRNGLVSLTPMVPA"
     gene            complement(2546..2905)
                     /locus_tag="PKK81_07700"
     CDS             complement(2546..2905)
                     /locus_tag="PKK81_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNV32798.1"
                     /translation="MGDPLQALLDGAMKPNPFPPTSRYYNVQTTSRLAPDGTMQVYLR
                     RRVVPGSDRFTVVQEHRVAAGERLDHLAATYLDDPEQYWRLCDANDAVRPDALLERLD
                     SVVDIALPQGTTGVPGA"
     gene            complement(2938..3588)
                     /locus_tag="PKK81_07705"
     CDS             complement(2938..3588)
                     /locus_tag="PKK81_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32799.1"
                     /translation="MSSFPGSPKLLKGGIVLIDPATAQVQRIIALQYNPESLSRTLQV
                     QGAGEGGDRSEALRLKGPAVETIKLEAEIDATDEMEAGDRTAGEAGIAPQLAALETLL
                     YPSTGQLTTNHMLSSLGTLEILPMETALPLFVFGQNRVIPVRVTEFSVTEEAFDPNLN
                     PIRAKVSLGMRVLSIDDVGFTHKAGSLFMSYLQQKEGLRAKAQGGTLGALGLTGIP"
     gene            complement(3640..5145)
                     /locus_tag="PKK81_07710"
     CDS             complement(3640..5145)
                     /locus_tag="PKK81_07710"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNV32800.1"
                     /translation="MRTQTIHQRATAESGVGSHAGFLQRACACGGSSGLSGTCEECEK
                     KKLIGMSAQAKLRINEPGDQYEQEADRVAEQVMRMPIDGAAPSQRSSDQLIQRRLSGA
                     GGLLSRAAAEEPAPVGEQPASDGAEPKEGTTDEGGSRCPSWRADPQSISKRAAENYVQ
                     HDITPTSQAKVEKINCEPPIANGNYGCFVHFSDGLVVRVIVRATDIVVGMGPGQISTE
                     TPPAATPLCFYDYHCPDGFLVLTKRECKSAKASAAHPPGVPSAVVQRRVAPGQSASLV
                     APPLVHEVLGSSGHPLDQPTRGFFESRFGHDFGHVRVHADAAASESAQAVNALAYTVG
                     SHVVFRTGQFDPQSISGRRLLAHELTHVVQADAGSGAKGSMRRKAVISKDQKEPQPLP
                     PVDCAVEITPAQDCFDLIGDMIAIQADMRENNQWLERYRNGDIPWDTDAYTARARYHG
                     ELRDKLNAKERVRGACCPEQEVPGEAPTAPSAATPDPGVSQAQPDQDGAGR"
     gene            complement(5152..5466)
                     /locus_tag="PKK81_07715"
     CDS             complement(5152..5466)
                     /locus_tag="PKK81_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32801.1"
                     /translation="MTTTRSLPVMPIPIMRQTHGRYSAVFVWLKSEGLSKAGPDTTDV
                     KLLNDWLRKHGIKLGGSHQVGEEMVLLSPTKLTSMPNNRDHEIFEGIVHLRRYCGIKD
                     IP"
     gene            complement(5463..6737)
                     /locus_tag="PKK81_07720"
     CDS             complement(5463..6737)
                     /locus_tag="PKK81_07720"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNV32802.1"
                     /translation="MIARTVTGQTKRILRGQTGLTGGLLPRLGSIGDRFEQEADQVSR
                     EVMLLPGAPGKVTAESSPPPVVQRHLSGSEATKEGVAPPIVQDVLAVPGQPLDGATRV
                     FFEPRFGHDFGNVRVHADERAAESARAVHASAYTVGRDIVFASGRYNQSTVEGRRLLA
                     HELSHVVQQGSSGPQETLQRSGPAQPGNEDERTHRSAGLDVGLEVVISGELFNLVPIP
                     AAVTPESPAWQYRDSAQDYLGAYPNLGNGAWAFIVRQRDGVLCDIGGNCLGWALGNYG
                     LIDPPDQVWGLARQYLDSVGRSVRERRSPLETYMQQVNKGQSEAGAIWDYYMATQFQA
                     VPTESEGEANLALYGRGFSGPMDGPSHIAFRTAAGELWASKPSPSKSPVLHETAAQMT
                     GGQTGEVLRLYTRATGPMSHVVLRERQAEVNP"
     gene            complement(6749..8269)
                     /locus_tag="PKK81_07725"
     CDS             complement(6749..8269)
                     /locus_tag="PKK81_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNV32803.1"
                     /translation="MSATATVQSAAKAPGSAPSGSLIQRQCACGGSAGLSGECEECRS
                     RKLLGRPLQKKLVIGNPEEAFEQEADHVAAQVMRMPHPHATTEAEQRDPAPIVRRRVD
                     GFTGTGAGPAPSIVKDVLASPGRPLDGGTRAFFEPRFGHDFGQVRVHHDDEAAESADA
                     VAALAYTSGRHIVFAQGQYAPHGSGGRQLLAHELTHVIQQGGTQAAFTIQRQCVTGRA
                     CTQPISGDPARFNASEYAAEESGRTARAEESRRDPGAVVASGHGSRAVHVEAFAAAEG
                     IDLSAFYGVLIDRDMGASTGAAAGHCASLAGAVPPFSGPRDAYCMFVPEDMERLTGEA
                     TASPPPRLVGGYPRAAWLQWVREMLVHEAQHIRYDTTAHPDLGGSCTRSTPLYRAANG
                     TEYPLDFYLSELSAILAEFEPTFASVRRRTPAGDYGALLDNLQSAYHDALFSQYESIS
                     GILTTLRCHCDCADADAFVRDTFEFTSTGWSDTTRGMFLDFIQSRIPIVQWPDSGP"
     gene            complement(8266..8520)
                     /locus_tag="PKK81_07730"
     CDS             complement(8266..8520)
                     /locus_tag="PKK81_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32804.1"
                     /translation="MRRVIVTIDRLTLKGVRYEDRHAVAQGLQEQLAALFAEPGMAER
                     LVSLGDVPRLRVDSISSTGEATPRQVGSDAADGIAKGVGR"
     gene            complement(8517..10448)
                     /locus_tag="PKK81_07735"
     CDS             complement(8517..10448)
                     /locus_tag="PKK81_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32805.1"
                     /translation="MSDRVAYDTWVQANQRCLVGEFDRLKARLTGGESAETAGHNIDE
                     IDAEGPAQAAIDVLTNLFGLSRFERDVLLLCAGVEMNSELARVCGEALAPGHRSSVTF
                     GLALAALEAPHWSALTPVGPLRRWRLVELDESPGVANARLRIDERVLHYLAGVNYLDP
                     RLRPLLRTRQPGELLAVAHRQTAATILSAIEAGRSSSGLVLLTGDDLQGQGDVAASVA
                     SELGLQLYMLPAALVPPSASEIEALAVLWQREAFLLHAALLVECAEHEVPKQAGSFID
                     QLGGLVFVIGQELPPLTRQAVPQVVNRPQAVEQRALWEQALGQDAALVNGSLDGVSWQ
                     FRLSAGTIATTGAQVRKRLAGDERPEGLLWRACRTAGRSKLDDLAQRIEPVADWEALI
                     VSVPQQTTLRAMAAQVRHRLTVYQDWGFGSHGTRGLGITALFAGESGTGKTMAAEVLA
                     NELNLDLYRIDLSAVVSKYIGETERNLRRLFDAADESGAILLFDEADALFGKRSEVKD
                     SHDRYANIEVSYLLQRMEAYRGLAILTTNMKAALDQAFHRRLRFVVHFPFPDAAQREA
                     IWRTMFPTSAPVEGLDYAKLARLHMAGGSIRNIALNAAFLAAQAREPLRMGHLLQAAQ
                     SEAAKRDRPLSDAETRGWV"
     gene            complement(10445..>11778)
                     /locus_tag="PKK81_07740"
     CDS             complement(10445..>11778)
                     /locus_tag="PKK81_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HNV32806.1"
                     /translation="LFGAPPGEGRVSSALAIAGVTAVLRDLLNDGLINHNVSGLLGST
                     VTVSAVPPDRVVPANGTESTQLNLFLHQVTFNTGWRNHALPSRDGSGTQRLSNPPLAL
                     DLHYLVSAYSAEELGSEILLGYAMQLLHETPVLDRKAITTALNPSPSVETTLPPALRA
                     LAECGLADQVEQIKLVPDPLSTEEMSKLWTAVQSHYRPTAAYVATVVLIESSKPTKPT
                     LPVLTRGPVDPVTKLERGVVVQADLLPPFPTIQTVQAIDGQPVAAVGATVELTGHHLN
                     GTNRTVVFSNARFDIVHEVAALAGDSASQLQVVVPPSLAVGFYRVAVRLVRPTETEPR
                     ISNQLGLIVGPLITTALPLTVPRDGSGRATITLGCAPDVRAGQSVSLLLGSREVLPES
                     WSGPADSFTFILEQAPVGDHLARLRVDGIDSPLIDRAAEPPVFFNYRVTIT"
BASE COUNT         2129 a         3905 c         3562 g         2182 t
ORIGIN      
        1 acgtcacgct ccgcctcctt gcacgaaggt ccctgtcaca cgagtgccgg tgcgccggat
       61 gacgtattgc acgatgaccc gcaacgtcga ctccaccgcc tccaccctca caccctccac
      121 cacgatcaac tcacccagcc agcgctgcaa tacgctctgc accagcacct gggtcgtggc
      181 ggccagcacc tcgctattgg gcgcaaacac cagttgcagg agaccactgc cgaaatccgg
      241 ccgcatcacg cgttcgccgg ggctcgtgaa gagcacctgc tcgatcaggt cgcgaatatg
      301 atcggcctca tccgtcccgg cggtccggcc gcgagcatca aaccgatagg aaaaatcgac
      361 gtgcatcatc gtcagactcc cgtgaccctg gtttgcgtga cggtcgcgag aagcggcgtc
      421 ccggagggcg tgcagatcgc ttgactatcg atcagcagca gcgggacgcc gttgctggtg
      481 atcctggccg ccgccgtgat gtactgcgcc gtgacgcagg ggccattcgc cgcggcaggc
      541 ggcggtaagg tgcagccggc gacggcatag agactggtca ttggcacggt cggctgcccg
      601 ctcacgagca cgcgcggatt cggcgtcacc ggcgtcgcct gcccggcatg ggcgcacatg
      661 accgtcgccc cgagatgcag taaaaagcct ggcattagct caccctccat gaatggttcg
      721 caggctgcac acaactattc gcgcacacac gccacaatga ttcgcttcgg gccggaagcg
      781 gcatatcctt ccgcatgatg ctcaaaacga ctgtccggca aggccgcagt cagtgaaggc
      841 ccgaggcgta cccttgcggt acgttgaggg cttgaacgca acgagaacgc cgctggcggg
      901 cgttttcagc atcatgctaa atgatcgtga gggcgccatt gttgatggtg accgccggcc
      961 cgaccagcgt caccatcgcg cccttcccgt tttgaatata gatgcccgtg tcgttgacga
     1021 tgagcgtggc gccggtcgcg ctcttgagca tgatgccgcc ggtcgggccc ggcacgtcgc
     1081 tgatcacgat ggcattctga gctgccgtct gaatcaccat gttcgggctg accggaatgc
     1141 ccgccaaccc aagcgccggg acctcggcca cgctcccgta ccagcaaccg ctccagatcg
     1201 ggtagtcgat gttgccctgc tcgaactcca cccagacccc cgaaccgatc tgcgggagca
     1261 tgtacacccc catctgcttg ccgctggcgg gaaaacaggg catggcccag gaggtcggaa
     1321 tcagtccggc gacgtccggc acctgtacct gcaatcgccc catctgcatc ggatcgatgt
     1381 tgttcaacac cgtgccacgg tgcttgccgt aatatttctg ggcctcactc atgccggcac
     1441 catcggcgtc agcgacacaa ggccgttccg cgtgagacga aaactctgct tgcaggcccc
     1501 gcgcttgatg gtcgtggtca cactctccac gtaatacaac ccgtcgaacg ccaatcccgc
     1561 gccgcgcacc ccgaccaggc cccgggcctt caggagccgg ccatagcgcc cgacatcgag
     1621 cgacccgctg cccgtcaccg cctccatcga ctcgctcgaa gccttcaacc cttcgttgag
     1681 ggctgcaggc gcggagagtt tgcctgttgc cttcaacatc gacagtttcg tgacgggcgg
     1741 tcccagcagg cccaggggag gctggagcgg attgatgtca gggatgggaa tgggaatggg
     1801 caccttcgtc agcgcattgt gaatcatcac gatcggtaga tccttcttgg tcgaattgaa
     1861 ctgaaagtta agcgattcca cgttgcgatc aaaatccata tcgacgttca gcgccggctg
     1921 cggaaccccg accttgacct gcggtcccca gtaggcggtg ttggtacccg gaaccgggcc
     1981 cgggtcgatg tagaagacat accccgcttc ttccgccagt tggaggatgt aattgagatc
     2041 ggtcccctgc tgggacggaa tccggatcgt gggaatcgga acatcgaaac cgatcggtgg
     2101 aatcgccagg ggaatcaccc cgaagaccgc gtacttcgcc agaatcgtca tcacccgcag
     2161 gttggcgggc atggcgggaa agggcaaccc gtcgaacgtc tgcaggtcca tgaccttggt
     2221 cagatcttcc ccggtgatcg tcaaggtcga ttcacccggt tgctcgccgg aggtgatttc
     2281 ctgattggta atcaccccgt ccattaatac gtgcgggatc aggttgatag tggccaccag
     2341 gatcacgcgc atcagcggcg ttcgtgtcgc cgcgacgaga aacgtctgct gcaaagccga
     2401 tttgttctga atcgtaaatc gtaactgaaa cccgccggct gtgccggtgg cactcgtcac
     2461 ttcgacctcg gtcaaggcct ccaagaccgg ctgaggcacc ggaaagggca gcaccggccc
     2521 ggcaagcaag gtgagatgga tgcctttaag caccggggac tcctgttgtg ccttggggca
     2581 gcgcgatgtc gaccacgctg tccaaccgtt ccagcagcgc atccggccgc accgcatcgt
     2641 tggcatcgca taaccgccaa tattgctccg ggtcgtccaa ataggtcgca gccagatggt
     2701 cgaggcgctc tccggccgcc acccgatgtt cctgcacgac ggtgaaccgg tcgctccccg
     2761 gcacgacgcg ccgccgcaaa tacacctgca tggtgccgtc cggagcgagc cgcgaggtgg
     2821 tctgcacgtt gtaataccgg ctggtcggcg gaaacggatt cggcttcatc gcgccgtcca
     2881 acaacgcttg taatggatcg cccatgtcgc gtgcctccta tcgaccgaaa cagattctta
     2941 cgggatgccc gtcaggccga gcgcgcccaa ggtgccgccc tgcgccttcg cgcgcagccc
     3001 ttccttctgc tgcagataac tcatgaagag actgccggcc ttgtgcgtaa accccacatc
     3061 gtcgatcgag agcacccgca tcccgaggct caccttcgca cggatgggat tcaagttggg
     3121 gtcgaacgcc tcctcggtca cgctgaactc ggtgacccga accgggatga cgcggttctg
     3181 tccgaacacg aacagcggca aggccgtctc catcggcaga atttcaaggg tgccgagact
     3241 ggagagcatg tggttggtcg tgagttgccc cgtgctcggg tagagcaagg tctccagcgc
     3301 cgccaactgc ggtgcgatgc ccgcctctcc cgcggtgcga tcgcccgcct ccatctcgtc
     3361 cgtcgcgtcg atttccgcct ccaacttgat cgtctctacc gccgggcctt tgagacgcag
     3421 cgcttccgaa cgatcgcccc cctcgccggc gccctgcacc tgcagcgtgc gactgagcga
     3481 ctcggggtta tattgcagcg cgatgatccg ctgaacctgc gccgtggccg gatcgatcag
     3541 gacgatgccg cccttgagaa gtttgggaga accgggaaag ctactcatga ccacatcact
     3601 cctcgatctc acgcatcaaa tcgtcggctc cacgacccgt catcgaccgg ctccgtcttg
     3661 atcgggttgc gcctgcgaca ctccaggatc cggggtcgca gccgaaggcg cggtcggggc
     3721 ttcccccgga acctcctgct caggacagca ggcaccacgc acccgttcct tcgcgttcaa
     3781 tttgtcgcgt agttctccgt ggtatcgagc gcgcgccgta tacgcatcgg tatcccacgg
     3841 aatgtcgccg ttcctgtaac gttccagcca ctggttgttc tcgcgcatat cggcttggat
     3901 cgcgatcatg tccccgatga gatcgaaaca atcctgcgca ggagtgatct ccacggcaca
     3961 gtcgaccggg ggcaacggct gaggctcttt ctgatccttc gatatgacgg ccttcctccg
     4021 catcgatccc ttcgcccccg agccggcgtc tgcttgcacg acatgcgtca gttcatgcgc
     4081 cagcaggcgg cgaccgctga tcgactgtgg atcgaattgc ccggttcgga acacgacatg
     4141 cgacccaacc gtataggcca acgcattgac ggcctgcgcc gattccgagg ccgcggcatc
     4201 cgcatgcaca cgcacgtggc cgaagtcgtg cccgaagcgc gactcaaaaa aaccgcgtgt
     4261 cggctgatcc agcggatgcc ccgacgaccc caatacctca tgtaccaacg gcggcgcgac
     4321 gagagaagca gactgacccg gagccacgcg tcgttgcacg accgccgatg ggacgcccgg
     4381 cggatgcgcc gctgaggcct tggcgctctt acactcccgc ttggtgagga cgaggaaccc
     4441 atccgggcag tggtagtcat aaaagcacaa gggcgtcgcg gccggcggcg tttctgtgga
     4501 gatttgtcct ggtcccatgc cgaccacgat atcggtcgcg cggacgatga ctctcaccac
     4561 caggccgtcg ctgaaatgca cgaaacagcc gtagttgccg ttggcgatcg gcggctcgca
     4621 gttgattttc tccaccttgg cctgggatgt cggcgtgatg tcgtgttgca cataattttc
     4681 ggcggcccgc ttgctgatgc tctgcggatc ggcgcgccag ctcgggcagc gactgccgcc
     4741 ctcgtcggtc gtcccctctt tcggctcagc cccgtcggac gccggctgtt ccccgaccgg
     4801 tgccggttct tcagcggcgg cgcggctcaa caggccgcct gccccggaga ggcgccgttg
     4861 aatgagttgg tccgacgatc gttggctcgg agcggctcca tcgatcggca tgcgcatcac
     4921 ctgctccgcc acccgatccg cctcctgctc gtactgatcg cctggctcat tgatgcgcaa
     4981 cttcgcctgc gccgacatgc cgatcaattt cttcttctcg cattcctcgc aggtacccga
     5041 caacccgctg gatccgccgc aggcgcaggc ccgctgcagg aagcccgcgt ggcttccgac
     5101 accggactcg gcagtggctc tctggtgaat ggtctgtgtt ctcatgggcg cttacggaat
     5161 gtccttaatt ccacagtatc ggcgtagatg aacgattccc tcgaagatct cgtggtcgcg
     5221 gttgttcggc atggacgtca gcttcgtggg agacaacaac accatttctt cgccgacctg
     5281 gtgacttcct cccaacttga tgccgtgttt tctcagccag tcgttcaata gcttgacgtc
     5341 agtcgtgtcc ggccctgcct tcgacaagcc ttcactcttg agccagacga aaacggctga
     5401 gtaccggccg tgggtctgcc gcatgatcgg gatcggcatg accggaagac ttctcgtcgt
     5461 cgtcatgggt tcacctcggc ctgccgctca cgcaatacga catggctcat tgggcctgtt
     5521 gcccgggtat acagtcggag gacctcacct gtttgtccac cggtcatctg ggcggccgtc
     5581 tcatgcagga cgggggattt cgaggggctc ggcttggacg cccataactc gccggccgcc
     5641 gtgcggaatg caatatgaga gggcccgtcc atggggccgc tgaatcctcg gccgtagagt
     5701 gccagatttg cttcaccctc actctctgtc ggcaccgcct gaaactgcgt ggccatgtag
     5761 tagtcccaga tggctcccgc ctcggattga cctttgttca cctgctgcat gtaggtctcc
     5821 aacggagacc ggcgctctcg caccgatcgg cctaccgagt cgaggtattg ccgtgcgagc
     5881 ccccatacct ggtccggtgg atcaatgagg ccatagttgc cgagcgccca acccaggcaa
     5941 tttccaccga tgtcgcacag caccccatcc cgctgtctga cgatgaacgc ccacgcaccg
     6001 tttcccaggt tgggataggc cccgaggtag tcctgcgcac tgtcgcggta ttgccatgcc
     6061 gggctctcgg gagtcacggc ggcagggatc ggcaccaggt tgaagagttc tccgctgatg
     6121 acgacctcaa ggccgacgtc gagtcctgca gaacggtgcg ttctttcatc ttcgttcccc
     6181 ggctgagcgg gtccactccg ttgcagcgtc tcctgcggtc cgctcgaacc ttgctgcacg
     6241 acatggctta actcatgagc caggagtctg cggccttcca cggtcgattg gttgtatcgc
     6301 ccggaggcaa agacgatatc gcgtcccacc gtataggctg aggcatgcac ggcgcgggcc
     6361 gattccgcag cacgctcgtc cgcgtggacc cgcacattcc cgaaatcgtg gccgaaccgc
     6421 ggctcgaaga agacgcgcgt ggcaccatcc agcggttgtc cgggtacggc cagcacgtcc
     6481 tgcacgatcg ggggcgcgac accctctttt gtagcttccg atccactcag atggcgttga
     6541 accacaggtg gaggcgacga ttccgccgtg accttaccgg gggctcccgg tagtagcatc
     6601 acctcccggg aaacctgatc ggcctcctgc tcgaaccggt cgccgatgct gccgaggcga
     6661 gggagaaggc cacctgtgag gccggtttgc ccccggagga ttcgtttcgt ctgccctgtg
     6721 acggttcggg ctatcatctt ggtgcgcctc acggtcctga atccggccat tgcacaatcg
     6781 gaatgcggga ctggatgaag tctaaaaaca ttccgcgcgt ggtgtcggac cagccggtgc
     6841 tggtgaactc gaaggtgtcg cgcacgaagg catccgcatc cgcacaatcg cagtggcatc
     6901 gcaacgtcgt gaggatgccg ctgatgcttt cgtactgact gaacagggcg tcgtgatagg
     6961 cggattgtaa attgtcgagt aaggccccgt agtcgccggc aggggtgcgt cgccgaaccg
     7021 aggcgaacgt cggctcgaac tcggccagaa tggcgctgag ttcgctcaag taaaagtcga
     7081 gagggtactc ggttccattg gcggcccgat acagcggggt cgagcgcgta caggagccgc
     7141 ccaagtccgg atgagcggtg gtgtcgtacc gaatatgctg cgcctcatgg acgagcattt
     7201 cccgaaccca ctgaagccag gccgcgcgtg gataccctcc gaccaggcgc ggcggcggag
     7261 aagccgttgc ctccccggtc agcctctcca tgtcttccgg cacgaacata cagtaggcat
     7321 cgcgaggacc ggagaacgga ggcacggcgc cggccaggct cgcgcagtgc ccggcggctg
     7381 ccccggtgga cgcacccatg tcgcgatcga tgaggacgcc gtagaaggca gagaggtcga
     7441 tgccttctgc ggcggcaaag gcctcgacat gcacggcccg actcccatgt ccggaagcca
     7501 cgactgcccc tggatctcgt ctggattctt ctgcgcgcgc ggtgcggccg gactcttccg
     7561 ccgcgtattc cgaggcattg aaccgcgcgg ggtcgccgga gatcggctgg gtacaggccc
     7621 tccctgtgac gcattgacgc tgaatcgtga aggccgcctg agtcccgcct tgttggatca
     7681 catgcgtgag ttcatgcgcc aggagctgcc gcccgccgct cccgtggggc gcgtactgtc
     7741 cctgcgcgaa cacgatatgg cggccgctcg tataggccag ggccgccacc gcatccgcag
     7801 actccgctgc ctcgtcgtcg tgatgcaccc ggacctgccc gaagtcatgt ccgaaacgcg
     7861 gttcgaagaa ggcgcgggtt cctccatcca gcggacgacc cggcgacgcg aggacatcct
     7921 tcacgatcga aggggccggc cccgcgcccg tgccggtaaa accgtccaca cggcgtcgca
     7981 cgatcggcgc cgggtcacgc tgttcagctt ctgtcgtcgc gtgagggtgc ggcatgcgca
     8041 tcacctgggc tgccacgtgg tcggcttcct gttcgaacgc ttcctcaggg ttgccgatca
     8101 cgagtttctt ctgtagcggc ctccccaaca attttcgact cctgcattcc tcgcactcac
     8161 cgctcaatcc tgccgatccg ccgcacgcgc attgtcgctg gatgagcgag ccggacggcg
     8221 cgctacccgg agccttggcg gcggattgaa cggtggccgt tgcgctcatc gccccacacc
     8281 ctttgcaatt ccatcagccg cgtcgctccc cacctgtcgt ggagtcgcct cgcccgtgct
     8341 ggagatcgaa tcgacccgca ggcgcggaac atcgcccagg ctcacaagcc gctccgccat
     8401 gcctggttct gcgaacagcg ccgccagctg ttcctgcaac ccctgggcaa cggcgtggcg
     8461 gtcttcatag cgcacaccct tcaacgtcag gcgatcgatc gtcacgatga cgcgtctcat
     8521 acccaccccc tggtctctgc atccgacagg ggccggtccc gctttgccgc ctcgctctgt
     8581 gccgcctgca ggagatgtcc catgcgcagg ggctccctgg cctgcgccgc gaggaaggcg
     8641 gcgttgaggg cgatgtttcg gatgctcccg cctgccatat gcagccgtgc cagcttggcg
     8701 taatccagcc cttcgaccgg tgccgatgtt ggaaacatgg tgcgccaaat ggcttcccgc
     8761 tgcgcggcat cgggaaaggg aaagtggacg acaaagcgca accgccggtg aaaggcctgg
     8821 tcgagcgccg ccttcatgtt ggtcgtaaga atcgccagcc cgcgataggc ctccatgcgc
     8881 tggagcaaat aactcacttc aatgttcgcg tagcgatcgt ggctgtcctt cacctcgctc
     8941 cgtttgccga acagcgcgtc ggcttcgtcg aacaggagaa tggctccgct ttcatccgcc
     9001 gcatcgaaca agcgccgcaa attccgttcc gtctcgccga tatacttgct gacgacggcc
     9061 gagaggtcga tgcggtagag atcgagattc agctcgttgg ccaacacttc ggccgccatg
     9121 gtctttccgg ttccgctctc gccggcgaag agggccgtaa tgccgagccc cctcgtaccg
     9181 tgactgccga acccccagtc ctgatacacg gtcaaccgat gccgtacctg ggccgccatc
     9241 gcccgtaggg tggtctgttg cggcaccgac acgatcagcg cctcccaatc ggcgaccggc
     9301 tcgatccgtt gcgcgaggtc atcgagtttg gaccggcctg ccgttcggca ggcacgccag
     9361 agcagcccct ccggtcgttc gtcccctgcc aatcgtttgc gcacctgcgc acccgtggtc
     9421 gcgatcgttc cggcactcaa gcgaaactgc caggaaactc cgtcgaggga gccgttgacc
     9481 agcgcggcat cctgaccgag cgcctgttcc cacaaggccc gttgctctac cgcctgcggc
     9541 cggttcacga cctgtggaac tgcctgccgc gtgagcggcg gcaactcctg gccgatcacg
     9601 aacaccagac cgccgagttg gtcgatgaag ctacctgcct gtttcggcac ctcatgctcc
     9661 gcgcactcca caagcaacgc cgcatggagc agaaacgcct cccgctgcca taacaccgcc
     9721 agcgcctcga tctccgacgc gctgggcggc acgagcgcgg caggaagcat atacaactgc
     9781 agacccagtt ccgatgccac ggaagccgcc acatcgccct gcccttgcag atcgtcgccc
     9841 gtcagaagaa cgaggcccga cgaggatcgg ccggcttcga ttgcggagag gatcgtggcc
     9901 gcggtctgtc gatgggcgac ggccaacaac tcgcctggtt gtctggtacg caggagaggc
     9961 cgcaaccgcg gatcgagata attgacgcct gccagatagt gcaacacgcg ctcgtcgata
    10021 cggaggcgcg cattcgccac tcccggactc tcatcgagtt ccacgagccg ccaccggcgc
    10081 aacggtccca ccggcgtcaa cgcgctccaa tgcggcgctt cgagtgcggc cagggccaag
    10141 ccgaaggtga cggacgaccg atggccggga gccagggcct cgccacagac acgcgctaac
    10201 tccgaattca tctcgacgcc tgcgcagagc aacagcacat cgcgttcgaa gcggctgagg
    10261 ccgaacaggt tggtgaggac atcaatggcg gcctgggcgg gtccttccgc atcgatctcg
    10321 tcgatattgt ggccggcggt ctccgcagac tccccaccgg tgagcctggc cttcaggcgg
    10381 tcgaactcac cgaccaaaca acgttggttg gcctggaccc aagtgtcgta cgcgacgcgg
    10441 tcgctcatgt gattgtcacc cgataattga agaacaccgg cggctcggcc gcacgatcga
    10501 tcaacggact gtcgatgcca tccacgcgca accgggccaa atgatcaccg accggcgcct
    10561 gttcgaggat gaacgtaaag gagtccgccg ggccggacca cgattcaggc aagacctctc
    10621 ggcttccgag caagagggag accgattgtc cggctcgaac gtccggcgca cagcccagcg
    10681 tgatggtcgc cctgccgctt ccatccctgg gcaccgtcag gggaagcgcg gtcgtgatga
    10741 gagggccgac aatcagccct agctggttgc tgatccgggg ctcggtctcc gtcggacgaa
    10801 cgagtcgcac agccacccgg tagaaaccga cggccaacga cggcggcacg accacctgga
    10861 gctgcgaggc cgagtcgccg gccaaggccg ccacctcgtg cacgatgtcg aatcgagcat
    10921 tcgaaaacac cacggtgcga ttggtaccgt tgaggtgatg tccggtcagt tcgacggtcg
    10981 cccccacggc ggcgaccggt tgcccatcga tcgcctgcac tgtctggatg gtcggaaacg
    11041 ggggcaggag atcggcctgg accaccacgc cccgctcgag tttcgtcacc ggatcgaccg
    11101 gcccgcgggt gagcaccggc aatgtcggct tcgtcggctt cgaggactcg atcaacacca
    11161 ccgtcgccac ataggccgcg gtgggtcggt aatgggactg cacggcggtc cagagtttgg
    11221 acatctcttc ggtgctgagg ggatccggaa ccagcttgat ctgttccact tgatccgcca
    11281 gcccgcattc ggccagggcc cgcaaggccg gcggcaacgt cgtctccacc gacggagaag
    11341 gattcagcgc ggtcgtgatg gccttccgat ctaagaccgg ggtctcatgg agcaactgca
    11401 tggcataacc caacaggatc tcgctaccca attcctcggc gctgtaggcg ctcacgaggt
    11461 aatgaagatc cagggccaac ggcggattgc tgagccgttg cgtgcccgat ccgtcgcggg
    11521 acggcaaggc atgattccgc cagcccgtat tgaatgtgac ttgatggaga aatagattca
    11581 gttgggtgct ttcagtgcca ttggcgggga ccacccgatc cggcgggacg gcgctcaccg
    11641 tgaccgtact gccgagcagg ccgctgacgt tgtggttgat caagccgtcg ttgaggaggt
    11701 ctcgaagcac ggctgtgact ccggcaatgg ccaacgcgct gctcacgcgc ccctcccctg
    11761 gcggcgcgcc aaatagtc
//
LOCUS       DAOQRM010000077        11609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_4721_length_11609_cov_16.564047, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000077 DAOQRM010000000
VERSION     DAOQRM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4721_length_11609_cov_16.564047"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2939)
                     /gene="carB"
                     /locus_tag="PKK81_07745"
     CDS             complement(<1..2939)
                     /gene="carB"
                     /locus_tag="PKK81_07745"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNV32807.1"
                     /translation="MPRRTDIRSILLIGSGPIVIGQACEFDYSGTQACKALKEEGYRV
                     ILINSNPATIMTDPDFADRTYIEPITLDVVEKVIECERPDALLPTMGGQTALNTAIGL
                     AKRGVLEKYGVRLIGASIAAIHKAEDRDAFRQAMWKIGLRVPDSGVATSLAEAERELE
                     RIRFPAIVRPSFTMGGTGGNIAYNIEEFRTQVEWGLSMSPVRQVLIEQSVIGWKEFEL
                     EVMRDLKDNVVIICPIENLDPMGVHTGDSITVAPALTLTDKEYQMLRDAAVRIIREIG
                     VDTGGANIQFGLNPVNGEMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     ITNDITGVTKASFEPTIDYVVVKIPRFAFQKFPGADPTLTTQMKSVGEVMAIGRTFKE
                     SLQKAIRSMEVDQFGFSSKMGLDLAVPPGLDREEAMEQVRKAVRTPLPDRLWRLADGM
                     RLGMPNQELFALTKIDPWFLQQIRELIEFEARLVAERATLGTTGLRPDLLIEAKELGF
                     SDPRLAQLLGVEQQTVRGWRLALSQGAQPRSVTYKRVDTCAAEFEAHTPYLYSTYERE
                     CEARPTDKKKVVILGGGPNRIGQGIEFDYCCVHAAMALREEQIETIMVNCNPETVSTD
                     YDTSDRLYFEPLTEEDVLNIVEREQPMGVVLQFGGQTPLKLALSLSRAGVRILGTSPD
                     AIDRAEDRARFRELLDKLGLRQAESGMAHSVEEALTIAAEITYPVMVRPSYVLGGRSM
                     QIVYDEPGLLHYMNTAVKASEKHPVLIDKYLRDAIEIDADAISDGTTVVVAGIMEHIE
                     EAGVHSGDSACSLPPYTLDATIIEEIRRQMTALALELGVIGLMNAQFAVKDQTIYVLE
                     VNPRGSRTVPFVSKAIGVPLAKLAMKVMVGKSLQQLNFTTAPTPTHLSVKEAVFPFTK
                     FAGVDVLLGPEMKSTGEVMGIDSDFGWAFVKSQAGAGAILPTSGTAFISVKSEDRAGA
                     FDVARRLV"
     gene            complement(2961..3944)
                     /locus_tag="PKK81_07750"
     CDS             complement(2961..3944)
                     /locus_tag="PKK81_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV32808.1"
                     /translation="MMHPSGRHSIDTLLDTPMGRRAALALGVRAAYLLSTTVGLGAAA
                     GLMQSLAGCQRAPGTAREQFIYISEEKEMAMGLSAFREVLRQAPLSDNPELNEMVHRV
                     GNRIAKAANKPEYQWEFAVIQDDRTINAFALPGGKVAVFTGILKVTKTEDGLATVMGH
                     EVAHALQRHGAERMSRSVLEQIGQLAALGAGAAAGRPDAAMAAMTVYGVGVSLPFDRR
                     QESEADFIGLRLMAEAGYDPREAVAFWERMSGCPRAMINKLCFRSQQAIPEFLSTHPS
                     DVTRINQIEAWIPDAMKHYHPAGKVPAIPSGPIQPYRPPVGPMPEAPLPTG"
     gene            complement(3992..5068)
                     /gene="sppA"
                     /locus_tag="PKK81_07755"
     CDS             complement(3992..5068)
                     /gene="sppA"
                     /locus_tag="PKK81_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNV32809.1"
                     /translation="MHRSLPDVDLSAVANDLQPNARPHLRRLDMNRKVGQALSPWHLC
                     TAVAVLVLALMQAACVTINLPPGPGALEEHKVSGTGKDKVLLMDVSGVISSENKDGFY
                     SSPGMLATVKEELERATKDERIKAVVLRINSPGGTVTASDIIYHELKSFKTSRKIPIV
                     ASIMDVGASGGYYIAAAADTVLAHPSTVTGSIGVIMLTVNARGLLEKVGVETNAVTSG
                     PRKDMGSPFRAMLPEERAIFQGVIDGFYQRFLQVVQDGRPNMNGETIRKLADGRIYSG
                     EQAKASGLVDDIGYLEDAIELAKKKAGLTEARVVTYRRPGEYQNNVYSRLVAPAPSLA
                     SLANLDVLSVVRGGSPQFMYLWMP"
     gene            complement(5061..6245)
                     /gene="carA"
                     /locus_tag="PKK81_07760"
     CDS             complement(5061..6245)
                     /gene="carA"
                     /locus_tag="PKK81_07760"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNV32810.1"
                     /translation="MKKAILALADGTWFEGRALGAEGETGGEVVFNTAMTGYQEVLTD
                     PSYRGQIVTMTCPHIGNYGVTPEDIESTRIWAEGFVVKESSRLASNWRSKATLQEYLQ
                     AANIVAIEGIDTRALTTHLREKGAQPGVISHIDLDPRRLADKARKAPSIIGRDLAATV
                     TCERRYTWTAGTGDWAPKLTMPEPGAAQAARKTWRVVAYDFGVKQNILRRLVDVGCEV
                     TVVPASTPAKDVLALNPQGLFLSNGPGDPEGVPYAMDALRELIGRLPIFGICLGHQLL
                     GLALGFSTYKLKFGHHGANHPVIDLRTRKVEITSQNHNFAVRFPIGAPAQGQAMPIVD
                     TPFGRVQLTHTSLNDGSVEGMMCLDRPVFSVQYHPEAAPGPHDSAYLFEQFVALMETH
                     YA"
     gene            complement(6255..6758)
                     /locus_tag="PKK81_07765"
     CDS             complement(6255..6758)
                     /locus_tag="PKK81_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNV32811.1"
                     /translation="MRQGLIACITCELLALAVWIGGLLVLVAAVIPAVFNTFGGQDTG
                     GFFLTRAFDGYNRLVLGSAAILTSGVLWRAWLVQRGLADGEITRTEWLLLGAMLLTAG
                     VITFVLHPQAAALQAQAFASKGEEARKAAFEAFFQLHKPVRALYIVNVGLGIALLTVR
                     VRSWVPR"
     gene            complement(6771..8063)
                     /locus_tag="PKK81_07770"
     CDS             complement(6771..8063)
                     /locus_tag="PKK81_07770"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNV32812.1"
                     /translation="MTLLIQGGHVIDPGRVNGVADVLIENGTISAVGPALTAPAGATV
                     IQAKGQLVLPGFVDLHVHFREPGFEYKETIQSGTAAAVAGGFTTVCAMPNTNPVNDNQ
                     AVTEFMLERAKAAGNAHLYPIGAITKKSEGKELAEIGDLRRAGCVAISDDGKPVMNSL
                     VMRRAMEYARAFDVPVVDHCEDLHLSEGGCMNEGLVSTELGLPGIPSAAEDVMVARNV
                     SLAELTGARLHLAHISTAGSVRMVREAKARGLKVTAEACPHHFTLTEELTRGYNTHAK
                     MNPPLRTMQDVQAIKEGLRDGTIDVIATDHAPHATQEKQQEFTEAPFGIVGLETALSL
                     TLALVDEGVLTLESAVDKLATAPAKAFSLNAGTLAVGAPADVAIVDPNREWQVDPSRF
                     RSKSRNTPFAGWKVKGRVTTTIVSGRVVFELDRSERQA"
     gene            complement(8119..9039)
                     /locus_tag="PKK81_07775"
     CDS             complement(8119..9039)
                     /locus_tag="PKK81_07775"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNV32813.1"
                     /translation="MGLKRKDLLSLTDLSADDITLILETADSFKEVSGREIKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSSSSVVKGETLLDTARNIEAMQA
                     DIIVLRHSSAGAAETLARGVKSSVINAGDGWHEHPTQALLDLYTIRSRRMGFAGLRVA
                     IVGDVAHSRVARSNIFALTKLGAEVRVVGPPTMIPLQISQLGVQVYHNLDEALRGVNV
                     IMMLRLQLERQGRALFPTIREYARHYGLTSERVKLAETGAIVMHPGPINRGVEIAPDV
                     ADSLSSVILDQVANGVAVRMGILYLMSGAG"
     gene            complement(9054..9614)
                     /gene="pyrR"
                     /locus_tag="PKK81_07780"
     CDS             complement(9054..9614)
                     /gene="pyrR"
                     /locus_tag="PKK81_07780"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNV32814.1"
                     /translation="MTTEPTTGNRQERVVMDAGDIARAVTRIAHEILERNKGIKDLAL
                     VGIRTGGVHLAHRLVRRIHDIEGTQIPIGELDITLYRDDLSLRKDQPILRKTSVPFKI
                     SDLKVVLVDDVLFTGRTIRAAMDSLIDLGRPAEIQLAVLVDRGHRQLPIKANYIGKNI
                     PTSREEAIEVHLEENGEEDRVVILRA"
     gene            9944..>11609
                     /locus_tag="PKK81_07785"
     CDS             9944..>11609
                     /locus_tag="PKK81_07785"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNV32815.1"
                     /translation="MTASAASTATPDLLTALANKATHLRMDSVKATTEAGSGHPSSCC
                     SAADIVAVLFFSVMRYDPKNPKLPNSDRFVLSKGHAAPLLYAAWAEAGLFPKSELLKL
                     RTLGSDLEGHPTPRLSFVDMATGSLGQGLPAGVGLAFNAKSIDKTDYRTYVLMGDGES
                     VEGSVWEAAEVARHAALDNLCAIVDVNRLGQSDPTMLQHDMEAYRARWAGFGWHAIVV
                     DGHDIGALVAAFEEAARTKGRPTVLLAKTFKGRGISFMENHPEWHGKPMKKGEETQKA
                     LDELTRQLKPGSTQPQIPMPTAVKAAAPAKGTMAPPPYKLGDSAATREAFGAALLALG
                     EANSQVVALDADVKNSTYSDKFGKRFPDRFLENFIAEQNMLGAAAGIAACGKIPFVAT
                     FAAFFTRAYDFIRMAAISQSNIKLVGTHVGVSIGEDGPSQMGLEDLAMMSAQPGVTVL
                     YPSDALSMYKLVEAAAAHKGMVYLRAGRPKTPVIYGVDEQFRIGGSKVVRQSAADQLT
                     IVAAGVTLFEALKAHDQLKAAGITTRVVDLYSIVPVDRATLLDCARA"
BASE COUNT         2118 a         3545 c         3579 g         2367 t
ORIGIN      
        1 acgagccgcc gcgcgacatc gaaggcccct gcccggtctt cgctcttcac gctgatgaag
       61 gcggtgccgg aggtcggcag aatggcgccg gcaccggcct gggatttgac gaaggcccat
      121 ccgaaatcgc tgtcgatccc catgacctca ccggtggatt tcatttcggg gcccaggagc
      181 acatccacgc ctgcgaactt cgtgaaaggg aacacggctt ccttcaccga gagatgtgtc
      241 ggcgtcgggg cggtggtgaa attcagctgt tgcagcgact tgccgaccat caccttcatc
      301 gccaatttgg cgagcggaac gccgatggcc ttgctgacga agggcacggt ccgcgacccg
      361 cgcggattga cctccagaac ataaatcgtt tggtccttca cggcgaattg cgcattcatg
      421 agaccgatga caccgagttc caatgccagc gccgtcattt gccgccggat ctcctcgatg
      481 atcgtcgcat ccagcgtgta ggggggcagc gagcaggccg aatcgcccga gtggacgccc
      541 gcttcctcga tgtgctccat gatccccgcg acgaccacgg tcgtgccgtc ggaaatggcg
      601 tccgcgtcga tttcgatggc atcgcgcaaa tacttatcga tcaacaccgg atgtttctcc
      661 gatgccttga ccgccgtgtt catgtagtgg agcagtcccg gctcgtcata gacgatttgc
      721 atcgaccgtc cgccgagcac atacgacggc cgcaccatta cgggataggt gatctcggcg
      781 gcgatcgtca acgcttcttc gacggagtgg gccatcccgc tttcggcctg acgcaacccg
      841 agcttgtcga gtaactcacg gaaacgggcg cgatcctccg cgcggtcgat tgcatccgga
      901 ctggtgccga gaatcctgac accggcgcgg gacagggaga gcgccagttt caagggcgtc
      961 tgtcccccga attgcagcac cacacccatc ggttgttcgc gttccacgat gttgagcacg
     1021 tcttcttccg tcaacggttc gaagtagaga cggtcggagg tgtcatagtc cgtgctgacc
     1081 gtttccggat tgcagttgac catgatggtt tcaatctgct cttcccgcag ggccatggcg
     1141 gcgtgcacac agcagtagtc gaactcgatc ccctgcccga tccggttcgg tccgccgccg
     1201 agaatgacga ctttcttttt gtcggtcggc cgggcttcac attcccgctc gtaggtcgag
     1261 tagagatacg gcgtatgggc ttcaaattcg gccgcacagg tatcgacccg cttataggtc
     1321 acacttcgcg gttgagcccc ttggctcaat gccagccgcc agccgcgaac ggtctgttgt
     1381 tccactccca ggagttgagc cagacgtggg tcggagaagc ccagttcctt ggcctcgatc
     1441 aggagatcag gccgcagacc tgtggtgccg agtgtcgccc gttccgcgac gagtcgggcc
     1501 tcgaattcga tcagttcacg aatctgttgc agaaaccagg gatcgatctt ggtgagcgcg
     1561 aataactcct gattgggcat gccgagccgc atgccgtcgg ccagccgcca gagccggtcg
     1621 ggcagcgggg tgcgcaccgc cttgcggacc tgctccatgg cctcttcccg gtcgagaccg
     1681 ggtgggaccg cgagatccag ccccatcttc gagctgaatc cgaactgatc gacctccatg
     1741 gaccggatcg ctttctgcag cgattccttg aaggttcgtc cgatggccat cacttcgccc
     1801 accgacttca tctgtgtggt cagggtggga tcggctccgg gaaatttctg aaaggcaaag
     1861 cgcggaatct tgacgacgac gtaatcgatg gtcggctcga acgaggcctt cgtcacaccg
     1921 gtgatgtcgt tcgtgatttc gtcgagggtg tacccgacgg ccagcttggc ggcgatcttg
     1981 gcgatgggaa atcccgtcgc tttcgacgcc agggcggagc ttctggagac gcgcggattc
     2041 atctcgatga cgaccatttc gccgttgacc gggttcaggc cgaactggat gttggctccg
     2101 ccggtgtcga cgccgatctc acgaatgatg cgcacggctg catcgcggag catttgatat
     2161 tctttgtcgg tcaaggtgag ggccggggcc accgtaatac tatcgccggt gtgcaccccc
     2221 atcgggtcga gattctcgat cgggcagatg atgaccacgt tgtccttcag gtcacgcatc
     2281 acttcgagtt cgaactcttt ccatccgatg acggactgct cgatgagtac ctggcggacc
     2341 gggctcatgg acaggcccca ttccacctgc gtccggaatt cttcaatgtt gtaggcgata
     2401 ttgccgccgg tgccgcccat ggtaaacgac ggtcgcacga tcgcggggaa gcggatccgt
     2461 tccagttcac gctcggcctc ggccagggag gttgccacac cgctgtccgg cacgcgcagg
     2521 ccgattttcc acatggcctg ccggaaggca tcgcgatcct cggccttatg gatggccgcg
     2581 atcgacgcgc cgatcaacct gacgccgtat ttttcgagga caccacgctt ggccagaccg
     2641 atggcggtgt tcaacgccgt ctgtccaccc atggtgggga ggagcgcatc gggccgttcg
     2701 cattcgatga ccttctccac cacgtccagc gtgatcggtt cgatatacgt gcggtccgca
     2761 aaatccgggt cggtcatgat cgtcgccgga ttgctgttga tcaggatcac gcggtagccc
     2821 tcttccttga gggccttgca ggcctgcgtg cctgaatagt cgaattcaca cgcctgcccg
     2881 atgacgatgg ggccggaacc gatcagaaga atggagcgga tgtctgtccg tcgtggcaca
     2941 attgacctca actcaagaag ttagccggtc ggcagaggcg cctcgggcat gggccccacc
     3001 ggtggccggt agggttggat cggtccggac gggatcgccg ggacctttcc tgcgggatga
     3061 tagtgtttca tggcgtccgg gatccaggcc tcgatctgat tgatacgagt cacatcggac
     3121 ggatgggtcg agagaaattc cggaatggcc tgctgcgagc ggaaacagag tttattgatc
     3181 atggcgcgcg gacagccgct catccgttcc cagaaggcga ccgcttcgcg cgggtcgtac
     3241 ccggcttcgg ccatcaagcg caggccgatg aaatccgctt ccgattcctg ccggcggtcg
     3301 aagggcaatg aaactccgac accgtagacg gtcatggccg ccatggccgc atccgggcga
     3361 cccgcggccg cgccggcgcc cagggccgcc aattgtccga tctgttcgag cacgctgcga
     3421 ctcatccgtt ccgctccgtg gcgctgcagg gcatgcgcga cctcatggcc catgacggtc
     3481 gccaacccgt cttcagtttt cgtgaccttc aaaattccgg tgaacaccgc caccttgccg
     3541 ccgggaagcg cgaaggcgtt gatcgtgcga tcgtcctgaa tgaccgcgaa ctcccactga
     3601 tactcaggct tattggccgc cttggcgatg cgatttccga cccgatgcac catttcgttg
     3661 agctccggat tgtcgctcaa gggagcctgc cggagcactt ctcgaaaggc cgagaggccc
     3721 atcgccattt ctttttcttc cgaaatatag atgaactgct cccgcgcggt gccgggcgcc
     3781 cgctggcacc cagcaagcga ttgcatcagg cctgccgccg cgccgagccc caccgtcgtc
     3841 gagaggagat aggcggcgcg gactcccagg gccaaggccg cccgtcttcc catcggggtg
     3901 tcgagtaggg tgtcgattga atgtcggcct gatggatgca tcatgtccac tccattcaat
     3961 cagcaccagg cgcgggatgc aactcaccgt ctcagggcat ccacagatac atgaactgcg
     4021 gagacccgcc tcgcacgacc gagagcacat cgaggttcgc cagactggcc aggctcggcg
     4081 ccggcgccac caagcgcgag tagacattgt tttggtattc gcccggtctg cgataggtca
     4141 ccactcgcgc ttccgtgagg ccggctttct ttttcgccag ctcgatggcg tcctcgagat
     4201 atccgatgtc atcgaccagc ccggaggctt tcgcctgctc gccggaatag atccggccgt
     4261 cggccagctt cctgatggtc tcgccgttca tgttcgggcg accgtcctgc acgacctgga
     4321 ggaatcgctg gtagaagccg tcgatgacgc cttggaatat cgcgcgttcc tccggcagca
     4381 tcgcccggaa gggcgagccc atatctttgc gcggtccgga ggtgacggcg ttggtctcca
     4441 cgccgacctt ttcgagcagc cctctggcat tgacggtcaa catgatcaca ccgatgctgc
     4501 cggtgacggt cgacggatga gcgagcacgg tatcggctgc agccgcaatg tagtacccgc
     4561 cggaggcgcc cacatccatg atggaggcca caatcggaat cttccggctg gtcttgaacg
     4621 acttgagttc gtgataaatg atgtcggagg ccgtgacggt gccgcccgga ctgttgatcc
     4681 ggagcacgac ggccttgatg cgctcatcct tcgtggcccg ctccagctct tctttcacgg
     4741 tcgcgagcat gccgggtgag gaataaaacc cgtccttatt ttcggagctg atcacacccg
     4801 acacgtccat cagcaggact ttgtctttgc ccgtcccgct gactttatgc tcttcaagcg
     4861 cgcccggtcc cggcggcaga ttgatcgtga cacaggcagc ctgcatcagg gccaggacaa
     4921 gcacggcaac cgcggtacag agatgccaag ggctgagggc ctgtccgact ttgcggttca
     4981 tgtcgaggcg acgaagatgc ggcctcgcat tcggctgcag atcgttcgcc acagcagaca
     5041 ggtcaacgtc gggcaggctc ctatgcataa tgtgtctcca tcaacgcgac aaattgttcg
     5101 aagagatagg ccgagtcgtg cgggcccggt gccgcttcag ggtgatactg gaccgagaaa
     5161 accgggcggt ccagacacat catcccttcc accgatccat cgttgagact ggtatgggtc
     5221 aactgcacgc gcccgaacgg cgtgtccaca atcggcatgg cctgtccttg cgccggtgcc
     5281 ccgatcggaa agcgcacggc gaagttatga ttctgtgagg tgatctcgac ctttcttgtc
     5341 cggaggtcga tgaccgggtg attggcgcca tgatggccga acttcagttt gtaggtggag
     5401 aagccaagcg ccaggccgag caattggtgg cctaaacaga tgccgaaaat cggtaaccgg
     5461 ccgatcagtt cccgaagcgc gtccatggca tagggcaccc cctccgggtc ccctgggccg
     5521 ttcgagagga acaatccctg aggattgagc gccagcacgt ccttcgccgg ggtggatgcg
     5581 ggcaccacgg tgacctcgca gccgacatcg acgagccgcc ggagaatgtt ctgtttgacg
     5641 ccgaaatcat aggccaccac acgccaggtt tttcgggctg cctgcgcggc gccgggctcg
     5701 ggcatcgtca gcttcggcgc ccagtcgccg gttccggctg tccaggtgta gcgtcgctcg
     5761 caggtcaccg tggcggccag atcccgtccg atgatgctcg gcgccttccg cgccttgtcg
     5821 gcgaggcgac gcggatcgag atcgatgtgg gaaatcacgc ccggctgggc gcccttctcc
     5881 cgcagatgtg tggtgagggc tctggtatcg atgccctcga tcgccacgat gttcgcggcc
     5941 tgcaaatatt cctgaagcgt agccttgctg cgccagttgc tcgccaggcg gctggactcc
     6001 ttgacgacga atccttccgc ccagatccgg gtggactcga tgtcttccgg tgtcacgccg
     6061 tagttgccga tgtgaggaca ggtcatggtc acgatctgtc cccggtagga gggatcggtc
     6121 aacacttcct gatagccggt catggccgtg ttgaacacga cttcgcctcc ggtctcccct
     6181 tctgcgccga gggcccgtcc ttcgaaccag gtgccgtcgg cgagcgcaag aatcgctttc
     6241 ttcaagatga ctccttatcg agggacccag gatcgtacgc gcacggtcag cagcgcgatt
     6301 ccgagtccga catttacgat atacaaggcc cgtaccggtt tgtgcagttg aaagaatgct
     6361 tcgaacgccg ctttccgcgc ttcctctcct ttggaggcaa aggcctgtgc ctgcagggcc
     6421 gccgcctggg gatgtagcac aaacgtgatg actcccgcgg tgaggagcat ggctccgagc
     6481 aacagccatt cggttctggt gatctcgcca tccgccaagc ctcgctggac cagccaggcc
     6541 cgccacagga ctcccgaggt caggattgcc gccgatccga gcaccagacg gttgtacccg
     6601 tcgaaggcgc gggtcagaaa gaatccgccg gtgtcctgcc cgccgaacgt attgaacacg
     6661 gccgggatga cggcggcaac gagcaccaag agcccaccga tccagaccgc cagggccagc
     6721 agctcacagg taatacaggc gatcagtcct tggcgcaccg tgtcgaatcc ctatgcctgt
     6781 cgctctgagc gatcgagttc gaacacgaca cgaccggaga cgatggtggt cgtcacccgt
     6841 cccttcacct tccagccggc gaatggtgtg ttgcggctct tggaacgaaa ccgtgacggg
     6901 tccacttgcc actccctgtt cggatcgacg atggcgacgt cggccggcgc gccgaccgcc
     6961 agagttccgg cattgagact gaacgctttg gccggcgcgg tcgcaagttt atctaccgcg
     7021 gattcgagcg tgagcacccc ttcatccacc agggccaacg tcagcgagag ggcggtctcc
     7081 aggcccacga tgccgaaggg cgcttcggtg aactcctgct gcttctcttg ggtcgcatgc
     7141 ggggcatggt cggtcgcgat cacgtcgatg gttccgtcgc ggagtccttc cttgatcgcc
     7201 tgaacgtcct gcatggtcct cagcggagga ttcatcttcg cgtgggtatt gtacccgcgt
     7261 gtcagttctt cggtcagggt gaagtgatgc gggcaggctt cagcggtcac cttgaggccg
     7321 cgcgccttgg cttcccgcac catacgcacc gagccggcgg tgctgatatg cgcgagatga
     7381 aggcgggcgc cggtcagttc agccagcgag acattgcggg ccaccatgac atcttccgcc
     7441 gcggagggaa tgcccggcag gccgagttcg gtcgagacca gcccttcgtt catacacccg
     7501 ccctcggaca ggtgcagatc ttcgcagtgg tccacgaccg gcacgtcgaa ggcgcgcgcg
     7561 tattccatgg cgcgtcgcat gacgaggctg ttcatcaccg gctttccgtc gtcggagatg
     7621 gcgacacaac cggcgcggcg cagatcgccg atttccgcca gttctttgcc ttccgatttt
     7681 tttgtgatgg cgccgattgg atagaggtgg gcgttacccg ctgccttcgc gcgctcgagc
     7741 atgaattcgg tgacggcctg attgtcgttg acgggattgg tgttgggcat cgcgcagacg
     7801 gtggtgaatc cacccgcgac ggccgcagcc gttcctgact ggatcgtctc tttatactcg
     7861 aaacccggtt cgcggaaatg cacgtggaga tccacgaacc ccggaagcac taattgtccc
     7921 ttggcttgaa tgactgtcgc gccggccggc gcagtaagtg ccggtccgac tgccgagatt
     7981 gttccgtttt cgatcaggac atccgccacg ccgttcacgc gtccgggatc gatcacatga
     8041 ccgccttgaa tcagtagtgt catcgttcac tcataaaaag aaaaaggtaa aaggattcat
     8101 tcagtcgctt cgtcccgctc agccggctcc cgacatgagg tacaaaatgc ccatgcgcac
     8161 ggccacgccg ttcgccacct gatcgagaat cacggacgag agactgtccg ccacgtcggg
     8221 cgcgatttcc accccgcgat tgatggggcc cggatgcatg acgatggcgc cggtctcggc
     8281 cagctttacg cgttcgctgg tcaatccata gtgtcgcgca tattcccgga tcgtcggaaa
     8341 caacgcgcgg ccctgtcgct ccagttggag ccgcaacatc atgatcacgt tgacgcctcg
     8401 cagcgcttcg tccaagttgt ggtacacctg cactccgagc tggctgatct gcagagggat
     8461 catggtcggc ggtccgacga cccgcacctc cgctccgagt ttcgtgaggg caaaaatgtt
     8521 cgagcgggcg acgcggctat gcgccacatc gccgacaatc gcgacgcgca atccggcgaa
     8581 gcccattcgc cggctgcgga tggtgtacag gtcgagcaga gcctgtgtcg gatgttcatg
     8641 ccacccgtca ccggcattga tgaccgaaga cttgacgccc cgcgccaggg tctctgcggc
     8701 gccggctgag gagtgccgca ggacgatgat atcggcctgc atggcttcga tgttgcgggc
     8761 cgtatccagg agcgtttccc ccttcaccac gctgctggaa gagggggaaa aattgatgac
     8821 atcggcgctg agacgcttgg cggccaattc aaacgacgtt ctggttctgg tgctcggctc
     8881 gaagaagagg ttgacgaccg tcttgcctcg taacgccggc acctttttga tctcccggcc
     8941 ggagacttcc ttgaaggaat ccgcggtttc caggatcaac gtgatgtcgt ccgccgacag
     9001 gtcggtcagg ctgagcaggt ctttccgttt gagccccacg acaatgtctc cggttacgcc
     9061 ctgaggatga cgacgcgatc ctcttccccg ttttcttcga gatggacctc aatggcctct
     9121 tctcgtgacg tgggaatatt tttgccgatg taattggctt tgatgggcag ttgccgatgg
     9181 ccgcgatcga ccagtaccgc cagctgaatt tccgccggtc gtcccaggtc gatcaggcta
     9241 tccatggcgg cgcgaatggt gcggccggtg aagagcacat cgtccacgag gaccaccttg
     9301 agatcggaaa ttttgaacgg caccgaggtc ttgcggagga tcggctgatc cttgcggagc
     9361 gacaggtcgt cacgatagag cgtgatgtcc aattcgccga tcgggatctg ggtgccctcg
     9421 atgtcgtgga tgcgccgcac gagacgatgc gccaggtgca cgccccccgt ccggatgccc
     9481 accaaggcca ggtccttgat gcccttattg cgttccagaa tttcatgcgc gatgcgggtc
     9541 acggcgcggg cgatatcgcc ggcgtccatc accactcgct cctgccggtt gccagttgtg
     9601 ggttcggttg tcataggtgg ttcagtcgat gggggcataa aaaaaccttc ccatcggttt
     9661 ggatgagaag gtgcgggttg tcggacggct ggctgccgtg tcgtgtggtc atgatctcct
     9721 tacccacctc acaggatgag cgttaaaggc gagccgatct taacgagttg cccctaccgt
     9781 gtcaagaatt ctgtgccccg agtggcctgt gttgcacgac gacgccttca ttgactccct
     9841 gcatgccatt tgttagcttc gtgccgttga gaggcggtcc gcctctctcg actatcatcc
     9901 agtcacccgg aatttatcag ctcccatcct gaaaggatcg cgcatgactg cctctgctgc
     9961 ctctaccgcc acgcctgacc ttctgaccgc tttggccaat aaggctacgc accttcgcat
    10021 ggacagtgtg aaggcgacga ctgaagccgg gagcggacat ccctccagct gttgttcggc
    10081 cgcagacatc gtcgcggtcc tgttcttttc ggtgatgcgg tacgacccga aaaacccgaa
    10141 attgcccaat agcgatcgat tcgtcctctc gaagggccat gcggcgcccc tgctgtatgc
    10201 ggcctgggcg gaagccgggt tgtttcccaa atcggaattg ctcaaattgc gcaccctcgg
    10261 gtcggatctc gaaggccatc cgactccccg cctttcattc gtggatatgg ccaccggttc
    10321 gttggggcag ggattgccgg ccggagtggg tctcgcgttc aatgccaaat caatcgacaa
    10381 gaccgactac cggacctatg tgctgatggg cgatggcgaa tccgtcgaag gctccgtctg
    10441 ggaagcggcg gaagtggcgc ggcacgcggc gttggataat ttgtgcgcga ttgtggatgt
    10501 gaaccggttg gggcaaagcg accccacgat gttgcagcac gacatggagg cctatcgtgc
    10561 ccgatgggcc gggttcggct ggcatgccat tgtggtggac ggtcacgata tcggcgccct
    10621 cgtggcagcg tttgaggaag cggcgcggac caaggggcgg ccgacggtgt tgttggcgaa
    10681 gaccttcaag gggcgcggca tctctttcat ggagaatcat cccgaatggc acggaaagcc
    10741 catgaagaag ggtgaagaaa cgcagaaggc cttggatgag ttgacgcggc aactcaagcc
    10801 cggctcgacg caaccgcaga ttccgatgcc gacagccgtc aaagctgctg cccctgccaa
    10861 ggggacgatg gcgcctccgc cctacaaact cggtgattca gcggcgacgc gggaagcatt
    10921 cggagccgcc ctcctggcgc tcggcgaggc caactcgcag gtggtcgcgc tggatgcgga
    10981 tgtgaagaac tccacctaca gcgacaagtt cggcaaacgg tttccggacc gcttcctgga
    11041 gaactttatc gcggagcaga acatgctggg cgctgcggcc ggtatcgccg cctgcggaaa
    11101 gattcccttt gtggcgacct ttgccgcgtt cttcacgcgg gcctacgact tcattcgtat
    11161 ggcggcgatc agccagtcga acatcaaact ggtggggacg cacgtcggtg tgagtatcgg
    11221 tgaagacggc ccctcgcaga tggggctcga agacctggcg atgatgtcgg cgcagcccgg
    11281 tgtgacggtg ctgtacccgt ccgacgcgct ctcgatgtac aaactggtgg aagcggcggc
    11341 cgctcataag ggcatggtgt atctccgcgc cggtcgcccc aagacgccgg tgatttatgg
    11401 tgtggacgag cagttccgga tcggaggctc caaggtcgtg aggcagagtg ccgccgacca
    11461 gctgacgatc gtggcggccg gtgtgacgtt gttcgaggct ttaaaagccc atgaccaact
    11521 gaaggcggcc ggcatcacga cccgtgtggt cgatctctac agcatcgtgc cggtggatcg
    11581 ggcgacgttg ctcgattgcg cgcgggcga
//
LOCUS       DAOQRM010000078        11335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_4904_length_11335_cov_17.934131, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000078 DAOQRM010000000
VERSION     DAOQRM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4904_length_11335_cov_17.934131"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PKK81_07790"
     CDS             <1..873
                     /locus_tag="PKK81_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNV32816.1"
                     /translation="DLCMVRHGGVHAMLTAYNQVPIPSFMYGTAWKKEATTQLVQQAV
                     ATGFTAIDTANQLIHYEEALVGEALVALAKQGTPRARLFLQTKFTPVNGQDHRTPYDA
                     QADLTTQVTQSFASSLAHLHTDYLDSYVLHGPYGRRGLSDADWEVWAAIESLYDAGKT
                     KMIGISNVTADQLTLLCARAAHKPMVVQNRCYAALGWDQDVRTVCRTHGIIYQGFSLL
                     TANRELFADPEVRAMAAKYGMGLAQLVFRFAMQAGMLPLTGTTDPQHMQEDLRSDQFA
                     IAPDDLQRLEIIGM"
     gene            942..>2017
                     /locus_tag="PKK81_07795"
     CDS             942..>2017
                     /locus_tag="PKK81_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV32817.1"
                     /translation="MESLHQLEVIIMLLAVVLALTTIAQKIRIPYPIFLVLGGLALGL
                     VPGLPAVTLHPDLVFLVFLPPILWAAAYFTSLREFRQNLRPISLLAVGLVLATSAGVA
                     FVAHALLPGIGWAEAIALGAIVSPPDAVSATAIGKRLRIPRRIVTILEGESLVNDATA
                     LVLYRAAVGAAMSGSFALGHTLFEFVFAALVGVVIGIGVAWLTRWALCATDDGFTQIG
                     VTLLAPYIAWVCGESVHASAVLACVAGGLYLRQSFSGAVSPATRLQARAVWDLLIFIL
                     NGVIFILIGLQLGALRDSVPPGQFGPVLIAGAWVSATAILVRLLWVPLAALIPRWLSA
                     KLRARDPMPPWSGLFLISWTGMRG"
     assembly_gap    2018..2117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2118..2713
                     /locus_tag="PKK81_07800"
     CDS             <2118..2713
                     /locus_tag="PKK81_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HNV32818.1"
                     /translation="MPPWSGLFLISWTGMRGIVTLAAALALPVTTSAGVPFPFRAEII
                     LISFTVILATLVLQGLSLPPIIRLMHLKEDGGLEQEEAMAREHAATVALTRLDQVVTE
                     NWVSLEQVERVRLRYLQRLERLTKASLAEETTLSSTTESVQRLQYETLTAERMALIDL
                     RDNGTISDEVLHRLEQELDVAALHLGVGERRPGRTHR"
     gene            3073..3651
                     /locus_tag="PKK81_07805"
     CDS             3073..3651
                     /locus_tag="PKK81_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32819.1"
                     /translation="MGWGPAHAEESGANPDAKTEEPHWVISIRGGVAQTTQEHVSNAV
                     ASLGGAGSLQFLYQFSPYFRFGLTVDGQQQAVDVKNGAGTFGDLLTVAVMPTIEMRPF
                     QTTTAIPYLSVGLGMNFNMFSTATRVLPLDYGNTFAARIAGGLDIPLTPHWSLNTELA
                     WRLNKGDWTQMGMTGRFDATSVLGLVGIRYMF"
     gene            3824..4294
                     /locus_tag="PKK81_07810"
     CDS             3824..4294
                     /locus_tag="PKK81_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV32820.1"
                     /translation="MSIRLTHARPSDAPLVADLVGELLQEIMTAIGAKAFGFSRVETE
                     ARARRWLTDGTYTVLLAYHDEKVAGFLALSESRALYAEGAFGTIPELYVRPAFRSCNV
                     GTGLVAEAKKLSRARGWTRLEVTTPPLPQFDRTLAFYERQGFSISGGRKMKLSL"
     assembly_gap    4296..4395
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4396..4865
                     /locus_tag="PKK81_07815"
     CDS             <4396..4865
                     /locus_tag="PKK81_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32821.1"
                     /translation="TTGIQHFRRAENETVSVIRPVVQEERTGCGIAAAAALAGVSYAR
                     AKAVAASLGISAQDPKLWSETASVRRLLTQFGLRPSRATKSFRSWTGLPDCALLAIKW
                     HLEQGRPFWHWVVFVRENERSYVVDSNPILKAPLRTDFWRMRPVWFLTVTMKR"
     gene            4869..6107
                     /locus_tag="PKK81_07820"
     CDS             4869..6107
                     /locus_tag="PKK81_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNV32822.1"
                     /translation="MKIAIIGGGPAGLMAAEAALAGGAQVALYDSMASVGRKFLLAGK
                     GGLNLTHSDPPDLFLSRYGERRAQVAPWLAEFGADAIRTWARGLGVETYVGSSGRLFP
                     TDMKAAPLLRAWLRRLRQAGLMIHVRHRWHGWDEQKALCFETPQGTTSVRADAVILAL
                     GGGSWPKLGSDGAWVPLLAGRSISIAPLRPANCGFDVGWTEHFRTKFAGHPVKTVGVV
                     AKSPTGAVMRRMGEFVITETGVEGGVIYAVASCLRDEIEATGRGTLRLDLAPDRELPR
                     LIKDLSQPRGKKTMATHLQRRAHIDGVKAGLLREIVPKEDFADPTRLAAAIKALPLTL
                     TAPRPLEEAISTAGGVTCEALDKRLMLKALPGVFCAGEMVDWEAPTGGYLLTACFASG
                     RTAGVGAVAWLKERAGRAKA"
     gene            6094..6654
                     /locus_tag="PKK81_07825"
     CDS             6094..6654
                     /locus_tag="PKK81_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV32823.1"
                     /translation="MPRHNTTNDGVVALIVPGIGNSGPGHWQTLWEERHPGWQRVQQR
                     DWDRPVCAEWLLGLDAAMARLSAPPVLIAHSMGCLLVAHWAKRSSLPVRAAFLVAVPD
                     PAGPMFPPAAQGFQPVPSEKLRFPSLVVTSSNDPFGSVAYARGCAADWGSDFVEVGAV
                     GHINADSGLGDWPAGLALFDGFLKSL"
     assembly_gap    6667..6766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6783..7565)
                     /locus_tag="PKK81_07830"
     CDS             complement(6783..7565)
                     /locus_tag="PKK81_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNV32824.1"
                     /translation="MADIRLRIAFLHLAPIPGALAKNRHLIAHAITKAACLGAAWIIT
                     PELAVSGYTFADTLGTDWIAPQPDPWMEKVRLFAARKRVAIFLSHPERDPQSGRLYNT
                     VFAIAPDGRLAGSHRKINALRVGSEAWSTPGTETTVFPMEPLGNVGMLICADAFSPGI
                     ANSLKAKGAQILVSSAAWAPGLHGPNGEWERCTKDTGLPLFVCNRTGADRTLDFRKAD
                     SVVAQDGRRLLSMSSERSAIFLIDWDLKAGTLASPEYRQILL"
     gene            7770..11114
                     /locus_tag="PKK81_07835"
     CDS             7770..11114
                     /locus_tag="PKK81_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HNV32825.1"
                     /translation="MDQSTTIPSARKRGRTALVLLLLVMLCIGGLLALPWMLNRPSIG
                     AALLQQFERRTGHALSVKAWHVRILPSIRVELLQTQLHAPGSTRPLLSADRLEITLQW
                     LPLLEGRVVAHDLVIDRPRLTVSRATDGTWSLGGTAPAASPGESDSLTSMVQMVRNLL
                     VIDGAITVVDESNSAPRVPVHIVVSQGTLSNEMMGRHAKVQLSGEIPQAADRAAFTFG
                     GFLIGNHESGGMQAEGDLRLHHIHVRQALSVWGGQDSIFDGLTGAAQLDAHVRVTAGT
                     DGYEMAADNWKAQLADLSIQGTATVSGRGADRPRYSATLSAAPVMVTRLMSHLPSAWI
                     PAQIRDRLVEYGVDGLVTLQTLSVSGEVGSGARPDLSGSIGLRNGRMTLHSRYPSIED
                     LSAGLVFDSSQVRVTALRATVGPLRLTGDDLLIRQWHGDPQVDLKISGAGSLAGLVEV
                     ARQIEDVPILRALLSRVQGATGDVEGVAHVVGNPDEKTGLSLVDVDLRLRHAGFRSAL
                     LPLDVREVQAHIHVSPTVVRIERLEGQVGPAAFDAGGELTWTGSEFSSDVTISMVADA
                     ANAWSWIANAVEVGPRPDVEGVVRMQATVTGRVDEPRFAGQIQLKSVGMRIPTILTKP
                     LHAPASIDFDTRLSGGSLLAIRHVGLVLPPVQIIGDGTLSLSESMAFAGNVSSGAIAL
                     NKLPAGITLGPMKAGTISTKLHMEGQIRDRASWRTSGEIRFDRGTIVLDALRDPIHEA
                     FVTLRFDQDKILIPRLAFHVGESDLRISGSIAQWAESPKARLVIESSQVDIAAFLPSP
                     QPSSESRRGRFDGKSWWSEGRVDAFFFADHLYYKKFLVTDLSGRVVWDHGLLTVERIS
                     GDTNEGHVGGQIKVRSTGRRVEQARSTFHASGIPIERVLSLVREEPALSGWLTTSGKL
                     QAEFERTGFTLDALTSRQPIQILIEDGRLYQVPVISALLSVMNLPAVLQGQVNLAKDG
                     LPLDRLKMVLSISNGVVQAKEFLLDSPVLKISGTGRYDIQADRFDMVLATSPLGSYSA
                     VLKRIPLFGHLLAGDRQGFDTAIFELKGSANNPDLRYLPTESLMTGLKGTAQLAFDIL
                     VNAVTLPQKAYSMIEEGITGDEDEEF"
     gene            complement(11138..>11335)
                     /locus_tag="PKK81_07840"
     CDS             complement(11138..>11335)
                     /locus_tag="PKK81_07840"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNV32826.1"
                     /translation="VETQLAEDRSGDLDGIVVATDGGTVTLTGTVPKAERKARAAELA
                     RQVKGVKRVKNDLEIRPDVSR"
BASE COUNT         1899 a         3152 c         3607 g         2377 t
ORIGIN      
        1 gatctgtgta tggtacggca tggaggggtt cacgccatgt tgactgccta caatcaggtc
       61 cctattccgt cgttcatgta cgggactgca tggaaaaaag aggcgacgac gcagcttgtg
      121 cagcaggccg tggccacggg gtttaccgcg atcgatacgg ctaatcagtt gatccattac
      181 gaggaagcat tggtcggaga ggcgttggtg gcgctggcga agcagggcac gccgcgggcc
      241 cggctctttc tgcaaaccaa gttcacgccg gtgaacggtc aggaccatcg cacgccttac
      301 gatgcgcagg ccgatcttac gacgcaggtg acacaatcgt tcgccagttc cctcgcccat
      361 ctccacaccg actatctcga ttcctatgtc ttgcacggcc cctatgggcg gcgtgggttg
      421 agcgatgccg attgggaggt ctgggccgcc atcgaatccc tgtacgacgc ggggaagacg
      481 aagatgatcg gcatcagcaa cgtgacggcg gatcaactca ccctgctttg tgcgcgggcc
      541 gctcataagc cgatggtggt gcagaatcgc tgttatgcgg cgctgggctg ggatcaggac
      601 gtgcggaccg tctgccggac tcacggtatc atctaccaag ggttctcgct gctgacggcc
      661 aaccgtgagc tgttcgccga tcctgaggta cgggccatgg ccgccaagta cggcatgggg
      721 ctggcgcaac tggtgttccg atttgccatg caggccggca tgttgccgct gactggtacg
      781 accgatccac aacacatgca ggaggatctg cgctccgacc aattcgcgat cgcgcccgac
      841 gatctccagc gactggaaat catcgggatg taaccggttg cctgtagccg cggcgattgg
      901 tccggcccgc gcgatcgcct cagaaacggt cttgtgcatt gatggaaagc ctgcatcaac
      961 tcgaagtgat catcatgttg cttgcggtgg tgctcgcgct gacgaccatc gcgcagaaga
     1021 tccgtatccc gtatccgatc tttctggtgt tgggaggtct ggcgctgggt ctggtgcccg
     1081 gcttgcccgc agtgacactc catcctgacc tcgtgttcct ggtcttcctg ccgccgatct
     1141 tatgggcggc cgcctatttc acgtccctgc gcgagtttcg ccagaacctc cggccgattt
     1201 cgcttctggc ggtcgggttg gtcctggcga cctccgccgg ggtggcgttt gtggcccatg
     1261 cgctcttgcc cggtatcggg tgggcggagg ccatcgcgct gggtgcgatc gtctcgccgc
     1321 ccgacgccgt gtctgcgacg gccatcggga aacggctgag aatcccgcgc cggatcgtca
     1381 cgatcctgga gggggaaagt ctggtcaacg acgccacggc gctggtgttg tatcgggcgg
     1441 ctgtgggcgc cgcaatgagc ggttcatttg cactcggcca tacgctgttc gagttcgtat
     1501 tcgccgcgct ggtgggagtg gtcatcggca tcggggtggc ctggctcacg cggtgggcgc
     1561 tctgcgcgac ggacgacggg tttacgcaga tcggcgtgac gttgctggcg ccgtacatcg
     1621 cctgggtgtg tggggagtcg gtgcatgcct ctgcggtcct ggcttgtgtg gcgggtgggc
     1681 tgtaccttcg tcagtcgttc agcggcgcgg tgtccccggc taccagattg caggcgcgtg
     1741 cggtgtggga tctcctgatc tttatcttga atggagtcat ttttatcctg atcggcctgc
     1801 agttgggcgc gttacgcgat tcggtgccgc cgggccagtt cggcccggtg ctcattgccg
     1861 gggcctgggt gagcgcgacc gctatcctgg ttcggcttct gtgggtgccg ttggccgctc
     1921 tcatcccgcg atggttgagc gcgaaattac gtgcacgtga tcccatgcca ccatggtcgg
     1981 gtctgttcct catttcctgg accggcatgc gcgggatnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnncca tgccaccatg gtcgggtctg ttcctcattt cctggaccgg
     2161 catgcgcggg atcgtgactc tggctgccgc attggcgttg ccggtgacaa ccagcgcagg
     2221 cgtgccgttt ccatttcgcg ctgaaattat tctgatcagc ttcaccgtga ttcttgcgac
     2281 cttggtcctg cagggcctat ccttgccgcc gatcatcagg ctgatgcatc tcaaagaaga
     2341 cggtgggctg gagcaggaag aggcgatggc gcgggaacat gcggcgacgg tggccttgac
     2401 tcggctggat caagtcgtca cagaaaactg ggtgtcgctg gagcaagtcg agcgggtgcg
     2461 cctgcggtat ctccaacggc tggagcggct caccaaggcc agtctggcgg aggagacgac
     2521 tttgagttcg accaccgaat ccgtccagcg cctgcaatat gaaacgttaa cggctgagcg
     2581 catggcgctg atcgatcttc gggataacgg cacgatcagc gacgaagtcc ttcaccgctt
     2641 agagcaggaa ctcgatgtgg ccgcgctcca cctgggcgtt ggagagcgac gtcccggcag
     2701 aacccacagg tgagcaaggt ctgtttggat gacttcctct tgatcaggaa caccgcgggc
     2761 gtaaaggcgt gacaaccggc tgcaactcct cttccctcgt cgacttcgcc acctcgttcc
     2821 ctcgttcata gaccggtggt tgcgcgactg ccactttcgt tccccccttc gtagtaacac
     2881 ttcagaggac tttcctccct tccggcgact catggatagt gcgccctcgg caataggcag
     2941 ggggttcact gaacacatgt gctcgtgccc gcttcatgag cccgggcacg gcgagtaagg
     3001 ggtgggagga tggctggcgc aaggcgttcg aaggtcgtga ttgttttgtt cctggttgga
     3061 gtgttgtgtg gcatggggtg gggacccgcc catgctgagg aaagcggtgc gaatccagac
     3121 gcgaaaacgg aagagccgca ttgggtgatc tctattcgag gaggcgtggc tcagacgacg
     3181 caggagcatg tctccaatgc ggtcgcatcg ttgggcggcg cgggcagcct ccaattcctc
     3241 tatcaatttt ctccgtattt tcgttttggt ctgaccgttg atggccagca gcaagcagtc
     3301 gatgtgaaga atggggcggg cacgttcggt gatctgctga ccgtcgccgt catgccgacg
     3361 atcgaaatgc gtccgtttca aaccaccacc gcgattccct acctctcagt cggccttggc
     3421 atgaatttca acatgttctc tacggccacg cgggtgcttc cgctggatta cggcaatacg
     3481 tttgcggcac gaatcgcagg cggactcgac atcccgttga cgcctcactg gagcctcaat
     3541 acggaactgg cgtggcggct caataaggga gactggacgc aaatgggtat gaccggccgg
     3601 ttcgatgcca cgtctgtcct tggcttagtc ggtattcgat atatgttctg acgctcgctc
     3661 cagcgatgcg ttccgcagga atgccggatg tgcttatccc gccttgttct gctgtccttc
     3721 atccgattct ctttcggcga cctccgctgt cggtgagtcc accgtaccga cagcggaagc
     3781 cagccggctt gacgcacgag cctgctgcgg gtaggttcgc gccatgtcga ttcgtctcac
     3841 tcatgcccga ccttccgatg cgccgctcgt ggccgatttg gtcggtgagc tgttgcagga
     3901 gattatgaca gccatcggcg cgaaggcgtt cgggttctcc cgggtcgaga ccgaagcgcg
     3961 ggcgcggcga tggctgacag acgggaccta tacggtgttg ttggcgtatc acgacgagaa
     4021 ggttgccggt tttctggccc tctcggagag tcgtgccctc tatgcagaag gcgcattcgg
     4081 aaccatcccg gaattgtatg tgcgtccggc attcaggtcc tgcaacgtgg gcaccggact
     4141 cgtggcagag gccaaaaagc tgtcccgcgc acggggatgg accaggctgg aggtcacgac
     4201 acctccgctc ccgcagttcg accggactct ggcgttttac gaacgacagg gattcagcat
     4261 ttccggcggg cggaaaatga aactgtctct gtgatnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnngaacg acagggattc agcatttccg gcgggcggaa aatgaaactg
     4441 tctctgtgat ccggcccgtt gttcaagaag agcgaaccgg ctgtggcatc gcggcagcgg
     4501 ccgcgttggc aggtgtgtcc tatgcgcgcg cgaaggccgt cgccgcttcg ttgggcatct
     4561 ctgctcaaga tccgaagctg tggtccgaaa cggcatcggt gcggcggctg ctgacacaat
     4621 tcggtctgcg tccctcgcgg gcgaccaaat cgtttcgctc ctggaccggt ttgccggatt
     4681 gcgcgttgtt ggccatcaag tggcatctgg agcagggacg accgttctgg cattgggtgg
     4741 tgttcgtgcg agagaacgag cggtcgtacg tggtagattc caacccgata ttgaaggctc
     4801 cccttcggac cgacttctgg cgtatgaggc cggtgtggtt tctgactgtg acgatgaaga
     4861 ggtaacaggt gaagatcgcg attatcggtg gtgggccggc cgggctcatg gccgctgagg
     4921 cggcattggc cggcggggcg caggtcgcgt tgtacgattc gatggcgtcc gtggggcgca
     4981 agtttctgtt ggccggaaaa ggcgggctga acctgactca ctccgatccg cccgatcttt
     5041 ttctcagccg gtatggcgag cgccgcgcgc aggtcgcgcc ctggttggcc gagttcgggg
     5101 ccgacgcgat tcgcacgtgg gcgcgcgggc ttggtgtcga gacgtatgtc ggctcctccg
     5161 gtcgtttgtt tccgacggat atgaaggctg caccgctgct gcgcgcctgg ttacggcggt
     5221 tgcgtcaggc gggattgatg attcatgtgc ggcatcgttg gcatggctgg gatgaacaga
     5281 aggccctctg tttcgaaacg ccgcaaggga cgacctccgt acgggcggat gcggtcatcc
     5341 tggcgttggg tggaggcagt tggcccaagc tcggctctga tggtgcgtgg gtgccattgc
     5401 tcgcggggcg ttccatttcg atcgctccgt tgcgaccggc gaactgcggg tttgatgtgg
     5461 ggtggaccga acacttccgg accaagttcg cggggcatcc ggtgaaaacg gtgggcgtgg
     5521 tggcgaaatc accgaccggc gccgtgatgc gccgcatggg ggagttcgtg atcactgaga
     5581 ccggtgttga aggcggtgtg atttatgccg tggcctcttg cctccgcgat gaaattgaag
     5641 ccaccggccg tggcacgctg cggttggatc tggcgccgga ccgtgaactg ccgcgtttga
     5701 tcaaggatct ctctcagccg cgcggaaaaa agacgatggc cacgcatttg cagcggcgcg
     5761 cccatatcga cggggtgaag gccgggctgc ttcgtgagat tgttcccaag gaagattttg
     5821 cagacccgac ccgtctggcg gccgcgatca aagccttgcc gctcaccctg acggcgcctc
     5881 gccctttgga ggaggcgatc agcacggcag gcggtgtgac ctgtgaggcg ctcgacaagc
     5941 ggttgatgct taaagcgttg ccgggagtgt tctgcgcagg agaaatggtg gattgggagg
     6001 cgccgacggg cgggtatctg ctcacggcct gtttcgccag cgggcgcaca gccggtgttg
     6061 gtgcggtcgc ctggctcaag gagcgtgcag ggcgtgccaa ggcataacac gaccaacgac
     6121 ggggttgtgg ccctgatcgt ccctggcatc ggcaattcgg gacccggcca ttggcaaacc
     6181 ttgtgggagg agcggcaccc tggctggcag cgagtgcagc agcgtgactg ggatcgtccg
     6241 gtctgtgcgg aatggctgct cgggcttgat gccgccatgg ctcgtctttc tgctccgcct
     6301 gtgctgatcg cgcatagcat gggttgtctg ttggtcgcgc actgggcgaa acgttcctcg
     6361 cttcctgttc gtgctgcgtt tctggttgca gtcccggatc ctgccggccc catgtttccg
     6421 cctgctgcac aaggctttca gccggtccct tcggagaaac ttcggtttcc cagtctggtg
     6481 gtgacgagca gcaacgatcc gttcggttcc gtcgcctatg cgcggggttg tgctgctgat
     6541 tggggcagtg actttgtgga ggttggtgca gtcggccaca ttaacgcgga cagcggtctc
     6601 ggtgactggc cggccgggct tgcgttattc gatggcttct tgaagtcgtt gtgacccctc
     6661 gccgtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttca ccgcgcatgt
     6781 cgctagagca ggatctgtcg atattccggc gaggcgagtg ttcctgcttt cagatcccag
     6841 tcgatgagga aaatggcaga ccgttccgag gacatcgaga ggagtcgccg cccgtcctga
     6901 gccaccacac tgtccgcctt cctgaaatcg agggtgcgat cggctccggt gcggttgcac
     6961 acgaagaggg gcagtccggt gtcttttgtg caacgttccc actcgccgtt cgggccgtgc
     7021 agtccaggag cccaggccgc cgacgagacc aggatctgcg cgcctttcgc tttcaagcta
     7081 ttggcaatgc ctggggagaa ggcgtcggcg cagatgagca tcccgacgtt cccgagtggc
     7141 tccatcggaa agaccgtcgt ttccgtgccg ggagtcgacc aggcttcgga cccgacgcgc
     7201 agcgcattga tcttgcgatg tgatcctgcc aggcgtccgt cgggggcgat ggcgaagacg
     7261 gtgttgtaga gtcggccgga ttgcgggtcc cgctcaggat gtgagaggaa gatggcgacg
     7321 cgcttcctgg cggcaaatag gcggaccttc tccatccagg gatccggctg cggggcgatc
     7381 cagtccgtgc cgagtgtatc ggcgaaggtg tagccgctga ccgcgagttc cggcgtgatg
     7441 atccaggcgg ctccgaggca cgcggctttc gtgatcgcgt gagcgatcag gtgacgattt
     7501 ttcgccaggg cgccgggtat cggagccaga tgcaggaacg cgattcgtaa tcggatgtcg
     7561 gccatcggtc acctgtacgg catacgcgtg cctgattgtt ctgatcaggc cggactgtag
     7621 catagcaagc cgttgatatc gactaagcat ggccggctcg atggacaggg cattctcatc
     7681 atacgtagag gctcgtcacg atcggctcat ctccgcggct tgtgcctgcc gcttctgttg
     7741 cgtacagtga accagaggcc aattgcacga tggaccaatc cacaacaatt ccttccgcca
     7801 ggaagagagg acgcaccgcg cttgtccttt tgttgctggt gatgctgtgt atcggtgggt
     7861 tgctggcgtt accctggatg ctcaatcgtc cgtcaatcgg ggcggcgttg ctgcagcagt
     7921 ttgaacggcg cacgggccac gcattgtcgg tcaaggcctg gcatgttcga atccttccgt
     7981 ccattcgggt tgaattgctg cagacgcagc tccacgcacc cggttccacc aggccgttat
     8041 tgagtgccga tcgcctggag attacgctcc aatggctgcc gctattggag ggacgagtcg
     8101 tcgcccatga tctggtcatc gatcggcctc gcctcacggt cagccgggcg accgacggca
     8161 cctggtctct gggcggaacc gctcctgcgg catcacccgg cgagtcggat tcgctcactt
     8221 cgatggtaca gatggtcagg aatctcctcg taatcgatgg cgcgatcacg gtcgtcgacg
     8281 agtcgaactc cgctcctcgc gttcccgttc acattgtggt gagccaggga actctgtcga
     8341 atgagatgat ggggcgtcac gcgaaggtgc agttatccgg cgagattccg caagcggcgg
     8401 acagagcggc ctttaccttc ggtggattcc tgatcggaaa tcatgaaagc ggcgggatgc
     8461 aggcggaggg agatctccga ttgcaccaca tccatgtacg acaggcccta tcggtatggg
     8521 gaggtcagga ctcgattttc gatggtctga cgggcgcggc gcaactcgat gcccacgtgc
     8581 gagtgaccgc cggaaccgat ggctatgaga tggccgcgga caactggaaa gcccagctcg
     8641 cggacctgtc gatacagggg accgcgacag tatccggccg gggagccgat cggcctcgct
     8701 attcggcaac cctgtccgcc gcgccggtca tggtcacgcg gttgatgagt catctcccct
     8761 ctgcctggat tcctgcacag attcgagacc ggcttgtgga gtacggtgtg gatgggctgg
     8821 tgacattgca gaccctgtct gtttccggcg aagtcggatc cggtgcgcga ccggatctca
     8881 gtggaagcat cggtctacga aacggccgta tgacgctcca ttcccggtat ccttccattg
     8941 aagacttgtc agccggtctt gtattcgata gctcacaggt ccgcgtcacg gcacttcgtg
     9001 cgactgtggg cccgttgcgc ctgacggggg acgatttgct catcaggcag tggcacggcg
     9061 atccgcaggt cgacctcaag atttccggag ccggttccct ggcaggattg gtggaggtcg
     9121 cacggcaaat cgaagacgtg ccgattctgc gcgccctatt gtcccgggtg cagggggcga
     9181 caggcgatgt tgaaggagtc gcccacgtgg tcgggaatcc ggatgagaag acaggccttt
     9241 cgctggtcga cgtcgatctc agactccgcc acgccggttt tcgcagcgcc ctgcttccgc
     9301 tcgatgtccg ggaggttcag gcgcacatcc atgtgtcgcc gacggtggtg cgcatcgaac
     9361 gcctggaagg ccaagtgggg cctgccgcgt tcgatgccgg aggggagctg acctggacag
     9421 gaagcgaatt ttcttccgat gtgacaataa gcatggttgc agacgccgct aatgcctggt
     9481 cgtggatcgc gaatgctgtc gaagtcggcc ccaggccgga tgtggagggg gtcgtccgca
     9541 tgcaggctac cgtaaccggc agggtcgatg aaccgcgttt cgcgggtcag attcaactca
     9601 agtccgtcgg aatgcgcatt cccaccatcc tcaccaagcc gctgcacgca cccgcctcca
     9661 tcgattttga tacccgcctg tccggcggca gcctcttggc gatccggcac gtcggactcg
     9721 tattgccgcc ggtgcaaatc atcggcgacg gaaccctcag cctctcggag agcatggcct
     9781 ttgctgggaa tgtctcgtcc ggtgcgatcg cgctgaataa attgccggcc ggcattacgc
     9841 ttgggccgat gaaggccggg acgatcagca cgaaactcca tatggaagga cagatacggg
     9901 accgggcgtc ctggcggacg tcgggagaaa ttcggttcga tcgcggcacg atcgttctgg
     9961 acgcgttgcg tgatccgatt cacgaagcct ttgtgaccct ccggttcgat caagacaaga
    10021 ttctgatccc gcgtttggcg tttcatgtgg gtgagagcga cctccggatc tccggttcca
    10081 ttgcgcaatg ggcggaatcg ccgaaggccc gtctggtgat tgaatcatct caagtcgaca
    10141 tcgccgcgtt ccttccctca cctcagccat catccgaatc acgtaggggc cgatttgacg
    10201 ggaagtcctg gtggtctgag ggcagggttg acgcgttctt cttcgccgat catctctatt
    10261 acaaaaaatt tctagtcacg gacttgtccg gccgggtcgt gtgggaccat ggtctgttga
    10321 cggtcgagcg gatcagcggc gataccaacg aggggcatgt cggcggtcag atcaaggtcc
    10381 ggtcgacagg acggagggtt gagcaggcca ggagcacgtt tcatgcgagc ggcattccga
    10441 tcgagcgcgt cttatctctc gtccgggagg aacctgcact gtcggggtgg ctcacgacgt
    10501 cgggcaagct gcaggcggaa ttcgagcgca cgggcttcac tctggatgcc ttgaccagcc
    10561 gtcagccgat tcagattctg atcgaggacg gacgactgta tcaggtgccc gtgatttccg
    10621 cattgctctc ggtcatgaac ctgccggcgg tactgcaggg gcaagtgaat ctcgctaagg
    10681 acgggttgcc gcttgatcgg ctcaagatgg tgctgtccat cagcaacggc gtcgtccagg
    10741 ccaaagaatt cctgctcgac agtcccgtcc tcaagatcag cgggacaggg cggtacgaca
    10801 tccaggcgga ccggttcgat atggtgctgg ccaccagccc gctcgggtcc tattccgcgg
    10861 tgctcaaacg cattccattg ttcggccacc tcctggccgg ggatcgacag ggattcgata
    10921 cggcaatctt tgaactgaag ggctcggcga acaatccgga cttgcggtat ctgcccacgg
    10981 aatcactaat gacggggcta aaggggacgg ctcaattggc ctttgacatt ctcgtcaatg
    11041 cggtgaccct gccgcaaaag gcctactcga tgatcgaaga gggaatcact ggagacgaag
    11101 acgaggagtt ttgagccgac gacaggatac cggagtctca ccgggagacg tcggggcgaa
    11161 tctcgagatc gttcttgacc cgcttcacac ctttgacctg ccgagccaat tcggccgccc
    11221 tcgcctttcg ctcagccttg ggtactgtcc ccgtcagggt gacggtgcca ccgtctgtgg
    11281 ctacgacgat accatcgaga tcgcctgagc gatcctccgc cagctgggtt tccac
//
LOCUS       DAOQRM010000079        10993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_5178_length_10993_cov_12.703694, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000079 DAOQRM010000000
VERSION     DAOQRM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5178_length_10993_cov_12.703694"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKK81_07845"
     CDS             <1..227
                     /locus_tag="PKK81_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNV32827.1"
                     /translation="LLVSLAFKAYVEHFGNYNAAYGSIAGVIVLMLWLYLTGVVILLG
                     GEINAQIQQAASSLRIRQEQAPQPVPAPAN"
     gene            379..>1190
                     /locus_tag="PKK81_07850"
     CDS             379..>1190
                     /locus_tag="PKK81_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNV32828.1"
                     /translation="MMWLGGILFCLWFGYYLRGVFTPVIIALLLAYLFNPLIRRAKDR
                     WHIPRPVTISCILFVSAVVILGLITWLGPLLAEQVQSFAERVPSYIQSIAQRYHVRLG
                     DFSEHLSGLATSLKDDPLSILRPVFSGTGQAFGVLGTVIGTTTDVVLAFILIPIYFFF
                     FAWHFDGTLDQIKRYIPTTYRSRVRHIVRRMDDAVSGFFRGRLAIALGSAVLYSLAWA
                     FTGIRYWFLLGLITGILTIIPYASLIGWPLAIALKYLDVLSSNAGGFDLMTIV"
     assembly_gap    1191..1290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1291..3003
                     /locus_tag="PKK81_07855"
     CDS             <1291..3003
                     /locus_tag="PKK81_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNV32829.1"
                     /translation="IAWGVDTVFSLPGDGINGIYEALRTRHDRIRLVQVRHEESAALA
                     ACGYAKFTRRLGVCLATSGPGGIHLLNGLYDAKSDGQPVLAITGHTFHDLIGTHYQQD
                     VSLDKLFSDVALYSERVMGPTHVRNVVDEAIKTAISRRTVAHITIPKDIQDWSAQEEG
                     SKANIPEHSGDRYSDPLPLPSQSLIERAAAVINAGSKPAILAGRGSLGAKSEILELAE
                     LLGAPIVKPLLGKAVVPDDHPLTTGGIGLLGTAPSQEVLETCDTLIIAGSSFPYLEFY
                     PKPGQARAVQIDLDASRIGLRYPVEVGLVGHCWDVLRALLPLVTHRSDRTFLTDAQGR
                     MAQWNTLMEERGSRMDMPLKPQVVVRAVNEFLADDAIVCCDTGTVTTWVARHIKMKDQ
                     MEFSASGTLATMGNGLPYSLGAGIAHPGRQIVCIAGDGGFTMLMGELATLVKYALPVK
                     IIVLKNNVLGMIKWEQLAFEGNPQYGVDLQPIDFAGFARACGATGFTVEDPRQVQDVL
                     RQAFSTPGPVVVEAVIDPLEAPLPGKITTDQAWQFAKALARGQDDRWEIIKTLVTGKI
                     REVV"
     gene            3046..3417
                     /locus_tag="PKK81_07860"
     CDS             3046..3417
                     /locus_tag="PKK81_07860"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HNV32830.1"
                     /translation="MEAIGRRRWAIAEGYLPAGSHGPAPQMTSHETVCILNTSDQHAD
                     VRITIFYADREPVGPYLVAVPARRTRHVRFNDLKDPAPIPTDTDYASVIESNVRIVVQ
                     HTRLDSRQAENALFSTMAFPA"
     gene            complement(3454..3612)
                     /locus_tag="PKK81_07865"
     CDS             complement(3454..3612)
                     /locus_tag="PKK81_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32831.1"
                     /translation="MIGERCMDIGESLGGYWFWAMRCIQCGDIVDEVILRNRHLPQER
                     PQEVVRAA"
     gene            complement(3863..>5731)
                     /gene="ligD"
                     /locus_tag="PKK81_07870"
     CDS             complement(3863..>5731)
                     /gene="ligD"
                     /locus_tag="PKK81_07870"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HNV32832.1"
                     /translation="HELKYDGYRMLCRVKDGTATLLTRNGHDWTAKLPHVAAAAADLR
                     INSAWLDGEVVALLPDGRVSFQRLQNAFDAQTDSHLVYVVFDLLYLDGYDLRPIPLIE
                     RKRLLSALLQDNSQSTLIRYSDHIAGQGEVAFAEACRSGMEGLIAKRADASYLAGRNR
                     NWVKVKCGRRQEFVIGGFTDPSGSRTVFGALLLGVFDENGRLRYAGRTGTGFSNRSLA
                     ELHARLKRLQRTKSPFVNPPGGSQAAGVHWVKPTLVAEVAFAEWTNEGQLRHASFQGL
                     REDKDAGSVVAERTSENASSEKPGQPNDRTKRTGRKNRAAQGDSKRASSAQATVVGGV
                     TLSHPDRVLFPEQAVTKLALARYYEAVRDWILPHVKDRPLTLVRCPEGYQKDCFYQKH
                     ANDQMPDVIGRVEVPADQGSACYTIAHSLEALLGLVQIGVLEIHTWGAKRDKLDRPDR
                     MILDLDPGPAVDWSSVIEGAQLLRTLLSELNLISFVKTTGGKGLHVVVPLQRVHTWDE
                     VKTFSKSLAEHLERMIPERFVATMSKAKRKGKIYVDYLRNAQGATAVAAYSTRARPGA
                     PVSVPLAWDELSIDLRSDSFTLVNLPDRLRRLSKDPWAEYGATKQRLTKTMRLRLT"
     assembly_gap    5732..5831
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5832..6267)
                     /locus_tag="PKK81_07875"
     CDS             complement(<5832..6267)
                     /locus_tag="PKK81_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HNV32833.1"
                     /translation="MGLQQYWKKRNFQRTPEPRGKNVPPDGRLRFVIQKHAASRLHYD
                     FRLELNGTLKSWAVPKGPSLDPAQKRLAVHVEDHPLEYAGFEGVIPEKQYGGGTVLLW
                     DRGYWEPIGDPGSSYRRGRLTFSLHGEKLHGIWNLVRMGGRKE"
     gene            complement(6301..6981)
                     /locus_tag="PKK81_07880"
     CDS             complement(6301..6981)
                     /locus_tag="PKK81_07880"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNV32834.1"
                     /translation="MLLSLATGCAQSAHQSGRDLLDRILPDHVQQQVDPSVSFAALKA
                     NPSAYQGNTIMVSGIVLKSRRVKDRTEIEVLQIPTGSGAVPTNDRTRSQGRFLAVKSG
                     EFLDPAVVDAGTPVTIVGEVGPAVTRAMDEGEYTYPVLEIKHLVDWDEVNPQIRFAPY
                     AYPYGRSMYAYPYSYWGPYGGLGGWYGGYPGYGYGYPYYGPFYGIGSGSSAPSGPPPA
                     SAPPQFKK"
     gene            complement(7025..7657)
                     /locus_tag="PKK81_07885"
     CDS             complement(7025..7657)
                     /locus_tag="PKK81_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNV32835.1"
                     /translation="MTRLGNIVAVSFLWFAAGMLHPHSAFAEWYVAGQFGANLADRLS
                     DIRGTGDLSGFRAPDFDLQNSFLYGGKVGTFPGNGWFGVELDAFHSTPHIKNLDDVPG
                     IHMRVTNVGVNLLVRYPGVTFQPYAGIGAGAAIARIGDSATTRGNTDVSSAVNVLLGM
                     RAFITPYVAVYTEYKYNQSTFEFNQAFGPASGFNGDYDAHILVTGLSYHF"
     assembly_gap    7758..7857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7902..9941)
                     /locus_tag="PKK81_07890"
     CDS             complement(7902..9941)
                     /locus_tag="PKK81_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNV32836.1"
                     /translation="MVQVDNMTLSVPLEQLQDNDALLSREWLVTNGLGGYASGTLLGA
                     PTRRYHGMFVPDLPSPWGRTVMIPRLDEEVVVEGESVLLSGIEFEDGRLESDLTKVIS
                     AFTREWQTPVWRCSFKGRRLEKRVIMPYGHNSVYVEYRLLEGDPVRLHLRPFVTFRML
                     DAPLHDARKPPFPLTVLDGRYEMSLCENAPSLKMCLRPHAGVFVADPLLSPGVSYRVD
                     RDRGAEHVESLASPGYFSADLMPGLPIAFVSSTEPWEHLEFTPEAIFDAEAQRLSKLV
                     AQLPDASQHDIERWLTLAADQFIVLPGSRMEEQALARASGDEARTVIAGYHWFTDWGR
                     DTMISLDGLTLCTGRYREARSILRTFANYMQDGLLPNLFPEGQRKALYHTVDATLWYF
                     HALDRYHRVTGDSETLLALRTVLKSVIEHHHNGTHFGIGVDRHDGLLRAGAEGYQLTW
                     MDAKVDGWVVTPRRGKPVEIQALWYNALRCMVEWGEMAGEETSEWALMATRARQSFNE
                     RFWRQEGGYLLDVIDGEQGDDPSFRPNQILAIALQHPILDESRWRSVVDAVEHKLLTP
                     VGLRSLSRDHRDYKPMYFGDLRARDAAYHQGTVWAWLIGPFIDAWLKVYKDASKSRDM
                     LGGFHSHLLENGIGTVSEIFDAEPPYHPRGCIAQAWSVAELLRVCRKTKQALEPQ"
     gene            complement(9944..>10993)
                     /locus_tag="PKK81_07895"
     CDS             complement(9944..>10993)
                     /locus_tag="PKK81_07895"
                     /inference="COORDINATES: protein motif:HMM:NF023367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HNV32837.1"
                     /translation="KAAGARSIILVAECEAQWVCTIRPTVERGWGLDAVWSEDFHHSA
                     RVAATGRSEGYYTDYRGTPQELISCVKRAFLYQGQRYQWQDGPRGTAVRAEPAEQFVF
                     FLQNHDQVANQLRGDRLHQKTGPGLHRALTALLLLAPETPMLFMGQEFGASAPFLFFA
                     DFPAGELARQIHQGRKQFLAQFPSYASAGAQAAVADPRDPAVFQRSKIDWSERERHAG
                     SVRLHRDLLRLRREDPVIAQQARDRLDGAVIGPAAFVLRYAGKQGDDRLLLINLGADC
                     DYRPAPEPLLAPPDGRTWHLLWSSDAPEYGGPGVIEPLTETGWVIPGESAALYSAKVK
                     NGRSVLEERGPIGTV"
BASE COUNT         2173 a         3347 c         3007 g         2166 t
ORIGIN      
        1 ggctcctggt gtccctggcg tttaaggcct acgtcgagca tttcggaaac tacaacgcag
       61 cctatggctc catcgcaggg gtgatcgtct taatgttatg gctctatctc accggggtgg
      121 tgatcctgct cggaggagaa atcaatgccc agattcaaca ggccgcctca tcgctccgca
      181 tccggcagga acaggcaccc caacctgtcc cggcccctgc gaattgaatg aacggctcac
      241 acagaatgga cacgatgttc tatgacaccc gtctatcctg acccgctgat aaaacaagag
      301 gtgcctttgc caccgggaga acaggtctcc cctgctcctg cggatcggca tttgtggcag
      361 atcacaccga tcagagacat gatgtggttg ggtgggattc tgttctgcct ctggttcggc
      421 tactatctgc gcggcgtgtt tacgccggtc atcatcgccc tgttgttggc atacctcttc
      481 aacccgctga tcaggcgagc gaaagaccgc tggcacattc cccgccccgt cacgatttcc
      541 tgcattcttt tcgtttcagc cgtggtgatc ctcggactga tcacatggct gggcccgctc
      601 ctggcggagc aggtgcagtc gtttgccgaa cgggtcccgt cgtatatcca aagcattgcc
      661 cagcgctacc atgtccgcct gggtgacttc tctgagcacc tctcggggct tgccacgagc
      721 ctcaaggacg atccactttc aatcctgaga ccggtcttct ccggaaccgg ccaggccttt
      781 ggtgtgctgg ggaccgttat cggcaccaca acggacgtcg tcctggcatt catcctgatt
      841 ccgatttatt tctttttctt tgcctggcac ttcgacggca cgctcgatca aatcaaacga
      901 tacatcccga cgacctatcg atcacgagtg cgtcacattg tgcgacgcat ggatgatgcg
      961 gtcagcggat tctttcgcgg ccgcctggcg atcgccctcg ggtcggcagt cctgtactca
     1021 ctcgcctggg ccttcaccgg tatacgctat tggtttcttc tcggtttgat cacgggcatc
     1081 ttgacgatca ttccctatgc ctcactgatc ggctggccgc ttgccattgc cctgaaatac
     1141 ctggacgtcc tctcgtccaa tgcgggcgga ttcgatctga tgacgatcgt nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcgcctggg gagtcgacac ggtcttcagc
     1321 ctgcccggtg acgggatcaa cggcatctat gaagccctcc gcacccggca cgatcgcatc
     1381 agactcgtcc aggtgcgcca tgaagagtcg gccgccctgg ctgcctgcgg atatgccaaa
     1441 ttcacacgcc ggttaggcgt ctgtctggcc acgtcgggtc cgggcggcat tcacttgctg
     1501 aatggattgt acgacgccaa gagcgacggt caaccggtgc tggcgattac cgggcacacc
     1561 ttccacgact tgatcgggac gcactatcaa caggacgtga gcctggacaa actgttcagc
     1621 gatgtcgccc tgtatagcga acgggtcatg gggccaacgc atgtccgcaa cgttgtcgat
     1681 gaggccatca agaccgccat ctcccgtcgc accgtggccc acatcaccat cccgaaggat
     1741 attcaggatt ggagcgcaca ggaggagggc tccaaggcca acatcccgga acacagcgga
     1801 gatcgctaca gcgatccctt gccgctcccc tcgcaatcgc tgattgagcg ggccgccgcc
     1861 gtcatcaatg ccgggtcgaa gcccgccatc ctggcaggac gcggcagcct gggtgcgaaa
     1921 tctgaaattc tcgaactcgc agagctactc ggggcaccga ttgtgaagcc gctgctggga
     1981 aaagccgtcg tgccggatga tcatcccttg accaccgggg gaatcggcct gctcggcacc
     2041 gcgccctcac aggaagtcct cgaaacgtgc gacaccctga tcatcgccgg cagcagtttc
     2101 ccttatctgg agttttatcc gaaacccggt caagccaggg ccgttcaaat cgacctcgat
     2161 gcgagccgca tcggccttcg atatccggtg gaggtgggat tggtcggaca ttgctgggat
     2221 gtcctccgcg cactgcttcc actcgtgacg cacagatccg accgcacatt cttgacggac
     2281 gctcaggggc ggatggcgca atggaacacg ttgatggagg agcgaggcag ccgcatggac
     2341 atgccattga agcctcaggt cgtggttcgc gccgtcaacg agttccttgc cgacgacgcc
     2401 atcgtctgtt gtgataccgg caccgtcacc acctgggtcg cgcgacacat caagatgaaa
     2461 gaccaaatgg agttttcggc ctccgggaca ctggccacga tgggcaacgg actgccttac
     2521 agtttgggag ccggcatcgc acaccccggg agacaaatcg tctgcatcgc gggcgacggg
     2581 ggattcacca tgttgatggg cgaactggcg acgctggtga aatatgcgct gccggtcaaa
     2641 attatcgtcc tgaagaacaa tgtactgggc atgattaaat gggaacagct ggcgtttgag
     2701 gggaacccgc aatacggcgt cgacctgcag ccgatcgact ttgccggctt tgcgagggct
     2761 tgtggcgcaa ccggcttcac ggtcgaagat ccccgccagg ttcaggacgt gctgcgacag
     2821 gcgttttcga caccgggccc ggtcgtcgtg gaagcggtga tcgacccact ggaagcgccg
     2881 ctaccgggaa aaatcaccac cgaccaggcc tggcagtttg ccaaggcgtt agcccgtggg
     2941 caggacgacc gctgggagat tatcaagacc ctggtgaccg ggaaaatcag ggaagtggtc
     3001 taacaatacc cacggcgacg gagcgggcta gcaaggagga tcgagatgga agcaatcggt
     3061 cgcagacgat gggcaattgc agaaggatac ctgcccgccg ggagtcatgg gccggcgccg
     3121 cagatgacga gtcatgaaac ggtctgcatt ctcaacacgt ccgaccagca cgccgacgtc
     3181 cggatcacga tcttctacgc ggaccgggaa ccggttggtc cctatctggt cgcggttcct
     3241 gcccgacgca ccagacacgt tcgattcaac gatctgaaag accccgcacc cattccaacg
     3301 gatacggatt atgccagcgt cattgagtcg aatgtgcgca tcgtcgtcca gcatacccgg
     3361 ctcgactcac gccaggcaga gaacgcattg ttcagcacga tggcgttccc cgcctgattc
     3421 gctgttaccg tcccgctcgc tccgttttta tcctcacgcc gctcgtacca cttcctgcgg
     3481 cctttcctgc ggcaggtgtc ggtttcgcag gatcacctcg tcgacgatgt cgccgcattg
     3541 aatgcaacgc atggcccaga accagtagcc gccgagactc tcgccgatgt ccatacaacg
     3601 ctcgccgatc atcaagcctc cgcaccgttc gcagcaggac gatgaacctc ggacagattc
     3661 ggcggtctgc ctgcttctat tgatgagtgg gatggtcatg ctggtggagt tcatacggct
     3721 ctcctttcat tgtgatcatt gacgggatcg gcgggcgctc ccaccgtttg tcatgctcac
     3781 caatatcagt ctgccctgaa tcactccact agcactcccc ccaggtcccg gttgaaagca
     3841 ccctggccac gatcctcatg cactaggtga gtctaagccg cattgttttc gtgagtcgtt
     3901 gtttcgtcgc accgtactct gcccacgggt ctttggacag tcggcggagt cgatccggca
     3961 gattgacgag cgtgaaactg tcggaccgca ggtcgatcga gagttcgtcc catgccagcg
     4021 gcactgacac cggcgcgccc ggcctggcgc gcgtggaata ggcggccacg gccgtcgcgc
     4081 cctgcgcatt gcgcaggtaa tcgacataga tctttccctt gcgcttggcc ttcgacatgg
     4141 tggccacgaa ccgttcggga atcatccgtt cgagatgctc cgcgagagat tttgagaacg
     4201 tcttgacctc atcccaggtg tgcacgcgct gcaacggcac gacgacatgc agacctttcc
     4261 cgccggtggt tttgacgaag gagatcaggt tcaattcgct caacaaggta cgaagtagtt
     4321 gggctccttc gatgacggac gaccagtcca cagcgggacc aggatccagg tcgaggatca
     4381 tccggtcggg acgatccagt ttatcccgct tcgcccccca ggtatgaatc tccagcacac
     4441 cgatctgaac cagtcccagc aacgcctcga gcgagtgggc gatcgtatag caggcactcc
     4501 cttgatctgc aggaacctcc actcttccga tcacatcagg catctgatca ttcgcatgtt
     4561 tctgataaaa acaatctttt tgataccctt caggacaacg cacgagggtc aacggacggt
     4621 ccttcacgtg cggaaggatc caatcgcgga cggcctcata gtacctggcg agcgccagct
     4681 tggtgacggc ttgctccgga aacagcacgc ggtccggatg actcagggtt actcccccga
     4741 ccacggtcgc ttgagcagat gaagcccttt ttgaatcccc ttgagcggcg cggttctttc
     4801 gcccagttct cttcgtgcga tcgttcggtt gtccgggctt ctcactcgaa gcgttctccg
     4861 atgtgcgctc ggcaacgacg gaaccagcgt ctttgtcctc acgcaagcct tggaaggagg
     4921 cgtgccgcaa ttgaccttca ttggtccact cggcaaatgc gacttcggcg accagcgtcg
     4981 gcttcaccca atgcactccc gcagcctgac tcccgcccgg tggattcacg aaaggtgatt
     5041 tcgttcgttg taatcgtttc aagcgagcgt gcagttccgc aagggaacgg tttgaaaagc
     5101 ccgtcccggt tcgtccggca tatcgcaatc gaccattctc atcgaacaca cccagcagca
     5161 acgcaccgaa cacggtccgc gatccggagg gatcggtgaa tccgccgatg acgaattcct
     5221 gccgccggcc gcatttcacc ttgacccaat ttcgatttcg ccctgcgaga taggacgcgt
     5281 cggcgcgttt ggcaatcagc ccctccatgc cgcttcgaca ggcctcggca aaggccacct
     5341 cgccctgccc ggcgatgtgg tcgctatatc ggatcagcgt ggactgtgag ttatcttgaa
     5401 ggagagctga caggagacgt ttgcgctcaa tcagggggat cggccgcaga tcgtacccat
     5461 cgagatagag cagatcgaag acgacataga ccaggtgact gtcggtctgt gcatcgaatg
     5521 cattttgtaa ccgttgaaag ctgacccgac catccggaag caacgcgacg acttcaccgt
     5581 caagccaggc gctgttgatc cggaggtctg ccgccgctgc ggcgacatgg ggcagcttcg
     5641 cggtccagtc atggccgttt ctagtcagca acgttgctgt gccgtctttc acccgacaga
     5701 gcattcgata gccgtcatac ttgagttcgt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn ncttcctttc gaccacccat gcgcaccaga ttccagatgc catgcaactt
     5881 ttccccgtgc aggctgaacg tgaggcgtcc acggcgataa ctggaaccgg ggtcaccgat
     5941 cggctcccag tagccccggt cccaaagcag caccgtgccg ccaccatact gtttctcggg
     6001 aatgaccccc tcgaacccgg catactccag cggatgatcc tccacatgca cagccaggcg
     6061 cttttgcgcc ggatccagac tgggcccttt cggaaccgcc caacttttca gtgtgccatt
     6121 cagttcgaga cgaaaatcat agtggagacg ggatgcggca tgcttttgga tgacgaaccg
     6181 cagccgaccg tcaggcggga cgttcttacc ccgaggctcc ggcgtcctct ggaagtttcg
     6241 cttcttccaa tattgctgaa gccccatggg attcctccgc ctgatgacgg cccctccagc
     6301 tcatttcttg aactgtggcg gcgcagatgc gggtggtggc ccgctggggg cggatgaacc
     6361 gctgccgatc ccgtagaacg gtccatagta gggataccca tatccgtacc caggataacc
     6421 gccataccaa cctcccaatc ctccgtatgg cccccagtag gaataggggt aggcatacat
     6481 ggagcgccca tagggatagg cgtagggagc gaaccgtatt tgtggattga cttcatccca
     6541 atccaccaga tgtttgatct caagcaccgg ataggtatac tccccttcat ccatcgctcg
     6601 cgtcacggca ggtcccacct caccgacgat cgtcaccggt gtgccggcat cgacgaccgc
     6661 gggatccaaa aactctccgc tcttgaccgc cagaaatcgg ccctgggacc tcgtgcgatc
     6721 gttggtcggc accgcgccgg agcccgtagg aatttgcagt acctcgatct cggtacgatc
     6781 cttcactcgc ctgctcttga gcacgatgcc gctgaccatg atggtattgc cttgataggc
     6841 ggatggattg gctttcaatg cggcgaacga gactgacggg tcgacttgtt gctgcacgtg
     6901 gtccggcaag attcgatcga gcagatctcg gcctgattgg tgagcgcttt gggcacagcc
     6961 ggtcgcgagc gataacagca tagccgccac gccggccaga agatgcggcc agaccggtcg
     7021 gtttctagaa atggtaggac aggccggtga ccaggatgtg cgcatcataa tctccgttaa
     7081 acccactcgc cggtccgaat gcctggttga actcgaaggt ggactgatta tatttgtact
     7141 cggtatacac cgccacgtaa ggggtgatga aggctctcat tcccagcagc acgttcaccg
     7201 ccgaagacac gtcggtattc ccacgcgtcg tcgccgaatc tccgatgcgc gcaatcgccg
     7261 cgccggcgcc gatcccagca tagggctgaa acgtcacccc tgggtaacgc accagcaaat
     7321 tcacccccac attcgtgacc cgcatatgaa ttccgggcac atcatccaga ttcttgatgt
     7381 gcggcgtgct atggaaggcg tcgagttcca ctccgaacca gccgttaccg ggaaacgtcc
     7441 ccacttttcc cccgtagaga aacgaattct gaagatcgaa atccggcgca cgaaatccgc
     7501 ttagatcgcc ggtcccgcga atgtcgctga gccggtcagc caggttcgcg ccgaactggc
     7561 ccgccacata ccattctgcg aaagccgaat gagggtgtag catgcccgcc gcaaaccaca
     7621 aaaagctcac cgccactata ttgcctaacc gcgtcataga tcgtggtctc tctggattta
     7681 tcgggcttga gctcaatcga gacaggcgca gaagccgact gtaccggtac ggaacagcaa
     7741 gtggtagctg gcgtttannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc
     7861 cgtcctgcat attcggcccg gcccacggga gatccaggtg ttcattgagg ctccagggcc
     7921 tgttttgttt ttctgcagac ccgcaaaagc tctgcgacgc tccaggcttg ggcgatgcac
     7981 ccgcgtggat ggtagggagg ttccgcatcg aaaatctcgc tcaccgtgcc gatgccgttc
     8041 tccagcaaat gggagtggaa acctcccaac atatctcgtg acttcgacgc atccttgtag
     8101 accttcaacc aggcgtcgat aaagggcccg atcagccacg cccagaccgt tccctgatga
     8161 taggcggcat ctctggcgcg aaggtctccg aagtacatcg gcttgtagtc gcgatggtcg
     8221 cgcgagagac tgcgcagccc gaccggcgtc agcaacttgt gttcgacggc atccactacc
     8281 gagcgccatc gcgattcgtc gaggatcggg tgctgtaggg caatcgcgag aatctgattc
     8341 gggcgaaaac tgggatcatc gccctgctcg ccgtcgatga catcgagcaa atagccaccc
     8401 tcctggcgcc agaacctctc attgaacgat tgccgcgccc tggtcgccat cagcgcccac
     8461 tcgctcgtct cctccccggc catctccccc cactccacca tgcaccggag cgcgttatac
     8521 caaagagctt gaatttcgac cggcttgccg cgccgagggg tcacgaccca cccgtccacc
     8581 ttcgcatcca tccaggtgag ctgatatccc tccgcgcccg cccgcaagag cccatcgtgc
     8641 cgatccacgc cgataccgaa atgggtgccg ttgtgatgat gttcgatgac ggatttcaaa
     8701 acggtccgca gggccagcag agtctcgctg tcgccggtca cccggtgata ccggtccagc
     8761 gcgtgaaaat accagagcgt cgcatccacc gtatggtaga gcgccttccg ctgtccctca
     8821 gggaacaaat tgggcaggag tccgtcctgc atatagttgg cgaaggtgcg aagaatcgac
     8881 cgcgcttctc ggtatcggcc ggtacagagc gtgagtccgt ctaaactgat catcgtgtcg
     8941 cgtccccagt cggtgaacca atgatagccg gcgatcaccg ttcgcgcttc atcgcctgac
     9001 gctcttgcaa gcgcttgctc ttccatgcgc gaacccggga ggacgataaa ctggtcggcc
     9061 gcaagcgtca gccatcgttc aatatcatgc tgacttgcat ccggcagctg tgccaccaac
     9121 ttactgagcc gttgcgcttc cgcgtcaaaa atcgcttcgg gagtgaactc gaggtgttcc
     9181 cacggctccg tgctggacac aaacgcgatg ggcagacccg gcatcaggtc cgcgctgaag
     9241 tagccgggac ttgcgaggct ttcgacatgt tctgccccgc gatcgcggtc cactcgatag
     9301 gacacaccgg gactcaacag cgggtcggct acaaagactc cagcgtgagg ccgcaggcac
     9361 attttgagcg acggggcatt ttcgcacaga gacatttcgt aacgcccgtc gagcaccgtg
     9421 agcggaaagg gaggcttcct cgcgtcatgg agcggcgcat ccagcatgcg aaaggtcacg
     9481 aacgggcgga gatgcaaccg gaccggatca ccctccagca accggtattc cacatagacc
     9541 gagttgtgac cgtacggcat gatgacgcgc ttttccagcc gcctgccctt gaatgagcag
     9601 cgccagacag gagtttgcca ctcgcgggtg aacgcactga tgaccttcgt caggtcgctc
     9661 tccaagcgtc catcttcaaa ctcgatgccg ctgaggagca cggactctcc ctccaccacg
     9721 acttcttcgt ccaggcgggg aatcatgact gtccgccccc aaggcgaggg aagatcgggc
     9781 acgaacatcc cgtggtaacg gcgagtcggg gcgccgagca atgtcccgga ggcatagccg
     9841 ccgagaccat tcgtcaccag ccattctctg gagagcaagg catcgttgtc ctgaagctgc
     9901 tccagcggca cagagagagt catattatcg acttgcacca tggttatacc gttcctatcg
     9961 gacctcgctc ctccaagact gacctcccgt tcttaacctt ggcggaatac aacgctgccg
    10021 attcaccagg aatgacccat ccggtttcgg tcaagggctc gatgactccg ggtcctccgt
    10081 attccggcgc atcgctcgac cagagcaggt gccaggttcg cccatcgggc ggggcaagca
    10141 atggttcagg agcgggacga tagtcgcaat ccgcccccag attgatgagc agcagccgat
    10201 catcgccttg tttaccggca taccgcagga cgaacgctgc cggtccgatc acggcgccat
    10261 ccagccggtc acgagcctgt tgcgcgatga ccggatcttc ccgccggagg cgaagcagat
    10321 ctcgatgcaa tcggacggac ccggcatgcc gctcgcgctc cgaccaatcg attttggaac
    10381 gctggaacac ggcagggtcg cgaggatcag cgacggccgc ctgagccccc gcggaggcat
    10441 aactcggaaa ctgagcgagg aactgctttc tcccttggtg gatctgccgg gccaattcgc
    10501 cagctggaaa gtcggcgaag aacaaaaagg gagcggatgc cccgaactcc tgccccatga
    10561 ataacatcgg tgtttcggga gccagcagga ggagcgccgt caaggcgcga tgcagaccgg
    10621 gaccggtctt ctggtgcaat cggtctcccc gcaactgatt ggccacctgg tcatggttct
    10681 gaagaaaaaa tacaaactgc tccgccggct cggcacgaac ggccgttcca cgcgggccat
    10741 cttgccattg atagcgttgt ccctggtaaa gaaacgcccg ctttacacag gaaatcagct
    10801 cctgcggcgt gccccgatag tcggtgtaat agccttcgct tcggcccgtc gcggccacgc
    10861 gcgcgctgtg gtgaaaatct tcactccaga ccgcatcgag cccccatccc cgttcgaccg
    10921 tcggtcgaat cgtgcagacc cactgcgcct cacattcggc gaccaggatg atcgatcgtg
    10981 ctcctgccgc ctt
//
LOCUS       DAOQRM010000080        10987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_5186_length_10987_cov_16.281101, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000080 DAOQRM010000000
VERSION     DAOQRM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5186_length_10987_cov_16.281101"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1076
                     /gene="mdh"
                     /locus_tag="PKK81_07900"
     CDS             132..1076
                     /gene="mdh"
                     /locus_tag="PKK81_07900"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNV32838.1"
                     /translation="MMGRPKITVVGAGNVGGTVAQRLAEKNAYDVVLVDIVPGIPQGK
                     ALDITQAGPVCGYSTRVVGTNGYDETAGSSIAVITSGIPRKPGMSRDELLATNAKIVK
                     TVVRELVSRSPNIILILVTNPLDAMVHVARQVSGLPKSRVLGMAGVLDTARLRSFVAE
                     ELNVPGTEVQAMVLGGHGDTMVPLVRQTTVAGKAITDRLSPERLAALIKRTQDGGAEI
                     VGLLKTGSAFYAPSASAVDMVEAIMNDQKRVMPCAMFCEGEYGLKDVIVGVPVTLGRS
                     GGESIVEYELTAEERTALQASADAVRDLCAVVDRLLTA"
     gene            1176..1763
                     /locus_tag="PKK81_07905"
     CDS             1176..1763
                     /locus_tag="PKK81_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNV32839.1"
                     /translation="MKFLEDPYQTLGAGFALAVGLIVVYLGMAGVGAGDADWFGLIVR
                     WIHFLAGITWIGLLYFFNLINAGFLKSLDGPTKNIVIPKLMPAALNWFRHGATVTVLA
                     GIVLYGYLYSKGGTGALALGIGGLLGIIMMGNVHGIIWPNQKKVIAAVAAAAQGTPAP
                     PEMAQWGKTALIASRINFLLSIPMLFFMGAGSHLK"
     gene            2017..3318
                     /gene="nuoF"
                     /locus_tag="PKK81_07910"
     CDS             2017..3318
                     /gene="nuoF"
                     /locus_tag="PKK81_07910"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNV32840.1"
                     /translation="MTLPQPRVLQKLEGAPWEIDPYLKTGGYEAWRKCLKELTPNDIV
                     GEIKKAGLRGRGGAGFPTGTKWDKVLNHRVQERYFVCNAGEHEPGTFKDRHLLKHIPH
                     QLIEGCLIASRTVNAKASYIYVNHEYAEEEANLRKAIAQARERGLLGKNILGTGVDLD
                     LEVYSGHGSYVAGEETAMLESMQGRPAMPRQKPPFYPTDFGLYGKPTLVNNVETLCNI
                     PLILKNGAAWFTQVGTEKCPGTMLFSLSGSVNRPGVYEMPMGVTIRDLIETCGGGVPN
                     GRKIKAVFPGGPAFSMVTADQLDLPMDFDSLKKAGTGLGSAGTIVIDDATCMVAATLK
                     YSNFFKNESCGQCPPCRMGTINLATLMDKIERGEGSQKDLDSLLQLCGFVKGTGYCTL
                     ITGAAVLVQSSLRLFQHEFEEHIRLQRCPFQPARTGAGANA"
     gene            3331..3687
                     /locus_tag="PKK81_07915"
     CDS             3331..3687
                     /locus_tag="PKK81_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNV32841.1"
                     /translation="MPRVSFLHPQGRSGEVESNLTLLEAAKTLGFELNHDCGGNASCT
                     TCRVEVQMGGDNLSEIDFDEQDLLDREALSEPWHRLGCQARVLGDVVVRVPETKWVQP
                     TSASSGEVGKRGTGLP"
     gene            3751..4071
                     /gene="erpA"
                     /locus_tag="PKK81_07920"
     CDS             3751..4071
                     /gene="erpA"
                     /locus_tag="PKK81_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNV32842.1"
                     /translation="MVTITQLAEQKIKELMTEEKDVVGLRIYVRGGGCHGYQYGMAFE
                     SKMADDDTVIEKGDVKVIMDSQSAPLLQGAEVDYVDSLQGSGFSIKNPQAKTTCGCGS
                     SFSA"
     gene            4204..4653
                     /locus_tag="PKK81_07925"
     CDS             4204..4653
                     /locus_tag="PKK81_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNV32843.1"
                     /translation="MSQATITRRYRFCAAHRLHTDQLSAEENWATFGKCNNPNGHGHN
                     YVVFVSVKGDIDPVTHQVLDVSRLDAVVERIVVQRFDHQDLNLDPEFATQTTTGENLV
                     LLIWDLLVDKLPAGRLVKVGVIETRDNYFEYSGSVESKRREEGVRHG"
     gene            4646..5281
                     /gene="folE"
                     /locus_tag="PKK81_07930"
     CDS             4646..5281
                     /gene="folE"
                     /locus_tag="PKK81_07930"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNV32844.1"
                     /translation="MAKVVSGARARRSRVSAAPTDEDPIEGLMTNLLLELGEDPDRNG
                     LLNTPKRVAKAMRFMTQGYRQDIDHLLNGALFPIEYDEMVIVKDIDFFSMCEHHLLPF
                     FGKCHVGYLPNKKVVGLSKIPRVVDAFSRRLQVQERLTLQIAETLKKKLNAHGVAVVM
                     EARHLCMMMRGVEKQNTVAVSSSMLGVFRTQQQTREEFLKLIRGSSVRDGN"
     gene            complement(5278..6081)
                     /locus_tag="PKK81_07935"
     CDS             complement(5278..6081)
                     /locus_tag="PKK81_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV32845.1"
                     /translation="MFCDTKGIRLAYDEQGTGLPLVFLHAFPLNRSMWTPQTTALSRQ
                     FRTIAVDLRGHGESDAPLWAFSLEDYADDVCALLDHLAIPQAVLVGLSMGGYVGFAFS
                     RKYGNRLKGLVLADTRAQADSPEGRTGRFNLAQTAYGKGADAVADIMLPKLLGATSLQ
                     QKPELVDYVRRTIRDTPVSGILVDLMAMANRPDSVAHLRALTCPTLVVVGQEDHTTPL
                     ADAQVMATEIPGARLAVIPAAGHLSNLEQPEVFNDLVRNFVEELRAAGD"
     gene            6234..7523
                     /locus_tag="PKK81_07940"
     CDS             6234..7523
                     /locus_tag="PKK81_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNV32846.1"
                     /translation="MSSHDSTGDLFAPAQTATRPGKVPLAERMRPRSFDDLVGQDEVV
                     GPGRPLRNAIERDQLSSVIFWGPPGCGKTTLAGLVAQHTESQFVPFSAVTGGIPELRE
                     IIKAAEHRRAMGRATTLFVDEIHRFNKAQQDAFLPHVERGTVVLIGATTENPSFELIA
                     PLLSRSIVVLLKPLTEESLGSILDRAVADSERGLGSLRITLHPAARERLIAFGNGDAR
                     SLLTTVEFVAGQAPVGPDGSRIIDERVLEAALLKKALRYDKSGEEHYNLVSAYIKSLR
                     DSDPDGALYWLARMLEGGENPRFIARRMVIFASEDIGNADPMALVMANAVAQAVEFVG
                     LPEAQINLAHGTTYLASRPKDNASYVGLQEAVRDARQHGNLGVPLHLRNAVTSLMKDI
                     GYGKGYRYVHDDPAAKTDQNHLPEPLRGTRYYRPRTP"
     gene            7586..7963
                     /locus_tag="PKK81_07945"
     CDS             7586..7963
                     /locus_tag="PKK81_07945"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV32847.1"
                     /translation="MARQSSSAESVSERRKYVRATLVGSALVSPQSGAKGFTAVLNNV
                     NKIGAGLHSKETLPIGAKITVSLAFLDPDRVEQQEKLNATVAWAKPWEKGILLGVVWD
                     DLVTKEKNRWLYYYLEETLKSTD"
     gene            complement(7960..9801)
                     /locus_tag="PKK81_07950"
     CDS             complement(7960..9801)
                     /locus_tag="PKK81_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV32848.1"
                     /translation="MLQLESVHKQFATKVLLEGATAHLRPGARVGLVGPNGAGKTTLF
                     RMILGEESPDKGTIRKRPRLRVGYLPQELETIVGKTVLDATHRDLYPEHEAERILAGL
                     GFSEADFSRPIENLSGGYRMRVALAHLLLSNPDVLMLDEPTNHLDKPTQRWFERFLLD
                     SNLTLLVISHDTKFLDGIATHIWELRDRTIQEFRGNYTKFQELRAARDAQIESAANRQ
                     SKEVARVQNFIDRFRYQANKAKQVQSRIKQLDKVKLIERQRDTKRVRFKFPLPSASGR
                     HVLELKGVAKSYGEKVIYRSLDFTVERGQRIALVGENGAGKSTLLKILAGVLPFEKGT
                     RQVGHGVTLHYFAQHQAESLDPDDTILESLAEVSAQAETNFLRGIAGAFLFSGDDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDMLTDALTDFPGTIIFIS
                     HDPTFLTRVATLIVEVDEGQAKNYLGDYEYFLWKKAQEFESIKESSAELAAAQAGKSD
                     GPTKAMASQVQQKSQGGERRDLNKTQARLEKQVSRAESEIASMETKVKARELELADPA
                     LYQELGRWSELQREQEGWKKELERLTARWESLSDELQGVREKLTAIG"
     gene            complement(9960..10035)
                     /locus_tag="PKK81_07955"
     tRNA            complement(9960..10035)
                     /locus_tag="PKK81_07955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10000..10002),aa:Ala,seq:ggc)
     gene            10395..>10987
                     /locus_tag="PKK81_07960"
     CDS             10395..>10987
                     /locus_tag="PKK81_07960"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNV32849.1"
                     /translation="MANLVIIGSQWGDEGKGKIVDILAKDADMVVRYQGGSNAGHTVI
                     NKRDTFIFHLIPSGILYRGTRCLIGNGVVVDPGALIEEMDHLQGQGVKIGKNFAVSDR
                     AHLILPYHKAIDKASEQSKGVRRIGTTGRGIGPSYGDKMARIGIRMGDLLNPPLFKQK
                     LEENLVDINWLLEQLHKVDCFELEKVYQQYMGYADRL"
BASE COUNT         2276 a         2905 c         3477 g         2329 t
ORIGIN      
        1 gataattttg tctgcacgat caatttcaag gtcggcggct ggacgggtca cgaacacgcg
       61 tgaagggtaa acggtgagtg taagggacct cttgcacttg acgcttcacg tttcacgctt
      121 cacgaggtgg gatgatggga cgaccaaaga ttacggtagt gggcgcgggg aatgtcggcg
      181 gaacggtcgc gcagcggctg gcggagaaaa atgcatacga tgtcgtactg gtcgacattg
      241 tgccgggcat cccacagggc aaggccttgg atattaccca ggccgggccg gtgtgcgggt
      301 acagcacccg cgtcgtcggt accaatgggt atgacgagac cgccgggtcc tccattgccg
      361 tcatcacgtc gggaattccc aggaaaccgg gcatgagccg ggacgaactc ctggcgacga
      421 acgccaaaat cgtgaaaacc gtggtgcggg aactggtatc gcgttcgccg aacatcatcc
      481 ttattttggt gacgaatccg ttggatgcca tggtgcatgt ggcgcgacag gtcagcggac
      541 tgccgaaatc acgagtcctc gggatggcgg gtgtcctgga tacggcgcgc ttgcggtcgt
      601 tcgtagccga agaattgaat gtgccgggca cggaggtgca agcgatggtg ttgggagggc
      661 acggggatac gatggtgccg ctggttcggc aaacgacggt ggccggaaag gcgattacgg
      721 atcgattgtc tccggagcgg ctggcagcct tgattaagcg cacacaggac ggcggggccg
      781 aaatcgtggg gctcctcaaa accggcagcg cgttttatgc gccgtccgcc tctgcggtgg
      841 atatggtcga agcgatcatg aacgaccaga agcgcgtgat gccctgcgcc atgttctgcg
      901 agggggaata cgggctgaag gatgtcatcg tcggtgtgcc cgtgaccttg gggcgcagcg
      961 gaggagaatc catcgttgaa tatgagctga ccgctgagga acgcaccgca ctgcaggcgt
     1021 ctgcggacgc ggtgcgggac ttatgcgcgg tggtcgaccg cctgctgact gcctagccgg
     1081 ctgactaggg tgtgtggagg cgggttgctt gacaaggctc agcggcctgc agtagagaac
     1141 ggggctggca ctaaaccgtt acagttgggg ggcgtatgaa atttttggaa gatccatatc
     1201 agacattggg cgcaggattt gcgctcgctg tcggattgat agtcgtctat ctgggtatgg
     1261 ctggagtggg ggcgggcgat gccgactggt tcggcttgat cgtgcgttgg atccatttcc
     1321 tggccggcat cacctggatc gggctgttgt acttctttaa cctcatcaat gccggctttc
     1381 tcaagagcct ggatgggccg acgaagaaca tcgtcattcc caagctgatg cctgccgcgc
     1441 tgaattggtt ccgccatggc gccacggtca ccgtcctcgc ggggatcgtg ctctacggct
     1501 acctctattc aaaaggtgga acgggggcgc ttgcgctggg gatcggcggt ttgctcggca
     1561 tcatcatgat ggggaatgtg cacgggatta tttggcccaa tcagaagaag gtcattgcgg
     1621 cggtggctgc cgctgcgcag ggtacgcccg ctccgccgga gatggctcag tggggaaaaa
     1681 cggccttgat tgcatctcgc atcaacttct tgctgtctat tcccatgttg ttcttcatgg
     1741 gagcggggag ccatttgaag taagccgtcg ggcgagcaga atcacgggga ttggtcatcc
     1801 gatgagggtg gccagtcccc tttttcttgg gccgatcgat gccgggtacc gtaatcgaat
     1861 gtcggcgagt ctttggactg attcgcggta ggcctatcag gcctaactga ttgagattca
     1921 ggcctaatca ggcgaacttg gcttgacggc ccccaaagag cagccgtata gtacattcct
     1981 gctaacctgg gggtgctacc cccgaaaggg aataacgtga ccctacctca accaagggta
     2041 ttgcagaagc ttgaaggggc tccctgggaa atcgacccct acctcaagac cggcgggtat
     2101 gaagcctggc ggaagtgcct gaaagagctg acccccaacg acatcgtcgg cgaaattaag
     2161 aaagccggat tgcgcgggcg tggcggtgcc ggctttccga ccggtacgaa gtgggacaag
     2221 gtcctcaacc atcgagttca ggagcgttac tttgtctgca atgccgggga acatgagccg
     2281 ggcaccttta aagatcgtca tctcctgaaa catatcccgc atcaactgat cgagggctgt
     2341 ctcattgcct ctcgaaccgt gaatgccaaa gcctcctata tttacgtcaa ccatgagtat
     2401 gcggaagaag aggcgaatct caggaaagcc atcgcccaag cccgggaacg tggcctattg
     2461 gggaagaata tcctcggaac tggcgtggac ctcgaccttg aagtctactc cggtcatggc
     2521 agttacgtag cgggtgaaga gacggccatg ttggagtcca tgcagggacg tccggccatg
     2581 ccgcgtcaga agccgccgtt ttaccccacc gatttcggac tgtatgggaa gccgaccctg
     2641 gtgaacaacg ttgaaacgtt gtgcaacatt cccctgattc ttaaaaacgg cgccgcatgg
     2701 ttcactcagg tggggacgga gaagtgtccc gggaccatgc tcttctctct tagcggatcg
     2761 gtgaatcggc ccggagtgta tgaaatgccg atgggcgtga cgatcaggga cctcatcgag
     2821 acgtgcggcg ggggtgtccc gaacgggcgc aagatcaagg cagtattccc aggcgggcct
     2881 gcgttctcaa tggtcacggc ggatcagctg gatctgccga tggatttcga ttcgctgaag
     2941 aaggccggga ccggactcgg ttccgccggg acgattgtga tcgatgatgc cacatgcatg
     3001 gtggccgcga ccctcaagta ctccaatttc ttcaagaatg aaagttgcgg ccaatgtcct
     3061 ccgtgccgga tgggcacgat caatctggcg acgttgatgg acaagatcga gcgcggggaa
     3121 gggagccaga aggacctcga ttcgttgctg caactgtgtg gatttgtgaa gggcaccggg
     3181 tattgcaccc ttatcaccgg tgccgcagtc ctggtgcaga gcagtttgcg attgttccag
     3241 catgaattcg aagagcatat tcgtttgcaa cgctgtccct ttcagccggc ccgcactggg
     3301 gccggtgcga acgcctagag gaatggtcgg atgccgcgtg tgagttttct tcatccccag
     3361 ggtcggagcg gtgaggttga atcaaatctc accctgttgg aggccgccaa gacgctgggg
     3421 tttgaactga atcacgattg cggcggcaat gcctcctgta ccacgtgccg ggttgaagtg
     3481 cagatggggg gggacaatct gtcggagatt gatttcgacg aacaggacct gttggaccgt
     3541 gaagccttgt cggagccttg gcatcggctg ggttgccagg caagagtgtt aggggatgta
     3601 gtggtgcgtg tgccggaaac caaatgggtg caacccacat cagcctcatc aggcgaagtt
     3661 ggaaaaagag gcacaggact tccgtaagct cccgtgagat ggtagagtat cgtaacgtgc
     3721 tgaacaggta gaccagaagg aggaccaacg atggtaacga ttacgcaatt ggccgagcag
     3781 aaaataaaag aactcatgac cgaggaaaaa gatgtggtcg gtctgcggat ttatgttcgc
     3841 ggcggaggat gccacggcta tcagtacggc atggccttcg aatccaaaat ggccgacgat
     3901 gatacggtca ttgaaaaagg cgacgtgaag gtcatcatgg actctcaaag cgcaccgctg
     3961 cttcagggcg cggaagtcga ttatgtcgac agcttgcagg ggtccggatt ctcgatcaag
     4021 aatccgcaag ccaagacgac ctgcgggtgc ggcagttctt tcagtgcgta agagccttcc
     4081 ggttgacccc caaccggatt gcgaatggtc cgttgagtag cagcaagccg ggagtggctc
     4141 cctttctagt ggggggccct cccggcttgt tcgttctgcc gatctttctg gagagttgtc
     4201 tcgatgtctc aggccaccat cacccggcgc tatcgtttct gcgctgccca tcggctgcat
     4261 accgatcagc tgtcggctga ggagaattgg gccactttcg ggaagtgcaa taatcccaac
     4321 gggcacggtc acaactatgt ggtgtttgtg tctgtgaagg gcgatatcga tccggtcacg
     4381 catcaggtgc tcgatgtgtc acggcttgat gccgtggtcg agcgaatcgt ggtgcaacgt
     4441 ttcgatcatc aggatttgaa cctggatccg gagttcgcga cccagactac gaccggagag
     4501 aatctggtgc tgctcatttg ggatttgttg gtggataagc ttcctgcggg acggctagta
     4561 aaggtcggtg tgattgaaac acgggataac tacttcgagt attccggctc cgtggagagc
     4621 aagcggagag aagagggagt gaggcatggc taaagtggtt tctggggccc gagccagacg
     4681 gtcgcgtgtc tctgcggcac cgaccgacga ggacccgatt gaagggctga tgacgaatct
     4741 tttgctggaa ttgggagagg accctgaccg taacggcctc ctcaatacgc cgaagcgggt
     4801 cgcgaaggcc atgcgcttta tgactcaagg atatcggcag gatatcgatc atctgctcaa
     4861 cggggctttg ttcccgatcg agtacgacga aatggtgatc gtgaaagaca tcgatttttt
     4921 tagcatgtgc gagcaccatt tactgccgtt ttttgggaag tgccatgtgg ggtacttgcc
     4981 gaacaagaaa gtcgtggggc tcagcaagat tcctcgggtg gtagatgcgt tcagccgacg
     5041 cctgcaggtt caggaacgat tgacgcttca gatcgccgaa acgctgaaga aaaagctgaa
     5101 tgcgcatggt gtggcggtgg tgatggaagc gcgacatctc tgcatgatga tgcggggcgt
     5161 cgaaaaacag aacaccgtgg ccgtgagcag ttccatgctg ggcgtgttcc gcacgcaaca
     5221 acagacccgc gaagagttct tgaagttgat tcgaggcagt agcgtcaggg acggaaacta
     5281 atcgcccgcc gcccgtagtt cctcgacgaa gtttctcacc agatcgttaa agacctcagg
     5341 ttgttcaaga ttactcaagt gcccggccgc agggatcacg gccagccggg caccgggtat
     5401 ctctgtcgcc atcacttgcg cgtcggcgag gggcgtggtg tggtcttcct ggccgactac
     5461 caccagagtt gggcaggtga gcgcgcggag atgggcgacg gaatcggggc ggttcgccat
     5521 ggccatcaga tcgacgagga tgccgctcac cggtgtgtcc cggattgtgc gccggacgta
     5581 atcaacgagt tcaggtttct gttgcagtga tgttgcgccc agtagtttcg ggagcatgat
     5641 gtccgccacg gcgtcggccc ctttcccgta ggcagtctgg gccagattga agcgtcctgt
     5701 tctcccctcc ggactatccg cctgcgctcg agtatcagcc agcaccaatc ccttcaggcg
     5761 gttcccgtat tttctggaaa acgcaaagcc gacatagccg cccatcgata atcccaccaa
     5821 cacagcctgg ggaatagcca agtggtcaag cagtgcgcag acatcatcgg cgtagtcttc
     5881 gagtgaaaat gcccacagcg gggcatccga ctcaccatgc ccgcgcagat ccacggcgat
     5941 ggtcctgaat tgtctggaca gcgcggttgt ctgtggcgtc cacatcgaac gattcagcgg
     6001 gaaggcgtgg agaaagacga gaggaagtcc tgtgccctgc tcatcgtagg ccaaccgaat
     6061 gcccttcgtg tcgcaaaaca tgagtccatc ctttgtgaaa agccgatgga tgggtggtag
     6121 cattgccgcg cgcgggaatc aaggggagga tcggcaggtg gggacgagag tcgtttgcga
     6181 tgattccctt gcgacagcta gaatgcaggg cagtattcag gtagagcacg accatgtcct
     6241 ctcatgattc cacaggcgat ctcttcgcgc cggctcagac ggccacccgt ccgggaaagg
     6301 tcccgctggc cgagcggatg cgtccgcgaa gcttcgatga cctcgtcggg caggatgagg
     6361 tggtggggcc gggacgcccg ctcagaaatg ccattgagcg ggatcaactc tcctccgtta
     6421 ttttctgggg accgccgggt tgcgggaaaa caaccctggc cgggctggtc gcgcagcaca
     6481 ccgaatccca gttcgtgccg ttttcagccg tcaccggcgg cattcccgag ttgcgcgaga
     6541 ttattaaggc tgcggagcat cgtcgggcga tgggtcgtgc gaccacgctg ttcgtcgacg
     6601 aaattcaccg gttcaataaa gcccagcagg atgccttttt gccgcatgtc gaacggggga
     6661 cggtggtgct catcggggcg accacagaaa atccttcctt cgaactgatc gcgcccttac
     6721 tctctcgctc catcgtggtg ctgttgaagc cgctgacaga ggagtcgctc ggttccattc
     6781 tggaccgggc agtggccgat tcggaacggg gactcgggtc gctccggatc acgttgcacc
     6841 ccgctgcccg cgagcggctg attgccttcg gcaacggtga tgccagaagc ctgttgacga
     6901 cagtggagtt tgtggccggc caggcgcctg tcgggccgga tggatcacgg atcatcgatg
     6961 aacgggtgct ggaggctgcg ctgctcaaaa aggcgcttcg ttacgacaag tcgggcgagg
     7021 agcactacaa cctggtgtcg gcgtatatca agagcctgcg cgactccgac ccggacggcg
     7081 ccctctattg gctggcccgt atgttggaag gtggggagaa tcctcgattc attgcccgac
     7141 ggatggtgat ttttgcgtca gaagatatcg gcaatgccga tccgatggcc ctggtgatgg
     7201 ccaacgcggt agcgcaggcg gtggagttcg tcgggctgcc ggaggctcaa atcaacctgg
     7261 cgcacgggac gacgtacctg gcctcgcggc ctaaggataa tgcctcctat gtgggcctgc
     7321 aggaggcggt gcgggatgcg cgccaacatg gcaatctcgg cgtgccgttg cacctgcgca
     7381 atgccgtgac gtctctcatg aaagacatcg ggtacgggaa ggggtatcgg tatgtgcatg
     7441 acgatccagc cgccaaaacc gatcagaatc acctcccgga gccgcttcgt ggcacacggt
     7501 attaccgccc cagaaccccc taattcgatg aagaaagggg ctggacaaac ctggacagac
     7561 ggttatactt agggccgagg tcatcatggc acgtcaatcc agttcagcgg agagtgtgag
     7621 cgagcgccgg aagtatgtcc gggccacgct cgtcgggtcg gccctggtgt cgccgcaaag
     7681 cggagccaag ggttttacgg cggtgctgaa taacgtgaat aaaatcggcg ccggccttca
     7741 ttccaaagag accctgccca tcggcgcaaa gattaccgtg tcgctggcct tcctggatcc
     7801 ggatcgcgtg gagcaacagg agaaactaaa cgccaccgtg gcttgggcca aaccgtggga
     7861 gaagggaata ttgctggggg tggtgtggga tgatctggtg acgaaagaga agaaccgctg
     7921 gttgtactac tatcttgaag agacgctgaa gtcgaccgat taaccgattg ccgtcagttt
     7981 ttcccgcacg ccctgcaatt catcggacaa cgattcccaa cgcgcagtga gtcgctccag
     8041 ttcttttttc caaccttcct gctctcgctg caattcgctc catcggccaa gttcctgata
     8101 gagggccggg tcggccagct caagctcacg cgctttcact ttggtttcca tcgaggcgat
     8161 ctcggactcc gcccgcgaca cctgcttttc aaggcgcgcc tgcgttttgt tcagatcccg
     8221 tcgttccccg ccctgagatt tttgttgcac ctgcgaggcc atggccttgg tggggccgtc
     8281 tgatttgccc gcttgggcgg cggcgagctc cgcgctgctt tccttgatcg attcgaactc
     8341 ctgcgccttc ttccacaaaa agtactcata gtcgccgaga taattcttcg cctggccttc
     8401 atccacttca acgatcagcg tggcgacgcg cgtcagaaac gtcggatcgt gggaaatgaa
     8461 gatgatcgtt cccggaaagt ccgtcagagc gtcggtcagc atgtccaccg aggccgggtc
     8521 caggtggttg gtcggttcgt cgagcagcaa cgtgtttgcc ggctccacca gcatgcgggc
     8581 caaggccacg cgattgcgct cgccgccgct gagggctttg atcggctttt tctggtcgtc
     8641 accggaaaac aggaaggcac cggcaatacc gcggagaaag ttcgtttcgg cctgcgcaga
     8701 gacctcggcc agcgactcca gaatcgtgtc gtccgggtcc aatgactccg cctgatgttg
     8761 ggcaaaatag tgaagcgtga ctccgtgccc gacctggcgc gtgccctttt cgaacggcag
     8821 caccccggcc aatattttca acagtgtgct tttcccggcg ccattctcac ccaccagcgc
     8881 gatccgttgc cctcgttcca cggtgaaatc cagcgaccgg taaatcacct tttcgccgta
     8941 gctcttcgcg acgcctttaa gttccagcac gtgccgaccg ctggccgagg gaagggggaa
     9001 tttgaagcgc acgcgtttgg tgtcgcgttg ccgctcgatt aacttgacct tatccaactg
     9061 cttgatgcgc gactgcacct gcttggcttt gttggcctga tagcgaaaac gatcgatgaa
     9121 gttctggacg cgagccactt ctttgctctg ccggttggcg gccgattcga tctgggcgtc
     9181 ccgcgccgcc cggagttcct ggaacttcgt atagttgccg cggaattcct gaatggtgcg
     9241 atcgcggagt tcccagatat gcgtggcgat gccgtcgagg aatttggtgt cgtgcgagat
     9301 gacgaggagc gtgagattcg agtcgaggag aaatcgctcg aaccaccgct gggtcggctt
     9361 gtccaagtgg ttcgtcggct cgtccagcat gagcacgtcg gggttcgaca gcagcaagtg
     9421 cgcgagcgcg acgcgcatgc ggtatccgcc ggaaagattc tcaatcggtc gcgaaaaatc
     9481 ggcttccgag aagcccaggc cggcgagaat ccgttcggct tcatgttccg gatataggtc
     9541 gcggtgcgtg gcatccagga cggtttttcc gacaatggtt tcgagttcct gcggaaggta
     9601 gccgacacgg aggcgcgggc gtttgcggat ggtgccttta tcgggagact cctcgcccag
     9661 aatcatccgg aagagcgtcg tttttccagc gccgttcggg cccacgaggc cgacacgggc
     9721 accgggacgc aggtgtgccg tggcaccttc gagcagcact ttggtagcaa attgtttatg
     9781 gacagattca agctgcagca tggatcagta agactcggtg taaggggtga aacgttcggt
     9841 gaggtacccg cggcagagtg tcagcgtccg tgtgtgcacc ggcggccggc caagcgtatg
     9901 actcgtagag ttcggatatc cccgcacaca ttcgaagcgt tggtcggaca agctttattt
     9961 ggtggagctg gccgggattg aaccggcgac ctcgtgaatg ccattcacgc gcgctcccaa
    10021 ctgcgccaca gccccacgca agcttgtctg tacatgtgca caggcacggg aaaaagaatt
    10081 aacgtgcaga cgtaactctg caatctagca tgggcggttt ttgtcagtca agaaaagcat
    10141 tggagtgcgg cgctgctggg tcctcatttt ttgctcgtgc agagagaggt agcaaaaaat
    10201 ctccgtggga tggtccggga agaggagggc gagcatctcg tttctgccaa cggatataag
    10261 gggcatcgct gaaaagcttg cttgacaaac cgcgaggcga atcctaccat gatgcccttg
    10321 cggctctgat cagtacgagg tcagggcttt tttttcgagt tcagcaaggg cagaaggagc
    10381 gtaggcagcg aacaatggcg aatctcgtca tcatcggctc ccaatggggc gacgaaggca
    10441 aagggaagat agtcgatatc ctcgcgaagg atgccgatat ggtcgtgcgg tatcagggcg
    10501 ggtccaatgc cggccatacg gtcatcaata agcgggatac gttcattttc catctcatcc
    10561 cctccggtat tctctatcgc ggcacccgct gcctgatcgg caacggcgtc gtggtcgatc
    10621 ccggggcgtt gattgaagaa atggatcatc tgcaggggca gggcgtgaag atcgggaaga
    10681 attttgcggt cagcgaccgg gcccatctga tcctgcctta ccataaggcc atcgataagg
    10741 cgtctgaaca gtcgaagggc gttcgtcgga tcggcaccac ggggcgcggg atcgggccgt
    10801 cgtatggcga caagatggcg cggatcggca ttcgcatggg cgatttgctc aatcccccgc
    10861 tcttcaaaca gaagctggaa gagaatctgg tcgatatcaa ttggctgctc gaacagttgc
    10921 acaaagtcga ctgtttcgaa ttggaaaagg tttaccagca gtacatgggg tatgccgatc
    10981 gtttgaa
//
LOCUS       DAOQRM010000081        10897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_5270_length_10897_cov_15.572588, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000081 DAOQRM010000000
VERSION     DAOQRM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5270_length_10897_cov_15.572588"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1074
                     /locus_tag="PKK81_07965"
     CDS             205..1074
                     /locus_tag="PKK81_07965"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV32850.1"
                     /translation="MSAELPKATDAASTDALEQHLIERIRKGAIELPLLPQVASRILS
                     MVYDPDAEAAKLAALIHQDQALAAHVIRIANSPAYMTRNPVVSLQHAVSMLGMNLMSE
                     LAFSASIKGSAFKVPGWDDEVKRLWQYSLASGAYAKEIARVRRFNVESAYLCGLLHGI
                     GKPVVLQTLVALAKEQNLTLTQELLHQLLEGYYIQVGLLVAEDWGLPPPVVESIGFHS
                     DYQYAKTAKQECMTTCLAGRLANHFLDPVAFDEAMVREHAVFADLNLYPKDIDALLAL
                     KDKVQAVVEAMPL"
     gene            1074..2492
                     /gene="sthA"
                     /locus_tag="PKK81_07970"
     CDS             1074..2492
                     /gene="sthA"
                     /locus_tag="PKK81_07970"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNV32851.1"
                     /translation="MPPNAYDIVVVGSGPAGQKAAIQGAKAGKKVVLIEQEQGIGGNC
                     VYRGTIPSKTLRETALQFERLKRSSEVFEGRLRLDVPMSVLLHRLDEVVKAHECYMAD
                     QLTRNSVTYRHGRARFLSPHEVELETIDGACQALRADTIVLATGSRPRSIPEIPVDHE
                     HVLDSDSILSMIYLPRSLTVLGGGVIACEYASTFALLGVEVTLIDRAQRPLSFMDAEI
                     VEVFQRSIERQGGRFYVGQTVKEVVWDGVSSVVARLANGMAVKSEKMLVALGRQPNIE
                     ELNLEAAGLTLDEKGRIPVNEYGQTPVPHIFAAGDMLGRPPALASQAMEDGRRAVSHA
                     LGLPVGDSLNQVPIGIYTIPEIASIGLDEEQAAARYRGPLVGRARFTEIAKGQITGSC
                     DGLLKLIADPSGERLLGVQIVGEHATELIHLGQMALQDGATIDRFIDSIFSFPTFAEG
                     YRVAALDILGQRRKRQSTTQAA"
     gene            2590..4293
                     /locus_tag="PKK81_07975"
     CDS             2590..4293
                     /locus_tag="PKK81_07975"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32852.1"
                     /translation="MNRNERRRDEKLHGKQAGGADAPAALLREAQLHHQAGRLDEAER
                     GYRSLLERAPTQPDALHGLGLLTYRRGNLKDALGWLAKACAAGPRNPIYWFNHGVVLQ
                     RAGRTTDAVEAYGQAIHWNPRYIEARTNLGNAYKELGRLSDAQAAYEQVLTLNPDHAE
                     AHNNLGVVLKEQGRLDEAAESYRRAIALKPTHAEAQNNLGLVLLEQGRLDDAIRCFER
                     ALQIVPGYGTALYNLGIAWIWREDIPRALRCFAETAQAKHAHGRPVAETTVFRSRLKH
                     DAEQLEYLRESGLLGDEWNPYHEALTRLCEKLEHSATGATGSSNRVPLSPADMQPIAA
                     SYNRLIHIAPCEAIEGGALAADLDSAAVEARYLATNPEVTYIDGLLADEALQRLRRFC
                     WASTIWKKDYENGYIGAFLGDGFASPLLLQIAEELRRRFPRIFGTHRLTQAWAFKHDS
                     ARRGLNIHADAAAVNVNFWITPDEANLNPDSGGLVVWDKEAPRDWDFKTYNSDKARGK
                     IYEWLNAQGAKEIKIPYRANRAVVFNSDLFHETDDIAFKEGFTNRRINITLLYGHRHR
                     P"
     gene            complement(4259..6385)
                     /locus_tag="PKK81_07980"
     CDS             complement(4259..6385)
                     /locus_tag="PKK81_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV32853.1"
                     /translation="MTQTRPTILIADDDPLSRLFVRNALEPAGMTVAEATGGQDALVK
                     FEALAPDLVVLDIMMPDMDGYLTCSRLRTLPRGKRVPILILTGLDDANSIAQAYQHGA
                     TDFITKPVNATILCHHVRYMLRTNNVLHALIRSESRLELAQRIARIGNWDWNPKTNRL
                     SMSNELCRLLGVRPQDFGGTFEAFLNLIHPDDRPVVTGALERLVSQHTPCDIDHRVVL
                     PNGTDFIIHLQAEGVREEETDELTVIGTAQDITERKQAERAIHQLAYYDSLTGLANRV
                     LFKDRLSNALSYAERYHQHLATLFIDLDRFKVINDTLGHTVGDRLLTHVAERLSESVR
                     QSDSVGRHADHEPTHALARLGGDEFTILLTALPTPEDAGRVARRILEALAHPLSIDGH
                     EIFISASIGISIFPSDGATVEALLKNADTAMYHAKEQGRNNCQFYSSGLNAAAAERLD
                     LENELRRALEREEFVVFYQPKLNIHSRKILGAEALVRWKHPKRGLVPPGVFLNAAIDT
                     GLIRPMDEWVLREACRQVKAWETAGLPPITVSANVSNSLFHGRTLPATVADALRDSGL
                     NPSQLELELTESIAMRDVEASVTMLEGLRTMGVRLSIDDFGTGYSSLSYLQRFPLSRL
                     KIDQSFVRDLLTNENNVKITRAIIAMAHSLNLSVLAEGVETEGQLARLREEGCDEVQG
                     YLFSRPVCAEDFEKLLRGDADARTAA"
     gene            complement(6615..7328)
                     /locus_tag="PKK81_07985"
     CDS             complement(6615..7328)
                     /locus_tag="PKK81_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32854.1"
                     /translation="MAHASRLPRTWLPAVFISVIVSACCAAALYVLLAQHDRSLVSQR
                     NAAAIMAAEQSARAMARTVAWFSESLPGESLGTVQQTLEQHARQANLLDAAVITTDNM
                     IVAASNPAIIGSPMQDGAWPAARNSQSSSITHGVDKGRPVMIVIEPFRQDNRIAGWIR
                     LVVATPPEAAALRSEDDLGRDVALVIGPLLLVMVILLTLTMRGLMSRVRALLAGILME
                     AGEQRFVPAGNPVETPERG"
     gene            complement(7345..7710)
                     /locus_tag="PKK81_07990"
     CDS             complement(7345..7710)
                     /locus_tag="PKK81_07990"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV32855.1"
                     /translation="MDQRHHPRFSVHFRSSFSSVNRVGGEGNVVDLSLRGCGILSATA
                     VHPGTTLELRIQPAGDAAALTIQQAVVRWCRDGRIGIEFVSLQPDEWTRLQGIVKELT
                     RQPYERANDRQDGPGTSRP"
     gene            complement(7734..9185)
                     /locus_tag="PKK81_07995"
     CDS             complement(7734..9185)
                     /locus_tag="PKK81_07995"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNV32856.1"
                     /translation="MKLNTDMQVVKINDYLWEIPPSEKPGMLVPARIYASAAILGAMD
                     RGVFDQVTNVACLPGIHRYALCMPDGHWGYGFPIGGVAAFNPEDGVISPGGVGYDINC
                     GMRLIRTDLSLSEVQPKLELLMTELFRRVPAGVGSRGFVNLSRHDFRDVMRQGAGWCI
                     SKGYGWEEDLERIEERGCLEGADPTHVTDYAIERGINQLGTLGSGNHYLEIQVLSDKG
                     IYDRATAAAMGLTGRDQVVVMVHCGSRGFGHQVASDYLKVFEKAMRRYGISVKDQQLA
                     CAPFRSPEGQDYFGAMNCAANTAFANRQVITHQIREAFEAVFGESARRLGMQLIYDVA
                     HNIAKVERYRDGEWLVHRKGATRAFGPGSPELPACYRGIGQPVICGGSMETGSYLLVG
                     TDRAMQETFGSTMHGSGRTMSRAQAKRTVRGEQLMRDMKQHGILVKAVSMSGLAEEAG
                     FAYKNISDVVETVDHAGITKKVAELKPIGNIKG"
     gene            complement(9352..9792)
                     /locus_tag="PKK81_08000"
     CDS             complement(9352..9792)
                     /locus_tag="PKK81_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNV32857.1"
                     /translation="MPGSFRFLDDIALADMAFDAEGESLSALFEAATQALIESLADPV
                     TIAETWRHSLDMEEADIETLLFEWLGRLVYLKDAQGVVFHRATLSLNQNDAHTAWHLH
                     AELTGAPVDPATQDLRSDVKGVTKHLYAVTQEGTSWKARVVLDV"
     gene            complement(9798..10772)
                     /locus_tag="PKK81_08005"
     CDS             complement(9798..10772)
                     /locus_tag="PKK81_08005"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNV32858.1"
                     /translation="MRLTFTIQRFNPETDQQPHREDYRLDIGRGMTVLEALIRIKNEL
                     DGRLALRYSCRSAICGSCAMQINGTEKLACRTSIRKELERHGRISIEPLPNLPVIKDL
                     VVDMSPFWEKIRAVTPWLTPETHPTKRYGSSGQLRLLPESYQFHNVDACIMCGACVAA
                     CTSHEVSRGFLGPAALAKSARFVADPREPAGAKQARLTALQESNGIWDCTRCNMCVQV
                     CPKDVQPMEAIIRLRRSSLRHGLTSAEGARHITGFVDLVRHEGRLNEALMPLKVLGFN
                     LRRVLDVMPLGVRMWLKGKVPLPFGHTIPGIEQVRAIFAAARRRAPRV"
BASE COUNT         2108 a         3217 c         3417 g         2155 t
ORIGIN      
        1 ttaacccgcg ttattcatga cggttcgtcg cgaaattgcc gagtcacgtg gcgagcccgc
       61 cggccaaagg cccgtcgcag cagtgaatcg gcgattgcag cagaagcgct catgaataat
      121 gcgggttaag gttttgggaa gaatgagccg aaaaggatct acaaggcaga caatcccgca
      181 accttgacgt gtggtgtaag ccctatgagc gctgaacttc ccaaagcgac ggacgcggcc
      241 tctacggacg cgttggaaca gcatctgatc gagcgaatcc ggaagggagc gatcgaactc
      301 cctctcttgc ctcaagtagc ctccagaatt ttgagtatgg tctacgatcc ggatgcggag
      361 gcggccaagc ttgccgcgct cattcatcag gaccaggccc tcgccgccca tgtcatccgg
      421 attgccaact ctccggctta catgacccgc aatcctgtgg tgtcgttaca acatgccgtc
      481 tcgatgctgg gcatgaactt gatgtcggaa ttggcgttct ccgcctccat caaaggcagt
      541 gccttcaagg tgccggggtg ggatgatgaa gtcaagcgcc tctggcagta ttcactggca
      601 agcggcgcct acgccaaaga aattgcccgc gtccgccggt tcaacgttga aagcgcctat
      661 ctctgcgggc tcctgcacgg gatcggcaaa ccggtggtgt tgcagacgct ggtcgcgttg
      721 gccaaggagc agaatctcac cttgacgcag gagttgttgc atcaactgct ggaaggctac
      781 tacatccagg tgggcttgct cgtcgccgaa gactgggggc tcccgccgcc tgtcgtggaa
      841 tcgatcggct ttcacagcga ttaccagtat gccaagacgg ccaagcagga gtgcatgaca
      901 acttgtctgg ccgggcgatt ggccaaccat tttctcgatc ccgtcgcatt cgatgaggcg
      961 atggtccgtg agcatgcggt ctttgccgat ctgaacctct atcccaaaga tatcgatgcg
     1021 ctgctggcct tgaaagacaa ggtgcaagcc gtcgtggagg cgatgccgtt gtgatgcccc
     1081 ccaatgccta tgacatcgtg gtggtgggga gcggtccggc cggccagaag gccgccattc
     1141 aaggcgccaa ggccggcaag aaagtcgtcc tgatcgaaca ggaacaaggc atcggcggca
     1201 actgtgtgta ccggggcacg attcccagta agacgctgcg cgagacggca ttgcagttcg
     1261 agcgtctcaa acggtcgagt gaagtgttcg aaggacggct gcggctcgat gtgcccatgt
     1321 ccgtgctgct gcaccgcctg gatgaggtgg tgaaggcgca cgagtgttac atggccgacc
     1381 aactcacccg caacagcgtg acctatcgac acggacgcgc gcgatttctc tcgccgcatg
     1441 aagtggaatt ggagaccatc gacggcgcct gccaggccct gcgagccgac acgatcgtgc
     1501 tggccacggg gtcccggccg cgttcgattc ctgaaattcc tgtcgatcac gaacatgtgc
     1561 tggacagcga ttcgatcctg tcgatgatct acctgccccg ctcgttgacg gtcttgggcg
     1621 gaggagtcat tgcctgcgaa tatgcctcga cgtttgcgct gctgggtgtc gaagtgacac
     1681 tcatcgacag ggcccagcgc cccctgtcgt tcatggacgc ggaaatcgtc gaggtgtttc
     1741 agcgcagcat cgaacggcaa ggcggccggt tttacgtggg acagaccgtc aaggaagtgg
     1801 tgtgggacgg cgtgtcctcc gtcgtcgcca ggctggcgaa cggcatggcg gtcaagagcg
     1861 aaaagatgct ggtcgcgctg gggcgtcaac cgaacatcga agaattgaat cttgaggcgg
     1921 ccgggcttac cctggacgag aagggccgga tccccgtcaa cgaatacggg caaacccccg
     1981 tcccgcatat ttttgccgcc ggcgacatgt tgggtcgtcc cccggccttg gcctcacaag
     2041 cgatggagga tggtcgtcgg gcggtcagtc acgccttggg ccttccagtc ggcgactcgc
     2101 tcaatcaggt gccgatcggg atctatacca ttccggaaat cgcctccatc ggactcgatg
     2161 aggaacaggc tgcagcccgg taccgtggcc cgctcgtggg gcgcgcgcgg tttacggaaa
     2221 tcgccaaggg gcagatcacg ggttcctgcg acggactcct gaagctgatt gccgacccct
     2281 ccggtgaacg gttgctcggc gtgcagatcg tcggcgagca tgcgaccgaa ttgattcacc
     2341 tcggacagat ggccttgcag gacggggcga ccatcgaccg gttcatcgat tccattttca
     2401 gctttcccac gtttgccgaa gggtatcgcg tggccgcgct cgatattctg ggccaacgcc
     2461 gcaagcgtca gagcaccact caagcggcct aatctcccgc cccctgccgt cttctccctc
     2521 tttgaccccc atgccctatg tgcgctactc tccggggcga cccgtaaggt cgccgcagga
     2581 gggatcgtcg tgaatcgcaa tgagcgccgc cgcgatgaaa aattgcatgg gaagcaggcc
     2641 ggaggtgccg atgccccggc ggcgctcctg cgcgaggcgc agctgcatca tcaggccgga
     2701 cgtctggatg aagcggaacg gggctaccgg tctctgctcg agcgcgcacc gacacagccg
     2761 gacgcacttc atggcttagg cctcctgacc taccggcgcg gcaatctgaa agacgcgctc
     2821 ggttggctgg cgaaagcttg tgccgcaggt ccgcgtaatc cgatctactg gttcaatcac
     2881 ggcgtcgtgc tgcaacgcgc aggacgcacg acggacgcgg tcgaggccta tggccaggcc
     2941 atccactgga atccccgcta catcgaagcc cgcaccaatc tcgggaacgc ctacaaagaa
     3001 ctgggacgtc tttcagacgc gcaagccgcc tatgagcagg tcctgacgct caaccccgat
     3061 catgccgagg cgcacaacaa tctcggcgtc gtgctgaaag aacaggggcg gttggatgaa
     3121 gccgcggagt cgtatcggcg tgccatcgcc ttgaagccga ctcatgcgga ggcacagaac
     3181 aatctcggcc tggtgctcct ggaacagggt cggttggacg atgccattcg ctgcttcgaa
     3241 cgcgcgcttc agatcgttcc cggttatgga accgcgctct acaacctcgg tattgcctgg
     3301 atctggcggg aggatatacc gcgggcgctg cgctgttttg ccgaaaccgc gcaagccaaa
     3361 catgcgcacg gccgccctgt ggcagagacg accgtctttc gctcccggct caagcatgat
     3421 gccgagcagc tagagtatct tcgagagagc gggctgttgg gcgatgagtg gaacccctat
     3481 cacgaggccc tcactcgact gtgcgaaaaa ttggaacact cggcgaccgg tgcgaccgga
     3541 tcctcaaatc gcgtgccgct gtcgcctgcc gacatgcagc ccattgcggc ctcgtataac
     3601 cggctgatcc atatcgctcc ttgcgaggcg atcgagggag gggcgctggc tgcggatctg
     3661 gacagcgcag ccgtggaggc ccgctatctg gcgacgaatc cggaggtgac gtacatcgac
     3721 gggttgttgg cggacgaagc gttgcaacgg ttgagacggt tctgctgggc gtccacgatc
     3781 tggaagaagg actacgagaa cggctatatc ggggccttcc tcggtgatgg attcgcctct
     3841 cccctgctgc ttcaaattgc cgaggagtta cgccgccggt ttccccgcat tttcgggacg
     3901 catcgcctga cgcaggcctg ggcattcaag cacgacagcg ccaggcgcgg cttgaacatt
     3961 cacgccgatg ccgcggcggt caacgtcaat ttctggatca cgccggatga ggcgaacctg
     4021 aatcctgaca gtggtggatt ggtggtgtgg gataaggaag cgcccaggga ttgggatttc
     4081 aagacgtaca atagcgacaa ggcccgaggt aagatttacg agtggctgaa cgcgcagggg
     4141 gcgaaggaaa tcaagattcc gtatcgggcg aaccgggcgg ttgtgttcaa ctcagatctg
     4201 ttccacgaaa ccgatgacat tgccttcaag gaagggttca ccaaccggcg tatcaatatc
     4261 acgctgctgt acgggcatcg gcatcgcccc tgagtaactt ctcaaaatct tccgcgcaga
     4321 caggccggct gaacaggtag ccttgcacct cgtcgcatcc ttcttcccgt aaccgtgcca
     4381 actggccttc tgtttccact ccttcggcca agaccgagag attcagactg tgcgccatcg
     4441 cgatgatcgc ccgggtgatc ttcacgttgt tttcgttcgt gagcagatcg cggacaaacg
     4501 attgatcgat cttcaatcgg ctcaggggaa aacgctgaag ataactgagc gacgaataac
     4561 cggtgccgaa gtcgtcgatg gagagacgga cgcccatggt gcggaggcct tccagcatgg
     4621 tgaccgacgc ctcgacgtcc cgcatggcga tggactcggt caattccagc tccagttgcg
     4681 atggattcag cccggagtcc cgaagcgcat cggctaccgt ggcgggcagc gtccgtccat
     4741 gaaacaggga attggacacg ttggccgaca cggtgatggg cggcaggccg gccgtttccc
     4801 aggctttcac ctggcggcag gcttcgcgaa gcacccattc gtccatcggg cggatgaggc
     4861 ccgtgtcgat ggccgcattc aagaagactc ccggcggcac caggccgcgt ttgggatgtt
     4921 tccaacggac gagcgcttcc gcgcccagta tcttgcgtga atggatgttc agcttcggct
     4981 gatagaagac gacaaactct tcccgttcca aggcgcggcg gagttcgttc tccaggtcga
     5041 ggcgttcggc cgcggcggcg ttcaagccgg acgaataaaa ctggcagttg ttccgcccct
     5101 gctctttcgc gtggtacatc gcggtgtcgg cgttcttgag cagcgcttcc acggtggcgc
     5161 cgtccgaggg aaagatcgaa atgccgatgc tcgcggaaat aaaaatttca tgcccgtcga
     5221 tgctgagggg gtgcgccaat gcctccagta tccgacgggc cacccggcct gcgtcttccg
     5281 gcgtcggcaa ggccgtgagc aaaatggtga actcatcgcc gccgagtcgc gcgagggcat
     5341 gcgtcggttc gtggtcggca tgccggccga ccgagtcgct ctgacgaacc gactcactca
     5401 accgttcggc cacatgtgtc agcaagcggt ctcccaccgt gtgccccagt gtatcgttga
     5461 tcaccttgaa ccgatccaag tcgataaaca gggtggccag atgctgatgg tagcgttcgg
     5521 cgtaggacag ggcgttggag aggcgatctt tgaacaacac acggttggcc aggcccgtca
     5581 ggctgtcgta gtatgcaagt tgatggatgg cccgttcagc ctgtttgcgt tcggtgatgt
     5641 cctgggcggt cccgatgacc gtcagctcgt ccgtctcttc ttcgcgcacc ccttcggcct
     5701 ggagatgaat gatgaagtcg gtcccgttcg gcaacacgac gcggtggtcg atatcgcatg
     5761 gcgtgtgctg cgagaccaat cgctccagcg cgccggtcac gaccggtcgg tcgtcggggt
     5821 gaatcagatt caggaaggcc tcgaaggtgc cgccgaaatc ctgcggacgc acgcccagca
     5881 ggcggcataa ttcattggac atgctcaaac gattggtctt gggattccag tcccagttgc
     5941 cgatccgggc gatccgctgg gccaattcca gccgcgattc gcttcggatg agcgcgtgca
     6001 gcacgttgtt ggttctgagc atgtaccgga catggtggca gagaatcgtg gcgttgaccg
     6061 gtttggtgat gaaatccgta gcgccgtgct gataggcctg cgcgatggaa ttcgcgtcat
     6121 ccaatcccgt gaggatcaaa atcgggacgc gtttgccgcg cggaagcgta cgaagccggg
     6181 aacaggtcag atacccgtcc atgtccggca tcataatatc caggacgacc agatcaggcg
     6241 ccagtgcctc gaatttcacc agcgcgtcct gccctccggt ggcctcggcc acggtcatgc
     6301 cggccggttc caatgcgttc cgaacaaaga gccgtgagag cgggtcgtcg tcggcgatca
     6361 ggatcgtggg acgggtttga gtcatcgtat ggtcttcagg cacaggtggt tatagcgcgg
     6421 gcgcatgggt tcccggggcg agaatgcctc gggtgcctct gacaggcctg cctgtttcgc
     6481 tctcgtgtga gagtatcaaa cttctgaata agcgctacat aattagcact tgttgagcag
     6541 cgcaggccgc tcccatccct aagtggaaat ctggtggatt caaagccgac ggcgggaatg
     6601 tccgtgccgg tgaatcaacc cctctccggt gtctcgacgg gattcccggc cgggacgaag
     6661 cgctgttccc cggcttccat gaggatgccg gccaagagtg cgcgaacccg gctcatgagt
     6721 cctcgcatgg tcagggtcaa gaggatgacc atcacgagga gcagcggtcc gatgaccagg
     6781 gccacgtccc gccccagatc atcctccgaa cgaagggctg cggcctccgg cggggttgcg
     6841 acaaccagtc tgatccatcc tgcgatacgg ttgtcctggc ggaagggctc gatcacgatc
     6901 atgaccggcc tccccttgtc taccccgtga gtgatggaac tgctctgact gttgcgtgca
     6961 gccggccatg cgccatcctg catcgggctg ccgatgatgg ccggattact ggcggccacg
     7021 atcatgttgt ccgttgtgat gacggcggca tcgagcagat tggcctgtct ggcatgttgt
     7081 tccagggtct gttgaacggt gcccagactc tcgccgggga gcgactcact gaaccaggcc
     7141 acggttcgcg ccatggcgcg tgcgctctgc tcggccgcca tgatggccgc cgcattgcgc
     7201 tgtgagacga gcgaacgatc gtgctgagcc aggagcacat agagcgccgc cgcgcagcag
     7261 gcggagacga tcacgctgat gaacaccgcc ggaagccagg tgcgaggcaa tcgggacgcg
     7321 tgagccatgc tgtatcctcc tcggtcaggg tcgtgacgtg ccgggaccgt cttgccggtc
     7381 gttggcccgt tcatagggtt ggcgcgtgag ttctttgaca atgccttgga ggcgagtcca
     7441 ttcgtccggt tgcagggaca cgaattcgat gccgatgcgg ccgtcgcggc accagcgtac
     7501 cacggcttgc tgaatggtca gagcggcggc atcaccggcg ggctggatgc gaagttcaag
     7561 agtggtgcct ggatgcacgg ctgtcgcgct caggatcccg caaccgcgca gggacagatc
     7621 gacgacattg ccctccccgc cgacgcggtt gaccgaactg aaggaactgc gaaagtgaac
     7681 tgaaaaacga ggatggtggc gttggtccat gatcggctag cgctggtaca aggctagccc
     7741 tttatattgc cgattggttt gagttccgct actttttttg tgattcccgc gtgatccacc
     7801 gtctcgacga catcggaaat gttcttgtac gcgaacccgg cttcttcagc cagacccgac
     7861 atggatacgg ctttgaccag aatgccgtgc tgtttcatgt cgcgcatgag ctgctcgccg
     7921 cgcaccgtgc gtttcgcctg cgcgcgcgac atggtccggc cggacccgtg catggtcgag
     7981 ccgaacgtct cctgcatcgc ccgatccgtg ccgacgagaa gatacgagcc ggtctccatg
     8041 gagccgccgc agatgaccgg ctggccgatc cctcgataac aggccgggag ttcagggctc
     8101 ccgggtccga atgcccgcgt ggcgcctttc cggtgcacga gccactcccc gtcccggtag
     8161 cgttccacct tggcgatgtt atgcgccaca tcgtagatca gctgcatgcc caggcgccgg
     8221 gcgctttctc cgaacaccgc ttcgaaggcc tcgcgaatct gatgcgtgat gacttgccgg
     8281 ttggcgaacg cggtgttggc cgcgcagttc atcgcgccga aatagtcctg tccttccggt
     8341 gagcgaaacg gcgcacaggc gagttgctga tctttgacgg agatcccgta ccggcgcatg
     8401 gctttctcga acaccttgag gtagtcactc gccacttggt ggccgaatcc gcgcgaaccg
     8461 caatggacca tcacgacaac ctgatcgcga ccggtcaacc ccatggccgc ggcggtcgca
     8521 cgatcgtaga tccctttgtc ggagagcacc tgtatttcca ggtagtggtt gccggatccc
     8581 agggtgccga gttggttgat gccgcgttcg atggcgtagt ccgtcacatg ggtggggtct
     8641 gcgccctcca gacaaccgcg ttcctcgatg cgttcgaggt cctcctccca gccatatcct
     8701 ttggagatgc accagccggc tccctgccgc ataacgtcgc gaaagtcatg ccgggaaaga
     8761 ttcacgaagc cgcgcgatcc gacaccggcc gggacgcgcc ggaagagttc cgtcatcagc
     8821 agttccagtt tcggttgcac ctcggagagc gacaagtcgg tacggatcag ccgcatcccg
     8881 cagttgatat cgtagccgac gccgccgggt gagatgactc catcctcagg attgaatgcg
     8941 gccacaccgc cgatgggaaa gccgtacccc caatgtccat cgggcataca gagggcataa
     9001 cgatggatac cggggagaca ggccacgttg gtgacctgat cgaagacgcc cctatccatc
     9061 gcaccgagga tcgccgcgct ggcgtagatg cgagccggga ccagcatgcc cggcttttca
     9121 gagggcggaa tttcccagag gtagtcgttg atcttgacga cctgcatatc ggtgttcagt
     9181 ttcatacgtc cagccggatg ctgaaaaagg cctccaacgt cgtgctcggg cacttaatcc
     9241 cctcgacgta cgctgagtac gcctcggggc cttcgcgtcc tgcggcctag ttggcgatcc
     9301 tttttgagca tccttgtatt gaatgaaggt gattctgcgc gaaggggtgt ttcatacgtc
     9361 cagcaccacc ctggccttcc agctcgtccc ttcttgtgtg accgcataca gatgtttggt
     9421 gacgcctttg acgtctgagc gcaggtcctg cgtcgcggga tcgaccggtg caccggtcag
     9481 ttcggcatgg aggtgccagg ctgtgtgggc atcattctga ttcagcgaca atgtcgcccg
     9541 atggaagacc acgccctgcg catctttcaa atagacgagc cgtcccagcc attcgaacag
     9601 gagcgtctcg atgtccgctt cttccatgtc caggctgtgc ctccacgttt cggcgatggt
     9661 gacaggatcg gccaggcttt cgatgagagc ttgcgtcgcg gcctcgaaga gggccgacaa
     9721 ggactctccc tcggcatcga atgccatatc ggccagcgcg atgtcgtcga ggaatcggaa
     9781 ggagcccggc atgggcgtca gacgcgagga gcccgacggc gcgcggcggc aaagatggcc
     9841 cggacctgtt cgatgccagg aatggtgtgg ccgaacggca acggtacctt tcctttgagc
     9901 cacatacgca cgccgagcgg catgacatcg agcacgcgtc gaagattgaa cccaaggact
     9961 ttcagcggca tcaacgcttc attcaaacgg ccctcgtggc ggaccaggtc gacgaagccg
    10021 gtgatgtgac gagcgccttc ggctgaggtc aatccatgcc ggagggacga gcgacgcaat
    10081 cgaatgatcg cctccatcgg ctggacatcc ttcgggcaga cttgcacgca catgttgcag
    10141 cgggtgcagt cccagatgcc attagactcc tggagcgcgg tgagtctggc ctgtttcgcc
    10201 ccggccggtt cgcgcgggtc tgcgacgaat cgcgcggact tcgcgagcgc cgccgggccg
    10261 agaaaacccc gtgagacttc atgcgacgtg caggcggcca cacaggctcc gcacatgata
    10321 caggcgtcta cgttatgaaa ctggtacgac tccggcagca gccgaagttg tccggatgac
    10381 ccgtatcgtt tggtgggatg ggtctcgggc gtcagccagg gcgtgacggc ccggatcttc
    10441 tcccagaacg gcgacatatc gaccaccagg tctttgatga ccgggaggtt gggcaagggc
    10501 tcgatcgaga ttctgccgtg gcgttcgagt tctttgcgaa tggaggtccg gcaggcgagc
    10561 ttttccgtgc cgttgatctg catggcgcag gagccgcaaa tggccgagcg acaggaataa
    10621 cgcagcgcca accggccgtc gagttcgttc ttgatgcgga tcagggcctc gagcacggtc
    10681 atgccgcgac cgatgtcgag gcgatagtct tcccggtggg gctgttgatc ggtttcggga
    10741 ttaaaacgct ggatggtaaa ggtcaggcgc ataacatcgt gaggcgtgag acgtaaaacg
    10801 tgaaacggga aaagagagcc gcccctatgt tgagggaccg cttaccatgg gtcagccgag
    10861 ttcgaagact ttcgggtaat tcgtcaaatt ccggcag
//
LOCUS       DAOQRM010000082        10638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_5484_length_10638_cov_13.106775, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000082 DAOQRM010000000
VERSION     DAOQRM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5484_length_10638_cov_13.106775"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..79)
                     /locus_tag="PKK81_08010"
     tRNA            complement(3..79)
                     /locus_tag="PKK81_08010"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43..45),aa:Pro,seq:tgg)
     gene            complement(70..684)
                     /locus_tag="PKK81_08015"
     CDS             complement(70..684)
                     /locus_tag="PKK81_08015"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HNV32859.1"
                     /translation="MQIVLATRNQHKKQELVALLGGMDITIRTLDDFPDAPDVVEDGE
                     TCEANAMKKAVEIARYTGLPAVADDTGLEVDALGGRPGAFAARYAGEQASYEDNWRKL
                     LQELRGIPAEDRGARFITVAAIAFPAGKTFSTKGVLEGVIAEEATGSRGFGYDPVFFL
                     PEYHQTLAQLSPDVKNRISHRARAFTQARTLLEQMMAEQNSVGA"
     gene            complement(687..1430)
                     /gene="rph"
                     /locus_tag="PKK81_08020"
     CDS             complement(687..1430)
                     /gene="rph"
                     /locus_tag="PKK81_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNV32860.1"
                     /translation="MASGAGLGRIDGRRRDQIRPVRVTRNFTKHAEGSVLIEMGDTKV
                     ICTASIEEKVPPFLKGKGAGWVTAEYAMLPRATHDRSPRESVKGKQGGRTLEIQRLVG
                     RALRAVIDTGRLGERTIWIDCDVIQADGGTRTASITGSFIALADAVGVLKKKDLIKVN
                     PLTDYLAAISIGKVGGQVMVDLAYEEDSHAEVDLNLVMTGAGQYVEVQGTAEKTPFNK
                     KDMDEFLDLGWGAIRELVDLQKSLIGSLS"
     gene            1902..3080
                     /locus_tag="PKK81_08025"
     CDS             1902..3080
                     /locus_tag="PKK81_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV32861.1"
                     /translation="MSIPSVTEVTTQQAILENRNILIVDDEEPIRRLLGYLLEPHGYK
                     VALAGEAREARQRMESGSYALVLCDVNMPGESGMDLIRHILTQYPSTAVIMITGLDSP
                     VLANAALDMGAFGYVIKPFEANEVLINVANALRRRKLEIENAMHRENLEEVVRTRTIA
                     LQQALEWLERSEKELRLSREETIQRLAIAAEYRDSSTAQHIQRMSHYCELLARRYGLS
                     PERCDLIRTASPMHDIGKIGTPDHVLLKPGKFTPEEFKVITQHTEIGYRILAGSDSEL
                     LKVAALIAWTHHERFDGTGYPRGLKGETIPLEGRITAIADNFDALTTQRVYKPAYDFD
                     HAKELMLKERGKHFDPELLDIFFDSMEEITRIYDQFADPTWLSTSRHRAITQDQGEVA
                     "
     gene            3077..>3330
                     /locus_tag="PKK81_08030"
     CDS             3077..>3330
                     /locus_tag="PKK81_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32862.1"
                     /translation="MTRTSRNWIAYLCESLASSGMMPTWEAATYLTDNLFGDKPNPRL
                     LRTACPYEDTTHLLHRLYQPLVEAATRDIPIPAAAMIHVFT"
     assembly_gap    3331..3430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3431..7108)
                     /gene="metH"
                     /locus_tag="PKK81_08035"
     CDS             complement(<3431..7108)
                     /gene="metH"
                     /locus_tag="PKK81_08035"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNV32863.1"
                     /translation="MPVHEVRDLLRERILILDGAMGTMIQRYKLDEAAFRGERFRNWT
                     KDLKGHNDLLNVTRPDVIEAIHRQYLEAGADIIETNTFNSQSVSLADYGMDDLGYELS
                     KAGAECAKRAVAQVVAAQPTRRCFVAGAIGPTTKTSSVSTDSNDAAARGTTYPELVQA
                     YGEQVRGLLDGGVDLLLVETIFDTLNAKAAFFAIQQLFAEGARQVPIMASVTFIQAGS
                     NRGFSGQTVEGFWNSISHVPLLSVGMNCALGPKEMRPLIEELSQIAPIFVSSHPNAGL
                     PNPLLPTGFPETPDSLAPQLREWAQNGWLNIVGGCCGTTPDHIRSIATAVKGVTPRQP
                     SKVEPYLRLSGLEALTVRPESNFVNVGERTNITGSPAFSKLILAGDYDKALTVARQQV
                     EGGAQVIDINMDEGMLDSKAAMQKFLRLLAAESDIARVPIMVDSSKWEVIEEGLRNIQ
                     GKGIVNSISLKEGEAKFLEQARLIRRYGAAIVVMAFDERGQADSVARRIEICERSYRL
                     LTEQVGVPPQDIIFDPNILTVATGLEEHNNYAVDFIDATRWIKQHLPLAKVSGGVSNI
                     SFSFRGNNVVREAMHAAFLYHAIQAGLDMGIVNAGQLAVYEEIPKDLLTLVEDVLLNR
                     RPDATERLVAFADTVKQKGKTAVKDDEWRTRPVEERLAHALVKGLTDYIDQDVEEARQ
                     KSSRPLDVIEGPLMAGMNIVGDLFGSGKMFLPQVVKSARVMKKAVAYLMPFMEAEKLR
                     LGTSRANGKVLLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVSCEKILAAAREHQ
                     VDMVGLSGLITPSLDEMVHVAKEMTRQGFTVPLLIGGATTSKAHTAVKIAPSYGHATV
                     HVLDASRAVGVVGSLINEEQRESFSAGVRADYDRIRQAHQDRGPKTLRSLEAARKHKL
                     STDWAAADIPTPSFTGVRQIDRMPLRELVPYIDWSPFFHTWELRGRYPAILDDATVGP
                     KAKELLADAQALLEEIIQGELLTARGVYGFFPANSAGDDIHLYRDPDRREPLATFHTL
                     RQQMEKPTDQFNLALADYVAPKESGRADYVGAFVVTAGIGVPALCAKYEKDHDDYNSI
                     MVKALADRLAEAFAEWLHKQVRLEWGYGKTEDLTNEDMIRERYRGIRPAPGYPACPDH
                     TEKRTLFDVLNAEAQAGVTLTESYAMQPAAAVSGLYFAHPEAKYFAVGKINRDQVEDY
                     ATRKNLPVGEVERWLAPNLNYD"
     gene            7296..8075
                     /locus_tag="PKK81_08040"
     CDS             7296..8075
                     /locus_tag="PKK81_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32864.1"
                     /translation="MAHQTVSRLFILCLFCITIVLSRLDPLWAGPYFEDGYLGLTQTE
                     LHDKLGLPQAVRDRKSALRVFTYYPITDWEQYFKKVASPENGEDVYTFKRDGIDVRYS
                     FSYTFDPNDHNDQRPLFVRLVDIEFMPPVALSKVPQLVPEFKPSEDANAPVFRSNIML
                     LFFAGNPSPEARFLVREKGKEALDWSLAFQMFALQGLPDPLTSKAPIDRLEISTQSLP
                     LVKLRQRNTHEPVLNPYSKAFAQQPPPPPTVKKIPVPKYAD"
     gene            complement(8173..8826)
                     /locus_tag="PKK81_08045"
     CDS             complement(8173..8826)
                     /locus_tag="PKK81_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32865.1"
                     /translation="MGCWRYSINRCWIGLIMLGICLTWYGVAGAGPLYVYTDAQGQAV
                     LTDNLEQVPAEFRGRVRTMATAEAPPTAAPAPVAAATVSQSPSASGVVEHLLNVIAAK
                     VGSRTIKGLTAHQTAVIILAGVCWLALLVPIFMSSNPGIRLLCKFLLVLVGVAALYQV
                     AISGMPSFEAVTGSPQQGSEQAMENVLGKMRSQTEQSYLAQDARTARQLEQIEPPTQ"
     gene            8996..9448
                     /locus_tag="PKK81_08050"
     CDS             8996..9448
                     /locus_tag="PKK81_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32866.1"
                     /translation="MTDQTTSATPVSALPPAVEPTPFIRQRPVRIIIHTFLGLFVVAL
                     IAFRFVPGWLDPDFSKHIESHRVMVGMDRKQVLDSWGSPNTMNVTHTSDGLRREEWIF
                     EDWESPAVVKHRYLYFEEGKLIGGHFSGSDVRLPIPTTPEPVKPKGHP"
     gene            complement(9487..9756)
                     /locus_tag="PKK81_08055"
     CDS             complement(9487..9756)
                     /locus_tag="PKK81_08055"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNV32867.1"
                     /translation="MPRHHPENSTKLVTLCESGDVGELALIKSLLDGNGITYTVQHEH
                     VGALYPGVALLGSRVMVEERDKQRAEVLLSRLALHIRETTGDVDG"
     gene            complement(9986..10270)
                     /locus_tag="PKK81_08060"
     CDS             complement(9986..10270)
                     /locus_tag="PKK81_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32868.1"
                     /translation="MKPITIQDSDQILNFLAEVALHGKGFTTDCLLDYVLDEGFTEPI
                     YLNASGEDPDAFYKDQPQAWAIYQVREWKRVLTVSGGAGKERRVQITETP"
     gene            10318..>10638
                     /locus_tag="PKK81_08065"
     CDS             10318..>10638
                     /locus_tag="PKK81_08065"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNV32869.1"
                     /translation="MAHKRLTRTQFLERLLAIMDCKHHWAWPIIMGPGITKAQLKLHY
                     QQEFLVYVRDFPVLLARVHGQNPPSDVRRMLAENIYEEDTGGLSFGRSHPELFNEMMR
                     GLGFD"
BASE COUNT         2167 a         3419 c         2845 g         2107 t
ORIGIN      
        1 tttggtcggg gcgagaggat ttgaacctcc gacatcctgg tcccaaacca ggcgcgctac
       61 cgagctgcgc tacgccccga ctgagttctg ttccgccatc atctgctcga gcagagtcct
      121 ggcctgggta aaggcacggg cccgatggct gattcgattt ttcacatccg gcgacagctg
      181 ggccagagtc tgatgatatt ccggcaggaa aaagaccgga tcatacccaa aaccacggga
      241 gcccgtcgcc tcttcggcaa tgactccctc taacacccct ttggtcgaga aggtcttgcc
      301 ggcaggaaac gcaatggccg ccacggttat aaagcgggca ccacgatctt ccgctggaat
      361 accacgaagc tcctgcaata gcttccgcca attgtcttca tagctggcct gttcaccggc
      421 gtaccgcgcc gcaaaggctc ccgggcgccc gccgagggca tcgacctcca atccggtatc
      481 atctgccacc gccggcagtc ccgtataccg agcgatctcg acggcctttt tcatcgcgtt
      541 ggcttcacag gtctcgccgt cctccaccac gtccggcgcg tcgggaaaat cgtccagtgt
      601 acgaatcgtg atatccatac cgcctaatag ggcgaccagc tcctgcttct tatgctgatt
      661 cctggtcgcc agcacgatct gcatggttaa ctcagcgagc cgatcaacga cttttgcaaa
      721 tcaaccaatt cacggatcgc gccccagccg agatcgagaa actcatccat atctttctta
      781 ttgaacggcg tcttttcagc cgtcccctgc acttcgacgt actgcccggc gccggtcatc
      841 accagattca aatccacctc ggcatgcgag tcttcttcgt aggctaggtc caccatgacc
      901 tgtcccccga ccttgccgat actgatggcc gccaggtaat cggtcaacgg attaaccttg
      961 atcaagtcct ttttcttcag cacacccacg gcatccgcga gagcgataaa ggagccggta
     1021 atggaggccg tgcgagttcc accatccgct tgaatcacat cacaatcgat ccagatggtc
     1081 cgttcgccca aacggcccgt atcgatcacg gcacggagcg cacgtccgac caaccgctga
     1141 atctccaacg tgcggccgcc ctgcttaccc ttgaccgatt cccggggcga acgatcgtgc
     1201 gtggcccggg gcagcatggc atattcggct gtcacccagc cggcaccctt accttttaaa
     1261 aacggcggaa ctttttcttc aatcgacgcc gtgcaaatga ccttagtgtc ccccatttcg
     1321 atcaagacgg agccttcggc atgtttcgta aaattgcgcg tcaccctaac cggacggatc
     1381 tgatcccgcc gacggccatc gattcgacct aaccctgctc cgctcgccat tgagtcgcgc
     1441 tccttcctgc acagcaaatc taaaggagcg aattatagtg aacagggctt caaagagcaa
     1501 atccagcggc gcgaccggtg ctcgtttctc aaactgcgtt tcggctggca atcgggaaag
     1561 ccccctgtat aatccagcgt gatggtgcag gttgctgttc cctcgggaac gccgcctgct
     1621 caaagaccaa cggacgagcg tcagagttga cgatcacagc cccgggccgg gcagagcggc
     1681 cggaaactgc cactcgtcgg atcttgtccc gatagaacag agttcccagc attgatgcag
     1741 caatgggcgg gcggcacggg acagttggtc gcaccgcggc ttgagtggca taaaaggtgc
     1801 ttttcccctc aatccatctc gcacttcaga ctttacgccc aacgaccgat actgactcta
     1861 ttgctgatcg acaccacgat taacctagcc cggcgaacca tatgagcatt ccgagcgtca
     1921 ccgaagtcac cacccagcaa gcaatcctcg aaaaccgcaa catcctgatt gtggacgatg
     1981 aagagcctat ccgtcgatta ttgggctacc ttctcgaacc ccacggatac aaggtggcgc
     2041 tggcgggaga agcgcgcgaa gcccgtcaac ggatggagag cggttcctat gcgttggtgc
     2101 tgtgtgacgt gaacatgccg ggcgaatccg gcatggatct cattcgccac atcctgacgc
     2161 aataccccag caccgccgtc atcatgatta cgggcctcga cagccccgtc ctcgcgaatg
     2221 cggccttgga tatgggcgcc ttcggttacg tcatcaagcc gttcgaagcg aacgaagtcc
     2281 tgatcaacgt ggccaacgcg ttgcgacgac gcaagttgga aattgaaaac gccatgcacc
     2341 gggagaatct ggaagaagtc gtccggacca ggacgattgc ccttcagcag gcacttgagt
     2401 ggctggagcg gagcgagaag gaactgcgcc tctcccgcga agaaaccatt caacggctcg
     2461 cgatcgccgc cgaataccgc gacagttcga ccgcgcagca tattcaacga atgagccact
     2521 actgcgaact gctggcccgt cgctacgggc tttcacccga gcgatgcgat ctcatccgca
     2581 ccgccagtcc gatgcacgac atcggcaaga tcggcacccc ggaccatgtg ctgctcaagc
     2641 caggcaagtt cacacccgaa gaattcaagg tgatcaccca gcatacggaa atcgggtacc
     2701 ggattctggc cgggtctgat tcggaactct tgaaagtcgc cgcgctcatc gcctggacgc
     2761 accacgaacg cttcgacggc accggttacc cgcgcggact caaaggcgag acgattccgc
     2821 ttgaaggccg gatcaccgcc atcgccgata acttcgacgc cctgacgacg cagcgtgtct
     2881 acaagccggc ctatgatttc gatcacgcga aggaactgat gcttaaggag cggggcaagc
     2941 acttcgatcc ggaactgctg gatatcttct tcgactcgat ggaggagatc acacgcattt
     3001 acgatcaatt tgccgatccg acctggttgt ccacctcccg tcaccgcgcc atcacgcagg
     3061 accagggaga agtcgcatga cccgcacatc acgcaattgg atcgcgtatc tgtgcgagag
     3121 cctcgcctcc tccggcatga tgccgacctg ggaagccgcc acctacctga ccgacaacct
     3181 gttcggcgac aagcccaatc cccgactctt gcgaaccgcc tgcccctatg aggatacgac
     3241 gcacctgctg caccggttgt atcaaccgct ggtcgaggcc gccacgcgcg atatcccgat
     3301 tcccgcagca gccatgatcc acgtcttcac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn atcgtagttc agatttggag ccagccaccg ctctacttcg ccgaccggta
     3481 gattcttgcg ggtcgcgtaa tcctccacct gatcgcgatt gatcttgccc acggcaaaat
     3541 acttggcctc cggatgcgca aaatacaagc cgctgaccgc agccgccggc tgcatggcgt
     3601 aggattcggt cagcgtcacc cccgcttgag cctcggcatt caatacatcg aagagcgtcc
     3661 gcttctccgt gtgatccgga caggccggat aaccgggagc cggccggatc ccccgatacc
     3721 gctcgcgaat catgtcttcg ttcgtgaggt cctccgtctt cccatatccc cattccagcc
     3781 gcacctgctt gtgcagccat tcggcaaagg cctccgccag ccgatccgcc aaggccttga
     3841 ccatgatgga attgtagtcg tcatgatcct tctcatactt cgcgcagagc gccggcacgc
     3901 cgatgccggc cgtcaccaca aacgcgccca catagtcggc acgcccggac tccttgggcg
     3961 cgacataatc cgcaagcgcc aggttgaatt ggtcggtcgg cttctccatc tgttgccgga
     4021 gcgtgtggaa agtggccagc ggctcacgtc gatccggatc ccgatagaga tgaatgtcgt
     4081 cacccgcgct attggcggga aagaacccgt agacaccgcg agccgtcagc aactcaccct
     4141 ggatgatctc ctccagtaac gcctgtgcat ccgccaagag ttctttggcc ttcggaccca
     4201 ccgtggcatc gtccaggata gccggatacc ggccgcgcaa ttcccacgta tgaaaaaacg
     4261 gcgaccaatc gatatagggc accaactcac gcaacggcat ccgatcgatc tgacgcacgc
     4321 cggtgaatga aggcgtgggg atatcggcag cggcccaatc agtcgacagc ttgtgcttcc
     4381 gggctgcttc caggctacgc agggttttcg gccctcgatc ctgatgcgcc tgccggatgc
     4441 ggtcatagtc ggcacgtact ccggcagaaa agctctcccg ttgctcttca ttgatcagac
     4501 tgcccacgac accgacggcg cgggaggcgt cgagcacatg caccgtcgca tggccgtacg
     4561 aaggggcaat tttcacggcc gtgtgggcct tgctggtcgt ggcgccgccg atgagcaatg
     4621 gcaccgtaaa cccctggcgc gtcatctctt tggccacatg caccatttca tccaacgatg
     4681 gagtgatcag gccgctcagc ccgaccatat ccacctgatg ctcacgcgcg gccgccagaa
     4741 tcttttcgca agacaccatc acgccaaggt cgatcacctc atagttgttg cagcccaaca
     4801 ccacacctac gatgttcttg ccgatatcgt gcacatcgcc cttgacggtc gcgagcagga
     4861 ccttgccgtt cgcgcgggac gtgcccaacc gtaacttttc ggcttccata aacggcatga
     4921 gatacgccac cgctttcttc atgactcggg cgctcttcac cacttgcggc aggaacattt
     4981 ttcccgaccc gaagagatcg ccgacaatgt tcatgcccgc catcagcggc ccctcgatca
     5041 cgtcgagcgg ccgggaggac ttctgcctcg cctcctccac gtcctgatcg atgtagtcgg
     5101 tcagtccttt caccagcgca tgggccaacc gttcctccac cggtcttgtc cgccactcat
     5161 catccttgac cgccgtcttg cccttctgtt tgaccgtgtc ggcaaaggcc accagccgct
     5221 ccgtggcatc cggccggcga ttcagcagca cgtcttcgac cagcgtcagc agatctttgg
     5281 ggatctcctc gtagacggcc agctgccccg cattgacgat gcccatgtcg aggccggcct
     5341 gtatggcatg gtagaggaac gcagcatgca tcgcctcgcg cacgacgttg ttgccgcgga
     5401 acgagaacga aatattgctg acgccgccgc tgaccttcgc cagcggcaag tgctgcttga
     5461 tccaccgtgt cgcatcgata aaatcgaccg cgtagttgtt gtgctcttcg agtccggtcg
     5521 cgaccgtgag aatattcgga tcgaaaataa tatcctgcgg cggcaccccg acctgttccg
     5581 tcagcagacg ataggaccgc tcacagatct cgatccgccg tgcaaccgag tcagcctgcc
     5641 cccgttcatc gaacgccatg accacgatcg ccgcgccgta ccgacggatg agccgcgcct
     5701 gttcgaggaa cttcgcctcg ccttccttca gactgatgga attgacgatg cccttcccct
     5761 gtatgttgcg aaggccctct tcgatgacct cccacttgga actgtccacc atgatcggca
     5821 cacgggcgat gtccgattcc gccgccagca gccgcagaaa cttctgcatg gccgctttgg
     5881 agtccagcat gccttcgtcc atattaatgt cgatgacctg cgccccgcct tccacctgct
     5941 gacgcgcgac ggtcagcgcc ttgtcataat cgccggccag aatcagcttg gaaaaggccg
     6001 gtgagccggt gatattggtg cgctccccga cattgacgaa attcgactca ggccgcaccg
     6061 tcaaggcctc caggccgctc agccgcaagt agggctcgac cttggagggc tgccgtggcg
     6121 tcacaccttt cacagccgtg gcgatcgaac ggatatggtc gggcgtcgtc ccgcagcagc
     6181 cgcccacgat attgagccag ccgttctgcg cccactcgcg cagctgtggt gccaacgaat
     6241 ccggcgtttc cggaaatcct gtcggcagta agggattggg caatccggca ttcggatggc
     6301 tgctgacaaa aatcggcgcg atctgcgaga gctcttcgat cagcggccgc atttctttcg
     6361 gccccaatgc gcagttcatg cccacgctga gcaacggcac atgcgagatg gaattccaaa
     6421 acccttcgac cgtctgaccc gaaaacccac ggttgctccc ggcctgaatg aaggtgaccg
     6481 aggccatgat cggcacctgc cgcgcccctt ccgcaaacag ttgctggatc gcgaaaaatg
     6541 cggccttggc gttgagcgta tcgaaaatcg tctcgaccag gagcagatcg acgccgccgt
     6601 ccaacaggcc gcgcacctgc tccccgtagg cctgcaccaa ttcaggatag gtcgttccac
     6661 gcgccgccgc atcgttcgaa tcggtcgaga ccgaagaggt cttggtggtg ggaccgatcg
     6721 caccggccac aaagcagcgg cgagtcggct gcgcggcaac gacctgtgcc acggcccgct
     6781 tcgcgcattc ggcgccggct ttcgacaatt catatcccag gtcatccatg ccgtaatcag
     6841 ccagcgacac agactgcgag ttaaacgtat tggtttcgat gatatccgcg ccagcttcaa
     6901 ggtactgccg gtgaatcgcc tcaatgacat ccggccgggt gacattcaga agatcgttgt
     6961 gtcccttgag atctttggtc caatttcgaa aacgctcccc acgaaacgcg gcttcgtcca
     7021 gcttataccg ctggatcatg gtacccatgg caccatccag gatcaggatg cgctctcgca
     7081 ggaggtcgcg aacttcatgt accggcatga ctacctatgt ctttctgcca ccggacgtca
     7141 ggcgtgcgcc gtctgcgtcg atgactggca attggacggc catcatatcg ggaacggact
     7201 tttttcagca agctattgca ggctcttcgc cttgacattg actaccccgg ccgatacgat
     7261 gcccaccgtt gtctgcccga gcctgagaca atcctatggc gcatcagact gtctcccgcc
     7321 tgttcatcct ctgcttgttc tgtatcacta tcgtcttgtc gagactcgat cctctgtggg
     7381 caggccccta cttcgaggac ggctacctcg ggctcacgca aacggaactg cacgacaagc
     7441 tcggtctgcc gcaggccgtc cgggaccgca aatctgcgct gcgtgtcttc acgtattatc
     7501 cgatcaccga ttgggagcag tacttcaaga aggtggcctc accagaaaac ggagaggatg
     7561 tgtacacctt caaacgtgac ggcatcgacg tgcgctattc gttcagctac accttcgatc
     7621 cgaacgacca caacgaccaa cgcccgctct ttgtgcgttt ggtggatatc gaattcatgc
     7681 cgccggtcgc gttgagcaag gttccccaac tcgtcccgga gttcaagccc tccgaagacg
     7741 ccaacgcgcc ggtcttccgt tcgaacatca tgctgttgtt tttcgccgga aacccttcgc
     7801 cggaggcgcg ctttcttgtg cgggaaaagg ggaaggaggc cttggactgg tcactcgcct
     7861 ttcaaatgtt cgccctgcag ggactacccg atccgctgac ctcgaaggcc ccgatcgacc
     7921 gcctggagat cagcacgcag agtttaccgc tggtcaagct ccgccaacgc aacacccacg
     7981 agccggtcct gaacccttac tccaaggcct tcgcccaaca gccgcctccc ccgccgaccg
     8041 taaaaaagat tccggtgccc aaatacgccg actgagaggt tccggacggt cctgctccct
     8101 gccctctcga agctacagag tgggcgaaag ccacacgcct tgctgcaagt ctgtctcatc
     8161 ccaccgggat cgttactggg taggcggttc gatctgctcc aattgacggg ccgtgcgggc
     8221 gtcttgcgcg agataacttt gctcggtctg gcttctcatt ttccccagca cattctccat
     8281 ggcctgttcc gatccctgct gaggcgagcc ggtcaccgcc tcgaaggaag gcatgcctga
     8341 gatcgccacc tgatagagag ccgcgacgcc gaccagcacc aggagaaact tgcagagcag
     8401 ccggatgccg gggttggaac tcataaatat cggcaccagc agtgccagcc agcagacgcc
     8461 ggccaggata atgacggcgg tttgatgggc cgtgaggccc ttgatcgtgc gggacccgac
     8521 ttttgccgcg atgacgttga ggaggtgctc caccaccccg gaagcagacg gtgactggct
     8581 gacggttgca gcagccacag gcgcgggagc agcggtcggc ggagcttccg cagtggccat
     8641 cgttcgcaca cgcccgcgaa actctgccgg tacctgctcg aggttatcgg tcagcacagc
     8701 ctgtccctgg gcatcggtgt agacatagag cggtcccgcc cccgccacgc cgtaccatgt
     8761 caggcaaatc cccagcatga tcagcccaat ccaacatcgg ttgatcgaat aacgccagca
     8821 ccccatgcgt cgccctccgc ggtcctgctt caagcatagc aggtcgaaca gccggctcaa
     8881 gggagtgata cccgtctctg atgccctccc cctcccttga cggagcgaaa aacgattgtt
     8941 tagcatgcag aagtaccagc actcttcttg cccattcgat gacggaagga ccattgtgac
     9001 cgatcagacg acctctgcaa ctcccgtttc ggccctcccc ccggcggtgg aaccgacacc
     9061 cttcatccgc cagcggccgg tccggatcat tatccatacg tttctggggt tattcgtggt
     9121 cgccctgatc gcgtttcgtt tcgtgccggg ctggctggat ccggacttct cgaagcacat
     9181 cgaaagccac cgagtgatgg tcggcatgga ccgtaaacag gtcctcgact cctggggctc
     9241 cccgaatacg atgaatgtga cgcacaccag cgacgggctg cggcgcgaag aatggatttt
     9301 cgaagactgg gaaagtccgg cggtcgtgaa gcaccgctac ttgtatttcg aagaaggcaa
     9361 gctgatcggc gggcattttt cgggatcgga tgtgcgcctg cccattccca ccacacctga
     9421 gccggtcaaa ccgaagggtc atccgtaggc ggccgttgac cggccggtag cccgctgctc
     9481 aagacgtcat ccgtccacat caccggtcgt ttcacgaatg tgcagggcca gacggctgag
     9541 cagcacctcg gcccgctgct tgtcccgctc ctccaccatc acccggctgc ccaacagcgc
     9601 gaccccgggg tacaacgccc cgacgtgctc atgctgcacc gtgtaggtaa tcccattgcc
     9661 gtcgagcagg ctcttgatca acgccaactc accgacatca cccgactcac acagggtgac
     9721 cagcttcgtg gaattttcag ggtggtgtct tggcatgaag gtcggcctcc cggtcggcag
     9781 ttcgaagcca cattccgatc aggcttgtct ggaaccggca ggccgggcgg ctgacagtgc
     9841 gcgattcgtt gcaattctgt cggttggtca atacactgac tgatgagtga gtactgcacc
     9901 ttccatcagc tcggccatac cacatgcgag cgaggcgaga agccatccac cggttggctg
     9961 gcaccggcgg tacgacacgg acgacttacg gggtttcagt gatctgcacg cgccgttcct
    10021 tcccggcgcc ccccgacaca gtcaacactc gcttccactc gcggacttgg tagatcgccc
    10081 aggcctgagg ctggtcctta tagaaggcat ccggatcctc accggaggcg ttcagataga
    10141 tcggttcggt aaatccttcg tccaggacat aatccagcag gcagtcggtg gtaaacccct
    10201 tgccgtgcag ggccacttcg gcgaggaagt tgagaatctg gtcggagtct tgaatcgtga
    10261 taggcttcat ctgatcggtg gctgattgta gccagtgaga aagaaggaag gcaaggcatg
    10321 gcacacaaga gattgacccg cacacaattt ctcgaacggc tgttggcgat catggactgc
    10381 aaacaccact gggcctggcc gatcatcatg gggcccggca tcacgaaagc ccagctaaaa
    10441 ctccactatc aacaggagtt cctggtctac gttcgcgact tccctgttct gctggcccgc
    10501 gtgcacgggc agaatccgcc ttccgatgtg cggcgcatgt tggcggagaa tatttatgag
    10561 gaagataccg gcgggctctc cttcggccga tcccatcccg aactctttaa cgaaatgatg
    10621 cgcggcctgg gcttcgac
//
LOCUS       DAOQRM010000083        10534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_5571_length_10534_cov_13.231033, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000083 DAOQRM010000000
VERSION     DAOQRM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5571_length_10534_cov_13.231033"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PKK81_08070"
     CDS             complement(<1..191)
                     /locus_tag="PKK81_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32870.1"
                     /translation="MTRKPSLAMIADCARPLANLVRGRPVLATFQVTLRCNSACGYCD
                     LPLNQGRYELTREEIRGIFG"
     gene            392..805
                     /locus_tag="PKK81_08075"
     CDS             392..805
                     /locus_tag="PKK81_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32871.1"
                     /translation="MNTKLQASILTIAAASLATVAFVVAPASAKDKEKAGKHSNHATT
                     TSAAAASSLLRSVPEQPPAPKPGQHDSKPGVAWKTVGGTVKEIHGETYTVEDFDGSQM
                     KVHVGQGTKHLRGNKKVGDTIRAEITHGGFANSIQ"
     gene            complement(913..1650)
                     /locus_tag="PKK81_08080"
     CDS             complement(913..1650)
                     /locus_tag="PKK81_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNV32872.1"
                     /translation="MMRRGIVLLMCLSGAAMVAHAQGSAKLEVGHFSVAAEGAVLPDG
                     WAPLTFKKIERHTTYEVVKDGPVSVVKAVSEVSASGLTKAVAIDPHEYPIVRWRWKVE
                     NLLQKGNVNRKDGDDYPARLYITFAYEPAKVSFGRKLKYQAGRVLFGDIPIGALNYIW
                     DGKSPVGTVVDNAFTDFAKMIVVESGPQRIGTWVEEERNVYEDYRLAFGEEPPAISGV
                     AIMSDTDNTKERAVAYYGDIVFVRALK"
     gene            complement(1659..3212)
                     /locus_tag="PKK81_08085"
     CDS             complement(1659..3212)
                     /locus_tag="PKK81_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HNV32873.1"
                     /translation="MSASVQSLVLPDDEHNRMLVANVHPPDWLNPEPAGRYNMVVVGA
                     GTAGLITAVVAAGLGAKVALIERHLMGGDCLNVGCVPSKALIRAANAWAQLRDASAFG
                     LHIPPGVTHDFGAVMTRMRKLRAGISHVDSAHRYKSLGVDVYIGQARFTGRDAVSVEG
                     PSGNRTLTFVNAAVCTGARASAPAIPGLAEAGYLTNETVFSLTELPARLAVIGAGPIG
                     CELAQAFARFGSEVSLIEAMHGIMPNEDRDAAEVVQQSIMRDGVRLLCCGKDLRVERT
                     ATGKRLLVDSHGQHYDVTVDEILVGVGRSPNVEGLGLETIGVEYDKTGVKVNDRLQTT
                     NPRIYAAGDICSRYKFTHAADAMAQIVIQNALFPHPFGLGYASMESLIMPWCTFTEPE
                     IAHVGLYEADAKKKGLEIETYTYKLGEVDRAILDGEQEGFARIHIQKGTDKILGATIV
                     AAHAGDLISEFSVAMKAGAGAKTIAATIHPYPTRAEVNKKVVNLWRKAHFTARTKMLL
                     TRLFAWLRR"
     gene            complement(3270..4016)
                     /locus_tag="PKK81_08090"
     CDS             complement(3270..4016)
                     /locus_tag="PKK81_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HNV32874.1"
                     /translation="MTTSVESPSSSKRAGLGKLMLGIFVGLSLAAFFYFDLGQYLSLD
                     GLKSNRARLLAFTEANYPVAVAMFVLGYCVVVGLSLPGGAIMTLAGGFLFGSLLGTLY
                     VNVGATVGATLAFLVARYLLREWVEQKFGSRLDAIQQGFARNAFSYLMTLRLIPLFPF
                     FLVNMVSGLTRVNVGTYMAATSLGIIPGSFVFAYAGRQLGTISSLKEIASPNVLLAFT
                     LLGLLALVPILYRKLAGKPVSLQDGSRRHP"
     assembly_gap    4072..4171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4462..4611
                     /locus_tag="PKK81_08095"
     CDS             4462..4611
                     /locus_tag="PKK81_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32875.1"
                     /translation="MGPLSVHDMKSGFFLFASFLLLATICAIAALGTLMRPQRWSERE
                     TRLHV"
     gene            4860..5504
                     /locus_tag="PKK81_08100"
     CDS             4860..5504
                     /locus_tag="PKK81_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HNV32876.1"
                     /translation="MDQNDRFIKTKENWAKARRGGEEREVFYEGDDRLPPGQHLVETW
                     PVLDLGQKPDIPLSEWTLTIGGAVTTPATWTWADFLAQPQFKNVSDFHCVTSWSRYDN
                     EWEGVSFKQLMAVVQPLPSAKFILFKSYDDYTTNLPLVACQDDDVLLTYKWNGRPLTK
                     EHGGPVRMIIPKRYAWKGAKWIKEITFSDHDEKGFWEVRGYSNSAFPWQNDRYG"
     gene            5501..6622
                     /locus_tag="PKK81_08105"
     CDS             5501..6622
                     /locus_tag="PKK81_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNV32877.1"
                     /translation="MNEHNSPVNAPIIQRIEIWPVDIPITDPFVVATGARVTAQNLFM
                     RVTLRNGTQGVGEAAPFPEVGGEDRATCFAAATELAKTMIGQSVGDYESLADRLNEQA
                     TLHPAARCGLETAMLDAYCRSRRIPLWQLWGAADVRERETDITIPICSPEKTLELARG
                     WYARGFRLFKMKVGTDVEQDIRRLQAVHNALPEIGFIGDGNQGFSREDCLRFVHGVKQ
                     FGGRLVLLEQPVVRDDLEGLQAIRHLTGIPVAADESVRSLDDAREVVRMQAADYINIK
                     IMKTGVIDAWRIAAFTRSAGLRLMVGGMLETRIAMGCSFSLVLGLGGFDVLDLDTPLL
                     LSTDPVTGGYRYSGPRLQPWSEGGLDMQIPCPADRITVQ"
     gene            6814..7617
                     /locus_tag="PKK81_08110"
     CDS             6814..7617
                     /locus_tag="PKK81_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32878.1"
                     /translation="MQVITPRPILATASPRNIRPIALFWMLGVGTLMLGGCVSQQTYE
                     TARHEVKTRATELAQTQADIQSLEQQREETHLANQREERSLATLKGELKKIQLAYEQL
                     HKSNQAKLAALEYSIVALRARHQAMLKEISETKRIEKRLEALTAQHEHTMATTPSGPE
                     ARVTTVDGSPQEPHLVAVITPQTPQIDSTPTASQAPPAATSAATTPAVPQPAPVVATP
                     PSPSTPAKAAQAPAAPQAPRPATAAAPQDESWFSSVTGWFTSLFDWIWA"
     gene            complement(7691..7912)
                     /locus_tag="PKK81_08115"
     CDS             complement(7691..7912)
                     /locus_tag="PKK81_08115"
                     /inference="COORDINATES: protein motif:HMM:NF023510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HNV32879.1"
                     /translation="MQQAIIGYHQDDEGHWVADLRCGHGQHVRHQPPMTSRPWVLTEE
                     GRRAFLGTELNCKKCEEGGDGFDPTGAQS"
     gene            8054..8953
                     /locus_tag="PKK81_08120"
     CDS             8054..8953
                     /locus_tag="PKK81_08120"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV32880.1"
                     /translation="MAQSEFRIGIVGIGRMGANMARRLHDLHYAIVAVYDTDTERAGA
                     LAKELACTAVTTPARVAELADTVLTVVSDDAAMRQIYSLGGPDSLLRHAANRLFINCA
                     TLSPELQREVHALVEQQGGRILEACMASSITQARQGTLYLMCGGRPEVFERAKPLLQD
                     LSAHLRYIGPAGEAAKVKALVNMVMNANTAALAEGLGLGSALGLDLTMLREVFSQTGA
                     NSRVLETDGEDMQQRAHDCYFSAAHAAKDSGIACALAQQAGLDLPLAKATYDQYRRLI
                     DHGKGELDKSAVAELTFKDRLNH"
     gene            complement(9063..9527)
                     /locus_tag="PKK81_08125"
     CDS             complement(9063..9527)
                     /locus_tag="PKK81_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32881.1"
                     /translation="MMRGRGRGPGWALIGLVLLLSACNSDRPEPAEFDLPSAGLRLNL
                     TRLATHPFLSRYRLTLRIEGPRGCSVTAELFPDTGYAGRRNVYQQPSGVITVLGQYDA
                     RVFDPANCSLRLVEFQSLVGQATFLGAFDVDAQKRWRFLDASVRPERSFEKH"
     gene            complement(9524..10075)
                     /locus_tag="PKK81_08130"
     CDS             complement(9524..10075)
                     /locus_tag="PKK81_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNV32882.1"
                     /translation="MVGTIQSLKRGLGRVGMMAGVLFLAGGWPVVASAADELPKEAVL
                     PAALAGKAAQAALDFCKKDGYRVSASVVDRAGVLRAMMRADGAGPHTVDSSRKKAYTA
                     ASLRRATSDLADMIAKQPALQALREMNESILMVGGGLPIEIAGEVVGAIGVGGAPGTH
                     LDDACAEAGLDAIGAASKMPTAK"
     gene            complement(10219..>10534)
                     /locus_tag="PKK81_08135"
     CDS             complement(10219..>10534)
                     /locus_tag="PKK81_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32883.1"
                     /translation="QVGGEYGALFPAIPPTGRYPKQDVLDQLINDTPLPSVSCVGPAG
                     SVILCDASGLHRGGRSLTHPRIVLVGVYTSNAGLDAARYSLPRSVGHEQLSPAAKFAL
                     YS"
BASE COUNT         2244 a         3437 c         2842 g         1911 t
ORIGIN      
        1 ccaaagatgc cccggatctc ctctcgcgtc agttcatatc gtccctggtt cagaggaaga
       61 tcgcagtagc cacaggcgga gttgcaacga agggtcacct gaaacgtggc caggaccggc
      121 cgccctcgca cgagattagc caacggccgg gcacagtcgg caatcatggc cagcgaaggc
      181 ttcctcgtca tcgtggattc cccggtcggc ctgagcatat ttgaacacct gttgccattt
      241 ccatccggcc ccgccgcccc cggttcagca caacgcgaca caatcggcac caatccaggc
      301 gattctgcac tgcaggcaac cggtttcctg gcatacggct tgctgaatca ctctctacac
      361 tgcagcctga ctcatcccag ggaggatcgt tatgaacacc aagctccagg cctcgatact
      421 caccatcgcc gcggctagcc ttgcaaccgt cgcttttgtc gtggcgccgg catcggcaaa
      481 ggacaaggag aaggccggca aacattcgaa ccatgcgacc accacctcgg cagccgcggc
      541 ctcctcactt ctgcgatcag tccctgagca acccccggca ccgaaacccg gacaacatga
      601 ctccaaaccc ggagtcgcct ggaagaccgt gggcggcacc gtcaaagaaa ttcacggcga
      661 gacgtacaca gtcgaagact tcgatggcag ccaaatgaag gtgcatgtcg gacagggaac
      721 caagcacctc cggggcaaca aaaaagtggg cgacaccatt cgggctgaaa ttacccacgg
      781 tggcttcgca aattcaattc aataaccccc tgactctgcg aacccacatc agctcgccgg
      841 tgtcctggag aggccgaccc tgcacaggtc ggcctccccg cctttctcag ttccggcagt
      901 tccccagccc cgtcacttca acgccctgac gaagacgatg tcaccgtaat aggccacggc
      961 ccgctccttg gtgttatccg tatcgctcat aatcgcgacg ccgctgatcg ccggcggctc
     1021 ttcgccgaag gccagtcgat aatcctcata cacattgcgc tcttcctcga cccacgtgcc
     1081 gatgcgctgc ggcccgctct ccaccacgat catcttcgca aagtcggtaa aggcattgtc
     1141 caccaccgtc ccgaccgggc tcttgccgtc ccaaatgtaa ttcaatgcgc cgatcggaat
     1201 atcaccgaac agcacccgac cggcctggta cttcaatttc cgtccgaaac tcacctttgc
     1261 cggttcatag gcaaaggtaa tatagaggcg cgcgggatag tcatcaccgt ccttgcggtt
     1321 cacattgccc ttctgcagca gattctctac cttccatcgc cagcgcacga tcggatactc
     1381 atgcgggtca atcgccacgg ccttggtcag gcctgaggcg gacacctcgc tcacagcttt
     1441 caccacggaa accggcccgt ccttcaccac ctcgtatgtc gtgtgccgtt cgatcttctt
     1501 gaacgtcagc ggtgcccacc catcgggcaa tacggcgcct tcagccgcga ccgaaaagtg
     1561 tccgacctcc agcttcgcag agccctgcgc atgtgcgacc atcgcagcac ccgacaagca
     1621 catgagcagg acaatcccgc gccgcatcat gtgtcccctc atcgcctcaa ccaggcaaag
     1681 agcctcgtta acagcatctt agtccgcgcc gtaaagtgcg ccttgcgcca gaggttgacg
     1741 accttcttgt tgacttccgc ccgggtggga tacggatgga tcgtggcggc gatcgttttc
     1801 gcaccggcac cggccttcat cgccacagaa aactcactga tcaagtcacc ggcatgcgcc
     1861 gccacgatcg tggccccgag aattttgtcg gtgcctttct gaatatggat gcgggcaaac
     1921 ccttcttgct caccatccag gatggctcga tccacctcac cgagtttgta ggtataggtt
     1981 tcgatctcca agcctttctt tttggcatcc gcttcgtaga ggccgacatg agcgatctcg
     2041 ggctcggtaa acgtgcacca gggcatgatc agcgactcca tgctggcata ccccagcccg
     2101 aagggatgcg ggaacaatgc attttgaatg acgatctgcg ccatcgcatc cgcggcatgg
     2161 gtgaatttat accgggaaca aatgtcgccg gcggcgtaga tacgcgggtt cgttgtctgg
     2221 aggcgatcgt tcactttcac gccggttttg tcgtactcaa ccccgatcgt ctccaacccc
     2281 agcccctcga cattcgggct acgcccgacg ccaaccagaa tttcgtccac cgtgacatcg
     2341 tagtgctggc cgtgggaatc aaccagcaac cgcttcccgg tggccgttcg ttccactctc
     2401 agatccttcc cgcaacagag cagcctgaca ccgtcccgca ttatggactg ctgcaccacc
     2461 tccgctgcgt cccgatcctc attcggcatg atgccgtgca tcgcttcaat gagtgacacc
     2521 tcgctcccga agcgagcgaa ggcctgcgcc agttcgcacc cgatcgggcc cgcgccgatc
     2581 acggcgagac gcgccggcaa ctccgtcagc gaaaacaccg tttcattggt gagatatccc
     2641 gcctctgcca accccgggat ggccggcgcc gaagcccttg ccccggtaca gactgccgcg
     2701 ttgacgaagg tcagcgtccg atttccagat gggccctcca ccgacactgc atcccggccg
     2761 gtgaagcgag cctgcccgat atagacatcg acccccagcg acttgtagcg atgagcggaa
     2821 tccacatggc tgatcccggc acgcaatttt cgcatcctgg tcattaccgc accgaagtca
     2881 tgagtcaccc ccggcgggat atgcagcccg aaagccgagg catcccgcaa ctgcgcccaa
     2941 gcattggccg cacgaatcag cgccttggag ggcacgcatc cgacgttcag acaatcgcct
     3001 cccatcagat ggcgttcgat caacgcgacc tttgcgccca atcccgccgc caccaccgcc
     3061 gtgatcagcc ccgcggtgcc ggcaccgacc acaaccatgt tgtagcgacc ggcaggctcg
     3121 ggattcagcc agtccggcgg atgcacattt gcgaccaaca tccggttgtg ttcgtcatcc
     3181 ggcaggacca agctttggac agaagcgctc attgaagtcg gattctccct ctggtgaata
     3241 accatggacg cggtctttcg ccctgaccgc tatggatgtc gacgagaacc atcctgcagt
     3301 gagaccggct taccggccag cttcctatag agaataggca ccagcgccaa cagaccgagt
     3361 aacgtgaacg cgagcaacac attgggcgag gcgatttcct tgagtgaact gatcgtgccg
     3421 agttggcggc cggcatacgc aaagacaaaa ctgccgggaa tgatgcccaa ggaggtcgcc
     3481 gccatgtagg tccccacatt gaccctggtc agccccgaga ccatattgac cagaaaaaac
     3541 gggaacagcg ggatcaaccg cagcgtcatc agataactga acgcattcct ggcaaatccc
     3601 tgctgaatcg catccaaccg gctgccgaat ttctgctcca cccattccct gagcagatag
     3661 cgagccacca aaaacgccag cgtcgcaccc acggtcgcac cgacattgac gtagagcgtg
     3721 cccaacaggc tgccgaagag aaatcctccg gccaacgtca tgatcgctcc accgggcagc
     3781 gagagcccga ccaccacaca gtagccgagc acgaacatgg ccaccgcgac cggataattg
     3841 gcttctgtaa acgcgagcag acgagcccgg ttggatttga gcccatccag cgagaggtac
     3901 tgtccgagat caaagtagaa aaatgcggcg aggctcagcc cgacaaaaat ccccagcatc
     3961 agtttgccga gcccggcgcg tttcgaagat gagggcgatt ctacagatgt ggtcatggca
     4021 ccttggggcg gtatcatcct gcctcctcac tcatgtgtca atcgtggact cnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttgtccct ccgaaagaac tcttgaagac
     4201 agggtgccag tggaggtaat gtctcagatg catgcgctga ttgaactctt ggactcggct
     4261 atgctgacaa tacactcggt ctcacagcag tcccagaact ctcaccccgt ctatgagacg
     4321 acttcacatg tgtggatgcg cccctgccct cactgctcct cgcgcctaag aaagaaatca
     4381 cctgaagaat catggcgttg cggatgcggt tggcacgcag agtgaagtgg tacaattaga
     4441 tcattgtaag ggggtgacgt catgggaccg ctctcggtgc acgacatgaa aagtggattt
     4501 ttcctcttcg caagcttcct gctcctggca acgatctgcg ccatcgcggc cttgggcacg
     4561 ttgatgcgtc cgcagcgctg gtctgaacga gagaccagac tgcacgtctg atcctcgcca
     4621 agagaaccaa cggccaggct atacgcaggc cgggggtgca cctgtgccgt ccgatcactc
     4681 gaatcactct ctcctgacca tccgcctctt cacactcaat ccgaaggccg aactccccga
     4741 caatactgga cttcttcatt tcttagcgga caggtcacgg agcacccgcc acccgatcga
     4801 aagtaccggc cttgtaagat cctggcctct tgccctactc tgtctccagg aggctgggta
     4861 tggatcagaa tgaccgtttc atcaagacca aggaaaattg ggccaaggcc cgacgcggcg
     4921 gggaagaacg cgaggtcttt tacgaaggag atgatcggct ccctccaggc caacatctcg
     4981 tggaaacctg gccggtactc gacctgggcc aaaagcccga tattcccctg tcggaatgga
     5041 cgttgaccat cggaggtgcg gtgaccacgc ctgccacttg gacctgggcg gacttccttg
     5101 cgcaaccgca attcaagaac gtatcggact tccattgcgt gacgtcctgg agtcgttacg
     5161 acaatgagtg ggaaggggtc agcttcaaac agctgatggc tgtcgtccag ccgctcccgt
     5221 cggccaaatt catcctcttc aaatcgtacg acgactacac cacgaattta ccgctcgtcg
     5281 cctgtcagga cgacgacgtg ctccttacgt acaaatggaa cggccggccg ctcaccaaag
     5341 aacacggcgg tccggttcgc atgatcatcc cgaaacggta tgcctggaaa ggcgccaagt
     5401 ggatcaagga aatcaccttc tccgaccacg acgagaaggg gttctgggaa gtgcggggct
     5461 attcgaacag cgccttcccc tggcagaatg accggtacgg atgaacgaac acaattctcc
     5521 ggtgaacgca cccatcatac agcgtatcga aatctggccg gtcgatattc cgatcacgga
     5581 tccgttcgtg gtcgccacgg gggcacgggt gacggcgcaa aacctgttca tgcgagtcac
     5641 cttgcggaat ggaactcagg gtgtgggaga agccgcgccc tttcccgagg tcggaggtga
     5701 agatcgggca acctgcttcg ccgcagccac ggaactggcg aagactatga tcggccaatc
     5761 ggtcggggac tacgagtccc tggcagaccg gctcaacgaa caggccacac tccaccctgc
     5821 cgctcgttgc ggcttggaaa cggcgatgct cgacgcctat tgtcgatcac ggcgcatccc
     5881 gctctggcaa ttgtggggag ccgcggacgt ccgcgagcgg gaaaccgaca tcaccatccc
     5941 gatctgcagt ccggaaaaga cgctggaact cgcacgtggc tggtacgcca ggggattccg
     6001 cctctttaag atgaaagtcg ggacggatgt ggaacaggac attcgccgat tacaggccgt
     6061 gcacaacgcg ctccccgaga tcggatttat cggcgacggg aatcagggat tctcccgtga
     6121 ggactgtctc cgctttgtcc atggagtgaa gcagttcggc gggcggctgg tcttgctcga
     6181 gcaaccggtc gtgcgcgatg atctcgaagg actacaggcg attcggcacc tgacgggcat
     6241 tcccgtggcg gctgatgagt ccgtccggtc gctggacgac gcgcgcgagg tcgtccggat
     6301 gcaggccgcc gactatatca acatcaagat catgaaaacc ggcgtgatag acgcgtggcg
     6361 aatcgccgcc ttcacccgct ccgccggcct gcgtctcatg gtcggcggca tgctggaaac
     6421 ccgtatcgcc atgggctgct cattcagtct cgtgctgggc ctcggcggct tcgatgtgct
     6481 ggatctggac acaccgctcc tcctctcaac cgacccggtg accggagggt atcgatatag
     6541 cggcccgcgt ctccagccct ggtctgaagg cggactcgat atgcagatcc cttgtcccgc
     6601 agaccgcatc accgtccagt agcacggatc cggacctcct cacgcaacct gtttgctctc
     6661 tgattctcac ccccgacaac gcactgcagg tttcccgaaa tattttgccg tttttccact
     6721 tttctgaaaa attaacctca ggcacagcgg cttcgcacag gcggagcacc gatcgcaacg
     6781 actggcaccg tacgtacccg ggaggaaggt cccatgcaag taatcactcc gcgcccgata
     6841 ctcgcgaccg cgtctcctcg caacatccgt cccatcgcac tcttctggat gcttggagtc
     6901 ggtaccttga tgctgggagg ctgtgtttcg caacaaacct atgagacagc caggcatgag
     6961 gtaaaaacgc gggccactga actcgcccag actcaggcgg acatccagag cctcgaacaa
     7021 caacgagaag agactcacct cgccaatcag cgtgaggaac gctctcttgc cactctcaag
     7081 ggcgaactga aaaagatcca gctcgcctac gagcagcttc acaagagcaa ccaggccaaa
     7141 ctcgcggccc tcgagtacag catcgtggca ttacgcgccc ggcatcaggc catgttgaag
     7201 gaaatcagcg agaccaaacg gatcgagaaa cggctggaag cgctcacggc ccaacacgaa
     7261 cacacgatgg cgacaacacc atccggcccg gaggcccgtg tgaccacggt ggacggaagc
     7321 ccgcaggagc cgcacctggt cgcggtcatc accccgcaaa cgccacagat tgattccacg
     7381 ccgaccgcat cgcaggcgcc cccagccgcc accagtgccg ccacgacacc ggcggtgcct
     7441 cagcccgcac cggtcgtcgc aaccccgcct tcacccagta ctcctgcaaa ggccgcgcag
     7501 gcacccgctg cgccacaagc cccgcgaccc gcgaccgcag cagcccctca ggacgaatcc
     7561 tggttctcaa gtgtgacggg atggttcacg tccctcttcg actggatctg ggcctagaga
     7621 tcgtcctcaa cgcctctctc catcaattct gatccaggcg tttcatcgcc ttatcagccc
     7681 accggagccc tcaagactgg gccccggtgg ggtcgaaccc gtcgccccct tcttcacact
     7741 ttttacaatt caactccgtc ccgaggaacg ctcgccgccc ctcctccgtc aatacccacg
     7801 gacgactcgt cataggcggc tgatgtcgca catgttggcc atgaccgcag cgaaggtcgg
     7861 ccacccaatg gccttcgtcg tcctggtgat acccgataat cgcctgttgc ataccggact
     7921 ccccgtgttt cgtgccgctg ttttgaattg acgcactccc ctgacagaac cggacgcgtg
     7981 gatacctgca tccaaaggga ctgtggtaga cttccgcgcc cggcagagta gcatgttttc
     8041 caggaggttg cggatggcac agtcagaatt tcgaatcggc atcgtgggaa taggacggat
     8101 gggggccaac atggcccggc ggctacacga cctccactat gcgatcgtgg cggtctatga
     8161 caccgacacg gaacgcgcgg gggcgctcgc gaaggaactc gcgtgcacgg cggtcacgac
     8221 tccggcgcgt gtggctgagt tggccgatac ggtgctgacg gtggtgtcgg acgatgccgc
     8281 catgcgccaa atctattcac tcggcgggcc agacagtttg ctacgccacg ccgccaatcg
     8341 cctgttcatc aattgcgcca ccctgtcacc tgagctccaa agagaagtcc atgcgttggt
     8401 cgagcaacag ggcggccgaa tcctggaagc ctgcatggcc agcagcatca cacaagcgcg
     8461 ccagggcacg ctctatttaa tgtgcggcgg acgaccggag gtgttcgagc gggcgaagcc
     8521 gctcttgcag gatctgagcg cacacctgcg gtatatcggg cctgcgggcg aagccgccaa
     8581 ggtgaaggcc ctggtaaaca tggtcatgaa cgccaacacc gccgcgctgg ctgaaggact
     8641 cggactgggg tcggccctcg gcctggacct cacgatgctt cgagaagtct tttcacagac
     8701 cggcgccaac tcccgcgtgc tcgaaaccga cggtgaggac atgcaacagc gggctcacga
     8761 ctgctacttc tcagccgccc acgcggcaaa agactcgggg atcgcctgcg cattggccca
     8821 acaggcaggc ttggacctgc ccctggccaa agccacctac gatcaatatc gacgtctcat
     8881 cgaccatggt aagggagaat tagataaatc tgccgtcgct gagctgacct tcaaagaccg
     8941 gctgaatcat tgacgatctg acgccccaca cattctaccg atggaagcag cgccccggtc
     9001 acagccgcac cgaccaccct cgcagtagtt cacgaagccc gcgctcgccg gtccgctccg
     9061 ggctaatgct tttcgaacga ccgctccggt cgcacggacg catcaagaaa tcgccaccgc
     9121 ttctgggcat cgacatcgaa cgcgccaaga aatgtggctt ggccgaccag cgattgaaac
     9181 tccaccaggc gaaggctgca gttggccgga tcgaacaccc gcgcatcgta ctgccccaag
     9241 accgtgatga cgcccgacgg ctgttggtag acattccgcc gcccggcata ccccgtgtcg
     9301 ggaaacaatt ccgccgtgac tgagcagccg cgcggccctt caatgcgaag ggtcaatcga
     9361 tagcgggaga gaaaggggtg cgtggcgagg cgagtcaggt tcagccgaag cccggccgag
     9421 ggaagatcga actcggccgg ctcagggcga tcactattgc aggccgacag gaggaggacc
     9481 agaccgatca gagcccagcc cggtcctctc cctcgtcccc tcatcatttg gccgtaggca
     9541 tcttcgaggc cgctccgatt gcatcaaggc ccgcttcggc gcaggcatcg tccagatgcg
     9601 taccaggcgc gcccccgaca ccgatcgccc cgaccacctc gccggcgatt tcgatgggta
     9661 acccgccacc gaccatcaga atactctcat tcatctctcg caatgcttgc aatgcgggct
     9721 gcttggcaat catgtcggcc aagtcgctcg tcgcgcgccg caagctggca gcggtatagg
     9781 ccttctttct gctgctatcg acggtatgtg gaccagcccc gtcggcccgc atcatggccc
     9841 gcaagacccc tgcgcgatct acgaccgacg cactgacccg atagccgtcc ttcttgcaga
     9901 aatcgagcgc cgcttgcgca gctttacccg ctaatgccgc cggcaacaca gcctctttgg
     9961 gtaattcatc cgccgccgac gccaccacag gccacccgcc ggccaggaac agaacccccg
    10021 ccatcatgcc gacacgcccc aggccccgtt tcaacgattg tatcgtcccc accatcacag
    10081 gcctccttcg ttctctatcc cctgcgtagt caaccgaaca cccctatccg tattccgacg
    10141 tgcgagggta cggccatcaa accgctctgt caatactcag cgctcaagct ttcagatatg
    10201 cgaacaggta ccggcgtgct aggaatacag ggcgaacttc gcggcagggg acaactgttc
    10261 gtggccgacc gatcgaggga gggaataacg ggccgcatcg agtccggcat tggaggtgta
    10321 aacgcccacc aggacgattc gcggatgcgt aagcgaccga cctccccggt gcagcccact
    10381 cgcatcacac aggatgacac tgcccgccgg cccgacgcag gagacagacg gcaacggcgt
    10441 atcgttgatg agttggtcga gcacatcctg tttcgggtac cgaccggtcg ggggaatggc
    10501 aggaaagaga gccccgtact caccgcccac ctga
//
LOCUS       DAOQRM010000084        10522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_5587_length_10522_cov_13.906372, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000084 DAOQRM010000000
VERSION     DAOQRM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5587_length_10522_cov_13.906372"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKK81_08140"
     CDS             <1..987
                     /locus_tag="PKK81_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32884.1"
                     /translation="GLSILWPACWTALPIKMAFAMLFMAMGTIHLETKLGITFLMLLM
                     TPVSVFAFFVISLGVGFHFGEGIGLPLLFLLSIPIDIWSLGLVARTVFLERLRLEPPD
                     SLGIGLWVRFAVAGAIYLPFLWVVQGGATDLARSITKSILDMDMLKAMPVAERIGLEF
                     TAWGSAAFVVLLALTFVGLSILGKLVEGRAAGAQPAGDTYQDLISRWDMIRVPADQSL
                     MLTAFTAAGAVLSLLFWAVLPVSTPHPHECCKPATVEAAPPFDAQKSLSKGEKAFKDA
                     ELKIAAIEQRTAEDEKDKQKGGKDKAGVKDGSPKSDGKPSQSAAGKPAGSGK"
     gene            1016..2896
                     /locus_tag="PKK81_08145"
     CDS             1016..2896
                     /locus_tag="PKK81_08145"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNV32885.1"
                     /translation="MMNQGQIVTNHGSQIAGRVALLAGCAALLAWVGMPDAALAQEAA
                     PQAPTAYRDIPYIGSRNLVWIIAQLHLLLAGFVLGVPIFAWLCEIVGWKSGDKRYDKL
                     AKEFTKLLTSSYATTALFGGILLFLLIGFYPKLMAYLTDIFFPSFLVYCGLFLVETAT
                     LYLYWYGWDAMAEGSGKKFHIFLGFLLNVFAFFIMIVPNSWATFQSSPVVISEGSDFA
                     RAWAATWNPTWWPVNIHRLIANVVLGGYICGAYAGIRYLSAKSQEERYHYDWMGYVGN
                     FIGVFGLLPLPFAGYWLMREIYQYNQQMGITLMGGFLSWLFILQAMLIGVLFLGSNYY
                     FWLGITHRIPGSETQYRKPIMAMLIILLLCLGVWMTPHSLVASLEEAQKMGGTHHPLL
                     GVFGVMSAKMTVSNLMVLVTFMSFIMYWRAGKQDTAGWAKAAKALMGGVLGLAAIAVI
                     VLGVWGYFVPAIIRINYFSTSQVGIVIFVMLTITPLTALLLKSAKTTTEMVWGRMPPR
                     AGYALVLNAVMVILLMTLMGYARSSSRVHWHIYGVMRDSSQYAFSPALGYAAALMALN
                     TFLFCMLVAFIFWVATMGDKAKAAASTKGSVEPGAIPAMAGGSPALDGPEADASEEKR
                     PV"
     gene            2963..3583
                     /locus_tag="PKK81_08150"
     CDS             2963..3583
                     /locus_tag="PKK81_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV32886.1"
                     /translation="MSEVVKLQLIGLCVVGLGTVILLFIKGQFIRVIGFVAIVLGLFS
                     LVALAVPQMASLPPAEESINIADIKTPTDMATIGQKIFFSKGQCALCHSIGPSESARC
                     PDLKGIGAKLSREFIFESLTEPQAYLYLDYRHEGVPKEYPARMPYINKNPIGLSKNEI
                     LSVIAFLQQMSGEPISVSPTELDLPRATPAPVKAADAGTLAVAQAR"
     gene            3611..4336
                     /locus_tag="PKK81_08155"
     CDS             3611..4336
                     /locus_tag="PKK81_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV32887.1"
                     /translation="MGGVLVRPIILTVCIYLFLKFIVPNLPHSAPLPSSLIFLYLMLT
                     TAGIVIFATLSGASKDAFFGPILRFLTGEGGGALSGARYLVLALFPLLVGWQAYGSTA
                     SSDAPPGENRTIHPAPPGEYTGLSNPVPKTLDNINYGKGLYASRCSPCHGNFDGKGFA
                     AAGFTPPPANFKDPTTIAMLQESYLFWRIKKGGVGLPVEGMPWKTAMPRWELEMSDED
                     IWKVIMGEYDGAGQKPRTWDESE"
     gene            4443..5204
                     /locus_tag="PKK81_08160"
     CDS             4443..5204
                     /locus_tag="PKK81_08160"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNV32888.1"
                     /translation="MAAEVQPAYSQQSVSVRAALVKGSLPADDPTAAAWATAAVSEFP
                     MSPQVHWPVRITDVTAKSVKVRGLHDGTTVAILLEYSDPSEDPEDAAALEFMVGDTKA
                     HFAHGQPMAQVEGGPVNIWYWKNKDGKGADLGAKGFGTLKPHAHQDVKAKGVYQGGVW
                     KVVFSRPLSTEHVAEDTQFKPGTFASIAFAVWDGKKMETGQPKEKGSEKAISSWWYFR
                     ADAPPDYSPYMYALLAVALALGFELVLVRRLKKGS"
     gene            5294..6220
                     /locus_tag="PKK81_08165"
     CDS             5294..6220
                     /locus_tag="PKK81_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV32889.1"
                     /translation="MFQNEQSAKGKKLYAHYCMHCHGENGRQNEGFNWSSMTDPKPKD
                     LSNKDEMGTFKDEEIFNTISRDMKDTGPGGEKIGDDEFAVPTMPTFKYTLSEEEIWGI
                     VGYVRSLHGKKLEFNVEGRKKDLQAAQQAADQKFKEAERVAQEAEKKASDEADKKGVE
                     VDDNAYAKEMQAMGQAKKELDQATAALANFTTRPGKGVNIARPDLNMKPDAAAKLVEV
                     GKQIYVTKYGCNGCHKIGDEGGKVGPALDRAGYRLNPTWVYRWLRNPQAMKPDTRMPS
                     LGLNDADAKAVALYLKTLRAPKPDQPLGKVGE"
     gene            complement(6225..7070)
                     /gene="ubiA"
                     /locus_tag="PKK81_08170"
     CDS             complement(6225..7070)
                     /gene="ubiA"
                     /locus_tag="PKK81_08170"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNV32890.1"
                     /translation="MADLIRLRNQSGTWLLLLPSLWSLVLANQGRPPMWLLGVFTLGA
                     FVMRSLGVVFNDLADRNFDKHVARTRTRPLAAGRLAPRQALLVALGLAVIAAALVITL
                     NPFTMLLSPIALFLAAIYPFSKRWIQIPQGVLGIAFGWGAVMAWAASRGSIEAPAWWL
                     FAATTCWAIAYDTIYALQDCEDDRRIGVKSSALLFGEAVPVAVGVFLVGMVLCLVVAG
                     QLSGLGMGYYLILALPTGVFLWQVRRLQAPVTPHIAFAMFKQHVYSGVAILVGIWIGT
                     FNQTP"
     gene            7306..9198
                     /locus_tag="PKK81_08175"
     CDS             7306..9198
                     /locus_tag="PKK81_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32891.1"
                     /translation="MSSGCQFFGQRSSLATHYNLPLTVQLRMDHSIAAASLDYRDACG
                     QALTLPIRDGLETQLKKRMGQVFERVHLEPGPSTGAVDGVVDTALGVRQVDLFIPRKA
                     NKSYPATVTLGMEFSYTDHHGTVLVSKKLQSAATGEVETRADTCNISGLDKVAEEAAA
                     KLVEGMAQQLGTATKIREQAQFRKSGDKGTMQSAPVPPATPAVAPLAATPVVPVAPLQ
                     SAAGSTPQAAAPDSAIRSGMTQLSFRTIIRDDNQNHVLEQRESFSVEFEVKNEGAVVA
                     EGVEVELSGHAAIVGGLKSPVSLGTLQPGEVRRVAVDGKVGAVADAEQAELMCALRAS
                     SNVLLPSAKKFFVAIHPDAGAAVEVLSVDVDQLPSRNGKSAQPNSVGIAIGIGAFRDP
                     AVPTVKFAAHDADVMGGYFKTVLGVPPHKVKVLSDAKALRDDLIEVFEQWLPKQGSPQ
                     ATVYIYLSGRAVVDQETGIVSLLPYDGTRAGASRMFSLARLQRALTKASVKQAVLMLD
                     LSLEPSSGSDPGRVVPPRWTQQDSSGEPDRVMLMIGNSGLQEAQAYQPGQHGLFTYFL
                     LKGLRGAADLDKSGTVLSGELCEYVHDQVDAVTRGATGGGQPTLCLPMGGRQPVLTGI
                     PLSKIR"
     gene            9232..10383
                     /locus_tag="PKK81_08180"
     CDS             9232..10383
                     /locus_tag="PKK81_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNV32892.1"
                     /translation="MLTGRTRWPRARRILFLACLWLASVSCSIIVLEWVTGYLYGRTP
                     FSNGKRIIDLYLGQQPTVRSDVNGESASLASHPYLLYTHLPNLTTDGYQQTNSLGYRN
                     REFTVAKPPDTIRILVLGGSTTFMWPFVKNPADTWVARLEAKLKTISKKTVQVVNAGL
                     NYGTSAEALAGYVFRHRFLQPDIVIYHGGGNDVLPLFFGNYTPEYTHFRRYGAGEIPR
                     PGEKFLLQHSSTAKYVYSRWLEPIGAVVVTEPFWEVDPKTALERVRMQAPEGFRRNLD
                     MLVSSSQQAGSAVVLFGFLQARRQNLSKNAGAFKGFEEALILGLEKNYDVMREIATRY
                     RVPFVIPPQDRFEDEWFQDNCHLSPAGEEVKAQIMFEQLRDHPIFLSAL"
     gene            complement(10334..>10522)
                     /locus_tag="PKK81_08185"
     CDS             complement(10334..>10522)
                     /locus_tag="PKK81_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32893.1"
                     /translation="RPAVYETAALPTELRRLVDLNIIFGSVKERNLTQGMEALSMIET
                     PPVTEQIEKWDDPVIVRT"
BASE COUNT         2198 a         2802 c         3230 g         2292 t
ORIGIN      
        1 gggcttagca ttctctggcc ggcctgctgg accgccctgc cgatcaagat ggcgtttgcc
       61 atgttgttta tggcgatggg gacgatccat ctggagacga aactcgggat tacattcctg
      121 atgttgctca tgactccggt cagcgtcttt gcattcttcg tgatctccct aggtgtcggg
      181 ttccatttcg gggaagggat cgggcttccc ttattgttcc tgctctccat tccaatcgac
      241 atttggtcct tgggcttggt cgcccgtacc gtgtttttgg agcgcttgcg gctcgagccc
      301 cccgactcac tgggcattgg gctgtgggtt cgatttgcgg tcgccggggc gatctacctt
      361 cccttcttgt gggtggtaca gggcggcgcg accgatctcg cccgttcaat tacgaaatcc
      421 attttggata tggatatgct caaggctatg ccggtcgctg agcgaatcgg tctggaattc
      481 acggcctggg gcagtgcggc attcgtcgtg cttctggcgc tgacctttgt cggcctgtcg
      541 attcttggga aactggttga aggtcgtgct gccggtgccc aaccagcggg agacacctat
      601 caagatctca tatcgcgttg ggatatgatc cgggtgcccg ccgatcagag cttgatgttg
      661 accgccttca ccgctgcggg cgcagtattg agtctcctat tctgggccgt gcttccggtg
      721 tcgactcccc atccgcacga atgttgtaaa cctgccactg tggaggctgc tccgcccttt
      781 gatgctcaaa agtccctctc taagggagag aaggcgttca aggacgcaga gctgaagatt
      841 gccgcgatcg aacagcgaac ggcagaagac gaaaaagaca agcagaaggg cggcaaggac
      901 aaagccggtg tcaaggatgg ctctcccaag agcgacggca agccttcgca atcagcagct
      961 gggaaaccgg cgggaagcgg caaatgacat catggcgaat gacgaggtga gaacaatgat
     1021 gaaccagggt cagatcgtca ccaatcacgg gagccaaatc gcaggccgtg tggccctgtt
     1081 ggccggatgt gcggcactgc tggcatgggt gggaatgccg gatgcggccc ttgctcagga
     1141 agctgccccg caggctccga ccgcgtatcg agatattccg tatatcggca gtcgaaacct
     1201 tgtctggatt atcgcccagc tccatttgct actcgccggc ttcgtgcttg gcgtgccgat
     1261 ctttgcctgg ttgtgcgaaa tcgtcggctg gaaatcaggc gataagcgtt atgacaagct
     1321 agccaaagag tttaccaagc tcttgacgtc ttcatacgcc acgacggcac tgttcggcgg
     1381 tatcctgttg ttcctgctca tcggcttcta tccgaagttg atggcttatc tcacggatat
     1441 ctttttccca tcctttctcg tctattgcgg tctcttcctt gtggaaacgg ccacgctgta
     1501 cctgtactgg tacgggtggg acgccatggc cgagggtagc gggaagaagt ttcacatttt
     1561 cctgggattt ctcctcaacg tgttcgcgtt ctttatcatg atcgtgccca actcgtgggc
     1621 gacgttccag tccagtccag tggtgatctc ggagggcagc gatttcgcgc gggcgtgggc
     1681 ggcgacgtgg aatccgacct ggtggccggt caacattcac cgcttgatcg ccaacgtggt
     1741 gctcggtggt tatatttgtg gcgcgtacgc cggcatccgg tatctgtcgg ccaagagcca
     1801 ggaagagcgc tatcactacg attggatggg atacgtcggg aactttatcg gcgtgttcgg
     1861 actcttgcct cttccattcg ccgggtactg gctgatgcgg gaaatttatc agtacaacca
     1921 gcagatgggt atcacgctca tgggcggatt cctctcctgg ttgttcattc tgcaggccat
     1981 gttgatcggt gtgctcttcc tgggctccaa ctactacttc tggctcggaa tcacccatcg
     2041 aattccgggg tcggaaacgc aatatcgcaa accgattatg gcgatgttga tcattcttct
     2101 gctctgcctc ggtgtgtgga tgaccccgca ctccctcgtt gccagcttgg aagaggccca
     2161 aaagatgggc ggcacgcacc atcctttgtt gggcgtattc ggcgtcatgt cggccaaaat
     2221 gaccgtttcc aatctcatgg tgctcgtcac attcatgagc ttcattatgt attggcgtgc
     2281 cggtaagcaa gacaccgcag gatgggccaa ggccgccaag gctctgatgg gaggtgtgct
     2341 cggcttggca gccatagcag tcatcgtgct cggtgtctgg ggttatttcg ttcccgctat
     2401 tatccgtatc aattacttct ctacctccca ggtgggtatc gtcatctttg tgatgttgac
     2461 gattactccg ctcactgcgc tccttctcaa gagcgccaag accaccaccg aaatggtgtg
     2521 gggaagaatg ccaccacgcg ccggctatgc gctggtattg aacgcggtca tggtcatcct
     2581 gctcatgacc ctcatgggct atgcgcgttc ttcatcccgc gttcactggc acatctacgg
     2641 tgtgatgcgc gattcctccc agtacgcgtt ttctcccgcg cttggttatg cggccgcgct
     2701 catggcgctg aacacgttct tgttctgtat gttggtggct tttattttct gggttgccac
     2761 gatgggagac aaggccaagg ccgctgccag taccaaaggt tcggtcgagc caggagccat
     2821 acccgcgatg gccggcgggt cgccggcgct ggatgggcct gaggccgatg cttctgagga
     2881 aaaacgtccg gtctgatcgg gaagatgatc ttcccctggt taccaggggt gggtcgtgat
     2941 cattatcctt tgttgaggac ttatgagtga agtagtcaaa ctgcagttaa tcggtctctg
     3001 tgtcgtcgga ctcggaacag tgatcctcct gttcatcaag gggcaattca ttcgggtcat
     3061 tggtttcgtg gcgatcgtgc tgggactgtt ttcattggtc gctctggccg ttccccagat
     3121 ggcctcattg ccgcctgctg aagaaagcat caacatcgcc gatatcaaga cgccgacgga
     3181 catggcgacg atcgggcaga agatcttctt cagcaagggc cagtgtgcgc tctgccattc
     3241 tatcggcccg agcgaatcgg cacgctgtcc ggatctgaag ggaatcgggg ccaagctgtc
     3301 ccgcgagttt atttttgaga gtctgaccga gccgcaagcg tatctttatc tggactaccg
     3361 acacgaggga gttccgaagg agtatccggc ccggatgccg tatatcaaca agaatccaat
     3421 cggcctgtcc aagaacgaaa ttctgtcggt cattgcattc ctgcaacaga tgagcggtga
     3481 gccgatcagc gtgagtccga ctgaattgga tttgccgcgg gcgaccccgg ctcccgtcaa
     3541 ggcggcggat gccggaacgc tcgcggtggc acaggctcga tagtcgtcgg tgaagtagag
     3601 gagggtggtt atgggaggcg tactcgttcg accgattatt ctgacggtct gtatttatct
     3661 gttcttaaag tttattgtcc cgaatctgcc gcattccgcg ccgctgccct caagcttgat
     3721 tttcctgtac ctgatgctga cgacggccgg gatcgtcatt tttgctacgc tcagcggggc
     3781 atcaaaagac gcattttttg gaccgatcct gcgctttctc actggagaag gtggcggggc
     3841 cctgagtgga gcacggtatc tcgtgttggc cttatttccc ttgctcgtcg gatggcaggc
     3901 ctacgggagc acggcctcca gtgatgcacc accgggtgaa aatcgcacga ttcatcccgc
     3961 tcctcccggt gagtataccg gtctttccaa tcccgttccg aagacgctgg acaatatcaa
     4021 ttatggcaag gggctctacg cctcgcgttg ctcgccatgc cacggcaact tcgatggaaa
     4081 gggtttcgct gcggcgggat tcacgccgcc gccggctaac tttaaggatc cgacgacgat
     4141 cgccatgctg caggaaagct atctcttctg gcggatcaag aagggaggcg taggccttcc
     4201 tgtcgaaggg atgccatgga agacggctat gccgcgatgg gaactggaaa tgtccgatga
     4261 ggatatctgg aaagttatta tgggcgaata tgacggggcc ggacagaaac caagaacgtg
     4321 ggatgagtcc gagtaattcc tccgatcaga gattgctgag caattaaggg gagcgtatgg
     4381 cacgagtgag gagcgtagga aagagatggt gggttggagc cgtcggcctt ctggggctgt
     4441 gcgtggccgc cgaggtgcag ccggcctata gccagcagag cgtgtcggtg cgcgccgcgc
     4501 tcgtgaaagg atcccttccg gcagatgatc cgacggcagc ggcctgggcc acggcggcgg
     4561 tatcggaatt cccaatgtct cctcaggtgc attggccggt tcgtattacc gacgtcacgg
     4621 ccaagagcgt caaggttcgc ggacttcatg acgggaccac ggtggcgatt cttctggagt
     4681 attcggatcc gtctgaagac ccggaggacg cagcagcctt ggagttcatg gtcggcgata
     4741 ccaaggccca tttcgcccac ggccagccca tggctcaggt ggagggcggc ccggtcaata
     4801 tctggtattg gaagaacaag gacggcaagg gcgcagacct tggtgcaaaa gggttcggaa
     4861 cgctgaagcc tcatgcccac caggatgtca aagccaaagg tgtctaccag ggtggcgtct
     4921 ggaaagtggt tttctcgcgt ccgttaagca cggagcatgt ggccgaagat acccagttca
     4981 agccagggac attcgcgagc atcgctttcg ccgtctggga cggcaagaag atggagaccg
     5041 gccagccgaa ggaaaagggc tctgaaaaag ctatttcgtc ctggtggtat ttccgggccg
     5101 atgcgccgcc ggactattcg ccgtacatgt atgcattact cgcggtggcc ttagcgctgg
     5161 gctttgagtt ggtcctcgtg agacgtttaa agaaagggtc gtgagcatga gggcgtggcg
     5221 aagaatacgc gtatttggag cggtcgcagt ggtggtggga ctctcgggcc tcattggagc
     5281 cgggggttgt tcgatgtttc agaacgaaca atccgcgaag ggcaagaagc tctatgctca
     5341 ctattgcatg cattgccacg gtgaaaatgg cagacagaac gaagggttca attggtcgtc
     5401 catgacggac ccgaagccca aagacctttc caataaagac gaaatgggca cctttaagga
     5461 tgaggaaatc tttaacacca tttcccgcga tatgaaggac acagggccag gcggggaaaa
     5521 gatcggtgat gatgaatttg ccgtcccgac catgccgacc ttcaagtaca cgctgtcaga
     5581 ggaagagatc tggggcatcg tcggatatgt gcgctcgttg cacggaaaga aattagagtt
     5641 caatgtggaa ggtcgaaaga aagaccttca agcggcccag caggcagccg atcagaaatt
     5701 taaggaagcg gaacgggtgg ctcaggaagc ggagaagaaa gccagcgacg aagccgacaa
     5761 gaagggtgtt gaagtcgacg ataacgctta tgccaaagaa atgcaggcaa tggggcaggc
     5821 caaaaaggaa ctcgaccagg ccacggccgc actggcgaat tttacgacca ggcccggcaa
     5881 gggcgtaaac attgcgcgtc ccgatctgaa tatgaagccg gatgccgccg cgaaactggt
     5941 ggaagtcggc aagcagattt atgtgaccaa gtatgggtgc aatggatgcc acaagatcgg
     6001 cgacgagggt ggaaaggtcg ggcctgcgct ggatcgtgcc ggataccgtc tgaatcccac
     6061 ctgggtctac cgatggcttc gcaacccgca agccatgaag cctgatacgc gtatgcccag
     6121 cttgggattg aacgatgcgg atgcaaaggc ggtggctctc tatctgaaaa ccctccgcgc
     6181 tccgaagcca gaccagccac tcgggaaggt gggcgaataa ccggctaggg agtctgatta
     6241 aaggtgccga tccagatacc gacgaggatc gccacgccgg aatagacgtg ctgtttgaac
     6301 atggcgaaag cgatgtgcgg agtgacgggc gcttgtaatc gtcgtacctg ccagaggaag
     6361 actccggtcg gcagggccaa tattagataa tatcccatcc cgaggccgga caactgtccg
     6421 gccactacca ggcacagtac catccccaca aggaaaactc cgacggcgac cgggacagcc
     6481 tcaccgaaga gaagtgcaga cgacttcact ccgatgcggc gatcatcctc gcagtcctgc
     6541 agcgcataaa ttgtgtcgta cgcgatggcc cagcaggtcg tcgcggcaaa gagccaccac
     6601 gccggcgctt cgatcgagcc gcgcgaggcc gcccatgcca tcacggctcc ccaaccgaag
     6661 gcaatgccca gcaccccttg cggaatctga atccatcgct tactgaaggg atagatcgct
     6721 gccagaaaga gagcgatagg actcagtagc atcgtgaagg gattgagtgt gatcaccaga
     6781 gcggccgcga tcactgccag gccaagtgcg acgagcagcg cttgtctggg cgcaaggcgg
     6841 cctgcggcga gcggtcgagt gcgtgtgcgt gcgacgtgtt tgtcgaaatt gcggtcggcc
     6901 aggtcgttaa agaccacgcc gagactccgc atcacgaagg cgcccaacgt aaagacgccc
     6961 aacagccaca tcggtggacg gccctgattt gccaggacta acgaccacag gctgggcaac
     7021 agtaagagcc aggtgccgga ttgattccgc agtcggatca ggtcggccac cgcgctccag
     7081 gaccatgcgg gtgttgagcg gtgagttgaa ggactcgcag cgttcacgcg ctggaccata
     7141 gccgagcaca ggaggactgt caacgcggca tcgcgtcggt tgttatgtct ttgtgagatt
     7201 cggtataacg aaactattgt gcagtcatct atgtgcccag gaataggacc aagtgtgcgg
     7261 cggctgtcga ggtgtcctct cgcctttctg ctgttcgtgg tatgggtgtc gagcggatgc
     7321 caattcttcg gtcagcgtag cagcctggcc actcactaca atctcccact caccgtccag
     7381 cttcggatgg atcacagtat cgctgcggcg tcgctagact atcgcgatgc ctgtggccag
     7441 gccctcaccc tgcccattcg tgacgggtta gagacgcaat tgaaaaagcg catggggcag
     7501 gtattcgaac gcgtgcacct tgaaccaggc ccatcgactg gtgccgtgga tggtgtggtg
     7561 gataccgcgt taggagtccg ccaggtggat ctgttcattc ctcgtaaggc caataaatcc
     7621 tatcctgcga cggtgacgct ggggatggag ttttcgtata ccgatcacca cggcacggtc
     7681 cttgtgagca agaaattgca gagcgctgcg accggagaag tcgagacgcg ggccgacacc
     7741 tgcaatatca gcggcctcga caaagtggcg gaagaggcgg ccgccaagct tgtggaaggt
     7801 atggcgcaac agcttggcac tgccaccaaa attcgcgagc aggcacagtt cagaaagagc
     7861 ggcgacaagg gaaccatgca gtctgctccc gtgccgcctg cgacaccggc agtggctcca
     7921 ttggcggcca caccggtggt tccggtagct cccttgcaat ccgctgccgg ctccacgccg
     7981 caggccgcag ccccggattc ggctatccga tccgggatga ctcaactctc gttccggacc
     8041 atcatccgag acgataacca gaatcatgtt ctggaacaac gtgagtcgtt cagtgtggag
     8101 tttgaagtca agaacgaggg ggctgtagtt gcggagggag tcgaggttga attgagcggt
     8161 catgccgcga tcgtcggagg cctcaagtcg ccggtctcgt tggggacgct gcagccgggt
     8221 gaagttcgcc gcgtggccgt tgacggcaag gtgggggcgg tcgcagacgc cgagcaggcc
     8281 gagttgatgt gtgccctgcg agcgtcatcc aacgtcctgc tcccgtcagc aaagaagttt
     8341 tttgtcgcga tacatcctga tgccggtgcc gctgtagagg tattgtcggt agatgtggac
     8401 caactcccga gcaggaatgg aaagtcggcg caaccgaaca gtgtcgggat cgcgatcggt
     8461 atcggcgcgt tccgggatcc ggccgtgccg acggtgaagt tcgccgcaca cgatgctgat
     8521 gtcatgggcg ggtacttcaa gaccgttctg ggcgttcctc cgcacaaggt gaaggtgctg
     8581 tccgacgcca aagcgcttag agatgacttg atcgaagtgt ttgagcagtg gttgcccaag
     8641 cagggcagtc cgcaggcgac ggtgtatatt tatctgtccg gccgggctgt ggtggatcaa
     8701 gagaccggga tcgtctcgtt gttgccttac gatgggacgc gagccggtgc atcccggatg
     8761 ttttccctcg cccggcttca gcgggctctg accaaagcct ccgtgaagca agccgtgttg
     8821 atgctggatc tctccctgga gccgtcgtcc ggttccgatc cgggccgggt ggtccctccg
     8881 cgatggaccc aacaggattc aagcggggag ccggatcggg tcatgctgat gatcggaaac
     8941 tccggcctac aggaggcgca agcctatcag ccgggacaac atgggttgtt cacatatttc
     9001 ctgctgaagg ggttgcgcgg agcggccgat ctcgacaaaa gcgggactgt gttgtccggg
     9061 gaactctgcg aatacgtgca tgatcaggtc gatgccgtta cgcgaggagc gacggggggt
     9121 ggccagccca cactctgtct gccgatgggt ggtcggcaac cggtgttaac cgggattcct
     9181 ctgagtaaga tccgttgagg gtggcactct accttccgga aggcggtggt gatgctgacc
     9241 ggacgaacac gatggccacg ggcccgtcga atcctcttcc tggcatgtct gtggctagcc
     9301 agcgttagtt gttcgattat cgttctggaa tgggtcacag ggtacctcta tgggcgcacg
     9361 cccttctcga atggaaagcg gatcatcgat ctgtacctgg ggcaacagcc gaccgttcgc
     9421 tctgatgtaa acggagagag cgcgtcactc gcgtcccatc cctacttgct ctacacgcat
     9481 ctgcccaatt tgacgacgga cgggtatcaa cagaccaatt cgcttggcta tcggaataga
     9541 gaattcactg tcgcaaaacc accggacacc attcgaattc tggtgctggg gggatcgacg
     9601 accttcatgt ggccgttcgt gaagaacccg gcggatacgt gggtggcccg cctcgaggct
     9661 aaactcaaga cgatttcgaa gaagacggtt caggtagtca atgcgggact gaactacgga
     9721 accagcgccg aggcgctcgc cggctatgtg tttcgacatc gttttctcca gcccgatatc
     9781 gtgatctatc atggcggcgg caacgacgta ctgccgcttt ttttcgggaa ttacactccc
     9841 gaatataccc acttcagacg gtatggggcg ggtgagattc cacgcccagg agagaaattt
     9901 ctgctgcagc acagcagcac agcaaaatac gtctactcca ggtggctgga gccgataggt
     9961 gcagtcgtgg taacggagcc gttttgggaa gtggatccga aaacggcgct tgaacgggta
    10021 cgaatgcagg cgcccgaagg gttccgacgg aatctcgaca tgctcgtgtc ttcgagtcaa
    10081 caggccggct cagccgtggt gttgttcggt ttcttgcaag cgagaaggca gaatctcagc
    10141 aagaatgcag gagccttcaa agggttcgaa gaggctctga ttctggggct ggaaaagaac
    10201 tatgacgtca tgcgggaaat cgcgacccgg tatcgggtgc cctttgttat tccgccccaa
    10261 gatcggtttg aagatgaatg gtttcaggac aattgtcacc tctctccagc gggcgaggag
    10321 gtcaaggcgc agatcatgtt cgaacaatta cgggatcatc ccatttttct atctgctctg
    10381 taacgggagg cgtttcaatc attgacagtg cttccatccc ctgagtaaga ttgcgctcct
    10441 tgaccgatcc gaagatgatg ttcaggtcaa caagccggcg tagctcagtt ggcagagcag
    10501 cggtttcgta aaccgcaggt cg
//
LOCUS       DAOQRM010000085        10522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_5588_length_10522_cov_13.856215, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000085 DAOQRM010000000
VERSION     DAOQRM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5588_length_10522_cov_13.856215"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PKK81_08190"
     CDS             complement(<1..911)
                     /locus_tag="PKK81_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNV32894.1"
                     /translation="MKIRAILGALVLASLPAMPALAENMTPEDIKKLVDEAVEKRLQE
                     HERREGGMQQAQGEAASTPQYPVASGPITEIRVERKGEEKIPLGFGSTGSGKLIYAKP
                     FLSAPKATVGGYADVMYNSLSRQNLDNPSRNSFGQQRLVPFIYADITDHIKFATEIEI
                     ERGGSNAPQGTDGSMQIEFAQLDYLINEAINLRAGILLMPVGKFNLLHDSPLNDLVDR
                     PMVSRIVIPSTWFEAGAGIYGTLYPSSRSKLDYEVYAVNGMSQTAGGITDLGVRSARG
                     SVSRDRDDNKAVVGRIAFSPMLGIEIAG"
     gene            1563..2588
                     /locus_tag="PKK81_08195"
     CDS             1563..2588
                     /locus_tag="PKK81_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNV32895.1"
                     /translation="MGYRFAVMSGIFLLLFSSGCATTRSESAPVVVKRTQMQMGTLVT
                     ITSVASDRRRAQDAASAGFQEIHRLEELLSTWIATSELSQVNAAAGRDAVMLSRDTMK
                     VLEASLEMARLTEGGFNILVGPAVEAWSVLDRQHIPSDAVLQAVRPLTELRSLHLNVA
                     ERTVYLAKPGMRVDVGGIGKGFAADMAVAAMQAAGATAGVVALSGDIRTFGRLPGGMK
                     FPFGVRHPRHEEAVLAFIELQDEAISTAGDYERYFERDGVRYHHILDPITLQPARDCQ
                     SVTVVARDGLTADGLDTGIFVMGRERGLALVERLPGVGAVIVDRDGKVWVSSYLKGRV
                     RMNDGIE"
     gene            2647..2943
                     /locus_tag="PKK81_08200"
     CDS             2647..2943
                     /locus_tag="PKK81_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32896.1"
                     /translation="MRAGIVVGLLVLSACSGSSKYFHDAVNEATQEMVGKRYGSPHKT
                     QAATGGGEIWTYFERGSGTAGFGGQVRGGGCRAYVLTFDKQAVLRGWEQQPCQG"
     gene            complement(2953..3345)
                     /locus_tag="PKK81_08205"
     CDS             complement(2953..3345)
                     /locus_tag="PKK81_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNV32897.1"
                     /translation="MSGPTSQPPSELATSPAQTASLVPSNSTTQALRKPDYGWLAGPL
                     LQRIEALKQYPATARLHRLEGRVIVRIVIQQDGHITSATIARSSGHDVLDQAALETIR
                     QASPLTLSQPLEKSSVTMQIPLGYYLDR"
     gene            complement(3912..4286)
                     /locus_tag="PKK81_08210"
     CDS             complement(3912..4286)
                     /locus_tag="PKK81_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNV32898.1"
                     /translation="MEREVNQINVIPLVDVMLVLLVIVLTTATFISTGQIPVNLAKAK
                     EAGDHKDVPVVVTLTANGDLFLNDRPVPPDGLKTVMLAHPRESLVVVRADKVTLLERF
                     VSVVDEIRGLGFQSVSLEVVRL"
     gene            complement(4270..4701)
                     /gene="exbB"
                     /locus_tag="PKK81_08215"
     CDS             complement(4270..4701)
                     /gene="exbB"
                     /locus_tag="PKK81_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HNV32899.1"
                     /translation="MDLLKNAVEYGIIGMLIALSIWSVAVAVERWLYYRRVDLSQFTD
                     IQTFEMALTKRLVIIGTVAANAPYIGLLGTVLGIMMTFHTMGTSGTMAVNTIMIGLSL
                     ALKATAVGLLVAIPCVVMNNILRRRVTELLTIYKVQHGTRG"
     gene            complement(4788..5909)
                     /locus_tag="PKK81_08220"
     CDS             complement(4788..5909)
                     /locus_tag="PKK81_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV32900.1"
                     /translation="MPDAMDLAAEQSPVLELRGISCAYEPNRPAVEQITLTVQQGEIL
                     CLLGPSGCGKTTILRAIAGFERVTDGSISLSGRLVSSRDVMVPTEQRHIGMVFQEYAL
                     FPHLRVEKNIAFGLSHLSRPQQKAIVDDLLTLTGLRGLEQRYPHELSGGQQQRVALAR
                     ALALRPVLLLLDEPFSNLDPDMASRMRQDLHALLRQTKTTAILVTHDHDEAFSMADRV
                     AVLNRGRLEQFDTPEAIYHVPTTPFVADFVGQADFISGVVANDLVTTEIGEFPNTQHL
                     ATGTSVVVMIRPDDIHIVPTKGADAHILARQFKGSENVYTIQLPSGQIVHSSESSLSI
                     YQVGTAIALRVVATHTVLFPQPPESSRKKEPGHGNAPPP"
     gene            complement(5963..7567)
                     /locus_tag="PKK81_08225"
     CDS             complement(5963..7567)
                     /locus_tag="PKK81_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNV32901.1"
                     /translation="MLTATRAHAPSSLQWISLITAAFLVLPTCYIIYVALTAAPTVWS
                     RLWSTRIPELLWNTLSLATGVALTTLLLGVSLAWITVRYEFPGRRVWEWALALPLAMP
                     TYVLAYVYAHLLGMGGPVEQGWQTLMGSDARLLSPQSFVGVTLIMALDTFPFVYLLVR
                     GALLNLNISFEEVARVCGVSPWSTLWRVTLPLIRPAIAAGLALVILYVISDFGAVSLL
                     RYQTLTYAVYQQMTSRYDHTSASILSLLLVVMAIIFLVTERWFRQRSRFYQTSGRYRR
                     ATRHHAGPAGTALITGYVVLVFGAAFAIPAVMLIQWSIEAIAQGALDARFFGFIWNSS
                     FLSALAACGAVIIGLPLAYLASRRPSRLNLACLQAAYAGYALPGPVAALAVLVLFTHF
                     VPVLYGTVAVLLVAYILHFLPVGLQSMEPALQQITPNVEEVARTLGCTTRRTLRRVTL
                     PLIRNGFIAAWVLMFLQTMKELPATLLLRPVGFDTLAIRVWLEASEEYYQLAAPAALL
                     IVLLSLPALLLLVSKDWRGRDQEVVS"
     gene            complement(7586..8617)
                     /locus_tag="PKK81_08230"
     CDS             complement(7586..8617)
                     /locus_tag="PKK81_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV32902.1"
                     /translation="MASSCRTFLALTLLLGALTFLQWATGTAEAAEKLVVYSGRAERL
                     IKPVLDEFQAKSGIQIELLSSGTTELVNRLQAEGDHTPADVFLTNDAGSLEHARELKL
                     LRPMNMREVERAIPSQFRAADNSWIGLSGRFWIVVYNTNLVKPDQIKSLFDLAQPQWK
                     DKIAVPNSGSEYLQAGVSVIKATFGDERTKQFLQGLKANAGTQVYQKSSQIVDAVAKG
                     QVAAGIVNHYYIYRHLATQPTAPIAAVMTDQQEGGMGAIMNVTGIGVTRASKHVESAK
                     LLIEFLVAQAGQKMFADLDKEYPLHPDVKADPTLIDRRTFRAAQVPLARLAELREATL
                     TLIEQVGLR"
     gene            complement(8713..9144)
                     /locus_tag="PKK81_08235"
     CDS             complement(8713..9144)
                     /locus_tag="PKK81_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNV32903.1"
                     /translation="MSKTLKEMDVLRQHLAKHQLKLTRQRELILTAFLRQEHVTAETM
                     YHQLAKKDPHLGLATIYRTLNLFCEAGIAQARHFGTQTQYDNISHKGHHDHLICTGCG
                     TIVEFENCEIERLQEEVATKNGFVIQTHRLELYGLCARCRH"
     gene            9298..9861
                     /locus_tag="PKK81_08240"
     CDS             9298..9861
                     /locus_tag="PKK81_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32904.1"
                     /translation="MRKSHEMTIDRVLLLYALSLAEPYGLLSDVKLQQLCFLCELQMF
                     GKGMKGFHFEFVRFAYGAFSRDLDNDLTSLRRKERVENFSPSDQVRDEVLPLLMKGID
                     GNETNEKIRDLMQAVITTYGPQDVTAITQSVESVELSTPQQPDFKIPIRDIVFHTTLL
                     VPARIEASAEFLLPPSQLAKLNTAMGY"
     gene            complement(9949..10335)
                     /locus_tag="PKK81_08245"
     CDS             complement(9949..10335)
                     /locus_tag="PKK81_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32905.1"
                     /translation="MANILIINADANLKRLIKLALPGMEHSVQHSATLQTACAQSTSS
                     KLDLVISRFVADDDQGPKDLMELRKTFPTARLIVTAALHDPALEGEKFQRVVRELHIE
                     YCLLEPIETGAMLHTIQAALALPKRP"
BASE COUNT         2120 a         2730 c         3412 g         2260 t
ORIGIN      
        1 ccggcgattt caatgcccag catcgggctg aaggcgatgc ggccgacgac ggctttgttg
       61 tcgtcgcgat cccgagagac gctgcctcgc gcgcttcgca ccccaaggtc ggtaatgccg
      121 ccggccgtct ggctcattcc attgacggca tagacttcgt agtccagctt cgagcgtgac
      181 gagggataca gggttccgta aataccggca ccggcttcga accaggtgct ggggatgacg
      241 atgcgagaga ccatcggccg atcgacgaga tcgttcaagg gtgagtcgtg cagtaagtta
      301 aacttgccga ccggcatcaa caagataccc gctcggagat tgatcgcttc attaatcagg
      361 taatccaatt gggcaaactc gatctgcatg gaaccgtcag tgccctgtgg cgcgttggag
      421 ccgccgcgct cgatctcgat ttccgttgca aacttgatat ggtcggtaat gtccgcataa
      481 ataaatggca ccagccgttg ctggccgaat gaattacgac tagggttgtc caggttctga
      541 cgggacagtg agttgtacat gacatcggca tatccaccga ccgttgcttt cggagcactc
      601 aagaagggct tggcgtaaat gagtttgccg gatccggttg atccgaatcc caacggaatc
      661 ttttcctcgc cctttcgctc gactcgaatt tcggttatgg gaccggatgc cacaggatat
      721 tgcggagtcg aggccgcttc accctgggct tgttgcatgc cgccttcacg tcgttcgtgt
      781 tcctgaagcc gcttttcgac tgcctcatca accagttttt taatgtcctc cggggtcatg
      841 ttttccgcca gtgccggcat tgccggtaga ctggctaaga cgagagcccc gaggattgcc
      901 ctgattttca tgggtatcct ccatccgcga gtaagggttt ggttggactc gggactcaaa
      961 ggtctctggt ctacagggaa aacaggtgta catgatcgat gccctggggt gtacctggtg
     1021 gtgaggcctc ctttcatata tcgaatcatg ctgtgacggt gccccagcct tcgattgagg
     1081 tgaatgggat gacgacgatg cgcttacagc gtttgcattt gagctcaagg ccttgcccac
     1141 ggactttggc aatgagttgt ccgcactcgc agcgcgtctc actgtcttgg caggtagggg
     1201 atgccaaact ttttcctgtg atcatagcgg gtggtcggtc tcgttgatga aattgagaac
     1261 gattattaat atcccgatca gcggaggaat gtcaagtctg gcgaccaatt aaaagttggc
     1321 cgcttttttg tgtgcgctgc gcgcctgcca caggtgattt tgtcggatag agtggggtac
     1381 gggaaaggag tgtttgcctg ccggtcggcg cgacgggcga catttgcatt ttgcaggcgt
     1441 cccgcagaag atggacgcgt gtgcgcgtaa atcagcgccg ggctgcgcca atgaagttgt
     1501 gtgatctatt gagtgttaag cgcagcctgt ttgtcatatt cgtcggaggt ggtgaggtgg
     1561 agatgggata tcgttttgca gtcatgagcg ggatcttcct gctcttgttc tcgtccggat
     1621 gtgcgactac aaggtctgaa tccgctcctg tcgtcgtgaa gcgaacgcag atgcagatgg
     1681 gtacgctcgt gaccattacg tcggtggcgt ctgatcgtcg gcgggcgcag gatgcggcat
     1741 cggctggatt tcaggagatt catcgcttgg aagagttgct cagtacctgg attgcgacca
     1801 gcgaactctc gcaggtgaat gctgccgctg gcagggacgc cgtcatgctc agccgcgaca
     1861 cgatgaaggt gctggaggcc tcgctcgaaa tggctcggct gacggaagga gggtttaata
     1921 ttctcgtcgg cccggcggtt gaggcctgga gtgttctcga ccggcagcac attccatccg
     1981 atgcggtctt gcaggcggtt cgcccgctga cggagttgcg ttcgctacat ctgaatgtcg
     2041 cagagaggac ggtctatttg gccaagccgg gaatgcgggt agatgtgggc gggatcggca
     2101 aaggctttgc cgcggatatg gctgtcgcag ccatgcaggc ggccggagcc actgcagggg
     2161 tcgtggccct ctcgggggat atcaggacct tcggcagact gccgggcggc atgaaatttc
     2221 ccttcggcgt ccggcatccg aggcatgaag aggctgtgct ggcttttatt gagcttcagg
     2281 atgaggcgat ttcgacggcc ggtgactatg agcggtactt cgagcgggat ggagtgcgct
     2341 atcaccacat tctcgatccg atcactttgc aaccggctcg tgactgccaa agcgtgaccg
     2401 tggtcgcccg cgacggactg accgcagatg gtctggatac cggcatcttt gtgatgggac
     2461 gtgagcgagg actggccttg gttgagcggt tgccgggggt cggtgcggtg atcgtagatc
     2521 gcgacggcaa agtctgggtt tcttcgtatc tcaaggggag ggtgaggatg aatgatggca
     2581 tcgagtaatt ccatccaagg cggacgttcc ggatggggct tggcccctgg tcggcgctgg
     2641 gaaggtatgc gcgcagggat agtagtcggc ctgctcgtat tgagcgcatg cagcggtagt
     2701 tccaaatatt ttcacgatgc ggtcaacgag gcgacgcaag agatggtcgg gaagcgatat
     2761 ggatcaccgc ataagacaca agcggctacc ggcgggggag aaatctggac ctatttcgaa
     2821 cgcggcagcg gaacggcagg cttcggtggt caagtgcggg gaggcggctg ccgggcctat
     2881 gtcttgacgt tcgacaagca ggcggtcttg cgaggctggg aacagcaacc ctgccaggga
     2941 taactctgca gcttatcgat ccaggtagta gccgagcggt atttgcatcg tgaccgagga
     3001 tttttcgagc ggctgggaca aggtgagcgg tgaggcctgc cgaatggttt ccagtgcggc
     3061 ttgatcgagc acatcgtgcc ctgagcttct tgcgatggtg gccgatgtaa tgtgtccgtc
     3121 ttgctgaatc acgatccgta caatcacccg accttccagg cgatgcagcc tcgccgtggc
     3181 gggatattgt ttgagtgctt caatccgctg gagcaaaggc cctgcgagcc agccataatc
     3241 cggttttcgt aaagcctgag tcgtactgtt cgagggaacg agcgatgcgg tttgagccgg
     3301 agaggtggcg agctcagaag gcggttgtga tgtcggtcct gacatttctg gggcaagagg
     3361 agcgtctgca cgcaggggat tcgccatttg gggcagtgag cggggatcag gggtggcctc
     3421 gaccggtgcc gatgtcgtgt gtagggaagt ttgaagttca tcctttgctt cctgaagttc
     3481 gggaggagga ggcaccacag gagcgcgttc aagcagttgt gcgggagcct ggtgaggcgg
     3541 ggtgagggct gcttcgatgg tgttgcgctc gaccgttgcc gaagagcttg aggtagttgc
     3601 cggtatggtc gggagcgctg cctgagaagg tgtgggacgc agggccgccg ctggagcgtt
     3661 acgttgagcc ggtcggataa ccgctgtagc aggtggttgc gtactgggtg cgacctgggc
     3721 aggcgtcgcc ggaggttgag aaccgacgac cgtgacatcc cattgaaagg atgaagatgg
     3781 aggagccagg ccgattttgg cgacgaggag tccggccagg agaatggcgc tgccgtgcag
     3841 gcagagcgag atgatccatc cgcttgcatg gaagcggatg aaggggtccg atatgccggg
     3901 ctggcgggac gtcacagccg taccacctcc agactgacgg actgaaatcc aagacctcgt
     3961 atttcgtcca ccactgagac aaaccgttcc agtaatgtga ctttgtcggc tcgtacgacc
     4021 accagcgatt cgcgtgggtg cgcgagcatg acagtcttga ggccgtccgg tgggacgggg
     4081 cggtcgttga ggaacaggtc gccgttcgcg gttaaggtca ccacgacggg gacatcttta
     4141 tgatctccgg cctctttggc cttggcgaga ttcacaggga tctggccggt gctgatgaag
     4201 gtggcggtgg tgaggacgat gacgagcagc acgagcatca cgtcgaccag cggaatgacg
     4261 ttgatttgat taacctctcg ttccatgttg caccttatag atcgtcagca gttcggtgac
     4321 ccgccgccgg agaatattgt tcatcacgac gcaggggatg gcgacgagca ggccgacagc
     4381 cgttgccttc agggccaggc ttaagccgat cataatggta ttcacggcca tcgtgcctga
     4441 ggttcccatc gtatggaagg tcatcataat accgaggacg gtccccagca atccgatgta
     4501 aggggcattg gcggccaccg tgcctataat gaccaggcgt ttggtcagcg ccatttcaaa
     4561 tgtttgaatg tccgtgaatt gggacaggtc cacgcgtcga tagtagagcc accgctcgac
     4621 cgccactgcc accgaccaga tgctgagcgc tatcagcatg ccaataattc catattctac
     4681 cgcattcttc agcagatcca ttcgtcgatc ctccgcctct cgtgttggtt gtctaagggg
     4741 gcacccccgc cctgctctcg gcatggaggc ctggattgag tccggtttta cgggggtggg
     4801 gcgttgccgt ggcctggttc cttctttctt gacgattcgg gtggttgcgg gaagaggacg
     4861 gtatgggttg ccaccactcg aagcgcaatc gccgtgccaa cctgataaat actcagagac
     4921 gattcgctgc tgtggacgat ctgtccggag gggagctgaa tcgtgtacac attttcggag
     4981 cctttgaatt gccttgccag aatatgggca tctgcgcctt tggtcgggac gatatgaatg
     5041 tcgtcggggc gaatcatcac gaccacgctg gttccagtcg cgagatgctg cgtgttgggg
     5101 aattccccga tctcggtggt caccagatca ttggccacga ccccggagat aaaatctgcc
     5161 tgtccgacga agtcggctac aaagggagtg gtggggacgt ggtaaatcgc ttcaggggta
     5221 tcgaattgct ccagtcgacc ccggttcagg actgcgacac ggtcggccat ggaaaacgct
     5281 tcgtcgtggt catgagtgac aaggatggcc gtggtcttgg tctgtcgaag cagggcgtgg
     5341 agatcttgtc gcatacggct cgccatgtcg gggtcgagat tgctgaacgg ttcgtcgagc
     5401 agcaacaaca ccgggcgcag ggcgagggcg cgggcgagcg cgacgcgttg ctgttgtccc
     5461 cctgagagct catgcggata ccgctgttcc agtccgcgca agccggtgag ggtgaggagg
     5521 tcatcgacga tggccttctg ctgtgggcgg gagaggtggc tgagaccgaa ggcgatattt
     5581 ttctccacgc gcagatgtgg aaacagcgcg tattcttgaa agaccatgcc gatgtgccgc
     5641 tgttcagtcg gcaccatcac gtcgcgcgat gacacgagcc ggccggacag agaaatgctc
     5701 ccgtcggtta cccgctcgaa tcctgcaatc gcgcggagaa tggtggtttt tccgcacccg
     5761 gatggtccca ggagacagag aatttcgccc tgctggaccg tgagcgtgat ctgttcgaca
     5821 gcaggacgat tgggctcgta ggcgcaggag atcccgcgta gttcaaggac gggagactgt
     5881 tcggcggcga gatccatggc gtcaggcatc gtagatgcac cctgtgcccg gtgctcgcgg
     5941 atgtctgagt tgttcacggg attcaactaa cgacctcttg gtcacgtccg cgccaatcct
     6001 tggataccag tagcagcaag gccggcaggc tcaggagcac gatcaacaat gcggcgggcg
     6061 ccgccagctg ataatactcc tcgctggctt cgagccatac acgaatcgcc agtgtatcaa
     6121 atcctaccgg ccgcagcagc aatgtggctg gaagttcctt catggtctgg aggaacatca
     6181 ggacccaggc cgcaataaaa ccgttacgaa tgagcggaag ggtcacgcgc cggagggtcc
     6241 ggcgagtcgt gcagcccagg gtgcgtgcca cctcttccac attgggggtg atttgttgga
     6301 gagccggctc catcgactgc aggccgaccg ggagaaaatg caggatgtag gcaacgagca
     6361 acaccgcgac ggtaccgtac aagaccggca cgaagtgcgt aaagagcaca aggaccgcga
     6421 gggcggcgac aggaccgggc aaggcatagc cggcataggc ggcttggagg caggcgagat
     6481 tgagtcgcga aggacggcgg ctggcgaggt aggccagcgg aagtccgatg atcacggccc
     6541 cacaggctgc cagtgccgac aggaagctgc tgttccagat gaagccgaaa aaacgcgcat
     6601 ccagggcgcc ctgtgcaatc gcttcgatac tccactggat caacatgaca gccggaatcg
     6661 cgaatgcggc tccgaacacc agtacgacgt acccggtgat gagtgccgtt ccagcggggc
     6721 cggcgtggtg ccgggtcgcc cgtcgatagc ggccggatgt ttggtagaag cgactccgct
     6781 ggcgaaacca tcgttccgtg accaggaaga tgattgccat cacgacgagg agcaggctga
     6841 gaatgctggc ggacgtatgg tcgtagcggc tggtcatctg ttgatagacc gcatacgtga
     6901 gtgtctggta gcggagcaga gagaccgccc cgaagtccga gatcacatac aaaatcacga
     6961 gcgctagtcc tgcggcaatg gcggggcgga tgagcgggag cgtgacgcgc cagagtgtgc
     7021 tccaaggaga aacgccgcag acgcgtgcca cttcctcgaa ggagatattc agattcagca
     7081 gtgctcctcg gaccagcagg tacacaaacg gaaacgtatc gagggccatg atcagtgtga
     7141 cgccgacaaa gctttgaggg gagagcagtc gcgcgtcgga ccccatgagc gtctgccatc
     7201 cttgttccac cgggccgccc attcccagaa gatgggcata cacataggcc agcacatagg
     7261 tcggcatggc gaggggtaag gcgagtgccc attcccagac ccggcgacca ggaaattcgt
     7321 accgaacggt gatccaggcg agggagacgc ccagcagcag tgtggtcaaa gcgacgccgg
     7381 tggccagcga gagggtgttc cagagaagtt cgggaatgcg cgtcgaccag agacggctcc
     7441 acacggtcgg tgcggcggtg agcgccacat agatgatgta gcaggtgggg agaaccagaa
     7501 atgcggcagt gatcaggctg atccattgca gcgaagaggg ggcgtgagct cttgtggcgg
     7561 taagcatgcg cggctacaca agggcttagc gaaggccgac ctgttcgatc agcgtgaggg
     7621 tggcctcgcg taattcggcc agtcgagcca gggggacttg cgccgcacgg aacgtgcgcc
     7681 ggtcgatgag tgtcggatcg gctttcacgt ccggatggag agggtattcc ttgtccagat
     7741 ccgcgaacat tttttgcccg gcctgggcaa ccaggaattc gatcaggagt ttggcacttt
     7801 caacgtgttt ggacgcgcgg gtgaccccga ttccggtcac gttcatgatg gcgcccatgc
     7861 cgccttcctg ctgatcggtc atgaccgcgg cgatcggagc ggtgggctgc gtggcaaggt
     7921 gccgatagat atagtagtgg ttcacaatgc ctgccgcgac ctgccccttc gcgacggcgt
     7981 ccacaatctg cgaactcttt tggtatacct gcgtgcccgc attggccttg agtccttgca
     8041 gaaactgctt cgtccgttcg tctccgaagg tggctttgat tactgaaacg ccggcctgaa
     8101 gatattcgct accggaattg ggaacggcga ttttgtcttt ccattgcggc tgggcgaggt
     8161 cgaacagtga tttgatctga tcgggtttga cgagattggt gttgtagacg acgatccaga
     8221 agcggcctga gagtccgatc cagctgttgt cggccgcgcg gaattgcgac gggatggctc
     8281 gttcaacttc gcgcatattc atgggccgga ggagtttgag ttcccgggca tgctcgaggc
     8341 tcccggcatc gttcgtcagg aagacgtcgg ccggggtatg gtcgccttcg gcttgtaacc
     8401 ggttgaccaa ctcggtggtg cccgacgaca gcaattcgat ttggatgcca ctcttggcct
     8461 gaaattcatc cagcaccggc ttaatgagcc gttctgcgcg acccgaatag acgacaagtt
     8521 tctcggcggc ctccgcggtt ccggtggccc actgcaaaaa cgtcagcgct ccgaggagta
     8581 gcgtcagcgc caaaaatgtg cgacaggatg aagccatgcg gcgtgcctcc agagtgggga
     8641 cagcctgtaa tggccatcct agttgaaatt gataagccgt ctcaagatag tactggatgc
     8701 ggaagcgatt cgtcaatggc ggcagcgcgc acagaggccg tacagctcca gccggtgggt
     8761 ttggatcaca aagccgttct tggtcgcgac ctcttcctgt agccgttcaa tctcgcaatt
     8821 ctcgaactct acaatagtcc cacagccggt gcagatgaga tggtcgtgat ggcccttatg
     8881 cgagatattg tcgtattgcg tctgggtgcc gaagtggcgg gcttgggcga tgcctgcttc
     8941 gcagaagaga ttcagcgtgc ggtaaatcgt ggcgagtccg aggtggggat ctttcttcgc
     9001 cagctgatga tacatcgtct cggccgtgac gtgttcctgt cgcaggaacg ccgtcaggat
     9061 cagttcgcgt tgacgagtca gcttcaactg atgcttggcc aggtgctggc gaagcacgtc
     9121 catttctttg agagtcttgc tcatggcgcg tgaaccggca tgaaagacgc ccattaaaga
     9181 cgaaagtgcc tcggcgggtc aagaggggat ccggccggta cgatcatcaa ttgacgaacc
     9241 gaaacctgcc tgtttatagt cctgccggtt gtggacgatt cagaggagat tcaataggtg
     9301 cgcaaatctc acgaaatgac gatcgatcga gtgctactgc tgtatgcctt atcgctggcc
     9361 gagccctatg gcttgttgag tgacgtcaag ctgcagcagc tctgtttcct gtgcgaactg
     9421 cagatgtttg gaaaggggat gaaggggttt catttcgagt tcgtccggtt tgcctatggc
     9481 gccttcagcc gcgatctgga caatgacttg acgtcccttc gacgcaagga acgggtcgag
     9541 aacttcagcc cctccgacca ggttcgcgac gaggtccttc ctctgctgat gaaaggcatc
     9601 gacggaaacg aaaccaatga gaaaattcgt gacctcatgc aggcggtgat caccacctac
     9661 gggccgcagg atgtcactgc cattacgcag tccgtcgaat ccgtcgaact cagtaccccg
     9721 cagcaacccg atttcaagat tcccatccgc gatattgtgt tccacaccac gctgctggtt
     9781 cctgcccgca tcgaagcgtc ggcggagttt ctcctgcctc caagccagtt ggcgaagctc
     9841 aacacggcca tgggatactg aaccggaggc tcccggtccg gtaggtcgtt ggcgctgacg
     9901 tgttgtggca gcggttgggt ggcgggcgac cggtgaaaat gggtcgatct agggtcgttt
     9961 cggaagggcg agcgccgcct ggatcgtatg gagcatggca ccggtctcaa tcggttccag
    10021 caaacaatac tcgatgtgga gttcgcggac gacgcgctga aatttctcgc cttcgagtgc
    10081 gggatcgtgc agcgccgctg tgacgatcag gcgagcggtc gggaacgttt tgcgaagttc
    10141 cattaagtct ttcggccctt gatcgtcgtc cgcgacgaag cgactgatca ccaggtcgag
    10201 cttggacgac gtggactgag cgcaggcggt ctgtaaggta gcactgtgtt gaacagaatg
    10261 ttccatgccc ggtaacgcca gcttgataag ccgcttcaga ttcgcatcgg cattgatgat
    10321 cagaatattg gccatagtcc tccgatgaga aggtgcaggg tgtactgacc ggccgccgtg
    10381 ggagtctgtg cagaccgatc acaggtgccc gtccgcttgg agagaattgc tgtgcagggt
    10441 cggtcaactc gatcatcctt cagtgttaga ccaactgctg gtccagggcg tagcgaatca
    10501 attctgccgt ggttctcatt cc
//
LOCUS       DAOQRM010000086        10271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_5803_length_10271_cov_14.659554, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000086 DAOQRM010000000
VERSION     DAOQRM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5803_length_10271_cov_14.659554"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..1617
                     /locus_tag="PKK81_08250"
     CDS             403..1617
                     /locus_tag="PKK81_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32906.1"
                     /translation="MSAFSAAWTRRSGRAISRFRTSLQNLAAAHAPSGISNLIDHVTQ
                     QAASARPGADLWSGIAADCRRDLSGLSVHKGTLAQAVEWETLKLQTRLRSTNGYHPDS
                     VPLFRNRHVHIGTLIQLWRRLAFDTETWLAEQGHETLLDIGPWGGFNFVVDDDGYTRM
                     PFARLTLAVGSLPGTPLDDAGGPFFQHLLPCYRAELQAAGVHFPDQWQWQFPKRDQTG
                     RLAELSGTHYLPEHTYDRRTFIKVRLSRSCETAEEITLQDLLPLLERLHFTTDWDLYR
                     EQTQPVDARFDLQDFLSLNHVVEGLYQRTAKEERLLNEIKDAYRGAVRSPQVLYKYLD
                     TVIRSGWVENLYWAMAEAALGVKRYQRAVSFDREVCPHIPPRLLIPVRRHLQRYHAGL
                     SAVAPAPTEVTA"
     gene            complement(1607..2578)
                     /locus_tag="PKK81_08255"
     CDS             complement(1607..2578)
                     /locus_tag="PKK81_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNV32907.1"
                     /translation="MTYRDLMRLANGYAESKVLLVANELGLFTAIGQGGKCADSLATT
                     CGASREGMDLLLQALAGLGLVRLKAGRYWNTTFGLNYLDGRSPTAVTNLLWLLNHHWS
                     DWTGMARAIRRGRPGWAPVTKTAAFRRRFALAMHERSYVLAPLTLDRIRLPRGARRLL
                     DVGGGSGAYSIALAQRHPDVHVLLVDQAVSVARRQIKEAGLADRIAVRQGNVLTAPLD
                     SGFDLVLISNLLHDFSEAENRGLLRRAHGALRPGGKIVIVEYFLNAAGTLPAEAAVFS
                     LLMYAFTPTGRSYAWNDVEAWLRGTGFGRFRRQLVTDSIGTLEAVRL"
     gene            complement(2575..3414)
                     /locus_tag="PKK81_08260"
     CDS             complement(2575..3414)
                     /locus_tag="PKK81_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32908.1"
                     /translation="MTGGWAVACLVFALSGLPVSVDAAVTTFIAEDYAFTGPEQLDSG
                     RQTVRLINRGRDVHQVQFLALPPGKTVADVERALASRSPSLPNWLRRHGGVNSVAPGD
                     EASVSIQLEPGDYLLLCGIPDVAGRPHAMRGMVRALRVVEAAPSGEPAPRADATLRLN
                     DFAFALSGPLQAGSRTVHLVNDGPQAHEVVLIRLAEGASAKEFIARYRPGGLPNSAGR
                     EAGGLTGLDPGREGYVHLELEPGRYGLLCFLADPVTGAPHFSRGMWMDIEVKQALPAA
                     EGP"
     gene            3611..5155
                     /gene="hpnR"
                     /locus_tag="PKK81_08265"
     CDS             3611..5155
                     /gene="hpnR"
                     /locus_tag="PKK81_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HNV32909.1"
                     /translation="MNVLLVHPSPLMYTKIYLRLEPLGLELVAAAARRAGHDVTLIDL
                     QVESHASFVTLLQERRPDAIGFSCNYLANVPEIVDLARLTKQQLPHSAVFVGGHSASF
                     VAQAMLEHADGAIDCVLKGEGESAIGPLLTAIEEDRSALAEVPGTVTRYAEGPPARMI
                     TSLNEVHPARDLLRHRRKYFIGVLDPCASIEFTRGCPWDCSFCSAWTFYGRSYRAASP
                     DKIVDDLASIREPGVFIVDDVAFIQAKQGMAIGEAVSRRGIKKQYYLETRGDVLLRNK
                     EVFQLWKRLGLAYMFIGIEAMDEEGLAHHRKRTTISKNLEALEFARSLGITVAINLIA
                     DPAWDRRRFEIVRQWCLEIPEIVNISVNTPYPGTESWHTEARHVTTRDYRLYDIQHAV
                     LPTTLPLPEFYAELVKTQQILNQKHLGWNALKSTARIAAGHLLRGQTNFVKMLWKFNR
                     VYDPKLQLADHARAVAYQMPLPPAQKQDISAGLLYIHRAQGRKGRALDDSTEQFVEAT
                     RTGASA"
     gene            complement(5200..6192)
                     /locus_tag="PKK81_08270"
     CDS             complement(5200..6192)
                     /locus_tag="PKK81_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32910.1"
                     /translation="MHLDPIARRSRKVFLVCVVALLFPAVRANDGLAQEPPGSTTLQL
                     TLDIGQSALSLGEPVYATVRLVNVGTMPVEVFKLLDPQTGAIQIEVSSSNRPRFVFLP
                     LFYANAVHNRIALAPGEAVVAIFPVFYGALGWTFDRPGTYRVTAEYRPQGASQQDRIR
                     SHAVSVTVTDEHGAGASLLTGSSAGEEAAKFLLWQRGDQLQAGQALLTGLLTQHPESP
                     LADYALLAFGRNLSRSFRNYAVGKIREADCGSALTYFQRIRSDRLPVFLQIQQRLDEA
                     RCFIKLSQPAKALDSMERAEQLRDGRPEYTLLFQQAIRLEPTLGLHSAINTPHR"
     gene            complement(6209..6964)
                     /locus_tag="PKK81_08275"
     CDS             complement(6209..6964)
                     /locus_tag="PKK81_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32911.1"
                     /translation="MLASPLRVLVSMAIPVFAGLLVSCVSLEFDRMTGTAFPTSQTVN
                     GQTVTLSSIFDEAGIKLTVEQDDTGIQPLNIAQDECISDAELSTLENGHRHLSLFPTA
                     ACPFDFCNTYHLYGAVVNHYGEVLDVCIPEFILGKMWQGHTRSAFAIFYRMNTIQTDG
                     AAYFRTTAHEIGHAFNLHHSDGDGTSIMTQSDDLTGDPVYRFSEQSREHLANHPGQCK
                     FPGAIGAAPFTWVANDHAAWAHPLEELTITDCQ"
     gene            7383..7727
                     /locus_tag="PKK81_08280"
     CDS             7383..7727
                     /locus_tag="PKK81_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32912.1"
                     /translation="MRRITLLMLLLYGCVPPPPHQFVPGGPLGEAGQTVQGPRQASDE
                     EVRTMLAESSLHQQNPPERVDLNGLLTQITQATNFRQKQRLVDRYHHAALLLPEAERD
                     QAFQRLRDTTGK"
     gene            7768..8484
                     /locus_tag="PKK81_08285"
     CDS             7768..8484
                     /locus_tag="PKK81_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32913.1"
                     /translation="MKRIPLCTLLLFTTVILGGLLPTSTPAEAHDWLGDCVVDFDNTF
                     ALTYIYGQARGNFGYPTGIDNSGELQPCNATHQACWTYRHRCFGNYINVDDMTYGHIH
                     LVFDSPSLLTPCFVDPGDGPGAGFGRNVGDDCVAADWIHEPRTLGTHGKDHWIKIWME
                     DRVTHQPRVFDMPIIRVTGTEPIQFWFRKSNGSWWYWSNLAPNPWLVGGWVFDVTEVR
                     IRGAQAANGPYFIGAFSILD"
     gene            8557..8754
                     /locus_tag="PKK81_08290"
     CDS             8557..8754
                     /locus_tag="PKK81_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32914.1"
                     /translation="MALAAKVVKRTKDSFQFKISKENFESFCDSIGLYRREFLDALDT
                     SEKDHKAGRITKRKSLRELIA"
     gene            8762..9040
                     /locus_tag="PKK81_08295"
     CDS             8762..9040
                     /locus_tag="PKK81_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNV32915.1"
                     /translation="MLELSTTATFDALFSKLPKPIQRKAASKTDLFRENPFYPSLHTE
                     KLRPKHHEVWSFRVDRSYRIIFKFLGPNQVQLRYIGHHHSIYDYDMFK"
     gene            complement(9160..9456)
                     /locus_tag="PKK81_08300"
     CDS             complement(9160..9456)
                     /locus_tag="PKK81_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32916.1"
                     /translation="MALLLAFYLIFSAVIPASALEDSPAAAALVIKADVLYWEEGELV
                     VKEFSGHEERLKVTPETKIEGVVGGRLKTGDKIVAQITDDRKALSIKLQVPDGR"
     gene            9718..>10271
                     /locus_tag="PKK81_08305"
     CDS             9718..>10271
                     /locus_tag="PKK81_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32917.1"
                     /translation="MTDRVGKFNTRMADNQTISAIVTGGIQLQLIPPPKRTCSVSAIE
                     PKGVVYTKKWVVELLLDLSGYCSDKNLVDTLAIEPAAGDGAFLGPMIERLVESCGNLG
                     RSLSECEHSLIAYELDETSAARARAVAQTILMNRGAKHTLAKQLAEAWVVNRDYLLAP
                     NSTRADFIIGNPPYIRLEEIPEETA"
BASE COUNT         2221 a         3201 c         2905 g         1944 t
ORIGIN      
        1 cagcggcggc agcccgtgcg agtgcggaga cggcgctaac gccgcacttc caccgccgcc
       61 aggcacaagc tggttcagtc gaggcgccca ccgcctaccc tctcgggcat cgcttcacgc
      121 accgctcatc cccattcgct gaccgggatt cgccgcattc agagccggcg cttgtctggt
      181 gatcgcaacc ttctaccttc ccaaatcctc acgattctca gtcccctcgt aatcttcttg
      241 cccagacctt cggggtttac gagttccgcc tcatagcccg agtaccgtat gaaagggcgg
      301 gcacatgatt ccccgctgca ggaagggcct cgcacatcct ggcggagcaa cgggagctca
      361 gtccttgtga tgtgtgatgc ctgtacgagc agggcctccg acatgagcgc gttcagcgcc
      421 gcttggacgc gccgctccgg ccgggccatc agccgcttcc gaacttccct tcaaaatctg
      481 gcagcggctc atgccccgtc ggggatctcc aacctgatcg atcacgtcac gcaacaggcg
      541 gcctcggcca ggcccggagc ggacctttgg tccggaatcg ccgcggattg ccggcgcgat
      601 ctctccggac tgtccgtcca caagggaacc ttggctcaag cggtcgagtg ggaaaccctc
      661 aaactccaga ctcgccttcg cagcacgaac ggctatcatc ccgactctgt cccgctgttc
      721 cgcaatcgcc acgtacacat cggcaccttg atccaactct ggcggcgtct ggcattcgac
      781 accgaaacct ggctggcgga acagggccat gaaacccttc tggacatcgg accgtggggc
      841 ggattcaact ttgtcgtgga cgacgatggc tatacccgca tgcccttcgc cagactcact
      901 ctggcagtgg gcagtctgcc gggaacgccg ctggacgacg caggcggccc cttcttccag
      961 cacctgcttc cctgctacag ggcggaatta caggcggccg gggttcactt cccggaccaa
     1021 tggcaatggc aattcccgaa acgcgaccag acgggtcgat tggcggaact ctccggcaca
     1081 cactatttgc ccgagcacac gtacgatcgg cggaccttca tcaaggtccg cctctcccgc
     1141 tcctgcgaaa cggcagagga aatcacgttg caggacctgc tgcccctgct ggagcggctc
     1201 cacttcacga cagattggga cctctaccgt gagcagacac aacctgtcga tgcccgcttc
     1261 gatctgcaag acttcctctc gctcaaccat gtggtggaag gcctgtatca gcggacggcg
     1321 aaggaagagc ggctgttgaa cgaaatcaaa gatgcctatc gaggcgccgt acgatcgcct
     1381 caagtcctgt acaaatatct ggacaccgtg atccggtcag ggtgggttga gaatctctac
     1441 tgggcaatgg ctgaagcggc actgggagtg aagcgatatc aacgggcagt gtcattcgat
     1501 cgcgaagtct gccctcacat cccgccacga ttgctcatcc cggtccggcg gcacctccag
     1561 aggtatcatg cagggctgag tgccgtcgcc ccggcaccaa cggaagtcac agcctgacgg
     1621 cctccagcgt gccgatactg tcggtcacca actgccgacg aaaccgtccg aatccggtac
     1681 ccctcagcca ggcctcgacg tcgttccagg cataactccg cccggtcggc gtgaacgcgt
     1741 acatcagcaa cgaaaatacc gccgcctcgg cagggagggt gccggcagcg ttgagaaaat
     1801 actccacgat cacaatcttt cccccgggtc gtagcgcgcc atgcgcccgt ctcaacagac
     1861 ctcgattttc cgcctcgctg aaatcatgca gcagattgga gatcaatacc agatcgaatc
     1921 ccgagtcgag cggggcagtc aacacgttcc cctgccgcac cgcgatacgg tccgccagcc
     1981 cagcctcttt gatctgccgt cgcgcgaccg acacggcctg atcgaccagc agcacatgaa
     2041 catcgggatg acgttgcgcc agagcaatgc tgtaggcccc ggacccgccg cctacatcca
     2101 acaggcgcct agctccacgc ggcagccgga tcctgtcgag cgtgagcggc gccagcacat
     2161 aactacgctc gtgcatcgct agggcaaacc gtcgccggaa cgccgcggtc tttgtgaccg
     2221 gtgcccagcc gggacgaccc cggcggatgg cgcgcgccat ccccgtccaa tccgaccaat
     2281 ggtggttcag cagccagagc agattggtca ccgccgtcgg gcttcggcca tccagatagt
     2341 tcagcccaaa cgtcgtattc caataacgcc ccgctttcag acgcaccagt ccaagccctg
     2401 cgagcgcctg cagcaggagg tccatgccct cgcgcgaagc gccgcaggtg gtggccagtg
     2461 agtcggcgca cttgccgccc tggccgattg ccgtgaacag gccgagttca ttggcgacga
     2521 gcagcacttt cgactccgca tagccgttcg ccagccgcat caagtcccga tacgtcacgg
     2581 accctccgcg gcgggtagcg cctgcttcac ctcaatgtcc atccacatgc cgcgggagaa
     2641 gtgaggggct ccggtcaccg ggtcagcaag aaaacagagc agaccgtatc gccccggctc
     2701 cagttcgaga tgaacataac cctctcgtcc cggatcgaga ccggtgaggc cgccggcttc
     2761 ccgcccagcg gaattgggca gtcctccagg cctgtaccgc gcgatgaact ccttcgccga
     2821 cgctccctct gcgagacgaa tcagcaccac ctcatgagcc tgcgggccat cattcacgag
     2881 atgcacggtt cgagaccccg cctgcagcgg gccactcaac gcaaacgcaa aatcgttcag
     2941 acgcagcgtc gcatcggccc gcggcgccgg ttcgccggaa ggcgcggcct ccaccactcg
     3001 cagcgcacgc accatgccgc gcatggcatg gggacgaccc gcgacgtccg gaatgccgca
     3061 gagtaacaga tagtctccgg gctcaagctg aatgctgacg gacgcctcat cgccgggagc
     3121 gacactattc actcccccat gacgccgcag ccaattcggc aagcttggcg accgggacgc
     3181 caatgcccgt tcgacatcgg ccacggtttt tcccggtggc agcgccaaaa attgaacctg
     3241 gtgcacatcc cggccgcgat tgatcaatcg caccgtttgc cggccggagt cgagctgctc
     3301 cggaccggtg aaggcatagt cttccgcaat gaacgtcgtg acggcggcgt caacggaaac
     3361 gggcaggccc gacagagcga agacgagaca ggcaaccgcc cacccgccgg tcacggaccg
     3421 gaccgacatc ctattgaggc gttgagcaga catatccggg tgagaatact ccgaagccgg
     3481 gccggttcct agcccggact tctgtcctcc ccctgggcgg accacgggag aacgaggact
     3541 ctccctagtc ctcaacatgt cacagacatc ctatagtcag ccaccacacc accatcgagg
     3601 gaggtaaggt atgaacgtgc tgctcgtcca tccaagccca ttgatgtata cgaaaattta
     3661 tctgcgcctg gaaccgctgg ggcttgagct cgtcgcagcc gccgcgcgcc gggccgggca
     3721 cgacgtcacg ctcatcgatc tgcaagtgga gtcgcacgcg tcgttcgtca cgctgctgca
     3781 ggagcgtcgt cccgatgcga tcggcttttc ctgtaactat ctggccaacg tgcccgaaat
     3841 cgtcgatctc gcgaggctca cgaaacaaca actcccgcac agtgcggtct tcgtcggcgg
     3901 gcacagtgca tcgttcgtgg cccaagccat gcttgagcat gcggacggcg cgatcgactg
     3961 tgtcctcaag ggcgaaggcg agagcgccat cgggccgctg ttaacggcca tcgaagaaga
     4021 ccggtcggct ctcgcagagg tccccggcac cgtcacgcgc tatgctgaag ggccgcccgc
     4081 ccgtatgatc accagcctga acgaggttca tccggcgcgc gacctgctgc ggcaccggcg
     4141 caaatatttc atcggcgtgc tcgatccctg cgcctccatc gaattcacca gggggtgtcc
     4201 ctgggactgc tccttctgca gcgcctggac cttttacgga cggagttatc gcgctgccag
     4261 ccccgacaag atcgtggacg atctggcgtc cattcgagag cccggcgtat tcattgtcga
     4321 tgatgtcgcc tttattcaag ccaaacaggg catggcgatc ggagaggccg tgtctcgacg
     4381 aggaatcaag aagcagtatt acctggagac gagaggcgac gtgctactcc gcaacaaaga
     4441 ggtgtttcag ctgtggaagc ggctgggcct cgcctacatg ttcatcggca tcgaggcgat
     4501 ggatgaagaa gggctggcgc accatcgcaa acgcaccacg atcagcaaga acctcgaagc
     4561 gctcgaattc gcccgctcgc tcggtataac ggtcgccatc aacctgatcg ccgatcccgc
     4621 ttgggatcga cggcgcttcg agatcgtccg gcagtggtgc ctggagatcc ccgagatcgt
     4681 gaacatcagc gtgaacacgc cctatcccgg aaccgaatcg tggcataccg aagcccggca
     4741 tgtgacgact cgggattacc gcctctacga catccaacat gccgtccttc cgaccacctt
     4801 accgctgccg gaattttatg cggaattggt caagacgcag cagatcctga atcagaagca
     4861 cctgggatgg aacgccttaa aatcgacggc gcgtatcgcg gccggacatc tgctgcgcgg
     4921 ccagacaaat ttcgtgaaga tgctgtggaa gttcaatcgc gtgtacgatc cgaagcttca
     4981 actggccgat catgcccggg ccgtcgcgta ccagatgccg ctcccgcctg cacagaaaca
     5041 agacatcagc gccggcctgc tgtacatcca tcgtgcccag ggtcgcaaag gccgggcctt
     5101 ggacgattcc accgaacaat tcgtcgaagc gacgagaacc ggagcgagcg cataatggtc
     5161 gacagggaaa agaccgtcat cgaggttcac agagcagatt tagcggtgcg gtgtgttgat
     5221 ggcagagtga aggcccagcg tcggttccaa ccgtatcgct tgttgaaaga ggagcgtgta
     5281 ttcaggacga ccgtcgcgta actgttcggc ccgctccatc gaatcgagcg ccttagccgg
     5341 ctgggacaac ttgataaaac agcgggcttc atccaggcgt tgttggatct gcaagaagac
     5401 cggcagccgg tctgatcgga ttctctgaaa ataggtcaac gccgacccgc agtccgcttc
     5461 acggatcttc ccaacggcat aattgcggaa actccggctc agatttctcc cgaacgccag
     5521 cagcgcataa tcggccagcg gcgactcggg atgttgggtc agcagcccgg tcaacagcgc
     5581 ctgtcccgcc tgcaattggt cgccccgctg ccataacaga aattttgccg cttcctcgcc
     5641 cgcggacgag ccggtcagta aggaggcccc ggcgccgtgc tcgtcggtca ccgtcacgct
     5701 gaccgcgtgt gagcgtatcc gatcctgttg agacgcgccc tgaggacggt attcggccgt
     5761 cacgcgatag gtcccaggtc gatcgaacgt ccatcccagc gcgccataaa agacaggaaa
     5821 gatcgccacg actgcctcgc ccggagcgag agcgatcctg ttgtgcacgg cattcgcgta
     5881 aaacaacggc aggaacacga accgtggcct attcgagctc gacacttcaa tctggatcgc
     5941 gccggtttgc gggtcgagca gtttgaacac ctccaccggc atcgtcccca cattgacgag
     6001 gcggacggtg gcatagaccg gctctccgag actcagcgcg gattgtccaa tgtctagcgt
     6061 gagctggagc gtggtggagc cgggcggttc ctgggccaag ccgtcgtttg cccgcaccgc
     6121 cgggaacagg agcgcgacca cgcagacgag aaacaccttc ctggaccggc gcgcgatagg
     6181 atcgaggtgc ataagccgcc cttcagcctc actgacaatc ggtgatcgtt aattcttcca
     6241 gaggatgcgc ccaggccgca tggtcgttgg cgacccacgt gaatggcgca gctccgatgg
     6301 cgccggggaa tttgcactgc cccggatggt tggccagatg ctcccggctt tgctcggaaa
     6361 agcggtacac cggatcgccc gtcaggtcgt ctgattgcgt catgatcgag gtgccgtcac
     6421 cgtcactgtg gtggagattg aaggcatggc cgatctcatg ggccgtcgtg cggaagtagg
     6481 cagccccgtc ggtctggatg gtgttcatgc gatagaaaat ggcgaaggcc gacctggtat
     6541 gtccctgcca cattttgccc aggatgaatt caggaataca gacatcgagc acctcgccgt
     6601 agtgattcac caccgcaccg tagaggtgat aggtattgca gaaatcaaag ggacaggcgg
     6661 ccgtgggaaa cagcgagagg tgacgatggc cgttctccaa cgtcgagagt tcggcatcgc
     6721 tgatgcactc gtcctgggcg atgttgagcg gctgaatccc cgtgtcatcc tgctcgaccg
     6781 tcaacttgat gcctgcctcg tcgaagatcg agctcaacgt cacggtctgg ccattgaccg
     6841 tctgggaagt ggggaatgcc gttcctgtca tacggtcgaa ttccaaggac acgcaggaga
     6901 ccagcaagcc tgcgaaaacc ggaatcgcca tcgagaccag gacacgaagc ggcgaagcga
     6961 gcatgacgac ctcctgacga acagcgactg ccgtggttcg gagggcaggg gagaatggtc
     7021 tcctaggcta cgtatttcag gactgcctgt caatccgtgc cggccgctcc ggaccgaatg
     7081 cccctaccgg ctccttcaat cgacgggatc ttttcctgtt cttagttcac gtcatcgtga
     7141 acaaccctac gtagtggtat cggatgggat acggatctgt atccgatgcg atacctcctc
     7201 tgccacccac aaataccagc agttccagac accgcttcca agcgattgtt attacgcgca
     7261 gaggctctgt ctgagttcga tggcgcagag tttgcgtgat gtgaagtcgt gaccagacct
     7321 cctgcggagc gagctttctg gccacgcttc caggatcgac agcagaagac aaggagggaa
     7381 cgatgcgccg gatcactctg ctgatgctgc ttctctatgg atgtgtcccg cctcctcctc
     7441 accagttcgt gccgggaggg ccgctcggcg aagccggaca aaccgtgcaa ggaccacggc
     7501 aagcgagcga tgaagaagtg cgcaccatgt tggcggaatc gtcgctgcac cagcagaatc
     7561 ccccagagcg agtcgatttg aacggcttgc tcacacagat tacccaagcc acaaactttc
     7621 ggcagaagca gcgtttggtg gatcggtatc accatgcggc actgcttctg ccggaagccg
     7681 agcgtgatca ggcgtttcag cggttacgag acacgaccgg aaagtgaccg cgaacggagc
     7741 ccgacctgag cgagaaagga aaggaccatg aagcggatac cactctgcac actgctgctc
     7801 ttcacaaccg tcatcctggg cggactctta ccgacatcca caccggccga agcccacgat
     7861 tggctgggtg actgcgtggt cgacttcgat aatacgtttg cgctgaccta tatctacggg
     7921 caggcaagag ggaacttcgg ataccctacc ggcatcgaca attcaggcga gttgcaaccg
     7981 tgtaatgcca cccatcaggc ctgctggacc taccgccatc gctgcttcgg caattacatc
     8041 aacgtggacg acatgaccta tggccacatc cacctggtct tcgacagtcc gagtctgctc
     8101 accccctgtt ttgtcgatcc cggcgacggg cccggcgcag gctttggacg gaacgtcggc
     8161 gacgactgcg tcgccgccga ttggattcat gaaccgagaa ccctcggcac gcatgggaag
     8221 gatcactgga tcaagatctg gatggaggat cgggtgacgc atcagcccag ggtgttcgac
     8281 atgccgatca tcagagtcac cggcaccgag ccgattcaat tctggttcag gaaatcaaac
     8341 ggcagctggt ggtactggtc gaacctggcc cccaatccct ggttggtcgg cggatgggtg
     8401 ttcgatgtga ccgaagtgcg gatcagaggc gcgcaggcgg cgaacgggcc gtatttcatc
     8461 ggcgccttct caatcctcga ttaagcgaga gccacggtgt ttcagattgt ctctcgcctc
     8521 tgaaggcggt aaaattagcg cataacgagg gacatcatgg cgcttgcagc aaaagtggtg
     8581 aaacgaacca aagacagttt tcagttcaaa atctcaaaag aaaattttga gtctttttgt
     8641 gattcgatcg gcctctatcg ccgagaattc ctcgatgcgc tggacacctc tgaaaaggac
     8701 cacaaagcgg ggcgaatcac gaaacgaaag tcgctacgag agttgatcgc gtagttcgat
     8761 catgctcgag ctttccacta ccgcaacctt cgacgccctt ttctccaagc ttcccaaacc
     8821 aattcaacga aaggccgctt ccaaaacgga tctcttccgg gagaatccgt tttacccatc
     8881 gctccataca gagaagctcc gccccaaaca tcacgaagtg tggagctttc gtgtcgatcg
     8941 atcctatcgc atcatcttta aattcctcgg cccgaaccag gtgcagctcc ggtacatcgg
     9001 ccatcaccac tcgatttacg actacgacat gttcaagtaa cggtaagagc ggatgaagct
     9061 ccgaattgtg acggtgcaaa gccgaaatgg ttgcctgaat atagatgcag gtgcggctca
     9121 cggtcggcac tgagtgagcg tgagcctggc cagaggcccc taccggccgt ccggaacctg
     9181 caacttgatc gagagcgcct tacggtcatc ggtgatctgc gccacgatct tgtcgccggt
     9241 cttcaagcga ccgcccacca cgccctcgat tttggtctca ggcgtcactt tcaggcgttc
     9301 ttcgtgaccg gagaattcct tcacgaccag ttcgccctct tcccaataca agacatcggc
     9361 cttgatgacc aaggcggcgg cggccggaga atcctctaac gctgatgcgg gaatgactgc
     9421 ggaaaagatg aggtagaacg cgagaagaag tgccatcaca aaccctcccg ttttttttga
     9481 gagtaccggt gattgcggag aactgtcaac acggaactat ggctgccgat gaagattacc
     9541 ccaatgagaa actcattctc tgggccacag tccaccacca ctggcccgca gccaatgcac
     9601 tcaaaaagtg ttcccatctc ccttcttttc cagcgccgca cgtgagtaga tagaccgctg
     9661 ttggtgagtg aatcttgaac agatgcagca tacaagttag tatcaaccgc ttgcttcatg
     9721 acggaccgcg tcggaaagtt taatacccgc atggcggaca accaaaccat ctcagcaatc
     9781 gtcacaggtg gcatccagct ccagctgatc ccacctccca agcgtacttg ctctgtttca
     9841 gcaatcgagc caaagggcgt cgtatacact aagaaatggg ttgttgaact acttcttgat
     9901 ctgagcggtt actgctcgga taaaaatctt gtggatacac tagccatcga accggcagct
     9961 ggggacggcg catttcttgg ccccatgatc gaacggttgg ttgagtcgtg cggcaatctc
    10021 ggacggtcct tgtcagaatg tgagcattct ctcatcgcat atgaattgga tgaaactagt
    10081 gcagctcggg cgcgtgctgt cgcccaaacc atcctgatga atcgcggtgc aaaacacact
    10141 ctagccaagc aactggccga agcatgggta gtaaaccgag actatcttct agcacctaat
    10201 agcacacggg ctgattttat tatcgggaac cctccctata tacgtcttga agaaatcccg
    10261 gaagaaacgg c
//
LOCUS       DAOQRM010000087        10050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6007_length_10050_cov_19.519060, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000087 DAOQRM010000000
VERSION     DAOQRM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6007_length_10050_cov_19.519060"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PKK81_08310"
     CDS             <1..866
                     /locus_tag="PKK81_08310"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HNV32918.1"
                     /translation="QVRQRESALRKALKKLLRRMEGLRRDLEQAGRYEPYARYGELLK
                     ANLGLLKKGMTAVSVVDYYDERLPELSIPLDPTKGPQANMDAYFAKYRKFVSAQREIA
                     PRVAAIDTDVQQLQAELETIKNGTWRAPSGEHRTTTGTPSRQVRKRGADEERRGPFRR
                     FISSDGHPIFVGRNARENDELTFGLAKSEDLWLHARGTPGSHVVVRLEKGTDPPPETI
                     RDAATLALLYSDLKKSGKGDVMYTRRKWVKKAKGQAPGAVTVTQEKTIYITLDKTRLA
                     ALKARNTEGGP"
     gene            935..2977
                     /locus_tag="PKK81_08315"
     CDS             935..2977
                     /locus_tag="PKK81_08315"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV32919.1"
                     /translation="MGTGTDRRDRFRILNALLQAKTLRQFDERLQQELAPLLGCEIIG
                     LYLYSESTAAFSPVSESLRTPGSPAYPIDQLPAAGTIKEAAVLAGQAIFTHDLSASPW
                     AEAQAIDPTTCQATSVLVSPIRIPAESQPCATAKTLAVMVAVSPGRPGLFSEEDRAFL
                     DMLGLHIAPVLTAVLAAEERDTLVAINSQVVLGTMTLDNLLSSIQPILREVIPHDTTG
                     LVRFIGAPHGPWFEILSCEGVAVDQEALKQFPFERMAPAEILATGKPLLLTGYNQERF
                     AEHTYFESLGVFSAMLCPLLVRGAPYGFLAIGSRRRNAFSERDLALAEQIGFHLSHAI
                     ANLSAYDQIRQLKDQLEQENVYLREEMGASLDLTSLIGNSAALQKSLKAIEQVAPTDS
                     TVLITGETGTGKELVAQAIHRLSPRQHKALITVNCAALPPTLIESELFGHERGAFTNA
                     TSRKLGRFELAHGGTIFLDEVGELPIDLQMKLLRVLEAQEFDRVGGSHTIRVDVRVLA
                     ATNVDLDQAIKRGSFRADLFYRLNVFPLRLPTLRERREDIPLLARHFVKKYSQRHRKP
                     VTRINSAALNALSGYDWPGNVRELEHVIERAVIVSQGSAITVDELDGLGRGEAPSPEP
                     RTLAEAERAHIVETLSRTNWILAGKQGAAEKLGLKRSTLQHRMKKLGISRPPRQAS"
     gene            3154..3570
                     /locus_tag="PKK81_08320"
     CDS             3154..3570
                     /locus_tag="PKK81_08320"
                     /inference="COORDINATES: protein motif:HMM:NF039959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6022 family protein"
                     /protein_id="HNV32920.1"
                     /translation="MKNVGSRLTPDTVSHEIEAYVAARQAELLPSGEASKDGRRFSWC
                     GEILDFLTEGLAERLAAKGITFTDAFPGPLRLMDEDQQPNGQHVRRFWTVALHHGCPI
                     ARICTYFFHRHDQVSLPQLPRVVAYAPTGPAQEEHE"
     gene            3567..>4273
                     /locus_tag="PKK81_08325"
     CDS             3567..>4273
                     /locus_tag="PKK81_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV32921.1"
                     /translation="MNYVALRMLFGDRAKYLMLLCGLTFAVMLIVQQGSIFWGLMIWS
                     QSSISNLNVPIWVTDPGIAQVDEVKPIADTTVDRVRSIPGVEWAVPLYKGLLRARLAN
                     GEYHQITLTGLDAATLIGRPAEVLEGRFEDLRQPDAVALDQWAVERMGGPTVITVGTV
                     FELNDKLARVVAIAKTQKTFTNIPVVYTTYERALRYVPRERRTLSYVLAKAKDSAPVD
                     EVIQRIRDHTGLGAFTA"
     assembly_gap    4274..4373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4529..5287
                     /locus_tag="PKK81_08330"
     CDS             4529..5287
                     /locus_tag="PKK81_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV32922.1"
                     /translation="MDGSSQSQGRTTEGNGAEQTIAVQVRGLVKSFGSGETTVTVLKG
                     IDLDVYFGELLLLVGESGGGKTTLLSAIAGILDIDAGELDVLGASLTNMSPGTRTRFR
                     GQTMGFIYQQFNLLPALTAAENVAIPLLIKGTRKKEAISRGRLMLERVGLGDRTEFLP
                     RNLSGGQQQRVAIARALVNEPQLLVCDEPTAALDGPNGQKIMELIRDVGRAPDRCVIV
                     VTHDSRIFQFGDRMAELTDGRIVGIHPIQREATA"
     gene            5284..6276
                     /locus_tag="PKK81_08335"
     CDS             5284..6276
                     /locus_tag="PKK81_08335"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV32923.1"
                     /translation="MTILNRFSVWLAVAGAMLAAWVVLTAGKNQPMPTPIVEPPRSPY
                     ETTVAATGIIEAANENVRIGPPMAGLVTKVFVAVGDRVREGDPLLQLDDRDLRAQLVA
                     RQAAIPPAEAQVEEQKYRIGDLDTQLKRLKSVRDSRAVSEDDVKRTWYASEMAKRALS
                     RYEATLKQVIAQRDETQMLLDRLTVRAPRAATILQVNVRAGEFALTTGATEPLMLLGD
                     MQQLQVRAEVDEVNAPLVAPNRPGIAYLKGNTAQAIPLTFVRIEPYIVPKKSLTGDNS
                     ERVDTRVLQIIYRFERPAFPIYAGQQVDVFIERAPDGPPAPAPGSARSAEEPSK"
     gene            6273..7793
                     /locus_tag="PKK81_08340"
     CDS             6273..7793
                     /locus_tag="PKK81_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNV32924.1"
                     /translation="MKRVTSRRQLAISTVLVMLSLSASGCLQGSNYHRPPVETPADWT
                     RMASGPLAAGATDTMPEADWWQAFHNQELTQFVERALTQNHDVRRAVSRVLEGRASVT
                     TAGAGLYPQLNVQGSYSNIVVSKNTLAGLGLATGQQPGPQVFAKPGSSFDLWNGAADL
                     RWELDVWGRIRRGMEAASADAQAIEQDARAIALTLIGDVGQSYFRIRELDEQIEIAQR
                     ALTLRRDSLDIITKRASVGLASDLDVKRTEVLVAESAGQIPDLTRLRAVELHRLEVLT
                     GSAPGSVVLAPTSLRKVIVQPEIPVGLPSQLLERRPDILQAEASLVAANARIGQARAY
                     FFPTLSITGQGGLQSAEFANWFSGNSANFSIGPSVTLPIFLGGTNVARLEAAESRYQQ
                     MLEGYQQTILLAFREVADLLVSIHSRTEQLARQREQATAATAAVRLAEVRYRKGLVNY
                     LDVLDAQRTMLAAETQLAQTERARLTDMVGLYKALGGGWQTPGNNLSAIPAGSATH"
     gene            8023..8919
                     /locus_tag="PKK81_08345"
     CDS             8023..8919
                     /locus_tag="PKK81_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV32925.1"
                     /translation="MQDGVRGHSPARRVSPALILLVLGLTTFFPTYTSAAPLDKPVSK
                     PGMDDKPGLAEEQILSTTDKITQPVDTDAEAMRHNDLGVAFVFKGDLGQAIDEFKHAL
                     RLQPNYFAAHLNLANTLLDVGRNDAAMVEFKEALRLKPDDPKTHNDLGVALKEMGNLE
                     GAIAEFRTVLRHNPSDVNAHNNLGVTLKAMGDLDGAIAEYRTAASLQPNDVNAHFNLG
                     LGLMEKRQPEAAVSEFRTALHLRPNDAKIRLNLGNALAGMGQRMEAAQELRQYLRLEL
                     DTPANRRWLEQAEAKLRELEMP"
     gene            9112..9816
                     /locus_tag="PKK81_08350"
     CDS             9112..9816
                     /locus_tag="PKK81_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32926.1"
                     /translation="MRGWILAVLCGIPLWPGQSLPAEQEDPETSHTRQWDFDSIAPGT
                     LPSSFVIGTLFDGRPAGEWKILITDRAKSPSQVLAQLQPKGTDQAHKLLLMEGTDSGN
                     IDVEVSYLAVAGKADFGGGLVWHATDDRNYYLLRASSVEQKVRLYRVVKGVQQIVKQL
                     DRPLPANGWHKLRIVQRGCELKALYDDAVLFRVCDQTFSNGRIGLWTKADAVTYFDDL
                     VLRLLDDPLSNGRPPR"
BASE COUNT         2109 a         3197 c         2833 g         1811 t
ORIGIN      
        1 cccaggtccg acagcgcgaa tcggcactac gaaaagccct caaaaagctg ctgcgtcgga
       61 tggaagggct ccgccgtgac ctcgaacagg ccggacgata cgagccctac gcacgatacg
      121 gcgaactgtt gaaagccaat ctcggcttac tgaagaaagg catgaccgcc gtctcggtcg
      181 tggactatta cgatgagcgc ctacccgaat tgtcgattcc gctcgatccg accaaggggc
      241 cacaggccaa catggatgcg tacttcgcta aatatcgaaa attcgtctct gcccagcgag
      301 aaattgcgcc gcgcgtggcg gccatcgata cggacgtcca gcaactccag gccgaattag
      361 agaccatcaa aaatggaacc tggcgggcgc cgagtggtga acaccgaacc acgaccggaa
      421 caccgtctcg gcaggtccgg aagcgcggtg ccgacgagga acgccgggga ccgtttcgac
      481 gcttcatctc atcggacggc catccgatct ttgtcgggcg caacgcccgc gaaaatgacg
      541 aactcacgtt cggcctcgcc aagagcgagg acctctggct acatgcgcgc ggcacgccgg
      601 gctcgcatgt cgttgtgcgc ctggagaagg gcactgatcc gccgcctgag acgatacggg
      661 acgcagccac actcgccctg ctgtacagcg atctaaagaa aagcgggaag ggtgatgtca
      721 tgtacactcg acgcaaatgg gtcaagaaag ccaagggcca ggcaccgggc gcggtgaccg
      781 tcacccaaga gaaaaccatc tacatcaccc tcgacaaaac gcgtctcgcg gcgctcaaag
      841 cgagaaacac cgaaggaggg ccctagacga aacacttcaa cctgctccgc gccgcctttc
      901 cctcaatgtt cgagaaatgg tagagtgccc acatatgggc accggcacag acagacgaga
      961 tcgatttcgt attctgaatg cgctgctcca agcaaaaacg cttcggcagt tcgatgaacg
     1021 tctccagcag gaactcgcgc ccctcctggg ctgcgaaatc atcggcctct acctgtatag
     1081 tgaaagcacg gcggccttct cgcccgtctc ggaaagcctc cgcacaccgg ggtcgcccgc
     1141 ctatccgatc gatcagttgc ccgccgccgg caccatcaaa gaagcagccg tgctggccgg
     1201 gcaggccatc ttcacacacg acttgagtgc ctcgccctgg gccgaggcgc aggccatcga
     1261 ccccacaacc tgccaagcca cctcggtcct ggtgtcaccg atcaggatac cggctgaatc
     1321 ccagccctgc gcaaccgcca agaccttggc cgtgatggtc gccgtctcgc ctggaagacc
     1381 cggtctcttt tctgaagagg accgggcgtt tctcgacatg ctcggccttc atatcgcacc
     1441 ggtcctgact gcggtcctgg cggccgaaga acgcgacacc ctggtcgcca tcaacagcca
     1501 ggtcgtactg ggcaccatga ccctggataa tctcctgagc tcgatacagc ccattctccg
     1561 tgaggtcatc cctcacgaca cgaccggcct cgtgaggttc atcggcgccc cccacggccc
     1621 ctggttcgag atcctgtcct gtgaaggcgt cgccgtcgat caggaggcgc tgaaacaatt
     1681 tcccttcgag cggatggcgc cggccgaaat cctggccacc ggaaagcccc tgctgctcac
     1741 cggatacaac caggaacgat ttgcggaaca tacgtatttc gagtcactcg gcgtgttctc
     1801 tgccatgctc tgcccgctgc tcgtccgggg agcgccgtac ggctttctgg cgatcggaag
     1861 cagacggcgg aacgccttct ccgaacgcga cctggcgctg gccgaacaga tcggctttca
     1921 cttgtcgcac gcgatcgcca atctctcggc ctacgaccag atccgtcagc tcaaggatca
     1981 gctggaacag gaaaatgtct atctgcgtga ggagatgggt gcctctctcg acctgacaag
     2041 cctgatcggc aacagcgccg cgctgcagaa atcgctgaag gccattgagc aagtcgcgcc
     2101 gaccgattcc accgtcctca tcaccgggga aaccgggaca ggcaaggaac tggtggctca
     2161 agccattcat cgactctcgc cgcgccaaca caaggcgttg attaccgtaa actgcgccgc
     2221 gctcccgccc acgctcatcg aatccgaact ctttggtcac gagcggggcg ccttcaccaa
     2281 cgcaacatcc cgcaaattgg ggcgctttga attggcgcac gggggcacga ttttcctcga
     2341 cgaagtggga gaacttccga tcgacctaca aatgaaactc ctgcgcgtgc tggaggccca
     2401 ggaattcgac cgggtcggtg gaagccacac cattcgagtc gatgtacgcg tgcttgccgc
     2461 caccaacgtc gatctggatc aggccatcaa acgaggctca tttcgcgccg atctcttcta
     2521 tcgcctcaac gttttcccgc tgcgccttcc gaccttgcgc gaacgccgcg aggacatccc
     2581 gctactggcc cgccactttg tgaagaaata cagccagcgc cacagaaagc cggtcacgcg
     2641 aatcaacagt gcggccctga acgccctgtc cggctatgac tggcccggga atgtccggga
     2701 attggaacat gtgatcgagc gtgccgtcat cgtgagccaa ggctcggcga tcacggtcga
     2761 tgaactggat ggattgggcc gcggagaagc accctctccc gagccgcgga cgctcgcaga
     2821 agctgaacgt gcgcacatcg ttgagacact ctctcgaacc aattggatcc tggcgggcaa
     2881 gcaaggagcg gcggagaaat tgggactgaa acggtccacg ctccaacacc gcatgaagaa
     2941 gctgggcatc agccgcccgc ctcgacaggc ctcgtgagtg cccgtatgtg ggcatgcacc
     3001 tctaagcctt gcccaactct cggcacttcg ccctaatcat ctactccggt cacgtttctc
     3061 gattactctc tattctcctt atcttttagt aacttgctcg caaaagtgac tccgcattcc
     3121 gcgctggcat cacccctgct ctggtattcc tacatgaaga acgtcggttc cagactgaca
     3181 ccagacaccg tcagccatga gattgaggcc tatgtcgccg cgcgacaggc cgaattgctc
     3241 ccgtcaggcg aggcaagcaa agacgggcgc cggttctcct ggtgcgggga aattctggac
     3301 tttctgaccg aaggacttgc ggagcgactc gccgccaaag gcattacctt caccgatgcc
     3361 tttccaggcc ccttacggct catggacgaa gaccaacaac cgaacggcca acatgtgcgg
     3421 cgtttctgga ccgtcgccct gcaccatggc tgtcccatcg ccaggatctg cacctatttt
     3481 tttcatcgac atgatcaagt gagccttccg caattgccgc gcgtggtggc ctatgccccg
     3541 accggcccgg ctcaggaaga gcacgaatga attacgtcgc cctgagaatg ctcttcggcg
     3601 atcgagcgaa atacctcatg ctgctgtgcg gcctcacctt tgcggtcatg ttgatcgttc
     3661 agcaaggctc aattttttgg gggctcatga tctggtccca atcgagtatc agtaatctca
     3721 acgtgccgat ctgggtcacg gatcccggca ttgcgcaggt cgatgaagtc aaaccgattg
     3781 ccgacaccac ggtcgatcgc gtccgtagca tccccggtgt ggaatgggcc gttcccttgt
     3841 ataaggggct cttacgggcc aggcttgcga acggcgagta tcaccaaatc accttgaccg
     3901 gactggacgc tgccaccttg atcggccgtc cagcggaagt gctggaggga cgattcgaag
     3961 acctccggca acctgacgcc gtggccctcg atcaatgggc cgtcgaacgc atgggtgggc
     4021 caacagtcat cacggtcggc acggtcttcg aactcaacga caagctggcc cgcgtcgtcg
     4081 ccattgccaa gacacaaaaa accttcacca acattcctgt cgtctacacc acctacgagc
     4141 gggccttgcg atatgtcccg cgagaacggc gcaccctctc ctacgtcctc gcgaaggcca
     4201 aagacagcgc gccggtggac gaggtgatcc aacgtattcg cgaccacacg ggactgggcg
     4261 ccttcaccgc cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctgacgg
     4381 cacaagaggg tcagctcccg ttcctggaaa cctggccgct gctcctgttg gtctttgttg
     4441 ccctgctcat gatctgcaca ttgtcggcgc tgatcagcat catcaaactg gcccggcttg
     4501 aaccggcaat tgtgtttcga tgaccgccat ggatggttcg tcacagtcac agggccgcac
     4561 aacggaaggc aacggagccg agcaaaccat cgccgtgcag gtccgaggac tcgtcaagtc
     4621 gttcgggtcc ggcgagacca ccgtcacggt gctcaagggc atcgacctcg acgtgtattt
     4681 cggcgagctg ctgctcctcg tcggagaatc ggggggagga aagaccacgc tgctgtcggc
     4741 catcgccgga atcctggata tcgatgccgg tgagctcgat gtgttgggag cctcactgac
     4801 caacatgtcg ccaggcacgc gcacccgttt tcgtgggcaa accatggggt tcatctacca
     4861 acaattcaat ctcctgcccg ccttgacggc ggcggagaat gtcgccattc cgctcttgat
     4921 caaaggcact cggaagaagg aggcgatctc acgagggcgg ctgatgcttg aacgtgtcgg
     4981 gctcggcgac cggaccgagt ttctgccgcg caacctctcc ggcggccagc agcagcgcgt
     5041 cgccattgcc cgggcgttgg tcaatgagcc gcaactgctg gtgtgcgacg aacccaccgc
     5101 cgcgctggac gggcccaatg ggcagaagat catggaattg attcgggatg tggggcgtgc
     5161 ccccgatcgc tgcgtcatcg tcgtcaccca tgacagccgg atttttcagt tcggtgatcg
     5221 catggcggaa ttgaccgacg gccgcattgt cggcattcac ccgattcaga gagaggcaac
     5281 cgcatgacca ttctcaatcg ctttagtgtc tggctcgctg tcgcaggcgc gatgctggct
     5341 gcctgggtcg tcctgaccgc cggcaagaac cagcctatgc cgacgccgat agtagagcca
     5401 ccccggtctc cctatgaaac gaccgtcgcc gcgaccggca ttatcgaagc ggccaacgaa
     5461 aacgtccgta tcgggccgcc gatggcggga ctggtgacga aagtgttcgt ggcggtcggt
     5521 gaccgggttc gtgagggcga tcccttgctt caattggacg atcgagacct tcgcgcgcaa
     5581 ctggtggctc gccaggccgc gattccaccg gcggaggccc aagtcgaaga gcagaaatat
     5641 cgcatcggcg atctcgacac gcagctcaag cgactgaaat cggttcggga cagccgggcg
     5701 gtcagcgaag acgacgtgaa gcgtacctgg tacgcctcgg aaatggcaaa acgcgcgctg
     5761 agccgctacg aggccaccct caaacaagtc atcgcccagc gcgacgaaac acagatgctc
     5821 ctggaccgcc tgacagtccg tgcgccgcgg gccgcgacga ttttgcaggt caatgtccgg
     5881 gccggtgaat tcgccctgac caccggtgcg accgagccgc tcatgctcct gggcgatatg
     5941 cagcaactgc aagtccgcgc agaggtcgat gaggtcaatg ctccattggt ggctcccaac
     6001 cgacccggga tcgcctacct gaagggcaat actgcccagg ccattccgct gacattcgtg
     6061 cgcatcgagc cttatattgt ccccaaaaaa tccctgaccg gagacaacag cgaacgggtc
     6121 gatacgcggg tcctccagat catctaccgc ttcgaacggc cggcgtttcc gatctatgcc
     6181 ggacagcagg tcgatgtgtt catcgaacgc gcaccggacg gaccaccggc accagcaccg
     6241 ggcagcgcac gttccgctga ggaaccgagc aaatgaaacg agtcacgtcc cgccgacagc
     6301 tcgccatttc caccgtcctc gttatgttgt ccctgtccgc cagcgggtgc ctacagggct
     6361 cgaactacca tcgtccaccg gtcgaaacac cggccgactg gacacgcatg gcttccggcc
     6421 cattggctgc cggagcgacc gatacgatgc cggaagcgga ctggtggcag gccttccaca
     6481 accaggagtt gacccagttc gtggaacgcg cgctcacaca aaaccacgac gtccgccgcg
     6541 ccgtgtcacg cgtgctggag ggacgggctt ctgttaccac cgccggcgcc ggcctctatc
     6601 cacaactgaa tgtgcagggc agctacagca atatcgtggt gtcaaaaaac accctggcag
     6661 gattgggctt agccaccggc caacaaccgg ggccgcaggt ctttgccaag cccggaagca
     6721 gtttcgatct ctggaacggg gcggcggatc tccgatggga actggatgtc tggggtcgca
     6781 tccgtcgcgg gatggaagcc gcctcggcag acgcgcaggc gatcgagcag gacgcccgcg
     6841 cgattgcgct gaccctgatt ggggatgtgg gacagtcata ctttcgaatc cgtgaactgg
     6901 atgagcaaat tgaaatcgcc caacgcgccc tgaccctcag acgggattcg ctcgacatca
     6961 ttaccaagcg ggcatcagtg ggcctggcct ccgacctgga cgtaaaacga acggaagttc
     7021 tcgtcgcgga aagcgcgggg cagatcccgg atctgactcg tctgcgcgcc gtcgaattac
     7081 accggttgga agtgttgacg ggatcggcgc cgggcagcgt agtcttggcg ccgacatcct
     7141 tgcggaaggt catcgtacag ccggaaattc ccgtcggcct cccgtcgcaa ttgttggagc
     7201 ggcgacccga catccttcag gctgaggcgt cgctcgtggc cgccaacgcc cggatcggtc
     7261 aggcgcgggc ctatttcttc cccacattgt ccatcacggg gcagggcggc ctacaaagcg
     7321 ccgagttcgc aaactggttc tcgggaaaca gcgcgaattt cagtatcggt ccatcggtca
     7381 cactccccat tttcctgggc gggaccaacg tcgcgagact ggaggcggcc gaatcccgat
     7441 atcaacaaat gttggaaggt taccaacaga cgatcctgct cgccttccgc gaagtagcgg
     7501 acctcctggt gtcgatacac tcacgcacag aacaactcgc gcgccaacgt gaacaggcga
     7561 cggcggctac agcggccgta aggctggcgg aggtacgcta tcgcaagggg ttggtgaact
     7621 accttgacgt cctcgatgcc caacgaacga tgctggccgc agaaacacag cttgcgcaga
     7681 ccgagcgcgc ccgcctcacc gacatggtcg gcctctacaa agccctcggc ggcggatggc
     7741 agacgcccgg caacaatctc tctgcgatcc cggccggctc cgccacgcat taagtaatcc
     7801 acacggccag tgttagtcct tcctccggcg cagtcagcaa tcgcattgcc ggctgcctca
     7861 cactcgcatc gtcaccacag cagggacttt caacttgaat ttttcgcgcg gatagggcta
     7921 tttgacggaa tgcaatccga atccttcaat agagttgcgg accaggcacc ggtcgcgcgc
     7981 tctcgtcaga ttcttcgtac gacttctcgg aaggagcctt tcatgcaaga cggtgtccgc
     8041 ggccattccc cggcacgccg ggtttctcct gccctcatac tgttggtact cgggctcacg
     8101 acgttcttcc cgacgtatac gtcggctgcg cccctcgaca agccggtctc caaacccggg
     8161 atggacgaca agccggggct ggcagaagaa cagattcttt cgactaccga taaaatcacg
     8221 caaccggtag acacagacgc ggaagcgatg cgccacaacg atctcggcgt ggcctttgtc
     8281 ttcaagggcg acctggggca agccatcgat gaattcaaac atgcgctccg actccagccc
     8341 aactacttcg ccgcccacct taacctggct aataccctgc tcgatgtcgg gcggaacgac
     8401 gcagcgatgg ttgaattcaa agaagccctg cgcctcaaac ccgacgaccc caaaacccac
     8461 aatgatctgg gagtcgcgct caaggaaatg ggaaacttag aaggcgccat tgcggagttt
     8521 cgaacggtct tgcgccataa ccccagcgat gtgaacgcgc acaacaatct cggcgtgacg
     8581 ctcaaagcca tgggtgatct cgatggagcc atcgccgagt atcgcaccgc tgcgagcctt
     8641 caaccgaacg acgtgaacgc gcactttaat ttagggctgg gactgatgga aaaacgccag
     8701 ccggaagcgg ccgtcagcga gttccgcacc gcgctgcacc tgcggcccaa cgatgcgaag
     8761 atccgtctca acctcggcaa tgccctggca ggcatgggac agcggatgga agcggctcag
     8821 gaattgagac agtaccttcg ccttgaactt gatacgcccg ccaatcgtcg gtggctggaa
     8881 caggccgaag ccaaactccg tgaactggag atgccgtaac cggcgcctcg ctggccggtc
     8941 cagcgaggcc ggaatccgaa tccgggagtc atcgccgatc cgactgaatg gtctcactct
     9001 cctctcgccg atcccacctc atttggtgta cagtcctcaa cgcttccgtc ctgacgggaa
     9061 actttgatgc acaaccattt cactgggccc accatcggca gattcacgca cgtgagaggc
     9121 tggatcctgg ccgtcctgtg cgggatacct ctgtggcccg gccaatccct tccggcagag
     9181 caggaagatc cggagacatc ccacaccagg caatgggatt ttgactccat cgctccggga
     9241 acgctcccca gttcgttcgt gatcggcacg ctcttcgacg gccgcccggc cggcgagtgg
     9301 aaaattctca tcaccgaccg cgcgaagagc ccctcccaag tactcgctca actgcaaccg
     9361 aaaggaacag atcaggcgca caagctgctg ctcatggagg ggaccgacag cggcaacatt
     9421 gatgtcgagg tctcctattt ggccgtggca ggaaaagccg atttcggcgg cggacttgta
     9481 tggcatgcca cggatgaccg caattactac ctcctgaggg ccagctcggt tgaacagaag
     9541 gtcaggctct atcgcgtcgt gaagggggtc cagcaaatcg tcaagcagct cgaccgtccg
     9601 ctccccgcaa acgggtggca caaattgcgc atcgtacaac gtggctgcga gctcaaagcc
     9661 ctctatgacg atgccgtgct ctttcgcgtg tgcgaccaga cgttttccaa cggacggatt
     9721 gggctctgga cgaaagccga tgctgtgacc tatttcgacg acctggtcct tcggctcctc
     9781 gatgaccccc tctcgaacgg tcgtcccccg cgctgagcag ccaccgcacc ttcaccggct
     9841 accggcaggc acaaaaaagg ggcagccacc acaaagatga ctgccccaca agctgatacg
     9901 aaattaggcg ttaccagcga tcgcgcttgc ccccgcgatc gttgaatccc ccgccgccgc
     9961 tccgcccagg gccaccgcca gatcggggct cctgaggacg cgcttcattg accgtcaatg
    10021 tccggccacc catgtccgtc ccattgagag
//
LOCUS       DAOQRM010000088        10016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6049_length_10016_cov_12.150587, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000088 DAOQRM010000000
VERSION     DAOQRM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6049_length_10016_cov_12.150587"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            890..4477
                     /locus_tag="PKK81_08355"
     CDS             890..4477
                     /locus_tag="PKK81_08355"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNV32927.1"
                     /translation="MKMMRGSRLFAGGATRLPCVCATMFACWWLIPLGLPDTSHAQVT
                     PPITSSGLNTHISKPSGNSLQYDITAGTRAGTNLFHSFGDFSVPTNNIANFLNDSGLA
                     TSNILGRVTGGNPSHIFGTLQTTGFGGANLFLMNPAGIVFGPNAALNVGGSVSFTTAD
                     YLRLTDGARFNAIAGPQDAAISSAPVAAFGFLGSNPAAIAVQGSQLAVAEGQGISLVG
                     GNLTIQGGTLADTTIQPARLSAPGGPINLIAVASAGEVLTNAAAAPADPTLNGFTSLG
                     TISLTQGADVHTSAHTAGRIVIRSGQFTMENARLEASATATAPTSPPSASATGGISVQ
                     ADNVALSHSSNVLTSAVDGTAGNITFEVSTLRSNVGADGIPLSGAAPVTIASTSTGQG
                     GAGSIFFAGKAGEPADAVLLSHTHIVTGVTNAVDPTVVPGNIAMTAQRVELANGTAVR
                     ADTTGGADAGAITLNVDTLKTQSGPDGRVLISSDSHCGNQCVGGQAGYITLQGIPGFT
                     PPSTRLYRHVTAPDSEPNRAITYYFAREFDLRGTDIHSDAIGNAPGGIVMMRSNGQAS
                     LIDSGVSVTTQDFTINDNKPNGQPAQNQGFSRIDIMGRDIVLKDSTIKANAEVSDIGS
                     CPLCQGGPSAGEIWLRAEHSFTADNSLIANTGHGRAQAGITKIIKDHYFSFGAVWDAP
                     YPDAPIDMVKLTNSEVTVEALHEGLPGYLRIRGDTIILDHSVVNSQVNNVTNVLDSQG
                     RLIDVVGAGEAGTVVTDGRSVQGSLLMSAKNLDITGGGIIAPTQGNRIANRIELHADV
                     LTTRPGTRAGGTLAAPRILDVADPSRVVISSSSGSTGGAGMISITGVSGPIPDDVPYP
                     PSSTIRLTGTDVLTDTSSIGLGGRIEMRARGPIELHNTNISANVTDLRPQSVNGQEQS
                     GNIDLSAGNILMEGGGISALTRGTRSGGNVSLSAAQSVTVSDGAAISASSTGLGNAGN
                     ITINSGPRFLAQNASITTEANQASGGDIVIQAIDSIRFINGRLSTSVLGGPDTSGGNI
                     TLDPAVVTLQNSHILAQAVQGQGGNITIIAGTFLADPTSVISASSQFGLSGAVNIQSP
                     VSSLSGSLATLPQQPLQGQHLLRQRCAAQAGGQLSSLVIAGRDALPAEPGGWLMSPLM
                     FADEGLTQSAQMPTFESAHQPPVRSEQPVDQFGPLPQLGAGDWPADCRS"
     gene            4579..6276
                     /locus_tag="PKK81_08360"
     CDS             4579..6276
                     /locus_tag="PKK81_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNV32928.1"
                     /translation="MGVSATAQIAPPLPPIFDPTGRSGKPPAPLREEFKAPETPPPSL
                     ILPEVSPAPHKPFENRLGSVRVFVHDIHVTGSTVFSEAELADVIAPYRNRELTTEDLE
                     RVRLALTLLYVNRGYLTSGAVIPDQDVTFGTIVIQIVEGTLTRIDVEGNQWFRSSYLR
                     DRVARGIDTPVSIQPLQERIQLLQQDPRIERINAELRPGDVRGESVLNLRVKDANPFK
                     AWLEFNNYQTPVVGAERGLATVAHQNVTGHGDQFVFTYGRSHGVNPIIDTSYSVPLNS
                     YETTLTAYYRRNAFLVVENPFRALDLNVDSEVIGIAIRQPVYRTVSDEVALSLGGEHL
                     YLKTTSAFDVPGLPSLFIPGSSTTGVATVSALRFSQEYTHRSPSFVFAALSRFSVGLN
                     VLGATVNNGPLPDGQFVSWLGQAQAVKRIEKWWGIQLLGRVAAQIANDRLFPLEQMPV
                     GGRFSVRGYRENTLVRDDAVLASFESRVPLLRFASGEDRLQFAQFVDFGRAWNAKGHT
                     PDPHTLASVGVGLRWTILPQERARFEVYWGQQLNHVRGGEGNMQDHGIHLQLVVQAL"
     gene            6307..8619
                     /locus_tag="PKK81_08365"
     CDS             6307..8619
                     /locus_tag="PKK81_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNV32929.1"
                     /translation="MASRLRASLQPVLRVGLILLLSGTAASAAETGPSADELMQRGLS
                     AAERGALEQAHVDWKAAAQLYDQAGQVKGHLQALFHAAYAARALGHVNQAFLQQELAL
                     QLARRIGDPQWLALTLSELGKTYVTSHQYDTATDYLSQAAEIAHTRNLKTLSAALRND
                     LGIVLALQGRFPEALAAFQDSAAGATEENVRILFVRTRVNAARVAMQLKRFDEAAASL
                     NEADDRLAATAGSHNKADELINLALGYRDLATVQPDEHARWLKRSAALLQEAAAMAED
                     SGGQRLASYAYGHLGHLYEEERRLDEALQLTRRAVFAAQSAGAPESLYRWQWQSGRLL
                     TRLGSLDGALSAYQEAAATLQPIRAEVAFGSQASLDPAHQSIRSLYFELADLLLQRAA
                     LMTEDSEADSYLKAARDAIEAYKAAELRDYFRDDCVDQIQARLTKLDAVSPATAIIYP
                     IMFSDRTELLVSFPDGLRRHSVPVTASALTAEIRSFRRMLEKRTTREYLPHAQQLYDW
                     LLRPLLADLRRLKVNTLVFVPDGPLRTIPMSALHDGSQFLIAQYAVAMTPGLDLTDPR
                     PINRTRAQLLSSGLTKAVQGFPPLPHVAEEMAHLNSLFKGEQLLDSNFVTPRLEGELK
                     DGRYSILHIATHGQFATDVNQSFLLTFDDRLTMTQLERLVGLLRFRQDPLELLTLSAC
                     QTGIGDDRAALGLAGIAVKAGARSALATLWFINDEASSELVSEFYRQLHDSSISKAHA
                     LQIAQLKLLSGRLYEHPAYWSAFLLLNNWL"
     gene            8726..9433
                     /locus_tag="PKK81_08370"
     CDS             8726..9433
                     /locus_tag="PKK81_08370"
                     /inference="COORDINATES: protein motif:HMM:NF017829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HNV32930.1"
                     /translation="MAVKVGTTVLLAALLASSSEAGTDVQPEIPLYKAPQQVMPRARI
                     GGTLRGSEGEDPAIAALVPDHVGITVKQHPVLNWFLSKHTALPIKFTLIDERSIRALV
                     ERLITPPNQAGVHAVNLEDLGFTLQAHIQYRWYISVIKDENSPSRDIVTGGMIERCEF
                     SECMVLGATTTCTQEAVRTSAATGFWYDAMACLCDLITSDPGDTGLRKQRAALLKQVG
                     LHGVAEWDLAQTHAGQR"
     gene            9473..>10016
                     /locus_tag="PKK81_08375"
     CDS             9473..>10016
                     /locus_tag="PKK81_08375"
                     /inference="COORDINATES: protein motif:HMM:NF017829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HNV32931.1"
                     /translation="MLYLRLTLLVVVAMAWPVSWHSAVGLSSTLPDGRAADLVTAPDS
                     PMPLYKPRKNHAPRARIGGQSRGAESGAPLLIALVPDHVAFTIKGDPSLCWYLSLPTT
                     HAMTVTVVDSRGIRPILERALPSPLHAGIHCTRPREYGIELKEQESYRWFITLVVDPD
                     EPSRDIVAGGTIERIPYSEAC"
BASE COUNT         2084 a         3102 c         2831 g         1999 t
ORIGIN      
        1 atcgtgccca tccagcgctg atcagaccgc cttctccacg ccatctgccg cgtctccgcc
       61 atcacaggag acgcggcaat ctctctccag gccggcagtg taaccctcct ctcctgctat
      121 tggacgatct catcgtccgg catcgtcctc ccgtgtggac ggtatcgttt cacattcatc
      181 gaagcgatca tttcgtatca gattggatac ggcatagcat ccgattcgat acaccttacg
      241 gcagtacgta catctactcc agaagaacga gactgatcgt gatatcggat gtcatcaaaa
      301 tcactacgca tcttgccatc gaaaatcacc gatgctttcc gtcaccctcc atggcacaac
      361 ctttgcgcat ccacgaggca gataggatgt ttccctagga agtacgccaa cgggagaaca
      421 gcggatccat gaggtcacca tgctcgtgct tctttttctc ctactcggtg tcggcttcct
      481 tctcgcagct gtgggacgca tttggatgca tccaagccct cttcgctcaa gagcgaccga
      541 ttcgacggga atggatcacc tcaatgagtc tcgctcacgc aaagagatgt ccaagaaccc
      601 taactcctct tcgtaaccag ggaacaggtc ccggcagggt gtcggagtag tgcgcgggct
      661 gtgaagatgg ctgtatacgg cgggaagaga ggctcgtgcc gatggggtgc caaaaaccgg
      721 cattgcaccg gattgccggt cgagagtatt tccctagccc acatgatcga cctatgcggc
      781 cgggcaatcg cgtagagtat agcgaggtct gaacgaatgg agtcgcccta caccgtgcgg
      841 aaatgggccg ggtagtcctg accgcttgca cctgagatgc acaattgcca tgaaaatgat
      901 gaggggcagc agactcttcg ccggcggcgc aactcggctg ccctgtgtct gcgcgaccat
      961 gttcgcctgc tggtggctga ttcctctcgg cctgccggac acgagtcacg ctcaagtcac
     1021 cccgccgatc acatcctcag gactgaatac ccacatcagc aagccttcag gaaactccct
     1081 gcaatatgac atcaccgccg gaacgcgagc gggaaccaat ctgtttcata gcttcggaga
     1141 cttcagcgtt ccgaccaaca acatcgccaa ttttctcaat gacagcggcc tcgcgacctc
     1201 caacatcctc ggccgtgtga cgggcggcaa tccctcgcac attttcggca ccctccaaac
     1261 gaccggcttc ggcggagcca atctgttcct gatgaatccc gccgggattg tctttgggcc
     1321 gaatgccgcg ctgaatgtcg ggggatccgt gagtttcacc accgctgact atctgcgttt
     1381 gacagatggg gcgagattca acgcgatcgc cgggccgcag gatgcggcca tctccagcgc
     1441 accggtggcc gcgttcgggt ttttggggtc taatccggca gcaattgctg tgcaaggcag
     1501 tcagcttgcg gtcgcggaag ggcaaggcat ctcgctggtg ggaggcaacc ttaccattca
     1561 aggaggaacc ctcgcagata ccacaatcca accggcccgg ctttcagccc ccggagggcc
     1621 aatcaacctt atcgcagtcg cttcagccgg cgaggtgttg acgaacgcgg cggcggcacc
     1681 cgccgaccca acgttgaacg ggtttacatc actcggaact atttcactca ctcaaggagc
     1741 cgatgttcat acgagcgctc acactgcagg gcgcatcgta atccgcagtg ggcaattcac
     1801 catggagaac gcccgccttg aggcgagcgc aacggccacc gccccgacct caccaccctc
     1861 tgcttcagcg acaggaggga tctctgtcca ggcggataat gtggcgctgt cccacagctc
     1921 gaatgtcctc acctccgccg tcgacggtac ggcggggaat atcacctttg aggtgagcac
     1981 gttgcgcagc aatgtcggag ccgatggcat tccgctcagc ggcgccgcac cggtcaccat
     2041 tgcgagcacc agtacggggc aggggggagc cgggtctatt ttttttgcgg gaaaggcagg
     2101 ggagccggct gatgccgtgc tactgagcca tacccacatt gttacaggcg tcaccaatgc
     2161 cgtcgaccca acggtcgttc ccgggaacat cgcaatgacg gcccaacggg ttgagttggc
     2221 aaacgggacc gccgtacggg cagacaccac aggcggagcg gatgccggtg cgatcacgct
     2281 gaatgtggat accctgaaaa cccaatccgg tccggacggg cgcgtcctga tttcaagcga
     2341 tagtcactgc ggcaaccaat gtgtcggggg gcaggccggc tatattactc ttcaaggtat
     2401 cccaggcttc acccctccga gtacgcgact atacagacac gtcaccgccc ctgattcgga
     2461 gcccaacaga gccattacct attatttcgc acgcgagttt gacttacggg gcacggatat
     2521 tcattccgac gccatcggca acgcgccggg tggaatcgtg atgatgagga gcaacgggca
     2581 ggcatcgttg atcgactccg gcgtctcggt cacgacccaa gacttcacca tcaacgacaa
     2641 taaacccaat gggcagcccg cgcaaaacca gggcttcagc cgcatcgata tcatgggtcg
     2701 ggatattgtc ttgaaagaca gtaccatcaa ggccaatgcc gaggtgtcgg acattgggtc
     2761 atgcccgctc tgtcagggcg gcccgagtgc aggcgagatc tggttgcggg ccgaacattc
     2821 cttcacagcc gacaactccc tcatcgcgaa tacgggacat ggacgggctc aggccggcat
     2881 caccaagatc atcaaggatc actatttttc attcggcgct gtttgggatg ccccgtaccc
     2941 ggatgctccc atcgatatgg tgaagttgac caattcggag gtcacggtcg aagcgctgca
     3001 cgaagggttg ccgggctatc tcagaatcag aggcgacact atcatcctcg accattccgt
     3061 cgtgaactca caagtgaaca atgtgacgaa cgtcctggat tctcagggcc ggttgatcga
     3121 tgtcgtgggc gcgggggagg cgggcacggt tgtcacggac ggccggagcg tccagggcag
     3181 cctactcatg tctgcgaaga acttggacat cacgggagga gggatcattg cccccacaca
     3241 agggaatcgg attgccaacc gcattgagtt acacgcagat gtcttgacca ccagacctgg
     3301 aaccagagca ggagggacgc tggcggcacc caggatattg gatgtcgccg atccgtccag
     3361 agtggtcatt tccagcagca gcggcagcac cggcggtgcg gggatgatca gtatcaccgg
     3421 cgtcagtggc cccattcctg acgatgtccc ctaccctcct tcgtctacca tccgcctgac
     3481 aggaaccgat gtgttgacgg acacgagcag cattggtctc ggaggcagaa ttgagatgcg
     3541 cgcaaggggc ccgatagaac tgcacaacac gaatatatcc gccaatgtga cggatttgcg
     3601 gccgcagtcc gtgaacggcc aggaacagag cggcaatatc gatctatccg ctggaaatat
     3661 cttgatggag ggtggtggaa tttccgccct taccagagga actcggagcg gcggcaacgt
     3721 ctcgctgagc gcggctcaat ccgtcacggt gagcgacggg gcggccattt ctgccagcag
     3781 cacaggcctt ggtaatgccg gcaacattac catcaattcc gggcctcgat tcctcgctca
     3841 aaatgcgtcc atcacgacgg aagccaacca ggcgagcggc ggcgacatcg ttattcaggc
     3901 gatcgattcg atccgattca tcaacggtcg actcagcacc tcggtgctgg gcggccccga
     3961 tacatccggc gggaatatca cgctcgatcc ggcagtcgtg acgctccaga acagtcacat
     4021 cctcgcccaa gcggtacagg gccaaggcgg caacatcacc attatcgcgg ggacattcct
     4081 ggccgatccg acgagcgtca tcagtgcctc gtcacaattc ggcttgagcg gtgccgtgaa
     4141 cattcaatcg cccgtgtcca gtttaagcgg cagcttagcc accctgccgc aacaaccgct
     4201 acaagggcaa catctcttac gacaacgctg tgcggcgcag gccggcggac aattgagcag
     4261 tctcgtgatc gcgggaaggg atgccctgcc tgcggaaccg ggcggctggc tcatgagccc
     4321 cctgatgttt gccgacgaag gcctcaccca atcggcacag atgcccactt ttgagtctgc
     4381 gcatcagccg ccggtgcgtt cagaacaacc ggtagatcag ttcggtccgt taccacaact
     4441 cggtgccggt gactggccgg cagattgtcg ctcctgacct tgtgagcctc gcgcgcaggc
     4501 gaagagggag ccgaattgca ttgtgggatc tttcatatcc gatctgcgtg cataggcatt
     4561 acgatgagca tctgcctcat gggagtttca gccacagcac aaattgcgcc gccgcttcct
     4621 cctatttttg atcccaccgg ccggtccggg aaacctcctg ctccgttgag ggaagagttc
     4681 aaagcaccgg aaacaccgcc tccgagcctc atccttccgg aagtgtcacc cgccccgcac
     4741 aagccgttcg agaacagatt gggatccgtt cgagtgttcg tccatgacat ccacgtcacc
     4801 ggcagtaccg ttttttctga agcagagctc gctgacgtca tcgctcccta tcgcaatcgt
     4861 gaactaacca cggaagatct ggagcgggtt cgcctggctc tcaccctctt gtacgtgaat
     4921 agagggtatc tcacctccgg cgccgtcatt cccgatcaag atgtcacctt cgggaccatc
     4981 gtcattcaga tcgtcgaagg gaccctcacc cgaatcgacg tcgaaggcaa ccaatggttc
     5041 cgcagttcgt acctacggga tcgtgtggcg cgcggcatcg acactcccgt cagcatccag
     5101 cctctccaag aacggatcca actgctgcag caggatccgc gcatcgaacg gatcaatgcc
     5161 gagctgagac cgggagacgt tcgtggtgaa agcgtcttga atctgcgggt caaagacgcc
     5221 aaccccttta aggcctggct ggagttcaac aactatcaaa caccggtggt cggcgctgag
     5281 cgcggcctgg cgacggtggc gcaccaaaat gtgacaggtc acggcgacca atttgtgttt
     5341 acctacgggc ggtcgcacgg cgtgaacccc atcatcgata catcctatag tgtgccgctc
     5401 aactcctacg agacgaccct cacggcctac tatcggcgca atgcctttct ggtcgtcgag
     5461 aatccttttc gcgccctgga tctgaatgtg gactccgaag tcatcgggat cgcgatacgc
     5521 caacctgtgt accgtaccgt gagcgacgag gtcgcgctct ctctaggcgg cgaacattta
     5581 tatctgaaga cgacgtctgc gttcgacgtc ccgggtctgc cgtcgctctt cattcccggt
     5641 tcttctacga ccggggtggc caccgtcagc gcgctccgat tcagccagga atatacgcat
     5701 cgatccccat cctttgtatt tgccgctctg tcccggttca gcgtcggact gaacgtcctg
     5761 ggtgctaccg tcaacaacgg gcctctgccg gacggtcaat ttgtttcctg gttaggtcaa
     5821 gcccaggcgg tcaaacggat agagaaatgg tggggcatcc agttgttggg acgcgtggcc
     5881 gcacagatcg ccaacgaccg gctctttccg ctcgaacaaa tgcccgtcgg cggacgcttc
     5941 agcgtacgcg gctatcggga aaacacgctc gtgcgggacg atgccgtgct ggcatcattt
     6001 gaatcgcgcg tccccctgct ccggttcgcg tctggagagg accggctgca gttcgcacaa
     6061 ttcgtcgact tcggacgggc ctggaacgcc aaagggcaca cccctgatcc tcacacgttg
     6121 gcgagcgtgg gagtcggatt acgctggacc attctgcccc aggaacgtgc acgcttcgaa
     6181 gtctactggg gccagcaact caatcacgtc cggggcggcg aggggaacat gcaggatcac
     6241 ggtatccatc tccagctcgt cgtgcaggcg ctgtaaccga cccgactgcg gcagaaaggg
     6301 catgacatgg catcacggct tcgcgcttca cttcagccgg tgctcagagt cgggctcatc
     6361 ctcctgctct ccggcacagc ggcatccgct gcggaaaccg ggccgtcggc cgatgaactg
     6421 atgcagcgag gcctttccgc cgcggaacgg ggcgctttgg agcaggcaca cgtcgattgg
     6481 aaggcagccg cgcaactata cgaccaagcg ggccaggtga agggacatct tcaggcgctc
     6541 ttccatgccg cctacgcagc acgagcattg gggcatgtga accaagcctt cctgcaacag
     6601 gaattggcct tgcaactcgc gcgtcgaatc ggcgatccac aatggctggc gctcaccctc
     6661 tctgagttgg ggaaaacgta tgtgacgagc catcaatacg acaccgctac ggactatctg
     6721 tcgcaagctg cagagatcgc gcacacacgg aatctcaaga cactttcggc ggcgctccgg
     6781 aacgacctcg gcattgtcct ggcgttacaa ggccgttttc cggaagccct tgccgcattt
     6841 caagacagcg cggcaggagc gacagaggag aatgtacgca tactgttcgt ccgcacccgc
     6901 gtcaacgccg cgcgggtggc gatgcagttg aaacgatttg atgaggcggc agcatccttg
     6961 aatgaggccg acgaccggct ggcagcgact gccggttccc acaacaaggc cgatgagctt
     7021 atcaatctgg cgttagggta tcgcgacctg gcgaccgttc agccggatga gcatgcgcgc
     7081 tggctcaagc ggtccgctgc gctactgcaa gaagcggctg cgatggcgga ggattccggc
     7141 ggtcagcgcc tggcctccta tgcctacggg cacctcggtc atctctatga ggaggaacgc
     7201 cgcttagacg aagccctcca actcacccgg cgagccgtct ttgctgcgca gtctgccggc
     7261 gcaccggaat cactctaccg gtggcaatgg caaagtggtc gtctcctcac tcgtctgggc
     7321 agcctcgatg gcgcattgtc ggcctatcaa gaagcggcgg ccaccctgca accgatccgc
     7381 gccgaagtgg ccttcggttc gcaagcctcg ctggatcctg cccatcaatc gattcgttcg
     7441 ttatatttcg aattggccga tctgcttctg caacgggcgg ctttgatgac agaggactct
     7501 gaggccgact cctatctaaa ggcggcgcgc gacgcaatcg aggcctacaa agccgccgag
     7561 ctccgggatt attttcgcga tgattgcgtg gaccagatac aggcccgact gaccaagctg
     7621 gatgccgtat cccctgcgac ggccatcatt tacccgatca tgttcagcga tcgcaccgaa
     7681 ctgctcgtga gcttcccgga cggcctcaga cgacactccg ttccggtgac ggcgtcggca
     7741 ctgactgccg agatacgcag cttccggcgc atgcttgaaa agcggacgac ccgcgagtac
     7801 ctgccccatg cgcagcaatt gtatgactgg ttgcttcgac cgcttctagc ggaccttcgc
     7861 agactcaagg tcaacacgct ggtctttgtc ccggacggac cgcttcgcac cattcccatg
     7921 tcggcgctcc atgacggaag ccaattcctc atcgcacaat acgccgtcgc aatgaccccc
     7981 ggcctcgatt tgactgaccc acgccccatc aatcgaacca gagcgcaact actctccagc
     8041 ggtttgacca aggcggtgca gggttttcct cctctcccgc acgtcgccga agagatggcc
     8101 cacctcaact cgctcttcaa aggcgagcaa ctcctcgaca gcaactttgt cacgccacgc
     8161 ttggaaggag aattgaaaga cggccgctac agcattctcc acattgccac gcacggccag
     8221 tttgccacgg acgtgaatca atcattcttg ctgacgttcg acgatcggct gaccatgacg
     8281 cagttagagc ggctggtagg gctgcttcgc tttcgccagg atcccttgga actcctcacg
     8341 ttgagcgcct gccagaccgg tatcggagac gaccgtgccg cgctcgggct tgccggcatt
     8401 gcggtgaaag ccggagcgag gagtgccctc gcaaccctct ggttcattaa cgatgaagcg
     8461 tcgtcagaac tggtatcaga attttatcgc caattgcatg actcatcgat atctaaggca
     8521 cacgctttgc aaattgcaca gctgaagtta ctgtccggcc ggctctatga acatccggcg
     8581 tattggtcgg catttctgtt attgaacaat tggctctagc aggatgttaa aaacgttcgc
     8641 catcagcgtt ctcgcatcgc tcgggccctc accgtgccct gaagggtacg cctcgccccc
     8701 tcgctcgttg cggcgttgct tgtgcatggc cgtgaaggtc ggcacaacgg tgctgctcgc
     8761 ggccctgctc gcgagcagca gcgaggcggg aactgacgta caacctgaaa ttccgctgta
     8821 caaggcgcct cagcaagtga tgcccagagc tcgaatcggt gggaccttga gaggcagcga
     8881 aggagaagac cccgctattg ccgcgctcgt tcccgaccac gtcgggatca ccgtcaagca
     8941 acatccggtt ctcaactggt tcctatccaa gcacacggcg ttgccgatta aattcaccct
     9001 gatcgatgag cgatcgattc gcgcgctcgt tgaacggctc atcacgcccc cgaaccaagc
     9061 cggtgttcac gccgtcaacc ttgaggacct cggcttcacg cttcaggcac acatccaata
     9121 ccggtggtat atctccgtga tcaaagacga gaactctccc tcacgcgata tcgtgaccgg
     9181 cggaatgatc gagcgctgtg agttcagcga gtgtatggtg ttgggcgcca cgaccacctg
     9241 cacccaggaa gcggtgagga cgagtgccgc cacgggtttc tggtacgatg ccatggcctg
     9301 cctgtgcgat ctcattacct ccgatccggg agatacgggt cttcgaaaac agcgggcggc
     9361 cttactcaag caagtcggct tgcatggcgt ggccgaatgg gatcttgcgc aaacccatgc
     9421 cggccagcgc tgacaagctg cgcgagtccg ttcaactact aatcggcaca gaatgctcta
     9481 cctcagactc accctgttgg ttgtcgtggc tatggcgtgg ccggtgtcct ggcacagcgc
     9541 cgtcgggctt tcgtcaacac ttccggacgg tcgcgcggcc gacctcgtca cggctcctga
     9601 ctcgcctatg cccctgtata aaccgcgcaa gaaccatgcg ccgcgggcgc gcatcggcgg
     9661 gcagagcagg ggtgccgagt cgggagctcc cctgctgatc gccttggtcc cggatcatgt
     9721 cgcctttacg atcaaaggcg atccgtcact ctgttggtac ctgtctctgc ctaccactca
     9781 cgccatgacc gttacagtgg tggactcccg cggaattcgg cctattcttg aacgcgcgct
     9841 cccctcacct cttcatgcag gcatccactg taccaggcct cgggaatatg gaattgaact
     9901 caaggagcag gaatcctacc gatggttcat cactttggtc gtggaccctg atgaaccttc
     9961 gcgggacatt gtcgcaggcg gaaccatcga acggatcccc tatagtgaag cctgct
//
LOCUS       DAOQRM010000089         9901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6164_length_9901_cov_16.416108, whole genome shotgun sequence.
ACCESSION   DAOQRM010000089 DAOQRM010000000
VERSION     DAOQRM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6164_length_9901_cov_16.416108"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..489)
                     /locus_tag="PKK81_08380"
     CDS             complement(100..489)
                     /locus_tag="PKK81_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32932.1"
                     /translation="MEQPTGSSGQRSGKRPTVAVVYEDVTTGYKCADWFALHGYQATM
                     TSAVHHLERNLKELRPDVIVVRLSSAPTPLHSTVPRLKMTCPQVPIIAMVDQSTDALD
                     GVAPDLTKAFGADVFMCDSLDPPSPPS"
     gene            complement(883..1530)
                     /locus_tag="PKK81_08385"
     CDS             complement(883..1530)
                     /locus_tag="PKK81_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV32933.1"
                     /translation="MRTTGTVRILVVDDHPSFRRGVKDILEEGFEGASMAECGNAQEM
                     LDHVREQPYDLVVMDISMPGRSGPEVLKELKQLAPTLPVLILSMHPEDQYAIRMFKAG
                     AAGYLTKASAPEELVHAAKKVMSGGQYVSASVGEALALTVRTGVDKLAHERLSDREYE
                     VLCLIASGQTVSDIAESIHLSVTTISTYRARILDKMNLKNNAELTRYALQHGLVV"
     gene            complement(1562..4717)
                     /locus_tag="PKK81_08390"
     CDS             complement(1562..4717)
                     /locus_tag="PKK81_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV32934.1"
                     /translation="MYSLARFSLLEMTECSAVLRQLGEQSSSLQDAASRAVDYLFRTL
                     GNPDTGARDCVLVRCFKTVSYNRLNPMTQQRVREQLGGIAPFPDLKCFTLMATAGEEA
                     EWNQVERSHRYRAIPMVSADFVKQFPMFSQLLHQFGLSSSFDVAPEGEWLVDLEEKTY
                     NVFYVPDAVGSPYVPVQEGFVLKYGVKSVLCFGGMLPSRDLFAVILFSRTPVPRDTAA
                     LCKSLALSLKTSLLACDETVPLEAGVDAHSSSAPAIGAPEQGAGLRHQSRLAVAEQLL
                     RVYENAVRTQSAGIAQAKQALWERANALERSEQALSEQTRIVNSVLRSMGDGVVVTDA
                     EGQLVVANPAVESILGFSPFDIPLTAWAERYEFFHADTVTPFGQDEWPLARALRGEKI
                     DWLEVYLRASQGMPGRWISINARPIKDEAGVLSGTVVVFHDITWLKRAQDALFESEYR
                     FRSLVEGARDIIFTLSADITITSLNPAFETVTNWSRSQWMGEPLVALLHPDDAGFCQD
                     VLQAILERGAPLTCSMQISTKQGGYVTGEFVGTPQMRQGRVIGVLGIIRDVTERRRIE
                     DALRVSEERLRSIVQSTKDAIVLVNALMKVAFWNKGAEATFGYSAEEIIGQPVTMIIP
                     EQYHEELERNVQRVRLFERVQLTSRTLELFGRRKDGGEFPLELSVTSWKGKSDLFFTI
                     IMRDISERRSAEEELDRLHHHNQVVLNSAGEGIYGVDRDGRLTFVNPAAAKMFGWEAE
                     ALIGQPFSTLVHRPDFREGASGERLSPIVETIQGGEIREEADSRFWRRDGTSFPVEYV
                     STPIRERGDIVGAVVVFKDTTDRKRAEEQLQDSLRRLRKLSGRMEGIREEERGRIARE
                     LHDELGVGLTCLKIDLSRLGGLLGERLEPRDRAKVDEKIRGMKEQVDSTITSVQRIVA
                     ELRPGVLDDLGLVAAIEWQCRDFQRRTGVACHCTVSHEDLRVDPEHATAVFRICQEAL
                     TNVTRHAQATEVHVRLEDQGGGLLLQVSDNGRGIPSDRLADARSFGLLGMRERAGLLG
                     GDVQIETKEGSGTTIALQLPR"
     gene            complement(4981..5403)
                     /locus_tag="PKK81_08395"
     CDS             complement(4981..5403)
                     /locus_tag="PKK81_08395"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV32935.1"
                     /translation="MPEAGLVRSAGGVVFRQQDVLLIRVSDIKGRPVWSFPKGRLDAG
                     ETPAQAALREVLEETGWCCRIEADLSTTEYWFQREGRRFRKTVVWFKMSALEEAGVPD
                     GEVEEVQWVDREVALGRLTYPSDVALLSQALSQGPSSR"
     gene            complement(5454..6317)
                     /locus_tag="PKK81_08400"
     CDS             complement(5454..6317)
                     /locus_tag="PKK81_08400"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV32936.1"
                     /translation="MAVQGPIVITGASSGIGAACARYLDALGFTVWAGVRSTQDGEAL
                     TRLTSSRLRVLMLDVTDPASIEAARKTLAEATRDTGLAGLVNNAGISVAGPLELLPLS
                     EVRTQFEVNVIGVLAVTQALLPLLRLARGRIVNISSIAGLAATPFLGAYCSSKFALEA
                     MSDALRLELAPWGISVSLVEPGAIQSQIWQRATMSATRTLGGVEPEALALYAQPLSRM
                     QEVMAAAAARAIPAEVVARVVADALIAARPRARYLVGKDARFRAMLKWILPDRAQDRL
                     LAWFLGLPHRG"
     gene            6850..7269
                     /locus_tag="PKK81_08405"
     CDS             6850..7269
                     /locus_tag="PKK81_08405"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32937.1"
                     /translation="MSSIQRAFRFAVERLAPSVRPTGTLSLYARFSGPLNAEGATAEA
                     ALAIGFLALLKGDHLKQDITITGTLEGDGRIGQVAQVAEKTTAAARAGYHVLLVPRGQ
                     FYAPRVNRVSLNLESNVLVREVETIEEAYEIMTGKKL"
     gene            complement(7411..9885)
                     /locus_tag="PKK81_08410"
     CDS             complement(7411..9885)
                     /locus_tag="PKK81_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNV32938.1"
                     /translation="MPTKRNMVTRRGMKRRHGSAGPSATRALSSPLTMLGVGVLALSG
                     LAWPVSGIAMELFGGSGASSISPSQEMRQGTTQFQQGAFAQAAAHWMNAARGYEESGQ
                     AKEQCQALINLAHALQQEGQIRRAQGTLQAALKLSEQNGERGLTATILAQLGNTFHVL
                     GKDEPATEHLTRALTLAREENKPLLVAGVLNDLGNALTARRQFAEAIDVYAESRSLAI
                     ETKQPALAATAQINGAMALLQNQQLSEAHRHLDQAWSDVRSLGDGQAKTAGLLNIGLG
                     YQELFAATTAKSGVARKSDAGKGRATEAVASAGLGAGLLRQSSDAFIAAGEVAGRIGD
                     ARGQSYAWGYLGGLLEQEHRNPEALEYSRKATFAAQKVNAPESLYRWQWQTARLLRAD
                     GKEEEALAAYQRAVTLLKPIHYEYSVGYQGRHHSYYESVAPLFVEYEDVLLRRAAAAK
                     TPDQNEQLLVQVKDTVEVSHAAELQDYFQDDCVTTVASHRGVGTLAPGTAVVYPISFP
                     DRLELLLETANGLKQVRVPVAGERLTKEIRSFRRLIQDSQSQNYLSSAQTLHGWLVAP
                     LQQDLQGAGIHTLVMVADGSLRTIPMGALHDGRHFLVDSLAVAVTPSLALTDLSAAQR
                     RKGSLLSVGLTESVEGLSAPRYAESEVQAIRTLYGGKLLMNKQFSAPSLEEEIKDQGV
                     GIVHVASHTVVGTEARDSFVLAHDGKITMDRLSQLVGLQQYRQQPLDLLTLSSCEIAA
                     EDDRAALGLSGVAVKTGARTALASLWTSDDETTTELVSEFYRQLQDPALSKAVALQRA
                     QQKILSQRGHTHPSFWAAFLLINNWM"
BASE COUNT         2063 a         3293 c         2652 g         1893 t
ORIGIN      
        1 ctcctttcag ctggtgagac actggcacga tgggacggct gaccatcgtc gcactgtcta
       61 tgctaggcgg tgtctgagca agtcaggcgt ggaggcgtac tacgagggcg gcgacggcgg
      121 atccagggaa tcacacataa agacatcagc accgaaagcc ttggtcagat caggagccac
      181 cccatccagg gcatccgttg attgatccac catcgcaata atcggcactt gcgggcaggt
      241 cattttgagt ctgggaacgg tactgtgcag cggagtcgga gcggacgaca atctcaccac
      301 aatcacatcc ggacgaagtt ccttcaggtt gcgctccagg tgatggactg ccgacgtcat
      361 ggtggcttgg tacccgtgca gcgcaaacca atcggcacac ttgtagccgg tcgtgacgtc
      421 ttcgtacacc acggcgaccg tgggtcgttt cccggatcgc tgtccgctgc taccagtcgg
      481 ttgctccatc agttcaccct cgtctggatc agcttaggtt gtggttcgaa cgcctgtgag
      541 ttgtgtcaat cgacgcgcca tttgaggacc ccaccaggcg aggtcttgta acatcgcggc
      601 ttccacggcg gtcaatccgg tgaggatgcc gggccaaccc gggcgaaagg acccggttcg
      661 gcgatcaata ccagaacgct ctgccgatcc ctgtccctgc gcagcctcaa agaccgcaag
      721 caatgtcgtc gtagtggaga tccacggctt ctgcatgaag atgaggatag gcccgagaag
      781 aggaggatgc tatcggcaga ggtgcgattc ctgtgtcaca aatagacagt gcctcaatgt
      841 catggttata acgacatggg cgaggcgacc gaccccattc ccttatacga ccaacccatg
      901 ctgcaacgca tagcgcgtca actcggcatt atttttcaaa ttcattttgt ccagaattct
      961 cgctcgatac gtactgatcg tcgtcacgct gagatgaata ctttcggcaa tgtcactgac
     1021 ggtctgtccg gaagcaatca gacagagcac ttcgtactcc cggtccgaca gccgttcatg
     1081 ggccaacttg tcgaccccgg tcctgaccgt caacgccaag gcttctccca ccgaagcact
     1141 gacgtactgc ccgcccgaca tgactttttt cgctgcatgg accagctctt ccggcgcact
     1201 cgccttcgtg agataccctg ccgcgccggc cttaaacata cgaatggcat actggtcttc
     1261 cgggtgcatg ctcaagatca agacaggcag cgtcggcgcc agttgcttca gttccttcaa
     1321 aacttccggc ccgctccgtc ccggcatact gatgtccatg acgacgaggt cgtagggctg
     1381 ctcgcgaaca tggtccagca tctcctgcgc atttccacac tcagccatgc tggcgccctc
     1441 gaatccttct tccagaatat ccttcacgcc gcgacgaaac gaagggtgat catcgacgac
     1501 gagtatccgc acggtccctg ttgttcgcat agtgccctcc atcatgtcct ggtcctccgt
     1561 cttaccgagg cagctgcagc gcgatcgtcg tcccgctgcc ctccttcgtc tcgatctgta
     1621 catccccgcc gagcagcccg gcgcgctccc gcatccccag caggccgaac gacctggcat
     1681 ccgcaaggcg atcggatgga atgccacgac cgttgtcgct gacctgcaac agcaaccccc
     1741 cgccctggtc ttccaagcgc acgtgtacct ccgtggcctg agcatgacgg gtcacgttgg
     1801 tcagcgcctc ctggcaaatc cgaaaaaccg cggtggcatg ttccgggtcc acccgcaaat
     1861 cttcatgact gacggtgcag tgacaggcaa cgccggtgcg gcgttggaaa tcgcgacact
     1921 gccattcgat ggccgcgacc aggccaagat cgtcgagcac gccggggcgc aactccgcca
     1981 caatacgttg cacagaggtg atggtactat ccacctgctc cttcatgcct cgaatttttt
     2041 cgtcgacctt tgcgcgatcc cggggctcca accgctcccc cagcaatcca cccaggcggg
     2101 acagatcgat cttcagacag gtcaggccga cgcccaattc atcatgcaac tcacgtgcaa
     2161 ttcgcccgcg ttcctcttct cgaatcccct ccatccgccc cgacagtttg cgcaggcgac
     2221 gcaatgagtc ctgcaactgc tcctcagccc ttttccgatc cgtcgtatcc ttgaatacga
     2281 cgaccgcccc gacgatgtcg ccccgctccc gaatcggcgt actcacatac tccaccggaa
     2341 aactggttcc gtctcttcgc cagaaccggc tgtccgcctc ctcccgaatc tccccgccct
     2401 gaatggtctc cacaatggga ctgagtcgtt caccggaagc tccctcacgg aagtcgggac
     2461 ggtggaccaa cgtagaaaag ggctggccga tcaatgcctc cgcctcccag ccgaacattt
     2521 tcgccgccgc cggattgacg aacgtcagcc gcccgtcccg atcgaccccg taaatgccct
     2581 ccccggctga attgagcacg acttggttgt gatgatgcag gcgatccagt tcctcttccg
     2641 ccgatcgccg ctcactgata tcgcgcatga tgatcgtaaa aaagagatcc gatttcccct
     2701 tccaggaggt cacactcagc tcgaggggaa attcgccgcc gtctttccga cggccgaaga
     2761 gttccagcgt cctgctcgtc agctgcaccc gttcaaacaa tcgaacccgc tgaacattcc
     2821 gttccaattc ctcatggtac tgctcgggaa tgatcatcgt cacaggctga ccgatgattt
     2881 cctcggccga atagccgaag gtcgcttcag cgcccttgtt ccagaacgcc actttcatga
     2941 gcgcattgac caatacgatg gcgtctttcg tcgactgcac aatggagcgc aaccgctcct
     3001 cgctgacgcg aagcgcatcc tcgatccggc ggcgttcggt cacatcacga atgatgccca
     3061 agaccccgat gacacggccc tgtcgcattt gcggagtccc gacaaactct ccggtgacgt
     3121 acccgccctg cttcgtgctg atctgcatcg aacaggtcag cggcgccccg cgttcaagaa
     3181 tcgcctgcaa cacatcctga cagaaacccg cgtcatcggg atgcagcagg gcaacgagcg
     3241 gctcccccat ccattgcgaa cgcgaccagt tggtcaccgt ctcaaaagcc gggttgagcg
     3301 aggtaatcgt gatgtccgcc gagagcgtga agatgatatc gcgcgcccct tccaccaaac
     3361 tccggaatcg atattccgat tcaaacaagg catcctgcgc ccgcttgagc cacgtaatgt
     3421 cgtgaaacac caccacggtc ccggagagca caccggcctc atccttgatc ggcctggcat
     3481 tgatgctgat ccagcggccc ggcataccct gcgaggcccg taggtagact tcgagccagt
     3541 cgattttttc gcctcgcagc gcgcgcgcca gcggccactc atcctgcccg aacggtgtca
     3601 cggtgtccgc atgaaaaaac tcataccgtt cagcccatgc cgtcagagga atgtcaaagg
     3661 gcgaaaagcc cagaatactt tccaccgccg gattggcaac gaccagctgc ccctcggcat
     3721 cggtcaccac aaccccgtcg cccatactcc gcaggacgga gttcacgatc ctggtttgct
     3781 cactcaacgc ctgttcagat cgctcaagcg cattagcgcg ctcccaaagg gcctgtttcg
     3841 cctgcgcaat tcccgccgat tgcgtccgga ccgcattttc atagacccgt agcaattgtt
     3901 cggccaccgc caagcgggac tgatgccgga ggccggcccc ttgctccggt gcgccgatcg
     3961 ccggagccga cgacgagtgg gcatcgacac cggcctccaa gggaacggtc tcgtcgcaag
     4021 ccagcaacga cgttttcagg ctgagggcga gcgacttgca caaggctgcc gtgtcacggg
     4081 gcaccggtgt tctggaaaat aaaatcacgg cgaagagatc tcgggacggt aacatcccac
     4141 cgaaacacaa caccgatttc accccgtact tcagcacaaa cccttcctgc accggcacat
     4201 aggggctccc caccgcgtcc ggcacataaa atacgttgta cgtcttctcc tccagatcca
     4261 ctaaccactc cccctccgga gccacatcaa agctggagga aaggccgaac tggtgcaaca
     4321 gctgggaaaa catcggaaac tgtttgacga aatcggcgct gaccatcgga atcgcccggt
     4381 agcgatggga acgctcgact tgattccact ccgcctcctc accggccgtg gccatgagcg
     4441 tgaaacactt gaggtcgggg aacggagcga tgcccccgag ctgttcccga accctctgct
     4501 gggtcatcgg attcaaccgg ttgtaggaaa cggtcttgaa gcagcgaacc aggacacagt
     4561 cgcgagcgcc tgtgtcggga ttgccgaggg tgcggaacag atagtccacc gctcgcgatg
     4621 cggcatcctg aagcgaactc gattgctcgc caagctgacg caacacggca gagcattcgg
     4681 tcatttccaa cagggaaaag cgagccagac tatacacagg cgggccctcg ggtgaacgat
     4741 gggggaatac ggatcaacgc gccactggtt gacgttcgtc cggagaaaac gactcgtgag
     4801 ggaaggcgcg tctctgcaga gcactgcctt gcaaggcgag gcacaaagtc actctcgtgg
     4861 cgagtctagt aaagggggcc ggccatgtca aggcagacag gtgggttcag gctgaccgaa
     4921 acacccgaat catttcaagc gatcgcatct cacacccgtc tcgcacgcag accattgcgg
     4981 ctatcgggag gaaggcccct gggaaagcgc ttgagacaac aacgccacgt ccgatggata
     5041 cgtcagacgg ccgagtgcta cttcgcgatc gacccactgc acctcctcga cctctccgtc
     5101 ggggactccc gcctcttcaa gcgccgacat cttgaaccac acgaccgtct tgcggaaccg
     5161 ccgcccctcc cgctgaaacc aatattcggt cgtggagagg tccgcctcga ttcgacagca
     5221 ccatccggtt tcttccaaca cctcacgcaa cgcagcctgc gcaggcgttt ccccggcgtc
     5281 gagccggccc ttggggaacg accagacggg acgccccttg atatcggaca cacggatcag
     5341 caacacatcc tgttgccgga acaccacgcc tcccgctgat cgcaccaatc ctgcttcagg
     5401 cataacccgt tcttcccccc gccgcactcg acattccgtc tgacttactc catctagccc
     5461 cggtggggca atcctagaaa ccaggccaac aggcgatctt gagcgcgatc gggcaagatc
     5521 catttcagca tggctctgaa tcgcgcatcc ttgccgacca gataacgcgc acgtggtcgc
     5581 gcggcgatca atgcgtcggc caccacccgt gccaccacct cggccggaat cgcgcgcgcg
     5641 gcggcggcgg ccatcacctc ctgcatccgg gacaacggct gagcatacag agcgagtgcc
     5701 tccggctcga caccgccgag ggtgcgcgtg gccgacatcg tagcccgttg ccatatctgc
     5761 gactggatcg cacccggctc gaccagcgac accgagatgc cccacggcgc caattcgagc
     5821 cgtagcgcat cactcatcgc ctccaacgca aacttcgagc tgcagtaggc gccgagaaac
     5881 ggcgtggccg caaggccggc gatcgaactg atgttgacga tccgcccgcg cgccagccgc
     5941 agcaatggga gcagtgcttg ggtcaccgca aggacgccga tcacattcac ttcaaactga
     6001 gttcgcacct ctgagagagg caggagttcc agggggccgg cgacggaaat accggcattg
     6061 ttcacgagac ccgccagtcc cgtgtcacgc gtagcctcgg ccaacgtctt gcgggccgcc
     6121 tcgatcgacg ccggatccgt cacgtccagc atcaacacac gaagtcgtga ggaggtcaga
     6181 cgtgtcagcg cctccccgtc ctgcgtgctc cggacacccg cccatacagt gaaccccaac
     6241 gcatcgagat accgcgcgca cgccgcacca attccggagg acgctccggt gatcacaatc
     6301 ggtccctgca cggccatacg ttctgttctc cacgcagcac cgactgccat acatgcccgg
     6361 gcgagcggat gagcaagacg caagaatatc acgcgcctga ggcaagaggc atcatgggtc
     6421 cggatgaccc gcctcctcac gcatttcagt aggtaattcc agaatccaga aaaagagatg
     6481 agtgttttct gatatttacc ccactcattc ccgcacgctc tcactgcgca caatgacggc
     6541 gcggagtcga tttgacaatc gagcatcctc atctagactt aattcatgcg cgcgcgaaca
     6601 acatgtccca gaagcggcac attttcaggc atggttgcgg gagtcgtcct ctccctgact
     6661 gccctgcttt cattgcttcc ctctgccgcc tctgcaagtc gaaccctcac agtccccatc
     6721 tccatcgcca cctacactga cgaccaagtc ggcttcccga catttgtgat gaaatgggat
     6781 gaaggaagcc aaccggatcc attgtcgttg cggtgggggc ggagtcaggt tcctgtcaga
     6841 gggaccggga tgagttccat tcagcgggcc tttcgctttg ccgtcgaacg cctggctcca
     6901 tccgtccgtc cgaccggaac cttgtccctc tatgcgagat tttccgggcc attgaacgcg
     6961 gagggagcga ccgcagaagc cgccctggcc atcgggtttc tcgccctgct gaaaggtgac
     7021 cacctgaaac aggacattac gatcaccggt acgctggaag gcgatggccg gatcggacaa
     7081 gtcgcgcagg tggctgaaaa aacgacggcg gcggcgaggg ctggctacca cgtactgctg
     7141 gtgccgcgag ggcaatttta tgccccgcga gtcaatcgag tcagcctgaa cctcgaatcg
     7201 aatgtgttgg tgcgtgaagt ggagacgatc gaggaagcct acgaaatcat gaccgggaaa
     7261 aagctctagc ctttccccct ctcccccagg gccgtgtgcc gtctccggca gcgctctcct
     7321 cactcctgat tcgcgccaat gaacaagcca tcgatacagg atcgatggct tgttatcgtt
     7381 ttgccgagag gcccttgaat cctgccccga tcacatccaa ttgttgatca agaggaaggc
     7441 cgcccagaag ctggggtgcg tgtgaccgcg ctgggaaaga atcttttgct gggcccgttg
     7501 caacgcaacg gcctttgaca gcgcgggatc ctgcaattgc ctgtagaatt ccgacacgag
     7561 ttccgtggtc gtttcatcat ccgacgtcca caaactcgcc aaagccgtcc gcgcccctgt
     7621 cttgacggcc acaccactca acccgagcgc cgcacgatcg tcctcggcag caatctcgca
     7681 ggagctgagc gtcaacaaat ccaagggctg ctgccggtat tgctgaagac ccaccagctg
     7741 cgacaaccgg tccatcgtga tcttgccgtc atgagccagc acaaacgaat ctctggcctc
     7801 ggtgccgacg accgtgtggg acgccacatg aacgatcccc actccctggt ccttgatctc
     7861 ttcctccaga gacggtgcag agaactgctt gttcatcagc agcttcccgc catacagcgt
     7921 tctgatggct tgtacttcgg actccgcgta ccgcggagca gacaggccct cgaccgattc
     7981 agtcaggccc accgacagca gactgccttt tcggcgctgg gcagcactca gatcggtcaa
     8041 cgccagactc ggcgtgacgg ccacggcaag cgaatccacg agaaaatgcc gcccatcatg
     8101 caaggccccc attggaatag tccggagcga cccgtcggcc accatcacga gcgtatgaat
     8161 cccggctccc tgcaaatcct gctgaagagg cgcaaccagc cacccatgca acgtctgcgc
     8221 ggaagacagg tagttctgcg attgggaatc ctggatcagc cgcctgaacg aacgaatttc
     8281 cttcgtcagc ctctctcccg ccacaggaac cctgacctgc ttaagcccat tggcagtttc
     8341 caggagcagt tcgagccggt ccgggaaaga aatcggatac accacggcgg tgccgggcgc
     8401 cagcgtgccg acgcctcgat gactcgcgac cgtcgtcaca caatcgtcct ggaaataatc
     8461 ctgcagctcg gccgcatggg agacctcaac cgtatccttc acctgaacaa gcaactgttc
     8521 attctggtcg ggcgtcttcg ccgccgccgc tctccgcaac aagacatctt catactccac
     8581 aaacagcggc gcaacagact cgtagtacga gtggtgccgt ccctgatacc ccacggaata
     8641 ttcgtagtga atcggcttca gcagcgtcac ggcacgctga tacgcggcca gtgcttcttc
     8701 ttctttgccg tccgcacgca acagcctcgc cgtttgccat tgccaacggt acagcgattc
     8761 cggcgcgttc accttttgag ctgcgaaggt ggccttccgt gaatactcca acgcctcggg
     8821 attgcgatgc tcctgctcca gcaatccacc caaatagccc caggcgtacg attgtccacg
     8881 cgcatcgccg atgcgtccgg ccacttcacc ggccgcgata aaggcatcgg acgattgccg
     8941 taacaggccg gctcccagcc cggcggaagc aaccgcctcc gtcgcgcggc ccttccccgc
     9001 atccgatttc ctggcgacgc ctgatttcgc ggtcgtggcg gcgaacagct cctgatagcc
     9061 aagcccgata ttcaacagac cggccgtctt cgcctgacca tcccccagcg accgcacatc
     9121 cgaccaggct tgatccaaat gacgatgcgc ttcactcagt tgttggttct ggagcaaggc
     9181 catcgcgccg ttgatttggg ccgttgccgc cagtgccggc tgcttggtct caatggcaag
     9241 gctccggctt tccgcataga cgtcgatcgc ttcggcaaac tgccgacggg ccgtcagagc
     9301 attacccaga tcgttcagga ccccggcaac caacagaggc ttgttctcct cacgtgccaa
     9361 cgtcaaggct ctggtcaaat gttcggtggc gggttcgtct ttcccgagca cgtgaaaggt
     9421 gttgccgagc tgagcgagaa tcgtcgccgt gaggccacgc tcaccgttct gttccgagag
     9481 tttgagcgcg gcttgcaacg tgccttgcgc gcgacggatc tgtccttcct gctgaagcgc
     9541 atgcgcgagg ttgatcaacg cctggcactg ctcttttgcc tgtccactct cttcataccc
     9601 gcgcgcagca ttcatccagt gcgcggcggc ctgggcaaac gcaccctgct gaaactgtgt
     9661 cgtcccctgt ctcatttctt gcgaaggcga gatcgacgac gcacccgaac cgccaaataa
     9721 ttccatcgcg atcccgctca caggccaggc caatccgctc aacgcaagca ccccgacgcc
     9781 cagcatcgtc aacggcgagg aaagcgcgcg cgtggcggag ggaccagcgg atccatgcct
     9841 ccgcttcatg ccgcgccgag tgaccatgtt acgcttcgta ggcatgacgt cctccttggg
     9901 g
//
LOCUS       DAOQRM010000090         9884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6179_length_9884_cov_14.968868, whole genome shotgun sequence.
ACCESSION   DAOQRM010000090 DAOQRM010000000
VERSION     DAOQRM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6179_length_9884_cov_14.968868"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /gene="tilS"
                     /locus_tag="PKK81_08415"
     CDS             complement(<1..1336)
                     /gene="tilS"
                     /locus_tag="PKK81_08415"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNV32939.1"
                     /translation="MTGSQSDLHHPLHKQVARAIRARRLLQPGQKVLVAVSGGPDSVA
                     LLSILHQLAPAWNFSLTVVHCNYGLRGAESDGDASFVAALCRRLELPCHIRRLTVDRS
                     GTGESSSLQARARESRYRLFRDVVAELGADRVALGHTADDQAETVLLRMLRGAGLRGL
                     AGMPHIRESLFVRPLLSRSRQEILVYLQAVGLSYRTDSSNAKSIYLRNRVRHELLPVM
                     QSLAPAAARILARQADLLRDDDRLLDALAAHRLERTIRSRDSATIVLDRTVLLKQPPA
                     LQRRILRQAIQAVAPSTGAPRSDVLLSMLASLASHSGGIWNAGAATVACEQDQVRLTA
                     VSPVPPAGGTVLQPIHTAPVPDVVTIAALPSAVSWPLTGAVIRLRAVTRERGLVLLEK
                     PSSSVALLDADRLSWPLTIRPWCHGDWFFPTGMAGRRKKLQDYFTDAKLGRSM"
     gene            complement(1340..2305)
                     /locus_tag="PKK81_08420"
     CDS             complement(1340..2305)
                     /locus_tag="PKK81_08420"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNV32940.1"
                     /translation="MKISRGLTPSTSRPYSVVTIGNFDGHHHGHRALLDRVVATARRE
                     AGTALVLTFDPHPVKILAPQVNLMFLTTPEEKLARFEAAGIDEVVFLEFTTAFAALSP
                     AQFAKQVLCDGIGTRELYVGEHFAFGKGRAGRIADLLELGAQLGFRVHPMAPVTIDGE
                     VVSSTRIRQLIQAGELQKAVRLLGRPYSIEGTVISGAHRGTELGWPTANLRLPEGRVI
                     PPDGVYAAQVSWKGSGLNAVVYIGTRPTFGAGERLLEVSILDERLELYGEVIRVDLLG
                     FIREDRVFASAEALTRQIALDVDAARAQLRDAGITASLSALQPRS"
     gene            complement(2302..3285)
                     /gene="hemB"
                     /locus_tag="PKK81_08425"
     CDS             complement(2302..3285)
                     /gene="hemB"
                     /locus_tag="PKK81_08425"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNV32941.1"
                     /translation="MAFPIQRLRRLRETEPLRRMVRETSLTPNDFIYPVFVTEGQGRR
                     EPIVSMPGQSRLSIDLLVKEAHEVKALGIPAMILFGIPDRKDERGSSGFDPEGIVQRA
                     IRAVKAQVPDLVLITDVCIDEYTSHGHCGIVRDGKILNDETLDCLRTMAKTHAQAGAD
                     MVAPSDMMDGRVAAIRDELDCAGFVDIPIMAYAAKFASCFYAPFRDAANSSPQFGDRQ
                     SYQMDPANKREALREIDLDIEEGADIIMVKPAMPYLDIISAARERTLLPIAAYQVSGE
                     YSMIKAAAQAGWLDERRAMLESLLSIKRAGAEIILTYFAKEAARLLQSRPA"
     gene            complement(3293..3685)
                     /locus_tag="PKK81_08430"
     CDS             complement(3293..3685)
                     /locus_tag="PKK81_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV32942.1"
                     /translation="MVPVKSFMVPKDKFITVERDMDVRSAGRVMRDRNIGSLFVTNGK
                     DVIGILTDTDMVRRVVATGADMTKTTVEQIMSAPLVTIEEHKTLLDANDLMAQTHLRH
                     LGVTKDGQLVGMISVRDLVVFLTNLPRK"
     assembly_gap    3744..3843
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3892..5457)
                     /gene="cobA"
                     /locus_tag="PKK81_08435"
     CDS             complement(3892..5457)
                     /gene="cobA"
                     /locus_tag="PKK81_08435"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNV32943.1"
                     /translation="MTTRAGTVYLVGAGPGDPKLLTIRGKECLEQADVVLYDYLANEA
                     LLQHVSPTAERLYVGRRGRGQYRDQAEINRLLIDHAQAGKRVVRLKGGDPFVFGRGGE
                     EAEAVAAAGLEFEIVPGVTAAVAAPAYAGIPVTHRTMASTVTFVTGHEDPTKDRSGVE
                     WRRLATVHGTLVFLMGMTNLPKIVQCLRAEGKEGTTPVALIRWGTRASQRTVVGSLDD
                     IVEKAAAAHMEPPTVIVVGEVVRLREQLNWFERRPLFGKRILVTRPRPQAAEFSDLIA
                     AQGGEPVECPTIEIVPPEDWAPLDRALARLSTYNWLIFTSVNGVAPFMTRLLETQRDV
                     RALSGLTICCIGPRTAEALAAYGLRADVIPEQFQAEGILEVLAARQMRGARVLIPRAL
                     VARELLPDQLRTQGADVDVVPVYRTIRPAVATDCLMEQLRTGGIDVISFTSSSTVRNF
                     VELFTTKDELLRSVGKATVACIGPITAATAREAGLTVQVMAGQNTVPALADAIVDFVV
                     NPIDGRTASAVIH"
     gene            complement(5463..6395)
                     /gene="hemC"
                     /locus_tag="PKK81_08440"
     CDS             complement(5463..6395)
                     /gene="hemC"
                     /locus_tag="PKK81_08440"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNV32944.1"
                     /translation="MSRSTLILGTRGSKLAVHQSQWVQARLQELAPGLTISLQRIQTS
                     GDKILDVPLAKIGGKGLFVKEIEDALLSKEIDLAVHSMKDVPTALPEGLDILCVPPRE
                     DPRDALITRDGCRLDQLKPGARIGTSSLRRQAQLLHHRPDFTIEMLRGNLDTRLRKLR
                     EGQFDAIVLAAAGLRRLAWDAEITEYLPVHLSLPAIAQGALGIEARSDDTFVRELLSR
                     FEHRPTRITVTAERALLHRLEGGCQVPIAAHAELDGDRLTVDGLVASVDGRRVIRHQI
                     QGPASEAQALGTKLAERLLADGGDVILKEIYGQA"
     gene            complement(6481..7887)
                     /gene="hemA"
                     /locus_tag="PKK81_08445"
     CDS             complement(6481..7887)
                     /gene="hemA"
                     /locus_tag="PKK81_08445"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNV32945.1"
                     /translation="MHVIVVGLSHKTAPVEIREKLAVPESRLREALSRLCSYPGVREG
                     LLLSTCNRVEVYAVVEELETGYGRVQEFLADTHLSLSSEHLTPHLYWHAGDRAIGHLF
                     RVASSLDSMIVGESQILGQIKDAFEVALTHKASGLLLNKIVKKAISVAKRVRTETKIA
                     ETAVSVSYAAVELAKKIFSNLTEKTVLLIGAGEMAKLAARHLIANGVRQVRITTRNFQ
                     HGLELAQRFNGTAVPFEDYKTELAGADIVLVSTGASHYLVTADDVQRAMRQRMSRPMF
                     LIDISVPRNIDPAVRPIDDAFLFDIDDLHMRVEQNREDRLREAAKAEQMVVEEVAVLG
                     QWLQSLEVTPTIVALRSRTEELKRREVDKILARLAHLSSEDRAAVEALASAIVNKMVH
                     GTMVTLKAEAKSSSGAAYVDAARRFFNLEETPAVYPPGQADVPELRGENIAGNGVGRL
                     PEASLVQHTSKEQGRRVS"
     gene            complement(7889..8695)
                     /gene="ccsA"
                     /locus_tag="PKK81_08450"
     CDS             complement(7889..8695)
                     /gene="ccsA"
                     /locus_tag="PKK81_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNV32946.1"
                     /translation="MSSVFFMLTMGLYFVSTVCYLAYLLRRAETLSKVSLGITAVGFA
                     THTVALVARMTDTAQAQLPTFHEALSFFSWMLILVFLAVEFRRQLHVLGSFIVPLALV
                     SLVTAAAFRSDEASSLQPVFKTLWVHVTLSMLGTVGFAVAFVAGVMYLIQDGLLKSKR
                     FNVLYAKLPALDYLDHLNQQSIVMGFPLLTLGIISGAFSAEIVRGTYVNWNPEQTWAM
                     VTWGFYFAVLVGRLTIGWRAKRAAYLTIIGFAGVILTLIGVVLKSHGSVS"
     gene            8760..9512
                     /locus_tag="PKK81_08455"
     CDS             8760..9512
                     /locus_tag="PKK81_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV32947.1"
                     /translation="MGRERSQGKRAQSAPPAGTLFIVGVPIGHPDDLTIRGLATLRQV
                     DLIAAKNPQATHALLAHHGIHTTITTYDRNNAADKVPLLLERLRQGCRVALVSDCGMP
                     VVYDPGRLLITAASRAQIPVKVIPGTSAVVAATALAGMDGNAFVFDGRWTGGTKALTG
                     RLQLLQSEPRTMIFFPPAQSLRQFLTLLRRTLGNRKVVMAIDMTRSTEQIIRGRVQQL
                     LSNKPSPENASHVALVVEGARLRRKNSVKQSR"
     gene            complement(9513..>9884)
                     /locus_tag="PKK81_08460"
     CDS             complement(9513..>9884)
                     /locus_tag="PKK81_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNV32948.1"
                     /translation="ASEEAPRFSFVRTISCSPVATQRFGWYHLSSPQLRIAMSEVPSN
                     IHVPDEELDLRGVICPYNFVKTKLKLESMAPGQLLLVHLDDGDPIRNVPRSVSDDGHD
                     VVSQERADQSYRVVIRKRFDE"
BASE COUNT         2089 a         3114 c         2775 g         1806 t
ORIGIN      
        1 gcatagaccg acccagtttt gcatctgtga agtaatcttg caatttcttt cgtcgccctg
       61 ccatgccggt cggaaaaaac caatccccgt ggcaccaggg gcgaatcgtc agcggccacg
      121 acaatcggtc ggcatccagc agagccacgg aagacgatgg cttctccagg aggaccaatc
      181 cccgctcgcg cgtcaccgcg cgcagacgaa tcaccgctcc cgtcaatggc caggaaacgg
      241 ccgaagggag ggcggctatc gtcacaacat cggggactgg agccgtatgg atcggttgaa
      301 gaactgtccc acccgccgga ggcactgggg agaccgccgt caacctcacc tgatcctgtt
      361 cgcaggccac cgtcgcagca cccgcgttcc agatgcctcc cgaatgtgag gccagtgatg
      421 ccaacatcga cagcaggaca tcgcttcgcg gcgcgccggt tgacggcgcc accgcctgga
      481 tcgcctgacg gagaatccgg cgctgtaggg ccggcggctg tttgagcaac actgtacgat
      541 cgagcacgat cgtagccgaa tcgcggctcc gaatggtccg ctcgagacgg tgtgcggcca
      601 gcgcatccag cagccgatca tcgtcccgta ggagatcagc ctggcgcgcc aggatgcggg
      661 ctgcagccgg ggctaacgac tgcatcaccg ggagcaattc gtgccggaca cgattgcgaa
      721 gatagatcga tttggcgttg ctggagtcgg tccgatagga caatcccacg gcctggagat
      781 agaccaggat ctcctggcgc gaacgactga ggagcggccg aacgaatagg ctctcccgga
      841 tatgcggcat gccggccaat cctcgcaaac cggccccgcg caacatccgc aagagcacgg
      901 tttcggcctg gtcatccgcc gtatgcccga gcgccacacg atctgcgccg agttcggcga
      961 ccacgtcacg aaacagccga tagcgagact cccgggctcg cgcctgcaat gagctcgact
     1021 cccctgttcc actgcggtct accgtgagcc gtctgatatg acacggaagt tcgaggcgac
     1081 ggcagagtgc cgcgacaaac gaggcatccc cgtcggattc ggcgccccgc aacccgtagt
     1141 tgcaatggac gacggtcagc gaaaaattcc aggccggtgc gagctggtgc agaatggaga
     1201 gcagtgcaac cgagtctggc cccccggaca cggccacaag gaccttctgc cccggttgca
     1261 ggagcctccg cgcacgaata gcccgggcga cctgcttatg aagcgggtga tggagatctg
     1321 actgggaacc tgtcatggct catgaccggg gctggagggc cgagagcgac gcggtgatcc
     1381 ctgcatcgcg caactgtgca cgggccgcat ccacatccaa cgcaatttgc cgggtcaggg
     1441 cttccgctga ggcaaagacg cgatcctcac gaatgaaacc gagcaggtct actcgaatca
     1501 cttctccata caattcgagt cgctcatcga ggatcgagac ttccaggagt cgctcgccgg
     1561 cgccgaaggt cggcctggtc ccgatgtaga ccaccgcatt caagccactc cccttccagg
     1621 agacctgtgc cgcatacacg ccatccggcg gaatgacacg cccctcaggg aggcggagat
     1681 tcgccgtcgg ccaaccgagt tccgttccgc gatgggcgcc tgaaatcacg gtcccttcga
     1741 tgctgtaggg acgtcccagc aggcgaacgg ccttctgcag ctcccccgcc tggatcagct
     1801 gacgaatacg cgtggagctc acgacctcgc cgtcgatggt cacaggcgcc atcggatgaa
     1861 cgcgaaaccc gagctgggcg ccgagctcga gcagatccgc aatgcgtccg gcacggccct
     1921 ttccgaaggc aaaatgttcc ccgacataca actcccgcgt accgatcccg tcgcacaaga
     1981 cctgcttcgc aaactgagcc gggctcagcg cggcaaaggc cgtcgtgaat tcaagaaaga
     2041 cgacctcatc gataccggcg gcctcgaacc gggccaactt ctcttccggt gtcgtcagaa
     2101 acatgagatt gacctgcggg gccaggatct tgacgggatg gggatcgaat gtcaggacga
     2161 gcgccgtccc ggcctcgcga cgggctgtcg ccaccacccg gtcgaggaga gcccggtgac
     2221 catgatggtg accgtcaaaa tttccgatgg tcacaaccga atagggtctg gacgtggacg
     2281 gggtgagacc gcgggaaatc ttcatgcggg ccgtgactgg agaagcctgg cggcctcttt
     2341 ggcaaaatag gtcaagatga tctcggcgcc cgctcgcttg atcgagagca gcgactccag
     2401 catcgcgcgg cgctcatcca accagccggc ttgtgccgcc gctttgatca tgctgtattc
     2461 cccgctcacc tggtaggcgg caatcggcag aagcgtccgt tcgcgcgcgg cgctgatgat
     2521 gtccaaatac ggcatggcgg gcttcaccat gatgatgtcg gccccctcct cgatatccag
     2581 gtcaatctcc cgcaaggctt cccgcttgtt cgccggatcc atctgatagg actggcgatc
     2641 gccgaattgc ggactggaat tggcggcatc acgaaacggt gcgtaaaaac aggacgcgaa
     2701 cttggccgca taggccatga tgggtatatc gacaaatccg gcgcaatcca actcatcgcg
     2761 aatcgcggcc acgcggccgt ccatcatgtc ggacggcgcg accatgtcgg cccccgcctg
     2821 cgcatgggtc ttcgccatgg tcctcagaca atccagcgtc tcgtcgttga gaattttgcc
     2881 gtcccgcacg atgccgcaat ggccgtgact cgtgtactca tcgatgcaga catcggtgat
     2941 cagcaccaga tccggcacct gcgccttcac tgcacgaatg gcgcgttgca cgattccctc
     3001 ggggtcgaac ccggaactgc cgcgctcgtc ctttcgatcg ggaatcccga agaggatcat
     3061 ggccggaata cccaaggcct tgacctcgtg cgcttccttg accagcagat cgatcgacaa
     3121 tcgcgattgt cccggcatcg agacgatcgg ttcacgacgt ccctgccctt cggtgacgaa
     3181 cacgggatag atgaaatcgt tcggcgtcag gctcgtttct cgaaccatcc gccgaagcgg
     3241 ctccgtctcc cgcagacgac gcaggcgctg aatcggaaat gccatggtcc gctcacttcc
     3301 tcggcaagtt ggtcaggaac accaccagat cgcgcaccga aatcatgccg accagctgcc
     3361 cgtccttcgt cacgccgagg tggcgaagat gggtttgcgc catcaagtcg ttcgcgtcca
     3421 gcagcgtctt atgttcttcg atcgtcacga ggggggcgga catgatctgc tcaacggtcg
     3481 tcttggtcat gtcggccccg gtggcgacca cccgccgtac catgtccgtg tctgtcagaa
     3541 tgccgatgac atcttttccg ttcgtgacga acagactgcc gatgttgcga tcgcgcatga
     3601 cccgccctgc agagcgcaca tccatgtcac gttcaaccgt gatgaacttg tctttcggaa
     3661 ccatgaatga cttgactgga accatcagaa tcctcccctc ttggtaagaa acatcttcac
     3721 gatcactgcc atccggtcaa tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnaatcctc ccctcttggt aagaaacatc ttcacgatca ctgccatccg gtcaatggat
     3901 caccgcggaa gcggttcggc catcaatcgg attcacaaca aaatccacga tggcgtcggc
     3961 aagcgctgga acggtgttct gtccggccat cacctgaacg gtgagccccg cctcgcgagc
     4021 ggtcgccgca gtaatgggtc cgatgcaggc cacggtcgct ttcccgaccg agcgcaacaa
     4081 ctcgtccttc gtcgtaaaca actcgacgaa gttcctcacc gtcgaggaac tcgtgaaaga
     4141 aatcacgtca atccctccgg tacgaagctg ctccatgagg caatccgtcg cgactgcagg
     4201 cctgatcgtg cgatacaccg gcaccacatc cacgtccgcc ccctgggtac gtagctgatc
     4261 cggaagcagc tcccgggcca ccaatgcacg cggaatcaac acgcgagccc cgcgcatctg
     4321 ccgggcggcc aacacctcca ggattccctc ggcttgaaac tgttccggaa tcacgtcggc
     4381 acgcaagcca taggcggcca gagcctcagc ggtgcgcggt ccgatacaac aaatggtgag
     4441 gccggacaag gcgcgcacat cacgctgcgt ctccaacagt ctggtcatga atggagcgac
     4501 cccgttcaca ctggtgaaga tcaaccagtt gtaggtcgac agacgagcca gggcacgatc
     4561 cagcggagcc cagtcctcgg ggggcacgat ctcgatggtc ggacattcca ccggttcccc
     4621 gccctgagcc gcgatcaaat cggaaaactc cgccgcttga ggcctcggcc tcgtcaccag
     4681 aatccgtttt ccgaacaagg gccggcgctc gaaccaattg agttgctccc gaagacgcac
     4741 cacctcgccg accacgatga ccgtgggcgg ttccatatga gcggccgccg ccttctccac
     4801 aatatcgtcc aagctcccca caacggttcg ttgcgaagct cgcgtccccc accggatgag
     4861 ggcgacgggt gtggtgccct ctttaccttc ggctcgaagg cattggacga ttttcggcag
     4921 gttggtcatg cccatgagaa acaccagcgt gccgtgtacg gtcgccaggc gccgccattc
     4981 gaccccactt cgatctttcg tggggtcttc gtgtccggtg acgaaggtca cggtagacgc
     5041 catcgtgcga tgggtcaccg gtatacccgc atacgccggc gccgcgacgg cggccgtcac
     5101 accaggaacg atttcaaatt ccagcccggc agcggcaacg gcttccgcct cttccccgcc
     5161 ccgaccaaag acgaagggat cgccgccttt caagcggacc acccgcttcc ccgcttgagc
     5221 gtgatcgatc aacaaccgat tgatctccgc ctgatcgcga tactgcccgc gcccgcgccg
     5281 gccgacgtac agacgttcag ccgtcggaga cacatgctgc aagagcgctt cattcgcgag
     5341 atagtcgtac agcaccacat cggcctgctc cagacattcc ttgccgcgta tcgtgagcaa
     5401 cttaggatct ccgggaccgg ccccgaccaa atatactgtt cctgctcgcg tcgtcatacc
     5461 actcaggcct gcccgtaaat ctccttgagg atcacatcac ccccatcggc caacaaacgc
     5521 tccgcaagct tcgtccccag agcctgggct tcactcgccg gaccctggat ttgatgacgg
     5581 atcacccgac ggccgtcgac gctggctacc aggccatcga cggtaagacg atctccgtca
     5641 agctcggcat gcgccgcgat cggcacttgg catccgcctt cgaggcgatg caacagcgca
     5701 cgttctgccg tgacggtgat ccgggtgggc cgatgctcaa accggctcaa cagttcgcgc
     5761 acaaaggtgt cgtcgctgcg ggcttcgatg ccgagggcac cttgcgcaat agccggcaaa
     5821 ctaagatgca cgggaagata ttcggtaatt tccgcatccc aggcgagccg ccgcaatcct
     5881 gccgcggcca agacaatcgc gtcgaattgc ccctcacgga gtttcctcag gcgtgtatcc
     5941 agattgccgc gcaacatctc gatggtgaaa tcagggcggt gatgcagcaa ctgggcttgc
     6001 cggcgcagac tgctggtgcc gattctggcg ccgggcttca attggtccag ccgacaaccg
     6061 tcgcgcgtaa tcaacgcatc ccgcggatct tctcgcggcg gcacgcacag aatgtccaac
     6121 ccctccggca gagccgtcgg cacgtctttc atgctgtgca cggccaggtc gatctctttg
     6181 ctcaacagcg cgtcttcgat ttcctttaca aacagtcctt tgccgccgat ttttgccagc
     6241 ggaacgtcga ggatcttatc gcccgatgtt tggatgcgct gcagcgagat ggtcaggcca
     6301 ggcgcgagtt cctggagacg ggcctgcacc cactggcttt gatgcacggc cagcttgctg
     6361 ccgcgcgtac caaggataag tgttgaacga ctcatgagtg accgtcactc gctcgagcgg
     6421 ccacggatac ccgatcacat gtggaaacga atctgaccat gaaatttctc agcggcggtg
     6481 ttagctcacc cgccggcctt gctccttgct cgtgtgctgg accagcgacg cctcgggcag
     6541 gcgaccgact ccgttcccgg cgatgttctc gccccgaagc tccgggacgt cggcttgtcc
     6601 gggcggatac acagcgggtg tctcttccag attgaaaaac cggcgggccg catcgacata
     6661 cgccgcgccg ctcgaggact tggcctcagc tttcaacgtc accattgtgc catgaaccat
     6721 cttattgacg atggcggacg ccaaggcttc caccgcggct cgatcctcgg acgacagatg
     6781 cgccaaccgc gccagaatct tatcgacttc ccgccgtttg agttcttcgg tccggctgcg
     6841 aagtgccacg atcgtcggcg tcacctccag tgactgcagc cactgcccaa gcaccgcgac
     6901 ttcttcaacg accatttgct cggccttcgc cgcttcccgc agacggtctt ctcggttttg
     6961 ctccactcgc atgtgcaggt cgtcgatatc gaacaggaac gcatcatcga tgggccggac
     7021 agccgggtcg atattgcgcg gcacggaaat atcaatcaag aacatgggac gactcatccg
     7081 ctgtctcatt gcgcgctgca catcgtcggc cgtcaccagg taatgcgagg ccccggtgga
     7141 caccagcacg atgtcggccc cggccagttc cgtcttgtag tcctcaaacg gcacggctgt
     7201 accgttgaac cgctgagcca attccaaccc gtgctggaaa ttacgggtgg taatgcgcac
     7261 ctggcgcacg ccgttggcga tcagatgccg cgccgccagc ttggccattt ccccggcccc
     7321 gatcagcaac acggtcttct cggtcaggtt tgaaaagatc ttcttggcca actccaccgc
     7381 cgcatagctg accgacacag ccgtctcggc aatcttggtt tcggtgcgca cgcgtttggc
     7441 caccgaaata gccttcttga cgatcttatt cagcaacagg ccggaggctt tatgcgtcaa
     7501 cgcgacttcg aacgcatcct tgatttgtcc gaggatctga gattcaccga caatcatcga
     7561 atcgaggctg gaggccacac ggaacagatg accgatggcg cgatcgccgg catgccaata
     7621 gaggtgaggc gtcagatgtt cggaggaaag ggagagatgg gtgtcggcca gaaattcctg
     7681 cacgcgacca tacccggttt ccaactcttc gacgaccgcg tagacttcca cccggttgca
     7741 cgtcgaaagt aacagccctt cgcgcacacc gggataagaa cagagacgac tgagggcttc
     7801 cctgagccgg ctttcaggca cggcgagttt ctcgcgaatc tcgaccgggg cagttttatg
     7861 gctgagccca acaacgatga catgcatgtc aggacaccga cccgtgactt ttcaacacca
     7921 caccgatcag ggtcagaatc acgccggcga acccgatgat cgtcagatac gcggctcgtt
     7981 tggcacgcca tccgatggtc aaacgtccga ccaggacggc aaagtagaag ccccaggtca
     8041 ccatggccca cgtctgttcc gggttccagt tcacataggt gccacggacg atctccgccg
     8101 agaaggcgcc gctgatgatc ccgagcgtca ggagcggaaa ccccatcaca attgactgct
     8161 gattcaagtg atcgagataa tccagcgcgg gcagttttgc gtagagcacg ttgaagcgct
     8221 tcgacttgag aagaccatcc tgaatgagat acatcacccc ggcgacgaat gcgacggcaa
     8281 agccgaccgt gcccaacatg ctgagggtga cgtgaaccca caacgttttg aagaccggct
     8341 gaagactgga cgcctcgtcg gagcggaacg ccgcggcggt gaccaacgac acgagcgcca
     8401 gtggtacgat gaaggaacca aggacatgca actggcggcg aaattcgacg gcgagaaaca
     8461 cgagaatcag catccaggag aaaaacgaca gggcttcgtg aaatgtcggt agctgcgcct
     8521 gggccgtgtc cgtcatccgg gcgaccaagg ccaccgtgtg ggtggcaaac ccgacagccg
     8581 tgattccgag ggacaccttc gacagggttt ccgcccgccg gagcagatag gccagatagc
     8641 aaaccgtgct cacgaaatac aggcccatcg tgagcatgaa aaacaccgaa gacattgtgc
     8701 tctatcctcg caacatttca gcgcaaagcc actttttccg gtaactggaa attatatcga
     8761 tgggacgaga acggagtcaa ggaaagcgcg cgcaatctgc ccccccggca ggaacgttat
     8821 tcatcgtcgg cgtgccgatc ggacatcctg acgatctgac gatccgaggt ctcgcgactc
     8881 ttcggcaggt tgatctcatt gccgctaaaa atccgcaggc cacgcacgcc ctcctggccc
     8941 accacggaat ccacaccacg atcacgacct atgatcggaa caatgccgcc gacaaagttc
     9001 cactattgtt ggaacgcttg cgacagggat gtcgagtggc gctggtgtcg gattgcggaa
     9061 tgccggttgt ctacgatccg ggtcgattgc tcatcacagc cgcatcgagg gcgcagattc
     9121 ccgtcaaggt catcccggga acttccgctg tggtggcggc aacagccctc gccggaatgg
     9181 acggcaatgc cttcgtcttt gacgggcgtt ggaccggtgg cactaaagca ctcacgggcc
     9241 gtcttcaatt attacaatcc gagccacgca cgatgatttt cttcccaccg gcacagagcc
     9301 tccgacaatt tctcactctg ctacgacgga cgctaggcaa tcggaaagtc gtgatggcca
     9361 tcgacatgac tcggagtacg gagcagatca tacgaggacg agttcagcaa ctgctctcga
     9421 acaagccgag ccccgaaaac gcgtcacacg tcgctctagt ggtagaaggc gccagattga
     9481 ggaggaagaa cagcgtgaag cagtcgcgat aactactcgt cgaagcgctt acggatcaca
     9541 acccggtagg actgatcggc acgttcctgc gacaccacat catgcccgtc gtcgctcaca
     9601 ctgcggggca catttcgaat cggatcacca tcgtcgagat gaactaacag cagttgccca
     9661 ggagccatgc tctcaagctt gagtttcgtc tttacgaaat tgtaggggca gatgacaccg
     9721 cgaagatcca gctcttcatc gggtacatgt atgttagacg gcacttcaga catggcaatc
     9781 ctcaattgag gtgatgacag atggtaccaa ccgaaccgtt gcgttgcaac gggcgagcat
     9841 gaaatcgtgc gaacaaaaga aaagcggggt gcctcttccg aagc
//
LOCUS       DAOQRM010000091         9849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6212_length_9849_cov_16.818256, whole genome shotgun sequence.
ACCESSION   DAOQRM010000091 DAOQRM010000000
VERSION     DAOQRM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6212_length_9849_cov_16.818256"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PKK81_08465"
     CDS             complement(<1..659)
                     /locus_tag="PKK81_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNV32949.1"
                     /translation="MSDLLSNLPIARKLFLASVIPAFTVLLLSVLTYRSVTTFSDDEG
                     QLNDIYYSQRLASEYLRLVVDLETGFRGFVLTSQEHYLFPYRTAQDHVLNIGRTLEAQ
                     VSAYDDQRALITSVQQLVRQFIDEKEILIDAVKAEHPDEARLYIEEGKGRTLMLKIRQ
                     DMGRFEHLAQTALNTKLAKLAQDRDTMLMTILGGGLFALICMVAALHLIAHSITTPLE
                     RL"
     gene            1009..1950
                     /locus_tag="PKK81_08470"
     CDS             1009..1950
                     /locus_tag="PKK81_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNV32950.1"
                     /translation="MTVPRIARRVMAGEPKKEAAMDYVKPYGVVDSMLAGGALKLSLP
                     PRQLVLRGMLAGAYLGIGTSMAVTVAVETGYWILGSLLFPFGLALAILLGAEIITGSF
                     ALLPCAAVAGQKNAGLRKVFANWGWVFLGNLLGSTLYAGLFAIALTTAGDAQINAVGT
                     KLIAIAEAKTNYYASHGTAGLLAAFTKGMLCNWMVSLAVVAAFMSTSFSGKILAIWGP
                     TLLFFSQGFEHAVVNMFLMPVGMLLGANVSVSTWWLWNQIPVTVGNLVGGMLFTGLAI
                     YGAHHTSAPAATPSVPATMPAQPGTARADQPGYSPSY"
     gene            1967..3565
                     /locus_tag="PKK81_08475"
     CDS             1967..3565
                     /locus_tag="PKK81_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNV32951.1"
                     /translation="MAVVYLRRLHGWRFVLFNAVLGLSHIVVLFNAGSYIALLPHVSG
                     DLGGVLPSFLTWAQTDFMVGLALGFPLARYLSGRIGDYRLLIGAFMVYSVASYLCGDS
                     QTLAQFVPARIVQGIAGGITLPIAQSMLLNEYPKRLKAVALTVWGLFSITPFTIGMPV
                     GGYIAYMLGWRFLFYLDFVLTLVIVGTLAALLYGRGFRRRYARFDLVGFVLLAVLLLG
                     LQTLLNMGNDFDWLDSPFLQAIAVVLLVAFPCFVIWELGERHPTLDLRLFMHRNFVIG
                     VISLTLGFFSIQGLLSLLIVQIQLILGYSSKLAGLALLPLVLLGAPAIAVMHILCKYI
                     DARWLICLNSLGFAWTFYWLGLYDDPHSYEELFWPMLVEGIFLGSFFTPVTVLTLHGL
                     SGPLIMRAAEVANLLRVAAGAFGITFQGIVLFRRIHFHQLHLADHFGGRQSISFDPMG
                     QLTAKLSAAGLSQAQIQAKLGLLIRQEAAILGLNDAFLVASVLFVGLGILVWFAHPTH
                     LPVAPAPADELREMRAEEMMEEVP"
     gene            3592..>4323
                     /locus_tag="PKK81_08480"
     CDS             3592..>4323
                     /locus_tag="PKK81_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV32952.1"
                     /translation="MQIVLGIGCALFFGGCAWIPKGDQPAQYLEAPEMTETLAEVTSR
                     LQNWPEDRWWEQFGNPELNGLIEQSLTDNPGLKHAAARLRQAMSLVKVEGARLLPFLE
                     ADASLTYERISQHGVFAALNPEVAGIRIMYGIINPLSFRYEFDFWGKNRAMLEAALGH
                     AAAEEAETAEVRLRLTTGIARAYFRGQALQQQLTIVKTIVGIRRELKTLAETRFRLGL
                     DNDQPVKIAVADYEAAFKRQAAIRDQ"
     assembly_gap    4324..4423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4424..5225
                     /locus_tag="PKK81_08485"
     CDS             <4424..5225
                     /locus_tag="PKK81_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV32953.1"
                     /translation="AVADYEAAFKRQAAIRDQLDVQRHLLARLVGKGPDEGAHLFAKP
                     KVIVPSQIAAPDHLSLGLLVHRPDLAAALYRAHAASRLVRVATTQFYPTIDLTGFVGF
                     NALTLAKGTDKLANFLFSGQSFTYGLAPGLRMPWFEGGRLRGELGAQRAEYDAAVELY
                     NDTLLEAMREVADSLSAWQTTNEIMASHKRLVASLGDDWRLAKVRLVNGLDDDREVLR
                     HRYPVLEQEYALRALESDQLVAAVDLIESLGGGYHNPDIEKRPKQNPS"
     gene            5230..6777
                     /locus_tag="PKK81_08490"
     CDS             5230..6777
                     /locus_tag="PKK81_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNV32954.1"
                     /translation="MTTTTADMSQHPQSAGGSPPAGPYRIHPKAIRARRNRRLLVVAG
                     LVLVATVAYLVHWYTHDRFWVRTDNAYVTGNLVPVAAQASGIVTQVLAEETQFVNRGD
                     LMIKLDEHQAYAALGRARGRLGEEVRRIASLFINRKQLAEKLRSRRARLELAEHDMDR
                     YQRAAPSGAISKQILQNTADKIASLDAEVRETQAELDSLDAQIGGTTVMAHPAVDLAK
                     HQLIEAHLEYTRQQIRAPISGYVAKRKAQVGDRVQPGALLMTIVPLDHLWVEANLRET
                     ELQHVRPGQPALVNVSLYGSKQTFHGTVEGLVPGSGSAFALLPPDNSTGNFIHIVERV
                     PVRIALPADELREHPIRPGLSTVTSINITESGQSVWTSLATPSTAEYETDVYADELPM
                     AESLAKEVIATNLVVGDRGVSIDSLLDEEEEIQRIVPRKGDARTSPEGRVPVDRDRMR
                     THRDPVPSSFIPRRSPDLGATTDPLTPSIGPGSGSLGPEAGRSPSRLRSGFEMGEGRQ
                     VLGTGRH"
     gene            7063..8583
                     /locus_tag="PKK81_08495"
     CDS             7063..8583
                     /locus_tag="PKK81_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV32955.1"
                     /translation="MHNAALNYRTLLSVTNVLNSQRNRQSLFRAVTDQLAKVVRWERA
                     GITVYDLKSDAFRFYAVETNLPKVVLRSDAMIPRAHSAVGWVYDHHQVHVRPHLQRER
                     LFIEDESYLQEGLGRMINLPMVVQEACLGTLNIGSVEAGHPDPADVDFLQQVATQIGF
                     AIAHVNAYEEINRLKEQLARENVYLAEELKANQDFGIVVGRSQAFEQVLTLARQAAPT
                     GATVMVTGETGTGKEVLARAIHERSLRRDRAFVRLNCAALPSGLIESELFGHERGAFT
                     GAEHQRVGRFELADGGTLFLDEVGEMPLEAQAKLLRVLQDGLVDRVGGANAIPVDVRV
                     IAATNADLHRAIQQGRFRSDLYYRLNVFPIHMPPLRERPEDIPILARHFLRVHSQRLK
                     RHCEDFDGPSMERLVQYAWPGNVRELENIVERALILCHERLLRIDPAMVNVRVPPEAG
                     PRRTLHDEERHHILQTLTLLDWRIEGPGGAADQLGLAPSTLRSRMQKLAIRRPSRM"
     gene            9039..>9849
                     /locus_tag="PKK81_08500"
     CDS             9039..>9849
                     /locus_tag="PKK81_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNV32956.1"
                     /translation="MMQLSRRQFLKVSAGTVAVAAVADKALALTALQPVVEVDNPLGE
                     YPDRSWERVYHDQYRYDSSFTWCCSPNDTHACRIRAFVRNGVVMRVEQNYDHQTYEDL
                     YGNRGTFAHNPRMCLKGFTFHRRVYGPYRLKGPLMRKGWKQWMDDGSPELTSDAKRKY
                     KFDSRFLDDMVRVSWDTAFTYVAKGLIVIGTRYSGEAGARRLREQGYAPEMIEMMKGA
                     GVRTFKHRAGMPILGMMGKHANTRFNNCVLPLLDSWIRKVNPDQAQGGRYWN"
BASE COUNT         1805 a         2719 c         3098 g         2127 t
ORIGIN      
        1 aggcgctcca gcggcgtcgt gatggagtgg gcgatgaggt gcaaggctgc caccatgcag
       61 atcaacgcga agaggccgcc gccgaggatg gtcatgagca tggtgtcgcg atcctgggcg
      121 agtttcgcga gcttggtatt cagcgccgtc tgggcgagat gttcgaagcg ccccatgtcc
      181 tggcgaatct tcaacatcag cgtccgtcct ttaccctcct cgatgtagag ccgcgcttcg
      241 tcgggatgct ccgccttcac ggcgtcgatc aagatttcct tttcatcgat gaactgccgg
      301 acgagttgtt gcacggaggt gatcagcgcg cgttgatcgt cataggcgga gacctgcgct
      361 tccagcgtcc tgccgatgtt cagcacatgg tcctgggcgg ttcgataggg gaacagataa
      421 tgttcctggc tcgtgagcac gaaaccgcgg aaccctgttt cgaggtccac gacaagccga
      481 aggtactccg aggccaatcg ctgggagtag tagatgtcat tgagctggcc ttcgtcgtcg
      541 gagaacgtcg tgacgctgcg gtaggtcaag acgctgagga gcagaacagt gaacgcagga
      601 atcaccgacg caagaaacaa cttgcgggca atgggcagat tgctgagcag gtcactcaag
      661 ggcggtcttc atctccgacg atatcgtagt ctgtgcgctt gtctgtgtgc aactttgccg
      721 cctgatccgt cctgtcacaa aaagcgcctc tccggagaaa tcctcttgcc ttcagcgggt
      781 gccggtctct attccgcatg acgttcctgc ggcgggatgc gaaatgagcg gtttcgcacc
      841 gtgcgcctgt gcgatctcgc ccgtattcat gaagttcctc aaggggcgga tgatccggat
      901 ccggtccagg cagcgacgaa cgatcttccc catttgtctc agagcggtac ggaggggtga
      961 aaaacgctaa ggcacgcgcc ttgctcaacg tgtgacagaa ggatctccgt gaccgtgcca
     1021 cgcatcgcgc gacgagtgat ggccggcgaa ccgaagaagg aggctgcgat ggactatgtg
     1081 aagccatacg gcgtggtgga cagtatgctg gccggcggcg cgctgaaatt gagtttgccg
     1141 ccgcgtcagt tggtgcttcg cggcatgctg gccggcgcct acctgggaat cggaacgagc
     1201 atggcggtga cggtcgcagt cgaaaccgga tactggattc tcggcagtct gctctttccc
     1261 ttcggattgg cgctggcgat tctgcttggc gccgagatca tcacgggcag tttcgcgctc
     1321 ttgccctgcg ctgccgtggc cggccagaag aatgccggtt tgcgaaaggt ctttgcgaac
     1381 tggggctggg tttttctggg caatctgctg ggcagtaccc tatacgcagg cctgtttgcc
     1441 atcgctctga ccaccgcagg agatgcgcaa atcaatgcgg tcgggaccaa actcattgcg
     1501 atcgccgaag cgaaaaccaa ctattacgcg tcacatggca cggccgggct cctcgcggcg
     1561 ttcaccaaag ggatgctctg taactggatg gtaagcctgg cggtggtggc ggcgttcatg
     1621 tcgacgtcat tctccgggaa aatcctcgcg atctgggggc cgaccttgtt gttcttttcg
     1681 caaggattcg agcatgcggt cgtgaacatg tttctcatgc ccgtcggcat gttgctgggg
     1741 gccaatgtca gtgtgtcgac ctggtggctc tggaatcaga tcccggtgac cgtagggaat
     1801 ctggtcgggg gcatgctgtt tacgggattg gcgatctatg gggcgcacca tacgagtgcg
     1861 ccggccgcga ccccatccgt tcccgcaacg atgccggctc aaccggggac tgcccgggcc
     1921 gatcagccgg gctattctcc ttcgtattaa gtcatggagt gcggcgatgg cggttgtcta
     1981 tctcaggcga ttgcatggat ggcgcttcgt gctgttcaac gccgtgctcg gcctgtcgca
     2041 catcgtcgtg ttgttcaacg ccggttccta cattgccctg ctgccgcatg tgtcgggcga
     2101 tctgggtggc gtgttaccga gttttctcac ctgggcgcaa accgacttca tggtcgggtt
     2161 ggccctgggc tttcccctcg cgcgatacct ctcgggacgt atcggcgatt accgcctgct
     2221 gatcggagcc tttatggtgt acagcgtggc gtcgtatttg tgcggtgata gccagacgct
     2281 cgcgcaattc gttcccgctc gcattgtgca agggattgcc ggtggcatca cgctgccgat
     2341 cgcccagtcc atgctgctca acgagtatcc caagcgattg aaggcggtcg ccttgaccgt
     2401 ctggggcctg ttcagcatta cgcccttcac catcgggatg ccggtggggg ggtatatcgc
     2461 gtacatgctc gggtggcgtt ttctgttcta cctggatttc gtgctgacac tcgtgattgt
     2521 cggcacgctg gccgccctgt tgtacgggcg aggctttcgt cgccggtacg cgcgcttcga
     2581 cctggtcggg tttgtgctgc tggcggtgtt gctcctgggg cttcagacgt tgctcaacat
     2641 gggcaacgat ttcgattggc tggattcgcc gttcctccag gccatcgcgg tggtgctgct
     2701 cgtggcgttt ccctgtttcg tcatctggga actcggggag cgacatccga ccctggatct
     2761 tcgattgttc atgcaccgca attttgtgat cggcgtcatc agcctgacgc tcgggttctt
     2821 ctcgatccaa gggctgctgt cgctgttgat cgtgcagatc caactcattc tcggttattc
     2881 gtccaagctg gccgggctgg ccctgttgcc gctggtcttg ctgggggcgc ctgccattgc
     2941 ggtcatgcat attctctgca agtacatcga tgcccgctgg ttgatctgtc tgaacagtct
     3001 gggctttgcc tggaccttct actggctcgg cctttacgac gatccccata gttatgagga
     3061 gctcttctgg ccgatgctgg tggagggcat ttttctggga tcgtttttta cgcctgtgac
     3121 agtgttgacc ctgcacggcc tgtccggtcc gctcatcatg cgcgcggccg aggtcgcgaa
     3181 tctcttacgc gtggcggcgg gtgccttcgg gatcacgttt caaggcatcg tgctgttcag
     3241 gcgtatccat tttcatcaac tgcatctggc cgatcacttc ggcgggcgcc agtcgatctc
     3301 tttcgatccg atggggcagt tgacggccaa actcagcgcg gcaggattgt cgcaggctca
     3361 gatccaggcc aagcttggac tgctgatccg gcaggaagcg gcgattcttg gcctgaacga
     3421 cgcgtttctc gttgcgagtg tcttgtttgt gggattgggc attctcgtgt ggttcgctca
     3481 tccgacccac ttaccggtgg cgccggctcc ggccgatgag ttgcgggaga tgcgcgctga
     3541 ggaaatgatg gaggaggtgc catgattttg cctgtcacaa tgaggcgccg attgcagatc
     3601 gtcctcggga tcggctgcgc gcttttcttc ggcggttgtg cctggatccc gaaaggggat
     3661 caaccggcgc aatatctcga ggcgcctgaa atgacggaga cgctggcgga ggtcacgtcc
     3721 cggcttcaga attggccgga ggatcgctgg tgggaacagt tcggcaatcc ggaactgaac
     3781 gggttgatcg agcagtcgct cacagataac cccgggttaa aacatgcggc ggcacggctg
     3841 cggcaggcca tgtcactcgt gaaggtcgaa ggtgcacggc tgttgccgtt tctcgaagca
     3901 gatgcatcgt tgacctacga gcgcatttca caacatggcg tgtttgcagc cctgaatccg
     3961 gaagtggcgg gcatcaggat catgtacggg attatcaatc cgttgagctt ccggtatgag
     4021 ttcgattttt ggggaaagaa ccgggccatg ctggaagccg ctctgggcca tgctgcggcc
     4081 gaagaagcgg aaacggccga agttcgacta cggctgacca ccggcattgc ccgcgcctat
     4141 ttccgcggtc aggcgcttca acagcagctt actatcgtca agaccattgt cggcatccgc
     4201 cgcgagctga agacactggc ggagactcgg ttccggctcg ggcttgataa cgaccagccg
     4261 gtcaaaatcg cggtggccga ctatgaagcc gcgttcaagc gccaggcggc gatccgcgat
     4321 cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcggtg gccgactatg
     4441 aagccgcgtt caagcgccag gcggcgatcc gcgatcagtt ggatgtgcag cgccatttac
     4501 tggcgcgatt agtcggcaag gggcctgatg agggggccca tctgttcgcc aagccgaaag
     4561 tgatcgttcc cagtcagatt gcggcaccgg atcatctgtc cctgggatta ctcgtccatc
     4621 gtcccgatct ggcggccgcc ctctatcgcg cccacgccgc ctcgcgtttg gtcagggtgg
     4681 cgacgacgca gttctatccc accatcgatc tgacgggatt cgtgggattc aatgcactga
     4741 cattggcgaa gggtacggat aagctggcca attttctttt cagcggtcaa agcttcacct
     4801 atggcttggc gcccggattg cgcatgccct ggtttgaagg cggccggctc cgcggtgaat
     4861 tgggtgcgca acgggcggaa tacgatgcgg cggtggagct ctacaacgac acgctgctgg
     4921 aagcgatgcg ggaagtcgcc gacagtttga gtgcctggca aaccacgaac gaaatcatgg
     4981 cctcgcacaa acggctggtg gcctcgttgg gtgatgactg gcgtttggcg aaggtgcgac
     5041 tcgtgaatgg gctcgatgat gatcgggagg tattgagaca tcgttatccg gtcctggagc
     5101 aggagtatgc gctcagggcg ctggagagcg accaactggt ggcggcagtc gatctgatcg
     5161 aatccttggg cggcgggtat cacaatccgg acattgaaaa acgaccgaaa cagaatccga
     5221 gctaaatcca tgacaaccac aactgcagat atgtctcagc atccgcaatc cgctggtggc
     5281 tcgccgccgg ccggacccta ccgcatccat ccgaaggcca ttcgtgcgcg acggaatcga
     5341 cggctattag tcgtggccgg tctggtcctg gtcgctaccg tcgcgtatct ggtgcactgg
     5401 tatacgcacg accggttttg ggtacgaaca gataacgcct acgtcacagg gaatctagtg
     5461 ccggtggcgg cgcaggcgtc cggcatcgtg acgcaggtgc tcgccgagga aacgcagttc
     5521 gtcaatcggg gggacttgat gatcaagttg gatgagcatc aagcctacgc tgcactgggg
     5581 agggcccgtg gccggttggg cgaggaagtc cgtcgtatcg cgtcgttgtt tatcaatcgg
     5641 aagcaacttg cggaaaaact gcgctcgcgc cgggcccggc tggagttggc cgagcacgac
     5701 atggatcggt atcaacgggc cgctccgagc ggcgcgatct ccaaacagat cctgcaaaat
     5761 accgcggaca aaattgcgag tttggacgca gaggtgcggg agacccaggc cgagctggat
     5821 tctctggatg cgcagatcgg gggcacgacc gtcatggccc atccggcagt agatctcgcc
     5881 aagcatcagc tgatcgaagc gcacctggag tatacgcgcc aacagatccg ggctcccatt
     5941 tccggatatg ttgccaagcg caaagcccag gtgggagatc gtgttcagcc cggggcgctc
     6001 ctcatgacca tcgttccgct ggatcatctg tgggtcgaag cgaatttgcg tgaaacggaa
     6061 ctgcagcatg tccgtcccgg ccagccggcc ctggtgaacg tgagcctcta cggatcgaag
     6121 caaacgttcc atggcacggt cgagggtctg gtgccgggga gcggcagcgc gttcgccctg
     6181 ttgcctccgg acaattcgac cggaaacttc attcacatcg tcgagcgcgt cccggtgcgt
     6241 atcgccttgc ccgcagacga actgcgggaa catccgatcc ggccgggtct gtcgaccgtg
     6301 accagtatca acatcaccga gtccgggcaa tccgtgtgga cctcgttggc gaccccctcc
     6361 acggccgaat acgagaccga tgtctacgcg gacgaattgc ctatggcgga gtctctggca
     6421 aaggaagtga tcgcgaccaa tttggtggtc ggcgatcgcg gagtgtcgat cgattccctt
     6481 ctcgacgagg aggaggagat tcagcggata gtgccacgaa aaggcgatgc gcgcacgtcg
     6541 cctgaaggac gggttccggt ggaccgcgac cggatgcgta cgcaccgtga tccggtgccg
     6601 agttcattca ttccgcgaag aagtcccgat ttgggcgcga cgacggatcc tctcaccccc
     6661 tcaatcggtc caggctccgg gtccttgggg cccgaggcag ggcgcagtcc ctcgcgactc
     6721 aggtcaggtt ttgagatggg tgagggcagg caggtcttgg gcacaggacg tcattagttt
     6781 ccctatcgga acaccgggaa gacaagcctt accgaaccac gtgatccgct aggtggccgc
     6841 cggcgctaca ttaaagtttg atgaagattc gacgagcgac tggaatcaga aatgggccca
     6901 gcctatgatg cccatagcgc acaagccacc accgaccatc gaggcctgct tgcagttcag
     6961 tgctgtgcgt gcatgcgcct ttcctgacga cgtgtcgctc cagcgcacag tgtcagtgag
     7021 ccgttcatag agggtctatg ttacgggaaa aagaaacgcg ccgtgcataa cgcggcgctc
     7081 aactatcgca ccctgttgtc cgtcaccaat gtgctgaatt ctcagcgcaa ccggcagagt
     7141 ctgtttcgcg ccgtgacgga tcagttggcg aaagtcgtcc ggtgggagcg agctggaatc
     7201 acggtgtacg atttgaagtc ggatgctttt cgcttttatg cggtggaaac gaatctgccc
     7261 aaggtggtgc tcagaagcga cgcgatgatc cctcgcgcgc acagcgcggt ggggtgggtc
     7321 tacgatcatc atcaggtaca cgtgcggcct caccttcagc gagagcgact gtttatcgag
     7381 gatgagagtt atctccagga aggacttggt cgcatgatca accttcctat ggtcgtccag
     7441 gaagcgtgtc tcggtactct gaacatcggc agcgtcgagg cgggacatcc ggatccggcc
     7501 gatgtggatt ttctccagca ggtggccacg cagatcggct tcgccatcgc tcacgtcaac
     7561 gcctacgaag aaatcaaccg tttgaaagaa cagttggcgc gggaaaacgt gtacttggcg
     7621 gaggagctga aggctaatca ggattttggc atcgtcgtag ggcgaagcca ggcatttgag
     7681 caggtgctga cgctcgctcg gcaggcggct ccgacgggcg ccacggtgat ggtgacaggg
     7741 gagaccggaa ccggcaagga ggtcctggcg cgcgccatcc acgagcggag cttgcggcgt
     7801 gatcgggcct ttgtgcggtt gaattgtgcg gcgctgccat cgggattgat cgaaagcgag
     7861 ctgttcggcc acgagcgggg cgcctttacg ggtgccgaac atcagcgggt cggccggttc
     7921 gagcttgccg atggcggcac attgtttcta gatgaggtcg gcgagatgcc gcttgaggcg
     7981 caggcgaagc tcttgcgggt actccaggac ggtctggtcg atcgagtcgg cggtgcgaat
     8041 gccattccgg tggatgtccg ggtgattgcc gccacgaacg ccgatttaca ccgggcgatt
     8101 caacaggggc gtttccgcag cgatttgtac taccgcttga acgtgtttcc catccatatg
     8161 cctccgctca gagaacgccc ggaggacatc cctatccttg cccgacattt cctgcgggtt
     8221 cattcccagc ggctgaagcg acactgtgaa gacttcgatg ggccgtcgat ggagcggttg
     8281 gtgcaatatg cctggccggg aaatgtgcgg gagcttgaga acatcgtgga acgtgcattg
     8341 atcctgtgtc acgagcgcct gttgcgtatc gatccggcga tggtgaatgt acgtgtcccg
     8401 cccgaagcag gtcctcgccg aacgctgcat gacgaagagc gacaccatat tcttcaaacg
     8461 ctcacgctgc tcgactggcg gatcgaagga cccggcggag ctgcggacca gttggggttg
     8521 gccccgagca cgctccgcag ccggatgcag aaattggcca ttcgccgccc ctcccgcatg
     8581 taaccagttc ctccgtgcct ctctctctct ctctctctga tcacggtatg aatgttccag
     8641 cccacgattg atcgtggtgg aatcgtggta tcccaccacg tgtcttcgtg gctgttttgt
     8701 gccgcccgcg accttcatga aatcttcatt tctgccgttc gatttttcaa cgacatagac
     8761 attctgtcgg gatccgtcct cgtggcatgt gaggtgcaca aggggtcgtc gtgatttttt
     8821 attcttttca tcttctcact gatgcgcgcg gttcctcggg cagcgagctg agaatcgctg
     8881 cgccgccgca cgagcggccg ctcgtgcggc gggaacacca ccggcctgga aggagggttc
     8941 atttgcattg gcctgttccg gtcatggggg aagtgttgtg cggacgccat gaatcgtggc
     9001 gtcggtagcg tgtcctgtct tcttatgagg aggtattcat gatgcagtta tcccgcaggc
     9061 agttcttgaa ggtctcggcg gggacggtcg cagtggcagc tgtagccgac aaggctctgg
     9121 cgttgactgc gctgcaaccg gtcgtcgagg tcgacaatcc cttgggtgag tatcccgatc
     9181 gatcgtggga gcgcgtctac cacgatcaat atcggtacga ctcatccttc acctggtgct
     9241 gctcaccgaa cgacacccac gcctgccgca ttcgggcgtt cgtccggaac ggtgtcgtga
     9301 tgcgcgtgga gcagaactat gaccaccaaa cctatgaaga tctgtacggc aaccgcggca
     9361 cgttcgcgca taacccgcgc atgtgcttga agggcttcac gttccaccgt cgtgtctacg
     9421 gtccctatcg tttgaagggg ccgttgatgc ggaagggctg gaaacagtgg atggacgacg
     9481 gctcccctga gctgacgtcg gatgcgaagc ggaagtataa gttcgacagc cggttcttgg
     9541 atgacatggt ccgcgtgtcc tgggatacgg cgtttaccta tgtcgccaag gggttgatcg
     9601 tcatcggcac tcgctacagc ggtgaagccg gtgcccgtcg cctccgcgaa cagggctatg
     9661 ctccggaaat gatcgagatg atgaaggggg cgggcgtgcg gacgttcaag caccgcgccg
     9721 gtatgccgat cctcggcatg atggggaaac atgccaatac caggttcaac aactgcgtgt
     9781 tgcccttgtt ggatagctgg atccggaagg tcaatcccga tcaggcgcaa ggcggccgct
     9841 actggaaca
//
LOCUS       DAOQRM010000092         9817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6246_length_9817_cov_19.346958, whole genome shotgun sequence.
ACCESSION   DAOQRM010000092 DAOQRM010000000
VERSION     DAOQRM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6246_length_9817_cov_19.346958"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PKK81_08505"
     CDS             <1..1131
                     /locus_tag="PKK81_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HNV32957.1"
                     /translation="NRKYVPPIDTQAEYTIAWTEEALRRMERIPVFARGVAKTAIHRY
                     AIEKGHTIISNTVVDSAVGHILPKGAMDAMRALGGNLDAAGIDRDKMQADDSVAQDLM
                     GSTLSGMMTQVVEEKPVNSPGTQAYLDRMSQNYFVCDGCGYIGKGDTPVKCPVCSAEG
                     DRFKQVDKTIFEAAAKAEGGLETDLAYDDVPMQWTKDAKEAIRAVPAGFQRRRAKAKI
                     EKSARKLGMTTITLEYAGPMIQEAAAEDYTPIFANKGTGTTPQQSPEAAETNGADAHA
                     KNGHGEEPAPVPQETVSPYTWTPDAQGRLDRAPEGFMRECTKALIEKHADKIGTTVIT
                     LEVATEGIEQAKGYMADAMKTGNLKDMIANLTGSSSKTGANR"
     gene            1131..2534
                     /locus_tag="PKK81_08510"
     CDS             1131..2534
                     /locus_tag="PKK81_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV32958.1"
                     /translation="MGKSLPVLNFPFLGDKLESLQQQISKFISPTSPSAAPNDGRTVD
                     DFKPYLVALNLTKRCNLKCDHCYLDATTKAGGGSDELSTEECFRLIDQIAEVNKGCLL
                     VITGGEPLVRPDILDIARHAVGLGFIVVFGTNGMLINDRMAKTLVEIGVMGVGISIDS
                     LDPDKHDQFRGVPGAFASALAGIEACKRNGLQFQVHFSAQPMNYQELPAVVDWAHTLG
                     ARVLNVFFMVCTGRGEELTDITPQQYEEVLGYLVDCQDQYKDMLVRARCAPHFKRLAY
                     EKDPNSPLTKATGYMGGGCLAGTNYARVTPNGELTPCPYMPLSAGNVRETSFVDLWER
                     SDVFNSFRYPQLKGKCGDCEYTDICGGCRARPYVDHGDWLDEDQWCLYTPKGGDKIKV
                     AFNTPEETDIAWDEASALRLSRIPYFLRAMVKKGVERHARETGIAMVTVELMEELRKK
                     RFGNDAPVFNFDMRGKD"
     gene            2605..3120
                     /locus_tag="PKK81_08515"
     CDS             2605..3120
                     /locus_tag="PKK81_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNV32959.1"
                     /translation="MDSVQSIFLDLNQLIPIVNHWLHLLSAVIWIGGLAFIVMAVKPG
                     LREAALPKEYVRPITDAFYKHYKRVAGILLVVLLFTGGINLHYVNQIFVSQTGQGMQN
                     HPKYLWIFCIKLILVLCLLTLFLYTVIFKSDQDEEEEGENYESIPFQRAALWMGFFII
                     LCAAAMKHLHQ"
     gene            3473..5206
                     /locus_tag="PKK81_08520"
     CDS             3473..5206
                     /locus_tag="PKK81_08520"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HNV32960.1"
                     /translation="MPFYSQRIVVLAGLLFALSTVHCQAIDLPPPHTVSSSDTQGWTE
                     QDRQQWYHTSAGTQLLPYDWFVVLEDEPLKNSFARTGIIPDQTHPDRLPIGFTKTEGP
                     NVPEPTVGLTCAFCHTTQFTYQGNPIRIEGGPSLQYNQRFLQVLLESLGELKAPDKFQ
                     AFAARVLQRRGQAVTQENIATLAGQFSQVMKDLVARGGRDASPALWGPGRFDALGRGG
                     NTVFSPLNPDNLRPANAPVSIPPLWGVWEYDWVQWAGSIQHPLARNIAQVIGVNAALF
                     SWARTSPQPPSEKKEIFRSSIDVASLKTLEGLAKRLHPPRWPKSFPPINRELAARGKD
                     LYHGNKLKGLPNLCAHCHVATKLDSPNPNGPSLHVTMIPQKEVGTDSLYLENFSRRTV
                     DLERLGLGRLSAKDASQLVTTELMTLNGAGSDPEYQHLTNTWRDKAQYIARPHLAVWA
                     TAPFLHNGSVPNLYALLSPVKERPACFYLSPNMEFDPVKVGFVVSECNDSPTFRDPLV
                     GFEFKTHLPGNSMEGHEFKGSDCGSVVAGAGVLGCEIPIADRWAIVEYLKTCDLDRLV
                     IHDAPACRDLE"
     gene            5351..8068
                     /locus_tag="PKK81_08525"
     CDS             5351..8068
                     /locus_tag="PKK81_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV32961.1"
                     /translation="MTDLLSKEHSILERPFMEFRPFGLNDQGEKICDISGVIVQSNVD
                     YMCDYLSRTAGKAAATQALDELCRLLNARLPDRTYHVTPDSLRNIWNSYSYEFVCYLR
                     EFCEQLSGDPQFHVNVGTNRKVPPLIQILCRPFSTPQLYKMWPYFGSKYVRGVLEFEV
                     GRVTRRSAVLRMTFTDRALVQFGPYRKRCVDVICLSCKSSIARVQTQMHGTPAATVRD
                     LSCTANGDPYCEWEFTWTPQVRSSVPLAMWMLVAGGTFTYLHLRQPDVPTVEALGLAA
                     IPASLLWWMITNHMRQAAKPLQRLIKDQEQVVDARHEELREAYLEQQSTAVTLRRKIN
                     DLTTLHRAGLLFSSTFDREELLTNVLETIVRELHYDRAMITQFDRTRNVSHDFRVRGM
                     PLEVADFLRTQEVPVTDPNSVEGTVFLRGEPVLTNNIHEIWDRLHPFDQKLIAMVNVK
                     SFITVPLKTQDAVIGSLSVDRTHNQALTQDDLDVLVTLASQVAIALDNAQAYREIESL
                     NAGLEARVRERTAELEAANAQLKQMDRLKSKFLAHVSHELRTPLTSIVGFADNMLEGL
                     VGSLNMKQEQYLTRIKANGTRLARMITDLLDLSRVEAGKLVLSFEQVCLPTIVSDVIE
                     QLLPLAITKHLNIELHSPDPELLVWADQDRLSQILTNLLDNAIKYTPDGGQVAVELSV
                     DTHDMARIVIRDNGQGIPPDALPKLFDPFFRVQQEERSQTKGLGLGLAIVKDLVDLHG
                     GSITVRSEVDQGTEFTFTLPLHSREPSPAGQLPAVRRTVLVVDDDPDICDLLRDRLEA
                     EGVHVCTAADGRAALRMLAETTVDGVLLDIALPELDGFEVLRQLRPTHPTLPVVMMTA
                     VEALDRAMAAVESGAQDYLLKPFDGTRLRQIVDRWFVIGSGQSESPIGR"
     gene            8073..9461
                     /locus_tag="PKK81_08530"
     CDS             8073..9461
                     /locus_tag="PKK81_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNV32962.1"
                     /translation="MPLAQRFRRALLLCLLCSAAGFVFAAASANAQTPRLQGQSAAAA
                     AMGNAFVAQADDPSALHYNPAGMTQLHGFQTLFGTTLIGGTTQFTSPTGAQVTGDRNG
                     SVAWPPPGHVYLVANLKDLGLSALGNFTAGIGMNNPFGSLTRYPNDGPFRTAITFTTL
                     PLLDIKPTIAYKLNDQLSFGLGADIYTFSGLFGEGHLEQKSIWPGGLGIPAGSLMEIN
                     GSDTTAGFNVSLLYTPFRNSDGKPLVNIGMVYRSQATFHLTGNFLANGASVAGATATF
                     VLPQVFSGGIGIWPVRTSEREWKLEFDVDYTGWKSNRNLDVHLTNGALLPFPQNWQST
                     YTIMIGTEYRWLRLASMPDWDIALRAGYMNQQAQIPDRTFNPGVPSANTHIPSVGIGL
                     ACHENGSFLGLVRCGELGIGPLKPKLFAIDLAYQAGLYEVRTIAGNQNPTVNGRYDSM
                     VHVGSLSLRFNY"
BASE COUNT         2112 a         3033 c         2677 g         1995 t
ORIGIN      
        1 aatcggaagt atgtgccacc gatcgatacg caggcggaat acaccattgc ctggaccgaa
       61 gaagccctgc gccggatgga acggattccc gtgtttgccc gcggagtggc taagaccgcc
      121 attcaccggt acgctattga gaagggccac acgattatca gcaatacggt cgtcgactct
      181 gccgtcggac atattctccc caagggagcg atggatgcga tgcgggcgtt gggcgggaat
      241 ctggatgccg ccggaatcga tcgcgacaag atgcaggccg acgattcagt cgcacaggac
      301 ctgatgggtt ccaccttgag cggcatgatg acccaagtgg tcgaggaaaa gccggtcaac
      361 agccccggta cccaggccta tctggatcgc atgagccaga actactttgt ctgcgacggg
      421 tgcggatata tcggaaaggg cgacactccg gtgaaatgcc cggtgtgcag cgccgaaggg
      481 gatcgcttta aacaagtcga caagacgatc ttcgaagcgg ccgccaaggc cgagggtggg
      541 cttgagaccg atctcgccta cgacgatgtc ccgatgcagt ggaccaagga tgcgaaggaa
      601 gcgattcgcg ccgtcccggc cgggttccaa cgtcgccggg ccaaagccaa aatcgaaaag
      661 agtgcccgca agctcggcat gaccacaatt acgctggaat atgccggtcc gatgattcaa
      721 gaggcggcag cggaagacta tacgcccatc tttgcaaaca agggtacggg aaccactcct
      781 caacagtccc ctgaggcagc cgaaaccaac ggagccgacg ctcatgccaa gaacggccat
      841 ggcgaagaac cggctccggt tcctcaggaa accgtgtcgc cctatacctg gacgccggat
      901 gctcagggtc gtttggaccg cgcaccggaa gggtttatgc gggaatgcac caaggccctg
      961 atcgaaaaac atgccgataa aatcggcacg acggtcatca ccctggaagt ggcaactgag
     1021 ggaatcgagc aggcgaaggg ctacatggcc gatgccatga agaccggcaa tctgaaagat
     1081 atgatcgcca acctgaccgg ctcctccagc aaaaccgggg cgaaccggtg atgggaaagt
     1141 cactgccggt tctcaatttc ccgttcctcg gagataagct ggagtctctc cagcaacaaa
     1201 tctcgaagtt catctccccg acttccccct cggctgcgcc gaacgacggt cgcacggtcg
     1261 atgactttaa accctatctg gtcgcgctga acctcaccaa gcgctgcaac ctcaagtgtg
     1321 atcactgcta tctcgatgct acgaccaaag ccggcggcgg gtcggatgag ctcagtacgg
     1381 aagagtgctt ccgtctcatc gatcaaatcg ccgaggtgaa caaaggatgc ctgctggtga
     1441 tcaccggcgg agaaccgctg gtgcgtcccg acatcctgga catcgcccga catgccgtgg
     1501 ggctcggctt catcgtcgtg ttcggcacca atggcatgtt gatcaacgat cgcatggcaa
     1561 aaacgctggt cgagatcggt gtcatgggcg tcggcatcag catcgactcg cttgatccgg
     1621 acaagcacga tcagttccgc ggtgtccccg gtgcctttgc cagcgcattg gccggcatcg
     1681 aggcctgcaa acgcaatggt ctgcagttcc aggttcactt cagtgcgcag cccatgaact
     1741 atcaggaatt gcctgcagtc gtggactggg cccataccct cggcgcacgg gtcctgaacg
     1801 ttttcttcat ggtctgcacc ggacgcggcg aagaactgac ggatatcaca ccccaacagt
     1861 acgaagaggt tcttggctat cttgtggatt gccaggacca gtacaaagat atgctggtac
     1921 gcgcccgctg cgctccgcat ttcaagcgac tggcctacga aaaagatccc aattcccctc
     1981 tgaccaaagc caccggatat atgggcggag gctgtctggc agggacgaat tacgcacgag
     2041 tcactccgaa cggggaactg accccctgtc cctacatgcc gctatcggcc ggaaatgttc
     2101 gcgagacgag ttttgtcgat ttgtgggaac gctcggatgt gttcaattcc ttccgctacc
     2161 cccaactcaa gggcaaatgc ggcgattgcg agtacacgga catctgcggc ggctgtcggg
     2221 ctcgacccta cgtagatcac ggcgactggc tcgatgaaga ccaatggtgc ctgtacaccc
     2281 ccaagggcgg agacaagatc aaggtcgctt tcaacactcc ggaagagacc gatattgcat
     2341 gggacgaggc ctcggcgctt cgactcagcc gaatccccta tttcctgcgg gccatggtca
     2401 agaagggcgt ggagcgccat gcgagagaaa ccggaatcgc gatggtgacc gttgagttga
     2461 tggaagagct gcggaaaaag cgatttggaa acgacgcgcc ggtcttcaat ttcgatatgc
     2521 gagggaagga ctaggaaccg ggcgccacgc aggacctacg gtagccccag ctacattcgc
     2581 tggtcgcccc tgaccgatct ataaatggat tccgtacaaa gtatcttcct tgatctgaat
     2641 caactgattc cgatcgtcaa tcactggctc catcttctct cagctgtgat ctggatcggc
     2701 ggcctcgcgt tcatcgtgat ggccgtcaag ccggggcttc gagaagccgc cctacccaag
     2761 gaatatgtcc gcccgatcac cgacgccttt tacaagcatt acaagcgggt ggcggggatc
     2821 ctgttggtcg tcctgctgtt cacgggcggc atcaacctcc actacgtcaa tcagatcttc
     2881 gtctcgcaga ccggtcaggg catgcagaac catcccaagt acctctggat tttctgcatc
     2941 aagctgattc tggtcttgtg cctgctcacg ctgtttctct acaccgtcat tttcaagtcg
     3001 gatcaggacg aggaagagga aggcgaaaac tacgaatcga ttccgtttca acgcgccgcg
     3061 ctctggatgg gcttcttcat catcctctgc gccgctgcca tgaaacatct ccaccagtag
     3121 caagttcact tcttccgcat atcgttttca tcaactgcgc tgtatctctt cagatacccc
     3181 atgcgcttga cccgctcttc cctttccata ccggacgaga cgggcttcaa acctgccacc
     3241 cgccacgcat cgcactcaaa aaataaatgg ctttcgtgta ccgatccagg tacccttccc
     3301 gcgcttatgg catatgagtt gcttctcagc acctgagtga gtgcggcgct tgcctccatc
     3361 agtgccgtcg caatcagcga atgaatgatg catgcgcgtc aggttgacca acctctggat
     3421 caatcatgca ctacccgttc agataatctc gcccaccatg aggagatgga ccatgccttt
     3481 ctactcccaa cgtatcgtgg tacttgccgg gctgctgttt gcactctcga ccgtacattg
     3541 ccaggcgatt gatctgcccc ctccccacac agtcagttca tccgacaccc aaggatggac
     3601 ggagcaggac cggcaacagt ggtaccatac gtccgccggc acccagctgc tcccttatga
     3661 ttggtttgtc gtcctggagg acgagccact caagaacagc ttcgcccgaa ccggcatcat
     3721 ccccgaccag acccatcccg acagacttcc aatcggattt acgaagaccg aggggccgaa
     3781 cgtcccggag ccgactgtcg ggttgacctg tgcgttctgc cacacgactc agttcaccta
     3841 ccaaggcaac ccgattcgta tcgaaggggg tccgtccctc caatacaacc agcggttcct
     3901 acaagtactc ctggagagtc ttggagagtt gaaagccccc gacaaattcc aggcctttgc
     3961 agctcgtgta ctgcaacgcc gtggacaggc ggtaactcaa gagaatatcg ctaccttagc
     4021 cggccaattc tcacaggtca tgaaggatct cgttgctaga gggggaagag atgcctcacc
     4081 agcactgtgg ggcccgggac ggttcgatgc gctcggtcgt ggcggcaata cggtcttctc
     4141 gccgctcaat ccggacaacc tcaggcctgc caatgcaccg gtgagtattc cgccgctctg
     4201 gggagtatgg gaatatgatt gggtgcagtg ggccggctcc attcaacatc cgctcgccag
     4261 aaatatcgcc caagtcattg gggtgaacgc cgcactcttc tcttgggcaa gaacctctcc
     4321 gcagcctcct tcagaaaaga aggagatctt tcggtcgtct attgatgtgg cctcgttgaa
     4381 aacattggag gggcttgcga aacggctgca cccaccaagg tggccgaaga gcttccctcc
     4441 gatcaatcga gagttggctg caagagggaa agatctctat cacggcaaca agctgaaggg
     4501 ccttccgaat ctctgcgccc attgccatgt cgccacgaag ctcgattcgc caaacccaaa
     4561 cggcccaagc ctgcacgtca cgatgatccc ccagaaagag gtcggcacgg acagcctgta
     4621 tctggagaat ttttcccgac gtaccgttga cctcgaacgt cttgggttgg gccgattatc
     4681 ggcaaaagac gcctcccagt tggtcaccac ggaattgatg accctcaacg gcgcggggag
     4741 cgaccctgag taccagcatc tcaccaacac atggcgtgac aaggcccaat acatcgcgcg
     4801 cccgcatctt gccgtctggg caaccgctcc attcctacac aatgggtccg ttcccaacct
     4861 ctacgcattg ctctctccgg ttaaggaacg gccggcctgc ttctatctca gtccgaacat
     4921 ggagttcgat ccggtgaagg tcgggttcgt cgtctcggaa tgcaacgatt ctccgacctt
     4981 ccgcgatcca ttggtaggat ttgaatttaa aactcacctt ccgggaaaca gcatggaagg
     5041 gcatgagttc aaaggatctg attgtgggtc tgtggtggcg ggcgccggtg tattaggctg
     5101 tgagataccg atcgctgatc gttgggccat cgtcgaatac ttgaagacct gtgacctaga
     5161 ccgtttggtc atacatgatg ccccggcctg ccgggatctt gaataacgga tcacagccgg
     5221 gtgaccaggc gcgcaccgac agacgcgacg ctgcacggag ggcctcgcct cccgagcaca
     5281 ggtacgttat gatctggccg ccggggcact cctctgtcac gcggcatcga ccgaatcggg
     5341 cggcttccgt atgaccgacc ttctctccaa agaacactcc attctcgaac gaccgttcat
     5401 ggagtttcgc cccttcggcc tcaacgacca aggcgaaaag atctgcgaca tcagcggggt
     5461 catcgttcaa tcgaatgtgg actacatgtg cgactatctg agtcgcaccg ccgggaaagc
     5521 cgccgccact caggctctgg atgaactgtg ccgattgctc aacgcccgcc ttccggaccg
     5581 cacctaccat gtcacccccg actcgcttcg caatatctgg aatagttact cctacgaatt
     5641 cgtctgttac ctcagagagt tctgcgaaca attgtccggg gatcctcaat ttcacgtcaa
     5701 cgtgggaacg aacaggaaag tccccccctt gattcaaatc ctctgtcgtc cgttttcgac
     5761 cccacagctc tacaaaatgt ggccctattt cggcagcaag tacgtccggg gtgtgttgga
     5821 gttcgaggtc ggccgggtga ccagacgttc ggcggtcctc cgcatgacct tcaccgatcg
     5881 ggcccttgtg caattcggtc cctaccggaa gcggtgcgtg gatgtaatct gcctctcctg
     5941 caagagcagt atcgcgcggg tgcagacgca aatgcacggc acaccggccg ccacggtcag
     6001 ggacctctcc tgcaccgcca acggcgatcc ctattgcgaa tgggaattca catggacgcc
     6061 gcaagtccgt agttccgtgc cgctcgccat gtggatgctc gtcgcaggag gcacattcac
     6121 ctacctgcat cttcgccagc ctgacgtgcc gacggtggaa gcgctggggc tcgcggctat
     6181 tccagcgtcg ctgttgtggt ggatgatcac caatcacatg cgacaagccg ccaaaccgct
     6241 gcaacggttg atcaaggatc aggagcaggt cgtcgatgcg cggcatgagg aattgcgcga
     6301 ggcctacctc gaacaacaga gtaccgccgt caccttacga cgaaaaatca atgacctgac
     6361 gacacttcac cgtgcaggac tgttgttcag ttcgaccttc gatcgggaag agctgctgac
     6421 caatgtttta gagacgatcg tccgtgaatt gcactacgat cgcgccatga tcacgcagtt
     6481 cgatcgcacg aggaacgtgt cgcacgactt tcgagtgcgc ggcatgccgc tggaggtcgc
     6541 ggattttctg cgtacgcagg aagtgcccgt caccgacccc aacagtgtgg aagggaccgt
     6601 gttcctgcgt ggcgaacccg tcctgacgaa caacattcac gaaatctggg accggctgca
     6661 cccgttcgat caaaagttga tcgcgatggt gaatgtaaaa tcgttcatta ccgttccact
     6721 caagacacaa gatgccgtca tcggctctct ctcggtcgac cgcacgcaca atcaagccct
     6781 gacccaagac gatctcgatg tcctggtcac cctcgccagc caggtcgcca tcgcgctgga
     6841 caatgcccag gcgtatcggg aaatcgaatc gttgaatgcc ggcctggagg cccgcgtacg
     6901 cgagcgtacg gcggagttgg aagccgccaa cgcgcaactc aagcagatgg atcggctcaa
     6961 atcgaaattt cttgcccacg tgtcccacga gcttcgaacg cccctcacca gcatcgtcgg
     7021 cttcgcggac aatatgctcg agggactcgt cggctccctc aacatgaagc aggaacagta
     7081 cctcacccgc atcaaagcca acggcaccag acttgcccgc atgatcaccg acctcctgga
     7141 cctctcgcgt gtcgaggccg gtaaactcgt gctctctttc gaacaggtgt gccttccgac
     7201 gatcgtgagc gacgtcatcg aacaactcct gccgctcgcc atcaccaagc acctcaatat
     7261 cgaacttcac agtccggatc ccgaactgct ggtgtgggcg gatcaggacc ggctcagcca
     7321 gattttgacg aatttattgg ataatgctat caagtacact cctgacgggg gacaggtcgc
     7381 ggtggagctt tcggtcgata cgcacgacat ggctcgtatc gtcatccgtg ataacggcca
     7441 aggcatcccc ccggatgcgc ttcccaaact ctttgatccg ttcttccgcg tgcagcaaga
     7501 agagcgaagc caaaccaagg gattgggact cgggttggcg atcgtcaaag acttagtcga
     7561 tctacacggc ggcagcatca cggttcgtag cgaggtggat cagggaacgg agtttacctt
     7621 cacccttccc ctgcattcgc gcgaaccatc tcccgccggc cagttgcccg ccgttcgccg
     7681 cacggtgttg gtcgtcgacg acgatcctga catttgtgac ctgctgcggg atcgcctgga
     7741 ggcggaaggc gttcatgtct gcaccgcggc ggacggtcgc gccgcattac ggatgctggc
     7801 ggagacgacg gtcgatggag tgttgcttga tatcgccctg ccggaattgg acgggtttga
     7861 agtcctgcga cagttgcgtc ccactcaccc cacgttgccg gtggtcatga tgacggccgt
     7921 ggaggcgctg gatcgcgcca tggcggcagt ggaatccggc gctcaagatt acctcctgaa
     7981 gcccttcgat ggaaccaggc tgcggcaaat cgtcgatcgt tggttcgtca tcggctccgg
     8041 tcaatccgag tctccgatag gacggtaacc ccatgcctct cgctcagcga ttccgccgcg
     8101 ccctgcttct ctgcctgttg tgttcggccg ccggattcgt cttcgctgcc gcttccgcca
     8161 acgctcaaac cccacggctc cagggccagt ccgctgccgc cgccgccatg gggaacgcct
     8221 tcgtcgctca ggccgatgac ccttccgcgc ttcattacaa ccccgccggc atgacacaat
     8281 tgcatggatt ccaaaccctg ttcggaacca cgctgatcgg gggcaccacc cagttcacga
     8341 gtcccaccgg cgcacaagtg acaggcgatc gcaacggaag cgtggcctgg ccccctcccg
     8401 gccacgtgta cctggtcgcg aatctcaagg acctcggcct gtcggcactc gggaacttca
     8461 ccgccggtat cgggatgaac aatccgttcg gttcattgac cagatatccg aacgatgggc
     8521 cgtttcgcac ggccatcact ttcaccacct tgccgttact cgatatcaaa cccaccatcg
     8581 cctacaaact gaacgaccaa ctctcgttcg gattgggcgc ggatatttac accttttccg
     8641 ggctcttcgg agaaggccat ctcgaacaga aatccatctg gcccggcggg ctgggcatcc
     8701 cggccggctc cctgatggag atcaacggaa gcgacaccac ggcaggattt aacgtcagcc
     8761 tcctgtacac ccccttccgc aacagcgacg ggaaaccact cgtcaacatc ggcatggtct
     8821 atcgcagtca agccaccttt catctcacgg gcaactttct cgccaacggc gcctcagtcg
     8881 ccggcgccac agccaccttc gtcctgccgc aagtcttctc cggcggcatc ggcatctggc
     8941 cggttcgtac gtccgagcgc gaatggaaac tggaattcga tgtggactac acgggatgga
     9001 aatccaacag gaacctcgat gtccatttga cgaacggcgc cctgttgccg tttccgcaaa
     9061 attggcagag cacctatacc atcatgatcg gcaccgaata ccgatggctg cgcctggcct
     9121 cgatgccgga ttgggacatt gcccttcggg ccggctacat gaaccagcag gcacaaatac
     9181 ccgatcgcac ctttaatccg ggagtcccct cagccaatac ccacattccc tccgtcggga
     9241 tcgggttggc ctgccatgaa aacggttcgt tcctcgggct ggtgcgttgc ggcgaactgg
     9301 gcatcggccc gctgaagccg aaattgttcg ccatcgatct cgcgtatcaa gcgggtctgt
     9361 atgaagtacg caccatcgca ggcaaccaaa acccaaccgt caatggccga tacgacagca
     9421 tggttcatgt cggctcgctg tcgttgcggt tcaattactg agtccaacgc gttcctgccg
     9481 gctccgatcg ttatgtcctg cccgtttctt cccttcaggc acaacatgcc ccacgcctac
     9541 ggcagaggct cttcggcctc gggacaaccg ttcgtcgatg cgccgcgatg gcctcatctt
     9601 caggcatcga cggcatgacg gttgcttctt ccactgccat gggtcaatgc agccgaggga
     9661 acaggtggat cgaatcgtgc tcgtgccctt tcggcgtggc agagagccgg tttcctgtcg
     9721 ccggttgtca accaagagca accgcatgac acggcctcct ggatcgtgcc catccagcgc
     9781 tgatcagacc gccttctcca cgccatctgc cgcgtct
//
LOCUS       DAOQRM010000093         9581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6512_length_9581_cov_18.144237, whole genome shotgun sequence.
ACCESSION   DAOQRM010000093 DAOQRM010000000
VERSION     DAOQRM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6512_length_9581_cov_18.144237"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="PKK81_08535"
     CDS             <1..1449
                     /locus_tag="PKK81_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV32963.1"
                     /translation="FMEYGSFASLREGGMVSGKQGAVDYSFSLSRWDTSSFSAVNYRR
                     GAGERDSYRNWQGSGRIGVDLPKDGRLDFTMRWMNSDVQLDSVSATSPSDVYGSKNRS
                     QEYVFSGSYEQPLTGWWSQKLTLARAQEASQFLPGTLQRSLITGAFSTPFGTPNEIRV
                     LSNRLEWQHNFQITKLLLLSAGYQFREQQGENDTGLTNRILSSNAGFAQAQFNLWDRL
                     FATAGIRHDSYNVFGDATTYRLTGGYLHKETDTKIRGSYSTGFRAPTMNELYFPNFGN
                     SQLGTEKSQSMDVGIDQYFFSKQLKFTGGFFWNRYRNLITTTFDPTFCAPFSTFGFCP
                     QQIGEASTKGWEAGLSYTYSSDRPFLKGLVVQANYTNTMTRDLVTQTRLPRWATDQWS
                     ASVSYQPIDPLWITLIGRYVGSRYNTTGDRQSLPAFDVWSLAVTYDVTKQLQAYLRAE
                     NLFNEKYEEVASAGTPIRSIFGGVRVTFGGKS"
     assembly_gap    1551..1650
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1651..2624
                     /locus_tag="PKK81_08540"
     CDS             <1651..2624
                     /locus_tag="PKK81_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HNV32964.1"
                     /translation="AVQVRGVLFNRVRSEGHYQLLRQAVESETDLAVVGYLQPDASVT
                     IQDRHLGLRTAIEQGQGDLYERLARAAAQTIDLDRVGAMARSAGECPGEDLPITRPAA
                     TQQPVRVGLAYDAAFCFYYQDNLELLESAGAEIVRFSPLHDDELPVADLLYFGGGYPE
                     LYGETLAANMSMRSAVQTFARRGGAVYAECGGLMYLTEAIRDGAGARHEMVGVFPADA
                     VMRKNGMTLGYRTVEIAESCMLGGAGTVVRGHEFHYSMLEPTGELHHACTLSDAAGKP
                     VGQDGLTMGNTLALYSHLHFGSHPAVVADLLGTARRLRGAQASATKG"
     gene            2626..3225
                     /gene="cobO"
                     /locus_tag="PKK81_08545"
     CDS             2626..3225
                     /gene="cobO"
                     /locus_tag="PKK81_08545"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNV32965.1"
                     /translation="MAEQDEHTAKMQRLKASVDRRIEAAQDEKGLLIVYTGAGKGKTT
                     AALGMALRCLGHGMKVAIVQFIKGAIDTAEERILKSFGDRVTFLRMGEGYTWETQDRE
                     RDTSHAQAAWAKACEFMRDPSYAMVILDEFNIALHHGYVAVTEVLPRLQQRPAMQHVV
                     ITGRGAPEELIEAADLVTEMKQVKHPFRKGIKAQAGVEF"
     gene            3234..3437
                     /locus_tag="PKK81_08550"
     CDS             3234..3437
                     /locus_tag="PKK81_08550"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HNV32966.1"
                     /translation="MTKTCEHCRKTFECVGYQCWCGKIGITEEQMDWIAARFKDCLCP
                     VCLQQVADGILGPQASNGMPHGT"
     gene            3427..4203
                     /gene="bluB"
                     /locus_tag="PKK81_08555"
     CDS             3427..4203
                     /gene="bluB"
                     /locus_tag="PKK81_08555"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HNV32967.1"
                     /translation="MARDKRERLTRTTAESSEPFSKAEREAVYRAIFERRDVRRNFLP
                     TPIPDEVLARLLNAAHHAGSVGFMQPWDFVVVRTDETKRAVKQLFMDTNAAAALRYKT
                     PRADLYRTLKLEGIEESSLNLCVTCSRQRGGPHVLGRSTVRDTDLYSTCCAIQNLWLA
                     ARAEGIGVGWVSILNHAALKHVLGIPRPVKVLAYLCLGYVSEFAKKPDLEAAGWRARL
                     SVQDLVHYEAWGKRVEGKERLPGCESPRSIQERATRAKRG"
     gene            4149..4745
                     /locus_tag="PKK81_08560"
     CDS             4149..4745
                     /locus_tag="PKK81_08560"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNV32968.1"
                     /translation="MRITKVYTRTGDAGQTRLAGGQQVWKDCLRVEAYGTVDELNASV
                     GLVRAMNADAENGSAASTQLEADLRWVQNKLFDVGSILATAPGQTFPNMPEVTAKDVT
                     KLEAMIDRCQEELAPLKEFILPGGGKVSATLHQARTICRRAERICIRLSREEPVAAEL
                     NKYLNRLSDALFVLARWVSKTQGEPEFLWQRESGATAE"
     gene            4750..5286
                     /gene="cobU"
                     /locus_tag="PKK81_08565"
     CDS             4750..5286
                     /gene="cobU"
                     /locus_tag="PKK81_08565"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNV32969.1"
                     /translation="MKKHPATGSHLILVLGGAASGKSQAALDRAGRRGPKAFVATGQA
                     LDDEMKARIARHRETRPTGWITAEVPRDLAAWFRTEGQAYRTVVVDCLTLWLSNVCGR
                     RIAGQEMVPRVEAVLQAIRGTQARVVLVSNELGFGLVPTSRSARAFRDVAGRVNQQIA
                     AAADEVYFVVSGQSLKLK"
     gene            5303..>6306
                     /gene="cobT"
                     /locus_tag="PKK81_08570"
     CDS             5303..>6306
                     /gene="cobT"
                     /locus_tag="PKK81_08570"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNV32970.1"
                     /translation="MTLQEVLTRIQPVDQAIAAQTQARLDRLTKPLGSLGRLEETAVR
                     YATITGEVKPAVPRGVVFTFAADHGVATEGVSAYPREVTPQMVLNFLRGGAGVNVLAR
                     HAGVEVRVVDIGVAYDFGSVSGLIHKKVMAGTNNLLVESAMSRDQAVQALQVGIEVAT
                     EAAREGIGLIGTGEMGIGNTTPSAAITAVMTGRPVAEVTGRGTGIDEAGHARKVGVIE
                     QALDRHRPNRADAIDVLAKVGGLEIAGLAGLILGAAAARVPVVLDGFIAGAAALIAVG
                     LQPLCRDYLIASHRSVERGHQAILEHLRLKPLLDLDLRLGEGTGACLGMSLVQASIKI
                     L"
     assembly_gap    6307..6406
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6407..6922
                     /locus_tag="PKK81_08575"
     CDS             <6407..6922
                     /locus_tag="PKK81_08575"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNV32971.1"
                     /translation="DGLADTLDGLAGGRTAADRLRIMRDPSVGALGATGLFLSLLLRY
                     AGLLALPQPVRLPVLLCMPALGRWAMVSVAWASPYARSEGGLAAAFLTHLSWQHVLLS
                     SLVLACALMAGMGVIAATATIASGALVVVVVRRGCRAWFGGVTGDLLGATNELIEILF
                     LLLIPVLVMAR"
     gene            6919..7875
                     /gene="cbiB"
                     /locus_tag="PKK81_08580"
     CDS             6919..7875
                     /gene="cbiB"
                     /locus_tag="PKK81_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNV32972.1"
                     /translation="MTGGELVLAAALDVVAGDPRWLPHPVRGMGVVISWFDDQVRTFC
                     RSDQSLQIAGACLALGLPAAVYAAATWLIAQAAAFSPLLGQLVGIGLAYTTLAGRDLF
                     DHVQAVSRELQQGNLAGAREAVARIVGRDSATLEEPEIVRATVETIAESTADGIIAPL
                     VYLSLGGAPLALAYKAVNTLDSMVGHHDERYEHFGWASARLDDVMNWVPARLTGGFIA
                     LASGLATAQWHRVQDSWYILHRDGDKHPSPNSGMPEAAMAGGLGVQLGGRNYYDGVAH
                     EGELIGDAGTALTPGHIDQATRIMVVAAGLGLFFALLSLWVS"
     gene            7863..8981
                     /gene="cobD"
                     /locus_tag="PKK81_08585"
     CDS             7863..8981
                     /gene="cobD"
                     /locus_tag="PKK81_08585"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HNV32973.1"
                     /translation="MGIMTRIGHGGDVYAAARELGRDLHRLLDFSASINPLGPSPAAV
                     RTLRTAEVLLGHYPDPACWALRQALAAYWKRPSEEFLVGNGSTELIHLLPRTLGIRHL
                     LLIGPTFSEYAEAMTRAGGRVTMLMADRADGYRPPLESVLATLQKGRQSASGAYPIDA
                     VLLCNPNSPTGQACEAAAVRKCARLVARRGLWCIVDESFAEYANHASILSEPLPPRTV
                     VLRSFTKFYGLPGLRVGYATAKPAVIARIAAQQPPWSVNMLAQRTAVAALKDVRHAGR
                     SLRFMERERTRVQKALTRLPGCTVFPSAANFLLIEVPVGQKATAVVAALRRQGLLIRD
                     CSQVPGLNDRSVRVAVRPRADNDRLLRTLSVLLHKGGA"
     gene            8978..>9581
                     /locus_tag="PKK81_08590"
     CDS             8978..>9581
                     /locus_tag="PKK81_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HNV32974.1"
                     /translation="MTMWPDGFTTRYRIAKDTLILDLGTRMRVLSSAPKGGGLRTTRY
                     IVNHQVPSNPVRQATHSKWEHPARYLKRIAVDLGVDPDCVGLMTAVPMKQVVTCREAQ
                     DGIWVECFATVGVTNAVRAGEPPPREEADSTVRRAGTINLMVVTNACLAVPALVCAVQ
                     VVTESKTGVLRDHAVPSWTGRPGATGTGTDAVVVACALRGS"
BASE COUNT         1730 a         2551 c         3179 g         1921 t
ORIGIN      
        1 ttcatggaat acggatcctt cgcctccctg cgtgaaggtg gcatggtgtc cgggaaacag
       61 ggcgcggtcg actacagctt ctcgctgtcc cgctgggaca cctcgagttt ttcggccgtc
      121 aattatcggc gtggcgccgg cgagcgagac tcctatcgta actggcaggg atcagggcga
      181 atcggtgtag accttccaaa ggacggccgt cttgatttca cgatgcgctg gatgaattcc
      241 gacgtgcaac tcgatagtgt atcggcgacc tctccaagcg acgtctatgg ctcaaaaaat
      301 cggagtcagg aatatgtgtt cagcggtagt tatgaacagc cgctcaccgg ctggtggtcc
      361 cagaagctca cgcttgcgcg tgcacaggag gcctcgcagt ttttgccagg cacgttgcag
      421 cgcagtctga tcaccggggc attcagcacg ccgttcggca cccccaatga aattcgtgtg
      481 ctctcgaatc ggttggagtg gcagcacaat ttccaaatca cgaagctgtt gttgttgagt
      541 gcggggtatc aatttcgcga acaacagggc gagaacgata cgggactcac caaccggatt
      601 ctgagttcca acgccgggtt cgcgcaggca caattcaatc tgtgggatcg cctgttcgcc
      661 accgcgggca ttcgccatga cagttacaac gtgttcggcg atgcgacgac ctatcggctg
      721 accggcgggt atctccacaa ggaaaccgac acgaaaatcc gaggcagcta ttccactggc
      781 ttccgggctc ccacgatgaa cgaattgtat tttcccaatt ttggcaattc ccaattgggc
      841 acagaaaaaa gccaaagtat ggatgtgggg atcgaccagt atttcttctc gaagcagctg
      901 aagttcacag gagggttttt ctggaaccgc taccggaatc tcatcaccac gacgtttgat
      961 ccgacgtttt gcgcgccgtt cagcacgttc gggttttgcc ctcagcagat cggtgaagcc
     1021 tcgacgaagg gctgggaggc tggactctcg tacacctatt ccagcgaccg tccgttcctc
     1081 aagggccttg tggtgcaggc gaactatacc aacaccatga ctcgggattt ggtcactcag
     1141 actcgtctac cgcgttgggc caccgaccag tggagtgcct cggtcagcta tcaacccatc
     1201 gatccgctct ggatcacgct gatcggacgg tacgtgggtt cccgatacaa taccaccggg
     1261 gatcggcagt cgctgccggc gttcgatgtg tggtcgttgg cagtcacgta cgatgtcacg
     1321 aagcagctcc aggcctatct ccgggctgag aatttgttta atgaaaagta tgaagaagtc
     1381 gccagcgccg gcacaccgat ccgctcgatt tttggcggtg tgcgggtcac gtttggcgga
     1441 aagtcgtgag gaagagatga gcgccgtact gtaatgtcac gtcctcgtct cgtcatcgcc
     1501 ggcacccaca gtggtgccgg caaaaccacg gtcaccctgg cgctcatggc nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggccgtaca ggttcgtgga gtgctgttca
     1681 atcgcgtgcg gagtgagggc cattaccagt tattgcggca ggcggtagag tcggagacgg
     1741 atctggcggt tgtgggatac ctgcagcccg atgcttcggt gaccattcag gatcgccatc
     1801 tcgggctgag gactgccatc gagcaggggc agggcgatct gtatgagcga ttggcgagag
     1861 ccgctgcaca gacgatcgat ttggatcggg tcggggccat ggctcgatca gccggggaat
     1921 gtccgggaga agatctcccg atcacgaggc cggcggcaac gcaacaaccg gttcgagtcg
     1981 gcctggccta cgatgcggcc ttttgttttt actatcagga taatctggag ttacttgaat
     2041 cggcaggcgc ggagatcgta aggttttcgc ccttgcacga tgacgagttg ccggtcgcag
     2101 atctgctgta tttcggcgga ggctaccccg aactgtatgg cgagacattg gcggccaata
     2161 tgtccatgcg ttcggccgtg cagacatttg cccggcgtgg tggggctgtg tatgccgaat
     2221 gtggcgggct gatgtacttg acggaggcca ttcgtgacgg ggccggcgct cgacatgaaa
     2281 tggtcggggt gtttccggcg gatgcggtca tgcggaaaaa cgggatgacg ttaggttatc
     2341 gaaccgtgga gattgctgaa tcctgcatgc tgggcggagc cgggacagtg gtgcgcgggc
     2401 atgagtttca ctattcgatg ctcgaaccaa ccggcgaact ccatcatgcc tgtaccttgt
     2461 cggatgcagc agggaaaccc gtcgggcagg acgggttgac gatggggaat acccttgcgt
     2521 tgtacagcca cctgcatttc ggcagtcatc cggctgtcgt ggctgatctg ctggggactg
     2581 cccgtcgact gcgcggggcg caggcctctg ccacgaaagg atgagatggc ggaacaggac
     2641 gagcatacgg cgaagatgca acgcttgaag gcctccgtgg atcggcggat cgaagcggcc
     2701 caggacgaga aggggctgct gatcgtctac accggcgcgg gaaagggaaa aacgaccgcc
     2761 gcgctcggga tggccctccg ttgcctcggc cacggcatga aggtggcgat cgtgcaattc
     2821 atcaaggggg cgatcgacac ggcagaggag cgcatcctga aatcgttcgg cgaccgcgtg
     2881 acgttcctcc gtatgggcga agggtatacc tgggagacgc aggatcggga gcgcgatacg
     2941 agccatgcgc aagcggcctg ggcgaaggct tgcgagttta tgcgtgatcc gtcctatgcg
     3001 atggtgatcc tggatgaatt caatattgcg ctgcaccacg gctatgtggc ggtgactgaa
     3061 gttctgccgc gtttgcagca gcggccggcg atgcaacatg tcgtgatcac cggcagaggg
     3121 gctccggagg aattgatcga ggctgcagat ctggtgacgg agatgaagca ggtgaaacat
     3181 ccgttccgaa aaggcatcaa ggcgcaggcg ggggtggagt tttgagaggg ccggtgacca
     3241 agacctgtga gcattgccgg aagacgttcg agtgtgtcgg gtatcaatgc tggtgcggga
     3301 agatcggtat taccgaggag cagatggatt ggattgcggc gcgttttaag gactgccttt
     3361 gccccgtctg tttgcagcag gtggcggatg gaatactcgg accgcaggct tcgaatggaa
     3421 tgcctcatgg cacgtgacaa acgagagcgg ttgactcgca caacggccga gtcttcagaa
     3481 ccgttttcca aggctgaacg agaggcggtc tatcgggcta ttttcgagcg ccgggacgtg
     3541 cgcagaaact ttctgccgac gccgattccc gacgaagtgc tcgcacgctt gctgaacgcc
     3601 gcgcatcatg ccggatcggt ggggttcatg cagccctggg actttgtggt ggtccgtaca
     3661 gatgagacaa agcgtgcggt gaagcagctg tttatggaca cgaatgcggc tgcggcgctc
     3721 cggtacaaaa cacctcgtgc ggatctgtat cgaacgctca agctggaagg tattgaagaa
     3781 tcgtcgctca atctctgtgt gacctgcagc cgccaacgag gtggcccgca cgtgttaggc
     3841 cgttccaccg tgcgggatac agatctctac agcacctgtt gtgccattca gaatctctgg
     3901 ctggcggcac gcgcggaagg tatcggggtt gggtgggtca gtattttgaa tcacgcggcg
     3961 ctgaaacatg tgctgggtat tccgaggccg gtcaaggtgc tggcctacct ctgtctcgga
     4021 tatgtgtcgg agtttgcgaa gaagccggac cttgaagccg ccgggtggcg agccaggctt
     4081 tctgtgcaag atttggtgca ctatgaggca tggggtaaga gagtcgaagg gaaggagaga
     4141 ctaccgggat gcgaatcacc aaggtctata caagaacggg cgacgcgggc caaacgaggt
     4201 tagccggcgg acaacaggtc tggaaagact gtctgcgggt cgaggcctac ggcacagtcg
     4261 atgagttgaa tgcgtccgtc ggattggtgc gggcgatgaa cgccgacgcc gagaatggct
     4321 ctgccgcgtc gacgcaattg gaggcggatt tgcgctgggt gcagaacaag ctcttcgatg
     4381 tcgggagtat tctggccacg gcgccgggtc agacctttcc caacatgccg gaggtgacgg
     4441 ccaaggatgt gacgaaactc gaagcgatga ttgatcgatg ccaggaggag cttgccccgc
     4501 tgaaggagtt cattctaccc ggtggcggta aggtctcggc gacgctccac caagcgcgaa
     4561 cgatctgccg ccgcgcggaa cgaatctgta tccggttgag ccgcgaggaa ccggtggcag
     4621 ccgagctcaa taagtatctg aaccggttga gcgacgcgct gttcgtcctg gcccgctggg
     4681 tgtcgaagac ccagggcgaa ccggaatttt tgtggcaacg cgaatccggc gcaaccgccg
     4741 agtaagccga tgaagaagca cccggccaca ggatcccatc tcattctggt gcttggcggc
     4801 gccgcctcgg gaaaaagtca ggcagccctc gatcgggcgg gccgtcgagg cccgaaggcc
     4861 tttgtcgcga ccgggcaggc gctggacgat gaaatgaagg cgcgtattgc gcggcaccgc
     4921 gagacccgtc ccaccggctg gatcactgcc gaggtgccgc gtgacctggc tgcctggttt
     4981 cgaacggaag gacaggccta tcgtaccgtc gtggtggatt gtctgacgct ctggttgagc
     5041 aacgtgtgcg ggcgtcgcat tgccgggcag gagatggtcc ctcgtgtgga agcagtgctg
     5101 caggctattc gcggcaccca ggcgcgggtc gtgttggtga gcaacgaatt gggcttcggg
     5161 ctggtaccga cgagtcgcag tgcgcgcgcg tttcgcgatg tcgccggtcg ggtcaaccaa
     5221 cagatcgcgg cagcggccga cgaagtctac tttgtcgtca gcggccagtc gctcaaactc
     5281 aaatgacagg ggagaagacc cgatgacgct ccaggaagtg ttgacacgca ttcaaccggt
     5341 ggatcaggcg atcgcagcgc agacgcaagc gcgcctggat cggttgacca aacctctcgg
     5401 tagtctgggg cggctggaag aaaccgcggt gcgttatgcc acgatcacgg gcgaagtgaa
     5461 accggctgtg ccgcgcggag tcgtgtttac gtttgcggcc gaccatggtg tggcgactga
     5521 aggggtgagc gcctatccgc gcgaagtcac gccgcaaatg gtgctgaatt tcctgcgtgg
     5581 cggcgccggc gtgaatgtgc tcgcgcgtca cgccggagtc gaggtgcggg tggtggatat
     5641 tggagtcgcc tacgactttg ggtcggtctc cggcctgatt cacaagaagg tgatggcggg
     5701 aaccaacaat ctcctggtgg aatccgccat gagtcgtgac caggcggtgc aagcattgca
     5761 ggtcgggatc gaggtggcta cggaggctgc gcgtgagggg atcggtttga tcgggaccgg
     5821 cgagatgggc atcggcaaca cgacgccgag tgcagcgatc acagcagtca tgaccgggcg
     5881 gccggtggct gaggtcacgg gtcgaggaac cggcatcgat gaggcgggcc atgcccgcaa
     5941 agtcggcgtc attgagcagg cgctcgaccg gcatcgtccg aaccgggccg acgccatcga
     6001 tgtgctcgcg aaggtcggcg gtctggagat tgccggtttg gccgggctga tcctgggtgc
     6061 ggcggcagca cgagtccctg tggtgttgga tgggttcatt gccggcgctg cggcgttgat
     6121 cgcggtgggg ttgcagccgc tctgtcggga ctatctgatc gcctcgcacc gttcggtgga
     6181 acgtggtcat caggcgattc tggagcatct gcgcttgaag ccgctgctcg atctcgatct
     6241 gcggttaggc gaagggactg gagcctgcct cgggatgagc ctcgtgcagg cgtcgatcaa
     6301 gattctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngacg ggctcgcaga
     6421 caccctggat gggttggccg gcggacgcac ggcggccgat cggcttcgca tcatgcgcga
     6481 tccgagcgtc ggggcactcg gcgcgaccgg cctttttctc tcgctgctgt tgcggtatgc
     6541 cggattgctc gccctccctc aaccggttcg tcttcccgtg ctgctctgta tgccggcgct
     6601 gggccgttgg gcgatggtct cagtcgcctg ggcgtctccc tatgccagga gcgaaggcgg
     6661 gctagcggcc gcgtttttga ctcatctctc ctggcagcat gtgttgcttt cgagtctcgt
     6721 gttggcctgt gcgctgatgg ccggaatggg agtgattgcg gcaacggcga ctatcgcatc
     6781 gggagcgttg gtggtggtcg tggttcggcg cggttgtcgg gcatggttcg gcggagtgac
     6841 gggagatctg ttgggtgcca cgaatgaatt gatcgaaatt ctcttcctct tgttgatccc
     6901 ggtgttggtg atggcgcgat gaccggcggc gagttggtac tggcggcggc gctggatgtc
     6961 gtggcgggag atccccgttg gttgccccat ccggtgcggg gcatgggcgt ggtcatttcc
     7021 tggttcgatg atcaggtcag aacattctgc cggagtgatc aatccttaca aatcgccggg
     7081 gcctgtctcg cgctggggtt accggcggcg gtctatgcgg cggccacctg gctgattgcg
     7141 caggcggctg cattttctcc gctcctcgga caactggtcg gcatcggcct ggcctatacc
     7201 acactggcgg gccgggacct cttcgatcat gtccaggcag tcagtcgtga gctgcagcag
     7261 gggaatcttg ccggagcgcg tgaggccgtg gccaggatcg tcggccggga cagcgccacg
     7321 ttggaggaac cggaaatcgt gcgcgccacg gtggagacga tcgccgaaag caccgccgac
     7381 ggcattattg cgccgctcgt ctacctctcc cttggcggcg cgccgctcgc gctggcctac
     7441 aaggcggtga atacgctcga ttcgatggtg ggacaccacg atgagcggta tgaacatttc
     7501 ggctgggcct ctgcccgatt ggacgatgtg atgaattggg tgcctgcccg gctgactggc
     7561 ggcttcatcg cgctggcttc gggtctggcg accgctcagt ggcatcgtgt gcaggacagt
     7621 tggtacatcc tgcatcggga cggcgacaaa catcccagcc cgaacagcgg catgccggaa
     7681 gcggccatgg ccggtggatt gggcgtgcaa ctcggcgggc ggaattatta cgacggtgtg
     7741 gctcacgagg gtgaattgat cggtgatgcc ggcaccgcgt tgacgcccgg gcacatcgat
     7801 caggccactc gcattatggt ggtcgctgcc ggactggggc tcttctttgc actgctctcc
     7861 ctatgggtat catgacgcgc atcggtcatg gcggggatgt gtatgcggcg gcccgtgagt
     7921 tagggcgaga tctccatcga ctcctggatt tcagcgccag catcaatccc ttgggaccgt
     7981 ctcccgccgc agtgcgaacc ctgcggacag ccgaggtgtt gctcggtcat tatccggacc
     8041 cggcttgttg ggcactgcgg caggcgctgg ccgcgtattg gaaacgcccg agtgaggagt
     8101 ttctggtcgg caacggatcg accgagttga tccatctcct tcccaggaca ttggggatca
     8161 gacatctcct gctgatcggc ccgacctttt ccgaatatgc tgaagcgatg acacgcgccg
     8221 ggggacgggt caccatgctg atggcggatc gtgccgacgg atatcgtcct ccgctggagt
     8281 cggtcctcgc aacgttgcag aaggggcggc agtctgcgag cggcgcctat cccattgatg
     8341 cagtactcct ctgcaatccc aatagcccga ccggtcaggc ctgcgaggct gccgcggtaa
     8401 ggaagtgtgc gcgtctggtg gctcgccggg gactctggtg tatcgtggat gaatcctttg
     8461 cggaatatgc gaatcatgcg tcgatcttgt cagagcctct tcccccacgg accgtcgtgc
     8521 tgcgcagctt taccaagttt tatgggttgc ccggactgcg tgtgggctat gccacggcga
     8581 agcctgctgt gattgccaga atcgcggcgc agcagccgcc ctggtcggtg aacatgttgg
     8641 cgcagcgaac tgcggtcgcc gccttgaaag atgtgcgaca cgccgggcga agccttcgtt
     8701 tcatggagcg ggagcgaacc cgcgttcaga aggcactgac ccggttgccc ggttgcacgg
     8761 tctttccctc tgccgccaat tttctgctga tagaggtgcc ggtcgggcag aaagcgaccg
     8821 ccgtggtggc cgcgcttcgc cggcagggac tcctgataag ggattgttcg caagtgcctg
     8881 gattgaatga ccgttcggtg cgcgtcgcag tgcgtcccag ggccgacaat gatcgcctcc
     8941 tgcgaaccct gtcggttctc cttcacaagg gtggcgcatg acgatgtggc cggatggttt
     9001 caccactcgc taccgaattg ccaaggacac gctcatcctc gatctgggaa cccgcatgcg
     9061 ggtgttgtcg tccgctccaa aggggggagg gcttcgaacg acgcgctaca tagtgaatca
     9121 tcaggtgccc tccaatcctg ttcggcaggc gactcactcg aagtgggaac atcctgcccg
     9181 ttacctcaag cgaatcgcgg tggatttggg agtggatcct gattgtgtcg gcctgatgac
     9241 ggcagttccg atgaaacagg ttgtgacgtg ccgtgaggcg caggatggaa tctgggtcga
     9301 gtgttttgcc accgtcggtg tcaccaacgc ggtgcgggca ggtgagccgc cgccgcgaga
     9361 agaagctgac agcacggtcc gtcgagccgg gacgatcaat ctgatggtgg tcaccaatgc
     9421 ctgcctcgcc gtgccggccc tggtctgtgc ggtgcaggtg gtgacggaga gcaagaccgg
     9481 agtgctgcgt gaccatgcgg tgcccagttg gaccggtcgt cccggcgcga ccggcacggg
     9541 gacggacgcc gtggttgtcg cctgtgccct gcgggggagc g
//
LOCUS       DAOQRM010000094         9496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6599_length_9496_cov_13.336511, whole genome shotgun sequence.
ACCESSION   DAOQRM010000094 DAOQRM010000000
VERSION     DAOQRM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6599_length_9496_cov_13.336511"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /gene="nusB"
                     /locus_tag="PKK81_08595"
     CDS             <1..458
                     /gene="nusB"
                     /locus_tag="PKK81_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNV32975.1"
                     /translation="ARERAVQILFQYDVHGQAGPWLDDFWIQYPLAEELRVFAERLVA
                     GVRANKKELDALIGTYATNWKVSRMPIIDRNILRMGCFELLQVPEVPAKVTLNEAIEL
                     AKSFGDEEASKFVNGILDKVLGSDARLERKRAFPELPVVGSDSSVKGDA"
     gene            535..1869
                     /gene="purB"
                     /locus_tag="PKK81_08600"
     CDS             535..1869
                     /gene="purB"
                     /locus_tag="PKK81_08600"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNV32976.1"
                     /translation="MIDRYTRPRMSAIWDLQHKYEIWLEVELQACAAFERGGQIPRGT
                     SARIRKKAKIDVARIAEIEKVTKHDVIAFLESLMDSVGPEHRFLHMGLTSSDIVDTSL
                     AVQMTEALDLILEDLNELIAVLKKRALEHRHTVMVGRSHGIHGEPVSFGFKLAIWYEE
                     ACRHRTRLEAARKDIAVGKLSGAMGTFAHQGPEIEEYVCAKMGLASDPVSNQIVQRDR
                     HAAYASALALLAATIEKIATEIRHLQRTEVLEAEEYFSAGQKGSSAMPHKRNPIASEN
                     LCGLARLVRGNSLAALENVALWHERDISHSSVERVIMPDSTILVDYMLARVTDLVKNL
                     IVYPERMQRNLDLTGGLVYSQRLLLALIDKGAQRKESYEAVQRNAMTAWKGGVGFQEL
                     VARDPFITTHLKPHEITACFDPAYYLRHLDQVFRRVFGASGASSAKRRRKRA"
     gene            1866..2660
                     /locus_tag="PKK81_08605"
     CDS             1866..2660
                     /locus_tag="PKK81_08605"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNV32977.1"
                     /translation="MSLRSTARVGLLAGLLVLAAVWPVSAADREKLLTEPGVYGTFAT
                     FQMDHDWWDLPGESRVISVAEVKGLVEQWSGKILVESYLLRGLSDHADLMFRVHARTL
                     SDTQQFLSAFMGTRLGRHLTSGGLLHGVSKKPTYVAGFPESMKTELQVNGESGSRPYA
                     IVIPIKKDAEWWGLDQEARTALMQEHTQAALPYLKTVKRKLYHSTGLDDVDFITYFET
                     EKLEDFHNLVRALQQVKEFRHNRRFGHPTLLGTMSPLDEILEKFAQ"
     gene            2676..3389
                     /locus_tag="PKK81_08610"
     CDS             2676..3389
                     /locus_tag="PKK81_08610"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNV32978.1"
                     /translation="MTVEPAGTMIYEGKAKKIFTTDRPDQVLQYFKDDATAFNAQKRG
                     TIVDKGVVNNKVSERLFRLLEQHGISTHFIERVSDREMLTKRVTIVPVEVVVRNVVAG
                     SLAKRLGLKEGAAIDPAIIEFYYKDDALGDPLVNDDHLRLLNVATPAVLREMRELGHK
                     INAVLLPFFKERRMMLVDFKLEFGVFHNRLILADEISPDTCRFWDQTTKESMDKDRFR
                     KDLGKIEEAYQEVLKRVCE"
     gene            3397..3624
                     /locus_tag="PKK81_08615"
     CDS             3397..3624
                     /locus_tag="PKK81_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32979.1"
                     /translation="MNRFLQLFLNYGLVAAILVWAATVAMMAYHLKESPWRWAFVLMS
                     LAGLGTIGVIFWIRKYVQRVCKAQQEVGKAQ"
     gene            3621..3860
                     /gene="purS"
                     /locus_tag="PKK81_08620"
     CDS             3621..3860
                     /gene="purS"
                     /locus_tag="PKK81_08620"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNV32980.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAGNVRVGKYMEV
                     DVNEADRAKADAQVKQMCEKLLANTVIEDYRYELG"
     gene            3962..4684
                     /gene="purQ"
                     /locus_tag="PKK81_08625"
     CDS             3962..4684
                     /gene="purQ"
                     /locus_tag="PKK81_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNV32981.1"
                     /translation="MKIGVVVFPGSNCDHDCQYIFKDVLGQYVEMIWHKETLLAGLDA
                     VVLPGGFSYGDYLRTGAIARFSPVMGAVKAFANKGGLVIGICNGFQILLEAGLLPGVM
                     LRNTSLNFICKDVYVKVENAATRFTNQCESGQVLKIPIAHADGNYYTDPVTLGGIKAN
                     AQVIFRYCTADGKVTPDANPNGSLDNIAGIMNADGNVLGMMPHPERSAEAILGNEDGR
                     LIFQSMLSSFGKPELQSKLIGV"
     gene            4753..6999
                     /gene="purL"
                     /locus_tag="PKK81_08630"
     CDS             4753..6999
                     /gene="purL"
                     /locus_tag="PKK81_08630"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNV32982.1"
                     /translation="MNALVITKAILEQHKLSDDEYKKILEILGREPNWTELGMFSAMW
                     SEHCSYKSSRIHLKKLPTTGPRVVQGPGENAGAVDIGDGLCAVFKMESHNHPSFIEPY
                     QGAATGVGGILRDIFTMGARPIALLNSLRFGGLDNANTRHLLKGVVSGIAGYGNCMGV
                     PTVGGEIVFNDIYALNPLVNVFCLGIAKKDKIFLGKAAGVGNPVIYFGSKTGRDGIHG
                     ATMASDSFDEQAAQKRPTVQVGDPFTEKLLLEACLELMAGDCLVGIQDMGAAGLTSSA
                     CEMASREGTGVELELADVPRREPGMTPYEIMLSESQERMLMVVKAGREDEVIKICRKW
                     DLDVAVIGRVTDTGKVVLKENGQMVAEIPAKALADDGPRYDRPSAPPAYQEMLQALNH
                     DALPDVKDVNAALLALLDSPTIASKRWVYEQYDHMVRTNTMVRPGSDAAVVRVKGTNK
                     ALALSVDCNGRYCLLNPYEGAKIAIAECARNLACSGAQPIGVTDCLNFGNPQRPEVMW
                     QFVLAIEGLKDACEALNVPIVSGNVSLYNETNDLSIYPTPMIGMVGLIEEADRAVTQW
                     FKESGDVIILLGQTREDLGGTEYLKVLHHREQGSPPLLSLETEKAVQACTIRLIRDGL
                     VQSAHDCSDGGLAVALTECCISGSAEQLGAVVQLPLDGLRRDALLFGESQSRILLSVK
                     AELAERVLNAAWEAGVHAARIGTVGGARLVIQVDGDQRAAGCKIDLELTALYAHWGLA
                     IPRALGQD"
     gene            7012..>8339
                     /gene="purF"
                     /locus_tag="PKK81_08635"
     CDS             7012..>8339
                     /gene="purF"
                     /locus_tag="PKK81_08635"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNV32983.1"
                     /translation="MANELPLISPDKFHDECAVFGIYGHKEAANLAYLGLYALQHRGQ
                     EASGIVSNDGEQFYVEKGQGLVADIFSQQALARLPGTMAIGHNRYSTAGGAGLKNVQP
                     LSVNFAFGNLAVAHNGNLINATMLRSELEAYGAIFQSTSDTEVIIHLIAHSRADTLLD
                     RVIDSLTQVRGAFSVVLMTDQGIVAARDPHGFRPLCLGRFRDAWIVASESCAFDLLDA
                     EYVREIEPGELVVLDHQGVTSYKPFAQTKPAMCVFEYVYFARPDSRIFGGKAVYSIRK
                     AFGRQLAQESRVDADIVIPVPDSGVPAALGYSEGSGFPFETGLIRNHYVGRTFIEPEQ
                     SIRHFGVKVKLNAVPEVLEGKRVVVVDDSLVRGTTSRKIVKMLRHAGAKEVHMRISSP
                     PIVSPCFYGIDTPTKKELIASSHTTEEIRKYITADSLAYLSLDGMVKAAPG"
     assembly_gap    8340..8439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8858..9175
                     /locus_tag="PKK81_08640"
     CDS             8858..9175
                     /locus_tag="PKK81_08640"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNV32984.1"
                     /translation="MPATVHLWSEPQLELSEIFPRQDEQVHSTVLDGESVLLNLNSGR
                     YYTLNVVGSTIWNLCRGEQSLRQIHSAICARFDVSEQQAQDDMLALIVLLEQEGLLHT
                     ERR"
BASE COUNT         2028 a         2370 c         2949 g         2049 t
ORIGIN      
        1 aagcccgtga acgggcggta cagattctct ttcaatacga cgttcatggc caggcgggcc
       61 cctggctgga cgatttctgg attcagtatc ccttggccga ggagttgcgg gtattcgcgg
      121 agcgattggt cgccggcgtc cgggccaaca agaaggaact ggatgcgctg atcgggacct
      181 atgcgaccaa ttggaaggtc agccggatgc cgatcatcga ccgcaatatt ctccgcatgg
      241 gttgttttga attgctgcag gtgcccgagg tgccggccaa ggtgacgctg aacgaagcga
      301 tcgagctggc caagagtttc ggcgacgagg aggcttccaa gttcgtgaac ggcattctcg
      361 acaaggtgct cgggtcggat gcacggttgg aacggaagcg cgcgtttccc gaattgccgg
      421 tggtgggcag cgacagcagc gtaaagggtg acgcgtgaag cgtatctcgt ggggtgcccc
      481 cctcggattt tctgaacgaa cgacgcttca cgagcgacga ataacgagga agagatgatt
      541 gatcgataca cccgtccgcg gatgagtgcc atctgggacc ttcaacacaa gtatgaaatc
      601 tggctggaag tggagttgca ggcctgcgct gcgtttgagc gtggcgggca gattccgcgc
      661 gggaccagcg cccggattcg caagaaagcc aagatcgatg tggcgcgcat tgccgaaatc
      721 gaaaaggtca caaagcacga tgtgatcgcc ttcctcgaat cactgatgga ttccgtcgga
      781 cctgagcacc ggttcctgca tatggggctc acctcatcgg acatcgtcga tacgtcgctg
      841 gccgtgcaga tgaccgaagc gctcgatctg attctcgagg atctcaacga gttgattgct
      901 gtgctgaaga aacgggcact cgagcatcgc catacggtga tggtaggccg gtcgcacggc
      961 attcatggcg agccggtgtc gttcgggttc aagttggcga tttggtatga ggaggcctgc
     1021 cggcaccgga cgcgcctcga agcggcgcgc aaggatatcg ccgtaggaaa gctctcggga
     1081 gccatgggca cctttgcgca tcaaggccct gaaattgaag aatatgtctg cgccaagatg
     1141 gggctcgcgt cggatccggt gtccaaccag atcgtgcagc gagaccggca tgcggcctat
     1201 gcctccgcgc ttgcgctact ggccgcgacc attgaaaaaa tcgccaccga aattcgtcac
     1261 ctgcagcgca cggaggtgct ggaggcggaa gaatatttct ccgccggaca gaagggctca
     1321 tcggcgatgc cccacaagcg caatccgatt gcgtcggaaa atctgtgcgg cctggcgcgc
     1381 ctggtacgcg gcaatagcct ggccgcgctg gagaacgtgg ccctgtggca tgagcgtgat
     1441 atcagccact cctcggtgga gcgggtgatc atgccggaca gcaccattct ggtcgattac
     1501 atgttggcac gcgtgaccga cctggtgaag aatctgattg tgtatcccga gcggatgcaa
     1561 cgcaacctcg atctgaccgg aggcctggtc tattcgcagc gactgctgct ggccttgatc
     1621 gataagggcg cccagcgcaa agagtcgtac gaagcggtcc agcgcaacgc gatgacggcc
     1681 tggaaaggtg gagtcgggtt tcaggaactg gtcgctcgcg atccgttcat cacgacccac
     1741 ttgaagccgc acgaaatcac cgcctgtttc gatccggcct attaccttcg gcatctggac
     1801 caagtcttcc gccgggtgtt tggggcgagc ggtgcttcgt cggcaaaacg acggaggaaa
     1861 cgggcatgag cctgcggtcg acagcacgag tcgggctctt ggccggtttg ctggtgctgg
     1921 ccgcggtttg gccggtttcc gcagctgatc gcgagaagct tctgacagag ccgggggtat
     1981 acggcacatt tgcgaccttc cagatggacc atgattggtg ggacctcccg ggcgagtcgc
     2041 gcgtcatttc ggtggcggag gttaagggat tagtcgagca gtggtccggc aagattttgg
     2101 tcgagtccta tctgctccgg gggttatccg accatgccga tctcatgttt cgtgtgcatg
     2161 cccggacgtt gtcggacaca cagcagtttc tctcggcatt catgggcact cgattaggac
     2221 gccatctcac ctcaggcgga cttcttcatg gcgtgagcaa gaagccgacc tacgttgccg
     2281 gcttccctga atccatgaag acagagttac aggtgaacgg cgaatccggt tccaggccgt
     2341 acgcaattgt gatccctatc aagaaggacg cggaatggtg gggactggac caggaagcac
     2401 ggaccgccct gatgcaagag catacgcagg cggcgcttcc ctatctgaag acggtgaaac
     2461 gaaaactgta tcattcaacg ggtctcgacg acgtcgattt catcacctat tttgaaacag
     2521 agaagttgga ggactttcac aatctggtgc gggcgctgca gcaggtgaag gaattccgcc
     2581 acaatcgacg cttcggacat ccgaccctgc tgggcacgat gagtccgctg gatgagatcc
     2641 tggaaaagtt cgcacagtag tcgaaggaga gagggatgac cgtcgaacca gcaggcacga
     2701 tgatctatga gggcaaggcc aagaagattt tcacgactga tcggcccgac caggtgttgc
     2761 agtatttcaa ggatgacgcc acggccttca atgcgcagaa gcgcgggact atcgtcgata
     2821 aaggggtggt caacaataag gtgtcggagc ggctgttccg gcttctcgaa cagcacggga
     2881 tttcgacgca cttcatcgag cgcgtcagcg accgtgagat gctcaccaag cgagtgacga
     2941 ttgtgccggt cgaagtggtg gtccggaacg tcgttgcggg gagtcttgcc aagcggctgg
     3001 gcctcaaaga aggcgcggcc atcgacccgg cgatcatcga gttttactat aaggacgatg
     3061 cgttggggga tccgctggtg aacgacgatc acctgcgcct attgaatgtg gcgactccgg
     3121 ctgtgttacg ggagatgcgc gagctgggcc acaagatcaa tgccgtgttg ctgccgtttt
     3181 tcaaagaacg ccggatgatg ctcgtcgatt tcaagttgga gttcggcgtc tttcacaatc
     3241 gactcatcct cgccgacgaa atttcccccg atacctgccg gttctgggac cagacgacca
     3301 aagagtcgat ggacaaggac cggttccgga aggatttggg caaaatcgaa gaggcgtatc
     3361 aggaagtgtt gaagcgggtg tgcgaatagg ggcggcatga accggtttct ccagttgttt
     3421 ttgaactatg gtctggtggc ggcgattctg gtgtgggcgg ccacggtggc gatgatggcc
     3481 tatcacctca aggaatcccc ctggcgctgg gcgtttgtcc tgatgtcgct ggcagggctc
     3541 gggaccatcg gcgtgatctt ttggatcagg aagtacgtgc agagggtctg caaggcacag
     3601 caggaagtgg gaaaagcaca atgaaagcca agattcatgt gacgctgaag cagggaattc
     3661 tcgatccgca ggggaaggcg atcgaacatg cgctggactc cctcgggttt aagaatgcgg
     3721 gaaacgtgcg ggtcggcaag tacatggaag tcgacgtgaa cgaagcggac cgcgccaagg
     3781 cggatgccca ggtgaagcag atgtgtgaaa aattgctggc gaatacggtg atcgaggatt
     3841 atcggtacga actcggatag gagcagatgg cttatagcct atagctgatg gcagaagccg
     3901 agacctcgtt cctgtctcgt atcatacgcc atttgccata ggccataagc tctttagaat
     3961 tatgaaaatc ggcgtggtgg tatttcccgg cagtaattgc gatcacgact gccagtatat
     4021 cttcaaggat gtgctcggcc agtatgtgga gatgatctgg cataaggaaa ctctgctcgc
     4081 ggggctcgat gcggtcgtgc tgccgggcgg gttttcctac ggggattatt tgcggaccgg
     4141 cgccatcgcg cggttttctc cggtgatggg agccgtgaag gcctttgcga ataagggtgg
     4201 cctggtcatc ggcatctgca acgggtttca gattctgctg gaggctggtc tcctgccagg
     4261 tgtgatgctg cgcaatactt cgctcaattt tatctgcaaa gacgtctacg tgaaggtgga
     4321 aaatgcggcg acacggttta cgaatcaatg cgagtccggc caggtgctga agattcccat
     4381 cgcgcatgcc gacgggaact attacaccga cccggtcacg ctcggcggca tcaaagccaa
     4441 tgcacaagtg atctttcgct actgcacggc agacggcaag gtgacgcccg atgcgaaccc
     4501 gaacgggtcg ctggataaca tcgccggaat catgaatgcg gatggaaatg tgctgggcat
     4561 gatgccgcat cccgaacgca gtgcagaagc gatattgggc aatgaagatg ggcggctgat
     4621 ctttcagtcg atgttgagct cgttcggcaa gcctgaattg caatccaagt tgatcggagt
     4681 atgacggccg gttcgcgtga gcagcggtgc cggaactgtc ggtaaccccc tacgattgag
     4741 aatgtgtcat tcatgaacgc actggtcatt accaaagcca tccttgagca gcataagctg
     4801 agcgatgacg aatataagaa gatcctggag atcttggggc gagaacccaa ctggaccgaa
     4861 ctgggcatgt tctcggccat gtggagcgaa cattgttcct acaagagctc ccgcatccac
     4921 ttgaagaagt tgccgacgac ggggccgcgc gtggttcagg ggcccggcga aaatgccggc
     4981 gcggtggata tcggggatgg tctgtgcgcg gtgttcaaga tggagtctca caaccatcct
     5041 tctttcatcg agccctacca gggtgcggcg acgggggtgg gtggaattct acgcgatatt
     5101 tttacgatgg gcgctcggcc gattgcgttg ttgaactcgc tgcgattcgg cggattggac
     5161 aatgcgaata cccgccatct gctcaagggt gttgtgtcgg gtattgccgg ttacggcaac
     5221 tgcatgggtg tacccactgt cggcggggag attgtcttca acgacatcta tgccctgaat
     5281 cctttggtga atgtgttctg tctgggaatt gccaagaagg ataagatttt tttgggtaag
     5341 gcggccggcg tcgggaaccc ggtgatctat ttcggctcga agaccgggcg tgacgggatt
     5401 catggcgcga cgatggcctc ggactcgttc gatgagcaag cggctcagaa gcggccgacg
     5461 gtgcaggtcg gtgacccgtt cactgagaag ctgttgctcg aagcctgttt ggaactgatg
     5521 gccggtgatt gcctggtcgg cattcaagac atgggtgctg ccggtttgac gagttcggcc
     5581 tgcgaaatgg cctcgcgaga aggcacgggt gtagagttgg agttggctga tgtgccgcgc
     5641 cgtgaaccgg gcatgacgcc ctacgagatc atgctctcgg aatcccagga acggatgttg
     5701 atggtggtga aggccggtcg tgaagacgag gtgatcaaga tttgccgtaa gtgggatctg
     5761 gatgtggccg tgatcggccg ggtgaccgat acgggcaagg tcgtcctcaa agaaaacggc
     5821 cagatggtgg cggagattcc ggccaaggct ctggccgacg acggtccgcg atatgaccgg
     5881 cccagtgcgc cgcctgccta tcaagaaatg ttgcaggcgc tgaatcacga tgccttgccg
     5941 gacgtcaaag acgttaatgc cgcattgctc gcactcctgg attcgccgac tattgccagt
     6001 aagcgatggg tctatgagca gtatgaccac atggtgagaa caaatacgat ggtgcgtccc
     6061 gggtcggatg cggcggtggt gcgggtgaag gggacgaata aggcgctggc gttgtccgtc
     6121 gattgcaacg ggcgctattg tctcttgaat ccttatgagg gcgccaagat tgccattgcg
     6181 gaatgcgcgc gaaacctggc ctgctcggga gctcagccga tcggcgtgac tgactgcctg
     6241 aatttcggca atccgcagcg ccccgaggtc atgtggcaat tcgtacttgc gattgaaggg
     6301 ctcaaggatg cttgcgaggc gttgaatgtg cccatcgtga gcggcaatgt gagtttgtac
     6361 aatgagacga atgacctgtc gatttacccc acgccgatga tcgggatggt cgggttgatc
     6421 gaagaggccg atcgtgccgt gacgcagtgg ttcaaggaaa gcggcgatgt gatcattctc
     6481 ctgggccaga cccgtgaaga tctcggtgga acggaatatc tgaaggtctt gcaccatcgg
     6541 gagcagggtt cgccgccttt gctgagtctg gaaactgaaa aagccgtgca ggcttgcacg
     6601 attcgactca tccgcgatgg attggtgcag tcggcccatg actgttccga tggtggattg
     6661 gcggtggcgc tgacggagtg ttgcatctcg ggttcagctg aacaactggg ggctgtggta
     6721 caattgccgc ttgatggtct tcgtcgagat gcgctgctct ttggggaaag ccaatcgcga
     6781 attctgctct ccgtgaaagc ggagctggcc gagcgggtct taaatgcggc ctgggaggcc
     6841 ggtgttcatg ccgctcggat cggtacagtc ggaggtgccc ggttggtgat tcaagtagac
     6901 ggtgatcagc gggcggctgg ctgcaaaatc gatctcgaat tgacggcact gtatgcccat
     6961 tggggattgg caatccctcg tgcattaggt caggactagg atacggacgt catggccaac
     7021 gaattaccgc tgatttctcc cgataagttc cacgacgaat gtgccgtgtt cggtatctac
     7081 ggtcataaag aggccgcgaa cctcgcctac ctcggtctct atgcgctgca acatcgcgga
     7141 caggaagcgt ccggtattgt ctccaacgac ggcgaacagt tttatgtcga aaagggtcag
     7201 ggccttgttg cagatatttt ttcgcaacag gccttggcac gtctgcccgg cacgatggcg
     7261 atcgggcaca accgctattc cactgcgggc ggggcaggcc tcaagaatgt gcagcccttg
     7321 agcgtcaatt ttgccttcgg taacctcgcc gtggcgcaca atgggaattt gatcaatgcc
     7381 accatgcttc gcagtgaact ggaagcctat ggcgctattt ttcaatcgac gtccgacacg
     7441 gaagtcatta tccatctcat cgcccattcg cgggcggata cgctgctgga ccgggtcatc
     7501 gattcgctca cccaggttcg cggcgcattt tcagtggttc tgatgaccga tcagggcatt
     7561 gtcgccgcgc gcgatcccca tggatttcgc cccctgtgcc tgggacggtt tcgtgacgcc
     7621 tggatagtgg cctccgaaag ttgcgcgttc gatctgttgg atgccgaata tgtaagggag
     7681 atcgaaccgg gagagttggt ggtactggat catcagggcg tcaccagtta taagccattc
     7741 gctcaaacca agccggccat gtgtgtgttt gaatatgtct actttgcccg tcctgatagc
     7801 cgcatttttg gaggcaaagc cgtctattcc atccggaagg cattcggccg gcaattggcg
     7861 caagaatcgc gggttgatgc ggacatcgtg attcccgtgc cggattccgg ggtgccggcg
     7921 gcgttgggtt attcggaagg gtcggggttt ccttttgaaa ccggactgat ccggaatcat
     7981 tatgtgggtc gcaccttcat cgagccggag cagtcaattc gtcacttcgg ggtgaaggtg
     8041 aagctcaatg ccgttcccga agtcctcgaa gggaaacgcg tggtcgtagt agacgattcg
     8101 ttggttcggg gaacgacgag ccgaaaaatc gtcaagatgc tgcgtcatgc cggagccaaa
     8161 gaagtgcata tgcggattag ttctccgccg atcgtgtcac cctgttttta tggcatcgat
     8221 accccgacca agaaggaatt gatcgcgtcc agtcacacca ccgaagaaat tcgcaaatac
     8281 atcaccgcgg atagcctcgc ctacttgagc ctggacggca tggtgaaggc ggctcccggn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aatccctggt ttcctcaagt
     8461 actcaatcat ctcgcatctc ctgagacctc ctctgcacat taccgttctc ttggggtcaa
     8521 ctggacctgg cgggcctggg cgaatcgagt ctttcctgtg cgtctgcgtg aatcaagctg
     8581 acaagttcgg ctttgaaacc cactagcggc cccatccgca cgcttttttt atccgttttt
     8641 cataaaaaca aaatccggat cccccaaagg gaggtggagc ccaatctcac tggggataga
     8701 gaaacgagcg tgatgacgat agtttcgccc ttgtattgca gtgatgtgca cggcttgctt
     8761 gtagagggac caagacttcg gggaaaaatc ggtttcagaa cgaatggtgg tatcggagca
     8821 gtcacatgtc ccagcaatct gaaccaggag catctatatg cctgccactg tgcatctgtg
     8881 gagtgagcct caactcgaat tgtccgaaat atttcccagg caggatgaac aggtccacag
     8941 cacggttctc gatggtgaaa gtgtgctgtt gaatctgaac tccggccgct attacacgct
     9001 caatgtcgtc ggatcaacca tttggaattt gtgcagaggg gagcagtcgt tgcggcagat
     9061 tcactcagca atctgtgcca ggtttgatgt gtctgaacag caggcgcagg acgatatgtt
     9121 ggcgttgatt gtgcttctgg agcaggaagg attacttcac acagaaagga ggtgagtgag
     9181 aatggagcaa cagataaaga ccgaatacgt caagccggcg ctcgtgaaac atgagttgct
     9241 gcgcgacgta acggccattc agttgtcccg cgtgctggat cgaacaccca tataacatgc
     9301 caggtgctct agtgagagcc atggagctgg ccggttaact agaccggcca gctcgcctca
     9361 catctctgcg gtagtgacga cgggcagaag gctgaaaagg agaagcggta atgttcatgg
     9421 cgtcgacaat gaaggaactt ctactctccg tccatggcat agagatgaaa ttcaccacca
     9481 attcgtccga gttggc
//
LOCUS       DAOQRM010000095         9334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6792_length_9334_cov_17.229982, whole genome shotgun sequence.
ACCESSION   DAOQRM010000095 DAOQRM010000000
VERSION     DAOQRM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6792_length_9334_cov_17.229982"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1305)
                     /gene="mnmA"
                     /locus_tag="PKK81_08645"
     CDS             complement(223..1305)
                     /gene="mnmA"
                     /locus_tag="PKK81_08645"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNV32985.1"
                     /translation="MTRQTVLLGMSGGVDSSVAASLLVRQGYDVHGVTLQVWEHEDDQ
                     VAVSKRWEERGCCKIGIAKYVAQRLRIPYEVVDRREVFQQGVIDDFVAGYASGTTPNP
                     CVRCNERVKLRSLYALAHERGMDYVATGHYARVQQIDRQWSLHRALDARKDQSYFLYR
                     INPAWLPHLLFPVGHMQKCDVWQEAESLGLPVEELKESQEICFVSHGDYRTFIEQEMP
                     EAKKPGPFVGVDGEVLGQHEGIAFYTPGQRRGLGIAVGQRLYVQKVVPESGQVVLCAE
                     DQLVQSECQVADLSLFDHALGRQPVEADVKIRYATPPCPATLLPSESGTLQVQFHQPQ
                     RALSPGQSAVFYDGDRVLGGGIIQRS"
     gene            complement(1338..1793)
                     /locus_tag="PKK81_08650"
     CDS             complement(1338..1793)
                     /locus_tag="PKK81_08650"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV32986.1"
                     /translation="MWGETKKQPVAEDDKFTFLGKGTSFKGIVTFDGTVRIDGRVEGE
                     VHTGGAVIVGESAVIKGVIAAGSVSISGRVKGSVTALQKVEIQKPGILIGDIQSPVIM
                     IEEGAHFHGMSNMGAEKWTEDESSEFGAPQDPGVRDLVAHRGKVKTQEP"
     gene            complement(1923..2411)
                     /locus_tag="PKK81_08655"
     CDS             complement(1923..2411)
                     /locus_tag="PKK81_08655"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV32987.1"
                     /translation="MAWINKDKQDGKRQAGQSEGTEMENLESTAPREGNEEINAFVGK
                     GVSFKGVISYNGTVRIDGNLDGEIHTEGVLLVGEDAVLTAKITAGTVVCKGKITGDIS
                     AREKVKLRAPAVVNAGVKAPLLAMEEGVVFNGTLEMSQSGTREPQGQGSTRGQGVKLV
                     NG"
     gene            complement(2580..3428)
                     /locus_tag="PKK81_08660"
     CDS             complement(2580..3428)
                     /locus_tag="PKK81_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNV32988.1"
                     /translation="MEKKALGRGLDALLPTGRTTAEPERGDVQELRLEAIVPNRFQPR
                     QQFSEVELAELTASLKQNGLLQPILVRRKGDGIYELIAGERRLRAAKLAGMQKIPALV
                     RNVSDQESMVLALVENLQRDDLNPMETARAYQRMLNEFGLTQEAIAQKVACDRSSVAN
                     LLRLMSLPSEVQHMIESDQLSTGHAKVILGLMTPAAQVSLATQIVSGQLSVREAERLV
                     QEHAEARKPGKRPVRAPLRSDLEERLQKRLGTRVDVQKGRRGGKIVIHYFSPEELDGV
                     VERLLN"
     gene            complement(3406..4182)
                     /locus_tag="PKK81_08665"
     CDS             complement(3406..4182)
                     /locus_tag="PKK81_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNV32989.1"
                     /translation="MARIIAVANQKGGVGKTTTSVNLAAALAIEGGSVLLVDIDPQGN
                     ATSGLGVDAMSLTKTIYNALISKESIESLAMQTGVNGLSIVPANSHLAGAEVELVNME
                     DREQRLKEALAEVSDRYGTILLDCPPALGLLTINAMVAAHSVLIPVQCEYYAMEGLGR
                     LMESIQRLRQSLNPGLEIEGIVLTMYDARNSLARQVVEQIRGHFGASVYQTMIPRNVT
                     LAEAPSYGRPALLYNMASAGAQAYLSLAKEFVVHGEKSPR"
     gene            complement(4246..4902)
                     /gene="rsmG"
                     /locus_tag="PKK81_08670"
     CDS             complement(4246..4902)
                     /gene="rsmG"
                     /locus_tag="PKK81_08670"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNV32990.1"
                     /translation="MEHDELQFEKSLQSLAAEFSISIHDSSVSYFWRYLSELKKWNRA
                     INLTAIDDDREILVKHFIDSIYGLKVIDINSEIELLDVGAGAGFPALPLKFVRPQLSV
                     ELLEPSEKKGAFLRYVVGSLGLQDVTVATAKLEDYAGRGNVQGRFDYIVVRAFKVDQF
                     GVALSTLLKPDGKLVLYRAEKVPRSFELVGLALIKEVEYELPARYGHRVLSVYSKALR
                     "
     gene            complement(5006..6880)
                     /gene="mnmG"
                     /locus_tag="PKK81_08675"
     CDS             complement(5006..6880)
                     /gene="mnmG"
                     /locus_tag="PKK81_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNV32991.1"
                     /translation="MSEQCDVIVVGGGHAGCEAALAAARMGVRTILLTMDPDRIAQMS
                     CNPAIGGIAKGHLVKEIDALGGEMGRNTDQAGIQFRMINTSKGPAVRALRAQCDKKVY
                     REVMQRTLRAQPALEIRGGTVDRILTRGGAVTGIVTDAGTSIHARAVVLTSGTFLKGL
                     IHIGLNHFPAGRAGEASAEHLSDCMRDFGFEVGRLKTGTPPRLDRDSIDFSVMELQPG
                     DDPPPPFSYRTLRVSLPQVPCHLTYTNSQTHDIIAKNIDRSPLFSGVIGSVGPRYCPS
                     IEDKVVRFADKDRHQIFIEPEGLDTNEFYPNGISTSLPVDVQAAILKTIPGLEQARML
                     KPGYAVEYDYFPPRQLHNSLETKLVAGLYHAGQINGTSGYEEAGAQGIMAGINAALRW
                     RGEEPLVLDRSQAYIGVLIDDLITKDAREPYRMFTSRAEYRLLLRHDNADLRLMDLGH
                     RIGLVSDEVHGKLEAKRSAIEREVQRLKETRPKITSDVKRLMEEAKIGTVAPTQSLAE
                     ILRRQEMTYEKIVAVFGGANIDDLDIAEAIQLEVKYEGYIKRQLQQIQRSEKLEHRAI
                     PDQFDYDGIAGFSGEVREKLKRVKPASIGQASRISGVTPAAISLLLVAIERHRRQPSA
                     "
     gene            complement(6894..8324)
                     /gene="mnmE"
                     /locus_tag="PKK81_08680"
     CDS             complement(6894..8324)
                     /gene="mnmE"
                     /locus_tag="PKK81_08680"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNV32992.1"
                     /translation="MHGALDDTICAIATPPGEGGIGVVRISGLQAVDVASQVVRLRSG
                     KSLHDLQTHVMALADVGSPGALQTVPRGTESRPHAVLDEALVVVMKGPHSYTGEDVVE
                     VQCHGGPVVLDQLCLGLISAGTRLAEPGEFTKRAFLNGRLDLAQAEAVLDTIRAKTAR
                     SLAIAQSQRRGELSREVEETRSALVVALAHIEAALDFAEEDIAFVRQDELLRLLDETL
                     SKLRGLVQSGQEGRIWREGAAVAILGRPNVGKSSLMNALLRSDRAIVTPIPGTTRDML
                     EEVVSIHGIPVRLFDTAGIRATDDPVEAEGIRRSHLAWEDADLALILLDGSQPLLESD
                     RLLLRRREAAQALLVVNKCDLPSRISKEDLIRVCPVNAGVLDISAKMQIGLDGLRDAI
                     SSRLMPRGLESREGVLVTNLRHAAAFERALQGVEQARQSVEAGMAGELVAMDLRIAAD
                     ALGEITGAITTDEILERIFAEFCIGK"
     gene            complement(8346..>9334)
                     /gene="yidC"
                     /locus_tag="PKK81_08685"
     CDS             complement(8346..>9334)
                     /gene="yidC"
                     /locus_tag="PKK81_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNV32993.1"
                     /translation="EAERKGDVKWSAIQDKYFLSVLMPQKAAAALAKKEGDKLVSAGV
                     RFATPESEATMAMQLYAGPKEYDTLKRLNVGLEDTIDFGWFVFGSWGLVKAVAKPIFY
                     VLRFLYEFTHNYGITIILLTMLIKLMFVPLQYKSYKSMKQMQVIQPKVLALQTKFKDD
                     RERLNKELIKLYKDHRVNPVGGCLPMVLQMPVFVALFNILYMTIDLRQAPFMLWIKDL
                     SVQDPYYVLPIVMGATMVIQQKITPTTMDPTQAKIMLFLPVFMTFLFVNFPAGLVLYW
                     LTNNTLTITQQVVTERLFGKKWQVAAADADAVTGDEPKEQKSSGKRRNSPSE"
BASE COUNT         2050 a         2971 c         2332 g         1981 t
ORIGIN      
        1 gttttgatca aacgggaagc ttgcgtgcgg tgggcacgac aaaaagcgcg ccaagttagc
       61 attcaaaagc gggtactgtc aagaagtgat gcgtggggag accgcagatg agacatcgca
      121 acaagcatga gacccgcgcc agtgccgctt gcgcacggcc tccactgagc ccccgcttcg
      181 caaaattgat ttactggaga aaaaattgga gagtggcact ggctatgacc gctgaatgat
      241 gccgccaccg agtacgcggt cgccatcata aaacacggcg gactgacctg ggctcaaggc
      301 gcgctgcggc tgatgaaatt gaacctgaag cgtgccgctt tcagacggta gaagggtcgc
      361 cgggcaggga ggcgtggcgt aacgaatctt cacgtccgcc tcaaccggct gccgaccgag
      421 ggcgtgatca aacagactga gatcggcgac ctgacactct gactgcacaa gctgatcttc
      481 cgcacataac accacctgac cggattcagg caccactttc tgcacgtaca accgctgccc
      541 cacggcaata cccaggccgc ggcgttgccc cggcgtatag aacgcgatgc cctcatgctg
      601 cccgaggacc tcgccatcca cccccacaaa gggtccgggc ttcttagcct ccggcatttc
      661 ctgctcgatg aacgttcgat agtcgccgtg gctcacgaaa caaatctctt ggctttcctt
      721 gagttcctcc acgggcaatc cgagcgattc ggcctcttgc cacacatcgc acttctgcat
      781 atggcccacc ggaaacagca ggtgcggcaa ccaggcagga ttgatccgat agagaaagta
      841 actttggtct ttgcgcgcat caagcgcccg atgcagcgac cactgcctat cgatctgctg
      901 cacgcgggca tagtgccccg tcgccacata gtccatgcct cgctcatgtg ccaaggcata
      961 cagggagcgc aatttcaccc gctcattgca ccgcacgcag ggattgggcg tggtgccgga
     1021 ggcatacccg gcgacgaaat catcgatcac gccctgctga aagacttcgc gacgatcgac
     1081 aacctcatat ggaatacgta gacgttgcgc gacatacttc gcgatcccga tcttgcagca
     1141 tccccgctct tcccagcgct tggagacggc gacctggtca tcctcatgct cccatacctg
     1201 gagcgtgacg ccgtggacat cgtacccctg ccgcacgagg agcgaggcag cgacggagct
     1261 atccactccg ccgctcatac ctagcagtac ggtctgacgt gtcataaatg gtaccggagg
     1321 aacaatgcgc cgagcaccta cggctcttgc gttttgactt tgccgcgatg ggctacgagg
     1381 tcgcgcacgc cgggatcctg cggagcaccg aattcagagg actcgtcctc cgtccacttt
     1441 tcggcgccca tattgctcat gccgtgaaaa tgcgcgcctt cttcgatcat gatcaccggg
     1501 ctctggatat ccccgatgag aatccccggc ttttgaatct cgaccttctg cagagccgtc
     1561 accgacccct tcacgcgtcc actgatcgag acggatccgg cagcaatcac gcccttgatg
     1621 acggcgcttt ctccgacgat caccgccccg cccgtatgca cttccccctc gacacggcca
     1681 tcgattcgga cggtgccatc aaacgtaaca atgcccttaa agctggtccc cttgccgaga
     1741 aaggtaaatt tatcgtcttc cgcgaccggc tgctttttgg tctcacccca catcggcgtc
     1801 ctccttcaca caccccacac atcctagaga ccggggcgca ggctgaatgg aaccttgcta
     1861 tgcctacctc ttgcttacaa ggcaagccag accctgtcca acaaattcgt cgttgagccg
     1921 agttatccgt tgaccaactt gacgccctgt ccgcgcgtgg atccctgccc ctgcggctcg
     1981 cgcgttcccg actggctcat ctccaacgtg ccgttgaaga ccaccccttc ttccatggcc
     2041 aacagcggcg ccttcacgcc ggcattcacc acggcaggcg cgcgcaactt caccttctcc
     2101 cgggcgctga tgtcgccggt gatcttccct ttgcacacca cggtaccggc cgtgatcttc
     2161 gccgtcagaa ccgcatcctc accgaccagc aacactcctt cggtatgaat ctctccatcc
     2221 agattgccat cgatgcggac ggtgccgtta taggaaatga cacccttgaa gctgacacct
     2281 tttccgacaa aggcattgat ctcttcgttt ccctcgcgcg gtgccgtaga ttccaaattt
     2341 tccatttcag ttccctcgct ttgtcctgcc tgacgcttcc cgtcctgctt gtccttatta
     2401 atccacgcca tttgttgcac ctcccacctg ttacaacgtc gtccatgatc ggaccggcag
     2461 gctttcaatt acctgccgga cggtcaccac taagagtgtc cccatttaat atccggtgaa
     2521 aatcgccgat attttactct cgcagcgagc agaaaatctc gcctaaaaca catctgccat
     2581 caattgagca gccgctcgac gacgccgtcc aattcttccg gcgaaaaata atggatgacg
     2641 atcttgccgc cgcgccgccc cttctgaaca tccactcggg tccccagacg tttctgcaat
     2701 cgctcttcga gatccgatcg taatggcgca cgaaccggac gtttccccgg ctttctcgct
     2761 tccgcatgtt cctgtaccaa tcgctccgct tcgcggacgg acagttgccc gctgacgatc
     2821 tgtgtcgcca aactgacctg cgcggccggt gtcatcagcc cgagaatgac cttggcatgg
     2881 cctgtggaga gctgatcgga ttcgatcatg tgttgcacct cggagggcag cgacatcaat
     2941 cggagcaggt tcgccacgga cgaccggtcg cacgccactt tctgagcgat cgcctcctgg
     3001 gtaagtccga attcattcag catgcgttgg tacgcccgcg cggtctccat gggattcagg
     3061 tcgtcccgct ggaggttctc caccaacgcc aacaccatcg actcctgatc ggacacgttt
     3121 cgcacaagag ctggaatctt ctgcattccc gctaacttag cagctctcaa gcgtctctca
     3181 ccggcaatca actcatagat gccatcacct tttcgtcgta ccagaatcgg ttgcagcaag
     3241 ccattttgct tcaacgaggc ggtcaactcg gccagttcga cctcggagaa ttgctgccgt
     3301 ggttgaaagc ggtttgggac gatcgcttcg agccgcaact cctggacatc accacgctcc
     3361 ggctctgccg ttgttcggcc ggtcggcaat agtgcgtcga gtcctctacc gagggctttt
     3421 ttctccatgg accacaaact ccttggctag ggaaagatag gcttgggcac cggcggatgc
     3481 catgttgtag agcagggccg ggcgaccata gctcggtgcc tcggccaacg tcacgttgcg
     3541 gggaatcatc gtttggtaca cgctggcccc gaaatgcccg cgaatctgtt cgaccacctg
     3601 gcgggccagc gaattgcgcg catcgtacat cgtgagcaca atgccctcga tttccaaccc
     3661 agggttcagc gactgccgga gacgctgaat actctccatc agccgaccga ggccttccat
     3721 cgcatagtat tcgcattgca ccgggatcaa cactgagtgc gccgcgacca tcgcgttgat
     3781 ggtcaggagg ccgagggccg gtggacaatc cagcagaatg gtgccgtagc ggtcagacac
     3841 ctcagccagg gcctctttga gacgctgctc acggtcctcc atatttacca gctcaacctc
     3901 tgcaccggcg aggtgggaat tcgccgggac aatcgacaac ccgttcactc cggtctgcat
     3961 agccagagac tcaatactct ccttactaat caaggcattg tagatcgtct tcgtgagcga
     4021 catggcgtcc acaccaagcc cgctcgttgc gtttccctga gggtcaatat cgacgaggag
     4081 aaccgatcct ccctcgattg ccagcgctgc ggcaaggttc acagaggtgg ttgttttccc
     4141 cactccaccc ttttgattcg caacagcaat gattcgagcc atgtagttcc cctatccact
     4201 caaacaaaga tatgtgcgcc ccaatgttcc acgtggaaca ccatctcatc tcaacgcctt
     4261 tgaatacacg gacaacactc gatgcccgta cctagcagga agctcatact cgacttcctt
     4321 gatcaaagca aggccgacaa gctcaaaact cctaggaacc ttctccgcac ggtataaaac
     4381 aagctttccg tccggcttca gaagcgtcga cagcgccacg ccgaactgat ccaccttaaa
     4441 ggccctcacg acgatgtaat caaaccgccc ctgaacattt cctcgaccgg cataatcttc
     4501 caacttggcc gtcgcaaccg tcacgtcctg cagaccaagc gatcccacga catagcgcaa
     4561 aaacgccccc ttcttttcgc tcggctcaag caattcaacg gacagctgag gccgtacaaa
     4621 tttgaggggc agagccggaa atccagcacc ggcaccaaca tcgagcaact caatttcact
     4681 attaatatca atgactttaa gcccatagat tgagtcaata aaatgcttca ccaggatctc
     4741 tcgatcatcg tcgatggccg tgagattgat ggccctattc cattttttca gctcgctgag
     4801 gtaacgccaa aaataagaaa cagaggaatc atgaatggaa atggagaact cagcggcaag
     4861 agactgaaga gatttttcga actgtagctc gtcgtgttcc acgtggaaca cttacgcgaa
     4921 aagaaccaac agggtcaagc aagttcttcc ttatcatgcg cacagtgtta gcgagaatcc
     4981 attcccggca atccagcaca ccatttcatg ccgatggttg ccgacgatgt ctctcgattg
     5041 caaccaacag aagtgaaata gcggcaggcg tcacaccgga aatacgcgaa gcctgcccta
     5101 tcgatgccgg cttcacacgc ttgagcttct ccctgacttc acctgagaaa ccggctattc
     5161 cgtcataatc aaactgatct ggaatcgctc tgtgctctag cttctccgat cgctgaatct
     5221 gctgcagttg ccgcttaata tatccttcgt atttcacttc gagttgaatc gcttccgcga
     5281 tatcaagatc gtcaatatta gcgccaccga acactgcgac gatcttttca tacgtcattt
     5341 cctgacgcct gagaatctca gccaacgatt gcgttggtgc cacagtgcct atcttcgcct
     5401 cttccatgag ccgcttcaca tcagacgtta ttttcggcct ggtttccttg aggcgctgaa
     5461 cttcccgttc aatagcagac cgcttcgcct ccagcttccc gtgcacctca tcagatacca
     5521 atccgatgcg gtgcccgaga tccatcaggc gaagatcggc gttgtcatgg cggagcaaca
     5581 gacggtattc cgcgcgcgag gtgaacatcc gatagggttc tctggcatct ttcgtaatta
     5641 aatcatcaat taacacgcca atataggcct gcgaccgatc cagcacgagc ggctcttcac
     5701 cacgccacct caaggccgcg tttattccag ccataattcc ctgggcaccg gcctcttcat
     5761 acccggacgt tccattgatt tggccggcgt ggtagagccc ggcaactaat ttcgtctcta
     5821 gcgaattgtg aagttggcgg ggaggaaaat aatcgtactc gaccgcatac ccaggcttca
     5881 gcattctcgc ctgctcaagt ccgggaatag ttttcaggat ggccgcttgc acatcgaccg
     5941 ggagactggt tgaaattcca ttcggataaa actcgttcgt atccaaccct tccggctcga
     6001 tgaagatctg atgccggtct ttgtcggcaa acctcacgac cttatcctcg atcgatgggc
     6061 aatatcgggg accgacagag ccaatcaccc cgctgaacaa cggcgatcta tcaatatttt
     6121 tcgcaataat atcatgcgtt tgtgagttcg tgtacgtcaa atggcagggc acctgtggca
     6181 aggacacacg cagcgttcgg taagaaaacg gcggtggagg atcatctcct ggctgcagct
     6241 ccatcacaga aaaatcgata gaatcccgat cgagccgggg tggagttccc gtcttcaacc
     6301 gaccaacctc aaatccaaaa tcacgcatgc aatcggagag gtgctccgcg gacgcctccc
     6361 cagcacgacc agcgggaaaa tgattgagac cgatatggat caaccccttc aaaaatgtgc
     6421 ccgacgtaag cactactgcc cgcgcatgaa tagacgtacc ggcatccgtg acgataccgg
     6481 ttacagcccc accccgggtg agaatgcgat caactgtccc gcccctgatc tcaagtgccg
     6541 gctgcgcacg gagcgtgcgc tgcatgactt cgcgatagac cttcttatcg cattgcgcgc
     6601 gcaaggcacg gacggccgga cccttgctgg tgttgatcat ccgaaactgg atgccggcct
     6661 ggtcggtatt tcgccccatt tctccgccta acgcatcgat ctccttgacg agatgtccct
     6721 tggcaatgcc gccgatcgca gggttgcagg acatctgagc gatccggtcc ggatccatcg
     6781 tgagcaggat cgtgcgcacc cccatgcggg ctgcggccag agccgcttca cacccggcat
     6841 ggcctccccc gacgacgatg acgtcacact gttcactcac gacaagagtc ctttcatttc
     6901 ccgatgcaaa actcagcgaa aatgcgctcc agaatttcgt cggtcgtgat agccccggta
     6961 atttcaccca gggcgtcggc agcgatacga agatccatcg caactaattc tcccgccatc
     7021 cctgcctcga ccgactgtct ggcctgctcc accccctgca acgcgcgctc gaaagcagcg
     7081 gcatgccgaa gattggtcac aaggacccct tcccgggact ctaaacccct cggcatcaat
     7141 cggctgctga tcgcatctcg caatccatcc agtccaatct gcattttcgc agagatatcg
     7201 agcacaccgg cgttcacggg acagacgcgg ataaggtctt ctttcgagat ccgagatggc
     7261 agatcgcact tattcacgac caacaacgcc tgcgcggcct cccgacgacg aagcaacagc
     7321 cgatcgctct ccagcaacgg ttgcgagccg tccagaagaa tcagcgccaa atccgcgtct
     7381 tcccatgcga ggtggctccg acgaatgcct tcggcttcga cggggtcatc cgttgcgcgg
     7441 atgccggccg tgtcgaagag tcggaccgga atgccatgga tactgacgac ctcttccaac
     7501 atatcccgcg tcgtaccggg aatcggcgtg acgatcgcgc ggtcgctccg taacagggca
     7561 ttcatcaaac tcgacttgcc gacgtttgga cgtccgagaa tcgccactgc ggcgccttct
     7621 ctccagatcc gtccttcctg accggactga accagacccc gcagcttgct caaggtctca
     7681 tcaaggagcc gcagaagttc gtcctgccga acgaatgcaa tatcctcttc cgcaaaatcc
     7741 agcgcggcct cgatatgcgc caaggccacg actaaagccg accgcgtctc ctccacttca
     7801 cgggacaact cgccgcgccg ttgcgattgt gcaatcgcca ggctacgcgc agtcttcgcg
     7861 cgaatcgtat cgagcacggc ctcggcctgc gccagatcca gtcggccatt aaggaacgcg
     7921 cgcttggtaa actcccccgg ttcagccagt ctggtccctg cggatatcaa gcccaggcac
     7981 agctgatcca gcacgaccgg ccccccatgg cactgcacct cgacgacatc ttcgccggta
     8041 tacgaatgcg gccccttcat cacgactacg agcgcttcat cgagcaccgc atgcggacgt
     8101 gattccgtcc cgcgaggaac ggtttggagc gcgccgggcg agcctacgtc cgccagcgcc
     8161 atgacatgag tttgaaggtc gtgaagggat ttgcctgacc ggaggcgcac gacttgagaa
     8221 gcgacatcaa cggcttgaag cccgctgatg cggacgaccc cgattccccc ttcgccggga
     8281 ggggtggcga tagcacagat ggtatcgtcc agcgcgccat gcatgacgca cccaatcgtg
     8341 tggaattact cggatggact gttgcgccgc ttgccgctgc tcttctgctc tttcggttcg
     8401 tcgccggtca cagcatcggc gtccgcagca gccacttgcc acttcttccc gaacagccgc
     8461 tcggtcacca cctgctgggt gatcgtaagc gtattgttcg tcagccaata gagcaccaat
     8521 ccggcgggga agttcacgaa caggaacgtc atgaacaccg gcagaaacag catgatcttc
     8581 gcctgcgtcg gatccatcgt ggtcggagtg atcttttgct gaatgaccat ggtggcgccc
     8641 atgacgatcg gcagcacgta gtaaggatcc tgcacagaca ggtctttgat ccagagcatg
     8701 aacggggcct gccgaagatc gatcgtcata tacagaatat tgaacagcgc cacgaagacc
     8761 ggcatctgca gcaccatcgg cagacagccg ccgaccggat tcacccgatg atctttatag
     8821 agcttgatca gttccttgtt cagccgctca cgatcgtcct tgaacttcgt ctgcagggcc
     8881 agcactttcg gctgaatgac ctgcatctgc ttcatggact tgtagctctt gtactgcagc
     8941 ggcacgaaca tcagcttgat cagcatggtc aagagaatga tcgtgatccc gtagttgtgc
     9001 gtaaattcgt agaggaatcg cagcacatag aaaatcggct tggccaccgc cttgaccaac
     9061 ccccaactcc caaatacaaa ccagccgaaa tcgatcgtat cttccagccc gacgtttaag
     9121 cgtttcagcg tgtcgtactc tttgggcccg gcgtagagct gcatcgccat cgttgcctcg
     9181 ctctcgggcg tggcgaagcg gacaccggcc gacaccagct tgtccccttc cttcttcgcg
     9241 agggcagccg cagccttctg cggcatcaac acacttaaga aatacttatc ctgaatggcc
     9301 gaccacttca cgtcgccttt gcgttcagcc tcgc
//
LOCUS       DAOQRM010000096         9327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6798_length_9327_cov_11.756471, whole genome shotgun sequence.
ACCESSION   DAOQRM010000096 DAOQRM010000000
VERSION     DAOQRM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9327
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6798_length_9327_cov_11.756471"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(719..1273)
                     /locus_tag="PKK81_08690"
     CDS             complement(719..1273)
                     /locus_tag="PKK81_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32994.1"
                     /translation="MKHLSLYALMVVVAAGISLPCEALEFTAERLSRTGQEVHHARIF
                     YRDHMWRLEYNEPGAVSATIVRADRNQVWHLIPSIHHFRTESYGSDYALHLTIRLDNE
                     TSREFIGTQTLDGHPTTLYEVVATGPGEQKETYYQWVATDMNFPLKLVKKDGDWMVEY
                     RDVKLGRLADSFFQLPHRYVPMEP"
     gene            1940..3325
                     /locus_tag="PKK81_08695"
     CDS             1940..3325
                     /locus_tag="PKK81_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV32995.1"
                     /translation="MSTSHARILLVDSNADAAQELRDILSHEGYEVSSARDPKSARRY
                     FEQGSADLILTDLQLPQLDGLTFLTEMRTRFPNMPVVLMTANGSLRTAVEGLKAGAFD
                     YLTKPFIVDEVRCVARRALEHTSPPSESPTASDLQPEDASPFGSLVGSSPRMVHVYKA
                     IARVAQTDSTVLLQGESGTGKELIARAIHVSGPRSSGPFVTVDGGALADSLLESELFG
                     HERGSFTGAVSSRKGLLEKAHSGTCFLDEVADLSPALQAKLLRVIQEKEIRRVGSSAS
                     TSLDVRIIAASKLPLEPLVRAGTFREDLYYRMNVVTIDIPPLRERPEDIPALAQRFLN
                     LYGAIKIPSVKEISSEAMELLSRYWWPGNVRELEHAIERAVALSPHAVIYPEDLPPVV
                     KNAAPESLARAQGWVTLENLERDHILRVLDSHRHDVGRSAVILGIHRKTLLRKLRQYG
                     MATAAPAHGSL"
     gene            complement(3346..4197)
                     /locus_tag="PKK81_08700"
     CDS             complement(3346..4197)
                     /locus_tag="PKK81_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32996.1"
                     /translation="MKCITRSEYSYIGIRMMICFGLIGMWGALLPMAESAEAGQRISP
                     GEGAQLRDDAEKLLKGNRSVLGKVLAITSDQIKVDIGEVQPRFLPLKQAQQKGFPGVA
                     EGDDLIVVLNAQNLLVDYHPLDGQASAHTIIRGEIAQNLPVGQEQVVIKSAGKEMSFT
                     IRSQARSKVAAIPVGVAAVFLIDETNQIADVGLSGQQTSKQADRLTDSMPPIKGAHKQ
                     IDGTVTSPLRANHITVRTANGSEKPFEVRELLHKKLAALRKGDAVILLVDTDNKVIDV
                     AVPPPGR"
     gene            4568..5653
                     /locus_tag="PKK81_08705"
     CDS             4568..5653
                     /locus_tag="PKK81_08705"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV32997.1"
                     /translation="MQATTTKELVLVTGSSGLIGARTADRLSAEFRVAGLDRPGEPHP
                     PSSVENIPCDLTSDDSVVQALTMVKGRFGSDIAAVVHLAAYFDFSGEPSSKYSEVTVE
                     GTRRLLRALHTLGFRVARFIFSSTMLVHKPCEPGEAITEEWPLDPRWPYPQSKVETER
                     MLEKERGNYPTAVLRIAGVYDDDCDSIPLAHQIERINERHITSKMFPGHISHGQSFLH
                     LEDLVDAIVRVVERRRSLPDQAVMLLGEPEPLTYDELQHRFGRLLHGEEWDTVRIPKM
                     VAKTGAWLQGVTPLLEDPFIKPWMIDFADDHYALDVSRAKEWLDWEPRHSLRDTLPKM
                     IDALKRNPVAWYRAHQLSPPAELEETQ"
     gene            5650..6213
                     /locus_tag="PKK81_08710"
     CDS             5650..6213
                     /locus_tag="PKK81_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNV32998.1"
                     /translation="MTGTDALRPPGWTYNPSSWSERLWLIGVAILGFLISGYLGLYQW
                     GILDSVWEPWFGRGSETVLHSPLSRVLPVPDAIVGAMAYGIDAVAGAIGGTRRWKTMP
                     WMVVLFGLAVGPLGLVSVLLVTAQPLLLHAWCTLCLLSAVISVVMIGPAMDEVLASLQ
                     VLKRAKEQRKAVWTTFWKGEGKAVWSG"
     gene            6239..>6572
                     /locus_tag="PKK81_08715"
     CDS             6239..>6572
                     /locus_tag="PKK81_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV32999.1"
                     /translation="MWPPAVTGMLGVWLAAAPDVMGYGGHARLSNQFVGVWMAAFGAI
                     AMSECLRAVQWANLVLGVWLVAAPFLLEYPDERACGSLVVGIAAISLALVRAPLRDRF
                     GGGWTSLWR"
     assembly_gap    6573..6672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7025..7570
                     /locus_tag="PKK81_08720"
     CDS             7025..7570
                     /locus_tag="PKK81_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNV33000.1"
                     /translation="MRNHMSAGMREKAVFVVAREMVGRQGMYSRPASATAGEAERRAY
                     RLLTLAGFTIVIFTADALAVCAECHEGNSTCDAVRYSTRPTPLSKQIMPLLDATAALH
                     IDLACSWLVGDRLDNIEVGRLLGCNTILCLNGSETDWDMTAMRWPHFIVRDIWETACL
                     IVNAGGTFVASVSDEESDQDD"
     gene            8020..>9327
                     /locus_tag="PKK81_08725"
     CDS             8020..>9327
                     /locus_tag="PKK81_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33001.1"
                     /translation="MRTSRRRRIATAGLLGASVFSIAIVSGLVFVGAQGHGEEKRNQP
                     QSAIEKAKEKGFDGVDFSYDLFGAPSGQSTAHIAEEVMRKDMADKPRVMANHAALLQA
                     RYTLECKTQTGAIMTKGKPQPIGPTVQLKEGMTWDKLSHISPEDIRRQKLFPSGFMRL
                     PHVKHEVGGQVFPQKQVEQFPRLERFDVDFDLPDCFLPEFPPPIFLTTHPELGDVSQG
                     EVLSADNFDRLFRGLITPVQLDGLRMLVTQFPQEEFNLTPDRKSPKPSLGVACLDCHV
                     NFHTTGQFHLNPDTRPQRDRLRLDTVSLRGMFNQQVHGSKRSLRSIEDFTEFEQRTAY
                     FNGDPIRAMKKGMNVIDRLQVAHMAQIQNMIDFPPAPKLDPMTGRLDRARASQREIAG
                     EDLFFGKARCVTCHQPPAYTDHMLHDLFLERFGAEADGPIKSFA"
BASE COUNT         1913 a         2546 c         2752 g         2016 t
ORIGIN      
        1 tcgaccccct ctcggggcaa agccggatcg cgatcagatt ctgaatgtgt ccgggaagtt
       61 ttacggcggt tccttccact ccctagcgct catcccatat caggtttgtc gacgaaccca
      121 tgtaatcggc gatcatcgtg ggcgttgcga ttgcacgtcg gtgtcggccc ctgtggaagt
      181 cagtagattc tgaatcttct gaggctcccc gtcggttgta acggtgatga gcacattgcc
      241 ccgatgcacc gcttgctcct aatggtggag cgcggtttca ggaatattga tactgatcag
      301 cgctccgacg aggccaccgg tgacagcgtc gagtccgctc gctgccacaa tggaggcacc
      361 ggttgctccc aatataagaa atgagcgatc cacctgccag caggggagcc agaccgggga
      421 tagttgcgaa gcctgccgct gtcagcaacc cggcaagcct tccaatgata ccgcctccta
      481 caaccccctg ggtggcctct tccgctgtgg gggaagggcc gatcctcctc ggtcgttgag
      541 aggggttgtt ccgcttgccg catggccagt ccgatctgct cgtctgaaac accctgcaaa
      601 cggagagatt caagagccag tctggcggca gccacgctcg gcaggaggcc gacgatgaca
      661 tgaccgcctg ctccgggggc ctgatgcagg gacgttgaat tggtcataaa ggatcccctt
      721 atggctccat cggcacatac cggtgcggaa gctggaaaaa cgaatcggcg agccgtccga
      781 gcttgacgtc acgatattcc accatccagt caccgtcctt tttgacgagc ttgagtggaa
      841 aattcatatc ggtagcgacc cattgatagt aggtttcctt ttgttctccg ggaccggtcg
      901 caaccacttc gtacagcgtg gtcggatgcc catctaaggt ctgcgtgccg atgaattctc
      961 gtgatgtctc gttatccagt cggatcgtga ggtgcagcgc atagtcgctg ccgtatgact
     1021 ccgtcctgaa gtgatggatc gaggggatca ggtgccagac ctggttacgg tcggccctga
     1081 cgattgtcgc gctgacggcc ccaggctcgt tgtattccaa ccgccacatg tggtcgcggt
     1141 agaagatccg ggcatggtga acttcctgac cggtacggga tagtcgctct gcggtaaatt
     1201 ccaacgcctc gcatggaagc gagattcctg ccgcgaccac taccatcagc gcatacaggc
     1261 tcaggtgttt catacctgcc ccattctgtg gagggcgaga ccaaacctgc cgtgaggtga
     1321 ctctcgccca gagcgttgca ccggagtgct ctgagtgttt cgacctgact caatcggaca
     1381 gatgagcaaa cggcatgccg gagggcgcac tctctaaaat gcgcgcgatg gagtgcgagt
     1441 gggggcaacg gcgtggtcgt gaggggagcg aagtatcccg cgcgggacaa cttgtcctcc
     1501 gtatgagacc actattttct tcacaactga acatcaggag caaccggggt taatggggag
     1561 gggcaggggg gatcggcttt ggagcaggag ccgggatcgg tgacggagga atcggttttg
     1621 gaatcggccc tggaatgggc gcaggtggaa taggctctgg tttttgaaga atctgagcgc
     1681 tggtgacagg aggaatgagc gcgctcagga gcgccaggaa gcatgtgatg atgcggcaat
     1741 gatgaccaac gccaggcaga ggaggaggac tactcacgtc atgtctccaa ccattccatg
     1801 ctgtaccacg ctgaggccga ctcatggttc tcgattgcct cgtgggccag ggtttcttcc
     1861 aggttacgat gtcgcccctg gggcgtactc tacaatttct atactgggcc tgattgattg
     1921 ccttcaggaa cgtttacaga tgtctacctc acatgcccga attcttctcg tcgacagcaa
     1981 cgctgacgcg gctcaggaat tgcgcgacat tttgtcccat gagggttatg aggtgtcatc
     2041 cgcgcgtgac ccgaaaagcg cccggcgata tttcgagcag ggatcagcgg atctgatttt
     2101 aacggatttg caactgcctc agctcgatgg cctgacgttt ctcactgaaa tgcgcacccg
     2161 cttcccgaat atgcccgtgg tgttgatgac ggccaacggt tctctcagga ctgccgtaga
     2221 ggggctgaag gccggtgcct tcgattattt gacgaaacca tttattgtcg atgaagtgcg
     2281 atgcgtcgcc agacgggcac tagaacatac ctctccgcct tcagagagtc ccaccgcctc
     2341 agacctgcag ccggaagacg cttcgccgtt cggtagtctc gtcggcagta gtccacgcat
     2401 ggtgcatgtg tacaaagcga tcgcccgggt ggcacaaacc gacagcaccg ttttattaca
     2461 aggcgagagc ggtaccggaa aggaactcat cgctcgcgcc attcacgtca gcggccctcg
     2521 aagcagcggc cccttcgtca ccgtcgatgg gggggcactt gcagacagtc tgctcgaatc
     2581 cgaattgttc ggacatgaac gtggatcttt taccggggcg gtgtcatcgc gcaaaggtct
     2641 ccttgaaaag gcacattccg ggacctgttt tctggacgag gtcgctgact tgtctccggc
     2701 tctgcaagcc aagctactac gagtcatcca agagaaggag atccggcgtg tcgggagcag
     2761 cgcttcgacg tcgctggacg tgcggattat tgcggcttcc aagctgcccc tcgaacctct
     2821 cgtccgcgca gggacattcc gcgaagatct ctattatcga atgaatgtcg tcacgatcga
     2881 cataccgccc ttgagagagc gcccggagga cattccagcc ctggcgcaac ggttcctcaa
     2941 cctgtacggg gcgataaaaa ttccgtcggt gaaggagatc tcctccgaag ccatggaatt
     3001 gttgagtcga tactggtggc cgggcaacgt tcgggaactc gaacatgcga ttgagagagc
     3061 cgtcgccttg agtccgcatg ccgtgatcta tccggaggat cttcctccgg tcgtcaagaa
     3121 cgctgcgccg gagtccctcg cgcgcgctca gggatgggtg acgctggaga atcttgaacg
     3181 agaccacatc ttgcgggtgc tcgattcaca ccggcatgat gtgggtcgat cggccgtcat
     3241 tctcggcatc catcggaaga cgctgcttcg gaagctccgt caatacggca tggcgactgc
     3301 cgctcccgcg catggcagtc tatgaatgcg cgggagcatt tgtgctcacc gtcccggcgg
     3361 tggcaccgca acatcgatca ctttattatc tgtatctacg agcagaatga cggcatcccc
     3421 tttacgaaga gcggcgagtt ttttgtgaag gagctcccgt acttcaaaag gtttctcaga
     3481 cccattggcc gttcgaacgg taatgtgatt ggcccggagc gggctggtaa cggttccgtc
     3541 tatctgctta tgggcgccct tgatgggcgg catggagtct gtgagtcgat cggcctgttt
     3601 gctggtctgt tgaccggaca gtcctacatc cgcaatctga ttcgtctcat cgatcaagaa
     3661 cacagcggcc acgccgacgg gtatcgcggc aactttgctg cgtgcctgtg aacggatagt
     3721 gaaggacatt tctttccctg cggacttaat cacgacctgt tcctggccca ccggaaggtt
     3781 ttgcgcgatc tctcctcgaa taatcgtatg ggcgctggct tgcccgtcca gcgggtgata
     3841 gtccaccaag agattttgcg cattcagcac gacgatcaaa tcatctcctt cggcgacacc
     3901 agggaaccct ttctgctggg cctgtttgag ggggaggaag cggggctgca cttccccgat
     3961 gtcgactttg atctgatccg acgtgattgc caacaccttt cctaagacag agcgattccc
     4021 tttcaggagt ttttccgcat cgtctcgcaa ttgagctcct tcgcccggac tgatacgctg
     4081 tccggcttcc gctgattccg ccatcggtag caaggcgccc cacattccga tgaggccgaa
     4141 gcagatcatc attcgaatgc cgatgtatga atattcgcta cgagtaatac atttcatgat
     4201 gactccctca tgttgagtgt ggtcacgcct actctcttag caagagccgc gccttttcac
     4261 tccaaccata ataggtctgt tctgttccac cagagggcag ctcggacgac catggcccgg
     4321 ttgtcatgat gaggctgatc cccggatctc ggggacaaaa tgtctctcat gggacaatcc
     4381 atccgaactg agagccttcg cgagacatga ttcaggtctc agtcctcgcg aataagaaga
     4441 ttcccccggc tttaaaaggg tggctgtaag gggcgaactc gcccgttcat ggcaatgata
     4501 gagctgtaat gtctggatgg ggctatctga atgcgcttcg gcattgctct tgctgagcac
     4561 aggtcgcatg caagcaacca cgacgaagga gttggtgttg gtgacaggaa gcagcgggct
     4621 catcggcgct cgaaccgcgg accggctgtc tgctgaattt cgagtggcag gactggaccg
     4681 gcccggcgaa ccgcatccgc cctcctctgt ggagaatatc ccctgtgatc tcacctcgga
     4741 cgacagcgtc gtccaggccc tcaccatggt taaagggcgc tttggctcgg atatcgccgc
     4801 ggttgttcat ctggccgcgt atttcgattt ctcgggtgag cccagttcca agtacagtga
     4861 agtcacggtc gaggggactc gacgcctgct tcgcgccctg cataccctcg ggttccgggt
     4921 ggcgcgattc atcttctcca gcacgatgct cgtgcataaa ccctgcgaac caggagaagc
     4981 gatcactgag gaatggccgc tcgatccccg ttggccctat ccgcaatcga aagtggagac
     5041 ggagcggatg cttgagaagg aacgggggaa ctatccgacg gccgtcttgc gaatcgccgg
     5101 ggtgtatgac gatgattgtg actcgattcc actggcacat caaatcgagc gcatcaatga
     5161 acgtcacatt acgagcaaga tgtttcccgg tcatatctcc cacggccaat cctttctgca
     5221 tctcgaagac ctggtggacg ccattgtgcg cgtggtcgaa cgtcgccgga gtctgcctga
     5281 ccaggccgtg atgctgctgg gtgagccgga gccgctgaca tacgacgaat tacagcaccg
     5341 attcggccgg ctactgcatg gcgaggaatg ggacaccgta cgaattccaa agatggtggc
     5401 caagacaggc gcctggcttc agggggtgac gccgctgttg gaggatccgt tcattaaacc
     5461 ctggatgatt gattttgccg acgatcatta cgctttggac gtgagtcgcg ccaaggagtg
     5521 gctcgattgg gagccccggc attcacttcg ggacacgctg ccgaagatga tcgatgcgct
     5581 taagcggaac ccggtggcct ggtatcgggc tcatcagttg agcccccccg ctgagctgga
     5641 ggaaacacag tgacgggaac cgacgctctt cgaccgccag gatggaccta taacccgtcg
     5701 agctggtccg aacgtctgtg gctgatcggc gtggcgattc tcggctttct catttcaggg
     5761 tatctcgggc tctatcaatg gggaatcctc gactctgttt gggaaccgtg gttcgggcgg
     5821 ggcagcgaga ccgtgttgca ttcgccgctc tcacgagtgc ttccggtacc ggatgcaatt
     5881 gtcggcgcta tggcgtatgg gatcgatgcc gtggccggtg ctatcggtgg gaccagacgg
     5941 tggaaaacca tgccctggat ggtggtcctg ttcgggttgg cggtagggcc gttggggttg
     6001 gtcagtgtcc tgctggtgac agcccaaccg cttctcttgc acgcgtggtg cacattgtgt
     6061 ctcttgtcgg ccgtgatttc cgttgtgatg atcggaccgg cgatggacga agtgttggcc
     6121 agtttgcagg tcctgaaacg ggctaaagag cagaggaaag cggtgtggac gaccttttgg
     6181 aaaggcgagg gcaaggcggt atggagcgga taatcatcca aagaaaggcg gcccaatcgt
     6241 gtggcccccg gcagtgaccg gcatgctcgg agtctggttg gcggcggccc cggatgtgat
     6301 ggggtatggc ggccatgccc gcctcagcaa ccagttcgtc ggggtgtgga tggccgcctt
     6361 cggggcgatc gcgatgtcgg agtgcctgcg ggcggtgcaa tgggccaatc ttgtgctggg
     6421 tgtctggctc gtcgccgcac cgtttcttct ggagtatccc gacgaacggg cttgcggcag
     6481 cctcgtcgtc gggattgcgg caatatcgct ggccctggtt cgagcgcctc tgagagatcg
     6541 cttcggcgga ggctggacgt cattgtggag acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nntacttcta gggctcgtgg cggtcgtgct gacgatcggg cggcatccaa
     6721 tcgagggcta cgaccccacg ccggccacgc cataaccgag gagattcagc atgccgaaaa
     6781 aaaagactct cgaagaggcg cgcaaggcga aacgagaggg aaaggctgcg acgacgcaag
     6841 ccggtgcttt cgtgaaagaa gagattgagc acatgaaaac cggcaaacat ccggtgaagt
     6901 ctcgtaagca ggctgtggcc attgggctct cgaaggcgcg gaagtccgga atcaaagttc
     6961 ctcaacgcgc ttcgaattcg aggtccacaa ggtctcgccg gaagtcgagg agttcagcaa
     7021 agacgtgaga aaccacatgt cggcggggat gcgggagaag gcagtgttcg tagtagcgag
     7081 ggaaatggtc gggcgtcagg gaatgtattc tcggcccgct tcagctacgg ccggcgaagc
     7141 ggaacggaga gcctatcgcc tcttgaccct ggcgggattc acgatcgtca tattcacagc
     7201 ggacgccctt gccgtctgcg ccgaatgcca tgagggaaac agcacatgcg atgctgtccg
     7261 ctatagcacc aggccgaccc cgctcagcaa acagatcatg cccctccttg atgcgacggc
     7321 ggcattgcac attgatctgg cctgctcatg gctcgtcggc gaccggctgg acaacattga
     7381 agtcggccgc ttgttggggt gcaacaccat actttgtttg aacgggagtg agactgactg
     7441 ggatatgacc gctatgcgtt ggccccattt cattgtacgt gacatctggg aaacagcctg
     7501 tctgatcgta aacgcaggcg gtacattcgt ggcatcggtg tccgatgagg aatccgacca
     7561 ggacgactga cacgccgtcc tagtcggtcg ggctatcgct gtaccgctat ggcgttgata
     7621 cgatgtcgcg ctcctctgtt gtgacgcgcc tcctcgctct catggtcctt gttccggttg
     7681 ttccggattt gctgatccat gatctccccg caggcgaagc atcgccaggc gggcgagtct
     7741 ttcctcatta atccgccgcc ccagtcctgt aaggcaatcg ggaacatcaa attattgcaa
     7801 cgatgacagt gcatggtgcg tcctcccgtt cggtggcatc aatgacactt atgatcgtaa
     7861 ggaatagcgc tcagagacat cactaggggc aaccctggtt attgacaata ctggtcttca
     7921 tcgccaggga actacctagt agtgcaatag gccatacgcc gctaaattca cactcactaa
     7981 ccatccgcga acgcttctct caaccacatg aggtctcaca tgcgtacgtc acgaagaagg
     8041 cgaattgcca cagcggggct tctgggagcg tctgttttca gcatcgccat cgtgtccggg
     8101 ctggtatttg tcggggctca agggcatggc gaggagaagc ggaaccagcc acaatccgcg
     8161 atcgaaaaag caaaggaaaa ggggttcgac ggtgtggact tttcttacga tctgttcggg
     8221 gcgccatccg gtcaatcgac tgcacacatt gcggaagaag tgatgaggaa agacatggcc
     8281 gacaaaccca gggtgatggc caatcacgcc gcgttgttgc aggcgcgcta tacgctcgaa
     8341 tgtaagacgc aaacaggggc catcatgaca aaggggaagc cgcagccaat cggacctacg
     8401 gtacagctca aggaaggcat gacctgggac aagctctcgc acataagccc ggaggatatt
     8461 cgccggcaga aattattccc atccggattc atgcggttac cgcacgtcaa acatgaggtc
     8521 ggcggccagg tgtttcctca aaagcaggtg gagcaatttc cacggcttga gcgtttcgat
     8581 gtagacttcg acctcccgga ttgttttctt cctgaatttc ccccgcccat tttcctgacc
     8641 acccatccag aattgggcga cgtctcgcag ggcgaggtgt tgagcgcgga taattttgat
     8701 cgcctttttc gcggactcat cacgcccgtt cagttggatg gtttgagaat gttggtgacc
     8761 cagttcccgc aggaagaatt caacctgaca ccggatcgga aatctcccaa gccgagtctg
     8821 ggcgtggcct gcctggattg ccatgtcaat tttcacacca ccggtcaatt tcatctcaat
     8881 ccggacaccc gccctcaacg cgaccggtta cggctggata cggtcagtct cagggggatg
     8941 ttcaatcaac aggttcacgg ttcaaagcga agtttgagat ccatcgagga cttcaccgaa
     9001 ttcgaacaac ggacagccta ttttaacggc gatccgatcc gcgcgatgaa gaaaggaatg
     9061 aacgtcatcg atcgtctgca ggtggcacat atggcgcaaa tccagaacat gatcgatttt
     9121 cctccggcgc cgaaactgga tcccatgacc gggcggttgg atcgtgcacg ggcgagtcag
     9181 cgggagatcg caggcgagga cctgttcttc gggaaggcgc gctgtgtcac ctgccatcag
     9241 cctccggcct ataccgatca catgctccac gatctgtttc tggaacgatt cggtgccgag
     9301 gccgatggtc cgatcaaatc gttcgcg
//
LOCUS       DAOQRM010000097         9196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_6953_length_9196_cov_13.440105, whole genome shotgun sequence.
ACCESSION   DAOQRM010000097 DAOQRM010000000
VERSION     DAOQRM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6953_length_9196_cov_13.440105"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PKK81_08730"
     CDS             complement(<1..1037)
                     /locus_tag="PKK81_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV33002.1"
                     /translation="MAAEIDIQLVKTFPGRAPIKARIRYPVEASTVLILFGPSGSGKT
                     TILRSVAGLEWPEEGTIQFVSRTWLDTASGVRVAPQDRHIGYMAQDYALFPTYTVAGN
                     IGYGLHHLSAQDRNKRVDEMVELFQLRGLETAKPRELSGGQQQRVALARAVAPRPLLL
                     LLDEPLSALDAPTRVHLRDELRSLLKQLALPSIIVTHDWTEALTLGDVMAVISAGNVL
                     QVGPPIEVFSRPHNADVARVVGIETVVKGRVVGSSDGMVQVSVNGTRLTAVEGESAGP
                     DVFVCIRSEDVVLERGQATASSARNHLAGTVISATVLGALAQVTIDCGFPLVAMVTRS
                     TVEEFGLSSGVP"
     gene            complement(1022..1696)
                     /gene="modB"
                     /locus_tag="PKK81_08735"
     CDS             complement(1022..1696)
                     /gene="modB"
                     /locus_tag="PKK81_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNV33003.1"
                     /translation="MNWIAIWVTFKLAASTALVLMIVGLPIAYWVSFSRRRWKFLVES
                     VVALPLVLPPTVLGFYILVAIGPHSPLGRFYNELIGHPLPFTFEGLLLASILYSLPFA
                     VQPFAAAFEQVDRRLIEASWTLGVSKLGTFFKLIVPLSKAGLLTGAVLSFAHTMGEFG
                     VVLMVGGNLEGETRTVSIDIYDEVQALNYAGAAKTALFLLVVSYAILLLVYAVNRNVW
                     AAWPRK"
     gene            complement(1775..1978)
                     /locus_tag="PKK81_08740"
     CDS             complement(1775..1978)
                     /locus_tag="PKK81_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNV33004.1"
                     /translation="MKLSARNQFQGQVVRITEGQAMAEVVVKVGNLEVVSAITEGSVK
                     SMGIKVGDAVTVAVKATDVIVGK"
     gene            complement(2063..2395)
                     /locus_tag="PKK81_08745"
     CDS             complement(2063..2395)
                     /locus_tag="PKK81_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33005.1"
                     /translation="MPALALGAAVLIAGCSEAVLLTHETGQGGVVTYLYKDERGGPMG
                     SPYRREALKVMAEKCSAGYQVVKDGQVQSYSSGSSVEGAEGEEGARRWGIQFECKSGG
                     DDRTTGRK"
     gene            complement(2403..3164)
                     /gene="modA"
                     /locus_tag="PKK81_08750"
     CDS             complement(2403..3164)
                     /gene="modA"
                     /locus_tag="PKK81_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV33006.1"
                     /translation="MARLLLYVMLVLLCRPAIALADDIAVAAASDLQFAIKELIVEYE
                     KQTGHHVKLSLGSSGNFYAQVQQGAPFDLYFSADIGYPKKLEEAGLTVPGSLYRYAVG
                     RVVLWAPKQSPVEVSKGLTVLRDAAVRKIAIANPKHAPYGRAAVAAMEQSQVYAEVKD
                     RLVLGENISQAAQFIESGACDVGIIALSLAMAPVMKSAGSYWLIPAEAHPPLEQGAVI
                     MKQSKQQDAARHFLQFMQSPQGQEVMTRYGFTLPN"
     gene            complement(3177..4922)
                     /locus_tag="PKK81_08755"
     CDS             complement(3177..4922)
                     /locus_tag="PKK81_08755"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNV33007.1"
                     /translation="MSLLIRSYSSFGLSGVGLGLLLCCLSLFHSTDAISAEAGRLVEK
                     DGRYVFVESMDPAMKLLLERAVKQGTITQEEYDGVVRASQERAYLLQPSFRAWYDRGF
                     NLSMNDNAFFLKIRARFATRYTQRYRNDAYRESGDSKNFPELLGVFGDYRANRSIDQS
                     STFNLRIARLYFMGHLFSPDFRYYIQLAGETAENAQAPGALSVLDMNVTSTHLPWMNV
                     QVGQYKVYFNRSQINSTASMQFASRALVMDAFTASGINRRDVGLTIMNDEEVYPINYY
                     LGVFNGVGPLVNRFAQFNSEEATVGCPGGATGGNPFPSPAGCPTNTRNLNANLRTNVN
                     ELMYVARLQANLMGRPGYGEGDLAYSESPQMAVGGGYAYNPSIDTSSNNAFVGIDLAN
                     LHVRRSLAALGNGRMLGQGVVDFSTWTLDYVFKYRGFSLQGEYWYRNVTRHNKGLPCM
                     QTAVVGGPCTVFAPQEFGNSTGWTVQSGYYLIPRKVEVAARYAWWDPDTRSGGDLIKQ
                     VDVSLNWFLSGTYDHQIMVTYSNIAMGQDGFAIGRSAPLPAVGATFPSGTVPLDSRAG
                     TLIENAVRIQYQIFF"
     gene            complement(5028..5678)
                     /locus_tag="PKK81_08760"
     CDS             complement(5028..5678)
                     /locus_tag="PKK81_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33008.1"
                     /translation="MRVSSEHTGLLNLCMVVFGILVLGGCARLPYTKTVVHEDQRVAV
                     RLQAEVSPAGYSHPVEIPPPYIAAILRGLSLREQQRLPLRWFAEEVPPRRVFREDEVA
                     VLAPHLAAALQQVGPEERVYFEVFAPGVNPRYRRDVTGGWLAVREQYLHITMDYFHTQ
                     QPVEKSDAYDYNYPTPWTSPNTYLLYFEPGRFYLTDPKTNSRAVDFREFLKTLPPP"
     gene            complement(5718..6497)
                     /gene="modA"
                     /locus_tag="PKK81_08765"
     CDS             complement(5718..6497)
                     /gene="modA"
                     /locus_tag="PKK81_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV33009.1"
                     /translation="MGLLSLTVTPWVSAEPLVIAASPSLATPLEALARAYEAAHPDVQ
                     VKLYFDSGLDLRRMIAGMENSPTGQYFIGTGPIHLIAPGGDELITRLQQKYYVLPGSK
                     RPYATVSLMMIVPESLVEAPVSFVAMARDTRMRVAVADPDMTLLGQKTRDLFKALGIA
                     DVLNDRMDVATDARGVVDHVLNGQADVGIIFGPDAVEKADRVRVVAVASEKEGRPILH
                     SMAMERYCPNRTLCDDFLAYVQSAEAGTILKRLGYGVPTKP"
     gene            complement(6545..7735)
                     /locus_tag="PKK81_08770"
     CDS             complement(6545..7735)
                     /locus_tag="PKK81_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNV33010.1"
                     /translation="MKKAPAAEQSIQVENLLAPTRQAKGLSRIELASQAGITRQAVHA
                     IETNQYLPTTAVALRLADALGCRVEDLFRLLPQEDIVEGQWSDSATVQKTPQEAVRVK
                     VARVGARYIVRPVVDLGEVLNYAVPADGLLVPSRGVRTGAAASPRRVQVRLLRDRRVI
                     EQEIAVAGCDPSVFLAGDYLRRHQDSCTVVGWNLGSTAAIEALKRGEVHIAGVHVVDA
                     QSGESNLPYLRRHLKGNDYLVVTFAVWEEGLLVAAGNPKSVRGVEDLVRPEIRFINRE
                     AGAGARLLLDQKLIAAGMTPESVAGYSTIARSHFQVARAIAEGHADVGLGVRAAAQLF
                     GLEFLPLQQARYDLVIPHVHINDHPSIESFLNILVSRPFRTEIDALGGYDMSQTGTVH
                     ALRS"
     gene            7962..>9196
                     /locus_tag="PKK81_08775"
     CDS             7962..>9196
                     /locus_tag="PKK81_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33011.1"
                     /translation="MRAYTFFGIAVVAGSLIAGTITSVQAVEEGQLVKKDGKWKYYSG
                     EDPGLKYLFLKGIITQEEYDKGLRVLETREQIAKPNYSIDVNNGLNFRVGSKFFLKLR
                     LLTQVRYSHSAYNGGWGTVGDSRNPEILGGQVEYRAVSKQSDSNQFSVPRARLQFLGY
                     AFDPDFRYNISWAFDQTTWDQEGGSGRAGLLDAYISSWHIPWLTIQAGQQRVWFNRAT
                     ISSMATSTFADNLIVQNAFAANQQGSRDIGVSISSDEDQYKFNYAFGIWGGVGANLAR
                     EGTAVSQALPNNSSLPTAQRRTFNYDTRILTGEMMYTVRLLYKISGNPGYGQGDILNS
                     RTPQMAIAFGYAYNPAQNYLSSIRSDITDRAYRQRVTKAYNGRLLGGGIWDFQTYEAD
                     LIAKYQGWSFQAEGYYRHQR"
BASE COUNT         2068 a         2899 c         2459 g         1770 t
ORIGIN      
        1 ggaacgccgg atgacaaccc gaactcttcg accgtcgaac gagtgaccat ggcgacgagc
       61 ggaaatccac aatcgatcgt cacttgtgcc aatgccccga gtaccgtggc cgagatgacg
      121 gtcccggcca ggtgattccg cgcgctgctc gcggtggctt gcccacgttc cagcaccaca
      181 tcctccgacc ggatacacac gaacacatcg gggcctgccg attcaccttc cacggcggtc
      241 aaccgagtcc cgttcacact cacctgcacc ataccgtccg aggatccgac cacccgcccc
      301 ttcacaacgg tttcaatccc cacgacacgc gccacatccg cgttgtgggg ccggctgaac
      361 acctcgatcg gcgggcctac ctgcagcacg ttccctgcac tgatgaccgc catcacatcg
      421 ccgagggtga gggcttcggt ccaatcatgc gtgacgatga tcgacggcag cgccagctgt
      481 ttcagcagac tccgcagttc atcacgcagg tgcacccggg tcggcgcatc caaggcggaa
      541 agcggttcgt ccaacaacag caacaacggt cgaggtgcca ccgcgcgcgc aagggccacc
      601 cgttgttgtt gtccgccgga cagttcccgc ggcttcgccg tctccagtcc acgcagctgg
      661 aacagctcca ccatctcatc gacgcgcttg ttgcgatcct gggcggagag atggtggagg
      721 ccatacccga tgtttcccgc caccgtgtag gtcgggaaga gcgcatagtc ctgcgccatg
      781 tagccgatgt gacgatcttg cggcgccacc ctgacccctg acgcggtatc cagccacgtg
      841 cgcgacacaa attgaatggt gccctcttcc ggccattcca gcccggccac cgatcgaagg
      901 atcgtcgttt ttccggagcc tgacggaccg aagaggatta agacagtcga cgcctcgacc
      961 ggatatcgga tccgcgcctt gatcggcgca cgtccgggaa acgtcttcac gagttgaata
     1021 tctatttccg cggccatgct gcccacacat tacgattgac cgcatacacg aggagcaaga
     1081 tggcatatga gacgactaag agaaagagcg cggtcttggc agccccggca taatttaagg
     1141 cctgcacctc atcgtagata tcgatggaca cagtgcgcgt ctccccctcc aaattgcctc
     1201 cgaccatcaa gacgactccg aactctccca tcgtatgggc gaagctcagc acggcaccgg
     1261 tcagcaatcc ggctttagac agcggcacga tcagtttgaa gaacgtgccg agcttcgaca
     1321 cgcccagcgt ccaggaggct tcgatcaacc gccgatccac ctgctcgaac gccgcagcga
     1381 acggctgcac ggcaaacggg aggctataga gaatggaggc gagcagaagg ccttcgaagg
     1441 taaagggcag cggatgcccg atgagctcgt tgtaaaaccg tccgagcggg ctgtgcggcc
     1501 cgatggcgac aagaatataa aatcccagca ccgtcggcgg cagcaccaat gggagtgcca
     1561 ccaccgactc gacgagaaac ttccagcgcc ggcgcgaaaa gctcacccaa taggcgatcg
     1621 ggaggccgac gatcatgagg acaagcgccg tggaagcagc cagcttgaac gtcacccaaa
     1681 tcgcaatcca gttcatgatc tcgttcacga ccgatactgt tgtaccggct ctactcctac
     1741 cggatgctac cgcagacgga caggcgacag aagattattt tccgacgatg acgtcggtcg
     1801 ccttcaccgc aaccgtgacg gcatcgccga ctttgatccc catacttttg acagagcctt
     1861 cggtaatcgc cgacaccact tccaagttac cgaccttcac caccacttct gccattgcct
     1921 ggccctcggt gattcgaacg acctggcctt gaaattgatt tctcgcgctg agtttcatag
     1981 cgtcccccta gtccgtttcg gccgaggcat gtcagccgtt atgagtgcac tcattcaccg
     2041 caggatgatc catcgcgaca ctctatttcc tgcccgtagt cctatcgtcc ccgcctgact
     2101 tacattcgaa ctgaatcccc caccgccgcg ccccctcctc gccctcagcc ccctccacac
     2161 tcgacccgct gctgtaactt tgcacctgcc cgtctttcac cacctggtag ccggcagagc
     2221 atttctccgc catcaccttc agcgcctctc gtcggtacgg cgatcccatc ggaccacctc
     2281 gctcatcttt atagagataa gtcaccaccc cgccctggcc ggtttcatgg gtcaacagca
     2341 cggcctccga acaaccggcg atcagcacgg ctgcgcccag tgcaagcgca ggcatcccgc
     2401 gactaattcg gcagcgtgaa tccataacgg gtcatgacct cctgcccttg agggctttgc
     2461 ataaactgaa ggaagtgcct agccgcgtcc tgctgtttcg actgtttcat gatgaccgcg
     2521 ccctgctcca acggcggatg ggcctcagcc ggaatcagcc agtacgagcc ggcgctcttc
     2581 atgaccggcg ccatcgccag agacagggca atgatgccca catcgcaggc cccggactcg
     2641 atgaactgcg ccgcctggga gatattttct cccagcacca ggcgatcctt cacctctgca
     2701 tacacctggc tctgctccat ggccgctacc gccgcacgac catagggcgc atgcttggga
     2761 ttggcaatcg caatctttct caccgccgcg tctcgaagca ccgtcaaccc cttcgacacc
     2821 tcaaccggcg actgcttcgg cgcccacagc acgactcggc ccacggcata ccggtagagc
     2881 gaccccggca ccgtcagacc ggcctcctcg agtttcttcg gatacccgat gtccgctgaa
     2941 aagtagaggt cgaacggcgc accctgctga acctgcgcgt aaaaattccc ggacgacccc
     3001 agcgacagtt tcacatgatg accggtttgc ttctcatact ccacgatcaa ttccttgatg
     3061 gcgaactgta agtcggacgc ggcggccacc gcgatgtcat cggccaatgc gatggcgggc
     3121 cgacacaaca gcaccaacat cacatacagc agcagacgtg ccatagacga ggctccttag
     3181 aaaaagattt gatactggat gcggacggca ttttcaatca acgttccggc tcgcgaatcg
     3241 agcggcaccg tcccggaagg gaaggtcgcg cccaccgccg gcaaaggagc gctccgcccg
     3301 atggcgaacc cgtcctggcc catcgcgatg ttggaatagg tcaccatgat ttggtggtca
     3361 taggtcccgc tgagaaacca gttcagggaa acatccactt gcttgatcag atcgccgcct
     3421 gagcgagtat caggatccca ccaggcgtac cgcgcggcga cttccacttt tctcggaatc
     3481 aggtagtagc cggactgcac ggtccagccg gtcgaattgc cgaactcctg cggcgcgaaa
     3541 accgtacaag gccctcccac cacggcggtt tgcatgcagg gcagcccctt gttgtggcga
     3601 gtcacatttc tataccaata ctccccttgc agagaaaagc cgcgatattt gaacacatag
     3661 tccagggtcc aggtcgaaaa atccaccacc ccctgaccga gcattcgtcc attgcccagc
     3721 gccgccaagg accgccgcac gtgcaaattc gccagatcga ttcccacgaa ggcgttgttg
     3781 ctgctcgtat cgatcgaggg attataggca tacccgccgc ccaccgccat ttgcggactc
     3841 tccgaatagg cgagatcgcc ctcaccgtat cccggccggc ccatgagatt cgcctggagt
     3901 cgcgccacgt acatcaactc attcacattg gtccggagat tggcgttcaa gttacgcgta
     3961 ttcgtcggac accctgccgg tgacggaaag ggattgcctc cggtggcgcc gcccggacac
     4021 cccaccgtcg cctcttcgct gttgaattga gcgaaccggt tgaccagggg gcccacacca
     4081 ttaaagactc ccaggtagta gttgatcggg tagacctctt catcgttcat gatcgtgagt
     4141 cccacatccc ggcgattgat gccgctcgcg gtaaaggcat ccatgaccaa ggcacgactc
     4201 gcgaactgca tggaggcggt cgagttgatc tgcgaccggt tgaaatagac tttatactga
     4261 ccaacttgga cgttcatcca cgggagatgg gtgctcgtga cattcatatc cagcacggac
     4321 aaggcacccg gcgcctgggc attttccgcc gtttcccccg ccaattgaat gtagtatctg
     4381 aaatccgggc tgaacaggtg ccccataaaa tagagacgcg caatccgcaa attgaacgtg
     4441 gaggactggt cgatggaccg gttggcccgg taatctccga agacgcccag caattcggga
     4501 aaattcttgg agtccccgga ttcgcggtag gcgtcattcc ggtatcgttg cgtgtagcga
     4561 gtcgcaaacc tcgcacggat cttgagaaaa aacgcgttgt cgttcatcga taaattgaat
     4621 ccgcgatcgt accaggcccg aaaactcggc tgaaggagat aggcacgctc ctgtgaagcc
     4681 cgcaccaccc cgtcatattc ctcctgggtg atcgtgccct gcttcacagc ccgctccaac
     4741 agcagcttca tcgccggatc catactctcc acgaacacat atcgcccgtc tttctccacc
     4801 aaccgaccgg cctcggccga gatcgcgtcg gtcgaatgga acagactcag gcagcacagc
     4861 aggagcccca agcccacacc ggataggccg aagctggaat acgatcgaat gagtagtgac
     4921 acgggtgacg ccatcctttg catctcaggt cctccgcaca ggctctcgcc cacacaatga
     4981 tgtgatgatg gaacagggtt ccgctgaatt gaatcggccc cggtggccta cggtggcggg
     5041 agcgttttga gaaactcccg aaagtccacc gcgcgcgaat tggtcttggg gtcggtgaga
     5101 taaaagcggc ccggctcgaa gtagaggagg taggtattgg gagaagtcca gggagtggga
     5161 tagttgtagt cgtaggcgtc tgacttttcg accggttgtt gcgtatggaa gtagtccatc
     5221 gtgatatgca gatattgctc acgaacggcc agccaccctc cggtgacgtc gcgacggtac
     5281 cgggggttca cgccaggcgc gaacacctcg aagtagacac gctcttccgg ccccacttgt
     5341 tgcagcgccg ccgcaaggtg aggcgccaag actgctacct catcctcacg gaagacccgg
     5401 cgtgggggca cctcctcggc aaaccagcga agcggcaggc gctgctgctc ccgcaaagac
     5461 aatccgcgaa gaatggccgc gatatatggc ggagggatct cgaccggatg cgaatatccg
     5521 gccggcgaga cttcggcctg aagcctcacg gccacccgtt gatcttcatg cacaaccgtt
     5581 ttggtatacg gcagcctggc acatcctccc agcaccaaaa tgccgaagac caccatgcac
     5641 agattcagca accctgtatg ttcacttgat acccgcataa ccatcctcca actaccgacg
     5701 ctcacattga gcacgcgcta cggcttcgtc ggcacgccat acccgagccg cttcaaaata
     5761 gtgccggcct cagccgactg cacataggca agaaaatcgt cgcacagcgt acggttcgga
     5821 caatatcgct ccatcgccat cgaatgaaga atcggacgcc cctccttctc cgatgccacc
     5881 gcgaccactc gaactcgatc ggccttttcc actgcgtccg ggccgaaaat aatccccacg
     5941 tcggcctgcc cgttcagtac atggtccacc accccgcggg catccgtcgc cacatccatc
     6001 cggtcgttca agacgtcggc aatccccaat gccttgaaca ggtcacgggt cttttggccc
     6061 aacagcgtca tatccggatc cgccacggcc acgcgcatac gcgtatcgcg ggccatggcc
     6121 acgaaagaca cgggcgcctc gaccagcgat tcaggaacaa tcatcatcag agagacggtg
     6181 gcatagggtc gtttggaacc gggcaagaca taatattttt gctggaggcg ggtaatgagt
     6241 tcgtcgccgc ccggtgcaat gaggtggatc ggccccgtgc cgatgaaata ctgccccgtc
     6301 gggctattct ccataccggc gatcatccgg cgaagatcca acccggaatc aaaatacagc
     6361 ttcacctgta catctggatg ggcggcttca taagcccgcg ccagcgcctc aagcggtgtg
     6421 gcaagactcg gtgaagcggc gatgaccaga ggctcggcag acacccaggg tgtaacggtc
     6481 aaactcagca gacccataag gaatgcccca cataacaaca atgtttgtgg acgatgctga
     6541 cgtttcatga ccgtaatgcg tggaccgtcc cggtttgact catgtcatac ccgccgagcg
     6601 catcgatctc cgttcgaaac gggcgactga cgagaatgtt gagaaagctc tcgatactcg
     6661 gatggtcgtt gatgtgcacg tgaggaatca cgagatcgta tcgggcctgt tgcagcggaa
     6721 gaaattccag gccgaacagc tgcgccgccg cgcgaacgcc gagccccaca tcggcatgtc
     6781 cttcggcaat cgctcgcgcc acctgaaaat gggagcgggc gatcgttgag tacccggcga
     6841 cactctcggg agtcataccg gccgcaatga gcttctgatc gagaagaagc cgggcaccgg
     6901 cgccggcttc acgattgatg aatcgaatct ccggtcgtac caaatcctca acgccacgca
     6961 ccgatttggg attcccggca gctaccagga gcccctcttc ccagaccgca aaggtgacca
     7021 cgagatagtc gttccctttc aggtggcggc gcaggtaggg caaattagac tctccggact
     7081 gcgcatccac gacatgcacg cccgcaatgt gcacctctcc ccgtttgagc gcttcaattg
     7141 ccgcggtact gccgagattc catcccacca cagtgcaact gtcctgatgc cgccggagat
     7201 aatcaccggc cagaaagacc gaggggtcac aaccagccac ggctatctcc tgctcaatga
     7261 cacggcggtc ccgaagcagg cggacctgca cgcgacgcgg cgaagcggcg gctcctgtcc
     7321 ggaccccacg ggagggaacc agaaggccgt ctgcggggac cgcataattg agcacctctc
     7381 ccagatcgac gacgggtcgg acaatgtatc gcgcgcccac ccgcgcgacc ttgacgcgaa
     7441 cggcttcctg cggcgtcttc tgaaccgttg cggagtccga ccattgaccc tcaacgatgt
     7501 cttcttgagg aagcagccgg aagagatctt ccacgcggca gcccagagca tccgcaaggc
     7561 ggagcgctac ggctgtagtg ggaaggtatt gattcgtttc gatggcatgg acggcttgcc
     7621 gtgtaatacc ggcttgcgag gcaagctcga tgcgtgacaa gcctttggct tggcgtgtcg
     7681 gggccaagag attctctacc tgaatggact gttcagcagc gggcgccttc ttcatgacgg
     7741 gacgaaaaat gacataatgc tttacctacg tcaagcattg attccagcaa ggagaagtat
     7801 ttcatgggca cataaatttt aatcataatt gttgacaatc ttacataact tgatgtattt
     7861 aattcctcac cggaatatag atatttaata aattctatat gaattaacat tttacaagat
     7921 atcaacggtt ctgaatatca cccactcgga ggttagatca gatgagagcc tacacttttt
     7981 ttggaattgc cgtggtggcc ggcagcttga tcgctggaac catcacctcc gttcaagcgg
     8041 tcgaggaagg ccagctggtt aagaaggatg ggaagtggaa gtactattca ggcgaagacc
     8101 cggggctcaa ataccttttc ttaaaaggca tcatcaccca ggaagaatac gataagggcc
     8161 tgagagtcct ggagacccgg gaacagattg ccaaacccaa ctacagcatc gacgtcaaca
     8221 acgggctgaa cttccgcgtg gggtcgaaat tctttttgaa actgcgcttg ctcacccagg
     8281 ttcggtacag ccacagcgcc tacaatgggg gatggggcac cgtcggcgat tcgagaaatc
     8341 ccgagatcct cggcggtcag gtggaatacc gagccgtcag taagcaaagc gactccaacc
     8401 agttcagcgt gccgcgcgcc cgccttcagt tcctgggcta cgccttcgat cctgactttc
     8461 gctacaacat ttcctgggcc ttcgatcaga cgacatggga tcaagaggga ggcagcggac
     8521 gtgcgggcct cctcgacgcc tacatctcgt cctggcacat cccctggtta accatccagg
     8581 caggtcagca gcgcgtctgg ttcaatcgcg ccacgatcag ttccatggca acctcaacat
     8641 tcgcggacaa tctgattgtc cagaacgcgt ttgcagccaa tcaacaggga agccgcgata
     8701 tcggcgtcag catttccagc gatgaagacc agtataaatt caactatgcc ttcggaatct
     8761 gggggggagt cggtgccaac ctggcacgag aaggcacggc cgtcagccaa gcgcttccca
     8821 acaattcaag ccttccgacc gcccagcgcc gtacattcaa ttacgacacc cgcattctga
     8881 cgggcgagat gatgtatacg gtccgtctgc tgtacaaaat ttccggcaat cccgggtatg
     8941 gccagggaga tattctgaat tcacgcacgc cgcagatggc catcgccttc ggctatgctt
     9001 acaaccctgc ccaaaactac ctgagttcga tccgctcgga tattaccgat cgcgcctacc
     9061 gtcaacgtgt cacgaaagcg tacaacggac gtctgctcgg cggtggcatc tgggattttc
     9121 aaacctatga agccgatctt atcgcgaagt atcagggctg gtccttccag gccgaaggat
     9181 actatcgcca ccaacg
//
LOCUS       DAOQRM010000098         8817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_7401_length_8817_cov_16.902534, whole genome shotgun sequence.
ACCESSION   DAOQRM010000098 DAOQRM010000000
VERSION     DAOQRM010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7401_length_8817_cov_16.902534"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1566)
                     /locus_tag="PKK81_08780"
     CDS             complement(172..1566)
                     /locus_tag="PKK81_08780"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV33012.1"
                     /translation="MRVLLVEDHIDIRRLFEQVIEARGHDVTACADGESAWEAYSRRP
                     YELVLLDWELRGGGMDGLQVCRTIRSSPGGDRCVIVMITGHDSPESLRTALQAGVNDY
                     LVKPVGVEFLKLRLTIAEQWVESVRRRFAAEDQAQALQSQLADHGKFHDLIGRSPAMV
                     VLYEEIQKIAAVDATVLIEGETGTGKELVARAIHLSSRRASHTFLAVNCAGFTDSILG
                     SQLFGHKKGAFTGAIDNQEGVFEAAQGGTIFLDEIGDISPAVQTSLLRVLQEREVTRL
                     GESKPRKVDVRVVVATHHNLAEDVEKGTFRRDLLYRIKVARLQLAPLRERPTDIPLLV
                     HAFLGQFRSVMEKAVLQVSPEALQVLINHSWPGNVRELKSAVESALIHCKGTVVRVED
                     LPPEVRNPEPAATYVHFKRQDERTRMVGALQQTGGNRSEAARLLGMSRRTFYRRLAEY
                     DVSALGDGGDAPLQ"
     gene            complement(1581..>5285)
                     /locus_tag="PKK81_08785"
     CDS             complement(1581..>5285)
                     /locus_tag="PKK81_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV33013.1"
                     /translation="LELRVAEQTKNLVSANGRLTAQLAEQRRIEAELRESRERFELAA
                     EGADVGVWEWDLQEHTAYYSPRWKSQLGCAESEIGATVADWEARLHPDDRARALATVT
                     LFQEGYTKQYRLDHRLRHHDGSYRWISSLGAGVFDEQGRMIRMTGIHLDVTARTQAEE
                     ARRESEDRYRALVEQAGDIIYRTDAAGRFTYCNPTSLRVLGYLPEELLGRHYLEIVLP
                     QCRPQAERFYGRQFIRKTPRTVYEVPVATKDGREVWLGQHTQLLLEGGVVSGFQVVAR
                     DITERKGVEQALRESEERFSKAFHSSPAGMSISRLEDGRVLDVNEAFVRVSGFSREEL
                     IGMSSLDIGIWVNPEDRRHLAEILRRDGFLRHLEKEFRTKSGEVRHGLFNVEPVCIGR
                     ETCVLTLVLDITRRTEAEAALRRSQAVLQAHQHALMRLTKSQHIGSGDWQSALEELMQ
                     TSALVLAVDRASVWLLDRTGTILDCVELYEQGQARHSRGQRLNVVEYPRYFAELLQEQ
                     VVDAHDVAADPRTSELVQPYLGALGIASLLDVPIFFGGRLAGVVCHERVGVPREWTRE
                     EVQFATSVGNLVTLAYEAKQRLEAENALMTAKEAAEFANRAKSDFLATMSHEIRTPMN
                     AIVGMADLLSETPLNEDQREYVQIFRDAGSNLLSLINDVLDLSKIEAGHLDLDLVDFD
                     LNDLVQRAAELVAVRASEKNLELAYQIQPDVPTSLVGDPNRLRQVLLNLLGNAIKFTD
                     EGEVVLRVERDPQADGPGTILFTVRDTGIGIPTDKLTAIFERFTQVDSSTTRPYSGTG
                     LGLTISRRLVERMGGKMWVDSELGRGSTFSFTAKFAVHVQPAPAVPPAQWEQLASLRT
                     LIVDDNATNRLIVRETLAGWGIPAAEVPGGEEALFELRRASKAGVPYRLVILDVRMPK
                     LSGWQVAETVARTPGLAGVSIIMLTSERRAGDQARARECGVVRYLTKPFRRSDLFNGM
                     MAVIGKVALAEGNALLSQPPDSTGDMPGLKVLLAEDFVDNRRMMEFYFKSTPHRVETA
                     ANGQLAVEMFQRGSYDLVLMDIQMPVLDGYAATRAIRSWEQAQGRSPVPILALTANAL
                     QSEVQRSLEAGCTTHLTKPIRKARLLEAIQLYFQTSVPVAPSSSDASADTVVLQVSGE
                     FESLMPGFLEHRRQELIQIADALDRGDFETIREIGHGLKGAGGTYGLEAISAYGRALE
                     TAAGQKDGVCVREALHRLTRFLERLQLVYV"
     assembly_gap    5286..5385
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5627..6472)
                     /locus_tag="PKK81_08790"
     CDS             complement(5627..6472)
                     /locus_tag="PKK81_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HNV33014.1"
                     /translation="MMISQDGVLDRGSVAVVVPAYNRAQFTPDEEISFRHLEHVLGRY
                     DKFLAVPRSLEIERPGYQIQRFDDGYFGSAIANGKLMLSPAFYESFRKYRYILIYQLD
                     ALVFSDQLLEWCATDVDYIGAPWVQCADSPWVGTPRVGNGGLSLRKVSSFLRVLSSEQ
                     YWIHPDVYWQRVVSGTPWYLRGVYLPRKWFKYIKRFNGVKRQVAQWHLRPDGTKNEDH
                     FWSDEAVRYDDQFKVASFDMGLRFAFEVVPRHCFELNNRRLPFGCHAWPRYDRAFWEP
                     YLLKS"
     gene            complement(6842..7942)
                     /locus_tag="PKK81_08795"
     CDS             complement(6842..7942)
                     /locus_tag="PKK81_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV33015.1"
                     /translation="MTKLRVGVIGAGAFAESCHVPGVQSHPEAEVVVLCGRDSARTRA
                     AAQRLGVPEVSLDYEEVCARPDIDAVTIATPNVVHARQAQAAFAAGKHVFCEKPLGMN
                     TEEAADMLRAAEQSGKIHQTAFTYRYLYGVQELKRRLLNGEIGEPYYVAVHYDSWDGL
                     RPGSRIGFREHLDSAGAGVLYDVGSHLFDLVGFVLGPIEAVSGNTILVPRKRIDARTG
                     ALAHVETDDIASAAFTCRGGIQGQLFASRATPNSGDKAYIELVGRQGALKASLSRGSV
                     DVLTISRPSQPGWEVVPLPVQASDGQPHCLSRMMHSFVEACLRGSLNGEIDASFHDGL
                     AVQRAMDAVRQSSAEPAAISLKAAFDPSPDYS"
     gene            complement(8191..>8817)
                     /locus_tag="PKK81_08800"
     CDS             complement(8191..>8817)
                     /locus_tag="PKK81_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV33016.1"
                     /translation="ATAGVIIGASPAITALLSSLLVRDIPFNTVAAGCAVSFAGVALV
                     SGVGGEAPSGDNPWLGGTLVLLGLVSWALYSIGGRQVMERLSPLTVNWTTLLLSLLLQ
                     IPLLWTDQKLLLTGASVVPVSGWVALGYLIVFATALGQQAWLYGVQGVGPSRAGVFVN
                     LIPVSALLLSALVLGEAIGVREIVGIALILAGVWLVGWQSARLKHAGG"
BASE COUNT         1862 a         2919 c         2338 g         1598 t
ORIGIN      
        1 tgcctcgatg cacagtctca acacatgcac ccgcgcgcga ggctgaagcc gaatctgatg
       61 cgcacggaat ggcgcgtcag gcgaagaaag taggaaatgg tggattcaga ggaaagatct
      121 atctgacgcg gaggtacagg tccccgcatt tagatcgcaa tcgaggcgga ttcactgaag
      181 cggagcatcc cctccgtcac ccagtgcgga cacatcgtat tcagccaggc ggcgatagaa
      241 ggttcgccgg ctcatcccta acaaccgtgc tgcctccgag cgattgccgc ccgtttgttg
      301 cagcgccccg accatgcgag tccgttcatc ctggcgttta aaatgcacat aggtcgccgc
      361 cggctccgga ttccggacct ccggcggaag atcctccacc cgtaccacgg tccccttaca
      421 atgaatcagg gccgactcca ctgcactctt gagttctcgc acattgccgg gccacgaatg
      481 gttgatgagg acctgcagcg cttccggact cacctgtaac acagccttct ccatcacgga
      541 gcggaactgt ccgaggaagg catggaccag taacggaatg tcggtgggcc gttcccggag
      601 cggggcgagt tggagtcgag ccaccttgat gcgatacaac aggtcccgtc tgaacgtgcc
      661 tttttccaca tcttcggcga gattgtggtg tgtcgccacc accacccgca catccacctt
      721 gcgcggcttg gattctccca accgggtcac ttcacgttcc tgcagcacac gcaggaggct
      781 ggtctgcacg gcgggagaaa tatcgccgat ctcatctaga aatatggtgc ccccctgcgc
      841 agcctcaaac acgccttcct gattatcgat cgcccccgtg aaggccccct tcttatgacc
      901 gaagagctgg ctgcccagaa tcgagtcggt gaacccggcg caattgacgg ccagaaaagt
      961 gtgggacgcg cggcggctgg agagatgaat tgcgcgggcc accaattctt ttcccgtacc
     1021 ggtttcgcct tcgatcaaca cggtcgcatc gacggcggcg atcttctgga tctcttcgta
     1081 gagcaccacc atcgcgggac tccggccgat caagtcatgg aacttgccgt gatcggccaa
     1141 ctgcgactgc aaggcctgcg cttgatcttc cgccgcgaag cgccgtcgca cactctccac
     1201 ccactgctcc gcgatcgtca accgcagctt caagaactcc acaccgacgg gcttcaccag
     1261 atagtcgttg acccctgcct ggagcgccgt acgcaaggat tccggggagt catgcccggt
     1321 gatcatgaca atgacacagc ggtccccgcc gggactcgat cggatggtcc ggcacacctg
     1381 caggccgtcc attccgcccc cccgcaactc ccaatcgagc agcacgagtt catagggtcg
     1441 ccgactgtag gcctcccagg ccgactcgcc gtccgcgcaa gccgtgacgt catgtccccg
     1501 cgcctcaatg acctgttcga acaggcgccg gatatcgata tgatcctcca ccagcaagac
     1561 acgcatggct tgcttactcc ttacacatag accagttgca gccgctccag aaaacgggtg
     1621 agcctgtgga gcgcctcgcg aacgcacacg ccgtccttct gacccgccgc cgtctccaag
     1681 gctcgtccat aggcgctgat tgcttcgagg ccataggtcc ccccggcccc cttaaggcca
     1741 tgcccaatct cgcgaatcgt ctcaaaatct cctctatcca atgcgtccgc aatctggatg
     1801 agttcctgcc gtcgatgctc taggaacccc ggcatcaggg attcaaactc tccgctcacc
     1861 tgcaacacga cggtgtcggc tgacgcatcc gacgacgacg gcgcgaccgg aacggaagtt
     1921 tggaaataca gttgtatggc ttccagcagg cgggcctttc ggataggttt cgtcaaatgc
     1981 gtcgtacacc ccgcctccag actccgctgg acttcactct gcaacgcatt ggccgtcagc
     2041 gccagaatcg gtaccggact ccggccctgc gcctgctccc acgaccgaat cgccctcgtc
     2101 gcggcatacc catccagcac gggcatctga atatccatca gaaccagatc gtacgagccc
     2161 cgctggaaca tctcgacggc aagttggccg ttggccgcgg tctcaacccg atgaggcgtc
     2221 gatttaaaat agaactccat catgcggcgg ttgtcgacaa agtcttcggc caacaagacc
     2281 ttcagccccg gcatgtcccc ggtcgaatcc ggcggctgag acagcagcgc attgccctca
     2341 gccaacgcca ccttgccgat caccgccatc ataccgttga acaaatccga tcgtcgaaac
     2401 ggtttcgtga gatagcgcac caccccgcac tcacgggctc gcgcctggtc accggcgcgc
     2461 cgctccgagg tcaacatgat aatggatact cctgccaggc ccggagtacg tgcaaccgtc
     2521 tccgcgacct gccacccgct gagtttcggc atgcgcacat ccaagatcac caaccgatac
     2581 ggcactccgg ccttcgacgc gcgacgcagc tcaaacaggg cctcctcgcc accagggacc
     2641 tcggccgcgg gaatgcccca tcccgccagc gtttcccgca caatcaggcg gttggtcgca
     2701 ttgtcgtcca cgatcaaggt ccgcaagctc gccaattgct cccactgcgc cggcgggacc
     2761 gccggcgccg gctgaacatg cacagcaaac ttggccgtga agctgaacgt actccctctg
     2821 cccagttcgc tgtccaccca catcttgccg cccatccgct caaccagtcg tcgggaaatc
     2881 gtcaacccga gcccggtgcc gctgtacggc cgggtcgttg aggaatccac ctgggtaaat
     2941 cgttcgaaga tggcagtcaa cttgtctgtg ggaattccga tgccggtatc ccgcaccgta
     3001 aacaggatgg tcccggggcc gtccgcctgc gggtcccgtt ccacacgcaa caccacttcg
     3061 ccttcatcgg tgaacttgat ggcattgccc aacaggttga gcaacacctg ccggagacga
     3121 ttcggatcgc ccaccagcga cgtcggcaca tccggctgaa tctggtaggc caactccagg
     3181 tttttctcgc tggcgcgcac ggcaaccagt tcagccgccc gttgcaccag gtcgttgaga
     3241 tcgaagtcca ccaaatccag atccaagtgg ccggcttcga tttttgacag atccagcaca
     3301 tcgttgatca ggctgagcag gttgctcccg gcatcgcgaa aaatctgcac atactcccgc
     3361 tgatcctcgt tcaagggtgt ttccgacaac agatccgcca tgccgacgat cgcattcatc
     3421 ggcgtcctga tctcgtggct catggtcgcc aggaagtcgc tcttggcccg gttcgcgaac
     3481 tcggccgcct ccttcgccgt catgagcgca ttttcagcct ccaaccgttg tttcgcttcg
     3541 taggccaggg tcaccagatt cccgacggac gtggcgaact gcacttcttc tcgcgtccac
     3601 tcgcgcggca caccaacccg ctcatgacag accacgccgg ccaatcgacc gccgaagaaa
     3661 atgggcacgt ccaacaacga agcgatcccg agagccccga gatagggctg caccaattcg
     3721 ctcgtgcgcg gatcagcggc aacatcatgt gcatcgacca cttgctcctg gagcaattcg
     3781 gcgaagtagc gagggtactc gaccacattc aatcgttgcc cccgtgaatg ccgcgcctga
     3841 ccttgctcat acagttccac acagtccagt atggttccgg ttcgatccag caaccagaca
     3901 ctcgcccgat ccacggccag caccagtgcc gacgtctgca tcagttcttc cagcgccgac
     3961 tgccagtctc ccgacccgat atgctggctt tttgtcaacc gcatcaacgc atgttggtga
     4021 gcctgcagca ctgcttgact gcgacgcaac gccgcctccg cttccgtgcg tctcgtgatg
     4081 tccagcacga gggtgagcac gcaggtctcc cggccgatgc agaccggctc gacattgaac
     4141 agcccgtgcc gcacctcgcc ggatttcgtg cggaactctt tttccagatg tcgcaaaaac
     4201 ccgtctcgcc tcaatatctc ggccaagtgc cggcgatcct ccggattcac ccagattccg
     4261 atgtcgagcg aggacatgcc gatcagctct tcacgggaaa atccggacac ccgcacgaac
     4321 gcctcattca cgtcgagcac ccggccgtcc tcaagtcggc tgatcgacat cccggccgga
     4381 ctgctgtgaa acgccttgga aaagcgctcc tcgctttcgc gcaacgcctg ctcgacacct
     4441 ttccgctcgg tgatatcccg ggccaccact tgaaagccgc tgacgacgcc cccctccagt
     4501 aacagctgcg tatgttgccc caaccacact tctcgaccgt ccttggtagc caccggcacc
     4561 tcatagacgg tacggggcgt tttacggata aactggcggc catagaaccg ctcggcttgc
     4621 ggacggcact gcggcagcac gatctctaag taatgccgac cgagcaactc ctccggaaga
     4681 tagcccagga cacgaagcga ggtcgggtta cagtaggtga acctcccggc ggcatcggtc
     4741 cgatagatga tatcgccggc ctgctccacc aacgcgcggt atcggtcttc gctttctcgt
     4801 ctcgcctcct cagcctgtgt cctcgccgtg acatccaggt gaatgccggt catccggatc
     4861 atccgcccct gctcatcaaa tactcccgcg cccagcgaag agatccagcg ataggacccg
     4921 tcgtgatgcc ggaggcgatg gtccagacga tactgtttgg tatatccttc ctgaaacaac
     4981 gtgaccgtcg ccagcgcccg cgcgcgatcg tcagggtgaa gacgcgcctc ccagtcggcg
     5041 accgtcgccc cgatttccga ttcggcgcat ccaagctggc ttttccagcg cggggaatag
     5101 taggcggtgt gttcctgcag atcccactcc cacaccccaa catccgcacc ttccgcggcc
     5161 agttcgaacc gctcccgact ctcgcgcagc tcggcctcta tccgccgctg ctcagccaat
     5221 tgagccgtca gccggccgtt ggcggaaacc agattcttcg tctgctccgc cactcgcagc
     5281 tccagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtaag atacgcccat
     5401 gactcactga ggtaaacccc ggcaatcggc cctagtacgg ccctactcca tggagcagtg
     5461 atcagaagag gctgaaagag agaccgtcac gttcacgacg gacagacacg caatcgcatc
     5521 atgtgagaca aggagaaggg aggagggcta tgagtcgagc gactcctgaa tgtggccccg
     5581 aatcacggag cgggcttgcc tgacagaccg atgcagcggg acagaactac gacttgagga
     5641 gatagggctc ccagaaggcg cgatcatacc gcggccaggc atggcaaccg aaaggtagtc
     5701 ggcgattgtt gagctcaaag caatgcctgg gaacaacctc aaaggcgaac cgaagtccca
     5761 tgtcgaacga ggcgacctta aactgatcgt catatcgcac cgcctcgtcg gaccagaaat
     5821 gatcttcatt tttggttcca tcgggacgta gatgccattg cgcgacttgt cgcttcacgc
     5881 cattgaatcg tttgatatat ttgaaccact tccttgggag atacacacct cgcaggtacc
     5941 agggcgtccc ggagacgact cgctgccaat acacgtcagg gtggatccaa tactgttccg
     6001 acgacagcac ccgtaggaag cttgagactt ttcggagaga caacccgccg tttccgacac
     6061 gcggcgtccc cacccagggg ctgtcggcac attgcaccca cggtgcgccg atataatcga
     6121 catcagtggc gcaccattcc aacaactgat ccgaaaacac cagggcatcg agctgataaa
     6181 tgagaatata acgatacttc cggaacgact cgtaaaaggc aggcgacagc atcagtttcc
     6241 catttgcaat cgcactcccg aaatagccgt cgtcgaaccg ttgaatctgg tagcccggcc
     6301 gctcaatctc cagactccgc ggcaccgcca gaaatttgtc gtaccggccg agaacatgct
     6361 ccagatggcg aaacgagatt tcttcgtccg gggtaaattg cgccctgtta taggcgggca
     6421 ccaccaccgc gacgcttcct ctgtcgagca caccgtcctg cgatatcatc atccgccctc
     6481 acctcgccta cgtggcaata tcagcatcag ccgcttcgat cataccgtgc agttccatcg
     6541 tggtcgtggt cttgcccctc tcaggaccga cgggcgactg atgcatgcgc agtgacaggc
     6601 cgcaccctgt ccgagccagg ccgcgcgacg gcccgcacca catgaataat cggccaggtt
     6661 atgccactcc gtcgaaggcc ccagtccgga ctattcatga atgccgtcgc gagacgttcg
     6721 agacggaagc ccgccgaggc tgctcgcacg taaggccgac gcaggcgtac tggcagtccg
     6781 tcgaggaggc cgaacgagaa cagcctcgcc gagcgggagg atcggacgcc ggagcaggta
     6841 ttcatgaata gtccgggcta ggatcgaaag cggccttcag ggagattgca gcgggttcgg
     6901 ctgaggattg tcgcaccgca tccatggcgc gctgcacggc gagtccgtca tgaaaggacg
     6961 catcgatctc cccattcaaa cttccgcgaa gacaggcctc gacaaaactg tgcatcatgc
     7021 gggaaagaca atgaggttgg ccatccgagg cctggaccgg caacggcacg acttcccagc
     7081 cgggttgcga cgggcgtgag attgtcaaga catccaccga cccacggctc aatgaagcct
     7141 tcagggctcc ctgtcgcccg accaactcga tgtacgcttt gtcgccggaa ttcggggtcg
     7201 cgcgactcgc aaacaactgt ccctgaatcc cacctcgaca ggtaaacgcc gccgatgcga
     7261 tgtcgtcggt ttccacatga gcgagcgcgc cggttctcgc atcgatccgt ttccgtggaa
     7321 ccagaatggt atttcctgaa acggcctcga tcggcccgag cacaaatccg acaaggtcaa
     7381 agaggtggga cccaacgtcg tacaataccc cggctccggc ggaatcgaga tgttcgcgaa
     7441 agccaatcct cgaaccaggt ctcaggccgt cccaggaatc atagtgcacc gccacataat
     7501 agggctcgcc gatctccccg ttcaaaagcc ggcgcttcag ttcctgcaca ccgtacaagt
     7561 agcgatacgt gaacgcagtc tggtgaatct tgccgctctg ctccgcggcc cgaagcatgt
     7621 cagctgcttc ctccgtgttc atcccgaggg gtttttcaca gaacacgtgc ttgccggcag
     7681 caaatgcggc ctgggcctga cgggcgtgca ctacattcgg ggttgcaatc gtgactgcat
     7741 cgatgtcagg ccgtgcgcaa acctcctcat agtcgagcga tacttccggc acacccaacc
     7801 gctgcgccgc cgcgcgggtc cgtgccgaat ctcgcccaca gaggaccaca acctccgctt
     7861 ccggatgcga ctgcacgccg ggcacatgac agctttcggc aaacgctccg gccccgataa
     7921 cgcccactct taatttggtc attccgccac cttgcaaaaa atgtcacatc tgacgcgacg
     7981 gaatcagatc ccctcgcgac aggtcggcga caaccgtgta cggtatgctc acacaggatg
     8041 agagccatgg caggcatctg ggctcactgg ttcactctag cggattaccg gaggcgtgtc
     8101 gagactgctg ctttatggga gtgagaactg cgtagatgaa ccgatgacac cgcaaacgca
     8161 tgctgtacct gtttatgcgg cgggaagaag ctatccgccg gcgtgtttga gtcgggccga
     8221 ttgccaaccg accaaccaga caccggccag gatgagcgca atgcccacga tctcgcggac
     8281 gccgatcgcc tcacccaaca ccagcgcaga cagaagcaga gccgataccg ggatgagatt
     8341 cacgaacacg ccggcccgcg aaggacccac cccttgcacc ccgtacaacc aggcctgctg
     8401 ccccaatgcc gtagcgaaca cgatcagata ccctaaagcc acccacccgg agaccgggac
     8461 cacactcgct ccggtcaaca gcaacttctg atcggtccac agcagaggaa tctgcaacaa
     8521 gagggacagc agcagcgttg tccagttgac ggtgagcgga gagagccgct ccatcacctg
     8581 gcgaccaccg atggaataca acgcccaact caccaatccc aacaacacca gtgtgccgcc
     8641 cagccaggga ttgtccccgc tcggcgcctc ccctccgaca ccggacacga gtgcgactcc
     8701 ggcaaaggag accgcacaac cggccgccac cgtgttgaat ggaatgtctc gcacgagaag
     8761 agacgacagc aacgccgtaa tggccgggct ggcgccgata atgacgccgg cggtcgc
//
LOCUS       DAOQRM010000099         8809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_7410_length_8809_cov_15.881426, whole genome shotgun sequence.
ACCESSION   DAOQRM010000099 DAOQRM010000000
VERSION     DAOQRM010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7410_length_8809_cov_15.881426"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKK81_08805"
     CDS             complement(<1..410)
                     /locus_tag="PKK81_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HNV33017.1"
                     /translation="MGDDLIKYLGPLAALAGVWEGDKGADVAPDDNRGTEQNAYRERL
                     TFEPFGPVNNHEQQLYGLRYATVAWRLSDGAPFHEETGYWLWDAAAKQVLRCFIVPRG
                     VTVLAGGTVEPGAVSFQISADAGSDTYGICSNKFL"
     gene            complement(526..876)
                     /gene="ssrA"
                     /locus_tag="PKK81_08810"
     tmRNA           complement(526..876)
                     /gene="ssrA"
                     /locus_tag="PKK81_08810"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1071..1685)
                     /locus_tag="PKK81_08815"
     CDS             complement(1071..1685)
                     /locus_tag="PKK81_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33018.1"
                     /translation="MTRLVVLMIAGTVLMSSGREAGAWPKMSTTVSEAMVWLTKGSSR
                     SKPAPLKYKVVGVFQRNKQFVGGSAVADVTKQVEFEFKWPLEDGVSPKVLPDIPTKAE
                     HYRDGSAVCPAPILKGAYEHFELKSILVMGPMFGLVGERRTPDMEEYVGGGGCKGTRL
                     VQGQVREEQVLVTLPPEFLAGQLSKGQVFTQQDQGWTWTFTPVP"
     gene            complement(1932..2882)
                     /locus_tag="PKK81_08820"
     CDS             complement(1932..2882)
                     /locus_tag="PKK81_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HNV33019.1"
                     /translation="MNTFQFLADHGASVLFWVIFVEQIGFPIPAIPLLIAAGALVGAG
                     KMSLAMALLVPVAAALPPDLAWYYLGRIKGGKVLGFLCRLSLEPDSCVRDTENLFHRY
                     GPRGLLLAKFIPGFNTVAPPLAGIVGMAATTFALYDVAGTLIWAAVSAGVGTLFSNQL
                     EQLVGLLDQAGGLVFGVLLTGLTGFIGYKFYYRQQFLRHLRMSKISVDELKQRLDAGE
                     AISVVDVRHPLSLQLDPDTIPGAINFTLEEIEHRHQEIPRDRDIVLYCTCPNEVSSAR
                     TAFLLKKKGIHRVRPLEGGLDAWRERQYPVERRVNAGVGG"
     gene            complement(2903..>3733)
                     /locus_tag="PKK81_08825"
     CDS             complement(2903..>3733)
                     /locus_tag="PKK81_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33020.1"
                     /translation="PNWWILGGSLAFVLFTLTVGVADFAYSEEIVFAGSMAIVSLLMV
                     RLTRELAPDARHVLVGTALVVFVFRAVPGPGEGATWWMIDQLKFDQQFLSVLSLIGSA
                     LTLAGMFLFRRFMAERSIAYVVGFLTLAAFFLGLPIVSMYYGLHEWTAAVTGGLVDAR
                     FIALVNTALESPLGQISMIPMLAWIANSAPANLKATYFAVMASFTNLALSLGQLGTKY
                     LNELFVVTREVRDPVTNAIQTPDDYSQLGLLLIVQALLGLALPFAAILFARFSRYRSA
                     "
     assembly_gap    3734..3833
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3834..4372)
                     /locus_tag="PKK81_08830"
     CDS             complement(<3834..4372)
                     /locus_tag="PKK81_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33021.1"
                     /translation="MIAAASRWIDRNILELARDFQLSYLPPLMVYMAAGISGLTGIVG
                     TFFVKDYLGLSAAFLAALGFWAGIPWALKMPFGHLVDLLWRWKSLLVYFGAGVIAASL
                     LIMVGLIGHREAMVAVMPAEVWYVLSVLLSPIGYVIQDTVADAMTVEAVPRVDERGVP
                     IDEARLKLMHTTMQTLGRVA"
     gene            complement(4518..5591)
                     /locus_tag="PKK81_08835"
     CDS             complement(4518..5591)
                     /locus_tag="PKK81_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNV33022.1"
                     /translation="MGKMSIKWMVGTVVVATVGAMGLAATRVMGEPSVAQPGAVPVAQ
                     EVPLGLEDPSGYIPADNPQTARKIELGRWLFFDKRLSKNGTVACATCHMPGLAFTDGQ
                     PVSTGIYRLQGGRSAPTAINRVYSKAQFWDGRADTLEDQSVGPFVSPVEHGFLDHNEL
                     VSKIKSIEDYRMLFREVFGREVTVGDVGRAITAFQRTLLSGNSPVDRFDMGGDEQALS
                     DSAKRGLELFRGKARCTRCHSGFNFSDEKFHNLGIGWDTNTVDLGRYMVTKNAEDIGA
                     FKTPTLREIARTAPYMHDGRFATLEDVVRFYNQGGIKNPHLDNTVIPLELTGPEQQDL
                     VAFLRSLNGEGWQHVAAPVEFPK"
     gene            complement(5639..5845)
                     /locus_tag="PKK81_08840"
     CDS             complement(5639..5845)
                     /locus_tag="PKK81_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33023.1"
                     /translation="MRQIVIAVGMGFAVIMLQACATPSRPTGHQTVAFSEGQQTPLSE
                     EEFLQKVERHMERQDRPGTTLRFR"
     gene            5978..6892
                     /locus_tag="PKK81_08845"
     CDS             5978..6892
                     /locus_tag="PKK81_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HNV33024.1"
                     /translation="MTLTELQYIVAVSRERHFGRAADRVFVTQPALSLAIKKLEDELG
                     VAIFERRKNHVELTPLGEQIVHQAQRVLEEADQIKLLAAQGKNQLVGALRFGVIATVG
                     PYLLPDLIPLLHKRAPGMPLEIEENLTVNLTAMLKSGKLDVIMIALPFEEPGILTQAL
                     YDEPFKAVVPADHRLAKRSKIDSALLSTERVLLPHAGHCFRQQVLESCPELSRSDTES
                     LQGNSLETIRQMVASGLGITVMPCSAVTAKHQNKRLAVLEFMKPVPERRIALAWRKGF
                     TRPAVIPVIQEAVGALKIPGLMAIASGQ"
     gene            6918..7268
                     /gene="uraH"
                     /locus_tag="PKK81_08850"
     CDS             6918..7268
                     /gene="uraH"
                     /locus_tag="PKK81_08850"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HNV33025.1"
                     /translation="MAESRISTHVLDIAQGRPAQGIPVTLEGQGLAGIWKLLGKSRTD
                     ADGRSNDLYPAKQRLQPGVYRLTFDVSTYFRSQHVTSFYPEVVVIFTLSDAGQPYHIP
                     LLLSPFGYSTYRGS"
     gene            complement(7296..7934)
                     /locus_tag="PKK81_08855"
     CDS             complement(7296..7934)
                     /locus_tag="PKK81_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV33026.1"
                     /translation="MASVSLAGAEPGHRTGDQALIAAAERNDVEAVRQLLKEGASVRA
                     RDPQGRTALLAATYRNHVEIAKLLILTGSDVNTQDAMQNSPLLLAGASGYLDILRMTL
                     AADPDFTIYNRYGGTALIPACERGHVEVVKELLKTKIDVNHVNRLGWTALLEAIILGD
                     GGPTHQTIVGLLVAAGADLNLGDGDGVTPLRHARQKGYRVIADILLSAGARE"
     gene            complement(7988..>8809)
                     /locus_tag="PKK81_08860"
     CDS             complement(7988..>8809)
                     /locus_tag="PKK81_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV33027.1"
                     /translation="NFYVDSTCINCDACRQLAPSSFEEVGEYSAVTHQPATEPERRQA
                     YRALLACPTGSIGAEHTNQTLLSAAKADFPLPIEGNVYYCGFNSEKSFGANSFFVQHP
                     DGNWLIDSPRYLKPLVDVFDRMGGLAQIFLTHEDDVADAARYARRFGARRIIHRADVH
                     AMPEAEVIIEGPEPRSYSEDFRIIPVPGHTAGSLCLLHRDRFLFTGDHLWWDPEAREL
                     ASPQQLVWNAEALHRSIEQLTNCSFEWVLAGHGGRVWLPQSEMRAAVRELVERRQ"
BASE COUNT         1835 a         2813 c         2424 g         1637 t
ORIGIN      
        1 agaaacttat tcgaacagat gccatacgta tccgatccgg catccgccga aatctgaaac
       61 gagacggcgc ccggctccac agttccgccc gcgagcaccg tgacaccgcg cggcacgatg
      121 aaacagcgca gcacctgctt cgccgccgca tcccacagcc agtatccggt ttcttcatga
      181 aacggcgccc catcgctcaa ccgccaggcc acggtcgcat aacgaagtcc gtaaagctgt
      241 tgttcatgat tgttgaccgg accgaacggc tcgaaggtca accgctcgcg ataggcattc
      301 tgttccgtgc cccggttgtc atcaggcgcc acatcggcgc ccttatcgcc ttcccacaca
      361 ccggccagcg ccgccaacgg accgagatac ttgatcagat cgtcacccat gtcatcgttc
      421 ctttcatgcg gttgcctgtt ccatcggtgt gcgaatctac attcatcggc ccgcaaccgg
      481 caacaatgaa aaagcccctg agtcgtacga ctcaggggct tagtttggtg gaggcgaggg
      541 gagttgaacc cctgtccgaa gatcgtcggc acatcgcgtc tacatgtgta gccgattctt
      601 taagcttcgc agcgacccac gcctatcggc cggcttagaa tcgccgctag cccagtattg
      661 tctcgccccg cgccgctgag caccggcgtg ggaccagtcc gctgcatcgc gccattccac
      721 ccccgcggac ctcagatgga acgacgtctc agcctaaatt aggctgcgag tgccagttct
      781 tggttggcag ttgcgttttt cccggaattt tacgagttcc cgagatctcg acatgcgacg
      841 atggcgtcaa tatccccgtc gaaaccggtc gccccccttt cttggtgatc gtgaagcgtc
      901 agacgtaaaa cgtgagaggc cattcgctca tgaaaggatt cggctacttc tccgcttcct
      961 ttcgcgtttc acttttcacg tctcattacg tcttagatgc tcatcataca gcacaggtca
     1021 aggggatgcc aggagagaaa cggtacggcg ggaaaccagg gcagggaaac tcacggaacc
     1081 ggcgtgaagg tccaggtcca tccctgatcc tgttgcgtga agacctgtcc cttagacaac
     1141 tggcccgcga ggaactccgg aggaagtgtc accagcacct gttcctcgcg cacctgcccc
     1201 tgaaccaggc gtgtgccctt gcagccgcca cccccgacat attcctccat atccggcgtt
     1261 ctccgttcac ccaccagccc gaacatcggt cccatcacca gaatgctctt caactcaaaa
     1321 tgctcatagg cccctttcag aatcggtgcc gggcagaccg ccgagccatc acgatagtgc
     1381 tccgccttcg tcggaatatc cggcagcact tttggcgaca ccccatcctc cagcggccac
     1441 ttgaattcga actcgacctg cttcgtcacg tcggcgacgg ccgaaccacc gacgaactgc
     1501 ttgttccttt gaaagactcc caccactttg tacttcaacg gtgcgggctt cgaacgggag
     1561 gaccctttcg tgagccagac catggcttcg ctcacagtgg tcgacatctt cggccatgcg
     1621 cctgcctcac ggccgctcga catgagcacg gtccctgcga tcatcagcac caccagacgt
     1681 gtcacgcgtt cactcctctg caagctagcc cggactattc atgaatacct gctccgtcgt
     1741 ccgattctct cacccggcgt ggctgttctc attcggcctc cacgacggac tgccagtacg
     1801 cctgcgtcag cctcacttgc gagaagccgc ggcgggcttc ggtctcgaac gcctcgcgac
     1861 ggcattcatc aataatccgg gctagagaca ccccctagcg accttcccgc atcccgcgcc
     1921 gcggtacgcc gtcagccgcc tacaccggcg ttcacccgtc gctctacggg atactgccgt
     1981 tctcgccagg catcgagacc accttcgagc gggcgcacac ggtggatgcc tttcttcttc
     2041 aacagaaacg ccgtcctggc gctggagact tcattcgggc aggtgcaata gagcacgatg
     2101 tcgcgatcgc gcggtatctc ctggtgccga tgctcgatct cttccaacgt gaagttgatc
     2161 gcaccgggaa tggtgtccgg atcgagttgc agcgacaggg gatgccgcac atccaccacg
     2221 ctgatcgcct cgcccgcatc cagccgctgt ttgagctcgt ccacggagat cttcgacatg
     2281 cgcaggtgcc tgaggaactg ctgccgataa tagaacttat agccgatgaa tcccgtcagg
     2341 ccggtgagca gcaccccgaa caccagtccg cccgcttgat ccaacaaccc gacgagttgc
     2401 tccagttgat tgctgaataa cgtgccgact ccggcgctga ccgccgccca gatcaacgtg
     2461 cccgcgacat cgtacagggc gaaggtcgtc gcggccatgc cgacaatccc ggccagcggc
     2521 ggcgccaccg tattgaaacc ggggatgaac ttggccagca acagaccacg tggcccgtac
     2581 cggtgaaaca gattttccgt gtccctgacg caggagtccg gctcaagcga caggcgacag
     2641 agaaaaccca gcaccttgcc gcccttgatg cgaccaagat agtaccaggc gaggtccggc
     2701 gggagcgcag cggccaccgg cacgagcaac gccatggcaa ggctcatttt cccggccccg
     2761 acgagcgcac cagccgcaat gaggagagga atcgccggaa tcggaaatcc gatctgctca
     2821 acgaagatca cccagaacag caccgaggcg ccgtgatcgg cgagaaattg gaaggtgttc
     2881 atggcgatct ccttccgtgc gctcaggcgc tgcgataccg tgagaatcgc gcaaagagaa
     2941 tggcggcgaa ggggagggcc aggccgagca gggcctgcac aatcaacagg agtccgagct
     3001 gactatagtc gtcaggggtc tggatagcgt tggtcacggg atcgcgcacc tcccgggtca
     3061 ccacaaacag ctcgttgaga tacttcgtcc ccagttggcc gagcgacagc gcaaggttcg
     3121 tgaaggacgc catcacggca aaataggtcg ccttgagatt cgccggcgca gagttcgcga
     3181 tccaggccag catgggaatc atggaaatct gaccgagcgg cgattccaga gcggtattca
     3241 ctaacgcaat gaaccgcgca tcgaccagcc cgccggtcac ggccgccgtc cactcgtgaa
     3301 gcccgtaata catgctgacg atgggcaagc ccaggaagaa tgccgccagg gtgagaaagc
     3361 ccaccacata ggcaatggac cgctcagcca tgaatcgccg gaacagaaac atgcccgcga
     3421 gcgtgagagc gctgccgatg agcgacagca ccgacaggaa ctgttgatcg aacttcaact
     3481 gatcgatcat ccaccaggtc gccccttcgc cgggccctgg aacggcgcga aacacgaaaa
     3541 ccaccagcgc ggtacccacg agcacatgcc tcgcgtcggg agccagctcc ctggtgaggc
     3601 gcaccatcaa caacgagacg atggccatcg acccggcgaa cacgatttct tcactgtagg
     3661 caaagtctgc gactccgacc gtcagggtaa agagcacgaa ggccagactg ccacccagaa
     3721 tccaccaatt gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgacac
     3841 gcccgagcgt ctgcatcgtg gtgtgcatga gtttcagcct ggcttcatcg atgggcaccc
     3901 ctcgctcatc gactctgggc acggcctcga ccgtcatcgc gtccgccacc gtgtcctgaa
     3961 tcacatagcc gatcggcgac aacaacacgc tgagcacgta ccagacttcg gccggcatca
     4021 cggcgaccat cgcctcgcgg tgaccgatca ggccgaccat gatcaacaga ctggccgcaa
     4081 tcaccccggc gccaaaataa acaaggagac ttttccagcg ccacaacaga tcgaccagat
     4141 gtccgaacgg catcttgagc gcccagggaa tgcccgccca gaatcccaac gcggcaagaa
     4201 aggccgcgga caggcccaga taatctttga cgaagaaggt gccgacgatg ccggtcagac
     4261 cggaaatccc ggcggccata tagaccatga gcggcggcag ataggagagc tggaagtcgc
     4321 gcgcgagttc cagaatattg cggtcgatcc agcgggaggc agcagcaatc acgaatgcgt
     4381 atccagggac ggcggtgaag ggatcgttgt ctctctctgt tacccgtacg gggccaccat
     4441 ttgcaagcag cccggagagt cggatggaac ggccggagcc tcacgacccc ggcccttcca
     4501 atcagtacac gtacggctta cttcggaaac tctacgggcg cagccacgtg ctgccaccct
     4561 tcgccgttga gcgaccgtaa gaatgcgacc agatcctgct gctcgggccc ggtcagttcg
     4621 agcgggatca cggtgttatc gaggtgcgga ttcttgatgc caccctggtt gtagaaccgg
     4681 acgacatctt cgagcgtcgc aaaccggccg tcgtgcatgt acggcgccgt acgcgcgatc
     4741 tctcgcaacg tgggcgtctt gaacgcgccg atatcctcgg cattcttcgt caccatgtag
     4801 cgccccaaat cgacggtgtt cgtatcccag ccgataccga ggttatgaaa tttctcgtcg
     4861 gagaagttga acccggagtg gcatcgtgtg cagcgggcct tccctcgaaa cagttcgagc
     4921 cctcgcttcg ccgaatcgct caaggcctgc tcatcgccgc ccatatcgaa ccggtccacc
     4981 gggctgttgc ccgacaacag ggtccgctga aaagcagtga tcgcgcggcc cacatcgccg
     5041 acggtcacct cgcggccgaa cacctcccga aagagcatcc gatagtcctc gatgctcttg
     5101 atcttgctca ccaactcatt gtggtcgaga aacccgtgct caaccgggct gacgaacgga
     5161 ccgaccgatt gatcctccaa cgtgtcggct cgaccgtccc agaattgcgc cttgctatac
     5221 acccggttga tcgccgtcgg ggcgctgcgt ccgccctgta accgataaat acccgtcgag
     5281 accggttgcc catcggtgaa tgccaggccc ggcatgtgac aggttgcgca agccactgtc
     5341 ccgttcttcg agagtcgctt gtcgaaaaag agccagcgcc ccagctcgat cttccgcgcc
     5401 gtctgcggat tgtcggccgg aatatatccg ctgggatcct ccaatcccag cggcacctcc
     5461 tgtgccacgg gaacggcgcc tggttgcgcc accgacggct cacccatgac cctggtggcc
     5521 gccaagccca tcgccccgac ggtcgccacc accactgtac cgaccatcca cttgatgctc
     5581 atcttgccca tgatctcctc ccttgctcct tcttccttcg atgctccttc cgtatggatc
     5641 atcgaaaccg cagagtcgtg ccaggccgat cctggcgctc catgtgccgt tcgacctttt
     5701 gaaggaattc ttcttccgac aggggcgttt gctgcccttc cgagaaggcc accgtctgat
     5761 gacccgttgg cctcgacggc gtcgcgcacg cctgcaacat gatcacggcg aaccccatac
     5821 cgacggctat cacaatctgt cgcatacccc acctccttgt tccatggctg cattgttgcg
     5881 aggtcataca gagacgatac cggttgcctg agaatcagtc caattgatag tctatggcat
     5941 cacgataaac tgagcttatc aatagaggag ggcattcatg accctgacgg aactgcagta
     6001 catcgtggcg gtttcgcgcg agcgtcattt cggacgggcg gcggatcggg tcttcgtcac
     6061 ccaaccggcc ctcagcctgg ccattaaaaa actggaagat gaattggggg tggcgatttt
     6121 cgagcgacgg aagaatcacg tcgagctcac gccgctcggg gagcagatcg tccatcaggc
     6181 tcaacgcgtg ctggaggagg cggatcagat caaactgctc gccgcgcagg ggaagaacca
     6241 actcgtcggc gccttgcggt tcggggtgat tgcgacggtg gggccgtatc tcttgcccga
     6301 cctgatcccg ctgctgcaca agcgcgcccc cggcatgccg ctcgaaatcg aagaaaatct
     6361 gaccgtcaat ctgaccgcca tgctgaagag cggcaaactg gacgtgatta tgatcgcgct
     6421 accgttcgaa gaaccgggca ttctcaccca agccctctat gacgaaccgt tcaaggccgt
     6481 cgtgcccgcc gatcaccggc tggccaaacg gtcgaaaatc gattcggcac tcttgagcac
     6541 ggagcgcgtg ctcttaccgc acgcaggaca ttgtttccgg caacaggtcc tggagagctg
     6601 cccggaactc agccggtcgg acacggaaag tttgcagggc aattcgctgg agacgatccg
     6661 tcagatggtg gcctccggac tgggcatcac cgtcatgccc tgcagtgccg tcacggccaa
     6721 acatcagaac aaacggctgg ctgtcctgga gttcatgaag ccggtgccgg aacgcagaat
     6781 tgcgttggca tggaggaagg gctttacaag accggcggtg attcctgtga ttcaggaggc
     6841 ggtcggtgca ttgaaaattc ccggactgat ggccattgcc tcaggacaat aattgtccca
     6901 cgatgaaagg aagaagaatg gccgagtcac gaatcagcac gcatgtgctg gacattgcgc
     6961 aaggccgacc tgcacagggc attcccgtca cgttggaagg ccaggggttg gcgggaatct
     7021 ggaaactact cggcaagagc agaacggatg cggacggccg gagcaacgat ctctatccgg
     7081 caaagcagcg acttcagccc ggagtctacc gtctcacatt tgacgtctcg acctattttc
     7141 gctcacagca cgtgacgagc ttttaccctg aggtggtcgt catcttcact ctgtccgacg
     7201 cgggacagcc gtaccacatt ccgctgctcc tgagcccctt cggctacagc acctatcgag
     7261 ggagctgaga gaagatcgac ggtccactct gccggttatt cccgcgcccc cgccgacaag
     7321 agaatgtcgg cgatcactct gtagcctttc tgcctggcat gccggagcgg cgtcaccccg
     7381 tctccgtcgc caaggttgag atcggctcct gcagccacaa gcaatccgac gatggtctga
     7441 tgtgtcggcc ctccgtcacc gagaatgatg gcttccaaca aagccgtcca gcccagccgg
     7501 ttcacgtggt tcacgtcgat cttggtcttc agcagttctt tcacgacttc cacgtggcct
     7561 cgttcgcacg ccgggatcaa cgcggtgcct ccgtagcgat tgtagatcgt aaagtccgga
     7621 tcggcagcca acgtcattcg cagaatgtcg agatacccgc tggccccggc caacagcaga
     7681 ggactgtttt gcatggcatc ctgcgtgttc acatcggagc cggtcaggat cagtagcttc
     7741 gcgatctcga catggttccg ataagttgca gccagcaagg cggtgcgccc ctgcggatca
     7801 cgggcccgga cgctggctcc ttctttcaac agctgtcgca cagcctcgac atcgttgcgt
     7861 tctgccgccg caatcagagc ctggtctccc gtccgatgac ccggctcggc gccggccagg
     7921 ctgacgctcg ccatgaagag cagaccggtc catgacagag cgcacatcca acccatctga
     7981 ttccgcctca ttgtcgtcgc tcgaccagct cgcgcaccgc cgcccgcatt tcggattgtg
     8041 gcagccagac gcggccgcca tgaccggcca acacccattc gaatgaacag ttcgtcagtt
     8101 gctcgattga gcggtgcaag gcctctgcat tccacaccaa ctgctgcggg gaggccagct
     8161 ccctagcctc cgggtcccac caaagatgat cgccggtaaa cagaaaccgg tcccgatgca
     8221 gcagacagag gctgcccgcc gtatggccgg gaacggggat gatgcggaag tcttcgctgt
     8281 acgagcgagg ttccggtcct tcaatgatca cctccgcctc gggcatcgca tggacatccg
     8341 cacggtggat gatccgccgg gctccgaacc ggcgcgcata cctggcggca tccgccacat
     8401 cgtcttcatg ggtcaaaaag atctgtgcga ggcctcccat ccgatcgaag acatcgacaa
     8461 gcggtttgag ataccgcggc gaatcgatca gccagttgcc gtccggatgc tgcacgaaga
     8521 aactgttcgc cccgaacgac ttttccgaat tgaaaccgca gtagtagacg ttcccttcaa
     8581 tcgggagagg gaagtcggcc ttcgcggcac tcaagagggt ttggttcgta tgctcggccc
     8641 cgatcgaccc ggtcgggcag gcgagcaacg cgcgataggc ctgccttctc tccggttcgg
     8701 tggccggctg atgagtcacc gcagaatact ctcccacttc ttcgaagctc gacggagcca
     8761 attgtcggca ggcatcgcaa ttgatgcagg tggaatcgac atagaagtt
//
LOCUS       DAOQRM010000100         8735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_7514_length_8735_cov_12.217972, whole genome shotgun sequence.
ACCESSION   DAOQRM010000100 DAOQRM010000000
VERSION     DAOQRM010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_8735_cov_12.217972"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKK81_08865"
     CDS             complement(<1..517)
                     /locus_tag="PKK81_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNV33028.1"
                     /translation="MQRQSEKQSVVWSTVMGFVLLGGVIPVAAAEDTKKPAEQAAPAA
                     KKTPEKKATAKSGSQKAKPAATAKDQAPKYTSAETAAKAQACFGEAPKIQSVTPDEAK
                     AGEKVTISGRGFGPADCLRSLSFGPGYPAAFTFVNDTQITATVPTGRRTGMAMMTVTT
                     ASGEDSKAFLVK"
     gene            complement(573..2471)
                     /gene="acs"
                     /locus_tag="PKK81_08870"
     CDS             complement(573..2471)
                     /gene="acs"
                     /locus_tag="PKK81_08870"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNV33029.1"
                     /translation="MALMDEKIDTLLKEGRVIQPTAKTKAAAHIQDYEQAYQASIADP
                     EAFWGGVAKELDWFSPWSKVLDWNYPWAKWFVGATCNIAYNCLDRHANTWRRNKVAII
                     WVGENGEERIFTYGELLRQVNRCANALKALGLQKGDRVTIYLPKIPEQVVAMLACARI
                     GVIHSVVYSGFSAPALASRIQDAEARVVITADVGYDRGKTINLKGVVDDAVRTCPSVE
                     KVVVVRRESPGVALEAPKEIDWVEWLQAQSAVCQAEALDAETPLYILYTSGTTGKPKG
                     VVHVHGGYMVGTYITTKYVFDLKEEDVYFCVADPGWVTGHSYIVYGPLLNGATILMAE
                     GKPDYPNPGRWWDLIARYGVSIFYTTPTAVRLLMKYGEDWPKKYDLSTLRILGSVGEP
                     INPEAWEWFYRVTGSDKPIMDTWWQTETGSILVTPLPSVPLKPGSATRPFLGIEADVV
                     DKQGNSLPNNAGGFAVIKKPWPSMMRTIYKDPDRYKVYWNTIPNCYTAGDVCRKDNDG
                     YMWFMGRADDVIKVAGNRIGTAEVESALVSHEAVAEAAVIGKPHRTAGEAIKAFVILK
                     QDYQDSKELVQSLKDHVLKELGKIAVPQEIDIVPSLPKTRSGKIMRRVLKAKELGQDV
                     GDISTIED"
     gene            2690..>3841
                     /locus_tag="PKK81_08875"
     CDS             2690..>3841
                     /locus_tag="PKK81_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNV33030.1"
                     /translation="MRSSTFPHLRRMFVCLVAMCAAGTVGILSIAHAQVGKPEGLYYK
                     SWAVVVGVENYLVAPKVPGALESAHAVAAALRGLGFDEVIELQDKEASSKHLLQILND
                     YLPRKVGRQDRVLFFFAGHAGVTQDSQSKELGYLVPWDAQLNNPAKAITFDQLKEFSR
                     RSASKHILMLFDANIRGWEVTAPQPLSLEGRLSPEDDTEKRAVQVLTAAEKEEAVGRA
                     PGQSAFVTALVAGLNGAADLNKNGWVMASELAAQVKQDVEAATKGAQHPQFVQLEGDG
                     DVILIEGRKGAFQAGKEPTSEADRMREARIQYEQAFALLQQQKSAEEALERLNRAIGY
                     DPSFGDAYVLKSYIRLEVLPNLEESLLAARAAVQHAPQNPDSHYSLALAL"
     assembly_gap    3842..3941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4445..5011
                     /locus_tag="PKK81_08880"
     CDS             4445..5011
                     /locus_tag="PKK81_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33031.1"
                     /translation="MRRVEGAFSKFTGSALALVLLIGLTACGGPPNWVKKGSGAFNEK
                     SDKSFYGVGSVVGVRNEPLAWDTAENRSRAEIAKTFETYTAYLMRDYAASTTAGDFSR
                     NSEEQNIERAVKTFSAVTLNGVKPMDRYKDEKSGTYYVLTKLSLEDMKNNLEQAKELN
                     GQVRDFVRKNADRLFDRLEKEEDKRATK"
     gene            5050..5679
                     /locus_tag="PKK81_08885"
     CDS             5050..5679
                     /locus_tag="PKK81_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNV33032.1"
                     /translation="MIEWRNRSVGRTFATAALVAVAAAASGCGHETKVTRVDTGIVTD
                     LSGRWNDTDSQMVAEAMVREALGNPWLGNFTKGKSRQPVVIVGTVLNKSHEHINVQTF
                     ISDLERELTNSQKVTFVAGKGEREEVREERREQAVHAREDTQKAPGKEIGADYMMRGS
                     ISTILDEQDGAKAVFYQIDLELVDMENNVKAWFGQKKIKKVVEKKRTIF"
     assembly_gap    5870..5969
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5970..7025
                     /locus_tag="PKK81_08890"
     CDS             <5970..7025
                     /locus_tag="PKK81_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33033.1"
                     /translation="YAAMGQLMEAVVEARQIDHRLNVLSDSAKEKDGYREDAFARYLT
                     GVLYEATGDLNNAFIAYRKAYEIYESTRGWARTPMPPMLRADLLRTTDALHMTAEFEE
                     YRRQFPDTGWVSLQGQPDLAQVVVIGYNGRAPRKEEVHLDIPVSLGALQLVLLNRGVI
                     HASNRQERRVADSVLYGLNGRVVRVALPRLVPQKTQVSHESITLVPRNGEPITGQSQL
                     MYNSTALAEKSLSDRMPGITTKALARAAVKFTAAEAATRGSQHAVNKGDAAWVGLLVG
                     LLTHGLAVASEVADTRSWRTLPDEIQISRLWVPAGEYESRIQPVLRSGGATQAGVSRP
                     LVLRAGHTVFLIERVVL"
     gene            7022..8143
                     /locus_tag="PKK81_08895"
     CDS             7022..8143
                     /locus_tag="PKK81_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNV33034.1"
                     /translation="MKLVTGRGRAAVLSALLLLLAGCGWFGGTARPAWIDGGSPQFPS
                     AQYLVGVGQADSRPQATEQAYAAVSRIFKAEITAQAKDWESYLVVESRGQTSTERRLT
                     LDNVTRVTTDKVLENVQVLDTWFDQKTRQYYALAGMNRAQAEAAMVERLNELDRTIQT
                     EVTEAHQTQDKLSRVRNLKRAAKNLVLREAYNTDLRVLRSNGQGNASTYRVAELTAEL
                     EQFLAKNLVMAVDLSGDQAEPLERALVDGLAQEGFTVVGRGAGAVPAELLITGSVRLW
                     PIEVNDPHFRYVRWCAESVIEEVATHRVVGALSKGGKEGHVTEREAAAKAVRVMQQEF
                     ASDLARSVAAHVYGEMDLPASASTPSGCPKEAVQPPVSR"
     gene            8178..8426
                     /locus_tag="PKK81_08900"
     CDS             8178..8426
                     /locus_tag="PKK81_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33035.1"
                     /translation="MSKIGTRKSGEQGNGGRRLSSQAMKKRLRERLTADTQQVLKSDI
                     VSIFRKQGYYEELPLDELQDRLSKLQSPLADSLLKGRV"
     gene            8428..>8735
                     /locus_tag="PKK81_08905"
     CDS             8428..>8735
                     /locus_tag="PKK81_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV33036.1"
                     /translation="MPYYYFDSTALVKRYSMERGTRVVNKLMVKRGKVAILPMWSVTD
                     FYSALSVRAQQGEITRDDCYSVLYKFEMEASQGLFQFISPTMETYLAAKELILDYPAL
                     R"
BASE COUNT         1688 a         2397 c         2692 g         1758 t
ORIGIN      
        1 acttcaccag aaatgccttg gaatcttcac cggacgcagt cgtgacggtc atcatagcca
       61 tgcctgtgcg tctgccggtc ggtacggttg ccgtgatctg ggtgtcgttg acgaatgtaa
      121 atgcggcggg atacccggga ccgaaggata gtgagcgcag acagtcggcg ggaccgaatc
      181 ctctgcccga gatcgtgacc ttctcgcctg ctttggcttc atccggcgtg accgactgga
      241 tcttcggtgc ttctccgaag caggcttgcg ccttggctgc cgtttctgcc gaagtgtact
      301 tgggggcctg gtctttggcg gtggccgccg gcttcgcctt ctgacttccg ctcttcgcgg
      361 tggccttttt ctcgggagtc ttctttgctg ctggtgcggc ctgttcagcc ggcttcttgg
      421 tgtcctcggc ggccgcgacg gggatgaccc ctcccagcag cacaaatccc atcaccgtcg
      481 accacaccac actctgcttc tcgctctgcc tctgcatgac cggcctccat gcacacaata
      541 aaagatgatg cattgactga agacgaatcg tctcagtctt cgatcgtcga aatatcgccc
      601 acgtcctgtc ccagttcctt ggctttgagg acccgccgca tgatcttgcc ggacctggtc
      661 ttcggcaacg acggcacgat gtcgatttct tgcggcaccg cgattttccc caactccttc
      721 agcacatggt ctttgagcga ttgcaccaac tctttcgagt cctggtagtc ttgcttcagg
      781 atgacgaagg ccttgatcgc ttcaccggcg gtgcgatgcg gcttgccgat gaccgccgct
      841 tcggccacgg cttcgtgact gaccagggcg ctttcgactt ccgccgtgcc gatgcggttg
      901 ccggcgactt tgatcacatc gtctgcgcga cccatgaacc acatgtaccc gtcgttgtcc
      961 ttccggcaga catcgcccgc cgtataacag ttggggatcg tgttccagta gaccttgtag
     1021 cgatcgggat ctttgtagat cgtgcgcatc atggagggcc agggcttctt gatcacggca
     1081 aagccgccgg cattgttcgg gaggctgttc ccttgcttgt ccaccacgtc ggcctcgatg
     1141 ccgagaaacg gtctggtggc cgatcctggc ttcagcggaa cggacggtag gggcgtgacc
     1201 aggatggagc cggtttcggt ctgccaccag gtatccatga tcggcttgtc gctgccggtc
     1261 acgcggtaaa accattccca ggcttccggg ttaatgggtt cgccgacgct gccgaggatg
     1321 cgcaacgttg agaggtcgta cttcttgggc cagtcctccc cgtacttcat caggagccgg
     1381 accgccgtcg gcgtggtgta gaaaatcgag acgccgtagc gggcgatgag atcccaccag
     1441 cggccggggt tcggatagtc gggtttgcct tcggccatga ggatcgtcgc gccgttcagc
     1501 agcgggccgt agacgatgta actgtggccg gtgacccagc ccgggtcggc gacgcagaag
     1561 tagacgtctt cttccttgag gtcgaacaca tatttggtcg tgatgtaggt accgaccata
     1621 tagccgccgt gcacatgcac gacgcctttg ggtttaccgg tcgtgcctga ggtgtagaga
     1681 atgtagaggg gagtttccgc atcgagcgct tctgcctggc agacggcgct ctgtgcttgc
     1741 agccactcga cccaatcgat ttctttggga gcctcgaggg cgacgcctgg agattcgcgt
     1801 cgcacgacca ccactttttc gacggacggg caggttcgca cggcatcgtc gacgacgccc
     1861 ttgaggttga tggtttttcc tcgatcgtac ccgacatccg ccgtgatgac gacgcgcgct
     1921 tcggcgtcct ggatgcggct tgcgagggcc ggggcgctga agccggaata gaccacgctg
     1981 tggatcacgc cgatacgggc gcaggccagc atggcgacga cctgttcagg aattttcggg
     2041 agatagatgg tgacgcgatc gcccttttgc aggccaagtg ccttgagggc attggcgcac
     2101 cggttgacct gacgcaacag ttctccatag gtgaagatgc gttcctcgcc gttttcaccg
     2161 acccagatga tggcgacttt gttgcgccgc caggtgttcg cgtggcgatc caggcagtta
     2221 taagcgatat tgcaggtcgc gccgacgaac catttggccc aggggtagtt ccaatcgagc
     2281 accttgctcc acggcgaaaa ccaatccagt tccttggcca caccgcccca gaacgcttcc
     2341 ggatcggcga tggaagcctg gtaagcctgc tcatagtcct gaatatgagc agccgccttg
     2401 gtcttggcgg tcggctgaat aacacgtcct tctttcagta gggtatcgat cttttcgtcc
     2461 atcagtgcca tgcctccaca aagcgcgggc ctgtccggca cgcgattcaa tcatcaccgg
     2521 cattatgcgt atggggcgaa ggaactgcaa ccctcagcgc ccctgtgaga ccttgcctga
     2581 tgcagacgga ggccgcgtgg cgcaaggccg gagcactccg cctttcttga cagtcaggag
     2641 gagacgaggg tacgctgatt gcgttgcact tcactggatt aaccccttca tgcgctcgtc
     2701 tacattcccc catcttcgtc ggatgttcgt atgcctggtg gccatgtgcg ctgctggaac
     2761 ggttggaatt ctctccattg cccacgcgca ggttggaaag ccggaaggac tctattacaa
     2821 gtcctgggcg gtcgtggtcg gtgtcgagaa ctatctcgtg gcaccgaaag tgcccggcgc
     2881 gttggagagt gcgcatgcgg tggcggcggc gttgcggggg ctcgggttcg atgaagtgat
     2941 cgagctgcag gataaagagg caagttccaa acatctgctc cagattctca atgactattt
     3001 gccccgcaaa gtagggcgtc aggatcgcgt gctgttcttt tttgccgggc atgcaggggt
     3061 cactcaggat tctcaatcca aagaactggg ctatctggtt ccctgggatg cgcagctgaa
     3121 caatcccgct aaagccatca cgttcgatca actcaaagaa ttcagccgac ggtccgcttc
     3181 caaacacatc ttgatgttgt tcgacgcgaa catccggggg tgggaggtga ccgcgccgca
     3241 gccgctttca ctcgaaggcc gtttgtcgcc cgaagatgat acggaaaaac gagccgtcca
     3301 ggtcctcacg gcggctgaga aagaggaggc ggtcggacgt gctccgggcc agagcgcctt
     3361 tgtaacggca ttggtcgctg ggctgaacgg ggctgccgat ctcaacaaga acggctgggt
     3421 catggccagc gaactcgcgg cgcaggtgaa gcaggacgtg gaggccgcga cgaagggggc
     3481 tcagcacccg cagttcgtgc agttggaggg cgacggggac gtcattctga ttgaaggacg
     3541 aaaaggcgcc ttccaggccg ggaaggaacc gacgagtgag gcggaccgga tgagggaggc
     3601 ccgcattcag tatgaacagg cctttgcatt gctgcagcag caaaagtcgg ccgaagaggc
     3661 gttggaacgg ctcaaccgcg cgatcggcta cgatccgtcc ttcggcgatg cctacgtatt
     3721 aaagagttac atccgtttgg aagtgctgcc gaatctggag gagtcgcttc tcgccgcccg
     3781 cgcggcggtg caacatgcgc cgcagaatcc cgattcccac tactccctgg cactggccct
     3841 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntattccgcc aacacggcgg
     3961 cggccttggg atcctggata tcgagttgaa ccactttaat aatgccgtct tcaatctgca
     4021 cgtcagccat gtgtctgtct cctccgtaaa acctgtgtct aggagtctgt ccgagtaatg
     4081 ccgttctgct gcgacgaatg atctgccggc atctgcctcg ttctcggccc gtaaaaatcc
     4141 tcaacgtatt cagcgaatac gcttccggtt ttttcgagcc tgcggcctcg catccgccgg
     4201 caactccttc gtctcgctac gaaggattac tcggacaggc tcctagggta gatgaatcgg
     4261 ccctgtggtc aaccggaaca gtcgttgcgc gttgacagtg ttaggggtgc atgttagcat
     4321 aaaatctgct tttttcgccc aactgtgtcg ggtgacagtg agccacacac gtctctctcc
     4381 catcatcttg ccacacacca cgaagccgtc agcgttcagc tgtgtcacac caaggaggaa
     4441 tcgtatgcgc agagtagagg gggctttttc caagttcacc ggcagcgcgt tggcgctcgt
     4501 actcctcatc gggctgactg cctgcggcgg tcctccgaac tgggtcaaga agggatccgg
     4561 cgccttcaac gaaaagagcg ataagtcttt ttatggtgtc gggtcggtgg tgggcgtgcg
     4621 gaacgagccc ttagcctggg acacggctga gaaccgcagc cgcgcggaaa tcgcgaaaac
     4681 cttcgaaacc tacacggcct atctcatgcg cgactatgcg gcctcgacga ccgccggtga
     4741 cttttcccgc aacagtgaag aacagaacat cgaacgggca gtgaagacct tctcagcagt
     4801 cactctgaac ggtgtgaagc cgatggatcg gtacaaagat gagaagtccg gcacttacta
     4861 cgtcctcacc aagctgagcc tcgaagacat gaagaacaat ctggagcagg cgaaggagtt
     4921 gaacggccag gtccgcgact ttgtgcgaaa gaatgccgac cggttgtttg atcgtctgga
     4981 gaaggaagaa gacaagcggg cgacgaagta agcggacgtg ccggtgcgcg cagaccagga
     5041 ggagagagca tgatcgagtg gcggaatcgt tcagtgggcc ggaccttcgc gacggcagcg
     5101 ctggtggctg tggccgcggc ggcgagcgga tgtgggcacg aaacgaaagt gacgcgcgtc
     5161 gataccggca tcgtcacgga cctcagcggg cgctggaacg acaccgattc acaaatggtg
     5221 gctgaagcca tggtgcgaga agccctgggc aatccctggc tcggcaattt taccaagggg
     5281 aagagtcgcc agccggtcgt catcgtcgga accgtcctca acaagagcca tgaacacatc
     5341 aacgtgcaga ccttcatcag tgatctggag cgggaactga cgaactcgca aaaagtgacg
     5401 ttcgtcgccg gcaagggcga gcgggaagag gtgcgcgagg agcggcgcga gcaggccgta
     5461 cacgcccgtg aagacacgca gaaagctccc gggaaagaaa tcggcgcgga ctacatgatg
     5521 cgcgggagca tctccacgat tctggatgaa caggatgggg ccaaagcggt cttctatcag
     5581 atcgacctgg aactggtgga tatggagaac aacgtgaagg cctggttcgg gcagaagaaa
     5641 atcaagaagg tcgtcgagaa gaaacgaacg attttttagc ccgacgtatt ctgttgagac
     5701 gccactcccc atcgaccacg gcctcctttc agggaggccg tttcatttct ctcccctgga
     5761 tgcgcgttgt cttcctgggc ctgttgtgga ccctgtccgg gtgcgggttc tccgccaatc
     5821 attatctcct catcgaccag agtttgctgg cgaacgaccc caggcgggcn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt atgcggcgat ggggcagttg atggaggccg
     6001 tcgttgaggc gcggcagatc gatcatcgat tgaacgtgct gtcggacagc gccaaagaaa
     6061 aagacgggta tcgggaggat gcgtttgccc gctacctgac cggtgtgttg tacgaggcga
     6121 cgggcgatct caacaatgcc ttcattgcct accgtaaagc ctatgagatt tatgagtcca
     6181 cgcgggggtg ggccaggacg cccatgccgc cgatgcttcg cgcagatctg ctccgaacca
     6241 ccgatgccct gcacatgacg gctgagttcg aggagtatcg tcggcagttt cccgacacag
     6301 gatgggtgtc gctgcagggg caacccgatc tcgctcaggt ggtggtgatc ggctataatg
     6361 gccgcgcgcc gcgcaaggaa gaggtccatc tcgatattcc ggtcagcctg ggcgcactgc
     6421 aactggtcct gctgaatcga ggcgtgattc acgcgtccaa tcgtcaggag cgtcgagtgg
     6481 cggacagtgt gttgtatggc ctgaacgggc gtgtcgtgcg ggtggccctg ccccggttgg
     6541 tgccacaaaa aacgcaggtc tcgcacgaat ccatcacgct ggtgccgcga aacggagagc
     6601 cgatcaccgg acaatcgcag ctgatgtata acagtacggc gttggcggaa aaatccctgt
     6661 cggaccgcat gcccggaatc actacgaaag cccttgcgcg cgcggccgtg aaattcaccg
     6721 cagccgaagc ggcgacccgt ggatcccaac atgccgtgaa caagggggat gccgcgtggg
     6781 tggggctctt ggtggggttg ctcacgcatg gtctggcggt ggcgtccgaa gtcgccgata
     6841 cgcgaagttg gcggaccctg ccggacgaaa ttcagatttc ccgcctgtgg gtcccggccg
     6901 gcgaatacga aagtcggatt caaccggtgc ttcgaagcgg cggggctacc caggccggtg
     6961 tgtctcgtcc gctggtcctt cgcgccgggc acaccgtctt cctgatcgag cgggtggtgt
     7021 tatgaagctg gtcacggggc gagggcgagc ggccgttctc tcggcattgc tgctgttgct
     7081 tgccggctgc ggatggttcg gcggaactgc ccgtcctgcg tggatcgacg gaggaagccc
     7141 ccagtttccg tcagcccagt atctcgtcgg ggtgggtcag gccgattcgc gaccgcaggc
     7201 aacggagcag gcctatgctg ccgtctccag aatttttaag gccgagatca cggcgcaggc
     7261 gaaagactgg gaatcgtacc tggttgtgga gtcccgcggg cagacgagca cggagcgtcg
     7321 cctcacgttg gacaacgtga cccgtgtgac gaccgacaag gtgctggaaa atgtgcaggt
     7381 cctggatacc tggtttgacc aaaagacgcg acagtactat gctctggcag gcatgaaccg
     7441 ggcgcaagcc gaagcggcga tggtggagcg cctcaatgaa ctcgatcgta cgattcagac
     7501 cgaagtaacc gaagcgcatc agacccagga caagctctcg cgcgtgcgta atctcaaacg
     7561 ggccgccaag aacctggtgc ttcgcgaggc ctataacacc gatcttcgcg tcttgcgctc
     7621 gaacggtcag ggcaatgcgt cgacgtatcg tgtggcggag ttgactgctg aattggaaca
     7681 gtttcttgcc aagaatctcg tcatggccgt cgatctgtct ggcgatcaag ccgagccgct
     7741 tgagcgtgcg ctcgtagacg gcctcgcaca ggaaggcttc acggtggtgg ggcgcggcgc
     7801 cggtgccgtt cctgcagaat tactcattac cgggtcggtg cgtctctggc cgatcgaagt
     7861 gaacgatccc cacttccgct atgtccgctg gtgcgcagag tccgtgatcg aagaggtcgc
     7921 gacccatcgt gtggtcgggg ccctgtcgaa aggcgggaag gaagggcatg tcacggagcg
     7981 cgaggcagcc gctaaggcgg tccgtgtcat gcagcaggag tttgcctccg atctggcccg
     8041 atccgtggcc gcgcatgtgt atggagagat ggatctgccg gcgtcggcct cgacgccgtc
     8101 cggttgtccg aaagaggcgg tgcagccgcc ggtcagtcgt taacgcggtg agtcaaccac
     8161 aggagagagg agcagggatg agtaaaatag ggacccggaa gtcgggtgag caaggcaacg
     8221 gtggacgccg gttgtccagt caggcgatga agaagcggtt gcgcgaacgg ctgacggcag
     8281 atacgcagca ggtactgaag agcgacatcg tgagcatttt tcgcaaacag ggctactacg
     8341 aggaattgcc attggatgag ttgcaggatc gcctctccaa gctccagtcg ccgttggccg
     8401 acagtctctt gaaggggagg gtgtaggatg ccgtattact atttcgactc cacggcgttg
     8461 gtgaagcggt acagcatgga acgcggaacg cgggtggtga acaagctcat ggtgaaacgg
     8521 ggcaaggtcg ccattctgcc tatgtggagc gtcacggatt tttattcggc cctctccgtt
     8581 cgcgcgcagc agggtgaaat caccagggac gactgttatt cggtgctgta caagttcgag
     8641 atggaagctt cgcaagggct cttccagttc atctccccga cgatggagac ctatctggct
     8701 gcgaaggagt tgattctgga ctatccggct ctgcg
//
LOCUS       DAOQRM010000101         8673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_7593_length_8673_cov_19.926317, whole genome shotgun sequence.
ACCESSION   DAOQRM010000101 DAOQRM010000000
VERSION     DAOQRM010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7593_length_8673_cov_19.926317"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /gene="bamA"
                     /locus_tag="PKK81_08910"
     CDS             complement(<1..1639)
                     /gene="bamA"
                     /locus_tag="PKK81_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNV33037.1"
                     /translation="MTKSGGRRWVPILILALVALYAFGVGAPLYAQDGVPTVTSVTIR
                     GQKRIELQAIEGRLTLKANDRFTADALREQVKILYGTGYFEDVQVETEPAAGGVSVGF
                     VVREKPFITEIVFDGNEELSDDKLKEKVTIKSQTFLDQQQAKESAEKIRLAYQEDGYY
                     NCQVIPVIQAVDEDRKRLTYFIKEGTKAKVLHINFEGMRAVTKEEIFKVTATREWIPW
                     YGLITQLKVPSLLSDAGVLKREELGNDIERMREVYLNKGYLNVQISQPTLELSEDKKW
                     FEINYSIVEGEPFIVQEVGFRGNTVFEDHELREGLNIRPGEIFQRAKIRGEITRITDL
                     YGAKGYVFADVVPNVTPDNNSRTATILLNVKEGEMMRIREIHVNGNDKTRDNVVRREL
                     RLDEQDVVDTLALKRSFQRLNNLNFFETVEILPQQVDVDKVDLNVKVKEKPTGQFSIG
                     GGFSTLDKLVAIADITEGNLGGNGWLGRIRGQLGQQRSLGLVTFRNPYVNDSYNALQL
                     DIYRSMTNYISYFESKSGVSATWSRWLSEYVNGSISLF"
     gene            1956..2897
                     /gene="galU"
                     /locus_tag="PKK81_08915"
     CDS             1956..2897
                     /gene="galU"
                     /locus_tag="PKK81_08915"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNV33038.1"
                     /translation="MTRTDVRKAIIPAAGLGTRFLPATKASPKEMLPLVDKPLIQYVV
                     EEAVASGIEDIIVITGRGKRAIEDHFDRSLELEENLKGSGKGQVLNQMRHISNLANFC
                     YVRQPEAMGLGHAVLCAQHLIGDEPFAVILGDEIIDAAVPGLAQLIHIYKQRKGAVLG
                     VQEVPPQEVSRYGIISARRLGKGLHRVDDLVEKPAPADAPSNLAVIGRYVLPPEIFPI
                     LRKTPPGKNGEIQLTDALRQLVKHTPMFAHEIEGQRHDAGDKLGFLIATVELALKNPS
                     LGPAFGEFLQQRLRPTAADVRRRGPSRSGQGRTRTTN"
     gene            2984..5479
                     /gene="lon"
                     /locus_tag="PKK81_08920"
     CDS             2984..5479
                     /gene="lon"
                     /locus_tag="PKK81_08920"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNV33039.1"
                     /translation="MAEAVEQDFSSNQNLEPPDQLPLLPVRDIVVFPYMVLPLFVGRE
                     MSIKAIEAALAGNRMLFLATQKSLDVENPQPDDIHAVGTVGIIMRMLKLPDERIKILV
                     QGLAKGRIEEYIQNDPYYSVRIEKLVETKQAGSTLETEAVMRTVKEQIEKIVSLGKVL
                     IPDVMVVIENLEDPGRLADMVASNLGLKVDITQAVLEIVDPIQRLRQISEILSKEIDV
                     LSMQQKIQAQAKGEMDKTQREYFLREQLKAIQKELGELDERAEEVAEFRKRIKDAKMP
                     EKVLKETEKQLKRLEKMHPDTAESATVRTYLEWMVELPWNKKSKDNLDLKAAMKVLNE
                     DHYDLEKVKERIVEYLAVRKLKEKMKGPILCFVGPPGVGKTSLGKSIARALGREFVRI
                     SLGGVRDEAEIRGHRRTYVGALPGRIIQGMKQAGTNNPVFMLDEVDKVGMDFRGDPSA
                     ALLEVLDPEQNSTFTDHYLGVPFDLTEVMFVTTANLMDPILPALRDRMEVIDIPGYTE
                     EEKLGIAQKYLIPRQMNEHGITEKHIRVNEATIRHVISHYTREAGVRNLEREIANLMR
                     KVAKKVAEGKSECHTIDQTNLNKFLGVAKFVPEAELEKDEIGVATGLAWTESGGDVLY
                     IEATVMKGKGQLTLTGHLGDVMKESAQAALSYVRSREKTLGISPDIFAKNDIHIHVPA
                     GATPKDGPSAGITMATAIASALAQIPVRRDLAMTGEITLRGRVLPIGGLKEKILAAKR
                     AKLATVVLPKRNKKDLEEIPKHILKGIELIFVDTVDDVIKAALRRSSSRKPRASAPKT
                     PVKKSARATTSKRTTGSPRKSLRLPTASPSLIL"
     gene            5481..6530
                     /gene="thiL"
                     /locus_tag="PKK81_08925"
     CDS             5481..6530
                     /gene="thiL"
                     /locus_tag="PKK81_08925"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNV33040.1"
                     /translation="MPSGRSRRTSSVGSEFGLIRLLQAQAARPDRQVFKGIGDDTAIL
                     KTSSHEWTLITTDLLAEGVHFNPATSYYEDIGYRAAIANLSDIAAMGGTPRFMLVAIA
                     IPPTCSTEQIQRLYRGMMQAADPYRVCLVGGDTSASRYGLFLSITLTGTVQPRRALLR
                     SGARVGERLYVTGTLGDSRAGLDLLTATARAGAEKLRPTHTRFLLARHHRPSARIAEG
                     QWLVKHGLAGAAIDLSDGLTGDLRHLCEESGVGAEVLAGSLPISPACLAYALAHGRDP
                     QQIGLQGGEDYELLFTVPRRKQEQFEQLAEKTGFCFTCIGSITPKRLGLRLRTAAGTA
                     QPLPVTSYEHFLRPS"
     gene            6554..7033
                     /locus_tag="PKK81_08930"
     CDS             6554..7033
                     /locus_tag="PKK81_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNV33041.1"
                     /translation="MADVRSLFRQVLHLDETPHRTALAFAIGSFIGFSPAYGLHMVMV
                     GFCAWAFGLNVVALLAGAFLNNPWTLIPILGGTYWTGATLLGVHDFPSFDWSDLSFIG
                     IYHQVMPYAWPFFVGGMVLSIAGGLLAYPLAYLLLSKYRRANPTDGHQPLPPSSGLR"
     gene            7055..8476
                     /locus_tag="PKK81_08935"
     CDS             7055..8476
                     /locus_tag="PKK81_08935"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV33042.1"
                     /translation="MKLPTAHPPAPYDGSALIADPIHEYVSFTVPYATPDPSERTEKD
                     LIDSPWVQRLRYIYQLQSARWVYPSAEHTRFVHSLGTMHVAGRFARHLYPFLKKAVPD
                     VPSAALVEEFLRITALVHDIGHGPFCHFFDDNFLHGFGLSHEKLGQIIVREHLGPLIK
                     KIRRSPSGPFDRGEELNPDHIAHVILKEKGKDNSRIPRWINMLQPVISGSYTADNLDY
                     VLRDAYMCGVAVGPVDLTRLIHYTIVTDKGFTIHKTGLPALQMFLNTRMYLYSNVYYH
                     RTTRAIDIHLRDIFGDTMKLLFPSNPAKHMDEYLTLTDWSLLEDVRRWKKAGQSSLRR
                     LHQEWAHILGRDVKWKMAYSTVLKEKGIERGMDFPSHQQFQQQIQKALPAKLQTLPFR
                     VDMAPLDPRPDPKDTRGIPLLVFDPGTKGVSTEPLEEFLDLLPTRLVQFRIYALDHHQ
                     DAALSRAAATVLNKTPLSMETNF"
BASE COUNT         1867 a         2766 c         2346 g         1694 t
ORIGIN      
        1 cgaacagact gatgctcccg ttcacatact ccgacaacca tcggctccac gtcgcgctga
       61 cacccgactt cgactcgaag taggaaatgt agttggtcat cgaccgataa atatccagct
      121 gcagggcatt gtacgagtcg ttcacatagg gattgcggaa cgtcaccaat cccaacgacc
      181 gttgctggcc caattgcccg cggatacgac ccaaccagcc gttcccgccg agatttcctt
      241 cggtgatatc cgcgatcgcc accagcttat ccaatgtgct gaatccaccg ccgatgctga
      301 actggccggt cggcttctcc ttcaccttca cgttgagatc gactttatcg acgtcgacct
      361 gttgcggcag aatttcgacg gtctcgaaaa agttgagatt gttcaatcgc tggaaactgc
      421 gtttcagcgc cagggtgtcg accacatcct gctcatccaa ccgcagttcg cggcgcacca
      481 cgttgtcgcg ggtcttgtcg ttgccgttga cgtggatctc gcggatccgc atcatttctc
      541 cctctttcac attgaggaga atcgtggccg tgcgcgaatt gttgtcgggc gtcacgttgg
      601 gcacgacgtc ggcgaacaca taccccttcg cgccgtacag atccgtgatg cgggtgattt
      661 ctccccggat cttggcccgc tgaaaaattt cgcccggtct gatgttcagc ccttcgcgca
      721 attcatgatc ctcgaacacc gtattcccgc ggaacccgac ctcctgcacg atgaacggct
      781 cgccctcaac gatcgaatag ttgatttcga accacttctt gtcctcgctc agttccagcg
      841 tcggctggct gatctgcacg ttcagatagc ccttgttcaa atagacttcc cgcatgcgtt
      901 cgatatcgtt gcccagttcc tcgcgtttca gcacgccggc atcggacagc agcgacggca
      961 ccttcaactg cgtgatgagt ccataccagg gaatccattc ccgggtcgcc gtcaccttaa
     1021 agatttcttc cttggtcacc gcccgcatgc cctcgaaatt gatatggagg actttggcct
     1081 tggtgccttc cttgatgaag taggtcaacc gtttccggtc ctcatccacc gcctggatga
     1141 cgggaatgac ctgacagttg tagtagccgt cctcttgata ggcgagccgg attttttccg
     1201 cgctctcttt ggcctgctgc tggtcgagga acgtctggct cttgatcgtg accttttcct
     1261 tgagcttgtc gtcgctcaat tcctcgttgc cgtcgaacac gatctccgtg atgaagggtt
     1321 tctcccgaac gacaaaaccg acggacacac ctcccgcggc cggttctgtc tccacctgta
     1381 cgtcttcgaa gtagccggtg ccgtacagga tcttgacctg ctcgcggagc gcgtcggccg
     1441 taaaacgatc gttggccttg agcgtgagac gcccctcgat cgcctggagt tcaatgcgct
     1501 tctgcccacg aatggtgacc gacgtgactg tcggcacgcc gtcctgggcg tacagaggcg
     1561 cccctacacc gaacgcgtag agcgccacca gcgcgagaat aagaatcggc acccaccgcc
     1621 tgcctcccga cttggtcacc gcaatccttc cgtaccactt cggaatgcaa gcactggttg
     1681 tcctgaagtt cgagatagga aaccaccgcg ttccgtgtcg cgtctcgtgc gtcgggccga
     1741 ccggccttgc gcaggcactc acgaactgtt ttcgttacca tcaccgatgc gccgctttcg
     1801 ttgcagcacc accgcacatg tgcagaaaaa ctctcggatt atattgacca catttttcaa
     1861 tgtaaaggag tttccccgcg caacgacgca cgcgccgcgc cgaaaagacc ggaggaaatc
     1921 ttgacttcac ttaccccatc acatactatc cccctatgac gagaacagat gtacgtaaag
     1981 ctattattcc cgctgccgga ctgggcactc gcttccttcc cgccaccaaa gcctctccca
     2041 aagagatgct gccgctggtc gacaaaccgc tgattcagta tgtcgtggag gaagccgtcg
     2101 catccggtat cgaagacatc atcgtcatca cgggtcgcgg caagcgcgcg atcgaagacc
     2161 acttcgaccg ctccctggaa ctggaagaaa atctcaaggg cagcggcaag gggcaggtgc
     2221 tcaaccagat gcggcacatt tcgaatctgg cgaacttctg ttacgtccgc cagccggaag
     2281 ccatgggcct cgggcacgcc gtgctctgcg cccagcacct gatcggcgat gagccgttcg
     2341 ccgtcatttt gggcgatgaa atcatcgacg cggcggtccc cggcctcgcg caactcattc
     2401 atatttacaa gcaacgcaaa ggcgccgtct taggcgtaca ggaagttcca ccccaagagg
     2461 tgagccgata cggcatcatc tctgcccgac gcctgggcaa ggggctgcac cgggtcgatg
     2521 acctggtcga gaaacccgcg cccgccgatg cgccgtcgaa tctcgccgtc atcggccggt
     2581 atgtcttgcc gccggagatc ttcccgatcc tccgcaagac gcctccaggt aagaacggag
     2641 aaattcagct gaccgacgcc ctgcggcaac tcgtcaagca caccccgatg ttcgcccacg
     2701 aaatcgaggg acagcggcac gatgcgggcg acaaactcgg attcctcatt gccaccgtgg
     2761 aattggcgct caagaaccca tctttgggac ctgcgttcgg cgagttccta caacaacgac
     2821 tccgaccgac cgccgccgac gtgcgtcgcc gcggcccaag ccgatccggc caaggtcgaa
     2881 cacgcaccac caactagcct tgggcatgac ggactaccgt cacccagacc gtgccacagc
     2941 acgtcaccat cactgagacc ctacagcagc agactggagc ctcatggctg aagcagtaga
     3001 acaagacttc tcaagcaacc agaacctcga accgccggac cagctgcccc tgctgcctgt
     3061 ccgagatatc gtcgtgtttc cttacatggt gctcccgctg tttgtcggac gcgagatgtc
     3121 gatcaaggcg atcgaagccg cgctcgcggg caaccgcatg ttgttcctcg ccacccagaa
     3181 atccctggat gtggaaaacc cgcaaccgga cgacattcac gccgtcggca ccgtgggcat
     3241 catcatgcgg atgctcaagc tgcccgacga gcggatcaag attttagtcc agggactcgc
     3301 caagggacgg atcgaagaat acatccagaa cgacccctat tattcggttc gcatcgagaa
     3361 actcgtcgag acgaagcagg ccggatccac actggagacc gaggccgtca tgcgcacggt
     3421 caaggagcag atcgagaaga tcgtcagcct cggcaaggtg ctgattcccg acgtgatggt
     3481 ggtcatcgaa aatctggaag accccggccg gctcgccgac atggtggcct ccaatctcgg
     3541 actgaaggtc gacatcacgc aggccgtcct ggaaatcgtg gacccgatcc agcggctgcg
     3601 ccagatcagc gaaattctct ccaaggaaat cgatgtcctc tccatgcagc agaagatcca
     3661 ggcgcaagcc aaaggggaga tggacaagac ccagcgcgaa tacttcctgc gcgagcagtt
     3721 gaaagccatc cagaaagaac tcggcgagtt ggacgaacgg gcggaggaag tggcggaatt
     3781 ccgcaagcgc atcaaagacg ccaagatgcc cgagaaggtg ctcaaggaaa ccgagaagca
     3841 gctcaagcgg ctcgaaaaaa tgcatccgga cacggcggag tcagccacgg tccggaccta
     3901 tctggaatgg atggtcgagc tcccgtggaa taaaaaatcg aaagacaatc tcgacctgaa
     3961 agccgcgatg aaggtcctga acgaggacca ctacgacttg gaaaaggtga aagaacgcat
     4021 cgtcgaatac ctcgccgtgc gcaagctcaa agagaaaatg aaggggccca tcctctgttt
     4081 cgtgggaccg cccggcgtcg gcaaaacctc gctcggcaaa tccatcgccc gcgcgctcgg
     4141 ccgcgagttc gtccggatca gtctgggcgg agtccgcgac gaggccgaaa tccgcggcca
     4201 tcgcaggacc tacgtcggcg cactgccggg acggattatc cagggcatga aacaagccgg
     4261 cacgaacaat cccgtgttca tgctcgacga ggtcgacaaa gtcggcatgg atttccgcgg
     4321 cgatccttcg gccgcgctgc tggaagtgct ggatcccgaa caaaacagca ccttcaccga
     4381 tcactatctc ggggtgccct tcgacctgac cgaagtcatg ttcgtcacga cggccaacct
     4441 catggatccg attctgcccg cgttacggga tcgcatggaa gtcatcgaca tcccgggata
     4501 tacggaagaa gagaaactcg ggatcgcgca gaagtactta attcccagac agatgaacga
     4561 gcacggcatt accgaaaaac atatccgcgt gaacgaggcg accatccgcc acgtcatctc
     4621 gcactacacg cgcgaggccg gagtccggaa cctcgaacgg gaaatcgcca acctcatgcg
     4681 gaaagtggcc aagaaggtgg ccgagggcaa atccgagtgt cacaccatcg accagaccaa
     4741 tttgaacaag tttctgggtg tggcgaagtt cgttcccgag gcggagcttg aaaaagacga
     4801 aatcggcgtc gccacaggcc ttgcttggac ggaaagcggc ggcgacgtgc tgtatatcga
     4861 agcgaccgtc atgaagggca aggggcaact caccctcacc gggcatctcg gcgacgtcat
     4921 gaaagaatcc gcgcaggccg ccctgagcta tgtccgctcg cgagagaaga ccctgggcat
     4981 cagccccgac atcttcgcca agaacgacat tcacattcac gtgccggccg gcgccacacc
     5041 gaaagacgga ccgtctgccg gcattaccat ggccacggcc atcgcttcgg cgctggccca
     5101 gatccccgta cggcgcgatc tcgcgatgac cggagaaatc accctgcgcg ggcgcgtctt
     5161 gccgatcgga ggcttgaagg aaaaaatcct cgcggccaaa cgggcgaaac tcgcgaccgt
     5221 cgtccttccg aagcgcaaca agaaggacct ggaggaaatt cctaaacata tcctcaaggg
     5281 catcgagttg atctttgtcg atacggtcga cgatgtcatc aaggcggccc tgcggcgatc
     5341 atcgtcccgc aaaccgcgcg catcggcccc gaagactccg gtcaagaaat cggcgcgcgc
     5401 gaccacatcc aagcgaacaa ccggctcgcc ccggaaaagc ctccgccttc cgacggcttc
     5461 gccatcgttg attctctaat atgccgtcag gccgatcccg ccggaccagt tctgtcggta
     5521 gcgagttcgg cctgatccgg ctgctgcaag cgcaggccgc ccgacccgac cggcaggtgt
     5581 tcaaggggat cggcgatgac acggccatcc tcaaaacctc gtcccacgaa tggactctca
     5641 tcaccacgga cctcctggct gaaggtgtgc acttcaaccc tgccacctcc tactatgaag
     5701 acatcggata ccgggcggcc atcgccaact tgagcgatat cgccgccatg ggcggcaccc
     5761 cccgattcat gctcgtggcc attgcaattc cgccgacctg ctcgacggaa cagattcaac
     5821 gactctatcg cggcatgatg caggccgccg atccctatcg ggtctgcctc gtcggcggag
     5881 acacgtccgc ctccaggtac ggcctgtttc tcagcatcac gctcaccgga accgtacaac
     5941 ctcgacgcgc tctcctgcgc agcggcgccc gcgtcggcga acggctgtac gtcaccggca
     6001 cactcggcga ctcccgcgca gggctggacc tcctgacggc cacggcccgc gccggagcgg
     6061 agaaactgcg tcccacacac acccgctttc tgctcgcgcg tcaccaccgg ccgtccgccc
     6121 gcattgcaga aggccaatgg ctggtgaaac atggactcgc cggtgcggcg atcgatttgt
     6181 ccgatggtct gaccggtgac ctccgacacc tctgcgaaga gagcggcgtc ggagcggaag
     6241 tgctcgccgg ctcactcccg atttctccgg cctgtttggc ctacgccctg gcgcatggcc
     6301 gtgatccgca gcagatcggg ttacagggcg gcgaggacta cgagctcctg ttcaccgttc
     6361 cgcgccgcaa gcaagagcaa ttcgaacagc tggcggagaa gaccggcttt tgcttcacct
     6421 gtatcggctc gatcactccc aagcggctcg gtctgcgctt gcgaaccgca gcaggaaccg
     6481 cgcaaccgct gccggtcacc agttatgaac attttctgcg gccctcttag cgatatccgg
     6541 gacaagaaca tcgatggcag acgtacgatc gttgtttcgc caagtgctcc atctcgatga
     6601 aacaccgcat cggacggcgc tggcctttgc catcggcagc ttcatcggct tttctccggc
     6661 ctatggtctt catatggtca tggtggggtt ctgcgcctgg gccttcgggc tgaacgtggt
     6721 ggcgctgttg gccggcgcat tcctcaacaa tccctggacg ctgatcccca tcctcggagg
     6781 cacctattgg accggcgcca ccctattggg agtgcatgac tttccgtcct tcgactggag
     6841 cgatctgtcc ttcataggga tttatcatca ggtcatgccc tacgcctggc cgttcttcgt
     6901 cggcgggatg gtcctgagta tcgcaggcgg cctgttagcc tatccgctcg cctatctctt
     6961 gctctccaag taccggagag caaacccgac ggacgggcat caaccgttgc ccccttcatc
     7021 aggtctgcgc taagatcatg catccgccga ccgcatgaaa ctaccaaccg cacatccccc
     7081 cgctccctat gacggatcag ccctcatcgc cgatcccatc cacgaatacg tgtcgtttac
     7141 ggtgccgtac gcgacgccgg atccctcgga acgcacggaa aaagatctga tcgattctcc
     7201 atgggtgcag cgactgcgct acatctatca actccaaagc gcgcgctggg tctacccctc
     7261 agcggaacat acgcgattcg tgcactcgct cggcaccatg catgtagcgg gccgttttgc
     7321 gcgccatctc tatcccttct tgaagaaggc ggtgcccgac gttccctcgg cggctttggt
     7381 cgaggagttt ctacgcatca cggctctcgt gcatgacatc ggacatggtc cattctgcca
     7441 tttcttcgac gataactttt tgcatggttt cggtctctcg catgaaaagc tcggtcagat
     7501 catcgtgcgc gagcacctgg gccccctcat caagaaaatc cgccggagtc cgtcgggccc
     7561 gttcgatcgc ggagaagagc tgaatccgga tcacattgcg catgtgatcc tgaaggagaa
     7621 aggcaaggat aactcccgca ttccgcgctg gatcaacatg ctccagccag tgatttcagg
     7681 cagctacaca gccgacaacc ttgattacgt cttgcgcgat gcgtacatgt gcggtgtcgc
     7741 ggtggggccg gtagacctga cccgtctgat tcactacacc atcgtcaccg acaagggctt
     7801 caccattcat aagaccgggc tgccggccct gcagatgttt ctgaacacca gaatgtatct
     7861 gtattcgaac gtgtactatc accgcaccac ccgcgccatc gacattcatc tgcgcgacat
     7921 cttcggggac acgatgaagc tgttgttccc gtccaaccca gccaagcaca tggatgaata
     7981 cctgaccttg accgactggt cgttgttgga ggatgtcagg cgatggaaga aagccggcca
     8041 aagcagcctg cgtcgtttgc atcaggaatg ggcgcatatc ctgggacgcg acgtgaagtg
     8101 gaaaatggcc tacagcacgg tgctcaagga aaaaggcatc gagcgaggca tggatttccc
     8161 cagccaccag caattccagc agcagattca gaaagcgttg ccggcaaaat tgcagacatt
     8221 gccgtttcgc gtcgacatgg ccccgctcga tccgcgcccg gatcccaagg atacgcgcgg
     8281 catccccctg ctcgtcttcg atcccggcac gaagggtgtt tcaactgaac cgctggaaga
     8341 gtttttggat ctgctcccca cccgcctcgt gcaattccgt atctacgctc tcgaccacca
     8401 tcaggacgca gccctctccc gcgccgccgc gaccgtgctg aataagaccc cattgagcat
     8461 ggaaacgaac ttctaggtcc catcctgctt ccggcaagac tatcggccgg tgcctgtacg
     8521 gccgccgcgc aagaatccgg cagtcttctt atacgcagat accgcaccta ggtaaatgcc
     8581 taggtcggat tgactctact cgcatgaggt aattaggtgc atagtagcgt cgattgcgat
     8641 agcctcttgt aatactggct ccttatgaga ttc
//
LOCUS       DAOQRM010000102         8587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_7717_length_8587_cov_13.489920, whole genome shotgun sequence.
ACCESSION   DAOQRM010000102 DAOQRM010000000
VERSION     DAOQRM010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7717_length_8587_cov_13.489920"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..2762)
                     /gene="glnD"
                     /locus_tag="PKK81_08940"
     CDS             complement(78..2762)
                     /gene="glnD"
                     /locus_tag="PKK81_08940"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNV33043.1"
                     /translation="MSHAVNADSHTAQDAAPASVSQLLVEQRQAIRQRLLAGASGDEV
                     VTATTDMVDGLIVGRYRTAARTGGEALTTAGFQHCCLVAIGGYGRRELAPYSDIDLMF
                     LFHPDAAKVMPELVKQVLHPLWDSGFQVGHSVRTIQDCVDLGLADLTVRTSMMEARFL
                     AGSADLFQQFHARYVRKVVAHGFDTYLDQKVTERQREYQKFGETVYLLEPNVKKSKGG
                     LRDLHLLQWIGAARFHTATIRELSDRGILSQADYRAIKEAKEFLLRVRSFLHTRAGMA
                     QEILTFDEQVWLATHLGFTDRPHLLAVEQFMQQYYRHTMGLHAALMRFVERCRRRSLW
                     QRVVRWLPSPRIDQYFVVDGEALTVPVELRSQVLDRPASLLTLFDLARSRRLNIDPSL
                     LEDIHRQAETLSAEQFHTPETGRTFLSILAGPGTARTLEAMHRAHLLEKLVPAFSRVR
                     GLMQFNQYHKYTVDEHSLLAVAKAEALAEHTGVLGEVYREIKRKDLLHLALLLHDLGK
                     GHEEDHSEVGKRLAEETAARLGFDERDSRTLVMLVHRHLLMAHTAFRRDPYDEKVLLP
                     FAREVGTPEVLRKLLALTAADIAAVGPDVLTKWKESLLIELYLRAMQEVSGERETADE
                     PQRLARIADDVASQQGADRDLLSDKAWIRSELEQFPLRYVYGTSPRRIAAHLGAIRRL
                     QVGGVVVETEFNAPLGTCEYAVIAHDDLIPGLFSKIAGVMAAGGLQILDAQIVTRKDG
                     VVVDTFQVADPDYQGAPPADRCESIAGTITEVLTGRQAIETLMRRGTRLNMGRSLPAH
                     RQPSEVRIDNETSDRFTILDVFADDRQGLLYIITNAIFQLGLSVHASRISTRLDQVAD
                     VFYVTGMDGKKVEEAGRLESIRASILNEIELFLGAHAA"
     gene            complement(2765..3103)
                     /locus_tag="PKK81_08945"
     CDS             complement(2765..3103)
                     /locus_tag="PKK81_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNV33044.1"
                     /translation="MKLIEAIVKPFKLDEVKDALLEIGIQGMTVTEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKIEVAVNDSQVQRVLETITRAAKTGSIGDGKIFVRELTSAVRI
                     RTGETGESAL"
     gene            complement(3211..4542)
                     /locus_tag="PKK81_08950"
     CDS             complement(3211..4542)
                     /locus_tag="PKK81_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNV33045.1"
                     /translation="MVKRSAQRKTVVVGVAGVLILLAESWGTQVWAQNTPLKVDTGDT
                     AWVLVSSAFVLAMLMPGLALFYGGLVRTKNVLGTIMQSVMILSVVSLLWILFGYSLAF
                     GPDKGGVIGGLEWVGLSGVGSEPHPVYGPTIPHQAFMLFQLMFAAIAPALITGAFAER
                     KRFTSVILFAALWSVFVYVPLAHWIWGGGWLAKLGALDFAGGAVIHISSGAAALVCAI
                     VLGKRRGYGTDYMAPHNLPMTLLGTGFLWFGWFGFNGGSALGANGIAVSAIIATHAAA
                     AMGAIAWCGAEWAHRGKPTVLGVASGAVAGLATVTPAAGYIAPMSAIAIGLVAGMTCY
                     AAIVWKGRFGYDDSLDVVGIHGVGGVIGILATGLFASKVVNPGGADGLFFGNPGLFGI
                     QLLVAAVTTIFSIIGTFVILKLVDSMTGLRVSSEEEATGLDLSQHNERAYS"
     gene            complement(4617..5495)
                     /locus_tag="PKK81_08955"
     CDS             complement(4617..5495)
                     /locus_tag="PKK81_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33046.1"
                     /translation="MNMLVGRKARTWVLGLALLAGGGGGALGEAQAGTTDDLMDTTKH
                     PVSVTMAMQADSFFGFNPSIYGTYGLTDNIALAFNFTYWTMINGVGVHDNAPWLETDM
                     GLNFTFLEKRLSVTPMIGFVHGQLLSSRGGFFPNGGGSVNERTTAFEGVVPNIIANYT
                     DDRFEGEFYLGYYKAIRSEGGSGGGGATGCTGLVDTNCLGASQTGGRGTWDFLHYWVS
                     AGMRVNSMWSLGAHYEHLLTTRDSSAPGAAQDYYRWIGPYVEMKLPGNMAFRFTVGKD
                     LTDNQDFYKLKFTKTF"
     gene            complement(5632..>7044)
                     /locus_tag="PKK81_08960"
     CDS             complement(5632..>7044)
                     /locus_tag="PKK81_08960"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNV33047.1"
                     /translation="RILATYRKRHLPNYGVFDESRYFHPGSRLPLLVLNGATIGVNIC
                     EDIWFSDGPTRAQAAAGAEVIVNINASPFHVGKGRMREQVLATRARENRVIVTYTNTV
                     GGQDELVFDGCSMIVDQAGEIAGRGKAFDQDLIVADLDVAAVGRARLAQGRKKPLPPR
                     VAALIDRVDVRLPARKSQSVVVPDLEPPLGRLDEAYRALVLGVQDYVRKNGFKRVVIG
                     LSGGVDSAITAVIAVDALGAENVLGVFMPSPYTSRASREDVADLASRLHIQVDTLSIT
                     ATFKSYLRALSRSFKGRRPDTTEENLQARIRGNLLMAYSNKFGHLVLTTGNKSEMSVG
                     YATLYGDMAGGFAVIKDVPKTMVYELSYMRNQVGPAPVIPKRVLERAPTAELRPDQKD
                     EDSLPPYAILDPILKAYVEEDRALEDIAAMGFDRETVARVIALVDRSEYKRRQAPLGI
                     KITHRAFGKDRRMPITNGYR"
     assembly_gap    7045..7144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7145..7556)
                     /locus_tag="PKK81_08965"
     CDS             complement(<7145..7556)
                     /locus_tag="PKK81_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNV33048.1"
                     /translation="MRSLRIAMAQINPTVGDIVGNTRLIQTWIKEARQAKADLVAFPE
                     LAITGYPPEDLLFKPRFIEDTHRALKAVAAEARGVVVVVGYVGQGATAVSASDASSFP
                     LSGRHELYNEAAVLSDRRILATYRKRHLPNYGVFD"
     gene            7885..>8587
                     /locus_tag="PKK81_08970"
     CDS             7885..>8587
                     /locus_tag="PKK81_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33049.1"
                     /translation="MIRRPTPTQSRLSSFRLAWQLALPCLLLCACSLGTNLTPVGQGP
                     LGAVALERQTSRGTTAKYGTPQTFQASHPAHLTASLTGRLLTGLSVSGLDRPGSSMAQ
                     ESYPLFSHEEVEFLAPLIVGALAQAQPDQRVRFTMQDDGLTTQGTLYMHKTTLRVNLS
                     HYRSGSADGQTRPATLRLSFRPPQALVRADVPQPWMIVEPEQPRIAVSLEALSQLPAP
                     SPVEADHNTTAEAAQT"
BASE COUNT         1755 a         2870 c         2277 g         1585 t
ORIGIN      
        1 aaaatctaac acctcacgaa cgttcatccc ctcctccttt caaccgccga taaaataacc
       61 gcaccacacg ctgtcgttta cgccgcatga gccccgagaa acagttctat ctcattgaga
      121 atcgacgccc gaatcgattc cagccgaccg gcctcctcca ccttcttccc atccataccc
      181 gtcacataaa acacgtcggc cacctgatcg agccgggtgg agatccggga cgcatgcacc
      241 gacaacccca actgaaaaat cgcgttcgtg ataatgtaca acaatccctg ccgatcatcc
      301 gcaaaaacat ccagaatggt aaagcggtcg gacgtctcat tgtcgatccg cacctcagac
      361 ggctgccggt gggcaggcaa cgatcgcccc atgttcagcc tggtcccgcg acgcatcagc
      421 gtctcaatgg cctgacgccc cgtcaacacc tccgtgatcg tgccggcaat cgattcacac
      481 cggtcggcag gcggagcccc ctggtagtcc ggatcagcca cctggaaggt atcgacgacc
      541 acgccgtcct ttcgcgtcac gatctgggca tccagaatct gcaatccgcc tgcggccatg
      601 acaccggcga tcttcgagaa caacccgggg atcagatcgt catgcgcaat cacggcatat
      661 tcgcaggttc ccaatggggc attgaattcc gtctccacca ccaccccacc gacttgcaac
      721 cggcgaatgg cgccaagatg cgcggcgatg cgtcggggag aggttccata gacgtatcgc
      781 aggggaaact gttccagctc cgaccggatc catgccttgt cggacaggag atcccgatcg
      841 gctccctgct gtgaagccac gtcgtcggca atacgcgcga ggcgctgcgg ttcgtccgcc
      901 gtctcacgct cgccggacac ttcctgcatg gcccgcaggt ataactcgat caacagggac
      961 tctttccact tggtcaggac atccggcccg actgcagcga tatctgcagc cgtcagggcc
     1021 agcaacttgc gcaacacttc cggcgtgccc acttcgcgtg cgaacggcag caacactttt
     1081 tcatcgtagg gatcacgacg aaaggccgta tgggccatca gcaaatggcg atgcaccaac
     1141 atgaccaacg tccgcgagtc ccgctcatca aaccccaatc gcgcggccgt ttcttccgcc
     1201 aaccgtttac cgacttcgct gtggtcttct tcgtggccct ttcccagatc gtgaagcagc
     1261 aacgccagat gcagcaaatc cttccgtttg atctcccggt agacttcacc gagcacaccg
     1321 gtatgctcag ccaaggcttc ggccttcgcc actgccaaca gactatgctc gtcgaccgtg
     1381 tacttgtggt actggttgaa ttgcatcaac ccgcgcacgc gcgaaaaggc cggcaccagt
     1441 ttctccagga gatgcgctcg atgcatcgcc tccagtgtcc gcgccgtgcc gggccccgcg
     1501 agaatcgaca agaacgtacg gccggtctcc ggggtatgaa attgctcagc cgacagcgtc
     1561 tccgcctgac gatgaatgtc ttcgaggagt gacggatcga tattcaatcg gcgcgatcgc
     1621 gccagatcga acagcgtcag gagcgatgcc ggccgatcca acacctgaga acgcaattca
     1681 accggcaccg tcagcgcctc gccgtcgacg acaaagtact gatcgatccg gggagacggc
     1741 aaccatcgca ccacgcgctg ccacaaggaa cgacggcgac accgctcgac aaatcgcatc
     1801 aacgccgcgt gcagccccat cgtgtgtcga tagtattgct gcatgaattg ctcgacggcg
     1861 agcagatgcg gccggtcggt gaagcccaga tgtgtggcca gccagacctg ctcatcgaac
     1921 gtgagaattt cctgcgccat gccggcccga gtgtggagga acgagcggac gcgcagcaaa
     1981 aattctttcg cctccttgat cgcccgataa tcggcttgcg agagaatccc gcgatcggac
     2041 aattcacgaa tcgtcgcggt atggaagcgc gcagccccga tccactgcag gagatggaga
     2101 tcccgcaacc cgcctttgct ttttttgacg ttcggctcca gaaggtagac tgtttcaccg
     2161 aacttctggt actcgcgctg ccgctcggtc accttctggt cgaggtaggt atcgaatccg
     2221 tgcgccacga ctttccggac ataccgcgca tgaaactgct ggaacaggtc ggcgctgccg
     2281 gccaggaagc gggcctccat catcgacgtc cggaccgtca gatcggcgag gcccaaatcc
     2341 acacagtctt gaatagtccg tacgctatgg ccgacctgga atccgctgtc ccacaacggg
     2401 tggagcacct gcttgaccag ctcgggcatc accttcgcgg cgtccgggtg aaacagaaac
     2461 atcaggtcga tatcggagta cggcgccaat tcccgccggc catacccgcc gatcgccacc
     2521 aggcaacaat gttgaaagcc agccgtcgtg agcgcctcgc cgccggttct cgccgcagtc
     2581 cggtagcggc cgacgatcaa tccatcgacc atgtcagtgg tcgccgtgac cacctcgtcg
     2641 cccgaggcgc ccgcgagcag ccgttgccgg atcgcctgtc gttgctcgac cagcagctgg
     2701 gataccgacg cgggggccgc gtcctgggct gtatgagaat ccgcattcac agcgtgcgac
     2761 atacctagag ggcgctttcc cccgtctcgc ccgtacgaat cctgacggcg gaggtgagtt
     2821 cccgcacaaa gattttcccg tcgccgatgc tgccggtttt ggcggcacgc gtaatcgttt
     2881 cgagcacacg ctgcacctgg ctgtcattca cggcgacctc gatcttcacc ttcggcacga
     2941 actcgatggt gtattcctgg ccgcgatacg tttctttatg gcccttctgg cggccgaagc
     3001 ccttaacctc cgtgaccgtc atgccctgaa tgccgatttc cagcagggcg tccttcacct
     3061 cgtcgagctt gaatggtttg acgatggctt cgattaattt catccttgcc tccagggagt
     3121 agctctcagc cgtcagcagt cagctctcag gcgaggaaag agtcctgaca atcttgctga
     3181 cgactgatgg ccgattgcgc tgagctggct tcacgaatac gcccgctcgt tatgttggct
     3241 caagtccaaa ccggtcgctt cttcctcaga cgacacccgt agcccggtca tagaatccac
     3301 cagcttgaga atgacaaacg tgccgatgat cgagaaaatc gtcgtgacgg ccgcgaccag
     3361 gagttggatg ccgaagaggc cggggttacc gaagaacaat ccatcggcgc cgcccggatt
     3421 gacgaccttg ctggcgaaca gtcccgtcgc cagaatcccg atcacccccc cgactccgtg
     3481 gattcccacg acgtcaagcg agtcatcata gccgaatcgc cctttccaga caatcgcggc
     3541 gtagcacgtc atgccggcga ccaaaccgat ggcaatggcc gacatgggtg cgatatagcc
     3601 ggccgccggg gtcacggtcg ccaatcctgc caccgcgccg ctggcgactc cgagcaccgt
     3661 gggtttgccg cgatgcgccc attcggcgcc gcaccaggca atggccccca tcgccgccgc
     3721 tgcatgggtc gcgataatcg cggagacggc gatcccgttg gcgccgagtg cgctgcctcc
     3781 attgaaccca aaccagccga accagaggaa tccggttccg agcaatgtca tcggcagatt
     3841 gtggggcgcc atataatccg tcccgtaccc tcgccgcttc cccagcacaa tggcgcagac
     3901 cagggccgcc gccccggaac tgatatggat caccgcgccg ccggcgaaat ccaaggcacc
     3961 cagcttggcc agccagcctc cgccccagat ccagtgcgcc aggggcacat agacgaacac
     4021 cgaccacaag gccgcaaaca agatgaccga cgtaaaccgc ttcctctcgg caaacgcgcc
     4081 ggtgatcaac gccggtgcga tggcggcgaa catcaattgg aacaacatga aggcctggtg
     4141 cggaatcgtc ggtccataga ccggatgggg ctcactaccc accccgctga gcccgaccca
     4201 ttccaagcca ccgatcaccc cgcccttatc cggcccgaac gccaggctat acccgaacag
     4261 gatccacagc agactcacca cactcaggat catgacactt tgcatgatcg tgccgagtac
     4321 gttcttggtc cgtaccaggc ccccgtaaaa cagcgccagt cccggcatga gcatcgccag
     4381 cacaaaagcc gaggacacca gcacccatgc cgtatcaccg gtatcgacct tcagcggcgt
     4441 attctgcgcc cagacctgag tcccccatga ctccgccagc aggatcaaca cccctgccac
     4501 gcccacgacg accgttttcc gctgtgccga tctcttcacc atatcctcct tcggctgcgc
     4561 cgctcaccga tcggcctcct ccctgactca gcagaggagg aggcccgggc gagagactaa
     4621 aacgtcttcg tgaacttcaa cttatagaaa tcctgattgt ctgtcaggtc tttacccact
     4681 gtgaaccgga aggccatgtt ccccggcaat ttcatttcga cgtacggtcc gatccagcga
     4741 tagtagtcct gagccgcccc gggggcgctg ctgtctctcg tcgtcaacaa atgctcgtag
     4801 tgggccccga gggaccacat gctgttcacg cgcattccgg cactcaccca ataatgcaag
     4861 aagtcccagg tccctcggcc accggtctga ctggcgccca agcaattcgt gtcgacgagt
     4921 ccggtgcaac cggtcgcacc gccgcctccg ctgccccctt cactccggat ggctttgtag
     4981 tagcccagat agaactcgcc ttcgaagcga tcgtccgtat aattcgcgat aatgttggga
     5041 acgacgcctt caaacgccgt cgttcgctcg ttcacactcc caccaccgtt gggaaagaag
     5101 ccgccgcgag aagagagcaa ttgcccgtgc acgaacccaa tcataggagt gaccgataac
     5161 cgcttttcca ggaaggtaaa attcaagccc atatcagttt caagccaggg cgcgttgtcg
     5221 tgcacaccca cgccgttgat catggtccaa taggtgaagt tgaaggccag ggcgatgttg
     5281 tcggtcagtc cataggtccc gtagatggag ggattaaacc cgaagaaact gtcggcttgc
     5341 atcgccatgg tcaccgacac ggggtgtttg gtcgtatcca tcaggtcgtc tgtcgtcccg
     5401 gcttgcgcct cgccgagcgc cccacctccg ccccccgcaa gtaatgccaa ccccaacacc
     5461 catgtccgtg cctttcggcc aaccagcata ttcatgacgc ctccaaagag agaagaagta
     5521 aaagtgcccg ctcagaagac cgtcgtacag aacggtctat gagccccgtc tctctggcgc
     5581 cattgccagt gttgccgacc gacgtcgttg tcggtcctga tgcggcccgt actaccggta
     5641 cccgttggtg atcggcatcc gccgatcctt tccgaacgct cgatgcgtaa tcttgatccc
     5701 cagcggcgcc tgccgccgct tgtactcact ccgatccacc agcgcaatca cccgcgcgac
     5761 cgtctcccga tcgaacccca tcgctgcaat gtcttccaac gcgcggtcct cctcgacgta
     5821 ggccttgaga atcggatcga ggatggcgta gggcggcaaa ctgtcttcgt ccttttgatc
     5881 ggggcgtaac tccgcggtcg gagcccgctc aagcactcgc ttcggaatca ccggggccgg
     5941 cccgacctga ttgcgcatgt acgacagctc gtagaccatg gtcttcggca catccttaat
     6001 caccgcaaac cctccggcca tatcgccgta cagcgtcgcg tatccgacgc tcatttcact
     6061 cttattgccg gtggtcagca ccagatgacc gaacttattt gaataggcca tcagcagatt
     6121 gcccctgatc cgggcctgta gattctcttc ggtcgtgtcc gggcgccgtc ccttgaagga
     6181 tcggctgagg gcgcgcaggt aactcttaaa cgtcgccgta atggacaggg tatcaacctg
     6241 aatgtgcagc cggctggcca gatcggcgac atcttcgcgg ctggctctgg acgtataggg
     6301 cgagggcatg aacaccccga gcacattctc agcgcccaag gcgtctaccg cgatgaccgc
     6361 tgtgatggcc gagtccaccc cgccgctcaa tccgatgacg acgcgcttga acccgttctt
     6421 gcggacatag tcctgcaccc cgaggaccaa ggcgcgatag gcctcgtcga ggcgacccag
     6481 cggaggctcc agatccggca ccacgaccga ttgcgacttt ctcgcaggca agcgcacatc
     6541 gacccgatca atgagggccg ccacacgcgg cggcaggggt ttcttgcggc cctgggccag
     6601 tcgcgcccgc cccacggccg cgacatcgag atccgcaacg atgagatcct gatcaaacgc
     6661 cttcccccgc cccgcgatct cgccagcctg gtccacaatc atgctgcagc cgtcgaaaac
     6721 caattcatcc tgcccgccga ccgtgttggt gtacgtcacg atcacgcgat tttcacgggc
     6781 cctggtcgcc agcacctgtt cgcgcatccg tcccttcccg acatggaacg gggatgcgtt
     6841 gatattgacg atgacctcgg cccccgctgc agcctgcgca cgggtgggac cgtcagaaaa
     6901 ccagatgtct tcgcaaatat tgacgccgat cgtcgcgccg ttcaggacca gcagcggcag
     6961 acgggagccg gggtggaaat accggctctc gtcgaacacg ccgtagttcg gcagatgccg
     7021 tttccggtag gtggccagaa tccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnncgtcga acacgccgta gttcggcaga tgccgtttcc ggtaggtggc cagaatccgg
     7201 cgatcggaca gcaccgccgc ctcgttgtat aactcatgcc gaccggacag cgggaacgaa
     7261 gaagcgtccg acgccgaaac agccgtcgcc ccctgcccga catacccgac gaccacgacc
     7321 accccccgcg cttccgccgc gaccgccttc aatgcacgat gcgtgtcctc gatgaaacgg
     7381 ggcttgaaca acagatcctc cggcggatag ccggtgatgg ccagttccgg gaaggccacg
     7441 aggtcggcct tggcttggcg cgcttccttg atccacgtct ggatcaagcg cgtgttgccg
     7501 acaatatccc cgacggtcgg attgatctgg gccatggcaa ttcggagcga ccgcatatct
     7561 aaagcctgta agccctcagc gttcagcaat cagcctgaac acgagtcatc ggaactttaa
     7621 gatcctgagc tgacgggatt catcagcatg ctgataaaaa aaacgtcctc agacccacct
     7681 cggtggatcg gaggacgtcg ttgtcctgct tccaatgtat cgttcggaac gaattactcg
     7741 tcggagacgc cattgtcccc ggaaattatc ccgtgtgtat accaccggcc taaacaggct
     7801 gtcaaccaat tgaatcgcac ttttccaggg gaaaccaggc ggttccgcgg tcagcctgtg
     7861 gtacaatcgg ccgccatgcg gttcatgatt cgccgtccca ccccaacaca atcacgactc
     7921 agcagtttcc ggctcgcctg gcagctggcg cttccctgcc tcctcctctg cgcctgcagc
     7981 ctcgggacga acctgactcc tgtcggacag ggccccctgg gagccgtcgc tcttgaacgc
     8041 cagaccagcc gtggtacgac cgctaaatac ggcaccccgc agacttttca ggcgtcgcat
     8101 cctgcccatc tgacggcctc gctgacggga cgcctgctca ccggcctgtc cgtttcagga
     8161 ctcgatcggc cgggaagcag catggcgcag gagagttatc cgctgttctc tcacgaggaa
     8221 gtggagtttc tggccccgct catcgtcggc gcactcgcac aggcccaacc tgaccagcgg
     8281 gttcgcttca ccatgcagga tgacggtctc acgacccaag gcacactcta catgcacaag
     8341 accaccctgc gagtgaacct gtctcactac cgatcggggt ccgcggatgg acagaccaga
     8401 cccgcaacac tcaggctctc cttcaggccc ccacaagccc tcgtgcgagc cgatgtccct
     8461 caaccctgga tgatcgtcga gcctgaacag ccgcgtatcg ctgtctccct tgaggcgctg
     8521 agccagcttc ctgcgccatc ccctgtcgaa gccgaccaca acacaactgc cgaggcagcg
     8581 cagacac
//
LOCUS       DAOQRM010000103         8547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_7790_length_8547_cov_15.279557, whole genome shotgun sequence.
ACCESSION   DAOQRM010000103 DAOQRM010000000
VERSION     DAOQRM010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7790_length_8547_cov_15.279557"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /gene="ybgF"
                     /locus_tag="PKK81_08975"
     CDS             <1..372
                     /gene="ybgF"
                     /locus_tag="PKK81_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNV33050.1"
                     /translation="ESYERTLTRFKDGDLDGARQGFAEFLLQHPHSDLAPNARFWLGE
                     SYYGKKDFSRAIDAYDQVQLNHPASEKVPAALLKKGYAYLALKDRKKAASALKQVIDL
                     YPKSPEANKAMDKLNQLKELH"
     gene            375..1154
                     /gene="ybgF"
                     /locus_tag="PKK81_08980"
     CDS             375..1154
                     /gene="ybgF"
                     /locus_tag="PKK81_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNV33051.1"
                     /translation="MKLRFVEALQVGAGLTCGLVLAGCAKHADFLEIRDQVSIIARTQ
                     DQEQKRFEAMQRRLESLERVREPEGGKLRLDDALARLQKLEGRLAKIEETQLAQAASI
                     RSDLALAEANRQARVSKPSGPIEPATIMPGVPSITPTSAFNLAYNDYLNGKFDLAVTG
                     FQRFIKDFPSTSLTPNAHYWLGESYYGQKDYIRAMQSFEHVVNEYAGNEKVPAALFKL
                     GLSAAETGDTAKSRKYLKRVIEEYSTSDEAKLAKAKMAEIR"
     gene            1227..>2698
                     /gene="mutL"
                     /locus_tag="PKK81_08985"
     CDS             1227..>2698
                     /gene="mutL"
                     /locus_tag="PKK81_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNV33052.1"
                     /translation="MDIASSVGKIQVLPSDVIGRIAAGEVVERPAAVVKELVENSLDA
                     GSSTITVEVKDGGLGLIRVTDDGEGMSRRDASLAFERHATSKLQSDQQLGTIRTMGFR
                     GEALPSIAAVSKVRLTTLSRQEQVGTQLWLTAGTITRVEDAAAIPGTSIEVSELFYNT
                     PARRKFLKSTTTEFSHISHVVQQAALASPQVHVRLIHNGYEVFALPAVSSPRDRVLQV
                     YRAAFGDRALAVDVQQNGLSLRGFIIDPVRARAGRTPQELFVNRRPIKNSTVQHAVVD
                     GYSSFLAKGHAPLFVLFLDVEPQRVDVNVHPTKREVRFADNEQIHQLVRSAVRHTLGR
                     AHIESSMAGAAHAHPDGNGVSNVAIPVEETGVEPAALSYPSFAPLNVPVPPTPSAESQ
                     TSFVGEGGTPYQSGEAVDIVPLGQMHRTYVIAQVGDELQVIDQHTAHERVLFERLLRA
                     WQGKTLPSQPLLLPEPLELPVQQALILQRHLAELERLGLL"
     assembly_gap    2699..2798
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2885..3916
                     /gene="miaA"
                     /locus_tag="PKK81_08990"
     CDS             2885..3916
                     /gene="miaA"
                     /locus_tag="PKK81_08990"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNV33053.1"
                     /translation="MVRLSESQRASRPLVLLVGPTAVGKSEIGLRLAHALDTDVLTAD
                     SRQVYRGMDIATDKPTPEQRQGVPHRLIDLVDPDEPFNAGQYRRLALQEIQRLYGAGR
                     LPLIVGGTGLYVRTLIHGLCDAPRADQAYRESLLQEARAQGGYFLHTELTRVDPESAA
                     RLHPHDEVKIVRALEVHHLSGQRLSDMQRQHRFAEQEFSVLLIGLNRNRAQLYRRIDD
                     RVESMFARGVVEETERLLADGYGRELGAMKGLGYQQVAGYLAGEYDRAEALRLLKRDT
                     RHFAKRQLTWFRKEPGLRWCELTEQDRSEDVAGRLLEMIRSFVQDLAPRRPEEMPNPS
                     LTMEQESTA"
     gene            3913..4812
                     /gene="mtnP"
                     /locus_tag="PKK81_08995"
     CDS             3913..4812
                     /gene="mtnP"
                     /locus_tag="PKK81_08995"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNV33054.1"
                     /translation="MTESKKAGQAAIGIIGGSGLYDIEGLERVREVRVRTPFGAPSDA
                     IRVGVLGGIRVAFLSRHGRGHRLSPSSINYRANIYALKSLGVTQVISVSAVGSMKESI
                     RPGDVVLPDQFIDLTKRRVSTFFDEGIVAHVGFGEPVCSSVADVLEQAGRAVGARLQR
                     GGTYVCMEGPQFSTKAESRLYRQWGVDVIGMTNMPEAKLAREAELCYATVALATDYDC
                     WHDTEEAVTVEAILATLHKNVALAKQLLKAALPKLAPDRTCVCHQALRTAIVTAPDCI
                     SATAKRRLNLLIAPSVRTRKGKR"
     gene            4816..5733
                     /locus_tag="PKK81_09000"
     CDS             4816..5733
                     /locus_tag="PKK81_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNV33055.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEVTEVLGGSATYFSTAASYFTSVD
                     LIAVVGEDFPEQHVAFLKSRKIDLAGLERRPGATFRWKGAYSHQLNEAQTLDTRLNVF
                     ETFRPKIPAQYSSPDVLFLGNIDPNLQLDVLQQVKRPALVACDTMNFWINGKRDALWN
                     VLQHIDILIINDGEARALGEDTNLVKVAKKILARGPKHLIVKRGEYGVLMFNDKQVFG
                     APAFPLDDVRDPTGAGDTFAGGFLGYLAATGNRSPEAMRQAIIFGSVMASFTVEAFSL
                     DRLRILDYKEIEARFKEFKRLTHFEDIEA"
     gene            5730..6431
                     /locus_tag="PKK81_09005"
     CDS             5730..6431
                     /locus_tag="PKK81_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV33056.1"
                     /translation="MMTIRREQDRAARTASVGAGARWLVLLLGLCVLSGCANEENLRK
                     SKGFYQEGVARLSSDQQQAYVSFQKAVKLNPDNKEAHYGLGHIYSSQGRFKLAEESFR
                     EAIRIDGDYAEANTYLGQVLANQDRWKEAIAAYRQALSNPLYPTPDLARFHLGKALMH
                     EGDLQGAMEVLEDATTVTPPNVPPAMLQLELARVYHKLGFDVRAREALAKVSNLDKGG
                     EQAAAAQELLGQLKP"
     gene            6441..7394
                     /locus_tag="PKK81_09010"
     CDS             6441..7394
                     /locus_tag="PKK81_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNV33057.1"
                     /translation="MESVGEFFRQVRETKGLTVDEVASKTRIRTDFVKALEEGNFAKL
                     PDQVFARGFVRSYARSLGLDEEDAIHRFVQSAGAFYEKQGERERLRQRQVEEERRRKA
                     NRKAVGIAIAVAIATLVFLLSREQSSTLVRRSGSDQPPTSKKSAPFAKDTREAPRQGL
                     ELPPPIVPPATKPAEPVAVPSKPAQEKVAAAVPPPPAPPVKPAPVQPEQMAAQPSFSG
                     SDGPLAGLSVDGPAGPDGPLVLDLDATELSWVVVQVDSGSPQEALLRPGQKAQWKAQD
                     QFTVTLGNAGGVRAELNGKPQKPFGPSGKVVRDVVLKRSGL"
     gene            7830..8282
                     /locus_tag="PKK81_09015"
     CDS             7830..8282
                     /locus_tag="PKK81_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV33058.1"
                     /translation="MGYFSKLVGVCAAVTLLSVAVVGAEEKDPLKPRVAPDQMADAKA
                     MKNPVASTPESIAKGKALYEGKGTCFNCHGKEGKGDGPAGAILNPSPRNFTNCKFHKK
                     RKDGELFWVIKNGSAGTGMVSLIPAAINEEEAWMIINYERSFCKGGEE"
BASE COUNT         1680 a         2329 c         2700 g         1738 t
ORIGIN      
        1 gagtcgtacg agcggacgtt gacccggttt aaggacggcg atttggacgg ggcgcgccag
       61 ggcttcgcgg aatttctcct gcaacatccc cactcggacc tggctcccaa tgcgcggttc
      121 tggctgggcg agtcctatta cgggaagaaa gatttctcgc gcgccatcga tgcctacgat
      181 caagtgcagt tgaaccatcc cgccagcgaa aaagtgccgg ctgccctctt gaagaagggc
      241 tatgcttatc tcgcgctcaa agatcgaaag aaagccgcct cggcgctcaa gcaggtcatc
      301 gacctgtatc caaaatctcc ggaagccaat aaggcgatgg acaaactgaa tcaactaaag
      361 gagttgcact aacgatgaag ctccgattcg tggaggcatt gcaggtaggg gcggggctga
      421 cctgcggact ggtgttggca gggtgtgcga agcacgccga tttcctggag atccgggatc
      481 aggtctcgat catcgcccgg acgcaggatc aggaacagaa gcgattcgag gcgatgcagc
      541 gccgactgga gtcgctcgaa cgcgttcggg agccggaggg cggaaagctg cggctcgacg
      601 atgcgttggc ccgccttcag aagctcgagg gacgtctggc caagattgaa gaaactcaac
      661 tggcccaagc cgcctccatt cggtcggacc tcgcgcttgc cgaagccaat cgtcaggcgc
      721 gtgtctcgaa gccgtccggg cccattgagc cggcgaccat catgcccgga gtgccgtcga
      781 ttacgccgac ctcggccttc aatctcgcct ataacgacta cctcaacggc aagttcgatc
      841 ttgccgtgac cgggtttcag cggttcatca aggattttcc ctcgacctcg ctgacgccga
      901 acgcgcacta ttggctcgga gaatcgtact acggccagaa ggattatatt cgggcgatgc
      961 agtcgttcga gcacgtcgtg aacgagtatg cgggcaatga aaaggttccc gccgcgctct
     1021 tcaagctggg cttgtcggcc gccgaaaccg gcgataccgc aaaatcgcgc aagtatctga
     1081 agcgggtcat cgaggaatat tcgacgtccg atgaagccaa gctcgcgaaa gcgaagatgg
     1141 ccgaaattcg atgaccttgg gcctgctgca cggtcctctc tccatccgct catcctcggt
     1201 tcgaatctca gggagcgttt cttcccatgg acatagcgag tagtgtggga aagattcagg
     1261 ttctgccgag cgatgtcatc ggccgcattg cggcagggga ggtggtcgag cgtccggctg
     1321 cggtcgtcaa agagctcgtt gaaaacagcc tggacgccgg cagcagcacg atcaccgtcg
     1381 aggtcaaaga cggaggcctc gggctgattc gtgtcaccga cgacggcgag ggcatgtccc
     1441 gacgtgatgc ttctctcgct tttgagcggc acgccaccag taaattgcag tcggatcagc
     1501 agctcggcac cattcgcacc atgggctttc gcggggaggc gttgcccagc atcgcagccg
     1561 tatcgaaagt ccggctcacg acgttgtctc gccaggaaca ggtgggcacg cagctctggc
     1621 tcacggcggg gaccatcacg cgggtcgaag atgccgcggc gattcccggg acatccatcg
     1681 aggtttcgga gctcttctac aatacgcccg cccgcaggaa atttctcaaa tcgaccacga
     1741 cagagttctc ccatatcagc catgtggtgc agcaggccgc gttagcgtcg ccgcaggtac
     1801 acgtgcggtt gattcataac ggctatgagg tcttcgcact gccggccgtg tcctcgcctc
     1861 gcgatcgtgt gctgcaggtg tatcgcgccg cattcggtga ccgggcgctg gccgtcgatg
     1921 ttcagcagaa cggtttgtcc ctgaggggct tcatcatcga tccggtgcgg gcgcgcgccg
     1981 ggcggacgcc gcaagaactc ttcgtcaatc gcaggccgat caaaaacagc accgtgcaac
     2041 atgcggttgt ggatggctac agttcgtttc tcgcgaaggg ccatgctccg ctgtttgtgc
     2101 tctttcttga tgtcgagccc cagcgggtcg atgtcaacgt gcatccgact aaacgagagg
     2161 tccggtttgc cgataacgag cagatccacc agctggttcg ttctgctgtg cgccatactc
     2221 ttggtcgcgc ccacatcgaa tcgtcgatgg ctggggccgc tcacgcgcat ccggacggga
     2281 acggggtatc gaatgtcgcg attcccgtcg aggaaactgg cgtcgagccg gcagccctgt
     2341 cgtatccatc attcgcaccc ctgaacgtgc ctgttccgcc gactccctca gctgagagcc
     2401 aaacctcgtt tgtcggggag gggggcactc cttatcaatc gggtgaggcc gtggatattg
     2461 ttcccctcgg ccagatgcac cgcacctatg tgattgcgca agtgggcgat gaactccagg
     2521 tgatcgatca acatacggcc cacgagcggg tcttgtttga gcgactgttg cgagcctggc
     2581 agggcaagac cttgccgtcc cagccgctgc tgttgccgga accgttggag ttgcctgttc
     2641 aacaagctct catccttcag cgccatctgg cggaactgga acgactcggg ttgctcatnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gcacggccgc cgtgtggcct
     2821 tccgtctctc gggggacgaa ctggctcgcc tgttcgatcg tgcataggaa gagggagttc
     2881 ggggatggtg cgactgtcgg aatcgcagcg agcgtcgcgg cccctggtgc tgctggtcgg
     2941 gccgacggcg gtgggaaaga gcgagatcgg ccttcggcta gcgcatgcgc tggatacgga
     3001 tgtgctgacg gcagattcgc gccaggtcta tcgcgggatg gacattgcga ccgacaagcc
     3061 gacgccggag caacgacagg gcgtgccgca ccgcctcatc gatttagtcg atcccgacga
     3121 gccgtttaac gccgggcagt atcgccggct ggcgctgcag gagattcagc ggctgtatgg
     3181 agccggccgg ttgccgttga tcgtcggtgg cacaggactg tacgtgcgga cactgattca
     3241 cggattgtgt gatgccccgc gcgcagatca ggcctatcga gaatcgttgt tgcaggaggc
     3301 ccgcgcgcag ggcggctatt ttttgcacac tgaattgacg cgtgtcgatc ccgaatcggc
     3361 cgcacggctc catccccacg acgaagtcaa aatcgtgcgg gcgctcgaag tccatcatct
     3421 gtcgggacaa cgcctgtcgg atatgcagcg gcagcatcgt ttcgccgagc aggagttttc
     3481 ggtgctcctc atcggtctga atcggaatcg cgcgcagctc taccgccgaa tcgacgaccg
     3541 ggtcgagtcg atgtttgcgc gaggcgtggt ggaggaaacc gaacgactgc tggccgacgg
     3601 gtatgggcgg gagttgggtg cgatgaaggg gctggggtat cagcaggttg ccggctatct
     3661 ggccggcgaa tacgaccggg ctgaggcgct ccgcctgttg aagcgtgata ccagacattt
     3721 cgccaagcgt cagttgacat ggtttcggaa ggagcccggc ttgcgctggt gtgagctcac
     3781 tgagcaggat cgttcggagg atgtagccgg gcgtctgctt gagatgattc gttcgtttgt
     3841 ccaggatcta gcacctcgac ggccggagga aatgccgaat ccgtcgctta ccatggaaca
     3901 ggaatcgacc gcatgactga atcaaagaag gccgggcagg ccgcaatcgg tattatcggt
     3961 ggcagtgggt tgtacgacat tgagggactg gagcgagttc gggaagtgcg cgttcgaacc
     4021 ccgttcggtg cgccgtcaga tgccattcgt gtcggggtgt tgggtggcat ccgtgttgcg
     4081 tttctctccc gacatgggcg ggggcaccgg ctcagtccca gcagcatcaa ctatcgcgcc
     4141 aatatctatg cattgaaatc gctcggggtg acgcaagtga tttcggtcag cgccgtcggc
     4201 agcatgaagg aatccatccg tcccggtgac gtcgtgctgc cggatcagtt catcgatctg
     4261 acgaaacgca gagtgtcgac gtttttcgac gagggcattg tggcgcatgt cgggttcggg
     4321 gagccggtct gttcgtcggt ggcggatgtg ttggagcagg cagggcgagc ggtgggggcg
     4381 cggcttcaac gcggtggtac ctacgtgtgt atggaaggac cccagttttc tacgaaggcg
     4441 gagtcccggt tgtatcgcca gtggggggta gatgttatcg gtatgaccaa catgccggaa
     4501 gccaaacttg cccgtgaagc ggagctctgt tacgcgaccg ttgcgctcgc gacggactac
     4561 gactgctggc atgacaccga agaggccgtc acggtcgagg ccattctggc cacgctgcat
     4621 aagaatgtcg ctctagctaa acaactgttg aaggcggcgc tgcccaagtt ggcgccggac
     4681 cggacttgtg tctgtcatca ggcgcttcgg accgccatcg tgaccgcgcc ggattgcatc
     4741 tctgccacgg cgaagcgacg gctcaactta ttgattgctc cctctgtgcg gacgcggaaa
     4801 ggaaaacggt gaagcatggg taaattattg gtagtgggat cggtggcgct ggataccgtc
     4861 aaaacgccgt tcggtgaggt caccgaagtg ctcgggggat cggcgacgta cttttcgacg
     4921 gccgcgagct acttcacctc ggtcgatctg attgcggtcg tcggggagga ttttccggag
     4981 caacacgtgg cgttcctgaa gagccggaag atcgatttgg ccggcttgga gcggcgaccg
     5041 ggtgccacgt tccgctggaa gggggcctat tcgcaccaat tgaacgaagc gcagacgctc
     5101 gatacccgct tgaatgtgtt cgagacgttt cgtcccaaga ttcccgctca gtacagttcg
     5161 ccggatgtcc tcttcctggg caacatcgac ccgaatttac agctggatgt cttgcagcag
     5221 gtaaaacgtc ctgcgctggt cgcctgcgac acgatgaact tttggatcaa cggcaagcgc
     5281 gacgcactct ggaacgtgct gcaacacatc gacattctca tcatcaacga cggcgaggcg
     5341 cgggccctgg gagaggacac gaatctcgtc aaggtcgcta agaagattct cgcgcgaggc
     5401 cccaaacact tgatcgtcaa acgcggtgaa tatggcgtcc tgatgttcaa cgacaagcag
     5461 gtcttcgggg cgccggcgtt cccgctggac gatgtgagag atcccaccgg tgcgggcgat
     5521 acgttcgccg gaggatttct cggttacctg gcagcgaccg gaaaccggtc tcccgaggca
     5581 atgcggcaag ccatcatttt cggcagcgtc atggcctcgt ttacagtaga agcctttagc
     5641 cttgaccgat tgaggatcct ggattacaaa gagattgagg cgcgtttcaa agaattcaag
     5701 cggctcactc actttgagga cattgaggca tgatgacaat tcgccgcgag caggatcgcg
     5761 ccgcgcgcac cgcttctgtc ggcgcggggg cacgctggct ggtgttgctc ctgggcttgt
     5821 gtgtgttgag cgggtgtgcc aacgaagaga acctgcgcaa gtcgaaaggg ttctatcaag
     5881 aaggggtcgc ccggctcagt tccgatcaac agcaggccta cgtgtcgttt cagaaggcgg
     5941 tgaagctcaa ccccgacaat aaggaagcgc actacgggtt gggacatatc tattcctcgc
     6001 aggggcggtt caagctggcc gaggaatcgt tccgtgaggc gattcgtatc gacggtgact
     6061 atgccgaggc gaacacctat ctcgggcagg tgctggccaa tcaggatcga tggaaagaag
     6121 ccatcgcggc ctatcgacag gcgctcagca atccgctcta tccgacaccg gatctggcac
     6181 gcttccactt gggaaaagcc ctgatgcacg aaggcgatct acagggggcc atggaagtgt
     6241 tggaagacgc gacgaccgtc acgccgccga acgtccctcc cgccatgctt caattggagt
     6301 tggcgcgcgt gtatcacaaa ctcggattcg atgtgcgggc gcgtgaggct ctcgcaaagg
     6361 tctcaaactt agacaagggc ggagaacagg cagccgcagc gcaagaactt ctcggtcagc
     6421 tcaaaccata ggcggatatc atggaatcag tgggtgaatt ttttcggcag gtgcgtgaga
     6481 cgaaaggcct gacggtcgat gaggtggcct cgaagaccag gattcgcaca gatttcgtca
     6541 aagcgctcga agagggaaat ttcgccaagt tgcccgacca agtcttcgcg cgcggctttg
     6601 tgcgctccta tgcgcgatcg ttggggctcg acgaagaaga tgccatccat cggtttgtgc
     6661 agtccgccgg tgcgttctat gaaaaacagg gtgagcgaga gcggttgagg cagcggcagg
     6721 tcgaagagga acggcgccgg aaagccaatc gcaaagcggt gggtatcgcc attgctgtcg
     6781 ccattgcaac gctggtgttt ctgctaagtc gggaacagtc ttcgacattg gtgcggcgtt
     6841 ccggatcgga ccagccgccg acgagtaaga agagcgcgcc ttttgctaag gatacccgcg
     6901 aggcgccgcg tcagggcttg gaacttccgc ctcccatcgt gccaccggcg acgaaaccgg
     6961 ctgaacccgt ggccgttccc tcaaagcccg cgcaggaaaa ggtggctgcc gctgtgccgc
     7021 ctcctcccgc tcctccggtc aagccggcgc cggttcagcc ggagcagatg gccgctcaac
     7081 cctccttctc cggatccgat ggtcctctcg cagggctctc tgttgatgga cccgctgggc
     7141 ctgatggtcc gctcgtgctg gatcttgatg ccactgagtt gagttgggtg gtcgtgcagg
     7201 ttgattccgg cagtccccag gaggcgttgc tgcgccctgg tcaaaaggct caatggaagg
     7261 ctcaggacca gtttacggtg acgcttggca atgccggagg cgtccgcgcg gaactcaacg
     7321 gtaagccgca aaagccattt gggccgagcg gaaaggttgt gcgcgatgtc gtcctcaaac
     7381 gttcaggttt gtgatcgggt agtgttctgg tacttgcggc cggtttgagt gttcgtgtcc
     7441 actcacctgc ggttcactct ccataatcgg tgtcgccggg cggctcctcc ggttccattt
     7501 gcacatacga atggggcgcg cagggaatct ctggggcgca aaggtgtcac ggttgaatcg
     7561 tggtacgtgc ctgcccgtga cctgaacatt cagaatcgcc acaatttcag cctctttccc
     7621 cacttctcat cctggcacgc cccttgagtt ccacgtaggt cggcataggc gaagagcggg
     7681 caaggaggct tccagttccc ttgacaagac agaaaacgcg ttgctatagt tcgcctgttt
     7741 tgtcgtcctt gtcacaggct tttttcatgc tgcccaggat aagcgggtcg ttgttcacgc
     7801 ggtgggttca acacaaagga ggagttctga tggggtattt ttctaagtta gttggagtgt
     7861 gtgcggccgt caccttgttg tctgtggcgg tggtgggagc cgaggaaaag gatccgttga
     7921 agccccgtgt tgcaccggat cagatggcgg atgcgaaagc gatgaagaac ccggttgcct
     7981 caaccccgga aagcattgcc aagggcaagg cactgtatga gggcaaaggc acctgcttca
     8041 attgccatgg gaaagaagga aagggcgacg gccctgccgg cgcgatcctc aacccgagcc
     8101 cgcgtaactt caccaactgc aagttccaca agaagcggaa agacggcgag ctgttctggg
     8161 tgatcaagaa tggcagcgca ggcaccggta tggtgtccct gatccctgcg gccatcaacg
     8221 aagaagaagc ctggatgatc atcaactatg agcgcagctt ctgcaagggt ggagaagagt
     8281 agtcgaacgt ttcggccgat cggctgaatc aatggggggc gtcgagggta tcctcgacgc
     8341 cccctttctt ttatgtggga agtgcccgtt tgcgaatcca tgcagagagc gggcgaggag
     8401 ggacggcgcc actccctcct ggatgtagct actgtatgta gcgataggtt gttggtattc
     8461 gggcgaggca gttggcagga attgaatggt gcttcagggt ttgtcggtcg cgggtatcgt
     8521 aggtggtctt gtaagtaccg cgtccat
//
LOCUS       DAOQRM010000104         8486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_7882_length_8486_cov_14.377179, whole genome shotgun sequence.
ACCESSION   DAOQRM010000104 DAOQRM010000000
VERSION     DAOQRM010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7882_length_8486_cov_14.377179"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..125)
                     /locus_tag="PKK81_09020"
     CDS             complement(3..125)
                     /locus_tag="PKK81_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33059.1"
                     /translation="MSWENWAVAIGLGALLMMFFVLMELFFAGPRQPLDHVRRK"
     gene            complement(179..1123)
                     /locus_tag="PKK81_09025"
     CDS             complement(179..1123)
                     /locus_tag="PKK81_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33060.1"
                     /translation="MGRKYDLVDVVVAVGLVATLFGGYLLVTAADGFWQAPVAPAANM
                     TIVTHDPSTGMQYLQPVLGQAILHDVLLDREAGAALSASAMELNRAVSESQLISTTLL
                     SPLVLAELRAFGQEADHRARMQYVMGKSVVNQTRRGLASGVLSADQYAGEFNTNLIQR
                     AESIGQRMHDQFESTRQATLGRSIVEAIQEEDGMAAAVQERIGRAVIQVTQAQEGYAE
                     AKAAGQIQLASAMSAAVRTDALVDRLALLKEMPAGRTIVAYHSTATADISRGALVLAC
                     LGLIALFVGGLAFSSKREVDSIPLWKLETLLQLHRSGR"
     gene            2124..3758
                     /gene="lnt"
                     /locus_tag="PKK81_09030"
     CDS             2124..3758
                     /gene="lnt"
                     /locus_tag="PKK81_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNV33061.1"
                     /translation="MTISRARLILLACASGLLYPLSFPAFDLGIVAWFALVPLHIAVE
                     YLSPGRAFRIGWLSGTLAFTGSMFWVITAMNVYGKVPFPIATLVMLLLTVYLGLYMAV
                     YAGGLSWIRTAFPTLGFLPAPFLWVTLELIRTYFLSGLPWALFGYSQYQWLPIIQLAD
                     HFGVYGVSFLLVLVNAALAETIIWGFKAYRGFQIRSFPWPSSVAATAGFGVALFYGTT
                     LLSSAQHVPTRTLSVGVVQPNVEQAQKWDVAFRQETMARYDRLTAGLGDNLDLIVWPE
                     AATPFVFEMEPQYRAVVNDMARRAGAPLLFGSPALRRHPDGRPFLLNSAYLLATDGQI
                     LGRYDKQHLVPFGEYIPFHNSFLFFLDKLVEGIGDFEAGPGSTLLMFKPREKNPPAVT
                     AATLTDFHVKFGVVICYEVIFPNLVRQFAANGADFMVTVTNDAWFGPSSAPYQHFGMV
                     VFRAVENHVAFARAANTGISGFIDPYGRVVQQSPIFTQEALRGTIAVSHQQTFYSQYG
                     DLFAYACAILIALLCLTGFFSHDTEPAGGEPAGRPA"
     gene            3777..4920
                     /gene="prfB"
                     /locus_tag="PKK81_09035"
     CDS             join(3777..3845,3847..4920)
                     /gene="prfB"
                     /locus_tag="PKK81_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNV33062.1"
                     /translation="MLDDVRTRVRTLGEQLAQLRGHLDLARMTAELNDIEVKTSQPDF
                     WKDTQAAAKVNRRKAALDRELSRWRETERRQNDIEALLELALESGDAGLEQELLAELE
                     PFEKAVAQFRIELLLSGELDSSNAIFAIHPGAGGTESQDWAQMLLRMYVRWAEQKGFK
                     VETLDLLAGDEAGIKSATLSVSGPYAYGYLKAEAGVHRLVRISPFDANKRRHTSFASV
                     FVYPELDDDVQVVIDDKDLRVDTFRAGGAGGQNVNKVETAIRITHIPTGIVVQCQNER
                     SQLQNRNGAMKILKARLFEVEQRKKEAEFNAIVGEKKDIAWGSQIRSYVFQPYQMVKD
                     HRTGYEVGQVSAVMDGELDGFIEAYLQRKLTGGKAPEPVAMKDDDL"
     gene            5022..6503
                     /gene="lysS"
                     /locus_tag="PKK81_09040"
     CDS             5022..6503
                     /gene="lysS"
                     /locus_tag="PKK81_09040"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNV33063.1"
                     /translation="MDELNDQRQQRIKKLDQLRQLGVAPYGTRFEVKDRAGQLTRLHG
                     EKTKDVLEQEQISCTIAGRIVGLRRFGKAAFAVLQDGADRLQVYLKKDTLGEQAHQIS
                     EGLDLGDWIGVTGVLFRTKTNEFTVEVRTLTFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPQVHQIFALRSRIVSGIRAFLIERGFLEVETPMMHPIPGGAAAKPFVTHHNA
                     LGAELYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVSYADY
                     HDLIVLTEELFGRLAQDILGTTTIDYQGTQINLGSPWRRWSYHQSILEVNSLPTSVLT
                     NRDEALAAAQRLGVEVSPKESLVNILNEIFEETVEPRLQQPTFITDYPIEISPLARRK
                     DSDPSLTDRFELYIAGREIANAFSELNDPLDQRERFEAQAAKHAAGDEEAHRVDEDFL
                     RALEYGMPPTAGEGIGIDRLVMLFTNQSSIRDVVLFPQLRPEK"
     assembly_gap    6667..6766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6767..7635
                     /locus_tag="PKK81_09045"
     CDS             <6767..7635
                     /locus_tag="PKK81_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNV33064.1"
                     /translation="NLDDLARQNKVVIPEKEREPNGSDTAMRPGIILGKELSMRLGAF
                     PGDTLNVVSPVGPVTGASMTPKIRQFVVVGIFQSGMYEYDSSLAYIELGEAQKFFNMG
                     ASVTGIEVKVTDVFRAADIARAIEHHLGFAFWARDWMQMNRNLFSALKLEKTMMFLLL
                     VLITIVASFNIVSTLTMIVTEKQREIAILKAMGATRKGIMRIFMLNGLIIGCSGAAIG
                     VPLGYTFLWLIQTFWTFDPTVYYISRIPVHVLGSDVLLVAGSAILISFVATLYPSLQA
                     AKLDPAAALRYE"
     gene            7698..8366
                     /locus_tag="PKK81_09050"
     CDS             7698..8366
                     /locus_tag="PKK81_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV33065.1"
                     /translation="MIDVVDLYKSFPMGGRELVVLNNINLHIKRGELIAIMGASGAGK
                     STLLQILGTLDRPTKGTVSFDGQNLFTLTEQEQAEFRNKRVGFVFQFHHLLPEFSALE
                     NACLPAMIQKRDMADVVGEATKLLGEVGLADRLHHKPGELSGGEQQRVSVARALMQQP
                     DLVLADEPTGNLDSHTGDALFTLLRQLNRSRGTTFVIVTHNDKLSSQADRIVSMQDGM
                     IVSS"
BASE COUNT         1777 a         2635 c         2283 g         1691 t
ORIGIN      
        1 cctcattttc ttcgcacatg atccagtggc tgccgcggac cggcaaagaa caactccatc
       61 aacacaaaaa acatcatgag gagtgcgccc agcccgatgg cgacggccca gttttcccac
      121 gacatgtctg acctcctctt gcccttgaaa atcgtcgaac gattcccagg gggcagcgtt
      181 acctgcctga acggtgcaac tgcaacaacg tctccagctt ccacagcgga atactgtcca
      241 cctccctctt gctgctgaac gccaaaccgc ccacgaacaa tgcaatcagc cccagacagg
      301 ccagcaccaa ggccccgcgc gaaatatccg cagtggcagt cgagtggtat gcgactatcg
      361 tgcggccggc cggcatctcc ttgagcagag ccaaacgatc caccaatgca tccgtgcgaa
      421 cggccgcact catcgcactg gcgagttgaa tctgcccggc cgccttcgcc tccgcatacc
      481 cttcttgcgc ctgcgtcacc tgaatgacgg cccgcccgat tcgctcctgg accgccgccg
      541 ccataccatc ctcctcctga atcgcctcga ctatcgagcg gccaagtgtc gcctgcctgg
      601 tcgattcaaa ctgatcgtgc atgcgctgcc cgatcgattc ggcccgctga atcaggttcg
      661 tgttgaattc gccggcatac tgatcggcag agaggactcc gcttgcaaga ccgcggcgag
      721 tctggttgac gacggacttg cccatgacat actgcatgcg cgcacgatga tcggcttcct
      781 gtccaaacgc gcgcaactct gccaacacca gcggactcag caacgtcgtc gagatcagct
      841 gagactcact caccgcccgg tttaactcca tggctgaagc agagagggcg gcaccggcct
      901 cccggtcgag caagacatcg tgaagaatcg cctggcctaa aaccggctgc agatactgca
      961 ttccggtgga ggggtcgtga gtcacgatgg tcatattggc agcgggagcg accggcgcct
     1021 gccagaaacc atcggcagcc gtcaccaata ggtacccgcc gaacagcgtc gccaccaatc
     1081 cgacggcgac caccacatca acgaggtcat attttcgtcc catgatatcc tcctatcctg
     1141 cctcgggtag ttcggcgcgt ggcaaacagg caggacgccg gcgcgccgga atctgagccg
     1201 caccagatcg ctgtcgttta agcatagaga cctcttcctt ggtgtgatgg agcacttgca
     1261 gaatggaaat atattcccgg ccttgcgagg cacaaagcgc gggctgcaga tcttgatgcg
     1321 gaacgtcgtt gatgaaggat accggccgga ggagacggga caaggctggc ttggtcagag
     1381 cggcggtcat gttcgccacg gaagaccatg cgaccacctg cagaaagggt ggcattgaat
     1441 gacttagtta gtctatgcct cgctcaaaaa gcagtcaata cggcaagaac taagagccgg
     1501 cgcgggttgc cgggcatgtg ctctcccgca tcattcaaca tttgaaaaac gtgattcacc
     1561 tctcaggaat agacatgcag tacaatgtgg aaagagatcc ctatgcgtat cttcgagagg
     1621 gagtgtccta tgccaaccac tagcctgctg atgctactta ccgcatggac gctattaggc
     1681 ccggtttcca tcgctggctc ccagccatcg atccttcaac agcgggacgg gaccaccagc
     1741 cccctgactc cgctgggtga gcaagacgcc atctactccg acgcgcatgg aaacaaaggt
     1801 gtcacccatc tggggtccgg ccttccgtca cacacgttca gttctcccca tggcgcagtg
     1861 cccggatcag tcacaccatt cggaacaccg acaccgccga atctcgtcac accggcgccg
     1921 ttactgccgc tccaaccaag agatatggcg actccatacc ctcagccccc cgcatcatcc
     1981 gatagtccag gcggatacag caggttcggc gggcgatcgg ggcgctagcc ggtcgaacga
     2041 ccagtctggg atgatcctcc cccaagccca tccgccaaag agcacggttg aatctcttca
     2101 ggtctttcgt taagttacag gcaatgacca tctcccgcgc acgcctcatt ctgcttgcct
     2161 gcgcgagcgg tcttctctat cccctctcct tccccgcgtt cgacctcggc atagtggcct
     2221 ggttcgcgct ggtcccgctg catatcgcgg tcgaatacct gtcgccgggt cgcgcgttcc
     2281 gtatcggctg gctttccggc acgctggcct tcacgggctc gatgttttgg gtcattaccg
     2341 ccatgaacgt ctacggtaag gtcccgttcc cgattgccac cctggtgatg ttgttgctca
     2401 cggtctacct cggactctac atggccgtct acgcaggcgg actttcgtgg atccggacag
     2461 cgttcccgac cctgggcttc ctacccgcgc cttttctctg ggtgacattg gaactgatcc
     2521 gtacctactt tctctccggc ttaccctggg cactattcgg atattcccaa taccaatggc
     2581 tcccgatcat tcagctggcc gaccatttcg gggtgtacgg cgtctccttc ctcctcgtgt
     2641 tggtcaacgc tgcgctggct gaaacaatta tctggggctt caaggcctat cgcggatttc
     2701 agattcgttc gtttccgtgg ccctcgtcgg tggcggctac ggccggattc ggcgtcgccc
     2761 tgttttatgg caccaccctc ttgtcgtccg cccagcatgt cccaacccgc acgttgtcgg
     2821 tgggagtcgt acaaccgaat gtggaacagg cacagaaatg ggatgtggca tttcgccagg
     2881 agacgatggc acgctacgat cggctcacgg cgggactggg cgacaatctg gatctcatcg
     2941 tctggccgga agccgccaca ccctttgtat ttgaaatgga accgcagtac cgcgcagttg
     3001 tgaacgacat ggctcgccgc gccggtgccc cgctgctatt cggcagcccg gctttgcgac
     3061 gtcatccgga cggtcgcccg ttcttgctca acagcgcgta cctgctcgcc accgacggcc
     3121 agatcctcgg tcgctacgac aagcaacatc tggtcccctt cggcgaatac attccgtttc
     3181 acaattcatt cttgttcttc ctggacaaac tcgtcgaagg catcggcgac ttcgaggccg
     3241 gaccgggatc gacgttattg atgttcaagc cccgcgagaa gaacccgcct gcggtgaccg
     3301 cagccaccct caccgatttc cacgtgaagt tcggcgtcgt catttgttac gaagtgatct
     3361 tccccaacct cgtgcggcag tttgcggcga acggagccga tttcatggtc acggtcacga
     3421 acgatgcctg gttcggtccg tcgtcggcgc cctatcaaca ttttggcatg gtagtctttc
     3481 gtgccgtgga aaatcatgtc gccttcgcgc gtgcggccaa caccggcatc tccggattca
     3541 tcgaccccta cgggcgtgtc gtgcaacagt caccgatctt cacgcaggaa gcgctcagag
     3601 gaacgattgc cgtcagccat cagcagacct tttactcgca atacggtgat ctctttgcgt
     3661 atgcctgtgc tatactcatc gcgcttttgt gcctgaccgg cttcttctca cacgacacag
     3721 aacccgccgg tggtgaacca gccgggcgac cagcctgagc gagaaaggac cgtcccatgt
     3781 tagatgatgt ccgaacccgt gtgcgtaccc tcggcgaaca gttggctcaa ctacgggggc
     3841 atctttgacc tggctcggat gaccgctgaa ctcaacgaca tcgaagtcaa aacctcccag
     3901 ccggatttct ggaaagatac gcaggcggcc gccaaagtga accgccgcaa ggccgccctc
     3961 gatcgggaac tatcccgctg gcgggagacc gaacgccggc agaacgacat cgaggcactc
     4021 ctcgaactgg cgctggaatc cggcgatgcc gggctggagc aggaactcct ggccgaactc
     4081 gaaccattcg aaaaagccgt cgcccaattc cgcatcgagt tgctgctctc aggcgaattg
     4141 gactcgagca acgcgatttt cgccatccac cccggggccg gcggaaccga gtcacaggac
     4201 tgggcgcaga tgctgctgcg catgtatgtg cgatgggccg agcaaaaggg atttaaggta
     4261 gagacgttgg atctgctcgc gggggacgag gccggcatta aaagcgcaac gctgtccgtc
     4321 agcggaccct atgcctacgg ctatctcaaa gccgaagccg gcgtgcatcg ccttgtgcgg
     4381 atctccccgt tcgacgccaa caaacgacgg catacgtcct tcgcgtcggt gtttgtctac
     4441 cccgaactcg acgacgatgt gcaagtggtg atcgacgaca aagacctgcg ggtcgatacg
     4501 tttcgagccg gtggcgccgg cggtcagaat gtgaacaagg tcgaaaccgc catccgtatc
     4561 acccacattc cgaccggcat tgtggtgcaa tgtcagaacg aacggtctca attgcaaaat
     4621 cgcaacggcg ccatgaaaat tttgaaggcg cgcctctttg aagtggagca gcggaagaag
     4681 gaagccgagt tcaatgccat cgtcggtgaa aagaaagaca tcgcctgggg cagccagatc
     4741 cggtcctatg tgttccaacc ctaccagatg gtcaaagacc atcggaccgg atacgaagtc
     4801 ggccaggtct ccgctgtcat ggatggtgag ctggacggtt tcatcgaagc ctatctccaa
     4861 agaaaactga ccggcgggaa agccccggag ccggtcgcca tgaaggatga tgacctgtaa
     4921 agaaagggag ctgatagcct atggcctata gcagatagca gaaaacggaa cctgttccgg
     4981 accatcagct attcgccagt agccatacgc tccgtgtgat catggatgaa ctgaacgatc
     5041 aacgccaaca acgcatcaag aaactcgacc aacttcggca actcggggtg gctccatatg
     5101 gcacgcgctt tgaggtgaag gaccgggccg gacaactcac gcgtctgcac ggtgaaaaaa
     5161 ccaaggacgt gctcgagcag gagcagatca gctgcaccat cgccggccgc atcgtcggac
     5221 tacgccggtt cggtaaggcc gccttcgcag tactgcaaga tggggcggac cgcctgcagg
     5281 tgtatctgaa aaaggacaca ttgggcgagc aggctcacca gatttccgaa ggactggatc
     5341 tcggcgactg gatcggagtc acgggtgtct tgttccgcac caagaccaac gaatttaccg
     5401 tcgaagtgag gaccctcacg tttctgagca aagcgctacg cccgttaccg gaaaagtggc
     5461 atgggctcac cgatgtcgaa acccggtatc ggcagcgcta cgtcgatctc attgccaatc
     5521 cccaggtaca ccagatattc gcgctgcgca gccgcatcgt gtccgggatc cgcgcgtttc
     5581 tgatcgaacg cggctttctc gaagtcgaaa cgccgatgat gcacccgatt cccggcggcg
     5641 ccgccgcaaa acccttcgtc acccatcaca atgccctggg cgccgagctc tatcttcgca
     5701 ttgcaccgga actctatctc aagcggctga tcgtcggcgg cttcccgcgc gtgttcgaga
     5761 tcaaccggaa tttccggaac gaaggcatct cgacgattca caatcctgaa ttcaccatgt
     5821 tggagtttta cgtctcctac gccgactacc acgacttgat cgtgctgacc gaagaactct
     5881 tcggccggtt ggcccaggac attctcggca ccaccaccat cgactatcaa ggcacacaga
     5941 tcaatctggg atcgccgtgg cgccgatggt cgtatcacca atcgattctg gaagtgaata
     6001 gcctgccgac ctccgtcctc acgaatcgcg atgaggccct tgcggcggcc caacgcctcg
     6061 gtgtcgaagt ctctcccaaa gaatcactgg tgaatatcct caacgagatc ttcgaagaga
     6121 ccgtcgagcc gcgcctccaa cagccgacct tcatcaccga ctacccgatc gaaatttccc
     6181 cgctcgcgcg aaggaaagac tccgatccgt cgctcaccga ccggttcgag ctctacatcg
     6241 cggggcgtga aatcgccaac gccttctccg agttgaacga tccgctcgat cagcgcgagc
     6301 gcttcgaggc tcaggcggcg aaacatgcgg ccggagacga ggaagcccac cgcgtcgatg
     6361 aagacttcct gcgcgccctc gaatacggca tgccgcctac tgcgggcgaa gggatcggca
     6421 tcgatcggct ggtcatgctc ttcaccaatc agtcctcgat ccgggatgtg gtcctcttcc
     6481 cgcagctgag gcctgaaaag tagacatggc gctgccctac gaaatcttca tcggattgcg
     6541 gtacctgcgc gcgaagcgac gcaaccgcac catctccttc aatacgatcg tctcgatcgc
     6601 cgggattacg ctcggcgtcg ccgccttgat cggcacggtc gggatcatga ccgggttcaa
     6661 ggaggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcaa cctcgacgac
     6781 ctcgcgcgtc agaataaagt cgtcattccc gaaaaagaac gggagccgaa cggttccgac
     6841 acagccatgc ggccgggaat cattctgggg aaagaactct caatgcgact cggcgccttc
     6901 cccggcgaca ccctgaatgt ggtctccccc gtcggcccgg tcacgggcgc cagcatgacg
     6961 ccgaaaatcc ggcaattcgt cgtcgtcggc atctttcaat ccggcatgta cgaatacgac
     7021 tcctccctcg cctacatcga actgggagag gcgcagaagt ttttcaacat gggcgccagc
     7081 gtgacgggta tcgaagtcaa agtgaccgat gtgttccgcg cggcggacat tgcccgcgcg
     7141 atcgagcacc atctcggatt cgccttctgg gccagagact ggatgcagat gaaccgcaat
     7201 ctgttttcgg cgctcaaact ggaaaagacg atgatgttcc tcctgctggt cttgatcacc
     7261 atcgtggcct ccttcaatat cgtcagtacc ctcaccatga tcgtgacgga aaagcagcgc
     7321 gagatcgcga tcctcaaagc gatgggcgcg acgcgtaagg gcatcatgcg tattttcatg
     7381 ctcaatggtc tgatcatcgg ctgttccggt gccgccatcg gcgtcccgct ggggtataca
     7441 tttctgtggc tgatccagac tttttggacc ttcgacccga ccgtgtacta catctcccgc
     7501 attccggtcc atgtgctcgg ttcagatgtg ttgctggttg ccggttcggc gattctgatc
     7561 agcttcgtcg ccacgctcta tccgtcactc caggcggcca aactcgatcc ggcggcggcc
     7621 ttgcgctatg aataagagag tcggggcatt ccgggcagta cgattgaacg tgaaccatta
     7681 cagatcgcag caccgacatg attgacgtcg tcgatctcta caaatcgttt ccgatgggcg
     7741 ggcgggaact cgtcgtgttg aataacatca acctgcacat caaacgcggg gaattgatcg
     7801 cgatcatggg cgcctccgga gccggcaaga gcaccctgct gcaaatcctc ggaaccctgg
     7861 atcgcccgac caaaggcacc gtgtcgttcg acggccagaa tctttttacg ctcaccgagc
     7921 aagagcaggc ggaattccgc aacaaacgtg tcgggttcgt gtttcaattc caccatctgt
     7981 tgccggaatt ttccgccctc gaaaatgcct gtctcccggc gatgattcaa aaacgcgata
     8041 tggccgatgt cgtcggcgaa gcgacgaaac ttctcggcga ggtcggtctg gccgatcgac
     8101 tccaccacaa accgggcgaa ctctcaggcg gcgagcaaca acgtgtctcc gtcgcgcgcg
     8161 ccttgatgca gcagcccgat ctggtgctgg ccgatgaacc gacaggcaac cttgactctc
     8221 acaccggcga cgccctcttc accttgctcc gtcaattgaa ccgctcacgg ggaactacgt
     8281 tcgtcatcgt cacccacaac gacaagctct catcgcaggc cgatcgtatc gtctccatgc
     8341 aggacgggat gatcgtgtcc tcataggccg ataccatcag cgatggcccg cgccggctca
     8401 cgcggcacgg ctgagtcgtc cgaccttgat tgggatttct tgacggcgtc agcatctttt
     8461 cgtagactga cccatcctcg cattca
//
LOCUS       DAOQRM010000105         8423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_7975_length_8423_cov_12.395196, whole genome shotgun sequence.
ACCESSION   DAOQRM010000105 DAOQRM010000000
VERSION     DAOQRM010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7975_length_8423_cov_12.395196"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            989..2524
                     /locus_tag="PKK81_09055"
     CDS             989..2524
                     /locus_tag="PKK81_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV33066.1"
                     /translation="MAKEPLQVLVLVGAMVMISGCMIKPYVLTDEDVRTRATNDLQMV
                     STLREPLSGPIDLYEAIARALKFNLDAKVKAMQAQLAHQQLNVAHYTLLPQLSANAGF
                     DGRNSYSGGGAQSLVDGRPVLEPFTSSDKNVVSGNLALSWDVLDFGLSYVRAQQAADN
                     VMIAEEERRRIAVRLVQDVRSAYWRAVSAERVLYRVKFLDESVNKALERAQQIVDKKL
                     QTPLTPLNYRRDLLNIQREVQRLYRELSTARVQLASMMGLPPGTEYDLVVPQRASTVP
                     VVDLDTGRMEEQALLLRAELRSLDYQKRINAKEARAVFLEMFPNLKLSLGGYYSSNSF
                     FLYQNWLGYASQLSWNLLSAFRVPAKLKAVEAHRQVLDAQSLALTMTILTEVHVGALQ
                     FAQASQEYLNARSYHETQLAITDHTKNLWLTKSTNDLTFIRERVNDVLAEVRMDSAQS
                     GVESAYATLMASMGEDAAPTGTDGQSVAALADELRKQRVPAVDTVSESGRRLESHAIP
                     LAN"
     gene            2502..3287
                     /locus_tag="PKK81_09060"
     CDS             2502..3287
                     /locus_tag="PKK81_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV33067.1"
                     /translation="MRSHWLINSMVALTIFCAAVAFPQVGWPKDDRPVQLMRSVNGAL
                     RGIVKASTQAVLYSQIQGRISQIPVKEGQRFEKGATLVAIDCDKYRAELAGALAEHEA
                     KDKTFQNNKELTKLNAVSTLELEISEAEAKKAVAAIRLAEINVRSCHIAAPFSGRVVA
                     VMVNEHENVFPNDKLLSLLDDTSLEIELVLPSSTLAWLKRKSPFTFTIDETHREYPAK
                     VKEIGANVDPASQTVKVVGSFGALPADVLAGMSGTAQFASGQP"
     gene            3292..4656
                     /locus_tag="PKK81_09065"
     CDS             3292..4656
                     /locus_tag="PKK81_09065"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNV33068.1"
                     /translation="MATSTQPSAQELATLVHLATLTHLEAQIRVAKTVQELQFISVNE
                     TRRLVPYEQAYLLSHAGEAAGVSRVICASSVAVVERDAPMMRWLEEMAQALRHALPGD
                     EPVTVTEEMCPDSCREGWREHVRGHVLWCPLKHPDETVLGFWWLERDYPWEENDRAIM
                     HRLAGSFAYAWKAVATRHRTWSVRIKTPSVWLMAAAVVGALCLPVRVSTVAPVKVMAK
                     EPTIVSAPMDGVIAEVLVLPNTVVTQHRPLFRYEDTNLRNQFRVAEKQLAVAQAEHAQ
                     AIQGGFADPQRKAEVPLRAAEAALKETELTYAQEMLAQVEVRAPQEGLLLYSDKADWV
                     GKPVSVGERIMDIADPRRIELRVDLPVSDALLLREGADVVAFFDALPLESFGATVSRA
                     SYHAEVLLGDMLAYRITAELATADPRIRIGWQGSAKVYGEQGPLAFLLFRRPLMALRQ
                     LVGV"
     gene            4660..>6387
                     /locus_tag="PKK81_09070"
     CDS             4660..>6387
                     /locus_tag="PKK81_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNV33069.1"
                     /translation="MAGLSSVSPPQERTLPPLRKNLQFLRGAPTPEGVPTWTIVDPVR
                     NKYFQIEWQVYQILQRWSCGTVEKLVAVVRRDTTSRIRAEDVEDLVRFLYANSLTEQS
                     ASGRVKDYVEQEVARHQAWWQWLLHHYLFIKIPLIHPHRFLHATLPLVAPLYTPMAAW
                     LFGMIGATGLFLVGQQWDTFLSTFLHFFTWSGALMYGAVFCAVKVVHELGHAYTATRF
                     GCRVPTIGVALMVMMPVLYSDISDAYRLSSRRKRLWIAGAGVVAELGLAAVATLVWGF
                     LPDGAIRSVVFVAATTSWVMSLAVNLNPFMRFDGYYLLADGLGIPNLQDRAFAFGQWR
                     IRELLFAPGAPPPEAVGLSNRRILIAYAWGIWLYRLVLFTGIALAVYHYFFKVLGLLL
                     FLVEIVFFIGLPIWRELTSWWSRRAAYASTGRFAVTMTVVAAMLLLACLPWSSRVAIP
                     GVLQAGSYATIYPPAPGRIASVSVHAGQSVRAGDTVLVLENPLLAKEARLARTQVELL
                     DFRLQRQAGYAEDRLQGQVLAESLRSKLVELDGVAEKQQRLVLQAPIDGVVVDQADSL
                     YPGRWINEKL"
     assembly_gap    6388..6487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6488..>8423
                     /locus_tag="PKK81_09075"
     CDS             <6488..>8423
                     /locus_tag="PKK81_09075"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HNV33070.1"
                     /translation="TEVVFVDPTVPDYESLLAGMGPNVEVVVLDASRDGVEQIAESLT
                     GRSGIDAIHLISHGDAGTLQVGTGTLTAESMSTRYVDELATVQQALSEQADILVYGCN
                     FAEGDVGQAAVARLAELTGADVAANSDRTGHIDLGGDWKFETQVGSIETDLAITDAAQ
                     MDWKGILTTETVGDQFSLSSYDNHDGTQKWASDWSETDAGGGGASGGDVHVASSQLRI
                     DAGSVGNQASRQVDLSGTSGAILTFDYTNSLSGSDQIEIRVSADGKTYTTLTDGVFSP
                     KSNAGSGTVKLDISGYASASTTIQFLVTGVGGGDSLSVDNVQVSYDTGAANSAPTISS
                     NGGGTTASVTVGENSTVVTTVTATDVDAGHTLGYSIAGGADAGRFTINNSTGQLSFAS
                     APSYEAPADSGGNNVYDVTVQVSDGQGGTDTQALAVTVANVNEAPTDLALSANTVAEN
                     AANGTVVGTVSGTDSDTGDTRTYSLTDNAGGRFAINSSTGQITVANRSLLNYESVTSH
                     NLTVRVTDSGGLTYDETFTINVTDVNEAAPRITSNGGGATALINIAENATAVTRVTAS
                     DADTRQTLAYSIVGGADAAKFTINSSTGALSFVSAPNYEAPTDSGGNNVYDVTVQVSD
                     GSGGVDSQAISVTVTAVNERP"
BASE COUNT         1589 a         2359 c         2674 g         1701 t
ORIGIN      
        1 cgcgctgctt cagtgccctg gcccggccgc tgttgttggc gagtcgactc ctggcttgga
       61 gtcgagccaa tcagaccgtt cggggtctct ggctgtcgtg ctcgctgtct ggacgattga
      121 atggggaaca tgatcgcatg gttctctccg gtctattaat cgcgaggcaa cattccagaa
      181 tcgttggttg gcggcgtcag gctgtcggtg taggcggcct gatgttccct taccgcttca
      241 agcctgtaga gtgctccgtc agcaacgcca tctttggccg gcactgatcc ctctcctagc
      301 gggttattct aagagttgcc ccagcatgtg ccccgcttgt ctgattcaat aggtaggcca
      361 ttcccctgcg tcgggagacg tggtcgcgat cgtcacttct gttctcagag ggcaccttcg
      421 tcagtgtgcc ttcacccggt agccgttctc gttgcgggcg tagattaagt ctggcctcgg
      481 tgaggcttgc cacttctgag gctactgact gggattgcgg ctgaagcgcg gaggctcagg
      541 aaaaggttga aatacggaac gaccgggatt gcaacttgct cctgtcgata tcctgcgctg
      601 aggcaccgaa cttcgaagtt cgcgatgtcg gttttctatt gcctgtgcgc ttcatgatca
      661 cactgtgcgc cttcccagga tcgatgccct ccgggctgcg atgcgcaggc gacgaacggt
      721 gaggtcttgc cagggcgtgg ctccgctcga ttcgctctcc acccgcagcc gttccataga
      781 tttgttgagg cggtgtcgtc tggctcgcct tagtacggac gacttgcagt gcgccatcta
      841 gcggtcgcca tcggcatccc gcttcatcct ctttcgtcaa gttcttgtcg aattcgaccg
      901 atattcctgc agtaagggtg tgtcgtggcc tgggatggat ccgtgcgcgc acgatagtga
      961 tgacggtgtc tgcggaatca tgaagacgat ggcaaaagaa cccctgcaag tattggtatt
     1021 ggtgggggca atggtgatga tctccgggtg catgatcaag ccctatgtgt tgaccgacga
     1081 ggatgtccgg acccgcgcca ccaatgacct tcagatggtg tcgacgcttc gggagccgct
     1141 gtccggtccg atcgatctct atgaagccat cgcgcgtgcc ttaaaattca atctggacgc
     1201 caaggtgaag gccatgcagg cgcagttggc gcatcagcag ctgaacgtgg cgcattacac
     1261 cttgttgccg cagctgtcgg cgaacgccgg atttgacgga cgcaattcct acagcggagg
     1321 cggcgcgcaa tcactcgttg atggtcggcc cgtcctcgag ccctttacgt cgtccgataa
     1381 gaacgtggtg tcgggtaatc tcgcgctgag ttgggacgtg ctggatttcg gtctttccta
     1441 tgtgcgcgca cagcaggcgg ccgataacgt catgattgcc gaggaggagc ggcggcggat
     1501 tgcggtacgc ctcgtccagg atgtccggag cgcctactgg cgggcggtca gcgcggaacg
     1561 ggtgctctat cgggtgaaat ttctggatga gtccgtcaac aaggcgttgg agcgcgcgca
     1621 gcaaattgtc gataagaaat tgcagacgcc cttgaccccg ctcaactatc gccgggactt
     1681 gctgaacatt cagcgtgagg tgcagcgcct gtaccgtgag ctgagcacgg cacgggttca
     1741 gctggcttca atgatggggc tgcctccggg aaccgagtac gacctggtcg tgcctcagcg
     1801 ggcgagcacc gttccggtgg tcgatctgga tactgggcgg atggaggaac aggcgttgtt
     1861 gcttcgtgcc gagcttcgct ccctcgacta tcaaaagcgg atcaatgcga aggaggcgcg
     1921 ggccgtcttc ctggaaatgt ttcccaactt gaagctgagt ctcggcgggt attacagcag
     1981 caacagcttc ttcctctatc aaaactggct ggggtatgcc tctcagctca gttggaacct
     2041 tctgtcggcg tttcgcgtac cggccaaact caaagcggtg gaggcgcatc gacaggtgtt
     2101 ggatgcccaa agtctcgcgc tgaccatgac gatcttgacc gaggtgcatg tcggcgcgct
     2161 gcagttcgcc caggcgagcc aggaatatct caatgctagg agctaccacg agacgcaatt
     2221 ggccattacc gatcatacca agaacctgtg gctcacgaag agtaccaacg atctgacctt
     2281 cattcgggag cgagtgaatg acgtgctggc tgaagtccgc atggacagcg cgcagtccgg
     2341 cgtcgagtcg gcctatgcca cgttgatggc atcgatgggc gaagatgccg cgccgaccgg
     2401 caccgacggg cagagtgtgg ccgcccttgc cgatgaactg aggaaacagc gggtgcctgc
     2461 cgtcgacacg gtgtcggagt caggaaggag gctggagtcc catgcgatcc cattggctaa
     2521 ttaattcgat ggtcgcactg acgatcttct gcgcggcggt ggcctttccg caggtcggct
     2581 ggccgaaaga cgaccggccc gtccagctga tgcggagtgt caacggcgcg ttgcgtggca
     2641 ttgtgaaggc cagcacgcag gccgtgctct attcccagat tcaggggcgg atcagccaga
     2701 tcccggtgaa ggaggggcag cggttcgaga agggcgcaac gctggtcgca atcgactgcg
     2761 acaagtatcg ggcggaatta gcgggggccc tggccgagca tgaggccaaa gacaaaacct
     2821 tccagaacaa caaagaactc accaaactca atgccgtcag cacgcttgaa ttggaaattt
     2881 ccgaagccga ggcgaagaag gccgtcgcgg ccattcgcct ggccgagatc aacgtgcgaa
     2941 gctgccatat cgcggcgccg ttttccggcc gcgtcgtggc ggtcatggtc aatgagcatg
     3001 aaaacgtctt ccccaacgac aagctgctca gtcttctcga tgacaccagt ctggagatcg
     3061 agttagtgct gccctcctcg accttagcct ggctcaagcg gaagtcaccg ttcacattca
     3121 ccatcgacga gacgcatcgg gagtatccgg cgaaggtgaa ggaaatcggg gcgaatgtcg
     3181 atcccgccag tcagacggtc aaggtggtgg gcagcttcgg ggccctcccg gccgatgtgc
     3241 tcgccgggat gagcggcacg gcgcaatttg catcgggaca accgtaaccc tatggcgaca
     3301 tccacccaac cgagcgcaca ggaactcgcg acgctggtcc atctggcgac cctcacccat
     3361 ttggaggcac agatccgtgt cgcgaagacc gtccaggaat tgcagtttat ctccgtcaat
     3421 gagacccgcc ggctggttcc ctatgaacag gcctatctgc tgagtcatgc cggtgaagcg
     3481 gccggcgtct cccgagtgat ctgtgcgtcc agcgtggccg tggtggaacg cgatgcgccg
     3541 atgatgcgct ggttggaaga gatggctcag gctctgaggc acgcactccc gggggatgaa
     3601 cccgttaccg tcacggagga aatgtgcccc gactcctgcc gcgagggatg gcgggagcat
     3661 gtcaggggcc atgtcctctg gtgtccgctg aaacacccgg atgaaacggt gttgggtttc
     3721 tggtggctcg aacgggacta tccctgggag gagaatgatc gggcgattat gcaccggctt
     3781 gcggggagct tcgcctatgc ctggaaggcc gtggcgacgc gccatcgaac ctggtccgtg
     3841 aggatcaaaa cgccctcggt gtggttgatg gctgcagcgg ttgtcggggc gctgtgtctg
     3901 ccggttcgcg tgtcgaccgt ggcgccggtg aaagtgatgg cgaaggagcc gacgattgtg
     3961 agcgcgccga tggacggagt gattgccgag gtcctggtgc tgccgaatac ggtggtcacg
     4021 caacatcggc cgctgttccg ctacgaagac acgaacctcc gcaatcagtt ccgggtcgcg
     4081 gagaagcaat tggcggtggc gcaggccgaa catgcccagg cgattcaagg cggctttgcc
     4141 gatccgcaac gaaaagccga ggttccgctc agagccgccg aagcggcgtt aaaggagaca
     4201 gagctgacct atgcccagga gatgctggcg caggtggaag tccgtgcccc gcaggaagga
     4261 ttgctgctgt atagcgacaa agccgactgg gtcgggaagc cggtctccgt cggcgaacgg
     4321 atcatggata ttgccgatcc gcgtcgcatt gagttgcgag tcgatctccc tgtgagtgac
     4381 gccctcctgt tacgggaagg cgccgatgtc gtagcgttct tcgatgcact cccgctggaa
     4441 tcgttcgggg cgaccgtgag tcgcgcgagt taccatgcgg aggtgttgct cggagatatg
     4501 ttggcgtatc ggatcacggc tgaattggcg actgccgatc cccgtatccg cattggctgg
     4561 caagggtcgg cgaaggtgta tggagaacag ggcccgttgg cattcttgtt gttccgccgt
     4621 ccgctcatgg ccttgcgcca actggtcgga gtgtagccga tggccggcct gtcctcagtg
     4681 tccccgcccc aagaacgcac cttgcctccg ctgagaaaga acctgcagtt cctccgcggc
     4741 gcgccgacac cggaaggggt gccgacctgg acgatcgtcg atccggttcg caacaaatat
     4801 ttccagatcg agtggcaggt ctatcagatc ctccagcgct ggtcctgtgg cacggtggaa
     4861 aaactcgtcg cggtggtccg gcgcgacacc acctcccgga tccgggctga agacgtcgaa
     4921 gacttagtgc ggttcttgta tgccaacagt ctgacggagc aatcggccag cgggcgggtc
     4981 aaggactacg tcgagcagga ggtggcacgc catcaggcct ggtggcagtg gctgttgcat
     5041 cactatctct tcatcaaaat tccgctgata catccgcatc gttttctgca cgccacactg
     5101 cccttggtgg cgccgctcta cacacccatg gcggcgtggc tgttcggaat gatcggtgcg
     5161 accgggttgt tcctggtggg acagcaatgg gacacctttt tgtcgacctt ccttcatttt
     5221 ttcacttgga gcggggcgct gatgtatggc gcggtcttct gcgccgtcaa ggtggtgcac
     5281 gaattggggc atgcgtatac ggcgacgcga ttcggatgcc gggtacccac gatcggtgta
     5341 gccttgatgg tgatgatgcc ggtgctctat tcggacatca gcgacgccta tcggctgagt
     5401 tcgaggcgga aacgtctgtg gatcgccgga gccggggtcg tggccgaact gggacttgct
     5461 gcggtcgcga ccttggtgtg gggcttcctg ccggatgggg ccatccggag cgtcgtcttc
     5521 gtggcggcga cgaccagctg ggtgatgagc ctggcggtca acctcaaccc atttatgagg
     5581 tttgacggct actatcttct ggccgatgga ctgggaattc cgaatctcca ggaccgggcc
     5641 ttcgccttcg ggcaatggcg gattcgtgaa ctgttgtttg cgccaggcgc gcctccgccc
     5701 gaagcggtcg ggctgtccaa caggcggatc ctgatcgctt atgcctgggg tatctggctg
     5761 tatcggcttg tcctgttcac cgggattgcg ctggccgtct atcactattt ttttaaagtg
     5821 ctcggcctcc tgctgtttct ggtcgaaatc gtcttcttta tcggtctgcc gatctggcgg
     5881 gaactgacga gctggtggag tcgccgtgct gcctatgcat cgacgggccg gtttgccgtc
     5941 acgatgacgg tcgtcgccgc gatgctgttg cttgcctgtc ttccatggtc cagccgtgtc
     6001 gcgatcccag gggtgctgca ggcagggtcc tatgcaacca tctatcctcc ggcgccgggg
     6061 cggattgcat cggtgtcggt gcatgcaggg cagtccgtac gagcgggtga caccgtgctc
     6121 gtactcgaaa acccgctatt agccaaggag gcccggttgg cccggaccca ggtcgagctc
     6181 ttggatttca ggctgcaacg tcaggccgga tacgcagagg atcgtctcca aggtcaggtt
     6241 cttgccgagt cgttacgatc gaaactcgtc gagctggacg gagttgccga gaagcagcag
     6301 cgcctcgtcc tgcaggcgcc catcgatggc gtggtggttg atcaggccga ttcgctctat
     6361 cccggccgat ggatcaatga gaagctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnncga cagaagtggt cttcgtcgac cccacggttc cggattatga atctctgctc
     6541 gcgggcatgg ggcccaatgt ggaggtcgtt gtgctggatg cctcccgaga cggggtcgaa
     6601 cagatcgccg agtcgttaac cgggcgatcg ggcatcgatg ccattcatct gatctcgcac
     6661 ggcgacgctg gaacattgca agtgggcacc ggcacgttga ctgccgaatc gatgtcgact
     6721 cgttacgtgg acgagttagc aacagttcag caggctctgt cggagcaggc tgatattctc
     6781 gtgtacggct gtaattttgc cgagggggat gtcggccagg cggctgtggc ccggctggct
     6841 gaactgaccg gagccgacgt ggccgcgaac agcgatagaa ccggccacat cgatttgggt
     6901 ggcgattgga aattcgaaac ccaggttgga tcgatcgaaa ccgacctcgc gattactgat
     6961 gccgcgcaga tggactggaa ggggatcctg acgaccgaga ctgtcggcga ccaattctcc
     7021 ttaagttctt acgacaatca cgatgggact cagaagtggg cctcagattg gtccgaaacc
     7081 gatgccggcg gcggtggggc aagcggcggg gatgtgcatg tcgcttcatc acaactgagg
     7141 atcgacgccg gttccgtagg aaaccaagcg tcgcggcagg tcgatctgtc aggaacgagc
     7201 ggcgcaatcc tcacattcga ttacaccaac tcgttgtccg gctccgatca aatcgagatt
     7261 cgcgtgtcag ccgacggaaa gacgtatacc accctcaccg acggcgtctt ctcccctaag
     7321 tcgaatgccg gaagcggcac cgtcaagctc gacatttccg gttatgcctc cgcgagcacg
     7381 acgatccaat ttctcgtcac cggggtgggt gggggcgata gcctgtctgt cgataacgtg
     7441 caggtcagct atgacaccgg cgccgcgaac agtgcgccga cgatcagcag caacgggggt
     7501 ggaacgacgg cgtcggtcac ggtcggggag aacagcacgg tcgtcacgac cgtcacggct
     7561 actgacgttg atgcagggca caccctgggc tattcgatcg ccggtggagc agacgcaggt
     7621 cgattcacca tcaacaacag cacgggtcag ctgagttttg cctcggcacc cagctatgaa
     7681 gccccggcgg acagcggcgg gaataacgtg tacgacgtca cggtgcaggt gtctgacggc
     7741 cagggcggca cggataccca agccctcgcc gtgacagtgg caaacgtcaa tgaggcaccg
     7801 acagacctgg ccttgtcggc gaacacggtg gcggagaatg cggccaacgg gaccgtggtg
     7861 gggacggtga gcgggacaga ctcggacacc ggcgacacca ggacgtactc cctgaccgac
     7921 aacgccggcg gacggtttgc catcaatagc agcacgggcc agatcaccgt cgccaaccga
     7981 tcgctcctca attacgagag cgtcaccagc cacaacctga cggtgcgagt aacggacagc
     8041 ggcgggctca cctatgacga gaccttcacc atcaacgtga cggatgtgaa tgaagcggct
     8101 cccaggatca ccagcaatgg cgggggggcc acagccctca tcaatattgc ggagaatgcc
     8161 acggcggtca ccagggtcac tgcctccgac gccgatacca ggcaaaccct ggcctactcg
     8221 attgtcggtg gggccgatgc cgccaagttt acgatcaaca gtagcaccgg tgccttgtcg
     8281 tttgtgtccg ccccgaacta cgaggcgccg accgatagcg gcggtaacaa cgtctacgat
     8341 gtgacggtcc aagtgtcaga cggaagcggc ggcgtggact ctcaggccat cagcgtgacc
     8401 gtaactgctg tgaacgagag gcc
//
LOCUS       DAOQRM010000106         8290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_8159_length_8290_cov_18.419551, whole genome shotgun sequence.
ACCESSION   DAOQRM010000106 DAOQRM010000000
VERSION     DAOQRM010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8159_length_8290_cov_18.419551"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKK81_09080"
     CDS             complement(<1..491)
                     /locus_tag="PKK81_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV33071.1"
                     /translation="MWARRNSSQIQFILLFTLVVALPFTASRALAQEEAAAGAVVEKS
                     ARERDLRDQLQKILHELEELRQAPAGGATAAPVQPSVVQEPSEGKAAEAIPQFELADV
                     SIVSDRVQRRPEGITLSATEQSETESQPTRTLRESMESLPGVILRQANGPRDFSISIR
                     GSGV"
     gene            complement(601..921)
                     /locus_tag="PKK81_09085"
     CDS             complement(601..921)
                     /locus_tag="PKK81_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33072.1"
                     /translation="MSRLKTPSVGLAAALAGIYLVLAAFSMACAFDHADPMSAGHHHG
                     GTVSHSSFCNWACQANPTSDAAASALVLHPFLVVALFVERDHTVIAGGAGFHAASRAP
                     PVQS"
     gene            complement(918..1451)
                     /locus_tag="PKK81_09090"
     CDS             complement(918..1451)
                     /locus_tag="PKK81_09090"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV33073.1"
                     /translation="MMPTGLLRGLMLAVVLSTTAGPVWADDPFAALKIARVAPGTAAA
                     AFELQSLDGRSVQLADLKGKVVVVNFWATWCGPCKEEMPAFERLRRQLDPDRFVLLTI
                     TTDLQRAGIKQFLANLNVQLPVLFDEQQDVSQAYLVRALPTTVFIDRQGALLGRAVGP
                     REWDAPKAVQALQGLMP"
     gene            complement(1476..2753)
                     /locus_tag="PKK81_09095"
     CDS             complement(1476..2753)
                     /locus_tag="PKK81_09095"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNV33074.1"
                     /translation="MGQSETSLGGLLALCLAAAIPIAAAQAESAVPFELSQKFVTEHK
                     VKSLVGPSVQVDEQGQVSLAWMEEEKDVRSVLYARSAEAGGPMGAPVRINRPEDVPYW
                     RQEAPALVVQGEDVFVTWGLAHPKATPQQPFATELRLSRSIDGGKTFQPSVAVNDDPG
                     VIQHTFDALHRDADGRLHLSWIDGREGKKEPGTYVARSLDRGATITRNLKVDEGTCVC
                     CRTAVTSGPEGMVYVAWRKIFEGNVRETVVARSTDHGETFEAPVIVGHDRWVFPACPH
                     RPASMGVDRQGRLYVVWYTEGTDEIPAVYIAYSDDRGKSFSEKRKLNVSKNTFPDHPQ
                     IAVDPEGRIVVVWEEQAPVKRDVVMSVSMDRGATFTAPHKVNEKKSQTPVVAVNSQGL
                     FALAWMEHGMPGHKIVMQTVKVPSVKVAAEPVR"
     gene            complement(2776..3144)
                     /locus_tag="PKK81_09100"
     CDS             complement(2776..3144)
                     /locus_tag="PKK81_09100"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HNV33075.1"
                     /translation="MDRVSKDLRPVGFPILVCAGILAAWWIAAGCVWAGDGQAVGSDS
                     ARAQRIEELQKHRERIERELNQLKQQPDGVSRSSVPRTELSNQPTRTLKESMESVPGV
                     ASSPGAEGRSVDLSIRGAGK"
     gene            complement(3211..4344)
                     /locus_tag="PKK81_09105"
     CDS             complement(3211..4344)
                     /locus_tag="PKK81_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNV33076.1"
                     /translation="MENVLVAQSWQPKRSMTGWGASLLLHACLALAAFGLMPKMAMVV
                     EKEPFKWDVALVEAPREVVRQEEPAPVPQVQPPVPTPVKPRPEPVRAVPPPPQPVQRQ
                     VETRVVPQAIQREVQPVVETVRPQEQIQPTREVVQVQAKPIEPQEIQKTEPLMQAAVP
                     AEVVRETAPAVEQTVVEAASATAQTYQAQAVVSEPTVMETRPEPVVTASAPVVHAAPA
                     AEPAPASPAPAVEAVASVSEPVPAPPAPVAAVPAPVTAAQPDPATLQEHQVVARVATP
                     RPATKADYGWLAESLHRRIIELRHYPSTARLNGWEGKVVLKVSIRQDGQLKDVEVVKS
                     SGHESLDQAAMEAVRRACPLHMKHELTAPMVVLHLPVSYSLNR"
     gene            complement(4379..4837)
                     /locus_tag="PKK81_09110"
     CDS             complement(4379..4837)
                     /locus_tag="PKK81_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33077.1"
                     /translation="MKTQVDQRVGRMGLYGLVGLAIMLWAPGVSAADGKAEKSFTIAV
                     TDQQGVETELKNGIFYWEEKMSETSFVPHELRHLPAKRGTATVNIKFDQIKQIEIKPG
                     ADKTAPAMTVTLTNGKNGEFTPAVSGSFKGESDFGQVEVPVGSVSKVIFK"
     gene            complement(5083..>5478)
                     /locus_tag="PKK81_09115"
     CDS             complement(5083..>5478)
                     /locus_tag="PKK81_09115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNV33078.1"
                     /translation="IGWYTEGKDEQPRLFISTSDDQGATFSAPVALHNATTSLPDNLH
                     MAVHPDGRVVAVWEEVTGVRKRVVMRVSDDRGQTFSPMQTLSSGSKAEHPTVAIHDSG
                     TVALGWTEHAFPHNRIIVQQGQLSRVVTP"
     assembly_gap    5479..5578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5579..6099)
                     /locus_tag="PKK81_09120"
     CDS             complement(<5579..6099)
                     /locus_tag="PKK81_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33079.1"
                     /translation="MVPVREIGVAVCGAATLLALVGFSSGASAHNESQAVTMLGAPLT
                     LVQSTTALSPPTLRFHRDGRLHAAWIEKNGVQGEVKTAAISADHSAVAQVSVNPPGAG
                     PEAVHQSPGFAIGVDGEQFVTWSSANKTPGALFASDLRLARLDSGGARVQPPVQVNDD
                     GLAISHTFEHVIAG"
     gene            complement(6232..6537)
                     /locus_tag="PKK81_09125"
     CDS             complement(6232..6537)
                     /locus_tag="PKK81_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33080.1"
                     /translation="MRSSRFKSIVWLVAFAVIALLAVTTVNAVAHELQHAAHHDAGMH
                     GSGICAWMCATAGVHVVTPVHSTHLFTVAGDASFRSDTVLLPQRLAPSRPRAPPLFA"
     gene            complement(6634..>7101)
                     /locus_tag="PKK81_09130"
     CDS             complement(6634..>7101)
                     /locus_tag="PKK81_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV33081.1"
                     /translation="QANPGHADAWYLLGRLAERGNDLNAAAAAFKEAIAVKADLVDAH
                     YNLGFVYRSQGLLAEAEREFLEVIRYRPEYAEAHLNLGMVYTSLNRLEEAEKEHERAV
                     ALKPQSAEAHYNLGVFYELHRKDMGRALAQYRRYRDLGGRDERVERIIGMGGP"
     assembly_gap    7102..7201
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7413..>8290
                     /locus_tag="PKK81_09135"
     CDS             7413..>8290
                     /locus_tag="PKK81_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNV33082.1"
                     /translation="MLTQVLNIIFGSKNDREIKALRPIVERINGLESSFTPLSDQALA
                     EKTQEFKKRLEDGETLEDILPEAFAVCREMSRRRLNMRHFDVQLIGGMILNKGRIAEM
                     KTGEGKTLVATLPLYLNALEGKGAHLVTVNDYLAKRDAQWMAQLYHALGLSVGIIQHD
                     ASFLYDPTYEASDKRLQHLRPCTRHEAYRAEITYGTNNEYGFDYLRDNLIVSDLSQCV
                     QRPLNFAIVDEVDSILIDEARTPLIISGPTDQTTDLYYRINSIIPQLKAEHDYTIEEK
                     TKTASLTEEGNVRVEKL"
BASE COUNT         1625 a         2715 c         2260 g         1490 t
ORIGIN      
        1 acgcccgagc cacggatcga aatactgaaa tccctcggtc cgttggcctg tcgcaggatc
       61 acgccgggga gcgactccat cgactcacga agcgtcctgg tcggctgcga ttcggtttcc
      121 gactgctccg tcgccgacag cgtgatccct tccggacgcc gctgcacccg gtcgctcacg
      181 atgctgacat cggccagttc aaattgcgga atcgcttcgg cggcttttcc ttccgacggc
      241 tcctgcacca ccgacggctg taccggcgcg gcggtcgcgc cacctgcagg cgcctgtcgg
      301 agttcctcca gttcatgaag aattttctgt agctgatccc tcaagtcccg ctcccgcgcg
      361 cttttttcaa cgaccgcgcc ggccgccgct tcctcctgcg caagtgcgcg ggaggctgtg
      421 aacggcagtg ccactaccag ggtgaacagc agaatgaatt gaatttgaga tgaattccga
      481 cgggcccaca tacacgtcct cccggcacaa ctgtgcacac gacagcagag acataccctt
      541 cccgcccctg tctggccgga cgagaaaagc tgctagaaca cctagcgatc tgaaatggaa
      601 ttacgattga acgggcggcg cgcgagaggc ggcgtggaag cccgcgccgc cggcgatgac
      661 ggtatgatcg cgctcgacaa acagcgcgac cacaaggaac ggatgaagca ccagtgcgga
      721 cgccgcagca tccgacgtgg gattggcctg acaagcccaa ttacagaaac tggagtgaga
      781 gaccgttccg ccatgatgat ggccggccga catggggtcg gcatggtcga aggcgcaggc
      841 catggagaac gccgccagga ccagatagat ccccgcaagt gcggccgcga gtccgacgga
      901 gggtgtcttg agacggctca tggcatcagc ccctgcaacg cctgcaccgc cttgggcgca
      961 tcccactcac gcggtccgac agcgcggccc agcaaggccc cctgccggtc gatgaacacc
     1021 gtggtcggca aggcccgcac cagataggct tgcgagacat cctgctgctc gtcgaacaac
     1081 acggggagtt gcacgttgag attagccagg aactgcttga tgccggcgcg ctgcaagtcg
     1141 gtcgtgatgg tcagcaacac gaagcggtcg gggtccagct gccgtcgcag acgctcaaag
     1201 gccggcattt cttctttgca gggcccgcac caggtggccc agaaattcac cacgacgact
     1261 ttccccttca agtccgcgag ttgcaccgac cggccatcca gcgactgtag ctcaaaggcc
     1321 gcagcggcag tgcccggtgc aacccgcgcg attttcaacg cggcgaaagg atcatccgcc
     1381 catacaggac cggccgtcgt gctcagcaca accgccagca tgaggcctct caacaatccc
     1441 gtcggcatca tcgttccaat cacaaaccca cccagctaac gcaccggttc agccgccacc
     1501 ttcaccgaag gcaccttcac ggtctgcatc acaattttat gacctggcat gccatgctcc
     1561 atccaggcca aggcaaacag gccctgacta ttcaccgcca cgaccggagt ctggcttttt
     1621 ttctcgttta ccttgtgcgg cgccgtaaac gttgccccac gatccatcga gacgctcatc
     1681 accacatccc gcttgaccgg agcctgctcc tcccacacca ccacgatccg tccttcggga
     1741 tcgaccgcaa tctgcggatg gtcgggaaag gtgtttttcg agacgttcaa cttccgcttt
     1801 tcagaaaaac tcttcccccg atcgtccgaa taggcgatgt agacggcggg gatttcgtcc
     1861 gttccttcgg tgtaccagac gacgtagaga cgaccctgac ggtccacacc catcgaggcc
     1921 ggacgatgcg ggcaggccgg gaacacccat cgatcatgcc ccacgatcac gggcgcctcg
     1981 aaggtctccc cgtggtccgt cgaacgggcg accacagttt cacgaacatt gccttcaaaa
     2041 atcttgcgcc aagctacgta gaccatgccc tcaggaccac tcgtcaccgc cgtgcggcag
     2101 cagacacagg taccctcgtc caccttgaga ttcctggtga tcgtcgcgcc acggtccaac
     2161 gaacgggcca cgtaggtacc cggctccttc tttccttcac gcccgtcgat ccaggagagg
     2221 tgcaaccgtc catccgcatc acgatgcaac gcatcgaagg tatgttgaat gacccccgga
     2281 tcgtcgttca ccgcgacgga gggctgaaac gtcttgcccc catcgatcga gcggctcaat
     2341 cgcaattccg tcgcgaaggg ctgttgcggc gtggctttcg gatgggccag gccccaggtg
     2401 acaaacacgt cttccccctg cacaaccagc gccggcgctt cctggcgcca atagggcacg
     2461 tcctccgggc ggttgatgcg caccggcgct cccatcggcc cgcctgcctc cgcgctgcgg
     2521 gcgtacagaa ccgaccgcac atccttctcc tcttccatcc aggccaagga gacttgcccc
     2581 tgctcatcca cctgcacgga cggaccgacc aggctcttca ccttatgttc ggtcacaaac
     2641 ttctggctga gctcgaaggg aacggccgac tccgcctgcg cggccgcaat agggatcgcc
     2701 gcagcgaggc acagggccaa cagaccgccc aggcttgttt cagactgtcc catcactgcc
     2761 ctttccttcc cacattcatt ttccggctcc tctgatggag agatcgacgg atcgtccctc
     2821 tgctcccgga gacgacgcaa cccccggaac cgactccatg gattctttca gcgtccgcgt
     2881 gggctggttg ctcagttcgg tcctcggcac cgacgagcgg ctgaccccgt ccggctgctg
     2941 tttcaattga ttcaattccc gctcaatgcg ctcccgatgc ttctgaagtt cctcgatccg
     3001 ctgagcccga gcagaatcgg aaccgacggc ctgcccatct ccagcccaca cacagcccgc
     3061 ggcaatccac caggccgcga gaatccccgc acagacaaga atcgggaaac cgaccggtcg
     3121 cagatcttta cttactctat ccatgatcag cgacttactc tttcccggac ggtccgcagg
     3181 atggtcaaca tggtcatcca gcgaggcggc ctagcgattc aaactgtaac tgaccggcag
     3241 atgcagcacg accatcggcg ccgtcaactc atgcttcata tgcagaggac aggcgcgccg
     3301 caccgcttcc atcgccgcct gatccaacga ctcatgccct gaacttttca ccacctcgac
     3361 gtctttgagt tggccgtcct ggcggatcga gaccttcaag acgactttgc cttcccagcc
     3421 gttcaagcgc gcggtgctgg gataatgccg gagttcgatg atgcggcgat gcagcgattc
     3481 ggccaaccag ccataatcgg ctttggtggc gggcctcggc gtcgccacgc gcgccacgac
     3541 ttgatgttct tgtaacgtcg ccggatccgg ctgcgctgcc gtcactggcg cggggacggc
     3601 agcgaccggg gccggaggag caggaaccgg ctcactgacg gacgcgacgg cctccacggc
     3661 cggagcggga ctcgccgggg ccggttcagc cgccggagca gcgtgcacga ccggcgcact
     3721 cgccgtaaca accggttcgg gcctcgtctc catcaccgtc ggctcgctga ccaccgcctg
     3781 ggcctgatag gtctgagccg ttgcagacgc cgcctccaca accgtctgct cgactgccgg
     3841 agcggtttcc cgtaccacct cagccggcac cgccgcttgc atcaacggct ccgttttttg
     3901 aatctcttgc ggctcgatgg gcttggcttg cacctgcacc acttccctgg tcggctgaat
     3961 ctgttcttga ggacgaaccg tttcgacgac cggctgcacc tcacgttgga tcgcctgcgg
     4021 cacgacccgc gtttccacct gacgttgcac cggctgaggc ggcggtggca cggcacgtac
     4081 cggctcaggt cgcggcttca ccggagtcgg aacgggaggc tgcacctgcg gcaccggagc
     4141 cggctcttcc tggcgcacga cttcgcgcgg agcctcgacg agggccacgt cccatttaaa
     4201 cggctctttc tccaccacca tggccatctt cggcatcaga ccgaatgcag cgagcgcgag
     4261 acaggcgtgc agcaggagcg acgctcccca ccctgtcatc gaccgcttgg gttgccacga
     4321 ttgcgccacg agcacattct ccatgggaca ctccctttcg aaacgatacg ttccgccctt
     4381 acttgaagat caccttggac accgacccga ccggcacctc gacctggccg aaatccgact
     4441 cgcccttgaa gctgccgctg acggcaggcg taaattcccc gttcttgccg ttcgttaagg
     4501 tcaccgtcat ggccggcgca gtcttgtcgg caccgggctt gatctcaatc tgcttgatct
     4561 ggtcgaactt gatattgacc gtcgccgtgc cgcgcttggc cggcaggtgc cgcaactcat
     4621 gcgggacgaa cgaggtctca ctcatttttt cttcccaata gaagatgccg ttcttcaact
     4681 cggtctcgac cccctgctgg tcggtcacgg caatggtgaa cgacttctcc gcctttccat
     4741 cggcggcgga cacgcccggc gcccacagca tgatcgccaa tcccaccaac ccgtacagcc
     4801 ccatccgtcc cacccgctga tcgacctgcg tcttcatgac tcctcccctt gctcctgcag
     4861 aatgctgaaa aagtccgcca gcttcgttct cgcaacgttc agagactcac cgtgcgggaa
     4921 cagtacgctt cgccccttcg cttgctgcgg ccttgctgaa cggcctttgt gagcatcctg
     4981 cgaattatcc cgtcgttgtc gtcgcacttc agataacgac ggctattgaa cgaaccccga
     5041 gtgtttccgc aacctgctac aaataggaat cgccggaccg cgctagggtg tcaccacgcg
     5101 cgacagttgc ccctgttgta cgataattct gttgtgcgga aaggcatgtt cggtccagcc
     5161 caacgccacc gttccactgt cgtggatggc cacggtcgga tgctcggcct tggagccgct
     5221 gctcaaggtc tgcatcgggc tgaatgtctg cccgcgatcg tcagaaaccc gcatcaccac
     5281 acgcttccgc acccccgtca cttcctccca caccgccacg acccggccat ccggatggac
     5341 ggccatgtgg agattgtccg gcaaagaggt tgtggcattg tgtagcgcta ccggcgcgga
     5401 aaacgtcgcg ccctgatcgt ccgatgttga aataaagagg cgcggctgtt cgtccttgcc
     5461 ctcggtgtac cacccgatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     5581 ggcaatcaca tgctcaaagg tatgcgaaat ggccaagcca tcgtcattga cctgcaccgg
     5641 cggctgaaca cgcgcgcccc ccgagtccaa tcgcgcgagg cggagatccg acgcgaataa
     5701 ggctccgggt gtcttattgg cagaggacca ggtgacaaac tgttctccgt ccacgcctat
     5761 ggcgaaaccc ggtgattgat gcacggcttc cggcccggca cccggcggat tgacggaaac
     5821 ctgggcgacc gccgagtgat cagcggaaat agctgcggtc ttcacctccc cctgcacccc
     5881 gtttttttca atccaggccg cgtgcagcct tccatcgcga tgaaaccgaa gtgtgggggg
     5941 agagagggcc gtggtggact ggacgagtgt gagcggcgcc ccaagcatcg tgacagcctg
     6001 ggattcattg tgggcagagg ctccgctgct gaagccgacg agggccagca gggtcgcagc
     6061 accgcacacg gcaacgccga tctcacgcac agggaccatc acagtgtcct cgtattccga
     6121 cgatctgaaa gaaggaatgc acccacagca tgcgagacac ctcgccgaac tgtttcctcg
     6181 ggctctgcga catcgagtca cggagcgaga caggcacaac ggagacttgg attaggcgaa
     6241 caggggtgga gcgcgaggcc gactcggggc gagtcgctgg ggcaagagca cagtatcaga
     6301 gcggaacgaa gcgtcaccgg ccacggtgaa cagatgcgtc gagtgaacgg gggtcaccac
     6361 gtgcaccccg gccgtcgcac acatccaggc acaaatgcct gacccatgca tcccggcatc
     6421 gtgatgcgcg gcatgttgaa gttcgtgcgc gaccgcgttc acggtcgtca ccgccaataa
     6481 ggcaatgacg gcgaatgcga ccaaccagac gatgctttta aaacgggaac ttcgcataag
     6541 gacaggccca aaatctggcc cgcatgctaa caaactgacc gacggagcgt caacgccact
     6601 tttggcctcc aagggccatt cggccggggc cggttacggt ccgcccatgc cgatgatgcg
     6661 ctcgacccgc tcgtcgcggc cgccgaggtc ccgatagcgc cggtattgcg ccagcgcccg
     6721 ccccatgtcc ttgcgatgaa gctcgtaaaa gaccccgagg ttatagtggg cctccgccga
     6781 ttgaggcttg agagccaccg cccgctcgtg ctccttctcc gcctcctcca gccggttcaa
     6841 gctggtgtag accatgccca gattcaagtg cgcttccgca tattcggggc gataccggat
     6901 cacctccaga aattcccgct ccgcctcggc aagcagcccc tgactccgat agacaaaccc
     6961 caggttgtaa tgggcatcga ccagatcggc cttgaccgca atggcttctt tgaatgcagc
     7021 ggcggcggcg ttgaggtcgt tgccgcgctc ggccagtcgc ccgaggagat accaggcatc
     7081 cgcatgcccg gggttcgcct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nccggcgaag cggtgagccc cactataaat cgcagcgcat gcgaaagcaa cgacaggagc
     7261 ccgtcggccc cgttcattgc aaagcagttg agacctccgg cgctgtttgg gtaggattcc
     7321 cctatggatt cctgtatact ccgccgccga gcccatgctc aaagggtgag tctcgcccgt
     7381 gagaaggctc atgtgaataa aggtacgtct gcatgttgac ccaagtcctg aacatcatct
     7441 tcggaagcaa aaacgaccgc gagatcaaag cgttgcgccc gatcgtcgag cgtatcaacg
     7501 gactcgaatc cagcttcacg cccttgtccg accaggcact ggccgagaag acgcaagagt
     7561 tcaagaaacg gctcgaagac ggagaaacgc tggaagatat tctgcccgaa gcgttcgccg
     7621 tgtgccggga aatgtcccgt cgtcgcttga atatgcggca tttcgacgtg cagctcatcg
     7681 gcggcatgat cctgaataaa ggccggatcg ccgaaatgaa gaccggtgag ggcaagaccc
     7741 tggtcgccac cttgcccctc tatttgaatg cgctcgaagg caaaggcgcc cacctggtca
     7801 ccgtcaacga ttacctggcc aaacgcgacg cccaatggat ggcgcagctc tatcacgcgc
     7861 tcggcctctc cgtcggcatc atccagcacg atgcgtcctt tttatatgat ccgacctatg
     7921 aagcgtcgga caagcgcctg caacatttgc ggccctgcac cagacatgag gcctaccgcg
     7981 ccgagatcac ctacggcacc aacaacgaat acgggttcga ttacctgcgc gacaacctga
     8041 tcgtcagcga cctgagccaa tgcgtccaac gcccgctgaa cttcgccatc gtggacgagg
     8101 tcgacagcat tctgatcgac gaggcgcgta cgccattgat catctccggt cccaccgatc
     8161 aaacgaccga tctgtactac cgcatcaatt cgatcatccc gcagctcaag gcggagcacg
     8221 actatacgat cgaagaaaag accaagaccg cctcgctcac ggaagagggc aacgtccgtg
     8281 tggagaaact
//
LOCUS       DAOQRM010000107         8211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_8272_length_8211_cov_12.901177, whole genome shotgun sequence.
ACCESSION   DAOQRM010000107 DAOQRM010000000
VERSION     DAOQRM010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8272_length_8211_cov_12.901177"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKK81_09140"
     CDS             complement(<1..290)
                     /locus_tag="PKK81_09140"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV33083.1"
                     /translation="MTMKVHYQVSLSKESTTSGEGRLLDLSVEGCRIEGAHHLPVNTY
                     LSLRLLISPKEMPVLVDLAAVRWTRGIVCGIHFLSLQPLQTARLKTFLASAAP"
     gene            780..1151
                     /locus_tag="PKK81_09145"
     CDS             780..1151
                     /locus_tag="PKK81_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33084.1"
                     /translation="MKASFHIIIIPFTLTGMLMAGVAFGNPVMLPQHPGYPMGKATDP
                     VNGQSLANDPGRANAVGEKALNGAAAFDDRHVSQQLPKDEQNQRLLEKPGAGILPKVQ
                     GPQIVIDPPVKEGTKVQASPQ"
     gene            1304..2026
                     /locus_tag="PKK81_09150"
     CDS             1304..2026
                     /locus_tag="PKK81_09150"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNV33085.1"
                     /translation="MSTKSRVQKSPRRKKKSPQSSVPLARGLKQRFQNRLLKWYKEHG
                     RDLPWRRTSDPYHILVSEVMLQQTQVDRVIPKYHEFLERYPSFEQLADAPVAEVKQTW
                     YPLGYNIRPERLHSIACETVARYGGQLPNDAEELLSFKGIGRYTAGAIRSFAFNEDAP
                     ILDTNVIRVLHRVFIAQGDPKAQKAALWELSETLIPRGKGYDFNQALMDFGATVCTAR
                     DPYCLLCPMKPFCKTYPFDPAR"
     gene            2144..2539
                     /gene="mutT"
                     /locus_tag="PKK81_09155"
     CDS             2144..2539
                     /gene="mutT"
                     /locus_tag="PKK81_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HNV33086.1"
                     /translation="MMEVIEVAAGIIVHEGRYLIARRKAGVHLGGLWEFPGGKRETGE
                     TLEECLHRELREELNVRIGPPTPFQVVRHEYPEKIVELHFFRCRIETGVAIALDCAEL
                     RWVYPHEMAAFEFPSADQPIIAALQQGKV"
     gene            2546..3262
                     /locus_tag="PKK81_09160"
     CDS             2546..3262
                     /locus_tag="PKK81_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNV33087.1"
                     /translation="MKVVLDIETVQAPREEWARLAGRQLASGEAAFSETGGDLFAVGE
                     AQAQHRLDEELYEKSSFDGTFSRIVCIGLLEFSDNLEPRGATSWYGANEQELLRRFWS
                     HLGQLRPSLFITHNGLNFDLPFIKKRSIIQQVKPTMEINLAKFRAEPVYDTMAIWSNW
                     DNRGWVKLDVLARALNVESKSGSGSQVAQMWADGQGKEIALYCLQDTYVTYGCYCRMN
                     FRQPISREIVLLRPEVIDVG"
     gene            complement(3279..4325)
                     /locus_tag="PKK81_09165"
     CDS             complement(3279..4325)
                     /locus_tag="PKK81_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33088.1"
                     /translation="MRIHGSRTPFTKTLNSGLLGLTLLGAMAAGCSSTAATVQQKAAA
                     QPQGSVRLEEVADWEFEASHPSTIALATLDSLLRGVMISDAQTDLSNLPVDGSKPMTV
                     FSDEDVHYLAPLLAQALSQAQPEYVVAFRLSSSAGSGSEPTAGTLYVQNERLYLTVTE
                     HNGTLAKVESSLFSSGRPARIVSITPESAGKIEQALPAIAQGRQPLKSLAIDYKLFAK
                     NPEPAPAVAATIAAPAPQPVQTSAPRMVVASVVEEAPVKNAPPAQPAGPASHASKASS
                     AEDERLTETLQELQDAREAMARKDAKISALRRDLESMRTQLETKDKELRAVKSTPQTQ
                     PKRETRNKAELTVR"
     gene            complement(4411..4542)
                     /locus_tag="PKK81_09170"
     CDS             complement(4411..4542)
                     /locus_tag="PKK81_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33089.1"
                     /translation="MKKDIAQSHAEEAPRGMSLETIIAVGVFGWMALGVVMMGLGIW"
     gene            complement(4995..6353)
                     /gene="miaB"
                     /locus_tag="PKK81_09175"
     CDS             complement(4995..6353)
                     /gene="miaB"
                     /locus_tag="PKK81_09175"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNV33090.1"
                     /translation="MTQPNKPHTVHIETFGCQMNEYDSELVRSLLRKAGFEFTEDRER
                     ADVLLMNTCAIRENAHNKVYGHLAELKAVKEQRPLVVGVLGCMAQNLKEELTEKQPLV
                     DVLVGPDGYRQLPGLLANALNAEEQGLVRRGMAVDLSEYETYDDILPERDGSSNAWIA
                     IMRGCDNFCSFCVVPYTRGRERSRDPQGILREVEASVATGHTQITLLGQNVNSYRYED
                     WDFARLILAVAEVPGVRRVRFTSPHPKDFPAALLDAVAGHPNICKHIHLPLQSGNDRI
                     LELMNRTYSRKEYLDLAAHIRRRHPGIALTTDIICGFCSETEEEFLDTYRVVEEVQYH
                     SAYVFKYSERKNTIAARKFPDDVPEAVKGERVSRLVDLQRPITARLNRELIGQTLPVM
                     VEGDSKRSSDQWMGRTDTGVYVIWNKSDAPALLGSIQPVTILDGSAAVLMGRREPSAI
                     VA"
     gene            complement(6350..7681)
                     /gene="mtaB"
                     /locus_tag="PKK81_09180"
     CDS             complement(6350..7681)
                     /gene="mtaB"
                     /locus_tag="PKK81_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNV33091.1"
                     /translation="MPRASLHTLGCRLSQSETSMLADTLARRGYRLVEFGKETDLLVL
                     NTCSVTENAEKDCRYAVRKTLRHSPHAFVAVTGCYAQTGATQLQKVPGIDLIVGTQFK
                     MNLPDYLPAPAKLLKQPEPELRHTRTIDREDFVLPGTAYSDSTRALLKIQDGCDFMCS
                     FCLIPFARGRERSRTAEDVLREARELAAHGYRELVLTGVNIGQYSYQGLGLVELLREL
                     DAIPDVTRIRISSIEPTTVPAALLEHMAGSTKLCHYLHLPLQSGDDGILQAMNRRYAV
                     REYEELVEQAIALMPDLGLGTDLMVGFPGEDELAFANTVRTVERLPFSYFHVFSYSAR
                     PGTAAARLEDQIPPAVIRQRSKALAELSRTKALAFYQQQIGRTLPVLFEQGERDGFRT
                     GTTANFTRVAVAADAVEAGSIHQVTITGIMDGLAYGRPVATVLAPSHRPLL"
     gene            7838..>8211
                     /locus_tag="PKK81_09185"
     CDS             7838..>8211
                     /locus_tag="PKK81_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33092.1"
                     /translation="MTNLRVAASLLLAVLLFIPATSAWSQDPLPIEPDLNSRLDELYD
                     HEARLFIMLYSLHGDGKVDYITGRLVQEYTRSNYGNPVYYTEPYPLFYWWDHTMFNDP
                     DQDGVNGNERVYQENIEFDIAR"
BASE COUNT         1584 a         2282 c         2614 g         1731 t
ORIGIN      
        1 ggcgcggcgg aggcgagaaa cgttttcagc cgtgcggtct gtaacggctg gagcgagaga
       61 aagtgtatgc cgcagacgat tccacgcgtc catcgcacgg cggccagatc cacgaggacc
      121 ggcatctcct tcggtgaaat gagcaggcgt agcgaaaggt aggtgttgac gggcaaatgg
      181 tgcgcaccct cgatgcggca gccttccacc gagagatcca gaagccgtcc ttctccgctt
      241 gtggtcgact ccttcgagag agagacctga tagtgaactt tcatggtcat gtgccggtct
      301 gtatccttac gtgaattgaa tcatcgcgcg agctgtcgaa tctgtagtcc ggcgggaggc
      361 ggtgcgtaga gccgcgccat cagcgtcgga aaccgcctcg tgatctctgt aatggggagc
      421 gagcaacgag cgcggataat gtgtttcgtc agaaggacat aacatgtccc taatcgcggc
      481 gcgatcagga taatgtcccc gaccatttgg agttgggaaa aaccctaggg tatgggaggg
      541 cctgcgccgg gacttttccc ggcgtcgtgg atgttgttac gaggttgggc gggcttgatt
      601 gttgggttca acgagagcaa gctgaataca atagtcttgg aggtgaccat gaagccgggg
      661 tgacctcgat ccaaccacgc tcgtcagttc cctgttattc catcgatgtc ttcctgcgcc
      721 cttctgatcg tcctgacagg agggcgtttt catttcaccc gcttaacagg aggtttgata
      781 tgaaagcctc attccatatc attatcattc catttactct gaccggaatg ctgatggccg
      841 gcgtggcgtt cggcaaccct gtgatgttgc cgcaacatcc cggctatcca atgggcaagg
      901 cgactgatcc agtcaatggg cagtctcttg ccaacgatcc gggccgcgcc aatgcggtcg
      961 gggagaaggc gctcaacgga gcggcggcgt tcgacgaccg gcatgtgtcg caacagcttc
     1021 cgaaagacga gcagaatcag cgactcctgg aaaagccggg ggccgggatc ttgccgaaag
     1081 tgcaaggccc tcagattgtg atcgatccgc cggtgaagga agggaccaag gtgcaggctt
     1141 cgccgcagta gcgagcgata tcacgagtac tgccggagac cgcagggcgc gaggcaagga
     1201 tttgttttcg tggatcgcgc tctgcggttc cgccgtcgag gcgtcgtagt ttgattttca
     1261 gcccgcccgc ctcttagaat gccgccactc atcacgaact gttatgtcta caaagtctcg
     1321 cgttcagaaa tccccgcgcc gtaagaagaa gtcgcctcag tcctcggtcc cgcttgcacg
     1381 cgggctgaag cagcgttttc agaaccggtt gttgaagtgg tacaaggaac atgggcgtga
     1441 cctaccctgg cgcaggacct ccgatcctta tcacatcctg gtgtcggaag tgatgctcca
     1501 gcagacccag gtcgatcgtg tgattcccaa gtaccatgaa tttttggaac ggtatccgtc
     1561 gtttgaacag ttagccgatg cgccggttgc ggaggtcaag cagacctggt atccgctggg
     1621 gtacaacatc cggccggaac ggttgcacag catcgcttgt gaaaccgtag cccgctacgg
     1681 cgggcagttg ccgaacgatg cggaggagtt gttgtccttc aagggcatcg ggcggtacac
     1741 cgcgggggcg attcgttcgt ttgcgttcaa cgaagacgct ccgattctgg acacgaacgt
     1801 gatccgcgtg ctgcatcgtg tgttcattgc gcagggcgac cccaaggcgc aaaaggccgc
     1861 cctctgggag ttgtctgaaa ccctgatccc gcgcggcaag gggtatgact tcaatcaggc
     1921 cctcatggat ttcggcgcta cggtctgtac ggcgcgcgac ccctactgcc tcctgtgccc
     1981 gatgaaaccg ttctgcaaga cctatccgtt cgatccggcc aggtagcgga tcaggagcat
     2041 atggcgcata gccaatggcc catagtttgg aaccgagacg tacctgcttt tcagtgccga
     2101 gctacatgcc ataggccata cgttcttctc ccggacgcgg tgcatgatgg aagtgatcga
     2161 ggttgcggca gggatcattg ttcacgaggg tcggtatctg atcgcgcggc ggaaggccgg
     2221 ggtgcatctc ggcggcttat gggagttccc gggcgggaaa cgagaaacgg gcgaaacgtt
     2281 ggaggagtgt ctgcatcggg agttaaggga ggagctgaat gtgcgtatcg gccctcccac
     2341 tccctttcag gtcgtacgac atgaatatcc cgagaagatt gtggaattgc atttctttcg
     2401 ctgccggatc gagaccggtg ttgcgatcgc gctggactgc gcggaacttc gatgggtcta
     2461 tcctcatgag atggccgcgt tcgagtttcc ttcggccgat cagcccatta tcgcggcgct
     2521 tcagcagggg aaagtgtagg ggggtatgaa agtggtcctc gacatcgaaa cggtccaggc
     2581 tccccgcgag gaatgggccc ggctggccgg tcgtcaactg gcctcggggg aggcggcttt
     2641 ttccgagacc ggaggggatc tattcgcagt cggggaggcg caggcgcagc atcggctcga
     2701 tgaagaactc tatgaaaaat cctccttcga cggaaccttc agccgcatcg tgtgcatcgg
     2761 gttgctggag ttttccgaca acctcgaacc gcgtggcgcc acctcctggt acggggcgaa
     2821 cgagcaggag ctattacgtc gtttctggag tcatctcggg cagctccggc cctcgttgtt
     2881 cattacccac aacggcctga atttcgacct gcccttcatc aagaaacgct cgatcattca
     2941 gcaggtgaag ccgaccatgg agatcaacct ggccaagttt cgggcggagc cggtgtacga
     3001 caccatggcg atctggagca actgggacaa tcgtgggtgg gtcaagctcg acgtgctcgc
     3061 gcgggcgttg aacgtggaaa gtaaatcggg cagcgggtcg caggtggccc agatgtgggc
     3121 ggacgggcag ggcaaggaaa tcgctctcta ctgcctgcag gatacctatg tcacgtatgg
     3181 ctgctattgc cgcatgaatt tccggcagcc gatctcacgg gagatcgtgc ttctgcggcc
     3241 ggaagtcatc gatgtgggct gaatgaacgg gcaggcggtc agcgaaccgt gagttcggcc
     3301 ttgttccggg tctcgcgttt gggttgagtc tgcggggtgc tcttcacggc ccgaagttcc
     3361 ttgtccttcg tttccaactg cgtacgcatc gattccagat ctcttcgcag ggcgctgatc
     3421 ttggcgtctt tccgggccat cgcttcccgc gcgtcctgta actcctgaag tgtttcggtc
     3481 aatcgttcat cctcggcgga agaagctttc gacgcatggg atgccgggcc tgccggctgc
     3541 gcaggggggg cgttcttaac cggcgcctct tctaccaccg aggcgacgac catccggggt
     3601 gcagaggttt ggaccggttg aggcgccggc gctgcgatcg ttgccgcgac cgccggggcc
     3661 ggttctgggt tcttggcaaa cagtttgtag tcgatggcga gggatttgag cggctgccgg
     3721 ccctgcgcaa tcgccggtaa tgcctgctcg atttttccgg ctgattccgg cgtgatgctc
     3781 acgatgcgag ccggtcgccc gctgctgaac agggaggact cgactttcgc cagcgtaccg
     3841 ttgtgctcgg tcacggtgag atacaaccgt tcgttctgaa cgtaaagggt gcccgcggtg
     3901 ggttcagagc ctgaccctgc cgatgaggat agacggaaag cgaccacata ttcgggttgc
     3961 gcttgagaca acgcttgggc tagcagggga gcaagatagt gcacgtcctc atcgctgaac
     4021 actgtcatcg gcttgctgcc atccacgggc agattcgaga gatccgtctg tgcgtccgag
     4081 atcatgacac cacgcagcag gctatcgagg gtggcgagag cgatggtgct ggggtggctg
     4141 gcctcaaatt cccaatcggc gacttcttcg agccggacag aaccctgggg ttgcgcagcg
     4201 gccttctgtt gaacggtggc ggcggtcgat gagcaaccgg cagccatggc tcccaacagg
     4261 gtcaacccga ggagtccgga gttcagggtc ttggtgaaag gagtgcggga gccgtgaatt
     4321 cgcatggcgg tcatccttct cggctggtcg atgcggtgcg aacagaggcg gcgcggcttc
     4381 aggacgaagg cgcgtcgtaa cgaatcggga ctaccagatt cccaagccca tcatcaccac
     4441 gccgagggcc atccacccga agaccccgac ggcgataatg gtttccaggc tcatgccccg
     4501 aggggcctct tctgcatgac tctgggcgat gtctttcttc atgatgcgaa atcctctggg
     4561 gccggtcggt ggttgatgaa cctggttaat tgtggacgcc agaatggacc aagcaagggc
     4621 tatgccctct ttccatggac ttcggagacc ctagtggtaa tcccctgaga gtcgatctct
     4681 gctgtggctg cggctgatgc gacgtgactg atcgcggcga aagatgccgt ctaagctatt
     4741 gaagtttcaa ggcggtgaag tcgctccatt gagaggcagg tgatggtgag ccgacacgcg
     4801 agccaggcgc tgaagacggc gataggcgcc gatgcctgct cttttcccgt cagtctgacg
     4861 agtgcgggtg tctaaaaatt ggcaactgtt gttcaggact gggcaagaat gtcgccgttc
     4921 gacggcaatc tttggtctcc cgtagacctg tcgcaggcgg ccgggcgatg agttgtgcgg
     4981 agtgcgggga taattcacgc gactatcgca gagggctcgc gccgtcccat caggactgcg
     5041 gcgctgccgt cgagaatagt gaccggttga atgctgccga gtaacgccgg ggcgtcgctc
     5101 ttgttccaaa tgacgtacac gccggtatcg gtgcggccca tccattgatc gctggagcgt
     5161 ttggagtcgc cttcgaccat caccggcagg gtctgtccga tcaattcccg attgaggcgc
     5221 gcggtgatag ggcgctgcag gtccaccagg cggctgaccc gttcgccctt cacggcctcc
     5281 ggcacgtcgt ccgggaactt ccgcgccgcg atggtatttt tccgctccga atacttgaac
     5341 acataggcgg agtggtactg cacctcctcg acgacccgat aggtatccag aaactcctcc
     5401 tcggtttccg agcaaaagcc acagatgata tcggtggtga gcgcgatccc gggatggcgg
     5461 cgtcgaatat gcgcggccag atcgagatat tctttccggc tgtaggtccg gttcatgagt
     5521 tccaggatgc ggtcgttgcc ggattgcagg ggcaggtgga tatgtttgca gatgttcgga
     5581 tggccggcga cggcatccag caacgcagca ggaaagtcct tcgggtgcgg agacgtgaag
     5641 cggacccgcc ggactcccgg cacctcggcg accgcaagga tgaggcgggc aaagtcccag
     5701 tcttcatacc ggtagctgtt cacgttctgt ccgagcaacg tgatctgcgt atgaccggtg
     5761 gcgaccgacg cctcgacctc gcggaggatt ccctggggat cgcgtgaacg ctcgcgccct
     5821 cgcgtgtagg gcaccacgca gaagctgcaa aagttgtcgc aaccgcgcat gatggcgatc
     5881 caggcgttgc tgctgccgtc gcgctcgggg aggatgtcgt cgtaggtttc atattcggac
     5941 agatccacgg ccatgccccg tcgcaccagg ccctgttctt ccgcattcag cgcattcgcc
     6001 aatagtcccg gcaactgccg gtatccgtcc gggcccacga gcacatccac cagtggttgt
     6061 ttctccgtca gctcctcctt gaggttttgc gccatgcagc cgagcacccc gacgaccagc
     6121 gggcgctgtt ccttcaccgc cttcaactcg gcgagatggc catagacctt gttatgcgca
     6181 ttctcacgga tggcacaggt gttcatgagc aggacatcgg cgcgctcgcg atcttcggta
     6241 aactcgaatc ccgctttgcg gagtaacgac cgcacgagtt cggaatcgta ctcgttcatc
     6301 tggcagccga aggtttcgat atggacggta tggggcttat tgggttgtgt cataaaagtg
     6361 gcctgtgcga gggcgctaag actgtggcga ccggccggcc ataggccagc ccgtccatga
     6421 tgccggtgat ggtgacctgg tggatcgagc cggcctcgac ggcatcggcc gcgaccgcca
     6481 cccgggtgaa gttggctgtg gtgccggtgc gaaatccatc gcgttcccct tgttcgaaga
     6541 ggacggggag ggtgcggccg atctgttgct gataaaaagc cagcgctttc gtccgtgaca
     6601 gttccgccag ggccttgctg cgttgccgga tgacggcagg cggaatctgg tcctccagtc
     6661 gcgcagctgc cgtacccggc cgcgccgaat aactgaagac atggaaatag gaaaagggga
     6721 gtcgctcgac tgtccggacc gtattcgcga acgccagttc gtcttcgccg ggaaatccga
     6781 ccatgagatc ggttcccaat ccgagatccg gcatcagcgc gatggcctgc tcgaccagct
     6841 cctcatattc gcgaacggca taacgccggt tcatcgcctg caggattcca tcgtcgccgc
     6901 tttgcagggg aaggtgcagg taatgacaca gtttcgtgga gccggccatg tgctccagca
     6961 aggcagcagg gacggtcgtg ggttcgatcg aggaaatcct gatccgggtg acgtcgggaa
     7021 tcgcgtcgag ttcccgcagg agttcaacca gtccgagacc ctgatatgag tattggccga
     7081 tgttcacccc ggtgagcacg agttcgcgat agccatgggc ggcgagctca cgggcttccc
     7141 gcaagacatc ctcggcggtc cggctgcgct cacgcccgcg cgcgaacgga atcaggcaaa
     7201 agctgcacat gaagtcgcag ccgtcctgaa tcttcagcag ggcgcgggtc gagtcggaat
     7261 atgcggtgcc gggcaggacg aagtcttcgc gatcgatcgt acgggtatgg cggagttcgg
     7321 gctccggctg tttcaggagt ttggccggcg ccgggagata gtcagggaga ttcattttga
     7381 actgggtgcc gacgatcagg tcgatgccgg gtaccttctg cagctgcgtg gcaccggtct
     7441 gggcataaca accggtcacc gcgacgaagg catgcggcga gtggcgcaag gtcttgcgga
     7501 cggcataacg gcaatctttt tcggcattct ccgtcaccga gcaggtgttc aagacgagca
     7561 ggtccgtctc cttgccgaac tccaccagtc ggtacccccg gcgtgccagg gtatcggcta
     7621 acatggaggt ctcggattgg ctgagacggc agcccaatgt atgaagtgac gcgcgtggca
     7681 tgctggtgga catcgtctgc gattataaga gcgcgcacct tcgatcggca aggtgacgag
     7741 gacccgaaaa agtcagccga gcgcttgaac ggggtatagc tgggtggtag catgagtgaa
     7801 attgtcccca tcatggcgtc gatctgcttt ctgaaccatg acgaaccttc gcgttgccgc
     7861 tagtcttctg ctcgctgtgc tcctctttat tccggctacc tccgcttggt cacaggatcc
     7921 gcttcctatc gagcccgacc tgaatagtcg cctcgatgaa ctgtacgacc atgaggcgcg
     7981 actgttcatc atgctctatt cgctacatgg cgacgggaag gtcgactata ttacgggtcg
     8041 tctggtgcag gagtatacga ggagcaatta cgggaatccg gtgtactaca ccgagccgta
     8101 ccccctgttt tattggtggg accacacgat gttcaacgat ccggatcagg acggggtgaa
     8161 cgggaacgag cgtgtctacc aggagaacat cgagttcgat atcgcgcgat a
//
LOCUS       DAOQRM010000108         8113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_8426_length_8113_cov_11.681062, whole genome shotgun sequence.
ACCESSION   DAOQRM010000108 DAOQRM010000000
VERSION     DAOQRM010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8426_length_8113_cov_11.681062"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="PKK81_09190"
     CDS             complement(<1..1267)
                     /locus_tag="PKK81_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNV33093.1"
                     /translation="MICGGLLGALFGQEPYWGGLRNAQLGKLGLFVVTLLKTLAIPLI
                     FFAILDALIRTTIPLRQGGKLLLICLVNVSVAMAIGLAIMNTWQPGLAWQGRVDALLQ
                     LVPGTKPSATVLANVQAGSQSPIEYLASYIPRTIADPFSSNNIIGVVLLALVLGATLR
                     MVRSEKDQTTGLVNALVRGIERVYVWLVQILEWIILVVPLAVFGVVAQVVGKAGIGVF
                     SVLWIFLVAMLAGLAAHALIYYPLLAWLVGKKSPKVYLGQGADAILTAVSCNSSLATV
                     PVTLQCLHRMNVSPQSSRLAACVGTNLNNDGITLYEAMAALFLAQALGFDLPMVKQVL
                     IVLASIIAGAGVAGIPEAGLIVLPLVLAAAGLPDHVIIAAIPLIMTVDWIIARARSGV
                     NVMSDMLVAILLDAGHVAPAAEPAAIPRPS"
     gene            1618..2802
                     /locus_tag="PKK81_09195"
     CDS             1618..2802
                     /locus_tag="PKK81_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNV33094.1"
                     /translation="MRRFRLLSLTLGLLLSCTSPALSELLALLNYESKPDQSVRREGI
                     AIMDIDPESSDFGKVLMEIPLPPDLVAHHIFFNRDRTKAYITALGKSVLHVVDLTRFP
                     YRLRAIDVPDCQVLEDLVVSEDNRIWYLTCMGSSAVIMGDAVKDKPVKTIRTPDGGGA
                     SVLYPHGIAIHNGIDRVLITSTVKPDMSDAGDSVTVLQASTGAVLSTHKVSSKPSPAK
                     AAPVEVMFAPNSNPPVVQITNMLEGTLWTGTWDAASQSFSFQQVDDYGPRQQGMPLEM
                     LYNAKGDRLFVTTAKPGFVNLYDNSDPSHPRFLQAIPAAQGAHHSVLSPDERYLFVQN
                     SLLNLEGMSDGSITVIDLSKGGTVLGSIDTLKAQGFNPNCIMLLPNHFRQANLRASLV
                     AK"
     gene            2984..3481
                     /gene="moaC"
                     /locus_tag="PKK81_09200"
     CDS             2984..3481
                     /gene="moaC"
                     /locus_tag="PKK81_09200"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNV33095.1"
                     /translation="MAEFTHFNESGRARMVDVGAKASTERLATAQATVFLQPETLEKL
                     RQGKIAKGDVLAVAQVAGVMGAKKTPDLIPMCHPILLTSVDISFTETAEPDQEGRCAI
                     TITATAKTTGPTGVEMEAMTAASVAALTIYDMCKAVDRGMSFGDICLLTKSGGKSGLY
                     TRKVG"
     gene            3483..>3679
                     /locus_tag="PKK81_09205"
     CDS             3483..>3679
                     /locus_tag="PKK81_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33096.1"
                     /translation="MVTIQLFGMTKMLAGNQGTLSLSLHDGKRVKDLVSVIDAAYPKI
                     GELLQKKKVLVSVNQEIAHEDL"
     assembly_gap    3680..3779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3780..4214
                     /locus_tag="PKK81_09210"
     CDS             <3780..4214
                     /locus_tag="PKK81_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNV33097.1"
                     /translation="TDDDAMLVRVQREDFSIDEELKRVRRRSKRIGGIAMFLGTARDR
                     SKGKDVDGITFEHYEGMAQKKLREIRERAIKDFGVIEVLVLHRYGEITIGENIVLIIA
                     AAEHRAEAFRACQWAIDELKQITPIWKLEHTPEGEVWVEEHP"
     gene            4268..5332
                     /gene="moaA"
                     /locus_tag="PKK81_09215"
     CDS             4268..5332
                     /gene="moaA"
                     /locus_tag="PKK81_09215"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNV33098.1"
                     /translation="MDHRTLDTPAPTIFDRLGRPLRSLRLSVTDRCNLRCKYCMPEDD
                     YAWLPRDTILTFEEMAELTAIFTELGVDKVRLTGGEPLLRRDLPRFVRQLSENRRIAE
                     IALTSNGVLMADQAADLSFAGLNRVTISLDTLRADRFRTLTKRDLHHQVFDGIKAVVQ
                     AGFPSLKFDTVIIKGYNDDELIDLIEYGKTVGGEVRFIEYMDVGGATDWSMNQVLSRA
                     EMLEHIGRHYGEVTPIVENSVAPAERFRLPDGTSFGIIPSTTTPFCRSCDRSRLTADG
                     MWYLCLYAKDGLDLRAPLRQGRSRDEIKSLITAAWEGRADRGAEERKALEALGLREQR
                     LIEINRLREDPHLEMHARGG"
     gene            5393..>6099
                     /locus_tag="PKK81_09220"
     CDS             5393..>6099
                     /locus_tag="PKK81_09220"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33099.1"
                     /translation="MFDPHTLTILSLGFVLGLRHALDADHLAALSTVLAERPTVQAST
                     AIGFFWGLGHTLMLLCVGTLLLALNLTIPETLASLFEFAVGVMLVVLGLSLARRIYRE
                     QWHLHTHEHDGQPHVHLHSHHLQPDHAHGHWYQGSLRPLLIGMAHGLAGSAALMLIVL
                     STVTGIGQGIGYIVVFGVGSILGMVGVGAILSLPVVYSVTVGPRAYWMVQGLACLASI
                     GLGLLMMVRIGLGGGWS"
     assembly_gap    6100..6199
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6464..7252
                     /locus_tag="PKK81_09225"
     CDS             6464..7252
                     /locus_tag="PKK81_09225"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNV33100.1"
                     /translation="MNVRVLGCHGSGQLIPGENGPIQCGTCGFLVNDQLLVDAGTIGS
                     RLFLDEQRRIRVVLLTHLHFDHIRELPTLADNLVGEIDEPVVIAAIPEVLDGLRAHIF
                     NGTVYPDFFRLPDPARPVFVPYELRAGREDLLCGMGVTPIRVNHVVPTVGFLLREGAH
                     RVLYSGDTYQTDALWQVAKEVTGLRAAFIESSFPNEMDELARVAKHLTPALLAQEFAK
                     MARPELPVYAYHLKPRFRDQIRQELGQLGIPHLTALEEGQTLVL"
     gene            7263..8099
                     /gene="accD"
                     /locus_tag="PKK81_09230"
     CDS             7263..8099
                     /gene="accD"
                     /locus_tag="PKK81_09230"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNV33101.1"
                     /translation="MAWFKKGKTEEAEPPKRSKVAEGMWLKCNHCREIVYRKEVDRNN
                     KVCPKCDYHFPISVIERINLLVDLGTFKEWDAELEPQDPLQFQDTRTYKDRIKAQQEK
                     TGRKDAMVIGQGAINGRKVALCVFDFGFMGGSMGSVVGEKICRAVDRALEGRMPLILV
                     TASGGARMQEGILSLMQMAKTSAAVAKLGEAKVPFISILADPTFGGVTASIAMLGDVI
                     IAEPKALIGFAGPRVIEQTIKQQLPDQFQRAEFLLDHGMIDMIVERKQLKEAVSTLVG
                     HF"
BASE COUNT         1555 a         2216 c         2580 g         1562 t
ORIGIN      
        1 cggacgggcg aggaattgcg gccggttcgg cggcaggcgc gacgtgcccg gcatcgagca
       61 ggatggcgac gagcatatcg ctcatgacgt tcacgccgga gcgggcgcgg gcgatgatcc
      121 aatccaccgt catgatgagt ggaatggccg caatgatgac gtgatccggc aggcccgcgg
      181 cggctagcac cagcggcagg acaatcagtc cggcttcggg aatgcccgcc acaccggccc
      241 ccgcaatgat ggaggcgagc acgatcagca cctgtttcac catcggcagg tcgaagccca
      301 gcgcttgcgc gagaaagagg gcggccatgg cttcatagag ggtgatgccg tcgttgttga
      361 ggttcgtacc gacgcaggct gcgaggcgcg acgattgcgg cgagacgttc atgcgatgga
      421 ggcactggag tgtcaccggc acggtcgcga ggctgctgtt gcaggacacg gcggtcagga
      481 tggcgtcggc cccctggccc aggtagacct ttggcgactt ctttcccacc agccaggcga
      541 ggagcggata atagatgagc gcgtgggccg cgagtcctgc gagcatggcg acgaggaaga
      601 tccagagcac ggaaaagacg ccgatgccgg ccttgcccac gacctgcgcc acgacgccga
      661 agacggcgag cggcaccacc aggatgatcc attcgagtat ctgcaccagc cagacataga
      721 cccgttcgat gccgcgcacg agggcattga ccagcccggt ggtctggtcc ttttcgctcc
      781 ggaccatccg cagggtggcg ccgagcacca gcgcgaggag gacgacgccg atgatgttgt
      841 tgctcgagaa cgggtcggcg atggtgcggg gaatgtagga ggcgaggtac tcgatcgggc
      901 tttgagaccc ggcctgcacg ttggccagca cggttgcgga gggcttggtc cccggcacca
      961 gttggagcag ggcatcgacg cggccctgcc aggccaggcc cggctgccag gtgttcatga
     1021 tcgcgaggcc gatggccatg gcgacggaga cgttcacgag gcagatcagc aggagtttac
     1081 ccccctggcg cagcggaatg gtggtgcgga tgagcgcatc gaggatcgcg aaaaagatca
     1141 acggaatggc gagtgtcttg agcagggtga cgacgaagag gccgagtttg cccagctgcg
     1201 cattgcgcag accgccccaa tagggctcct gtccgaagag ggcgcccagc agcccgccgc
     1261 agatcacggc aatgagcact tgtgtgtaga gcggcagtgg gatccagcga tgcgtcggct
     1321 tcatgtcgat ctctagtgga ggcggaagaa taccggtttc ttcactggat aacatcccgc
     1381 ttgggggaaa ggctagcccg gactcttcag gaggacctcc tcagtcgtct gctcctctcg
     1441 aaccgcgggc ttcagtcttg aactcctcgg taggacattg gtgaaaaatc ctggctgggc
     1501 cctaagggag tgggtctgaa acccggcgca ggcgatcagc gccagcgctg cccattgcga
     1561 ggaccttgtg aaaggagtat tttcttaagc attctcacct cacagaagga ggctattatg
     1621 agacgcttcc gactcctctc cttgaccctc gggctgcttc tttcctgtac atcgccggcc
     1681 ttgtcggaac tgctggccct gctcaactac gagagtaagc cagaccaatc ggtcagacgg
     1741 gaagggatcg cgattatgga catcgatccc gagtcgtcgg acttcggaaa ggtcttgatg
     1801 gagattcccc ttccgccgga ccttgtcgcg caccacattt tcttcaaccg cgaccggacg
     1861 aaagcctaca tcacggcctt aggcaaaagc gtcctccacg tcgtggacct cacccgcttt
     1921 ccctatcgcc tccgcgccat cgatgtgccc gactgccagg tgttggaaga tctcgtggtg
     1981 tcggaggata accggatctg gtacctgacc tgtatggggt cgagcgccgt catcatgggt
     2041 gatgctgtga aggacaagcc ggtcaagacc attcgtacgc cggacggcgg gggcgcgtcc
     2101 gtgctctatc cgcacggcat tgcgattcac aacgggatcg accgtgtgct gattacgagc
     2161 acggtgaagc ccgatatgtc ggatgcgggc gactcggtga cggtgttgca ggcgagtacg
     2221 ggtgcggtgc tctcgacgca caaagtgtcc tctaaacctt caccggccaa ggctgccccg
     2281 gtcgaagtga tgtttgcgcc caactcgaac cccccggtgg tgcagatcac aaacatgttg
     2341 gagggaacct tgtggaccgg cacctgggat gcggcgagcc agtcgttttc gtttcagcag
     2401 gtggacgact acgggccccg gcaacagggc atgccgctgg agatgctcta caatgcgaag
     2461 ggggatcgat tgttcgtcac gacggcgaag ccgggatttg tgaacctgta cgacaattcc
     2521 gaccccagtc acccgcggtt tctccaagcc atccccgcgg ctcagggcgc ccaccatagc
     2581 gtgctgtcgc ccgatgaacg ctacctcttt gtccaaaaca gcttgctcaa cctggaaggc
     2641 atgagcgacg ggtccatcac cgtgattgat ctgtccaaag gcgggacggt gctggggagt
     2701 atcgacacgt tgaaggcgca agggttcaac ccgaactgca tcatgctgct gccgaatcat
     2761 ttccggcagg ccaacttgcg cgcaagcctc gttgcaaagt gaaatggtgt gagggcacaa
     2821 cgtaggcaaa cgagacagcg aaggcggatg ttcagtgcga aattggagga aactccagca
     2881 tcctgttgcc cggctacgcc cgcttccctt cttgactccc attttcaccg gctcctataa
     2941 ttcggcggtt cacacgcggc aaccagctga tttggcgtag agaatggccg agtttacgca
     3001 tttcaacgag tcggggcggg cacggatggt cgatgtcggg gcgaaggcct ccaccgagcg
     3061 tctcgcgacg gcgcaagcca cggtattcct tcagcccgag accctcgaaa agctccggca
     3121 ggggaagatt gccaaggggg atgtgctggc cgtcgcccag gtggccgggg tgatgggggc
     3181 gaagaagacc ccggatttga tccccatgtg ccatcccatc cttttgacca gcgtcgatat
     3241 ttctttcacc gaaacagctg aacccgacca ggaagggcgt tgcgcgatca cgatcaccgc
     3301 cacggccaag accacgggtc cgaccggcgt ggaaatggaa gccatgaccg ccgcttcggt
     3361 ggccgcgttg acgatctacg atatgtgcaa ggcggtggac cggggcatga gcttcggcga
     3421 catctgcctg ttgacgaaat ccggcgggaa gtccggcctc tataccagga aggtcggctg
     3481 agatggtcac gatccaactt ttcggtatga ccaagatgct ggccggtaac caagggacgc
     3541 tgtctctgtc gctgcacgac gggaaacggg tgaaggacct ggtgtcggtg atcgatgcgg
     3601 cctatccgaa gatcggcgag ttgttgcaga agaaaaaggt gctggtgtcg gtcaatcaag
     3661 agatcgcaca cgaggatctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     3781 ccgacgatga cgcgatgctg gtccgcgtcc aacgggaaga cttttcgatc gacgaggaac
     3841 tcaagcgggt acgccggcgc tccaaacgga tcggtggcat tgccatgttt ctgggcacgg
     3901 cccgtgaccg ctcgaagggg aaggatgtgg atggcatcac cttcgagcat tacgagggca
     3961 tggcgcagaa gaaactccgc gagattcgtg agcgtgccat caaggatttc ggcgtgatcg
     4021 aagtgctggt cctgcaccgg tacggtgaga tcaccatcgg agagaatatt gtgctgatca
     4081 ttgccgcggc tgagcatcgc gccgaggcct tccgtgcctg tcagtgggcg atcgacgaac
     4141 tgaagcaaat tacgccgatt tggaaactcg agcacacccc cgaaggagag gtgtgggtcg
     4201 aggagcaccc atagcccctg tcaggggcga gcagtcaggg gggagaggcc cgcgccttcg
     4261 agtcactatg gaccaccgca cgctcgatac acccgctccc acaattttcg accgcttagg
     4321 gcgcccgctc cgcagtctgc gcctgtctgt gaccgaccgc tgcaaccttc ggtgcaagta
     4381 ttgtatgccc gaagacgact atgcctggtt gccgcgcgat accatcctga cctttgaaga
     4441 gatggccgaa ctcacggcga ttttcacgga gctgggggtg gacaaggtcc ggcttacggg
     4501 cggggagccg ctgttgcgtc gagacctgcc gcggtttgtc cggcagctgt ccgagaatcg
     4561 ccggattgcg gagatcgccc tcaccagcaa cggtgtgctc atggcggacc aggctgccga
     4621 cctgtcgttt gccgggttga atcgcgtcac gatcagtctg gataccctgc gggccgaccg
     4681 gttccgcacg ttgaccaagc gtgatcttca tcatcaggtg ttcgacggta tcaaggcggt
     4741 ggtgcaggcc gggtttcctt cgctgaaatt cgacacggtc atcatcaaag ggtacaacga
     4801 cgacgaactg atcgatctga tcgagtacgg aaaaacagtc ggcggcgagg tccggttcat
     4861 tgagtacatg gatgtgggag gggccacgga ttggtccatg aatcaggtac tttcgcgggc
     4921 ggagatgctg gagcacatcg gccgacacta tggcgaagtc acacccatcg tcgagaacag
     4981 tgttgcgccg gcggagcggt tccggttacc ggacggcacc agcttcggca tcattccgtc
     5041 caccaccacg cccttttgtc gctcctgcga tcggagccgc ctcacagcgg acggcatgtg
     5101 gtatctctgt ctgtatgcga aggacggcct cgacctgcgg gcgccgttgc gccagggccg
     5161 ctcccgcgac gagatcaaat cgttgatcac cgctgcctgg gaagggcggg cggatcgggg
     5221 tgccgaagag cgcaaggctc tcgaagcgct cggcctccgg gaacaacgac tcatcgaaat
     5281 caaccgtcta cgcgaagatc cccacctgga aatgcatgcg cgagggggct gaccgtcagt
     5341 tcatgctgtt cctgttaccg gggcagtcga ggcacttctc ccggagcagc ggatgttcga
     5401 tcctcatacc ctgaccatct tgagtctcgg attcgtgttg gggcttcgtc acgctctgga
     5461 cgcggatcat cttgccgcgc tctccacggt attggcggag cgtccgaccg tgcaggcttc
     5521 cacggccatc gggttttttt gggggctcgg gcacacgctg atgttgctgt gtgtcgggac
     5581 cctgctgctg gccttgaacc tcacaattcc tgaaaccctg gccagcctgt tcgagtttgc
     5641 cgtcggggtc atgctggtgg tgctcggcct ctcgctggca cgccggatct accgggaaca
     5701 atggcacctc catacgcatg aacacgatgg gcagccgcac gtgcatctac atagccacca
     5761 tctgcagccg gatcacgcgc acggacattg gtatcaggga tcgcttcggc cactcttgat
     5821 cgggatggct cacggactgg caggatcggc ggcgctgatg ttgatcgtgc tctcaaccgt
     5881 cacggggatc ggtcaaggca tcggctatat cgtggtgttc ggcgtggggt ctattctcgg
     5941 catggtcggc gtcggggcga tactgagtct gccggtggtc tattctgtca cggttggtcc
     6001 gcgcgcctat tggatggtgc agggcctggc ttgtctggca agtattggct tgggattgtt
     6061 gatgatggtc cggatcgggc tgggcggcgg ttggtcctan nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnt tggcttggga ttgttgatga tggtccggat cgggctgggc
     6241 ggcggttggt cctagccgat ctgtgaatgc cgacgagctg tcgcatttga gaacagattg
     6301 gggatgtacc cttcccgcgg cgagggggct catggaaccg accgtgcaaa tctgtgtgcg
     6361 ccttttcgag aggtatgtta ttctgccccg cagtgcggag gacacatcca caagcttctg
     6421 atggagcgcc ggagttcgag ggacgttccg ggaatccaca gccatgaatg tccgcgtgct
     6481 gggctgtcat ggctccgggc agctcattcc gggggagaac ggtccgatcc agtgcggtac
     6541 ctgcggtttt ctggtcaatg accagttgct cgtggatgcc ggcacgatcg ggtcgcgcct
     6601 gttcctcgat gagcaacggc ggattcgggt ggtcctgctg acccatctgc atttcgatca
     6661 tattcgtgaa ttgccgacgc tggccgataa tctggtcggc gagatcgatg agccggtggt
     6721 catcgcggcc attcccgaag tgttggacgg gctacgcgcg catattttta acggaacggt
     6781 gtaccctgat ttcttccggc tgcccgatcc cgccaggccg gtgttcgtcc cttacgagtt
     6841 gcgagcggga cgagaggatc tgctgtgcgg gatgggcgtg acgccgattc gcgtcaacca
     6901 tgtcgtgccg accgtgggat ttctccttcg cgaaggggcg cacagggtgc tctatagcgg
     6961 tgatacgtat caaacggacg cgctctggca ggtggcgaag gaagtcaccg ggttgcgggc
     7021 ggcgtttatc gagagttcgt ttcccaatga gatggatgag ttggcgcgtg tggcgaaaca
     7081 cctgactccg gcgctgctgg cacaggaatt tgccaagatg gcgaggccgg agttgccggt
     7141 ctacgcctac catctcaagc cgcggtttcg cgaccagatc cggcaggagc tcgggcaact
     7201 gggtattcct catttgaccg cgcttgaaga gggtcaaacc cttgtactat aggtgagcac
     7261 agatggcatg gtttaaaaaa ggcaaaaccg aggaggcaga gcctcccaaa cgctccaaag
     7321 tggcggaggg gatgtggctg aagtgtaatc actgccggga gatcgtgtat cgcaaagagg
     7381 tggaccggaa caacaaggtc tgtccgaagt gcgactatca ctttcccatt tcggtgattg
     7441 aacgtatcaa cctgttggtg gatctcggaa ccttcaaaga gtgggacgcc gagctggagc
     7501 cgcaagaccc gctccagttt caggataccc gcacgtataa agaccggatc aaggcgcaac
     7561 aggaaaagac cggccgtaaa gacgccatgg tcatcggcca gggggcgatc aacgggcgca
     7621 aggtggcgct gtgcgtcttc gatttcgggt tcatgggcgg cagtatgggg tcggtcgtcg
     7681 gtgaaaagat ctgtcgcgcc gtcgatcggg ccttggaagg gcgcatgccg ttgatcctcg
     7741 tgacggcttc gggcggcgcc cgcatgcagg agggcatcct gtcgctcatg cagatggcta
     7801 aaacgtccgc agcggtggcg aagttgggag aggcgaaggt gccgttcatc tccatcttgg
     7861 cggacccgac gttcggcgga gtgaccgcga gcattgcgat gctgggagac gtcattattg
     7921 ccgagccgaa ggcgctcatc gggtttgccg gtccgcgcgt catcgagcaa accatcaaac
     7981 agcaattgcc ggatcagttt caacgggccg agttcttgct ggatcacggc atgatcgaca
     8041 tgatcgtcga acggaaacag ttgaaggaag cggtcagtac actggtcggc cacttctagc
     8101 aggatgcgga aaa
//
LOCUS       DAOQRM010000109         8092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_8454_length_8092_cov_17.411099, whole genome shotgun sequence.
ACCESSION   DAOQRM010000109 DAOQRM010000000
VERSION     DAOQRM010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8454_length_8092_cov_17.411099"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PKK81_09235"
     CDS             complement(<1..911)
                     /locus_tag="PKK81_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV33102.1"
                     /translation="MGTPLRRMSPWGLCLLWLAGALFSCGQGAGSDPAESRPVLHYFT
                     WSDYVGPDIIQEFERRERVKVVIDTFSSNEELLAKLQSGATGYDVAVPSDFMVAIMIQ
                     QGLLSELDQQALPNASFLEPHLQALPFDPERRYSIPYLWGSVGIGYDSAVIPTPPDSW
                     AILWDPRYKGRISMLNDQREVFGAVLRSMGQSMNSTDPQVIEAAKERLLRQKPLVKTY
                     TSDHYDQLLASGEVVLAHAWGGPVARAMAERPSIRYVVPKEGATLWADCLVVLRTAPQ
                     KQLAMRFINYLMEPQVAARTSERLRFAS"
     gene            complement(912..1685)
                     /locus_tag="PKK81_09240"
     CDS             complement(912..1685)
                     /locus_tag="PKK81_09240"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV33103.1"
                     /translation="MRRGSTGLSLVSGLAMLFLYGPILVLVLYSFNAAHLSMAWRGTT
                     LKWYAALWHDEALLAATANSLLIAVISTIGATLLGGLMALGMERMPLRRQQIIEGGLV
                     LPLVIPEVMMGVALMLLFVMLKMPLGLTTVILGHIVFNIPLVTIMIRTRLRKLDPALR
                     EAAADLGADSWQVFRHVTLPLLRPAIWGAVLVAFTVSLDDFLVTFFTAGPGATTLPLK
                     VYSMIKSGVTPEINALSALLLLISMACVALSLHLQRREV"
     gene            complement(1682..2572)
                     /locus_tag="PKK81_09245"
     CDS             complement(1682..2572)
                     /locus_tag="PKK81_09245"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV33104.1"
                     /translation="MPSHDLSSPPAPGQSTCRSCWLAAPGLLWMGLFFLVPLALVFAI
                     SFASRGTYGGIVWEFTAANYLDLWHPLYGKILGQSLWYASLTTAICFVLGFPLAYMIA
                     RSPARWQPVLLLLVMLPFWTNFLVRTYAWMIVLRQDGLVNGLLRALGIVDAPLELLYT
                     PTAVVIGLVYGYLPFMVLPLYVAVERLDPRLVEAAWDLYASRWAIFTRVVVPLTMPGI
                     VGGCVLVFIPSIGSFITPDLLGGARSMMIGNLIQHEYLVVRDWPLGSAVSFVLMAIVM
                     AAVALYYRHAARTAGPLEGR"
     gene            complement(2575..3714)
                     /locus_tag="PKK81_09250"
     CDS             complement(2575..3714)
                     /locus_tag="PKK81_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV33105.1"
                     /translation="MATSSTPPFTASEQSLSSTLPTIDIRGIVRRHGAVTAVDRVSFQ
                     VRRGEFFSILGPSGAGKTSVLRMLAGFDDPDEGDLLIDGQSMLGVPPNRRPVNLVFQS
                     YALFPHLTVFENVAFGLKMRRVSAALIAEQVRQALAMVKLLGKEGRMPNQLSGGEQQR
                     VALARALVNKPAVVLLDEPLAALDQQLRQAMQVELKSIQEQAGLTCVCVTHHQEEAMM
                     MSDRIAVMHQGRMWQVGSPREVYERPCNLFVSRFLGVSNELTGTIGASVGEDRVFRPD
                     DAEQAPLTVRTASTEQAGRSATLSLRPEHIRMAHERPSTSDPVLSGRIEKVLFAGSEM
                     KYLVRVAPGRFWETRMTSGTSLPAFAAGDPVFLHWSLADARLFFE"
     gene            complement(3754..4032)
                     /locus_tag="PKK81_09255"
     CDS             complement(3754..4032)
                     /locus_tag="PKK81_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33106.1"
                     /translation="MKKQNARQAVVKPDIEAANPMMEMVWRLERLERQVQRLSELVRN
                     HAEDNDRLVRIVAEDRDVIRRELLRKHEHRVRVRKHLRDVSSKVDLEH"
     gene            4168..5406
                     /gene="glgC"
                     /locus_tag="PKK81_09260"
     CDS             4168..5406
                     /gene="glgC"
                     /locus_tag="PKK81_09260"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNV33107.1"
                     /translation="MAKIFTMVLAGGKGERLNPLTEQRAKPAVPFGGKYRIIDFTLSN
                     CLNSGLRQMAVLIQYKSHSLDRHIRTGWNILNPELGEYIVSVPPQQRISEEWYRGTAD
                     AVYQNLFLLDNDHPEFLLVLAGDHIYKMNYADMYNLLIEKQADAVVGAIETPLADANR
                     FGVIAVDKDRRILSFDEKPEKPMHIPGDPTQAFVSMGIYLFRTDVVREQLIRDAKEGT
                     KHDFGKNIIPRMIKEQRVYAFKFQDENKKAVKYWRDIGTLDAYWEANMDLVAVDPLFN
                     LYDQQWPIRTYQGQFPPAKFVFAQDFQGGRMGVALDSIVCGGCIISGGRVQNSVLSPN
                     VRVHDHADVRESVVMENVVIGEQARIRRAIIDKDVIIPPKTVIGFDPVADRQRFKVTD
                     SGLVVISKGMKLHAAVDSSG"
     gene            5381..7243
                     /locus_tag="PKK81_09265"
     CDS             5381..7243
                     /locus_tag="PKK81_09265"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNV33108.1"
                     /translation="MPPSIHPVDLVATLRQRNLTPAIIFLTSRRACDEAMQSFERSQV
                     TLPKTQQEQIARVLAQVTEQYPSIAEHPLIDTVQRIGVAAHHAGHLPSWKIAVEELMR
                     TGSLDAVFATTTLAAGVDFPARTVVVTQSSIRKARDFTDLTASEIQQLAGRAGRRGKD
                     LVGFAVITPSPYIDLTVLTKGLTGQPEAIHSQFVISYPMVLNLLKAHPREQIQSILAK
                     SFAQFQLNQRTAVLETKLDGLRVEMEPFGPRVCSDWITQWQVFDQARKRKKPRGLSAR
                     QESPDVAARLHFLTPGRVVGLIRGRGIILRQYRSKGQHAPMVTLLRDGGSLNECPASI
                     VTQVFDRTFDCEETSAYPWCTPQTLEQLTHHLLDLPPRLPILPILTHQHEEIPLDSSI
                     IQTLGDFACPTCPSRQACQRDFPRASRVRQEIQRHMKSIQALQTSLWHRFQERIDVLE
                     RFGYLNATAQLTADGEWARLIRIDHSLLITELIRAEAFGAIDPPLLAAVMASIAHDDD
                     RPGSFPRGSAGLVTLLFQVRKLAESLVPHEAPPMLRADVAALTERWVADQTLTWIGLA
                     RLTTMAEGDMFRLFARTIEFLSQLKTLKATHPSLAESADRAIAGMRRGVLEELP"
     gene            7326..>8092
                     /locus_tag="PKK81_09270"
     CDS             7326..>8092
                     /locus_tag="PKK81_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV33109.1"
                     /translation="MTTHELEQLLLQQPVSLLHRLARGRISRHFRSGKRKLIDLLLQA
                     SAENRPGLESDLAALIEEQSHRQQAVPVEQAPKPKVHPRPAAASTTPRPHRAEEHGHH
                     EPPVSLHEWLSGIGVPQAQPFVPDAWQVDALARLAETDVVVSVPTGSGKTYVAIEATK
                     RAMQENRTVIYTSPLKALSNTKFTEFSRLFGPGQVGILTGDRREHAQAPLLIMTTEIL
                     RNLLYDAAGGEIDVRLDTLGLVIMDESQYLADPERGVV"
BASE COUNT         1738 a         2659 c         2298 g         1397 t
ORIGIN      
        1 gaggcaaaac gcagccgctc cgacgtgcgc gccgcgacct gaggctccat taaatagttg
       61 ataaaccgca tggctaactg tttctggggc gccgtccgga gcaccaccag acagtcggcc
      121 cacaaggttg ccccttcctt cgggacaaca tagcgaatgg acggccgctc ggccatggcc
      181 cgcgccacgg gtccacccca ggcatgcgcc aggaccacct cccctgaagc caacaactga
      241 tcgtaatggt cgctcgtata ggtcttcacc aaaggtttct gccgcagcaa ccgctccttt
      301 gccgcctcga tcacctgcgg atccgtgctg ttcatcgatt gccccatgga acgcaacacc
      361 gcaccgaaca cttcccgctg gtcgttcaac atgctgatgc ggcctttgta acgcgggtcc
      421 cataggatcg cccaactgtc cggcggcgtc gggatcaccg cagaatcgta cccgatcccc
      481 acgctgcccc acagatacgg gatcgagtac cgccgctccg gatcgaaggg cagcgcttgt
      541 aaatgcggtt ccaagaacga cgcgttgggc agcgcctgct ggtccaattc actcagcagc
      601 ccctgctgga tcatgatggc caccatgaaa tccgacggca ccgccacgtc ataccccgtg
      661 gccccgctct gaagcttggc cagcaactct tcattgctgc tgaaggtatc gatcaccacc
      721 ttcacccgct cgcgccgttc gaactcctgg ataatgtccg gccccacata gtcggaccag
      781 gtgaagtaat gcaggaccgg tcgagactcg gcaggatcac tccctgcacc ttggccgcag
      841 gaaaacaacg cccctgccag ccaaagcaga cacagccccc aggggctcat tcgtcggaga
      901 ggagtcccca ctcagacctc ccggcgttga agatgaaggg agagggcgac acaggccatg
      961 gaaatgagca gcagcagcgc ggagagtgca ttgatctccg gcgtgacacc ggacttgatc
     1021 atcgaataca ccttcaacgg cagcgtcgtc gcaccgggac cggccgtgaa gaacgtcacc
     1081 agaaaatcat cgagcgagac cgtaaaggcc accagcaccg cgccccagat cgccggacgc
     1141 aagagcggca acgtgacatg ccggaagacc tgccaggagt cggcgcccag atcagccgcc
     1201 gcctcgcgca acgccggatc cagcttgcgc aaccgcgtgc ggatcataat ggtcaccagc
     1261 ggaatattga acacgatgtg tcccagaatg accgtagtga gaccgagcgg catcttgagc
     1321 atcacgaaaa gcagcatcag cgccacgccc atcatcacct caggaatgac gagcggcagc
     1381 accagcccgc cctcgatgat ctgctgccgg cgaagcggca tacgctccat gccgagtgcc
     1441 atcagcccgc ccagcaaagt cgcgccgatg gtcgagatca cggcaatgag caggctgttc
     1501 gcggtcgcag ccagcagcgc ctcgtcatgc cagagggcgg cataccactt gagggtcgtc
     1561 ccccgccagg ccatcgacag gtgggcggca ttgaacgaat agagcacgag cacaagaatg
     1621 gggccgtaga gaaacagcat cgccagaccg ctcacgaggc tcaggccggt cgatcctcgc
     1681 ctcatcgtcc ctccaacggc ccggcagtcc gggcggcatg gcggtaatac agcgccaccg
     1741 cggccatgac gatcgccatc agcacaaagg acaccgccga tcccaacggc cagtcccgca
     1801 cgaccagata ctcgtgttga atgagattgc cgatcatcat gcttctcgcc ccgcctagca
     1861 gatccggcgt gataaacgag ccgatcgacg ggatgaagac caggacacag ccgccgacga
     1921 tgcccggcat cgtgagagga accaccaccc tcgtgaatat cgcccagcgc gaggcgtaca
     1981 gatcccaggc cgcctccacc aggcgcggat ccagccgttc cacggcgaca tagagaggca
     2041 gtaccataaa gggaaggtac ccgtagacca gtccgatcac gacggccgtc ggcgtgtaga
     2101 gcaattcaag cggggcgtcc actattccga gagcccgcaa caggccgttc acaaggccgt
     2161 cctggcgcag cacaatcatc caggcgtagg ttcgcacgag aaagttcgtc cagaacggta
     2221 acatcaccag aagcaacaac accggctgcc accgggccgg acttctggcg atcatgtagg
     2281 cgagcggaaa tccgagcacg aaacagatcg ccgtggtgag actcgcgtac cagagcgact
     2341 gcccgaggat cttgccgtac agcggatgcc acaggtccag atagttcgcc gccgtgaact
     2401 cccacacaat gccgccatag gttccgcgtg aagcgaagct gatcgcaaac accaaggcca
     2461 gcggcacgag aaaaaacagg cccatccaca acagaccggg agcggccagc caacaggaac
     2521 ggcaggtgga ttgaccaggc gcaggaggtg aagacaggtc gtgagagggc atggtcactc
     2581 gaagaacaac cgcgcatcag ccagggacca gtgaagaaac accgggtctc ccgccgcgaa
     2641 cgccgggagc gatgtgcccg aggtcatcct cgtttcccag aagcgaccgg gagcgactcg
     2701 aaccaaatac ttcatctcgc tcccggcaaa caggactttc tcaatccggc cggagagcac
     2761 cgggtcggat gtcgacggcc gttcatgcgc catgcggatg tgctccggcc ggagcgagag
     2821 cgtggctgaa cggccggcct gttccgtgga agcggttcgc accgtgagcg gcgcctgttc
     2881 ggcatcgtcc ggccgaaaca cgcgatcctc cccgaccgag gccccgatcg tacccgttag
     2941 ctcattcgac acgccaagaa atcgggacac aaacagatta cagggtcgct catacacctc
     3001 gcgcgggctt ccgacctgcc acatgcgccc ctgatgcatg acggcaatgc ggtccgacat
     3061 catcatcgcc tcttcctgat gatgcgtcac acagacacag gtcagcccgg cctgctcctg
     3121 aatggatttc agctcaacct gcatggcctg ccgcagttgt tgatccaacg cggctaacgg
     3181 ttcatcgagc agcaccacag ccggcttgtt caccagcgca cgggccaggg ccacacgctg
     3241 ttgttcgcca cccgataatt gattcggcat ccgtccttct ttgccgagca gcttcaccat
     3301 cgccaaggcc tggcgtacct gctcagcaat gagtgctgcg gacacccgcc gcatcttcag
     3361 gccgaaggcc acattctcga agaccgtcag gtggggaaac agcgcgtagg attgaaagac
     3421 cagattcacc ggccggcgat tcggcggcac gccgagcatc gactgcccgt cgatcaacag
     3481 atcgccttcg tccggatcgt cgaagcccgc cagcatgcgc aagaccgaag ttttccccgc
     3541 gccgctcgga ccaagaatgg aaaaaaactc gccccggcgg acctggaaac tcacccgatc
     3601 cacggccgtg accgcaccgt ggcggcgcac gatcccgcgt atatcgatcg ttggaagtgt
     3661 tgaggaaaga gactgttcag aagcggtgaa cggaggagtg gacgacgtgg ccatcgaagg
     3721 ctgaattcaa accgccgcgc cgacagccac ggctcaatgc tccaaatcga ctttggaact
     3781 gacatcgcgc aggtgtttgc gcacccgcac ccgatgctca tgtttccgca agagttcacg
     3841 gcgaatgacg tcgcgatcct ccgccacaat gcgcacgagc cggtcgttat cttcggcatg
     3901 gttccgcacg agctcggaga ggcgctgcac ctgccgctcc agccgttcca gccgccacac
     3961 catttccatc atcggattcg cggcttcgat atcgggcttg acgaccgcct gacgggcatt
     4021 ctgtttcttc atgtccgctc cttcgcattg agcccgaccg gatgtcggag ccgcacgttg
     4081 agtcgtagta tcgtccgaga tcgagttgaa gtcaatcatt ctgcatcctt tctattcatc
     4141 cgtcgtcctg ctacaatcgg cccgtctatg gctaagatct tcaccatggt gctggcgggc
     4201 ggcaagggcg aacgcctgaa tccgcttacc gaacaacgtg caaaaccggc ggtgccgttc
     4261 ggcgggaaat atcgcatcat cgatttcacg ctgagcaact gcctgaactc cggcctgcgg
     4321 cagatggccg tcctgatcca atacaaatcg cattctctcg accgtcatat ccgcacgggg
     4381 tggaacattc tcaatcccga gctgggcgaa tacatcgtgt ctgttcctcc tcagcagcgt
     4441 atcagcgaag aatggtatcg cggcaccgcg gatgccgtgt atcaaaacct cttcctgctc
     4501 gataacgacc accccgagtt cctactcgtg ctggccggtg accacatcta caagatgaac
     4561 tatgccgaca tgtacaacct gctgatcgaa aagcaggcgg atgcggtggt cggcgcgatt
     4621 gaaacgccgc tggcggatgc caaccggttc ggcgtcattg cggtggataa agaccggcgg
     4681 atcctgagct tcgacgaaaa gccggagaag ccgatgcaca tccccggcga cccgacccag
     4741 gcgttcgtct cgatggggat ctacctgttt cgcacggacg tcgtacgcga gcaactgatc
     4801 cgggacgcca aagagggcac caagcacgac ttcggcaaga acatcattcc gcgcatgatc
     4861 aaagagcagc gcgtctatgc gttcaagttc caggacgaaa acaaaaaagc cgtgaagtac
     4921 tggcgcgaca tcgggacgct cgatgcctac tgggaagcca acatggatct cgtggccgtc
     4981 gatccgctgt tcaacctgta cgatcagcaa tggccgatcc gcacctacca gggacaattc
     5041 ccgcccgcca agttcgtctt cgcccaggat tttcagggag gccgcatggg cgtggcgctg
     5101 gactccatcg tctgcggcgg ctgcatcatt tccggcggcc gcgtgcagaa ctcggtgctc
     5161 tcgccgaacg tccgggtcca cgaccatgcc gacgtgcgcg agtccgtcgt catggagaac
     5221 gtggtcatcg gcgaacaggc ccgcatcagg cgggcgatca tcgacaaaga cgtgatcatc
     5281 ccgcccaaaa ccgtgatcgg cttcgacccg gttgcggatc gtcaacgttt caaagtcacc
     5341 gattccggat tggtggtcat ctcaaaggga atgaaactgc atgccgccgt cgattcatcc
     5401 ggttgatctg gtcgcaacgc tccgccaaag aaatctcact ccggcgatta tctttctcac
     5461 atcgagacgg gcctgcgacg aagcgatgca gtcgttcgag cgcagccagg tcaccctgcc
     5521 caagacacag caggaacaga tcgcccgcgt cctggcccag gtcaccgaac aatatcccag
     5581 catcgcagaa cacccgttga tcgacacggt tcaacggatc ggtgtggcgg cgcatcacgc
     5641 gggccatctc ccctcctgga agatcgccgt cgaagaactc atgcgcaccg ggtctctgga
     5701 tgccgtcttc gccaccacca ccctcgccgc cggcgtcgac tttcccgccc gcacggtggt
     5761 cgtgacacaa tccagcatca ggaaggcgcg cgactttacc gacttgaccg ccagcgaaat
     5821 ccagcagctg gccggccggg ccggccgccg cggcaaagac ctggtcggat tcgccgtcat
     5881 caccccgtcg ccctatatcg atctcaccgt cctgaccaaa gggctcacgg gacaaccgga
     5941 agcgattcat agccaattcg tcatcagcta cccgatggtg ctgaacctgc tcaaagcgca
     6001 cccgcgcgaa cagatccaat ccattcttgc gaaaagcttc gcccagtttc agttgaatca
     6061 gcgcacggcc gtgctcgaaa ccaaactcga tggactgcgc gtcgagatgg aacccttcgg
     6121 tccgcgcgtc tgctcggatt ggatcacgca gtggcaggta ttcgatcagg ccaggaaacg
     6181 gaagaaaccg cgtggcctgt cggccaggca ggaatcgccc gatgtcgccg cgcgcctgca
     6241 ttttctcacg ccgggacgcg tcgtggggct gatccgggga cgcggcatca tcctgcgcca
     6301 gtatcgaagc aagggccagc atgcgccgat ggtgacgttg ctgcgggacg gcggttccct
     6361 caatgaatgc cccgcctcga tcgtgacgca ggtgttcgat cgcacgttcg actgcgagga
     6421 aacctccgcc tatccctggt gtactccgca gacactggag cagctgacac accacctgct
     6481 ggatctgccg ccgcgtctcc cgattctgcc gattctgact catcagcacg aagagattcc
     6541 gctcgacagt tcgatcatcc aaacccttgg cgatttcgcc tgccccacct gtccctcgcg
     6601 tcaggcctgt caacgcgatt tccccagggc ctcacgggtc cgccaggaaa tccagcggca
     6661 catgaaatcg attcaagcgc tccagaccag cttgtggcac cggtttcaag agcgcatcga
     6721 tgtcctcgag cgcttcggct atctcaacgc cacggcacag ctcaccgccg acggcgagtg
     6781 ggcgcgcttg attcgcatcg accattccct cctgattacg gaactgatcc gggccgaggc
     6841 cttcggcgcc atcgatccgc cgctgctcgc ggccgtcatg gccagtatcg cgcatgacga
     6901 cgatcgtccc ggctcatttc cacgcggcag cgcggggctc gtcacgctcc tgttccaggt
     6961 ccggaagctg gcagaaagtc ttgtgccgca cgaggcgccg ccgatgcttc gtgccgatgt
     7021 cgccgccttg accgaacgct gggtcgcaga ccagacgctg acctggatcg gcctggcccg
     7081 cctgaccacg atggccgagg gcgacatgtt ccggctgttc gcgcgcacga tcgagtttct
     7141 ctctcaattg aagaccctca aagcgaccca cccctcgctg gccgaatccg ccgatcgagc
     7201 catcgcaggc atgcgccggg gcgtgctgga ggaactaccg tgagcgtgtc gttcgcaaga
     7261 ggatttccga cgggcctcgt attcacacgt ttcacgtttc acgtttcacg catcacgagg
     7321 cctctatgac gacgcacgaa ctcgaacagc tactgctgca gcaaccggtc tctctgctgc
     7381 accgattggc gcgtggacgg atcagccggc attttcgttc cggcaaacgc aagctcatcg
     7441 atctgttgct ccaggcttcc gctgaaaatc gccccgggct ggagtccgac ctggctgccc
     7501 tgatcgagga acaatcacac agacagcagg ctgtgccggt tgagcaagct cccaaaccga
     7561 aggtccaccc gagaccggct gcagcgtcta cgacgccacg cccgcatcgg gccgaagaac
     7621 atggccacca tgagccgcct gtctcgcttc atgaatggtt gtcgggaatc ggagttccgc
     7681 aggcacagcc gtttgtaccg gatgcctggc aggtggatgc gcttgcgcgg ctggctgaaa
     7741 ccgacgtggt ggtcagcgtg ccgaccggca gcggaaaaac ctatgtcgcc atcgaggcca
     7801 cgaagcgggc gatgcaggag aatcgcacgg tgatttacac gtctccgttg aaagcgctct
     7861 ccaatacgaa atttaccgaa ttttcgcgcc tattcggccc ggggcaggtc gggatcctca
     7921 ccggagaccg ccgcgaacat gcccaggcgc ccctgctcat catgaccacc gaaattttgc
     7981 gcaatctgct ctacgatgcg gcggggggcg aaatcgacgt gcgcctggat acgttggggc
     8041 tggtgatcat ggacgaatcc caatacctcg ccgatccgga acgcggggtg gt
//
LOCUS       DAOQRM010000110         8085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_8469_length_8085_cov_15.588917, whole genome shotgun sequence.
ACCESSION   DAOQRM010000110 DAOQRM010000000
VERSION     DAOQRM010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8469_length_8085_cov_15.588917"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..950
                     /locus_tag="PKK81_09275"
     CDS             294..950
                     /locus_tag="PKK81_09275"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNV33110.1"
                     /translation="MNIRGRFIGFLIVAIAALAAVFFGHLFLFEQWRVQQERQLHRSK
                     ILGNVAHLQRLVLEVETNFRGYLLTEQQSFLEPIRLAENRLKTGLAQLTDLTATTPGL
                     QAGVSVLSARLDEFVESKQRLLAVIGAEQQDLVRSYVRGGSGRALFLTIEKAIGDFEV
                     RIERELPSESMTYDSWLQRARWQLLLVESLGIMVCVFLTRAALLPNRTVLAPNPRPLL
                     "
     gene            complement(1279..1641)
                     /locus_tag="PKK81_09280"
     CDS             complement(1279..1641)
                     /locus_tag="PKK81_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNV33111.1"
                     /translation="MIRIKRVYETPNARDGIRILVDRIWPRGCTKEQARLDAWRKEVA
                     PSTALRTWFGHDPAKWMEFQTRYRKELSRPEQNRAIEDLARLAQAQTVTLLYGASDKE
                     HNQAVVLKECIDSAKNRV"
     gene            complement(1669..2094)
                     /locus_tag="PKK81_09285"
     CDS             complement(1669..2094)
                     /locus_tag="PKK81_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV33112.1"
                     /translation="MSRVGIPADGFKTVGQIVGTNTIQFHTVQDGMTITIELLSAHVS
                     GGPVLDAEGRYVGFISEFDILKALEAGKDLNSLTATELMAKDPIAVRKSTPIPDAIKL
                     MADNHLLNLPVEENGEVTYSVTRHDLLRASVGLAVGIEE"
     gene            2484..2834
                     /locus_tag="PKK81_09290"
     CDS             2484..2834
                     /locus_tag="PKK81_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33113.1"
                     /translation="MDPQVVSPGDGAIDEQILGLLQHHRRLTFLTLAESLPLYTWQAL
                     FAALNRLRGQRHVDLLPLAADYEVVWQQGSERPSLMSEGPSRCDGDVAMDRTARRVRV
                     IEGGKGCPAVDVGV"
     gene            3130..3570
                     /locus_tag="PKK81_09295"
     CDS             3130..3570
                     /locus_tag="PKK81_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV33114.1"
                     /translation="MEGYGKRVLIAEDEESVRRLLAVVLEEAGYTVHAVEDGMEALAE
                     MKKRRFDAVVADYRMPRLDGEQFLLLSRLMWPQTPTVLLSAEHTDLPVILKMQGACAL
                     VPKPFDSQGLLRTLQSAVAAACSCCSPSSIPREGTNNTLSVAPS"
     gene            3747..3992
                     /locus_tag="PKK81_09300"
     CDS             3747..3992
                     /locus_tag="PKK81_09300"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNV33115.1"
                     /translation="MKKKTAPKKAASRPAKRMTSVTKETKAPGPVQDIQVRIAARAHE
                     LYEQRGCLDGYHLHDWLEAEREILGHPVVMDKSSERI"
     gene            complement(4261..6078)
                     /locus_tag="PKK81_09305"
     CDS             complement(4261..6078)
                     /locus_tag="PKK81_09305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNV33116.1"
                     /translation="MAVSRKIAAATSSRRSSASREFADHWELSDLVTDPVKQFDRYLK
                     DLSAKVSRFESARAELSATMSEQAFLNLLTLSEQLARDSGRLGAYAYLWFSEDTKQLQ
                     ARSFKTKVEEHLTALQNHLLFFDLWWQSVDQRNAERLMANSGDFRYHLETIRRFKPHT
                     LSEPEEKIINIKNITGQSAVHQLYDVVTNGFTFTMRIGGKKKTMNREALTAYLRSPKA
                     AVREAAYREMYRVYESQQDLLGEIYKTLVNDWKAENLQLRHFKTPLESRNLSNDIPDR
                     AVDALLSVCMKNAPIFQRYFKLKARLCKIKTMNRYHIYAPHRAEQKTYRYADAVRMVL
                     DAYYGFSPRLAELAQRVFADRHIDARTKPGKMGGAYCYSVTPNLTPYVMLNFTGEARD
                     IATMAHELGHAVHAMMAEQHNVFTFHSTLPLAETASVFGERILSDALMATETNRSVKQ
                     SLLVNQLDDIYATVMRQAYFVAFERTAHDMVAQGATTTDLAHTYMTLLRQQFGKSLRV
                     PQEFQWEWLTIPHLYASPFYCYAYSFGNLLVLALYRMYQEQGSGFVPKYLELLAAGGS
                     KAPQAILAEVGVDMNSPAFWQSGFDAISGMVDQLEQTMN"
     gene            6231..6560
                     /locus_tag="PKK81_09310"
     CDS             6231..6560
                     /locus_tag="PKK81_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33117.1"
                     /translation="MTEQEINRAIQYVTAATSYGRDTVAEVLRTGLSELAQLSTQSTC
                     RFEREDLMGYLCLWTMKRTGHPEPLVREVLDGAGRWLDEVATTVAQRREAQADGGDDD
                     EPGAATA"
     gene            complement(6698..7015)
                     /locus_tag="PKK81_09315"
     CDS             complement(6698..7015)
                     /locus_tag="PKK81_09315"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV33118.1"
                     /translation="MPNPRIEPLKRVLALEPDDDVAWFGLGKAYMEDANFEEAAKALR
                     QCITVKPTYSAAYYALAQSLQKLNRVEECRQVSDQGIEVSTKNGDAMVTKNLEALKSA
                     LPA"
     gene            7161..>8085
                     /locus_tag="PKK81_09320"
     CDS             7161..>8085
                     /locus_tag="PKK81_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNV33119.1"
                     /translation="MGTTGLIYDPRYLEHDMGAGHPESPNRLRAIMQRLEQSGTLATL
                     TRIDPRTAEDEWVTLVHRSEYVAMLNRQAPTHGRISLDADTSMSPGSLGAAYLAAGGA
                     LAGVDAIMAGRVQHAFCAVRPPGHHAEADRAMGFCLFNNVAIAARYAQKRYGVQRVLI
                     VDWDVHHGNGTQHSFESDASVLFFSTHQYPHYPGTGRASECGRGAGEGLTINVPMEAG
                     EGDDEYRAVFQKVLVPAADAFKPEFVIISAGFDAHRDDPLASMGLTEEGYADLTNIVA
                     GIARQHCQGRLLSALEGGYNLTALAASVERHI"
BASE COUNT         1578 a         2163 c         2582 g         1762 t
ORIGIN      
        1 caggtcgaag agccgatcta cattcggccg gccgagcgcg tgaactggct gtaagtcgag
       61 ggtcgcgcac ggatgcaacc atggcgttgc gctggggcct gtgccgccga tcctgtgaga
      121 ggaaggcctg cgggtagtct tcgtgaggga gcgatccttc acggggactg cccccgcctg
      181 ttactggccg atgatgccat gcagcgtggg cgaagtccgg gcgaaggccg aaggggcgcc
      241 atcacgtttc gatccgctga ttgtctgagg cttggaatgc gtgacagaga gtgatgaata
      301 tccggggacg gttcataggg ttcctcatcg tcgccatagc tgcgctggcc gcggtgttct
      361 tcggccacct gttcctgttt gagcaatggc gggtacagca ggagcggcag ctccaccgtt
      421 ccaaaattct cggcaacgtg gcgcatttgc aacggttggt cttggaggtg gaaacgaact
      481 ttcgcggcta tctgctcacg gagcagcagt cgtttctcga accgattcgg ctggcggaga
      541 atcgattgaa aacaggactg gctcaattga ccgatttgac cgccaccact ccgggtttgc
      601 aggccggagt gagtgtgctt tcggcccggc ttgatgaatt cgtggagagc aaacagagat
      661 tgctggcggt catcggggcg gagcagcagg acctcgtccg ctcgtatgtg cggggaggga
      721 gtggtcgggc gttgttcctg accatcgaaa aagccatcgg ggattttgaa gtgcgcattg
      781 agcgcgaact gccgagtgaa tccatgacct atgactcgtg gctgcaacga gcacgctggc
      841 agctgttgtt ggtggaaagt ctggggatca tggtgtgtgt cttcctgacg agagcggcgc
      901 ttcttccgaa taggaccgtg ctggcgccga accctcgacc tcttctgtga gtctatccgg
      961 gctatcgagt cgttcctccc ctcgattcag ttacctgtcg cgatctacgc cccgtatcgc
     1021 tctccatgtg tcgattcgca cagtgcgtag cgtgcgaatt cgctcgattt gaggctggga
     1081 aaatctgaaa accattggaa ttccgttcat gagtaatggc atgtgccttg ctttaaggac
     1141 gttcatcaca gtgaacgcat acgaaacaag gagtcagccc atggtacgga tgatcaagaa
     1201 agtcggagat cgggagttgg cctgggtgat gttggcgatt gccgcggtcg ctctctggag
     1261 cacctggggg ttggcgagct aaacgcgatt cttcgcactg tcgatgcatt ccttcaggac
     1321 cacggcctga ttatgttcct tgtctgatgc cccatagagc agtgtgacgg tctgtgcttg
     1381 tgcgagccgg gcgagatctt cgatcgcgcg gttctgttcc ggtcggctca gttccttgcg
     1441 gtaacgtgtc tgaaactcca tccactttgc cgggtcatgg ccgaaccaag tgcggagcgc
     1501 cgtgctgggc gccacttcct ttcgccatgc atcgaggcgc gcctgctcct tcgtgcatcc
     1561 cctcggccat atccggtcga ccagaatccg aatcccatcg cgcgcgtttg gtgtttcgta
     1621 gacccgtttg attcgaatca tgattgcaca gcaacaagtc gcgcgcggct actcttcgat
     1681 gcctacggca agaccgactg atgcacggag caggtcgtgc ctggtcaccg aataggtcac
     1741 ctctccgttc tcctcgacag ggaggttcag caagtggttg tctgccatga gcttgatggc
     1801 atccggtatc ggtgtcgatt tacggacggc aatgggatcc ttggccatca gctctgtcgc
     1861 cgtcagactg ttcaagtcct ttcctgcctc aagggccttg aggatatcaa attcactgat
     1921 gaatcccaca tagcggccct ctgcatccaa caccggtccg ccggacacat gcgcggaaag
     1981 caactcgatg gtgatcgtca tgccatcctg tacggtatgg aattgtatcg tattggttcc
     2041 gacgatttgt ccgacggttt tgaaaccgtc agctggaatt cctacgcgac tcatgatgtc
     2101 tcctttcgat gcggcaacag ccatcgaggg tgcgagatgt gtggcaggtc gatgaactgc
     2161 ggccagcgct gttcctgcga attcgtgttc atcagctcag caagtgaagt gcccgtccgg
     2221 atgggttgcg atttcctgaa ttcctgcgcc gttgcaggcg atgatcctgt gggtgcgagc
     2281 gattccgcac gggacagaca gtgcggaatg tcccggttaa gggcagctgg atgcggagtg
     2341 agcatgccgt aaccattgct gaatggcgga ttccgttcgc tggatgtgtg ccgtcagcag
     2401 gatcgcatat tgcaacgtct ccggggatgg gctggaccac acgtgaagcg agtagtccgt
     2461 tctgtccaca atggaggtgc gtgatggacc ctcaggttgt ttctccgggc gatggcgcca
     2521 tcgacgagca gattctcggt ttgctgcagc accaccggcg gctgaccttc ctcaccctgg
     2581 ccgaatccct tccattgtac acctggcagg cattgtttgc ggcgttgaac cggctccgag
     2641 gccaacgcca tgtggacctg ctaccgctgg ccgcagatta cgaagtggtc tggcagcagg
     2701 ggagcgaacg cccgtccctg atgtccgaag ggccctcaag atgtgatggc gatgttgcga
     2761 tggacaggac cgcacggcgg gtgcgagtga tcgaaggagg caaaggatgt cctgcagtcg
     2821 atgtcggggt ctgatggtgg ccgaaaacct gtttagtccc agcgaaggag tgtcgcatac
     2881 gtgggtgtcg gtcacgcgtt gcctcaattg cggcaatgtg gaagatccgt taatccgcct
     2941 ggcacgacgc cttccggaac aacttcatcg aagggttcgc cctggtccgc aacgcagagg
     3001 catatggatt cgtcggttat ggaaaccgga accagagtag ctatgccgtt tgggcggacg
     3061 ggattatggt cgtgacggaa ggaatgaggg tattccgtcg ttggaatgca gtcggaggga
     3121 ggaggtgcca tggaagggta tggaaaacgt gtgttgattg cggaggacga ggagagtgtg
     3181 cggcgcctgc tggccgtggt gctcgaggaa gccgggtata ccgtgcatgc ggtggaagat
     3241 ggcatggaag ccctggccga aatgaagaag cggcgtttcg atgcggtggt cgcggattac
     3301 cgcatgcccc gcctggatgg ggagcagttc ctgctgctga gtcgcctgat gtggccccag
     3361 actccgaccg tgctcctgtc cgccgagcat acggatttgc ccgtgatcct gaaaatgcaa
     3421 ggcgcctgtg ctctcgtgcc gaaacccttc gattcgcagg ggctgttacg gacgttgcag
     3481 tctgccgtag cggcggcttg tagttgttgc tccccgagtt cgatcccgcg tgaggggacg
     3541 aataacactc tctcagtagc gccttcctag cgggtggtca cctcgaggaa ggtgtctgtg
     3601 cttgaaggag ggagacgggg ttgaggggca cgttctgccg cctgatcaga tccggcctga
     3661 atatggtatc gtaggaacag atctctcgcg aaaagtatgg tgagaggcgg gcctgatgac
     3721 tggatacaag tgtagatgga gaatgcatga agaaaaagac ggcgccgaag aaggccgcct
     3781 cgcgtccggc gaaacggatg acgtcagtga ccaaagaaac caaggctccc ggtccggtcc
     3841 aggatatcca ggtacggatt gcggcacggg cgcatgaact gtatgaacaa cggggttgtt
     3901 tggatggtta tcacctgcat gactggctgg aagcagagcg ggaaatcctg ggacacccgg
     3961 tggtgatgga taagtcctca gagcgaatct gactagcccg cattattcat gagcgcttct
     4021 gctgcaatcg cggattcgca gctacgacca gcctgtcggc gggcgggctc gccgctcggt
     4081 cgatcgacat cctgttcaag gatgccttcc tccctcccgg ctccgcgcgc ccgtctcgct
     4141 ttgcgactcc gcaatttcgc gacgaaccgt catgaataag gcgggctaga tgcgtgaata
     4201 atcggctgcc aatgttcgga gagtccgcgc cgcccttgaa gtcagggtgg cgccgtccga
     4261 ttagttcatc gtctgttcca actgatcgac cattcctgaa atcgcatcga atccggattg
     4321 ccagaaggcg ggggagttca tgtcgactcc cacctccgca aggatcgctt gcggcgcctt
     4381 cgacccgcct gctgcgagta gctccagata tttcggcacg aacccgctgc cttgttcctg
     4441 atacatgcga tagagcgcca ggacgagcag gttcccgaaa ctatacgcat aacaataaaa
     4501 cgggctggcg tagaggtgcg ggattgtgag ccattcccat tggaattcct gcggcacccg
     4561 cagggacttt ccgaattgct gccggagcag ggtcatgtag gtgtgtgcca ggtcagtcgt
     4621 cgtggcgcct tgggccacca tgtcatgggc ggttcgttcg aaggcgacaa aataggcttg
     4681 ccgcatgacc gtggcataga tgtcgtccag ttggttgacg agcaagctct gcttgacgga
     4741 tcgattggtt tcggtggcca tcagggcatc ggagagaatc cgttcgccga agaccgaggc
     4801 cgtttccgcc agcggcaacg tggagtggaa ggtgaagaca ttgtgctgct cggccatcat
     4861 tgcgtggacg gcatgaccta actcatgcgc catggtggcg atgtcccgcg cttcaccggt
     4921 gaaattcaac atgacgtagg gtgtcaggtt gggggtcaca ctgtaacaat aggcgccgcc
     4981 cattttcccc ggcttcgtgc gggcatcgat gtggcgatcg gcgaagacgc gttgcgccaa
     5041 ttcggcgagg cgcggcgaaa agccgtagta ggcgtccagc accatccgga ccgcatccgc
     5101 gtagcggtag gtcttttgtt cggcccggtg tggcgcatag atgtggtagc ggttcatggt
     5161 tttgatcttg caaagtcgcg ccttcaattt gaaataccgt tggaagatcg gcgcattctt
     5221 catgcagacg gagaggagcg catccacggc gcgatccgga atgtcgttgc tgagattgcg
     5281 cgactccagc ggcgtcttga agtgccgcag ctgaagattt tccgctttcc agtcgttcac
     5341 cagggtttta tagatttccc ccaggagatc ctgctgagac tcatagacgc gatacatctc
     5401 ccgataggcg gcttcccgga cggcggcttt cgggctacgg aggtaggccg tcagggcttc
     5461 gcgattcatc gttttctttt tcccgccgat gcgcatcgtg aaggtgaagc cgttcgtgac
     5521 gacatcgtag agctggtgga cggcgctttg tccggtgatg ttcttgatgt tgatgatctt
     5581 ctcttccggt tccgagagcg tatgcggctt gaaacggcgg atggtctcca ggtggtaccg
     5641 gaagtcgccg ctgttcgcca tgaggcgctc cgcattccgc tgatccacac tctgccacca
     5701 caaatcgaag aacagcagat ggttttggag ggcggtgaga tgttcttcca ctttggtttt
     5761 gaaggaccgg gcctggagct gttttgtatc ttcggagaac cacaagtacg cgtaggctcc
     5821 gagccggccc gaatcccttg caagctgttc cgagagggtg aggagattga ggaacgcctg
     5881 ttcggacata gtggcagaca gctctgcgcg cgccgattcg aagcgggaga ctttcgcgct
     5941 caggtctttc agataacggt cgaattgttt caccgggtcg gtgacaaggt cggagagttc
     6001 ccaatgatcg gcaaactcac gggaggcgga cgaacgacgt gatgacgtag cagcggcaat
     6061 cttgcgcgaa acggccatgg tcgaggctcc tagaaagtaa atgatagaag gaatcatacc
     6121 atcgtcggag gtcaagggcg tggagattct tcgaagacac gcgtctccgg aggcagaggc
     6181 cccgttgaca ggtgcggctc gaatgttaca atccgccgca aggtgaagac atgaccgaac
     6241 aggaaatcaa ccgcgcgatt caatatgtga cagccgccac atcgtacgga cgggacacgg
     6301 tggcggaggt actccggacc ggtttgtcgg aactagcgca attgtccacg cagtcaacct
     6361 gccgttttga gcgggaagac ctcatgggct atctctgtct ctggacgatg aaacgcaccg
     6421 gtcatccgga gccgctggtg cgtgaagtgc tcgatggcgc gggccgctgg ctggatgaag
     6481 tggccacgac ggtggcccag cggcgcgagg cgcaggcgga tggcggggat gacgatgagc
     6541 caggcgcagc aacggcgtaa gtggcgtgtg gatgacgcct gtcgttcgtg acaggtccct
     6601 cggtggatct gtcatctctt acgaaatacg tgtcacgaga gacgcttcac ggactccgcc
     6661 aactccaagc gactgagtga gattggggag tggtcggtca ggcggggagt gcgctcttca
     6721 gcgcttccag atttttcgtc accattgcat cgccgttctt ggtagacacc tcgatgccct
     6781 gatccgacac ctgccggcat tcttcaaccc gattcagctt ctggagcgat tgcgccagcg
     6841 cataataagc ggcggagtag gtgggcttga ccgtgatgca ctgtctcaag gccttggcgg
     6901 cctcttcgaa attggcatct tccatatagg ctttccccag tccgaaccag gcgacatcat
     6961 catcgggttc gagggccagc actcgtttca gcggttcgat tctcggattc ggcattggct
     7021 tctccagggt cgatcggcac ggtgaagcga aaccgtagca cgcgtttccg ctcattgtct
     7081 acgctctttc ctgcatgata gggtgaggga actgtttgtg tcgaaatacg agatacgaac
     7141 gacgcttcac ggacattgac atgggaacaa ccgggctcat ttacgatcca cgttacctgg
     7201 aacacgacat gggcgccgga catccggagt cgcccaaccg gctgcgcgcg attatgcagc
     7261 ggctcgaaca gagcggtacg ctggccacac tcaccaggat tgacccgcgc acagccgaag
     7321 acgagtgggt cacgctggtg catcggtcgg agtatgtcgc gatgctcaat cgacaggcgc
     7381 cgacgcatgg ccggatctcg ctcgacgccg atacctccat gtcacccgga tcgctcggcg
     7441 ccgcctatct cgcggccggt ggagccttgg cgggagtgga tgcgattatg gccggccggg
     7501 tccagcatgc tttctgtgcg gtccgtccgc cggggcacca tgcggaagcc gatcgggcga
     7561 tgggtttctg cctgttcaac aatgtggcga tcgcggcgcg gtatgcgcag aaacggtatg
     7621 gcgtgcaacg ggtcctgatc gtcgattggg acgttcacca cgggaacggg acgcagcaca
     7681 gcttcgagtc agatgcgtcg gtgctgtttt tcagtacgca ccagtatccg cactatcccg
     7741 ggaccgggcg cgccagcgag tgcggccgcg gagccgggga agggctcacg atcaatgtgc
     7801 cgatggaggc gggggaaggc gacgacgaat accgggcggt gtttcagaag gttctggtgc
     7861 cggctgccga tgccttcaag ccggagtttg tcatcatctc cgccggtttt gacgcccacc
     7921 gggacgatcc gctcgcgagc atggggttga ctgaagaagg gtatgccgat ctgacgaata
     7981 ttgtagccgg aattgccagg caacattgcc aaggacgcct gctgtcggca ctcgaaggcg
     8041 ggtacaacct cacggcgttg gccgcgtcgg tcgagcgaca tattc
//
LOCUS       DAOQRM010000111         8041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_8543_length_8041_cov_15.924493, whole genome shotgun sequence.
ACCESSION   DAOQRM010000111 DAOQRM010000000
VERSION     DAOQRM010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8543_length_8041_cov_15.924493"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1346
                     /locus_tag="PKK81_09325"
     CDS             72..1346
                     /locus_tag="PKK81_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33120.1"
                     /translation="MNLWVKILLGVAVLAGAIYLYYTEVKPVVIFGLRSDYAHAIPFQ
                     KVPEGLTSLKAESCGQCHREIYEEWKTSIHAHAYEDPFFQAYWKKDKNIWVCLNCHTP
                     LENQQPTLVKDIPRGRVEKATQEPNPHFDADLRKESITCAACHVRDGVILGPFDDSAA
                     PHPTKFDPSFRNAQFCSRCHNVVSGPAQFYNVGPCGTYAEYEGKYFMQERGFICQSCH
                     MPEVDRPVAENGPIRRGRRHLWRGGHDPDMVKRAVAIQVKADPPAPKPGEQVTVTLSL
                     MNAGAGHKIPTGDPDRHFTVEFTVQDSNRQVLAEKSHTMGRWIMWQPAIVELYDNRLL
                     PLASRDYQFAYRMPEASKGLTLKTRVRYHILTDGQHEMLKTKYGLMADDPYAFTVYER
                     EVPLNETLAAALVDPLPEPPPMACASPTVSKG"
     gene            1375..2268
                     /locus_tag="PKK81_09330"
     CDS             1375..2268
                     /locus_tag="PKK81_09330"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNV33121.1"
                     /translation="MQHPLLIDCETLQKRLGQPGLVVLDVRGRAAYEFGGHIPGAVHS
                     TWHEYSDPDAIAKGLLDPDLKRIEKRVQALGINQESEVVIYSNPFDNWGDEGRMFWML
                     EYLGHKNLRVLDGGWVKWIHERRPFEHGAVRCAPGNFVVSPVESAIIMKNELKGIARK
                     PNPTTTIVDARSLEEYLGKEISGIPRPGHIPGAVHVAWSGFLNADATVKDLAAINAQL
                     DLKGLNPTHETICYCTGGVRSAWLYFILRLAGYQRLRNYPGSWWEWSRDFACPVEKDL
                     LALQKLLGLSESQVPTGMRPS"
     gene            2349..2705
                     /gene="smbP"
                     /locus_tag="PKK81_09335"
     CDS             2349..2705
                     /gene="smbP"
                     /locus_tag="PKK81_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HNV33122.1"
                     /translation="MQTAKWRGVVVAGVMMALVGAPMVAGVAFAAGNKHQAEAVEHAK
                     EAVAHGKQGHADALVKHAEAALKHAEGAVAETKNPHVTEAIKGLKDGIEHGKAGHADV
                     ATKAVENALPHLSEGM"
     gene            2790..3581
                     /locus_tag="PKK81_09340"
     CDS             2790..3581
                     /locus_tag="PKK81_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNV33123.1"
                     /translation="MRVGYLQFDPVFGEVAHNLDLITARLEEAEADLLVLPELCASGY
                     QFVSQEEVFRLAEPVPDGATTKRLVEIAARRGMTIVAGLPERDGRHCFNSAVVVGPKG
                     FIGCYRKTHLFFEETLWFTPGDSGFHVWDIGMAKVGVMICFDWYYPESARTLALQGAD
                     IIAHPSNLVLPHCPDSMVTRCLENRVFSVTANRIGSEARGGKDRLTFIGMSEVVSPRG
                     RILHRAPRETEDLTIVEIDPAEARIKALNDYNDLLRDRRPALYGD"
     gene            3646..4206
                     /locus_tag="PKK81_09345"
     CDS             3646..4206
                     /locus_tag="PKK81_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNV33124.1"
                     /translation="MSIPLGKGILLVAAPALNDPNFRQTVVLLCEHGPEGALGVIVNR
                     PTAMSISEALPQVPILEGQPHVLYSGGPVQTNQVMMLYRINQTPENSHQVFDGVCLGG
                     DLEIMERILMEQPGQESFRAYLGYSGWGPGQLESEMQVGSWITLPADPSIVFEKEPTR
                     IWSDIFLSLDDTSRHYADMPFDPSSN"
     gene            complement(4522..7323)
                     /gene="uvrA"
                     /locus_tag="PKK81_09350"
     CDS             complement(4522..7323)
                     /gene="uvrA"
                     /locus_tag="PKK81_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNV33125.1"
                     /translation="MAHRSPPDRTTNDLIVEGARQNNLKNISLRIPHNRVTAITGLSG
                     SGKSSLAFDTLFAEGQWRYVESLSTYARMFLDKVNRPDVDRITNIRPAIAIEQKNPIR
                     TARSTVGTATELADLLRLLFAKIGKPVCPDCKQEARGYHPGSVAEELLARFPDARAMV
                     LFPLKDLGPGHDRSLLDSLLKRGFTRLRCGEELLDLHEQAVLPVTRESGIQVVLDRLV
                     LRPDNRHRLIEAIEVAFQEAEGTCQVLVIGQGLRTYSTHFRCQGCGRTFEPLRPLLFS
                     FNHPLGACPECKGFGNILQYDKDLVIPNRSKSLAGGVIEPWSKPGSDWWQKQILLAMK
                     KQGVDLTAPFQELPEEVQQLIWEGSDHVEGVRQYFDYLETKRYKLHVRVLLSRYRSPA
                     TCPTCHGSRLKPAARLVKLAGQDIVEIGELTIEAAAAWFERLALPAFDAEVAKDILRQ
                     LHAKLNFLLRVGLSYLTLSRQTKTLSGGEAQRIALANQLGSRLVGTLYVLDEPTIGLH
                     ARDTDTLAGILRDLANHGNTVVVVEHDPSMIQAADHIVEMGPGSGEQGGHIVCAAPRE
                     QFLADQASLTARYLRGENRIPLPKIRRSGNGKVLSIAGAAEHNLKNLVVRIPLHMLVC
                     VTGVSGSGKSTLIEKTLYRAAARAFRIESLPMGKFQAIKGLEHVKGVRLIDQQPIGRT
                     PRSNPITYLKAFDEVRTLFASEREALRRGLTPGHFSFNSADGRCERCEGNGYEKLEMY
                     FFEDIYATCEQCDGKRFKPEVLSIRYRGKTIHDVLNLTVTDAQAFFSGSPKLTEKLYL
                     LSSIGLGYLRLGQAATTLSGGEAQRLKIAAELKDPSAHNLLYILDEPTTGLHLDDIKK
                     LLTVLHKLVDAGNTLIVVEHNLDVIKTADWVIDLGPEGGEAGGQIVAEGRPEQVAKVT
                     QSHTGKFLAKVLGGAPRTS"
     gene            7467..>8041
                     /locus_tag="PKK81_09355"
     CDS             7467..>8041
                     /locus_tag="PKK81_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HNV33126.1"
                     /translation="MVDRSAYLARIGYQGSVVPSMETLRGLHLAHVLTVPFENLDIHL
                     GRPISLDPSDLFRKIVISRRGGYCFELNGLFALLLEELGFVVTRLAARVLYGTEGVRP
                     RSHQVLLVHLDGERWIVDVGFGGQGLREPLPFVVGLEHRQGPDQFRLATDERGEHVLQ
                     CRIEEAWTNLYSFALDPWLPVDYQFANYYHS"
BASE COUNT         1522 a         2261 c         2564 g         1694 t
ORIGIN      
        1 ctcgaaggcg ggtacaacct cacggcgttg gccgcgtcgg tcgagcgaca tattcaggcg
       61 ctggtggcgg catgaacctg tgggtgaaaa tcctgctggg tgtcgcagtc ctcgccgggg
      121 cgatttacct ctattacacc gaggtgaaac cggtcgtcat tttcggactc cggtcggatt
      181 atgcccatgc cattccgttt caaaaggtgc cggagggcct gaccagcctg aaggccgagt
      241 cctgcggcca atgccaccgg gaaatttatg aggagtggaa aaccagcatc cacgctcatg
      301 cctatgaaga tccgttcttt caagcctact ggaaaaaaga caaaaacatc tgggtctgtt
      361 tgaattgcca cacgccgttg gagaaccaac agccgacgct ggtgaaagac attccccggg
      421 ggcgggtcga gaaagcgacg caggaaccga atcctcattt tgatgccgac ctgcggaagg
      481 aatcgatcac ctgtgccgcc tgccatgtgc gcgacggagt gatcctcgga cccttcgacg
      541 attccgctgc gccgcatccc acaaaattcg accccagttt ccgcaacgcg cagttctgtt
      601 cgcgttgcca taacgtggtc tccggtccgg cgcagtttta caacgtcggc ccctgcggga
      661 cctacgccga atatgaaggt aagtatttca tgcaggagcg gggcttcatt tgccaaagtt
      721 gccatatgcc ggaggtcgat cggccggtgg ccgaaaacgg gccgatcagg cgcgggcggc
      781 ggcatctctg gcgcgggggg cacgatcccg acatggtgaa gcgggccgtg gcgattcagg
      841 tgaaggccga tcctccggct cccaaaccgg gtgagcaggt cactgtgacc ctctccctga
      901 tgaatgccgg ggcggggcac aaaattccga ccggcgatcc ggaccggcac tttacggtag
      961 aattcacggt tcaagactca aaccggcagg tgctggccga gaaatcgcac acgatgggcc
     1021 gctggattat gtggcaaccg gccatcgtag agttgtacga taaccgcttg ctccccttgg
     1081 ccagccggga ctatcaattt gcctaccgga tgccggaggc gtccaagggg ttgacgttga
     1141 agacccgggt tcggtaccac atcctgaccg acgggcagca tgagatgttg aagaccaagt
     1201 acggcctgat ggcggacgat ccctacgcct tcacggtcta tgagcgggaa gtgccgctga
     1261 atgaaacgct ggcggccgcg ttggtcgatc ccttgccgga gccgccgccg atggcctgtg
     1321 cgagtccgac cgtatccaaa gggtaagtca tcacccaccg agatcgagga acccatgcaa
     1381 catccattac tcatcgattg tgaaacgctt cagaaacgct tggggcaacc gggccttgtc
     1441 gtgctcgatg tgcgagggag agccgcctat gagttcggag gccatatccc cggtgccgtg
     1501 cattccacct ggcatgaata cagcgatccc gatgccatcg ccaaggggtt gctggatccc
     1561 gacctgaaac gcatcgagaa acgtgtgcag gcgctcggca tcaatcagga gagcgaagtg
     1621 gtgatctatt cgaacccctt cgataattgg ggcgacgaag gacgcatgtt ctggatgctg
     1681 gaatacttag gccacaaaaa cctcagagtg ctggacggcg ggtgggtgaa atggatccat
     1741 gaacgccggc cgttcgagca tggcgcggtc cgctgcgcgc ccggcaactt cgtcgtgtcc
     1801 cctgtagagt cggcgatcat catgaagaac gagttgaagg ggattgcgcg gaagccgaac
     1861 ccgacgacca cgatcgtgga tgcgcggagc ctggaagagt atctggggaa agaaatctcc
     1921 ggcattccga ggcccggcca tattcccggt gccgtccatg tggcgtggag cggatttttg
     1981 aatgccgatg cgacggtcaa ggatctggcc gccatcaacg cgcagttgga tctcaagggg
     2041 ctgaacccga cacacgaaac gatctgctac tgcaccggcg gcgtgcgctc ggcgtggctc
     2101 tatttcattt tgcgactggc cggttatcag cggttgcgta attatccggg ctcctggtgg
     2161 gagtggagcc gcgatttcgc ctgcccggtg gaaaaggatt tgctcgcgtt gcagaaactt
     2221 ctcggcctgt cggagtcgca ggttccgacc ggcatgcgtc cgtcttgaca ggatgcaggc
     2281 ccctctttat gctgaaatac agtatcgttg gaatcatgcg gtgcacatac ttacagagga
     2341 ggctgttcat gcagactgcg aaatggcggg gagtcgtggt agccggtgtg atgatggcgc
     2401 tcgtcggggc gccgatggtt gccggtgtgg ctttcgcggc aggaaataag catcaggccg
     2461 aagcggtgga gcatgccaaa gaagccgtgg cgcatgggaa gcagggccat gcggacgcct
     2521 tggtgaagca tgccgaagcg gcgctcaagc atgccgaggg tgcggtggcc gaaaccaaga
     2581 atccgcatgt cactgaagcc atcaaggggc tcaaggacgg gatcgagcac ggcaaggccg
     2641 gccacgccga tgtggcgacc aaagccgttg agaacgccct tccccatctg tcggaaggta
     2701 tgtaatcggt tcttgcgcgt cgacggcgtg taagatgaaa cgggcagggc cgtccctgcc
     2761 cgtttttctt tgagtagaaa ggcgacatca tgcgggtcgg gtatctccaa tttgatccgg
     2821 tcttcggcga ggtcgcgcac aatctcgacc tcatcacggc tcgacttgaa gaggcggagg
     2881 ccgaccttct tgtgctgcct gaattgtgcg cgtcgggtta tcagtttgtg tcgcaggaag
     2941 aggtgttccg gctggcggag ccggtgccgg acggggcgac cacgaaacgg cttgtggaaa
     3001 ttgccgcacg ccgtgggatg accatcgtag ccgggttgcc ggagcgggac ggacggcact
     3061 gctttaattc ggcggtcgtg gtcggaccca agggattcat cggctgctat cggaaaacgc
     3121 atctcttttt cgaggagacg ctctggttta ctccgggcga cagcgggttt cacgtgtggg
     3181 atatcggcat ggccaaagtc ggcgtgatga tctgcttcga ctggtattat ccggaatccg
     3241 cccgcacgct ggcgttgcag ggcgcagata tcatcgcgca tccgtcgaac ctggtgctgc
     3301 cacattgtcc tgattccatg gtgacccgct gtttggaaaa tcgagtcttc agcgtgacgg
     3361 cgaatcggat cggtagcgaa gcgcgcggag gaaaggaccg gctgacattt atcggcatga
     3421 gcgaagtcgt cagtccgcgc ggccggatcc tgcatcgcgc accgcgtgaa accgaggacc
     3481 tgacgatcgt cgagatcgac cccgccgagg ccaggatcaa agcactcaat gactacaacg
     3541 atctcctgcg cgaccggcgc ccagccttgt atggcgacta atcccgcgtc gtatacgttt
     3601 catgactcaa cttgcgtcac ttacgagcac caggtaggat gaaccatgtc aattccactc
     3661 ggtaaaggca ttctcctcgt ggccgcgccg gcgttgaacg atcccaactt ccggcagacg
     3721 gtggtgctcc tgtgtgagca tgggccggaa ggggcgctgg gtgtgatcgt caaccgtccg
     3781 accgccatgt ccatctctga ggcgcttccg caggtgccga tcctggaagg tcaaccgcat
     3841 gtgttgtatt ccggcggccc cgttcaaacc aatcaggtga tgatgctcta tcgcatcaat
     3901 cagacgcccg aaaattcgca ccaggtgttt gacggtgtct gtctgggcgg cgacttagag
     3961 attatggaac ggatcctcat ggagcagccc gggcaggagt cgtttcgagc gtacctgggg
     4021 tattcgggct gggggcccgg ccagctggag tcggaaatgc aggttggttc ctggatcacg
     4081 ttgcctgccg atccctcgat tgtgttcgaa aaagaaccga cccgcatttg gtcggatatc
     4141 ttcctctcac tggacgacac ctcccgccat tatgcggata tgccgttcga tccctcctcc
     4201 aactaggcag cccccattat catggcgggt tctactgcaa ccgcagctac gccgctgcga
     4261 caagcctggt cgccaattgt tcgcgaccag gcgggcgggc tcgccgtgcg gtcagtcaac
     4321 atccagttca ggatgcctcc ttcccttacg gctccacgcg cccgtctcgc ttggcgtttc
     4381 tgcgatttcg taacgaaccg ccacgaataa cggggactac ctagttgaag atagtctcaa
     4441 tcgttaagcg tcgttgtgtc tcgtacgacc ttgacgatcg agctattgca ccatggcatc
     4501 ggacgagata cgcttcacgt ttcacgaggt gcggggggct ccgcccaaca ccttcgcgag
     4561 aaatttcccc gtatgagact gtgtgacttt cgcgacctgt tcggggcgtc cttcggccac
     4621 gatctgtccg cctgcttcgc ctccttccgg tccgaggtcg atgacccaat cggcggtctt
     4681 gatgacatcc agattgtgtt cgacgacgat gagggtattc ccggcgtcta ctagcttgtg
     4741 cagaacggtc agcagcttct tgatgtcgtc caggtgcagg ccggtggtcg gctcatcgag
     4801 gatgtagagc aggttgtggg cggacggatc tttcagttcg gcggcgatct tcagccgttg
     4861 ggcttctccg cctgacagcg tggtggccgc ttggcccagc cgcagatagc ccaatccaat
     4921 cgaggacagc aggtagagtt tttcggtcag tttcggcgat cccgagaaga aggcctgcgc
     4981 atctgtcacg gtgaggttca gcacatcgtg gatcgtttta ccccggtacc ggatgctcag
     5041 cacctctggc ttgaagcgct tgccatcgca ctgttcgcag gtggcgtaga tgtcttcgaa
     5101 gaaatacatc tccagctttt cgtacccgtt tccttcgcag cgctcgcagc gtccatcggc
     5161 ggaattgaag gagaaatgcc cgggagtcag gcctcggcgc agcgcctccc gctcggaggc
     5221 gaacagagtg cggacctcgt cgaatgcctt caggtaggtg atggggttgg agcgcggcgt
     5281 ccggccgatc ggttgttggt cgatgagccg cacgcctttc acatgttcga gtcccttgat
     5341 ggcctgaaac tttcccatcg ggagcgactc gatgcgaaag gcgcgggcgg cggcacggta
     5401 cagcgtcttt tcgatgaggg tgcttttgcc cgatcccgag acgccggtga cgcagaccag
     5461 catgtggagc ggaatgcgga ccacgagatt cttgaggttg tgctcggccg cgccggcgat
     5521 gctcaggacc ttgccgttgc ccgagcgtcg gatcttcggc aggggaatgc ggttctcccc
     5581 gcggaggtaa cgggcggtga gtgaggcctg atcggcgagg aattgttcgc gtggcgccgc
     5641 gcagacgatg tgcccaccct gctcgcctga accgggtccc atttcgacga tgtggtcggc
     5701 ggcctgaatc atcgacggat cgtgttccac cacgacgacg gtgttgccat gattggcgag
     5761 atcgcgaagg atcccggcca gggtatccgt gtcacgggca tgcaggccga tcgtcggctc
     5821 atccaatacg tagagtgtgc ccacaaggcg agagccgagc tggttggcca acgcgatccg
     5881 ttgcgcttct ccgccggaga gggtttttgt ctgccgcgac agggtgaggt agctcaatcc
     5941 tacgcgcagc agaaagttca gcttcgcgtg cagttggcgc agaatatcct tggcgacctc
     6001 cgcgtcgaag gcgggcaggg ctaagcgctc aaaccaggcg gctgccgctt caatggtgag
     6061 ctcaccgatt tccacgatgt cctgtcctgc cagtttgact aaccgggcgg ccggcttcag
     6121 gcggctgccg tgacaggtcg ggcaggtggc gggactgcgg tagcggctga gcagcacccg
     6181 cacatgcagc ttgtagcgtt tggtttccag atagtcgaag tattgccgga ctccttcaac
     6241 gtgatcgctc ccttcccaga tcagctgttg cacctcttcg ggaagctctt gaaacggggc
     6301 cgtcaaatcc acgccctgtt tcttcatcgc cagcaatatt tgtttttgcc accagtccga
     6361 gcccggcttg ctccagggct cgatgacgcc gccggcgagc gatttgctgc ggttggggat
     6421 gaccagatcc ttgtcgtatt gcaggatatt gccgaaccct ttgcactccg ggcaggctcc
     6481 gagtgggtgg ttgaaggaga acagcagcgg gcgtagtggt tcgaaggtcc gcccgcaccc
     6541 ctggcagcga aagtgggtgc tgtaggtcct gagcccctgc ccgatgacca atacctgaca
     6601 ggtgccttct gcttcctgga acgccacttc gatggcctcg atcaaccggt gccggttgtc
     6661 cggtctgagg acgagtcgat ccagcacgac ctggatgccc gattcgcggg tcaccgggag
     6721 cacggcctgc tcgtgaagat ccaggagttc ctcaccgcat cgcagccggg tgaatccccg
     6781 tttcaaaagt gagtcgagca gcgagcggtc gtggccaggg ccgagatcct tgaggggaaa
     6841 aagaaccatg gcgcgcgcgt cggggaatcg tgccagcagt tcctcggcga ccgaccccgg
     6901 atggtaacca cgcgcctcct gtttgcaatc cgggcagacg ggcttgccga ttttggcgaa
     6961 gagcagccgc agcaggtcgg ccagctcggt ggcggtgccg acggtcgaac gggcggtgcg
     7021 gatcggattt ttctgttcga ttgcgatcgc ggggcggatg ttggtgatcc gatcgacgtc
     7081 gggccggttc accttgtcga gaaacatccg tgcgtaggtc gagagcgatt ccacatagcg
     7141 ccattgtcct tccgcgaaca gtgtgtcgaa ggccagggac gatttgccgg atccggacag
     7201 cccggtgatg gcggtgaccc ggttgtgagg gatccgcagg gagatgttct tgagattgtt
     7261 ctggcgcgcg ccttcgacga tcaagtcgtt cgtggtgcga tctggcggac tacggtgtgc
     7321 cacggcagcg gttccttccg gagaaagacc gaccatcgta gcaagtgccg cagtcgtctg
     7381 caatgtcacc tgtcggcgtc ggcgctgaga tccgtgtgat ccgcccgggc agattcgtat
     7441 aatggctctc tcatcgggag gactctatgg ttgatcgatc ggcctatctc gcgcgcatcg
     7501 gatatcaagg ctcggtggtt ccgtcgatgg agacgttgcg cggtttgcat ctggcccatg
     7561 tcctgaccgt gccgttcgaa aatctggaca tccacctcgg tcgcccgatt tcgttggatc
     7621 cctccgatct gtttcggaag atcgtgatca gtcggcgggg cgggtattgc ttcgagttga
     7681 acggattgtt tgcgctcctg ttggaggagc ttggttttgt cgttacgcga ctagctgccc
     7741 gggtgttgta tgggacggaa ggcgtcaggc cgcggagtca ccaggtgcta ctggttcatc
     7801 tggacggaga gcgctggatt gtggatgtgg gattcggagg gcagggattg cgtgagccgt
     7861 tgccctttgt ggttggtctg gaacatcggc aggggccgga tcaatttagg ctcgcgaccg
     7921 acgagcgggg agagcatgtg ctgcaatgtc gcatcgagga ggcctggacg aatctctatt
     7981 ccttcgcact cgacccctgg ctgccggtgg actaccagtt tgccaactat taccactcgc
     8041 a
//
LOCUS       DAOQRM010000112         8027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_8570_length_8027_cov_14.862519, whole genome shotgun sequence.
ACCESSION   DAOQRM010000112 DAOQRM010000000
VERSION     DAOQRM010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8570_length_8027_cov_14.862519"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKK81_09360"
     CDS             complement(<1..566)
                     /locus_tag="PKK81_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV33127.1"
                     /translation="MDVLSEVLKAVKLDGAVFFHGEFAAPWCAREPDSGTMASYLPTQ
                     AGHIIIFHLLLEGRGYVRLEQEDRAVPLTAGDIIVLTHGDAHFMGNGPPVTPVDTSQQ
                     IRQILSEGRMLSQLAGGGEVTKLICGYLTCDAQLCRVVLAGLPAMLKVNIRDTPSGQW
                     LENTLRYSVDHAEASGPGGAAVIAKLSEA"
     gene            764..1585
                     /locus_tag="PKK81_09365"
     CDS             764..1585
                     /locus_tag="PKK81_09365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV33128.1"
                     /translation="MTTNTVVPEMDGLKTRLNTIWTAGDYDRFSRYMEGGAREFYERL
                     QIAPGCRLLDVGCGSGQLALLAAKDGVEVTGVDIAANWVERAQARARAEGVTARFEVA
                     DAEALPFETASFDVVVSLIGAMFAPRPDLVAQELLRVCTPGGTLAMANWTAEGFVGQM
                     FKNVAKFIGPSGMPSPVLWGNEATVQERLGHGLSTLSLIRRQYLFSYPFPPSEVVEFF
                     RLYYGPTNRAFASLDAEGQAHLRRDLEALWTAHNQAGPDCTTVYGEYLEVIGIRA"
     gene            1617..1961
                     /locus_tag="PKK81_09370"
     CDS             1617..1961
                     /locus_tag="PKK81_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNV33129.1"
                     /translation="MTNATTTEMDQTTGLYDMTNLEKMKDLGTHAPEAMKAFVAFDKA
                     ALAAGAIPVKYKELMAMAVAFTTQCPYCIELHTNKAREYGASDPEIAESVLVAAALRA
                     GGAITHGTHAMK"
     gene            2064..3239
                     /locus_tag="PKK81_09375"
     CDS             2064..3239
                     /locus_tag="PKK81_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNV33130.1"
                     /translation="MKRYRLFAFTLGLLLSCSTLASAEILALLNYESKPDQPVRREGI
                     AIMDIDPESGNFGKILMEIPLPPDLVAHHIFFNRDRSKAYITALGKSILHVVNLRTFP
                     YRLQAIDVPDCQMGEDLAVSEDNRTWYLTCMGSDNVIMGDALLDKPIKAVSAAKPSLA
                     TIQYPHGIAIHNGIDRLLVTSTVKPDMSDAGDSITVLEAASGKVLSTHKVSSKPSPAK
                     AAPVEVMFSPNANPPVVHITNMLEGTLWAGVWDPASRTFSFHQVDDFGSRQQGMPLEM
                     LYNAKGDRLFVTTAKPGFVNLYDNSDPRQPKFLKTIAAAGGAHHSVLSPDERYLFVQN
                     SFLNLDGMNDGSITVIDLKADKVLGNIDTLKAQGFNPNCIMLLPNQPGDLRASRIID"
     gene            complement(3331..4365)
                     /locus_tag="PKK81_09380"
     CDS             complement(3331..4365)
                     /locus_tag="PKK81_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNV33131.1"
                     /translation="MPENNHSNAPLPPPHVQLIQMAMAHWVSRIAYVAAKLGLADHLA
                     SGPKTADDLAGPTGTHAPSLYRLMRALTNLNILTEDATHRFALTPLGEALKTGAPGSA
                     RPSILTLASDWMSRGWEQLLYSVQTGGCGFEQSLGMPLFDWLAKHPEDASLFSETMMG
                     FHGAEPAAITAAYDFSGLSTLIDVGGATGHLLTTILTGNPASRGILYDLPHVVRDAPA
                     LIQARGLADRVTIEAGSFFDRVPGGGDAYLLSHIIHDWTESQCLTILGHCRRAMTPGS
                     RLLIIEMVLPSDNAPHPGKMLDMMMLVGPGGQERTESEYRELLGKAGFRLTRLVPTDT
                     AVSVVEAVLT"
     gene            complement(4753..5976)
                     /locus_tag="PKK81_09385"
     CDS             complement(4753..5976)
                     /locus_tag="PKK81_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV33132.1"
                     /translation="MTPPSLPLPHDHPERPSTHGAPAPHPDAPRPSEAELDRFFELSL
                     DMLCIANAESYFTRLSPAFTRTLGWSLEELMSRPFLDFVHPDDRANTLQEVERQIGRG
                     EPVLEFQNRYQHKDGSWRLLSWKSSPHPDGRLYAIARDVTDRELAAQALRRSHEELER
                     RVQERTAELQQRNRDLETLLYVSSHDLREPLRSISNFSQLVRERYGTCLDAKGLDYID
                     RVIRAAQRMDQLMEDLLTLSRAQRMEMPYEEVRGEDLVKAAWAQLDETIRRTRARIQV
                     ATDFPSFRVNKTWVTQALYNLIGNALKFHRAGEAPEIDLAPYSLMEGSDHRVGLVVRD
                     RGIGVEPEQAQRIFHLFYRAVGREVHGTGAGLAIVQQVAHRHGGRAWVQPREGGGSEF
                     VLLFGSHSTIDTKTT"
     gene            complement(6825..6947)
                     /locus_tag="PKK81_09390"
     CDS             complement(6825..6947)
                     /locus_tag="PKK81_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33133.1"
                     /translation="MVQALSTISDVERVRQYQKLIELELLKMNTFVAAEMTPNL"
     gene            complement(7060..7578)
                     /locus_tag="PKK81_09395"
     CDS             complement(7060..7578)
                     /locus_tag="PKK81_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33134.1"
                     /translation="MDIISHGLWGAFAFGRRNRTSFSLAFAIGMAPDLFSFGILWVAA
                     TLGLSPTPDFSHGTPPESTIPPYVHYLYNVTHSFVLFLAVFALLWLWTKRPVWELGAW
                     GLHVLVDIPTHSFAFFPTPFLWPLSDWKFDGWQWNQSAILIPNFTVLFLLYAWFLLQQ
                     GRPALRPERVRE"
     gene            7771..>8027
                     /locus_tag="PKK81_09400"
     CDS             7771..>8027
                     /locus_tag="PKK81_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33135.1"
                     /translation="MAFNQVGDFWLAPGQSTRVHIALGGLVNEAEWGGHDFGAQWIMA
                     DGVGINPVRLMVSQHTKEKKPIRLHPGSPSPIVYSVTVTNIG"
BASE COUNT         1620 a         2292 c         2558 g         1557 t
ORIGIN      
        1 gcttcggata acttggcgat gaccgccgcg cctccgggac cggaggcctc cgcgtgatcg
       61 actgaatagc ggagcgtatt ttccagccac tgtcccgacg gcgtatctcg aatattcacc
      121 ttgagcatgg caggcagccc ggccaggacg actcggcaaa gctgcgcgtc acaagtcaaa
      181 tacccgcaga tcagtttcgt gacttcaccg cctccggcga gttgggacag catgcgtccc
      241 tccgagagga tctgccggat ttgctgggag gtgtccaccg gtgtgacggg cgggccgtta
      301 cccataaagt gcgcatcccc gtgcgtgaga acgatgatgt cgcccgccgt cagcggcacg
      361 gcacggtctt cctgctccag gcggacatac ccccgtcctt cgagcagcag atggaaaata
      421 atgatgtggc cggcttgggt aggcagatac gacgccatgg tgccggagtc cggctctcga
      481 gcgcaccagg gcgcggcaaa ctcaccgtga aagaacaccg cgccgtcgag tttcacggcc
      541 ttgagcactt cggacagcac gtccatagga gtttcctcgg caaggccgga gcctgagcaa
      601 agccatccgg acgcccggat aaacgaactg cagcttagca tgctcctgat aagagagcca
      661 gacgccctga taagactcga aacggcctct gtcctatagt gcgaccagac aagacgcagc
      721 gacggaagcc aggacagaga acacacacgg gaaggaggag gccatgacga cgaacacggt
      781 ggtgccggaa atggacggtc tgaagacccg tctcaatacc atttggacgg caggggacta
      841 cgatcgattt tcacgatata tggagggcgg cgccagggag ttttacgaac gtctccagat
      901 cgcgccggga tgccggttgc tggatgtggg atgcggctcg ggccaactcg cgttgttggc
      961 cgccaaggat ggggtggagg tgacgggggt ggacatcgcc gccaattggg tcgagcgggc
     1021 gcaagcgcgg gctcgggccg aaggggtgac tgcccgattc gaagtcgccg atgccgaagc
     1081 gctgccgttt gaaacggcca gtttcgacgt cgtggtgagt ctgatcggcg cgatgtttgc
     1141 tccgcgtcct gatctggtgg cgcaggaatt gttgcgggtg tgtacaccag ggggaaccct
     1201 cgcgatggcc aactggacgg cggaggggtt cgtcggacag atgttcaaga acgtcgcgaa
     1261 attcatcggg ccgtcgggta tgccgtcgcc ggtgctctgg gggaatgaag ctacggttca
     1321 agaacgcctt ggtcacggat tgtccacgct cagcctgatc aggcgtcagt atctgttcag
     1381 ctatccattt cctccatcag aggtggtgga attcttccgt ctctactatg gaccgaccaa
     1441 ccgggccttt gcgtccctcg acgccgaggg gcaagcgcac ttgcgccggg atctggaagc
     1501 gctctggaca gcgcacaatc aagcagggcc ggattgcacg acggtgtacg gcgaatattt
     1561 agaagttatc ggcattcggg cgtgagcctc gattgcccat acaaaggaga tgcatcatga
     1621 cgaacgcgac cacaaccgag atggatcaga cgacgggcct gtatgacatg acgaatctgg
     1681 aaaagatgaa ggatctgggg acgcacgccc ccgaggccat gaaggcattc gtggccttcg
     1741 acaaggccgc gctggcagcc ggcgcgattc cggtgaagta caaggaattg atggccatgg
     1801 ccgtggcgtt caccacgcaa tgtccctact gcatcgaact gcatacgaac aaggcaagag
     1861 agtatggcgc gtcggacccg gagatcgccg aatcggtgct cgtggcagcg gccttgcgcg
     1921 caggcggggc catcacgcat ggcacccatg cgatgaagtg aatgtgaggg gggcggcggg
     1981 ccgagagagc cggccgtgca tacgcctgga caacgtactc agccaaagtg agtccaattt
     2041 cgacaatcga aaaggaggcc accatgaaac gatatagatt gttcgcgttc accctggggt
     2101 tgttgctttc gtgctccacc ctcgcatcgg cggaaatcct ggctctgctc aactatgaga
     2161 gtaagccgga ccagccggtg agacgggagg ggattgccat catggacatc gatcccgagt
     2221 ccgggaactt cgggaagatt ctgatggaga ttccactgcc gcccgatctc gtggcgcacc
     2281 atattttctt caatcgcgat cggagcaaag cctatatcac ggcgctgggg aaaagcattc
     2341 tgcatgtcgt gaatctcagg acgtttccct atcggcttca ggcgatcgac gtgccggatt
     2401 gtcagatggg ggaggatctc gccgtgtcgg aggataaccg gacctggtac ctgacctgca
     2461 tgggctcgga caacgtcatc atgggggacg cgctcctcga taagccgatc aaagcggtca
     2521 gcgccgcgaa gccgtccctg gcgacgattc agtatccgca cggcattgcg attcacaacg
     2581 gcatcgatcg cctgctcgtg accagtaccg tcaagccgga tatgtccgat gcgggtgatt
     2641 cgatcacggt gctggaggcc gccagcggca aggtgctctc aacgcacaag gtctcgtcga
     2701 aaccatctcc ggcaaaagcc gctcccgtgg aagtgatgtt cagtcccaat gcgaatcctc
     2761 ctgtcgtgca tatcaccaat atgctcgaag gaacgttgtg ggccggggtg tgggacccgg
     2821 caagcagaac cttttcgttc catcaggtcg atgactttgg ctcccggcag cagggcatgc
     2881 cgctcgaaat gctttacaac gccaaggggg accgcctgtt cgtcacgacg gccaaaccgg
     2941 gtttcgtcaa tctgtacgac aacagcgatc cccggcaacc gaagtttctc aagacgatcg
     3001 ccgccgcggg cggtgcgcac cacagcgtcc tgtcgccgga cgagcgctac ctgttcgtcc
     3061 agaacagctt cctcaatctg gacggcatga acgatgggtc catcacagtg atcgacttga
     3121 aggcggataa ggttttgggg aacatcgaca cgctgaaggc gcaggggttc aacccgaact
     3181 gcatcatgct gctgcccaat cagccgggcg acctgcgggc cagtcgcatc atcgactgag
     3241 ccggcgggga cgtcctgagt cgagaaagcg ccaggagtac ggcgggagcc ctgtctgtcg
     3301 ccgtactctt cgctcacatc agatgtgact ttatgtgagc accgcttcga cgacgctcac
     3361 ggctgtgtcc gtcggcacga gccgggtgag acggaatccc gctttcccga gaagctcacg
     3421 gtactctgat tcagtccgtt cctgtccgcc tggacccacg agcatcatca tatcgagcat
     3481 cttacccgga tgcggagcgt tgtcggacgg cagcaccatc tcgatgatca acaagcggct
     3541 gccgggtgtc atcgcgcggc ggcagtggcc gagaatggtg aggcattgcg actcggtcca
     3601 atcgtggatg atgtgcgaca gcagataggc atcgcccccg cccggcacac gatcaaaaaa
     3661 actaccggcc tcgatcgtca cgcggtcagc caggcccctg gcctgaatca gcgccggtgc
     3721 gtcgcgaacc acatgaggca ggtcgtagag gatgccgcgt gatgcgggat tgccggtgag
     3781 aatcgtcgtc aggagatgtc ccgtggcccc gcccacgtcg atcagggtcg ataagccgga
     3841 aaaatcgtag gcggcggtga tggctgccgg ctccgcgcca tgaaacccca tcatcgtttc
     3901 gctgaacaac gacgcatcct cgggatgctt ggccagccag tcgaacaggg gcatccccag
     3961 cgattgctca aatccacagc ccccggtctg caccgaatag agcagttgct cccagcctcg
     4021 cgacatccag tcgctggcca gtgtcaggat cgaaggccgt gccgatcccg gcgcgccggt
     4081 cttgagcgct tccccgagcg gcgttaaggc aaagcggtgc gtggcgtcct cggtgaggat
     4141 attgaggttc gtgagtgccc gcatcaggcg ataaagcgaa ggggcatggg ttcccgtggg
     4201 cccggcgagg tcgtcggcag tcttcggccc gctcgcgagg tggtcggcca atcccagttt
     4261 ggcggcgacg taggcaatgc gggagaccca gtgggccatg gccatctgta tcaattggac
     4321 gtgcgggggt ggcagcggag cgttgctgtg attgttctca ggcatgccgg gttcctccgg
     4381 gtatgatgtg aatgaagccg atgttccctg tcctcgggaa aacgttatac acggtatggg
     4441 cgcctcatta aatttctttt ctttcaagaa aggacgatgc tccaatttca gaactcgtgc
     4501 cgggctacgt tgccgttcta caaggcagcc ggacagtcgc aagcagatcg aatgggacgg
     4561 gaccatgcgc cgacaagttg ttcaagcagc ggatggaccg ggagatgagg cgggcgagca
     4621 ggggaaacca taggccgagc tgccgagtga gtcgatcggg gaacgagacc gaatgaggtg
     4681 gaagggggag gagtggcccg cggtgcgccg gggaatgtgt gattagaaga cctgaccccg
     4741 atttggtgtg cattacgtag tcttggtgtc gatggtcgag tggctgccga acagcagcac
     4801 aaattcagat ccgccgcctt cccgtggttg cacccaggcc cggcctccat gccgatgcgc
     4861 aacttgttgg acaatggcca gtcccgcgcc ggtgccgtgc acctcgcgcc ccaccgcccg
     4921 gtaaaacaga tggaagatcc gctgtgcctg ttccggttca accccgatcc cccggtcccg
     4981 caccacgagg ccgaccctgt ggtcggagcc ctccatgagg ctgtaagggg ccaggtcgat
     5041 ctcaggggcc tcgccggccc gatgaaactt caacgcattc ccaattaaat tgtagagcgc
     5101 ttgcgtgacc caggttttat tgacgcggaa ggagggaaag tcggtggcca cctggatgcg
     5161 tgctctcgtg cgtcgaatcg tctcatcgag ttgtgcccat gctgccttga ccaagtcctc
     5221 gccacggacc tcttcgtagg gcatctccat gcgttgtgcc ctcgagagtg tgagcagatc
     5281 ctccatgagc tgatccatcc gttgtgccgc ccgaatcacg cgatcgatgt aatcgaggcc
     5341 ctttgcgtcc aggcaggtgc cgtatcgttc gcgtaccagt tgcgagaagt tcgagatcga
     5401 gcgcaggggc tcacgcagat cgtgagagct cacgtacagc aaggtttcca aatcacgatt
     5461 gcgctgctgg agttctgccg tgcgctcctg cacgcgccgc tctaactctt catgggaccg
     5521 tcgtaacgcc tgcgctgcca gctcacggtc ggtgacgtct cgcgcgatgg catagagcct
     5581 gccatccggg tgcggggagg acttccacga cagcaatcgc caggagccgt ctttgtgctg
     5641 atagcggttc tgaaattcca ggaccggttc gcccctgccg atctgacgct cgacctcttg
     5701 gagcgtgttg gcccggtcgt cgggatggac gaagtcgagg aacggtcgac tcatcaattc
     5761 ctcgaggctc cagcccaatg tgcgcgtgaa cgcgggactc aggcgagtga aatagctctc
     5821 cgcgttcgca atgcagagca tgtccaatga cagctcgaaa aatcgatcaa gttctgcctc
     5881 gctcggccga ggcgcatcgg gatggggggc gggtgcaccg tgagtcgacg gccgttctgg
     5941 atggtcatgc gggagaggta gggacggtgg cgtcatgggc ggatcttagt gaaaccgcta
     6001 cagtcgtgca agacgtctct gctcaggcag agaggcaagg ttcccgcatt ggcgctggag
     6061 gggcgttgga gtcctgtttg caggaactcg ccgtgcataa tgtggtgatg gacgtcgaag
     6121 gatcggtcag gtcgcacggc ggacgtgctt cgatgcggta gccaaagcct ccctccaggg
     6181 cctcgccggc agccgctgcc tggcgcgaag tgtcgaaggc acgggatgaa cgaaacagtc
     6241 aggccgcgcg catgcgaaga aataggcgtc gaacgtaaaa ctccttggtg ctcttccggt
     6301 ccggagctac actgtgccca tggacagccg gcaccagcgg ggcgggcgga caggcgtggg
     6361 aatgttcgcc gggtttctga caggcttcct ctggtttttt ctcagtttcg cggccatgtt
     6421 taacggcgtg tttgttctcc tgcttttcct gcctcctgtg tatgcgctat cgggagcgct
     6481 cctcgtgtgg ctcttccgcc tgcctccgat tcatgcggcg ttctccctcg tggggtcggc
     6541 gttaccatgg gtggcctggt tgtggcccgc gaccatctca agctccggtt ggctgaagtc
     6601 cttagtatgg ccggccctcc ttgtgacggc ttttgggatg agtgtactcg gtgcgcacct
     6661 cgtggctgcc agacggcgtc gtaagccgtc ccaggcggac ccgactccgg cctagcgctt
     6721 catcggtggg gcctcgcaat cgtccgctgc gcgttctcgg gatgtctcgt ccgggcgtcc
     6781 ttcagcactc gcatcatgag tccgggcatc gtcacaccgg cagctcacag gttgggcgtc
     6841 atttcagcgg cgacgaacgt gttcatcttg agcagttcca gctcgatcag cttctgatac
     6901 tgccttaccc gctctacatc tgagatcgtc gagagcgctt gcaccacgtc attccagtcc
     6961 ggcgaaatga gtgcgagcga gaggttcggt ctgcggatct gtggtcgatg cgaaggaggg
     7021 aaatggctgg ctgggacgtg ggatcggaat caggtcagtt cactcgcgca cccgttctgg
     7081 cctgagggcg ggacgtccct gctgcaggag gaaccaggca tagagcagga acagcaccgt
     7141 gaagttcggg atgagaatgg ctgactgatt ccactgccac ccgtcgaatt tccaatcaga
     7201 gagaggccag agaaagggcg ttggaaagaa ggcgaaggaa tgggtgggaa tgtcgaccaa
     7261 cacgtgcaag ccccaggcgc cgagttccca gaccggccgc ttcgtccaga gccagagcaa
     7321 ggcaaagacg gcgagaaaca gcacgaaact atgggtgaca ttgtagagat aatgaacgta
     7381 gggcggaatg gtggattccg gcggtgtgcc gtgactgaaa tcaggcgtcg gcgacagacc
     7441 cagtgtggcc gccacccaca ggatgccgaa cgagaacaga tccggcgcca tcccaatcgc
     7501 aaaggcgagg gagaagcttg tgcggttgcg acgtccaaac gcgaacgctc cccagagacc
     7561 atgcgagatg atatccatgc gctgtctttc ctcacgggac tttccgcgac ctcattatag
     7621 tcggaggcct cacgacgagg ctacttcgcg acgttcccaa tgacggaaga tcgctgcaga
     7681 gtcggaacga tcatgtccga cgggaattcg gattcccctg cgcatacccg ttcgctataa
     7741 tgcgcccgaa ctcttggagg gaggtgcttt atggcattta atcaggtcgg agatttctgg
     7801 ctggcgcccg gtcaatccac gcgtgttcat atcgccctcg ggggcctggt gaatgaggcg
     7861 gaatggggcg ggcacgattt cggtgcacaa tggattatgg ccgatggggt cgggatcaat
     7921 ccggtgcggc tcatggtcag ccagcacacc aaagagaaga aacccattcg cttgcatccc
     7981 ggttcgccaa gccccatcgt gtattctgtg accgtgacga acatcgg
//
LOCUS       DAOQRM010000113         8021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_8580_length_8021_cov_21.019207, whole genome shotgun sequence.
ACCESSION   DAOQRM010000113 DAOQRM010000000
VERSION     DAOQRM010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8580_length_8021_cov_21.019207"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKK81_09405"
     CDS             complement(<1..532)
                     /locus_tag="PKK81_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33136.1"
                     /translation="MRQLLVPLQAEAGIFSGTCLASDSGMPVLKKSALDTYLKVRFGP
                     SAELLAYGPIGKETSGARYKQYGYGAPVRLTFRQGGKTRRVVLGTMSPGPFGHEHPAD
                     RAQAMLWDYDCYGRLPGHIDALDVGAFTARGELMSVATAKEFFLLTEWSEGATYHKDL
                     ERMAGATKPTALDRKRT"
     gene            741..1286
                     /locus_tag="PKK81_09410"
     CDS             741..1286
                     /locus_tag="PKK81_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNV33137.1"
                     /translation="MNLEVESRNIAMTPRWKTEIEERMATLQRGHDDIIHGRVTLTKN
                     RHHKKLDNVAEALVLVTVPTRHTITSRKEDKTFEEAIRAAFDAVAIELRKYREKRADK
                     VVRIEPLPQLRGVVSKVFPHLGYGFILKDGGGEVYFHKNAVKGIPFNDMEDGQEVLFE
                     SEPGEKGLHATIVQPPHALEV"
     gene            1298..3712
                     /locus_tag="PKK81_09415"
     CDS             1298..3712
                     /locus_tag="PKK81_09415"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV33138.1"
                     /translation="MSDKQKLPASLLAPQVDPGRLGFDDTSEVEPLDDTIGQERAVEA
                     LDFGLQMKSPGFNMFVSGPVGTGKGTLVRQMVKRLAQTAPAPFDWCYVHNFQDASRPT
                     CLSFPAGQGASFKREMSTFIDGLRRDIPAAFEGKKYLDAKAKIIEETEGKKKALFHDL
                     TALCHQRGFGFEETPVGFGLVPLKDDRPMTEKEMEALSEQVQQELTEKRQSLESDLRE
                     FHVRMHTLEREAEQALHHLDHQIVANVMQGAYETLQRAYQDLRPVTTYLQRVHHDIIH
                     QYKDFLPHSGPVLPIPGLEQTRRPDMTRFAVNLIVAREATAGAPVVDEPHPTYSNLIG
                     KIERRAHLGVMYTDFTEIRAGAMLQANGGYLILQALDLLRQPFSWDALKRVIKTGAVT
                     IEDPAEFYGFATAGLRPEPIPVSVKVILVGTAGIYHLLQAYEEDFSKLFKVKADFDVE
                     VPHDERQERQYARFVAKLCKEEGLPHFGADAVAEVIRQGFRYADRHDRLSLRFSLVSD
                     LIREAGYWARKDGHSFVTRADVESALAHQRHRADLPEQWIQDEIREGTLMVDLQGEVV
                     GQVNGLSVYELGDYAFGRPTRITARTYVGTKGVIDIQREVELAGNIHSKGVMTLAGFL
                     AGKFAGMQPFALSATLTFEQTYSEIEGDSAAVAELTAVLSSLADLPVRQSLAVTGSVN
                     QLGEIQPIGGVNEKIEGFFESCKRRGLTGTQGVIIPARNTKHLALNREVVAAVEEGKF
                     AVYGVDHVEQALELLIGVPAGERGPEGTFPPESIYGRTAARLEEMAQIVANWGEGEEE
                     PEKITG"
     gene            3729..3893
                     /locus_tag="PKK81_09420"
     CDS             3729..3893
                     /locus_tag="PKK81_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV33139.1"
                     /translation="MPMYDYTCLDCGKESLIIVTLKQHEKGEVQCPACGSTKLQQHFS
                     SFIAHTTKKS"
     gene            3911..4291
                     /locus_tag="PKK81_09425"
     CDS             3911..4291
                     /locus_tag="PKK81_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV33140.1"
                     /translation="MQIVMTTGTRYSFVVSLVVTLAAACGPATAQDFPPDVTRGKAVY
                     ERHCLACHGRGGWGDGPDAAALTVPPANFHRFKSFLKSDEELLRTIEHGIVFSAMHSW
                     QGQLTETERLDVLAYIRLLVQQGK"
     gene            4343..4924
                     /locus_tag="PKK81_09430"
     CDS             4343..4924
                     /locus_tag="PKK81_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNV33141.1"
                     /translation="MTYLTPHDALVVVDVQRDFLPGGALGIQDGDRIVPILTRYVARF
                     HARGLPIFITRDWHPPDHCSFREQGGPWPVHCIAGSPGSLPPPNFQPPVSAVTIYKAI
                     DQDKEAYSTLQDTPLDRHLRALKVRRLFIGGLATDYCVLNTVKDARALGYDVCVLMDG
                     IKAVNLHPDDGRRAEEAMVLAGAIPLCLERLAA"
     assembly_gap    5067..5166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5167..6128
                     /locus_tag="PKK81_09435"
     CDS             <5167..6128
                     /locus_tag="PKK81_09435"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNV33142.1"
                     /translation="VMNLLNFQTMVASKACRSVLVAGDKPLIDFGLRRAHGAEAGLLA
                     ARAAYLAGLAGTSNVSAGARFGIPIYGTMAHSFVQAHLDESEAFIHIAEAQPRNVVLL
                     IDTYDTEAAAEKVVSLASRLNARGISIHGVRLDSGDLADHARKVRHILDRGGLTATQI
                     LASGNLDEERIQTLVTGQAPIDSFAIGTAMTTSADAPYLDCAYKLQEYAGRPCRKRSE
                     GKATWPGRKQVYRRIGTDGRLNGDIITVEGDCREGQPLLGHVMQGGQRVASPPSLEDV
                     RRYARLSLTQLPAPLRRIDRADPYDVRIAPALTELAEQVDLHT"
     gene            6196..7626
                     /locus_tag="PKK81_09440"
     CDS             6196..7626
                     /locus_tag="PKK81_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNV33143.1"
                     /translation="MNQHSVSSTIVAGWVVAALLSLPTGADAAPKPAPKPSSGNTCES
                     AFKQTRPASKILDKVLQSHARWLEDRDSPDGRRANLCRTDLRQLRLVGANLERINLEG
                     AILKGSNLRTASLVQAHLKGADLSQAVLDDANLEGADLRKALLVKAHLNRIAADEAAF
                     YGANLQGALFREALLERAHFEDADLQGADLSNATLLDGYFYGANLSKANLTDADLAGT
                     DLRRTNLRQANLRRANLQGALMDSANLDGASLVEADLESAYLDDASLAHADLHEASLR
                     GADFRFTHLGGANLQRANLENANMEGANLVKARLDSATLTMTVLYKANLSAANLHGAS
                     LHHAVLIGTQLARADLRKADLTEIYGPNAHLQQAWLSEANLELANLVAADLSQADISH
                     AVVVQTNLQETNLRGANLSASDLTGALLNNADLGQADLRGANLSGALGLVQAQLDAAC
                     LDEATRIPSELQRPKPCAQPRPRLRK"
BASE COUNT         1660 a         2564 c         2312 g         1385 t
ORIGIN      
        1 cggtccgctt ccggtcgagc gcagtcggct tggtcgcccc tgccatgcgt tcgagatcct
       61 tgtgataggt cgctccctct gaccattcgg taagtaagaa aaactctttg gccgtagcca
      121 cggacatcaa ttcgccccgc gcggtgaaag cccccacatc cagcgcgtcg atatgtcccg
      181 gcaggcggcc gtagcagtca tagtcccaca acatcgcctg agcccggtcg gccggatgtt
      241 catggccgaa tggaccggga ctcatcgtgc ccaacaccac ccggcgggtc ttgcctccct
      301 ggcggaaagt caaccggaca ggcgcgccat agccatattg cttataccgc gcgccgctcg
      361 tctccttgcc gatcggtcca taggcgagca attccgcgct cggcccgaac cggaccttga
      421 ggtaggtgtc cagggcagat ttcttcaaca ccggcatacc gctgtccgac gcaagacatg
      481 ttcccgaaaa tatgcccgct tcagcctgca atggaaccag caactggcgc attgagcttc
      541 agcgcctcct gaggcagtga ggaattatgc gccagtgccc cgaaaggcca ccatcatccc
      601 gtctgctgaa gtcgcgcaaa tgccccactt tcagccggtt cagctccctc cccgccccgc
      661 gttcccatct gcggcaaggg catgtgcttt gctgagacag caggtgcaca gactgttcat
      721 ctgagacgag aggaggccgc atgaacctgg aagttgagag ccgcaatatc gccatgaccc
      781 cgcgctggaa aaccgagatc gaagagcgga tggccacctt gcagcgtggc cacgacgaca
      841 tcatccatgg gcgcgtcacc ctgacgaaga atcgccatca taaaaaactg gacaacgtgg
      901 cagaagcgct tgtgctggtc acggtgccga cccgtcacac catcacctcc cgcaaggagg
      961 acaaaacttt cgaggaagcg atccgggccg ccttcgacgc cgtggcgatc gaactgcgga
     1021 aatatcgcga gaagcgggcc gacaaagttg tgcgcatcga accgctgccc caactccgcg
     1081 gtgtggtgag caaagtattc cctcacctcg gctacggctt catcctgaag gacggcggcg
     1141 gcgaagtgta tttccataag aatgccgtga agggcatccc attcaacgac atggaggacg
     1201 gacaggaggt cctcttcgaa agcgaaccgg gcgaaaaggg cctacacgcc accatcgttc
     1261 aaccacccca cgcgctggaa gtgtaggagt ccggacgatg tcggacaaac agaagcttcc
     1321 cgccagcctg ttggctccgc aggtggaccc cggccgcctg ggattcgatg atacgagcga
     1381 ggtcgaaccg ctcgatgaca cgatcgggca agagcgggcg gtcgaggcct tggacttcgg
     1441 ccttcaaatg aagagccccg gcttcaacat gttcgtctcc gggccggtcg ggactggcaa
     1501 gggcacactc gtcaggcaaa tggtgaagcg gctggcccaa acggcaccgg ctccattcga
     1561 ctggtgttac gtccacaact ttcaggacgc gtcgcgcccg acctgccttt cgtttccggc
     1621 cgggcaaggc gcgtcgttta aacgcgagat gagcaccttc atcgatggcc tccgccggga
     1681 cattcccgcg gccttcgaag gcaagaagta cctcgatgcc aaggcgaaga tcatcgagga
     1741 gacggaagga aaaaagaagg ccctcttcca cgacctcacc gcgctctgtc accagcgcgg
     1801 attcgggttc gaagaaacgc cggtcggctt cgggctggtc cccctgaaag acgaccgccc
     1861 gatgacagaa aaggaaatgg aagcactctc tgaacaggtg cagcaggaac tcacggaaaa
     1921 acgacagtcg ctcgagagcg acctgcggga gtttcacgtc cggatgcaca ccctcgaacg
     1981 ggaggccgag caggcgctgc atcatctcga ccaccagatc gtcgcgaacg tcatgcaagg
     2041 ggcctacgaa acgctgcagc gggcctatca agatctacga cccgtgacga cgtacctgca
     2101 gcgggtccat cacgacatca ttcaccaata caaagacttc ctgcctcaca gcggtccggt
     2161 gctgccgatc cccgggctgg aacaaacccg tcgcccggac atgacccggt tcgccgtcaa
     2221 cctcatcgtg gcgcgggaag ccaccgccgg agcaccggtt gtggatgaac cccacccgac
     2281 ctacagcaac ctcatcggaa aaatcgagcg ccgggctcac ctcggagtga tgtataccga
     2341 tttcaccgaa atccgggccg gggccatgtt gcaggccaac ggcggctact tgattctgca
     2401 ggcgctggat ctcctccgcc agccgttttc ctgggacgcg ttgaagcgcg tcatcaagac
     2461 cggcgccgtc accatcgagg acccggccga gttctatgga ttcgccaccg cgggcctgcg
     2521 gcccgaaccg attcccgtct cggtcaaagt catcctggtc ggcacagcgg gaatctacca
     2581 cctgctgcag gcctacgaag aggatttctc gaaactcttc aaggtgaaag ccgatttcga
     2641 tgtggaggtc ccgcacgatg aacggcagga gcggcaatat gcgcgcttcg tcgccaaatt
     2701 atgcaaagag gaaggactgc cgcatttcgg cgccgacgcc gtggcggaag tcatccgcca
     2761 ggggtttcgc tatgccgacc ggcacgatcg gctgtccttg cgattcagcc tggtcagcga
     2821 cctcattcgc gaagccgggt attgggccag gaaagacggg cactccttcg tgacgcgcgc
     2881 ggatgtcgaa tcggccctgg cgcaccagag acaccgtgcc gacctacctg agcaatggat
     2941 ccaggatgaa atccgcgaag ggaccttgat ggtggatctg cagggcgagg tcgtcggtca
     3001 ggtcaacggg ttgtccgtct atgagctggg cgactatgcc ttcggccgtc ccaccaggat
     3061 caccgcccgc acctatgtcg gcaccaaggg cgtgatcgat attcaacgcg aggtcgagct
     3121 cgcgggaaac atccacagca agggggtcat gacgctggcc ggattcctgg cgggcaaatt
     3181 cgcggggatg cagccgttcg cgctcagcgc caccctcacc ttcgaacaga cctactccga
     3241 aatcgaggga gacagtgccg ccgtggccga actcacggcc gtcctctcaa gcctcgccga
     3301 ccttcccgtc cggcaatcgc tggccgtcac cggctcggtg aaccaactcg gtgaaatcca
     3361 acccatcggc ggggtcaacg agaaaatcga agggttcttt gagtcatgca agcggcgcgg
     3421 cctgacaggc acgcaagggg tgatcatccc cgctcgaaac acgaaacacc tggcactgaa
     3481 ccgggaagtc gtcgcggccg tggaagaggg aaagtttgcc gtctatggcg tggaccacgt
     3541 cgaacaggcg cttgagctgc tcatcggcgt ccccgccggg gaacgcggac cggaaggaac
     3601 cttcccgcct gagagcattt acggtcgaac cgccgcgcgg ctggaggaga tggcgcagat
     3661 tgtcgcgaat tggggagaag gcgaagaaga gccggaaaag atcacggggt aaacagaacg
     3721 aggaccctat gcccatgtac gactatacct gtctggactg cggaaaagaa tcgctgatca
     3781 tcgtgacgct gaagcaacat gaaaaaggcg aggtgcaatg tcccgcctgc ggcagcacca
     3841 aactgcagca acacttttcg tcgttcatcg cgcacacgac caagaaaagc tgaaggagac
     3901 acagagactc atgcagatcg tcatgaccac cggcacccgg tattccttcg tcgtctcgct
     3961 tgtcgtgacc ctcgcggccg cctgtggccc ggcgacggca caggacttcc cgccggatgt
     4021 cactagaggg aaggctgtct acgagcgtca ttgcctcgct tgccatggcc gtggcggatg
     4081 gggcgacgga cccgatgccg ccgcattgac ggtcccgccg gccaacttcc accggttcaa
     4141 gtccttcctc aagtccgacg aggagttgct ccgaacgatc gagcacggca tcgtcttcag
     4201 cgcgatgcat tcctggcagg ggcaactgac cgaaacggag aggctggacg tgctggccta
     4261 catccgcctg ctcgtgcagc agggaaaatg acgcagcgcc ccgccggctg cgagccgatg
     4321 ccgtatggac gggacatgct caatgactta cctcacccct catgacgcct tagtggtggt
     4381 cgacgtgcaa cgggatttcc ttccgggcgg cgcattgggc atacaagatg gcgacaggat
     4441 cgtcccgatc ctgactaggt acgtggcacg atttcatgct cgagggctac cgattttcat
     4501 aactcgcgac tggcatccgc ccgaccactg ttcgtttcgg gagcaaggcg ggccttggcc
     4561 ggtccactgt atcgcaggat cgcccggctc cttgccgcca ccgaacttcc agcctcccgt
     4621 ctcggcggtc acaatctaca aagcgatcga tcaggacaaa gaagcctatt ccacattgca
     4681 ggacacgcca ctcgatcgtc atttacgcgc cctgaaggtc cgccgcctgt tcatcggggg
     4741 gttggcgacc gattactgtg tgctgaacac ggtgaaggat gcgcgggcgc ttggttacga
     4801 tgtgtgcgtg ctcatggatg ggatcaaggc cgtcaatctc caccccgacg acggccggcg
     4861 cgccgaagaa gcgatggtgc tggccggcgc cattcccctt tgcctggagc gattggccgc
     4921 atgaacatca cctcgggcgc gctgctcacg gacctctacg aactcacgat ggcgcaggcc
     4981 tatctcgccc agggaatgac ggacatcgcc gtctttgaat tctttgtccg gaaactgccg
     5041 ccgcaacgca acttcttcat ggccgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnngggt catgaatctc ctgaacttcc aaacgatggt ggcctccaag gcctgccggt
     5221 cggtcttggt tgccggcgac aaacccctga tcgatttcgg cctgcgccgc gcgcatgggg
     5281 cggaagcggg ccttctcgcc gcccgcgccg catacctcgc cgggctcgcg ggcacgtcga
     5341 acgtatcagc cggcgcccgc ttcggcatcc ccatctacgg gacgatggcc cattcgtttg
     5401 tccaggctca tctcgatgag tcggaggcct tcatccacat cgcggaggcg caaccacgca
     5461 acgtcgtgct cctcatcgac acctatgaca cggaagccgc ggcggagaag gtcgtctccc
     5521 tggcgagtcg cttgaacgca cgaggtatct cgatccacgg cgtccgactc gatagcggag
     5581 atctggccga ccatgcgcgc aaagttcggc acatcctcga tcgcggaggt ctcacggcca
     5641 cccaaatcct cgccagcgga aatcttgacg aggaacgcat ccagacgctg gtgacaggac
     5701 aggcaccgat cgacagtttt gcaatcggca cggccatgac cacatcggcc gatgccccct
     5761 acctggactg tgcctacaaa ctccaggaat atgccggcag accctgccgc aaacgttccg
     5821 aaggcaaagc cacctggccg ggtcgaaaac aggtctaccg ccggatcgga accgacggac
     5881 gcctgaacgg tgatatcatc acggtcgagg gcgactgccg ggaaggccag cccctgctgg
     5941 gccacgtgat gcagggaggt caacgcgtcg catcccctcc gtcgctggaa gacgttcgac
     6001 gatatgcccg gctctcgttg acacaactgc cggccccgtt gcgccggatc gaccgcgccg
     6061 atccttacga cgtccggatc gcgccggctc tcaccgagtt ggcggaacag gtcgatttac
     6121 acacatgagc cacatgatgc tcccggaacc atgccattca gcacagcacc gaatcttctt
     6181 gaaggaggcc cgttcatgaa ccagcattct gtttccagca ccatcgtcgc aggttgggtg
     6241 gtggccgcct tactctccct gccgaccggt gctgacgccg cgcccaagcc ggcgcccaaa
     6301 ccctcctcag gcaatacctg cgagagcgcc ttcaagcaga cccgtccggc gtcaaaaatt
     6361 ttggacaagg tgctccagtc ccatgcccgc tggctggaag accgagactc ccccgatggc
     6421 cgccgggcca atctctgccg caccgacctt cgacaacttc gcctggtagg agccaatctc
     6481 gaacggatca atcttgaagg ggcgatcctg aaaggctcga atcttcgcac cgcgagtctg
     6541 gtgcaggcgc acctcaaagg cgcggacctc tcccaggctg tactggacga tgccaatctt
     6601 gaaggcgccg atctgcgcaa agccctgctg gtgaaggccc atctcaatcg catcgccgcc
     6661 gacgaagccg cgttctacgg agccaacctg caaggcgcgc tcttccggga ggcgctgctg
     6721 gagcgggctc attttgagga tgcggatctg cagggcgcgg acctcagcaa cgccaccctc
     6781 ctggacggct atttttatgg cgcgaatctg tcgaaggcca atctcaccga cgccgacctc
     6841 gccggaaccg acctccgccg gaccaatttg cgccaggcca atttacgccg ggccaacctg
     6901 cagggtgcct tgatggacag cgccaacctc gacggggcat ccctcgtcga agcagatctg
     6961 gagagcgcct acttggacga tgcctcgctg gcgcatgcgg acctccatga ggccagcctt
     7021 cgtggcgcgg attttcgctt tacgcatctt ggcggcgcca acctgcagcg agccaatctt
     7081 gaaaatgcga acatggaggg agccaacctc gtgaaagcca ggctggactc ggccacactc
     7141 acgatgaccg tgctgtacaa ggccaacctg tcggccgcca atctgcacgg ggccagccta
     7201 catcatgccg tcctcatcgg gacgcaactc gcgcgggccg atctccggaa agccgacctc
     7261 accgaaatct acggcccgaa cgcccatctg cagcaagcct ggctcagcga agccaatctg
     7321 gaattggcga atctggtggc cgccgacctg agtcaggccg acatcagcca cgccgtcgtg
     7381 gttcaaacca atctccagga aaccaatctg cgcggagcca atctcagtgc gtccgatttg
     7441 acgggagccc tgctgaacaa cgccgatctt ggccaggccg acttacgagg cgcgaacctg
     7501 agcggcgcgc tggggctggt tcaagcacag ctggatgcag cctgcctcga tgaggcgacc
     7561 cggattccgt ccgagctcca gaggccgaag ccctgcgcac aaccacgccc gcggctacga
     7621 aaatagcgta cccagcgaac cctcaacgta agggtcgccg acccggccgt acgtacgcag
     7681 cctctggcgg aatgttcatc acacgctcgt tgctgtgggg ctgcggtacg gacggtacca
     7741 ggcggatcga ttcgatgtcg tgggagcagt gatctgcctc gtcggcaggg cggtcatcat
     7801 gtacatgcct cgtcccccga tgtaagccat cgttcaccat cccggttcac gcattccaac
     7861 cactccaccc agacaacagg catttcccca ctgaccggct gcacatcgac cggtctgatg
     7921 ggagaggctc gctgtcatat caactacata gatcgttgat ccgtgcgcag acgagctacc
     7981 ttgcctactt cacacttagg gtttaagccg tccctaacta g
//
LOCUS       DAOQRM010000114         7832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_8901_length_7832_cov_16.480777, whole genome shotgun sequence.
ACCESSION   DAOQRM010000114 DAOQRM010000000
VERSION     DAOQRM010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8901_length_7832_cov_16.480777"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..828)
                     /locus_tag="PKK81_09445"
     CDS             complement(145..828)
                     /locus_tag="PKK81_09445"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNV33144.1"
                     /translation="MSKKEIDAARRLMSLMFKAHPWHGVSMGDHAPDLVTAYIEIVPT
                     DTVKYEVDKASGFLKVDRPQRFSNYCPSYYGLIPQTYCGERVAELFGARAGRPDMIGD
                     GDPLDICVLTEKTIPHSDILLTARPIGGFSMADGGEADDKIIAVMRDDAVYGSFTDLK
                     ECPAALLDRLHHYFLTYKHAPGSTQHKVEITSIYGRDEAMKVIHASHDDYKQKFPELE
                     FMWPASMNS"
     gene            1385..>1617
                     /locus_tag="PKK81_09450"
     CDS             1385..>1617
                     /locus_tag="PKK81_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV33145.1"
                     /translation="MSTRAVTVTMDDSLARARDLFNEHHFHHLLVVQGPTLLGIISDR
                     DLLKAVSPNIGTLSETDRDRATLNKRAHQIMSR"
     assembly_gap    1618..1717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1718..2286)
                     /locus_tag="PKK81_09455"
     CDS             complement(<1718..2286)
                     /locus_tag="PKK81_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNV33146.1"
                     /translation="MRRNREQFVFGGAMVLILVMSLAPAFSQAAEWVHEGMAEEGKVT
                     MGFRVGPSFTTQSAGVSTVGPALNFQGMYGLNKWFRLGMMLEWESHGIDGPRSGSVDT
                     ITLLPVNLEYRPGHFGNFIPYFTTGLGVNINTKDVKDTFAWRIGGGIDYALTNLVQGA
                     PKGLMLNMEAAWKRNHPDSDGSTMGLLFGV"
     gene            complement(2670..3323)
                     /locus_tag="PKK81_09460"
     CDS             complement(2670..3323)
                     /locus_tag="PKK81_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33147.1"
                     /translation="MQKLVRVLPVLMLTLSPIACAHSADSPATAVSPSATAYPAKVAE
                     TKAVLRDLWLGHILSIRNVAVATMDKNASARSAAEAGVVANAEQIARSIEPFYGKAAS
                     DKLFTLLAGHYGAIRDHLDATVAGAASRQEAAVKALTANAAEISTFLSGANPNLPKDT
                     LMGLLMAHAAHHLTQFQQLKDGDYIHEAETWTGMKQHIYVVSDALAQALAAQFPAKF"
     gene            complement(3457..4188)
                     /locus_tag="PKK81_09465"
     CDS             complement(3457..4188)
                     /locus_tag="PKK81_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNV33148.1"
                     /translation="MMYTAMNMTDHRQCVALSGCFRGKLCDQLMHRPGRSVGKGELVY
                     GLGDSAQSVFFLRRGFVKLTSLTEDGRELILRLHQAGEVFGELCHCTGERREQAVAME
                     DSEIVELNFDEFVAHLQNNRPAFLLFLSNVAQQLSAAYDQIQTLSFSSTMERLVRTLG
                     RLADEFGEPDGEWVRLTHYFRQDDLAQMIGARREVVSTLLNQLRDRGLINYARKDGLL
                     LHRAGLEGFLGTAEKSPANLSDSGF"
     gene            4354..5169
                     /locus_tag="PKK81_09470"
     CDS             4354..5169
                     /locus_tag="PKK81_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV33149.1"
                     /translation="MPTSSHQRPTRRGRPGIALIGIVCLATSLIAASLLWAGSPSQTK
                     PGNGPADAEQGRAIFNGKGLCSTCHGIDGYRDRLPPQLSKNIRDNVEHLSPAPPDLRQ
                     AATLTLTTDKQRFEVIRHGHLRTAMYPLSKEALSDEEILALLPYLASIRGTVPPPAGL
                     PEPDTSLRGDAKSGRQLYHELGGCAICHGIEGHLNRRPPMSADLIKRLDALPAPPANL
                     RDSATLKSQNDEDRYRSIKRGHPGTAMYPKTLLRDEDIRDLVAYLATLRETGR"
     gene            complement(5398..5790)
                     /locus_tag="PKK81_09475"
     CDS             complement(5398..5790)
                     /locus_tag="PKK81_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33150.1"
                     /translation="MTEVNSMYRAQTRSDDLKAQIEKLEGLAHHTAVNPTFERFDAET
                     EDILMKIYGENHKYVESYKYATVGEAEALVNLPESAQEPQTRDIPKKGLQQRRQVLQS
                     ILTDLQGLEVQEAELLTGEDREDPPGPS"
     gene            complement(5903..6376)
                     /locus_tag="PKK81_09480"
     CDS             complement(5903..6376)
                     /locus_tag="PKK81_09480"
                     /inference="COORDINATES: protein motif:HMM:NF021425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HNV33151.1"
                     /translation="MMPFMTPPSPRGGLALIAVFKLLKGVVLLLVGAALLRLVDPEIA
                     TFLAPAMDLLHLHGHSRLVHSLLLTVSALSSHSVFLMAYASLLYSVFLLIEGCGLWLE
                     ASWAGYMAVISTSVFLPVEFYDVLRQISVVHVTMLLFNVAIVGYLVAQLERRSLR"
     gene            6509..6868
                     /locus_tag="PKK81_09485"
     CDS             6509..6868
                     /locus_tag="PKK81_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNV33152.1"
                     /translation="MNEQQATRLAQAWIDAWNRHDPDAILRHYAPDVEFTSPFVSALS
                     GEASGTICGREALSAYFRKGLQAYPDLHFELIRTLIGVDSLLLYYRSVTGLLAAEMMI
                     INGEGQIHTVRVHYVKE"
     gene            complement(6953..7456)
                     /locus_tag="PKK81_09490"
     CDS             complement(6953..7456)
                     /locus_tag="PKK81_09490"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNV33153.1"
                     /translation="MVILVMGVSGAGKTTIGRRLADELGWQFSDGDDFHPAANLEKMR
                     NGHALTDVDRQPWLERMHAAIVDRISRNQPAVLACSVLKASYRAIVEEGCRAHLRLVY
                     LKGSFDLFHQRLIHRRDHFMPRELLASQFAVLEEPADALVIDAALPPNEIIRQIRLGI
                     PVDAAKH"
     gene            complement(7524..>7832)
                     /locus_tag="PKK81_09495"
     CDS             complement(7524..>7832)
                     /locus_tag="PKK81_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33154.1"
                     /translation="AEVAWTQGNAGKARVCARRAVALAAAAWWARSGRSLRHGDALEH
                     LRRIQQQEAFPSPVQQAAERLTTAVTKRHDAPFTSDPIGDARLIVGYLAAATTDRRLA
                     "
BASE COUNT         1677 a         2448 c         2157 g         1450 t
ORIGIN      
        1 agcccaaggc ttccagggtg gtcaacagcg aggcttctaa tccgagcgcg gcaaaaccgg
       61 gagagaatcc cttggccgtc ggctctgtct gcgaatgcgt cgtggtgtgt ttcatcaggg
      121 ggggataaac ctttcttggt agtactagct gttcatgctg gcgggccaca tgaattctaa
      181 ctcggggaat ttctgtttgt agtcgtcgtg actggcgtga atgactttca tggcctcatc
      241 gcggccgtag atgctcgtga tctcgacctt gtgctgcgtc gatcccggcg cgtgcttgta
      301 ggtcagaaaa taatggtgga gccgatccag gagtgccgcc ggacattctt tgagatcggt
      361 aaacgacccg tagacggcgt cgtcccgcat gaccgcgatg atcttgtcgt ctgcctcgcc
      421 gccgtctgcc atgctgaatc cgccgatggg ccgagccgtc aacagaatat cgctgtgcgg
      481 aatcgtcttc tcggtcaaca cgcagatatc gagcggatcg ccgtctccga tcatgtccgg
      541 ccggcccgcc cgcgcgccga acagttccgc tacccgctcg ccgcaatagg tttgcggaat
      601 cagcccgtaa taggacgggc agtagttgga aaatcgctgc ggacggtcga ccttgagaaa
      661 cccgctggcc ttgtccacct catatttgac cgtatcggtc ggcacaattt cgatataggc
      721 cgtcaccaag tcaggcgcat ggtcgcccat ggacactccg tgccacggat gcgccttgaa
      781 catcaggctc atcagtcgcc gcgctgcatc gatttccttc ttgctcatcc taattcattc
      841 cccctgtgaa gcccccggaa ctgacggtct gacgatcgtg cgatccccga aggtctggat
      901 tgattctacg aaccactgca ctgaaccatg attgatcggc gagagaaaca gacagcttat
      961 aacacatcgg ggccgaatga aacagcaggg agtctgcggt cggctcccgc gccgacgaca
     1021 gaccgacttc gccatcaagc cgaaccatca ggaactcccg ccctgcggat acacatgacg
     1081 agcagaaaca cctcagccga acgagaaact ggacgcccgg gcgagaccgt cccgcacaac
     1141 atccattcgc aatcggccat tcgatccggc cttccagccc cgcgcgcaag tcgcgccaaa
     1201 ctctcaagaa tggtctcccg cccgccgata agggaagcag agcgggaagg cagaacgaca
     1261 cgcgcgttcc aattcctgcc catgaggacc gtccatgtcc caatcaggtc ttaatgattc
     1321 cgcgaaaatg cctcccccgg tcgtcccctc cgacaccatc gcacagccga cagtacgtgc
     1381 catcatgtcg acacgagccg tcaccgtcac catggacgat tccctcgctc gagcgcggga
     1441 cctgttcaat gaacatcatt ttcaccacct gctggtcgtc cagggcccta cgctactcgg
     1501 catcatttca gatcgcgacc tgctcaaggc ggtgagtccc aatatcggga cactgtccga
     1561 aaccgatcgc gaccgggcga cgctcaacaa acgcgcccat caaatcatga gccggaannn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca ccgaagagca ggcccatggt
     1741 ggacccgtcg ctgtcgggat ggttacgctt ccaggccgct tccatattca acatcaggcc
     1801 tttcggcgcg ccctgaacca gattggtcaa ggcatagtcg ataccgccgc cgatccgcca
     1861 ggcgaacgta tctttcacat ccttcgtatt gatgttcacc cccagaccgg tggtgaagta
     1921 cgggatgaaa ttaccgaaat gtcccgggcg atactccagg ttcaccggaa gcagcgtaat
     1981 ggtatcgacg gagccgcttc gaggcccatc gatcccgtga ctttcccact ccagcatcat
     2041 cccgagacgg aaccatttgt tcagtccgta catgccttga aagttcagcg ccggaccgac
     2101 cgtcgacaca ccggcacttt gcgtggtaaa gctcggtccc actcgaaacc ccatcgtgac
     2161 tttcccctct tcggccatcc cctcatgcac ccactcggca gcctgagaga aggcgggggc
     2221 caaactcatc accaggatca gcaccatggc cccaccgaac acaaattgct cacgatttct
     2281 gcgcataccg tcctcctatg ataatgaaac catcttcaag cgatgcactc taggcaagtg
     2341 gccgttgcgt tttcaagaaa aagcgtgcgg ttccggcgaa acagaatggt tccaattgtt
     2401 gtcgcaaact gagacggaat gaccgctcaa ataggaggcg cagagaaaac cactagtacg
     2461 tagtattgag cagttgtacc ggaacgatct cccgccctga gcgagcagaa gcggagggcc
     2521 ggaagggaga gggccgaacg agatcgaccg gcgtgaggaa gtcacgtcga ggagggcagc
     2581 caggtggcgg ggtctccgct cctggctgcc tgaatccggc ctccgaaaat aatcagaaac
     2641 gagacacagt caatgcgcga atagtcctcc tagaacttcg cagggaattg cgccgctaac
     2701 gcctgcgcca gcgcatcgga caccacgtag atgtgttgct tcatacccgt ccaggtctcc
     2761 gcctcatgta tgtagtctcc gtctttcaac tgctgaaact gcgtcaggtg atgcgcggca
     2821 tgggccatga gcagtcccat caacgtatcc ttcggcaaat tcgggttggc gccgctgaga
     2881 aacgtcgaaa tctctgccgc attcgcggtg agggccttca cggccgcctc ctgccggctc
     2941 gccgcccctg ccactgtcgc atccagatga tcgcgaatgg cgccgtagtg accggccaac
     3001 aaggtaaaga gtttatcgga cgcggccttg ccgtaaaacg gctcgatgga ccgcgcaatc
     3061 tgctcggcat tggcaaccac acccgcttcg gccgcgctcc gcgcagacgc attcttatcc
     3121 atcgtcgcga cagcgacatt gcgaatcgag aggatatggc ccagccagag atcccgaagc
     3181 acggccttag tctccgccac cttggccggg tacgccgtcg ccgacgggga caccgctgtt
     3241 gcaggcgaat cagccgaatg ggcacaggca atcggggata gagtcaacat caacacaggc
     3301 aacactcgaa ccagcttctg catggcaact ctcctttaga aaatagtaat ggcgcgccgg
     3361 atcaaccgac accggcggta gcatcgagcg gcaaggatag agaacgggag cagttctgta
     3421 agttagcggg ataacattgc ggggagaaac gtggtcttag aagccggaat cgctgagatt
     3481 ggcaggagac ttttcggcgg tacccagaaa tccctcgagc ccggcacgat gcagcagcag
     3541 cccgtctttc cgcgcgtaat tgatcaggcc ccggtcgcgc aactggttca agagggtcga
     3601 gaccacttcc cggcgcgcgc cgatcatctg cgccagatcg tcctgccgga aataatgcgt
     3661 gagccgcacc cattcgccgt ccggctcgcc gaattcatcg gccaaccggc ccagcgtccg
     3721 caccaggcgc tccatggtgc tgctgaacga gagggtctga atctgatcgt aggccgccga
     3781 cagctgctgc gcgacgttgg agagaaacag caggaaggct ggacgattgt tctgcagatg
     3841 agccacaaat tcatcgaagt tcagttcgac gatctcgctg tcttccatcg ccacggcctg
     3901 ttcgcggcgt tccccggtac aatggcagag ctcgccgaac acttccccgg cttgatgcag
     3961 gcgcaaaatt aattcacggc catcctcggt cagagaggtc aacttgacga atccccgccg
     4021 aagaaagaag acgctctgcg cggaatcgcc gaggccgtag acgagttccc ccttgcccac
     4081 agaacggccg ggtctgtgca tgagctggtc gcacagcttg ccgcgaaaac agccagacag
     4141 ggctacgcat tgacggtggt ctgtcatatt catcgccgtg tacatcatgt gtccctcact
     4201 gctgcgcatt gtaccggcat cctgaagcgc gtgaccagac ccccgcaggg ggcagcatga
     4261 ctctgctcca taatcaggca tggcactgca cctccttcct cgattatgat ggagtgaaac
     4321 aggtccgaca ggcaggctac cgagacactg accatgccga catcctccca tcagcgcccg
     4381 actcgaaggg gccgccccgg aattgccctc atcgggatcg tctgcctcgc gacctcattg
     4441 attgccgctt cacttctctg ggcaggatcg ccgtcacaga ccaagccggg caacggaccg
     4501 gcggatgctg agcaaggccg ggccatcttc aacggcaaag gcctctgctc cacctgccat
     4561 ggcatcgacg gctacagaga ccggttgccc ccgcaactga gcaagaacat ccgcgacaat
     4621 gtcgaacatc tctcgcctgc gccacccgac ctgcgacagg cggccacgct caccctcacc
     4681 accgacaaac aacggttcga ggtcatccgg cacgggcatc tccgcaccgc catgtacccg
     4741 ctctcgaagg aagccctctc cgacgaagag attcttgcgc tgctccccta cctggcctca
     4801 attcgcggca cggtgccgcc acccgccggc ctacccgaac ccgatacctc gctgcggggt
     4861 gatgccaaaa gcggacggca gctctatcat gaactcggtg gatgcgcgat ctgccatgga
     4921 atcgaaggac atttgaatcg aagaccgccg atgtctgccg acctgatcaa acggcttgac
     4981 gcgcttccgg cgccgcccgc gaacctccgg gattccgcca cgctgaaatc gcagaacgac
     5041 gaggaccgct accgctcaat caagcgcggc caccctggca cagccatgta tccaaagaca
     5101 ctcctccggg acgaagatat ccgcgacctc gtcgcgtacc tcgccaccct tcgtgaaaca
     5161 ggccggtagt tccccttcct tgcatcgcgc cgatgtagag gggacgggac tgggcctggc
     5221 tattccctcg tcaatttaac tcgcgcgaat cgcgggatat gaagatggaa taccagaaga
     5281 gaagcgccca cgagaagata gggtagagac cggctcgcgg aggcagcgac cgcattggat
     5341 ataaacggaa cggcggccac cacctccatg cctggacagc tgcgatgcac ggtgagatca
     5401 gctgggaccg ggaggatctt cgcgatcttc tccggtgagc aattcggctt cttgcacttc
     5461 gagtccctgc agatcggtca gaatgctctg caacacctgc cggcgttgct gcagcccctt
     5521 cttcggaata tctcgcgtct gcggttcctg ggctgattcg ggcagattca ccagcgcttc
     5581 ggcttccccc accgtggcgt acttatacga ttccacgtac ttatggttct cgccatatat
     5641 tttcatgagg atgtcttccg tttccgcatc gaaccgctcg aacgtggggt tcacggccgt
     5701 atggtgggcg aggccttcga gcttttcgat ctgggccttc aggtcatcac tccgcgtctg
     5761 cgcacgatac atggaattga cttcagtcat gacgtctcct cccggtttga ttcttttgta
     5821 cctcccttcg agcaggtcgg cagctaggca ttcccctgga cggtcaccgg ggctcgcatc
     5881 aacctggaac gaggcttcgg tcttaccgca acgaccgtcg ctccagttgc gcgaccagat
     5941 accccacgat ggcgacattg aaaaggagca tggtcacatg cacgaccgag atctgcctca
     6001 gcacgtcgta gaattcgacg ggcagaaata cactggtgga gatgacggcc atgtatccgg
     6061 cccacgacgc ttcgagccac aggccgcagc cttcgatgag caaaaagacg gagtagagca
     6121 ggctggcata ggccatcaaa aagacgctgt gggatgacaa ggcggagacc gtgagcagaa
     6181 gggagtggac caggcgggaa tggccgtgga gatgcaagag atccatagcc ggagccagga
     6241 acgtggcgat ctcaggatct accaatcgga gcagagcggc gccgaccagc aacagcacca
     6301 cgcccttgag cagtttgaat actgcgatca acgccaagcc gcctcggggc gagggcggcg
     6361 tcatgaatgg catcatgtct tgcccggcca tcctggctca atcggatcgt tctgcatcat
     6421 cgttatgcga acctcaacag actcttgatc atcgaaccgg ccgcgcctag gctaggccca
     6481 tcacccattg acgaaaggtc tgaccgtcat gaacgaacag caggccaccc gcctggccca
     6541 ggcctggatc gacgcctgga accggcacga tccggacgcc atcctgagac actatgcgcc
     6601 ggatgtggag tttaccagtc cgtttgtatc cgcgctatcc ggggaagcgt ccggcaccat
     6661 ctgcggccga gaagcattga gcgcctattt tcggaagggg ctccaagcct acccggacct
     6721 gcacttcgag ctgatccgca ccctgatcgg cgttgacagt ctgttactgt attatcggag
     6781 cgtcacggga ttgctggctg cggagatgat gatcatcaat ggagagggtc agattcacac
     6841 ggtgcgcgtc cactacgtca aggaataatc gggactggat ggaaaaggaa acgccacgat
     6901 cgcgttgtcg gaacgggaca catgatttcc ggcctcgcag cctgatccgt cctcagtgtt
     6961 tcgccgcgtc aaccggaata ccgagacgga tctgccggat gatctcgttc ggcgggagag
     7021 ccgcgtcgat caccagggca tcggccggct cttcaaggac ggcaaactga ctggccaaca
     7081 attcccgggg catgaaatga tcccggcggt ggatcaggcg ttgatggaat aaatcgaagc
     7141 tacctttcag gtagaccagt cgcaaatgtg cgcgacagcc ctcctccacg atcgcgcggt
     7201 aactcgcctt caagacggaa caggcaagca ccgccggctg gttccgactg atccgatcga
     7261 cgatcgcagc atgcatccgc tccagccagg gctgacgatc aacatccgtc agcgcatggc
     7321 cattccgcat cttctcgaga ttcgctgccg gatggaagtc atcgccgtcg gaaaactgcc
     7381 agcccaattc gtccgccaag cgacggccga tcgtcgtctt ccccgctccc gatacgccca
     7441 tgaccagaat gaccacaacc ctccacttcg ttcgacctgc ggctggacga gataggcgct
     7501 gttccacccg ccggggccaa ccgtcaagca agccgccggt ccgttgtagc cgcggcaagg
     7561 tagcccacaa tcaaccgggc gtccccgatc ggatcactcg taaagggcgc gtcatgccgc
     7621 ttggttacgg cggtggtcaa tcgctccgcc gcctgttgga caggcgatgg gaaggcctcc
     7681 tgctgctgaa tccggcgcaa atgctccagg gcatcaccgt gccggagtga gcggccagag
     7741 cgcgcccacc aggctgcggc ggccagcgcc acagcccgcc gcgcgcagac ccgcgccttg
     7801 cctgcattgc cttgtgtcca cgcaacttca gc
//
LOCUS       DAOQRM010000115         7830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_8903_length_7830_cov_13.467781, whole genome shotgun sequence.
ACCESSION   DAOQRM010000115 DAOQRM010000000
VERSION     DAOQRM010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8903_length_7830_cov_13.467781"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1546
                     /locus_tag="PKK81_09500"
     CDS             146..1546
                     /locus_tag="PKK81_09500"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNV33155.1"
                     /translation="MRGLMWRAATLVMLLLGTAGCADSLSGLSGSGVAKGTGQTVTVH
                     PREIDEVLDNPGMGFADFHFGFGHPPAPEEYPHTTVAYFRWPWAELEPKEGQYNFALV
                     DRVIEQAKAKGETLAIRIVSEYKTGSPQWLLDKGVGSVKESDGIFPDYNHPVFLDYHE
                     RLIRAFGERYGRSVDIDHVDIGSIGCWGEWNTVCCEGVEAQCKAFFPTEANQIAITDW
                     YLKYFAGTPLVMLHGGQLKYAASHGAGWRGDCFGDYGYFSPDWNHMEHAYPPVLEEAV
                     IANAWKRGPVQMEVCGYIHEWYDRGFDLDRILNQGLEWHLSVLNAKSKPVPAAWRPRF
                     NEFLKRIGYRFVLRELTHSAESHPGGPLVLQSRWENKGVAPIYHAWPLAYRLRSSSDQ
                     VVAQWTSPADLKQWLPGPSPRVEDTVVVPETLSAGSYALDVAILSEDALSAHVELAIE
                     GKRADRWYALSRVEIR"
     gene            complement(1622..2545)
                     /locus_tag="PKK81_09505"
     CDS             complement(1622..2545)
                     /locus_tag="PKK81_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV33156.1"
                     /translation="MLFPQTQLLLVDDSPLNLNILLEFLREKEYSCTTAQSGVQAWNM
                     LEREPDRFHAVLLDRIMPEMDGMEVLHRMKHHTVLSQIPVIMQTAASTHQQTLEGLQA
                     GAYYYLAKPFDKATLQAIVDAAVRDHISYLDVRQDLRRTTTTMRLLNSGTFRFKTPEE
                     AKNLAMLLAHAYPDANRVVTGILELTLNAIEHGNLNIGYKEKSRLIEEERLDNEIERR
                     LSDPLYSSREATAHFVRHPDKLSLHITDQGKGFEWRKYLNFDPERAFDTHGRGIAMAN
                     KVSFDRIEYRGNGNQVITMLQLGTVAPALVA"
     gene            complement(2739..3416)
                     /locus_tag="PKK81_09510"
     CDS             complement(2739..3416)
                     /locus_tag="PKK81_09510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV33157.1"
                     /translation="MRHVTGAVSAVVLLISSLSWAAAPEPANDEQKTLYALGAAISQS
                     LGPFTLNESELEFVKAGLVDGVLKHPQKVDLQVYGPKIQQMQQVRATALAEVEKKAGA
                     GFLAKAAAESGAKKTESGAIVTTIKEGKGATPKATDTVKVHYHGTLIDGTVFDSSVKR
                     GEPATFPLNQVIKCWTEAVQLIKVGGKSKLVCPSGIAYGDRGSPPVIKPGATLIFEVE
                     LLDIVKQ"
     assembly_gap    3529..3628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3747..4691)
                     /locus_tag="PKK81_09515"
     CDS             complement(3747..4691)
                     /locus_tag="PKK81_09515"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNV33158.1"
                     /translation="MRAQPQFPNEQTDTGEFKRQSDAFRHWVTEDGRSGYPAAAGRYH
                     LYVSLACPWAHRTIIVRALKGLTEVIGMTVVDPIRDDQGWAFHNGPGHSLDTTNGFHF
                     LSEAYRATDPNYHGRVTVPVLWDTVTKRIVTNSDDDLMRIFNGEFNRFTTSRLDLYPA
                     ALRPQIDEMNDFVYERVNDGVYRAGFATSQQVYERAARSVFVALDQLDTRLRDRRYLF
                     GAQFVESDWRLFVTLLRFDAVYHGHFKCNLRRIVDYPHLYGYLRDLYQVKGIAETVDF
                     DHIKRHYYVTHDDINPTRIVPIGPQLDLESPHGRDTLS"
     gene            complement(4790..5272)
                     /locus_tag="PKK81_09520"
     CDS             complement(4790..5272)
                     /locus_tag="PKK81_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33159.1"
                     /translation="MFQRLGFGLGLGWTHNLGPRRVDSVTVVNRIVRIDNERNDLVRF
                     MPEMHLWIDRWDEQRWSWGPFLTVAPGSRIIDAVGFGLMMGYRPHRQDQYSFNVGIGG
                     TLDLDARVLGDGLIANEPLPARETTARTKQTTAAGLLVLFSVGWDLAAPHHPAHTERQ
                     "
     gene            complement(7118..7612)
                     /locus_tag="PKK81_09525"
     CDS             complement(7118..7612)
                     /locus_tag="PKK81_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33160.1"
                     /translation="MVRLTTDRMKSPRHWVTFLTVGFCTVLFTPPLHAADSSGPWECS
                     SYTGDAHTRCLQAFIEIQRDKISKLEAEVHVQQNAVGQLKEQADRQSAMTADLQRQMA
                     EQSSSVSTYNYVAPGLLYGYPSVGFGLYLGRPWLYGSPFYGRPYAWGPRYYRPYYGRW
                     HRRW"
BASE COUNT         1405 a         2064 c         2604 g         1657 t
ORIGIN      
        1 cggttctttc cggccggtcg cgtaccctat cataatttac ggcgcgaccc tactcccggt
       61 ttcatccggc ggggatgacc aataagcagg gccgcagggc ggaggtcgcc tgagtggcgg
      121 tcggggtttc gtccaaggag gacatatgcg aggactaatg tggcgtgccg cgacgttggt
      181 gatgctcttg ctgggcacag ccggctgtgc cgattcactg agcggcctgt cggggtcggg
      241 ggtggcgaag gggaccgggc agaccgttac tgttcatccc cgtgaaatcg atgaggtgct
      301 ggacaaccct ggaatgggct ttgccgattt tcatttcggg ttcggacatc cgccggcacc
      361 ggaagagtat ccccatacga ccgtcgccta ttttcgttgg ccctgggcgg aactcgaacc
      421 gaaggaaggt cagtacaact tcgccctcgt ggatcgtgtg atcgagcagg ccaaggccaa
      481 gggagagaca ctcgccatcc gcatcgtgtc cgaatacaaa accggctcgc cccaatggtt
      541 gctcgacaag ggggtcggga gtgtgaagga gtccgacggc attttccccg actacaacca
      601 tcccgttttt ctcgactacc atgagcggct gatccgcgca ttcggtgaac gctacggccg
      661 ctccgtcgat atcgatcatg tcgacatcgg gtccatcgga tgttggggcg agtggaatac
      721 ggtgtgttgc gaaggtgtgg aagcccagtg taaggcattc tttccgaccg aggcgaatca
      781 gatcgccatc acggactggt acctgaaata tttcgcaggc actccgttgg tgatgctcca
      841 cggcggacaa ctgaagtatg cggcctcgca cggcgccggt tggcgcgggg attgtttcgg
      901 cgattacggc tatttcagtc ctgactggaa tcatatggaa cacgcctacc ctccggtact
      961 cgaagaggcg gttattgcga atgcctggaa gcgtgggccg gttcagatgg aggtctgcgg
     1021 gtacattcac gagtggtacg atcgcgggtt cgatctcgac cgtattctga atcagggctt
     1081 ggagtggcat ctctcggtgc tgaatgcgaa gtcgaagccg gtgcctgccg cctggcgacc
     1141 gcggttcaat gaatttttga aaaggatcgg gtatcgcttc gtcttgcgcg aactgacgca
     1201 ttcagcggag agtcaccccg gcgggccgct cgtccttcag tcgcgttggg agaataaggg
     1261 ggtggcgccg atctaccacg cctggccgct ggcctatcgg ctacgatcga gctcggatca
     1321 ggttgtggcg cagtggacca gtcctgccga tctcaagcaa tggctgccgg gaccttcgcc
     1381 ccgggtcgag gatacggtgg tggtgccgga gaccctgtcc gccggttcct atgcgttgga
     1441 tgtggcgatt ctctctgaag atgccctctc cgcccatgtc gagttggcaa tcgaggggaa
     1501 acgagcggac cggtggtatg cgctgtcacg cgtggagatt cgctgattgt tcggcgtggt
     1561 gtaggtctgt tcggccggtg tcggtgttat gagaggcgga tcgttgtggt gggaggcgtt
     1621 gttacgcgac cagtgccggc gcgacagtcc caagctgaag catcgtgatc acctggttgc
     1681 cgttcccgcg atactcgatc cgatcgaagc tgactttgtt ggccatggcg atcccgcggc
     1741 catgggtgtc gaaggctcgc tcggggtcga agttcaaata cttccgccac tcgaatccct
     1801 tgccctgatc ggtaatgtgc agggagagtt tgtctggatg gcggacaaag tgtgccgtgg
     1861 cctcgcggga cgagtagagg ggatcgctca gccgtcgctc gatttcattg tccagccgtt
     1921 cctcttcgat gaggcgtgat ttctctttat agccgatatt caagttgccg tgttcgatcg
     1981 cgttcaaggt gagttcgagg atgccggtga ccacccgatt ggcatcggga taggcatgcg
     2041 cgagtagcat cgccaggttt ttcgcctctt caggcgtttt gaaccggaac gttcccgagt
     2101 tcagcaggcg catggtcgtg gtggtccggc gcaggtcctg tcgtacgtcc aggtagctga
     2161 tgtgatcgcg gactgccgcg tcgacgatgg cttgcagggt cgctttgtcg aacggctttg
     2221 ccaggtaata gtaggctcct gcctggagtc cttccagggt ctgctgatgt gtggaggccg
     2281 ccgtctgcat gatcacggga atctggctca agacggtatg atgcttcatc ctgtggagga
     2341 cttccattcc gtccatctcg ggcatgatac ggtccaggag gacggcgtgg aatcggtccg
     2401 gttctcgttc cagcatgttc caagcttgga cgccgctttg tgccgtggtg cacgaatatt
     2461 ccttctcgcg taggaattcg agcaggatat tgaggttgag tggagaatca tccacgagca
     2521 ataactgtgt ttgcgggaac agcatgatat gtggcacctc cggttgccgg cttggccgtc
     2581 ggggtctagg gcagaagtcg tgggcctcgc atcggaatac aaggcactga tagccctatc
     2641 ggaagtcgac ggagaatact taagtgaggg ctgaaagaga gggaaaccga aggactccgc
     2701 cgggggataa gccccggtag agtccttccg gaagggggct actgcttgac gatgtccaac
     2761 aattccactt cgaaaatcag ggtggcaccc ggcttgatga ccgggggtga tccacggtcc
     2821 ccgtaggcga tgcccgacgg gcagaccagt ttgctcttgc cgccgacttt gatcagctgt
     2881 actgcttcgg tccagcattt gatgacctgg ttcagcggga aggtggccgg ttcgccgcgt
     2941 ttgaccgagc tgtcgaacac cgttccgtcg atcagcgtcc cgtggtagtg gactttcacc
     3001 gtatcggtgg ccttcggggt cgcgcccttg ccttccttga tcgtcgtgac gatcgcaccc
     3061 gattccgttt tcttcgcgcc cgactccgcc gcggcctttg ccaggaatcc tgctccggct
     3121 ttcttttcga cctcggccag cgccgtggcg cgtacctgct gcatctgttg aatcttcgga
     3181 ccgtacactt gcaaatcgac cttttgggga tgtttcaaga caccatcgac caaaccggct
     3241 ttcacgaatt ccagttcgga ttcgttcaaa gtaaacgggc cgagcgattg gctgatggcc
     3301 gcaccgaggg catagagcgt tttctgctcg tcgttggccg gctcaggggc ggccgcccag
     3361 ctgagcgagg atatgaggag caccactgcg gagacggcac cggtcacatg acgcatagac
     3421 gatcctttct gatggaaagc atgactctgc tcaccatatg ccagggagcc tgtgcggctc
     3481 aagataattc ttgggcaggc tgctgaaaac ggcctcccgc gtcgttctnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg agcagcctgc gaagattacg cagacttgtc
     3661 tgtagccttg ccgaatggaa ggcctgtctg gcgtccagga tgggtaagta tgtcgtctgt
     3721 ttcagcagtc ttgccgtgag gtgcggtcag gagagcgtat cacgtccgtg cgggctctcc
     3781 agatccagtt gcggtccgat ggggacgatg cgggtcgggt tgatgtcgtc atgggtcacg
     3841 tagtagtgcc gcttgatatg gtcgaaatcc acggtctccg cgataccctt cacctggtag
     3901 aggtcccgaa ggtagccata gagatggggg tagtcgacga tgcgccggag gttgcatttg
     3961 aaatgcccgt ggtagacggc atcgaaccgg agcagcgtga caaacaaccg ccaatcggac
     4021 tcgacgaatt gggcaccgaa gaggtagcgc cggtcgcgga gcctcgtgtc gagttgatcc
     4081 agcgcaacga agactgagcg cgcggcgcgt tcatacacct gttgggaggt cgcaaaaccg
     4141 gcgcggtaga ccccgtcgtt cacgcgctcg tatacaaagt cgttcatctc gtcgatctgc
     4201 ggacggagag ctgccgggta caggtccagc cggctggtgg tgaagcgatt gaactcaccg
     4261 ttgaagattc gcatgaggtc atcgtcggaa ttggtcacga ttcgttttgt gaccgtgtcc
     4321 cacaacaccg gaaccgtcac gcggccgtgg taattggggt cggtggcacg ataggcctcg
     4381 ctgaggaagt gaaacccatt ggtcgtatcc agggagtgac cggggccgtt gtgaaaggcc
     4441 catccctggt cgtcccggat cgggtcgacc acggtcatgc cgatcacctc ggtgagccct
     4501 ttgagcgcgc ggacgatgat ggtgcgatgc gcccaggggc aggcgaggga gacgtagaga
     4561 tgataccgcc cggctgcggc cggatatccg gagcggccgt cctcggtgac ccagtggcga
     4621 aacgcgtcgg actgccgttt gaattcgccc gtgtcggtct gttcattcgg aaactggggt
     4681 tgtgccctca ttgcacgatt ctccttggga gttctacctt agtcagaatc aagggggctc
     4741 gtcaattgtc gatagggtcg cgcgatgtcg tggtgcctcg aatgtaaggt cattgtcgtt
     4801 cggtgtgtgc tgggtggtgg ggcgctgcga ggtcccatcc gacggaaaag agtacgagga
     4861 gaccggcggc ggtggtctgc ttcgtgcggg ccgtggtttc ccgagccggc aggggctcgt
     4921 tcgcgatgag gccgtcgccc aggactcgag catcgagatc gagcgtgccg ccgataccga
     4981 cattgaaact gtattggtcc tgtcgatgcg gccggtatcc catcatcagg ccgaacccca
     5041 cggcgtcgat gatccgcgac cccggcgcga ccgtgagaaa cggcccccaa ctccaacgtt
     5101 gctcgtccca ccggtcgatc cagaggtgca tttccggcat gaagcgcacc agatcgtttc
     5161 gttcattgtc gatgcggacg atacggttga cgactgtgac ggaatccacc cgtctcgggc
     5221 caaggttatg tgtccatccg aggccgagcc cgaagccgag gcgctgaaac atccatggac
     5281 gttcagcgtc cgtgtccggc ggattctcct tcttgtccgt ggcgggagcc agggtcggca
     5341 gcggtgtgcg gtcagtcgag ggaccggtcg cgcctgtctg cggagcaaga gctgccgcag
     5401 aggaggagtc ggctgaagcg acaagcggag cggagtgagg gcgcgtcgaa tcagtggatg
     5461 gagtgagctg gggcggttgc cgcggtcttc gtctttcctc cgcagactcg atcactggtg
     5521 tccaggtacg acgctgttga tccgccgacg gtgaagccgc aggtttggtc tccacctggg
     5581 tatcgacggc ggcgaccagt ggtgtcggcg ttggtgatgg cgcagttgga ggttgtaccg
     5641 tgttctgcgg tgtgagcgac ttggtcggcg gcatgtcgga tacctgcggg gacaacggtg
     5701 tcaccgtgtc agcgggtggt tgggattcag tgagcagagg ttgcgaggcg attgccttac
     5761 tggcttcggg atctcttgtg ggaccggtcg cgatcggcgg cgccggtacc ggacctgtgt
     5821 gcggcctgga agtggcaaca gcgggcgaag tggccgccga agtcccctca ggtgcgggag
     5881 agagggattc cgcagcggtc ggaccggtgg aggattccgt cgccagagtg gatgggatgg
     5941 ggatgactgc gatatccggt tgcctgtcgt cctgcgccga catggctgtc ggcggattga
     6001 gcgacggtac ggccggaagc ggggccgcgc ttcgagacgg cgccggaggg gtactctggc
     6061 ccgcgcttga gggtgtcccc agatccggag ccgccatacc gggagctgcc tgtggacttt
     6121 ccgccgtcgc ggcgggcgat tggggcctcg tcggtatctc ggcggctggt atggcccgca
     6181 cgggtgttct gttaagtgcg tgccggagtg aaccattgct actttcggtt gccagagtga
     6241 tcggagttct tccgctcgca tctctgagct caggatcggc tccatagcga aggagtatgt
     6301 cgatcacgtt ggcttgccgg gtcagccggt ccgcccggga ttcaccggag agttcccaaa
     6361 atcgcctgac ggcctgatgg agcggcgtga atgcgagggc gttccgtgcc gaggtctgcg
     6421 cccctgtggc caggagtgcc tccacgcagg gtacctgccc ataaaaactc gcctgatgta
     6481 acgcggtatt gccggcttga tccgttgtct caacctccgc atgccggttg aggagtagag
     6541 tgaccagcgc cagattgccc ctgcctgccg ctgtgatcag cggcgtcgcg ccctgggcgt
     6601 ctcgtgcctc gatattcgcg ccctggtcaa gcagccgttt ggcttgggcc gcggttcctc
     6661 cggtgagggc cttgtggatc gagcgatctg aatggatcag cgaggcgttc ggtttgggtt
     6721 gcggcttatc gggacgcgcg tgactgacgg acgggagtcc gaaaatgaga agaagcagga
     6781 aggcgcaggt aacgccggtg ctgtggtgca agcgaaacac gggtccccaa caaggcaatt
     6841 caagaggttc tcggccggtg tatggtttag catacagtcc gcattggcgt cacctgagtg
     6901 tgcgaccggt gtggaacaat gtgcttagcg aagccgctgc tcggtcttgc atgggccgtt
     6961 ggatgggcaa tgaattgatg gtcgggtcag tcttcaggaa gaggtgggag cgacaaagaa
     7021 gggataacgc ggtgaggcct tgccaggggc ggaccctccg gttggaagag cactgagagg
     7081 atcacgtttc agcagctgtg gcgcctatgg tccgggatta ccaacgtcta tgccaacgtc
     7141 catagtaggg ccgatagtac cgtggtcccc aggcgtaggg ccggccgtag aacggtgagc
     7201 catagagcca tggacgtccg aggtacaggc caaaacccac agacggatat ccgtagagca
     7261 atccgggcgc aacataattg taggtcgata cggaagagga ctgctcggcc atctgacgtt
     7321 gaaggtcggc ggtcatggca ctttgccgat cagcctgctc tttcaactgg ccgacggcgt
     7381 tttgctggac atggacctct gcttccagct tcgaaatttt gtcgcgctgg atttcgataa
     7441 acgcctgcag gcatcgcgtg tgcgcatcac ccgtgtagct tgaacattcc caggggccgg
     7501 aagagtcggc ggcgtggagg ggaggcgtga agaggacggt gcagaaaccg actgtgagaa
     7561 acgtgaccca atgccgagga gatttcattc gatcagtcgt gagacgtacc atgaggttcc
     7621 tccctggcta gctggctata gcctaccatc cccgtgtcgg cgaagaccag gtctggggct
     7681 aaccttctga aacgaaccac gcgctgatcg gttgacaacc ggctgcaggg gaaggatgct
     7741 tagcggcggc aaccggttgt ggaaaggaga ggggaagaaa tgggacagaa gcaggtgatt
     7801 gcggtggtcg gggcgacggg gatgcagggg
//
LOCUS       DAOQRM010000116         7711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_9105_length_7711_cov_14.923589, whole genome shotgun sequence.
ACCESSION   DAOQRM010000116 DAOQRM010000000
VERSION     DAOQRM010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9105_length_7711_cov_14.923589"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..248
                     /locus_tag="PKK81_09530"
     CDS             123..248
                     /locus_tag="PKK81_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33161.1"
                     /translation="MPKIEGDLATALTLLHKYFEQKKIPFALIGALVPAILLHLR"
     gene            314..934
                     /locus_tag="PKK81_09535"
     CDS             314..934
                     /locus_tag="PKK81_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33162.1"
                     /translation="MIADLVTVGFVRSRSEQEHRLHYRTAEIDLIPYGIANGPDEVLV
                     WRKSGNRMNLAGFWDVFEHAVPVELTKSLTIPIIPLWLFSVLKIFAYLDRKFPRDLRD
                     LAYVLEHYESLEMGERRFEIVGAADNVTYENAGAYFLGTDIRTNLSSMAELNTVRDFL
                     DGVTDEYHPVINTVLREESLLKSGRRRIFVYQLIQACRLGLAGVAE"
     gene            1063..1233
                     /locus_tag="PKK81_09540"
     CDS             1063..1233
                     /locus_tag="PKK81_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33163.1"
                     /translation="MHTLMSAAEEFVNGVPYVQELDKERNALLEAIAQAQTLLSPEHV
                     AVTRPAKRKQKR"
     gene            complement(1239..2684)
                     /locus_tag="PKK81_09545"
     CDS             complement(1239..2684)
                     /locus_tag="PKK81_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV33164.1"
                     /translation="MNSLGRLIRRTALILMGGLLLGFSALIYVGGDTLLSRYVDGRLL
                     ELAETLGRIIEQRPDVIRGAGDELVVLGEKGRSQEEQHELREAAHSVRILSIDGQLLW
                     KGSDVVPRPPVSASLLEQVKQGQTVFDMVRLREGSPVRRVSIPVPRSGQVRYILQAEE
                     SLHFSKETLKGLAILLALGSGLVIVVAWAGSAWIARTVLTPIGLLSRRAETMSEADLG
                     ERLSLDSPFQEFHRLTHTFNAMMDRFQKSCESQRRFVDYAAHEMQTPLTVLQGNLEVT
                     LQKARSTDEYREALIGNLEQVEKLIALARSLLTLTKVTGDRPPLQLAPLELEPLLQEL
                     IIELKLLADDRRISLALDVVPVPTISADAQWLKQALINLLDNGLKYTPPGGSVTVRLR
                     RSDGSLEIAVRDTGPGIEAEHVPFLFDRFYRADKARARESGGTGLGLAIVKGIVEAHG
                     GTVSVDTQPGKGSEFTIRLPLDRPPESHSLN"
     gene            complement(2681..3364)
                     /locus_tag="PKK81_09550"
     CDS             complement(2681..3364)
                     /locus_tag="PKK81_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV33165.1"
                     /translation="MRILLVEDDADLAQFIRKGLKEERYAVDVASNGEQGFELGIANP
                     YDLIILDIMLPVLDGLSLCRRLRDTGVTAPVLLLTARDTVQDKVSGLDMGADDYLTKP
                     FAFAELLARARALLRRGGPQLQARLTAADLELDPATHRVCRSGNEIALTNKEYALLEF
                     LLRNKNRVLTRTAIIEHVWDISYDPMTNIVDAHIRSLRAKMDRDFSPALITTVRGAGY
                     MLEDEEAGR"
     gene            complement(3607..4272)
                     /locus_tag="PKK81_09555"
     CDS             complement(3607..4272)
                     /locus_tag="PKK81_09555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV33166.1"
                     /translation="MRIAHISPFLEKVSSESQDPQGRMIFHLTKQLTRLGHEVTVFAS
                     GDSRTAGELIPVAPLSMRSYPAPKRHLAEALAMLALEKAFATLPSFDIIHLHSGLSCL
                     PLMRRSPIPIVSTVYDALDAPEVIKVYREFKELALIATCAEQLKQVPDLNWQAVIPCL
                     DMLRWKNEPQAVEKIARAHETVYEWSTIGMGLPERVAPHLRVVTSAKANTTHERSIAA
                     SPY"
     gene            complement(4639..5673)
                     /locus_tag="PKK81_09560"
     CDS             complement(4639..5673)
                     /locus_tag="PKK81_09560"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNV33167.1"
                     /translation="MNTTKLMAACLVLSLVGVPTISFAKARGGSGGGFSSGRSSGSSG
                     SMGSRGSRTYQDNGAKPIEQSTTARQATSPPPSVASGPASTARPTPAAQPSWIQRNPL
                     LAGIAGGLAGSWIGHMLFGATDSSARTNEAGEQVGETGQAGASGPSGMLILLMLMGAG
                     ALFFYLRSRKPAVAPDFSGITRSSAVSGSLLDDSSVSTLRTATIDSEITSADKAAFQQ
                     SLTDIQTAWGKQDLSALRRLVTPEMLEYFSTGLAELTSQYQANRVEDVVLLEAEVRES
                     WVENTVQYATVSLHWSARDYTVSLSKAPGDAGYLIEGSAEKPIETTEVWTFMRYQHGK
                     WLLSAIQQVA"
     gene            5877..6026
                     /locus_tag="PKK81_09565"
     CDS             5877..6026
                     /locus_tag="PKK81_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33168.1"
                     /translation="MKQGYRLIVVDKDGVLVSEFQLTEQALAAPEAFVSALRESVEAI
                     EEAEP"
     gene            6293..7498
                     /locus_tag="PKK81_09570"
     CDS             6293..7498
                     /locus_tag="PKK81_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNV33169.1"
                     /translation="MANLLWGNVYYQDRFAGVLRQEPGERMSFTYDEHYLAGRHPSIA
                     HSLPLRTDTLVSQSGLPPFFDNLVAEGWLEEAQTRLLGKRRVSRFELLLAFGQDCAGA
                     VSVIDPDPEHRDAARPDDPMDLAVLSGRASLSGIQPKLALVRRDGQFRPARTDELSTH
                     IAKFPSPRHGDLTANEYLTTQALQAFLPGDEIVDLRMGHVAGFEEPTLIITRFDRTNG
                     GQRIHFEEFNQLLGNPSHAKYEGSHKSMADFIRQTPGCLPAEVYRLYLRILAGILLGN
                     TDMHLKNFAMFHTEAGLRLTPAYDAVSATLYGYNTIALSIGGSAHIPISEVKARTLIR
                     LGEEFGLSKPAMAMAAGHLEKRREAARNAIAESTTGTPELRDAIITFMEKRWNGTFAL
                     IGHALSKKP"
     gene            7447..7647
                     /locus_tag="PKK81_09575"
     CDS             7447..7647
                     /locus_tag="PKK81_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNV33170.1"
                     /translation="MERNLRLDWPRLVEEAVKRRKEQKLTQKTLAVLAGVSGPTVNAF
                     EQQRTSITLESALKIFRCLGMA"
BASE COUNT         1688 a         2340 c         2161 g         1522 t
ORIGIN      
        1 aagtcagcac cgcattcctg ccgcacatcc cattctgatt tacgcggagt tgttacacca
       61 cggcacggat cgtgaccgtg aaacggccca actcatgtac aaagaatttt tggaagactc
      121 cattgccaaa gattgaaggc gatctggcga cagcgctgac cctgctccac aagtatttcg
      181 aacaaaagaa gatccccttc gcgctcatcg gggcgcttgt gccggctatc ctgttgcatc
      241 tcagatagga gttcgagaaa ccagggatgc cgaccatgtg atccgagtgt cttcctgggc
      301 tgaatgggag actgtgatag ccgatcttgt caccgtaggg ttcgtacgaa gccgcagcga
      361 acaagaacac cgcctgcatt atcgaacggc tgagatcgac ctcatcccat atggcatcgc
      421 caatggcccg gacgaagtgt tggtatggcg caagtccggc aatcgcatga acctggcagg
      481 attttgggac gtatttgagc atgctgtacc ggtcgaatta acaaaaagtc ttactattcc
      541 gattatcccc ctctggcttt tctcggtcct gaaaatcttt gcctatcttg acaggaagtt
      601 tcccagagac ctccgtgacc tcgcatacgt gttggagcac tatgagtcgt tggaaatggg
      661 agagcgacga tttgagatcg tcggtgcagc ggacaatgtg acctacgaga atgccggtgc
      721 ttatttcctc ggaactgata ttcgcacgaa cctttcatcc atggctgagt taaacacggt
      781 gcgagacttt ctagacggcg tcaccgacga gtatcaccct gtcataaata ccgttcttcg
      841 agaggaatcc ttactaaaat caggcagacg aagaatattt gtctatcaac tcatacaagc
      901 ctgtcgttta ggactagcgg gcgtggccga gtgagacgac cggaattgga aaaagataac
      961 ccgacagccc cgcagttgta tgtcgggccc agaagaaggc gcgccctctc ccgtgcaacc
     1021 acaccatccc gccctgctgc acaggacgag attgctgcgg cgatgcatac cttgatgtcg
     1081 gcagcggaag aattcgtgaa cggcgtgccc tacgtgcaag agctggacaa ggaacggaac
     1141 gctctgctcg aggcgatcgc gcaggcgcag accctgctgt cgcccgagca cgtggctgtg
     1201 acgcgaccgg cgaagaggaa gcagaaacgg taggcgcgtt agttcaatga atggctttca
     1261 ggagggcggt caaggggcaa gcggatcgta aattccgacc cttttcccgg ctgcgtgtcg
     1321 acggagaccg tccctccgtg cgcctcgaca attcctttga caatcgccag ccctaaacct
     1381 gtccctccgg actcccgtgc cctggccttg tccgcccgat aaaaccgatc gaagaggaac
     1441 ggcacatgtt ccgcctcgat tccagggcct gtgtcccgga ccgcaatctc aagagatcca
     1501 tcggaacggc ggagacgaac cgtgaccgag ccgccgggcg gcgtatactt cagaccattg
     1561 tccagcagat tgatcaacgc ctgtttcaac cactgcgcgt ccgccgaaat cgtgggcacg
     1621 ggcaccacat cgagggcgag tgagatccgg cggtcatcgg caagcagctt cagttcgatg
     1681 atcagttcct gaagcagggg ctccagttcg agaggagcga gttgcaacgg cggccgatca
     1741 ccggtaacct tggtgagcgt cagcagagac ctcgccaacg caatcagttt ctccacctgc
     1801 tccagattcc cgatgagtgc ctcgcgatac tcgtccgtgg atctggcctt ttgaagcgtg
     1861 acttccaggt tcccctgcaa cacggtcagc ggcgtctgca tttcgtgggc ggcgtaatcg
     1921 acaaaccggc gctggctctc gcagctcttc tggaaccggt ccatcatggc gttaaaggtg
     1981 tgtgtcagcc ggtggaactc ttgaaacgga gaatcgagag ataatcgctc gcccaggtct
     2041 gcttccgaca tggtttccgc ccgccggctc aagagaccga tcggcgtcag gaccgttcgc
     2101 gcgatccatg cgctgccggc ccaggccacc acgatgacga ggcctgaccc gagcgccaac
     2161 aggatcgcga gccccttcag cgtttccttt gaaaagtgaa gcgactcctc cgcttgcagg
     2221 atgtaccgaa cctggccgct cctgggcaca gggatagaga cgcgacgcac aggggagccc
     2281 tcccgcaagc ggaccatatc gaagacggtc tggccctgct tgacctgttc gaggagggat
     2341 gccgacacgg gagggcgagg aaccacatcg gaccccttcc acagcagttg tccatcgatc
     2401 gacaagatcc ggacactatg cgcggcctcc cggagttcat gttgctcttc ctggctgcgg
     2461 cccttctccc ccaatacgac cagctcgtca ccggccccgc ggatcacatc cggacgctgt
     2521 tcgataatgc ggcccaatgt ttccgcaagt tcaagcagac gcccatcgac atagcggctc
     2581 aacagcgtgt cgccgccgac atagatcagc gccgaaaaac cgaggagcag ccctcccatc
     2641 aagatcagcg ccgtccggcg gatcaggcgt cccagtgaat tcatcgtccg gcttcctcat
     2701 cttccagcat atagccggcc ccccgcacag tcgtaatcaa cgccggagaa aagtcgcgat
     2761 ccatcttcgc ccgcaacgac cggatatgcg catccacgat attcgtcata gggtcataac
     2821 tgatgtccca cacgtgctcg atgatggcgg tcctggtcag cacacgattc ttgttccgca
     2881 agagaaattc gagcagcgcg tactccttgt tcgtcagcgc gatttcattg ccgctcctgc
     2941 acacccggtg agtcgccgga tccagctcca ggtcggcggc cgtcagccgg gcctgcagtt
     3001 gaggcccgcc ccgtcgcaac agggccctcg ctctcgcgag gagttctgcg aaggcaaacg
     3061 gtttcgtcaa atagtcatcg gcgcccatgt ccagaccgga gaccttgtcc tgcaccgtat
     3121 cgcgggcggt caacagcaag acaggcgccg tcacgccggt atcgcgtaaa cgacgacaga
     3181 gtgacaggcc atcgagcaca ggcagcatga tgtccaggat gatgagatcg tacggattcg
     3241 cgatgccgag ctcgaacccc tgctcgccat tagacgcgac atccaccgca tagcgctctt
     3301 ccttcaagcc ctttcggatg aattgcgcca gatcagcatc atcttctacg agaagaatgc
     3361 gcataccgat acatgctcca tcgaacgatc gaagggtgag gaaatcaata gcctgcgatg
     3421 ttcccgtggc cgggcctatc gtcatcacca tactaataca tcagcccgtc tcgattcgat
     3481 cagcgagaag gtagtgaaag aggagacact cggacaagca cgcacgcgca cctggatgct
     3541 gggaccagga actcagatgg tgcccgacga ccggaggaag ttcagccgct catcggctcg
     3601 tcgtcatcag tatggggaag ctgcgataga gcgctcgtgg gtggtgttgg ccttcgcgct
     3661 cgtgacaaca cgcaagtgag gcgcgacgcg ttcggggaga cccatcccga tcgtactcca
     3721 ttcgtagacc gtctcgtgcg cgcgcgcgat cttttccacg gcctgcggct cgttcttcca
     3781 ccgcagcatg tccaagcagg gaatgaccgc ttgccaattc aagtcaggca cctgtttgag
     3841 ttgctccgcg catgtcgcga tcaaggccaa ctccttaaat tcacgataga ccttgatcac
     3901 ttcgggggcg tccagagcat cgtacacggt cgagacgatc ggaatcgggc tacgacgcat
     3961 cagcggcaaa caagacaatc ctgagtgaag atggatgatg tcgaaggacg gcagcgtggc
     4021 gaacgctttc tccaatgcca gcatggccaa tgcctccgcg agatggcgtt tcggcgcagg
     4081 ataggatcgc atggaaagcg gagcgacggg gatcagctca ccggctgttc tgctgtcccc
     4141 actggcaaag accgtcactt catgtcccag ccgggtcaat tgcttcgtca aatggaagat
     4201 catcctgccc tgcgggtcct gactctcgga tgacaccttt tccagaaacg ggctgatgtg
     4261 agcaatccgc atggtctttt tccctttgtc cggtccagcg tgagacgtgc ccttcaggct
     4321 cgcgtccagc agcgcagacg agctgtcgac ggggcagcaa cggtcgccgt gacgaatgta
     4381 tggtttgagg gagcagtgca ggcagccgaa tcagccgccg ctctacgttt caaagagaag
     4441 gtacaacggc aatcatgaaa attcggcgaa gagggggtga agtcatcatg aagtgatctc
     4501 acgatgccgg agacgtggag gtcgtgcgat cgcacaagca agcgtactgc cgaaaacaaa
     4561 acggggacag ccactcgcgt gaccgccccc gtatccaacc agctcacact gtccccatcg
     4621 ggaccgctcg gcagggtctt acgcgacctg ctgaatcgcg gacaacaacc acttgccgtg
     4681 ttgatatcgc ataaaggtcc agacctcggt cgtctcgatc ggcttctctg cactcccctc
     4741 aatgagataa ccggcatcgc ccggtgcctt cgacagggac acggtatagt cccgggcact
     4801 ccaatgcagg ctcaccgtcg cgtactgcac cgtattttca acccacgact cccgaacttc
     4861 agcctcgagc agcaccacat cctccacacg gttcgcctga tactgactgg tcagttcggc
     4921 caatccggta ctgaaatact ccagcatctc cggcgtcacc agccgacgca atgcggacag
     4981 atcctgcttc ccccaggcgg tttggatgtc ggtcaacgat tgctgaaagg ccgccttgtc
     5041 ggctgaggtg atctcgctat cgattgtggc ggttcggagt gtcgacacgg acgaatcgtc
     5101 caagagcgag ccactcaccg cactgctccg cgtgatgcct gagaaatcag gcgccaccgc
     5161 aggcttacgc gatcgtagat agaaaaacag ggccccggcg cccatgagca tcaacaaaat
     5221 aagcattccg ctgggaccgg aagccccggc ctgaccggtt tccccgacct gttcacccgc
     5281 ttcgttcgtt cgcgcgctgc tgtcggtcgc cccgaacagc atatgaccga tccatgatcc
     5341 ggccagaccg ccggcgatac ccgccaacag aggattgcgc tgaatccatg acggctgcgc
     5401 agcaggcgtc ggcctggccg tcgaggccgg accgctggcg acggaaggcg gcgggctcgt
     5461 ggcctgcctg gcggtcgtag attgttcgat cggcttcgcg ccgttgtctt gataggtgcg
     5521 cgaaccacgg ctgcccatgc tgccggatga accactgctg cgcccactgg agaaccctcc
     5581 ccctgatccc ccccgcgcct tcgcaaagga aatcgtcggc acgccgacga gagacaacac
     5641 aagacatgca gccatcaatt tagttgtatt caagcgatac gtcctttcta ttgaacgtca
     5701 agatcaggct ggaaccagaa gttatcctaa cagcaatgcc ggaagaaaac ctacatttcc
     5761 tcctcgacgc cagacgcatc gcatcacaac cggcttcctt gtaagatagc ctctcacctt
     5821 cgttcggttc tgtcgtcccc ggaaacagac cgggcctccc gttgaaagga gtcatcgtga
     5881 aacagggtta tcgtttgatc gtggtggata aagacggtgt gttggtgtcg gagttccaac
     5941 tgaccgaaca ggcattggcc gcgccggagg cgttcgtgtc agcgctcaga gaatcggtcg
     6001 aggccatcga agaggcggag ccgtaagagc tgaagcagcg tgatcatctg acgatacaaa
     6061 cctcgatcat tctcccgctc ttatacatcc ctcccccatc cggcctgtgc cccgttgcta
     6121 ttccccactc gcgcggggaa aacctcgaac accagccgga tggtgcgtcc gcctcgctgg
     6181 accgggggcg atggtaaact ataatttcct cgatcattta cagcgcctaa tgcagttgac
     6241 tatgttttac cagattgatt cgagtgcaga ttctggtaaa ctttgactta ccatggcaaa
     6301 ccttctctgg ggcaatgtct attatcagga tcgcttcgcc ggcgtgctgc gccaagaacc
     6361 gggcgaacgg atgtccttta cctacgatga gcactatctg gccgggcgcc acccctccat
     6421 agcccactcg ctgcccttga ggacggacac tcttgtcagc cagtcggggc tgccgccatt
     6481 ttttgacaat ctggtggccg agggctggct cgaggaagcg cagacgcggc tgctgggaaa
     6541 gcgtcgggtc tcgcgcttcg aactgctgct ggccttcggc caggattgcg cgggcgctgt
     6601 ctccgtcatc gatcccgatc cggaacaccg ggacgctgcc cgcccggacg atccgatgga
     6661 tctcgccgtc ctgtcgggga gagcgtcgct ctccggcatt cagcccaaac tggctctggt
     6721 ccgacgggac ggacaattcc gtcccgcgcg aacggatgaa ctcagcaccc acatcgctaa
     6781 atttccctcg ccgcggcacg gcgatctgac ggccaacgaa tatctgacca cgcaggcgtt
     6841 gcaagcgttt ctgcccggtg acgagattgt cgatctgcgc atgggccacg ttgccggatt
     6901 cgaagagccg accttgatca tcacgcgctt cgaccggacc aacggcggac aacgcattca
     6961 tttcgaggaa ttcaatcagc tgctcgggaa tccttcccac gccaaatatg aaggcagcca
     7021 caaaagcatg gcggatttca tccggcagac gcccggttgc ctgccggccg aggtgtaccg
     7081 gctctacctg cgcattcttg ccggcattct ccttgggaat accgatatgc acctgaaaaa
     7141 tttcgccatg tttcacaccg aggcgggact gcgcctcaca cctgcctatg acgcagtatc
     7201 ggcgacgtta tacgggtaca acaccatcgc gttatcgata ggcggcagcg cccacattcc
     7261 catcagcgag gtcaaggcgc gaacgctcat ccgtttgggt gaagagttcg gattatcgaa
     7321 gccggccatg gccatggccg ccgggcatct agaaaagcgg cgggaagcgg ccaggaacgc
     7381 gattgctgaa agcaccaccg gcaccccgga attgcgggat gccatcatca ccttcatgga
     7441 aaaacgatgg aacggaacct tcgccttgat tggccacgcc ttgtcgaaga agccgtaaag
     7501 cggcgcaagg aacagaagtt gacgcaaaag acattggccg tcctcgcggg cgtcagcggg
     7561 ccgaccgtga atgcgttcga gcagcaacga acgagcatca cgctggaatc ggcattgaag
     7621 attttcagat gtctcggaat ggcctagccc gtaggtagca tcgtgggatc acgggtactc
     7681 gcgcggggaa catgcgaatg gtgttctttg a
//
LOCUS       DAOQRM010000117         7654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_9200_length_7654_cov_17.205553, whole genome shotgun sequence.
ACCESSION   DAOQRM010000117 DAOQRM010000000
VERSION     DAOQRM010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9200_length_7654_cov_17.205553"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1982)
                     /locus_tag="PKK81_09580"
     CDS             complement(138..1982)
                     /locus_tag="PKK81_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNV33171.1"
                     /translation="MAPNILLSCESISKSYGVRALFTDLSLALSDGDHVGLIGPNGSG
                     KSTLLKILVGLESPDEGTRTLRRHTRIGYVPQEPLFPPHHSIEQVLQDTLTEDGLDPH
                     EQGGRIARALSIGTFPDPEQAVSTLSGGWRKRLAITQALLLEPDVLLMDEPTNHLDLE
                     GIIWLEQLLKNRAKAFLVISHDRRFLESVTSRMVELNRCYPEGRFESRGCYSDFLEQR
                     DAALQAQADYQASLANRVRREVEWLRRGPKARTTKAKGRIQSAGKLIDELNQAESRSA
                     QSTIGIDFSASGRKSKQLVVAEQVSKSFNGTPVITNLDLLLGPGQRLGLLGPNGSGKS
                     TLLRLLAGTLEPDSGTITRADALRIVSFEQHRESLDQSTSLRRALAPSGDAVVYQDRS
                     IHLASWAKRFLFRPEQLDLPVSKLSGGEQARLLIARLMLQPADLLILDEPTNDLDIPT
                     LEVLEDSLLEFPGALVLVTHDRWLLDRVSTILLALDGTGRVDWFADYAQWESAQERAG
                     GSAPESKSDGRAAVQDETASTSPARKPKKMSYREQKEWGTIEESILKAEEEVTACQAA
                     MQDPAVLSDAAALQARSQALVAAQAEVERLYARWAELDEKRAQTVQSS"
     gene            complement(2132..2467)
                     /locus_tag="PKK81_09585"
     CDS             complement(2132..2467)
                     /locus_tag="PKK81_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33172.1"
                     /translation="MVTGPCSEEPLEEPGSFRERLSLLEAAIPYAATEAQPLLRHIYR
                     STRRLHDQLQRIDPTPPLPRSVVSEVRCFDCGTTKMESFHTSTQGGYHLCPSCFQQRL
                     RTGRARTAH"
     gene            complement(2621..4324)
                     /locus_tag="PKK81_09590"
     CDS             complement(2621..4324)
                     /locus_tag="PKK81_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNV33173.1"
                     /translation="MSTTPEPPTRSDFIREIVAADRAAGKHEGRVVTRFPPEPNGYLH
                     IGHAKSICLNFGIANENSGGICHLRMDDTNPTTEDPEYVQAIQDDVQWLGFDWQDKMF
                     FASDYFERLYDYAVGLIQKGLAYVDSLTADDMRKYRGTLTEPGQASPFRTRSVEENLD
                     LFRRMRAGEFPDGTHVLRAKIDMASPNINLRDPVLYRIRHVAHYRTGTAWCIYPAYDF
                     AHPLSDAMEGITHSICTLEFEDHRPLYDWVVEHCGTAQRPQQIEFARLNVTFTVMSKR
                     KLLDLVERKLVNGWDDPRLPTLKGLRRRGFTPEALRAFCEAIGVAKRDAVVEMQLLEH
                     FVREDLNKRAPRVMAVLRPLRLVIENYPEGQVEQLEAVNNPEDPSAGTRQVPFSRTLY
                     IDQEDFREDPPKQFFRLSPGREVRLRYAYIITCVGVVKDPATGEVTELRCTYDPETKS
                     GGAQAQRKVKATIHWVSAAHAIDAEIRLYESLMLMDPGKIPADQDWTQHLNPHSLERL
                     PTCKVEPSLAAIAPGTRVQFERVGYFCADPDSSPDKLVFNRTVTLKDTWAKIEKAQTP
                     R"
     gene            4523..5680
                     /locus_tag="PKK81_09595"
     CDS             4523..5680
                     /locus_tag="PKK81_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33174.1"
                     /translation="MMNGRWSACGLVVLLGILWAGGSQAVEPDSLEACRAQPFDAKMQ
                     CYQRHLETVLQAEGTEQTLTVLEQITVQDPEALREAHPLVHHVGQRSFARYGTAPLAL
                     AHCRDVFWSGCYHGVLQAFLSSLATVEPQHILPLCPISETVSAYSFQRYNCLHGLGHG
                     LTIQFRYDLLKSLAFCDALPGSWDRESCYGGVFMENIVTFQQAQRAQPAQTGSEHHHH
                     EATSFLNPQDLLYPCSVLNEKYLRACYLMQTSAVLTFLNYDFAQAFMQCARVEGEHQT
                     TCYRSLGRDISGYTLRDAVRVNELCRLGQADQIRQCFIGAVKDFILTDANPDPGLALC
                     RSLDGPFKKDCYATVGEMVIPLFDDKEKRAQACRKGEDEYVDACLAVATAF"
     gene            complement(5916..6329)
                     /locus_tag="PKK81_09600"
     CDS             complement(5916..6329)
                     /locus_tag="PKK81_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33175.1"
                     /translation="MTDVSPPSVQAFLVCDCVIEDSLTKKKSLIGLFSHLQSVTFPFQ
                     HHQLGLYFCITDAEGTYHVEIDLMFVNTDQLVCRASLPDIAIGDRLQISDFGINIPAL
                     LFPAPGRYEFRLRMNGRMIAQKDFHVVQVSAPPGT"
     gene            complement(6345..6869)
                     /locus_tag="PKK81_09605"
     CDS             complement(6345..6869)
                     /locus_tag="PKK81_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33176.1"
                     /translation="MELRKPDPAHSSHEAESDRHKHSLEIRLGSNIFRNTNGVIRVQG
                     KEQLVLELAPDQERILLTIDLYDGSGNHVAHLRRNRWAFNDGNRFSLNTSESPPTLFP
                     NLPWLKVTDQETGETVLEAAVAPGEKIHVATGKFYSHRGQLIEITSHFCRIGSTHTLF
                     GDVFEARGGTAVLG"
     gene            complement(6994..7428)
                     /locus_tag="PKK81_09610"
     CDS             complement(6994..7428)
                     /locus_tag="PKK81_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33177.1"
                     /translation="MVLRSTRLQTVLIAGLLPAFLSLAGCGASFLEGTIAPPYSSAWT
                     KCGATTKVRVKPPHSTVTVAYTEPTTGTDGKPLTNLAYTTIYYNAGDGPVIGKVVPAS
                     GKTGGAAVSQVVMIPLRDQSEQEVTVCVTATDSDEREGPASP"
BASE COUNT         1461 a         2096 c         2451 g         1646 t
ORIGIN      
        1 ctttggagct ggctacctag gttcgattcc tagtccccca gccaatttcc ctcccagacg
       61 atcctctgag aacgtacgcg actcaggtcg agatgtgagg ctgtcagcgc cacaactttg
      121 aacgggcggt cgttccttca cgaactctgc acggtctgtg cacgtttttc atccagttcg
      181 gcccaacggg catagagccg ctccacctca gcttgtgccg ccaccaatgc ctggcttcgt
      241 gcctggagcg ctgcggcatc cgacagcacg gccggatcct gcatcgcggc ttggcaggct
      301 gtgacctctt cctcggcttt gaggatactc tcttcgatcg tgccccattc cttctgttcg
      361 cgataactca tctttttggg tttgcgagcg ggacttgtcg aggcagtctc gtcctggacc
      421 gcagcacgcc cgtcactctt cgactccggg gcggatccac cggcccgctc ctgcgcactt
      481 tcccactggg catagtcggc aaaccagtcc acgcgtcccg tgccatccag tgccagcagg
      541 atggtcgaga cgcgatccag cagccaccga tcatgggtca ccagcacgag cgcaccgggg
      601 aattccagca ggctatcctc cagcacttcg agggttggaa tatccaagtc attggtcggc
      661 tcgtccagga taagcaggtc cgcaggctgg agcatgagtc gggcgatgag gagccgtgcc
      721 tgttcgccgc cggacagttt cgagaccggg agatccagtt gttcgggacg gaagagaaag
      781 cgtttcgccc atgaagccag gtgaatcgag cgatcctgat agacgacggc atcgcccgac
      841 ggggccagtg cgcgtcgcaa gctcgtcgat tggtccaggg actcgcgatg ttgctcaaag
      901 gagacgatac gcagcgcgtc ggctcgcgtg atggtgccgg aatcgggctc cagcgtcccg
      961 gccagtaacc gcagcagggt ggatttaccg ctgccgttcg gaccaaggag tcccagccgt
     1021 tggccgggcc cgaggagcag gtcgagattg gtgatgacgg gcgtcccgtt gaagctcttg
     1081 ctgacctgct ccgcgaccac gagttgtttg gactttcggc ccgaggcgga gaagtcgatg
     1141 ccgatggtcg actgtgccga acgggattcg gcctggttca attcgtcgat gagcttgccc
     1201 gctgattgga tccgaccttt ggctttggtc gtgcgggcct tggggccgcg ccgcagccac
     1261 tccacttcac ggcggacgcg attggctaat gaggcctggt agtcggcttg tgcttgaagg
     1321 gcggcatcgc gctgttcgag gaaatcacta tagcagccgc gggattcaaa ccggccttcg
     1381 ggataacagc ggttgagttc caccatgcga gatgtcaccg attcgaggaa ccggcgatcg
     1441 tggctgatca cgagaaaggc tttggcgcga ttcttcagca gttgctccag ccagataatg
     1501 ccttccaagt ccaaatggtt cgtcggctcg tccatgagta gcacatccgg ctcgagtagc
     1561 aaggcctggg tgatggccaa tcgtttgcgc catcctccgg agagtgtgga aacggcctgt
     1621 tccgggtctg gaaaggttcc gatgctgagg gccctggcga tccggccacc ctgttcgtga
     1681 ggatccagcc cgtcttccgt caaggtgtct tgcagcacct gttcaatgct gtgatgcgga
     1741 ggaaacaggg gttcctgcgg aacatagccg atgcgtgtgt ggcgccgcag ggtgcgtgtg
     1801 ccttcatccg gggattcgag ccctacaagg atcttaagca gggtcgattt gccggatccg
     1861 ttcggtccga ttaatcccac atgatcgccg tcggagagtg ccagggagag atctgtaaac
     1921 agggctctga ccccatagct tttgctgatc gattcgcaac tcaacagaat gttcggggcc
     1981 ataccctggg tacctctttc gtcacgttga aagcatgcag tatacggtgt ggtgggggct
     2041 cggggctagc ggctagcggc gtgggaggga gtggtctacc gtgccgccga gtgaaacgat
     2101 gtgaggtatc ttgcgggtgg gaatgtaaaa atcagtgcgc cgttcgagcg cgtcccgtgc
     2161 ggagacgttg ctgaaagcag ctcgggcaga gatggtaacc accctgtgtg gaggtatgaa
     2221 atgattccat tttggtggtc ccgcaatcaa aacagcgcac ctcgctcacc acagaccgtg
     2281 gcaagggggg cgtgggatca atgcgctgca gttggtcgtg cagacggcgt gtggagcggt
     2341 agatgtgccg gaggaggggt tgtgcttcgg ttgctgcata ggggatcgcg gcttcgagca
     2401 gggagaggcg ttcgcgaaac gagcccggtt cttcgagagg ctcttcactg caaggtcccg
     2461 tcaccatcgt tgaggctcct ttgtcgtgga gtgatgcccg atcagacgag tggcgattct
     2521 cgttcatcgg cgtgacaggg ttcaagggtg ccgaaggctg gagtccccgt actttgtgag
     2581 ggacggtgga atgggaggat tcgatgggac ttggtgctgg tcagcgaggg gtttgagctt
     2641 tctcgatctt ggcccaagtg tctttgagtg tgacggttcg attaaacacc aacttgtcgg
     2701 gtgacgaatc ggggtcggcg caaaagtatc ccacgcgttc aaattgcact ctggttccgg
     2761 gggcgatcgc agccaggctg ggctctacct tgcaagtcgg cagtcgctcc agcgaatggg
     2821 gattcaggtg ctgggtccag tcctggtcag cgggaatctt gcctggatcc atgagcatga
     2881 ggctctcgta tagccgaatt tctgcatcga tggcatgagc ggccgatacc caatggatcg
     2941 tcgccttcac cttccgttgc gcctgcgcgc cgccgctctt ggtttcaggg tcataggtgc
     3001 agcgcaactc cgtaacctca cccgtggctg ggtccttcac cacgccgaca caggtgatga
     3061 tgtaggcgta ccgcaggcgc acctcgcgtc ccggagaaag gcgaaagaac tgcttcggcg
     3121 gatcttctcg gaaatcttcc tgatcgatat agagggtgcg tgagaagggc acctgacgcg
     3181 tcccggctga agggtcttcc gggttgttga cggcttcgag ttgctcgacc tgtccctccg
     3241 ggtaattctc aatgaccagc cgcaagggcc gaagcaccgc catcacacgg ggcgcacgtt
     3301 tgtttaagtc ttcccggacg aagtgttcaa gcagttgcat ctcgaccacg gcatcccgtt
     3361 tcgcgacgcc gatagcctcg caaaaggccc gcagggcctc gggtgtgaaa ccgcggcggc
     3421 ggaggccttt cagtgtggga aggcgaggat catcccaacc gttcaccagc ttgcgttcca
     3481 ccagatcgag caattttcgt ttgctcatga cggtgaaggt gacgttcagt cgggcgaatt
     3541 cgatctgctg cggacgctgt gcagtgccgc agtgctcgac cacccagtcg tacagcggcc
     3601 ggtggtcctc aaattccagc gtgcagatcg aatgggtgat gccttccatc gcgtcggaga
     3661 gggggtgcgc aaagtcgtag gccgggtaga tacaccaggc tgtgccggtg cggtaatggg
     3721 ccacgtgtct gatccggtag agcacgggat cgcggagatt gatgttcggc gaagccatgt
     3781 cgatcttcgc ccgcagcacg tgggtcccgt cgggaaattc gccggcacgc atccgccgaa
     3841 acagatccag gttttcttcg acgctccggg tgcggaatgg actggcttgt cccggctcgg
     3901 tcagggttcc gcggtatttg cgcatgtcgt cggccgtgag gctgtcgacg tacgcgagac
     3961 ctttctgaat caggcccacg gcatagtcgt agagccgctc gaagtaatcg gatgcgaaaa
     4021 acatcttgtc ctgccagtcg aagccgagcc attggacatc gtcttgaatg gcctgcacat
     4081 attcaggatc ttccgtggtc gggttggtat catccatgcg gagatggcag atgccgccgg
     4141 aattttcgtt ggcgatgccg aagttgagac aaatcgattt ggcatggccg atatggagat
     4201 acccgttcgg ctccggcgga aagcgggtga ccactcggcc ttcatgtttg ccggcagcac
     4261 gatcggctgc gacgatctcg cggatgaagt ctgaacgtgt cggtggttct ggcgtggtgg
     4321 acataatggt tgcgagtccg tcgagttgca ggtgatgaaa cgcgcatggt tctatcacag
     4381 aacggcgggg tgggagaaga gggcccggcg tctcagggcg tggcgagtcg attttccctg
     4441 gatcgatcgg aaggactatt ttagaatcag gctgctgtgg aaggcggatg tctgacgggc
     4501 gccgagtaat ggagaataag agatgatgaa tggtcgatgg agcgcatgcg gactggtggt
     4561 gctgctcggc atcctctggg cgggcggaag ccaggctgtg gagccggact cactcgaggc
     4621 gtgccgggcc caaccgttcg acgccaagat gcagtgttat caacggcact tggaaacagt
     4681 ccttcaggca gagggcacag aacaaacgct cacggtcttg gagcagatca cggttcagga
     4741 tcctgaggca ttgcgtgagg cgcatccgct ggttcatcac gtcggccagc ggtcgtttgc
     4801 gcgctatggt acggcaccac tcgcgttggc ccattgtcga gacgtgtttt ggtctggctg
     4861 ctaccacggg gtcttgcagg ccttcctcag cagccttgcg actgtcgagc cacagcatat
     4921 tctgcccctg tgcccgatca gcgagacggt ctccgcctat tcgtttcagc ggtacaactg
     4981 tctccatggg ttgggccatg gcctgaccat tcaatttcgc tatgacctgc tcaagagcct
     5041 ggccttttgt gatgcgttgc cgggcagttg ggatcgggag tcctgctatg gcggcgtctt
     5101 catggaaaac atcgtgacgt tccagcaggc acaacgggcg cagccggcgc aaacgggcag
     5161 cgaacatcat caccatgagg ccacgagttt tctcaaccct caagacctcc tctatccctg
     5221 ctccgtcttg aatgagaaat acctgcgagc ctgttatctg atgcagacct ccgccgtgct
     5281 gacctttctg aattatgatt ttgcccaggc cttcatgcag tgcgccagag tggaggggga
     5341 gcatcagacg acctgttacc gcagtttggg ccgtgacatc agcggctaca cgttgcgtga
     5401 tgcggtgcgg gtcaacgaac tgtgccggct ggggcaggcc gatcaaatcc ggcaatgttt
     5461 catcggcgcc gtgaaagact tcattctcac cgacgccaat ccggacccag ggctcgcgct
     5521 gtgccgaagc ctggatgggc cgttcaaaaa agactgttat gccaccgtgg gtgaaatggt
     5581 gatccctctc ttcgacgaca aggagaagcg ggcgcaagcc tgccgcaaag gcgaagacga
     5641 gtatgttgat gcctgccttg cagtcgccac ggccttttag cgagggtctt tcggaactcc
     5701 cggtccagtc acgccgctcg gatcctgccc ctgatcagaa ccgtatctcg taaagcgcga
     5761 agcgcatctc ggtgaggata agcgcgtatg gctcatggct tatcgctggt agctacgcac
     5821 ctcactttcc agttcagacc acacgctctt ttcttcgacg agatacgaac gacgcttcac
     5881 ggtcgccgaa ggagtcgcgg ggaatccgtt cgaggtcagg tgccgggcgg ggcagacacc
     5941 tggactacat gaaaatcctt ttgcgcgatc atacggccgt tcatgcggag acgaaactca
     6001 taacgaccgg gcgcggggaa gagcagggcc gggatgttga taccgaagtc ggaaatctgc
     6061 agacgatctc caatggcgat atcggggagc gatgcgcggc agaccaactg gtctgtattg
     6121 acgaacatca gatcgatctc gacgtgatac gtgccttcgg cgtcggtgat gcagaagtac
     6181 aggccgagct gatggtgctg aaacgggaac gtcaccgact ggaggtggga gaacaggccg
     6241 atcagagatt ttttcttagt caggctatct tcgatgacgc agtcgcagac gaggaacgcc
     6301 tgcacgctgg gcggcgagac gtcagtcatg cgatccggcc attgtcagcc gagcaccgcg
     6361 gtgccgccgc gggcctcgaa gacatccccg aacagcgtat gggtggatcc gatgcgacag
     6421 aagtgcgacg tgatctcgat gagctggccg cggtgcgaat agaatttccc ggtcgccacg
     6481 tggatttttt cacctggtgc gacagcggct tccaggaccg tttcgccggt ttcctggtcc
     6541 gtgactttga gccatggcag attgggaaag agggtcggcg gggactcgct ggtgttgagt
     6601 gaaaagcgat tgccgtcgtt gaacgcccag cgattgcgac gtagatgggc cacatgattg
     6661 ccgctgccgt catagaggtc gatggtcagc aggattcgct cctgatccgg cgcaagctcc
     6721 agcacgagct gctctttgcc ttgaacccgg atgactccgt tggtattccg gaaaatgttg
     6781 gaaccgagcc ggatttccag gctgtgcttg tgccgatccg attcggcttc gtgggaggag
     6841 tgtgctgggt caggtttgcg tagttccatg cgtagagatc tagggcgacc cgaggacctg
     6901 ttgagcgacg cactcgacca tacgttcttg cctgagcaag tcgggagcaa ggtaggccct
     6961 tggcggggcg ctgtcaagtc cgttctggtc gggctaggga gaggcggggc cttcgcgctc
     7021 gtcggaatcg gtggcggtca cacagacggt gacttcctgt tccgattggt cacgcagcgg
     7081 aatcatgacg acttgggaca cagccgctcc gccggtcttt ccagacgcgg ggacgacctt
     7141 gccgatgacc gggccatccc cggcgttata gtaaatggtg gtataggcaa ggttggtcaa
     7201 gggtttgccg tcggtgccgg tcgtgggttc ggtgtaggcg accgtgacgg tcgaatgtgg
     7261 cggcttcacg cggaccttgg tggtggcacc gcatttggtc caagcgcttg aataaggggg
     7321 cgcgatggtt ccttccagaa agctcgcccc gcagccggcc agtgagagga aggccggaag
     7381 cagacccgcg atcaaaaccg tttggaggcg agtggagcgt agaaccatag tcctacttta
     7441 ggccaattca ataggctccg caagcagact gagcccaatt cggtctcgat tctctgttca
     7501 gcaagcctcg gcagcgtgta gtttgacttg ctgtttgaaa agccaaaacc cgctatggta
     7561 cagtcctgct ctttcaactg cctcgcagat tgcctctctg gtcccatcgt ctagcttggc
     7621 ctaggacgga gccctctcaa ggctcagaca cggg
//
LOCUS       DAOQRM010000118         7555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_9390_length_7555_cov_15.422667, whole genome shotgun sequence.
ACCESSION   DAOQRM010000118 DAOQRM010000000
VERSION     DAOQRM010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9390_length_7555_cov_15.422667"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /gene="coaE"
                     /locus_tag="PKK81_09615"
     CDS             complement(<1..465)
                     /gene="coaE"
                     /locus_tag="PKK81_09615"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247713.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNV33178.1"
                     /translation="MILVGLTGGVATGKSTVAKMFERCGALVIDADALAHQVVEPGKP
                     AWRAIIKTFGTAVLNPDRTLNRQSLSAIVFRNRSKLRRLEQIIHPRVAREQTRLTRQA
                     ARNDPKAIVIYDVPLLYEAGIDKRVDKVIVVTADRETQITRLNKRNGFTRAEA"
     gene            559..1590
                     /locus_tag="PKK81_09620"
     CDS             559..1590
                     /locus_tag="PKK81_09620"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HNV33179.1"
                     /translation="MKNCLFAVLFVCLSASAQVLSLPIAQAEEPASVERQPLVVALDG
                     SGQFLSIQEAVDAAKKGDTVLIRPGAYAEDVTIHSKENVRLIGAGMDQVTILGRERVG
                     VFHVGKWPYGATNIEISGITINEHGGHAMGMFNGRGIVLHHVRVKGMLFTQQVEDVRI
                     EDCIIGGSETTGVQFANSQAVMRSNFIHDNDHGVSVAGKSTVRLERNVITRSLFEAVI
                     VNDQAKATLLGNTFVKNGGGAAFLGTSQNEASGNIVSLNAYGFVVAPSSRVLFSYNAM
                     QNSGSNYLRSGTPNQPAPELKPDSDLTVDPRFVDTARDDFRLRADTALVKIGEFPYLG
                     ALPPLAGSH"
     gene            1748..1933
                     /locus_tag="PKK81_09625"
     CDS             1748..1933
                     /locus_tag="PKK81_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33180.1"
                     /translation="MASFRFDLEKRSTDGIPNLAELESGKLLGAILPMSEQAQLQLRD
                     SIEVIDYLMNQEGVVWS"
     gene            2256..4931
                     /locus_tag="PKK81_09630"
     CDS             2256..4931
                     /locus_tag="PKK81_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNV33181.1"
                     /translation="MSTSPGEEAVRPGSAEQQRLDEDDLRRKHWKRWGPYVSERAWGT
                     VREDYSSYGTAWESVPHDHARSKAYRWNEDGLAGICDRHQFVCLALALWNGRDPILKE
                     RMFGLTGNEGNHGEDPKEYYFYLDSTPTHSYMKFLYKYPQAAFPYAQLIEENRRRGRS
                     DPEFELLDTGVFAGDRYFDVGVEYAKASPEDLLMRIQIVNRGPDPAQLTVLPTIWFRN
                     TWSWGLDVRRPRMRKGESTSSVSIVEFDHEYYGPRRLLCEGGPDLLFTENETNTRRLY
                     GDVDGARHVKDGINDYVVQGDKGAVNPDQIGSKASAHYVVTALPDRPVTIRLRFTNGS
                     AEGALDPQAFDAVFAARLKEADEFYAALAPANVSEDARMVQRQAFAGLLWSKQFYHYD
                     VDRWLKGDPAGPEPPKARLHGRNADWTHLYNADVISMPDKWEYPWYAAWDLAFHCLPI
                     ALVDPRFAKDQLVLMLREWYMHPNGQIPAYEWALGDVNPPVHAWAAWRVYKIEKKRKG
                     AGDRIFLERVFHKLLLNFTWWVNRKDAEGKNIFQGGFLGLDNIGVFDRSKPLPTGGHI
                     EQSDATSWMGMYCLNMLSIALELAREDRAYEDVASKFFEHFVYICRAMNNIGDAKIEL
                     WNKADGFFYDVLHLPDGKKFPMKVRSMVGLIPLFAVETLDSELIDRLPRFKHRMQWFI
                     ENRPDFSLHIETQSQDGEVRRFLSLVNRHRLPKVLRYMLDEQEFLSPYGVRALSRYHK
                     DHPYVFSMMGTSQSVEYQPAESTNGLFGGNSNWRGPVWFPVNYLLIESLQKFHYYLGD
                     GFTVEYPTGSGQKRTLADVAAELSRRLTHTFLRGPDGRRPVYGGAEKFQQDPHWRDLI
                     LFYEYFHGDNGAGIGASHQTGWTGLVAKLIQQSGE"
     gene            4946..6931
                     /locus_tag="PKK81_09635"
     CDS             4946..6931
                     /locus_tag="PKK81_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNV33182.1"
                     /translation="MTIDSRRCQDLDRALSLEWLETNGRGGYASGTVAGANTRRYHAL
                     LLVARRPPVDRVVLVNHLEESVEVGGQSFPLSTNLYSGAVHPEGYRYCDGFSALPWPT
                     WRYASHGIRLERELLCPDGRDMVVVRWRLLGDAPSAVVLRVRPMLSGRDFHALHQENA
                     GVRSQATVHEGRVTWQPYDALPAVLAFHNGEYHHGPDWYRHIHYRIERERGLDHTEDW
                     WSPGECEFTLTPGSVAELLFTTEQLESVDVAQVVAAERRRRAECPVSAPTDDALAQEL
                     WVASGAYLVRRGNGQTVIAGYPWFADWGRDTFIALPGLCLVTGRYDVARQVIEAFASH
                     VSQGMVPNRFPDIGEQPEYNTIDASLWFIHAVDRYLHYSRDLDGVRRIAWPAIKQIVE
                     GYRRGTRFGIHMDDDGMITGGAEGVQLTWMDVKIGDWVVTPRHGKPVEIQALWVRALA
                     AAAELAAQFGEPAYAAQCRQDRARATESFRARFWYRTGGYLFDVVDGPIGDDASLRPN
                     QIYALALDDQLVTEAQAKHILQLVRERLLTPLGLRTLAPEDIRFCASYEGGVSERDGA
                     YHQGTVWPFLLGPFVTAWVNTYGDSPAVRRDARMFLDGLHEHLHEACLGHVSEIFDGQ
                     HPHRARGCVAQAWSVAEPLRALIEDLGAVHERSSSPR"
     gene            7074..>7555
                     /locus_tag="PKK81_09640"
     CDS             7074..>7555
                     /locus_tag="PKK81_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33183.1"
                     /translation="MRQHTSVTIVALLSAGLFLTGCQLFDSSTRQPAPRSASVMSMWE
                     LYRHCQSSGDVETVLSAAKQLQQSADTHVVPAPDVPKSLDRFVTRQPVRTTVDPKALA
                     ASCTLQAARTSLSAGREQEAEQLLYAVVLSYPESDYTFYVAQARVWIEELHRPGTLDA
                     V"
BASE COUNT         1421 a         2105 c         2419 g         1610 t
ORIGIN      
        1 ggcttcggcg cgggtgaatc cgttgcgttt gttcaggcgg gtgatctggg tttcacggtc
       61 ggcggtgacg acgatgacct tgtccacgcg cttgtcgatg ccggcttcgt agaggagcgg
      121 aacgtcgtaa ataacgatgg ccttgggatc gttgcgcgcg gcttgcctgg tcagtctggt
      181 ctgttcacgt gcgacgcggg ggtggatgat ctgctccagc cgacgcaact tggaccggtt
      241 ccggaagacg atggcgctca gggactggcg attgagggta cgatcgggat tgagaacggc
      301 cgtgccgaag gtcttgatga tggcgcgcca ggccggcttg cccggttcca ccacctggtg
      361 ggccagcgca tcggcgtcga tgacgagcgc cccgcatcgc tcgaacatct tcgctaccgt
      421 gctcttgccg gtcgcgacac cgccggtgag gcccacaagg atcatggggc gggaagatac
      481 catggggggc gagggcccgc aatgacgctc gattgacttc tcgccatgcc cggcggtatg
      541 accatttgta tgaagaacat gaaaaattgt ctattcgccg tcctctttgt gtgtctatcg
      601 gcctcggccc aggtcttgag ccttcccatc gctcaggctg aagagccggc gtccgttgaa
      661 cggcagccgc tggtggtggc gctggacggc tcgggtcagt tcctatccat tcaggaagcg
      721 gtggatgccg caaagaaggg tgacaccgtg ctcatcaggc ccggcgccta tgccgaggat
      781 gtgaccattc acagcaagga gaacgttcgc ctgatcggag caggaatgga tcaggtgacg
      841 atcctgggcc gtgagcgggt cggggtgttt catgtcggga agtggcccta tggcgcgacc
      901 aacatcgaga tctccggcat cacgatcaac gaacatggcg ggcacgcgat ggggatgttc
      961 aacggccgcg gcatcgtgct ccaccatgtg cgggtcaaag ggatgttgtt tacgcagcag
     1021 gtggaagatg tgcgcatcga ggactgtatt atcggcggca gtgaaaccac cggcgttcaa
     1081 ttcgcaaatt ctcaggcggt catgcggagc aatttcatcc atgacaatga tcatggtgtc
     1141 agcgtcgcag gcaaatccac ggtacgactc gaacgaaacg tcatcaccag gagcctgttc
     1201 gaggcggtca tcgtcaacga tcaagcgaag gcgaccttgc tcggcaatac ctttgtgaaa
     1261 aacggtggcg gggcggcgtt tttaggcacc tcgcagaatg aagcctccgg caatattgtg
     1321 agcctcaacg cctatgggtt cgtcgtggcc ccctccagcc gggtgctgtt ctcctacaat
     1381 gccatgcaaa actccgggtc caattatctt cggtccggaa cccccaacca accggctcca
     1441 gaactgaagc ctgattctga tttgacggtg gatccacgat tcgttgacac tgcccgggat
     1501 gatttcaggc ttcgtgcgga taccgcgctg gtgaaaatcg gtgagtttcc ctaccttggt
     1561 gccttgcccc ctctagccgg atcgcactga ctgccgtcag taaatcgata acaaattaag
     1621 aggttagccc tctgatccag tcgaaagagt ccttgtgcta tgcttcagtg tacgcatggc
     1681 atccaggttt ctccggtgtg gctcaagaat tgctgcacta cgaccgacaa gggattggag
     1741 gtaacaagtg gccagtttca gattcgacct ggaaaagcgg agtaccgacg ggattcccaa
     1801 cctggcggaa ctggaatccg gcaaactgct tggggcgata ctgccgatgt ccgagcaggc
     1861 gcaacttcag ttacgggaca gcatcgaagt catcgactac ctcatgaacc aagaaggcgt
     1921 tgtttggagc tagggcctgt gtcttcccgc tgagtgttcc ttcccagtcc cctgtcacca
     1981 cccatagttt ctttcgccgg acctcgcaaa gatcggtttg ctgactcgac tcctgtcgtg
     2041 cgcggtggac gctgacttgt ttcctatcct gacgccctcg aatctcggtt gaatcttttt
     2101 cgagcccggc gcatgctggc ccctgctgcg accttgctgg accgcctttc tcaacagcct
     2161 gtagcggatt ctgggaacgt cgacggctct gctggtttcc gcacttctgc tcggagtatg
     2221 tagtacgtca acacactggt aggagacctc gtgctatgtc gacatcgccg ggagaggagg
     2281 ctgttcgtcc ggggtccgct gagcagcagc gcctggatga ggacgatctg cgcaggaaac
     2341 actggaaacg ctgggggccc tatgtcagcg aacgggcttg gggaacggtc cgcgaagact
     2401 atagctcgta cgggacggcc tgggaatcgg ttccccacga tcatgcccgc tcgaaagcct
     2461 accgttggaa tgaagacgga ttggccggca tttgtgatcg gcaccagttc gtctgtctcg
     2521 cgctggcgct ctggaacgga cgggacccga ttctcaaaga acggatgttc ggcctcacgg
     2581 ggaacgaggg caatcacggg gaggatccca aagaatatta tttctatctc gactccacgc
     2641 ctacgcattc ctacatgaag tttctctata agtatccaca ggcggcgttt ccgtatgcac
     2701 aactcattga agaaaaccgt cgtcgcggcc ggtccgatcc ggaattcgag ctgctcgaca
     2761 cgggggtgtt cgccggcgat cgttacttcg atgtcggcgt cgaatatgcc aaggcctcgc
     2821 cggaggattt gctcatgcgg atccagatcg tcaatcgcgg tccggatccg gcgcagctga
     2881 cagtgctgcc gaccatctgg ttccgcaaca cctggtcgtg gggactcgat gtgcgccgcc
     2941 cgcggatgcg gaaaggcgaa tcgacgtcgt cggtcagtat tgtggagttc gaccatgagt
     3001 attacgggcc gcgacggttg ctctgcgagg gtgggccgga tctgttgttc accgaaaacg
     3061 aaaccaatac ccggcgtctc tacggcgatg tggatggcgc acggcatgtg aaagacggga
     3121 tcaatgacta cgtcgtgcag ggcgacaagg gcgcggtcaa tcccgaccag atcgggtcga
     3181 aggcgtccgc gcactatgtg gtgaccgcgt tgcctgatcg gcctgtgacg attcgtttgc
     3241 ggttcacgaa cgggtccgcc gaaggagcgc tcgatccgca ggcattcgac gcggtctttg
     3301 ccgcccgtct caaggaagcg gatgagtttt atgccgcact ggcccccgcg aacgtgtctg
     3361 aagatgcgcg aatggtgcaa cggcaagcct tcgccgggct cttgtggagc aagcagtttt
     3421 atcactacga tgtcgatcgc tggctgaagg gcgacccggc gggaccggaa cctcccaagg
     3481 cgcggctgca tggcaggaac gccgactgga cgcatctcta caatgccgat gtcatttcca
     3541 tgccggataa gtgggaatac ccctggtatg cagcctggga cttggcgttt cactgcctgc
     3601 cgatcgcgct tgtcgatccg cgttttgcca aggatcagct cgtcctcatg ttgcgcgaat
     3661 ggtacatgca tcccaacggg cagattcccg cctatgaatg ggccttgggc gacgtgaatc
     3721 cgccggtgca tgcctgggcg gcctggcggg tgtacaagat cgagaagaag cggaaaggcg
     3781 ccggcgaccg catctttctt gagcgggtgt tccacaagct cctgctgaat tttacctggt
     3841 gggtgaatcg gaaggatgcc gaggggaaga atatttttca gggcggattt ctggggttgg
     3901 acaacatcgg ggtgttcgat cgcagcaagc cgttgcccac cggcggacac atcgagcaat
     3961 ccgacgcaac cagctggatg gggatgtatt gcctgaatat gttgtcgatc gcgttggaac
     4021 tggcgcggga agaccgggcc tatgaagatg tcgccagcaa gttcttcgag cattttgtct
     4081 acatctgccg ggcgatgaac aacatcggcg atgcgaagat cgaactctgg aacaaggccg
     4141 acggcttctt ctacgacgtg ctgcacctgc cggacgggaa gaagtttccg atgaaggtgc
     4201 ggtcgatggt cggcttgatt ccgttgtttg cggtggagac gctggattcc gagctcatcg
     4261 accggttgcc ccgttttaag caccggatgc aatggttcat cgaaaaccgg ccggatttca
     4321 gtctgcacat tgaaacccag tcgcaggacg gggaggtgcg ccggttctta tcgctcgtca
     4381 accgtcaccg gctgcccaag gtcctgcggt acatgttgga tgagcaggag tttttgtcgc
     4441 cgtatggcgt gcgggctctg tcccgttatc acaaggacca tccctacgtg ttctcgatga
     4501 tgggtacgag ccaaagcgtc gaatatcaac cggcggaatc caccaacggt ctcttcgggg
     4561 ggaattcgaa ttggcggggg ccggtctggt ttcccgtgaa ctacctcctg atcgaatctc
     4621 ttcagaagtt tcactattat ttgggcgacg gcttcacggt cgagtacccg acggggtcgg
     4681 gacagaagcg cactctcgcc gacgttgcgg ctgagctgtc ccgacggctc acgcatacct
     4741 ttctgcgcgg gcccgatggt cggcgtcccg tctacggcgg tgcggaaaag tttcagcagg
     4801 atccgcactg gcgcgatctc atcttgttct atgaatattt ccatggcgac aacggcgccg
     4861 ggatcggcgc gagccatcaa acaggctgga ccgggctggt ggccaagttg attcagcagt
     4921 cgggcgagta agccggaggc acgggatgac catcgattcg agacgctgtc aggaccttga
     4981 ccgggcactc agtctggaat ggctggagac gaacgggcgg ggcgggtatg cttcgggcac
     5041 cgtcgccggc gcgaatacgc gacgctacca tgcgttgctg ctggtggcca gacgcccgcc
     5101 ggtcgatcgt gtggtcctgg tgaatcatct cgaagaatcg gtggaagtcg gcggccaatc
     5161 ctttccgctc tcgacaaatc tgtatagcgg cgcggttcat ccggaagggt atcggtattg
     5221 cgatgggttc tccgccctgc catggccgac ctggcgttac gcctcccatg gaatccgctt
     5281 ggagcgtgaa ctgctctgtc cggatggccg cgatatggtc gtcgtgcgat ggcggttgct
     5341 tggcgacgca ccgtctgctg tggtgctgcg ggtcagaccg atgctctcgg gtcgcgactt
     5401 ccatgcgctg catcaggaga acgccggggt gcgcagtcag gccacggttc acgagggccg
     5461 tgtcacgtgg caaccctacg atgcgctacc tgccgtgctg gcgttccaca acggggagta
     5521 ccaccatggg ccggattggt accgccacat tcactatcgg atcgagcgcg aacgcggcct
     5581 ggaccatacg gaggactggt ggtcgccggg cgaatgtgag ttcacgctca ctccgggatc
     5641 ggtggcggaa cttctgttca cgaccgaaca gcttgagtcc gtcgatgtcg cgcaggttgt
     5701 agcggcggag cggcggcggc gcgcagagtg tccggtatcg gcgccgaccg acgatgcgtt
     5761 ggcgcaggaa ctctgggttg cgagcggcgc gtatcttgtc cggcggggaa acgggcagac
     5821 ggtgatcgcc gggtatccct ggtttgccga ttgggggcga gacacattta tcgccttgcc
     5881 cgggctctgc ctggtgacgg gtcgttacga cgtggcccgg caggtgatcg aggcctttgc
     5941 gtctcatgtg tcgcaaggga tggtgccgaa tcgatttccc gacatcggcg aacagcctga
     6001 gtacaacacg atcgatgcgt cgttatggtt tattcatgcc gtggaccgct atcttcacta
     6061 tagccgcgac ctcgacggag tacgccggat cgcctggccc gcgatcaaac agattgtgga
     6121 ggggtatcgt cgaggcacgc ggttcggcat tcacatggat gatgacggga tgattaccgg
     6181 cggcgcggag ggcgttcaac tcacctggat ggatgtcaaa atcggcgatt gggtggtgac
     6241 tccccggcac gggaaaccgg tcgagattca agcgctctgg gtgcgggcat tggcggcggc
     6301 tgccgaactg gcggcgcaat tcggggaacc ggcgtacgcg gcacaatgcc ggcaggatcg
     6361 tgccagggcc accgagtcgt tccgggcccg cttctggtac cggaccgggg gctatctctt
     6421 cgatgtggtg gacgggccga taggagacga tgcctcgctc cgtccgaacc agatttatgc
     6481 cttggcgttg gatgatcagc tggtgacgga ggcgcaggct aaacacatct tgcagctcgt
     6541 gagagagcga ttgctcacgc cgctgggctt acgcacgttg gcaccggagg acattcgatt
     6601 ctgtgcttcg tacgagggcg gggtctccga gcgggatggc gcgtaccatc aaggcaccgt
     6661 atggccgttt ctgctcggcc cgttcgtgac agcgtgggtg aacacctatg gcgatagtcc
     6721 ggcggtgcgg cgggatgcgc gcatgtttct cgacgggttg cacgaacatt tacacgaagc
     6781 ctgcctgggc cacgtgtcgg agatcttcga cggtcaacac ccgcatcgag cgcgtggctg
     6841 cgtggcgcag gcctggtccg tggccgaacc cttgcgcgcg ctcattgaag atctcggcgc
     6901 ggttcatgag cgatcatcga gcccgcgctg aattgtgacg ttgcggttcc ttcatcaatt
     6961 gccggttcca tgctgacatg agcgcgtgag tgctcctgtc ttgtcacgtt ccttccagat
     7021 tctgctccgg aaatcttgac agccaaagaa gcgatccggt aaggataagg aagatgcgtc
     7081 agcacacatc cgtcacaatt gtcgcactcc tgtccgccgg gttattcctg accggctgtc
     7141 agctcttcga ttcctcgacg cgtcagccgg cacctcgctc ggcttccgtg atgtcgatgt
     7201 gggaattgta ccggcattgc caatcgagcg gcgatgtcga gacggtcttg tcggcagcga
     7261 aacagctgca gcagtccgct gatacgcatg tggttcccgc tccggatgtt cccaagagtc
     7321 ttgaccggtt tgtcacgcga cagccggttc gcacgactgt tgatcccaaa gcgctggccg
     7381 cttcgtgcac cttgcaggcg gcccggacga gcctgagcgc cggacgtgag caggaagccg
     7441 agcagctgct ctacgccgtg gtgctgagtt atccggagag cgactatacc ttttacgtcg
     7501 cgcaagcaag ggtctggatc gaggagttgc atcgtcccgg cacgctggat gctgt
//
LOCUS       DAOQRM010000119         7549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_9402_length_7549_cov_13.796104, whole genome shotgun sequence.
ACCESSION   DAOQRM010000119 DAOQRM010000000
VERSION     DAOQRM010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9402_length_7549_cov_13.796104"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKK81_09645"
     CDS             complement(<1..414)
                     /locus_tag="PKK81_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33184.1"
                     /translation="MKHAQVGVHPLLSVTVMAGVLGLAGYVQAAAPGETTGLTGFTQA
                     TDQSAALSVESAAPVPERAVAAGSAATMLTKVEAKPDGSRMALILSGNGVFTHTVKMI
                     GDRRMVVDMPHIQSDGHQSQLMVGHALLSRVRFGYH"
     gene            complement(411..1040)
                     /locus_tag="PKK81_09650"
     CDS             complement(411..1040)
                     /locus_tag="PKK81_09650"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNV33185.1"
                     /translation="MPNARGRTVKRRIWWRGSMAVAAVLLVSGGAVSPVEAKTLPHLR
                     QVASMRPADSLKVMPLPEGKRSEPAVEAPASRSEAAVSASGDSLSTEFSGTSYDPSGR
                     RDPFLPMIQLGQQTEQDVTLPPLQRVGLTELSLIGVLWGNYGYTAMVQTPDGKGYSIR
                     RGTRIGPNNGVVSSITERGIIVQERFTDVYGNKQEREYVKLLHPKEGTE"
     gene            complement(1058..1681)
                     /gene="pilO"
                     /locus_tag="PKK81_09655"
     CDS             complement(1058..1681)
                     /gene="pilO"
                     /locus_tag="PKK81_09655"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNV33186.1"
                     /translation="MSLNAISLDGLRSIPTGQKVALLGLLVAAILVGFYFYVVDPKTM
                     ELEAVQGQVAQLDTEIQNLTLKVKHLDELVAASKQLEIELAAKKERLPPEEEAVMLLK
                     QVSDLGMRLGLDVRLWKPGTQAEDPSKLFIRMPINVEVAGGYHTAAIFFDRISKLSRI
                     VTVQDVRIGAARVDQGRVVTQTVFDLVAYAAPGEKKAAAPVPAAKPK"
     gene            complement(1683..2237)
                     /locus_tag="PKK81_09660"
     CDS             complement(1683..2237)
                     /locus_tag="PKK81_09660"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNV33187.1"
                     /translation="MIRINLLATGPRSRKAKPQWDVRAEALIGIGVLLVTLTGCWYYA
                     ASLDEDIQAKQSEKQVKDKQVAQLKEQVKAVQDFEEKKKQLEAKNRIIDQLEKSRTGP
                     VKVLDHVSQSLNPLKVWLVRLNLKGNNVELEGRALTNDDVVEFVNNLRRTDQFGAIKL
                     MESRAGLDNKMNTYQFKLDLSMKG"
     gene            complement(2234..3331)
                     /gene="pilM"
                     /locus_tag="PKK81_09665"
     CDS             complement(2234..3331)
                     /gene="pilM"
                     /locus_tag="PKK81_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNV33188.1"
                     /translation="MLASLKNLADMEFLSMFSPQRQLLGLDIGSSSIKLVQIKEHRGR
                     YTLQKFAVKELEPEVIVDGTVMDEGRVVSAIKELLAEQNVKLKQVAISISGHAVIVKK
                     ISLPPMPDEELDAQVKLSAEQYIPFDINEVNLDFHVLPPSENPDEQSEMSIVLVAAKK
                     DKINELTELVKAAGLSPVVMDVDAFAVENMYGVTSPATQDDTTILVNIGASVMNVNIV
                     GGGVSLFTRDIPLGGNRYSEAVQREMGVSFEEAEQLKKSDRDDDHTLASVMESVNAEV
                     ASEIARTIDYFKTTSSESEIARVLLFGGGAKVKGLAQQLRDRMHVEVEIANPFNEIDT
                     SQCDVDPDQLTEMGPLAAVGVGLALRTVGDR"
     gene            complement(3345..3560)
                     /locus_tag="PKK81_09670"
     CDS             complement(3345..3560)
                     /locus_tag="PKK81_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNV33189.1"
                     /translation="MKKVSDVPKKKLYKTNEVCEMFDISRATLFRWEREGLITGPPRD
                     WRNWRLYTAENVEQIRHVMGGGRKEVA"
     gene            complement(3696..4577)
                     /gene="rapZ"
                     /locus_tag="PKK81_09675"
     CDS             complement(3696..4577)
                     /gene="rapZ"
                     /locus_tag="PKK81_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNV33190.1"
                     /translation="MLALLMAALNLVVISGLSGSGKSHALKAFEDAGYFCVDNLPPAL
                     LPTFVELCHQQGGEIKNVALGIDIRERVFFGDLAKVLGELKAQGHALQLVFLEAREEV
                     LVRRFSESRRPHPLLPHMPVVEGVRFERERLSELRKHADRVIDTSNITVHELRELLIR
                     QFRQETAARRMTISLVTFGYKFGVPYDIDLLFDVRFIRNPFFVPELKALTGEDARVQQ
                     YVLGDPAAGQFLEHLQGLFGFLVPLYERDQRSYLSIGIGCTGGRHRSVTMAVHLREQF
                     AALGYDVSVTHRDMQKP"
     gene            complement(4549..5100)
                     /gene="raiA"
                     /locus_tag="PKK81_09680"
     CDS             complement(4549..5100)
                     /gene="raiA"
                     /locus_tag="PKK81_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNV33191.1"
                     /translation="MRLMITGRHVAVTPALREYIETRMERLDRYGLKLGTLQILLSVE
                     KFHHVAEVVGVVQSRRLQAKTSTEEMYASIDEVVDKLGAQLRKLKERKVNHKFGDQPR
                     VRAVARKAPRAARNEVEVVRPTLEVLTVEEAVEALNSAKLAVLVFVNALSGTVQVLQR
                     LPNGRVSLIDPASDRARTAHGRA"
     gene            complement(5207..>6147)
                     /gene="rpoN"
                     /locus_tag="PKK81_09685"
     CDS             complement(5207..>6147)
                     /gene="rpoN"
                     /locus_tag="PKK81_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNV33192.1"
                     /translation="KDVQGFDPNGVAARDLSECLLLQLKFLGRSQIGSLGSRPGVLKG
                     TVLESIVLHHLKDLEKKQYNRIAKALNISMDDVFEATRIIEGLEPKPGRPFSNTQNYA
                     IVPDVFVVKNEGVWEVLLNDDGLPRMRISPYYKQLMSSGQSGSAETKAYLDDKLRAAQ
                     WVIRSIEQRNKTIVKVVSSIVKFQEGFFERGIQHLKPLVLKQVAEDIGMHESTISRVT
                     ANKYMYCPQGMLELKFFFNAGLQRADQPTDMLSSLTVREMIRQMVAAEDARKPLKDEE
                     IAARLRTQQVLIARRTVAKYRAEDNIPSATQRRKFF"
     assembly_gap    6148..6247
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6248..6528)
                     /locus_tag="PKK81_09690"
     CDS             complement(<6248..6528)
                     /locus_tag="PKK81_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33193.1"
                     /translation="MKLRLDLRLSQKLIMTPQLQQAIKLLQLSRLELQQSLQQHLMEN
                     PLLDELATETEESEETATAEREQPDTSTTVSSEVRGEGDDKPAESGENA"
     gene            complement(6549..7274)
                     /gene="lptB"
                     /locus_tag="PKK81_09695"
     CDS             complement(6549..7274)
                     /gene="lptB"
                     /locus_tag="PKK81_09695"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNV33194.1"
                     /translation="MPDRLAANGLVKSFRGRKVVKGVSLDVQAGEVVGLLGPNGAGKT
                     TIFDMMVGLCQPDEGTIALSGQEITDLPMYKRARRGIGYLPQESSVFRRLSVEHNILA
                     ILEMLGYSRSERMERVETLLKELDLVHIRTSKAYALSGGERRRLEITRALATSPSFML
                     LDEPFAGIDPIAVADIQQIIIRLKQRNIGVLITDHNVRETLSITDRAYIINEGTILEA
                     GPPGVIEKSEMARAVYLGEGFRL"
     gene            complement(7331..>7549)
                     /locus_tag="PKK81_09700"
     CDS             complement(7331..>7549)
                     /locus_tag="PKK81_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNV33195.1"
                     /translation="RVRIEKEDGRATCQKAVYYEDEKKIILTGDPVAWQKGTRVSGKR
                     IIMFLDEDRSVVEGDSQVVIEGESGGKR"
BASE COUNT         1518 a         2392 c         1871 g         1668 t
ORIGIN      
        1 gtgatacccg aatcggactc tcgacaacaa ggcatgaccg accatcagct gtgactggtg
       61 gccgtccgac tgaatatgcg gcatatcgac gaccatccga cgatccccga tcatcttcac
      121 ggtatgcgtg aagacgccgt tcccgctcag aatcaacgcc atccgactcc catcaggctt
      181 cgcttccact tttgtcagca tcgtcgcagc cgagccggcg gcaactgcac gctcagggac
      241 gggtgcggcg ctctccacgg acagggccgc actctggtca gtggcctgcg tgaatccggt
      301 cagtccggtc gtctcgcctg gcgccgcggc ttgcacatat ccggcaagcc ccaacactcc
      361 ggccatcaca gtgacactca gcagcggatg tacaccaacc tgtgcatgtt tcattctgta
      421 ccctctttcg ggtgcaggag cttgacgtat tcccgctcct gtttattccc gtacacgtcc
      481 gtgaaccgct cttgaacgat gatgcctcgt tcggtgattg aactgaccac accattgttg
      541 ggcccgattc gtgtcccgcg gcggatgctg taccctttgc cgtccggcgt ctgaaccatg
      601 gccgtgtaac cgtaattgcc ccagagcacc ccgatcaaac tcagttccgt gaggccgacg
      661 cgctgcagcg ggggaagagt cacatcctgc tctgtctgct ggcccagctg gatcataggc
      721 aggaagggat cccgacgacc cgacggatcg tacgaggttc ctgaaaattc cgtcgacaat
      781 gaatcccccg atgctgagac cgccgcttcg gaacgcgagg ccggagcttc aacagccggc
      841 tcagaccgct tcccctcggg caacggcatg accttcaagg aatcggccgg ccgcatggac
      901 gccacttggc gcaagtgagg cagtgttttc gcctcaacag gcgacaccgc gcctccactc
      961 accagcagga ccgctgcaac ggccatcgac ccgcgccacc agattcgtct cttcacggtg
     1021 cgtcccctcg cgttgggcat aagctaggct ccaatcctca ctttggcttc gccgcaggaa
     1081 cgggcgcggc ggcctttttc tctcccgggg cagcataggc caccagatcg aacaccgtct
     1141 gcgtgaccac acgtccttga tcgacacgag ccgcgccgat ccgcacatcc tgaaccgtca
     1201 cgatacggga cagcttgctg atacgatcga agaagatggc ggctgtatgg tatccgccgg
     1261 ccacttccac gttgattggc atccgaatga agagcttcga cggatcctca gcttgcgtcc
     1321 ccggcttcca gagacggaca tccaacccca gtcgcatgcc aagatcggac acttgcttga
     1381 gcagcatcac cgcctcttct tccggcggga ggcgttcctt cttcgcggcc aattcgattt
     1441 ccagctgctt gctcgccgcc accaattcat ccagatgttt gaccttcaag gtcagattct
     1501 gaatctcggt atccaactgg gcgacctgac cctgaaccgc ttccagctcc atggtcttgg
     1561 gatcgaccac gtagaaatag aaccctacca aaatcgccgc caccaacagt ccgagcagcg
     1621 cgactttctg acctgtcgga atgctgcgga ggccgtctaa gctgattgca ttcagactca
     1681 tgttagcctt tcatcgacag gtcgagtttg aattgatagg tgttcatctt gttgtccagt
     1741 cccgcccggc tctccatcaa cttgatggcg ccgaattgat cggtccgccg caggttgttc
     1801 acgaattcca ccacatcatc gttcgtcagc gcgcgacctt cgagctcgac gttgttgccc
     1861 ttgagattca gccgcacgag ccagaccttc aggggattca gactctgact gacgtggtcc
     1921 aacaccttga ccggtcccgt ccggctcttt tcgagctggt cgatgatgcg attcttcgct
     1981 tcaagctgct tctttttctc ttcaaagtcc tgcacggcct tgacttgctc tttcagctga
     2041 gcaacctgct tgtccttgac ctgcttctcg ctctgcttgg cctggatgtc ctcatcaagg
     2101 gaagcggcgt aataccaaca ccccgtcaag gtgaccagca acaccccgat gccgatcaag
     2161 gcctcagcac gtacatccca ctgcggtttg gctttcctgg accggggtcc agttgcgagc
     2221 aagttgattc taatcatcgg tcccccaccg tccggagcgc gaggcctacc cccaccgccg
     2281 caagcggccc catttcggtc aactgatcgg gatcgacgtc gcactgcgaa gtgtcgatct
     2341 cattaaaggg attcgcgatt tccacctcga cgtgcatgcg atcgcgaagc tgttgagcca
     2401 ggcccttgac cttagctccc ccgccgaaca gcaagacccg cgcaatttcg ctctccgaag
     2461 aggtcgtctt gaagtaatcg atcgtgcggg cgatctccga cgccacttcc gcattcacac
     2521 tctccatgac cgatgctagt gtatgatcgt cgtctcggtc gcttttcttg agttgctccg
     2581 cctcttcgaa cgagacgccc atctcccgct gcacggcctc ggaataccgg ttccctccca
     2641 agggaatatc gcgagtaaag agggagactc cccctcccac gatattcacg ttcatcacgc
     2701 tggccccgat gttcaccaga atcgtggtgt cgtcttgcgt ggcgggcgag gtcacgccat
     2761 acatgttctc caccgcaaat gcgtcgacat ccatcacgac cggactcagt ccggccgcct
     2821 tcaccagttc ggtcaattcg ttgatcttgt cctttttcgc cgcgaccagc acaatcgaca
     2881 tttcgctctg ctcgtccgga ttctccgacg ggggtaagac atgaaagtcc aggttcactt
     2941 cattgatgtc gaagggaatg tactgttcgg cagacagttt cacctgtgca tcgagctcct
     3001 catcgggcat cggcggcagg gaaattttct tcacaatgac cgcatggccg gagatggaga
     3061 tggcgacctg cttcaatttg acattctgtt cggccaacaa ttccttgatg gccgacacga
     3121 cacgcccttc gtccatgacc gtgccgtcca cgatcacttc aggctccaat tccttgacgg
     3181 caaacttctg caacgtatac cggccccgat gttccttgat ctgcaccagt ttgatgctgc
     3241 tcgaaccgat gtcgagcccc agcaactgcc gctgcggaga aaacatcgag agaaattcca
     3301 tatccgccag atttttcagt gaagccagca tgctcatgcc ctctctacgc gacctctttt
     3361 cgtccaccac ccatcacgtg cctgatttgt tcgacatttt cggcggtata cagacgccaa
     3421 tttcgccagt cgcggggtgg tccggtgatc aatccttcac gctcccagcg aaataacgtc
     3481 gcgcgcgaga tgtcgaacat ctcgcagacc tcgttggtct tgtacaattt ctttttgggg
     3541 acatcgctga ctttcttcac gaaccgcctc aaacgccgct attcatcgca tgtttcgagc
     3601 ccacggaatt aagtatagtt gatatagtcc acctgtcaag aaagtgggct ctccaggggc
     3661 ttctcgggaa gaaacggcga aacagcgctc aggaactagg gtttttgcat gtcgcggtgg
     3721 gtaacggaca catcataacc gagggcggca aattgttccc ggaggtgcac cgccatggtc
     3781 acagaccgat gacgccctcc ggtacaaccg atgccgatgc tgaggtagct gcgctgatcc
     3841 cgctcataga gcgggaccag aaagccgaac aagccttgca gatgttcgag aaattgaccg
     3901 gccgcgggat ctcccaatac atactgctgt acgcgggcat cctccccggt caatgctttg
     3961 agctcaggca cgaagaacgg attgcgaatg aaacgcacgt cgaagagcag gtcgatgtca
     4021 tagggcacgc cgaacttgta cccgaatgtg accagtgaga tggtcatgcg gcgagcggcc
     4081 gtttcctggc ggaactgccg gatcagcaac tcgcgcaatt catgcaccgt aatgttggag
     4141 gtgtcgataa cccggtcggc atgcttccgc aattcgctga ggcgctcacg ctcgaaccgc
     4201 acgccttcca cgactggcat gtgcggcaac aagggatgcg gacggcgact ctcggaaaac
     4261 cggcgaacca gcacttcctc tcgggcttcc agaaacacga gttgcagcgc atgtccctgc
     4321 gcttttagct cgcccaggac cttcgccaaa tccccgaaaa agactcgctc gcgaatatcg
     4381 atgcccaggg cgacattctt aatctccccg ccctgctgat ggcataactc gacaaaggtg
     4441 ggaagaaggg ccggcggaag attgtcgacg cagaaatacc ccgcgtcttc gaaggctttc
     4501 aaggcatggc tctttccgga tccggaaagg ccgctgatca cgacgagatt aagcgcggcc
     4561 atgagcagtg cgagcacggt cgctcgcggg atcgatcaga gacactcgtc cattcggcaa
     4621 gcgctggaga acctgaaccg tgccggacag cgcattcaca aagaccagca ccgcgagctt
     4681 cgcagaattg agcgcctcga cagcctcttc caccgtcaac acttcgagcg tcggtcgaac
     4741 cacttcgacc tcattgcgcg cagcccgtgg agccttccgt gcgaccgccc gcacacgtgg
     4801 ttgatcgccg aatttgtggt tgaccttgcg ctctttcaat ttccgcaact gggcgccgag
     4861 tttatcgacg acctcatcga tcgaggcata catttcctcg gtcgacgtct tcgcttgcaa
     4921 acgccggctc tgcacgacac ccacgacttc agccacatga tgaaactttt ccacgctgag
     4981 cagaatctgc aaggtcccga gcttcagtcc gtagcggtcc aaccgttcca tgcgtgtttc
     5041 gatatactcc cgtagagcag gagtgactgc cacatgccga cccgtgatca tcaatcgcat
     5101 tccatcctcg tttctcaggc tgcccgcacc accgtacgtg caagcccgtt gcgacgattg
     5161 ttgtaccccc aggggaactt cctccctggt gccagacccg accagcttag aaaaacttcc
     5221 tgcgttgggt ggcggaggga atgttgtcct ccgcacgata cttcgcgacg gtgcgacggg
     5281 cgatcagcac ttgctgcgtc cgcagccgcg cagcgatctc ctcatctttg agaggcttgc
     5341 gcgcatcttc cgccgcgacc atttgccgga tcatttcgcg caccgtgagg gacgacagca
     5401 tgtccgtcgg ctgatccgcg cgctgaagcc cggcgttaaa gaaaaatttc aactccaaca
     5461 tcccctgcgg acaatacata tatttattgg ccgtcacgcg gctgatcgtc gactcgtgca
     5521 tcccgatatc ctcggcgact tgcttgagaa ccaacggttt caagtgctgg atcccgcgct
     5581 cgaaaaaacc ttcttgaaac ttcacgatgc tcgacacgac cttcacaatg gtcttgttgc
     5641 gctgttcgat actccgaatc acccactgcg ccgcccgcaa tttgtcgtcc agataggcct
     5701 tggtctccgc cgaccccgat tgccctgacg acatcaattg cttgtagtac gggctgatcc
     5761 gcatacgcgg caggccgtcg tcattgagca acacttccca caccccttcg ttcttgacga
     5821 caaagacatc cgggacgatc gcgtaattct gtgtgttgga gaagggacgt ccaggcttcg
     5881 gctccaaccc ttcgataatc cgggtcgctt cgaaaacgtc gtccatcgaa atattcagcg
     5941 ccttcgcgat gcggttgtac tgtttcttct ccagatcctt cagatggtgg agcacgatgg
     6001 attccagcac cgtccccttc agcactcccg ggcgcgaccc taaggagcca atctggctcc
     6061 gtcccaaaaa cttcagttga agcagcagac attcggataa gtcccgtgcc gccacaccat
     6121 ttggatcgaa tccctgaaca tctttcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnntcg gcgttttccc cgctttcggc aggcttatcg tcgccctctc ctcgcacttc
     6301 actggatacg gtggtcgacg tatcgggctg ttctcgctcc gccgtcgccg tctcctcact
     6361 ttcttccgtc tccgtcgcca attcatccaa caacggattt tccatgaggt gctgctgcag
     6421 actttgctga agctccaaac gagacagttg cagcaatttg atcgcctgct gcaattgcgg
     6481 cgtcataatc agtttttgac tgagcctgag atccagtcgc agcttcatcg cacactcact
     6541 ccctgccgct acaggcggaa tccttcgccc aaatacacgg ccctggccat ttcacttttc
     6601 tcgattaccc cgggcgggcc cgcttccagg atcgtgcctt cattgatgat gtaggctcga
     6661 tcggtaatcg acaacgtctc ccggacattg tggtccgtaa tcaacacgcc gatattccgt
     6721 tgcttcaacc ggataatgat ttgctggata tcggccaccg caatgggatc gataccggca
     6781 aaaggctcat ccagcagcat gaatgagggg ctggtcgcta atgctcgcgt gatctccaga
     6841 cgtcgacgtt ctccacccga caaggcatag gccttacttg tgcgaatatg caccagatcc
     6901 agttccttga gcaatgtctc gacccgctcc atgcgctcac ttctcgaata gcccagcatc
     6961 tccagaatcg caaggatatt gtgttccact gacagccggc gaaacaccga cgactcctgc
     7021 ggcaaatacc cgatgcccct ccgggcccgt ttatacatgg gcaaatcggt tatttcttgg
     7081 ccgcttaagg caatcgtgcc ttcatccggc tgacacagtc ccaccatcat atcgaagatg
     7141 gtcgtttttc ctgctccatt gggcccgaga agaccgacga cttcgccggc ctgcacatcg
     7201 agcgacacac cctttaccac ttttcgtccg cgaaaactct tcaccaggcc gttggcagcc
     7261 aggcgatccg gcatgcccgt tgatgtcgct ccctgggcga ccgatgtctg ggcgtcggaa
     7321 gacatcggga tcaccgcttg ccaccgcttt cgccctcgat gaccacctga gagtccccct
     7381 ccaccacact gcgatcctcg tccaggaaca taatgatccg cttgccggat acgcgcgtcc
     7441 ccttctgcca agccaccgga tcacctgtca gaatgatttt cttttcatct tcgtagtaca
     7501 cggccttctg acaggtcgcg cgcccatctt ctttctcaat tctcactcg
//
LOCUS       DAOQRM010000120         7509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_9485_length_7509_cov_16.575530, whole genome shotgun sequence.
ACCESSION   DAOQRM010000120 DAOQRM010000000
VERSION     DAOQRM010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9485_length_7509_cov_16.575530"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PKK81_09705"
     CDS             <1..188
                     /locus_tag="PKK81_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33196.1"
                     /translation="EEGSEETSEEGSEEEVSPTRIYTPGVRPLTSGVFLCLKAAMALW
                     GIGIGVYLRMRVLGSSG"
     gene            complement(185..1111)
                     /gene="lpxC"
                     /locus_tag="PKK81_09710"
     CDS             complement(185..1111)
                     /gene="lpxC"
                     /locus_tag="PKK81_09710"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNV33197.1"
                     /translation="MRFQQTIGSPVSCSGVGLHSGQPVTLTLRPAPPNTGIVFVYRNG
                     SEETLLPAAVSNKVPTELCTAISVNGRQVKTIEHLLSALVGMEVDNVYAEVDAGEVPV
                     LDGSASPFVRLIRAAGVIPQTRRQSYVKITQPIEVVDGARRVRIEPSSTPKITYSIHY
                     DHPLIQTQSYTYTCSASAFEQDIATARTFGFLHEVEALWARGLGKGGTLDNTVILSKD
                     GVVNESGLRFQNEFVRHKVLDLIGDIALLGFPFIGHIVAERSGHAMHTRLVEQILAQR
                     DKWALITGEHAVAASESRSPLGLLRPAPSLAI"
     gene            complement(1395..2066)
                     /locus_tag="PKK81_09715"
     CDS             complement(1395..2066)
                     /locus_tag="PKK81_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV33198.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKTKDDINVVGEAQDGVEAVEK
                     TKTLMPDVILMDVSMPRMGGIEATRQIKREFPHMGIVALTMYEEQQYIFDLVRAGATG
                     YLLKDSESSQIVAAIRAIYRGESLIHPSVASKILAEFSLMSQKKGKKPAWVEHDLTER
                     EITVLRLVADGKTNKEIANNLDLSEKTVKNHVRNIFHKLQVYDRTQAAILAIRKGLIE
                     LEPRP"
     gene            complement(2053..3057)
                     /locus_tag="PKK81_09720"
     CDS             complement(2053..3057)
                     /locus_tag="PKK81_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNV33199.1"
                     /translation="MEIFANDPLVRIVGVADISDQAPGLGLAKRLHIRVTRNYRQLLK
                     MGPVDLVIDVSGNPEVGEYLQDIRRMGVSVIGGASAKFMWQLIEARIRATADIEKALN
                     KYQSLYRLYVKETGAAVTEERTRIACEIHDGLVQSLAGVNFKLELSQELIRKNPKASL
                     ATIRESKAQLKLAIQEARQVIFNLRPLHYDKMELIPALTNYLKSYETQYHIKTAFSVT
                     GDETILFPRTKIFLFRIVQEALSNVQKHAKAGRVSVQLDIRFDLLQVTISDNGIGFDM
                     DAVLRDPEKWDHFGIRGILERARLVGGEATIDSKKGRGTRIVLRIPLADKETIRHGKN
                     "
     gene            complement(3283..3747)
                     /gene="nrdR"
                     /locus_tag="PKK81_09725"
     CDS             complement(3283..3747)
                     /gene="nrdR"
                     /locus_tag="PKK81_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNV33200.1"
                     /translation="MKCPFCDDVEDKVVDSRMAKEGEVIRRRRECLSCKRRYTTYERV
                     EETMPAVVKKDGRREPFDRSKIVSGLKKACEKRPISTATIETVTDRIEKRIQDLGETE
                     IVSTAVGEEVMRELSQLDQVAYVRFASVYREFKDIDQFMDEIKALAQQRRER"
     gene            complement(3763..5040)
                     /gene="glyA"
                     /locus_tag="PKK81_09730"
     CDS             complement(3763..5040)
                     /gene="glyA"
                     /locus_tag="PKK81_09730"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNV33201.1"
                     /translation="MQELIGSLDALKSTDPETYEAIVAEEQRQRDKLLLIASENFASP
                     AVLAAQGSVMTNKYAEGYPGKRYYGGCQHVDTVESLAIERAKQIFGAEHVNVQPHSGS
                     QANMAAYLSVLKPGDTILGLDLAQGGHLTHGSKVNFSGIIFRAFSYGVDRQTETIDYA
                     AVQKIAEECRPRMLVVGASAYARTLDFPKFQEIAKSVGAYLLVDIAHIAGLIAAGLHP
                     NPVPYADFVTTTTHKTLRGPRGGVTMCKAEYAKAVDKIIFPGLQGGPLMHVIAAKAVA
                     FKEALSPAFKRYQQQVLANARTLAQGLVDRGYKIVSGGTDTHLMLVNLTNKGITGKEA
                     DAALDAAGIIVNKNAVPYDEKPPATASGIRIGSPIVSTRGMREAEMREIVALIDRVLQ
                     HPQDQQVQAEVRAQAKTLCNRFPIFHAYDSSPA"
     gene            complement(5095..5589)
                     /gene="rplI"
                     /locus_tag="PKK81_09735"
     CDS             complement(5095..5589)
                     /gene="rplI"
                     /locus_tag="PKK81_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNV33202.1"
                     /translation="MKVILQETLEGVGDLGDLLDVSNGFARNYLLPRKKAVEANSRNI
                     KEFEHAKRAAAEKAKKEKQDIEAHGKKISAVSLTVAAQVGKDDKMFGSVTAKDIAEGL
                     AEQGFTVDRRKIQLAQPIKELGTFAIAIKLPREVTATIAVHVVKKQDEQEAQAEPEEA
                     APTA"
     gene            5891..7366
                     /locus_tag="PKK81_09740"
     CDS             5891..7366
                     /locus_tag="PKK81_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33203.1"
                     /translation="MTAIPDTLPDLLDQLAATLDGDAARGTEQGLDQSIPLTRGRLLQ
                     TVGNLHLYEFYLSADVVVGADLSATLLLGEEAEPTEGIVLFQDGERLVLQLFDAIGDV
                     VPSATLVPDASGLALTTSRRLAEMSKTGASYTLGPAERLLPVLEAGQTGDRAALEALV
                     PTSVLTPLWQDDLTARRQKLATLIIELLRGNKRILLVAPDHESADAITLLTARAMKAA
                     GLTFKSWLSRYEVATSKESGGIPLPDLGFEAQMHQFYAKSRSDKAVLRKKYDRFRELT
                     PILAYKAQKQKDLDEVRLLEWRLVTQLTDFLAKIKEIDQTLTDYEQLPIWKRLSMQAV
                     GKNVESLREYKAIYERHCAGLRHELDIAKERIAELIPEATVPKELKPEFAELKEEVIR
                     LGGTKKIRELLAAQEDTNRQAFVQNRRVIVTTAARVAADPLFRKVRFDVLIADEAPRI
                     RAAFLLAAAGLVRERIVLSGDLREVTAGGAWNLTDGVLTAG"
BASE COUNT         1420 a         2105 c         2304 g         1680 t
ORIGIN      
        1 acgaagaagg cagcgaagaa accagcgaag aaggcagcga agaagaagta agtcccacca
       61 ggatttacac gccgggggta aggccactta cctccggcgt ttttttatgc ctaaaagcag
      121 ccatggcctt atggggcata ggcataggag tgtatctgcg tatgcgggtg cttggaagca
      181 gcggttagat cgcgagggat ggtgcggggc gaaggagccc gagcggagag cgagattcgg
      241 atgccgcgac tgcatgttcc ccggtgatga gggcccattt gtcgcgctga gccaggattt
      301 gctcaacgag tctggtgtgc atggcatggc ctgatcgttc cgcaacgata tggccgatga
      361 acgggaagcc gagaagcgcg atatcgccga tcaagtccag gaccttgtgg cggacgaact
      421 cgttctggaa gcgcaggccc gattcattca cgacaccgtc tttggacaga atcacggtgt
      481 tgtccagtgt cccgcctttt ccgaggcctc tggcccacag ggcttcgact tcgtgcaaaa
      541 atccaaacgt gcgagctgtg gcgatgtcct gctcaaaagc cgaggcggaa caggtatagg
      601 tgtaggactg ggtttggatc aatggatgat cgtagtgaat ggagtacgtg atcttcgggg
      661 tggaagacgg ctcgattctg actcggcgtg ctccatcaac aacctcaatg ggttgcgtga
      721 ttttcacata cgattggcga cgggtctggg ggatcacgcc ggccgcacga atcaatcgca
      781 cgaaggggct cgcgctgcca tccagcacgg ggacttcccc tgcatcgact tctgcgtaca
      841 cgttgtcgac ttccatgccg accaaagccg acaggaggtg ttcgatggtc ttcacttgac
      901 ggccgttgac gctgatggcc gtgcagagct cggtcggtac tttatttgag accgcagccg
      961 ggaggagggt ttcctccgat ccgttgcgat agacaaacac gattccggta ttagggggag
     1021 ctgggcggag tgtcagcgtg accggttggc ctgagtggag cccgacgccc gaacatgaaa
     1081 ctggtgatcc gatcgtttgc tgaaaccgca cggtgcctcc tccgtaatgt gtactctcat
     1141 atgagcaatg cttgtgccaa gcattaaatt aatcgtaaga tgttgatata tattgaatta
     1201 tattgaatgg ttgaaagtgc tcactgtggt gaaattcaca caattgtatc ttttgatgga
     1261 agaggcgaag cacggaagtg tttggttttc aatgagttaa ctgattgtat caggctgggt
     1321 ttagcgggaa ccacgtattc cggcatcaat atcgagatcc gtgccggggc tttgcattgg
     1381 tggcgcggcc tccgttaggg tctcggttcc aattcgatca gccctttgcg gatggccaga
     1441 atcgcggcct gggtccggtc atacacctgc agtttgtgaa agatattgcg gacgtggttt
     1501 ttcaccgtct tctcgctgag atccaagttg ttggcgattt ctttgttggt cttcccgtct
     1561 gcgacgaggc gcaggaccgt gatttcgcgt tcagtcaaat cgtgttcaac ccaagccggc
     1621 ttcttgccct tcttctggga catgagggag aactcggcca ggatcttact ggcgaccgac
     1681 ggatgaataa gcgactcgcc ccggtagatc gcccggatgg ccgccacgat ttgtgaggac
     1741 tcagagtctt tcaacagata cccggtggcg ccggcgcgca cgagatcgaa gatgtattgc
     1801 tgctcttcat acattgtcag cgccacgatg cccatgtgag ggaattcccg cttgatttgc
     1861 cgggtggctt cgatgccgcc catgcggggc atgctgacat ccatgagaat gacgtccggc
     1921 atgagagttt tggttttctc cacggcttcc acgccgtctt gcgcttcgcc caccacgttg
     1981 atgtcgtctt tggtcttgag gatggccgcc aggccttcgc gcaccacgcg gtgatcatca
     2041 gcgatcagta ccttaatttt ttccatgacg aatcgtctcc ttgtcggcta gtgggattcg
     2101 cagcacgatg cgcgtgccgc gccctttctt cgagtcgatc gtggcttccc cgcccaccag
     2161 tcgtgcgcgt tccaggatgc cgcgaatgcc gaagtgatcc cacttttcgg gatcgcgaag
     2221 gaccgcatcc atgtcgaatc cgatcccgtt gtcggaaatg gtcacttgca acagatcaaa
     2281 ccgaatatcg agctgcaccg acacccggcc ggccttggcg tgcttttgca cgttgctcag
     2341 ggcttcttgc acgatccgga acaggaagat cttggtgcgc gggaacaaga tcgtttcgtc
     2401 tccggtcacg gaaaaggccg tcttgatatg gtattgggtt tcgtaggact tgagatagtt
     2461 cgtcagtgcc gggatgagct ccattttgtc gtagtggagc ggacgcaggt tgaagatgac
     2521 ctgcctggcc tcctgaatgg ccagtttcaa ttgggctttg gattcacgga tcgtggcgag
     2581 actggctttg ggattcttcc gaatcagctc ttgagagagc tccagcttga agttcacgcc
     2641 ggcgaggctc tgcaccaacc cgtcgtgaat ttcgcaggca atgcgcgtcc gttcctccgt
     2701 caccgcggcg ccggtttctt tgacgtacaa gcggtacagc gactggtact tattcaaggc
     2761 tttttcgatg tcggccgtgg cgcgaatgcg cgcctcgatc aactgccaca tgaatttggc
     2821 gctggccccc ccgatgaccg agacgcccat gcggcgaatg tcctgcaggt attcgccgac
     2881 ctccgggttg cccgacacat cgatgaccag atcgaccggc cccattttca gcagttggcg
     2941 atagttgcgt gtcacgcgga tgtgcagccg tttggcgagg ccgaggccgg gtgcctgatc
     3001 gctgatgtcg gccacgccga cgatgcggac caagggatcg ttggcgaaaa tctccattag
     3061 ggcagtgccg ccgcggcccg cgccgatgat aaccacgtgt gtggcccccg gcgatcggcg
     3121 tcggcttggt cgcttggctg ttcgtcggtt gcgggtgact ttggtcgttg ccatgggttc
     3181 agagcctatc gggagtgcgc aggtctctcc tctgctggtg cccatcctac cgagacctac
     3241 accgagcgga ccgatccgaa acagcgaaga gaaggggagg gcttaacgct cccggcgctg
     3301 ctgggccagg gctttgatct cgtccatgaa ctggtcgatg tctttaaatt cccgatacac
     3361 ggaggcgaat cgaacatagg cgacctgatc gagttgggac agttctctca tgacttcctc
     3421 accgacggcg gtgctcacga tctcggtttc acctaaatcc tggatgcgct tttcgatgcg
     3481 atcggtgacg gtttcgatgg tcgcggtgct gatcgggcgt ttttcgcagg ctttcttgag
     3541 cccggacacg atcttgctcc gatcaaacgg ctcccgtcgt ccgtcctttt ttacgacggc
     3601 gggcatggtt tcttcgaccc gttcgtaggt ggtgtaccgg cgtttgcacg acaggcattc
     3661 gcgccgccgc cggatgacct cgccttcttt ggccatccgc gaatcgacga ctttgtcctc
     3721 gacatcgtcg cagaaaggac acttcactgg cgaacgtcct gcctaagcgg gagacgaatc
     3781 gtaggcatga aagatgggaa aacgattgca caacgttttt gcttgcgccc gcacctcggc
     3841 ctgcacctgc tgatcctggg gatgctgcaa cacacgatcg atcagcgcca cgatctcacg
     3901 catctctgcc tcgcgcatgc cgcgcgtcga gacgatgggg ctgccgatcc ggatgccgct
     3961 ggccgtggcc ggcggttttt cgtcgtaggg gacggcgttc ttgttgacga tgatgccggc
     4021 ggcgtcgagg gcggcgtcgg cttctttccc cgtgatgcct ttgttggtga ggttgaccag
     4081 catcaggtgg gtatcggttc cccccgacac aattttatag ccacggtcga cgagtccctg
     4141 cgccagcgtg cgcgcgttgg cgagcacctg ctgctggtac cgcttgaacg ccggcgacag
     4201 cgcttccttg aatgcgacgg ctttggcggc aatcacatgc atgagggggc cgccctgcag
     4261 gccggggaag ataatcttat cgacggcctt ggcgtattcg gccttgcaca tggtgacgcc
     4321 accgcgcggg ccacgcagtg tcttatgcgt cgtggtcgtg acgaaatccg cgtaggggac
     4381 gggattggga tgcaacccgg cggcgatcaa tccggcaatg tgcgcgatat cgaccagcag
     4441 gtacgcgccc accgacttgg cgatttcctg aaacttgggg aagtcgaggg tgcgggcgta
     4501 ggcgctggcg cccaccacca acatgcgggg acggcattcc tcggcgatct tctgaaccgc
     4561 cgcataatcg atggtttcgg tctggcgatc gacgccatac gaaaacgcac ggaaaatgat
     4621 gccggagaaa ttcaccttgc tgccgtgggt gaggtgtccg ccctgggcga ggtctaatcc
     4681 cagaatcgtg tcgcccggct tgagcacgga caggtacgcg gccatattgg cttgcgatcc
     4741 cgaatgcggc tgcacgttga cgtgctcggc gccgaagatc tgtttcgcgc gttcgatggc
     4801 gaggctttca accgtatcga catgctggca accgccgtaa tagcgcttgc cgggataccc
     4861 ttcggcatac ttattcgtca tcacgctgcc ctgggcggcc aagacggcgg ggctggcgaa
     4921 attttccgag gcgatcaaca gcaacttgtc ccgttgccgc tgctcctcgg cgacgatagc
     4981 ctcgtaggtt tcagggtcgg ttgatttcaa tgcgtccaac gaaccgatca gctcctgcat
     5041 gcttcctccg agcgcagcct tctatccggt ggccacgcat gatcccgcaa cgggttatgc
     5101 ggttggtgcg gcttcttccg gctctgcttg cgcttcttgc tcgtcttgct tcttcaccac
     5161 atggaccgcg atggtcgcgg tgacttcgcg cggcagcttg atggcaatgg cgaaggtgcc
     5221 gagttccttg atgggctggg ccagctggat cttgcggcga tccacggtga atccctgctc
     5281 agccaaccct tccgcgatat ccttggccgt tacggatcca aacatcttgt cgtcttttcc
     5341 gacctgggcg gcaacggtca gcgagaccgc ggagatcttc ttgccgtgcg cctcgatgtc
     5401 ctgcttttcc ttcttggcct tctcggccgc ggcccgcttg gcatgttcga attctttgat
     5461 gttccggcta ttggcttcaa cggccttctt gcggggcaag agatagttcc gggcaaaccc
     5521 gttggagacg tccaaaaggt cgccgaggtc gcccacgcct tcgagtgttt cttggagaat
     5581 caccttcata ctgcaactcc ttctgttgga aagggaaaaa gcatactggc gggctgtgca
     5641 aaagtcaatt gatctggagg catgcgacgg ttttcttcat tcggctcgaa atggcggcgc
     5701 aggcgttcag tgcggctctt tccgttcatt gtcgcgtttc agcggtggat tgtttgtgcg
     5761 tgcgtcgctg gtggacagga tctttcggaa ctctttaaga tacgttcccg attctatgaa
     5821 caatgcgctg acggttgtgc gaccggcgtc gcaggctgcg ccacgacgac acgcagggga
     5881 agaacggaac atgaccgcaa taccagacac cttgcccgat ctcctcgatc agctggcggc
     5941 gacgctggac ggtgatgccg cgcgcggcac ggagcagggg ctcgaccaat cgattccgct
     6001 cacgcgaggc cggctgctcc agaccgtcgg gaatttgcac ctgtatgagt tctatctctc
     6061 cgccgacgtg gtggtgggag ccgatctgtc tgcgactctg ctcctgggcg aagaggctga
     6121 acccacggag ggcattgtgc tgtttcagga cggggagcgc ctcgtgctgc aattgttcga
     6181 tgccatcggc gatgtggtac ccagcgccac gctggtgccg gatgccagtg gattggccct
     6241 gaccaccagc cgccgcttgg cagaaatgag caaaaccggc gcctcctaca cactcggccc
     6301 ggccgaacgg cttctccccg tcctcgaagc gggacaaacg ggggaccgcg ccgcgctgga
     6361 ggccctggtg cccacctccg tgctgacgcc cttgtggcaa gacgatctga cggcgcgtcg
     6421 gcagaagctg gccacgctca ttattgagct gcttcggggc aacaaacgca ttcttctcgt
     6481 tgctccggac cacgagagcg ccgatgcgat cacccttctc acggcccggg cgatgaaagc
     6541 agccggactg acgttcaaaa gttggctgag tcggtacgaa gtggcgacca gcaaggagag
     6601 cggaggcatt cctcttccgg atttgggatt cgaggcgcag atgcatcagt tctatgccaa
     6661 gtctcgatcc gacaaggcgg tcttgcgcaa aaaatacgac cggtttcgcg aactcacgcc
     6721 gattctggcc tacaaggctc aaaagcagaa ggatctggat gaggtgcggt tgctggaatg
     6781 gcgactcgtg acccagttga ccgatttcct ggccaagatc aaggaaatcg accagaccct
     6841 gaccgactat gaacaactcc ccatctggaa gcggttgtcc atgcaggcgg taggaaaaaa
     6901 cgtcgaatcg ctgcgtgagt acaaagcgat ctatgaacgg cactgtgccg ggcttcgcca
     6961 tgaactcgac atcgccaagg aacggattgc cgaattgatt cccgaggcga cggtcccgaa
     7021 ggagctcaag ccggaatttg ccgagctcaa agaagaagtc atccggttgg ggggcacgaa
     7081 gaagattcgc gagctgttgg cggcgcagga ggatacgaat cgacaggcct tcgtgcagaa
     7141 ccggcgggtc atcgtcacga cggcggcgcg ggtggcggct gatccgttgt ttcgcaaggt
     7201 gcggttcgac gtcttgattg cggatgaggc gccacgcatc cgcgcggcct ttctgctggc
     7261 ggcggcgggg ttggtccgcg aacggattgt gctcagcggc gacctgcgcg aggtgacggc
     7321 cggtggcgcc tggaatctca ccgacggggt cctgaccgcc ggctgaactc gattcacctc
     7381 agccgacgga tattttcatg ttcctcacct tgacgggtcg aaaccgatca gcgataatcc
     7441 cactccattc cttaggccga agtggcggaa ctggcagacg cactggtttc aggtaccagc
     7501 gcccgtaag
//
LOCUS       DAOQRM010000121         7490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_9522_length_7490_cov_14.757095, whole genome shotgun sequence.
ACCESSION   DAOQRM010000121 DAOQRM010000000
VERSION     DAOQRM010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9522_length_7490_cov_14.757095"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /gene="pxpB"
                     /locus_tag="PKK81_09745"
     CDS             <1..681
                     /gene="pxpB"
                     /locus_tag="PKK81_09745"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HNV33204.1"
                     /translation="PRIVPLSESALTIEFGETIDPHTNESVLAFATAVERAGLPGILE
                     VVPTYRSATVYFDPFRTDVATMTGHFRALMTATNTPPLRAPTTHTIPVWYGGSAGPDL
                     GDVAKRAALMPEQVAALHASVLYRVYMLGFSPGFPYLGTVPDRIATPRLPTPRKQVAA
                     GSVGIAGTQTGIYPHASPGGWRIIGRTPVPLFSLTRPAPFLLAPGDQVQFVPIEETEF
                     HALFENHT"
     gene            678..1454
                     /locus_tag="PKK81_09750"
     CDS             678..1454
                     /locus_tag="PKK81_09750"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNV33205.1"
                     /translation="MNKERSVDLNCDLGEAATPEQLDVEARIMPYVTSVNIACGVHAG
                     DAALMRSTVQLARQHDLAIGAHPGLPDRDSGGRREQPLSRSLVQDLILSQVGELMAIS
                     QAEGLRLSHVKPHGALYNMSARDPALADAIAEAVAHLDPRLILVGLAGSELLVAGKAR
                     GLPVAAEGFADRAYQADGRLVPRGQEGALIHDESTVVSRAHSLIHDDTIAAIDGSSLH
                     LHIDTLCVHADTPGAVRLAHALRTMFDDGGISAVRIDHAS"
     gene            1444..2409
                     /locus_tag="PKK81_09755"
     CDS             1444..2409
                     /locus_tag="PKK81_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HNV33206.1"
                     /translation="MPLKQPTIHVVRPGLFTTIQDLGRFGYQRFGVSVSGAMDPWALT
                     VGNRLLGNPDRAAGLEVTIQGPELLFEDALSIVITGADLSPTSNGVALPMWTVVRMPA
                     GSRLEFGIRRQGTRAYLTVAGGIDGPLMLGSRSTHVRSGLGGVAGRALRKWDRLDVGP
                     ARAAGKPCVGRALPEAQRPRYQSTATIRVIPGPQADRFTDDALERLASQPYRLASESD
                     RMGYRLQGLALPHRTSADIVSDAVSAGNIQVPADRQPILLMADCQTTGGYAKLATMIA
                     ADRALAAQLSPGDRLSFVVVTHETASTLLRSAHAELDRLLPPHRA"
     gene            2543..2743
                     /locus_tag="PKK81_09760"
     CDS             2543..2743
                     /locus_tag="PKK81_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33207.1"
                     /translation="MDTTLTPRQITHDEKKAAEAAFAGRPFNPKWSDAARIVYDGLLA
                     AMGKDARTVDMHDELEPVEPLA"
     gene            complement(2855..5530)
                     /locus_tag="PKK81_09765"
     CDS             complement(2855..5530)
                     /locus_tag="PKK81_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV33208.1"
                     /translation="MDLITTHLNADFDGLASMVAARKLYPGAVLVLPGGAQESVRGFL
                     ATHHLDISRLKDVALERVRRLILVDVQEPERLGPLKTLSSRADVEVHVFDHHGADDEP
                     EPQTRPQWPHLAERHIEPVGATTTLLVERLKAQQIILTAAEATVLALGLYEETGSLAY
                     PSTTPRDLEAAAVVLRAGADLNVVAEVLRHPLDPDLIALLNDLLQSGSIYYLEGRKIL
                     VASSTYDRYKGDLAEAVHRLAELQGFDAVIVAIALDEKVEVIGRSRRPEIDVAWIARE
                     FGGGGHAVAAAASVKGRTLIEVQERLTRLLTERYRPTLVARDVMTQPVKTIVADATVA
                     EAERSMTTYGVNVLPVVDQKDRYVGTISREVVQKALFHRLGAQRVEDLARHDQYSADP
                     ETPFHDIEMRMIELNQRFVPVLCGGKTVGVITRTDLLRSLHEDVIASARGKAKSLMDL
                     ESSGAVRRRDVGGLLRDRLPREVHDLLQTAGDLGERLGYSAYVVGGFVRDLLLGIDNL
                     DVDFVVEGDGIAFARALAKERAGRVKVHERFGTAVVLLPDGFKVDVATARTEYYEYPT
                     ALPTVEQSSIKKDLYRRDFTINTLAVRLNPRAFGQLIDFYGGQRDLKERLIRVLHSLS
                     FVEDPTRVFRAIRFELRFDFHLSKETLALIKGAVKMELFHRLSGQRLLDELRYLFSER
                     TPRHAVRRLADLDLLRFIHPTLTWSNRLDRRLSEVEAALDWYKLSCLDRQISGWLVYA
                     MALTEAMPDQAVREMIERFPFTEAEREAITAARFLTQPVCRALSRRVPLRPSETVALL
                     AGLADETLVFLLAKNGSASAKRHLSLYLTTYRDVKPALTGKSLQALGFRPGPLYRTIL
                     ERLTEARLDGDVKTEAEERELVKTFSDQGRGRARD"
     gene            complement(5753..6070)
                     /locus_tag="PKK81_09770"
     CDS             complement(5753..6070)
                     /locus_tag="PKK81_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33209.1"
                     /translation="MKQVMWGAALLSVCATSLVFAQAETPRIDQRQANQEKRIDQGIA
                     SGQLNEREATRLNNQQEHVNKLEDKAKSDGVVTKKERARINHAQDRTSRHIARQKHDR
                     QQR"
     gene            complement(6214..6912)
                     /locus_tag="PKK81_09775"
     CDS             complement(6214..6912)
                     /locus_tag="PKK81_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNV33210.1"
                     /translation="MISVVIPAYNEERALPATLRQLFEQAGSYEVIVVDGGSHDRTCE
                     IVRRWPINSRLPAPLTLVTAPKGRASQMNAGAKLARGEWLLFLHADTLLPVDALSRIN
                     ALERNLTIQAGGFRHRFSGSDWRLRMISWLDNFRCVRSHIIYGDQALFVRRVLFERLG
                     GFPDRPILEDVAFGENLLEVTTPLLLSPPVVTDARKFLKMGIWRSFLRVLLVILHVEF
                     KLPYLPRAFFQDIR"
     gene            complement(6909..>7490)
                     /locus_tag="PKK81_09780"
     CDS             complement(6909..>7490)
                     /locus_tag="PKK81_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNV33211.1"
                     /translation="EHGERMAHGCAQRQATLAPDPKARRFLTTQARQEAMHAVVFRGA
                     IAWLAPRHLGDAPFLPALEDYRRLLAEALERGDWLESILAEQVILEGLGEAILTRIEG
                     GLEKRQAPFRRLRRVLLHQEEAHHDFGRRVLERAMVDGHRELESLRLRTQDYLALTDS
                     MVLTLCDLFETIDEDASAWAADVRTFLPMWLTE"
BASE COUNT         1512 a         2439 c         2126 g         1413 t
ORIGIN      
        1 ccgcgcatcg tgccactgag tgagtccgcc ctgaccattg aattcgggga gaccattgac
       61 ccgcacacga atgagtcggt cctggccttc gccaccgccg tggaacgggc cgggctcccg
      121 ggcattctgg aggtggtgcc gacctaccgt tccgccacgg tctatttcga tccgttccgt
      181 accgacgtgg ccacgatgac cgggcatttc cgtgccttga tgacggcaac gaacacaccc
      241 ccgctacgtg cccccacgac tcataccatt cccgtgtggt atggaggtag cgcaggaccc
      301 gatctgggcg acgtggccaa acgagcggca ctcatgcccg agcaggtggc ggccctgcat
      361 gcttccgtgc tgtaccgcgt ctacatgctg ggattcagcc cgggctttcc ctatctaggg
      421 accgtgccgg atcgcatcgc gacgccgcga ctcccgacgc ctcgaaaaca agtcgcagca
      481 ggatcggtcg gcatcgccgg cacacaaacc ggcatctacc cgcatgccag cccgggcggc
      541 tggcgcatta tcggtcgcac tccggtaccg ctgttcagcc tgaccagacc cgcgccattt
      601 ttgctggcac cgggcgacca ggtgcaattc gtgccgatcg aggaaacaga attccatgcg
      661 ctgttcgaga accacacatg aacaaggaac gtagcgtcga cctcaattgc gacctgggag
      721 aagccgcgac accggagcaa ctggatgtgg aagcccggat catgccctac gtcacatcgg
      781 tgaatatcgc gtgcggggtg catgcaggcg atgcggcctt aatgcgaagc accgtgcagc
      841 tggctcgaca gcatgatctg gcgatcggag cgcaccccgg attgccggat cgggactccg
      901 gcggccgccg tgaacaaccg ctctcccgtt cgctcgtaca ggacttgatc ctgtcccaag
      961 tgggtgagct gatggccatc agtcaggccg aaggtcttcg gctctcgcat gtgaaaccgc
     1021 acggggcgct ctacaacatg tctgctcgcg accccgcgct ggccgacgcc atcgccgaag
     1081 cggtcgcaca ccttgatccg cgattgatcc tcgtgggatt ggccggatcg gagctcctcg
     1141 tcgccggcaa ggcccgcggg ctcccggttg ccgccgaagg atttgccgat cgggcttatc
     1201 aggccgacgg ccgtttagta cctcgagggc aggaaggcgc gctcatccac gatgaatcga
     1261 ccgtggtctc gcgcgcacac tcgctgattc acgacgacac catcgcggca atcgacggtt
     1321 cctcgcttca tctacatatc gacacgcttt gtgtccacgc cgacactcct ggcgcggtcc
     1381 gactggctca tgcgctacgg acgatgttcg acgatggagg gatttccgca gtccgtatcg
     1441 atcatgcctc ttaagcagcc gaccatccac gtcgtacgtc ccggactctt cacgaccatt
     1501 caggatctcg gtcgttttgg ataccaacgg ttcggcgtgt cggtgagcgg cgcgatggat
     1561 ccctgggcgc tcacggtcgg gaaccgtctc ctcggcaatc ccgaccgggc cgccggcctg
     1621 gaggtcacca tccaaggacc ggaactgttg ttcgaggacg cactctccat cgtcatcacc
     1681 ggggcagacc tctctcccac cagcaacggc gtcgcactgc ccatgtggac cgtcgtccgc
     1741 atgcccgctg ggagtcgcct tgagtttgga atacgccgac aaggcactcg tgcctacctc
     1801 accgtggccg gcggcatcga cgggcctttg atgctcggca gccgatcgac ccatgtgcgc
     1861 agtggattgg gaggcgtcgc aggccgcgcg ctgaggaagt gggatcgact cgatgtcgga
     1921 ccggcacgag ctgcaggcaa accatgcgta ggacgagcac tgcctgaggc acaacgcccc
     1981 cggtatcaat ccaccgccac gatacgggtg ataccggggc cacaggccga tcgtttcacg
     2041 gacgatgcgc tggagagact cgcgagccaa ccgtaccgac tcgcctccga atcagaccgg
     2101 atgggctatc ggttacaagg attggcgttg ccgcatcgca cctcggccga catcgtgtcc
     2161 gatgccgtga gtgccgggaa cattcaagtg ccggcggatc gacagccgat tctactcatg
     2221 gccgactgcc agacgacggg ggggtatgcc aaactcgcga cgatgatcgc ggccgatcgc
     2281 gctctcgcgg ctcaactatc gccgggagac agactctcgt tcgtagtggt cacgcacgag
     2341 acagcatcga cactgcttcg gtcggctcac gccgaacttg accggctctt gccgccgcac
     2401 cgtgcttgag gagattccct tagtccggac gcttcaggcc atgcatgccg cagcactcga
     2461 aagcaatgcc cgctaaacaa cgggggttct gccattccgt tgagtccttg acattgccct
     2521 gaagcgatct attattattc gtatggacac cacactgact cctcgacaaa ttactcacga
     2581 tgagaaaaag gcggccgaag ccgctttcgc tggtcgccca ttcaatccta aatggtcgga
     2641 cgcggctcgc atcgtttatg acggcctcct cgccgcaatg ggaaaagatg ctcggaccgt
     2701 cgacatgcac gacgaactcg agccggttga gccgttggct taacctcccc gcgtcgtgtc
     2761 atcgggcacg ggaggcgttc agcacgacct ctcgaggttg tctgcgctct gcggcaccgc
     2821 cgatgtggct tgatacctcc ccgcatgcgg gaagctagtc tctcgctctc ccccggcctt
     2881 gatcagaaaa tgttttcacg agctcccgct cttccgcttc agtcttcacg tcgccatcca
     2941 accttgcctc ggtcagacgc tcgagaatcg tgcgatagag tggacccggt ctgaatccaa
     3001 gcgcctggag ggatttcccg gtcagggcgg gtttcacgtc gcgatacgtc gtcagataca
     3061 gagacaggtg ccgcttggcc gatgccgagc cgttctttgc cagcagaaac acgagcgttt
     3121 cgtccgccag accggccagc agagccaccg tttccgaagg cctgagcggc acgcgccggc
     3181 tcagagcccg gcacaccggc tgcgtaagga accgagccgc cgtgatagcc tctcgttccg
     3241 cttccgtgaa gggaaatcgt tcgatcatct cgcgtacggc ttgatccggc atcgcctcgg
     3301 taagtgccat ggcatacacc agccatccgc tgatctgccg atccagacag gacagtttgt
     3361 accaatccag cgccgcttcc acttcgctca gccgacgatc cagtcgattc gaccacgtca
     3421 gtgtcggatg aatgaaccgc agaagatcca ggtccgccaa ccggcgaacg gcatggcgtg
     3481 gggtgcgctc agaaaataag taccgcagtt catccaacaa ccgctggccg gacagtcgat
     3541 ggaacaactc catcttcacc gcgcctttaa tcagcgccag cgtctccttg ctcaaatgaa
     3601 aatcgaaacg cagctcgaag cgaatcgcgc gaaagaccct cgtcggatcc tccacaaaac
     3661 tcaagctatg tagtacccgg atgaggcgtt ctttgagatc gcgttgtccg ccgtaaaaat
     3721 cgatcagttg gccgaacgcg cggggattga ggcgtacagc caacgtgttg atggtgaagt
     3781 cccgacggta gaggtccttc ttaatggagc tctgctcgac cgtcggcaac gccgtcggat
     3841 attcgtaata ctccgtgcgg gccgtagcca catctacctt gaacccgtcc ggcaggagca
     3901 caacagccgt cccgaatcgc tcgtggacct tcacccggcc tgcccgctcc ttcgccaggg
     3961 cgcgtgcaaa cgcgatcccg tcgccctcca ccacaaaatc gacatcgagg ttgtcgatgc
     4021 cgagcagcag gtcacgcaca aatccaccga ccacatatgc tgagtagcca aggcgctcgc
     4081 ccaagtcgcc tgccgtctgt agcaggtcat gcacttcacg cggcaagcgg tcccgcagga
     4141 gtcccccgac atcgcgtcgg cgcactgctc cggaggactc caggtccatc aacgatttgg
     4201 ctttccctcg cgcagacgca atcacgtctt catggagact gcggagcaga tcggttcggg
     4261 taatcacgcc caccgttttg ccgccgcaca gcaccggcac aaaccgctga ttcaattcga
     4321 tcatccgcat ttcgatgtcg tgaaaggggg tctccgggtc tgcactgtac tgatcgtgtc
     4381 tggccagatc ctcgactcgt tgtgctccga gtcgatgaaa caatgccttc tgaacgacct
     4441 cacgtgaaat cgtcccgaca tagcggtcct tctgatcgac gaccgggaga acgttgaccc
     4501 cgtaagtagt catggagcgt tcggcctccg ccacggtcgc atccgcgacg atcgttttga
     4561 ccggctgtgt catgacatcc cgagccacca gggtcgggcg atagcgttcc gtcaggagac
     4621 gcgtcagccg ttcctgcact tcgatgagcg ttcggccctt cacgctggcc gctgccgcca
     4681 cggcatgtcc gccgccgccg aattcgcgcg caatccacgc cacgtcgatc tcggggcgcc
     4741 ggctccggcc aatcacctca accttctcat ccagtgcaat ggcaacgatg acggcatcga
     4801 atccctgtaa ttccgccaac cggtggacgg cctccgcgag atcgcccttg taacggtcat
     4861 acgtgctgga ggccacaaga attttgcggc cctccagata atagattgat ccggactgca
     4921 acagatcgtt cagcagggcg atcagatcgg gatccagcgg atgccgcaag acctccgcca
     4981 cgacgttcaa atccgcgccg gcgcgcagca caactgccgc agcctccaga tcgcgcggag
     5041 tcgtcgacgg ataggccaat gaccctgtct cctcgtacag accaagcgcc aacaccgtcg
     5101 cctcggcagc cgtgaggata atctgctgcg ccttcaggcg ttcgaccagc agcgtggtcg
     5161 tggcgccgac cggttcgatg tgccgttcag cgagatgagg ccactgaggt cgagtctgcg
     5221 gctccggctc atcgtcggct ccatggtgat cgaaaacatg cacctccacg tctgcgcgtg
     5281 aactgagggt cttcaatgga cccaaccgct ccggctcctg gacgtccacg agaatcaacc
     5341 gtcgcacccg ttccagggcc acgtccttga ggcgagagat gtccagatgg tgcgtcgcca
     5401 gaaaacctcg caccgactcc tgcgcaccgc cgggcaatac caggaccgcc cccggataga
     5461 gcttccgggc ggccaccatc gaggccagtc catcgaagtc cgcattcaga tgtgtcgtga
     5521 tcagatccat gccggcattc taacaggatc gagcgaatcg attcagtccc gcagtggaga
     5581 cagaggagaa ggaagattgt gcacaaagcg agagcattgg ctggagagga accatgccgt
     5641 ccacacaaga agtgcggacg gcatggggtt actcgacaga atgagcgagc gcggatgcct
     5701 ggctgggtcg aaccggtcgc tcgaccgctc accacgacat ctgtgacacc gtctaccgct
     5761 gctggcggtc gtgcttctgg cgagcaatat gacgcgacgt gcgatcctgt gcgtggttga
     5821 tccgggcccg ctctttcttc gtcaccacgc cgtccgattt cgccttgtcc tccagcttgt
     5881 tgacgtgttc ttgctggttg ttcaagcgag tggcttcacg ctcgttcaac tgaccgctgg
     5941 caatcccctg atcgatccgt ttctcctgat tggcctgccg ctgatcgatc ctcggcgtct
     6001 ccgcctgcgc aaacaccaac gacgtggcac acaccgacaa caacgccgcg ccccacatca
     6061 cctgcttcat acatcctcct ttggttgccc cccgttgcac tgtcatccgt tccaacgtac
     6121 aaggacgccg gctggttgac aatcaaaatg ggggtttgaa gggaagtgcg gagaaccaat
     6181 gtcacggaca gaccacgcga gatggagatg aaatcagcga atgtcctgaa aaaaggcgcg
     6241 tgggagatac ggcagcttga actccacatg caaaatgacc agcaagacgc gaagaaaact
     6301 ccgccagatt cccattttga gaaacttgcg ggcatccgtg acgaccggtg gtgagagaag
     6361 cagcggcgtg gtgacttcca gcaaattctc accgaacgcc acatcttcca gaatgggccg
     6421 atccggaaat ccgccaagcc gctcgaacaa caccctccgc acgaataagg cttgatcgcc
     6481 gtaaatgata tggctcctga cacatcgaaa attgtcgagc catgagatca tccggagccg
     6541 ccagtccgac cctgagaatc ggtgtcggaa tccgccggcc tggatagtaa gattccgctc
     6601 caacgcattg atccgactca gcgcatcgac gggtaacagc gtatcggcat ggagaaagag
     6661 caaccactcg ccccgcgcca gcttcgcgcc ggcattcatc tgggacgctc gtccttttgg
     6721 cgccgtcacg agcgtgagcg gggccggcaa tcgactattg atcggccagc gccgaacgat
     6781 ttcgcaggtt cgatcgtggc ttcccccatc aaccacgatc acttcatacg acccggcttg
     6841 ttcgaacaac tgtcggagtg tggctggcag cgctcgctct tcattgtagg cgggaatgac
     6901 gacggagatc attccgtcaa ccacatgggc aggaaggtac gtacatcggc ggcccatgca
     6961 gacgcgtcct cgtcgatcgt ctcgaagaga tcgcagagcg tcaacaccat tgagtcggtc
     7021 aaagccagat agtcctgcgt gcgcaaacga agcgactcca actccctgtg cccatccacc
     7081 atcgctcgct ccaacacacg ccgcccgaag tcatgatgag cctcttcctg gtgcagcagc
     7141 actcggcgca accggcggaa cggcgcctgc cgcttttcaa ggccaccctc gattcgggtc
     7201 aaaatcgcct cccccagtcc ttccaggatc acctgttcag ccaggatact ctccagccaa
     7261 tcccctcgtt cgagcgcctc cgcgagaagg cggcgatagt cttcgagtgc cggcagaaag
     7321 ggcgcgtcac cgagatgacg cggtgcaagc cacgcaattg cgccgcggaa gaccacggca
     7381 tgcatcgcct cctgcctggc ttgggtcgtc aagaaacgac gcgccttcgg gtcgggagcc
     7441 agtgtggctt ggcgctgcgc acacccatgc gccattcgtt cgccatgttc
//
LOCUS       DAOQRM010000122         7323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_9877_length_7323_cov_15.726059, whole genome shotgun sequence.
ACCESSION   DAOQRM010000122 DAOQRM010000000
VERSION     DAOQRM010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9877_length_7323_cov_15.726059"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..145)
                     /locus_tag="PKK81_09785"
     CDS             complement(17..145)
                     /locus_tag="PKK81_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33212.1"
                     /translation="MHDPALWTSWYVWLLALPALVIGTLIWDWIQRRQPVRVRRRR"
     gene            complement(416..1330)
                     /locus_tag="PKK81_09790"
     CDS             complement(416..1330)
                     /locus_tag="PKK81_09790"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNV33213.1"
                     /translation="MPATLQAFIAIFLLGALLRSSGLLGRIHAERLAFVVFSISLPAT
                     ILVSLDRVVFAPTAWKLPLAACLVTLSMLFCSWPVARLLHLPRATRGGFLLGTGCINS
                     VYFAYPVVLATLGDKGLAQAILFDLGQTTLTLTVIYGLAVWHGARSATARSVMLRFVS
                     SPPLWALCLSLLLTLLERHLPGWLHDTLTPIHLTTTPLASLVLGLSISFSAVRRTWAL
                     ALLGVGMRMIGGLLLGFAVAYGLDLAGMERAIVVLVSAMPSAVTAVIFATDTGLDENL
                     VTSIVALSICVGVALLPWLPQLVFLLAR"
     gene            1429..2667
                     /locus_tag="PKK81_09795"
     CDS             1429..2667
                     /locus_tag="PKK81_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNV33214.1"
                     /translation="MAHPMTHPESSTQPRQAAVFFILVTVVLDMLSFGIIIPVLPKLV
                     EEFLGGDTAQAAEIYGLMGTSWALMQFVCSPIQGALSDRFGRRPVVLLSNLGLGLDFI
                     LMALAPSLAWLFAGRVISGIASSSFSTAGAYIADVTPPDKRAAAFGMMGASFGLGFVL
                     GPAVGGLLGAVDPRWPFWGAAATSLLNACYGFFVLPESLPIEKRAPFRWKRANPAGAL
                     MLLRSHHELFGLATANFLMNLAHGVLPSVAVLYLGYRYGWGPSAVGFTLAAVGVCAMI
                     VQGTLVRPITARLGERRTLITGLLCGAAGFAVYGLAPTPLIYCLGIPVMAFWGLAGPS
                     AQMFMTRRVSASEQGQLQGAIASLTGIAGLIGPTLFTQTFAAFIGPQADWHLPGAPYL
                     LSTTLLLIGAGIAWRATRAV"
     gene            complement(2672..3685)
                     /locus_tag="PKK81_09800"
     CDS             complement(2672..3685)
                     /locus_tag="PKK81_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33215.1"
                     /translation="MHHRSALHHRSKPGLIRHIVISCSLLLLWGLSGQCADQAFSAEP
                     AAPAASPNTLSGHELLRIGTIHDQQEHFPESLTYYNLALSKFREKRQPQGIATTLVKI
                     ARIDERQGHLQQAHTSLKEAVSLFAKASDRSAHAESLLAIGRVSAQLGLSDESRDALT
                     QAAALFTRVRNARGWNDTMVQLGLLQIAQGENSAGLTTLQQASEEARTRRHVDQQFTA
                     TVALGDAHWLLGHTSDAHSAYLDALSLAEAEHHLPFEGMMQLRLARLDGDQASLKDRV
                     AQGKRALQIAQAVHDTAAEAEAWSLLADLYGQLGQATESQDADTHALAIYRNRELSVH
                     GNR"
     gene            3808..4578
                     /locus_tag="PKK81_09805"
     CDS             3808..4578
                     /locus_tag="PKK81_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV33216.1"
                     /translation="MSTTRATPKRARISLDRSIERLRKQLADLAAYLGRGLPTRSLEE
                     FDQDTENLIAELLGDTSELLEAYEYAEFGEAAGLVNLTDEAPESAGVESQRQRLLQRS
                     RVLESCIAELEARRAAEPKQSQASRQTLIGPQVAEHMSSEIRSLSQDASLKDAGQAME
                     KWKLGSLLLTDKQAYVGFITDSDLARAVVARGVDPATGVNVYMRRPVVSIAGDRPIIE
                     AVRMMKDQATRHLAVTQDGSIIGVISVSNILRYYSGVV"
     gene            4739..5098
                     /locus_tag="PKK81_09810"
     CDS             4739..5098
                     /locus_tag="PKK81_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33217.1"
                     /translation="MGPTKAIVQGQSLHEAVSGKLIRDGFTSQQAMEDYVRQHYIVMP
                     VRDNAGQPWQLDGKPVYCLHGVQYETVDDQKVHLSRCPDCGGMGIRADELTVDSDCIR
                     CTACGHEFDARLEMMET"
     gene            5422..5997
                     /locus_tag="PKK81_09815"
     CDS             5422..5997
                     /locus_tag="PKK81_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV33218.1"
                     /translation="MAGATVTRPRKKAPQQNKKAPARKPKKARVQEPSGAVIVLSGAT
                     PLRRQKAAEVLAEELQLDLASVDLSAVVSKHAGETEKNVNRVFDMANRHGSILFFDEA
                     DALFGKRTQVHGAHDRYVNAEVSHLIQCIEDHQGLVILTSNGKSRIEEAFSPEKPVIV
                     LGGASKKKTATKPKPAAKSAPMPKKKVAKKA"
     gene            6021..6470
                     /locus_tag="PKK81_09820"
     CDS             6021..6470
                     /locus_tag="PKK81_09820"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNV33219.1"
                     /translation="MVLPLRSPREGLGGYVILPRLIDKVRLHALGALPSDYVRNLLKP
                     GLTLDGRFLKFTGLAADALQGAILSSPTDELVLAWVDQHAANHSDAEKQAWSEKAAAF
                     RPDPRVAKFLMASLPALAASLDITAYTLFDLIDMDEGRLPIPPNPAT"
     gene            complement(6508..6813)
                     /locus_tag="PKK81_09825"
     CDS             complement(6508..6813)
                     /locus_tag="PKK81_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNV33220.1"
                     /translation="MSQPGEGPSLGTETPASHHMIWIVYILECIDGSLYTGITNDLER
                     RMKAHATGKGAKYTKRRGPFTVRYIESLDSKGAALQREAAIKSLDRATKMALIASLP"
BASE COUNT         1422 a         2085 c         2396 g         1420 t
ORIGIN      
        1 cggtggaagt gcggcgttag cgccgtctcc gcactcgcac gggctgccgc cgctggatcc
       61 agtcccagat gagggtgccg atgaccagcg cgggaagcgc caacagccag acataccagc
      121 ttgtccagag cgcggggtcg tgcatgatcg ttcctccagt gtgtctggca gggaatagag
      181 caagcaccat accgtcgtgc attgttccga gggattgcgc cgcaaaccgt gaaacctccg
      241 gcgattcgca tagatgcaag gttatggcgg cgatgtttcc tgtcgcaaag cgcgccaggc
      301 aaattttgcc gtcgcataaa cctcacaata cgtgacgcca ttcccacagg tgccggatgg
      361 agacaacctg gggaggtcta gggagccggt gcatgggagc agggattcgg ctcgtttatc
      421 ttgccagcaa gaagacgagt tgcggcagcc agggcagcaa ggccacgccg acgcagatcg
      481 agagggcgac aatcgaggtg acgaggtttt catccaatcc cgtgtcggtt gcgaaaatga
      541 ccgccgtcac ggcggacggc atggcggaga cgaggacgac gatcgcccgt tccatgcctg
      601 caaggtcgag cccataggca accgcaaacc cgagcagcaa tccgccgatc atccgcatgc
      661 ccacgcccag caatgccagg gcccaggttc ggcgcacggc cgaaaaactg atcgacaggc
      721 ccagcaccag actcgccagg ggtgtcgtgg tgaggtggat gggggtgagt gtgtcgtgga
      781 gccagccggg aagatggcgt tcaagcaggg tgagcagcag gctgagacag agggcccaga
      841 gcggcggaga cgacacaaac cgcagcatga ctgatcgagc ggtggcgctc ctcgccccgt
      901 gccagaccgc cagcccataa atcaccgtga gtgtcagcgt cgtctgcccc aggtcgaaca
      961 ggatcgcctg cgccagtccc ttgtcaccga gggttgcgag gacgacggga taggcgaaat
     1021 agacggagtt gatacagccg gtgccgagga gaaatccccc tcgtgtggcg cggggtagat
     1081 gcagcaggcg ggcgaccggc caggagcaga acagcatcga gagggtcacc aggcaggcgg
     1141 ccaacggtaa cttccaggct gtgggggcaa agaccactcg atccagtgac accaatatcg
     1201 tggccggcag gctgatcgaa aagacgacaa aggccagacg ttccgcatgg attctcccga
     1261 gcaggccgga tgaccggagc agcgcaccga gcaggaagat ggcgatgaag gcttggaggg
     1321 tggcgggcat cgacgggagg gtagcagaaa acccgttcga agaggaggtg cgtcgtccga
     1381 ctgatccccg cgtctttttc tatgcgcctg aacggcgggc ctgctaccat ggcccatccc
     1441 atgacgcacc cggagagctc aacacaaccg cggcaagcgg cggtcttctt tatccttgtc
     1501 acggtcgtgc tcgacatgtt gtcgttcggg atcatcattc ccgtcctgcc gaaactggtc
     1561 gaagagtttc tcggcggcga taccgcgcag gccgcggaaa tctacgggct gatgggcacg
     1621 tcctgggcct tgatgcagtt cgtctgttcg ccgattcaag gcgcgctgtc cgatcgcttc
     1681 gggcggcgtc cggtcgtgct tctctccaac ctagggctcg gtctcgattt cattctcatg
     1741 gcgctggcgc ccagtctcgc ctggctcttt gcaggccggg tgatctccgg catcgcctca
     1801 tcgagtttca gcacggccgg tgcctacatc gccgatgtga cgccgccgga caaacgcgcg
     1861 gctgcattcg gcatgatggg ggcctcgttc ggtctcgggt ttgtgttggg tcctgccgtg
     1921 ggtgggctgc tgggcgccgt cgatccccgc tggccctttt ggggcgcggc ggcgacgagt
     1981 ctgctcaacg cctgttacgg gttctttgtg ctcccggaat cgctcccgat agagaagcgg
     2041 gcgccgttcc gctggaaacg ggccaatccg gccggcgcgt tgatgctgtt gcgctcacat
     2101 catgagctgt tcggtctggc gacggcgaac tttctgatga acctggccca cggggtgttg
     2161 cccagtgtag cggtgttgta cctgggctat cggtatggct gggggccgtc ggcggtgggg
     2221 ttcacgcttg cggcggtcgg ggtctgcgcc atgattgtgc agggcacgct ggtgaggccg
     2281 atcacggcgc ggctgggaga gcggcgcacg ttgatcaccg ggctgctgtg cggggccgcc
     2341 gggttcgccg tctatgggct tgcgcctaca ccgctcatct attgcttagg cattccggtg
     2401 atggcgtttt ggggactcgc cggtccgtcc gcgcaaatgt tcatgacgcg tcgcgtgagt
     2461 gcgtccgagc agggacaatt acaaggtgcc attgccagct tgaccggcat cgccggcttg
     2521 atcggcccca cgctcttcac gcagaccttc gccgcgttca tcggcccgca ggccgactgg
     2581 cacctgccgg gggcgcccta tctgttatcc acgacgctgt tgctgatcgg ggcaggtatc
     2641 gcctggcggg cgaccagagc cgtctgacgg gctatcggtt cccgtgcacg gacaactcgc
     2701 ggttgcggta gattgccagc gcatgggtgt ccgcatcctg agactccgtg gcttgcccca
     2761 gttgcccata gagatcggcc aggagcgacc aggcttccgc ctcggcggcg gtgtcgtgga
     2821 ccgcttgcgc aatctgcagg gcgcgtttcc cttgggcgac gcgatccttc agggaggcct
     2881 ggtctccatc cagccgcgcg agccgcagtt gcatcatgcc ttcgaacggc aggtgatgtt
     2941 ccgcttccgc caggctgagg gcgtcgagat aggcgctgtg cgcgtcactg gtgtggccga
     3001 gcagccaatg ggcatctccg agcgcgaccg tggcggtgaa ctgttggtcc acgtgccgtc
     3061 tcgtacgggc ctcttccgag gcctgctgca aggtggtcag ccccgcgctg ttctctccct
     3121 gtgcgatctg tagcaacccc aactgcacca tcgtgtcgtt ccatccccgg gcatttctca
     3181 cgcgcgtgaa gagggcggcg gcctgggtga gggcgtcgcg cgattcgtcc gatagcccca
     3241 actgcgccga gactcgaccg attgcgagca gcgactcggc atgggcggat cgatccgacg
     3301 ccttggcgaa cagcgacacc gcttccttga gtgaggtgtg ggcctgctgc aggtgaccct
     3361 ggcgttcatc gatccgcgca atcttcacca gcgtggtggc gatgccttgg ggctgtctct
     3421 tctcccggaa tttcgacaag gccaggttgt aatacgtcag cgactccggg aaatgttcct
     3481 gctgatcatg gattgtcccg atgcgaagca gttcatggcc tgacagggta ttgggagatg
     3541 cggccggtgc ggccggttct gcggagaacg cctggtccgc acattggccg gacaggcccc
     3601 agagcaggag aagcgagcag ctgatcacga tgtggcgaat caggcctggt ttcgaacggt
     3661 gatggagtgc tgaccggtga tgcatggcct aaacatgcac gtgccggttg ccaaggtcaa
     3721 gtcggtgcgg ttgcatgggc ccggcgcttg cctcgcctgt tccggagggg taagatcggt
     3781 catcccgcca tacagagagg aggtgccatg tctacgacac gcgcgactcc caaacgagcc
     3841 cggatctccc tcgaccgcag catcgagcgt ttacgcaagc agttggccga tctggccgcc
     3901 tatcttggca ggggccttcc cacccgcagc ctggaagagt tcgatcagga caccgaaaac
     3961 ctcattgccg aattgctcgg ggatacctcc gagctgcttg aggcgtatga atatgccgag
     4021 ttcggcgaag cggccggctt ggtgaatctg acagatgaag caccggaaag cgccggtgtg
     4081 gagagtcagc gacagcgcct cttgcaacgc agtcgggtgc tggaaagttg cattgccgaa
     4141 ctcgaagccc gacgcgctgc cgaaccgaaa cagtcacaag ccagccggca gaccctcatc
     4201 ggtcctcagg tcgccgagca tatgtcgtcc gagatccgta gcctgtccca ggatgccagc
     4261 ctcaaggacg ctggtcaggc catggagaaa tggaaactgg gctcgctgtt gctgaccgac
     4321 aagcaggcct atgtcgggtt catcaccgat tcagacctcg cccgcgcagt ggtcgccagg
     4381 ggggtggatc ctgcgacggg ggtcaacgtg tatatgcgaa ggccggtggt gtcgatcgcc
     4441 ggcgaccgtc cgatcatcga ggcggtgcgc atgatgaagg atcaggccac gcggcatctg
     4501 gcggtcacgc aggatggctc gattatcggc gtgatttctg tgtccaacat tctgcggtac
     4561 tactcgggcg tggtgtagcc ggacagtgtg accatcacgg gatggtcaaa aaggctgtca
     4621 gcaaggccgc agcgagtgag gggccgaggc gtaccctcaa gggtacgttg agggtccgag
     4681 cgatgcgaga acgcagctgg cgggcttttt caacatccca ggcaaaagga ggcgggatat
     4741 gggcccgacg aaagcaatcg ttcaaggaca gtcgctccac gaagccgtga gcggcaaact
     4801 gatacgggac ggatttacga gccagcaggc gatggaagat tacgtgcggc agcactatat
     4861 cgtcatgccg gtacgcgaca atgccggaca gccctggcag ttggacggga aaccggtcta
     4921 ctgtctgcac ggtgtgcaat acgaaacggt cgatgaccag aaggtgcatc tttcccgatg
     4981 cccggattgc ggcggcatgg ggatcagggc ggatgaactc acggtcgatt ccgactgtat
     5041 tcgctgcacc gcctgtggcc atgagtttga tgcgcggttg gagatgatgg agacatagga
     5101 gaggggcctg cctgctcagt gtcttttgct cgcgcaacgc gcggccttag aaggccctcg
     5161 ttggacgcgc gcagtgagac accgagcagg caggccccag acagaaaaga gagggctatc
     5221 agcgatacgc tcttattccc cgcgagatac gtttcacgtc ttacgtgtga cgcttcacgc
     5281 tcgacgcttc gcgattgatc tgccttccgg aggagtaaga agaaattcag cgacaggaat
     5341 gagaaccggt tatactcagc gcctgctcct cataagagga ggccacgtgg gtcatactag
     5401 aatcggaaag ggacgaagga catggcaggt gcaacggtga ctcggccacg caagaaggct
     5461 ccgcagcaga acaagaaggc gcctgcgaga aaacccaaga aggctcgtgt gcaggagccg
     5521 tccggtgcgg tgatcgtgct gtccggagcc acgccgcttc gccggcagaa ggccgcggag
     5581 gtattggccg aagaactgca attggacctg gccagcgtgg atttgtcggc ggtggtgagc
     5641 aaacacgccg gcgaaactga aaaaaatgtc aatcgcgtgt tcgacatggc aaaccgccat
     5701 gggtcgattc tttttttcga tgaggccgac gcgctctttg gcaagcgcac ccaggtgcat
     5761 ggcgcgcacg atcggtacgt aaatgccgag gtatcccatc tgattcagtg catcgaagac
     5821 caccagggcc ttgtcatcct gacgagtaac ggaaagagcc gcattgaaga agccttctcg
     5881 cccgagaagc cggtgattgt gctgggcgga gcctcaaaga aaaagaccgc aaccaagcca
     5941 aagccggcgg ctaagtccgc acccatgccc aagaagaagg tggcaaagaa ggcgtgagca
     6001 ggggcacggt gtctgcaggg atggtactcc cattgcgatc accgcgtgag gggcttgggg
     6061 gttacgtcat cctccctcgg cttatcgata aggtgcggct ccatgcgttg ggcgcgttgc
     6121 catcggacta tgtgcggaat ttattgaagc cagggcttac ccttgatggc aggttcctga
     6181 aattcaccgg actcgctgcc gacgcgctgc aaggcgcaat attgtcatcg ccaacagacg
     6241 aactcgtctt ggcgtgggtc gatcaacatg cggcaaacca ttcagatgcc gagaaacagg
     6301 catggtcaga gaaggcggct gcttttcgtc cggatcctcg cgtggctaaa tttttgatgg
     6361 cgagcctccc tgctctcgca gccagcttgg atatcactgc ttataccctc tttgatttga
     6421 ttgatatgga cgaagggcgc ctaccgattc cgccgaatcc ggcgacctga agtgggcgga
     6481 ccggtcagag ttggccgcct cgcttcatca cggtagggat gcaatgaggg ccattttcgt
     6541 ggcccgatcc agtgacttga tggcggcttc gcgttgtaag gcggctccct tgctgtcgag
     6601 tgattcgata taccggaccg tgaacggtcc gcggcgtttc gtgtattttg cccccttccc
     6661 ggtcgcatgc gccttcatgc gccgttccaa atcattcgtg atcccggtgt agagactgcc
     6721 gtcgatacac tccaggatgt agacaatcca gatcatgtgg tgtgacgccg gggtctccgt
     6781 gcccagggaa ggtccttcgc ctggctggct catgatagcg aaaaagatcc ggcgtggcga
     6841 ctatttgggg aaagcgtggg ggaagtcgag caccagtctt gaaaggatgg ccgccaagac
     6901 gagggctgag gccagtgcca cgatcaggaa cttgcggtct ttgcgcaccg aatcgtggaa
     6961 tcgatatctg ctggatctgt gtagagactg acttgccacg cggccgtacc tctggagtct
     7021 atgaaatggc ccgtgcacta tgcctggctg atgcataggc tggaataggg gttttcctga
     7081 aaggctctgg aattattact gtctcgaagt tattcggtcc tgttcactgt tctgcatact
     7141 cgtccgatga gtccccggta tcgagctgag ttcctgccga tgtggccctg attgacagct
     7201 cagtcggcgg ccacgtagcg tacccattca tgtctgaggc aacgatgatc gatcgagaac
     7261 tcgccgccgc tgaagttgcg tggacacaag gcaatgcagg caaggcgcgg gtctgcgcgc
     7321 ggc
//
LOCUS       DAOQRM010000123         7181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10172_length_7181_cov_12.589391, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000123 DAOQRM010000000
VERSION     DAOQRM010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10172_length_7181_cov_12.589391"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..411)
                     /locus_tag="PKK81_09830"
     CDS             complement(67..411)
                     /locus_tag="PKK81_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV33221.1"
                     /translation="MPIYEYRCRQCGKRTTRLILSISAAPQQTCGHCQSPDLERLLSR
                     FASPKSEEARLEALSDPSHLSGFDENDPQSMARLMKKMGQEMGEDLGDDVEAAMEGGG
                     AAPPGTDQTDFE"
     gene            complement(414..1226)
                     /locus_tag="PKK81_09835"
     CDS             complement(414..1226)
                     /locus_tag="PKK81_09835"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNV33222.1"
                     /translation="MSSHALFAGILRQATRLVLPSDCTTCEQPLTDDPTPFFCRQCWE
                     LIRPLQGPSCPRCHRPFASPFATTHSPTHECHDCRTRTPHYSQVWAAYAYCAPLQEAI
                     ALFKYRGKVVLADALGALLARALPADLNADLLMPIPLHPDRLRQREFNQSLLLADRVG
                     PVLRRPVSYRNLVRIVDTAPQTTLPRSARLQNLHQAFALRAPGEVTDKRVLLIDDVFT
                     TGTTANECAKVLLKAGAEDVAVLTLARSVDAGMVPETWLPPSAFDQPHALRV"
     gene            1428..2126
                     /locus_tag="PKK81_09840"
     CDS             1428..2126
                     /locus_tag="PKK81_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV33223.1"
                     /translation="MQDPTNMLVRVQNLSKEYRRGQTLVSALQDVTLDVKAGEFCAFV
                     GPSGCGKSTLLNLIGGLDQPTAGTIAIENRPVTHASATEWTRLRRELIGIVFQAFHLI
                     PGLTVAENVALPLLLKGERGAAVAERVNEVLEAVRMDRRRSHRPGELSGGEQQRVAIA
                     RAIVHRPRLVLADEPTGNLDSKQGADIVSLFRTLPQRFGHSVLLVTHSDSAADAADYV
                     WHMRDGRLVNCVRH"
     gene            2270..3196
                     /locus_tag="PKK81_09845"
     CDS             2270..3196
                     /locus_tag="PKK81_09845"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HNV33224.1"
                     /translation="MDLSVTIAGVKFPTCFMNASGALCVTREELITLGRSRAGAIVTK
                     SMTLEPRVGNPEPRYYGFPGGSINSMGLPNLGYRAYAEMIPELTRFGKPVIASIAGLC
                     EDDFLTMARVINQARPDLIEVNLSCPNIPGKPQIAYDPVDSERLLKRVRPLITVPMGV
                     KLPPYFDPAHHAVMADVIRRCGVDYLNLINSVGNGLVVDPKRETPVIKPKGGFGGLGG
                     SLIKPVALANVRAFWKLLEGRIPIIGTGGVVQGVDAFEHVLCGASAVQVGTALVEEGV
                     GVFERLERELAAELAMRGKQSLEECRGKLKEL"
     gene            complement(3247..3477)
                     /locus_tag="PKK81_09850"
     CDS             complement(3247..3477)
                     /locus_tag="PKK81_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV33225.1"
                     /translation="MRAKRSEGLTPRQKEILKLVSQGNTNRDIARRLDISVRTVEVHR
                     FNLMRRLKVRNVAQLLRQALQQGFLPKNFAFR"
     gene            3681..>4030
                     /locus_tag="PKK81_09855"
     CDS             3681..>4030
                     /locus_tag="PKK81_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33226.1"
                     /translation="MPLISSALFEVCARLGWTHLSTHPGRTLLTIIGVGLGVAATIAV
                     QTANVDVLRSFEDSVLAVAGPVTLEVSAGEAGMDERLIASVRGIAGVDSAKPVGELGV
                     RVAGRSQSFLILGL"
     assembly_gap    4031..4130
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4131..6149
                     /locus_tag="PKK81_09860"
     CDS             <4131..6149
                     /locus_tag="PKK81_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNV33227.1"
                     /translation="FQRTFGLSGRLDRIDVVTQASASVEQVAEAIARILPPAVTVRRP
                     IQRSQQVESMVGAFQLNLSVLSMVGLLVGMFLIYNTVSFTVAQRRREVGILRAIGMSE
                     PMVVGLFLAEAGVFGVAGGVLGGSLGLVLGNVLVGLVGRTIQDLYVPMADAPRTFGFP
                     PGSVRLLTEAIVIGSGVSILGALGPSLDAGRTMVVAALAPGEYDVAQRVRAASLGVVG
                     GGLLLFALGCALAGPVGGVPVFGYLATFCLLAGLSCLVPILMQWVCRTRELGAASASP
                     SLGGAIRHIAREQTTRGIGRNAVTVSAFLVGVAIMVGVMVMIRSFRNTVEMWIDQTVM
                     ADFIVAPAGWPHVVRGGSSSQALPGSWRTRLAGATHVSAVDAYRDVRIELEGRPIALV
                     SRDLALHAARSRYLFLEGDSAAILTRAAAGEGAILSEVVANHLHVIRGSQLSISTPVG
                     EKSLPVLGVFYDYATDGGKIVIDRSLYQHWWNDDGVTVFPVYIDPEVDLEQARAALLA
                     TLADGSRGNLLPTVLSNGELRREILRIFDRTFTLTYVLEAIAIIIAMLGIINTLVTSV
                     VERRRELATLQALGSSRGQITALILWEAGYLGLLGTAMGLVGGLALAWILIRVINRQS
                     FGWTIQVSWPLGLMAEVAVLALVASLLAGFWPARWAGRQPLVEGLRYE"
     gene            complement(6140..>7181)
                     /gene="bioB"
                     /locus_tag="PKK81_09865"
     CDS             complement(6140..>7181)
                     /gene="bioB"
                     /locus_tag="PKK81_09865"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNV33228.1"
                     /translation="VACMTDFTILADKALRDEILTHEESLAVLQTPDSRLLELLQAAF
                     TVRERYFGKTVRLQMLLNAKSGACQEDCGYCSQSAISKAPIERYGLLTQDQMVTGARR
                     AAAAKAQRYCIVISGRSPIDRDIADIASAVRSIKQEVPIQICCSLGLLNERQAKDLKA
                     AGVDRINHNLNTSEAYHPEICTTHTFQDRLATIRHARTAGLEICSGGIVGMGESDEDL
                     IDLALALREVKPDSIPINTLHPASGTPMEHCAPLTPQRCLKALCLFRLLHPRTEIRIA
                     GGREHNLRSLQPLALYPADSVFVNGYLTTPGQPAAEVWRMIEDLGFEIQVDAVPTKQG
                     IPASEPVAGLHS"
BASE COUNT         1216 a         1875 c         2432 g         1558 t
ORIGIN      
        1 ccatttcttc gctgatggta ggagacaagg ccaatcctgt caagtgaata ttcatgaccg
       61 gggcggtcac tcgaagtcgg tctggtccgt tccgggtgga gcagcgcccc cgccttccat
      121 cgccgcttcg acgtcatccc cgaggtcttc tcccatttcc tgccccattt ttttcatgag
      181 ccgggccata ctctgcgggt cgttttcgtc aaatccgctc agatggcttg gatcggacag
      241 ggcctcaagg cgtgcttctt ccgatttggg agaggcaaac cgggagagca gtcgttccag
      301 atccgggctc tgacagtggc cgcatgtctg ttggggcgca gcgctgatgc tgaggatcag
      361 gcgggtagtt cgtttcccac attggcgaca gcgatattcg tagatcggca tagttagacc
      421 ctcaaggcgt gcggttgatc gaaggccgat ggcgggagcc aggtctccgg caccatgccg
      481 gcatccacgg aacgcgcgag cgtgagcact gcaacgtctt cggcaccggc tttgagcagg
      541 actttggcgc attcgttggc tgtggtgccc gtggtgaaga catcgtcgat gaggaggacg
      601 cgcttgtcgg tcacttcccc tggggcgcgc agggcgaacg cctggtgaag gttctgcagg
      661 cgtgcggagc gggggagcgt ggtctggggg gcggtatcga cgatgcgaac caggtttcga
      721 taggatacgg gtctcctcag tacaggtccg acgcggtctg cgagcaagag tgattgatta
      781 aattctcgct gccgaaggcg gtcggggtgg agcgggatcg gcatgaggag atccgcattc
      841 agatctgcgg gaagagcccg ggcgagcaac gcacccagcg catccgccag caccaccttt
      901 ccgcgatatt tgaagagggc aatggcctcc tggagcggtg cgcagtaggc ataggccgcc
      961 cacacctgcg aatagtgcgg tgtgcgggta cggcagtcat ggcattcgtg agtcgggcta
     1021 tgggttgtcg cgaaagggga ggcgaaaggg cggtggcatc gcgggcagga gggcccttgt
     1081 agcggtcgaa tcagttccca gcattggcgg cagaagaacg gcgtgggatc gtccgtgagg
     1141 ggctgttcac aggtcgtgca gtccgacggg agtacgagtc gtgtggcttg cctgaggatg
     1201 cctgcgaaca gtgcgtgtga gctcattggc taggtccaag aggcgtgacg gacgcgcgtt
     1261 gggattgtgt acggaagacc cctcagccgt caaggttgtg gggtacaaac cgggtgtggt
     1321 ataggaccac tcgcctgcat cggagcgatt ccagaggctc ggcaatgtgc cggttgcatg
     1381 catctgccgg atgcttccgc actccgtcag ggctatcgat tcccatcatg caagatccca
     1441 ccaacatgct ggtacgggtg cagaatcttt cgaaagaata tcgccgcggg cagacgcttg
     1501 tgtcggcgtt gcaggacgtg accctcgacg tgaaggcggg agagttctgt gccttcgtcg
     1561 ggccgagcgg gtgcgggaag agcacgttgc tgaatctgat cggagggttg gatcagccca
     1621 cggcgggaac catcgccatt gagaaccggc ctgtgaccca tgcgagcgcc accgaatgga
     1681 cccgcttgcg acgcgagttg atcggaattg tctttcaggc cttccacctt attcccggcc
     1741 tgacggttgc ggagaatgtc gccttgccgt tgctgctgaa gggggagcgg ggggctgcgg
     1801 tggctgagcg ggtcaacgaa gtcttggaag cggtgcggat ggacaggcga cgttcgcacc
     1861 ggcccggcga gttgtcggga ggggaacagc agcgtgtcgc gattgcccgg gcgatcgtgc
     1921 atcggccacg acttgtgctg gcggatgaac cgaccgggaa tctcgattcc aagcaagggg
     1981 cggacatcgt cagcctgttc cgtacgttac cgcaacggtt tggtcactcg gtgttgctgg
     2041 tgacgcacag cgatagtgcg gccgacgcag ccgactatgt gtggcatatg cgggatggcc
     2101 ggctggtgaa ttgtgtccgg cactaggccg aacggttgtg tactatgttc gtgaaggcgg
     2161 ctcgtcgctc ggagagaggc gtcatgaata atgcgtcgcg aatttcaaag aggatggggg
     2221 aggaacaacg gcggggttgt tccatgtcct gtcgggagaa aggatcagca tggatttgag
     2281 cgtcactatt gcgggagtaa aattcccgac ctgtttcatg aatgcctccg gagcgctctg
     2341 tgtgacgcgg gaggaactca ttacgctggg gcgttcccgg gccggtgcca tcgtgactaa
     2401 atcgatgacc ctggagccac gggtcggtaa tcctgaaccg cggtattacg gatttccggg
     2461 ggggtccatc aattccatgg ggctgccgaa tctcgggtat cgagcctacg cggagatgat
     2521 ccccgaactc acccggttcg ggaaacccgt gatcgccagc attgccggcc tgtgcgagga
     2581 cgactttctc accatggcgc gtgtgatcaa tcaggcgcga cccgatctca tcgaggtcaa
     2641 tctctcctgc ccgaacattc ccggtaagcc gcagatcgcc tatgatccgg tcgattcgga
     2701 acggttgctc aagcgggtgc gaccgctcat caccgttcca atgggcgtga aattgccgcc
     2761 ctatttcgat ccggcgcacc acgcggtgat ggcggacgtg attcgacgct gcggggtcga
     2821 ttacctcaat ttgatcaact ctgtggggaa cggtctggtt gtggacccga agcgcgaaac
     2881 gccggtcatc aagcccaagg gcggattcgg agggttgggg ggatcgttga ttaagccggt
     2941 cgcgctggcc aatgtgcgag cgttctggaa gttgttggag ggacgtatcc ccatcatcgg
     3001 gaccggtgga gtcgtgcagg gagtcgatgc ctttgagcat gtgctgtgcg gtgcgtcggc
     3061 cgtccaggtg gggacggcgc tggtcgaaga aggtgtgggg gtgtttgagc gattggagcg
     3121 cgagctggcg gctgagttgg ccatgcgggg caagcagtcg cttgaggaat gtcggggaaa
     3181 actgaaagag ctgtaaagct gtaaaaaggt gcgccggttc cgtatatgaa accggcgcat
     3241 agagtgttat cggaaggcga agttcttcgg aaggaacccc tgttgcaacg cctgacgaag
     3301 cagctgcgcg acgttgcgga ctttcaagcg ccgcatcaaa ttgaagcgat gcacctcaac
     3361 ggtgcgcacg ctgatatcca ggcggcgggc gatgtcgcgg ttggtatttc cttgggacac
     3421 cagtttcaga atttctttct gcctgggcgt cagtccctcg gatcgtttcg cgcgcacggg
     3481 cgccggcttt acgacccttg caggttttct cttcttggtc ggtgaagcgg ccttggtggt
     3541 cttcgctggc tttctggctt gaggtcgttt gcgtagtgcc atatagatat tgtaccccaa
     3601 attctactga acgtaatctt agcatatctg ggtaaatctg taaaaacaat tactagagtg
     3661 ttgttccggt aggtctgccg atgccgctga tttcctctgc cctattcgag gtctgtgccc
     3721 gtcttggatg gacacatttg tccactcatc ccggtcgcac gctccttacg atcatcggcg
     3781 tcgggttggg ggttgcggcg acgattgcgg tccagactgc caacgtggac gtcctccgat
     3841 cgttcgagga ttcggtctta gccgtggccg gtccggtcac actggaggta tcggccggag
     3901 aagcgggcat ggacgagcgc ctgattgctt ccgtgagagg tatcgccggt gtggattcag
     3961 ctaaaccggt gggagagctc ggggttcgag tggccggccg ttctcaatcg ttcctcatcc
     4021 ttggactgga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttcaacgga
     4141 ccttcggttt gagcggccgg ttggatcgaa tcgacgtggt gacgcaggcc tccgcgtcgg
     4201 tggaacaggt ggccgaggcg atcgcaagga ttctgcctcc ggctgtcaca gtgcgccgtc
     4261 cgatccaacg cagccagcaa gtcgagtcga tggtgggcgc attccaattg aatctctccg
     4321 tgctgagtat ggtggggcta ctagtcggga tgttcctgat ctacaacacc gtgtccttca
     4381 ctgtggcgca acgccggcga gaggtgggaa tcctgcgggc gatcgggatg tcggagccta
     4441 tggtcgtggg tctgttcctc gcggaggcgg gagtgttcgg tgtggccgga ggggtcctgg
     4501 ggggtagtct gggcctggtt ctggggaatg tactcgtcgg gttggtcggt cggaccatcc
     4561 aggacctcta tgtcccgatg gctgatgcgc caagaacctt cggctttccg ccgggatctg
     4621 tccggctgtt aaccgaagcg attgtgatcg gtagcggggt gtcaatcctg ggagcgctgg
     4681 ggcccagctt ggatgccgga cgtaccatgg tggtcgcggc ccttgctcct ggggaatatg
     4741 atgttgccca gcgagttcgt gctgcctcac tgggggtggt gggtggaggc ctgctgctct
     4801 tcgcgctggg atgcgcgcta gccggaccag tcggcggggt tccggtcttc gggtatctgg
     4861 cgacgttctg tctgcttgcc ggtctctcct gcctggttcc gatcctcatg cagtgggttt
     4921 gccgtacccg agagttgggc gccgcttcgg cttcgccttc gttggggggc gccattcgcc
     4981 acattgcgcg ggagcagacg acgcggggaa tcggccggaa tgcagtgacc gtctctgcct
     5041 ttctcgtcgg ggtcgcgatt atggtcgggg tcatggtgat gattcggagt tttcgcaaca
     5101 cggtcgagat gtggatcgat cagacggtca tggccgattt catcgtggct ccggccgggt
     5161 ggccgcatgt ggttcgcggt gggtcgtcga gccaggccct ccctggttcc tggcgaaccc
     5221 gtctggccgg ggccacgcat gtgtcggcgg tcgatgcgta ccgcgatgta cgcatcgaac
     5281 tggaggggcg gccgatcgcg ttggtatcca gagatctggc gctccatgcg gcacgaagcc
     5341 gatatctatt tctcgagggt gactcggcgg caatcctgac tcgtgccgca gcaggagaag
     5401 gggctatcct ctcggaggtg gtggccaatc acctgcatgt gatcaggggc agtcagttgt
     5461 cgatttcgac gccggtgggg gaaaaatcgt tgccggttct gggggtcttt tacgactacg
     5521 cgaccgatgg cgggaagatc gtcatcgacc ggtctctgta ccagcactgg tggaacgatg
     5581 acggcgtgac ggtgtttcct gtgtatatcg atccggaagt cgatctggaa caggccagag
     5641 ccgccctgct tgcaaccttg gccgatggct cccgcgggaa cctgctgccg acggtgttaa
     5701 gcaacggaga attgcgccgg gagatccttc gaattttcga ccggaccttc acattgacct
     5761 atgtgttgga ggccatcgcg atcattatcg ccatgctggg gatcatcaac accttggtga
     5821 cgtcggtggt cgagcggcgt cgggagttgg ccacgttgca ggcgctgggc agcagccggg
     5881 gacagattac ggctctgatt ctctgggaag ccggctatct gggcctgttg ggaacggcga
     5941 tgggactcgt tggggggctt gcgctcgcct ggattttaat ccgggtcatc aaccggcagt
     6001 cgttcggctg gacgatccag gtgtcatggc ccctggggtt gatggcggaa gtggcggtgc
     6061 tggctctggt cgcttcgctc ctcgccgggt tctggccggc ccgttgggcc ggccggcaac
     6121 cgctggttga ggggctccgt tacgaatgaa ggcccgcgac tggctctgac gcaggaattc
     6181 cctgcttggt cggcactgcg tccacctgga tctcgaaacc caggtcttcg atcattcgcc
     6241 acacctctgc cgccggttga cccggcgtgg tgaggtatcc gtttacgaac actgaatccg
     6301 ccggatagag cgcgagcggt tgcaagctgc gcaggttgtg ttcacggccc ccggcgatac
     6361 ggatctccgt tcgcgggtgc aggagccgga agaggcacag ggccttcagg catcgttgcg
     6421 gcgtcagggg ggcgcaatgc tccattggag taccggatgc cgggtgaagc gtgttgatgg
     6481 gaatcgagtc cggtttgacc tcacggaggg ccagggcgag atcgatcagg tcttcatcgc
     6541 tctctcccat tccaacgata ccaccggaac agatttcgag tccggccgtc cgagcgtgcc
     6601 ggatggtggc cagccggtcc tgaaaggtgt gtgtcgtgca gatctcgggg tgataggctt
     6661 cgctcgtgtt caggttgtga ttgattcggt ccaccccggc ggctttcagg tctttcgcct
     6721 ggcgttcatt gaggagcccc agcgagcaac aaatctgaat cggaacttcc tgcttgatgg
     6781 agcgaacggc ggaggcgata tcggcaatgt cccgatcgat cgggctgcgt ccgctgatga
     6841 cgatacaata tcgctgggct tttgcagccg ccgcgcggcg ggcgccggtg accatctggt
     6901 cttgggtgag cagtccgtag cgttcgatcg gtgctttgga gatggccgat tgcgaacagt
     6961 agccgcagtc ttcctggcaa gcgccgctct tggcgttcaa gagcatttgc aggcgaaccg
     7021 tcttgccgaa gtagcgttca cggacggtga acgccgcctg caagagttcc agcagtcggg
     7081 agtccggtgt ctgcaatacg gcgagggatt cctcgtgggt gagtatttcg tcccgcaagg
     7141 ccttgtcggc caggatcgtg aagtcagtca tgcacgctac t
//
LOCUS       DAOQRM010000124         7106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10329_length_7106_cov_18.652673, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000124 DAOQRM010000000
VERSION     DAOQRM010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10329_length_7106_cov_18.652673"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKK81_09870"
     CDS             complement(<1..245)
                     /locus_tag="PKK81_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HNV33229.1"
                     /translation="MSKRDHILVGLDIGTTKICAIVAEVPEEGPLNIIGVGSCPSRGL
                     RKGVVVNIESTVESIKKAVEEAELMAAVQINSVYTGIA"
     gene            complement(251..1120)
                     /locus_tag="PKK81_09875"
     CDS             complement(251..1120)
                     /locus_tag="PKK81_09875"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNV33230.1"
                     /translation="MSLRWRKVRPVKVNPRANARSESALGRHRAGETGGYWSRLGRGL
                     LITLRVVATVTVLVGGCSGLFVLAREVGPLTREWFLVRSVSVSGLHHVTRKEVIGRLA
                     LKPDTALYSINPSWLADRIKTHPWIKDATVVLKPLHEIHIDIVEREPAVVVRTLAENL
                     LADSDGFLLAHLGSADDPTLPMLSGVDGKRLVQGKPDDRRPVQVGAALARMVGQTTGG
                     RPDINVGNLNNLVVEVQGVTFQFSESSMNQQWYRFLKMRPALRDVAFDGEGARANEID
                     LRFADRVIVRGRG"
     gene            complement(1123..2106)
                     /locus_tag="PKK81_09880"
     CDS             complement(1123..2106)
                     /locus_tag="PKK81_09880"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNV33231.1"
                     /translation="MTERKPLTRSKIGVLMGGQSSEREVSLKTGEAVYRSLVRSGYDA
                     VAIDVGPDLSKTLQEQAVEVGFLALHGPGGEDGAIQGFLETLGIPYTGSGVRASAIGM
                     HKVVTKTELAAQGIPVPRGTVVLRGTAPSLNRVLSTCKLKLPVVVKPASQGSTIGVTI
                     VRKPSQWKEALRTAHRYDEEAMVEAFIQGHEVTVSLLGGPDGVVTGLPAVEIVAPDGF
                     YDFSAKYEKGRTQYLCPAPLPAAVTRQIKQLAIRTYQALGCSGAARVDFRITPKGKPF
                     VLEINTVPGMTETSLLPMAAGKAGLSYDALTERILQSALGRAGVGSFRAVR"
     gene            complement(2131..3078)
                     /gene="murB"
                     /locus_tag="PKK81_09885"
     CDS             complement(2131..3078)
                     /gene="murB"
                     /locus_tag="PKK81_09885"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNV33232.1"
                     /translation="MRRTTVSRVAGKDHAARTRKDWARILEGVRGTITYEASLQAYTS
                     FHIGGPAEVLVEPADVEDLCRVVAQARAEHIPVFVVGGTNLLVRDGGIRGIVVSLRQF
                     KAIRQEPDHVLYAEGGVGMPTLIGYAIRRSLAGLEWGAGIPGTVAGCVVMNAGTRLGE
                     MKESVKAVRMVDPRGRVVDIPAADIPFSYRRAHLPRGIVAGVWVQLRPGDHDRIEKTV
                     KEYLQYRKNTQPLTLPSAGCVFKNPPQDSAGRLVDAAGLKGARIGDAQVSEKHANFMV
                     NVGHARAADVLALIRKVRAAVKKVSGVKLELELKVVGEA"
     gene            complement(3085..4467)
                     /gene="murC"
                     /locus_tag="PKK81_09890"
     CDS             complement(3085..4467)
                     /gene="murC"
                     /locus_tag="PKK81_09890"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNV33233.1"
                     /translation="MFRKTQHIHLVGIGGSGMSGIAEVLLTLGYKVSGSDLSQSETTR
                     RLEELGGRIAIGHHESNIGDAQVVVISSAVAATNPEVVAAKARQIPVIPRAEMLAELM
                     RLKFGVAIAGAHGKTTTTSMVANVLAQGGLDPTMVIGGKVNALGSHARLGRGDLLIAE
                     ADESDGSFLRLAPTIAAVTNIDREHLDHYGTMERLNDSFLEFVNKVPFYGLAVLCSDD
                     ERLRNLLPRVVKRYQTYGLHEHPGHVPDFRATDISLRQWGSEFRVFFRGRNLGPFRLS
                     IPGIHNVSNSLVAIAIGMELDIPVDLIRKGLAAFSGVERRFHLRGEKAGIMVVDDYGH
                     HPTEVRATIAAAKQGWDRRVVVLFQPHRYSRSRDLMQEFAHAFDQADALFMTEIYAAG
                     EQPIPGVSGAKLVEAVQAAGHPSATFVERKDTLAEQVLPTLKSGDLVITLGAGDIWKT
                     GLAILERLPA"
     gene            complement(4550..5671)
                     /gene="murG"
                     /locus_tag="PKK81_09895"
     CDS             complement(4550..5671)
                     /gene="murG"
                     /locus_tag="PKK81_09895"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNV33234.1"
                     /translation="MNVVIAAGGTGGHLYPAIAVAREFLKRDPSTRILFVGTTRGIER
                     KVLAHEGFPLQCIDANPLMGKSLLEILKALITLPKSLWQSLGILKKQSADLVFGVGGY
                     TSPAMLLAAFLRRIPGVILEPNAYPGMANKAVAPFVQRVFLAFESTVTFFDRCKTSVV
                     GTPVRQAFVESAAPDAEQAEARTARHLLIFGGSQGAKAINSAVIEALPLLRSMKDQLT
                     ITHQTGEADHARVVAAYEQAGMPAQVVPFLYDMPTVLRGADLVVARAGAMTIAELTVC
                     GKAAILIPLPTAIYNHQLRNAEVMAKAGGAVLLPQAELTGPGLAQTVMQILKEPHRLQ
                     TMSEQSWNMRRSDAAETIVRECYDVIRRRHEASDSARAL"
     gene            complement(5668..6876)
                     /gene="ftsW"
                     /locus_tag="PKK81_09900"
     CDS             complement(5668..6876)
                     /gene="ftsW"
                     /locus_tag="PKK81_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNV33235.1"
                     /translation="MAQHALGTLMLPWSTSTQRATKRVPVDPALLAVTLILALVGVVM
                     VFSASAVVAGNRFHDPWYFLKRQLAWLGAGLLVMHLISKIDYTIWKKLAIPLLFGTTV
                     LLVLVLVPSFGSVAKGARRWLHLGPLNIQPAELAKYAVAIYIAAYLTKKQDQITNFSR
                     GLLPPLIVLGLLSGLVLLEPDLGTVVVMGLVVVAMLFLAGARIKHLGLLALCALPTVA
                     ALILGSPYRRRRVIEYLYGAKDPTGSGYQIHQSFLAFGSGGPFGVGLGEGKQKLFFLP
                     EAHTDFVLALVGEELGLMGTVTIVLLFGLFVIKGFQIARRARNPFGRHLAMGITLLVG
                     MQALVNAGVVTGLLPTKGLTLPFVSYGGSSLMANLFGVGILLSISRDRQGGQDSSGPR
                     VVRKRGVVTE"
     gene            complement(6912..>7106)
                     /locus_tag="PKK81_09905"
     CDS             complement(6912..>7106)
                     /locus_tag="PKK81_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33236.1"
                     /translation="QDAMGDFDRCRPAATLRDAVELAAREAQPGEVVLLSPACASFDM
                     FADYQDRGRQFKALVQGLPA"
BASE COUNT         1422 a         2482 c         1906 g         1296 t
ORIGIN      
        1 gcgatgcccg tatagaccga attgatctgc acggccgcca tgagctccgc ttcctcaacc
       61 gccttcttga tcgactccac cgtactctcg atgttgacca ccacaccctt gcgaagcccg
      121 cgcgaggggc aggagccgac gccgatgata ttcagcgggc cctcctccgg cacttcggcg
      181 acaatcgcac agatcttggt ggtcccgatg tcgagtccga ccaggatatg atcccgctta
      241 gacactcggc tcaccccctt ccccgaacaa tgacgcggtc ggcaaagcga agatcgattt
      301 cattcgccct ggcgccctcg ccgtcaaagg ccacatcacg cagtgcgggc cgcattttga
      361 gaaaccggta ccattgctga ttcatcgacg actcactgaa ttgaaacgtc acaccctgga
      421 cctcgactac cagattattg agattcccca cattgatatc cggccgtcct ccggtcgtct
      481 gtccgaccat gcgggccagg gccgcgccga cctgcacggg gcgacgatcg tccggttttc
      541 cctgcaccag ccgcttgccg tcgacaccgg ataacatcgg tagggtcgga tcatccgccg
      601 acccgagatg cgccagcaag aacccgtccg aatcggccag gaggttctcc gccaaggtac
      661 gcaccaccac cgcaggctcc cgctcgacga tgtcgatatg aatttcgtgc aagggcttca
      721 gcaccaccgt cgcatccttg atccagggat gggtcttgat ccggtcggcc aaccaggacg
      781 ggttaatgga gtagagcgcg gtatcaggct tcaacgccaa tctcccgatc acttctttcc
      841 tcgtcacatg gtgcaacccg ctcaccgaca cggagcgcac cagaaaccat tcgcgcgtca
      901 gaggccccac ttcccgtgcc aggacgaaca gcccagaaca cccgccgacc agaacggtca
      961 ccgtggcaac cacccgcaag gtaatcagca ggccccgccc cagcctggac cagtacccgc
     1021 ccgtctcacc ggcccgatgg cggcccagcg cggattccga acgggcattc gcccgcgggt
     1081 tcactttgac cgggcgtacc ttgcgccacc gcagcgacat cgctacctca cggctcgaaa
     1141 tgatccgact ccggcccgcc cgagcgcgga ctggagaatc cgctccgtca aggcgtcgta
     1201 cgacaatccg gccttccccg ccgccatcgg cagcagactc gtctccgtca ttcccggcac
     1261 ggtgttgatc tcaagcacaa acggcttgcc cttgggcgtg atacggaaat cgacccgggc
     1321 tgccccgctg catcccaagg cctgataggt ccggatcgcc agttgcttga tctgccgagt
     1381 gactgcggcc gggagcggcg ccgggcacag gtactgggtt cgacctttct catacttggc
     1441 cgagaagtca taaaatccat ccggcgccac gatctccacg gccggcaacc cggtcaccac
     1501 cccgtcgggg ccgcccagaa gcgacaccgt gacctcatgt ccctggataa acgcctcgac
     1561 catcgcttct tcgtcatacc ggtgcgccgt acgaagcgcc tctttccatt gcgacggctt
     1621 ccggacaatg gtcactccga tcgtggatcc ctggctcgcc ggcttgacga ccaccggcaa
     1681 cttcaactta caggtgctca gcactcgatt cagactagga gccgtgccgc gaagcaccac
     1741 cgtcccgcgc gggaccggaa tgccctgagc ggccaattcc gtcttggtca ccaccttatg
     1801 catcccaatg gcactggccc ggacaccgga acccgtatag ggaatgccga gggtctccaa
     1861 aaatccctga atcgcgccgt cttcgcctcc cggtccgtgc aacgccagaa acccgacctc
     1921 aaccgcctgc tcctgcagcg tcttggacag atccggaccg acatcgatgg ccaccgcgtc
     1981 atagccgctt cgcaccagcg agcgatacac agcctccccg gtcttcagcg acacttcccg
     2041 ttcagacgat tgaccgccca tcaagacccc gatttttgaa cgtgtcagcg gcttccgttc
     2101 cgtcacatcc actcccttca tcgatccgcg tcaggcctca ccgaccacct tcagttccaa
     2161 ttccagcttc acgccggaca cttttttcac ggccgcccga accttcctga tcaacgccag
     2221 cacatcggcc gcgcgcgcat gacccacgtt caccatgaag ttcgcgtgtt tttccgagac
     2281 ctgcgcatct ccgatgcggg cccccttcag gcccgccgcg tccaccaggc ggcctgcgga
     2341 gtcctgcgga gggttcttaa acacacaccc ggcactcggc aacgtgagcg gctgagtatt
     2401 cttgcgatac tgaagatact ccttcactgt cttttcaatc cggtcgtgat cgccgggcct
     2461 caactgcacc cagactcccg ccacgatgcc gcgcggcaga tgcgcccgtc gataactgaa
     2521 cggaatatcc gcggcgggaa tatccaccac ccggccgcga ggatccacca tccggacggc
     2581 cttgacggac tccttcattt cgcccaagcg cgtgcctgca ttcatcacga cgcaccccgc
     2641 cacggtcccg ggaatcccag cgccccattc caagccggcg agcgaccgtc gaatggcata
     2701 tccgatcaac gtcggcatgc ccacgccccc ttctgcatag agcacgtggt ccggttcctg
     2761 gcggatggct ttgaattgcc gcaggctaac cacgatcccg cggatgcccc cgtcacgaac
     2821 gagcagattg gtccccccca ccacaaagac cggaatgtgt tccgcacgag cctgtgccac
     2881 cacccggcaa aggtcctcca catcggccgg ctccaccagc acttccgccg ggccaccgat
     2941 gtggaatgaa gtataggcct gcagcgaggc ctcgtacgtg attgttccac gaaccccttc
     3001 aagaatacgc gcccagtctt tcctggtcct tgccgcatga tctttgcccg ccacacgtga
     3061 cactgttgtt cgcctcatcg ccccctaagc cggcaatcgc tcaagaatcg ccaatcccgt
     3121 tttccagata tcccctgcgc caagggtaat cacgagatcc ccggatttca gggtcggcag
     3181 cacctgctcc gccagggtgt ccttgcgctc cacgaaagtg gccgaaggat gcccggccgc
     3241 ctgcaccgcc tccaccagtt tcgcgccgga cacgccaggg atcggctgtt cacccgccgc
     3301 ataaatttcc gtcatgaaga gtgcatctgc ctggtcgaac gcatgggcga attcctgcat
     3361 caaatcgcga gaacggctgt agcgatgcgg ctggaacagc acgacgacgc gtcgatccca
     3421 cccttgcttc gcggcggcga tcgtggcccg cacctcggtc ggatgatgcc catagtcgtc
     3481 caccaccatg atgccggcct tctctccgcg cagatggaag cgccgctcca ccccgctgaa
     3541 cgccgcgagg cctttccgga tcagatcgac cggaatatcc aactccatgc cgatggcaat
     3601 cgccaccaag gaattcgaca cattgtgaat gccgggaatg gacaaccgga acggaccgag
     3661 gttgcgtccg cggaagaaca cccgaaactc agacccccat tgccgcaggc tgatatcggt
     3721 ggcgcgaaag tcgggaacat ggccggggtg ttcgtgcaga ccgtaggtct gatagcgctt
     3781 cactacgcgc ggcagtagat ttcgtaaccg ttcatcgtcg gagcacaaca cggccaggcc
     3841 gtagaacggc accttgttca cgaattccag aaaactgtcg ttcagcctct ccatggtccc
     3901 gtagtggtcc aggtgctcac ggtcgatatt ggtgacggcg gcaatggtcg gtgcgaggcg
     3961 cagaaacgac ccgtcacttt catccgcttc cgcaatgagt agatcaccgc gccccagccg
     4021 tgcatggctc ccaagggcat tcacctttcc gccgatcacc atggtggggt ccaatccgcc
     4081 ctgtgcgagg acattggcca ccatcgaggt cgtcgtcgtc tttccatgag cgccggcgat
     4141 cgccacgccg aacttgagtc gcatcagctc cgcgagcatc tccgcacgcg ggatgaccgg
     4201 gatctgccgc gctttcgccg ccaccacttc agggttggtc gccgccaccg cggaagagat
     4261 tacgaccacc tgcgcgtcac cgatattgga ctcatggtgg ccgatcgcga tgcgcccccc
     4321 tagttcctca agccggcgcg tcgtttccga ctgactgagg tccgagccgc tgaccttata
     4381 cccgagcgtc aacaacacct cagcgatgcc gctcatcccg gatccaccga tgcccactaa
     4441 atgaatatgt tgtgtctttc tgaacatcgc cgtccgcctt agctcctgtc ctcgaaatga
     4501 tcgctgccca ctgctcctat cctgttgctg tgtctcccgc tctcgttgtt catagggcgc
     4561 gagcgctgtc gctggcctca tggcgcctcc ttatgacgtc gtaacactca cgaacgatgg
     4621 tttcggctgc atcgctgcgt cgcatattcc aactctgttc actcatggtc tgaagccgat
     4681 gcggctcctt caaaatctgc atgacggttt gcgccagtcc gggcccggtc aattccgctt
     4741 gcggcaggag cactgcgccg cccgccttgg ccatgacctc ggcattccgc agttgatggt
     4801 tgtagatcgc cgtcggcagc ggaatcagaa tcgccgcctt tccgcagacg gtgagctcgg
     4861 caatcgtcat ggctccggcc ctcgcgacca caagatcggc gcctcgcagc accgtgggca
     4921 tgtcgtagag aaagggcacg acctgcgcgg gcattccagc ctgttcatac gcggccacga
     4981 ctctggcgtg atccgcctca cccgtttgat gggtgatggt aagctggtcc ttcatggagc
     5041 gaagcaacgg caaggcctcg atcaccgccg agttgatggc cttcgcaccc tgactgccgc
     5101 cgaagatcaa caggtgtcga gcggtgcgtg cctccgcttg ctccgcatcc ggagcggcag
     5161 attccacaaa cgcctgccga actggtgtcc ccactacgct ggtcttgcat cgatcgaaaa
     5221 atgtcaccgt cgattcgaag gccaggaaca cccgctgcac gaacggcgca accgccttgt
     5281 tcgccatgcc gggataggcg ttcggctcga gaatcacacc ggggatccgc cgtagaaacg
     5341 ccgccaagag catcgccgga ctggtgtacc cgccgacgcc gaacacgaga tcggcactct
     5401 gcttcttgag gatccccagt gattgccata aactttttgg cagggtgatc agggccttca
     5461 ggatctccag cagactcttg cccatcaaag gattggcatc gatgcactgc aagggaaaac
     5521 cttcgtgggc cagcactttg cgttcgattc cacgcgtcgt gccgacaaac agaatccggg
     5581 tggatggatc gcgcttcaaa aactcgcgcg cgacggcaat ggccggatac agatggcccc
     5641 ccgttccgcc tgccgcaatc accacattca ttccgtcacc accccgcgct tgcgcaccac
     5701 ccgcggccca ctgctgtcct gcccgccctg ccggtcgcgt gaaatgctca acagaatccc
     5761 cacgccgaac aaattggcca tcaatgagga gccgccgtag ctgacaaagg gcagggtcaa
     5821 gcctttggtc gggagcagtc cggtgaccac tccggcattg accaaggcct gcatgccgac
     5881 gaggagcgtg atgcccattg ccaggtgccg accgaacggg ttgcgagccc gcctcgcgat
     5941 ctgaaagccc ttgatgacga acagaccgaa cagcagcacg atggtcacgg tgcccatcag
     6001 ccccaattct tcgccgacca gggccagcac aaagtcggta tgcgcctccg gcaagaaaaa
     6061 caacttctgt ttcccttcgc ccagccccac accgaacggg ccgccgctgc cgaacgccag
     6121 aaaggattga tggatttgat atccggaacc ggtcggatcc ttggccccgt acagatattc
     6181 gatcactcgt cgacgccgat aaggagaccc cagaatcaag gccgccaccg tcggcagcgc
     6241 acagagcgcc aggaggccca ggtgtttgat tcgcgcccct gccaggaaca gcatcgccac
     6301 gaccaccagg cccatcacca cgaccgttcc cagatcgggc tccagcaaaa ccaagccgct
     6361 cagcaacccc aacacgatca gcggcggtaa gaggcctcga gaaaaattgg tgatctgatc
     6421 ctgcttcttg gtcaggtagg ccgcgatgta tatggccacc gcgtacttcg ccagctcggc
     6481 cggctgaatg ttgagcggac cgaggtgcag ccatcgccgc gccccctttg ccacgctgcc
     6541 gaacgacgga acgagcacca acactagcag caccgtcgtc ccgaacagca gcggaatcgc
     6601 caactttttc catatggtgt agtcgatctt cgaaatgaga tgcatcacca agaggccggc
     6661 gcccaaccag gccaactggc gcttcaggaa gtaccagggg tcgtgaaacc gattccccgc
     6721 gaccaccgcg ctggcactga acaccatgac cactcccacc agggcaagaa tgagcgtcac
     6781 ggccagcagg gccggatcga ccggcacgcg tttggtcgcc cgctgggtcg aggtcgacca
     6841 gggcaacatc agtgttccga gcgcatgctg tgccatcgat cagctgtcgt tcctcccaca
     6901 cggctcccgc tttacgccgg cagcccttgt accagggcct taaactgccg gccccggtct
     6961 tgataatccg cgaacatgtc gaaactggcg caggccggcg agagtaagac cacctcaccg
     7021 ggctgcgcct cgcgagcagc aagctccacg gcgtcgcgga gggtcgctgc aggacgacac
     7081 cggtcaaaat ctcccatcgc atcctg
//
LOCUS       DAOQRM010000125         7056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10443_length_7056_cov_20.824596, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000125 DAOQRM010000000
VERSION     DAOQRM010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10443_length_7056_cov_20.824596"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /gene="hyfB"
                     /locus_tag="PKK81_09910"
     CDS             <1..1691
                     /gene="hyfB"
                     /locus_tag="PKK81_09910"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HNV33237.1"
                     /translation="GSLLNGFLLSMTLVVLADNGFFFLILWEVMSLLSYVLVVTEHEK
                     PGVREAGLFYLIMTHVGTAFIILTLLIFSQETGSFSFEAFRHPDQQLPEGLRSIAFFT
                     ALIGFGAKAGIVPLHVWLPYAHPAAPSHISALMSGVMIKTAIYGLIRVYFDFMGGVFP
                     WWWGFTLLVAGTVSALLGVMYALMEHDLKGLLAFHSVENIGIILLGIGAGMIFHTYGL
                     HELAALGLLAGLYHTINHAAFKALLFLGAGALQFSTHTRHIEEYGGLLRRMPWTGAFF
                     LIGAVSIAALPPTNGFVSEWLVFQSLFLSFQLPTLFLKLMLPIAAAMLALTGALALAC
                     FAKAFGMSFLAQPRSAHARHATEVPWSMRVGMGFLAGLCIVLGLAPMLVVPLLDRVTA
                     PLTGAAISSQVLALDGWVVAPVTVEFSSISTPVLAMLLVGAGALGLLIARLCGKGLRA
                     RYYKTWGCGLNLTPRMEYTATGFAQPIKQVFETIYQPTVKLEQEFLEQSKYFIKHQRF
                     ESHIEPVFEKYLYRPVVTLLLATADRLRIIQAGSLHLYLTYIFVTLVLLLLWAG"
     gene            1756..2712
                     /locus_tag="PKK81_09915"
     CDS             1756..2712
                     /locus_tag="PKK81_09915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNV33238.1"
                     /translation="MQSIIHIVLVVVQLVLLLTLSPFVVGLIRKVKARLQCRRGASLF
                     QPYADLAKLFRKQPVISSTTSWIFTAAPYIVFASTVAAGLLMPVFVSHTPLNFAGNII
                     ALVYLLALGTFFLILAGLDAGSAFGGMGSSREAIVATLAEPAMMLSILSIALTAGSTN
                     LSTIVHQSALMEGVVVAPPAHLMALAALFIVTLAETGRVPVDNPATHLELTMIHEAML
                     LEYSGRYLALMEWAAGIKLLVFLTLIVNVFAPWGIATSLAPSAIAVGLLVYLGKVAAL
                     ALVIGVIESMFAKLRLFRVPEMLGAAFILSLLALVFFYTLKG"
     gene            2717..3382
                     /locus_tag="PKK81_09920"
     CDS             2717..3382
                     /locus_tag="PKK81_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNV33239.1"
                     /translation="MQASTQLGSQLVDLCSVLLLLCCFAIVAQRRLSACVDLFALQSA
                     FLAATAALVAFLTGHKHIYFAAALTIVIKVIVLPRILRKVIERLNVSRELGMNINIPA
                     GLLICGALVIIAFFITQPIIPLGYLLTRDSLAIALAIILIGFFTMIARQKAITQMVGF
                     LVMENGVFLGATAAAYGMPLIVELGVFFDVLVAGLIVGIYTHRLQDTFDSVDTGTLTV
                     LKE"
     gene            3420..4952
                     /locus_tag="PKK81_09925"
     CDS             3420..4952
                     /locus_tag="PKK81_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HNV33240.1"
                     /translation="MWSVIVLLTGPVLAGLLSLVIHRARVLHVVNFSTMLALAVAETA
                     LTRQVLAEGSVTTLGGFVYVDALSDFILVIITAIGLSCSLYMWSYMDDRVARGIIAPK
                     RLGHFFFLFHMFLFAMVAATVANSLGVQWVALEGTTLATTFLIAFFRRRESLEAGWKY
                     LILCSVGIALALFGVVLTYYSSVRVLGDASSALNMTALIGVANQLDPNVLKLAFIFML
                     VGYGTKVGLVPMHTWLPDAYSEAPAPIAAMLAGVLETVAVYTVLRSKALVDQALPPEF
                     TGNLLLTFGLLSFIVASLFILIQHNYKRLFAYSSIEHMGLAMIGFGVGGMIGTFGGLF
                     HLLNHAVAKALAFFVAGNIHRRFDTLEIDGVRGLARAQPITAVAILVAGCALVGLPPF
                     SPFISELLVVSAVAAQDFSSDTMHVGRFVTMTISDEMRSLGIVALFLFFAVVLFGGFM
                     FRVGAMVWGTPPAGVAQGESWTAGHVPLMIMIVALLGLGFVLPDPIQTLLTRAVNVIV
                     VR"
     gene            4957..6549
                     /locus_tag="PKK81_09930"
     CDS             4957..6549
                     /locus_tag="PKK81_09930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNV33241.1"
                     /translation="MIDARPAVEQMQAAFPQALIEVCAVHGIPMLRLKKQDLPTVAHF
                     LHTNPNLRGSLSLLWAVDHRPREARYELCYLFTLAERKDWLLLATDLHGDEREFPSIT
                     PHLHAAKWYEREIRDLFGLIPVGHPDLRRLVRHEHWPKGSHPLKKDFPWDRVLGRVQG
                     EYAFRKIHGEGVFEVPVGPIHAGIIEPGHFRFSVAGEPIMQLEVRHFWKHRGVEKLFE
                     QQRLTEAVPLSERVSGDTSVGHSLAYCQAVETLQGVEVSPRARYLRTLFLELERLHNH
                     IGDVGAICNDTAYALAHAHCGRMKEQLMQLNDRLTGSRFLRGVNCVGGVALDLSGLQL
                     AQVEVELNTLEQDFSAIEKIVFANASLTERLENTGILTEGIAWDHAVIGVVGRASGID
                     RDLRRDRPFAAYDVLQPKVALYRYGDVRARMRVRLDEIHESIRLIREVRRGLPLGRLV
                     TRPMHEAQPGVWALSAVEGWRGEILYVVMAGDAGRIHRCKVRDPSFVNWPAIQWAALG
                     NIVPDFPLINKSFNLSYAGNDL"
     gene            6602..>7056
                     /gene="nuoB"
                     /locus_tag="PKK81_09935"
     CDS             6602..>7056
                     /gene="nuoB"
                     /locus_tag="PKK81_09935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNV33242.1"
                     /translation="MFRILKKSLQTGVVTGHHPAGAAPSEPVSQDAIEKAKPFRRSLT
                     IREVDTGSCNACEMEMNALANPVYDVERFGVHIAASPRHADALVVTGPVTVNMERALK
                     DVYKATADPKIVIALGDCAVNCGLFKGSYAVTGPVDRHIPVDVRISGCPP"
BASE COUNT         1107 a         1997 c         2233 g         1719 t
ORIGIN      
        1 tgggctccct cctcaacggg tttttgctct ccatgaccct ggtggtcctg gccgacaacg
       61 ggttcttctt cctgatcctc tgggaagtca tgtccctgct gtcctatgtg ctggttgtga
      121 cggagcacga gaagcccggc gtccgcgagg ccgggttgtt ctatctcatc atgacgcacg
      181 tcggcaccgc gttcatcatc ctcacgttgt tgatcttttc ccaggaaacc ggttcattct
      241 ccttcgaggc gtttcgccac cccgaccagc aacttcctga gggattacga tcgatcgcgt
      301 ttttcacggc cttgatcgga ttcggcgcga aggccggcat cgtcccgttg catgtgtggc
      361 tgccctacgc gcatcccgcc gccccgtccc acatctcggc cctgatgtcg ggggtcatga
      421 tcaagaccgc catctacggg ttgatccggg tgtacttcga tttcatgggc ggggtattcc
      481 cctggtggtg gggcttcacg ttgttggtcg ccggtacggt gtcggcgctc ctgggtgtca
      541 tgtatgcctt gatggaacat gacctgaagg gcctgctggc gtttcatagt gtggaaaaca
      601 tcggcatcat tctgctggga atcggcgcag ggatgatctt ccacacctat ggcttgcatg
      661 aactggctgc cctcggattg ctggcgggcc tgtaccacac tattaaccac gcggcgttca
      721 aggcgctgtt gtttctgggc gccggagcgt tgcagttctc cacccatacg agacacatcg
      781 aggagtacgg cggcttgctc cggcgcatgc cctggacggg cgcctttttc ctgatcggcg
      841 ccgtctccat tgccgcgctt ccgcccacca acggtttcgt cagcgagtgg ctggtctttc
      901 agagtttgtt tctcagcttc caactgccga ccctgtttct caagttgatg ttgccgattg
      961 ccgccgcgat gttggctttg acgggcgcgc tggcgctggc ttgttttgcg aaggctttcg
     1021 ggatgtcgtt tctggcgcag ccgcgcagcg cccatgcccg ccatgcgacg gaagtcccct
     1081 ggtcgatgcg ggttgggatg ggctttttag cagggctctg cattgtgctg gggctggcgc
     1141 ccatgctggt cgttccgttg ctggatcggg tcacggctcc attgaccggt gcggccatca
     1201 gttcacaggt gctggcgctc gacggctggg tggtggcgcc ggtgacggtg gagttttcga
     1261 gcatctcgac gccggtgctc gccatgctct tggtcggggc gggggcgttg gggttgctga
     1321 ttgcccgact ctgcgggaag gggctgcgcg cgcgctatta caagacgtgg ggctgcgggc
     1381 tgaatctgac gcctcgcatg gaatacaccg cgaccggctt cgcgcaaccg atcaagcagg
     1441 tctttgaaac catctaccag ccgacggtga agctggaaca ggagtttctg gagcaatcga
     1501 agtacttcat caaacatcag cgtttcgagt cgcacattga accggtcttc gagaaatacc
     1561 tctatcggcc ggtggtgaca ttgctgctgg cgacagccga ccggctgcgc atcattcagg
     1621 ccggcagtct ccatctgtat ctcacctaca ttttcgtgac gctggtgctg ttgttgttat
     1681 gggcggggta gggagagccg gcgggatgtg tgtatgtgtc gtgcctggtc gttcgatcat
     1741 tgagtgacgg gagctatgca gtcgatcatc cacatcgtac tggtcgtcgt tcagctcgtg
     1801 ctgttgttga ccctctctcc gtttgtcgtc ggcctgatcc gaaaggtcaa agcacgtcta
     1861 cagtgccgcc gcggggcgag tctgtttcag ccctacgccg atctggccaa gctgttccgg
     1921 aagcagcccg tcatctcttc gacgacctcc tggattttta ccgcggcgcc gtacattgtc
     1981 tttgcctcga ccgtagcggc ggggttgttg atgccggtat tcgtctctca cacaccgctc
     2041 aattttgccg ggaacatcat tgcgctcgtc tatctgctgg cgttggggac gttctttctg
     2101 atcctggcgg ggctcgatgc aggatcggcg ttcggaggga tgggcagcag ccgcgaggcg
     2161 attgtcgcga cgctggccga gccggccatg atgttgtcta tcctttccat tgcgctgacc
     2221 gccggctcca cgaatttgag caccatcgtg catcagtccg cgttgatgga aggggtcgtc
     2281 gtggccccac ctgcgcattt gatggcgctg gccgctctgt tcatcgtgac gctggcggaa
     2341 accggccgtg tcccggtgga caatcctgcg acgcacctgg aattgacgat gatccacgag
     2401 gccatgctgc tcgagtactc cgggcggtac ctggcgttga tggaatgggc ggccgggatc
     2461 aagttgctgg tgttcctgac cttgatcgtg aatgtctttg cgccctgggg tatcgccacg
     2521 agtctggcgc cgtcagccat cgccgtcggt ctcctggtgt atctcggaaa ggtggctgcg
     2581 ctggctcttg tgatcggtgt gatcgaatcg atgttcgcga agctgcgatt gttccgggtg
     2641 cccgaaatgc tgggcgcggc gttcattctg tcgctgctcg cgctggtctt cttctatacg
     2701 ttgaaagggt aatgcggtgc aggcttccac ccaattgggc tctcaactcg tcgatctctg
     2761 ctcggtcctg ctgctgctct gttgttttgc catcgtggcg cagcgtcgat tgtcggcctg
     2821 tgtggatctg tttgcgctgc agtccgcgtt tctggcggcg actgccgcgc tcgttgcgtt
     2881 tctgaccgga cataagcaca tttatttcgc ggcggccctg acgatcgtca tcaaagtgat
     2941 cgtgctgcct cgcattctcc ggaaggtcat cgagcgtctg aatgtctccc gtgagctggg
     3001 gatgaacatc aacattccgg cgggcctgct gatctgcggc gcgttggtga tcatcgcctt
     3061 ctttatcacg cagcccatca ttccgcttgg ctacctgctg acgcgcgatt ctctggccat
     3121 tgcgctggcc attattctga tcggattttt caccatgatt gcccggcaga aggcgatcac
     3181 gcagatggtc ggctttctag tcatggagaa cggagtgttc ctgggagcca cggcggccgc
     3241 gtacggcatg ccgcttatcg ttgaattggg cgtcttcttc gatgtgttgg tggccgggtt
     3301 gattgtcggc atttacacgc atcggctgca ggatacgttc gatagcgtag acaccggcac
     3361 gctgacggtg ctgaaggaat gagtcggttg atgacgcatg gccggcgagt aggtgctgta
     3421 tgtggtcggt gatcgtcctg ctgacggggc cggtactcgc cggtctgctc agcctggtaa
     3481 tccaccgcgc ccgggtcctg cacgtcgtga acttttcgac gatgctggcg ttggccgttg
     3541 ccgagacggc gctgacgagg caggtgctgg ccgagggatc ggtgacgaca ttgggcggct
     3601 ttgtctacgt ggatgcgctc tcggacttta ttctggtgat catcacggcc atcggcttgt
     3661 cctgttcgct ctacatgtgg tcctacatgg acgatcgggt cgcccggggc atcatcgcgc
     3721 cgaagcgtct cggtcacttc ttttttctct tccatatgtt cctgtttgcg atggtcgccg
     3781 cgacggtcgc gaacagcctc ggcgtgcagt gggtggcgtt ggaggggacc accctcgcga
     3841 cgaccttctt gatcgcattt ttccgcaggc gggaatccct cgaagccggt tggaaatatc
     3901 tgatcctctg ttcggtgggc atcgccctcg cgctcttcgg cgtggtgctc acctattact
     3961 cctctgtgcg ggtccttggc gatgcgagct cggcgctcaa tatgaccgcg ctgatcggcg
     4021 tggccaacca attggacccc aatgtgctca agctggcgtt tattttcatg ctcgtcgggt
     4081 atggcacgaa agtgggcctg gttcccatgc atacctggtt gcccgatgcc tacagcgaag
     4141 cgccggcgcc gattgcggcg atgttggccg gggtgttgga gacggtggcg gtctataccg
     4201 tgttgcgcag caaggcgctg gtggaccagg cgcttccccc ggagttcacc ggcaatctgc
     4261 tgctcacctt cgggctgctc tcgttcatcg tggcctctct gttcatcctg attcagcaca
     4321 actacaagcg gctgtttgcc tattcgagta tcgaacatat ggggctggcg atgatcggct
     4381 tcggagtcgg cgggatgatc ggcaccttcg gcggcctctt tcacttgctc aatcacgccg
     4441 tggccaaagc gctggccttt tttgtcgcgg gcaatatcca tcggcgattc gacaccttgg
     4501 aaatcgacgg ggtgcgaggg ctggcgcgtg cgcagccgat taccgccgtg gcgattctcg
     4561 tggcgggttg cgcgttggtc ggcttacctc ccttctcgcc gttcatcagt gagttgctgg
     4621 ttgtgtcggc agtggccgcg caagactttt cctccgatac gatgcatgtc ggacggtttg
     4681 tgacgatgac gatctcggat gagatgcgca gcctcggcat cgtcgcgctg tttctgtttt
     4741 ttgccgtggt gttgttcggc gggtttatgt ttcgcgtcgg ggccatggtg tggggcactc
     4801 ccccggccgg tgtcgcccag ggagagtcgt ggaccgccgg ccatgtgccg ctgatgatca
     4861 tgatcgtcgc gttgttgggg cttggattcg ttcttcctga tccgattcag acgctgctga
     4921 ccagggccgt caacgtgatt gtggtgaggt gaccgtatga tcgacgcacg tccggcggtc
     4981 gagcagatgc aagcggcatt tccgcaggcc ctgatcgagg tgtgcgccgt gcacggcatc
     5041 ccgatgctca ggctgaagaa gcaagatctg ccgacggtcg cccattttct gcacacgaat
     5101 ccgaatctgc gaggatcgct gtcgctgctc tgggcggtcg atcatcgccc tcgcgaagcg
     5161 cgctacgaac tctgctatct cttcacgttg gcggaacgca aagactggct gctgctcgcg
     5221 acggatctcc atggcgatga gcgtgaattt ccatcgatca ctcctcacct tcatgcggcg
     5281 aaatggtacg agcgggagat ccgggatctg ttcggactga ttccggtcgg ccatcccgat
     5341 ctgcgtcgac tcgttcgtca tgaacattgg ccgaaggggt cccacccgct gaaaaaagat
     5401 tttccctggg atcgggtgtt aggacgggtg caaggggagt atgctttccg gaaaattcac
     5461 ggtgaggggg tcttcgaggt tcccgtcggt cccatccatg cgggcatcat tgaaccgggc
     5521 catttccgat tttcggtggc cggcgagccc atcatgcagc tcgaagtgcg ccatttctgg
     5581 aaacatcgcg gcgttgagaa attgtttgag cagcagcggt tgaccgaagc ggtaccgttg
     5641 tccgaacgag tctccggcga tacgagcgtc ggccatagcc tggcctattg ccaggccgtc
     5701 gagaccttgc agggcgtgga ggtctcgccc cgtgcccgat atctgcgcac gctgtttctg
     5761 gagttggagc ggttgcacaa ccacatcggc gatgtggggg ccatctgcaa tgacaccgcc
     5821 tatgccctgg cccatgccca ctgcggccgg atgaaagagc agctcatgca gctcaacgat
     5881 cgcctgaccg ggtcgcgttt tctgcgtggc gtcaattgcg tcggcggcgt ggcgctcgat
     5941 ctgtccggcc tgcagctcgc gcaggtggag gtggaactga atacccttga gcaggatttt
     6001 tcggcgattg aaaagattgt gtttgcgaac gcgtcgctca cggaacggct ggagaacacc
     6061 ggcatcctta cagaagggat cgcctgggat catgcggtga ttggtgtggt aggccgggcc
     6121 tccggtatcg atcgggatct gcgtcgcgac cggccctttg ctgcgtacga cgtgctgcag
     6181 ccgaaggtcg ccctctaccg ctatggtgat gtgcgcgcca ggatgcgggt gcgcctggat
     6241 gagatccatg agtcgatccg tctcattcgc gaggtccgcc gcggtctccc actgggccgg
     6301 ctcgtgaccc ggccgatgca cgaagcacag ccgggtgtat gggcgctgtc ggcggtcgaa
     6361 ggatggcgag gcgagattct gtatgtcgtc atggccgggg acgcaggtcg gattcaccgc
     6421 tgcaaagtgc gcgatccgtc ctttgtgaat tggccggcaa tccaatgggc ggcgctcggc
     6481 aatatcgtgc cggactttcc gttgatcaat aagagtttca atttatctta cgctgggaac
     6541 gatctctagc aatggtcgat taaattactg actcgctgat tacattcccg agggaggagc
     6601 catgtttcga atcctcaaaa aaagtcttca gaccggtgtc gtcacggggc accatcctgc
     6661 cggagccgca ccttcggagc cggtcagcca ggacgccatc gaaaaggcca agccgttccg
     6721 tcgctcgctg acgatccgcg aagtcgatac gggttcctgc aacgcctgcg agatggaaat
     6781 gaatgcgctg gccaatccgg tctatgacgt ggagcggttc ggggttcata ttgcggcgtc
     6841 cccccgccat gccgatgccc tggtcgtgac ggggccggtg accgtcaata tggagcgtgc
     6901 gttgaaagat gtctacaagg cgacagccga tcctaagatc gtcatcgcgt taggagactg
     6961 tgcggtgaat tgcggcctgt tcaagggaag ttatgcggtg acggggccgg tggatcggca
     7021 tatccccgtc gatgtgcgta tctccggttg cccccc
//
LOCUS       DAOQRM010000126         7048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10467_length_7048_cov_18.271843, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000126 DAOQRM010000000
VERSION     DAOQRM010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10467_length_7048_cov_18.271843"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKK81_09940"
     CDS             complement(<1..260)
                     /locus_tag="PKK81_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV33243.1"
                     /translation="MRPEQGTMQSRAQAMAALLELLTDQPVIICNGFPSREAHKLADR
                     PTHFYMIGSMGNAPAIALGVALSKPGKQVIVFDGDGNVLMGMG"
     gene            complement(257..754)
                     /locus_tag="PKK81_09945"
     CDS             complement(257..754)
                     /locus_tag="PKK81_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNV33244.1"
                     /translation="MIESDVFVQALENLGVNFFTGVPDSLLGGIIEELLTRKLYTPAV
                     REDEAVAMAAGAYMAGKVPAVLMQNSGLGTSLNTLISLNMMYRQPCILLVSWRGFEGK
                     DAPEHLVMGETMPQLLDTMRIPHRTLSEPTMMDDLRWVGQTVMKQRIPVALLIKKGII
                     KGLHP"
     gene            897..>1435
                     /locus_tag="PKK81_09950"
     CDS             897..>1435
                     /locus_tag="PKK81_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33245.1"
                     /translation="MPYAFSKCSPLRMASRLGLLVVLIFILTVPALAITMANDPHGFD
                     HLTWGVPLTDIPELTVTRSNSHTIDYQFRDRPPVYADIPVESVHLSTVDDQFARVTIR
                     YSGAKAHAQVLQFLERSYGKIERLPGQMMRGLNQQYTWRGPDTEISLTYEANRDRGFI
                     FIESRNLAPRFQDRMSDTA"
     assembly_gap    1436..1535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1536..1793
                     /locus_tag="PKK81_09955"
     CDS             <1536..1793
                     /locus_tag="PKK81_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33246.1"
                     /translation="FARVMVRYSGKEAHDRILSYLQERFGPLDQTPGQVAGGAVKFFN
                     WQGDESEIILRYDLRTSQGVIFFESEAFRAKFNEGNSPSTF"
     gene            1921..2568
                     /locus_tag="PKK81_09960"
     CDS             1921..2568
                     /locus_tag="PKK81_09960"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV33247.1"
                     /translation="MKPTNALSRNPGQDRQASLISSAASLFAAKGFKGTTTKAIARAA
                     GVSEALVFKYFPTKRALYTAILAEKAPLSELLHAVEEVARKRDDQRVFTLIAGYRIRP
                     GADPTMLRLLLFSALEGHELADMFFGKQHRVFYDYLAGYIRTRIEEGAFREIDPLLAA
                     RAFIGMVVHHRLLHEIFDVPLHCPHKEIVSTYVELFLNGLRHSSTPRKRGLSRVR"
     gene            2558..3751
                     /locus_tag="PKK81_09965"
     CDS             2558..3751
                     /locus_tag="PKK81_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV33248.1"
                     /translation="MSANLIRRHPIVTLGIMLFVAVVALVALRLSSGAKSDPRKNRIL
                     TVGTMSPIKQDLDVRLTYTADLIPNQLVNIFSRVDGYIAKIYVDKGDLVKANQLLVEI
                     DHTDYVHAVNQAKANLLSAKAKVVQQEAAVRNAALTLDRMQALIKDQFVSQQDLDTAL
                     VNRDAALALQDSLRAQVQQMDVALAQAVTNLAYASIRAPFAGYIAERNLDPGAYVSGT
                     TASTSTMSRGILSVHDVETVRTLIEVVEKDVPLVKVGQRADVRAEAYPNEVFEGTVTR
                     IVQALNRATRTMTVEVDLPNKDHRLKGGMFARVEVLVGKHPQAIQIPLDAVSRLEESQ
                     YVYVVKDGKAHQVPVELGARAENRVEVVKGLTGDEQVIVSGKDLVSEGAAVQTQPMDT
                     VKRES"
     gene            3836..7015
                     /locus_tag="PKK81_09970"
     CDS             3836..7015
                     /locus_tag="PKK81_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV33249.1"
                     /translation="MWLTLLALRNRIGILMLSLAMVILGATSLERLPVDLFPNIQVPV
                     AFVGVIYKGAPPLDIEQSVVYPIEKAVSSASNVEHVESFAKQGIGAVQVWFNWGADIN
                     VGQMEVMQRITQILNSLPPGILQPFIVKFDVSNIPVAFVTASGGDLDERALYDLAYNT
                     IAPQIEQISNVAAATVEGGKIRQININLDPALLQARGLSILDVVSAVKASNLILPSGD
                     IKAGNLDYNVFTNTQFKTVEPINDVVVKIDQRGNPVRVRDVGVLSDSSDIQTNVVRTD
                     GKRSVYLRVNKQPIANTVEVVDALRKAIPKMIGIPPGVQLGISFDQSIYIRQSIKNLI
                     EQALHGSLLAAAVILLFLRNLTSTLIISVAIPLSILVTFIVLYFTNQTLNVFTMGGLA
                     LGIGRLVDDSIVELENIQRHLNVDRNRWDAIVNAAREVAMPIFASTVTTVVVFLPMFF
                     IVGIARLLLIPLTLTIAIALFTSFFVSRTVTPALCYRFLKSEQEAHRSLPSWFVSIMQ
                     WSQRRYDALDEGYERSLRWVLAHRRTLLVSVVTIFVGSLALLPFIGTEFLPVSDESQF
                     RIVLRGPVGQRVEKTVDQVAEVERVLREKIPPDELEAIASSTGVLAQGRSSLFNPNTG
                     PHTSVISIYLSSPDKRRRNQVEIMNAVRPAILKLFPGVAMFFDPGGLVKRVTSFGSQK
                     SIDVEIYGYDFEKARTVIHQVQDLMHKIPGMADIEVSREENYPEVNVVVDREKAALLG
                     ISETDVANAVLFSLNGNGQTDPIIFTDPQNGNEYYISAWLAEEHRKDLSAIENVLLTT
                     KNGEPVLLKNLATLKLNAGPVKIERKYFQRVVHITANPVGRDLGSIAQDLESAFAQMQ
                     LPPGFSIRLAGQIQQQRETFEGLTYASVLALILVYMVMAAQFKSLIDPFIIMFSVPMG
                     IPGVIVILYLTNTTISTSSLMGIIMMLGIVVSNGVLLVDYTNVLRRRGLALSTAVVTA
                     SRTRLRPILMTSLATVVGLMPMALGLGTGSETNAPLARAVVGGLTVSTILTLFLVPTV
                     YTMLEERFPRRADQLGEERVQSELVSQQA"
BASE COUNT         1401 a         2232 c         1948 g         1367 t
ORIGIN      
        1 cccatcccca tgaggacatt gccgtcgccg tcgaagacga tcacctgctt gcccggtttg
       61 gacagcgcca cgccgagcgc aatggccggc gcgttcccca tcgacccgat catgtaaaaa
      121 tgggtcgggc gatcggcaag cttgtgggct tcccgcgagg gaaatccgtt gcagatgatg
      181 accggctgat cggtcagcaa ttcgagcagc gccgccatgg cctgcgcccg gctctgcatg
      241 gtgccttgtt cgggcctcat ggatgcaacc ccttgataat gcccttcttg atcaggagcg
      301 cgaccgggat ccgctgcttc atcacggtct gccccaccca ccggaggtcg tccatcatcg
      361 tcggttccga cagcgtccgg tgcggaattc tcatcgtatc gagtaactgc ggcatggtct
      421 cgcccatcac cagatgttcc ggggcgtctt tgccctcgaa cccccgccag gacaccagaa
      481 ggatgcaggg ctggcgatac atcatgttca gcgaaatcag cgtattcaac gacgtcccga
      541 gcccggaatt ctgcatgagt acggcgggaa cttttccggc catgtaggcg cccgccgcca
      601 tggcgaccgc ttcatcctca cgcaccgcag gggtgtagag cttgcgcgtc aacaattctt
      661 cgataatacc gcccagcaac gaatccggca cacccgtgaa aaaattcacg cccagattct
      721 cgagcgcttg cacaaacaca tcgctctcga tcatgaaccc tgctcctctt gtctcaccgg
      781 cccgggcaca agaggcgcat tatagccaag gggtcctcca ttcacaagaa cagcgccgac
      841 gagttgccgt caccgcgaga accgtgatag cgtttggtga gaaacgattg aaggccatgc
      901 cctacgcgtt ttcgaaatgc tcccccctcc gcatggcgag ccgcctggga cttctcgttg
      961 tgctcatctt catcctgacc gtgcccgcat tggccatcac aatggcgaat gacccgcatg
     1021 gtttcgacca tctcacctgg ggtgtgcccc tcaccgacat tccggaactg acggtgaccc
     1081 gatccaactc gcacacgatc gattaccaat ttcgcgatcg tccgccggtc tatgcggata
     1141 ttccggttga gagcgttcac ctctcaaccg tcgatgacca gtttgcgcgg gtgaccattc
     1201 gctacagtgg cgcgaaagcc catgcgcagg tgttgcaatt tctcgaacgg tcctatggca
     1261 agatcgagcg gctgcccggg caaatgatgc gtggcctgaa ccagcaatac acctggcggg
     1321 gaccggatac cgagatcagt ctcacctatg aggccaaccg ggaccggggc tttatcttta
     1381 tcgaaagtcg caacctcgcg ccgagatttc aagaccggat gtcggatacg gcggannnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttcgc gcgagtcatg gtgcgataca
     1561 gcggcaaaga ggctcacgac cggatactga gttacctgca ggaacgattc ggcccgctcg
     1621 accagactcc cggccaagtt gcgggcggcg ccgtgaagtt cttcaactgg caaggagacg
     1681 agagcgaaat catcctccgc tatgacctgc gcaccagcca aggcgtcatc ttcttcgaga
     1741 gcgaagcgtt tcgcgcgaaa ttcaacgagg gcaattcgcc ctcgacgttt tgacgccccc
     1801 tctaccggtt gcccagccgt cacagccgca ggacggaatg ctcgacatcc ttgtcttgtt
     1861 tcgccggcac atgatagcct gacaggtgag taaccactca ctcacctgat tgccgatcgt
     1921 atgaagccga cgaacgccct atcccgcaat ccgggccaag accgccaggc cagcctgatc
     1981 tcctccgccg ccagtctgtt tgcggccaag ggcttcaagg gcacgactac caaggccatc
     2041 gcgcgcgccg ccggcgtcag cgaagccctg gtgttcaaat atttcccgac caagcgggcg
     2101 ctgtacactg cgatcctcgc cgaaaaggcc ccgctcagcg agctcctaca cgccgtcgag
     2161 gaagtggccc gcaagcgcga cgatcaacgg gtcttcacac tcatcgccgg gtaccgcatc
     2221 cgtcccggcg ccgaccccac catgctgcga ctcctgctct tcagcgcgtt ggagggccac
     2281 gaactggccg acatgttttt cggcaagcag caccgcgtct tctacgacta cctggcggga
     2341 tacattcgga ccaggattga agagggcgcg ttccgcgaga tcgatccgct gttggccgca
     2401 cgcgccttca tcgggatggt cgtgcaccac cgcctgctgc acgagatttt cgacgtgccg
     2461 ttgcattgcc cgcacaagga gatcgtctcc acctacgtgg agctgttcct gaacggattg
     2521 cggcattcgt ccacacccag gaagcggggg ctgtcgcgtg tccgctaatc tcatcagacg
     2581 gcatccgatc gtgaccctcg gcatcatgct ctttgtggcc gtggtggcgc tggtcgcgct
     2641 ccgtctgagc agcggagcca agagcgatcc ccggaaaaac cgcatcctca ccgtcggcac
     2701 catgagcccc atcaagcagg acctggacgt acgattaacc tacaccgccg acctcatccc
     2761 caaccagctg gtgaatatct tctcgcgcgt ggacggctac atcgccaaga tctatgtgga
     2821 taaaggcgac ctggtgaagg ccaatcaact gctcgtcgaa atcgatcaca ccgactatgt
     2881 gcacgcggtg aatcaggcga aggccaatct cctgtcggcc aaggcgaagg tcgtgcagca
     2941 ggaggcggcc gtgcgcaatg ccgccctcac gctcgaccgc atgcaggccc tcatcaagga
     3001 ccagtttgtc tcacagcagg atctggatac cgcgctggtg aaccgcgacg cggcgctggc
     3061 cttgcaggac tccttgcgcg cacaggtgca acagatggat gtcgcgttag cccaggccgt
     3121 caccaatctg gcctacgcct ccatccgcgc cccgtttgcc ggctatatcg cggagcgtaa
     3181 tctggatccc ggcgcgtatg tgagcggcac caccgccagc acgtccacga tgtcgcgcgg
     3241 tattttgagt gtgcatgacg tcgaaaccgt ccgcaccctg atcgaagtcg tggagaagga
     3301 tgtgccgctg gtgaaggtcg ggcagcgcgc ggatgtgcgc gccgaagcct acccgaacga
     3361 agtctttgaa ggcacggtga cccgcatcgt gcaggccctc aatcgcgcca cccgcactat
     3421 gaccgtcgag gtcgacctcc cgaataagga tcatcgtctg aaaggcggca tgttcgcccg
     3481 cgtcgaggtc ctggtaggaa aacatcccca ggcgattcag attcccctgg atgcggtgag
     3541 ccggctcgaa gagtcacaat acgtctatgt cgtcaaggac gggaaggccc atcaggtgcc
     3601 ggttgagctg ggagcccgcg cggagaatcg cgtcgaagtg gtgaagggcc tcacgggaga
     3661 cgaacaggtg attgtctccg gcaaagacct ggtgagcgaa ggcgcggcgg ttcaaacgca
     3721 gccgatggat accgtgaaac gtgagtcgta aaaggtaaca tgtgaaggga cctcctgcgt
     3781 tgacggtgtg ctcacttcgt ttcacatcta acgtttcacc gctcacgtct caatcatgtg
     3841 gctgacactg ctcgcattgc gcaatcgcat cggcatcctg atgctgtccc tggccatggt
     3901 gatcctgggg gccacctccc tcgaacggct cccggtcgat ctcttcccca acattcaagt
     3961 tccggtcgcg tttgtcggcg tcatctataa gggtgcgccc ccgctcgata tcgaacagag
     4021 cgtggtctat cccatcgaga aggccgtcag ttcggcctcg aacgtcgaac atgtcgagtc
     4081 cttcgccaaa caaggcatcg gcgcggtgca ggtctggttc aactggggcg ctgacatcaa
     4141 cgtcggccag atggaggtga tgcagcgcat cacccagatt ctgaacagcc tgccgcccgg
     4201 cattctgcaa ccgttcatcg tcaagttcga tgtctcgaac attcccgtcg ccttcgtgac
     4261 ggcctccggt ggcgacctgg atgagcgagc cctctacgac ctggcctaca acacgatcgc
     4321 cccgcagatc gaacagatct ccaacgtggc ggcggccacg gtcgaaggcg gcaagatccg
     4381 gcagatcaac atcaatctcg acccggccct gctccaggcg cgcggcctct ccattctgga
     4441 tgtggtcagc gccgtgaagg cctccaacct gattttgccg tccggcgata tcaaagccgg
     4501 gaacctcgac tacaacgtgt tcaccaacac gcagttcaag acggtggagc ccatcaacga
     4561 cgtcgtcgtg aaaatcgacc aacggggtaa cccggtgcgc gtgcgtgatg tcggcgtgct
     4621 cagcgactcg tcggacattc aaaccaacgt ggtccgcacg gacggcaaac gctccgtcta
     4681 cctgcgggtg aacaagcaac cgattgcgaa cacggtggaa gtcgtcgatg ccttgcggaa
     4741 ggcgatcccc aaaatgatcg gcattccgcc gggcgtgcaa ttgggcatct ccttcgacca
     4801 atcgatctat atccggcaat cgatcaagaa cctcatcgag caggccctcc atggctcgct
     4861 gctcgcggcg gcggtcattc tgctgtttct gaggaacctg accagcacgc tgatcatctc
     4921 cgtcgccatt ccgctgtcga ttctcgtgac ctttatcgtg ctgtatttta ccaaccagac
     4981 cctgaacgtc ttcaccatgg gaggcctggc cctaggcatc ggtcgtttag tggacgattc
     5041 catcgtggaa ttggagaaca ttcagcgcca tttgaacgtc gaccggaacc ggtgggacgc
     5101 catcgtcaac gccgcccgcg aagtcgccat gccgatcttc gcctccacgg tcaccaccgt
     5161 cgtcgtcttc ctgcccatgt ttttcatcgt cgggatcgcg cggctcctgc tcattccgtt
     5221 gaccctcacg atcgccatcg ccctgttcac ctcgttcttc gtctcccgca cggtgacccc
     5281 cgcgctctgt tatcgcttct tgaagtcgga acaggaggcg caccgttcct tgccctcctg
     5341 gttcgtgagc atcatgcagt ggagccagcg ccggtacgac gcgctcgatg aaggatacga
     5401 acgcagcctg cgctgggtgc tggcccaccg gcgcacgttg ctggtttcag tggtcacgat
     5461 cttcgtcgga tcgcttgcgc tgctgccctt catcgggacc gagtttcttc cggtctcgga
     5521 cgaaagccag ttccggatcg tgcttcgcgg gccggtcggt cagcgggtgg aaaaaaccgt
     5581 cgatcaggtc gccgaagtcg agcgtgtgct acgggaaaag attccgccgg atgaactgga
     5641 agcgatcgcc tccagcaccg gggtcctggc gcaggggcgt tcgtccctgt tcaatcccaa
     5701 caccggcccg cacacctcgg tcatttccat ctatttgtcg tcccccgaca agcgacggcg
     5761 gaaccaggtc gaaatcatga atgcggtgag acccgcgatc ctcaagctct tccccggcgt
     5821 cgcgatgttc ttcgatccgg gaggcctggt caaacgggtc accagtttcg gttcgcagaa
     5881 atccatcgat gtcgaaattt acgggtacga cttcgaaaag gcccgcaccg tcatccacca
     5941 ggtgcaggac ctcatgcata agattccggg catggccgac atcgaagtca gccgggagga
     6001 aaactatccc gaggtcaacg tggtcgtcga tcgcgaaaaa gccgcgctgc tggggatcag
     6061 cgaaacggac gtggccaacg ccgtcctgtt ttcactgaac ggaaacggcc agaccgatcc
     6121 gatcatcttc accgaccccc agaacggcaa tgaatactac atcagcgcct ggctcgcgga
     6181 ggaacatcgc aaggacctgt cggccatcga aaatgtgctg ttgacgacaa aaaacggaga
     6241 gccggtgctc ctcaagaacc tggctacgct gaaactgaac gccggtcccg tgaagatcga
     6301 gcgcaaatac ttccagcgcg tcgtccacat taccgccaac cctgtcggcc gcgacctcgg
     6361 ctcgattgcc caggacctgg agtcggcgtt cgcccagatg cagttgccgc cgggattcag
     6421 catccggctc gccggacaaa tccaacaaca acgcgagacg ttcgaaggcc tcacctatgc
     6481 cagcgtcctg gcattgatcc tggtctacat ggtgatggcc gcacaattca aatcgttgat
     6541 cgaccccttc atcatcatgt tttccgtgcc gatgggaatc cccggcgtga tcgtgatcct
     6601 ctatctgacc aacaccacca tctccacctc ctctctcatg ggcatcatca tgatgctggg
     6661 tatcgtcgtc tccaacggcg ttctgctggt ggactacacc aatgtgctgc gacgccgcgg
     6721 cctggccctc tccaccgccg tcgtcacggc ctcgcgcacc agactgcgcc ctattctgat
     6781 gacctcgctg gctacagtgg tagggctgat gcccatggcc ctcgggctcg gcaccggcag
     6841 cgagaccaat gccccgctcg cacgcgccgt cgtgggaggg ctcaccgtct cgacgatcct
     6901 gaccctgttt ctcgtgccga ccgtctatac catgctagaa gagcgctttc cgcgtcgggc
     6961 ggatcagctg ggagaagagc gggttcagag tgagttagtg agtcaacaag cctagaaaat
     7021 gtcttcgcac aagagaacgg tctaaggc
//
LOCUS       DAOQRM010000127         7008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10554_length_7008_cov_17.622753, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000127 DAOQRM010000000
VERSION     DAOQRM010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10554_length_7008_cov_17.622753"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PKK81_09975"
     CDS             complement(<1..1085)
                     /locus_tag="PKK81_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNV33250.1"
                     /translation="MTTTNPAAPAAPLAPPDLARIAKTVATKLPFRGDVTGLTPLAGD
                     ASNRRYFRIALKGTPSALILMQLADPEGFKKSEEAVSGASAQIAELPFTNVLAHLQRT
                     GVTVPQLHYYDREAGLLYLEDFGDLTLAEACRDADRARLVALYRQAIDQLVLLQVKGT
                     QPPAPGCIAFHRGFDVPLLMWEFDHFLEYGIVARLGQPMKPADDKAVRAEFQRIAELL
                     AGQPQVFVHRDYHSRNLMVDGARLGIIDFQDALMGPATYDLASLLKDAYIELDDAVVD
                     GLVDHFLDGLAAQGQGWADRAAFRRLFDFTSIQRNLKAAGRFVYIDRVKHNPKFLADI
                     PRVLGYVKRNLSKYPELATLRQHLAPYV"
     gene            1220..2191
                     /locus_tag="PKK81_09980"
     CDS             1220..2191
                     /locus_tag="PKK81_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNV33251.1"
                     /translation="MHYVHLGRSGVRVSRLCLGTMNFGPQTSETDSFAIMDQALERGI
                     NFFDSANVYGWKVGEGVTEQIVGRWLSQGSGRREKIVLATKVYGRMGDWPNQSRLSAL
                     HIKRACEESLRRLQTDHLDLYQMHHIDRECPWEEIWQAMEQLCREGKVLYVGSSNFAG
                     WHIAQAQEMAKSRHFLGLISEQSLYNLLERTVELEVIPACQAYGIGIIPWSPLARGLL
                     GGALSTHTTGRRADEDVRKDIETHRAKLEAYEQFCKTVNATPATVALAWLLHQPAVTA
                     PIIGPRTMEQLTTALPALELTLTPDSLKTLDTIFPGPGGAAPEAYAW"
     gene            complement(2317..2760)
                     /locus_tag="PKK81_09985"
     CDS             complement(2317..2760)
                     /locus_tag="PKK81_09985"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNV33252.1"
                     /translation="MESNQMVSREIRTAPLVSPFSVLRYWGPVCVYAGLIFFGSSVSN
                     PPESVSSLMEKISDKILHLCEYGVFGALAYRACRHGAGAWVARHAVVVAVAGCALYGL
                     SDEIHQLFVPLRQGDPLDLVADSVGAALGAWTWRLMERRAVQSSL"
     gene            2918..5122
                     /locus_tag="PKK81_09990"
     CDS             2918..5122
                     /locus_tag="PKK81_09990"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNV33253.1"
                     /translation="MKTIQLCFLWHMHQPYYTDPLTGSASMPWVRLHATKAYFDMAFL
                     LERFPEARSTFNFTPSLLLQLEEFSTGRVRDLFLEYAQRPAAELTPTEKAFLIRHFFS
                     ANWATMVRPFPRYQELLVKRGVDVQGQDLDRLARQFSTQEFLDLQVWHNLAWFGYGSL
                     QRFPRLAELRTKNRGFTEEDKQEVLALQQTAIRQIVPMYTALQERGQIELTTTPFFHP
                     ILPLVIDSEFTRRARPDLPLPARFHAPADAEAQVRRAIDYHTHTFGRAPAGLWPSEGS
                     VCPELLPILGKTGIRWLATDEGILYRSLQMADQTWNRHYHLYQPYAVGTAEQPLTMVF
                     RDRDISDAFGFVYHKTTPESAADDVLRRIRGLAYDIPLENGLLAVILDGENPWEHYHD
                     GGERFLSLLFRAFEQDGLHIGHGIRVRLNTVSQALESVPPPQRLDQLHSGSWINQDFK
                     IWIGHQEDNRGWDLLQHTRARLVDLTPSLAPDKARAAWDELYAAEGSDWFWWYGDDFD
                     TDYKQEFDRLFRTHLRNVWTYAGVTPPDILNQPLVEARTPQGLDLVQLPLALITPTLD
                     GMVSNFFEWRGAGTINPTPPLGAMWKSEGLFTSILFGFDREHLYLRLDLDERSQTRQE
                     HCTADLFIGSGIQQYKLSFALTAEGADTFLLSRADESGLYRDRGSYRTICRRKILELG
                     IPFKELGIEIDTELRLTLTVSEHGMEIARYPHHSPATFNRPGDDFEATMWRV"
     gene            5226..6227
                     /gene="galT"
                     /locus_tag="PKK81_09995"
     CDS             5226..6227
                     /gene="galT"
                     /locus_tag="PKK81_09995"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNV33254.1"
                     /translation="MPELRRDPIVGRWVIISTERGGRPQDVSMPSAPLPSASMCPFCP
                     GQERLTPKEILAYRPHASEPDSPNWTVRVIPNKFPALHVEGDMGREGLGLYDRMNGIG
                     AHEVIIETPNHKEHLADLPAKRVEDVLWAYRDRILDLKKDLRLRYILIFKNQGATAGA
                     TLEHSHSQLIALPVVPTSVLEEIDGCRQHFQQKERCIYCDILRQESSEGTRVVLENPE
                     FVCLTPYAPRFPFEMWILPKRHAGYFEECQRAQFEFLAPMLGEALRRMDTVLARPAYN
                     FILHSSPLHEKTGDYYHWHIEIIPKLTQVAGFEWGTGFYINPVSPEEAAKALRDAEI"
     gene            6533..6685
                     /locus_tag="PKK81_10000"
     CDS             6533..6685
                     /locus_tag="PKK81_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33255.1"
                     /translation="MKVQNLEWKDETFSELDLQNQKYKRLAIGSALLFLSVVGLLIYQ
                     IIAGIK"
BASE COUNT         1516 a         2222 c         1972 g         1298 t
ORIGIN      
        1 acgtagggcg ccaagtgctg acgtaatgtg gccagctcgg ggtatttgga taaattccgt
       61 ttcacgtagc ctagaacgcg agggatgtcg gcgagaaatt tcgggttgtg tttgacccgg
      121 tcgatataga caaaccggcc ggcggccttg aggttgcgtt gaatactggt gaagtcgaac
      181 aggcggcgaa acgccgcccg atccgcccag ccctgcccct gggcggcaag gccatcgagg
      241 aaatgatcga ccagcccatc caccaccgca tcatccaact cgatgtaggc atctttcagc
      301 aacgaggcca gatcgtaggt cgccgggccc atgagggcat cctgaaaatc gatgataccg
      361 aggcgggctc catcgaccat gagattgcgt gaatggtaat cgcgatggac gaacacctgc
      421 ggctgcccgg ccagcaattc ggcgatacgt tgaaactcgg cgcggaccgc cttgtcgtcg
      481 gccggtttca tgggctgccc gaggcgggcg acaatcccgt attcgaggaa gtggtcgaac
      541 tcccacatca acagcggcac atcgaatccc cggtgaaaag cgatacagcc gggggcgggc
      601 ggttgcgtcc ccttcacttg aagcagcacc aattgatcga tcgcctgacg atagagcgca
      661 accaaccggg cgcgatccgc atcacggcag gcctctgcca gcgtcagatc cccgaaatcc
      721 tccaggtaca aaaggccggc ttcccgatcg taatagtgca actgcgggac ggtgacaccg
      781 gtacgctgca gatgcgcgag cacgttggta aagggcagtt ccgcaatctg agcggacgcg
      841 ccgctgacgg cttcttcaga ctttttgaaa ccctccggat cggccaactg catgaggatc
      901 aatgccgatg gcgtcccctt caaggcgatc cggaagtatc ggcgattcga tgcgtcaccg
      961 gcgagcgggg tcaaccccgt cacatcgccg cgaaacggga gcttggtcgc aactgtcttg
     1021 gcgattcgtg caagatccgg cggggcaagc ggtgccgcgg gagcggcagg atttgtcgtt
     1081 gtcatacgat cggcattata gccaaacctt gctgaactgc cagcagaaag tcacctgctg
     1141 catgcccctc gtctgccgat tggttgccag ccctcaccac gagccggtat cctatctgct
     1201 ctaccccaag gagacgcaca tgcactacgt tcatctcggg cgctcaggcg tgcgggtcag
     1261 ccgcctctgt ctcggcacta tgaacttcgg gccgcagacc tctgaaaccg acagctttgc
     1321 gatcatggac caagcgctcg aacggggcat caactttttc gacagcgcca acgtctatgg
     1381 ctggaaagtc ggcgaagggg tgacggagca gattgtcggc cgttggcttt cccaaggcag
     1441 cgggcgacga gagaaaattg tgctggccac gaaggtctat ggccgcatgg gcgactggcc
     1501 caaccagtcg cgcctgtctg ccttgcatat caaacgcgcg tgcgaagaga gtctccggcg
     1561 tctgcagaca gaccatctcg acctctacca gatgcatcac atcgatcgtg aatgcccctg
     1621 ggaggaaatc tggcaggcga tggaacaact ctgccgcgaa gggaaggtgc tgtacgtcgg
     1681 cagcagcaat ttcgccggct ggcacatcgc gcaggcgcaa gagatggcga agtcacgcca
     1741 ctttctcggg ctcatctcgg aacaaagtct ctacaatctc ctggagcgaa ccgtcgaact
     1801 ggaggtcatc ccggcctgcc aggcctatgg gatcggcatc attccctgga gtcccctggc
     1861 gcgaggtctg ctgggagggg cgctgagcac gcacacaacc gggcggcgtg cagacgaaga
     1921 cgtccggaaa gacatcgaga cccatcgcgc aaagctagaa gcctatgaac agttctgcaa
     1981 gaccgtgaat gccacgccgg ccaccgtcgc cctcgcctgg cttcttcatc aaccggctgt
     2041 gacggccccc atcatcgggc ctcgcaccat ggagcaactg acaaccgcac ttccggcgct
     2101 ggagttgacg ctgactccgg acagcctcaa gacattggac acaatctttc ccggcccggg
     2161 cggcgccgcc ccagaggcct acgcctggta gaagttaaca acgaaaggca agctcagcgg
     2221 accatctgca tcgtcatcac gcagggaaag gagcgaggat cggagaaaca ccgcggcaag
     2281 aatcgatggg acaggaccgg gcgaaacgcg tcgaggctac agagatgact gaacagcgcg
     2341 ccgttccatg agacgccagg tccaggcccc gagcgcggca cccacggaat cggccaccag
     2401 atccagcggg tccccctgac gaagcggcac gaacaattga tggatctcat cactcaggcc
     2461 atagagcgca caaccggcca ccgccacgac gacggcatgc cgggccaccc aggcacccgc
     2521 tccgtgccgg caggcccgat aggctaaggc gccaaacact ccatattcgc agagatgcaa
     2581 gatcttgtcg gagatctttt ccattagcga cgaaacggac tccggcggat tggatacaga
     2641 agacccgaag aaaatgagtc cggcatacac acagaccggg ccccaatacc gcaggaccga
     2701 aaaaggggat accagcggcg ctgtacggat ctcgcgcgaa accatctgat tcgattccat
     2761 tctgccaggc tccccgtttc acaccaaagt cgatcccgta ccgcatgatc gctctcaccc
     2821 gggcggaaag aaaacaggcg ctcctcaatc ctgccctttc attgcaacac tcctacccct
     2881 atgacatact gccctggagt ctgcaagaaa cttcccgatg aaaaccattc aactctgctt
     2941 cctctggcat atgcatcagc cgtattacac ggacccgctc acggggtccg cgagtatgcc
     3001 ctgggtgcgt ctgcacgcca ccaaggccta cttcgacatg gccttcctgc tggagcgatt
     3061 tccggaggct cgctcgacct ttaacttcac gccgtcgctg ctgctgcaac tggaagagtt
     3121 ctccaccggc cgcgtccgcg atctcttcct ggaatatgcg cagcgcccgg cggctgagct
     3181 gaccccgact gaaaaagcct tcctgatccg ccatttcttt tccgccaatt gggccaccat
     3241 ggtacggccc tttcctcgct atcaggaact gctggtgaaa cgtggcgtcg acgtccaggg
     3301 gcaagacctc gatcgtctgg ccagacagtt ttcgacgcag gaatttctgg acctccaggt
     3361 ctggcacaat ctcgcctggt tcggatacgg cagcctgcag cgctttccac gtctggcgga
     3421 gttgcgcacg aagaatcggg gatttacgga agaggacaag caggaagtgc tggcgctgca
     3481 acagaccgca atccggcaga ttgtcccgat gtatacggcg ctccaggagc gtgggcagat
     3541 cgaattgacc accacgccgt ttttccaccc gattcttccg ctcgtgatcg actcggaatt
     3601 cactcgccgc gccagaccgg acctgccgct gcccgctcga ttccatgctc cggccgatgc
     3661 cgaagcccag gtgcggcggg caatcgacta ccacacgcac acgttcggcc gtgcaccggc
     3721 cgggctctgg ccctctgaag gatcggtctg tcctgagttg ttgcccatcc tgggcaaaac
     3781 cgggatccgc tggctggcga cggacgaagg tatcctctac cggtcgcttc agatggccga
     3841 ccagacctgg aatcgtcatt atcatctcta tcagccctac gcagtcggca ccgcggagca
     3901 gccgctcacc atggtgttcc gcgaccggga tatttccgat gccttcggct tcgtctatca
     3961 caagacgacc ccggagtcgg ccgccgacga tgtgctcagg cgaatccgcg gcctcgcgta
     4021 cgacattccg ctggagaacg ggctcctggc cgtgattctc gacggcgaga atccctggga
     4081 gcattaccac gacggcggtg aacgcttcct ctccctgttg tttcgcgcct tcgaacagga
     4141 cgggctccat atcggccacg gcatccgcgt gcgattgaac accgtgagcc aagccttgga
     4201 gtcggttccg ccccctcaac gcctcgatca gttacattcc ggctcctgga tcaaccagga
     4261 ctttaaaatc tggatcggcc accaggaaga caatcgcggc tgggacctcc tgcagcacac
     4321 gcgggcccgt ctcgtggacc tcacgccctc gttggcgccg gacaaggccc gcgcagcctg
     4381 ggacgaactc tatgccgctg aaggcagcga ctggttctgg tggtacggcg acgacttcga
     4441 taccgactac aaacaggaat tcgaccgcct gttccgcacg cacctgcgca acgtctggac
     4501 ctatgccggg gtgacgccgc ccgacatctt gaatcagcct ctggtcgagg cgcgcacgcc
     4561 gcaaggcctg gacctggtgc aactcccgct ggcgctcatc accccgaccc tcgacggcat
     4621 ggtctccaat tttttcgaat ggcgcggggc cggcaccatc aacccgacgc cgcctttggg
     4681 agcgatgtgg aagtccgaag gactcttcac ctccattctc ttcgggttcg accgcgaaca
     4741 tctctacctc cggctggatc tcgatgaacg atcccagaca cgccaggagc actgcaccgc
     4801 agacttgttc atcggctcgg gcatccagca atataagttg tcgtttgccc tcacggccga
     4861 aggcgcggac accttcctgc tctccagggc ggacgagtcc ggcctctatc gcgacagggg
     4921 ctcctaccgc acgatctgcc ggcgaaaaat tctggagctg ggcataccgt tcaaagaact
     4981 gggcatcgag atcgacacag aactacggtt gacgctgacg gtgtcggaac atgggatgga
     5041 aatcgcgcgg tatcctcatc acagtccggc caccttcaac cggccgggag acgactttga
     5101 agccaccatg tggcgcgtct gaacgacaat gctcaaggct ccctgttcaa tgaggtcgtc
     5161 accatccacc ccgtcattgg acatcgagca ttgcacagtg accattgaca actagaggag
     5221 tcgagatgcc tgaactgaga agagacccga tcgtcggccg ctgggtcatc atttcaaccg
     5281 aacggggcgg acgcccccag gacgtgtcta tgccgtcggc gccgctgccc tctgcgtcca
     5341 tgtgtccgtt ttgcccgggc caggaacgtc tgacgccgaa agagatcctc gcctatcggc
     5401 cccatgcctc ggagccggac agcccgaatt ggaccgtgcg ggtcatcccc aataagtttc
     5461 ccgcgctgca tgtcgaaggc gatatgggac gcgaaggcct cggcctctac gaccgcatga
     5521 acgggatcgg cgcccatgaa gtcatcatcg aaacccccaa tcacaaggaa cacctcgccg
     5581 acctgccggc caagcgggtg gaggatgtac tctgggccta tcgcgaccgg attctcgacc
     5641 tcaagaagga tctgaggctg cgatatattc tgatctttaa aaatcagggc gccacggcgg
     5701 gcgccacgtt ggagcatagc cactctcagc tcatcgcctt gccggtcgtg cccaccagcg
     5761 tcctcgaaga aatcgacgga tgccggcagc atttccagca gaaggaacgc tgcatctatt
     5821 gcgacatcct gcggcaggaa tcgtcggagg gcacccgcgt ggtgctggag aacccggaat
     5881 ttgtctgcct gaccccctac gcgccccgct ttccgtttga aatgtggatt ctccccaagc
     5941 gtcacgcagg atatttcgaa gagtgccaac gcgcccagtt cgaatttctg gcgcctatgc
     6001 tgggcgaagc gttacgccgg atggataccg tgctggctcg cccggcctac aacttcatcc
     6061 tgcacagctc gccgctgcac gaaaaaaccg gggactacta tcactggcac atcgagatca
     6121 tccccaaact cacccaggtg gccggatttg aatggggcac cggattttac atcaacccgg
     6181 tctccccgga agaagcggcg aaggccctgc gtgacgcgga aatctagcac ccactggatc
     6241 gcctccaggg aaatcgtgcg gtggcaccgc tcccgcacga tcacttcctc acattcctct
     6301 ggagtagcga tacttcaagg acaacgtatt ggacaaatcg ccggggacac gtgagttttg
     6361 tccagtatct ttccttgcta ttcacaagta cgacgaagta taaattccac gtaatctttt
     6421 cagggcattg ggggccgccg gacggcgaac gcccactcgc actacattca gacattcatc
     6481 cacgtgtaac gggatgggga ggcagcgacc accaggaagg taggagggta tcgtgaaagt
     6541 gcaaaacctc gaatggaaag acgagacgtt cagtgaactg gatctccaaa atcaaaaata
     6601 taagcgtctc gcaatcgggt ccgccctcct cttcctcagc gtggtcggct tgctgattta
     6661 ccaaatcatc gcgggcatca agtagccaac ggcagatcgt cgcaccctat gcgcgaaggt
     6721 tcgtactgct gcggctccat cggtgcgtgc ccttccttcc atccgaaatc gcctaccgca
     6781 ccagcgcaca tccaggcaac agcatcccga ccggattgca atcccgcaca actcagccgt
     6841 atcatcgcgc atgcttgttc atctcagtca cccgcaacgc caggttgaaa tcaaagggcc
     6901 caaacgcacc aaggagttgc tacgcgaact caatctggtg attgaggcgc atctcgtcat
     6961 ccggggcgat gaactcgtca cggaagatga aatgcttgcc gacgccga
//
LOCUS       DAOQRM010000128         6993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10588_length_6993_cov_19.813203, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000128 DAOQRM010000000
VERSION     DAOQRM010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10588_length_6993_cov_19.813203"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKK81_10005"
     CDS             complement(<1..484)
                     /locus_tag="PKK81_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33256.1"
                     /translation="MPTLYLIDGSAYIYRAFFALPPLANSKGLQTNAVYGFTTMLLKV
                     LRDHRPDYVAVVFDEKGPTHRHEAFKDYKAQRPPMPQGMSAQIPYIHRVVEALSLPVI
                     RQAGYEADDLIGTLARKGEVEGLDVVIVTSDKDMFQLLTPKTRIYDPVKDKWFGEADS
                     Q"
     gene            complement(543..974)
                     /locus_tag="PKK81_10010"
     CDS             complement(543..974)
                     /locus_tag="PKK81_10010"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HNV33257.1"
                     /translation="MISELLTQIESYVPANVLIWFAVSSVFMFVGTLIAIPIILMRLP
                     ADYFDVRTPRPWMENHHPILRVIGHVVKNVVGAIFLFAGFLMLFLPGQGVLTMLIGLS
                     LIEFPGKRRVEAKIVGQSTVLSTINAMRAKFDKPPLIIAPD"
     gene            1068..1463
                     /locus_tag="PKK81_10015"
     CDS             1068..1463
                     /locus_tag="PKK81_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33258.1"
                     /translation="MPPMRLNSARVTRACLQASGLAVLGFALSLYPVALSSHAAWAAD
                     GTTESELQIDITKIGLGKEVVITQGKKEWFMRVEVTPENSVVVRQEKEGERYLLDESE
                     MHDRAMTPAEVDVAIADFVNSVKTRQKVK"
     gene            1469..1888
                     /locus_tag="PKK81_10020"
     CDS             1469..1888
                     /locus_tag="PKK81_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33259.1"
                     /translation="MSGKPKFVIFAHNATYDKLHQVATLGLTAAAMGKDVIVVLLFWT
                     IKKLAEGKIDEIDFPPEYAASAEQVARLLKEKKVPKISEMFQEARLVGHFRLIACSAG
                     LEYMGVAAGAVERNVDEVLGLPSILSLTAQAETKLFI"
     gene            complement(1952..2611)
                     /gene="nth"
                     /locus_tag="PKK81_10025"
     CDS             complement(1952..2611)
                     /gene="nth"
                     /locus_tag="PKK81_10025"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNV33260.1"
                     /translation="MQAQEIHAAIRTVKREIARWPDPVVGVVARQSGRDPFLVLISCL
                     LSLRTKDKTTAEASERLFALASTPATMQTLTTPVIERAIYPVGFYRTKAKQIQQICAQ
                     LRERYQGRVPDKIDELLTLPGVGRKTANLVVTVGYEKPGICVDIHVHRISNRWGYVKT
                     KSPDETETALREKLPRKYWITFNDLLVPYGQHLCQPVSPLCSQCKIAAYCDRVGVTKS
                     R"
     gene            complement(2683..2877)
                     /gene="rpsU"
                     /locus_tag="PKK81_10030"
     CDS             complement(2683..2877)
                     /gene="rpsU"
                     /locus_tag="PKK81_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNV33261.1"
                     /translation="MEIKVFNNNVEKALKVAKKKLAGEGLFRELKRRRYYEKPSVRKK
                     AKEREAQRRRQKWLAKRRPE"
     gene            complement(2901..4463)
                     /gene="ftcD"
                     /locus_tag="PKK81_10035"
     CDS             complement(2901..4463)
                     /gene="ftcD"
                     /locus_tag="PKK81_10035"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNV33262.1"
                     /translation="MSQVVECVPNFSEGRNSEIVDALAGVVRSVPGVVLLDETKDPDH
                     HRAVVTFAGRPYAVAEVAYQMARMASQLIDLRNHHGEHPRVGATDVMPFVPIRGVSMQ
                     DCVQLARMVGQRIGNELKIPVFLYEQAATRPERKQLEWIRKGGLKGLADRMASDPAWV
                     PDFGPKLLHQTAGATVVGARWPLIAFNVNLKSRDLSVARAIAKLVRQSSGGLPYVKAI
                     GVELKSQQLVQVSMNLTNHEETPLHVVFAAVQREAAARGVEVAGTEIVGLVPEQALME
                     TAQQALCLNQFDGRQVLESRLDRPESREAIGRMAASQPVKEQSPLPAKVGAPDGKMPE
                     LQGQTGGSVGAQSAAFAAGLGMMVAKLTRARAIETRLSEIRTRLHELGQADRDAYGRV
                     LQARKLPITHPDRPLQLSSNLLGAIETPLEIVKLSCELIPLLRGLLTQTKPELQPDLK
                     MGLRLADAVIDGCLGMVEENMKVQPNQLLIASIRERFSVAEQMLVDAKSLCYTPPFDS
                     WAQNMLNILKLR"
     gene            complement(4603..4845)
                     /locus_tag="PKK81_10040"
     CDS             complement(4603..4845)
                     /locus_tag="PKK81_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNV33263.1"
                     /translation="MQADVKLDTLGYFCPMPIILTSKKIKELTTGQVLEVVSDDEGIK
                     KDMPAWCETTGHEMVGLEEEQSASKRIYKAFVKKTK"
     gene            complement(4869..5399)
                     /locus_tag="PKK81_10045"
     CDS             complement(4869..5399)
                     /locus_tag="PKK81_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNV33264.1"
                     /translation="MTTAQIEPTAALAELREAKPDRVTIVLLSGDLDKAMAAFIIATG
                     AAAMGMQVTVFFTFWGLNTIRKKGASSSAPDWLRRMFGLLNKGGAETLPLSRFHFWGL
                     GTKMMQVVMKQNRMPGVPELMETALELGVRFIACTTTMGLMGITKDTLIDGIDQFAGV
                     TTYLAEAKQGSVNLFI"
     gene            5644..6732
                     /locus_tag="PKK81_10050"
     CDS             5644..6732
                     /locus_tag="PKK81_10050"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNV33265.1"
                     /translation="MSTDLITELKPTKSAPLYGFWYPATTSTALAVGQMQTQVMLGQP
                     ILVCRDRQGKVAAMRDICPHRGMPLSFGHFDGERVECAYHGWQFDTGGRCRHIPALVE
                     GSPLQPEKIGITSYPCQDQDGYVWVFLPDPQQPKQPVPELPRLPLPSEPYRMIQISTI
                     LDCTIDDGIVGLMDPAHGPFVHQSSWWRTKASMHDKAKTFEPIPNGFRMVPHAPSKNS
                     GPYKLLNRLYGGPLTTTIDFVLPNQRFEFVQCGSMYVSSRATVTPVGEQQCRIDFAAA
                     WNILPWLPFAKPLFRYFANIFMKQDKQAMERQAVGLKYKPALMLIDDADTPAKWYYKL
                     KAAHLAAVQTGRPLDHPLKDRVTLRWRS"
     gene            complement(6742..>6993)
                     /locus_tag="PKK81_10055"
     CDS             complement(6742..>6993)
                     /locus_tag="PKK81_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNV33266.1"
                     /translation="VETCRIDDEAPAVGKSLTELSIRPRTGASVIAWTRSQVTQSNPS
                     ETVRLQAGDVVTLLGSRDQIRRAMALLNEPNHGTSHQLK"
BASE COUNT         1429 a         2187 c         1930 g         1447 t
ORIGIN      
        1 cctgcgaatc tgcttcacca aaccatttat ctttcacggg atcgtaaatg cgcgtcttgg
       61 gagtgagcag ttgaaacata tctttgtcgc tggtgacgat tacaacgtcg aggccttcaa
      121 cttcgccttt tctcgccagg gtgccgatca gatcgtcggc ctcgtatccg gcctgccgaa
      181 taaccggcag agataacgct tccaccacac gatggatgta gggaatctgc gcgctcatgc
      241 cttgcggcat aggtggccgt tgcgccttat agtctttgaa cgcttcatgg cggtgggtcg
      301 ggcccttttc atcgaacacc acggccacat agtccggccg atggtcccgc aggaccttca
      361 gcaacatcgt cgtgaagcca tacaccgcat tggtctgcag gcctttggaa ttggccaggg
      421 gaggcagggc gaagaatgcc cgatagatgt aggcgctgcc gtcgatgaga tagagtgtgg
      481 gcatagctga gataatgtaa ccggtggagc aacacgcagg agtcgaactt accgcagcgc
      541 tctcaatccg gtgcaatgat gagcggcggt ttgtcgaact tggcccgcat ggcgttgatg
      601 gtgctgagaa cggtggactg ccccacgatc ttggcctcca cccgccgctt gcctgggaat
      661 tcaatcaagg agaggccgat caacatcgtc agcacgccct gtccgggcag aaacagcatg
      721 agaaaaccgg cgaacagaaa aatcgcgccc acgacgttct tcaccacatg accaatcacg
      781 cgaaggatcg gatgatggtt ctccatccag ggtcgcggag tgcggacatc gaaatagtcc
      841 gccggcaacc gcatcaaaat gatgggaatc gcgatcaagg tccccacgaa catgaacacg
      901 gaggaaacgg cgaaccagat cagcacattc gccggcacgt aggattcgat ctgcgtcagg
      961 agttcgctta tcaccggcgc atcctagcat ggggctttgc atggcggaaa gggtggcgaa
     1021 gccaagccgg tttacgttgc ctgctccgcc agctataatt ccgcaccatg ccgccgatgc
     1081 gactcaacag cgcacgagtg acgagagcct gccttcaggc ctccggcctg gccgttctcg
     1141 gattcgccct ctcgttgtac cccgttgcgc tcagcagcca cgcagcttgg gcagctgatg
     1201 gcaccactga gtcggaactt cagatcgaca tcaccaagat cgggcttggt aaagaagtgg
     1261 tcattacaca aggtaagaag gaatggttca tgcgggtcga ggtcacaccg gaaaactccg
     1321 tggtggtgcg acaggaaaaa gaaggggagc gctacctcct cgacgagagc gagatgcacg
     1381 atcgcgccat gacacccgct gaagtagatg tagccatcgc cgactttgtg aatagcgtca
     1441 aaaccagaca gaaggtcaag tagctcccat gtcgggtaaa cccaaattcg tcatctttgc
     1501 ccacaacgcc acctacgaca aactgcacca ggtcgcgacc ctcggcctca ccgccgccgc
     1561 gatgggcaag gacgtcattg tggtgttgct gttctggaca attaagaaac tcgcagaagg
     1621 aaagatcgac gagatcgact ttccacccga atatgcggcc tcggcagaac aggtcgcccg
     1681 gcttctgaag gaaaagaagg tcccgaagat ctccgagatg ttccaggaag cacgtctggt
     1741 cgggcacttc cgcctcattg cctgcagcgc gggtctggaa tatatgggag tggcagcggg
     1801 cgcggtggaa cgcaacgtgg acgaagtgct tgggctcccc tccatcctct cgctcactgc
     1861 gcaggcggaa accaaattgt tcatctgaca tctctcgtcg ttcatatctc actaaagggc
     1921 tggtggcttg aagcgtatcg gcgctccacg cttaacggct tttcgtcacg ccgacgcgat
     1981 cgcaatacgc cgcaatcttg cactggctgc acaatggcga gaccggctgg cagaggtgtt
     2041 gtccgtaggg cacgagcaga tcgttgaatg tgatccaata tttgcgcgga agtttctcgc
     2101 ggagagctgt ttctgtctca tcgggactct tggtcttgac gtacccccat cggttgctga
     2161 tccggtggac gtgaatatcg acgcaaatcc ccggcttttc atacccgacc gtcaccacca
     2221 gattggccgt cttcctcccg actcccggca acgtcagcaa ttcatcgatc ttgtccggca
     2281 cccgaccctg gtaccgctca cggagttggg cgcagatctg ctgaatctgt ttggcctttg
     2341 tccgatagaa cccgacggga tagatggcgc gctcaatgac cggggtggtc agcgtctgca
     2401 tggtggccgg tgtggacgcc aaggcgaaga gccgctcgct ggcttcggcc gtggtcttgt
     2461 ctttggtgcg tagactgagg agacaggaaa tgagcacgag gaagggatca cggcctgact
     2521 gtctggcaac cacgccgaca accgggtctg gccagcgagc gatttcccgc ttgaccgttc
     2581 ggatggcggc gtggatctct tgcgcctgca tggagctgcc ggagaggaga actcaacagg
     2641 gagtggtcga actagtcggc tgtggaaacg aacgcctgac gtctattcgg gccgtctctt
     2701 tgcgagccac ttctgccggc ggcgctgcgc ttctcgctct ttcgccttct tccgaacgct
     2761 cggcttttca taatagcggc ggcgcttcag ttcgcggaac aatccttcgc ccgccaattt
     2821 cttcttggcg accttcaggg ctttttcgac gttgttattg aaaaccttaa tttccatcgt
     2881 cagctacctt cgactctctt tcaccgaagt ttcaggatat tcaacatgtt ttgggcccac
     2941 gaatcgaagg gcggagtgta gcatagcgat ttcgcatcca caagcatttg ttccgcaacg
     3001 gagaatcgct ctcgaatcga ggcaataagc agctgatttg gttgaacttt catgttctcc
     3061 tcgaccattc cgaggcaccc atcgatgacg gcatcggcaa gacgaaggcc catcttcaga
     3121 tccggctgaa gctccggttt cgtttgcgtc aggaggcccc gcagcaaggg aatgagttcg
     3181 caagacagct tcacaatttc caacggcgtt tctatcgccc ccagcaagtt cgatgagagt
     3241 tggagcggac ggtcaggatg ggtgatcgga agctttctcg cctggagcac ccgcccatag
     3301 gcatctcgat ccgcttggcc caattcgtgg aggcgtgtgc gaatttcgga taagcgcgtt
     3361 tcaatcgcac gcgcgcgagt cagcttcgcc accatcattc ccaacccggc cgcaaacgcc
     3421 gccgactggg cccccacgct gccgccggtc tgcccctgaa gctccggcat cttcccgtcc
     3481 ggcgccccga ccttcgcggg caacggcgac tgctccttca ccggctgcga cgccgccatc
     3541 cgcccgatcg cctcccgcga ttcgggacgg tccaatcgcg actcaagcac ctgtcgtccg
     3601 tcgaattggt tgagacagag cgcctgctgc gccgtctcca tgagcgcctg ttcgggaacc
     3661 aacccgacga tctctgtgcc ggccacctcc acaccacgag ccgccgcttc ccgctgcacc
     3721 gccgcaaaca cgacatggag cggcgtctct tcatggttcg tcaaattcat cgatacctgc
     3781 acgagttgtt ggcttttcag ctccacgccg atcgctttga cgtacgggag cccgccgctt
     3841 gattggcgca ccaatttggc gatggccctg gcaaccgaaa gatcgcggct cttcaaattc
     3901 acgttgaagg cgatcagcgg ccatcgcgcc cccaccaccg tggccccggc cgtctggtgc
     3961 aggagcttcg ggccgaaatc cggcacccac gcagggtcgc ttgccatccg atccgccagt
     4021 cccttcaagc cacccttccg aatccactcc agctgtttcc gctcgggcct ggtcgccgcc
     4081 tgttcgtaca gaaagaccgg gatcttcaat tcattcccga tacgctggcc gaccatgcga
     4141 gccagttgca cacaatcctg catgctgacg ccacgaatcg gcacaaacgg catcacatcc
     4201 gtcgccccga cgcgcggatg ttcgccatga tggtttcgga gatcgatcaa ctgggaggcc
     4261 atccgcgcca tctgataggc cacctcggcc acggcatagg gcctaccggc aaaggttacg
     4321 acggcccggt gatggtccgg atccttcgtt tcatccagca ggacaacgcc gggtactgag
     4381 cggaccactc cggctaacgc gtcgacgatc tcggagttcc gtccctcgct gaagttcggg
     4441 acacattcaa caacctgact cacgaggttc cctctctcac ccgacccggt ccgccccact
     4501 taccctggaa gtctgaggca acaaaaaagc cggaggctgg tgatcatgcc ctccggcttt
     4561 gcctaacagg acggccccgc gttcgcgcgc ctgtcgctgc ccctacttgg ttttcttgac
     4621 gaaggcctta tagatccgct tggacgcact ctgctcctct tcgagcccca ccatttcgtg
     4681 gccggtcgtc tcacaccagg ccggcatatc cttcttgatg ccttcatcgt ccgacacgac
     4741 ttccagcacc tgaccggtcg tcagttcctt gattttcttg gacgtcagaa taatcggcat
     4801 cgggcaaaaa tacccgagcg tatcgagttt cacatcagcc tgcatcgtgc cgctcctcaa
     4861 cccctcgctt agataaacag attgacgctg ccctgcttgg cttcggccag gtaggtcgtc
     4921 acaccggcaa attggtcgat tccgtcgatc aacgtatctt tcgtgatgcc catcaggccc
     4981 attgtcgtcg tgcaggcgat gaagcgaaca ccgagctcca gcgccgtctc catgagttcc
     5041 ggcacacccg gcatacgatt ctgcttcatc accacttgca tcatcttcgt gcccagcccc
     5101 cagaaatgga agcgggacag cggcaacgtc tcggcgccgc ccttgttcag taaaccaaac
     5161 atccggcgca accaatccgg ggccgaactg cttgcccctt tcttgcgaat cgtgttgagc
     5221 ccccagaatg taaagaagac cgtcacctgc atccccatgg cggccgcacc ggtggcgata
     5281 atgaaggcgg ccatggcctt atcgagatct ccgctcagca acacaatcgt cacacgatcc
     5341 ggtttcgcct cgcggagttc cgccagtgca gcggtgggct cgatttgtgc ggttgtcatg
     5401 agggtacctc gtctctcgga gtagggatat ggagtcacat acgatggaaa ttgactatag
     5461 tctccgcttc tttttcttgt caagaaatgc gggtcagaag cggggtttgg ccatccagat
     5521 cgtaccgtct gcggccttcg ccctagactg ccggttgctt gacctgttgc gggcccgccg
     5581 ttatagtcca ttcggcggcg aaccgtctca cgcatcgcga caaacccggc gactgcatca
     5641 ctcatgagca cagacctcat caccgaactc aaaccgacaa agagcgcccc gctctacggc
     5701 ttctggtatc cggcgaccac gagcacggct ctggctgtcg gccagatgca aacccaggtg
     5761 atgttaggac aaccgatcct cgtctgccgt gaccgccaag gcaaggtggc ggcgatgcgc
     5821 gacatatgcc cccatcgcgg catgccgctc tcgttcggac actttgatgg agagcgcgtc
     5881 gaatgcgcct atcacggatg gcagttcgat accggtggcc gctgccgcca tattccggca
     5941 ctcgtggaag ggtccccgct ccaacccgaa aagatcggca tcacgtccta tccctgccag
     6001 gatcaggatg ggtacgtgtg ggtgtttctc ccggatccgc aacagccgaa acaaccggtt
     6061 ccggaactcc cgcgtctccc gctcccgtcc gaaccctatc gcatgatcca gatttcgacg
     6121 attctggact gcaccatcga cgacggcatc gtcggactca tggacccggc ccacggtccc
     6181 tttgtgcacc aaagttcctg gtggcggacc aaagccagca tgcacgacaa agcgaagacc
     6241 ttcgagccga tccccaacgg gtttcgcatg gttccccatg cgccgtcgaa gaacagcgga
     6301 ccctacaagc tcctcaaccg gctctacggc gggccgttga cgacgactat cgacttcgta
     6361 ctccccaatc aacgcttcga gttcgtacaa tgcggttcga tgtacgtctc gtcgcgcgcc
     6421 acagtcacgc ccgtgggtga gcaacaatgc cggatcgatt ttgctgcggc ctggaacatc
     6481 cttccttggc tgccctttgc caagccgctg ttccggtatt tcgccaatat cttcatgaag
     6541 caggacaagc aggcgatgga gcggcaagcc gtgggactga aatacaaacc ggcgctcatg
     6601 ctgatcgatg acgccgatac gcccgccaag tggtactaca aactgaaggc cgcccatctc
     6661 gccgccgtcc aaaccggacg tcccttagac catccgctga aagaccgtgt gacgcttcgc
     6721 tggagaagtt agccgaccgg gttatttgag ctggtgactc gtgccgtggt taggctcgtt
     6781 caagagcgcc atggcccgac gaatctggtc gcgggatccg agcagcgtga cgacatcgcc
     6841 ggcctgcagc cgcacggttt cggagggatt cgactgagtg acctggctgc gagtccaggc
     6901 aatcaccgac gcgccggtcc gtgggcgaat ggagagttcc gtcagcgact ttcccaccgc
     6961 cggagcctcg tcatcgatcc ggcaggtttc cac
//
LOCUS       DAOQRM010000129         6970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10640_length_6970_cov_12.454519, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000129 DAOQRM010000000
VERSION     DAOQRM010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10640_length_6970_cov_12.454519"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..2001
                     /locus_tag="PKK81_10060"
     CDS             325..2001
                     /locus_tag="PKK81_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNV33267.1"
                     /translation="MQYIARLVLGVALFAGPVVSGEVRAEGPVLHPNAAVPGSTLSIT
                     GKGFGPYKSTKFNQVTFQGVPALIQRWEADLIEVRVPSQAANGPVDVIIGKKHVKAGS
                     FTRLQPAIHSLSPAEAEPGTILEITGEHFGNTAGPRDPNTIFGVNTVAVGDVTVRVRK
                     WRDDKIEVELPGNVQSGDVVIRMASSDPLPDGSCCAPVKQVVSNSMPMKVLASVRVDP
                     TSGPVGTKVVLFGKGFGTSRNPEDGVLFGGHLATVSQWTDTTIVVHVPLDAQTGPVVM
                     KRNGQERAIGTYTVQTPQATGLTPAEAPIGTLLKITGENFGFYSEAGSTPFNYIDFSL
                     SENTVEIGGVQAIVYRWGHDRIDVWVPFSAKSGPVVVKRAANAPKPDGTCCADKKVLE
                     TQVGNFTLVTPKIDSYSPTTGGLDEVVTIKGSGFGKFLKTAEPSKLITDSVYARVAPV
                     LGENVSRTEVLFNGVGAIVQSWTDTEITVKIPHRHSYGVGKLGEFNPDLTSGPLVVRR
                     GSWDLLPDGSCCTPKKWLTLEAGTFTIQPTGLPDASYWRNQSSENTHHQQ"
     gene            2013..>2355
                     /locus_tag="PKK81_10065"
     CDS             2013..>2355
                     /locus_tag="PKK81_10065"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNV33268.1"
                     /translation="MHILAVVSWLFIALLCAGTAEAASPESRATVAMQKSGSEATLLG
                     MFFHDPQLGWAVGSGGTILKTTDGGRKWKKLSSGTTSLLTAVYFQDARRGWVVGANGT
                     VRTSRDGGETWS"
     assembly_gap    2356..2455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2456..2704
                     /locus_tag="PKK81_10070"
     CDS             <2456..2704
                     /locus_tag="PKK81_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33269.1"
                     /translation="LPCGRTCNKLTDLIRVRFIDAQQGWIVGEHGQILRTTDAGFNWV
                     EEASPVKRSLMALSFPHTTAGWAAGEGGTIISISPGRR"
     gene            complement(2724..3461)
                     /locus_tag="PKK81_10075"
     CDS             complement(2724..3461)
                     /locus_tag="PKK81_10075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNV33270.1"
                     /translation="MPAFFVSSLDIRETQITLTGDLCHHVRASLRVKPGEDLWLTDEQ
                     RRRYRVRVTHVSQQAVMTEILEQRLGPVETGPPLLLAQALLKGDHMDWVVQKASELGV
                     RTILPLISRHGVVRPQPDRIAAQVARWQRIATEAAQQSEQWQPPEVLEPLESRRFFST
                     HKATCSLLLAERHEAAALARIPLPSLPSEQLTVMIGPEGGWAEEEITLALAQHCQAVS
                     LGSCILRADTAAVTALSIVQSRLGRLG"
     gene            complement(3496..4608)
                     /gene="dnaJ"
                     /locus_tag="PKK81_10080"
     CDS             complement(3496..4608)
                     /gene="dnaJ"
                     /locus_tag="PKK81_10080"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNV33271.1"
                     /translation="MAKRDYYETLGIERTASDDEIKKAFRKLARQHHPDLHTSPEQKK
                     SAEEKFKELNEAYEVISDQEKRRRYDAFGHAGGPQGAEGFDFGGQGGFGDIFNDIFED
                     FFGQPRGRARAERGNDLQYNLEVTFEESVFGKEAKLKIPRWEICADCKGTGARSATAI
                     KMCPACKGQGQLRFQQGFFSVSRPCGQCEGVGQIITEPCQACGGRQRIRKERMLSVQI
                     PGGIETGMRLRLANEGEHGVQGGPQGDLYVAITVKPHPHFRREGQDIVSELPVNLVTA
                     ILGGRVEVPTLKGNTFMKIPAGTQPDKILRIKGLGFPNLKGGHTGDQLFNVKVEIPTK
                     LNSKQKELLEEFAKESGYTKDTEGEGFLDKMKTFFE"
     gene            complement(4690..6621)
                     /gene="dnaK"
                     /locus_tag="PKK81_10085"
     CDS             complement(4690..6621)
                     /gene="dnaK"
                     /locus_tag="PKK81_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNV33272.1"
                     /translation="MGKVIGIDLGTTNSCVAIMSGGDPVVIANAEGSRTTPSVVGITD
                     KNERLVGQIAKRQAITNPENTIFSVKRLMGRKFRSKEVQEAMKRLPYKVVEADNGDAH
                     VELRGKRYSPPEVSAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDVSVLEIGDGVFEVKSTNG
                     DTYLGGDDFDERVMDWLVEEFKKDQGIDLRKDRMALQRLKEAAERAKIELSSSQESEI
                     NLPFITADASGPKHMVTKLTRAKLEQLVGDLIQRTIEPCRKALADAGVSAKDIQEVVL
                     VGGMTRMPKVIQVVKEFFGKEPHRGVNPDEVVAIGAGVQGGVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTIPTKKSQVFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGIPPAPRGMPQVEVSFDIDANGIVHVAAKDLATQKEQSIKITASSGLSKDEVD
                     KLVKDAQSHTEDDKKRRRVAEARNQADSLLYSTEKNLSEHGDKIGEDDKTKITEAIAG
                     LRKAMEGDDPAAIETATQTLTTASHKLAEEMYKKASAAAGAGAGADAAASSGDGGAQA
                     KTDEKVVDAEFEEVDKDKK"
     gene            complement(6711..>6970)
                     /locus_tag="PKK81_10090"
     CDS             complement(6711..>6970)
                     /locus_tag="PKK81_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNV33273.1"
                     /translation="GSVDALTEGVELTLKQLVGALTKFGVKAVESVGLAFDPATQQAV
                     AQVPSDTIPENHVVEEYQKGYLLQDRILRAAMVTVSTGAAN"
BASE COUNT         1203 a         1986 c         2095 g         1586 t
ORIGIN      
        1 gctcgcgtgg cgctgggtgc ggcagagttg cccttgcacg ctgcaattga actcgaattg
       61 ctggtccagg ttcgaccata aaacactttg ctccgtctgt tcccttcctg tcgcgcccgc
      121 ttgccttgac atcaaaaaaa tccgcttgtt atagtccgta cgccccttcg ttcattgccg
      181 taccccccat ccggcctcgc tgcctcagtt gcgtggggtt ttcgtgccac gtgcggggcg
      241 ttcgtctgat gtgtcatcat ttcagcctcg cccgagcctg ctggtcaggc ctgccccgag
      301 gctttcggta gggagtgtca tcccatgcaa tacatcgctc gtctcgtgct cggtgtcgcg
      361 ctgttcgcag gcccggtcgt ttccggagaa gtccgggcag aggggccggt tcttcatccc
      421 aatgctgccg tgccgggatc gaccctctcg atcaccggga aggggttcgg cccctataaa
      481 tcgacgaagt ttaatcaggt caccttccag ggcgtgccgg ccttgatcca acgctgggag
      541 gccgatctca tcgaagtgcg cgtcccttcg caggcggcca atggaccagt cgacgtgatc
      601 atcgggaaga agcacgtaaa ggccggatcg tttacgcgat tgcagcccgc cattcattct
      661 ttgtcgccgg ctgaggccga accgggaacg attctggaga tcacgggcga acatttcggc
      721 aacactgccg gcccgcgcga tccgaacacg atttttggcg tcaatactgt ggccgttggc
      781 gatgtgacgg tgcgtgtccg taagtggcgt gacgacaaga ttgaagtgga gctgccgggt
      841 aatgtgcagt cgggtgatgt ggtgattcgc atggcttcgt ccgatccgtt gccggacggg
      901 tcttgctgtg cgccggtcaa gcaggtggtg agcaattcga tgccgatgaa ggtgctggcg
      961 tcggtgaggg tcgatcctac gagtgggccg gtcggaacga aggtggtgct gttcggcaaa
     1021 gggttcggga cgtccaggaa ccctgaggat ggcgtgctct ttggcgggca tctggccacc
     1081 gtgtcgcagt ggacggatac gaccattgtg gtgcatgtgc cgctcgatgc ccaaaccggc
     1141 ccggttgtga tgaagcgcaa tgggcaggaa cgggccatcg ggacgtacac tgtacagacg
     1201 ccccaggcca ccgggctgac tccggccgag gcacctatcg gcaccttgtt gaaaatcacg
     1261 ggagagaatt ttggatttta ttcggaggcc gggtctacgc cctttaacta tatcgatttt
     1321 tcgttgagtg aaaacactgt cgagatcggc ggggtgcagg cgatcgtgta tcgatggggc
     1381 cacgaccgca ttgatgtctg ggtgcccttc agcgcgaaaa gtggtccggt ggtggtcaaa
     1441 cgtgctgcca atgctcccaa gccggatggc acgtgctgcg ccgataagaa ggtgcttgaa
     1501 acgcaggtcg gcaattttac gctcgtcacg ccgaagatcg attcctacag tccgaccacc
     1561 gggggattgg atgaggtggt gacgatcaag gggagcgggt tcgggaagtt tctcaagacg
     1621 gccgaaccaa gcaagctaat cacggatagc gtctatgccc gggttgctcc cgtgctggga
     1681 gagaacgtat cccgcaccga agtgttgttc aacggagtcg gggcgatcgt gcagtcctgg
     1741 acggacacgg aaatcacagt caagattcct catcgccatt cctatggcgt gggcaaactc
     1801 ggcgagttta atccggacct gacgtccggg ccgctcgtgg tccgtcgtgg atcgtgggat
     1861 ctcctgccgg acggctcctg ttgcacccca aagaagtggc ttacgctcga ggccggcacg
     1921 tttacgatcc agcctaccgg actgcctgat gcgagttact ggcggaatca aagttcagag
     1981 aatacccacc accaacaatg aggcgtcgta cgttgcatat cctggcagtt gtatcgtggt
     2041 tgttcatcgc cctcctgtgc gcaggcacgg ccgaggcggc ttccccggag agccgggcta
     2101 ccgtagctat gcagaaaagt gggtctgaag ccacactgct gggcatgttc tttcacgatc
     2161 ctcaacttgg ctgggccgtg gggtccggcg gaacgattct caagacaacc gacggcggcc
     2221 gcaaatggaa gaagctctcc agcggcacca cgtcgctgct gacggccgtg tatttccagg
     2281 atgctcgtcg aggatgggtc gtgggcgcca atggcaccgt gcggaccagc cgggatggtg
     2341 gagaaacctg gagcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttacc
     2461 ctgcggccgg acctgtaata aactcacgga tctgattcgt gttcgtttta tcgacgcgca
     2521 gcaaggctgg atcgtgggag agcatgggca gattttgcga accaccgatg caggatttaa
     2581 ttgggtggaa gaagccagcc ccgtgaaacg ctcgctcatg gccttgagtt ttcctcacac
     2641 caccgccggg tgggcggcag gcgagggggg taccattatt tcgatcagcc caggccgtcg
     2701 gtagcgctcg ccagatcccg atcctacccc agtcggccca atcgactttg cacaatactc
     2761 agtgcagtaa ctgctgcggt gtccgcacgg aggatacacg agccgagact cacagcctgg
     2821 cagtgctgag ccagggccag agtgatctct tcttctgccc atcctccttc aggtccgatc
     2881 atcacggtga gctgttccga ggggagactc ggcaggggta tcctggccaa tgcggcagcc
     2941 tcatggcgtt ctgccaggag aagcgagcag gtcgctttgt gagtggagaa gaagcggcgt
     3001 gactccagcg gttccaacac ttccgggggc tgccactgct ccgattgttg cgccgcttcg
     3061 gtggcgatgc gctgccagcg ggccacttgg gcggcgatcc tgtccggctg cggtcgtacg
     3121 accccatgcc gtgagatgag gggaaggatc gtccgcacgc ccaactcgct cgccttttgc
     3181 accacccaat ccatatggtc gcctttgagc agggcttgtg cgagcagcag gggggggccg
     3241 gtctcgacgg gtcccagccg ttgttcgaga atctccgtca tcaccgcctg ctgggatacg
     3301 tgcgtcaccc gcacacgata tcgtcgccgt tgttcatcgg tgagccagag atcttcgccg
     3361 ggtttgacgc gtaggctggc ccgcacatga tggcagagat cgcccgtgag ggtgatctgg
     3421 gtttcacgaa tatctaatga ggagacgaag aaagccggca tggtggtcgg cggaacaggt
     3481 agggcggtga tcggtctatt cgaagaacgt tttcatcttg tcgagaaagc cttcgccctc
     3541 ggtgtctttc gtgtagccgc tttccttggc aaattcttca agcagctctt tttgctttga
     3601 attgagtttg gtcggaatct cgactttcac gttgaacagc tgatcgccgg tatggccgcc
     3661 cttgaggttt ggaaagccga ggcctttgat gcggaggatt ttgtccggct gcgtgccggc
     3721 agggatcttc atgaaggtgt tgcctttgag cgtcggcacc tccactcgcc cacccaggat
     3781 ggcggtgacg agattgacgg ggagttcgga cacgatatcc tggccttccc gcctaaaatg
     3841 aggatggggc ttcacggtga tggcaacata caggtccccc tgagggccgc cctggacgcc
     3901 gtgttcgccc tcgttcgcga gacgcaggcg catgccggtt tcgatcccgc cgggaatttg
     3961 cacggagagc atgcgttcct tgcggatacg ctgccgcccg ccgcaggcct ggcagggttc
     4021 ggtgatgatc tgtccgaccc cttcgcattg accgcagggc cggctgacgc tgaaaaaacc
     4081 ctgttggaac cggagttggc cctgtccctt gcaggccggg cacattttaa tggccgtggc
     4141 tgagcgcgct ccggtgcctt tgcaatcggc acagatttcc cagcggggga ttttgagttt
     4201 cgcttccttt ccgaataccg actcttcaaa cgtcacctca aggttgtatt gcaggtcgtt
     4261 gccgcgctcg gcgcgggctc gtccgcgagg ctggccgaag aagtcttcga aaatatcgtt
     4321 gaagatgtcg ccgaatccgc cctgtccgcc gaaatcgaat ccctccgctc cctgcggtcc
     4381 accggcatgg ccgaaggcat cgtatcgccg ccgtttttcc tggtcgctga tgacctcgta
     4441 ggcttcgttc agctctttga atttttcttc cgcggatttt ttctgttccg gcgatgtgtg
     4501 gagatcgggg tggtgctggc gggcaagttt ccggaatgcc ttcttgatct cgtcgtcgga
     4561 agcggttcgc tcaatgccga gagtttcgta gtagtcgcgc ttagccacgg aagattctta
     4621 cccggttaaa tgtgcaacac gaccgagctt cacgcatggt gaaactcggt cgtgcaatcg
     4681 gtgagggtct tactttttgt ctttgtcgac ttcttcgaac tccgcgtcca cgaccttttc
     4741 atcggtcttg gcctgcgcgc cgccgtctcc ggatgaagca gctgcatcag ccccggcgcc
     4801 ggcaccggct gcggcagagg ctttcttgta catctcctca gccagcttat gggatgccgt
     4861 ggtcagcgtt tgcgtggccg tttcgatggc tgcgggatcg tcgccttcca tcgccttacg
     4921 gagtccggcg atggcttcgg tgatcttggt cttatcgtct tcgccgatct tatcgccatg
     4981 ctcgctcagg ttcttttcgg tgctgtacag caacgagtcg gcctggttac gcgcttcagc
     5041 cacccgacga cgtttcttgt catcctcggt gtgggactgg gcgtccttga cgagcttatc
     5101 gacttcgtcc ttgctgagtc cgctcgaggc cgtaatcttg atggactgtt ccttctgtgt
     5161 ggccaggtct ttggccgcca catgcacgat accgttggca tcgatgtcga aggagacttc
     5221 gacctgcggc atgccgcgcg gagcgggagg aatgccgacg agatcgaatt gtcccaacag
     5281 cttattgtcg ttcgccattt cacgttcgcc ctggaatacg cgaatggtga cggctgtctg
     5341 gttgtcggca gcggtcgaga atacctggct cttcttcgtg ggaatcgtgg tgtttcgctc
     5401 gatcaacttc gtaaagacgc cgccgagggt ctcaatgccg agcgacaagg gcgtgacatc
     5461 cagcaacagc acgtccttga cttcgccctt aagcacgccg ccctgaacac cggctccgat
     5521 ggcgacgact tcgtcggggt tcacgccgcg atgcggttct ttgccgaaga attccttcac
     5581 gacctggatg actttcggca tgcgggtcat gccgccgacg agcacgactt cctggatgtc
     5641 tttcgcgctc acaccggcat ccgccaaagc cttccggcag ggctcgatgg tccgttggat
     5701 gaggtcgccg acgagttgtt ccagctttgc gcgggtcaac ttcgtgacca tgtgcttcgg
     5761 gccgctggcg tccgccgtaa tgaacggcag gttgatctcg ctttcctgag acgaggagag
     5821 ttcgatcttg gcccgctccg ccgcttcctt gaggcgttgc aacgccatcc ggtctttgcg
     5881 caggtcgata ccttgatctt tcttaaactc ctcgacgagc cagtccatca cacgctcgtc
     5941 gaagtcgtcg ccaccgagat aggtgtcgcc gttcgtggac ttgacctcga acacgccgtc
     6001 gccgatttcc aggacggaca cgtcgaacgt cccgccgccg agatcgtaga cggcaatgcg
     6061 ctcgtctttt ttcttgtcga gaccgtaggc cagtgaggcc gccgtgggtt cgttgatgat
     6121 gcgcaggacg ttcaatccgg cgatctgccc cgcatccttg gtggcctggc gctggctatc
     6181 atcgaaatag gccgggaccg tcaccactgc ttcagtgacc ttctcaccca ggtaatcttc
     6241 ggcggtctgc cgcatcttct gcagaatcat ggcggagact tccggcgggc tgtaacgctt
     6301 tccgcgcaac tcgacgtgcg catcgccgtt gtccgcctcc accaccttgt aggggagccg
     6361 cttcatggct tcctgcactt ctttgctgcg gaacttgcgt cccatcaggc gcttgacgga
     6421 gaagatggtg ttttccggat tggtgatggc ttgccgcttg gcgatttgtc cgaccaggcg
     6481 ctcgttctta tccgtgatgc ccaccaccga cggagttgtg cgacttcctt ccgcattggc
     6541 gatgacgacc gggtctccac cgctcatgat agcgacgcag gagttcgtcg tgccgaggtc
     6601 gattccgata actttgccca tgggttggct ccttcgcaaa aaatatcaat acatgaacgg
     6661 tcgtgtcagt tgtgcttgcg aaacctgttc ttcacaccga tgattcggcc ctagtttgcg
     6721 gctccggtcg acacggtcac catggcggcg cggaggatgc ggtcctggag gagatatccc
     6781 ttttgatact cttccacgac atggttttca gggatcgtgt ctgacggaac ctgggcgact
     6841 gcctgttgcg tggctgggtc aaaggccagg ccgacgctct ccactgcttt gacaccgaac
     6901 ttcgtcagcg cgccaacgag ttgcttcagc gtcaattcca caccctctgt caacgcatcg
     6961 acggagccgc
//
LOCUS       DAOQRM010000130         6957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10673_length_6957_cov_15.641263, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000130 DAOQRM010000000
VERSION     DAOQRM010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10673_length_6957_cov_15.641263"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKK81_10095"
     CDS             <1..371
                     /locus_tag="PKK81_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNV33274.1"
                     /translation="GTCVDIVTQAAIQKMMIPGLIPVVAPILVGVILGPQALGGVLVG
                     SIVTGLFVAISMTSGGGAWDNAKKYIEEQGLKGTDTHKAAVTGDTVGDPYKDTAGPAI
                     NPMIKVINIVALLIVSLIVK"
     gene            535..732
                     /locus_tag="PKK81_10100"
     CDS             535..732
                     /locus_tag="PKK81_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HNV33275.1"
                     /translation="MEKQERRPESRKDSHQKEEVKPNPKVVESGKKMKEDIDKLVDEI
                     DDVLEKNAEEFVKNYVQKGGE"
     gene            941..2647
                     /gene="recJ"
                     /locus_tag="PKK81_10105"
     CDS             941..2647
                     /gene="recJ"
                     /locus_tag="PKK81_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNV33276.1"
                     /translation="MQEKLWVFRPSDIALQNNLSRQLSISPVTASVLLARGVTTPDEA
                     TRWMSGVHGGLHDPFLLPDMEPAVERLHQALARQERVCFYGDYDVDGVSATSLYLSFY
                     QGLGGNACGYIPHRVREGYGLNEGAVRRLAEEGILLLVTSDCGTTSHHEIAVARELGM
                     DVIVTDHHQTDATMPAALAVLNPHRRDAIYPFKGLCSAGLAYKVVSAYQQRYGSGDVE
                     PDSCLDLVALATVADIVPLQDENRILVREGLTHITRGARAGIRALKQVAGIAKACTSE
                     TIGFRLGPRLNAAGRLDHAMLCVRLLTTESDQEAMQIAEQLEQLNRQRQQIEEGITGE
                     AATYLQQDEPAAAIVLGSRDWHLGVVGIVAARLVDRFHRPSIVMAIDGQGIGKGSART
                     VEGFDLYQGLSECKDLLEAYGGHPSAAGLTLKESRLEEFRRRFCEVVAQWAGSARTVP
                     TLHVDAEVKLTDVNFDLIQELESLHPFGAGNPEPTLAVRGLDVVDARVVGEKHLKLRV
                     RQGRSFIFDSIGFRMGSFEELGLRAGRPVDLAFSPERNHWNGYDRVQLRIKALRMSGE
                     VS"
     gene            2650..4833
                     /locus_tag="PKK81_10110"
     CDS             2650..4833
                     /locus_tag="PKK81_10110"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNV33277.1"
                     /translation="MPHETVTELSQLIDRVKSYNVEADLDLVRRAYDFSAKAHEGQMR
                     RSGEPYFRHPLAVAGLLTHLKTDVTAIVAGLLHDTLEDTLATPLELEQRFGKDVVHLV
                     DGVTKIGKITFRNYEEKQAENFRKMVLSMADDIRVVLIKLADRLHNMRTLEYLSEGKR
                     QQIAQETLEIYAPLANRLGIGWIKNELEDLCLKHLKPDVYESLRVRVAKRDEDRQQYI
                     IEVIDLVKKAMAEAGLQGEVSGRPKHLYGIYQKMEKQSISFEEVYDLAALRIITDIKM
                     NCYALLGVIHSLWRPLPGRFKDYIAIPKSNMYQSLHTTVVGPQGEHVEFQIRTEEMHR
                     VSEQGIAAHWKYKEHGRIDEKDGKVFSWLRQFVEWNQDLPDNRQFMDSVKLDLFHDVV
                     YVFTPQGMVKELPKGSTPVDFAYSVHTEIGDHCVGAKVNGKIVPLKHMMESGDMVEIL
                     TAANQTPHRDWLKFVRTSRAKTKIKHWIKAEEQSRSIEIGKRLLEAEFRRHGLAPAQM
                     MRSEQLLAVAKQVGYETPEELAAAVGFGHVPTSQVMSKINPPVATEGQTVAPEVPPPP
                     KPVTGRTDDTGVQVKGARDLLMQLSRCCNPVPGDRILGYITRGRGLTIHTVDCPNLEA
                     LDYDKNRLVEVEWDHSTPSTHSVKVSVIAVDKTGVLAHVSTAISECQANISRAEITTR
                     EDRKAMLDFIIEVLDTAHLARVLKAIERVDGIITVRRVRSWQERS"
     assembly_gap    4890..4989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4990..6249
                     /locus_tag="PKK81_10115"
     CDS             <4990..6249
                     /locus_tag="PKK81_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNV33278.1"
                     /translation="TILDALIIGEWLIERGQRYIPDIVRQLLIGAEVVAAGVLILWLV
                     MGINLVALVALPTVAAAMVGVALKDTLTRFFSGIELGKIVKVGEWITVVDREGMVTHI
                     GMEHVTLLTRAQDLVSLPNDLVIQSGVTNFSRPTTTHFCNVYVDAAYRTPPEQVCATL
                     LEAAVAVPGVLQDPKATALVTAFNESAIQYRIKFPIGDFAQRDMIESRMRTYIWHAFA
                     RNGIEIPFPQRVVHQTVEPEVVTNGQAVGRMVAQLAAVDFLGTLDAKQLEALAKEARW
                     ESYLPGERVVRQGEPGEVLYVIASGKADVRLEQGGLSSTVTTLEAGKFFGEMSLLTGE
                     PRSATVSAATELLVIAVGKQSLLQVVQEDRRLIERIGEVVARRQAATAAAKAQLSREA
                     AALSAVTHTRSLVERIQNFFWGARKAQ"
     gene            complement(6266..6715)
                     /locus_tag="PKK81_10120"
     CDS             complement(6266..6715)
                     /locus_tag="PKK81_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNV33279.1"
                     /translation="MWCLTGITMLSMTCFSLAADPQADTLITVKTPTGTLIQAELADT
                     ALKRAQGLMFREHLADDRGMLFIFGDAQPWTFWMKNTKIPLDIIWMDAKKTIVHIERN
                     VPICTRQDDGCPQYHSDEGALYVLELGGGRAAALQLQRGMKLSFKQP"
BASE COUNT         1391 a         1780 c         2222 g         1464 t
ORIGIN      
        1 acggcacctg cgtggatatc gtgacgcagg cggcgattca gaagatgatg attccggggt
       61 tgattcccgt cgtggccccg attctggtgg gcgtcatcct tggaccacag gcgcttggcg
      121 gagtgctggt ggggagcatc gtgacgggtc tctttgtggc catctccatg acgagcggcg
      181 gcggcgcctg ggacaatgcc aagaagtaca tcgaagagca gggcctgaag gggaccgata
      241 cgcataaggc ggcggtgacc ggggacacgg tcggcgaccc gtacaaggat acggccgggc
      301 cggcgatcaa tccgatgatc aaagtcatca acatcgtggc gctgctgatc gtctcgctca
      361 ttgtgaaata gctgcgggtg tgaggtgggg ttggtcgctc gtgaggcgtt ccaggcgtta
      421 tgcgagcgac tccgtacaca atgtgctcct tggcttgacg cgttcgacga ccctagagta
      481 aggtgacaag gagtgggtag ttcgaacagg ttctgattga ctggaggtgc tccgatggag
      541 aaacaggaga ggagaccgga atccaggaaa gactctcatc agaaggaaga ggtcaagccg
      601 aatccaaaag tcgtcgagtc cgggaaaaaa atgaaagagg acatcgacaa gttggtcgat
      661 gaaatcgacg acgtgctcga aaaaaatgcc gaagagttcg tgaagaacta tgtgcaaaag
      721 ggaggggagt agtcccctcc ctttttgtta tccgctcccg gtccttccct cctttttgtg
      781 ccacgacgct tcgtccgttc atcagagggc tgatcgtgac ccgacgctgc gtctcgtcct
      841 atccggcgcg agaacccgtt tgacaaaaaa atggtgagtc gataccattc cccttacttc
      901 tcagtaactt ctgaatattt caggcaaaga cagaggattc atgcaggaaa aactgtgggt
      961 gtttcgaccg tctgatatcg ccttgcagaa taatttgtcg cggcagctct cgatctcgcc
     1021 ggtgacggcg tcggtgctgc ttgcgcgcgg tgtcacgact cccgatgaag cgacccgttg
     1081 gatgtccggc gtgcatggcg ggctgcacga tccatttttg ctccctgata tggaaccggc
     1141 tgtcgagcgg ttacatcagg ccctcgctcg ccaggagcgg gtctgttttt acggcgatta
     1201 cgatgtcgat ggggtctcgg cgacgagtct ctatctgtcg ttctatcaag gtctcggcgg
     1261 gaatgcctgt ggctacattc cgcatagggt tcgcgaaggc tacgggttga acgagggggc
     1321 ggttcgtcgc ttagcggaag aggggatttt gctgcttgtc acctccgact gcgggacgac
     1381 ctcgcatcat gagattgcgg ttgccaggga actcggcatg gatgtgatcg tcacggatca
     1441 tcatcaaacg gatgccacga tgcctgccgc ccttgccgtg ctaaatccgc accggcgtga
     1501 tgcgatctat ccgtttaaag ggctctgctc ggcggggttg gcgtataagg tggtgtcggc
     1561 ttaccagcaa cggtatggat ccggcgacgt ggagcctgac tcctgcttgg atctggtcgc
     1621 gttggcaacg gtggccgaca tcgtgccgct tcaggacgag aatcggattc tggtgcggga
     1681 aggcctgacc cacatcacgc gcggagcccg cgctggaatt cgcgcgctca agcaggtagc
     1741 cggtatcgcg aaggcctgta cgagcgagac gatcggtttc cgcctcggcc cccggttgaa
     1801 tgccgcagga cggctggatc atgccatgct ctgtgtacgg ctgttgacga cggagtccga
     1861 tcaggaggcc atgcagattg ccgaacagtt ggagcagttg aaccggcagc ggcaacagat
     1921 cgaggaaggc attaccggcg aggcggcgac ctatctgcag caggatgagc cggccgcggc
     1981 gatcgtctta ggctcgcgtg actggcatct gggtgttgtg gggattgttg ccgctcgctt
     2041 ggtggaccgg ttccatcgtc cgagcatcgt gatggcgatc gatgggcagg gcatagggaa
     2101 gggctcggcc aggacggtgg aggggttcga tctgtatcag ggattgtccg agtgtaagga
     2161 tctgctggag gcctatggcg ggcacccgag tgccgctgga ctgacgctca aggaatcgcg
     2221 gttggaggaa ttccgccggc ggttctgtga ggtggtggct cagtgggccg ggagtgctcg
     2281 cacggtgccg accctgcacg tcgatgccga ggtgaagctg acggatgtca atttcgacct
     2341 gatccaggaa ctggaatccc tgcatccctt cggggccggc aaccccgaac ccaccttggc
     2401 tgttcgcggc ctggatgtgg tggacgcgcg agtcgtcggc gagaagcatc tgaagttgcg
     2461 tgttcggcaa gggcggtcat ttatcttcga tagcatcggc tttcgcatgg gctcgtttga
     2521 agagctgggg ctcagggccg gtcgaccggt ggacttggcc ttcagccccg agcgcaatca
     2581 ttggaacggc tacgatcggg tgcagttgcg tatcaaggcc cttcgcatga gcggtgaggt
     2641 gtcgtaacta tgccgcacga gacggtgaca gagctgagtc agctgatcga tcgggtgaag
     2701 agctacaacg tcgaggcgga cctcgatctt gtgcgccggg cttatgattt ttcggccaag
     2761 gcgcacgaag gacagatgcg ccgatccggt gagccctatt ttcggcatcc tctggcggtc
     2821 gcgggcctgc tgacccattt aaagacggat gtcacggcca tcgtcgcagg cctgctgcac
     2881 gacacgctgg aagatacgct ggctacgccg cttgagttgg aacagcgatt cgggaaggac
     2941 gtggtgcatc tggtcgacgg cgtgaccaag atcggcaaga ttacctttcg gaactacgag
     3001 gaaaagcagg cggaaaattt ccgcaagatg gtcttgtcga tggcggacga tatccgcgtc
     3061 gtcctcatca agctggcgga tcggctccac aacatgcgga ccctggaata tctgagcgag
     3121 gggaagcggc aacaaatcgc ccaggagacg ctggagattt atgctccgtt ggccaatcgg
     3181 ctggggatcg gttggattaa gaacgagctg gaagacctct gtctgaaaca tctcaagccg
     3241 gatgtctacg agtcgttgcg cgttcgggtg gcgaagcggg acgaagaccg gcagcagtac
     3301 atcattgaag tcatcgacct ggtgaagaag gccatggccg aggccggttt gcagggtgaa
     3361 gtctccggcc gccccaaaca cctgtacggc atctaccaga agatggagaa gcaatcgatc
     3421 tcattcgaag aggtgtacga tctcgcggcg ctgcgcatca ttaccgacat caagatgaac
     3481 tgttatgcgc tcctcggtgt catccattca ctgtggcgcc cgttgccggg ccgcttcaag
     3541 gactacatcg cgattccgaa atccaacatg tatcagtcgt tgcacaccac cgtcgtcggt
     3601 ccccagggtg aacatgtcga gtttcagatt cggacggagg agatgcatcg cgtttctgaa
     3661 cagggcatcg ccgcacactg gaaatacaaa gagcacgggc ggatcgacga gaaagacgga
     3721 aaggttttca gttggctacg ccagtttgtg gaatggaatc aggatttgcc ggacaatcgt
     3781 cagttcatgg attcggtgaa gctggatctt tttcacgatg tcgtctacgt ctttaccccc
     3841 cagggcatgg tgaaggaatt gcccaaaggc tccactccgg tggattttgc ctattccgtc
     3901 cacaccgaaa tcggcgacca ctgtgtgggg gcgaaggtca acggcaagat cgttccgctg
     3961 aaacacatga tggaaagcgg agacatggtg gagatcctga ccgccgctaa tcaaacgcct
     4021 caccgggatt ggttgaaatt cgtccgcacg tcgcgcgcga aaaccaagat caaacattgg
     4081 atcaaggccg aagagcaatc ccgcagcatc gagatcggca agcggttgct ggaagccgag
     4141 ttccgccggc atggcctggc gcccgcccag atgatgcggt cggaacagct cctggccgtg
     4201 gccaaacagg tcgggtatga aacgccggag gaactggccg cagccgtcgg cttcggccat
     4261 gtgccgacct cgcaggtgat gagcaaaatc aatccgcccg tcgcgacgga aggacagacg
     4321 gtcgcccctg aggttccgcc gccgccgaaa ccggtcaccg gacggaccga tgacacgggt
     4381 gtgcaggtca aaggcgcgcg ggacctgttg atgcaactct cccgctgttg caatcctgtg
     4441 ccgggcgacc gcatcttggg gtatatcacc aggggacgtg gactcacgat tcataccgtg
     4501 gattgtccca atttggaagc gttggactat gacaagaacc gactggtcga agtcgagtgg
     4561 gaccattcca cgcccagtac acactcggtc aaggtctcgg tcatcgccgt agacaagacc
     4621 ggagtgctgg cgcatgtctc gacggccatc tccgaatgtc aggccaatat cagtcgggct
     4681 gaaattacga cccgcgagga tcggaaggcg atgctggatt tcatcatcga ggtcctggac
     4741 accgcacatc tggctcgggt gctcaaggcg attgaaaggg tcgatggcat catcaccgtg
     4801 cgacgtgttc gctcctggca agagcgttcc tagcccgaga ggtgttcgtg tgttctcctc
     4861 cccgcatcag gtgagatccg catgagcctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnna cgattcttga tgccctgatc atcggcgaat ggctgatcga acgagggcag
     5041 cgctatatcc ccgatattgt ccggcagctg ctcatcggcg cggaagtggt ggctgccgga
     5101 gtgttgatcc tctggctggt catggggatc aacctcgtgg ccctggtggc gttgccgacc
     5161 gttgcggcgg cgatggtcgg tgtcgcgttg aaagatacgc tgacgcgctt cttttccgga
     5221 attgagctgg gaaagattgt gaaggtgggg gagtggatta cggtggttga tcgtgaaggg
     5281 atggtgaccc acatcggcat ggagcatgtg acgctgctga ctcgcgctca ggatctcgtc
     5341 tcgctgccta acgatctggt gatccagagc ggggtgacga atttcagcag gcccaccacg
     5401 acgcatttct gtaacgtgta tgttgatgcc gcctatcgca ctccgcctga gcaggtctgt
     5461 gcgacgttgc ttgaggcggc tgtggcggtg ccgggggtat tgcaggatcc gaaggcgacc
     5521 gctctggtca cggcatttaa cgaatcggcg attcagtatc gaatcaaatt cccgatcggt
     5581 gatttcgcac agcgggatat gatcgagagc cggatgcgga cgtacatttg gcatgccttt
     5641 gcgcgcaatg gaatcgagat tccctttccg cagcgtgtgg tgcatcagac ggtcgaaccg
     5701 gaggttgtga cgaacgggca ggcggtcggg cgcatggttg cccaactcgc ggcggtcgac
     5761 tttttgggta cactggatgc caagcagctc gaagcgttgg cgaaagaagc gcgatgggag
     5821 tcctacttac ctggcgaacg ggttgttcgt cagggggagc ccggagaggt cctctacgtc
     5881 atcgcttccg gaaaagctga tgtgcggttg gaacaggggg ggctcagttc gacggtgaca
     5941 acgcttgaag cggggaagtt tttcggcgaa atgtctctgt taactgggga gccgcgttcg
     6001 gccacggtat cggccgcgac ggaattgttg gtcattgccg tcgggaagca gtcgttgctg
     6061 caggttgttc aggaggatcg ccgactgatc gagcgcatcg gtgaagtggt ggcccgtaga
     6121 caggcggcga ctgcggcagc gaaagcgcaa ctgtctcgtg aggcagcggc cctttcggca
     6181 gttacgcaca cacgttccct cgtcgagcgc attcagaact ttttctgggg tgcgcgcaag
     6241 gcgcagtgat caggttgaag gctggtcagg gctgtttgaa gctcagtttc ataccgcgtt
     6301 gaagctgcag tgccgctgca cggcccccgc caagttccag gacgtacagc gcaccttcat
     6361 ccgagtggta ctgtggacag ccgtcgtcct gtctggtgca gatggggacg tttcgttcga
     6421 tatggacgat cgttttcttc gcgtccatcc agatgatatc gagtgggatc ttggtatttt
     6481 tcatccagaa tgtccagggc tgggcgtcgc cgaagataaa gagcatgccg cggtcatccg
     6541 cgagatgctc tcgaaacatc aaaccctggg cacgtttcaa tgccgtatcg gctaattccg
     6601 cctggatcag tgtaccggtt ggcgttttga ccgtaatgag cgtatcggcc tgcggatcgg
     6661 cggccaagct gaagcaggtc atactcagca tggtgatacc tgtaaggcac cacatcgcaa
     6721 gtctcagtag tgccggacag tgtgtattat caaggcgatc tgttgtgtcg agcgtcacgg
     6781 ctgcatccta gcgggctcct cgcgggccgt caagatactg aacgtgtccg ggggctgagt
     6841 gtgcgacgct gtgttgcaat ttcgtcggcg cctatgccat cctcggccta ttatttgact
     6901 tggatggagg ctgttcatga aagaaacgcg acgtgtggtg tacatgcgag gcgtgtt
//
LOCUS       DAOQRM010000131         6904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10800_length_6904_cov_13.754417, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000131 DAOQRM010000000
VERSION     DAOQRM010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10800_length_6904_cov_13.754417"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1742)
                     /locus_tag="PKK81_10125"
     CDS             complement(222..1742)
                     /locus_tag="PKK81_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33280.1"
                     /translation="MEVAMNAPLLERYGDRIAGVLTCYDRMVITGTLPGPCYAAGMTS
                     FLNARQIRIFDYPRFAEPLRDTIRANAEQLAEAASVKIEYLAKAHIRKEDVIAGVIKT
                     RGDHPGLVHVISVMEACSTYKAWHDKRTHQTSLRPTTGKCLHYYFYFIDAELGLVYLR
                     VPTWCPFRLQFYCNGHRWLARQLTAAGVGYTLADNAFIRIDDWDRAQQLADRLSPDVL
                     HRILDRYAQACCPVLDVFAQRYHWSLMQIECSTDLAFRSDAIMKPLYAALSREAILSV
                     KAEQVMHFLGKKMTPQLAQEVDSRLSTRIEGICIKHRMGKNSVKMYDKFGRVLRIETT
                     TNDVSFFKHHRQVEHRDGQATRELATVKKSIYSLIDLREILLGCNRRYLEFLSALDDF
                     SAGSRRLDKLTAAKTVDGHRVKGFNFFDKTEHTLLCSLQRPQFNISGVRRADLKPFLP
                     NLSVASITRYLKRLRVLGLIKKIAHAYRYYLTRLGRNAIAAACHLTEQIIVPALAA"
     gene            2334..2924
                     /locus_tag="PKK81_10130"
     CDS             2334..2924
                     /locus_tag="PKK81_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33281.1"
                     /translation="MLKHIWGHQIPRVLREPALFDKTGLPKYWATVWSMFHTADLAPS
                     SKRKVLSHVEALYQFSETLHGPGMLDDALFSVDIEKLGQLLKAYFVSIRNLPNINEAA
                     QLKWQTAFRFVSDIIRRLSKSDLPIAQLQQFQGRLIGLTLARLSAPVTYSSNKFRLFG
                     VGPYERKNRIHEPAGGVANSWSGMIGSSMAESDSFG"
     gene            3294..3575
                     /locus_tag="PKK81_10135"
     CDS             3294..3575
                     /locus_tag="PKK81_10135"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNV33282.1"
                     /translation="MSAAVSTARLEARISADLHSMLKRAAELQGRTMTDFVVTAVQAA
                     ARSAIEQAEVVRMSLADQECFAQALLSPPSPSPALDRAFARRSKLLRSE"
     gene            3572..4093
                     /locus_tag="PKK81_10140"
     CDS             3572..4093
                     /locus_tag="PKK81_10140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV33283.1"
                     /translation="MSEAPFGIESLNAGHDRLSFRSGSDPLDRYFREQATQDVKRRVA
                     ACFVAVDDSRAIAGYYTLASAGILLADLPHELGKRLPRYPTVPAVRMGRLAVSQNFKG
                     MGLGGALLADALNRSACSEIAAYALVVDAKDGEAAGFYLHHGFVALPSTPLTLFLPLA
                     TALASRKVGKAAQ"
     gene            4645..5670
                     /locus_tag="PKK81_10145"
     CDS             4645..5670
                     /locus_tag="PKK81_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33284.1"
                     /translation="MSDVPGATVPASRLSTVLENIEQDKKLSANAFEYLRQQGYLALL
                     SLASGESNYQEFSNLARVEQGRRESAAAEVRQKEEAERLRAIAESAAKEAARTAEYER
                     QRRIQESDPKYVAKMKNQRLRARYGLDQFIEKESFNRLMGILHRIDGGARFSDEDVLW
                     LTTEGSDYYSDELRFEFHSREAAFFVTEFARTRDPWNAVNASGHFRKCDRSGAAHELL
                     EGVSLEGLKSPKLKSAVRTTHGGVMRDLGRFDVALRYGTEAHAITDSDFRPCTLLGAV
                     NFELGNYESARDWYAKAAERGASERSIDDELRGIYMRADKSKRDEIRAFLIREDPVRY
                     KWAHSLR"
     gene            5827..6408
                     /locus_tag="PKK81_10150"
     CDS             5827..6408
                     /locus_tag="PKK81_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33285.1"
                     /translation="MSSELEEDFEIEDPAEELFRACEKVLRSGIWLIRNGYGRLAILP
                     YLSPSGCHWRCEFHPVGFRSRTMFRYTSADGHKYLAAHCGGTLRKDASPEKLAKAALV
                     GADEDLLSACSGEASPETLAWLEVLERTLGQGFVPQAFHEYSTDPSSWDVVTPASDFK
                     SSISAPPWNWEQDLSWKDLPYWGSSQKTEFKVR"
     gene            complement(6405..>6904)
                     /locus_tag="PKK81_10155"
     CDS             complement(6405..>6904)
                     /locus_tag="PKK81_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003117273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HNV33286.1"
                     /translation="KQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQ
                     SVELRRRVQAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLW
                     NTVYLERAANALRNHGQGVDDALLQYLSPLGWEHINLTGDYLWRSSAKVGAGKFRPLQ
                     PLPKP"
BASE COUNT         1457 a         1995 c         2025 g         1427 t
ORIGIN      
        1 tttgtcactt tgatgtcgcg tgtcctgggg gccaatctgc tggcgccagt cgaaggacat
       61 gacgaggtgc atttccacta aacttcccgc agggcgtgtg gcacttgcca aacgccctgc
      121 tcccttcggg aattccccac atggcatcga gtacaaattt cgttactgta cgtatttagt
      181 cccttatgac tcaactcttc acaaactctg agcagatttt gtcatgcggc gagtgctggg
      241 acgatgattt gttcggtgag atggcaggcg gcggcgatgg cgttgcggcc aagtcgggtc
      301 aggtaatagc ggtaagcgtg agcgattttc ttgatgaggc ccaagacacg gagacgtttc
      361 aggtagcggg tgatactggc cacggagagg ttgggcaaga agggtttgag atcggcacgg
      421 cgcacgccac tgatgttgaa ttgcgggcgt tgcagggagc agagcaaggt gtgctcagtc
      481 ttgtcgaaga agttgaagcc cttgacgcga tgaccgtcga ccgttttggc agcggtgagt
      541 ttgtcgaggc ggcgactgcc ggcggagaag tcatcgagcg cggagaggaa ttcgagatag
      601 cggcggttac agccgaggag aatctcgcgc agatcgatca gactgtagat cgacttcttg
      661 acggtcgcca gttcccgggt ggcctggcca tcccggtgtt ccacctggcg gtggtgcttg
      721 aagaaggaaa catcgttggt ggtggtttcg atgcgcagga cgcgaccaaa cttgtcgtac
      781 atcttgaccg agttcttgcc catccgatgc ttgatacaga tcccttcgat gcgcgtggac
      841 aggcggctat ccacttcctg cgccagttgc ggcgtcattt ttttgcccag aaagtgcatc
      901 acctgttcgg ccttgaccga aagaatggcc tcccgcgaaa gggctgcgta gagcggcttc
      961 atgatcgcgt ccgaacggaa ggcgagatcc gtcgaacact cgatctgcat caggctccag
     1021 tggtaacgct gagcgaagac gtccaggaca ggacagcagg cctgggcata acggtcaaga
     1081 atccggtgca atacgtccgg ggacaggcga tccgccaact gttgggcccg atcccagtca
     1141 tcgatgcgga tgaaggcatt gtcggccagg gtgtaaccca cgccggcggc ggtcagttgc
     1201 cgggccagcc agcgatggcc gttgcagtaa aactggaggc ggaacggaca ccaggtcggc
     1261 acgcgcaggt agacgagccc caactcggca tcgatgaagt aaaagtagta atgcaaacac
     1321 ttgcccgtcg tcgggcgcaa ggaggtctgg tgagtgcgct tgtcgtgcca cgccttgtag
     1381 gtcgagcagg cttccatgac cgaaatgaca tgcaccagcc cgggatgatc gccacgcgtc
     1441 ttgatcaccc cagcaatgac gtcttccttg cgaatgtgcg ccttggcaag gtactcaatc
     1501 ttcacgcttg ccgcttccgc caactgctcg gcattcgccc ggatagtgtc acgcaacggt
     1561 tcggcaaagc gcggatagtc aaagatacga atctgacggg cattcaggaa gctcgtcatc
     1621 cccgccgcat aacacgggcc gggcagtgtg cccgtgatca ccatccggtc gtaacaggtc
     1681 aacacgccgg cgatccggtc accatagcgt tcaagaagcg gtgcgttcat ggcaacctcc
     1741 acttcgcgtc acagcgcgct cttactttaa cctgtttggt tccggctcgt ccggcttagg
     1801 agaagtcatg ttctatgatg aaaaacagcg cggggtcgct tggttgctcc cgccaaggat
     1861 ttctgcggcg gcttggttgc atggtcaacg agactgagtg tcagccaacg cagcgattcc
     1921 ctaaaaaagg tgtgcctagt ttgtaggcag gtcgcctaaa tccttggcca ggcaggtctt
     1981 atagaagact gccaccagcc cactcacagg tttatccaca aaatctgggg atagaaggtt
     2041 ttgccagtct gtccatatgg taatggacaa tacttttggc attgatgcgc aacggctacc
     2101 gagcgccgga tcgtactcac acaaccggcc agaagcggtc gatcaacgta gcgagagggc
     2161 agctgccgac ctgtgggcaa gtggtgggaa acgcatggtt catcgcgttt tccacggctt
     2221 gtccataggg aaccgcgctt gcgcggttag gcgggtatcg gcgtagccga ctgtccacaa
     2281 atccacaggg tctcgttttt gagcattggt tttttcttgg gaatggtgac gagttgctaa
     2341 aacacatttg gggccaccaa attcctcgtg ttctacgtga gccagctctt ttcgacaaga
     2401 ccggattacc aaaatattgg gcaacggtct ggtcaatgtt tcataccgca gacttggcgc
     2461 catccagcaa gcgtaaagta cttagtcatg ttgaggctct ctaccagttc tctgaaactt
     2521 tgcatgggcc cggcatgctt gacgatgcat tgttcagtgt cgatatcgag aagctgggcc
     2581 aactcctcaa agcttatttc gtttcgattc ggaatcttcc gaacatcaac gaagcagctc
     2641 aattgaagtg gcaaacagcc tttcgattcg tcagcgacat catccgccga ttgagcaaga
     2701 gcgatttgcc tatcgctcag cttcaacagt ttcaagggcg gctaatcggc ttgaccttgg
     2761 cccgactcag cgcgccggtc acatattcgt cgaacaagtt caggttgttc ggtgtcggac
     2821 catacgagcg caaaaatcga atccacgagc ccgcaggggg agtggcgaac tcttggtccg
     2881 gaatgattgg atcttccatg gctgaatccg attcgtttgg ttgaacgaag ccgcacggcc
     2941 tcgcctgaca tccaagagcc agagcgactc ttcgttgcca gaatgcgaag cgcattatag
     3001 tccagcttca tcgactggat gatccagttc gcccccgcta cagattagaa atctggcggc
     3061 aatctcgccc catgaccaat caatttcatc agccaacact cgccacatcc ccggacatcg
     3121 ttggctggcc gagaacgcgg cagcggcttg atcaacgatg acatcaattt tttccagcct
     3181 acctcagttg ctgacagtag cggaaaagct caactccaac gtgcacagca acaccatgat
     3241 agattggctt tgtacggcag ttggccgtat aattcgacta ggaggattca cccatgtcag
     3301 cagctgtttc aactgcccgc ctcgaagccc gcatcagcgc agatctgcat tcgatgctca
     3361 aacgtgctgc cgagcttcag gggcgtacca tgactgattt cgtcgtcacc gccgtccagg
     3421 ccgccgcccg ctccgccatt gagcaggccg aggtggtgcg gatgtctctg gcggatcagg
     3481 agtgctttgc ccaggcattg ctgtccccgc catccccttc acccgctttg gatcgcgcct
     3541 tcgcccgtcg tagcaagctg ttgcgttcgg aatgagcgag gctccgttcg gcatcgagtc
     3601 gctcaacgcc gggcatgatc gactgtcctt ccgcagtggg tcggatccgc tggatcggta
     3661 tttccgggaa caggcgacgc aggatgtgaa gcgccgcgtg gcggcgtgct tcgtggcggt
     3721 ggatgactca cgcgcgatag ctggctatta cacgctggcc tcagccggca tccttctggc
     3781 tgacctccct cacgaactcg ggaagcgtct gccacgctat ccaacggtgc ccgcggtgcg
     3841 catggggcgg ctggcagtct cccagaattt caagggcatg gggctcggcg gcgcgttgct
     3901 ggccgatgcc ttgaatcgct cggcctgctc cgagattgcc gcctatgcct tggtggttga
     3961 tgccaaggac ggggaggccg cgggcttcta tctccaccac ggatttgtcg ccctgcccag
     4021 tacgccttta actcttttcc ttccattggc aacggcgctg gcatcccgga aggtgggcaa
     4081 ggccgcccaa taggcaagtc tcttgcatga caaaggccac cgaagaggcc ttgttggtga
     4141 tgcagatggc gccgcccacg cggttcttgt aggtgccgcc cgctgattga cactagaagt
     4201 gtgaagcgca accccctttc tgcaggtgtt gcgcaacttg aaaaaattag gggcttaact
     4261 gttgcgccaa tcgaatatag agtgcgcaac aatagacgga taccttaaac gcaacggcag
     4321 ttttcaacaa aatgtcaatg gatcctgtta taccgacact atccagtgtg catcattacc
     4381 cgaaattacc tagattgcga agggcgaacc gcgtcggtac cggcgcgctc ccgatgccgc
     4441 ggccggcaac ctttggtatt agaatccgca tggaatactc ggcagcctgc cgacgagcgc
     4501 cgacaagagc ttaccatgag cagaacccca cgaatcctta ggcagccaaa tccgaatttc
     4561 agacgctccg ggcgtcccga agcgggtcct caacagcggg tagtcataga tccgggacga
     4621 tccgcgatcg ccggcaagta tttcttgtcg gacgtcccgg gagcgaccgt tcccgccagc
     4681 cggctcagca ccgtcctcga aaatatcgaa caggacaaga agctttcggc gaatgcgttc
     4741 gaatacctac ggcaacaggg gtatctcgcc ttattgagcc tggcttcggg cgagtcgaac
     4801 tatcaagaat tctctaatct ggcccgcgtc gaacagggaa ggcgggaatc cgccgccgct
     4861 gaggtcaggc agaaggaaga agccgaacgt ctgcgggcca ttgcggaatc tgcggccaag
     4921 gaagccgccc ggacggcgga gtacgagcgc cagcgacgga tccaggaaag cgatccgaaa
     4981 tacgtcgcca agatgaagaa ccaacggctc cgggcccgat acggcctgga ccaattcatc
     5041 gagaaggaat cgttcaaccg cctcatgggc atcctccacc ggatcgacgg aggcgctcga
     5101 ttcagtgacg aggacgtgct gtggctaaca acggagggct cggattacta ttccgacgaa
     5161 ctcaggtttg agttccactc cagggaagcc gcgttcttcg tgactgaatt cgcccgaacc
     5221 cgcgaccctt ggaacgccgt caacgccagc ggccatttcc gaaaatgcga ccgctcggga
     5281 gccgctcacg agctattgga gggcgtttcc ttggaaggcc tgaaatcacc gaagctgaag
     5341 tccgccgtcc gtacgaccca cggcggagtc atgcgcgatt tgggccggtt cgacgtggcg
     5401 ctccgctacg gaaccgaggc gcatgccata acggacagtg atttccgacc ctgcacgctg
     5461 ctcggtgctg tgaatttcga gctgggcaac tacgaatcgg cacgggattg gtacgccaag
     5521 gcggcggaac ggggtgcgag cgaacgctcg atcgacgacg aactgcgcgg catctatatg
     5581 cgggccgaca aatcgaaaag ggacgaaatc agggcgttcc tcatcaggga agatccggtc
     5641 cgatacaaat gggcccatag tctccggtag ccgcagtcca agacgccgag tcgtcgcgtc
     5701 tgcgaaaacc gcttttcccg cccactgggg ccggagtggc ggtttttgta ccaacggcac
     5761 aatccataca tcatggttta ccatggcaca ttccgccgcc aagtgcgaat agacgaggaa
     5821 cggacgttgt cgagcgaatt ggaagaagat ttcgagatcg aggatcccgc agaggaactc
     5881 ttcagggcgt gcgagaaagt cctacgctcc ggcatctggc tcatccggaa cgggtacggc
     5941 cgcctcgcga tcctgccgta cctctccccg tccggctgcc attggcggtg cgaattccat
     6001 ccggtcggat tccgatcgag gaccatgttc cgctacacga gcgccgacgg acacaaatac
     6061 ctggccgccc attgcggcgg aacgctcagg aaggacgcct ctcccgaaaa gcttgccaag
     6121 gccgccctcg tgggcgccga cgaagacctg ctttcggcgt gctccggaga agcctcaccg
     6181 gaaaccctcg cctggctcga agtcttggag cggactctcg ggcaggggtt cgttccgcag
     6241 gccttccacg aatactccac cgatccatcg tcatgggatg tcgtgacgcc ggcctcggat
     6301 ttcaaatcct cgatctccgc gcctccgtgg aattgggagc aagacctttc ctggaaggac
     6361 ctcccctact gggggtcgtc tcagaaaacg gaatttaaag tacgttaagg ctttggcagc
     6421 ggctgtagcg gcctgaactt ccccgcaccg accttggcac tgctgcgcca gaggtaatcg
     6481 ccggtcaggt tgatgtgctc ccagcccagc ggcgacaggt attgcaacag tgcgtcatcg
     6541 acgccctggc cgtgattgcg caatgcgttc gccgcccttt ccagatagac ggtgttccac
     6601 aacacgatgg ctgctgtcac caggttgagg ccgctggccc ggtagcgttg ctgttcgaag
     6661 ctgcggtccc ggatttcgcc cagccggttg aagaacaccg ccctggccag cgcgttgcgt
     6721 gcctcgccct tgttgagtcc ggcctgcaca cggcggcgca gctccacact ttgcagccag
     6781 tccagaatga acaacgtgcg ctcgatgcgc cccagttcgc gcagggcgac ggccaagccg
     6841 ttttggcgcg gatagctgcc gagcttgcgc agcatcagcg aggccgtcac cgtgccctgc
     6901 ttga
//
LOCUS       DAOQRM010000132         6843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10950_length_6843_cov_18.559664, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000132 DAOQRM010000000
VERSION     DAOQRM010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10950_length_6843_cov_18.559664"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..494
                     /locus_tag="PKK81_10160"
     CDS             126..494
                     /locus_tag="PKK81_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33287.1"
                     /translation="MKFVIGSVATLAGVLFMCSMASANPALLPKHEGYPMKNDGSPVN
                     GQPTANDPGQKAGGGESTLQKSADSSSKHAEQKLVKSDNARITEGQGAGRLPNVQGPQ
                     IKIEPPVTSATKITGDRKID"
     gene            complement(571..1140)
                     /locus_tag="PKK81_10165"
     CDS             complement(571..1140)
                     /locus_tag="PKK81_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNV33288.1"
                     /translation="MSRLNQKILDRSYFSCSTVEVARSLIGKYLVRNNGAGLMAGKII
                     EVEAYVGPEDKACHASKGRTARTEVLFGPPGIAYVYLCYGMHEMLNVVTEQDGYPAAV
                     LLRAVECDGSLIDGPGRLTRAFGIDRRLNRWDLTVGDTLWFEDRGDSLPQDALKTYPR
                     IGVDYAGEWATKPWRFRLQREAGSSRTKR"
     gene            complement(1316..3361)
                     /locus_tag="PKK81_10170"
     CDS             complement(1316..3361)
                     /locus_tag="PKK81_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNV33289.1"
                     /translation="MRWIGWLIGAGAMVLSCGLVAFAHDPDAPDLDVPEVTVEADRPV
                     AASSQQFIPDKEYLLQPQGRPAQVLRLIPGFIAVEHSGGAGKADQYFLRGFDADHGTD
                     VAFFADGMPINLRSHAHGQGYTDLNFIIPETIEGLDVYKGAYLPEYGDFSTAGAVNFR
                     TREVVKEGVVQSAGGQFHTQRHLLMVSPTTDRVRSLIAAEGYYTNGPFEHDNRYFRGN
                     LLGKATMNPSARSELSVTGTFHKSQWNASGEIPLRAVQDGSLDRFGAVDPSEGGRTMR
                     STGRLNYHYDTTSGGRFFANAYAQYYRFDLFTNFTFFQNDPVNGDGFQQSDRRIMYGG
                     DIGYKQNGRWFDMDGAATVGVQSRVDHIHARLGPQVLRNPLGSTSDTDITEASYAPFF
                     KLEVQPAPWMRLAGGIRSEVFTFDVRNRCQTCVTQPSGTADSGLMLPKANLILGPWLR
                     TEFFLNYGEGYHSNDARSAVTPAASPLARAKSYEVGLRSRPWGSDGIELTATVWALDM
                     KQELVFVGDEGTTEIRGASRRRGMEVGARGQVWGPVYFNGSITWTKAEFRNGDAIPLA
                     PEVTAYGALLLRWPEGLTSQLQATYLGVRPLTEDCSIRAPSWIDIDLSERYQIPIKLA
                     HGRLEAFLFVQNLLDTKWEQAVFAFESRLRNEATGVTDIHFVPGNPRMVMGGVAWYF"
     gene            complement(3488..3907)
                     /locus_tag="PKK81_10175"
     CDS             complement(3488..3907)
                     /locus_tag="PKK81_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HNV33290.1"
                     /translation="MTICVVDGRGGGLGSRMVAGLRGLVDDGHAIIGLGLNSASAEAM
                     ARAGATFTETAPQMIHRRLYAADVIVGSLSLLMPGSMLGEVTPVLAQAVLESSARKVL
                     LPLNKRKVEVVGVEGRTLDALIDHALQRCVCLLQATA"
     gene            complement(3907..4326)
                     /gene="nikR"
                     /locus_tag="PKK81_10180"
     CDS             complement(3907..4326)
                     /gene="nikR"
                     /locus_tag="PKK81_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNV33291.1"
                     /translation="MKQLARFGVSLDEDLLADFDRLIERRHYTNRSEAIRDLIRDNLV
                     GQQWDENRETVATITFVYDHHVPGLTGKLTHIQHDFQGHIMAGMHVHLDHDHCLEVLV
                     AKGKGAAIRKVADALLSVKGVKHGKLTMTTTGKGLSI"
     gene            complement(4462..5535)
                     /locus_tag="PKK81_10185"
     CDS             complement(4462..5535)
                     /locus_tag="PKK81_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV33292.1"
                     /translation="MSDLDASAVKLLLIDDELPSLKLMEAILKQAGYEKITTTTDPRQ
                     AVPLYQEVKPDLIATDMRMPHMDGFEVLRQLTAVIPAEDYVPILVITGELDAPTKHRA
                     LAEGAKDFLNKPVDATEVVLRIKNQLVTRQLHRQLRMHNEHLEAQVRLRTKVVEQTQL
                     DLLNRLALAAEYRHDATGGHAWRVGRMAMLLAEMKGLPADQVDMIKKTAPLHDIGKVG
                     LRDSVILKDGPYVTADWEAMKQHTKIGSRLLSDSRAPLLMMAREIALTHHERWDGAGY
                     HGIKDVQIPLAARIVALADAFDIMTHPCSYKATMTLSEAKGEIAAQAGKQFDPELSAL
                     FMQLIDREGGKLLAATGPLQMVA"
     gene            complement(5590..>6843)
                     /locus_tag="PKK81_10190"
     CDS             complement(5590..>6843)
                     /locus_tag="PKK81_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV33293.1"
                     /translation="ASDLAERKRAETSLRESHTQLEAAMTELKTTQQQMVQQERLRAL
                     GQMASGIAHDFNNTLSPIMGFSELLLIAPQMAADPAQLKEYLQTINMAAKDAAKVVSR
                     LRDFYRPRLDADLAGIVDLNSLVQQSVKLSQPKWKDQALANGVAIEIKTELEAVPAVG
                     GHESELREVLTNLVFNAVDAMPKSGSITLRTKLDGDAVRVEVSDTGTGMTEEVRQRCL
                     EPFFSTKGEKGSGLGLAMVYGIIRRLRGTIDITSAVGVGTTFSIRLPIQAEAETGTEH
                     DGHNMDGVRLQILVVDDDPLVGRVTGEYLRVAGHSVEVVGSAAEAIRRFNPERVHLVV
                     TDHGMPHMTGRQLADVIKKVSPDTPVLLLTGGDELEPDGTVSQAVDAELSKPLTMGAL
                     RQALSTLRYPKSLPAESQKEIGDAA"
BASE COUNT         1360 a         2226 c         1868 g         1389 t
ORIGIN      
        1 ccgctccctt ccccccttta tccttgacac ggcttcttca gatagctaag attcccgcac
       61 tcatcaggct tttaacgtct ataatcgacc accatacttt cacattcttt caaaggagta
      121 ggggtatgaa attcgtgatc ggcagcgtcg cgacccttgc aggtgtattg tttatgtgtt
      181 cgatggcctc cgcgaatccg gccctgttgc cgaagcacga aggttatccg atgaagaacg
      241 acggcagccc ggtgaacgga cagccgacag cgaatgaccc aggccagaag gctgggggcg
      301 gtgagtccac cctgcagaag tccgcagatt cctccagcaa gcatgccgaa cagaaattgg
      361 tgaagtcgga caacgcgcgg atcaccgaag gccagggcgc cggccggtta ccgaacgtcc
      421 agggcccgca gatcaagatt gagccaccgg tgacctctgc cacgaaaatc actggcgacc
      481 ggaagatcga ctagtttcta ttcgatagaa cgaaaacggg gccgttgtct cacgacaacg
      541 gccccgtttc tttttgtccc atgatcgcgc ctaccgcttc gttcgacttg agccggcctc
      601 cctctgcaat cgaaacctcc acggtttcgt cgcccattcc ccggcatagt ccacaccgat
      661 ccgcggatag gttttgaggg cgtcttgtgg caacgagtcc ccgcgatcct caaaccagag
      721 cgtgtccccg accgtgagat cccaccgatt caatcggcga tcgatgccga acgcacgggt
      781 cagccggccc ggcccatcga tgagcgaccc gtcgcactcc accgcccgca agaggacggc
      841 cgccggatag ccgtcctgct cggttacaac gttcaacatc tcgtgcatcc cgtagcagag
      901 atacacatag gcaattcctg gcggaccgaa cagcacctcc gtcctggccg ttcgtccttt
      961 cgacgcatgg caggccttgt cctccggacc gacataggcc tcgacctcga tgatctttcc
     1021 ggccatcaac cctgccccat tgttccgtac aagatacttg ccgatcaacg accgcgcgac
     1081 ttcgacggtt gaacagctaa agtaggaacg atccaggatc ttctggttca gtcgactcat
     1141 tggaaatcct ttatgttccc ccccatgtga gttgacgccg atcggatagc aggctgctca
     1201 aaaagcccgt tcagcaaggc cgcagccgaa ggtaccgccg gaggcgtagc ccattggcta
     1261 cgttgaggac ggtaccgagg cgagaacgac gctggcgggc tttttcagca gcctgctaaa
     1321 aataccacgc aacgcccccc atgaccatcc tcggattccc cggcacgaag tggatgtccg
     1381 tgacacccgt tgcctcattc ctgagcctcg actcaaaggc aaagaccgct tgttcccact
     1441 tcgtatcgag tagattctgc acgaatagaa acgcttcgag tcgcccatgc gccagcttga
     1501 tcggaatctg gtaccgttcg gacaagtcga tatcgatcca ggatggcgcg cggatgctgc
     1561 aatcctccgt cagcgggcga acaccgagat acgtcgcttg cagctgggag gttagtcctt
     1621 ccggccaccg cagcagcagc gcgccatacg ccgtgacctc cggcgccagg ggaatggcat
     1681 cgccgttgcg gaactccgcc ttggtccagg tgatgctgcc gttgaaatac accggccccc
     1741 agacctgccc gcgcgccccg acctccatac cgcgccgacg ggatgcgcca cgaatttctg
     1801 tcgtcccttc gtcaccgaca aacaccagtt cctgcttcat gtccagcgcc cacaccgtgg
     1861 ccgtcagctc gataccgtcc gaaccccatg gtcttgatcg aagtccgacc tcataactct
     1921 tggctctcgc cagcggcgag gcggccggag tcaccgccga gcgcgcatcg ttgctgtgat
     1981 atccctcgcc atagttcaga aagaattccg ttctcaacca gggccccagg atcaggttcg
     2041 ccttgggcaa catcagaccg gagtcggcgg tccctgacgg ctgcgtgacg caggtctgac
     2101 agcgattgcg cacatcgaag gtaaacacct cgctccgtat gccgccggcc aggcgcatcc
     2161 atggtgccgg ttgcacttcc agtttgaaga agggggcgta agaggcttcg gtgatatccg
     2221 tatcgctcgt gctcccaagc ggatttctga gcacttgcgg cccgagccgc gcatggatgt
     2281 gatcgaccct gctttgcacc ccaacggtgg cggcgccgtc catgtcgaac catcgtccgt
     2341 tctgtttgta tccgatatct ccgccataca tgatccgccg atcggactgc tgaaacccgt
     2401 cgccgttgac gggatcgttc tggaagaacg taaaattcgt aaaaagatcg aagcgatagt
     2461 actgcgcata ggcattggca aagaaccgtc cgcctgacgt cgtgtcgtaa tgatagttga
     2521 gccggccggt gctgcgcatc gttcgcccgc cctcggaagg atcgaccgcg ccgaaccgat
     2581 ccaacgaccc gtcctgcacg gcgcgcaagg ggatctcccc ggacgcattc cattgagact
     2641 tgtgaaaggt accggtcacc gacagctccg acctggcgct cggattcatc gtcgccttcc
     2701 cgagcagatt gccccggaag taacgattgt cgtgctcaaa cggtccgttc gtgtagtacc
     2761 cctcggcagc aatcagcgag cgaacacggt ccgtcgtcgg ggagaccatc agcaggtggc
     2821 gctgcgtatg gaactgcccg ccggccgact gcaccacgcc ctccttcacc acctcgcgcg
     2881 tcctgaaatt gaccgccccc gccgtggaga aatcgccata ctccggcaga taggccccct
     2941 tgtaaacatc gaggccctcg atcgtctccg gaatgatgaa attgaggtcg gtatacccct
     3001 ggccatgcgc atgggaacgc aggttgatcg gcatgccgtc ggcaaaaaat gcgacatccg
     3061 tgccgtgatc cgcatcgaac ccgcgcagaa aatattggtc ggccttgccg gcgccgccgg
     3121 aatgttcgac ggcaataaat cccgggatga ggcggagcac ctgggccggg cgcccttgcg
     3181 gctggaggag atattccttg tcggggatga attgttgcga cgaggccgcc accggacggt
     3241 cggcctcgac agtcacctca ggcacgtcga gatcaggcgc gtcgggatcg tgggcgaacg
     3301 ccaccaagcc gcagctcaac accattgctc ccgcaccgat caaccatcca atccaccgca
     3361 taatcgctcc caatcacatc acatgagcga actaggcgct cagaatggtc cacgcgtcgc
     3421 ccctagcggc cgcttcagac cctcgtgatc tgactgtgca atctgagatg taaatcggcg
     3481 gtttcgatca ggcggtcgcc tgaagcagac agacacaacg ctgcagggca tggtcgatta
     3541 acgcatcgag ggttcgtccc tcgacaccga cgacttcgac cttccgcttg ttgaggggga
     3601 gcagcacctt tctggcggac gattccagca cggcttgcgc gagtacggga gtgacttcac
     3661 ctaacatcga tcccggcatg aggagactca acgagcccac aatcacatcc gcagcataca
     3721 gccggcgatg gatcatctgc ggggccgttt cggtgaaggt cgcgccggct cgagccatcg
     3781 cctccgcgga ggcgctatta agccccaggc cgataatggc atgaccgtca tccacaagcc
     3841 cgcgcaaacc ggcgaccatc cggcttccca ggccgccgcc acgtccatcc acaacacaaa
     3901 tcgtcatcaa atgctgagtc ccttacccgt cgtcgtcatg gtgagcttgc cgtgcttcac
     3961 gcccttgacg ctcagcaacg cgtcggccac cttgcgaatg gcagcccctt tgcccttggc
     4021 caccagcacc tccaggcagt gatcgtggtc gagatgcaca tgcattccgg ccatgatgtg
     4081 tccctgaaaa tcgtgctgga tatgggtgag tttcccggtc aaacccggca cgtgatggtc
     4141 gtacacgaac gtgatggtgg caacggtttc cctattctcg tcccattgct gcccgaccag
     4201 attgtccctg atcaagtcgc ggatcgcttc ggagcggttc gtgtagtggc gccgttcgat
     4261 cagacggtcg aaatcggcaa gaagatcctc atcgagcgac acgccgaatc gcgcaagctg
     4321 tttcatggcc gtgatttcag tgttacgaaa ttaattatcg tggcacggat aatacaaagc
     4381 ccggcgcaga tgcgtcaagc agccgtgaag agaatgtttg gtgaggcgcc agcgggagga
     4441 atcggaccga aaagagagct gttacgcaac catctgaagc gggcccgttg cggccaacag
     4501 tttcccccct tcacggtcga tgagctgcat gaataaggcg ctcaactcgg gatcgaactg
     4561 tttgccggcc tgtgcggcga tttcaccttt ggcttcgctg agcgtcatcg tcgctttgta
     4621 tgagcaggga tgcgtcatga tatcgaacgc atcggccagc gccacgatcc gcgccgccag
     4681 cgggatctgc acatctttaa tcccgtgata gccggctcca tcccaccgct catgatgcgt
     4741 cagggcaatc tcgcgggcca tcatcaacag aggagcccga ctatccgaca ggagccgcga
     4801 gccgattttg gtgtgctgct tcatcgcctc ccaatcggcc gtcacatagg gcccgtcctt
     4861 caggatcacg gaatcgcgca ggcctacttt cccgatatcg tgcaacggag cggtcttctt
     4921 gatcatatcc acctgatccg caggcagccc cttcatctcc gccagcaaca tggccatccg
     4981 tccgacccgc cacgcatggc cgccggtcgc gtcatgccga tattccgccg ccagcgcgag
     5041 acggttcagc agatccagct gcgtttgctc cacgactttg gtgcgcaagc ggacctgcgc
     5101 ttccaaatgt tcattgtgca tacgcagctg tcgatgcaac tgcctcgtga ccaattggtt
     5161 tttgatgcgg agcacgactt ccgtggcatc caccggcttg ttcaggaaat ccttggcgcc
     5221 ctccgccaaa gccctgtgct ttgtcggcgc gtcgagctcg ccggtaatga cgagaatcgg
     5281 cacatagtct tcggcgggaa tgacggcggt caactgccgc agaacttcga atccatccat
     5341 atgcggcata cgcatgtccg tcgcgatcag atccggcttc acctcttgat ataacggcac
     5401 cgcctgacgc gggtcggtcg tcgtggtaat tttttcgtag ccggcctgct tgaggattgc
     5461 ctccatcagt ttgaggctag gcagctcatc atcgatcagc agcagtttga cggcgctcgc
     5521 atcaagatcc gacatggcaa cacccgtgct cctggttctc gaaatcctac cgcttccacc
     5581 ctaccatcct catgccgcat caccaatttc tttctggctc tccgcaggga gtgatttcgg
     5641 ataccggagc gtggacagcg cctgccggag cgcccccatc gtcagcggct tgcttaattc
     5701 ggcatcgacc gcttgcgaca cggtgccatc cggctcgagt tcgtcgcccc cggtcaacag
     5761 cagcaccggt gtgtcgggag aaactttctt gatcacatcg gccaactgac ggcccgtcat
     5821 atgcggcatg ccgtgatcgg tcaccacgag atgcacccgc tcgggattga accgcctgat
     5881 cgcctccgcc gcactcccga ccacttctac gctatgcccg gccacccgga gatattcgcc
     5941 cgtcacgcgg cccaccaaag gatcgtcgtc tacgaccagg atctgtaagc ggaccccgtc
     6001 catgttgtgg ccgtcatgtt ctgtacctgt ctccgcctcc gcttgaatcg ggagccggat
     6061 gctgaacgtc gtccccacgc cgacggcact cgtgatatcg atggtgcctc gcaaccgccg
     6121 gatgatgccg tacaccatgg ccaatcccaa gccggacccc ttttcccctt tcgtggagaa
     6181 gaacggttca agacaccgtt gacgcacttc ttccgtcatc cccgtgccgg tgtcgctgac
     6241 ttccacgcgc accgcatcgc cgtcgagttt ggtacgcagt gtaatcgagc cgctcttcgg
     6301 catcgcatcg acggcgttga acactagatt cgtcagcact tcgcgcaatt cagactcatg
     6361 tccgcccact gcaggcaccg cctccaactc cgttttgatc tcgatcgcca cgccgttcgc
     6421 cagggcctga tccttccact tcggctgact aagcttcaca ctttgctgga ccaggctatt
     6481 cagatcgacg atgccggcca ggtccgcatc gaggcggggg cgatagaaat cccgcagccg
     6541 gctgacgacc ttcgccgcgt ccttggccgc catattgatc gtctgcagat attccttcag
     6601 ctgcgcgggg tccgccgcca tctggggcgc gatgagcagg agttcgctga aacccatgat
     6661 gggcgacagg gtattgttga agtcgtgcgc gatgccgctc gccatctggc cgagcgcccg
     6721 cagccgctcc tgctggacca tctgttgttg tgtcgtcttg agttcggtca tggcggcttc
     6781 caactgcgta tggctctccc gcaaggacgt ttccgcccgc ttccgctccg caagatccga
     6841 ggc
//
LOCUS       DAOQRM010000133         6822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_10999_length_6822_cov_20.313433, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000133 DAOQRM010000000
VERSION     DAOQRM010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10999_length_6822_cov_20.313433"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="PKK81_10195"
     CDS             <1..1671
                     /locus_tag="PKK81_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV33294.1"
                     /translation="FAALGLEASLLTTLEALGYEEPTPIQREAIPPLLEGRDLLGQAA
                     TGTGKTAAFALPLLQRIAHGPRQRPTALVLVPTRELAVQVSEAVQRYGKELRIGVLAL
                     YGGQAMGPQLQALRRGVEVIVATPGRALDHLRRKTLKLADLQVVVLDEADEMLDMGFA
                     DDLDAILEQTPASKQTALFSATMPPRIASIARRHLKNPVDVTIAREPVKAGAAPRVQQ
                     TAYVVARQHKVSALARVLDIATPKSALVFCRTRLEVDEVTAALNGRGYRAEAIHGGMS
                     QVQRDRVMQAFRSGQTELLVATDVAARGLDIPSVSHVINYDLPSSLEVYVHRIGRTGR
                     AGREGAAMTIIEPREQRLLRAVEQHTKAKITVAAVPSLGDLLAKRLERTKASIQETLE
                     AGELEDFRRVVEALSASAEPADIAAAAIKLVYRSHGGERAEEEIPAVSTRAPEPYRPS
                     RPAYGSAPQPGDRARAPRGGPSRGGRAAGTVRVYVGAGRAAGIRPGDLVGAIANEAGV
                     PSRMIGAIEVEERFSLVDVQEEAARQIIEALGRTRIKGQKVAVRLFRD"
     gene            2121..3683
                     /locus_tag="PKK81_10200"
     CDS             2121..3683
                     /locus_tag="PKK81_10200"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNV33295.1"
                     /translation="MAVTLAPWKAVLRDWQARAVADVLAQPREDYLVTATPAAGKTRF
                     ALRIAHQYLADRAASRVLVICPTNHLRTQWASAAGQVGIQLDPALSNEQACEARDYHG
                     AVVTYQQVCLAPEVFRRACRSRKTLVILDELHHAGDGKDWGKALRESFGEAVFRLALS
                     GTPFRSDNNPIPFVRYEQGESQADFTYGYSHSIRDGVCRPILFPSYEGELTWLSDGRE
                     HRATFEDGLTFERQRERLKTALLQETWLGPVLSDAHAELQRLRKQEQADAGGLIVTMN
                     QDHARQVADLVLKLTGTRALVAVSDDPSASKTIETFAHHKQQQWLVAVNMVSEGVDIP
                     RLRVGVYATNVLTEMYFRQVVGRFVRMQDKVPKPQRAWLYLPKDATLVHYAKRIKVER
                     DHVLEEIMPAVQRTLFGTAATSLKEYIPLNGVARLDALIGADEGDSAGDGKGQPEPAV
                     ALHDQKNTLRDKHRSLVGAVARKCGMDHRQLNAELIKRTGGRVDQATIPQLERRIALL
                     EKWRDSGFDKKR"
     gene            3766..4320
                     /locus_tag="PKK81_10205"
     CDS             3766..4320
                     /locus_tag="PKK81_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV33296.1"
                     /translation="MAGKQVARRTSAVAKKITKQAVKKSGTKKAAPARRAKTPRAKRP
                     SVAVVVLSGSTPLRRNKAAELVAEELQLDLAKVNLASVVSRQVGETEKNINRVFDEAE
                     RSGSILFFDGADALFGTGRAGKAGTSHSTAAGAAHLLQRIEDHNGLVILTTNGKSRID
                     QALSRQAKVSVMVGIKKKPKKKTA"
     gene            complement(4496..4666)
                     /locus_tag="PKK81_10210"
     CDS             complement(4496..4666)
                     /locus_tag="PKK81_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33297.1"
                     /translation="MPKARTAKNCYYCEAEDRIKMTFMLCGLCHRHFCSAHGVPDLEQ
                     CTKCLEASEETE"
     gene            complement(5149..6777)
                     /locus_tag="PKK81_10215"
     CDS             complement(5149..6777)
                     /locus_tag="PKK81_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNV33298.1"
                     /translation="MSLPVEKSAADELRREVLRRRTFAIISHPDAGKTTLTEKLLLYA
                     GAVHLAGAVQARSSQRQAKSDWMELEQARGISITSTMLQFDYQGARVNLLDTPGHQDF
                     SEDTYRTLMAVDSAVMVLDGAKGIEPQTKKLFAVCRKRGIPILTFINKMDQPGRHPFD
                     LLDEIERTLGMTAVPFNWPIGEGSGFQGLYDFQHRQVLFFQRTAHNQRRAPMTVEAFP
                     NPKLAETLGDAYGPLQEEIGLLTGAGTSFDRDRFLAGELTPVFFGSALTNFGVGPFLD
                     AFTKLAPPPGPRQTAQGLIQPTDEAFSGFVFKIQANMDPQHRDRMAFLRICSGRFEKD
                     MMVHHARLGRKIRMTRPHRLFGRDRETIDEAYPGDVVGLVNPGLFTIGDTLTSDPALT
                     FDPIPHFAPECFGLLRTQDISKHKQFQKGLKQLEEEGVMQIFYSPDHVRREPVLAAVG
                     ELQFDVVMSRLENEYGVKTSVERLPHALARWIVGDPATISAVYWPSDTIRLVDQQGRG
                     VMLFTTEHLLAYCIKSNPSIKFLLREEVEQTSAQ"
BASE COUNT         1267 a         1950 c         2302 g         1303 t
ORIGIN      
        1 tttgccgcgc tcggattaga agcctcgctg ttgaccaccc tggaagcctt gggctacgaa
       61 gagcccaccc cgattcagcg cgaagccatc cctccgcttc tggagggacg cgacctgctg
      121 gggcaggccg ccacgggaac cggaaagaca gcggcgtttg cgttgccgct cctgcagcgg
      181 atcgcccatg ggccacggca gcggcccacg gcattggtgc tggtaccgac cagggaactc
      241 gccgtgcagg tgagtgaggc ggtgcagcgg tacgggaagg agctgcggat cggggtgctc
      301 gccctgtacg gcggtcaggc gatgggaccg caactgcagg cgctccggcg cggggttgaa
      361 gtcatcgtgg cgacgccggg ccgggccttg gatcacctcc gccgcaagac cttgaagctg
      421 gccgatctcc aagtggtggt gctcgatgag gcggacgaaa tgctcgacat gggcttcgcc
      481 gacgacctcg atgccattct cgaacaaact ccggccagca agcagaccgc gttgttctcg
      541 gcgaccatgc cgccgcggat tgcgtccatc gcccgccggc atttgaagaa tccggtggat
      601 gtgacgatcg cccgtgagcc ggtgaaggca ggcgccgcgc cgcgcgtgca gcagaccgcc
      661 tatgtggtgg cgcgacagca taaggtgtcc gcgctggccc gcgtgttgga tattgcgacg
      721 cccaaatcgg cactcgtgtt ttgccggacg cggttggaag tggatgaagt gacggcggcg
      781 ctgaacggtc ggggttatcg tgccgaggcc attcatggcg gcatgagcca ggtgcagcgg
      841 gatcgcgtga tgcaggcgtt tcgctcagga caaaccgaac tcctcgtggc gaccgacgtg
      901 gccgcgcgcg ggttggatat tccatccgtc tcccacgtga ttaattacga cctgccctcg
      961 tcgttggaag tctatgtcca tcggatcggg cgaacgggcc gggcggggcg cgaaggcgcg
     1021 gcgatgacta tcatcgagcc gcgtgaacag cggttgttgc gcgcggtcga acagcatacc
     1081 aaagcgaaaa ttacggtcgc tgctgtgcca tccctgggtg atctgttggc caaacggctg
     1141 gagcggacca aggcctccat tcaagaaacc ttggaggccg gcgagctcga agattttcgt
     1201 cgtgtcgtgg aagcgctgtc ggcgtcggca gagccggcgg atatcgcggc ggcggccatc
     1261 aagctggtct accgatccca tggcggtgaa cgggcggaag aagaaatccc ggcagtctcg
     1321 accagggcgc cggagcccta ccggccgtcg cgtcctgcgt acggttcggc tcctcagccc
     1381 ggggatcgcg cgcgtgcgcc ccgcggtggt ccgagtcgcg gtggtcgggc agccggaacc
     1441 gtgcgggtct atgtcggggc ggggcgggcg gcaggtatca ggcctggcga tctcgtcggt
     1501 gccatcgcca atgaagcggg cgtgccgtcg cgtatgatcg gggcgatcga agtcgaggaa
     1561 cgattttccc tggtggatgt gcaggaagag gcggcccggc agattatcga ggccctgggg
     1621 cggacgcgca tcaaggggca aaaagtcgcc gtgagattgt tcagagattg agcaacgagg
     1681 cgggccggcc gttgacggac gtcacggccg gctcaccgcg tgatcctccg gagtgtgtct
     1741 tccgtcaacc gttcccaccg tcgagtccgg ccggtctatc cccccgcgca ggctgagcca
     1801 tcaccaccgt ttcggcccgg catcttccgt cttggccctt gttgcctgac cggttttgcg
     1861 ataccatccc gcacagacat gatgcgtctc acccataccg tgccggccgc ctcgcgatcg
     1921 tgagggtgtg ccggcagagt gtgcgtcaca cctcattacc agatcagttc caggcagaca
     1981 gtctctgtgg tgcctcgcac cggcgagcca ttacggaacg ggactggcgg cgtctgacat
     2041 ttggtggcga tccaggggga tggcgctggg gtgagactga gttccaatga gtcacggcga
     2101 cggagaggat ctgtgttgag atggcggtga cgctcgcacc atggaaagcc gtgctgcggg
     2161 actggcaggc gagggcggtc gccgatgtgt tggcgcagcc gcgcgaggac tacctcgtca
     2221 ccgcgacgcc tgcggccggg aaaacacgct tcgcgctccg gatcgcccat cagtatctcg
     2281 ccgatcgggc cgcgagtcgt gtcctggtga tctgcccgac gaatcatctt cgaacgcagt
     2341 gggcttccgc ggcggggcag gtcggcattc agctcgatcc ggcgctctcg aatgagcagg
     2401 cctgcgaggc gcgtgactat cacggcgcgg ttgtgaccta tcagcaagtc tgtctggcgc
     2461 cggaagtgtt ccggcgcgcc tgccgcagtc gcaaaactct cgtcatcctc gatgagttgc
     2521 accatgccgg tgacggtaag gattggggca aagcgctgcg cgaatccttc ggcgaagcgg
     2581 tctttcgtct ggcgctgtcc ggcactccct tccgctcgga taataacccg attcccttcg
     2641 tgcgatatga gcagggcgaa agccaggcgg atttcaccta tgggtattcg cattcgattc
     2701 gtgacggggt ctgtcgcccg attctgttcc cgagttacga aggcgaactg acctggttgt
     2761 cggacgggcg tgagcatcgg gccaccttcg aagacgggtt aaccttcgaa cgccagcggg
     2821 aacggctcaa gaccgcgttg cttcaggaga catggctggg cccggtcttg agcgacgccc
     2881 atgccgagtt gcagcggttg cgcaagcagg aacaggcgga tgcgggcggg ttgatcgtga
     2941 ccatgaatca ggaccatgcg cgacaggtgg cggacctggt tctgaagctc acgggcacac
     3001 gcgcgctggt ggcggtgtcc gacgatccat ccgcctcgaa gaccatcgaa acgtttgcgc
     3061 atcacaagca gcagcagtgg ctggtggcgg tgaatatggt gagcgaaggc gtcgatattc
     3121 ctcgcctgcg ggtcggtgtg tatgcgacca acgtgttgac cgagatgtat ttccgccagg
     3181 tcgtcgggcg gtttgtgcgg atgcaggata aggtgccgaa accgcaacgg gcctggctct
     3241 atctgccgaa ggatgccacg ctggtgcatt atgccaagcg catcaaggtc gagcgcgatc
     3301 acgtcctcga agagatcatg ccggcggtgc aacggacact cttcggcacg gcggccacct
     3361 cgctcaagga gtacattccc ctcaatggcg tggccaggct cgatgccttg atcggtgcgg
     3421 acgagggtga ttcggcgggt gacggcaagg ggcagccgga accggcggtc gcgttgcatg
     3481 accagaaaaa caccttgcgg gacaaacatc gctctctggt cggggcggtg gcgcggaagt
     3541 gcgggatgga tcatcggcag ctgaatgcgg agttgatcaa gcggacaggc ggccgggtcg
     3601 atcaggcgac catcccgcaa ctggagcgac ggatcgccct gctggaaaag tggcgtgatt
     3661 ccggcttcga caagaagcgc tgagcggctg aagtgtggta atgatggcag gctccatcgg
     3721 cggatgtgcg atttcgttga atattgatgt gagaggaagg caaccatggc tgggaaacaa
     3781 gtggcgcgcc ggacgagtgc ggtcgccaag aagatcacga agcaggcggt gaaaaaatca
     3841 ggaacgaaaa aagcggcgcc tgcgcgccgc gcaaagacgc ctcgcgccaa gcggccgtcc
     3901 gtggcggtgg tggtgctgtc cggttccact ccgcttcgcc ggaacaaggc ggcggagttg
     3961 gtggcggaag aactgcaatt ggatctggca aaggtgaacc tggcttcggt ggtcagccgt
     4021 caggtcggtg agacggagaa gaacatcaac cgggtgttcg atgaggcgga acggagcgga
     4081 tcgattctct tcttcgacgg cgcggatgcg ctgttcggca cgggccgggc gggcaaggcg
     4141 ggcaccagtc actcgaccgc tgccggggcg gcccatctgc tacaacgcat cgaggatcat
     4201 aacggcctgg tcattctcac gaccaacggg aaaagccgga tcgatcaagc cttgtcgcgg
     4261 caagcgaagg tgtccgtgat ggtgggcatc aagaagaagc caaagaagaa gacggcctga
     4321 cgagtcgttc tctcaatggt tgtttgagaa gcgatgtcac gagtacagga tgctcaaaaa
     4381 ggctgttcag caaggccgca gcgagcgagg gggccgaggc gtaccctcgg ggtacgttga
     4441 gggtccgagc gatgcgagaa cgcggctgac agactttttc agcatcctgt gaggattatt
     4501 ccgtttcttc gctggcctcc agacacttcg tgcactgttc cagatcgggc acgccatggg
     4561 cgctgcagaa gtgccgatgg cagaggccgc agagcataaa ggtcatcttg atgcggtctt
     4621 ccgcttcgca gtagtaacag ttttttgcgg ttcttgcctt cggcatggtc cttctcctcg
     4681 aaacatgtgt cggttcaatt accagtcatt gagtgagtgc acgagtcgca ttccgtcggc
     4741 gtcagtgcgc ttcgccgttc catgccgatg atcgggcatg agggcggcag gacctgaaac
     4801 catgacaggg ggcgggcaag gcacctaggc cgagttagcc tgaggcgcta agggcagcta
     4861 gaggtgtgat cgtgctcgcg ttcaccattc cacagggtcg gccggcactc ccgtggactg
     4921 atgctcctta taacacattt tgccgatcgt cggcttggcc ctgaccgggc ggccgccggc
     4981 ggcgttctgc acatctgtga atcgtgaagc gtatctcgtc agggacgccc actctcgatt
     5041 tccggccatg tgcgatgaac tctatgccat acgctctttt ctgcggacga gagacgaacg
     5101 acgtttcacg aacgacggac taggaggacg gcctttttgc gcatcctgct attgtgccga
     5161 ggtctgctcg acttcttccc tcaacagaaa cttgatcgac gggttcgatt tgatgcaata
     5221 ggccagcagg tgttcggtgg tgaacaacat gacgccgcgg ccttgctggt cgacgagccg
     5281 gatggtgtcg gacggccagt agacggccga aatcgtggcg ggatcaccga cgatccagcg
     5341 ggccagcgca tggggcaggc gctccacgga tgtctttacc ccatactcat tctccaggcg
     5401 agacatcacg acgtcgaact gcaattcacc caccgcggcc agcaccggtt cgcggcgcac
     5461 gtggtccggt gaataaaaga tctgcatgac cccttcttct tccagctgct tgagcccctt
     5521 ctgaaactgc ttgtgcttcg aaatatcctg tgtgcgcaac aggccgaaac attccggcgc
     5581 aaagtgcgga atcggatcga aggtcagcgc gggatcggag gtcagggtat cgccgatcgt
     5641 gaacaggccg gggtttacca acccgacgac atcgccgggg taggcttcgt cgatcgtttc
     5701 gcggtcgcgg ccgaacaggc gatgggggcg tgtcatccgg atcttgcggc ccagcctggc
     5761 gtgatggacc atcatgtctt tttcgaagcg gccggagcag atgcggagga aggccatgcg
     5821 gtcgcgatgc tgcgggtcca tgttcgcctg aatcttgaac acgaagccgg agaacgcttc
     5881 gtcggtcggt tgtatcaatc cctgggccgt ctggcgcggt ccgggaggcg gcgctagttt
     5941 ggtaaaggca tccaggaacg gcccgacgcc gaagttggtc aacgcgctgc cgaagaagac
     6001 cggcgtcagt tcacccgcga ggaaccggtc gcgatcgaag gatgtgcctg cccccgtcaa
     6061 cagtccgatc tcttcctgca ggggcccgta ggcatcgccg agggtttccg ccagtttcgg
     6121 attcggaaag gcctcgaccg tcatcggcgc gcgccgttga ttgtgtgccg tacgctggaa
     6181 gaacagcacc tggcggtgtt ggaaatcgta caggccttga aagcctgacc cttcgccgat
     6241 cggccaattg aagggcaccg ccgtcatccc tagggttcgc tcgatttcat ccagcaggtc
     6301 gaatggatgg cgtcccggct ggtccatctt gttgatgaag gtcaggatcg ggatgccacg
     6361 cttgcggcag acggcgaaga gtttcttggt ctgcggctcg atacctttgg cgccgtccag
     6421 caccatgacg gcgctgtcca cggccatgag tgtgcgatat gtgtcttcgc tgaagtcctg
     6481 gtggcctggc gtgtcgagca ggttgacccg cgcgccctga tagtcgaact gcagcatggt
     6541 ggaggtaatc gaaatgccgc gcgcctgctc caattccatc cagtcggact tggcttgacg
     6601 ctggctggag cgcgcttgca ccgcgccggc caggtgcacc gcgccggcat agagcagcag
     6661 cttttcggtc aaggtggttt ttccggcgtc cggatgggaa atgatcgcaa acgtgcgccg
     6721 tcgcagcact tcgcgccgca attcatcggc cgcagatttc tcaaccggca gggacatagg
     6781 tcttctcttt gtggttaggg gaagccaacg ggacagggga tc
//
LOCUS       DAOQRM010000134         6798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_11053_length_6798_cov_23.996144, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000134 DAOQRM010000000
VERSION     DAOQRM010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11053_length_6798_cov_23.996144"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..217
                     /locus_tag="PKK81_10220"
                     /pseudo
     CDS             1..217
                     /locus_tag="PKK81_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611536.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(260..1087)
                     /locus_tag="PKK81_10225"
     CDS             complement(260..1087)
                     /locus_tag="PKK81_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33299.1"
                     /translation="MSLLRVVQSPEEQTVFSHSKVLAGFVTETNIGELRGSRLVFRAD
                     DKEKIRAWLRADRIDPMTPPASFGTMSRAEAIGAGPDEKWAGKQVRTGKVAIKTRPGR
                     PLLLDGREIWLPAKANVELSIDDSDTARMHQSVVVVENWLCFEEIGQLQINWSRAGEN
                     PLIVWRGGDQVLRADATMEFLQRLGAPVWSAPDYDPAGLAIAARLPELAGVLMPNLDV
                     LRELLGNSRLADRYRIQLPGAARVLDEATNQDIALLWDLIRQEGRALPQEKLLRLLP"
     gene            complement(1114..2346)
                     /locus_tag="PKK81_10230"
     CDS             complement(1114..2346)
                     /locus_tag="PKK81_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33300.1"
                     /translation="MSDKLDLVTAYLSKLFKHKELVAAAYHQGVVAKGDDRYDGRGLY
                     ELHQARTLVPYAQDTYRLGSSLAKHFDEVLQKEQLYAAVGANVGDLAHRLPLLADAVA
                     RANLEGRIDDADLYTDQFDRGVFELADSISGALQLLRVMADNKFSNVSTLAEKRRQNE
                     FYIERAKIIGDALLLLQANGLMEILEGVPNGERLIFAYRSQLDDQLPEWRSSLLDITA
                     ILKAYLFRLRQVEPAARRFRAFALFLKRTPDYVPPEIDEIAEPPSWACKAAGYRLKSS
                     AVVTSAENSEILVDIARSILPAKQQLIPVPRVGRLELDLNPPAEDVVEIKPWQAGLQE
                     LLRTATQAPASVIEWKRNRPYLAAVPNDIWLLCILHEQSLKLQRSKGIQFEDVIVAGP
                     FLSGCLSIRDILISRATP"
     gene            complement(2352..2972)
                     /locus_tag="PKK81_10235"
     CDS             complement(2352..2972)
                     /locus_tag="PKK81_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33301.1"
                     /translation="MFEKTVTLLLSGEFVCEVCYPEAYGFLDNEAHRKDMTAFLARLG
                     RRLSVTGLGGAWFMAYEHIGPEERRAIKDEFSFMKHELRPLVDFFVLAMRALQHEEFF
                     SRGSIIEANRLMGAIDANPSLRADFQSLSSMGKGSVGDGTLRSALERQLKRLRENGYL
                     VLANPEREIYRVTGKIEYLQEVVEFLMSHEGVVEELDDEPENASLF"
     gene            complement(2965..6654)
                     /locus_tag="PKK81_10240"
     CDS             complement(2965..6654)
                     /locus_tag="PKK81_10240"
                     /inference="COORDINATES: protein motif:HMM:NF023550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV33302.1"
                     /translation="MTTTDVLLAETATCEFELLRLVMVDSYTKGKITELPISGGTIIT
                     GRNGRGKTSLLQIILAFYGERPDRIVVPASNKVSFARYYLPRMTSYLVYEYRRRDVIC
                     CAVLYSDDTGDALRYRFIRSPYRQEFFVQPDLATIVMSRDLTAHLKQLGANTSRPMDL
                     YEYRNIIQGRSGTGRDAHKQRAMVADFSFCPSGRQIAHIERIVSGMFLRRTNFTDLQR
                     MVVASIAESANQIALGADRKRIDVWPDHYESYMSVMAQEPRMAAASSSYDTLLFEEQE
                     IGRIHARLTLLVAKLQGDLTQALERRASAKRNIEAEETQYRDRARVFDEAIERAERAA
                     QTADAEVRALDKRRDHYKQIDVDAKAALLGRESEIRHSKEALERRQQLLTGTAQNIAT
                     EYDRLLNEMNRLRLLDTAAASVARGEIIQSFIPRLAALESARDNEYRDLRTRAEGQLR
                     SARDAVEAAQRAIGEAQAKVSNPQADNLTVKALEDKLVELKKLRTSLADERKQLKSRR
                     DAARFAEKAYAREEQSRARLIQERNRLTTVLEELLLRQAPSEDSLLYFLRTQLPGWHH
                     DLAKVIREDLLVRTDLSPSIGSNSGGMYGLELDVDQLDAHLAADETALKEEILAATGR
                     IKAIEVDIAAADLLLDTAGAARHAALADEELQERKVGGIEQTIATKENEEVAARSLVE
                     KSKASARTVAESTFAEAKEQLGTAKESVVVLETALQSALELRQRRYAKERAELAAEQA
                     VATKVIDDAETGAARAHEARCQSIAQERTQKLSAAGVDVKALASLSDQLKQAFEEIAL
                     IESSRNLVAEWRLWLDKDWSRRSLLEKESVDARAIARARRDDKTLLQNAWDKRLPALK
                     TEESQAVAQVQKLELEIGQIEKRRFLLDNYPPDEPTLGERVDPTWTLTSLTQALNQHQ
                     RAIQDTEKALALAIASLKTAFVAHRGTPPDDFYISHRQQLGADESERARAWVPIFRSW
                     YSGEHEAFRNTLRVEAAQIAGAISSFHQQMDRFHKQVVRFNASLQENLDANLGFESIS
                     RVTVKIVSTVHQLEYWPVIEKVANSQRIWIAGDGTDLPPPEFSATLRELLEHWEVRAG
                     IRAELTHQISIQGEVIENGNLRTFRKAEDLETVSSNGLSYIVLCVIFIGFINRIRRGA
                     AINVTWALDEIKDLDIGNVEVLMSVLRKNNITLVSACPDPDVDVLAMFKNRRSIRSDR
                     VIYDPSGALAHRLTAPVGDEVLSDV"
BASE COUNT         1325 a         2076 c         1911 g         1486 t
ORIGIN      
        1 gacaattcat gctctcgcga cttttccgcg cttcaacccc gcgccggaca gcaggtcgag
       61 agcgagtgcc cgatgcaaca acgttgctcc actttcgcca cttgtatcac cgaggcgcag
      121 ggttttttgc gaaagtcgtc gagttgctgt tggctaacgg gcgctttcgg gcggcacgag
      181 tgtcgatgcc atgttgatcg aggcaccgcc ctcgacctag aaccgagccg gacaaaaaaa
      241 cagaatccga gaccttcggt tacggtaaca accgtaatag cttttcttgg ggcagcgcgc
      301 gaccttcttg ccggatcagg tcccagagca atgcaatgtc ctggttcgtt gcttcatcca
      361 acacgcgcgc tgccccaggg agctgaattc gatatcgatc ggcaagccgg ctgttcccca
      421 gcagctctcg caatacgtcg agattcggca tgagaacacc ggccagctca ggcaatcgtg
      481 ctgcaatcgc gaggccggca ggatcatagt ccggcgccga ccagacggga gcaccgagcc
      541 gctggagaaa ctccatggtg gcatcggcgc gcaatacctg gtcaccgccc cgccaaacga
      601 tcagcgggtt ctcccctgct ctgctccaat tgatttgtaa ctgcccgatc tcctcgaagc
      661 acagccagtt ctcgaccacg accacgctct gatgcatcct cgcggtgtcc gagtcatcga
      721 tggacaactc gacgtttgct ttcgctggca gccaaatttc ccgaccatcc aacagcaatg
      781 gccggccggg gcgcgtcttg atggccactt tccctgttcg gacttgcttt cccgcccact
      841 tctcgtcggg tcccgcgcca attgcctcgg cacgcgacat ggtgccgaag gacgctggtg
      901 gcgtcatggg atcaatgcgg tcggcacgca gccaggcgcg tatcttttcc ttgtcgtccg
      961 cccgaaaaac cagccggctg cctcgcagtt ctccgatgtt tgtttccgtc acgaagccgg
     1021 caaggacctt ggagtgcgaa aaaacggttt gctcctccgg gctttgcacc acccgcagca
     1081 ggctcaacgc cagctgcttc cccgttgctc ccgtcatggg gtggctcgtg atatcagaat
     1141 gtcccgaatc gacaagcagc cggagagaaa ggggcccgcc acgatgacat cctcaaactg
     1201 gatccccttg gatcgctgga gtttcagaga ctgctcatgg agaatgcaga gcagccagat
     1261 gtcattcggc acggcggcca gataggggcg atttcgtttc cattcgatca ccgacgccgg
     1321 cgcctgcgtc gctgtccgca gcaattcctg aagcccggct tgccatggct tgatctcgac
     1381 cacgtcctcc gccgggggat tcaaatcaag ctccaggcga ccgacgcgtg gcacggggat
     1441 cagctgttgc ttggcgggca gaatggaccg ggcgatatcg accaggattt cgctgttttc
     1501 agccgaagta accactgccg acgatttcaa gcggtagccg gccgctttgc aggcccaaga
     1561 cggcggttcg gcgatttcgt cgatctccgg cgggacataa tccggcgtgc gcttcagaaa
     1621 gagggcaaag gctctgaagc gtcgcgccgc tggctcgacc tggcgcaagc ggaacaggta
     1681 ggccttaaga atcgcagtga tatcgagcag cgacgatcgc cactccggaa gctggtcgtc
     1741 cagctgggac cggtaagcga agatcagacg ctcgccatta ggaacgcctt ccagaatttc
     1801 catcagccca ttcgcctgca acagcagcag cgcgtcaccg atgatcttgg cccgttcgat
     1861 gtaaaactcg ttctgccgcc ttttctcagc cagggtgctg acgttcgaaa acttgttatc
     1921 cgccatgacc cggagcagtt gcagggcacc ggaaatgcta tcggcaagct cgaagacgcc
     1981 ccggtcaaat tgatccgtgt agagatccgc gtcgtcgatc cgtccttcga ggtttgctct
     2041 agccaccgca tcggccagca atggcaaacg gtgggccaag tcacccacgt tggccccaac
     2101 cgcggcgtag agctgttcct tttgaagcac ttcgtcgaag tgttttgcca ggctggaccc
     2161 gagtcggtag gtgtcctggg cataggggac cagggtgcgc gcctgatgca gttcgtagag
     2221 gcctcgcccg tcgtaacgat catcgccctt ggcaaccacg ccttgatgat aggcggccgc
     2281 cactaattcc ttatgcttga atagcttgga gagataggca gtaacgagat ccagtttgtc
     2341 ggacatggcg atcagaacag gctcgcgttt tcgggctcgt catcaagttc ttcgacgaca
     2401 ccctcgtggc tcatcagaaa ctcgaccacc tcctggaggt actcaatctt tccggtaact
     2461 cggtagatct cgcgctcggg attggcaagg acgagatagc cattctccct aagccgcttc
     2521 aactgtcgtt cgagcgcact gcgcagggtg ccgtcaccaa cacttccttt ccccatgctg
     2581 gaaagggact ggaagtcggc tcgcaggctg ggattggcat caatcgcgcc catcaggcga
     2641 ttagcctcga tgatcgatcc gcgcgagaaa aattcctcgt gctgcaacgc ccgcatggcc
     2701 agcacgaaga aatcgaccaa gggacgcagt tcgtgcttca tgaacgagaa ttcgtccttg
     2761 atcgcccggc gctcttccgg tccgatgtgc tcgtaggcca tgaaccacgc tccgccaagc
     2821 cccgtcaccg acagccgacg gccgaggcgg gccagaaaag ccgtcatgtc tttccgatga
     2881 gcttcgttat cgagaaagcc gtaagcctcc ggatagcata cttcgcagac aaactcaccg
     2941 gacaatagga gggtgaccgt tttctcaaac atcactgagc acctcgtcgc caactggcgc
     3001 cgttagcctg tgcgccaacg cgccgctagg gtcgtagatc acgcggtcgg atcgaatgga
     3061 gcggcggttt ttgaacatgg ccagaacatc cacatcgggg tccgggcacg ccgaaaccag
     3121 ggtgatgttg ttctttcgca gaacgctcat caatacctca acattgccga tatccagatc
     3181 cttgatttca tcgagcgccc aggtgacgtt gatcgccgcc cctcgtcgaa tgcgattgat
     3241 gaaaccgatg aaaatcacgc aaaggacgat ataggagagc ccattggatg acaccgtttc
     3301 gagatcctcg gcctttcgga acgtgcgcag gttgccattc tcgatcactt cgccctggat
     3361 gctgatctgg tgggtcagtt cagcacgaat gcccgcgcgg acctcccaat gctcgagtaa
     3421 ctcccgtaac gtcgcagaaa attctggcgg cggcagatcg gtgccgtcgc cggcaatcca
     3481 gatccgctgt gagttggcga ctttctcgat gactggccag tattccagct gatgaacggt
     3541 tgaaacaatc ttcaccgtca ctcgggaaat gctctcaaag ccgaggtttg cgtcgaggtt
     3601 ctcctgcaag ctggcgttga accggaccac ctgtttgtgg aaacggtcca tctgctgatg
     3661 gaaactggag atagcgcccg cgatctgcgc cgcctcgacc cgcagggtgt tccggaaggc
     3721 ctcgtgctcg ccgctgtacc aactgcggaa gataggcacc caggcacggg cacgttccga
     3781 ttcgtcggcg ccaagttgtt gccggtggga aatgtagaaa tcgtcgggcg gcgtacctcg
     3841 gtgcgcgacg aaagcggttt ttagcgatgc aatagccaac gccagcgcct tctcggtatc
     3901 ctgaatggcc cgttggtgct gattcagggc ctgggtaagc gaggtcagtg tccacgtggg
     3961 atcgaccctt tcccccaagg tcggctcgtc tggcgggtag ttgtcgagga gaaagcgccg
     4021 cttctcgatc tggccaatct ccaattccaa cttctgcacc tgggcaacgg cttgggactc
     4081 ttccgttttc agcgccggca agcgcttatc ccacgcgttc tggagaagcg ttttgtcgtc
     4141 acgccttgcc cgcgcaatgg cccgcgcgtc gaccgattct ttctccagaa gagaacgcct
     4201 cgaccaatcc ttgtccagcc aaaggcgcca ttcggccacc aagttacgcg acgactcgat
     4261 gagtgcaatt tcctcgaacg cctgcttgag ctgatcactg agggacgcca gcgccttcac
     4321 gtctacgccg gccgcgctca gtttctgcgt tcgctcctgg gcaatgctct ggcaacgcgc
     4381 ttcatgcgcc cgcgctgcac cggtctcggc gtcatcgatg acttttgtgg ctaccgcttg
     4441 ttcggctgca agctcggcgc gctccttcgc ataccgccgc tgccgcagtt cgagagcgct
     4501 ctggagtgcc gtctccagga caacgaccga ttccttggcc gtgcccagtt gctctttggc
     4561 ttcagcaaat gtgctttcgg ccaccgttct tgcggatgcc ttgctcttct ccaccaggct
     4621 tctggccgca acctcttcat tttccttcgt ggcgatcgtt tgctcgatgc cgccgacctt
     4681 gcgctcctga agctcctcgt ccgccaaggc agcatggcga gcggcacccg cggtatcgag
     4741 caggaggtca gccgcggcaa tgtcgacttc tatcgctttg atccgtcccg tggcggcgag
     4801 gatttcctcc tttagcgcag tctcatcggc ggccaggtga gcgtccagct gatcgacatc
     4861 gagttccagg ccgtacatcc cgccggaatt gctcccgata gacggggaga gatcggttct
     4921 gaccagcaga tcttcccgga tgaccttggc gagatcatgg tgccaaccag gcaactgcgt
     4981 gcgcaggaaa tacagcagac tgtcctcgct cggtgcctga cgaagcagca gttcctcgag
     5041 taccgtggtg agcctgttcc gctcctgaat cagacgtgcc cgcgactgct cttcccttgc
     5101 gtaggctttt tcggcgaacc tggctgcgtc ccgtcgcgat ttgagctgct ttctttcgtc
     5161 agcgagggaa gtgcgcaact tcttcagctc gacgagcttg tcctccaacg ctttgacggt
     5221 caggttgtcc gcctgcgggt tagacacttt tgcctgagct tcgccgattg cacgctgcgc
     5281 tgcttctacg gcatcgcgcg ccgaccgcaa ctgtccctcg gcgcgggtcc gaaggtcacg
     5341 gtattcattg tcccgcgccg attcaagggc agccaagcgc ggaatgaacg attgaatgat
     5401 ttcccccctt gcaacgctcg cggcggccgt atccagcagg cgcaggcgat tcatttcgtt
     5461 cagcaggcga tcgtactcgg tggcgatatt ttgtgccgtc ccggtcaaga gctgctgacg
     5521 tctttcgagc gcctctttac tgtggcgaat ctcgctttcc cgaccgagca acgcggcctt
     5581 ggcatcgacg tcaatttgct tgtagtgatc ccgacgctta tcgagtgctc ggacttctgc
     5641 atcggctgtt tgcgcggcgc gctctgctcg ctcgatggct tcatcaaaaa cgcgggcacg
     5701 gtctctgtac tgggtctctt ctgcctcaat gtttcgcttt gcgctcgcac gccgttcgag
     5761 tgcctgggtg aggtcaccct ggagctttgc cacgagcagt gtcagtcgcg catggatccg
     5821 gccgatttcc tgctcctcaa acaacagcgt gtcgtagctg ctcgatgcag ccgccatccg
     5881 cggttcctgg gccatgaccg acatgtacga ctcgtaatga tccggccaga catcgatcct
     5941 tttcctatcc gcccccaacg ctatctggtt ggctgactcg gcaatcgacg cgaccaccat
     6001 gcgttgcagg tcggtgaagt tcgttctgcg caggaacatc ccgctgacaa tgcgttcgat
     6061 atgggctatc tgccgaccac tcggacagaa cgagaagtct gcgaccatcg cgcgctgctt
     6121 gtgcgcgtca cgaccggtgc cgctacgccc ttgaatgatg ttccgatatt cgtacagatc
     6181 catggggcgg ctggtgttgg cacccagttg cttcagatgc gccgtcagat ctcgactcat
     6241 cacgatcgtt gccagatccg gctggacaaa gaattcctgt cgatacggac tgcggataaa
     6301 acgatagcgc agggcatccc cggtatcgtc gctgtatagg acagcacagc agatgacatc
     6361 gcgacgtcgg tactcgtaca cgaggtagga ggtcatccga ggcaggtagt agcgcgcgaa
     6421 actgaccttg ttgctcgcag gcacaactat ccgatctgga cgctctccat aaaaagcgag
     6481 gattatctgt aggagcgagg tttttcctct gccattccgg cccgtgatga tcgttccacc
     6541 ggaaatgggc agttccgtga ttttgccttt ggtgtaggaa tccaccatca cgagccggag
     6601 caattcaaac tcgcaggtgg ccgtttcagc cagcaggaca tctgttgttg tcataaatcc
     6661 aatgctgtaa tttggcattc aaaatgaatg ccggagaccg acttgtaaaa ctgattaggc
     6721 gatggtggca attagctact ccacaccaat cgccttgaac accgcatcca ccggatccga
     6781 gtagaagctg gtctgaaa
//
LOCUS       DAOQRM010000135         6689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_11334_length_6689_cov_23.665662, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000135 DAOQRM010000000
VERSION     DAOQRM010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11334_length_6689_cov_23.665662"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKK81_10245"
     CDS             <1..447
                     /locus_tag="PKK81_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV33303.1"
                     /translation="IEADRDVYTRHVVERLQTKGVVVASENWEQKNTLPLPAQFLMES
                     GRYIAKKGLGIQYRLISLWPINKRNVAANELEKAGLGTILTQPNRPHTGFMKIGETRY
                     FQAVYADLAATQACLGCHNAHPDSPKRDFKLNDVMGAIVITIPVGQ"
     gene            complement(566..1225)
                     /locus_tag="PKK81_10250"
     CDS             complement(566..1225)
                     /locus_tag="PKK81_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33304.1"
                     /translation="MDNARPPRSVFSLEEINLLMPTVRKSFQEATSREWIVFMLVGQE
                     GTGRETTSGGMFVEEGKLHLVIANHRLALAENSEELARVRANPLHSVQGSGGALVFDL
                     PRFVIGTKANWSGGHRASASELILDHAAFLSSLQHRGSAAAPVPVSGSHAPVSAPEAD
                     APGPGSFHSGQVDSEQIILQLREEIEVLKQQLADKDAEIDRLKPREIRTTPAPVIRTP
                     P"
     gene            complement(1537..1959)
                     /locus_tag="PKK81_10255"
     CDS             complement(1537..1959)
                     /locus_tag="PKK81_10255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNV33305.1"
                     /translation="MAGKILLEVVTPEKLLLSQEVDEVIAPGSEGEFGVLPGHCHFLS
                     SLRIGELRYKTQDAWHYMSILWGYAEVTPSKVTVMAEIAEKAEDIDVGRAQQAVEKAE
                     QRLQAGGLPSEVKEAQISLEKARLRKKIADRAKKSGHA"
     gene            complement(2085..3527)
                     /gene="atpD"
                     /locus_tag="PKK81_10260"
     CDS             complement(2085..3527)
                     /gene="atpD"
                     /locus_tag="PKK81_10260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNV33306.1"
                     /translation="MSTGKVIQVIGPVVDVEFPPGQLPNIYNALKVTQEENKAAGKPA
                     VRITLEVASHLGENRVRGIAMSTTDGLTRGMDVQDTGAPISVPVGRETLGRLINVLGE
                     PVDEKGPIKAKKTYPIHRPAPRLEDQDTKTEVLETGIKVVDLLEPYSKGGKVGLFGGA
                     GVGKTVIIMELINNIALHHGGFSVFAGVGERTREGNDLWHEMQESKVIDPDDHTKSKA
                     ALVYGQMNEPPGARLRVALTGLAVAEFFRDEENQDVLLFVDNIFRFTQAGSEVSALLG
                     RMPSAVGYQPNLSTEMGALQERITSTKKGSITSVQAIYVPADDLTDPAPATAFAHLDA
                     TTVLSRQLAELGIYPAVDPLDSTSRILDPQVIGEEHYKVARGVQSVLQRYKDLQDIIA
                     ILGMDELSEDDKMAVARARKIQRFLSQPFHVAEAFTGAPGKYVKLKDTVRSFKEILEG
                     KYDHLPEQAFYMVGPIEEAVAKAEKMGVKV"
     gene            complement(3596..4483)
                     /gene="atpG"
                     /locus_tag="PKK81_10265"
     CDS             complement(3596..4483)
                     /gene="atpG"
                     /locus_tag="PKK81_10265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNV33307.1"
                     /translation="MPSLQSLRRKIAAFKNTQKITKAMKMVAAAKLKRSQDRILAARP
                     YAHKMRGVLSNLSQRVNRTSHPLLQKREGKKIEILVVTSDRGLCGGFNGNIVRKSSEF
                     VRQCEAQGLSVNLSLIGRKGRDYFRRRAWPIRQEWTGIFDKLSFEHAIDIGGDLTENF
                     VKGTFDELYVVYNEFKSAIQQRVIVEKLFPIDAAAEFGAAPSATEQSAVGGSYLYEPD
                     EGELLNVLVPKHFQIQAYRILLESAAAEHGARMAAMDGATRNAGQLIKKVTLYYNKTR
                     QAAITKELMDIVGGAEALK"
     gene            complement(4548..6065)
                     /gene="atpA"
                     /locus_tag="PKK81_10270"
     CDS             complement(4548..6065)
                     /gene="atpA"
                     /locus_tag="PKK81_10270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNV33308.1"
                     /translation="MQIKAEEISSIIKEKIKGFDQQVDVSQTGSVIQVGDGIAKIYGL
                     DGAMAGEMLEFPGGLYGIALNLEEDNVGAVLMGDDVGIKEGDPVKRTGRIAEIPVGEA
                     LIGRVVNAIGQPIDGKGPINSQHSSRIEVVAPGVNTRQSVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGLGVFCIYVAVGQKRSTVARVVKTLEENHAM
                     EYTMVVAATASDPAPMQFLAPFSGAAIGEYFRDNGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDKLGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLGSDLFYSGIRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREM
                     AAFSQFGSELDKATQMQLARGVRMVELLKQGQYKPMPVADQVLSIYAGVNGFLDDVPV
                     DKVLQFEADLLHYVAQNHQDMRKDIATVGKIDDKVGGKLKEIITTFKQKMGYGAK"
     gene            complement(6068..6607)
                     /gene="atpH"
                     /locus_tag="PKK81_10275"
     CDS             complement(6068..6607)
                     /gene="atpH"
                     /locus_tag="PKK81_10275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNV33309.1"
                     /translation="MIKTAVARRYAKALFELLDTPSIEPARSALNGLGEAFTQSAALR
                     HAIASPVFPEETKLGALIELATRLGCPPVGQRFLELLVKKNRVSFLPDIAEAFAKLVD
                     QSKGTQQVLVSSANALPAAEQDRITTRLRDLLQRDVDVTFHTEPEHVAGLHIRLGSTV
                     VDSTVRGRLSAMQRVLTKE"
BASE COUNT         1363 a         1973 c         1884 g         1469 t
ORIGIN      
        1 attgaagcag accgggatgt gtataccaga catgtcgtcg agcgcttgca aacaaagggt
       61 gtggtcgtgg catcggagaa ctgggagcag aagaatacgc tgcccctacc ggctcaattt
      121 ttgatggagt cgggacggta catcgccaag aaagggttag gaattcagta ccggctgatc
      181 agcttgtggc cgatcaacaa gcgcaatgtc gcggcgaacg agcttgagaa ggccgggctc
      241 ggaaccatct tgacgcaacc gaatcgaccc cacaccggct ttatgaagat cggggagacg
      301 cgctattttc aagcggtgta tgccgatctc gcggccaccc aagcctgcct cggttgccac
      361 aacgcgcatc ccgacagccc caaacgtgat ttcaagctga atgatgtgat gggtgcaatc
      421 gtgattacga tccccgtcgg gcaataatgc cgcggatgtt cggcactctg tcactcaggc
      481 gccacgacaa taccggcagg acggaccacg atcctgccgg tgggaacagg tccggtcctt
      541 cacctgaaac cttgtcgagt ctgtttcagg gaggagtacg gatcacggga gccggtgtgg
      601 tgcggatctc gcgtggcttc aatcgatcaa tctctgcgtc cttgtctgcg agttgctgct
      661 tcagtacttc gatctcttcc cgaagttgaa gaatgatctg ttctgaatcc acctggccgg
      721 agtggaatga tcccggccct ggtgcatcag cttctggtgc cgagactggc gcgtgcgatc
      781 cactgacagg aacgggagcc gccgctgatc ccctgtgctg gagagaggaa agaaatgcag
      841 catgatcgag gatcaattcg ctggcggacg ctcgatgccc acctgaccaa ttggcttttg
      901 tcccgatcac aaatcgaggt aagtcaaaga ccaaggcgcc gccgctgcct tgaacggaat
      961 ggagaggatt cgcccgcaca cgcgcaagct cttccgaatt ctccgcgagc gcgagacggt
     1021 gattggcgat caccagatgg agcttgccct cctcaacaaa catgccgccg gacgtggtct
     1081 cacgacccgt cccctcttgg ccaaccaaca taaagacgat ccattcacgg gatgttgctt
     1141 cttgaaagga cttacgcaca gtgggcataa ggaggttgat ctcttccaac gagaataccg
     1201 aacgcggcgg tctcgcgtta tccataagac cgactcgttc ctgaagcaac aatcggctga
     1261 ggatggcaaa gagttcttca tccctccaaa cactcggatg atcatatcgt ggggatgcac
     1321 tgccggcttc gagaagggag tcgagccgca caaaccagga ggagtcctgt tcaatgacac
     1381 gactcgttag cgtgggcgcg gtacaaccga gacagaggag gatcgtgagg ccaaggaagt
     1441 aagaattgaa tgacgtggtt cgtaacaccg gaggtccccc gacgacggat cagggaggtg
     1501 caatcggcag actacgtgaa gtatcgggaa gagaggttag gcgtgcccgg attttttggc
     1561 acggtccgcg atcttttttc ggagacgggc cttttcgagg ctgatctgag cttccttcac
     1621 ttcggaaggg agcccaccgg cctgcagtct ctgttcggcc ttttcgacgg cctgttgcgc
     1681 acgaccgaca tcgatgtctt cggccttctc ggcgatctcc gccatcacag tcactttgga
     1741 tggtgtgacc tcggcgtagc cccacaggat cgacatgtag tgccacgcgt cctgagtctt
     1801 gtaccgcagc tctccgatgc gcaggctgga gagaaaatgg cagtgtccag gcaggacgcc
     1861 gaattctccc tcgctacccg gagcgatgac ttcatcgacc tcctggctca ggagcaactt
     1921 ctccggcgtc acgacttcta atagaatctt cccggccatc ttctcttacg actcccttgt
     1981 cccgcatgag cgggaatcaa tgcgtcttct tctgcgcgcg ttcaacgagg tcttccaaaa
     2041 cttcgcgtgg cgacgagcac cgaggacgcg cggtttccgc gctcctaaac cttgactccc
     2101 atcttttccg ccttcgcgac ggcttcctcg atcggaccga ccatgtagaa cgcctgctcc
     2161 ggcaggtggt cgtacttccc ttcgaggatt tccttgaagc tccggaccgt atccttgagc
     2221 ttcacgtact tgcccggcgc gccggtgaac gcctcggcca catggaacgg ctgggaaagg
     2281 aagcgctgaa ttttgcgcgc gcgcgccacg gccatcttgt cgtcttcaga caattcgtcc
     2341 ataccgagaa tggcaatgat gtcctgcaaa tctttatacc gctgcaacac ggactgaacg
     2401 ccgcgggcca ccttgtaatg ctcttctccg atgacttgcg gatcgagaat acgtgacgtg
     2461 gaatcgagtg gatcgaccgc cgggtaaata cccaactcag ccaactgccg ggacaacacg
     2521 gtcgtcgcat ccaagtgcgc aaaggccgtc gccggagccg ggtcggtcaa gtcgtcggcc
     2581 ggcacgtaaa tcgcctgcac tgaagtgatg gagcccttct tggtagacgt aatccgttcc
     2641 tgtagcgcac ccatttcggt ggacaggttc ggctgataac cgaccgccga tggcatacgg
     2701 ccaagcaacg cggacacttc cgatcccgcc tgcgtgaacc ggaagatatt gtcgacgaac
     2761 aacaacacgt cctgattttc ttcgtcgcgg aaaaattcgg caacagcgag tccagtcagc
     2821 gcgacccgta gacgggctcc gggtggctcg ttcatctgac cgtagaccaa tgccgccttg
     2881 gacttcgtat ggtcgtcggg atcgatgacc ttggactcct gcatttcgtg ccaaaggtcg
     2941 ttgccctcac gggtacgttc accgacgccg gcgaacaccg agaatccacc gtggtggagc
     3001 gcgatgttat tgatgagctc catgatgatg acggtcttgc cgactccggc tccgccgaag
     3061 agcccgacct tgccaccctt gctgtacggc tccaacagat cgaccacctt gataccggtc
     3121 tcgagcactt cggtcttcgt gtcctgatct tccaatcgcg gcgcaggccg gtgaatgggg
     3181 taggttttct tcgccttgat cggacccttt tcatcgaccg gctcacccaa cacgttgatc
     3241 agccgtccca gggtctcacg accgacggga acagaaatcg gggcgcccgt atcctgcaca
     3301 tccatgccgc gagtcagacc gtccgtcgtc gacatcgcga tgccgcggac gcggttctca
     3361 ccgagatgcg atgcgacttc aagcgtgatc cgcacggccg gcttcccggc tgccttgttc
     3421 tcttcctgcg tcaccttgag cgcattgtag atgttcggca attgaccggg gggaaattcc
     3481 acgtcgacca cggggccgat gacttgaatg acttttcctg tgctcataag gctcctcttg
     3541 tcgtcaatga tgcacccggc acattaccgt gatgcagcat tcctcattcc acattttatt
     3601 tcagggcttc tgcgccgccc acgatgtcca tgagttcttt ggtgatagcc gcctggcgag
     3661 tcttgttgta atacaaggtg acttttttga tgagttgccc tgcattgcga gtcgcaccgt
     3721 ccatggctgc catgcgtgcc ccgtgttcgg ccgccgccga ctccagaagg atgcgatagg
     3781 cttgaatctg aaaatgcttc gggaccaaga cattcagaag ctcgccctca tccggctcat
     3841 acaagtaact accgccgact gcggactgct cggtcgcact cggcgccgct ccgaattcgg
     3901 cggccgcatc gatggggaac aatttctcaa cgatcacccg ctgctggatc gcagacttga
     3961 actcgttgta gacgacgtag agttcgtcga acgtgccctt cacaaaattt tccgtcaggt
     4021 cgccgccgat gtcgatggca tgttcgaagc tcagcttgtc gaagattccg gtccactcct
     4081 gtcggatcgg ccaggcacga cggcggaaat agtcacgccc cttacggccg atcaggctga
     4141 gattgaccga gagcccctgc gcttcgcact gtcggacgaa ctctgagctc ttccgcacga
     4201 tgttgccgtt aaatccgccg cacaagccgc ggtcgctcgt gacaacgaga atttcgattt
     4261 tcttcccctc gcgtttttga agcagcgggt gggaggtacg gttgacgcgc tgactgagat
     4321 tgctgagcac cccgcgcatt ttatgggcgt aaggcctcgc agccaaaatg cgatcctgcg
     4381 aacgcttgag cttcgcggcc gcgaccattt tcatggcctt ggtaatcttc tgcgtattct
     4441 tgaaggcggc gattttccgc cggagagatt gtaagctcgg cattcttgta cgctctcagc
     4501 agtcagcgct cagacctcag ctccatgctg aaggcgaagg ctgattgcta ttttgctccg
     4561 tagcccattt tctgcttaaa ggtcgtgatg atttctttca gcttgccgcc gaccttgtca
     4621 tcgatctttc cgaccgtggc gatatctttc cgcatgtcct ggtgattctg cgccacgtaa
     4681 tggagcaggt ccgcctcgaa ttgcagcacc ttgtctacgg ggacatcgtc caggaatccg
     4741 ttgacgcccg cgtaaatcga caagacttga tcggcaaccg gcatcggctt gtattgccct
     4801 tgtttgagca attccaccat acgtacgcca cgggcgagct gcatctgggt cgccttgtcg
     4861 agttcgctcc cgaactggga gaaggcggcc atttcacgat attgcgcgag atctaaccgg
     4921 agcgtacctg cgacctgctt catagttttg atctgcgcgg atccgccgac acgtgacacc
     4981 gacaatccga cgttgatcgc gggacgaata ccggaataga acaagtcgct gcccaggtaa
     5041 atctgtccat cggtaataga aatgacgttg gtcggaatgt aggccgacac gtcgccggct
     5101 tgcgtctcga tgatcggaag ggccgtcagg ctgcctccgc ccaacttatc gctcaacttc
     5161 gccgcgcgct ccagcaagcg ggagtgcaga tagaacacgt cgcccggata ggcttcacgg
     5221 cccggcggac ggcgcagcag caaggacagc tgacgatagg ccaccgcgtg cttggacaaa
     5281 tcgtcgtaca caatcaacgc atgcttgccg ttgtcgcgga aatattcacc gatcgcggcg
     5341 ccggagaagg gggccaggaa ctgcatcggc gccggatcgc tggccgtcgc cgccacgacc
     5401 atggtgtatt ccatggcgtg attttcttcg agggtcttga ccacccgcgc aaccgtagaa
     5461 cgcttctgac cgacggcgac gtagatacag aatacaccga ggcccttctg gttgatgatg
     5521 gtatcgacgg cgatagctgt ttttccggtc tgccggtctc cgatgatcaa ctcacgctga
     5581 ccacgaccga tggggatcat ggcgtcgatg gccttgatgc ccgtttgcag cggctcacgc
     5641 accgactgtc tcgtgttcac accgggtgcc acgacttcga tacgggaaga atgttgggaa
     5701 ttgatcgggc ctttgccgtc gatcggctga ccgatcgcgt tcaccacccg tccgatcaac
     5761 gcttcaccaa ccgggatttc cgcaatacgc ccagtgcgct tgaccggatc gccttccttg
     5821 atcccgacat catcacccat caacacggcg ccgacattgt cttcttcgag gttcaacgcg
     5881 atgccgtaca ggccccccgg gaactcgagc atttctccgg ccatcgcgcc gtctaatccg
     5941 tagattttcg cgatgccgtc tccgacctgg atgacggaac cggtctggct gacatcgacc
     6001 tgttgatcga agcccttgat cttctcttta atgatggagc tgatctcttc tgccttgatc
     6061 tgcatggcta ctccttggtc aacacacgct gcatggcgct cagtcggccc cggaccgtgc
     6121 tgtctacgac ggtgctgccc aggcggatgt ggaggccggc gacatgttca ggttctgtat
     6181 ggaaggtgac atcgacgtcg cgctgtaaaa gatcgcgcaa tcgggtggtg atccgatcct
     6241 gctcggccgc gggcaaggca ttcgcggatg acacgagcac ttgctgcgtc cccttggatt
     6301 gatccacgag cttggcgaag gcctcggcga tgtccggcag gaaactgacc cgattctttt
     6361 tgaccaagag ttcgagaaat ctctggccga ccggcgggca tccgagtcga gtggccaatt
     6421 caatcagcgc acccaatttg gtttcttccg ggaacacagg agaagcgatg gcatggcgta
     6481 acgcagcgga ctgcgtgaag gcctctccca gcccgttcaa cgccgagcgg gcgggctcga
     6541 tgcttggggt atcaagaagt tcgaacagag cttttgcgta tcgacgtgcg acggctgtct
     6601 taatcacaat ggactatccg ctacggttaa ccgtgttttg atcaaacggg aagcttgcgt
     6661 gcggtgggca cgacaaaaag cgcgccaag
//
LOCUS       DAOQRM010000136         6504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_11803_length_6504_cov_12.351682, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000136 DAOQRM010000000
VERSION     DAOQRM010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11803_length_6504_cov_12.351682"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1148
                     /locus_tag="PKK81_10280"
     CDS             57..1148
                     /locus_tag="PKK81_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNV33310.1"
                     /translation="MPQVLVLGAGKIGSLVAGLLSASGQYHVHLADLTLDAPKRLVDD
                     LSLSAVTPCALDVRRPDAVAAYVSSSPFDAVISSLPYFCNPTVAEIARAHQLHYFDLT
                     EDVAVTGRVKAISAGADHAFIPQCGLAPGFISIVTNDLIGHFESIDNVKMRVGALPVH
                     PSNALKYSLTWSTDGLINEYANVCVGIEGGEEVQLQPLEGYETIELDGLLYEAFNTSG
                     GLGTLADTYRGRVRTMNYKTLRYPGHCEKIQFLMKDLKLNEDRETLKRILERAIPQTQ
                     QDVVLIYAAVTGTRQGELFEETYVKKVYPRTILGRLWSAIQVTTASSLCCVVDLVMAR
                     PSAYRGFVTQEHFHLQDVLNNRFGDCFRA"
     gene            1156..2712
                     /locus_tag="PKK81_10285"
     CDS             1156..2712
                     /locus_tag="PKK81_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNV33311.1"
                     /translation="MTIQELFKTLGLTQEQPGGCLSSSTWSRTTDAGIVESRNPSTGE
                     PLAQVYGCSLADYDALVAGSQQAQASWRMVPAPKRGEVVRLIGQALRVHKDALGTLVS
                     LEVGKIKAEGDGEVQEMIDMADFAVGLSRMLYGQTMHSERAQHRMYEQWHPLGVVGVI
                     TAFNFPVAVWAWNAFIAAVTGSTVLWKPSPKAPLCALAVQRLCNRVLEEAGYPGVFAL
                     FTTDRVELAERMVRDERLPLISFTGSVPVGRHVAEVVGHRLGRSLLELSGNNAVIVDE
                     TADLDLATRAIVFGAVGTAGQRCTSTRRLIVHESQYELLVPRLLSAYAQVKIGDPLEP
                     DMLMGPLIDGEAVTRYLSALEEVKKAGGEVLCGGCVLSRPGYFVEPTIVRAQNHWDIV
                     QRETFAPILYVMTYRTLDEAIRMHNAVPQGLSSALFTTHLRNSETFLSAIGSDCGIAN
                     VNIGTSGAEIGGAFGGEKTTGGGREAGSDAWKAYMRRQTSTINWGTELPLAQGVTFGR
                     AEGGNDGARS"
     gene            2693..3295
                     /locus_tag="PKK81_10290"
     CDS             2693..3295
                     /locus_tag="PKK81_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33312.1"
                     /translation="MMGQEADLDAVMARMTADFLAQNSAGRMLRRALDDVGVGFIPVI
                     DHVTIRTMDIDRRAEEFLALGYRYDETLHYDDWYAKVFRWAGYPALFVDQAYPDDRGK
                     TSIIPRWVEKFGDQVFHHVAVRVEDIEQAILKLKAKGVVFAGEIVGARGGTLRQIFTA
                     PEMLDGQPFSVLELAERHQGYQGFLPPQADSLMRSTMRGL"
     gene            3466..4536
                     /locus_tag="PKK81_10295"
     CDS             3466..4536
                     /locus_tag="PKK81_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNV33313.1"
                     /translation="MVQSNATLRLILALLVLCALGAGSGSVQASPSGHAPHGTVTIDG
                     VTVPDVGPLPTVVPIPSSNLNYTAKIELGKQLYFDGRLSKNNAISCAFCHNPGTGFAD
                     PRQTSIGVGGGVGGRQSPTVYNTGLNHVQFWDGRARSLEEQAIGPIHNPVEMAETHEH
                     VVAKLGKIKGYQQQFRAVFGTDVNLQGIAEAIAAYERTVLSTNSAFDKYVLGAQKAMD
                     EAAVRGLALFKGKARCILCHNGPNFTDNQFHNLGVPQVGPMKEDLGRFAVSRAEKDRG
                     AFKTPTLRSITETAPYMHDGAFKTLEEVVEFMDQGGGSNPNLSPLVKPLNLTAEEKSD
                     LVAFLKALAGEPIPFSMPQLPK"
     gene            4650..5156
                     /locus_tag="PKK81_10300"
     CDS             4650..5156
                     /locus_tag="PKK81_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HNV33314.1"
                     /translation="MFRNLCVLGASTIALALGGPALAADMKPGPADGFDIHVMAPHKM
                     EDGSVAGPFHHYCKAVKPEVLQCLLFESTDPNAVLTDVEYFVAKPVSRSAVPLDVWNK
                     FYHDHEVEIATGRVQVLDMPDAQAKEVAAAAAKTDGIIFHLWPKGAKAPDGSVEHPTS
                     VGHKPRTE"
     gene            5209..5763
                     /locus_tag="PKK81_10305"
     CDS             5209..5763
                     /locus_tag="PKK81_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HNV33315.1"
                     /translation="MRSAILMFGLCAAALTAAGCAETAREMKSSSAAPAAATAAKAMP
                     TPAQGYTIHVMAPHKFEDGSVHGPYHHYCKPISPEVLQCLLFESTDANALLTDIEYFV
                     AKSVSRAHVPLETWNKYYHDHEVEIATGRVQILDMPEAQAKEVAAIAAKTDGIIFHLW
                     PDGAKAPNGQVGHPQSVGHKHRTE"
     gene            5845..6291
                     /locus_tag="PKK81_10310"
     CDS             5845..6291
                     /locus_tag="PKK81_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV33316.1"
                     /translation="MDAGRLCLMGWLLLGGVSVGWAANSEVLRPRVPIDQIEAARTVT
                     NPFPVTPEMLQQGKALFEGKAFCRACHGSDGKGLGMDLDYSTFKGPLPRNFTDRLWQQ
                     ARTDGELFWILKNGSPGTDMAPFIPLVLTEEEAWQVLMYVRSFGGR"
BASE COUNT         1298 a         1727 c         2052 g         1427 t
ORIGIN      
        1 ttcagaaaag gcggttcttt tcgtccgaag gagtataatg ggctgtgagg tgactgatgc
       61 ctcaggtgct ggtgcttgga gccggaaaaa tcgggtccct cgtggctggg ttgttgtcgg
      121 ctagtgggca gtaccacgtt cacctagccg atcttaccct tgatgccccg aagcgactgg
      181 ttgatgactt gtcgttgtcc gcggttactc cctgtgcgtt agacgtgcgg cgaccagatg
      241 cggttgcggc atacgtcagt tcctccccct tcgatgcggt gatctccagc ctgccgtact
      301 tctgcaaccc gaccgttgcc gagatcgcgc gagcccatca actccattat ttcgacctga
      361 ccgaagacgt ggccgtgacc ggtcgcgtca aggcgatcag cgcgggtgcc gatcatgcgt
      421 ttattcctca gtgcgggtta gcgccgggat ttatcagtat cgtgacgaat gaccttatcg
      481 gccattttga atccatcgac aacgtgaaga tgcgtgtcgg cgccctgcct gttcatccga
      541 gtaatgcgct gaagtactcc ctcacctggt ccaccgacgg gttgatcaat gagtatgcca
      601 acgtttgtgt cggcattgag gggggggagg aggtccagct tcagccgttg gaaggctatg
      661 aaacaattga attggacggg ctgctctacg aggccttcaa cacgtccggc ggcctgggca
      721 ctctcgcgga cacgtaccgc ggacgggtcc gaaccatgaa ttataagacc ctgcggtatc
      781 ccgggcattg cgaaaaaatt caatttctca tgaaggacct caagctgaat gaggatcggg
      841 agacgctcaa gcgaatcctg gagagagcga ttcctcagac gcagcaggac gtagtgttga
      901 tctatgcggc cgtgaccggc accaggcagg gtgagttgtt cgaggagacc tatgtgaaaa
      961 aggtgtatcc gcggacgatc ctcgggcggc tctggtctgc gattcaggtc acgacagcgt
     1021 cgtcgctttg ttgcgtcgtt gatcttgtga tggccagacc ttcagcttat cgtggctttg
     1081 tcacgcagga acactttcac ttgcaggatg tgctgaacaa tcgcttcggg gattgtttcc
     1141 gggcctgacg gcatcatgac gatacaggaa ctgttcaaga ccttggggtt gacgcaggaa
     1201 cagcccgggg gatgtctctc ctcttcgacg tggagtcgca ccaccgacgc cggtatcgtc
     1261 gagtcccgca atccatcaac tggagaaccg ctggcgcaag tgtacgggtg ttcccttgcc
     1321 gattacgatg ccctcgtagc cggatcgcag caggcccagg cctcgtggcg tatggtgccg
     1381 gcgccgaagc gcggcgaggt ggtccgtctg attggtcagg cgctacgcgt gcacaaggat
     1441 gccttgggta cgctggtgtc gttggaggtg ggcaagatca aggccgaggg cgatggcgaa
     1501 gtgcaggaga tgatcgatat ggcggacttc gccgtcgggc tgtcacgcat gttgtacggg
     1561 cagaccatgc attcggagcg tgcacagcat cggatgtatg agcaatggca tccattgggt
     1621 gtggtgggtg tcattacggc gtttaacttt cctgtggcgg tctgggcctg gaatgctttt
     1681 attgccgcgg taaccggcag caccgtgttg tggaagccgt caccgaaagc tccactttgt
     1741 gcattggcgg ttcagcggct gtgcaatcgt gtgctggaag aggccgggta tcccggtgtt
     1801 ttcgcgctct ttacgacgga tcgcgtcgag ctggccgagc ggatggtgcg ggacgagcgg
     1861 cttccgttga tctccttcac cggctcggtg cccgtcggga ggcacgttgc ggaggtggtc
     1921 ggacatcggc tgggccggag tcttctggaa ttgagcggga acaacgccgt catcgtggac
     1981 gaaacagccg atctggattt agcgacgcgc gccatcgtct tcggcgcggt cggtacggcg
     2041 ggacagcgct gcaccagtac caggcgactg attgtgcatg aatctcagta cgagctgctg
     2101 gtcccgcgac tgctgtcggc ctatgcgcag gtgaaaatcg gtgatcccct cgagccggat
     2161 atgctgatgg ggccgcttat cgacggggag gcggtgacgc gttacctgtc tgcgcttgag
     2221 gaagtgaaga aggcgggcgg tgaggtgcta tgcggaggat gtgtcttgtc tcgccccgga
     2281 tattttgttg agcccacgat cgtgcgggcg cagaaccact gggatatcgt ccagcgggaa
     2341 acctttgcgc cgattctcta tgtcatgacc tatcgaacgc tggacgaggc catccggatg
     2401 cacaatgcag ttccacaggg actgtcctcc gcgctgttta ccacccatct tcggaacagt
     2461 gagaccttct tgtccgcgat cggcagcgac tgtggcattg ccaatgtgaa catcgggacg
     2521 tccggtgcgg aaatcggcgg ggcattcggt ggagaaaaaa ccacgggtgg cggacgggaa
     2581 gccggttccg atgcgtggaa ggcctacatg cgtcgacaaa ccagcacgat taattgggga
     2641 actgagttgc cgttggccca gggcgtgacg tttggacggg cggaaggagg gaatgatggg
     2701 gcaagaagct gacctcgatg cggtgatggc tcgtatgacg gccgactttc tggcgcagaa
     2761 tagcgcagga cggatgcttc gccgggcgct ggacgacgtc ggggttgggt ttatcccggt
     2821 gatcgaccat gtcaccatcc ggacgatgga tattgatcgg cgcgccgagg aatttctcgc
     2881 actgggttat cggtacgatg aaacgcttca ttatgacgat tggtatgcca aagtattccg
     2941 gtgggccggg tatccggcgc tgtttgtcga tcaggcctat ccggacgacc gtggcaagac
     3001 cagcatcatc ccacgctggg tcgagaaatt cggtgatcag gtgtttcacc atgtggcggt
     3061 ccgtgtcgaa gacatcgagc aggccattct caaattgaag gcgaagggcg tggtctttgc
     3121 cggcgagatc gtcggtgccc ggggaggcac gttgcgccaa atttttacag cccctgagat
     3181 gctcgatggg caaccctttt ccgtccttga attggccgag cggcaccagg gttatcaggg
     3241 atttcttcct ccacaagctg acagtctcat gcgctcgaca atgcgaggcc tgtaaggccg
     3301 taggcttcgg ccgaccgctg ctctgctgtc gcgatgtgtt ggcagcctct tcccgccgcc
     3361 caaagagtgc ctcgaccaga tgggttgaat tgcgtacaat gcgccgtgtc gaccaaccag
     3421 gtcgagagtg tctggcatgc gcgatctcaa caggaggttc tcttcatggt gcaatctaac
     3481 gcgacgttac gcttgatcct cgcactgctg gtgctctgtg cacttggagc aggaagtggt
     3541 tccgtgcaag catcgccgtc cggccatgcc cctcatggca ccgtgacgat cgatggtgtc
     3601 acggtcccgg atgtcgggcc cctgccgacg gtggtcccga taccgtcctc caatctcaac
     3661 tacaccgcta agatcgaact cggcaagcaa ctctattttg acggacgact ctccaaaaat
     3721 aacgcgatct cctgcgcctt ctgccacaac cccgggaccg gatttgcgga tccccgccag
     3781 acgtccatcg gagtcggcgg aggcgtcgga ggacgacagt cacctacggt ctacaatacg
     3841 ggattgaatc acgttcagtt ttgggatggt cgggcacgtt cgcttgaaga gcaggccatc
     3901 gggccgattc ataaccccgt ggagatggcc gagacgcatg aacatgtggt ggccaagctg
     3961 gggaagatca aagggtatca gcaacaattc agggctgtat ttggaacaga tgtcaatctt
     4021 cagggcattg ccgaggcgat cgccgcctat gagcgcacag tgctctccac caattccgct
     4081 tttgataaat acgtgttagg cgcgcagaaa gccatggatg aggcggcggt tcgaggactg
     4141 gcccttttta agggcaaggc tcgttgcatc ctctgccaca atgggccgaa cttcaccgat
     4201 aatcagtttc acaacctcgg cgtgcctcag gtcggtccga tgaaggaaga ccttgggcgt
     4261 tttgctgtca gcagggcgga gaaagaccgg ggcgcattta aaacgccgac gctccgtagc
     4321 atcacagaaa ccgctccgta tatgcacgat ggcgcgttta aaacgctgga agaggtcgtc
     4381 gaatttatgg atcagggcgg cgggagcaat cccaatctga gccccctcgt caagccgttg
     4441 aatctgacgg cggaggagaa aagcgatctc gttgcattct tgaaggcgct ggcaggagag
     4501 ccgatcccgt tcagtatgcc acaattgccg aaataaaaca cgaatccttt tgggccgcct
     4561 gtgactctca cgggcagata cgtgtgcctt ggtcagacta ttttgtccaa gcatcggtac
     4621 gttccaaccc caaccatagg aggccatcta tgtttcgaaa tctttgtgtg ctcggtgcga
     4681 gcaccattgc gctcgccctc ggcgggcctg cacttgcggc ggatatgaag ccgggtccgg
     4741 cggacggatt cgacattcac gtcatggcgc cccataaaat ggaagatggg tcggtggcag
     4801 gcccgttcca tcactattgt aaggccgtca agccggaagt cttgcaatgt ctgctgttcg
     4861 aatccacgga tcccaatgcc gtgctcaccg acgtggagta ttttgtggcc aagcctgtgt
     4921 cacggtcggc ggtgcctctc gatgtctgga acaagttcta tcacgaccat gaggtcgaaa
     4981 tagcgaccgg gcgtgttcag gtgctggata tgcctgatgc tcaggccaaa gaagtggcgg
     5041 cggctgcggc caagacggat ggcattattt tccacttgtg gccgaagggt gcgaaggctc
     5101 cggatggatc ggtggagcat ccgacgtccg tggggcataa accgcggacg gagtaagtct
     5161 gcgaatagcg cgtgatccat cctgtgccct aattgtaagg aggccactat gcgaagtgcg
     5221 attttgatgt ttggattgtg tgcggcggcg ttgacggcag ccggttgtgc ggagactgcc
     5281 cgcgaaatga aatcctcctc tgcggcacca gctgccgcca cggcggcgaa agctatgccg
     5341 acaccggcgc agggctatac cattcatgtc atggctcccc acaaatttga agacggatcg
     5401 gtgcatggtc cgtatcatca ctactgcaag ccgatttccc cggaagtgct gcagtgtttg
     5461 ttgttcgagt cgacggacgc caacgccttg ctgacggaca ttgaatattt cgtcgcaaag
     5521 tccgtctctc gtgcgcatgt tccgttggag acgtggaata agtattacca cgaccacgaa
     5581 gtagagattg ccacagggcg ggtgcagatt ctggacatgc cggaggcgca ggcgaaggaa
     5641 gtcgctgcca ttgcggccaa aaccgacggg atcatttttc atctttggcc ggacggagcc
     5701 aaggcgccga acggtcaggt ggggcatccc cagtctgtag gccacaagca tcgcactgaa
     5761 tagccggagc gacgcagatc catcgggcgg agggggcaca gccctctccg cctgtgctat
     5821 ttctgatgaa agggagacgc tgtgatggat gctggacgtc tatgcctgat gggatggctg
     5881 ctgctcggcg gtgtatctgt tggttgggcg gcgaattccg aagtgttaag gccccgtgtt
     5941 ccgatcgacc agatcgaggc ggccaggact gtaaccaatc cctttcctgt tacaccggaa
     6001 atgctccagc agggaaaggc gctcttcgaa ggcaaagcat tctgtcgcgc ctgccacggg
     6061 tcggatggga agggactagg aatggatttg gactattcca ccttcaaagg accactccca
     6121 cggaacttta ctgataggct atggcagcag gccaggacgg acggtgagct gttctggatt
     6181 ctgaagaacg ggagtcccgg gacggatatg gccccgttta ttccactcgt cctgaccgag
     6241 gaggaggcct ggcaagttct catgtatgtc cgttctttcg gtgggcgatg acgcgtgggc
     6301 ctcgtgccgc actcacggtt tcaatgtcgt cggtaggctc ggttccttgc gggcggcttg
     6361 gttgtcggga tgccgcaagc tcgccgcgct gagacaggtg aatggtcggc attgaccgaa
     6421 gctgaggcgc ggtataccga caatgtgtct gactggtccg ttacgcggcg cctgcactat
     6481 caaaggatct cagccagccg acga
//
LOCUS       DAOQRM010000137         6502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_11811_length_6502_cov_16.761284, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000137 DAOQRM010000000
VERSION     DAOQRM010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11811_length_6502_cov_16.761284"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKK81_10315"
     CDS             <1..265
                     /locus_tag="PKK81_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNV33317.1"
                     /translation="HLPAGAGQPESAGAPQGSIQSMTDFGQPGFGGPCPPPGDKPHRY
                     IFTVFALKVDQLPLKKEASGAMVGYYLNQNAIGKASFTGTYGR"
     gene            302..757
                     /locus_tag="PKK81_10320"
     CDS             302..757
                     /locus_tag="PKK81_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33318.1"
                     /translation="MSLDAELGKTMLQLITSRYEDRHWRKKIEKTLSLPQSGVGDDHQ
                     QQVFMYLKLNLKAYKSRRADPDSWILGGYATKEVIDRAKFQPHLVGEGISADDVAFLG
                     VDPGPEIDEAWWEEMLVAWFGAPEEEESSEEATADEAATPDAVKDPART"
     gene            complement(821..1492)
                     /locus_tag="PKK81_10325"
     CDS             complement(821..1492)
                     /locus_tag="PKK81_10325"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNV33319.1"
                     /translation="MSSPLTTLQELAESLHNCQRCPLAKLGRRQVVFGVGNPHASVMF
                     VGEAPGFHEDQKGEPFVGAAGQLLNDLLESAGLSRADIYIANVIKCRPPNNRDPEPQE
                     VETCKPFLLQQIAMIRPKLVCSLGNWATQTLLERKVGITRVRGQAFYLKEFVLFPLLH
                     PAAALHQGTMLQPLREDFQKLREFLDRHSQPPATQEGAASSTTETVRIDPPATIAQQM
                     DLFGS"
     gene            complement(1583..2893)
                     /locus_tag="PKK81_10330"
     CDS             complement(1583..2893)
                     /locus_tag="PKK81_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV33320.1"
                     /translation="MNRTVLILGGGIAGLTAALHLAADGHAVTVIEQADHLGGRLCHA
                     PPPLLLEAHTATWSLLKTLGKDTATRRLRHTPLEFLQATGARIQFLHLPLPSPLNTLL
                     GTTLFQGLSMRDRWHLLSFLERTWEQDPPLPNDLDTRAADEWLASIGQSEHARHGVWN
                     SLARLLLGAALPQVSAELFMRTLRRCFLTGARATKLIIPPHGLDSFLLTPLRAQLDRM
                     GVRSRLNTTATQLHFTQDRVAHVELADRTRLTADWYIAALPHHRLTPLLPERVVTHYA
                     YFQQISRLSESPLVIVRLHLAQPVEQTQLVLLERNRFHWMIRHADEERHEQAAVLWAV
                     AVDEPDLLPQSKDDLVQLTLKDMERAFPGGPLPRLIESDVVRLPSAILTTKPGTHQCR
                     PLSHSPFTNFLVAGAWTDTGWPASLESAILSGQRSAALVPADAS"
     gene            complement(2890..3765)
                     /gene="hpnD"
                     /locus_tag="PKK81_10335"
     CDS             complement(2890..3765)
                     /gene="hpnD"
                     /locus_tag="PKK81_10335"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNV33321.1"
                     /translation="MMTPAEAQTYCTTLTKKSGSNFYYSFLFLPKARREAMYTVYAFC
                     KEVDNAVDEPPPGSHPQEELARWRRELAAAYDGTPTFPVTVSLARHVRELSIPQAYFE
                     ELIKGVEMDLTTTRYATFDQLSLYCYRVASVVGLICLHVFGTTSPRAQDYAVNLGMAF
                     QLTNILRDLGNDAECGRVYVPQEDLARFAYREEDLLHRRYKPEFTEFMRFEVGRAKEF
                     YAKAARALDSLPRAERRALTVAEIMRGVYSRILHRIEQSGYRVLGDRVTLTPSHRLAV
                     AAGVWLRSRLPSSTP"
     gene            complement(3773..4705)
                     /locus_tag="PKK81_10340"
     CDS             complement(3773..4705)
                     /locus_tag="PKK81_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNV33322.1"
                     /translation="MKICSLVPGATEVVAALGCQHDLIGISHECDFPPSLAQIPVMVR
                     PRIESHHLSSAEIDQQVGALLTAEAGLYELDETRFLAARPDLIIAQALCDVCAITPSQ
                     LERVVHALSPPPRLLTLSPGRLDDILLDIETIGTAIGREEAGTQLSAVLRSRLDTVRA
                     TVGAQATRPRVACIEWLSPLYTAGHWVPDMVAAAGGIDVLAQAGTASRKMDWDTLTAS
                     EPEVIVLMPCGFTVPRTKAELASMTAHPQWQHLPAVQRGEVYLVDALSYFSRPGPRLI
                     DGVEQLAAILHPASYGRHLPASVERLEGQTTHSH"
     gene            complement(4702..5409)
                     /locus_tag="PKK81_10345"
     CDS             complement(4702..5409)
                     /locus_tag="PKK81_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNV33323.1"
                     /translation="MSELRAIIFDFDGVIADTEPLHFAALRQVLAGIEISLTEAEYYT
                     DYLGFDDRGCFLAALQSHRRQASPTLLGELMEHKAHAYLAAVKQHLAIFPGVRELVHE
                     AAARYPLAIASGALRNEIELILEEAGLRKAFLHITSAEDVTRGKPAPDPFLHAMAGLN
                     SQPNQPALFPNDCLVIEDSLPGIRAARAAGMKVLAVANTHTVQDLGEADAITHSLADT
                     RLQDLQARLWGAAQGRP"
     gene            complement(5568..6311)
                     /locus_tag="PKK81_10350"
     CDS             complement(5568..6311)
                     /locus_tag="PKK81_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33324.1"
                     /translation="MVSSTGIPAMRSEWTNWHLLFASQPEPDLEFTEEDLDESMPPPA
                     PPMNSPKRSGKKPLLWILLLLLVGGVGYVAMDPDGAMQLVEPYLGGGAEPAQPVIQKP
                     PVKAQAPKQAPTVAPPVVADNSTPAPADVPAPSSAMSAPAIPAPAMPALQPAASAPKP
                     AAPTVRVAGPLYAEGQRVTVIADPTRPKSPIPLFVDAVGTKTSTTVLAGATLTILDGD
                     YQKRGWVYSVRAQDGRKGWVPERSLRLKR"
BASE COUNT         1249 a         1829 c         2185 g         1239 t
ORIGIN      
        1 gcacttgccg gccggagcgg ggcagccgga gagtgccggc gcgcctcaag ggagcattca
       61 gagtatgacg gattttggcc agcctggttt tggcgggccc tgtccaccgc cgggagataa
      121 accgcaccgg tatatcttca cggtgttcgc gctgaaggtg gatcagttgc cgctcaagaa
      181 agaggcctcc ggcgcgatgg tcgggtacta tctcaatcag aacgccatcg gaaaagcatc
      241 cttcaccggc acctacggcc ggtaagcgaa caatcatcag aacagagtaa gggaacagac
      301 catgtctctt gatgccgaac tgggaaagac gatgcttcag ttgatcacct cgcgatacga
      361 ggatcggcac tggagaaaaa aaatcgaaaa gacactcagt ctgccgcaga gcggggtcgg
      421 agacgatcat cagcagcagg tcttcatgta cctgaagctc aacctcaagg catacaagtc
      481 caggcgggcg gatccggatt cctggatctt aggcgggtac gccacgaagg aagtgattga
      541 tcgcgcgaag tttcagccgc acctggtcgg agaaggtatt tcggccgacg atgtggcgtt
      601 tctcggtgtc gatcccggcc ctgaaatcga tgaggcctgg tgggaagaaa tgttggttgc
      661 ctggttcggc gctccggaag aagaggagtc gtcggaggaa gccacggctg atgaggccgc
      721 aactcccgat gccgtgaagg atcccgcccg cacctgaagg cggctgtgac ggccccggac
      781 gggcggtcgt tcctgattca gtctcttcgg agcggtcttg ctatgacccg aacaaatcca
      841 tttgttgcgc gatcgtagcg gggggatcga tccgcaccgt ttcggtggtg ctcgaagcgg
      901 ctccctcctg cgtcgcagga ggctgactgt gccggtcgag aaactccctc agcttctgga
      961 agtcctcccg caacggctgc agcatggtcc cctggtgcag cgctgcggcg ggatggagca
     1021 ggggaaacag cacgaattcc ttcagataaa acgcctgccc gcgtactctc gtaatcccga
     1081 ccttgcgctc cagaagtgtc tgcgtcgccc aattgcccag cgaacagacc agcttcggcc
     1141 gaatcatggc gatctgctgc aagagaaacg gtttgcaggt ctcgacctcc tgaggttcgg
     1201 ggtcgcggtt attcggcggg cggcatttga tcacattggc gatgtagata tccgcgcgcg
     1261 acagccccgc tgactccaac aagtcgttga gcaattgtcc ggctgccccg acgaatggct
     1321 cccccttctg atcctcgtga aatcccggtg cctcccctac gaacatgacg gatgcatgag
     1381 ggttgccgac gccgaagacg acctggcgtc gaccgagctt ggcgagcggg cagcgttggc
     1441 aattgtgaag cgactcggcg agttcctgca gggtggtcag gggtgaagac atatgcgttc
     1501 cattcgagtg gagggccaaa tcttagtcgc ggtgggaggg gttgtcaacg tgatgtcgtg
     1561 agccattcgc ccatcggacg agtcatgatg cgtcggcggg aacgagcgca gcggagcgtt
     1621 ggccgctgag aattgcggac tccagactgg ccggccagcc ggtgtccgtc caggcgccgg
     1681 cgacgagaaa attggtaaac ggactgtgtg agagcggtcg gcactggtgc gtacccggtt
     1741 tggtggtcag gatggccgac gggagacgaa cgacatccga ctcgatgagc ctgggaagcg
     1801 ggccgccggg gaacgctctc tccatgtctt tcagggtcag ttgaacgagg tcgtctttcg
     1861 attgcggcaa caggtccggc tcatcgactg cgacggccca caacacagcg gcctgctcgt
     1921 gacgttcctc gtctgcgtgc cggatcatcc aatgaaaccg gttgcgttcc agaaggacga
     1981 gttgtgtctg ttcgaccggt tgcgcaaggt gcaggcgaac gatcaccagg ggagactcgc
     2041 tcaggcggct gatttgctgg aaataggcat agtgcgtcac gactcgttcc ggcaagagtg
     2101 gcgtgagtcg atggtgcggc agcgcggcga tataccaatc ggcggtgagc cgggtgcggt
     2161 ctgccagttc gacatgggcg acacgatcct gggtgaagtg caattgcgtc gccgtcgtgt
     2221 tcagccgaga ccggactccc atccggtcaa gctgcgcccg aagcggcgtg agcaagaatg
     2281 aatcgagtcc gtgaggcggg atgatcaact tcgtggcgcg ggcaccggtc aaaaagcagc
     2341 gtcgcagtgt gcgcatgaaa agctctgcgg atacctgcgg aagcgccgcc cccagcaaca
     2401 agcgggcaag gctgttccag acaccgtgcc gggcatgttc cgactggccg atgctcgcga
     2461 gccattcgtc ggccgctcgt gtgtcgagat cgttcggcaa cggaggatcc tgttcccatg
     2521 tgcgttcaag gaaggagagg agatgccagc ggtcccgcat cgacagaccc tggaacaggg
     2581 tggttccgag gagggtgttg agcggggacg gcaggggaag gtgcagaaat tggatgcgag
     2641 ctcccgtcgc ctgaagaaat tcaagcggcg tatgtcgaag ccgcctggtg gcggtgtctt
     2701 tgccgagggt cttgagcagg gaccaggtgg ctgtgtgggc ttcgaggagc aacgggggag
     2761 gcgcgtggca gaggcggcct cccaggtggt cggcctgctc gatcacggtg acggcatggc
     2821 cgtcggcggc gaggtgtaag gctgccgtca gaccggcgat cccgccgccg aggatgagca
     2881 ctgtccggtt catggagtcg aagaggggag acgggagcgc aaccacacgc ctgctgccac
     2941 ggccaaccga tggctcggag tcagcgtgac ccgatctccc aatacgcggt aaccggactg
     3001 ctcgatccgg tgcaagatcc ggctatacac accgcgcatg atttccgcca ccgtcagggc
     3061 ccggcgttcg gcgcgtggca gcgaatcgag cgcccgcgcc gccttggcgt aaaattcctt
     3121 tgctcggccg acttcaaacc gcatgaactc ggtaaattcc ggtttgtacc gccggtgcag
     3181 gagatcctcc tcccggtagg cgaaacgcgc gaggtcttct tgcggcacat agacgcgacc
     3241 gcattctgca tcgttgccca ggtcgcgaag aatattggtc aattgaaagg ccatgccgag
     3301 attgacggca tagtcctggg cgcgcgggga ggtggtgccg aacacgtgca ggcagatcaa
     3361 tcccaccacc gacgccaccc gataacagta gagagacagt tggtcgaagg tggcgtaccg
     3421 ggtggtcgtg aggtccatct cgacgccctt gatgagttcc tcgaaatagg cctgcggaat
     3481 ggacagctcc cgtacgtgcc gggcaaggct gacggtgacg gggaacgtcg gcgtgccgtc
     3541 gtaggcggcg gccagctcgc gccgccagcg ggccaattct tcctgcggat gactgccggg
     3601 aggcggctcg tccacggcgt tgtccacttc cttacagaag gcgtagaccg tgtacatcgc
     3661 ttcgcggcgg gctttcggaa gaaacaaaaa agagtagtag aaattgctcc cgcttttttt
     3721 ggtgagggtc gtgcagtagg tttgggcttc agctggtgtc atcatcgtgt catcagtgtg
     3781 agtgcgtcgt ctgtccctca agtcgttcga cggacgcggg aaggtggcgg ccgtaagagg
     3841 ctgggtgcaa aatggcggcg agttgttcca cgccgtcgat cagtcgtggg ccggggcggc
     3901 tgaagtatga gagcgcatcg accaggtaga cttctcccct ctgaacggcg ggaagatgtt
     3961 gccactgcgg atgcgccgtc atgcttgcga gttcggcttt cgtgcgcggg acggtaaatc
     4021 cgcagggcat gaggacgatc acctccggtt cagaggccgt gagtgtgtcc caatccattt
     4081 ttcgggaggc cgtgccggct tgcgccagaa cgtcgatgcc ccctgcagcg gccaccatgt
     4141 cgggaaccca atggcctgcg gtgtagagcg gagagagcca ttcgatacag gcgactctgg
     4201 ggcgcgttgc ctgagcgccc acggtggccc gtaccgtgtc gagcctgctg cggagcaccg
     4261 cggacaactg tgttccggct tcttcccgcc cgatggctgt tccaatcgtt tcaatatcca
     4321 ggaggatgtc atcaagtcgc ccgggactga gggtgagcag gcgaggcggc ggcgagagtg
     4381 catgaacgac ccgctccaac tgtgacgggg tgatggcgca gacatcacac agcgcctggg
     4441 caatgatcag gtccggtcgt gcggccaaga accgtgtctc atccaattca tagagacctg
     4501 cctcggcggt gagcagcgcg ccgacctgct ggtcgatctc agcgctggag agatgatggc
     4561 tctcgatgcg cggacgcacc atcaccggga tctgtgcgag gctcggcgga aagtcacatt
     4621 catggctgat cccgatcaga tcgtgttggc accccaaggc cgcgacgact tccgtggcac
     4681 ccggcaccag cgaacaaatt ttcatgggcg gccttgagcc gctccccaca ggcgcgcctg
     4741 caggtcctgc agtctggtgt ctgcaagcga atgggtaatg gcatcggcct ctccgagatc
     4801 ctgcaccgtg tgggtattgg cgacagccag cactttcatg ccggcggcgc gtgcggctct
     4861 gatgccgggc agggaatctt cgatgaccag gcagtcgttc gggaacagtg cgggttggtt
     4921 cggttgtgag ttcaagccgg ccatcgcgtg gaggaaggga tcgggcgccg gcttgccacg
     4981 ggtcacgtct tcggcgctgg taatgtgcag aaatgcctta cgaagacccg cctcttccag
     5041 aatcagttcg atttcattcc gcagcgcgcc ggaggcgatg gcgagcggat atcgagcggc
     5101 ggcctcatga accagttcgc gcacgccggg gaagattgcc agatgttgtt tgacggcggc
     5161 caggtaggcg tgggccttgt gctccatcaa ttcgccaagg agcgtgggag acgcctggcg
     5221 ccggtgggac tgcaaggccg ccaggaagca gccgcgatcg tcgaacccga ggtagtcggt
     5281 atagtactcc gcttcggtga gggagatctc aatgccggcc aggacctgtc gcagggcggc
     5341 aaagtgcagg ggctcggtgt cggcaatgac tccgtcaaaa tcaaaaatga tggcgcgtag
     5401 ttcgctcatg gtcgtggttc gggcatccct gtgcgtagtt cattgaatat cgggctgtcg
     5461 gtcgtgtggg cgcggcatta taacaagggc gtatcgacaa gaaaagacga cggcccgtcc
     5521 cactgaggaa cgggccgtca tacgaggcct gagagacgat cgtgggctta gcgtttcagc
     5581 ctgaggctac gttcagggac ccagcctttt cgaccatcct gagctcgaac cgagtagacc
     5641 cagccgcgct tttggtaatc gccgtccagg atcgtgagcg tggcgccggc cagcactgtc
     5701 gtgctggttt tagtccctac ggcgtcaacg aagaggggta tgggggactt gggtctggtc
     5761 ggatcggcga tgaccgtcac ccgttgacct tccgcataga gcggaccggc gacgcgcacg
     5821 gtcggagccg cgggcttggg ggctgaagct gcgggctgga gggccggcat ggcaggtgcg
     5881 ggtattgcgg gagccgacat ggcggacgac ggcgcaggca catcagccgg agccggggtg
     5941 ctgttgtcgg ccaccacggg tggtgccacc gtcggtgctt gtttcggagc ttgagctttg
     6001 accggcggtt tctggatcac gggctgagcg ggctctgcgc cgccacccag atagggttca
     6061 accagctgca tggctccatc cgggtccatg gcgacgtagc cgactcctcc caccagcaga
     6121 agcagcagaa tccagagcaa cggctttttg ccggatcgct tcggcgagtt catcggcggc
     6181 gccgggggcg gcattgattc atccaggtct tcctcggtga attccagatc cggctccggt
     6241 tgacttgcga acaggagatg ccagttcgtc cactcgctgc gcatggcagg aatgcccgta
     6301 gatgaaacca tcggaggacc tcctcattcg aaacaatggc gcgggtctgt ctatttgtag
     6361 gtacttatca ccggccccac agcctgtcaa ttttgttcgt caaagcgaca ggcggcacgc
     6421 atgttctgcg acaaggcccg gccggccggg agtctgtccg acattgaccg ttctactgcg
     6481 gcaaacgatc cacgatcatc tg
//
LOCUS       DAOQRM010000138         6419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_12039_length_6419_cov_15.013199, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000138 DAOQRM010000000
VERSION     DAOQRM010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12039_length_6419_cov_15.013199"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PKK81_10355"
     CDS             <1..321
                     /locus_tag="PKK81_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33325.1"
                     /translation="FVNARIEIGEMMTNYFKGGESYGSGQRPSPEKMKEMGDDINAKL
                     TTLLSKHGLTLEDYRARSKDVFADDAAVKGYLAQHPDLKTRYEALPLNRMSGGGGGST
                     GRGY"
     gene            complement(445..894)
                     /locus_tag="PKK81_10360"
     CDS             complement(445..894)
                     /locus_tag="PKK81_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33326.1"
                     /translation="MTHMTKRLATMATMLAFVASVAGPAMTAAATPEKTSIEIKVRVS
                     EKGFLDEKGRPYGAKRALQVPKDTMVKITFVFGEDMTSLAVGDTHQITIRAEDGWKQE
                     TGSVWVMNRESSVSFLAGEKNRTHYRAYCILDCIGMEHLTNLLIQVV"
     gene            complement(1221..2021)
                     /locus_tag="PKK81_10365"
     CDS             complement(1221..2021)
                     /locus_tag="PKK81_10365"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV33327.1"
                     /translation="MPRAKASSAKRVTLDRLLSKLGIASRSQAQEWIRAGRVRINQRL
                     VRMPDTWIDWPGDAVTIDDRPVQQRPPQFILFHKPKGVVTTHQDEKGRKTIFDVLPPE
                     LKSLHAVGRLDQATSGLLLLTNDSTLSSFLTDPKQQVPRLYLVTVRGEVSDETGRAAL
                     DGVDDQGERLHCASVTIQKRSGRESHLAVTLTEGKNREIRRLFKALGHEVTRLRRIQY
                     GPFTLGDLQPGAWRELPIDAARAQLQLPPAHRARVPQAKPDLLDKRAP"
     gene            2306..2719
                     /locus_tag="PKK81_10370"
     CDS             2306..2719
                     /locus_tag="PKK81_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33328.1"
                     /translation="MVIHPARLMSGILSAVVLCGGTGWAAEPAEVEKFVNARVEVGEL
                     MTSYFQGWERYGEGQRPSPERMEEMTVEINAKLEPLLAKYDLTVESYRERIKDVLADE
                     VAVKGYLSQHPDVKKRYDALPFDRMRRDGGSGRGY"
     gene            2778..3170
                     /locus_tag="PKK81_10375"
     CDS             2778..3170
                     /locus_tag="PKK81_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33329.1"
                     /translation="MLKYVVLVMFSTLLFCNVASAEWILVGGTHKYDGYVDVATVGPT
                     GKTVTLWTLKDFKVDRQIPQGTYRSVRIKKQIDCLAHKSRPLQTKYYATQMGKGRPLQ
                     SGKGTREWSRVTPGSDGEAELKIACKTV"
     gene            3236..4486
                     /locus_tag="PKK81_10380"
     CDS             3236..4486
                     /locus_tag="PKK81_10380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV33330.1"
                     /translation="MSANPRVIIVGAGLAGLACARHLSQAGFACSLLEASDAVGGRVR
                     TDRVDGFQLDRGFQVFLTGYPEAGRMLDYPALQLQPFHPGAMVRYAGGFHRISDPLRR
                     PQDLLSTMMSPIGSFGDKLKVLRLRRDALRRQLCGQAGGGERTTLEALQVYGFSPAMQ
                     QRFFQPFLNGVFLDQALSTPCRLFELVWAAFSRGDIALPQQGMGAIASQLAATLPTES
                     IRLNAAVSHLDGTTAVLASGERLAADVLVLATDDATAATLRGDSRPAAPGRDAICLYF
                     DAPAPPVRGPWLILNGDGQGPVGTACALSEVASGYAPLGRSLISVSLSGQPSLAGGDL
                     QQAVRRQLREWFGEEVHVWRHLRTDHIKRALPPIEVLTAQASGASPRVRPGLYQCGDY
                     CETGTLDGALLSGRKAAEALLADH"
     gene            4512..4979
                     /locus_tag="PKK81_10385"
     CDS             4512..4979
                     /locus_tag="PKK81_10385"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV33331.1"
                     /translation="MNGRGARVTNHFGWRWLLAATMVLLSCPLPSALADPSSQHQLQA
                     LAGQGDANAQWMLGQALLTGSLGETDEAEAVRWLQLAADQGHALAQRDMGMLYEQGQG
                     VTQDVLEAFFWYSLASRQDSSRARLRRDALAAMLTAEQHEAIAARLKGWRPRK"
     gene            complement(4997..5488)
                     /gene="def"
                     /locus_tag="PKK81_10390"
     CDS             complement(4997..5488)
                     /gene="def"
                     /locus_tag="PKK81_10390"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNV33332.1"
                     /translation="MAIRPILQYPHQALKSTNAAVAPSDPAVQAVVQDMLDTLAASPG
                     VALAAPQIGHAVQVIVVDVSRKKGEGGHGLVVLLNPVILSLEGKKSVREGCLSVPDYT
                     GHVLRYEQALVEGMAPDGRVVTVSASGFEALAFQHEVDHLNGMLFLDRIQSLSTDLFR
                     RKK"
     gene            complement(5491..6039)
                     /locus_tag="PKK81_10395"
     CDS             complement(5491..6039)
                     /locus_tag="PKK81_10395"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNV33333.1"
                     /translation="MNHFAIVTAFGQDRPGIVALMADSLYRLGCNIEDTCMTRLRGEF
                     TMMLMVRLPEGIAAEELGRRLTPYTTPLDLAVLCRAMPDQAATRQTALEVPTFMLSVY
                     GADHPGIVAQVARTVAQHNGNITDMNTRVVGSGERPIYVMVLEVQLQEAQQADQLRQA
                     LEQLKPLLGVDLTFRLLESVTF"
     gene            complement(6155..>6419)
                     /locus_tag="PKK81_10400"
     CDS             complement(6155..>6419)
                     /locus_tag="PKK81_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HNV33334.1"
                     /translation="ETPVLAVRLQEMFGCQETPRIAGGRVPVMVHLLSPAGRPVQVTK
                     DLASFWRSTYQEVKKELRGRYPRHHWPDDPLTAQPTNRTKRRT"
BASE COUNT         1155 a         1792 c         2131 g         1341 t
ORIGIN      
        1 tttgtgaacg cccgcatcga gatcggcgaa atgatgacga attatttcaa gggcggggag
       61 tcctacgggt ccgggcagcg gccgtcgccg gagaaaatga aggagatggg cgacgacatc
      121 aacgcgaaac tgaccacgct cctgtccaag catggcctga cgctggagga ttaccgcgcg
      181 cgcagcaaag acgtgttcgc cgatgatgcg gcggtgaaag gatatctcgc ccagcatccg
      241 gatctgaaga cccggtatga ggcgctgccg ttgaatcgga tgagtggggg tggaggaggc
      301 agcaccggac ggggctacta gcaggcgggt gaatcctcac cactctgcgt gctcacaaat
      361 tcgaccgctc ggcgtattgc atcagtacgc cgagcgtttt catttgaccg cggctttgcc
      421 ggacaggcta gttgaccagc ctggttagac gacttggatc agcagattgg tgagatgctc
      481 catgccgatg cagtcgagga tgcagtaggc gcgatagtga gttcggttct tttccccggc
      541 cagaaaactc acgctcgatt cccggttcat cacccagact gagccggtct cctgcttcca
      601 gccgtcttcg gcccggatcg tgatctggtg cgtgtctccc acggccaggc tggtcatgtc
      661 ttcaccgaac acgaaggtga tcttcaccat cgtatctttc ggcacctgca gggcgcgctt
      721 cgctccgtag ggtcggccct tctcatccag gaatcccttt tcactgactc gaaccttgat
      781 ctcgatcgag gttttttccg gggtggccgc agcggtcatc gccggtccgg cgacgcttgc
      841 gacaaacgcc agcatggtgg ccatggttgc caagcgcttt gtcatgtgag tcattgctct
      901 cgtgctcctc tatttgagac cgcctgcgat cgcaggtgtg ccgttcccct gtcatagggt
      961 ggctgcgagt cttgctggtg gtggactaca gctgggggcg actcttacga caatgcggaa
     1021 ggtcacgctc cgaaatttcg tgtgctgtat tcaccatagc acaggcaagc aggttggcaa
     1081 gttctttcac ccgctccggc aaagagaacg acaggcttga tggaaaaaac gtagggagtc
     1141 agcgacggcg cccacttttc agtgagttgt cggccctggc ctggaaagat ttcagctgac
     1201 gtgtaggggg gagcgggcgc tcagggtgcg cgcttgtcga gcaggtctgg tttcgcctgt
     1261 ggtacccgtg ccctatgagc cggcggcaac tggagttgcg ccctggctgc gtcgatcggg
     1321 agttcgcgcc aggcgcccgg ttggagatcg ccgagggtga aggggccgta ttgaatccgc
     1381 ctcaatctgg tcacctcatg tcccagcgcc ttgaagagcc gccggatctc acgattcttg
     1441 ccttcggtca acgtgacggc cagatgggat tcccggcctg agcgtttctg aatggtgacg
     1501 gaggcgcagt gcaaccgctc gccctgatcg tccaccccgt caagcgctgc ccggcctgtc
     1561 tcgtccgaga cctcaccacg caccgtgact agatagaggc gcggcacctg ctgtttcgga
     1621 tcggtcagga agctggagag cgtcgagtcg ttggtgagca ggaggagacc actcgtcgcc
     1681 tgatccaagc gaccgactgc gtgcaggctt ttcaactcag gaggaagtac atcgaagatg
     1741 gtctttcggc ccttctcgtc ctgatgggtc gtgactacac ctttgggttt atggaagaga
     1801 atgaattgcg gagggcgctg ctggaccggt cgatcgtcga tggtgacggc atcgccgggc
     1861 cagtcgatcc aggtgtccgg catgcgtacc aggcgttgat tgatgcggac tcgtccggcc
     1921 ctgatccatt cctgtgcctg gctccggctg gcaatgccga gtttggagag cagtcgatcc
     1981 agcgtcacac gtttcgcgct gcttgccttc gcgcgtggca tgctccgcgc cggctttcgg
     2041 ggaggaggca ccctgtcgga cgacgccgga tctcgttgtg tgaagtgcgg tttgccggtc
     2101 atgctcatcg ggccaagcgg agggccacgt ctggatgcgg tcgcatggtg tgactgtaca
     2161 ctgccgagtg cggaaagaga agtcgatcga tgacagctgc tgtggagcgt ggggccggga
     2221 tcagttggat gtatgtcgca tacctgtgat ggtatgatgg ctgcggcttt gcagcgaacg
     2281 agggaccatt cttcgagagg gcaccatggt gatccatccg gcgcgtctga tgagcggcat
     2341 cctgtcggcg gtggtcctgt gcgggggaac cggttgggct gccgagccgg cagaggtgga
     2401 gaagtttgtg aacgcgcggg tcgaggtcgg tgagctgatg accagctact ttcaggggtg
     2461 ggagcgatat ggagaggggc agcgtccgtc gcccgagcgg atggaggaaa tgacggtcga
     2521 gatcaatgcc aagctcgagc cgctcctggc taaatatgat ctgacggttg agagctatcg
     2581 tgagcggatc aaagacgtat tggccgatga agtcgccgtc aaaggctatc tctcccagca
     2641 ccccgacgtg aagaagcggt atgacgctct gccctttgat cgtatgaggc gtgatggagg
     2701 cagcggccga ggttattagc aggaagatag cccgaggctt ctggaacgtg tatctgaacg
     2761 cttgtgatgg ggcaaacatg ctgaaatacg tggtgctggt gatgttctcg acgctgcttt
     2821 tttgcaacgt ggcgtcggcg gaatggattc tggtcggcgg cacgcacaag tatgacggct
     2881 acgtggatgt ggccacggtc ggtccgacgg gcaagaccgt gacgctctgg accctgaagg
     2941 acttcaaagt ggatcggcag attccgcagg ggacctatcg ctccgtgcgc atcaagaagc
     3001 agatcgattg tctcgctcac aagagccgtc ccctgcagac caagtactat gcgacacaaa
     3061 tgggcaaggg tcgtcctttg cagtcgggga aaggaaccag ggaatggtcg cgggtgacac
     3121 ccgggagtga cggggaggcg gagttgaaaa ttgcctgcaa gacagtctag caggctgaga
     3181 atcgtcgtca ggaaactgga gtctggtccc gcccgcgttt agaatgccgc ccatcatgtc
     3241 tgctaatcct cgtgtgatca tcgtcggcgc cggtcttgcc ggcttggctt gtgcgcgaca
     3301 tctgtcgcag gccggattcg cctgtagcct gcttgaggca tccgatgccg tgggcgggcg
     3361 ggtgaggacc gatcgtgtgg atggattcca acttgatcga gggtttcagg tgtttctgac
     3421 cggttatccc gaagccgggc ggatgttgga ttatcccgcg ctgcaactcc agccgtttca
     3481 ccccggcgcg atggtgcggt atgccggcgg ttttcatcgg atcagcgacc cgcttcgccg
     3541 tccgcaggat cttctctcca cgatgatgag cccgatcggt tcgttcggtg acaagctcaa
     3601 ggtgctccgg ctccgtcggg atgcgttgcg ccggcagctc tgtgggcagg cgggcggagg
     3661 cgagcgcacc acgctggagg ccctgcaggt ctatgggttt tctcccgcca tgcagcagcg
     3721 ctttttccag ccgtttctga atggcgtgtt tctggatcag gcgttgtcca cgccctgccg
     3781 gctgtttgaa ttggtctggg cggcgttctc tcgcggcgat attgcgctgc cgcaacaggg
     3841 catgggcgcg attgcgagcc agctggccgc gactcttcca acggagtcga tccgattgaa
     3901 cgcagcggtc agtcacctgg acggcaccac cgcggttctg gcgtccggtg aacgactcgc
     3961 cgccgatgtt ctggtcctgg ccacggatga tgccaccgct gccacgttac gaggcgatag
     4021 ccggccggcc gcgcccggac gtgacgcgat ctgtctgtat ttcgacgcgc ccgctccacc
     4081 ggtccgtgga ccctggttga ttctgaacgg agacggtcag ggaccggtag gcaccgcctg
     4141 tgccttgagc gaggtcgcct ccggttatgc gcccttggga cggtcgctga tttcagtcag
     4201 cttgtcgggg caaccatctc tcgccggcgg tgatctccag caagcggtgc gccggcagtt
     4261 gcgcgagtgg tttggtgagg aggtgcatgt ctggcgacac ctccggaccg atcacatcaa
     4321 gagagcgctg ccgccgattg aggtcctcac ggcgcaggca tcaggcgcat caccgcgcgt
     4381 gaggccgggg ttgtatcaat gcggtgatta ttgcgagacc gggacgttgg atggggcgct
     4441 gctgtccggg cgaaaagccg ccgaggcgtt gttggctgac cattgagtag agtcgcagac
     4501 ggggggcggt cttgaacgga cggggagcgc gggtcacgaa tcatttcgga tggcgatggc
     4561 tgttggcggc gacgatggtg ctgttgtcct gtccgctgcc gtcggccttg gccgatccgt
     4621 cctcccagca tcagctgcag gcgcttgccg ggcagggcga tgcgaacgcg caatggatgt
     4681 tgggacaggc cttgctcacg ggcagtctcg gggaaaccga tgaggccgaa gcggtgaggt
     4741 ggctgcagtt ggcggccgac cagggccacg ccttggcgca acgcgacatg ggcatgttgt
     4801 atgagcaggg gcagggggtg acgcaggatg tgctggaggc gttcttctgg tattcccttg
     4861 cgagtcgtca ggacagcagt cgggccagac tccgccgtga tgcgttggcg gcgatgctca
     4921 ccgcggaaca acatgaggcc attgcggcac gtctcaaggg atggcggccg agaaaataat
     4981 tggtcgttgt cgggggttat ttttttcgtc tgaagagatc ggtgctgagt gactgaatac
     5041 ggtcgaggaa gagcatgccg ttcagatggt ccacttcatg ctgaaaggcg agcgcctcga
     5101 atcctgacgc actcaccgtg acgacccggc cgtccggcgc catcccctcc acgagcgcct
     5161 gttcgtagcg cagcacatga cccgtatagt ccggcacgct caggcagcct tcccgcacgc
     5221 tttttttccc ttccagcgag agaatgaccg gattcaacag cacgaccaat ccatggcccc
     5281 cctcgccttt cttgcgcgac acatccacga cgatcacctg cacggcatgc ccgatctggg
     5341 gcgccgccag tgccacgccg ggtgacgcgg cgagcgtatc cagcatatct tgaaccaccg
     5401 cttgcacggc cggatcggag ggggcgacgg cggcgttcgt ggatttgagt gcctgatggg
     5461 gatactgcag aatcggacgg atagccacga tcagaaggtg acactttcga gcaggcggaa
     5521 ggtcaaatca acacccagca gtggcttcaa ttgttccagg gcctgtctga gctggtcggc
     5581 ttgctgggcc tcctgtaatt gaacctccag gaccatgaca tagatgggac gctctcccga
     5641 tccaaccacg cgggtattca tgtcggtgat gttgccgttg tgctgggcga cggtgcgggc
     5701 cacctgtgcc acgatgcccg gatgatcggc cccgtaaacc gacaacatga acgtcggtac
     5761 ctccagtgcc gtctgcctgg ttgctgcctg atccggcatc gctctgcaca agacggcaag
     5821 gtccagcgga gtcgtatagg gcgtcaggcg tcggccaagc tcctcggcgg cgattccctc
     5881 tggaaggcgc accatgagca tcatggtgaa ctcgccacga agccgggtca tgcaggtgtc
     5941 ttcgatatta cagccgagtc ggtagaggct atccgccatc agggcgacga taccgggacg
     6001 gtcttgcccg aaggcggtga caatcgcgaa atgattcatt ggcatctcgt gggtcgcaag
     6061 agtgggtgca ggctgcgagt agaatgctgc aattgtggaa gattagcaag cgccggtcgc
     6121 acgtgcgtgg ttggacaagc ggaagggccc ggtcctacgt tcgtcgtttt gtccgattcg
     6181 tgggttgagc ggtgagtgga tcatccggcc agtggtggcg tgggtagcgg cctcgcagtt
     6241 ctttcttcac ctcttgataa gtcgagcgcc aaaagcttgc caggtctttc gtgacctgga
     6301 caggccgtcc cgccggcgag agcagatgca ccatcaccgg cacccttcca cccgcgatgc
     6361 gcggtgtctc ctgacagccg aacatttcct gtaagcgcac ggccagcacc ggcgtctcg
//
LOCUS       DAOQRM010000139         6372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_12170_length_6372_cov_14.099889, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000139 DAOQRM010000000
VERSION     DAOQRM010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12170_length_6372_cov_14.099889"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKK81_10405"
     CDS             <1..788
                     /locus_tag="PKK81_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33335.1"
                     /translation="VIDLSVGHVDFRETGPYGPGHWIVQPDMGCAPCGFDQVCLHHAC
                     KDRLVPDQIAALCLHVLNGGAFPEKLTGIKIYRSRVDEDGLGSTELHAGREDPTVSWY
                     GRFWRRFWFEQFTGRPSLVPMVSEPPPDWKESMALLDRLMPLAARLVSRAEELVRLTA
                     RRPLPISTLQQMQLEESGERQTVVALSLQSPATASLAVALVRDTHNDDGHELTGMAEC
                     RLATYRRWRKRLHTVAAGFRSFNTAQGFAPRGTTVAIPMARIG"
     gene            833..1375
                     /locus_tag="PKK81_10410"
     CDS             833..1375
                     /locus_tag="PKK81_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33336.1"
                     /translation="MHITLDGEQWQIPDDTSLMNALAFLSDKAHAQHRIVTSLTVGGK
                     AISDRDLDPAFLNQRGQDVGEVSGRSQSLHAIITDAKQAIDRFAAQLRADGLVLLGPL
                     RSGTGQVGTIDAWLGRLADYTEMLEAGQAQGVAGLSSAPLLPWIQELLGARTFADPIR
                     VADLLEYELLPLLTESSQAA"
     gene            1613..2440
                     /locus_tag="PKK81_10415"
     CDS             1613..2440
                     /locus_tag="PKK81_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV33337.1"
                     /translation="MALVVNTNVSSLAAQRNLSINQAQLGRSVERLSSGLRITRAADD
                     AAGLGVSETLRAQIRSINQANRNAGDGISLTQVADGAAATVGSLLSRMRELATQSASG
                     TLGATERSYLDQEFVALRSEIDRIATTTEYNGQPLLSGSSNTFEVFIGFKSGSGNSLN
                     VALADLDVAAVGLTGASVSTAAAAQTMLSNIDSAISAVATARANYGSIQSRFEVAIQN
                     LTVTAENFTAAESRIRDADIAQETSVFTKNQILTQSGIAILAQANSLPQQALALLRG"
     gene            2508..2879
                     /locus_tag="PKK81_10420"
     CDS             2508..2879
                     /locus_tag="PKK81_10420"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNV33338.1"
                     /translation="MVHQVSNHKDLPAAATHASQSGPQHAVDKKAEAQRPEPETSTTE
                     VKGEVKGKDLEQALSRVREVFQKADSRLEFSVDPDLDRVVVKVMDGDSGTVIRQIPQQ
                     EVIDLAKRLETPTGLLLHHKV"
     gene            2907..4298
                     /gene="fliD"
                     /locus_tag="PKK81_10425"
     CDS             2907..4298
                     /gene="fliD"
                     /locus_tag="PKK81_10425"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNV33339.1"
                     /translation="MAISFGGLGNGVDFGQVVSELAKVQRLPIDALTAKKKDLQTKLT
                     DYGALGNKLLALQSAANALRLPSSFNRSTSTVSDDNILTAQAGAGAATGSYTVQVTQL
                     AKANQITNKAVKAVSGTTEVVSSGAGTFTFRVGSGTDQTVTLSAGATLDDLRSAINDL
                     GAGVTASVINTGTEASPAYRLTLTATASGAANTITVVTDTTTLDFTNTTGTGGNDTLQ
                     AGQNAIVVIGDPDQTTISIERASNVISDAIPDVTLTLKSKTVTTPDPEPVTVNISNDP
                     ASVKTNIKTLVTAYNDIVKFVNDRTGYDITTKTGGIFFNESTAKNVLSQLRTAISGEV
                     SELSTYKSLGAVGFKTERDGTLTIDDAKLDSAMASNYVATRALFVTQTTSSGIADRIV
                     KAVDFLDSVDSGAFTVRKNSITSQISRLTDQIGRKEDIASQYEERLRLQFASLDGLLQ
                     KLQSQTSALQALR"
     gene            4313..4705
                     /gene="fliS"
                     /locus_tag="PKK81_10430"
     CDS             4313..4705
                     /gene="fliS"
                     /locus_tag="PKK81_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNV33340.1"
                     /translation="MIATAANAYQQTQVMTASRVQLIVLLYDSAIQSMELAREAILTN
                     HYKDKARFLDRSIAIVGELSSVLDFERGGEIAVSLHRLYDYMVQQCIQANLRHNGKHL
                     DGPIRCLTTLREGWHVVARQEAVAHVGS"
     gene            4692..5036
                     /locus_tag="PKK81_10435"
     CDS             4692..5036
                     /locus_tag="PKK81_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33341.1"
                     /translation="MSAASSLDTDPLHDHPSIERLTIQALEAARAGDWDQVDACYSAR
                     GIRLAACELDRTVAQQLLRMDEEVRAAILVAQAGISGQLADAAQVKRHLRQLRESSGQ
                     LASERVTIDREA"
     gene            5094..5705
                     /locus_tag="PKK81_10440"
     CDS             5094..5705
                     /locus_tag="PKK81_10440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV33342.1"
                     /translation="MTPSHDRFRQAPRTDIKNEERREWLRIEDRLLLEYRRFDEPAEA
                     MNAHLPPATEDTIATAVSKPTIDLLVRAGETFAGSPLLPWVSKIDWMLETILKSLVKS
                     HPGSVAIARLTDVDISAGGLGFDTPRRFQADDLLVLKVILPPFSMIEATARIIRVTPA
                     EKETTGFHVATQFVELGGDEQELIIRHILQVQAERLRARQAAH"
     gene            complement(5758..>6372)
                     /locus_tag="PKK81_10445"
     CDS             complement(5758..>6372)
                     /locus_tag="PKK81_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33343.1"
                     /translation="LIRHFRQLQADVIIVDIGAGTSYHALDFFLMADHYLTVATPDPT
                     SVLDLYRFIKLAAIRRVLSAFLSRDAVAETLSDRDFCSIDEVIQAVSETDPNAKETAS
                     RTLRGFHPYLIVNRVSGKSRVNVLQLKKLLQEYVGGDLMMLGEIPDDPAMTRAVRSYL
                     PVVEFEPTAPAALALEKAAQTLLTLVARPVTIPTETAPAPEQAA"
BASE COUNT         1401 a         1868 c         1880 g         1223 t
ORIGIN      
        1 cggtgatcga tctctcagtc gggcatgtcg actttcgcga gacgggtccc tatggaccgg
       61 ggcattggat cgtccagccg gatatggggt gcgcgccctg cggattcgat caggtctgtt
      121 tgcaccacgc ctgcaaggat cggctggtgc cggatcaaat tgccgcgctg tgcctgcatg
      181 tgctcaatgg aggcgcattt ccagaaaaat taaccggcat caagatctat cggtcccgcg
      241 tggatgagga cgggctggga agcactgaac ttcatgcagg acgggaagat cccacggtca
      301 gctggtatgg ccgattctgg cggcggttct ggttcgagca atttacgggt cgtcccagtc
      361 tggtacccat ggtttcggag cctccgcccg actggaagga gagcatggca ctcctggatc
      421 ggcttatgcc tctcgcggcc aggctggtat ccagagcgga ggaactggtt cgtttgaccg
      481 cccgacggcc gttgccgatc agtaccctgc agcagatgca gcttgaggag agcggggagc
      541 gtcagacggt tgtggcgctc agtctccaat ccccggcgac cgcatccctg gcggtggccc
      601 tcgtgcgaga tacgcacaat gacgacggcc acgaactcac cggcatggct gaatgccggc
      661 tcgcaaccta tcgacgctgg cggaaacgac tgcatacggt cgcggccggc ttccgctcgt
      721 tcaacacagc ccaggggttc gcaccgcgtg gaaccacggt agccattccc atggcgcgta
      781 tcgggtagcg gcagaagacg tacgtttgac actcggctaa aggagtccga tcatgcatat
      841 caccctcgac ggcgaacagt ggcagatacc ggatgacact tctctcatga acgccctggc
      901 gtttctcagc gacaaagctc atgcccaaca tcgcatcgtg acgtcgttga cggtcggcgg
      961 gaaggccatc agcgatcgcg atttggatcc ggcctttttg aatcaacggg ggcaagatgt
     1021 gggagaggtg tccgggcggt ctcaatcgct gcacgccatc attacagatg cgaaacaggc
     1081 catcgaccgg tttgccgcgc agttgcgagc cgatggcctg gtcctgctcg gcccgctccg
     1141 ctcgggaacc gggcaggtgg gaaccatcga tgcctggctg ggccgtcttg cagattatac
     1201 ggaaatgctg gaagccggtc aggcacaggg tgtcgcagga ctgtcatccg caccgctcct
     1261 cccgtggata caagaactcc tgggcgcgcg aaccttcgcc gaccccatcc gggtcgcgga
     1321 tctgctggag tatgaattat tacccctgct cactgaatcc agtcaggctg cttagtgctc
     1381 attgctctcc gataggacac ctcaacggat gatgcctccc attgcacccg ttttctgcct
     1441 ttcatggcgc tcttaagtct tcccgacagc ctaccgataa ggggcacagc aatacggtag
     1501 caaggcgcag cggggatgtg gccacatctt caccagacct ggttaccacc tcgtcggcac
     1561 ggacgctggc gactttccgg tagtagtgcg gaagcttcag gaggaagtag tcatggcatt
     1621 agtcgtcaat accaacgttt catctctggc agcccagcgt aacctttcga tcaatcaagc
     1681 ccaattgggg cgttcagttg aacgattgtc ctcgggcttg cgaatcaccc gtgcggcgga
     1741 tgacgccgcc ggattaggcg tgtccgaaac attgcgggcg cagatccgca gtatcaatca
     1801 ggccaatcga aacgccggtg acggcatcag cttgacccag gtcgccgacg gtgccgcggc
     1861 cacggtcggc agcttgttgt ctcgtatgcg tgagctggcg actcagtctg ccagcgggac
     1921 gctgggagca accgagcgct cctacctgga ccaggagttt gttgcgttac gttcggaaat
     1981 cgatcgaatt gcgacaacga ccgaatataa cggtcagccg cttctgagtg gaagcagcaa
     2041 cacgtttgaa gtctttatcg gtttcaaaag tggatcgggc aattcgttga atgtggcatt
     2101 ggccgatctc gacgtcgcgg cggtcggatt gaccggtgcg agtgtgtcga cggcggcagc
     2161 cgcacagacc atgctttcca atatcgacag cgccatcagc gcggtggcaa ccgcccgcgc
     2221 gaattacggg tcgattcaaa gccggttcga agtcgcgatc cagaacttga cggtcacggc
     2281 tgagaacttt actgcggcgg aatctcggat tcgagacgcg gacattgccc aagagacatc
     2341 tgtgttcacg aagaaccaga tcctcaccca atccggcatc gcgattttgg cgcaagccaa
     2401 ctcgttgcca cagcaagcct tggcgctgct gcgaggataa ggcatcagcc gccggatagc
     2461 cctcccgtat gggagggcta tccacggatt gaaccgagga gggtaacatg gtccaccagg
     2521 tttcgaatca caaggacctc cccgcagccg ccacccatgc gagccagagc ggacctcaac
     2581 atgccgttga caagaaggct gaggcccagc gccctgaacc tgagacgtcc acgactgagg
     2641 tcaaagggga ggtcaaagga aaggatctgg aacaggctct gtcgcgagta cgtgaagtgt
     2701 tccagaaggc agactccaga ttggagttta gcgtcgatcc ggatctggat cgggtggtgg
     2761 tcaaggtcat ggacggggat tccggaaccg tgatccgcca gattccgcag caggaagtca
     2821 tcgatcttgc gaagaggttg gagacgccga ccgggctgct cctgcaccac aaggtgtaac
     2881 ctgcttcacg acatgaaagg gatgtcatgg cgattagttt cggcgggtta ggaaatggtg
     2941 tggactttgg tcaagtcgtc tcagaattgg ccaaagtcca acgcctgccc atcgacgcat
     3001 tgacggcgaa gaaaaaggat ctgcagacca aactcaccga ttatggcgcc ctcggcaaca
     3061 agctgctcgc gcttcagagt gccgcgaatg ccctccggct tcccagcagc ttcaatcgat
     3121 ctaccagcac cgtcagcgat gacaacattc tcacggctca agccggcgcg ggagcagcga
     3181 ccggcagtta tacggtgcaa gtcactcagc tggccaaagc caatcaaatt acgaacaaag
     3241 ccgtcaaggc ggtctccggc accactgaag tagtctcgag cggcgccggc acctttacct
     3301 ttcgggtcgg aagcggcacg gatcaaacgg tcaccctcag cgccggcgcg acactcgacg
     3361 atcttcgttc tgcgatcaac gatctgggag ccggtgtgac cgcctccgtg atcaatacgg
     3421 gaacggaagc aagcccggcc tatcgactga cgttaaccgc aaccgcatcg ggcgcggcca
     3481 ataccatcac cgtggtgacc gatacgacca ctcttgattt cacgaacacg accgggacgg
     3541 gcggcaacga tacgctgcag gcagggcaga atgccatcgt cgtgatcggt gatcccgacc
     3601 agaccacgat ttccatcgag cgcgcgtcca acgtcatctc cgatgcgatt ccggacgtca
     3661 ccctcacgct caaatcgaaa accgtcacga ctcccgatcc ggaaccggtc acggtgaata
     3721 tcagcaacga ccccgcgagc gtcaagacca atatcaaaac cctggtgacc gcctataacg
     3781 acatcgtgaa gttcgtcaac gatcgaaccg ggtacgacat cacgacgaag accggcggga
     3841 tcttctttaa cgagagcacg gcgaaaaacg tcttgagcca gttgcgtacg gccatctccg
     3901 gagaggtcag tgaactctcc acctataagt cgcttggcgc ggtcggattt aaaacggaac
     3961 gagacgggac actcactatt gatgacgcca agttagactc ggcgatggcg agcaactatg
     4021 tcgccacacg ggccttgttt gtgacgcaga cgacctcgag cggcattgca gaccgtatcg
     4081 tgaaagcggt cgatttcctc gacagcgtcg acagcggcgc cttcacggtt cgaaaaaact
     4141 ccatcaccag ccagatcagc agactcaccg atcaaatcgg ccgcaaggaa gatatcgcct
     4201 cgcaatatga ggagcggctg cgacttcaat tcgcctcatt ggatggattg ttacagaaac
     4261 tacaatcaca aaccagcgcc cttcaggcgc tcagataagt tataaggtca ttatgatcgc
     4321 caccgccgcg aacgcctatc aacagactca agtcatgacc gccagccggg tgcaactgat
     4381 cgtgctgctg tacgattccg cgattcaatc gatggagctg gcccgggaag ccattctgac
     4441 gaaccactac aaggacaagg cccgttttct cgatcgcagt atcgcgattg tcggggaact
     4501 gtccagcgtc ctggacttcg agcgaggcgg ggagatcgcg gtctcgttgc accggttgta
     4561 tgactacatg gtgcagcagt gtatccaggc caaccttcgg cacaacggca agcacctgga
     4621 cggcccgatt cgctgcctga cgacactacg agaaggttgg cacgttgttg cgaggcaaga
     4681 agcggtggcg catgtcggca gctagttcac tcgataccga tcctctccac gatcacccct
     4741 ccatcgaacg gctcaccatt caagctctgg aggctgcccg ggccggcgac tgggatcaag
     4801 tcgacgcctg ctatagcgcg cgcggaatca ggctggccgc ctgcgaactc gatcgcacag
     4861 tcgcgcaaca actgctgcgc atggatgaag aagttcgcgc ggccatcctg gtggcgcagg
     4921 caggaatatc cggccaattg gccgatgccg ctcaagtgaa acgacacctg cgccaattac
     4981 gtgaaagtag cggacaactg gcttccgaac gtgtcacgat cgatcgtgaa gcctgacgcg
     5041 cttgagcccg ggcgacatgc ctcggtcgct ttcctccacc gcagaggttc ggtatgactc
     5101 cgtcacatga ccggtttcgt caggcccccc gcaccgatat caagaatgaa gagcgtcgcg
     5161 aatggttgcg catcgaagat cgcttgctac tcgagtaccg tcgtttcgac gaacctgccg
     5221 aagccatgaa cgcgcatctg ccgccggcga cagaggatac gattgccacc gcggtgtcaa
     5281 aaccgaccat tgatctgctg gtccgggcag gggagacctt tgccggctcg ccgctcttac
     5341 cctgggtctc caaaatcgac tggatgctgg agacgattct caagtccctc gtgaaaagtc
     5401 atcccgggag cgtcgccatc gcacggctga ccgatgtcga tatcagcgcc ggcgggctgg
     5461 gcttcgatac cccgcgtcgg tttcaggcgg atgatctgtt ggtgctgaaa gtgatcctgc
     5521 cgccgttcag catgatcgag gcgacggccc gcatcattcg agtgactccg gccgaaaagg
     5581 aaacgaccgg gtttcatgtc gccacacagt ttgtcgaatt gggcggggac gagcaggagc
     5641 tgattatccg gcatattctg caggtgcaag ccgaacggct gcgggcaaga caagcggcac
     5701 actaacggcc gtatcgcggc gcttcgcgat gaacctgcat ctggtagttt ccggaggtta
     5761 tgcagcctgt tcaggcgccg gagcggtctc agtcgggatc gtgaccgggc gagctaccag
     5821 ggtgagcagg gtctgagccg ctttctcgag ggccagcgct gcgggtgccg tgggttcaaa
     5881 ttcgacgacc ggaagataac tacgcacggc acgtgtcatc gccggatcat ccggaatctc
     5941 ccccagcatc atcagatccc cgccgacgta ttcctgcaac agtttcttca actgcagtac
     6001 attcacacgc gactttccag acacccgatt cacgatcaga taggggtgaa atccacggag
     6061 tgtccggctg gcggtctcct tggcgttggg atcggtttcg ctgaccgcct ggatgacctc
     6121 gtcgatactg cagaaatccc gatcagagag ggtctcggcg acggcatctc gcgagaggaa
     6181 ggccgacagg acacgacgaa tcgcggccag cttgataaag cggtacaggt cgagcaccga
     6241 cgtcggatcc ggagtggcca cggtcaggta gtggtccgcc atcagaaaaa agtccagcgc
     6301 atggtaactg gtgcctgcgc cgatatcgac aatgatgaca tcggcttgca gttgccggaa
     6361 atggcgaatc aa
//
LOCUS       DAOQRM010000140         6345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_12253_length_6345_cov_20.837679, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000140 DAOQRM010000000
VERSION     DAOQRM010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12253_length_6345_cov_20.837679"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PKK81_10450"
     CDS             complement(<1..1151)
                     /locus_tag="PKK81_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV33344.1"
                     /translation="MFRWGLYSLRNLWARRVTTALTVVGLGLVVFVLLAVLMLAHGLE
                     QTLGKTGDPENVIILRKGALSEIGSSLSREEIRAVAAQPDVRLTADGRPLAVAEVGLQ
                     LTLRKGQGGSLTSVTLRGTAPEALAVRPAILLIQGRLWKPGTTEIVVGASIARQFPDA
                     RVDRVLHFGNRAWTVVGVFEAGGSGFDSEVWGDAEQFLATFHRSNFSSLTLRLAQPAT
                     LPAFKDRIERDPRFKFSVKREPEYYEGKAEGLARMIRVTGLFLTVVFSVGALLGATMT
                     MSASVAQRTTEIGTLRTIGFTRMDILQAFLLESMALGAIAGLLGVAAAVGLQSMTIST
                     MNFDTGTELVFGFHLSGEMIAIALVFAMLMSIVGGLIPATRAARLVIVQA"
     gene            complement(1350..2516)
                     /locus_tag="PKK81_10455"
     CDS             complement(1350..2516)
                     /locus_tag="PKK81_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV33345.1"
                     /translation="MTLGRLVLRNTLRRPVRLALTVWGLAMVVLAFGLIRLTLDEWQG
                     AAKAASKNRLITVHAMSGALPLPLAYRDRMAVLPGVRSVHYGVEFGGIYKDPKQPLAS
                     FAEPAATLLTTYPEILLSEGERLGFVQDRRGCIVGLSLAERYGWKLGDVIPLTGTSYP
                     GQWEVIVRGIYRSSNLMIWSDQELFLQWEYLNEQVKTTQPDRADQVSWYAIDVKEGSN
                     PRAVALALDALFANSFAETRTELEQAFIAGWVERSSALLYGLEAVSAVINGIALLVLV
                     NALAMAVRERTREYAVIKTLGFQPRHFCILVLGESLLLALSGGALGLGLLVPASRLYA
                     QMTQSNGYVATYAVSMETVGLCLGMMVLVALGAAIWPAVRLIRMTTLDGLRHAG"
     gene            complement(2513..3196)
                     /locus_tag="PKK81_10460"
     CDS             complement(2513..3196)
                     /locus_tag="PKK81_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV33346.1"
                     /translation="MYDPVVTLQRISKTYSRAGTDVSVLRDLSFTIPAGTFLAIMGPS
                     GSGKSTLLNVMAGIDRPTSGSVVVAGTRLDDLSEGDMARWRARHIGYVFQSYNLIPVL
                     TASENVELPLLLTHLSRQERREHVKTALRLVGLGERMDHYPRQLSGGQEQRVGLARAI
                     VSDPTMILADEPTGNLDRESAEDILTLFGRLNRELGKTIVMVTHDPRAAERAQMIRHL
                     EKGVLESPP"
     gene            complement(3206..4480)
                     /locus_tag="PKK81_10465"
     CDS             complement(3206..4480)
                     /locus_tag="PKK81_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV33347.1"
                     /translation="MSAQPTIEQPEPRKPSIHDADLDGLAIRRTEPHLPRRNGRGRWW
                     LWLLLLTLVTGGAFVAWKSGLTGYGETVEVTPVVRMPAGGVSGLAATGYVVAQRQALI
                     ASKGTGRLEYLGVKVGDPVKEGQIIARLEHSDIDALLQQMVAKLNVARAQLGAAKPEL
                     EDATLHFERVKMLLEKSFITKAEYDIAGARVRRAAAAVRSAEASVMAAEAERKAAEVQ
                     LENTNIRAPFDGIVVKKLAELGEVVSPMTASVRSGGSVVTIVDPASVMVDAEVSEAMI
                     NRVQAGQSAEIQLDSVPGHRYRGEVVQVMPIADRAKATILTRVRFLELDERVRAELSA
                     KVTFGPKPGSVEEDRESWGVPPTAVVSRDGRQIVFVVKDAVVTERVVEAVSTSGPIQA
                     VHGLLTQADEVVVAPPADLRAGAPVTIRRRTL"
     gene            complement(4546..5763)
                     /locus_tag="PKK81_10470"
     CDS             complement(4546..5763)
                     /locus_tag="PKK81_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNV33348.1"
                     /translation="MAQEIAAPASLAGNQAGKTQARPVWKYVWRTWWVQVHLYLGLSV
                     GALLVVFGVTGSILVFFQEIDEWLNPVLLTVSVPSPGSAPVRPLQDIVTAAQQAATPG
                     SRIVQLYGPRNRTGVFAVYAEHPSKAWQRIYVDPYRAAVTGVRSYAAYEWVPHYLMDV
                     VFQLHFALFLGENGLIIVALGALLLLLSLISGVIVWWPLTGQWRQAFVLRWPSTPVRL
                     NFDLHKTFSLYTCLVLGAVLLSGVWMNWNDPFVWVVRLLSPATRGPKQAPVSIPLEGR
                     QPITPAQALAIATARYPEGVLNILVMPEDSTGIYLVGRREVPGLSLFWSERTVSIDQY
                     SGAILDVREPATRRSAGETFADWQWPLHSGQAFGLPGRLMVCLSGLACPVIYVTGVRM
                     WWRKRSARRMRPA"
     gene            complement(5946..6344)
                     /locus_tag="PKK81_10475"
     CDS             complement(5946..6344)
                     /locus_tag="PKK81_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33349.1"
                     /translation="MNDIFRLMSLRGRTGRTPSLLLLLACLLVAIRLAAGFVCVTCFT
                     DFEKPTSRSFHLHAGGDLNPCHHGRVEAGPLINWACTVTQDDTAFILPDVPLLPVVVS
                     LFVPLLLLVVSYGNRLLIAAHGRSPPPVIA"
BASE COUNT         1292 a         2191 c         1824 g         1038 t
ORIGIN      
        1 gcctgcacga tgacgagacg cgcggcgcgg gtggccggaa tgaggccgcc gacgatgctc
       61 atgagcatcg caaagacgag agcgatcgcg atcatctcgc ccgagaggtg aaagccgaag
      121 accagttcgg tcccggtgtc gaagttcatg gtggagatcg tcatcgactg gagtcccacc
      181 gcggcggcga cgcccagcaa gcccgcgatc gcgcccagcg ccatcgactc cagcagaaag
      241 gcctgcagga tatccatccg cgtgaacccg atggtccgca gcgtcccgat ctcggtcgtc
      301 cgctgcgcca ccgacgccga catcgtcatc gtcgctccga gcagcgcgcc gacactgaag
      361 accacggtca gaaacagccc cgtgacgcga atcattctag cgaggccctc cgccttgcct
      421 tcgtaatact ccggctcgcg cttcaccgag aatttaaacc gtgggtcccg ctcgatccgg
      481 tccttgaagg cgggcagcgt cgcaggctgc gccaaccgga gggtcaggga cgaaaaatta
      541 ctgcgatgaa acgtcgccag gaattgttcc gcatcgcccc agacctcgga gtcgaatccg
      601 ctgccgccgg cctcgaacac cccgaccacg gtccaggccc gattcccgaa gtgcaacacc
      661 cgatcgacac gggcgtcagg aaactgcctg gcgatggagg cacccacgac gatttcggtg
      721 gtacccggct tccacagccg cccctgaatc agcaggatcg ccggccgcac cgccagcgcc
      781 tctggtgccg tgccccgcaa ggtcacgctg gtcagactgc cgccctgccc cttccgcagc
      841 gtcaattgca gtccgacttc agcgaccgcc agcggccggc catccgccgt cagtcgaaca
      901 tccggctgag ctgccacagc gcgtatttcc tcgcgggaca ggctgctgcc gatttccgag
      961 agcgcgcctt tacgcagaat gatcacgttc tccggatcgc ccgtcttccc cagtgtctgc
     1021 tccaatccat gggccaacat caacacggcc agtaacacga acaccaccag ccccaaacca
     1081 acgacggtca acgccgtcgt cacacggcga gcccacagat ttctgaggct gtacagaccc
     1141 caccggaaca tgtccctacc tccgcctcgg ggcctgtctg agtaatgcca ttctgctgcg
     1201 gcaaatgatc tgccggcatc tgcgtcgttc tcggcccgta aaaatcctca acgtattcag
     1261 cgaatacgtt tccggttttt tcgagcctgc gtcctcgcat ccgccggcaa ctcctccgcc
     1321 tcgctacaaa ggattactct gacaggctcc tatcccgcat gccgcagccc gtccaacgtc
     1381 gtcatccgga tgagacggac cgccggccag atcgccgcgc ccaaggccac cagcaccatc
     1441 atgcccagac acaaccccac cgtctccatc gacaccgcgt aagtggccac atacccgttc
     1501 gattgggtca tctgcgcgta gagccgcgag gcggggacga gcaggcccaa tcccagggcc
     1561 ccgccggaga gcgccagcaa caacgactcg cccaacacca ggatgcaaaa atgccgtggc
     1621 tgaaatccca gcgtcttgat cacggcatat tccctggtcc gttcccggac cgccatggcc
     1681 agcgcgttca ccaggaccag gagcgcgatc ccgttgatga ccgcggacac cgcttccagg
     1741 ccgtacagca gtgcgctcga ccgctcgacc cacccggcaa tgaacgcctg ctccagttcc
     1801 gtgcgtgtct cggcaaacga gttcgcaaac agcgcatcga gcgccagggc caccgcccgt
     1861 ggattactcc cctctttcac atcgatcgcg taccacgaca cctgatcggc gcggtccggc
     1921 tgtgtcgtct tcacctgttc attgagatac tcccactgca gaaacaattc ctgatccgac
     1981 cagatcatga ggttgctgct gcggtaaatg ccacgcacaa tcacttccca ttgccccgga
     2041 tagctggtcc cggtcagcgg aatgacatcg ccgagtttcc atccgtaccg ctcggccagg
     2101 gacaggccga cgatgcaccc gcggcgatcc tgcacaaaac ccagacgctc cccctcggag
     2161 agcagaattt ccggatatgt ggtcagcaat gtcgccgccg gctcggcgaa ggaggccaac
     2221 ggctgcttgg gatccttgta gatcccgccg aactcgacgc catagtgcac ggaccgcacc
     2281 cccggcagca ccgccatgcg atctctgtat gccaacggaa gcggcaaggc ccccgacatc
     2341 gcatggaccg tgatcagccg gttcttgctg gccgcttttg ccgccccctg ccattcgtcg
     2401 agcgtcagac ggatgaggcc gaacgccagc acgaccatgg cgagccccca gaccgtcaag
     2461 gccagccgga ccgggcggcg caacgtattg cggagcacga gccggccgag cgtcatggcg
     2521 gagattccag gacgcccttt tccagatgac gaatcatctg cgcacgctcg gcggcgcgag
     2581 ggtcgtgcgt gaccatcacg atggtttttc ccagttcccg attcaatcga ccgaacaggg
     2641 tcagaatatc ctcggcggat tcacgatcga gattgcccgt cggctcgtcc gccaggatca
     2701 tggtggggtc gctgacgatg gcgcgggcaa gaccgacgcg ttgctcctgc ccacctgaaa
     2761 gctggcgcgg gtaatggtcc atccgctccc ccaggcccac cagccgcaag gccgtcttca
     2821 catgctccct ccgttcctgc cgcgacagat gtgtgagcag gagcggcagt tccacattct
     2881 ccgaggcggt gagcaccgga atgagattgt acgattggaa cacgtagccg atgtgccggg
     2941 cccgccatcg cgccatgtcg ccttcggaaa gatcgtcgag tcgggtgccg gccacgacga
     3001 ccgacccgct ggtcggccga tcgatccccg ccatcacatt gagcaacgta cttttcccgg
     3061 accccgaggg ccccatgata gcaaggaacg ttcccgcggg aatcgtgaag gagagatcgc
     3121 gcaacacgga cacgtccgtg cctgcccgcg aataggtttt cgagatccgt tggagcgtga
     3181 cgacgggatc gtacacggtg cagccttata acgtccgccg ccggatggtc accggggcac
     3241 cggcccgcag gtcggccggc ggcgccacga cgacctcatc ggcctgagtc aacaggccgt
     3301 gaacggcttg tatcggcccc gaggtcgaga cggcctccac cacgcgttcc gtcaccaccg
     3361 cgtctttgac gacgaatacg atctgacggc catcgcgact cacgacggcc gtcgggggaa
     3421 cgccccagga ttctctgtcc tcctcgaccg atccgggctt cggcccgaac gtcacttttg
     3481 cgctgagttc ggcgcggacc cgttcatcca attcgaggaa tcgcacccgc gtcagaatcg
     3541 tcgccttggc ccgatcggca atgggcatca cctggaccac ttcgccgcga taccggtgtc
     3601 ccggaacgga atccaactga atctcggcgg actgcccggc ctggacgcgg ttgatcatgg
     3661 cttccgacac ctcggcatcg accatcacgg acgcgggatc gacgatggtg acgacggagc
     3721 cgccggaacg gaccgacgcg gtcatcggag agacgacttc gcccagttcc gccagtttct
     3781 tcacgacaat cccgtcgaag ggcgcgcgga tgttcgtgtt ttcaagctga acctcggccg
     3841 ctttccgttc cgcttccgcc gccatcacgg acgcttcggc cgatcggacc gctgcggccg
     3901 cgcgccggac tcgcgccccg gcgatatcgt attccgcttt ggtgatgaac gatttctcca
     3961 acagcatctt cacccgttcg aagtggagtg tggcgtcttc cagttccggc ttcgccgcac
     4021 ccagctgcgc ccgcgccacg ttgagcttcg ccaccatctg ctgcaacagc gcatcgatat
     4081 ccgaatgttc gagccgcgca atgatctggc cttccttcac cgggtcgccg accttgaccc
     4141 cgagatactc cagccgaccc gtgcctttgg aagcgatcaa cgcctggcgc tgcgcgacga
     4201 cgtaccctgt ggcggcgagc cctgagacgc cgcctgccgg catgcgcacc accggggtca
     4261 cctcaaccgt ctcaccgtag ccggtgagac ctgacttcca ggccacaaac gcgccgcccg
     4321 tcaccagggt caggagcagg agccacagcc accaccggcc ccgcccgttg cgccggggca
     4381 gatgcggttc agttcgccgg atggccaacc cgtccaagtc cgcgtcatga atactgggct
     4441 tcctcggttc cggttgttct atcgtcggtt gtgcagacac aagacctcca ttttcaggcg
     4501 gctgacactc taccgcagag aaacgtcact ccgcacgcac ccccttcagg ccggacgcat
     4561 gcggcgtgcc gaccgctttc gccaccacat ccgcacgccg gtcacataga tgaccggaca
     4621 ggccaatccg ctcagacaga ccatcaggcg accgggtaat ccgaatgcct ggccggaatg
     4681 taacggccat tgccagtccg caaacgtctc cccggcgctt cgccgggtgg cgggctcacg
     4741 cacatccaga atggcgccgc tgtactgatc gatactcacc gttcgttcag accagaagag
     4801 gctgaggccc ggcacctccc tccggcctac cagatagatg cccgtcgaat cctccggcat
     4861 cacgagaata ttcagcactc cttccgggta acgagccgtc gcaatcgcca gcgcctgcgc
     4921 gggagtgatc ggttgccggc cttcgagcgg aatggacacc ggagcctgct taggcccacg
     4981 ggtcgcaggg gacaagagcc ggaccaccca cacgaacgga tcgttccagt tcatccagac
     5041 gcccgaaagc aagaccgcgc cgagcacgag acaggtgtag agcgaaaagg tcttgtgcag
     5101 gtcgaaattc agacggaccg gagtactggg ccatctcaaa acgaaggcct gccgccactg
     5161 gccggtgagc ggccaccaga caatcacccc gctgatcaac gacagcagca ggagcaatgc
     5221 gcccagggcc acgatgatca gcccgttttc acccagaaac agcgcaaaat gaagctgaaa
     5281 gacgacgtcc atcagatagt gcggcaccca ttcataggcg gcgtaactgc ggacgcccgt
     5341 cacagcggcc cggtaagggt cgacatagat tcgttgccag gctttcgacg gatgctcggc
     5401 atagaccgca aacacaccgg tacgattccg ggggccgtac aactgaacga tccggctccc
     5461 cggtgtcgcc gcctgctgag ctgcagtcac aatgtcctgc agcggacgaa cgggagcaga
     5521 ccccggcgac ggcacagaca ccgtcagcag gactggattc agccactcat cgatctcctg
     5581 gaaaaatacg aggatactgc cggtcacgcc gaacaccacc agcaaggccc caacggataa
     5641 tcccaagtag aggtgcacct gcacccacca cgtgcgccac acatatttcc agacaggtcg
     5701 cgcctgcgtc ttcccggcct ggtttcctgc cagggacgcc ggtgcggcga tctcttgcgc
     5761 cattgttact actccaacga atcgagccgg aatgcgccgc gaggttcctg acgccgccac
     5821 gcgtccacgc cctgccgacc ccacggggat ggagcctgag gacagtgaaa cgacccggca
     5881 caggaccgca tgaaatgcga cccgagccgg attggtgacc gttcagaagc gttgcgtaac
     5941 tggaatcagg cgatgacagg aggagggcta cggccgtggg ccgcaatcag cagtcggttt
     6001 ccataagaca ccacgaggag cagcagggga acaaacagcg agaccacgac cggcagcaga
     6061 ggaacatcgg gcaggatgaa cgccgtgtca tcctgcgtaa cggtgcaggc ccagttgatg
     6121 agggggccgg cctccacccg gccatggtgg cacggattca ggtctccccc ggcgtggaga
     6181 tgaaaactgc ggctggtcgg cttctcgaaa tcggtaaaac aggtcacaca cacaaagccg
     6241 gccgccaacc ggatggcaac caggagacag gcaagtaaga gcaggagcga cggagttcgt
     6301 cccgttctcc cacggaggga catgagccgg aagatgtcgt tcacc
//
LOCUS       DAOQRM010000141         6255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_12538_length_6255_cov_15.225161, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000141 DAOQRM010000000
VERSION     DAOQRM010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12538_length_6255_cov_15.225161"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="PKK81_10480"
     CDS             <1..1770
                     /locus_tag="PKK81_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV33350.1"
                     /translation="PECVKVLDLDGSVRTINAAGLAMIEATSSADIVGRDVCHLATAE
                     FRSVYRDLIGKAGRGEAGRLEFRMVGLRGTPRWLDTHMVPLRSADGIVTAVLGVTRDI
                     TEWKKSETLLRQSEERYRRLLAVLPDAILVNRENRIFFANEQGVRLFGARSAEEILGK
                     SLYDLAHPDYHEMIDARIRHLLGPGNTVPEVEEKIVRLDGMSVDVAVRAARFQDEEGF
                     GILVVLRDISLRKAAEQKLRESEERLQSLLGAMEDVIWSSSLDLSTMFYVSPSVMEIY
                     GRPPDAFLARPSLWLEAVHPDDCVIAEQARQALSTMGEFDVEYRIVGLDGDLRWLHDR
                     GRVIKDADGRPLRIDGIASDITERKRLQAQLRRTERVAELGTVASGMAHEIGTPMNVI
                     LGRAEYLMERTKEEPVRKGLQTIVSQVERITRVMNQLLAFARRRPVEHRALDLRQTIE
                     DNLEIFQERLSQSNISVETSFADACPLVHADSDQMSQVLINLVMNAIHAMPGGGVLKL
                     SLAPDRGMVRLTVADTGHGMPRDVIAKVFDPFFTTKEFGKGTGLGLTVVKGIIEEHSG
                     TIEVESEPERGTTFTICLPIDKS"
     gene            2061..2492
                     /locus_tag="PKK81_10485"
     CDS             2061..2492
                     /locus_tag="PKK81_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV33351.1"
                     /translation="MVTYSEGSNVESRTPAVLLVVEDDKDMRSLLCDELWGEGYQLRE
                     ASNGEEGLAAVMRAAPDLIVTDLKMPAGGFDYVHRLRSCVPGCPIIVMTAFGDAQTKD
                     EAMKNGATAYFDKPVRLSELKATVKQLLQHPGSRSEDGLLH"
     gene            2636..4039
                     /locus_tag="PKK81_10490"
     CDS             2636..4039
                     /locus_tag="PKK81_10490"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV33352.1"
                     /translation="MAGSHSQGTPFSPFTDPILAARLEALKLLADGLTDRVAVMDRDL
                     NVVYANDAAWAKSGNPSAVAKPAKCYQAFVHMTDPCGTCPAESVFKTGEVLTVSCNAS
                     GDGTACGMHQAFPLVSSAGEVASILVLFHKRPEPLSIPASLPQQRLGRSRPERTESRL
                     GDLVGASPAMNQLFEMIRLVADSSATVLIQGESGTGKELVARTIHQTSYRRDKPFVVV
                     DCGALPETLLESELFGHVKGAFTGAAGAKSGLFEEADGGTIFLDEIADTSPTFQAKLL
                     RVLQEGEIKRVGGTQPVKINVRVISATNKDLTDLVKAKAFRQDLYYRLAVLPVHLPPL
                     RDRRVDIPLLVVKFIADSCQRHRQDLRQVSGEAMRALTAAPWPGNVRELQHYIERAVV
                     TTTHTELTCADLVELGSHPQDADLRTVGRGAARQAERVRILQALQQTSGNRVQAARVL
                     KISRASLYNKLHELGIQ"
     gene            4614..>6255
                     /locus_tag="PKK81_10495"
     CDS             4614..>6255
                     /locus_tag="PKK81_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33353.1"
                     /translation="MRSSVQSKRRRISRKAMLSLQAALLSGSLLAAPWVFAAGPGDQS
                     HEGHATPVAMPGWTQTLKGQTVVENAMEGRAGNAEKMEMQHHRLMEKLEQQAQTDAKA
                     QQTSGAFNEMSMMHQYMGQDGSSFLLASDSSKGEPVMTSGGKCPANAPVKKYDVSMIN
                     IEISLNRWLDFYPGYMYVHTGEIDKVRAEETKNKEAREKDGFDPGAVTTGNQGDAIQP
                     LVLRANQGDCVKMTLRNQMEGEDGSLVIQASSMIVSATGKPATTTNPDTVVSPGKTQE
                     FEWYIHPNMQEGVRQFHSWSHDRELTVLGLFGAFIVEPKGSKYLDPLGTGAAPDTNSG
                     WQVMIDNGSGPDFREFVLFYHEIGDEAFRPLNKKGDFLPQRDPLTDAYRPGGRAINYR
                     SEPFGIDQMHLQHEYFGFEDESLAYSAYTFGDPPTTVARGYLGDPVKWRLVHGGSEVF
                     HSHHPHSGTIRWQRSPRADQEEHWFKGQDGPVKYPVVRTKSDRVDVEVVGPSEALDLE
                     PECGGGGCQHLAGEFLYHCHVAHHYVAGMWGYGRFYNTL"
BASE COUNT         1258 a         1669 c         2034 g         1294 t
ORIGIN      
        1 cccgaatgtg taaaggtgct tgatctggac ggcagcgttc gaaccatcaa cgccgcaggg
       61 ctggccatga tcgaagcaac gagcagtgca gacatcgtcg ggcgggatgt gtgccacctg
      121 gccacggccg agtttcggtc ggtctaccgt gacctgatcg gcaaggccgg gcggggggaa
      181 gcggggcgac tggagtttcg gatggtcggt ctccggggaa cgccgcggtg gctggatacg
      241 catatggtgc ctctgcgttc agccgacggc atcgtgacgg cggtcttggg agtgactcgc
      301 gacatcaccg agtggaagaa gtccgaaacg ctcttgcgcc aaagcgagga gcggtatcga
      361 cggcttttgg ccgtattgcc cgatgccatc ctggtcaatc gcgagaaccg gatctttttc
      421 gccaatgaac aaggggtgcg tttgttcggg gcccgatcgg ccgaggagat tctcggcaag
      481 tcgctgtatg acctggcgca tcccgactat cacgagatga tcgatgcgcg gatccggcat
      541 cttctggggc ccgggaacac ggtgcccgag gtcgaggaaa agatcgtgcg cttggacggg
      601 atgagtgtgg acgtggctgt cagggctgcg cggtttcagg atgaggaggg gttcggcatc
      661 ctggtcgtcc tgcgggacat ttcgcttcga aaagccgccg agcagaagtt gcgggagagc
      721 gaagagcggc ttcaaagttt attgggagcg atggaggatg tcatctggtc gtcgtcgttg
      781 gacctgtcca cgatgtttta tgtcagtccg tccgtgatgg agatctatgg ccggccgccc
      841 gacgcgtttt tagccaggcc ttcgttatgg ctggaggccg ttcatcccga cgattgcgtg
      901 attgcggaac aggcgcgaca ggccctctcg acaatggggg agttcgatgt ggagtatcgc
      961 atcgtcgggc tcgacggcga tctgcgttgg ctacacgacc gcgggcgggt catcaaggat
     1021 gccgacggcc gtccgttgcg catcgacggc attgccagcg atatcacgga gcggaagcgc
     1081 ctgcaggcgc aacttcgccg gacggagcgc gtggccgaac tgggaacggt ggcatccggc
     1141 atggcgcatg agatcggcac acccatgaac gtcattctgg gccgggccga atatctgatg
     1201 gagcgcacga aggaggagcc ggtcaggaag gggctccaaa ccatcgtcag tcaggtcgaa
     1261 cgaattacgc gggtgatgaa tcagttgctg gcgtttgccc ggcgacgtcc ggtcgaacac
     1321 cgtgccttgg acttgcgcca gaccatcgag gacaacctgg aaattttcca ggaacgcctg
     1381 tcgcaaagta acatcagcgt ggaaacctcc tttgccgacg cctgcccctt ggtgcatgcc
     1441 gattccgatc agatgagtca ggtcttgatc aacctggtga tgaatgcgat ccatgcgatg
     1501 cccgggggag gggttttgaa gctgtcactg gcgccggacc gtggcatggt ccggttgacg
     1561 gtggcggata ccggccacgg gatgccccgg gacgtgatcg ccaaggtgtt cgacccgttt
     1621 ttcaccacca aggagttcgg gaagggaacc gggttaggtt tgactgtggt gaagggcatc
     1681 attgaggaac acagcggaac gattgaggtg gagagtgagc ctgagcgggg gacgaccttc
     1741 accatttgcc tgccgataga caagagctga tagcgtattg catttagctt atggcaggga
     1801 ttgaacagtc tttgggaatg gtcggcctct tttcttcgct cgtgccatac gccatcggca
     1861 attcgccata agctccactc gtcccgttgt ggcgcttcgg gacagggctt tttcagaggg
     1921 tggggtcttt ctcgccagag gccggttcat gcacctactg tttggagaaa ccttcggggc
     1981 tagttatcca ttgaatacaa tcagttgctc gctttcacgc gccgacgtga gtggcacatg
     2041 gtttgcgttg tgttgtcccc atggtgacct attccgaggg ctcgaacgtg gaaagtcgaa
     2101 caccagcggt attactggtg gtggaagacg acaaggatat gcggagcttg ctctgcgacg
     2161 agttgtgggg agagggatac caattgcgcg aggccagcaa cggggaagag gggctggcgg
     2221 cggtcatgcg agcggctccc gatctgatcg tgaccgactt gaaaatgccg gccggcggtt
     2281 tcgactatgt ccatcgattg aggagctgtg tgcccggttg tccgattatc gtcatgaccg
     2341 cctttggcga tgcccagacc aaggacgagg cgatgaaaaa cggcgcgacg gcctacttcg
     2401 acaagccggt caggctgtcg gaactgaagg cgacggtcaa gcaactcctg caacatccag
     2461 gaagccggtc cgaggatgga ctgctgcact gaacattggc agtggatacg tgttttcgcg
     2521 aggtctcccc caagggggga gggttgtctt cgtgatcgtg gctgtagtac taatagggcc
     2581 ttcataaaaa tcagaaaggc cccgcttggt tcgtatagaa acgaggagtg atgagatggc
     2641 cggatctcac tcacagggaa cacccttttc cccctttacc gatccgattc tggccgctcg
     2701 gttggaagct ctgaagctct tggccgacgg gctgaccgac cgcgtggcgg tgatggatcg
     2761 ggatctcaat gtggtctatg ccaatgacgc cgcttgggcg aagtcgggga atccctctgc
     2821 ggtcgccaag ccggccaagt gttatcaggc tttcgttcac atgacagacc cttgcgggac
     2881 ttgtcctgct gagtcggtct ttaagacggg ggaagtgctc acggtctcct gcaacgcgtc
     2941 gggcgatggg actgcctgcg gtatgcacca ggcgtttccg ctcgtctcgt cggcaggaga
     3001 ggtggcctcg atcctcgtgc tctttcacaa gcggccggag ccgctcagta ttccagcgtc
     3061 gctgccgcag caaaggctcg gtcgatcgcg cccggaacgg accgaatcga gattgggcga
     3121 tctggtcggc gcgagtccgg ccatgaatca gctgtttgaa atgatccggt tggtggccga
     3181 cagttcggcc acggtgttga tccagggtga aagcggcacg ggaaaggaac tcgtggcccg
     3241 gaccattcac cagacgagct atcgtcgcga caagcctttt gtggtggtcg attgcggggc
     3301 gctcccggaa acgttgctgg agagtgaact gttcggccat gtcaaaggag cgtttaccgg
     3361 agcagccggg gccaagtccg ggctgtttga agaggccgac gggggcacga tctttctgga
     3421 tgagatagcc gatacgtctc caaccttcca ggctaagctt cttcgagtct tgcaagaagg
     3481 cgagatcaag cgagtcggcg ggacgcagcc ggtcaagatc aatgtccgtg tcatctccgc
     3541 caccaataag gatctgactg acctggtcaa ggccaaggca tttcgtcagg acctctacta
     3601 tcggttagct gtgttgcccg ttcatttgcc gccgctccgt gaccggaggg tggacattcc
     3661 cttgttggtc gtaaaattta ttgccgactc ctgccagcgg catcggcagg acctgcgcca
     3721 ggtgagtgga gaggccatgc gcgccttgac tgctgcgccc tggcccggga atgttcgcga
     3781 gctgcagcat tatatcgagc gagccgtggt gaccacgacc catacggagc tgacctgtgc
     3841 ggatctggtt gaactgggga gtcaccctca ggatgccgac ctcaggacgg tcggccgggg
     3901 cgcggcgcga caggccgagc gggtgcgtat ccttcaggca ttgcagcaga cctccgggaa
     3961 tagggtgcaa gcggctcgtg tccttaaaat cagccgggcc agtctctaca acaagctgca
     4021 cgaactcggc attcagtagc tcagcccaga gagcctgttt tcaggccctc cccgcttctt
     4081 gtctctatcc taatcttctt cagaatcagt ccaatggctc ttcctttcgt aaccgctaag
     4141 cgaggaagct gtcccgtatc gccccataat tcacgatcta atgtccagcg gcttggacag
     4201 tctaagtaat tgtattttat atgttcttat ccttgccgag gtgtgctgtc taatcgtctg
     4261 gaaaactgtg gcattttacg ccggttcaaa atttcccgcc taaatcttca actccttgaa
     4321 aaatatataa catttcagtc cggctaggac tacccctagt gggcacatca attgcgttgt
     4381 taggcagtgc tggtgaaggt gcgggtgatt aggaccggcc tccgaatgac ctgctccctc
     4441 gatctttgag gacatccttc accagccctt tttccttttg ggagcgtttc gtggagggaa
     4501 aaaggagagg ccggaggagc cggggtcctg cctgacggag gccggtcaca cgaccacgtt
     4561 ttttaaccac tcccgcggaa gggcgcggga ttataaagga gggaggccga catatgaggt
     4621 cttcagtgca gtcaaagcgc aggcgcatca gtcgtaaggc gatgctgagc ctgcaggcgg
     4681 cgctgctctc gggcagtttg ctcgcggcgc cgtgggtgtt tgcggctggt ccgggcgatc
     4741 agtcgcatga gggacatgcg acgccggtcg ccatgccggg ctggacacag accctgaagg
     4801 gtcagaccgt ggtggaaaac gccatggaag gtcgcgccgg caatgcagag aagatggaga
     4861 tgcagcacca ccgcctgatg gagaagttgg aacagcaggc gcagacggac gcgaaggcgc
     4921 agcagacctc cggtgcattc aacgaaatgt cgatgatgca ccagtatatg gggcaggacg
     4981 ggagcagctt cctgttggcg tccgattcgt cgaagggcga accggtgatg acgtcagggg
     5041 ggaaatgtcc cgccaatgcg ccggtgaaga agtacgacgt ttcgatgatc aacatcgaaa
     5101 tcagtttgaa ccggtggctc gacttctatc ccgggtatat gtacgtccat accggggaga
     5161 tcgacaaggt tcgcgccgag gaaaccaaga acaaagaagc gcgggaaaaa gacggattcg
     5221 atcctggtgc ggtgacgacc ggaaaccagg gcgatgcgat tcagccgctg gtgctccgtg
     5281 ccaaccaggg cgattgcgtc aagatgacgt tgcggaatca gatggagggt gaggatggca
     5341 gcttggtcat ccaggcctcc agcatgatcg tgagcgccac gggcaagcct gcgacgacga
     5401 cgaacccgga cacggtggtt tcgccgggca agacgcagga gttcgaatgg tacattcatc
     5461 cgaacatgca ggaaggtgtg cggcagttcc actcatggag ccatgatcgc gagttgacgg
     5521 tgttgggcct cttcggcgcc ttcatcgtcg aaccgaaggg ttccaagtac ctcgatccgc
     5581 tcggaacggg cgccgcgccc gatacgaaca gcggatggca ggtcatgatc gacaacgggt
     5641 ccggaccgga cttccgcgag ttcgtgttgt tctatcacga gatcggcgac gaagccttcc
     5701 ggccgctgaa caagaagggt gacttcttgc cgcagcgcga tccattgacc gacgcctatc
     5761 gtccgggtgg ccgtgcgatc aactatcgca gcgagccgtt cggcatcgat cagatgcact
     5821 tacagcatga atatttcggc ttcgaagatg agtcgctggc ctacagcgcc tacaccttcg
     5881 gtgacccgcc caccacggtg gcccgtggct acctcggtga tccggtcaag tggcgtttgg
     5941 tgcatggtgg atcggaagtg ttccactctc accatccgca cagcggaacc atccgttggc
     6001 agcgcagccc gcgcgccgat caggaagagc attggtttaa gggacaggac ggcccggtga
     6061 agtatcccgt ggtccggacg aagtccgatc gggtggacgt ggaagtggtc ggtccgtccg
     6121 aagcgctcga cctggaaccg gaatgcggcg gcggtggctg tcagcacctg gccggcgagt
     6181 tcctctatca ctgccacgtt gcgcaccact atgtggcggg catgtggggg tacggccggt
     6241 tctacaatac gttgc
//
LOCUS       DAOQRM010000142         6243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_12579_length_6243_cov_16.593568, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000142 DAOQRM010000000
VERSION     DAOQRM010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12579_length_6243_cov_16.593568"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PKK81_10500"
     CDS             <1..582
                     /locus_tag="PKK81_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HNV33354.1"
                     /translation="RKLTAAISKSQTTLIFINQIRMKIGVMFGNPETTTGGNALKFYS
                     SVRLDIRRIESIKDGQDVTGSRVRVKVVKNKMAPPFKQAEFDIMFAEGISKSGELVDI
                     GVEKRVVEKAGAWYSYKGERLGQGREAVRDFLKANPAIAKEIEGKVRDLAGLPSRGTE
                     KKVEGKEAKDEKPERKPEGRQDDKRGHSARVTT"
     gene            622..1080
                     /locus_tag="PKK81_10505"
     CDS             622..1080
                     /locus_tag="PKK81_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNV33355.1"
                     /translation="MKPTPDYLTLAIKYLARTDRTVAQVERYVLEKGASRAEERLVVR
                     ELERRGYLNDQAYAIRWAETRLSRHPMGRERLKAELLSRGFEDSVAERALRQAYRSIS
                     EQELACQALEGRASRTRPAQWVRFLRQRGFDDDTIQQVTQVDLETGLDEL"
     gene            1089..3707
                     /gene="alaS"
                     /locus_tag="PKK81_10510"
     CDS             1089..3707
                     /gene="alaS"
                     /locus_tag="PKK81_10510"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNV33356.1"
                     /translation="MNDLRRAFIQYFEQQGHRAVPSAPLIPQADPTLLFTNAGMNQFK
                     RVFLGEETRAYNRAVTVQKCLRAGGKHNDLENVGYTRRHHTFFEMLGNFSFGDYFKED
                     AIRFGWEFLTSVVGLSKDRMWITIFREDDEADRLWRKIGVSPSRIVRCGEKDNFWQMA
                     DTGPCGPCSELHFDQGPSVPGDATPNGEGDRVIEIWNLVFMQFNRDSAGTLNPLPKPS
                     IDTGMGLERLTAVAQGRLSNYDSDLFAPLLAAIGSRAGAQYGVLEQADRSMRVIADHL
                     RAITFLMADGVLPSNEGRGYVLRRILRRAARHGRLLGITEPFLHELTATVVEQMGEAY
                     HELRPAAGTVAEATRGEEERFIATLDQGLPILNDMLTKVRASGQNMLSGTEIFKLYDT
                     YGFPMDLIAEACREQDIRLDETGFEAAIEEQRTRARKTGGFEGETARPALSEVAARVG
                     TTAFVGYEHLNSEGVVQALLKGDRLVKDAREGDDIEVVLDVTPFYAEGGGQAGDQGLL
                     TGPDGRVEIRETTRPVPTLIVHKGVVTSGSIREGERLQLSVNRRTRHDAARNHTATHL
                     VHAALRDLLGPHVKQYGSLVAPNRLRFDFAHFRPLASRDIDEIESIVNEQVRQDQPVQ
                     TDVMGVQEAVAGGALAFFGDKYGDQVRVVHIDTFSKELCGGTHCRRTGEIGLFRIVSE
                     SGVAAGVRRIECLTGSGALDSLKRLEADVRELSDLLKVAPGEVVARTRKLNEQLKEKE
                     RELAEVKLKMASTSSGDAQAREIKGVQVHAQRTDGLDVNGMRALADQLRDKLRSGVVA
                     LGAANDGKVSLLVVVTKDLVGRLKAGELIKEMATEVGGTGGGRPEMAQAGGKNPEGLG
                     TALEKVFGLVQKALEG"
     gene            3712..4323
                     /gene="ruvX"
                     /locus_tag="PKK81_10515"
     CDS             3712..4323
                     /gene="ruvX"
                     /locus_tag="PKK81_10515"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNV33357.1"
                     /translation="MKGRRILAIDHGSKRIGFALSDELGWTAQPLETFYRRNPDADIR
                     HIQDLVREHEVGQVLVGMPLRLDGEIGPAAKVVAEFIQLLEPALSVPVITWDERMTTC
                     AAEDLLIAADVGRRKRKGIVDRIAAAILLQSYLASLEQPATSQANDLNAPVETDPWVF
                     DEPRVDDAEESDHGSGPGGSDVRGSRGISGAALGSKSRRERTS"
     gene            4229..5260
                     /gene="mltG"
                     /locus_tag="PKK81_10520"
     CDS             4229..5260
                     /gene="mltG"
                     /locus_tag="PKK81_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNV33358.1"
                     /translation="MGLALAAVMFAALAGYLVLRWAQSPVASGPPKPPSHIVLIPEGS
                     TFQQVAALLKNQQLIRSRSAFLLLGKTRDIDRKIRPGEYELDASMSPQDILTKLLAGR
                     VVLHPVTIPEGYSLTQIAEVLAAQQVTDTKEFTKLVRDRTFISTLGIEADSLEGYLFP
                     ETYSFAKGTKAREVIKAMVDGLHRVWGTELQEQAARMKMSLHQVLTLASVIEKETGAK
                     DERELIAAVFHNRLRKKIPLQSDPTVIYGLPAFDGNIHKRDLSVMSPYNTYRVQGLPP
                     GPIASPGAHSLRAALFPAQASYLYFVSRNDGTHQFSSTLAEHNQAVEKYQKQYFRKRA
                     RGNLVAHGA"
     gene            5335..6024
                     /gene="deoC"
                     /locus_tag="PKK81_10525"
     CDS             5335..6024
                     /gene="deoC"
                     /locus_tag="PKK81_10525"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNV33359.1"
                     /translation="MSVKSWNESLPRYLDHTVLRPEATKADVLRLCAEAREQGFVVIF
                     VPPCYVDEAVAAVAGTDVQVGIPIGFPLGGHSTHAKVTEAMEAVAHGARVLDMVINIS
                     RLKSGDYDVVRNDMAAVVQATPGVNHKVILETCLLTREEKITACRLAVEAGMDYVKTS
                     TGFNQAGATVEDVRLMKEAVAGRAKVKASGGIRDWKTTRELLEAGADRIGTSASLKIL
                     SEWRAALVAVR"
BASE COUNT         1282 a         1666 c         2086 g         1209 t
ORIGIN      
        1 cgtaaactga cggcagcgat ttccaagtcg cagaccaccc tgattttcat caaccagatc
       61 cgtatgaaga tcggcgtgat gttcggcaat cccgaaacca cgaccggcgg caacgcgctg
      121 aagttttatt cctcggtgcg tctcgacatc cgccgcattg aatcgatcaa ggacggtcag
      181 gatgtgaccg gcagccgcgt gcgggtcaag gtggtcaaga acaagatggc gccgccgttt
      241 aagcaggcgg agttcgacat catgtttgcc gaaggcattt cgaaatccgg cgagctcgtc
      301 gatatcggcg tggagaagcg cgtggtggag aaagccgggg cctggtactc ctacaaaggt
      361 gaacgattgg ggcaggggcg tgaagccgtc cgcgattttc tcaaggcgaa tcccgccatt
      421 gcgaaagaga tcgagggcaa ggtgcgcgat ctcgccggtc tgccttcccg cggcactgaa
      481 aagaaggtcg aggggaaaga agccaaggac gagaagcccg aacggaagcc agagggacgc
      541 caggacgata aacgcggtca cagtgccaga gtcacgacct aggtggtggc agtgcggaca
      601 gcgcgcagca cagagcggcg tatgaaaccg acgccggatt atctcacgct ggcgatcaag
      661 tatctcgcgc ggaccgatcg gaccgtggcc caggtcgagc ggtatgttct ggaaaaaggt
      721 gcgagccggg cggaagagcg cctcgtcgtt cgtgaactgg agcgcagagg ttatctgaac
      781 gatcaggcct atgcgatccg atgggcggaa acgagactgt cgcgacatcc gatgggccgt
      841 gaacggctca aggcggaact gctcagccgt ggctttgagg acagtgtcgc agagcgggcg
      901 ttgcggcagg cataccgttc gatttccgaa caggagttag cctgccaggc gctcgaaggg
      961 cgtgcgagcc ggacgcgtcc ggctcaatgg gtgcggtttc tgcggcaacg cgggttcgat
     1021 gacgacacga ttcagcaagt cactcaagta gatttggaga cggggttgga cgagttatga
     1081 gccaaagtgt gaacgatctg cgacgagcct tcatccagta ctttgaacag cagggacatc
     1141 gggcggttcc gagcgctccc ttgattcccc aggcagaccc gacgctgctg tttacgaacg
     1201 ccggcatgaa tcaattcaag cgggtgttcc tgggcgagga gacgcgtgcg tataaccggg
     1261 cggtgaccgt gcagaagtgc ctgcgcgccg gcggcaagca caacgatctc gaaaacgtgg
     1321 ggtacacgag gcgtcaccac acctttttcg aaatgctcgg caacttctcc ttcggcgact
     1381 atttcaagga agacgccatc cggttcggct gggagtttct gacctcagtc gtcgggctct
     1441 cgaaagaccg gatgtggatc acgatcttcc gcgaggacga tgaagccgac cgtctctgga
     1501 gaaagatcgg cgtctccccg agtcggattg tacgttgcgg cgagaaggat aatttctggc
     1561 agatggcgga tacgggccct tgcggtccct gctctgaact ccatttcgat caaggccctt
     1621 cggtgccggg cgacgcgacg ccgaacggag aaggcgaccg ggtcatcgag atctggaacc
     1681 tggtgtttat gcagtttaat cgcgacagtg cgggcaccct gaatccgctg ccgaaaccga
     1741 gcatcgatac cggcatgggc ctggaacggt tgacggcggt ggcgcaaggg cggctcagca
     1801 actacgacag cgatttattt gcgcccttgt tggcggcgat cggaagcagg gccggagcgc
     1861 agtacggtgt gctggaacaa gcggatcgtt ccatgcgcgt cattgcggat cacttgcggg
     1921 ccatcacgtt cctcatggcc gacggcgtct tgccgtcgaa tgaaggacgt ggatatgtgc
     1981 tccgccggat tctgcgacgc gcggctcgcc atggccggtt gctgggtatt accgaaccgt
     2041 ttctgcatga actgacggcg acggtcgtgg agcaaatggg cgaggcctat cacgaattgc
     2101 gtccggcagc cggtacggtg gcggaggcga cacggggcga agaagaacgg tttatcgcca
     2161 ccctcgacca ggggttgccg attctcaacg acatgctgac caaggtgcga gcctccggtc
     2221 aaaacatgct ctcgggtacc gaaattttta agttgtatga cacctatggc tttccgatgg
     2281 acctcattgc ggaagcctgc cgtgagcagg acatccgact cgatgagacg ggatttgaag
     2341 cggccatcga agagcagcgc acgcgcgcca ggaaaacggg gggcttcgag ggtgagacgg
     2401 cccgccccgc attgagtgaa gtggccgctc gtgtcggcac tacggcattc gtcggctacg
     2461 agcacctgaa ttcggaaggt gtcgtgcagg cgttgctcaa gggcgatcgg ctggtgaagg
     2521 acgcgcgtga aggtgatgac atcgaagtcg tgctggatgt cacgccgttc tatgcggaag
     2581 gcggcgggca ggccggcgat caaggactcc tgaccggtcc cgatggccgt gtggagattc
     2641 gtgaaaccac caggccggtg ccgaccttga tcgtgcacaa gggcgtagtc acatccggtt
     2701 cgatccgtga gggggagcgg ttgcagctct ccgtgaatcg tcgcacccgg cacgatgcgg
     2761 cccgcaatca tacggcgacg catttggtgc atgccgccct gcgggatctg ctggggccgc
     2821 atgtgaaaca gtatggttcg ctggtggcgc ccaatcggct gcggtttgac tttgcgcatt
     2881 tccgcccatt ggcctcacgc gacatcgacg agatcgagtc gatcgtcaat gagcaggtgc
     2941 gtcaggatca gccggtgcaa acggatgtga tgggggtgca ggaggccgtg gccggcgggg
     3001 cgctggcgtt tttcggcgac aagtatggcg atcaggtgcg agtggttcat atcgacacct
     3061 tcagcaagga attgtgcggc ggtacgcatt gccggcgcac gggcgaaatc ggcctgttcc
     3121 ggatcgtgtc tgaatcggga gtggccgccg gtgtgcgccg catcgaatgt ctcaccggta
     3181 gcggcgcgtt ggattcgctc aagcgattgg aagcggatgt tcgtgagttg tccgacctcc
     3241 tgaaagtggc gccgggtgaa gtcgtcgccc gtacccgcaa actgaacgag caactgaaag
     3301 agaaggaacg ggaactcgcg gaagtgaagc tcaagatggc gagtacctcc tcaggcgacg
     3361 cgcaagctcg cgagatcaaa ggggtgcagg tccacgcgca acgcacggac ggcctcgatg
     3421 tgaatggcat gcgggcgctg gccgatcaat tgcgcgataa actgcgcagc ggcgtcgtgg
     3481 cgctcggggc ggcgaatgac ggcaaagtgt cgctgctcgt cgtggtaacg aaggatctgg
     3541 tgggccgtct gaaagcgggc gaactcatta aggagatggc aacggaagtg ggcgggaccg
     3601 ggggaggacg tcctgagatg gcgcaggccg gaggaaaaaa tcccgaggga ctcggcaccg
     3661 cgttagaaaa agtttttggg ttggtccaga aggccctcga agggtagact gatgaaaggc
     3721 cgacggattc tcgccatcga tcacggctcc aagcggatcg ggtttgccct gagtgatgag
     3781 ctcgggtgga cggcccagcc gctggaaacg ttttaccggc gaaatcccga tgccgatatc
     3841 cggcatatcc aggacctggt tcgtgaacac gaagtcgggc aggttctggt cggcatgccg
     3901 ttgcggttgg atggcgagat cggtcccgcg gccaaagtcg tcgcggagtt catccagtta
     3961 ctggagccgg cactgtctgt cccggtgatc acctgggatg aacggatgac cacctgcgcg
     4021 gcggaggacc tgctgattgc ggctgacgtc ggccgccgga agcggaaggg gatcgtcgac
     4081 cgtattgccg cggcgattct cctgcagagt tatctggcga gtttggaaca gccggccacc
     4141 agccaggcga acgatctgaa cgcacccgtg gagaccgatc cctgggtctt tgacgaaccc
     4201 cgagtcgatg atgcagaaga aagcgatcat gggtctggcc ctggcggcag tgatgttcgc
     4261 ggctctcgcg ggatatctgg tgctgcgttg ggctcaaagt cccgtcgcga gcggacctcc
     4321 taaacctccc tcccacattg tcctcattcc tgaaggcagc acctttcagc aggtcgccgc
     4381 tcttctcaaa aaccaacagt tgatccgcag caggtcggct tttttattgc tgggcaagac
     4441 gcgggacatc gatcgaaaga tccgccccgg tgagtatgag ttggatgcca gtatgtcgcc
     4501 gcaggacatt ctgacgaagc tgctggccgg tcgggtggtg ctgcatcccg tcacgatccc
     4561 ggaaggatac agcctgacgc agattgccga agtgctggcg gcgcagcagg tgacggatac
     4621 gaaagagttc acgaagctgg tgcgagatcg cacgtttatt tcgaccctcg gcatcgaggc
     4681 cgattccctc gaagggtatc tctttcccga aacctattcc tttgcgaagg gtacgaaggc
     4741 gcgggaggtg atcaaggcga tggtcgatgg tctccatcgt gtctggggta ccgagttgca
     4801 ggagcaggcg gcccgcatga aaatgtccct gcatcaggtg ctgaccctcg cctctgtcat
     4861 cgaaaaggaa accggagcga aggatgagcg ggaactgatc gcggcggtgt ttcataaccg
     4921 gttgcggaaa aagatcccgc tgcagagcga cccgacggtg atctacgggt taccggcgtt
     4981 cgacgggaac attcacaagc gcgatctctc cgtcatgagc ccctacaata cctatcgtgt
     5041 gcagggtctc ccgccgggac cgatcgccag ccccggggcc cattcattgc gtgcggcgct
     5101 gtttcccgcc caggcgtcgt atttgtattt cgtgtcgcgg aacgacggga cccatcaatt
     5161 ttcttccacg ctggccgagc acaatcaagc ggtggagaaa tatcagaaac agtacttccg
     5221 caaacgtgcc aggggcaacc ttgtcgccca cggtgcgtga tcgtattgag aaccggtcac
     5281 gacacgtcct gtctcatttc ctcatcgatc gtagaatgtg aaagggacat tcgtatgagt
     5341 gtgaagtcgt ggaatgaatc gttgccgcgt tacctggacc atacggtgct gcgtccggag
     5401 gctactaagg ccgatgtgtt acgcctctgc gcggaagcca gggagcaggg gttcgtcgtg
     5461 atttttgtgc ctccctgtta tgtggatgag gcggttgccg cggttgcagg gaccgatgtc
     5521 caggtgggca tccccatcgg gtttccgctc ggtgggcatt cgactcacgc taaggtaacg
     5581 gaggccatgg aggcggtggc gcatggcgcg cgggtgctcg atatggtgat caacatcagc
     5641 cgtttgaagt ccggcgatta cgatgtggtg cgaaacgata tggccgccgt cgttcaagcg
     5701 acgccgggcg tgaatcataa ggtgattctg gaaacttgcc tgctgacgag agaagaaaag
     5761 atcactgcct gccgtctggc cgtcgaggcc gggatggact atgtgaagac ctcgaccgga
     5821 ttcaatcagg ccggagcgac agtggaagac gttcggctga tgaaagaagc cgtggccgga
     5881 cgggccaagg tgaaggcgtc cggtggaatc agagattgga agaccacgcg ggaattgctg
     5941 gaggcagggg ccgaccggat cggcactagc gcgagtctga agattctgag tgaatggcgc
     6001 gccgcgctgg ttgcggtgcg atgaaccgcc atccgtgtga aagccttgct cgttccggtg
     6061 gatttggtat agtgcgccca tgatgactcg aatcgtattg ctggtgctcg atggtttcgg
     6121 gatcggtgct ttgcccgatg ccgacctgta tggcgacgcc ggctgcaaca ccctgcagcg
     6181 gctggctgcc atttcgaagg ggctcgccct gccgaatttt gagcaactcg gtctcgggca
     6241 tct
//
LOCUS       DAOQRM010000143         6235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_12603_length_6235_cov_14.377184, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000143 DAOQRM010000000
VERSION     DAOQRM010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12603_length_6235_cov_14.377184"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /gene="proC"
                     /locus_tag="PKK81_10530"
     CDS             <1..519
                     /gene="proC"
                     /locus_tag="PKK81_10530"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNV33360.1"
                     /translation="AAGVTTAWIRERIVTPRGIVRAMPNTPALVREGVTALTCQSDLR
                     SDDLAAVRTLFEAVGSAVSIEERLMDAVTGLSGSGPAYVFVAIEALADGGVRMGLPRA
                     TAELLAAQTVLGAAKMVLERGEHPAKLKDQVASPGGTTIAGLYQLEAGGLRSCLMAAV
                     EAATKRSQELGR"
     gene            519..815
                     /locus_tag="PKK81_10535"
     CDS             519..815
                     /locus_tag="PKK81_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNV33361.1"
                     /translation="MFVLSNVLQGTATVLDTVLWLYMWVIIARALISWVNPDPWNPIV
                     QFLERATEPVLTPIRRLVGWRMGMDLSPMIAILILVFLQYAVVQSLRDIAVRMH"
     gene            837..1349
                     /locus_tag="PKK81_10540"
     CDS             837..1349
                     /locus_tag="PKK81_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNV33362.1"
                     /translation="MKITPIDIQQMVFQVKFRGYDRDEVNRFLEELALTVENLNRENS
                     ALREKLTATEQQVTDLRRTETTLSNTLVSAQTLAEDVKRSAQRESDLIVKEAELKASE
                     IIRQARVSLSDMQRGLADLQKQRLMMVERFRSTLRSFERMLEVEESDAYQSDAASVEG
                     KLTGESSTAR"
     gene            1464..2591
                     /locus_tag="PKK81_10545"
     CDS             1464..2591
                     /locus_tag="PKK81_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNV33363.1"
                     /translation="MATTNPLQAALQAAVDDGAFPGAVLAVRLRGTLIYEGAVGRLSP
                     QIPGEGVTIHTCYDLASLTKVLATTTALLLLMQRGKLTLDDRIDRILIALQGSAAGAA
                     SIHQLLTHSSGLPGWRPYYERLASLEAGQSGFPGRTAAREAVLGYIAQEALVYERGSR
                     SLYSDLGFMLLGWAVERLAGESLDQFCNNQIYRPLEAQPLAYVPRESQAMPAASLESH
                     AIAPTEDDPWRGRMLCGEVHDENAFALGGVAGHAGLFGTARAVLAVAKAWMDGRRGKT
                     GLLQPDLVTLFTTRQQGIPNASWALGWDTPSAPSSSGTRFTPESFGHLGYTGTSLWID
                     PVKELEVVLLSNRVHPTRKNERIKTFRPLIHDLICREFLGD"
     gene            complement(2588..2908)
                     /locus_tag="PKK81_10550"
     CDS             complement(2588..2908)
                     /locus_tag="PKK81_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33364.1"
                     /translation="MSGLPLLFKKEGLIERHQVEGIDPSDRYFNRAVLVSRVAAGYTG
                     KVTYEAYAVEGSAHSTTGAAVKAVVEKLMGVGFTRLRTRLNFKGNRYLAEKETWTDYP
                     DLPA"
     gene            3085..3621
                     /locus_tag="PKK81_10555"
     CDS             3085..3621
                     /locus_tag="PKK81_10555"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNV33365.1"
                     /translation="MNVPNSLTMLRILLIPVYVGLLNYEQFDYALATLFIAGLTDALD
                     GIIARVADQRTRLGEVLDPLADKLMLTTGFITLSVMHLVPLWLTILVVSRDLMLMLGA
                     AVAHFTHTQVDISPTVLGKGTTLVQLATLVAIIFFASRRLDLATLDPLLYLMGGVTLM
                     SGLHYLSRGYCRITSSQV"
     gene            3782..4999
                     /locus_tag="PKK81_10560"
     CDS             3782..4999
                     /locus_tag="PKK81_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV33366.1"
                     /translation="MSTFAYVGRNRQGAVKKGELTAKTRDEAVDQLRKQQVVVTSLEE
                     KSGMGGKFKFSLGSGLTDKDLVVFTRQFGTMINAGLPLIQCLDILSTQSENKVLRETV
                     GDVKNSVEAGSTFSDALKRHPKVFDDLYVNMIHAGEVGGLLDTILTRLAKHIEKAMKL
                     KGQIKSAMVYPTAIVGVAVIIISVLMVWVIPVFAQMFLEMSGGKVGLPGPTQIVINVS
                     NFFQSYWYAMGGAMVAAAIAIKRYYATVNGRVVIDRLLLKVPIVGDLIRKASVAKFTR
                     TLGTLITSGVPLLEGLSICAKTSGNKVIEEALMNARVSISGGKTISEPLAKCNVFPKM
                     VTHMIAVGESTGALDAMLGKIADFYEDEVDQAVETLTSLLEPIMMVVLGTIIGFIVIA
                     MYLPIFTMAQAIQ"
     gene            5206..>6235
                     /locus_tag="PKK81_10565"
     CDS             5206..>6235
                     /locus_tag="PKK81_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV33367.1"
                     /translation="MPELKTRLHWLMGLRVVVVTLMLGLSLAFQSTRGELVPTFSALI
                     ILTYATTIVYALLLRRLSAPAALTAFTWVQVGLDFVLETVLVARTGGIESPFAVLYVI
                     TVTVASLVPHRRVGLVTGAGCTLLFGLVTAVQYFGLLHASGWLPASRLEGAETLQTFG
                     VYGLAFLVVGFLSGVLADQLRHADQSLREKEQGLNRLQVFHESIVRSISSGVFTTDEE
                     GRITSFNPAAHEVTGYAFADVQGRLWRDVFNWHPGTPSEEAALTSLSPLRFEVECSHA
                     DGSRLVLGMTVSPLHEQGTQRGLVCVFKDLTQLRYLEQEMRRREWLANLGEMSAGMAH
                     EIRNPLGAL"
BASE COUNT         1159 a         1734 c         1953 g         1389 t
ORIGIN      
        1 gccgccggtg tgacgaccgc ctggattcgc gagcggatcg tgacgcccag aggaatcgtc
       61 cgggcgatgc ccaacacgcc ggctttggtg cgggaaggag tcacggcatt gacctgtcag
      121 tccgacctcc gatctgacga tctggctgcg gtacggacat tgtttgaggc ggtcgggtcg
      181 gccgtgtcga tcgaggaacg actcatggat gcggtgaccg gcctgagcgg aagcggccct
      241 gcgtatgtat tcgtcgccat tgaagcattg gccgacggcg gcgtccggat gggattgccg
      301 cgtgcgaccg ccgaactcct ggccgcgcag acggttttag gtgccgcaaa aatggttttg
      361 gagcggggtg aacatccggc caaattgaag gatcaggtgg cgtcgccggg aggcaccacg
      421 attgccgggt tgtaccagtt ggaggccgga gggctgcgga gctgtctgat ggcggccgtc
      481 gaggcggcca cgaaacgttc acaggagttg ggacgctgat gtttgtcttg agcaatgtac
      541 tgcaagggac tgccacggtg ctggatacgg tgttgtggct gtacatgtgg gtcatcatcg
      601 cccgcgcgtt gatttcgtgg gtcaatccgg atccgtggaa cccgatcgtg cagtttttgg
      661 agcgcgcgac ggagcccgtg ctcacgccga tccgtcggct ggtaggatgg cgcatgggca
      721 tggatctgtc gccgatgatc gcgattctga ttcttgtctt tttgcaatat gccgtggttc
      781 aatcgttacg ggatatcgcc gtgcggatgc attaaccgtc ggggaagagg tgtcgtatga
      841 aaatcactcc catcgacatt cagcagatgg tctttcaggt caagttccgg ggttatgacc
      901 gggacgaagt gaaccggttc ctcgaggagc tggcattgac cgtcgagaac ttgaatcgag
      961 agaacagtgc gcttcgtgaa aagctgaccg caaccgagca gcaggtcacc gacttgcgac
     1021 gcacggagac gacgctctcg aataccttgg tctcagccca gacgttggcc gaggatgtga
     1081 aacggtctgc gcagcgcgag tccgacctga ttgtgaagga agcggagctg aaggcgagtg
     1141 agatcatccg gcaggcgcgg gtgagtctct ccgacatgca acgtggtctc gcggatctcc
     1201 agaaacagcg tctgatgatg gtggagcgat tccgttcaac cctgcgatcg tttgaacgta
     1261 tgttggaagt ggaagaaagc gacgcgtatc agtcggatgc ggcttcggtc gaagggaagt
     1321 taaccggcga atcaagcacg gcgcgttgag cattctgcac tcccttgggc gtccatctcc
     1381 gcctgcgagc gggggctacc aagggggctc cgcccttccg ttctccctcc atctctctgt
     1441 gcatcacacg taggcgcgca ttcatggcca caacaaatcc ccttcaggct gccctacagg
     1501 cagccgtcga cgacggcgcc tttcccggcg cggtgttggc tgtccgattg cgaggaacgt
     1561 tgatctatga aggtgctgtc ggccgcctgt ccccgcagat tccgggcgag ggggtgacga
     1621 tccacacctg ctacgacctg gcctctttga ccaaggtgct ggcgaccacc acggcgttgc
     1681 tgttgctgat gcagcgcggc aagctgacgc tggatgatcg gatcgatcgc atcttgattg
     1741 cgctgcaggg cagtgcagcg ggtgcggcct ctattcatca attgctcacg catagctcag
     1801 ggcttcccgg gtggcgacct tattacgagc ggctggcttc gcttgaggca ggacagtccg
     1861 gatttccggg acgtacggcg gcgcgcgagg cagtgctggg gtatatcgca caagaagcgt
     1921 tggtctatga acgaggctct cgcagtctgt atagcgacct ggggttcatg ttgctgggat
     1981 gggcggtgga gcggctggca ggtgagtcgc tggaccaatt ttgcaacaac cagatctatc
     2041 gcccgctgga agcacagccg cttgcgtacg tcccgcgcga gtcgcaggcc atgcctgccg
     2101 cctcattgga atcacatgcg atcgctccga ctgaagacga tccttggagg ggacgcatgt
     2161 tgtgcggcga agtgcacgac gagaatgcct ttgctctggg cggtgtggcc ggccatgccg
     2221 ggttgttcgg cacggcgcgc gcggtactcg cggtggcgaa ggcgtggatg gatggccggc
     2281 gcgggaagac gggattgcta cagcctgacc tcgtcaccct gtttacgacc cgtcagcagg
     2341 gaataccgaa tgccagctgg gctttggggt gggatacgcc gtcagccccg tcttcttccg
     2401 gaacgcgctt cacgcccgaa tcattcgggc atctcggcta taccgggact tcgctatgga
     2461 ttgatccggt taaggaattg gaggtagtgc tgctgtccaa ccgggtacat ccgacgagga
     2521 aaaatgaacg gatcaagaca ttccgtccgc tcattcacga tctgatttgc cgggaatttc
     2581 tgggagatta ggctggcagg tccggatagt cggtccaggt ttctttttcg gccaggtagc
     2641 ggttgccctt gaagttcagg cgagtgcgca atcgggtaaa cccgactccc atcaacttct
     2701 cgacgacggc cttgacggca gcacccgtgg tggagtgagc ggagccttcg acagcatagg
     2761 cctcataggt gactttcccc gtatagcccg ccgccacacg actgacgagc accgctcgat
     2821 taaaatatcg gtcgctcggg tcgatcccct cgacttgatg ccgttcgatt aagccctctt
     2881 tcttaaagag gagggggagt ccactcatac aaacctccgt ctatcatctg ctgggagcgt
     2941 cttacacgtc tcgattatag ggacctccct tccggagtgc aaccgtcgtt gacaaggttc
     3001 tccaggggtt tctatgatgc gcgattcgcg tgacatcgtt cgccagtcga ggggccgatt
     3061 caaacaccag ctgtttcgat gaacatgaac gttcccaaca gtctgacgat gctgcgcatc
     3121 ctgttgatcc cggtgtatgt ggggttgctc aactatgagc aattcgacta tgcgctggcg
     3181 acgctcttca ttgccggatt gacggatgcg cttgatggaa tcatcgcgcg tgtggccgat
     3241 cagcggacca gactcggaga agtcctggat cccctggcgg ataaactcat gctgaccacg
     3301 gggtttatta cgctctccgt gatgcatctc gtcccgcttt ggctcacgat tctggtggtc
     3361 agccgtgatt tgatgttgat gctgggggcg gccgttgccc atttcaccca tacccaggtc
     3421 gatatttcgc cgactgtact ggggaagggc accaccttgg ttcagctcgc gacgctggtg
     3481 gccattatct tttttgcctc tcgccgtctt gatcttgcca cgcttgaccc gctcctgtat
     3541 ctcatgggcg gggtgaccct gatgtccggc ctgcactatc tctcccgcgg ctattgccgc
     3601 atcacgtcca gccaggtata gcgcgccgcc gctttgggtg ccgcttcttt catgttcttc
     3661 tgcgggtccg ctttcgtgca ttcgtttgac agtttttagg tcgtcttata gacttcgatc
     3721 atagttttcc gctgcatccg tacgtatcct gcctggtgtt caaaggagtg gaaaccgcgt
     3781 tatgagtaca ttcgcctatg tcggacggaa ccgccagggt gctgtgaaga agggggagct
     3841 caccgccaag acccgagatg aggcggtgga ccagcttcgc aagcagcagg tcgtcgtgac
     3901 cagcctggaa gaaaagtcgg gcatgggcgg aaagttcaag tttagcctgg ggagcggcct
     3961 gaccgacaag gatctcgtgg tcttcacccg tcaattcggc acaatgatca atgccggatt
     4021 gcccctgatc caatgtctcg atattctctc cacccagtcc gaaaataaag tgctgcgaga
     4081 aacggtcggc gatgtgaaaa acagcgtgga agcaggatcc acattctccg atgcgttgaa
     4141 gcgacacccg aaagtgttcg acgacctcta tgtgaatatg atccatgccg gtgaggtcgg
     4201 cggtctgctc gatacgattc tgactcgtct ggccaagcac atcgaaaagg cgatgaagct
     4261 gaaggggcag atcaagtcgg ccatggtcta tcccaccgcg atcgtcgggg tggccgtcat
     4321 catcatcagt gtcttgatgg tgtgggtcat tcccgtgttt gcccagatgt ttctggaaat
     4381 gtcgggcgga aaagtcggat tgcctggtcc gacccagatc gtgatcaacg tgagtaattt
     4441 tttccagagc tactggtatg ccatgggcgg ggccatggtg gccgcggcca ttgcgatcaa
     4501 gcggtactat gccaccgtga acgggcgtgt ggtgatcgac cggctgttgc tcaaggttcc
     4561 gattgtcgga gatctgatca gaaaagcctc cgtcgcgaag tttacccgca ccctgggaac
     4621 cttgatcacc agcggtgtgc cgttgctcga ggggttgagc atttgcgcca agacctccgg
     4681 caacaaggtg atcgaggaag cgctgatgaa tgcgcgggtg agcatcagcg gcggaaaaac
     4741 gatctccgaa ccgttggcca agtgtaacgt gtttcccaag atggtcacgc acatgatcgc
     4801 ggtcggcgag tccaccggcg cgctcgatgc catgctgggc aagatcgccg acttttatga
     4861 ggacgaagtc gatcaggcgg tcgagacgct gacctcgctg ctcgaaccga tcatgatggt
     4921 ggtgttgggt accatcatcg gatttatcgt cattgcgatg tatcttccga tcttcacgat
     4981 ggcgcaggcc atccaatagc gacgctgcac cgtcgcgtcc gttctccggc gcatggtccg
     5041 atcttcgaaa cgggccatgc gctgcacatc catcagggca acgcgccccc catgctgtcc
     5101 tcgacccttc cagttcgaac caccggtgct gagccgtcct ccagctcgac ctcccagaca
     5161 acgccactgg cagatccgta ccggacgtcg ccgggcgagc gctcgatgcc tgagctgaag
     5221 accagactcc attggctcat ggggcttcgt gtggtcgtgg tgacgctgat gctggggctg
     5281 tccctggcgt ttcagtccac tcgcggcgaa ctggtgccga ccttctccgc cctgatcatc
     5341 ctcacctatg ccaccaccat cgtctacgcg ctcttactga gacggctgag cgcccctgct
     5401 gcgctcacgg cgtttacatg ggtgcaggtg gggctggatt ttgtcctgga gactgtcctg
     5461 gtcgcgagga ccggggggat cgaaagtccc tttgcggtgc tctatgtcat tacggtgacc
     5521 gtcgccagtc tcgtcccgca tcgacgcgtg gggctggtca ccggcgccgg ttgtacgctc
     5581 ctcttcggtc tggtcacggc ggtccaatat ttcggtctgc tgcatgcctc cggctggctg
     5641 cctgcgagcc ggctggaagg cgccgagaca ctgcaaacct tcggggtcta cgggttggca
     5701 tttctggtgg tgggattttt aagcggagtg ttggcggacc agcttcgtca tgcggatcag
     5761 tcgttgcgcg agaaggaaca ggggctcaac cggctgcagg tgtttcacga gagtatcgtg
     5821 cgaagtatca gcagcggggt gttcacgacc gatgaggagg ggcggatcac gtccttcaac
     5881 cccgcggcgc atgaagtcac cggctatgcc tttgctgacg tgcaggggcg cctctggcgg
     5941 gatgtcttca attggcaccc cggtaccccc tccgaggaag ccgccttgac gtccctctct
     6001 cccttgcgat ttgaagtgga atgttcgcat gcggacggca gccgcttggt acttggaatg
     6061 acggtatctc cgctacatga acagggcaca cagcgcggcc tggtctgtgt attcaaggat
     6121 ctaacgcaac tccgctactt ggagcaggag atgcgtcggc gggaatggct ggccaatctc
     6181 ggtgaaatgt cggcgggcat ggcgcatgaa atccgcaatc cgctcggcgc gcttg
//
LOCUS       DAOQRM010000144         6193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_12710_length_6193_cov_13.331378, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000144 DAOQRM010000000
VERSION     DAOQRM010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12710_length_6193_cov_13.331378"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..581)
                     /locus_tag="PKK81_10570"
     CDS             complement(108..581)
                     /locus_tag="PKK81_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33368.1"
                     /translation="MNFRLVLTVVALGFIVLLLVIGIRTAVRWLKIHYPQRANAVLFG
                     VCLLLAGAGVLIAIEMKDQPTFRPNDLLTLQEPVVARTIVSDRESRSITCVVDLHEHL
                     GVLELDNERGSLRALVESNNTSVSLYCPIGAEVRIEAAWLHHPTITHRQADTDGP"
     gene            complement(578..2161)
                     /locus_tag="PKK81_10575"
     CDS             complement(578..2161)
                     /locus_tag="PKK81_10575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV33369.1"
                     /translation="MVSLTAMSKTPPSVTELPPPKILIVDDDPDIALVLHDLLEHVGY
                     RVSVAGTGAEALSKAKAESLSAVVLDLMLPDMDGLSVLTLLKQIDPVLPVIMLTAFVE
                     VAKKHSSLTEGAFGYLTKPYDAEELKALIRRAVGVKHLSIEAAATKQALTASEDRFRE
                     VVQTAPDAIVLADGEGKILSWNSAAERLFGYLAGEVLGESLTMIMPERYRARHVQALA
                     RVRTTGDLRLKGTVVTMHGLHKDGREFPIEMSLSSWISDGQRVHCGIVRDITARKEAE
                     ARLLRQQIEQQALLDLIPAMVWYKDAHNRILRANRRAAASIKKTVAEIEGRSTDEFYP
                     EEAERYHQDDLEVIVSGRPKLGIIEPYQTGDGEKRWVQTDKVPYLDPQGNVLGVLVFA
                     QDITERKRTEEALRESADCLRLVMESASNGIIVVDAEGMILLTNPHVDTQFGYERGEL
                     PGQPVERLMPARLRPQGSDHAGVFLIRPDDRSVSPVCECVGLRKDGTEFPFTMTLIPL
                     MTTNGLYSVVAVNQAQGIA"
     gene            complement(2336..2512)
                     /locus_tag="PKK81_10580"
     CDS             complement(2336..2512)
                     /locus_tag="PKK81_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33370.1"
                     /translation="MKNVEMTVEGTVLTIKVDLSKEFGPSASGKTTIIASTEGNVTIP
                     GREEKIGLNVYRKK"
     gene            complement(2522..2725)
                     /locus_tag="PKK81_10585"
     CDS             complement(2522..2725)
                     /locus_tag="PKK81_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNV33371.1"
                     /translation="MSVARVTEIIAASPKSFDDAIHLGIARANKTLQNVKSAWIEDQK
                     VDIEDGKITQYRVALKVTFVLKD"
     gene            2953..3663
                     /locus_tag="PKK81_10590"
     CDS             2953..3663
                     /locus_tag="PKK81_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HNV33372.1"
                     /translation="MPTEIEIQITPLFSTPLLVFTPAHHERINAELSQLILAREASVP
                     SHRDTEVIGWSSPHDLSMMDWAGSPLRELFAPVIEVAKQVTTLSDRCRHAARGPDWEV
                     VEVWANVQRNGGTNAAHSHPGSFWAGVYYVDVGEVCPTLGKGGELQIYDPRGCLPRML
                     APYLQYSMTELHDAGTSISYSPAAGQCLLFPGWLFHAVNTYRGTAPRISVAFNLDPIL
                     QQGPLSSAHADAVGGSRR"
     gene            complement(3730..4320)
                     /locus_tag="PKK81_10595"
     CDS             complement(3730..4320)
                     /locus_tag="PKK81_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33373.1"
                     /translation="MARPAMYRCRRSNNCMVVVGLALLALTAGCSPYEWRHENQWESR
                     DQIWLAEASQVKVRAAQSRVFDTADRLKTIQGVVATCQDLGFAVEVLDETLGIVSGKK
                     FDDYESLPWGDPSYHLYRDDGLLILTRQFRSWGPFYHRSNLVRLTVTVRPRGNRQVIV
                     RASAQYYLKAIESPEPYQQFFRALEQALFIQANALP"
     gene            complement(4324..4761)
                     /locus_tag="PKK81_10600"
     CDS             complement(4324..4761)
                     /locus_tag="PKK81_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33374.1"
                     /translation="MNWTQLVGAVVVMMGSVILQGCVAPEPRQELFAPTDAQMKIRSA
                     QTRTFEVKEQTVAMRGVIAALQDLGFIIERANEPLGLVTAARFAEPNYYDVLGVTVTV
                     RPQQDGKTMIRANAIYNSKPIEDPKVYQNFFAAVERSLFLVKD"
     gene            complement(4774..5349)
                     /locus_tag="PKK81_10605"
     CDS             complement(4774..5349)
                     /locus_tag="PKK81_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33375.1"
                     /translation="MQRLPAAVLLLMGLLVLPQGCVQQTQPAELFQLTPESSANRAMQ
                     TRFFDTENDQELLSASAAALQDLGFQVEESVREVGFLRAAKERSAREYGQYRNRFFIW
                     LLSLGHVVIPIDLHQKIAASLVTRPLNEAHSRQEVRIVFYRAVWKGDGQADRNYIPPG
                     EQKMEMIRDPEIYQQFFAKLSKAVFLEPHSI"
     gene            complement(5366..5767)
                     /gene="bamE"
                     /locus_tag="PKK81_10610"
     CDS             complement(5366..5767)
                     /gene="bamE"
                     /locus_tag="PKK81_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNV33376.1"
                     /translation="MRHMRWGVAVLGLCVLTGVTGCGGRQAEIKEDRLTVGRVQGEVK
                     VGMSAAQVAELLGSPNIVTTDDKRREVWIYDKVSTDRVDTASSSYAGLIILGANSSDR
                     SSSQRQRTLTIIIKYDEDKKVRDFAYNSTQF"
BASE COUNT         1259 a         1979 c         1659 g         1296 t
ORIGIN      
        1 tcaacactca atcccctttt tgtacatatt cccccggggc gttaactccc cccacccggt
       61 tacgataggc ctcctcacgt cgatgagtca agagcatggc aggcgattta cggaccgtcc
      121 gtgtcggcct gtcgatgtgt gatggtcggg tgatggagcc aggccgcctc gatccggacc
      181 tccgccccga tcggacagta caacgacact gatgtattgt tactctcgac cagcgctctc
      241 aaggaaccgc gttcgttgtc gagctccaac acacccagat gctcatggag atccaccacg
      301 caggtgatgc tccgggactc gcgatcactc acgatcgtcc ttgccaccac cggctcttgc
      361 agcgtgagca ggtcattcgg ccggaaggtc ggttgatcct tcatctcgat ggcaatcaac
      421 acgcctgccc cggccaggag cagacacacc ccgaacaaaa cagcattggc ccgctgcgga
      481 taatggatct tcaaccaacg aacagccgtg cggatgccaa tgaccaacag cagaacgatg
      541 aaacccagcg cgacgacggt caacacaagc cgaaagttca tgcgatccct tgtgcctggt
      601 tgacggcaac cactgaatac agtccgttcg tcgtcatcaa cggaatcagc gtcatcgtga
      661 atggaaactc cgtgccgtct ttccgtaagc cgacgcattc acaaacaggg ctcaccgatc
      721 ggtcgtccgg gcggatcaaa aaaacgccag cgtggtctga gccttgcggg cgcagtctcg
      781 ccggcatgag ccgttcaaca ggctgaccgg gcagttctcc tcgctcatag ccgaactgcg
      841 tgtccacatg gggattcgtc agcagaatca tcccttcggc atccaccacg atgatgccat
      901 tcgaggccga ttccatcacc aggcgaaggc aatccgcgct ctcacgcaac gcctcttcgg
      961 tccgtttccg ttcagtgatg tcctgcgcga acaccaagac cccgagcaca ttcccctgcg
     1021 ggtccaggta cggcaccttg tcggtttgca cccagcgttt ttcaccgtcc cctgtctgat
     1081 agggctcgat gatcccgagc ttcggccgcc ctgaaacgat cacctccaga tcatcctgat
     1141 gataccgctc ggcctcttcc ggataaaatt catcggttga ccggccctcg atttccgcca
     1201 cggttttctt gatggatgcc gccgcacggc gattcgcgcg caggatccga ttgtgcgcgt
     1261 ccttgtacca gaccatggcc gggatcaaat ccaacagcgc ctgctgctca atctgctgtc
     1321 gcaacagtcg cgcttccgcc tctttccgcg ccgtgatgtc ccgcacaatg ccgcagtgca
     1381 cgcgctgacc gtcggagatc caactgctca gcgacatctc aatcggaaat tcccggccat
     1441 ccttgtgcaa cccgtgcatg gtcacgaccg tgcccttcag gcgtaaatcg cccgtggtac
     1501 gtactcgcgc taaggcctgt acgtgcctgg cacggtaccg ttcgggcatg atcatcgtca
     1561 gggactctcc taacacctcc ccggccagat acccgaacag acgctcggcg gcgctgttcc
     1621 aggacaggat ttttccctcc ccatccgcga gcacaatcgc gtcgggcgcg gtctgcacaa
     1681 cttcccgaaa ccggtcttcg ctggccgtca acgcctgctt ggtcgccgca gcttcgatgg
     1741 agagatgctt cactcccacg gcacgccgga tcagcgcttt taattcttcg gcatcgtaag
     1801 gcttcgtcaa atacccgaac gctccttcgg tgagggagct gtgctttttc gccacctcga
     1861 caaacgccgt cagcatgatg accggaagca ccgggtcgat ttgtttcagg agggtgagaa
     1921 ccgagagtcc gtccatatca ggcaacatca ggtccagcac caccgcggac agagattccg
     1981 cctttgcctt ggaaagggcc tcggcaccgg tgccggcgac tgacacacgg tagccgacgt
     2041 gctccaggag atcatgcagt acgagtgcaa tatcggggtc gtcgtctacg atcaggattt
     2101 tgggaggtgg aagctcggtc acgctgggcg gggtctttga catggcggtc aacgaaacca
     2161 tgggtatcgt catacgaccg gtctgacgct tcggctgtca ttgtcctctt ttcgatgaca
     2221 agaatccagg tgtctatcga gccgctgctg aatggctcat cggataggca caggaaagac
     2281 ctcgcccggc cttcactggt cgattacccg atgaaccatt tcacctcaga gcgcctcact
     2341 tcttccggta gacattcagg ccgattttct cctctcgccc cggaatggtg acatttccct
     2401 ccgtcgaggc aataatcgta gttttccccg aggctgatgg cccgaactct ttcgacaaat
     2461 ccaccttgat ggtcagcacg gtcccctcga cggtcatctc tacattcttc atgttgctcc
     2521 cctaatcctt caggacgaat gtgaccttca acgccacacg atactgggtg atctttccgt
     2581 cttcgatatc cactttctga tcctcgatcc aggcgctttt cacattctgt aacgtcttgt
     2641 tcgcgcgagc gattccaaga tgaatggcat cgtcaaaact cttgggggat gcagcaatga
     2701 tttccgtcac acgtgcgaca gacatgatag cctccctggt ttgaatggtg gataccacac
     2761 tcggcacctc aggctaggcc gaccatacag ccctgtcaag cctgcatctc attgaacggc
     2821 ccgccggcag tgacgggtga agacgacggc tgaatctcag tagcctggga aaacacccag
     2881 gttgacttgt tgcccctcgg aggatagcgt gccccacatc gacggtcgta acacccgtat
     2941 gggctcccct ccatgccgac cgagatcgag atccaaatca cgcctctttt ctccacgcct
     3001 ctgctggtgt tcacgcctgc gcatcacgaa cgcatcaatg ccgagctctc tcaactgatt
     3061 ctggcgcgcg aggcttccgt cccgagccac cgagacaccg aagtgatcgg gtggtcctcg
     3121 cctcacgacc tgtcgatgat ggattgggca ggaagccccc tgcgcgagtt gtttgcgccc
     3181 gtcatcgaag tagccaaaca agtcacaacc ctctccgacc gatgccgcca tgctgcccgt
     3241 ggacccgatt gggaagtcgt cgaggtgtgg gccaacgttc aacgaaacgg cgggaccaat
     3301 gcagcccact ctcaccccgg aagtttttgg gcaggcgtct attatgtcga tgtgggcgag
     3361 gtctgcccga ccctaggaaa gggaggcgaa ttgcagattt acgacccacg aggctgtctg
     3421 ccgcgtatgc tggcgcccta tctccagtac agcatgaccg aattacatga tgccggaacc
     3481 agcatttcct actcccctgc ggccggacaa tgcctgctgt tccctggctg gttgttccat
     3541 gccgtgaata cgtatcgtgg aacggcgccc cgaatcagtg tggcgttcaa tctcgatccc
     3601 atcctacagc agggccccct gagcagcgct cacgcggatg ccgtcggcgg atcacgccgg
     3661 tgagagcccc ctcgccgcag cctgcgcagc tcgcacgcag gcgatgcgca tcgtggtctc
     3721 cggctctcgc tatggcagcg cgttcgcctg aatgaagaga gcctgctcga gcgcgcgaaa
     3781 gaattgctga tacggctccg gggactcgat ggccttgagg taatactgcg cactggcacg
     3841 cacgatcacc tgcctgtttc cgcgtggccg gacggtcacc gtgaggcgga caagattgct
     3901 gcggtgataa aacggccccc agctccggaa ctgccgggta aggatcagga gtccgtcgtc
     3961 ccgatacagg tgataggaag gatcacccca tggcaaggat tcgtagtcat caaatttttt
     4021 gcccgagaca atgcccagag tttcatccaa cacctccacc gcaaaaccta agtcctgaca
     4081 agtggcaaca accccctgaa tcgtcttgag acgatcagcg gtatcgaaga cgcggctttg
     4141 agccgcccgc accttgacct ggctggcctc ggcaagccag atctggtcgc gcgactccca
     4201 ttggttctca tgtcgccact catagggcga acagccggca gtcaaggcca gcaaggcgag
     4261 gccaaccacg accatgcagt tgttcgaccg gcggcatcga tacatggcgg ggcgagccac
     4321 cgactagtcc ttcaccagaa ataacgaacg ttccaccgcc gcgaagaagt tctgatacac
     4381 cttgggatct tcgatcggct tgctgttgta gatcgcattg gcacggatca tcgtcttgcc
     4441 gtcctgttgt gggcgcacgg tcacggtcac gccgagcaca tcgtaatagt tcggttcggc
     4501 aaaccgggcc gccgtcacca gcccgagcgg ttcgttcgcg cgttcgatga taaatcccag
     4561 gtcttgcaga gccgcgatga cgccgcgcat ggcgacggtc tgctccttga cctcgaacgt
     4621 gcgggtctgc gcgctgcgga tcttcatctg cgcatcggtc ggcgcaaaga gctcctgcct
     4681 cggctccggc gcaacgcagc cttgcaagat gacacttccc atcatgacca ccactgcgcc
     4741 gactagctgt gtccagttca tgaatcctcg taattaaatc gaatgcggct ccaggaacac
     4801 agccttggag agctttgcaa aaaattgctg atagatctca gggtcccgga tcatttccat
     4861 tttctgttca ccgggtggaa tgtagttgcg gtcggcctgc ccgtccccct tccacaccgc
     4921 ccggtagaag acaatccgga cttcctgccg cgaatgtgcc tcgttgagcg gccgcgtcac
     4981 gaggctggcc gcaatttttt ggtgcaggtc gatcgggatg acgacgtgcc cgagtgacag
     5041 cagccagata aagaaccggt ttcggtactg accgtactca cgagcgctac gttccttcgc
     5101 ggctcgcagg aacccgactt cccgcacact ttcctcgact tgaaacccga gatcctgcag
     5161 cgcggcagcc gaggcggaca acaattcctg atcgttctcc gtgtcgaaga aacgggtctg
     5221 catggcccga ttggcgctgc tctccggcgt gagttgaaac aattccgccg gctgcgtctg
     5281 ttgcacgcag ccctggggca agaccaacag ccccatgagc agaagaacgg ccgcagggag
     5341 ccgctgcatc aatcgaccgg ctctctcaga attgggtgga gttgtaggca aagtcccgga
     5401 ccttcttgtc ttcatcgtac ttgatgataa tggtgagggt gcgctgacgc tgcgaactgg
     5461 accgatcgct ggagttggct ccgaggatga tcaatccggc ataggaagaa ctggccgtat
     5521 cgacacgatc cgtggacacc ttgtcgtaaa tccacacttc acggcgcttg tcgtcggtcg
     5581 tgacgatatt cggcgaaccc agcagttccg ccacctgcgc cgccgacatg ccgactttca
     5641 cctcgccctg gacccgcccg accgtcaacc ggtcttcctt gatctccgct tgcctaccgc
     5701 cgcacccggt tacgcccgtc agcacacaga gcccgagcac tgccactccc catcgcatgt
     5761 gtctcatcgt atacgtctcc cttcctgaca tgaccatgtc tgcaccacgc cgttgggcca
     5821 tcttatcctg ccgacaacct cgtggcaaag gacgatctgt gcgacggaat cacatcgctc
     5881 ccctcagatt gctggacaat tcaccggagg ccgctacaat tggctgatca ggaggtactg
     5941 cgatgccgat gccggatgcc atatcgaatg atgatgttga agcagcgtgg agaacgttgg
     6001 tcgaacagac acgaacgggt gtgttactga cactccggaa cgggcatccc ttcggctccc
     6061 atgtacccta cctgctcgga gacgactggg ctcgcgtgta tcttcacctc agccggctgg
     6121 ccttacatac ccagcatctc cttgaagacg cccgtgtcag cctgtttctc gccgagccgg
     6181 acaagccagg caa
//
LOCUS       DAOQRM010000145         6047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_13182_length_6047_cov_15.846295, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000145 DAOQRM010000000
VERSION     DAOQRM010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13182_length_6047_cov_15.846295"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..999
                     /locus_tag="PKK81_10615"
     CDS             76..999
                     /locus_tag="PKK81_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNV33377.1"
                     /translation="MSPTGQLTPTGLDIRQYLERKREEVDQYLQSVIPSAETMPTTLH
                     ESMRYSLFAGGKRVRPILAIAAAEAVGHAGKAVLPVASSLELIHTYSLIHDDLPAMDN
                     DDFRRGKPTNHKVYGEAMAILAGDALLTMAFELCARANAEHSLDAARQVQLIRELAVG
                     SGNLGMVGGQVLDIQAENKDIDLATLQSIHKHKTGMLIRAAVRMGAITAGAMPAQLDD
                     LTFYAEQIGLAFQIADDVLNVTGTREELGKNPNTDAQRGKKTYPTFYGVEGAKQLAEE
                     CVTRANDRLASFGSKADPLRELARYITSRKN"
     gene            1106..2089
                     /locus_tag="PKK81_10620"
     CDS             1106..2089
                     /locus_tag="PKK81_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV33378.1"
                     /translation="MKALVTGATGFVGGAVARALVKTGIEVRVLSRKGADLQNLAGLP
                     VEQVDGDLRNPESLRRALTGCRQLYHVAAHYALWAKDPSIFYDINVTGTRTLLEAARR
                     VGIERTVYCSTIGAIGLPPGGGPGTEETPVSLEQMAGHYKRSKYLAEQEVLKLAREGL
                     PVVIVNPSAPVGEADVKPTPTGQIIVDFMKGRMPAYIETGMNLIDVDDVAQGHLLAMQ
                     KGRQGERYILGNKNLLLNDVFQILSRITGVKAPTIKLPRLAILPLAYANQWISNLTGV
                     PPRIPLEGVKMAKYKMHYDCSKAIRELGLPQTPVEVALEKAVRWFRSHGYA"
     gene            2300..3112
                     /locus_tag="PKK81_10625"
     CDS             2300..3112
                     /locus_tag="PKK81_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNV33379.1"
                     /translation="MEILLLFFKTILLRPYVFAFLAAFLVSAIALIGWPRTWRFWLIS
                     WITAFVCEFSSTRNGIPFGWYFYNGSTVGQELYFSNIPFMDSISFSFLLFASYCLALL
                     LLLPVQSSSCADTTSRLPDLKFDLPLRTSWPVFALTVLFFAFIDMVIDPVALRGDRWF
                     LGKIYYYPDPGVHFGVPMANYVGWAVVGAISLLIYFPLDRRLQHPLPDHRPSTTHRLL
                     LGGGLYYGVLAFNLAMTFWIGETLQGTTGLLMYVPVTAILLLRLLAPATQSA"
     gene            3220..3837
                     /locus_tag="PKK81_10630"
     CDS             3220..3837
                     /locus_tag="PKK81_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV33380.1"
                     /translation="MKKICGLLMLGFLATLGILGWFGYQSYSTGFSAKAEPNELEVLI
                     ARQLRHLAIPYENRQLRNPLPVTQELMKEARAHFADHCASCHANNGSGDTVIGKNVYP
                     KAPDMRLPETQTMSDGELFFIIQNGIRFTAMPGWGTGDPAKDSGSWQLVHFIRHLPSL
                     TEEELQEMTGLNPKTKKELQEEALFDQFLGGDDGAASKAAGSHHH"
     gene            3860..4180
                     /locus_tag="PKK81_10635"
     CDS             3860..4180
                     /locus_tag="PKK81_10635"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNV33381.1"
                     /translation="MTFLRLFIAAFLLICAPAVVAAHGSGQHVLGVVAAIDATHIEVK
                     TPKGQVVSVRITDKTQYKVRNLRRPKSPPQVGDRVVIEAEKGTDGLTATEVHYSDSKP
                     KEAQ"
     gene            4354..4812
                     /locus_tag="PKK81_10640"
     CDS             4354..4812
                     /locus_tag="PKK81_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNV33382.1"
                     /translation="MRVVQLQGSQHSASNSGGLRPIVMRVLIMGLAVFLAVTIVPGIE
                     SESLGAGLAAVLVLTVLNTLIRPLLYLLALPLIVVSLGLFMVVINALLLQLTAALVKG
                     FTVVGFGASFWGALVISLVSSLLNMILVVEHTRVERHDHPQRPPTIINPD"
     gene            5104..>6047
                     /locus_tag="PKK81_10645"
     CDS             5104..>6047
                     /locus_tag="PKK81_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV33383.1"
                     /translation="MSPPAESEKKAAADTHLQRTLTSVVNGLPADAALVAIFHQEQGP
                     LTTQVHRGFTPRDVQSIVRTLSSQKVLTSVPAGNDPEASRTMRLRLVTPGAKSLLGVP
                     LRHRQRTFGFLVIGRKDNAVYAKKDKTMLEQAGDDITKALERDSLFDLNVLLSRPLVV
                     QEPQPVIATPDVYNAPTSHATPEIQEKIVALLNELGQTLAFDRAWIGAYDPLAGNVEV
                     LGIAGEQKTDPKDQKKDLKAGQRLTLDASAAGWVVRHRKPRVDHDLASTQGRFLDHKH
                     LYKDRFQSSLVLPFFLRGQVGGTITLASKEADRFAVTDA"
BASE COUNT         1229 a         1954 c         1697 g         1167 t
ORIGIN      
        1 ccgtttgaca ctccccgaac ccctttgtta ctctctcgcg cacatgaaga cgcacagccc
       61 cctgagcact ccggcatgag ccccacgggc caactgaccc cgaccggtct ggacatccgc
      121 cagtacctcg aacggaaacg cgaggaggtg gatcagtatc tgcaatcggt cattccgagt
      181 gccgagacca tgcccacgac gctgcatgag agcatgcggt acagcctgtt cgcaggcgga
      241 aagcgggtcc ggcccattct ggccattgcc gccgccgaag cggtcggcca cgctggcaaa
      301 gccgtcttgc ccgttgcgtc ctcactggaa ttgatccaca cctattccct cattcacgac
      361 gatctcccgg cgatggacaa cgacgatttt cgccgcggga aaccgaccaa ccacaaggtc
      421 tacggcgaag cgatggcgat cctggccggc gacgcgctgc tgaccatggc gtttgaactc
      481 tgcgcccgcg cgaatgccga gcacagtctc gatgccgcgc gccaggtcca actgatccgc
      541 gaactggccg tcgggtccgg caacctcggc atggtcggcg ggcaagtcct cgacattcag
      601 gccgaaaaca aggacatcga cctcgccacg ttgcagtcca tccacaagca caaaaccggc
      661 atgctgattc gagccgccgt gcggatgggc gcgatcaccg ccggggcgat gcccgcacaa
      721 ctcgacgacc tgaccttcta tgccgagcaa atcggcctgg ccttccaaat cgccgacgat
      781 gtgttaaacg tcaccggtac gcgcgaggaa ctcggcaaga atcccaacac cgacgcccaa
      841 cgcggcaaaa aaacctaccc gacgttttat ggcgtcgagg gagccaagca actggcggaa
      901 gagtgcgtca cccgagccaa cgaccggctc gcctccttcg gttcgaaagc cgatcctctc
      961 cgcgaactgg cccggtatat cacctcgcgg aaaaattaac cgtgaggcgt gaaacgtaag
     1021 acgtgaaacg cgaccccctc ctccagacca tcatctctcc caacatacct ttcacctttc
     1081 acgtttctcc ttttacgtct tccccatgaa ggctctcgtc accggcgcaa caggttttgt
     1141 cggcggggcc gtggcgcgcg ccctggtcaa gacaggcatc gaggttcgcg tgctctcgcg
     1201 gaagggcgca gacctccaga acctggccgg gctcccggtc gaacaggttg atggagacct
     1261 acgcaacccc gagtcgctcc ggcgtgccct caccggttgc cggcagctct atcacgtggc
     1321 ggcccattac gcgctctggg cgaaagaccc ttccattttt tatgacatca acgtcaccgg
     1381 cacgcgcacc ctgctggagg cagcccgcag ggtcggcatc gagcggaccg tctattgcag
     1441 caccatcggc gcgatcggct tgccgccggg cggaggacca gggacagaag aaacgccggt
     1501 gtcgctggaa cagatggccg gccattacaa gcgttcaaaa tatctggccg agcaggaagt
     1561 gctgaaactc gcgcgcgaag gcttaccagt cgtcatcgtg aaccccagcg cgccggtcgg
     1621 ggaagccgat gtgaagccca cccccaccgg tcaaatcatc gtggacttca tgaagggccg
     1681 catgcccgcc tacatcgaaa ccggcatgaa cctgatcgac gtggacgacg tggcccaagg
     1741 ccacctgctc gccatgcaga agggccggca gggcgaacgc tacatcctcg gcaacaagaa
     1801 tctgctcctc aatgacgtgt tccagatcct cagccggatc accggggtga aggccccgac
     1861 catcaaacta ccgcgcctcg ccattctgcc cctggcctat gcgaatcagt ggatctcgaa
     1921 cctcacgggt gtgcccccgc gcattccgct cgaaggcgtg aagatggcca aatacaagat
     1981 gcattacgat tgcagtaaag cgatccggga actcggcctg ccccaaacac cggtcgaggt
     2041 ggcgcttgaa aaggcggtgc gatggtttcg ttcacatggc tacgcttagt cggatgctga
     2101 aaaagccccc caactgcgtt ctcggcttga caggctcctc aacataccca cggagaaaga
     2161 gctgtcccgg cagctcgggg tgggtgggta agaactggca cgcctccggt cttgtcgtcg
     2221 cctggggcag ggccgtgtct tgacgcgccg tggtggacgg gtaagaacgt gatttttttg
     2281 agcatccgtt gatcccccga tggaaatcct tctccttttc tttaaaacca ttctgctccg
     2341 cccctacgtt tttgccttcc tcgccgcgtt tctggtttca gccattgcgt tgatcggctg
     2401 gccgcggacc tggcgcttct ggctcatcag ctggatcacc gccttcgtct gtgaattttc
     2461 ttcgacgcgc aacggcattc ccttcggctg gtacttttac aacggttcca cggtcgggca
     2521 ggaactgtac ttctccaaca tccccttcat ggactcgatt tcgttctcgt tcctgctgtt
     2581 cgccagttat tgcctggctc tattattgct gctgccggtc caatcgagtt catgcgccga
     2641 cactacgtca cggcttcccg atctgaagtt cgatctgccg ctccggacaa gttggccggt
     2701 ctttgccttg accgtgctgt tcttcgcgtt catcgatatg gtcatcgatc cggtcgcgtt
     2761 gcgaggtgac cgttggtttc tgggaaagat ttattactac ccggatcccg gcgtgcattt
     2821 cggcgtcccg atggcgaact acgtgggctg ggcggtcgtc ggcgcgatct ccctcttgat
     2881 ctatttcccc ctggaccgac ggctccaaca ccccttgccg gatcaccgtc cttcaaccac
     2941 ccatcgcctg ctgctgggcg gcggactcta ttacggagtg ctcgccttta acctggccat
     3001 gacgttctgg atcggcgaaa cacttcaagg caccaccggc ctgctgatgt atgtgccggt
     3061 gacggccatc ctgttgctac gcctgctcgc gcctgcaaca caatctgcgt aaacacaccg
     3121 gaagcagggc cgcggcgaac gcgccatcat tggcattcat tcaatcgagt ctgtatattg
     3181 ttctatggcg ttgtgcctga actggaaggt gtgtccccga tgaaaaaaat ctgtggactg
     3241 ctgatgctgg gcttcctcgc caccctggga atcctcgggt ggttcgggta tcagtcctac
     3301 agcaccggat tcagcgccaa ggctgagccg aacgagctgg aggtgctgat cgccagacaa
     3361 ctccgccacc tggccattcc gtatgagaat cggcaactcc gcaatccgct gcccgtcacg
     3421 caggagctga tgaaggaggc ccgggcccat ttcgccgacc attgcgcctc ctgccacgcc
     3481 aacaacggca gcggggacac cgtgatcggg aaaaacgtct accctaaagc gcctgacatg
     3541 cgtcttccgg agacgcaaac catgtccgac ggagaattgt tcttcattat tcagaacggc
     3601 atacgcttca ccgccatgcc cggctggggg accggcgatc cggccaagga cagcggcagc
     3661 tggcaactcg tgcatttcat ccggcatcta cccagcctga ccgaagaaga attgcaggag
     3721 atgaccggct tgaatccaaa gaccaagaaa gaactccagg aagaagccct gttcgaccag
     3781 ttcctgggcg gggacgacgg cgcagcctcc aaggccgccg gctcgcatca tcattaattc
     3841 gcgaacgaaa ggacgacccg tgacattcct tcgactcttc attgccgcgt tccttctgat
     3901 ttgcgccccg gccgtggtgg ccgcccatgg gagcgggcag cacgtgctcg gcgtcgtggc
     3961 cgccatcgac gcgacccaca tcgaggtcaa aacccccaag ggccaggtcg tgtcggtgcg
     4021 aatcacggac aagacgcaat acaaggtgcg gaaccttcgc cgccccaaga gtccgccaca
     4081 ggtaggagac cgcgtggtca ttgaagccga gaagggcacc gacggcctga ccgctaccga
     4141 agtgcattac tcggactcca aacccaaaga ggcacaatag atagccaaga gccgccggtt
     4201 acggagaggg cggcaatggt agaggatgtt caaaacggcc gttcagcaag gccgcagcga
     4261 acgaagaggc gaggcgtaca cttcggtacg ttgagcctct gaatgatgcg agaacgaagc
     4321 tggcggacgt tttcaacatc ctgagagaac cgtatgcgag tcgttcagtt gcaggggagt
     4381 cagcacagcg cgagcaacag tggtggcctg cgccctattg tcatgcgggt gctgatcatg
     4441 ggactggccg tctttctcgc cgtgaccatc gtgcccggca tcgaatcgga gagcctcggt
     4501 gccggcctgg cagcggtgct cgtgttgacg gtgctgaata cgctgattcg gccgctgctc
     4561 tacctgctgg cgctcccgct gatcgtcgtc tcactcggcc tgttcatggt ggtgattaac
     4621 gccctgttgc ttcagctgac cgccgccctc gtcaaaggtt ttaccgtggt tggattcggc
     4681 gcgtccttct ggggcgccct cgtcatcagc cttgtgagca gtctgctcaa catgatcctg
     4741 gtggtagaac atacccgtgt ggagcggcac gatcatcccc agcgcccacc cacgatcatc
     4801 aatccggatt agcagtggag agctgatagc gtatggcgta tagcacaaac ggcgggaggc
     4861 cccatggacc ttgccataag ctattggcca tgtgcgcttc aaattcatgt gcttccttga
     4921 gttaccttga tccggctgct acaatgccgc ccgacgctcg ctattcaccg ccgagagcgc
     4981 acaacctata acgagcacca tgttccgaac atgctgcggg ttatgactca ccatctgagt
     5041 catccatacc gggacagaat cggtagcccc ctgcgtagtc ggtagcatcc tgcatagagg
     5101 tcgatgagtc ctccagccga atcagaaaaa aaagccgccg ctgatacgca tctgcaacgc
     5161 accctgacct ccgtcgtcaa cggcttaccc gccgatgccg ccctggttgc gatttttcac
     5221 caggaacagg ggccgctcac gacgcaggtt caccgcgggt tcacgccacg cgatgttcaa
     5281 tcgatcgtcc gcacgctgtc ctcgcagaag gtgctgacct ccgtccccgc cggcaacgat
     5341 cctgaggcct cacgcaccat gcgccttcgc ctggtcaccc cgggggccaa gtcgctgctg
     5401 ggcgtgccgc tgcggcaccg tcagcgcacc ttcggcttcc tggtgatcgg acggaaagac
     5461 aatgccgtct atgccaaaaa agacaagacg atgctggagc aggccggcga cgacattacc
     5521 aaggccctcg aacgggacag cctcttcgat ctcaacgtcc tgctgagccg tccgctggtc
     5581 gtgcaagagc cgcagccggt tatcgcgacc ccggatgtct acaacgcgcc gacctcccat
     5641 gcgaccccgg agatccaaga aaaaatcgtt gcgctgctca acgagctggg ccagaccctg
     5701 gccttcgacc gcgcctggat cggagcctat gacccgctcg ccggcaacgt cgaagtactc
     5761 ggcatcgccg gagaacaaaa gaccgacccg aaagaccaga agaaagatct caaagcgggc
     5821 caacgcctga cgctcgacgc ctccgctgcc ggatgggtgg tgcgccaccg caagccgcgt
     5881 gtcgatcacg atctcgcttc gactcagggc cgcttcctcg accacaaaca tctgtacaaa
     5941 gaccggttcc agtcgtcgct ggtgctcccc ttcttcctgc gcggccaagt cggcgggacc
     6001 atcacgctgg cctcgaaaga agcggaccgt tttgcggtga ccgatgc
//
LOCUS       DAOQRM010000146         5976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_13431_length_5976_cov_11.946631, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000146 DAOQRM010000000
VERSION     DAOQRM010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13431_length_5976_cov_11.946631"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /gene="ribB"
                     /locus_tag="PKK81_10650"
     CDS             complement(<1..584)
                     /gene="ribB"
                     /locus_tag="PKK81_10650"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNV33384.1"
                     /translation="MATPFNSIEDAIKDIKKGKFIILVDDEDRENEGDLVMAAGKVTP
                     HAVNFMAKHARGLICLALTPERVEELQLAPQAAENTATFGTAFTVSIDARKGITTGIS
                     AADRATTIHVAIDSRSTPGDLARPGHIFPLKSQVGGVLRRAGQTEGSVDLARLAGLYP
                     AGVICEIMNEDGTMARVPELTKFARRHKLKMVTI"
     gene            complement(650..1879)
                     /locus_tag="PKK81_10655"
     CDS             complement(650..1879)
                     /locus_tag="PKK81_10655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV33385.1"
                     /translation="MSLSTLGVALRSGHWPTLAGAWLHLTVSFMVWLLFGALAVAIGQ
                     ELGLTATQQSVLVALPLLGGAALRITAGWACDWYGGKRTGLLVLGLQLLAVIWAALGG
                     RSYVELLGIALLLGAGGASFAVAMPVASRAYPATHQGLVLGLVASGNIGTVLVLLLAP
                     RWETALGWHWACGIMAVPILIAIGLFTTVVQSEPTVRTAGEGAWWQAAWAMARVPSVY
                     WLCMIYGVTFGGFVGLTSFLPVLLHDQYGSDPIVGGSIAALCGFTGSLIRPVGGYVAD
                     RWGGLPVLVGVFIALALMTVLAGSLPALPWAVGLLVVTIAVMGFGNGVIFQIVSEWFP
                     KDIGLASGLVGAAGGIGGFLVPIGFGLLRESTGTFLFGFVGLAVVSAAAAMTVAPALR
                     WRPQAVAQAPSPGGFSR"
     gene            2095..3117
                     /locus_tag="PKK81_10660"
     CDS             2095..3117
                     /locus_tag="PKK81_10660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HNV33386.1"
                     /translation="MTQRKHTHNSDSHADLDEDTDAIEPEALEPTDEEVTEEHDAAGH
                     QPSKEPQEAPLSTSLVPVTTLQQYLTEIRRYPYLSKEEELRLFEEYQLRGSRDAAMKL
                     ILANLRVSVSIASEYLHTGADHMDLIQEGNVGLLQAIKKFDPTKNVRFYAYAAWWARA
                     YILRYLLNTYRLIKVGTTQDQRKLFYNLKKEKAKLEREGFAPDSKLLADRLNVRERDV
                     IEMDQRLGSWELSLDQPIGQENDGTLMDILPAHEQPADERLADTQLKALFRRKLAEFT
                     KTLDEREEDILRNRILSETPLTLEDMGAKYGITKERTRQLEARIIKRLRDYIKKDVKD
                     FDRLRM"
     gene            3119..4657
                     /locus_tag="PKK81_10665"
     CDS             3119..4657
                     /locus_tag="PKK81_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33387.1"
                     /translation="MATFFRIAIAGFLLAVVGETVSPVRSLADSPITRENTRPGGTDW
                     ILTDPAGHEVEGYASATSIQRGDKLHFYIHSTDPRITVAIYRMGWYAGAGARLVQGGI
                     SLPGVRQPMPSADPVTGLIECDWQVSYTLNTATDDPGEWLSGVYLAKLTGTSSGKQSY
                     IIFVVREDDRPADFLFQSSVTTFQAYNNWGGKSTYPSNSRDEQWARKVSFNRPYAASQ
                     HPRGASGTGAGEFLTAMSIHPTRSLSTAGWEYNMVRWLERNGYDVTYSTDIDTHSRPD
                     FWKGHKAWLSVGHDEYWSDEMRRHVEAARDQGLGLGFLSANTCYWQIRLEPSPLTTEP
                     NRTMVSYKEVALTEDPYALDGDPSNDHLITVQWRDQPLNRPEHKLLGVMFETVPVDGD
                     LVITRPSHPLFSGIPLPPDHRLPGLLGYEIDRAFPDGPAKLEILAHSPHQRDGQVVYG
                     DMTLYQAPSGAHVFATGTIQWSWGLDDYNAPALRTARTSEAAQQITRNILELLARKTL
                     SAAQ"
     gene            4969..5271
                     /locus_tag="PKK81_10670"
     CDS             4969..5271
                     /locus_tag="PKK81_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNV33388.1"
                     /translation="MATEAKDKKSTAAKNFQPLGDRLFVTYTEEMERTSGGIYVPDSA
                     KEKPQRGIVQAIGKKVENIKVGDQVLFDKYSGSKLRIEDEECLILKEEDILGIFTN"
     gene            5328..>5976
                     /locus_tag="PKK81_10675"
     CDS             5328..>5976
                     /locus_tag="PKK81_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNV33389.1"
                     /translation="MMAKQLLYSEAARAAILRGVNQLADAVKATLGPKGRNAILDKKF
                     GAPTITKDGVTVAKEVELKNPYENMGAQLVREVASKTSDTAGDGTTTATVLAQAIYRE
                     GVKNITAGANPMEIQRGINKAVEVVIGELKKLSKPCQNKTEISQVGTISANNDKTIGD
                     LIAEAMEKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTNAERMEAVMD"
BASE COUNT         1367 a         1970 c         1644 g          995 t
ORIGIN      
        1 ttgatcgtga ccattttcaa cttgtgccgc ctggcaaact tggtcagttc cggcacacga
       61 gccatcgtgc cgtcttcgtt catgatctcg cagatgacgc cggccgggta gagtccggcc
      121 aatcgtgcta ggtcaaccga cccttccgtc tgccctgcgc gccgaagcac cccgccgact
      181 tgtgacttca gggggaaaat gtgaccgggg cgcgccaagt cgcccggtgt ggaacgcgag
      241 tcgatcgcga catgaatggt cgtggcgcga tccgccgccg agataccggt ggtgatccct
      301 ttgcgagcgt cgatggagac cgtgaacgcg gtgccgaagg tggccgtgtt ctccgccgcc
      361 tgcggcgcca gctgcaactc ctccacccgc tccggagtca gcgccagaca gatgaggcca
      421 cgcgcatgtt tggccatgaa gttgacggcg tgcggcgtga ccttgccggc cgccatcaca
      481 agatcgccct cgttttcacg atcttcgtca tcgacgagga tgatgaactt ccccttcttg
      541 atatccttga tcgcgtcttc gatcgaatta aacggtgtcg ccataatccc tcgatggtgc
      601 aggccagcct gcttcggtgg cccagctaac tcttttcaaa ccacccctgt caacgggaaa
      661 aaccaccggg gctcggggcc tgcgcgacgg cctgcggacg ccaccgcagg gccggagcca
      721 ccgtcatcgc cgcggcggca ctcacgaccg ccaatcccac gaacccgaac aggaacgtgc
      781 ccgtactttc ccgcagcaac ccgaacccga tgggcaccag gaacccgccg atcccgccgg
      841 cagcgcccac caggccggag gccagaccga tgtcctttgg aaaccactcg gagacgattt
      901 gaaaaatcac cccattcccg aaccccatca cggcaatcgt caccaccagc aatcctacgg
      961 cccagggtaa ggccggaagc gaccccgcca gcaccgtcat caaagcgagt gcaataaaca
     1021 ccccgaccag caccggcagc ccgccccatc gatccgccac atatccgccg accggacgaa
     1081 tcagacttcc cgtgaatccg cagagcgcgg cgatggaacc accgacgatc ggatcgctcc
     1141 catactgatc gtgcaacaac accgggagaa aactcgtcag cccgacgaat ccgccgaacg
     1201 tgacgccgta aatcatacaa agccaataca ccgacggcac ccgcgccatc gcccaggccg
     1261 cctgccacca ggccccctct cctgccgtgc gcacagtcgg ctcgctctgc accaccgtgg
     1321 taaagagacc gatcgcgatc agaatgggga cggccatgat tccgcacgcc cagtgccatc
     1381 cgagcgccgt ttcccatcgc ggagcgagca gcagcaccaa cacagttccg atatttcccg
     1441 aggccaccag gcccaacacc agaccttgat gggtcgcggg ataggcccgg ctggccaccg
     1501 gcatcgccac cgcaaaactc gcccctccgg caccaagcag gagcgcaatg ccgaggagct
     1561 ccacataact ccgccctccg agcgccgccc agatgaccgc gagcagttga agacccagca
     1621 ccagcagacc ggtccgcttc cccccatacc agtcacaagc ccagccggcg gtaattcgca
     1681 atgccgcgcc gcccagcaac ggcagtgcca caagtacact ctgctgggtc gcggtcagcc
     1741 cgagttcctg tccgatcgcc acggccaagg ccccgaacaa cagccacacc atgaaactca
     1801 cggtgagatg caaccaggcg ccggccaggg tcggccaatg cccgctgcgc aaggctaccc
     1861 ccaacgtgct gaggctcatc aacgacttca ttccgcgccg gctgtgtcca tgccatccgt
     1921 gtggtttttc agcatcatcc acgcactata cgaggcgcga tttcagagac gcaagggagc
     1981 cgcggacgag ccggcctatc acgttgttgc ctggcacccg acatctggta tagtcagccc
     2041 ttgctcggaa ttcatgatga gcgtcctggc tggtagccca cagtttcaac ccctatgaca
     2101 cagcgcaaac acacgcacaa ctcggattct cacgcagacc tggacgaaga caccgacgcc
     2161 atcgaacccg aggcccttga gccgacggac gaagaggtca cggaggaaca tgacgcagcc
     2221 ggccaccagc cgtcgaagga accgcaggag gccccgctct caacctccct ggtcccggtc
     2281 accacgctcc agcaatacct gacagaaatc cgtcgttatc cctacctgag caaagaagaa
     2341 gaactccggc tcttcgagga atatcagctc aggggcagtc gcgatgcggc catgaagctc
     2401 atcctggcca acctgcgggt ctccgtctct attgcgtcgg agtacctcca taccggcgcc
     2461 gaccacatgg atttgattca ggaaggcaac gtcggcctgc tccaggcgat caaaaaattc
     2521 gaccccacga agaacgtccg cttttatgcc tacgcggcct ggtgggcaag ggcctacatt
     2581 ctccggtacc tgctgaatac ctaccgattg atcaaagtcg gcacgaccca ggaccaacgc
     2641 aagctcttct acaatctgaa aaaggaaaag gccaagctcg aacgggaagg cttcgcgccg
     2701 gacagcaaac tcctggcgga tcgcctcaac gtgcgcgaac gcgatgtcat cgaaatggat
     2761 caacggctgg gcagctggga actgtcgctc gaccagccca tcggccagga aaacgatgga
     2821 acgctcatgg atatcctgcc ggcgcatgag cagccggcag acgaacgact ggccgatacc
     2881 caactcaaag cgctcttccg gcggaagctg gccgaattta cgaagaccct cgatgaacgg
     2941 gaagaagaca tcctgcgcaa ccgcatcctc tccgagacac cgttgaccct ggaagacatg
     3001 ggcgccaaat acggcatcac caaggaacgc acccggcagt tggaggcgcg aatcatcaaa
     3061 cgcctccgcg actacatcaa gaaggatgta aaggactttg accgccttcg gatgtaggat
     3121 ggcgaccttc ttcaggatcg ccatcgccgg cttcctactg gcggtggtcg gcgaaaccgt
     3181 ttcacccgtc cgctcactcg ccgactctcc cattacccgg gaaaataccc gaccgggcgg
     3241 taccgactgg atcctcaccg acccagccgg ccatgaggtc gaagggtatg cctcagccac
     3301 cagcatccag cgcggggaca agctccactt ctacatccac agcaccgacc cacgtatcac
     3361 cgtcgccatc tatcggatgg gttggtatgc gggcgccgga gcccggttgg tccaaggcgg
     3421 catttcgctg ccgggagtgc gacagccgat gccgtcggca gacccggtga ccggactgat
     3481 cgaatgcgat tggcaggtgt cgtacacgct gaacacggca acggacgacc cgggtgaatg
     3541 gctgagcggc gtctacctcg ccaagttgac cggcacgagc agcggcaaac aaagctacat
     3601 tatcttcgtc gtcagggaag acgaccggcc ggccgacttt ctgtttcagt ccagcgtcac
     3661 aacattccag gcctacaaca actggggcgg caaatcgact tacccctcga acagccgcga
     3721 tgaacaatgg gcgcggaaag tctcgttcaa ccgtccctat gccgccagcc aacacccgcg
     3781 gggcgccagc ggaaccggag cgggagagtt cctgaccgcc atgtcgatcc acccgacccg
     3841 ctccctctcg accgccgggt gggaatacaa catggtccgt tggctggagc gcaatggcta
     3901 cgacgtcacc tacagcaccg atatcgacac ccatagccga ccagatttct ggaagggcca
     3961 caaggcctgg ctgtcggtcg gccacgatga atactggtcc gatgaaatgc gacgccatgt
     4021 ggaggcggcc cgcgaccagg gcctgggact cggcttcctt tccgccaata cctgttactg
     4081 gcaaatccgg ctcgaaccca gccccctcac gactgaaccc aatcggacca tggtgtccta
     4141 caaggaggtg gctctgacgg aggatcccta tgccttggat ggcgatccgt ctaacgacca
     4201 cctgatcacc gttcaatggc gcgatcagcc gctgaaccga cccgaacaca agctactcgg
     4261 cgtgatgttc gagaccgtcc cggtggacgg agatctcgtc atcacccgcc cctctcatcc
     4321 gctgttcagt ggcatccctt tgcccccgga ccatcgcctg cccggcctgt tgggatacga
     4381 gatcgaccgg gcattccccg acgggccggc gaagctggag atccttgccc attctcccca
     4441 ccagcgggat ggacaggtcg tgtatggcga tatgaccctc taccaagccc cgagcggggc
     4501 ccacgtgttt gcgacaggaa ccatccagtg gagttggggc ctggacgatt acaatgcgcc
     4561 ggcactgcga accgcccgta ccagtgaggc cgcccaacag atcacccgca atatcctgga
     4621 gctgctggct cggaagaccc tctcggctgc ccaataatcg ggatttcgcc ttccttctga
     4681 ctccgacttg agcccttggc gcaaccttgc ggcgagcttc tccaaaatat agtgggaaga
     4741 ctcagaaagc atgctctaga gaatcaatag cttaggggcc attatcccat tgcatctcac
     4801 cgtagaataa ttttatagtc catccattga cttgcctctt gctagcgcta tctatgtctc
     4861 gggtttactg caggtagccc ttgcggtaac acgtcaaagc tgatttacca gctttcacac
     4921 aactgtacat tagggcctcg acgctcatca ccagaaggag gattcggtat ggctacggaa
     4981 gcgaaagaca agaagtccac ggctgcgaag aattttcagc cgctcggtga ccgtttgttc
     5041 gtcacctaca ctgaggaaat ggaacggacc tccggcggga tctatgtccc tgactctgcg
     5101 aaggaaaagc cgcagcgggg tatcgtgcag gccatcggga agaaggtcga gaacatcaag
     5161 gtcggcgacc aggtgttgtt cgacaagtat tccggcagca agcttcggat cgaagacgaa
     5221 gagtgcctca tcctcaagga agaagacatc ctcggcatct tcaccaacta attctgtctg
     5281 acccccaaac acctgaaaaa cgactactag acgattaacg gaggacgatt atggcaaagc
     5341 aactactgta tagcgaagcg gcacgggcgg ccatcctgcg cggggtgaac cagctcgccg
     5401 atgccgtcaa ggcgacgctc ggtcccaagg gccggaacgc gattttggat aagaagttcg
     5461 gcgccccgac gatcaccaag gacggcgtga ccgtggcgaa ggaagtcgaa ctgaagaacc
     5521 cgtacgagaa catgggcgcc cagctggttc gcgaagtcgc gagcaagacg agcgatacgg
     5581 ccggcgacgg aaccaccacc gccaccgtgt tggcccaggc catctaccgg gaaggcgtca
     5641 agaacatcac cgctggtgcc aacccgatgg aaatccagcg cggcatcaac aaggccgtgg
     5701 aagtcgtgat cggcgagctg aagaagctca gcaagccttg ccagaacaag accgaaatct
     5761 cccaagtcgg taccatttcc gccaacaacg acaagaccat cggcgacctc atcgcggaag
     5821 cgatggaaaa ggtcggcaag gatggcgtga tcacggtcga agaagccaag tcgatgacca
     5881 cgtcgctgga cgtcgtcgag ggcatgcagt tcgatcgcgg gtacatctcc ccctacttcg
     5941 tgaccaacgc cgagcggatg gaagccgtca tggacg
//
LOCUS       DAOQRM010000147         5956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_13485_length_5956_cov_14.281139, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000147 DAOQRM010000000
VERSION     DAOQRM010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13485_length_5956_cov_14.281139"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKK81_10680"
     CDS             <1..275
                     /locus_tag="PKK81_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNV33390.1"
                     /translation="PKELAAAITVEASELLEIFQWKSHDEVARLLESPSRERVEDEIA
                     DVVILLSYLCHDLGLDVNAAVLSKLKKNEAKYPVEKSYGNARKYDE"
     gene            308..1027
                     /locus_tag="PKK81_10685"
     CDS             308..1027
                     /locus_tag="PKK81_10685"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HNV33391.1"
                     /translation="MMLRSVLLFVAVSVAFAGCGATRHARSVEPSGFLQDLYAQMREG
                     KGDEALLVYRNPAVDWPAKAAYNKILLDPIMIWRGKDSKVDGLDPKEAQVIAGSFYAL
                     LYQELAKDYEMVSEPGPKMFRMQAAHTDAEQSYPALDIVSSVPAPFNIAFVASTIKTL
                     TTGKPLFKGTASIEGKLMDAESGEILAAAVDRRVGGRFLDAEVFDSWNDVHGALKYWS
                     QLTRFRLCQLRKQTNCVSPGS"
     gene            1272..1436
                     /locus_tag="PKK81_10690"
     CDS             1272..1436
                     /locus_tag="PKK81_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33392.1"
                     /translation="MAQITEVAPDLFRITTFLEPFNLQFSQFLVRDAEPLLYHTGPRA
                     LFPAVKETVA"
     gene            1455..2000
                     /locus_tag="PKK81_10695"
     CDS             1455..2000
                     /locus_tag="PKK81_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV33393.1"
                     /translation="MRWIGFSHFEADECESLPEWQQLAPQSDAVCSLVSKLVSVDDCL
                     ALRPAKGMTDGEVLETGKYRFRFLATPHVPHCWESGLMFEETQRTLLCSDLLHQNGNV
                     EPVTQSDVVGRSRDVLVEYQQGPLANYMPYCTLTDPTLQRLAALQPKTVATMHGSLYV
                     GDGGKALRDLATVFREVLGAA"
     gene            2124..2771
                     /locus_tag="PKK81_10700"
     CDS             2124..2771
                     /locus_tag="PKK81_10700"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV33394.1"
                     /translation="MRIRHVVIAFSLAGCLTIPGWGVIRAEPSFDQTAGYILNIVRAF
                     RTAYVLHVVEHARDAGLSPREDWKTDAHFLPLPAQFVKEAAEQVEGLEIGLISLTPLN
                     PANRPRTDAEMTALLQLEKDRQRGVIGFVDGDEFKAVSADLALVRSCVDCHNQHPRAV
                     RKNFQQWDVMGALVVRLKRTVEGEGQALPPEPPKRAPGLLEGPPPPPTITPPWVR"
     gene            complement(2870..4216)
                     /locus_tag="PKK81_10705"
     CDS             complement(2870..4216)
                     /locus_tag="PKK81_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNV33395.1"
                     /translation="MAEDQRADLEACLAQARRSERRLRAQYAVTRVLAESSTLKDAGH
                     EILRAIGESLDWELGMFWAVDKQAELLRFVDLWHAPQVEASEFIQDSRERTFQRGVGL
                     IGRTWESGTPIWIPDVTSDPHFRRASIAAHVGLHGGFAFPVRKGERIDGVIEFFSHQI
                     RKPDQDILDMVADIGIKVGQFVDREQTAEALRQAEALADVARLLGDIGHDIKNMLMPI
                     MSGAALLEEELDECYHRLPESVTGNLKHSRDLTKELLDMIRNGSRRIQDRVREMAESV
                     KGLTRLPEFAPCRLAEVVAGVYAALRILADQRKVALRVDGLDTLPVIQADESRLFNAI
                     YNLVNNAIPEVSPGGSVTLRGRTDQAGRSILVSVIDTGRGMPAEVLKSLFTYSAISRK
                     VGGTGLGTKIVKDVVDAHGGSITVESEPGVGTSFHITLPLEGPPPHSQKASTNSPR"
     gene            4671..4985
                     /locus_tag="PKK81_10710"
     CDS             4671..4985
                     /locus_tag="PKK81_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33396.1"
                     /translation="MKAVTTSASTSMAARPLRRDRHWLALTGPSGFAARLFRVNADRW
                     RRRALFYERHRDYFPRLASGRFVERCRELEAAYRGLAHVLFTLQPQIEWAPVRAVVSR
                     IK"
     gene            5257..>5956
                     /locus_tag="PKK81_10715"
     CDS             5257..>5956
                     /locus_tag="PKK81_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV33397.1"
                     /translation="MRLMTILLTLSVLGASGCAKSTQPARNNRTQQAFAESMPPGHLF
                     QRHMIDLNGLTLSVLEAGNGDPVIFVHGVVTTSNIFPQYVSAFSPDFRGIAVDLRGYG
                     DSEKPPSGFTIEQFSKDLITLADTLKIDRAVWVGVSMGGMILQRLALDHPERVRALVL
                     VSTTDGAMILDDDIPTIGAPRDYREVSKDMIVESFPPDAPAQTYRPLLERIPTWNATV
                     IREALTSMSQFNVHG"
BASE COUNT         1115 a         1745 c         1837 g         1259 t
ORIGIN      
        1 agcccaagga acttgcggcc gccattacgg ttgaggcgag cgaattgctg gagatcttcc
       61 aatggaagtc gcacgacgag gtggcccgtc tcctggaaag cccgtcacgg gaacgcgttg
      121 aggatgaaat cgccgatgtc gtgatcctgc tttcctacct gtgtcatgat ctgggcctgg
      181 atgtgaatgc cgcggtgttg tcgaaactga agaagaacga agccaagtat ccagtcgaga
      241 agtcctatgg caacgccagg aagtatgacg aatgatcgag cctcttgatc tgttccaggg
      301 aggtatcatg atgttgcgat ctgtcttgct attcgtggcg gtctcggtcg cgttcgccgg
      361 ctgcggagcg accaggcatg cccggtcggt ggagccgtcc ggatttctgc aggatctcta
      421 tgcgcagatg cgcgagggca agggcgacga agcgctcctg gtgtatcgca atccggcagt
      481 cgattggccg gcgaaggctg cgtacaacaa gattctctta gatccgatca tgatctggcg
      541 ggggaaagac tccaaggtcg atgggctgga tccgaaggag gcccaggtga ttgctggttc
      601 cttctacgcg ctcctgtatc aggaactcgc caaggactac gagatggtga gcgaaccggg
      661 ccccaagatg tttcggatgc aggccgcgca caccgatgcc gaacaatcct atccagcctt
      721 ggatatcgtc tcgagtgtgc cggcgccgtt taacatcgcc ttcgtcgcct cgaccatcaa
      781 gacgctcacc accggaaaac cgttgttcaa aggcacggcc tccatcgagg gcaaactcat
      841 ggatgcggag tctggggaga tcctcgcggc cgccgtggat cggcgggtcg gcgggcgctt
      901 tctcgacgcg gaggtcttcg attcctggaa cgacgtgcac ggcgccttga agtactggtc
      961 gcagttgacc cggtttcgct tgtgtcagtt gcgcaagcaa accaactgtg tttcgcccgg
     1021 ctcgtaggtc gatcttttcg atctctgcgc accgatatag atcctttggg ccttgagcga
     1081 ccagcgggag ttcagtggga gaccgaggaa cgcggccagg taacgcatgg ttgagagggc
     1141 atggaggtga cacaagcgag gcgagagtct tcttgacgca tccgtccggt cggctgcctt
     1201 caaacacctc gctcagttga agcgttttag ccgaccaggt accatgccgc ctcatcttgt
     1261 gtggaggcac tatggcgcag attaccgaag ttgctccgga cctgttccgc atcaccacct
     1321 tcctcgaacc gttcaacctg caattcagtc agtttctggt ccgtgatgcc gagccgctcc
     1381 tgtatcacac cggcccgcgc gcgctgtttc cggcggtgaa ggagacggtg gcgtagctca
     1441 tcgacgtccg caccttgcgc tggatcgggt tcagccattt cgaggccgat gagtgcgagt
     1501 cgttgccgga gtggcagcag ttggcgcccc agtccgatgc ggtctgtagt ctggtgagca
     1561 agctcgtgag tgtggatgat tgtctcgcgc tccggccggc gaaggggatg acagatggcg
     1621 aggtgctgga gacggggaag taccgctttc gatttctggc cacgccgcac gtgccgcatt
     1681 gctgggagtc cggtctgatg ttcgaagaaa cacagcggac gctgctctgc tccgatcttc
     1741 tgcatcaaaa cggcaatgtg gaaccggtca cccagtcgga cgtggtgggc cgcagtcggg
     1801 acgtgctcgt ggaatatcag cagggtccac tggcgaacta catgccctat tgcacgctga
     1861 cagatccaac attgcagcgg ctggcggcgc tgcagccgaa gaccgtggcg acgatgcacg
     1921 gctcgctcta tgtcggcgac gggggaaaag cgctgcgtga tttggccacg gtctttcgag
     1981 aggtgttggg cgctgcctga cacgttgagg cgggaaagcg gccggggggg cctgcttgtt
     2041 gaccggtgga gtttcaatcg gctaggctcc tcgcgttcct gctgcatgtc tgccgctccc
     2101 gaaacgatcc gttgcgaggt gttatgcgca tacgtcacgt cgtcattgcg ttcagcctgg
     2161 ccggttgcct gaccataccc gggtggggcg tgatcagggc cgaaccctcc tttgatcaga
     2221 cggccgggta catcctcaat attgtcagag cctttcgtac cgcctacgtg ctccacgtcg
     2281 tcgaacatgc gcgagacgcg ggactctcgc cgcgagagga ttggaaaaca gatgcccatt
     2341 ttctcccgct tcctgcccag ttcgtcaagg aagccgctga acaggtggag ggtttggaga
     2401 tcggcctcat cagtttgacg ccgctgaatc cggcgaaccg cccccgcacg gacgcggaga
     2461 tgactgcgtt acttcagttg gagaaggatc gacaacgcgg ggtcatcggc ttcgtcgacg
     2521 gcgatgaatt caaggcggtg tcggccgatt tggcgctggt gcggtcctgc gtcgattgtc
     2581 acaaccagca tccccgtgcg gtgcgcaaaa actttcagca atgggatgtg atgggcgccc
     2641 tggtggtccg gctcaagcgc acggtcgaag gggaaggtca ggccctccct ccggaacctc
     2701 ctaagcgggc accaggactg ctcgaggggc ctccgcctcc gccgacgatt acgccgccct
     2761 gggttcgatg accggctgat cgggcccacg catctggaag agagcgggcc tggttgccgg
     2821 agtcagtctc tgttagcgat gatctgcgga catcgatccc atggcctggt catcgcggac
     2881 tgttcgtcga agccttctgc gagtggggcg gtggtccctc caggggaagc gtgatgtgaa
     2941 acgaggtgcc gacgcctggc tcgctctcaa cggtgatgct gcctccatgg gcatcgacga
     3001 catctttcac aatctttgtg cccagccctg tcccgccgac ctttcggctg atggcgctgt
     3061 acgtgaagag gctcttgagg acttcggctg gcatgcccct accggtatcg atgaccgaca
     3121 ccaggatgct ccttcccgcc tgatccgtgc ggccacgcag cgtgacggat ccgccgggtg
     3181 acacttccgg aatcgcgttg ttgacgagat tatagatcgc attgaacagc cggctctcgt
     3241 cagcctgaat cacggggagg gtatcgagtc catccacgcg cagcgcgacc ttacgttgat
     3301 cggccaggat cctgagcgct gcgtacacgc cggcgaccac ctccgcgagt cggcagggcg
     3361 caaattccgg cagccgggtg aggcctttga ccgattccgc catctccctg acccggtcct
     3421 gaatgcggcg ggacccattc cggatcatat ccaggagttc cttggtcaga tcccgactgt
     3481 gcttcagatt gccggtgaca ctctccggta ggcggtgata acactcgtcg agttcttcct
     3541 ccagcagcgc cgccccgctc atgatcggca tcagcatatt cttgatatcg tggccgatgt
     3601 ctcccagcag tctcgccacg tcggccaggg cctccgcttg gcgcagcgct tccgctgtct
     3661 gctcgcggtc cacaaactgg cccactttga tgccgatgtc ggccaccatg tcgagaatat
     3721 cctgatccgg cttgcggatc tgatggctga aaaattcaat gactccgtca atgcgttcgc
     3781 ccttacggac tggaaaggca aatcctccat gcagtccgac gtgagccgcg atcgaggccc
     3841 ggcggaaatg ggggtcactg gtcacatcag gaatccaaat aggcgttccg ctttcccagg
     3901 ttcgaccgat gagccccaca ccccgttgaa aggtccgttc ccggctgtcc tgtatgaact
     3961 cggaggcctc aacctgcggc gcatgccaca agtcaacgaa acgcagcaac tcagcctgct
     4021 tgtctacggc ccaaaacatg cccaattccc agtccagact ttcaccgata gcccgaagga
     4081 tttcatggcc tgcgtctttc agggttgagg actcggccag cacacgggtg actgcgtatt
     4141 gggcacgaag ccgacgttct gaccgccgcg cttgagccaa gcaggcctct aggtcagctc
     4201 tctggtcttc agccatgttc cctccctgtg cagatccttg catccagtgt gtgaacccac
     4261 cggattggag cacgcgctat caggattatg atcgcgtccg gaaagacttt cacggggaga
     4321 ttgtagctga atccgccatc gcctgccttc cttcccacag tcgcggcagg cattggacct
     4381 gcgccctgtc tgagggcagg cgcgggatct gtcacagcca cgtccgtaga gactgggccc
     4441 tccgaacgga cagacccata ggatttcgag tcccggaatt gtgaaactgc cgtagccgga
     4501 agccccggcc agctgtgctt gtatgaagtc tttctcatgc tgcactaatc atctcctcat
     4561 gtggcgagac tacagtaggg gcagggtaaa ccgagactgg tgtcatcaca gcgcctctgt
     4621 caggacgttc gaatctatga cacaggcgaa cggaagaagg aggagaaccg atgaaagcag
     4681 tgaccacatc cgcatccacc tcaatggctg cacgtcctct cagacgggac cggcactggt
     4741 tggcactcac cggtccctcc gggtttgccg cccggttgtt cagggtcaat gccgatcgct
     4801 ggagacggcg agcgctcttc tacgagcgtc accgcgatta ctttccgcgg ctggcgtccg
     4861 gtcgattcgt cgagcgctgt cgtgaattgg aggcggcgta ccgggggctt gcgcacgtcc
     4921 tgttcactct gcaaccgcag atcgaatggg cgccggtccg ggcggtcgtc tcgcgaatca
     4981 agtaagttcg gatgatcgcg cggtcggcat gttgtctgcg tgacccacca gcgcggcgct
     5041 atcggccgtc gatctgaggg gcccgacgat gggccggatg gcatgcctgt gttcctgtgc
     5101 gcagggcaac cactcagtgc cgggagtcat caattcgagg agtccaagaa attgtgcggg
     5161 agcgagaatg ccgggtggca gctcagcatt gcatctgctc gcctgacagc ttaggctgca
     5221 tgtagattca gtgatctctg tagaaagggg agttgcatgc ggttgatgac gatcctgctc
     5281 acgttgagtg ttctcggcgc gtccggctgt gcgaaatcca ctcagcccgc tcgtaacaac
     5341 cgcacccagc aggcctttgc tgagtccatg cccccgggtc atctgttcca acgccatatg
     5401 atcgatctca atggcttaac cctcagcgtg ctggaggcag gcaatggcga tccggttatc
     5461 tttgtccacg gcgtggtgac gaccagcaat atctttcccc aatatgtgag tgccttttcg
     5521 ccggatttca gagggattgc ggtggacctg cgtggatacg gcgattcgga gaaaccgccg
     5581 agcgggttca cgatcgaaca gttttcgaag gatctcatca ccctggccga tacgctcaag
     5641 atcgaccgtg ctgtgtgggt gggtgtgtcc atgggcggga tgattctgca gcgtctcgcg
     5701 ctggatcatc cagagcgggt ccgggcgctg gtgctggtgt ccacgaccga cggggcgatg
     5761 attctcgacg acgacattcc gaccatcggc gcgccgcgtg actatcggga agtgtcgaag
     5821 gatatgatcg tggaaagttt tcctcccgat gcgccggctc aaacctacag gcccttgctt
     5881 gaacggatcc ccacctggaa tgccacggtg atccgggagg cgctgacttc catgtcgcag
     5941 ttcaacgtgc atggcc
//
LOCUS       DAOQRM010000148         5904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_13657_length_5904_cov_13.040520, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000148 DAOQRM010000000
VERSION     DAOQRM010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13657_length_5904_cov_13.040520"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1353
                     /locus_tag="PKK81_10720"
     CDS             <1..>1353
                     /locus_tag="PKK81_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33398.1"
                     /translation="VYQSKKLIMRASMSSELNVLGHQLNRLSERDRHYRDFTLNSLTH
                     AVREIIACFPVYRSYLTTDREAPLDRDQAYIVLAVARAKRRNPTLNGQIFDFVRDLLL
                     GKLDPSTGLTKEDQIRFVTKFQQTTGPVMAKGVEDTAFYVYNRLISLNEVGGDPAHFG
                     SSVEAFHQAIRERRAGWPYSMSATSTHDTKRGEDVRARINVLPELRERWSKAIARWAR
                     LNRRYRTEVEERPAPDRNDEYLFYQTLVGAWPLMTMDEVRYEEFVTRIERYMIKAVRE
                     AKTHTSWINPHPDYEAALCRFIRVILSSREPNPFLDDFLPFQAVVARYGMYNGLSQLL
                     VKVAAPGVPDCYQGAELWELNLVDPDNRRPVEYALRARMLKEFDGAATNEAGDRIEFL
                     HRLVESWQDGRLKLFILQAALRHRRAYPDLYLHGDYVPLDCEGPHQFHLCAFARLHQD
                     Q"
     assembly_gap    1354..1453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1569..1928
                     /locus_tag="PKK81_10725"
     CDS             1569..1928
                     /locus_tag="PKK81_10725"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNV33399.1"
                     /translation="MALHTGRRLLAASTIEGTPVQNQAGEDLGEINELMIDLEKGRIA
                     YAVLSFGGFLGLGDKLFALPWEALTMSAGGDFFILNVTRERLEQAEGFDKDRWPDMAD
                     TAWGERLHTYYGYKPYW"
     gene            1984..2691
                     /locus_tag="PKK81_10730"
     CDS             1984..2691
                     /locus_tag="PKK81_10730"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNV33400.1"
                     /translation="MMRQIAIYAGTIALLLATSSPGFSQGSREGGTSDSTMGKGAGTS
                     TPRETINPDKKTDLTGGKQGVPEEYADTPVRQGKLEEEKGSKYANASVYNTQGAEIGK
                     IHQVLKDTKTGDIEYVVFVPNESKRPIPIRWSQFVTKGDKLQLNLKKEDIQNPVSMNS
                     SKDMSPDIKHYMDKMEQVREAPSAPGNPGIPGQKGPGATGPMGEESVGGGGPSGTSGL
                     PPGQAPGFEGGHPSSKR"
     gene            2834..4117
                     /locus_tag="PKK81_10735"
     CDS             2834..4117
                     /locus_tag="PKK81_10735"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV33401.1"
                     /translation="MQRVVIVGGGFGGLAAARALEGAAVQVVLVDRVNHHLFQPLLYQ
                     VATAALSPADIAWPLRTLFRGQQNIRITLGEVTKIDRAARCVLLSETAPIPYDRLILA
                     PGARHAYFGHEDWEMHAPGLKTIPDALELRERMLMAFEQAQQAGDAQRASDYLTFVIV
                     GGGPTGVELAGALMEIGRRAMAPDFPVLHRAEFRVILIEGGGRILEAFPPDLSGKAQT
                     ALEALGVTVMVNCRVHDVTGQGVLAGAQFIRTANVLWAAGNIASPILRSLDVPLDQTG
                     RVRVERDLSLPGDPWTFVIGDAAHCESPEGRALPGVAPVAMQEGRYVAGLIAHDIRKE
                     HRRFFRYRDRGMLATIGRAKAVARFGPVSSSGLVAWLAWSFIHIFFLIGFRNRFRVMV
                     EWIWYYITFKPGARLIYGKPRGLREEGAGEANGLP"
     gene            complement(4128..4910)
                     /locus_tag="PKK81_10740"
     CDS             complement(4128..4910)
                     /locus_tag="PKK81_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV33402.1"
                     /translation="MSARGTFVSILAWPLKGLVRTILSPSLYQQVRVTFLGEQSGLGL
                     DWRWANSLRRPHPIRRDFGWQAGQPIDRYYTEQHFLPACRADIRGRVLEIGDDRYTRQ
                     FGAEKVTVSEVLHLHPDTPGATICADLTKADHIPSDSFDCIILTFTLQFIFDVRAAIR
                     TVARILKPGGVALLVFPGITQISRYDMDNWGEFWRFTSLSARRLFEEVFRPDDVTVTT
                     YGNVLAATASLQGLISSELRREELDHWDRDYELVIGLRAVKR"
     gene            complement(5410..5763)
                     /locus_tag="PKK81_10745"
     CDS             complement(5410..5763)
                     /locus_tag="PKK81_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33403.1"
                     /translation="MKRIVEKKMQARKKSSSKVRRVKQREIEPSTHVIERKPRRASGV
                     ATELKKQRAKKTVALTKKASTSRGQAKKVGRAVGKILGRVIGTVEQTVAKVMPGSKPT
                     AARQTPSSGRRKRRS"
BASE COUNT         1181 a         1591 c         1808 g         1224 t
ORIGIN      
        1 gtgtaccagt ccaagaaact gatcatgcgg gcctccatgt ccagcgaact caatgtgctc
       61 ggccaccaat tgaatcgact gtccgagcgg gaccggcact accgggactt taccctcaac
      121 agcctgaccc atgccgtccg ggaaatcatt gcctgttttc ccgtctatcg ctcgtatctg
      181 acgaccgacc gcgaggctcc gcttgaccgc gatcaggcct atatcgtgct ggctgtcgcc
      241 cgggccaaac ggcgcaatcc aacactgaac ggtcagatat tcgacttcgt ccgcgacctg
      301 ctcttgggaa agctggaccc gtccacagga ttgacgaagg aggatcagat ccggttcgtc
      361 acgaagtttc agcagacgac aggtccggtg atggccaagg gcgtcgagga cacggccttc
      421 tatgtctaca accgactgat ctcgctcaat gaggtggggg gagatccggc tcatttcggc
      481 tcctccgtgg aggcgtttca ccaggccatt cgggaacgcc gcgccggctg gccctactcg
      541 atgtcggcaa cttcaaccca tgacacgaaa cgtggcgaag atgtgcgcgc tcgaatcaat
      601 gtgctcccgg aacttcgaga gcgttggagc aaggccatcg cccgctgggc caggctcaat
      661 aggcggtacc ggaccgaagt cgaggagagg cctgctcccg atcgtaatga cgaatatctt
      721 ttctatcaga cactggtagg ggcctggccg ctcatgacga tggacgaggt ccggtacgaa
      781 gagtttgtga cgcgcatcga gcggtacatg atcaaggcag ttcgggaagc gaagacgcac
      841 accagttgga tcaatcccca cccggactat gaagccgcgc tgtgccggtt cattcgcgtc
      901 atcttgtcat cgcgggaacc caatccgttt ctggacgact ttctgccgtt tcaggcggtg
      961 gtcgctcgat atggcatgta caacggcttg tcgcagttgt tggtgaaggt ggccgccccc
     1021 ggcgttccgg actgttacca gggcgcagaa ttatgggaat tgaatcttgt cgatccggac
     1081 aaccgccgtc cggtggagta cgcgcttcgc gccaggatgt tgaaggagtt cgatggagcc
     1141 gcaacaaacg aggccggcga tcgtatcgag tttctccatc gtctcgtgga atcctggcag
     1201 gacgggcgac tgaaactatt tatcttgcag gcggcactgc gccacaggcg agcgtatccg
     1261 gacctgtatc tgcatggtga ttatgttcca ctcgactgcg agggccctca ccagttccac
     1321 ctctgcgcat tcgcgcgatt gcaccaggat caannnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnagaattt ctaggggcct tcctagctgt tcctctcgac ctcgggcgtt
     1501 atgactaaga gtacgaggtc gatttgtctg cctcagaaca tcgcatatca acacaaggag
     1561 ggtttctcat ggcgttacat acagggcgac gattgctggc ggcttctacg atagagggca
     1621 cgcccgtgca gaatcaggct ggagaagatc ttggagagat caatgaactg atgatcgatc
     1681 tcgaaaaggg acgcattgct tatgcggtgc tctcgttcgg cggctttctt gggttgggcg
     1741 acaaactgtt cgcgcttcct tgggaggcat tgacgatgtc cgcaggcgga gatttcttca
     1801 ttttgaacgt gaccagggaa cggctggagc aagccgaagg gtttgataag gatcgctggc
     1861 cggatatggc ggataccgcc tggggtgagc gcctccacac ctactatggc tacaagccgt
     1921 actggtgaga cgtttatctg aattacatgg ccatcggtag cacacctgca aaaaggagaa
     1981 ctgatgatgc gacaaatagc catttacgcc ggaacgattg ctctcttact cgccacgagt
     2041 tcgccggggt tctcacaagg atccagggag ggcgggacgt ccgattccac tatggggaag
     2101 ggtgcaggaa cgtccacccc gagagaaacg atcaatcccg acaagaaaac agatctgacc
     2161 ggtggaaaac agggagtgcc tgaagaatat gcggatacac cggttcgaca gggaaaactc
     2221 gaagaggaga aagggagcaa atacgccaat gcttctgtgt acaatacaca gggcgccgag
     2281 atcggcaaga ttcatcaagt cttgaaggac acgaagaccg gtgacatcga atacgtcgtg
     2341 ttcgtgccca acgaatcgaa gcggccgatt cccattcgct ggagtcagtt tgtgacgaag
     2401 ggcgacaaac ttcagttgaa tctcaagaaa gaagacattc aaaatccggt gtccatgaac
     2461 agcagcaagg acatgtcgcc ggatatcaag cactatatgg ataagatgga acaggttcgc
     2521 gaagcgccct ccgctcccgg taatcccggc attccagggc agaaggggcc gggtgcaacc
     2581 gggccgatgg gtgaagaaag cgtgggcggt ggtggcccga gtggaaccag tggattacct
     2641 cccggacagg cccctggatt cgaaggcggc catccgagca gcaagcgcta gttcgacacc
     2701 ttgtgtgaag tgatacaggg caggctcttg tgagagagcc tgccctttcc tttttgggat
     2761 agagtggcat gatacacatg gttccgggca gctgatatgc ggatatgcca gcctggcaag
     2821 gagggagcgc gcgatgcagc gtgtggtgat cgtcggggga gggttcgggg gcttggctgc
     2881 tgccagagcg ctcgaagggg cggcggttca agttgtactc gtggatcgcg tcaatcacca
     2941 tctctttcag ccgctgctct accaggtcgc gactgctgcg ttgtcaccgg ctgatatcgc
     3001 ctggccgctc cggacgctgt ttcgaggaca gcagaacatc cggattacgc tgggggaggt
     3061 caccaagatc gatcgagcgg cgcgttgcgt cctgctctcc gagactgcgc cgattcccta
     3121 tgaccgactg atcctggcgc ctggtgcgcg acatgcctac tttggccatg aagattggga
     3181 gatgcatgcc ccggggctga aaaccattcc cgatgccttg gagcttcgcg agcggatgct
     3241 gatggccttt gagcaggccc aacaggcggg tgacgcgcag cgtgcgagcg actatctcac
     3301 cttcgtcatt gtcggaggcg ggccaaccgg agtcgagctg gccggcgcgc ttatggagat
     3361 cggacggagg gcgatggcgc cggattttcc ggtgttgcat cgggcggagt ttcgggtgat
     3421 cttaatcgag ggtggaggcc gcatactcga ggctttcccg cctgatttgt ccgggaaggc
     3481 gcagacggca ttggaggcgc tcggggtcac cgtcatggtg aactgccggg tccatgacgt
     3541 aaccgggcag ggggtcttgg ccggcgcgca attcattcga accgccaatg tgctctgggc
     3601 agcagggaac atcgcgtcgc cgatattgcg atcgttggat gtgccgttgg atcagaccgg
     3661 gcgcgtccgc gtcgagcgag atctcagtct gccgggtgat ccgtggacgt ttgtcatcgg
     3721 cgatgccgcg cattgcgagt ctcccgaagg gcgcgctctg ccgggcgtgg cgccggtcgc
     3781 gatgcaggaa ggtcgctatg tagccgggtt gattgcccac gacatccgga aggagcatcg
     3841 ccggttcttt cgctacaggg atcgtggcat gttagccacc atcggcagag ccaaggccgt
     3901 ggcccgattc ggacctgtgt cgtcttcggg cctggtcgcc tggctggcct ggtcttttat
     3961 ccatatattc tttttgatcg gcttccggaa ccggtttcgg gtgatggtgg aatggatctg
     4021 gtactacatc acgttcaagc ccggtgcacg attgatctat gggaagccgc ggggattgcg
     4081 cgaagaggga gccggggagg cgaatgggct gccctgagcc aggagtccta tcgcttcacc
     4141 gcacgcaggc cgatcaccaa ttcataatcc cggtcccaat ggtccaattc ctcgcgacgc
     4201 aattccgagg agatcagtcc ttgcaagctt gctgtcgcgg ctaagacatt gccataggtg
     4261 gtgaccgtca catcgtcggg ccgaaaaact tcttcgaaca gccttcgtgc cgacaaggag
     4321 gtgaagcgcc agaactcccc ccaattgtcc atgtcatagc gggagatctg ggtgataccg
     4381 ggaaacacca gcagggcgac gcccccgggt ttgagaatgc gtgccacagt cctgatcgcc
     4441 gcccgcacat cgaagataaa ttgcagggtg aaggtgagaa taatgcaatc gaaactgtcg
     4501 gaggggatgt gatccgcttt ggtcagatcc gcgcagatcg tggcgccggg agtgtcgggg
     4561 tgaagatgga ggacctccga caccgtgacc ttctccgcac cgaattgccg ggtatatcga
     4621 tcgtctccga tttccaacac cctgccgcga atatccgcgc gacaggccgg tagaaagtgc
     4681 tgctcggtat aataccgatc gatcggttgt cccgcctgcc aaccgaagtc ccgacggata
     4741 ggatgcgggc ggcgcagaga atttgcccac cgccagtcga gtcccaatcc tgactgttca
     4801 ccgaggaacg tgactcgcac ttgctgatag agggaagggc tcaggatcgt cctgacgagt
     4861 cccttcaacg gccatgcgag gatcgacacg aaggttccgc gtgcactcat gggcacacct
     4921 cggcgacgtg gtgattccgt aagaggcgtc gagtcccgga gtgaagggtt caacatagca
     4981 tgctctcttt cgtcatggaa gatagcctac ctggtcctag ctggaggatt tccagatgac
     5041 ggtgatggcg ggggacccgg acgtggaggc ttctcggggt cgatcgccat cttagggtcg
     5101 acgaccggtg gaggcacgac ggcttcgggc gccggcggag gcagatgctc cggttgtttg
     5161 aggatcccag ggtcagtttg ggatggcggc gccgggggcg ccaggccagg cttgggcaaa
     5221 gggggagcgg actcagcagg cggcaacgca ggcccggggt cggcagtggc cacacttgca
     5281 gccggcagat aaaggagaag cgccgcgata agaagctgtg ccgatgtttt tctcatggct
     5341 gatcctcccc ataattcgag cggatcgctg tgaggctagt ggtccgctca tcctgacagc
     5401 ccgggactct catgaacgcc gtttgcgccg accgcttgat ggggtctggc gtgccgctgt
     5461 tggcttcgac cctggcatca ccttcgccac tgtttgttcg actgtgccga tcactctgcc
     5521 cagaatcttt ccgacggcgc ggcccacctt tttggcctgt ccgcgtgagg tcgaagcctt
     5581 tttggtcagc gcaacggttt tcttggccct ctgcttcttg agttctgtgg ccacgccgga
     5641 ggctcgtcgt ggcttccgtt cgatgacatg agtggagggc tcgatctcgc gctgtttgac
     5701 gcgtcgcacc ttcgaactgc tcttcttgcg ggcctgcatt tttttctcaa cgattctctt
     5761 catgttccct ccctatgatc tagtgcccat cctttaccct gggtgatatc tgctcgatga
     5821 tccaggtcgg accgtcgggg tacaaactac cttcaaagcg gacaaacgcc ggcgcgtcct
     5881 tcttaatcag ccaaaaatga tatt
//
LOCUS       DAOQRM010000149         5883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_13732_length_5883_cov_15.635724, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000149 DAOQRM010000000
VERSION     DAOQRM010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13732_length_5883_cov_15.635724"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PKK81_10750"
     CDS             complement(<1..821)
                     /locus_tag="PKK81_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33404.1"
                     /translation="MASIKPTESAILNPGDPIRPEKPAPSKLLAGVVLVGGFIVLSMV
                     LQQFRGNQPGIVRGKSPAKTVSINREETDKEIYMKDAGARIEALEQQRDQMRRLLEQD
                     GRDKTEIKTQLSDLQKRIESVARSGGVVSAGTKSGALPPLPPPLSANPVGGGSATPVS
                     AMPVPKGPIPVQSTPKPAGGTLPAPGAGGGLNEGFQVFRPEIVPGAKKNEKKLWLNTG
                     TIIPVKLLSGMDAPARSTGAQGGGASANSPYPVLMMVRDIMKLPNEARIDAKDCFL"
     gene            complement(842..1615)
                     /locus_tag="PKK81_10755"
     CDS             complement(842..1615)
                     /locus_tag="PKK81_10755"
                     /inference="COORDINATES: protein motif:HMM:NF018311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HNV33405.1"
                     /translation="MTSSVYRDSKHRAMAMCVLGLLLFLSWAKSVGAAVVVGDEGATS
                     LTLSGRDLNVLTFPAPITMATSSRADLKAKPEGRNLIVQVKTGPADLVVMAGEQTYVF
                     EVGVSVSLGAQTISIEDRRVGTVVYDTDPARQAADYVDGLLETLQMAARGALPKGYRQ
                     TSIAEERYPKWLELKVVQGIEYRGPKYRVIVYRLVNTAGHKYTLREQEFLTGVQKAIA
                     LNREVVESGEEVVVYLLDDAPPEPPKPKPEPKVDPDAKS"
     gene            complement(1626..2207)
                     /locus_tag="PKK81_10760"
     CDS             complement(1626..2207)
                     /locus_tag="PKK81_10760"
                     /inference="COORDINATES: protein motif:HMM:NF017152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HNV33406.1"
                     /translation="MTIELWKSKLHNAQVLNLVFAALATVLAVALVANGFFRQNVAVH
                     VVPINPTQEYWANAMGGDEYYKKSIAMAMLPWIANVTPASVEFHHRQLLQWVPSSHYG
                     LMSESLGAEAVYVKTNNLSRVFFVTASRVDGDVAVFEGIERRFVGKSEIREEEREYRI
                     SMRWDQGRPWVVGLSAAHLDRSRQDSQSDPAKG"
     gene            complement(2213..2494)
                     /gene="traL"
                     /locus_tag="PKK81_10765"
     CDS             complement(2213..2494)
                     /gene="traL"
                     /locus_tag="PKK81_10765"
                     /inference="COORDINATES: protein motif:HMM:NF018836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HNV33407.1"
                     /translation="MNGTFIPRYLDSLPQIFWWEIDELAVLFVCIFLGIVTKFLTYLI
                     VVGIVSTLILSKMKNGKSEGFLFHWAYWSGVPTFQLTGSPHGTTREMME"
     gene            complement(2573..2893)
                     /locus_tag="PKK81_10770"
     CDS             complement(2573..2893)
                     /locus_tag="PKK81_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33408.1"
                     /translation="MGNVLKRVTGEMWRMSLLLGLMVLASFVVLAVPAFAGADATFGP
                     LVTLLTNYMQGSLGTLLALVAGAIGLGSAMAGRWAGMFTGFGVAAAAFYIPNIIPTIT
                     TGLM"
     gene            complement(4226..5014)
                     /locus_tag="PKK81_10775"
     CDS             complement(4226..5014)
                     /locus_tag="PKK81_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33409.1"
                     /translation="MPSSAVGGLMLEYAADWQESLVQRAEQCARSITYVAGELIRERF
                     RLGGIVVEALERSQHGDAVVVKMAERMSAAVGKCIRPHMLYESARIYNAFGGQWERIE
                     ALRAQLSFPLSYSYLVRFCLPALTMDSAWNAEQVTYSNDSELRGFESAVLRIEARVQA
                     HRTRQQEGGKERSTDLETEGFVGACTQSAEFQEMAVRTIVRRLDVLCEALEGKAQQRG
                     VELEALRESQEAIARIASRMATLGTLIHAPVMHAVIPDNNTSVL"
     gene            complement(5004..5816)
                     /locus_tag="PKK81_10780"
     CDS             complement(5004..5816)
                     /locus_tag="PKK81_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33410.1"
                     /translation="MRGGRKWLLAVAGALGLVCLGGNVVMALSPANISPGYPTSLDDA
                     YPVPTDQVVIQSAIRPDITRNDSGSESGRFRATHDIRYGATENMELTVSGTSLRGPLN
                     PGTMDDPRSIQLGILARFRKQAEERELLPSISVRVLGEVPYAGDRTNPGLRGALVTSW
                     NLGNRWWLHGNLGYEVVPSFQPGHWVPHRSSIILVNLGFVKALDESSAIVGGLRFQED
                     PLNSKKQVASPELGYVLALDKHWLFSLSASRDFLGSVGQAAFRGSVGVIYAF"
BASE COUNT         1285 a         1946 c         1430 g         1222 t
ORIGIN      
        1 aggaaacagt ccttcgcatc aatcctcgcc tcattcggga gcttcatgat gtcacgcacc
       61 atcatcagca ccgggtaggg actgtttgcg gaggcgcccc caccctgcgc ccccgtcgaa
      121 cgggccggtg catccatgcc agaaagcaat ttgacgggaa taatcgtccc cgtattcagc
      181 cacaatttct tctcgttctt tttggcgcca ggcactatct ctggccgaaa tacctgaaac
      241 ccctcgttca acccgccccc ggccccaggg gccggtagcg tcccgcctgc cggtttcggg
      301 gtgctttgca ccggaatggg gcctttcgga accggcatgg ccgagacagg cgtcgcactt
      361 cctccaccca cgggattggc actgagcgga ggcggcagcg gcggcaacgc gccactcttc
      421 gtcccggctg agaccacgcc accagaccgc gccactgact caatccgctt ctgcaggtca
      481 ctcaactgag tcttaatctc ggtcttgtct cgtccgtcct gctccaacaa tctccgcatt
      541 tgatcccgct gctgctccaa cgcttcgatc ctggccccgg cgtctttcat ataaatttct
      601 ttgtcggttt cctcgcgatt gatcgacacg gtcttggctg gagacttccc acggacaatc
      661 cccggttgat tccctcggaa ttgctgaagc accatgctca gcacgatgaa cccacccacc
      721 agaaccactc ccgcgagcag tttggatgga gcgggtttct ctggacgaat cgggtcgcca
      781 gggttgagaa tcgcgctctc cgtcggttta atcgatgcca tcctaggtct ccggtctgag
      841 gttagctttt tgcgtctgga tccactttcg gctcgggctt gggtttgggc ggttcaggcg
      901 gtgcgtcatc cagcaggtac accaccactt cttcccctga ctcgaccacc tcgcgattta
      961 aggcgattgc tttttgaacc ccggtcagga attcttgttc ccgcagtgtg tacttgtgtc
     1021 ccgcggtatt caccaatcga tacacaatca cccggtactt cggccctcga tactcgattc
     1081 cctgcaccac cttcagctca agccacttcg gataccgttc ttcggcaatc gacgtctgcc
     1141 gatacccttt cggtaacgct ccccgcgcag ccatctgcaa agtctcgagt agcccatcca
     1201 catagtcagc ggcttggcga gcaggatccg tgtcatagac cacagtcccc acgcgcctat
     1261 cttcaatact aatggtctgc gcgcctaacg acacgctcac cccgacctca aagacgtagg
     1321 tttgctcgcc ggccatcacg accagatcag cggggccagt cttcacctgg acaatcaagt
     1381 tgcgcccttc cggcttcgct ttgagatccg ccctagagct cgtcgccatg gtaatcgggg
     1441 caggaaaggt caggacgttc aaatctcttc cagacaatgt gagggacgtc gcaccctcat
     1501 caccaaccac caccgcagct cctaccgact tggcccaaga cagaaacaac agcaggccaa
     1561 ggacgcacat cgccatcgcc cggtgcttcg agtctcgata cacgctggat gtcatacgtc
     1621 tccccctacc cctttgccgg atctgattga ctatcctgcc gtgaacgatc taaatgagcc
     1681 gcagagagcc caaccaccca cggcctgccc tgatcccacc gcatcgagat acggtactcc
     1741 cgctcttctt cccgtatctc gctctttccc acaaatcggc gctcaatgcc ctcgaacacg
     1801 gccacgtccc cgtccacacg actagccgtc acaaaaaaca cacgactgag attgttggtc
     1861 ttcacgtaca cggcctccgc cccgagagac tctgacataa gcccatagtg gcttgacggt
     1921 acccactgca gaagctgccg atggtgaaac tcaacagagg ccggcgtgac attggcaatc
     1981 cagggcaaca tggccatggc aatggacttt ttgtaatact cgtcgccgcc cattgcgttc
     2041 gcccaatatt cctgcgtcgg attgatcggc accacatgaa ccgcgacgtt ctgccgaaaa
     2101 aatccgttcg ccaccaacgc caccgctaat actgttgcca gggcggcgaa caccagattc
     2161 aagacctggg cgttatgcaa ttttgatttc cacagctcga ttgtcatcgt cgttactcca
     2221 tcatctctcg ggttgttccg tgaggtgatc ccgtcagctg aaacgtgggc acaccagacc
     2281 aataggccca atggaagagg aatccctcgg atttcccgtt cttcattttc gacaaaatca
     2341 acgtgctcac gatccccacc actatcaggt aggtcaggaa cttcgtgaca atgccgagaa
     2401 agatgcacac aaagagcacg gccaactcat caatctccca ccaaaagatc tggggcaggc
     2461 tatcgagata tcgtggaata aatgtgccgt tcatctgtcc ctcttctgct tcattgtgac
     2521 ggcggggcca tgatctgtcc cgccgtcacg acaccatgcc tgtaccgccc gcttacatca
     2581 gtcccgtggt aatggtcgga atgatgttgg ggatatagaa ggctgctgcc gccacaccga
     2641 atccggtgaa catgccggcc caccggcccg ccatcgccga tcccaacccg atcgcccccg
     2701 ccaccagggc taacaacgtc cccagcgacc cctgcatata gttggtcaac aacgtgacca
     2761 gcggaccaaa cgtcgcatcc gcacctgcga acgcgggcac ggccagaacc acaaacgagg
     2821 caagcaccat gagccccaac aagagactca ttcgccacat ttctccggtc actctcttca
     2881 agacgttgcc catactccag cccctcctct ggctcgtcgc tcactgttta caagacgtgt
     2941 ggttgccaaa ccgaatgagc gcccttagtt cattccagca tagccatgct cctcctcagc
     3001 ctctccttat ggttttactg agtcgcctgt gacaccagtg gacacaacag atgttactgg
     3061 aggtatttgg gatcgctcct caaccgatga gggatctcca cacctacctc gcagcagcat
     3121 cgtgatctcc acgcgtcgac tgtccgcatg gttcggcata aagcagcagc ctccgcggcc
     3181 gaccacttcc acctgagtcg agaccttcaa gccagcctgc aatgctttcg ccaccgcgtg
     3241 tgcccgagcc gtcccaagtt cttcgtttgt ctcccgagac cccgtcggat cggtgtatcc
     3301 ctccacccgc agatagaaga cctcctctgt ggacagttcc gttaagtctc gttgcgcacg
     3361 ctccggtaca gcagccgaac ccatggcaaa caaaacggtt cgatgccgca cctcaggatc
     3421 gcgacgacag gcggacgggg acgtccttcc tgtgaaacca ggaggtgctg gtttgtccac
     3481 gctgctcacc gcagcgcgag acatgtcacc ttgacggcct gccagcgcac ccgcagaaaa
     3541 cgtcggcaat gtctggagcc gatgcatcac cgccttaatc ggtttcggtg tgagatccgc
     3601 caccagcggt ttgttaggga cctgcgtcag gggtgttggc tctgggcatc cagcgggacg
     3661 agacagaagc cattgcggac cagattggac ggaaaccacc gtagtcggga aggtgtagcc
     3721 ttgcccgcag agtcgcgttg cctcggacat tgttggcgac gcagcaggtc gcgtcgtaca
     3781 gcccccgacg gtcagcagtc ccatacaaac gagaccggcc catggcatgt aagtcgtccg
     3841 tccccctgtc atactccccc ctttcaccac ggcttccgag tcgccaggtt catctttggt
     3901 attaccgact gccacggtgt atcgaacgga aacaggggtt gccggatatc ggaagggacg
     3961 tcgtcgacgc gacgacggaa ccaagcaaag acgccagcgg gttaagaagc gatcagatta
     4021 acagtagcca cccacccacc ccaggcccgg aaggtcccgt cgggacattg ctggaataaa
     4081 cgcgcgcact gaagacactg atgaaggtac ctatccatga attcgtcacc atcgtgtgaa
     4141 cgatacccaa acaactctcc gttacaacga ggacacggtt tcaacaaatt tgcatacagt
     4201 cccattgttt gctctcctcg cttcactaca agacactcgt attattatct gggataactg
     4261 catgcatcac cggtgcatgg atcagcgtgc cgagggtcgc cattctggag gcaatcctcg
     4321 cgattgcctc ctgcgactca cgcagcgcct ccaattcgac gcctcgctgc tgagcttttc
     4381 cttccagtgc ctcgcacaac acatcaagcc gccgcacaat ggttctgacg gccatctctt
     4441 gaaattctgc ggattgcgta caggccccca cgaacccttc ggtttcgaga tccgtcgatc
     4501 tttccttgcc accttcctgc tgccgcgtac gatgagcctg cacacgcgcc tcgatacgca
     4561 agaccgcact ctcgaagccc cgtaattcag agtcgttgga atacgtcacc tgttcggcat
     4621 tccaggcact atccatagtc aaggccggca gacagaatcg gacgagataa ctataggaaa
     4681 ggggaaagct caattgggcg cgcagggctt caatccgctc ccactgccct ccgaaggcgt
     4741 tgtagatcct cgcggactcg tacagcatgt gtggacgaat acacttcccg accgctgcgg
     4801 acatacgttc tgccatcttc acgacgacag catccccatg ctggctccgt tccagggctt
     4861 caaccactat accgccaagc cgaaagcgtt cgcggatcaa ctctcccgcc acatacgtga
     4921 tggacctcgc acactgttca gcccgttgca ccaacgattc ctgccaatca gccgcgtact
     4981 ccagcatcag ccctcctact gcgctagaag gcatagatca ctcccactga cccacgaaac
     5041 gccgcttgcc caacagatcc gaggaaatca cgggaggcac tcaacgagaa caaccagtgt
     5101 ttgtccaacg ccaagacata ccccagctcc ggactagcga cttgcttctt ggaattgagc
     5161 gggtcttctt ggaatcgcaa gccacccaca atcgcagaag attcatccag ggcttttaca
     5221 aaccccaagt tcacaagaat aatgctcgaa cgatgcggga cccaatggcc tggctgaaat
     5281 gacggcacga cctcatatcc cagattgcca tggagccacc accgattccc aagattccag
     5341 gacgtgacca acgccccgcg caagccagga ttcgttcggt ctcccgcata gggcacctca
     5401 cccaatacgc gcacgctaat cgatggcagc agctctcgtt cttcggcctg tttccggaac
     5461 cgcgctaaaa ttcccaactg gatcgaccta ggatcgtcca tcgtcccagg attgagcgga
     5521 cctcggagcg acgtcccact gaccgtcaat tccatatttt ctgtggcgcc atagcgaata
     5581 tcgtgagtcg cccggaagcg cccactctca gagcccgaat cattccgcgt gatgtcgggc
     5641 cggatcgccg actggatcac cacttgatct gtgggcaccg ggtaggcgtc atcgagagac
     5701 gtagggtagc ccggactgat atttgccggg gagagtgcca tcaccacgtt gccccccagg
     5761 catacgagcc ccagggcccc cgcaaccgcc agtaaccact tcctgcctcc acgcattagt
     5821 cccaagctcc ttataggaat tgcacaacat agaaggttgt taccctcgta ttaccgaagg
     5881 acc
//
LOCUS       DAOQRM010000150         5860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_13805_length_5860_cov_21.018260, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000150 DAOQRM010000000
VERSION     DAOQRM010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13805_length_5860_cov_21.018260"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..>1288
                     /locus_tag="PKK81_10785"
     CDS             95..>1288
                     /locus_tag="PKK81_10785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV33411.1"
                     /translation="MKREATTDKGGVTLSTGHPQLLAEIRAEIAATGPIPFARFMDLA
                     LYHPKYGYYVRPVDDPTQERIGWSGDFYTSSDVHPILGQALARQAQQLDTLLGHPDPF
                     TVVEMGAGKGLLARDFLTACRNAPANLRDRLRYILIERSAAMRTQQQNNLAPWVGQAG
                     RVAWLDRLEDLPPNSVTGLFFSNELVDALPVHRLAVLDGRPQEVYVEYRDERFSEVYR
                     PLSNELTAYLREGGINLPDGYRAEINLDAVDWMTQVAQVMARGAVLTIDYGHTAEDLY
                     GPDRKNGTFLCYYHQTTSEDAYDRVGEQDMTAHVDFTALAQTGQQAGLDVTGFTNQMS
                     FLIGLGAEQLLESLQPESPEFYAAIHLLRPDGMGRTFKVLIQHKGMAKPELDGLKFQP
                     FFGSIL"
     assembly_gap    1289..1388
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1390..1761
                     /gene="ndhC"
                     /locus_tag="PKK81_10790"
     CDS             1390..1761
                     /gene="ndhC"
                     /locus_tag="PKK81_10790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNV33412.1"
                     /translation="MGSEAAPLNYIPILIFIVIAFAFGAVQILLGRIVRPSRPYRAKL
                     APYESGSPLFSDARVQFPIRYYIIAMLFVIFDIEIVFMFPWAVAFKKLGLVGLVEMVV
                     FIGILIVGFWYAWKKGALEWD"
     gene            1859..2338
                     /locus_tag="PKK81_10795"
     CDS             1859..2338
                     /locus_tag="PKK81_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNV33413.1"
                     /translation="MSFLERQLDANIMTTNLDALVCWARKSSLWPMTFGLACCAIEMI
                     ASVSSRYDIDRFGAGVFRASPRQSDLMIVAGTVCRKMAPVIRRIYDQMPEPRYVISMG
                     SCATSGNHYNSYAVVQGVDQIVPVDVYIAGCPPRPEALLDGLLKLQEKIQREKIFVK"
     gene            2441..4168
                     /gene="nuoD"
                     /locus_tag="PKK81_10800"
     CDS             2441..4168
                     /gene="nuoD"
                     /locus_tag="PKK81_10800"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNV33414.1"
                     /translation="MTTFPDAVRGVEVDTARNEITARVAAARIVDVARWLHDTPEASF
                     DHITDICSVDYPNDPERFEVVYQLLSLAHRRRIRLKARVTEDAPQIASVTGIWKGAEF
                     MEREVYDLMGITFVGHPDLRRILLPEDYEEGHPLRKDFPTEGRGWRSSFPFIPRLDEA
                     PEEQTEGEVPEQEKQAYLVAEHQGGTRRREELLLNMGPQHPSTHGVLRVVLELDGERI
                     VKATPDLGYLHRGVEKLAEGLHYMQVIPHTDRLDYVCAMTNNYAYVRAVEKLLDITVP
                     ERAEYVRTIVAEMQRIIGHLFWLGTQALDIGAMTVFFWTFREREVLLDMFEKLCGARL
                     TLNYYRIGGVDSDFTPDLVVRLKAFLQTFPEKVNEYNQLLMSNRIWVGRTKDVAVISA
                     EDAINFGLTGPSLRGSGVDYDVRKYEPYGVYDKVEWEVPVGKRGDTYDRYWVRMEEMR
                     QSARIIAQCLDQMPEGPIMADVPHVIPPPKAKVMRDMESLIHHFIIFTQGFKPPKGET
                     YCGTEAPKGELGFFIVSDGSPRPYRLKIRAPSFIHMGAFDHMARGYLISDIITIFGTY
                     DIVMGECDR"
     gene            4233..4772
                     /gene="nuoE"
                     /locus_tag="PKK81_10805"
     CDS             4233..4772
                     /gene="nuoE"
                     /locus_tag="PKK81_10805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNV33415.1"
                     /translation="MLKETHKAEIEDILSRYPVKRSALLPLLYMAQREQGYVTEAAMQ
                     EIAGILRLTPPQVYETATFYTMLNLKPVGTFHLQVCKSLMCALVGSDTIIGWISAKLG
                     IKPGETTPDKLFTLSIVECLAACGTGPMMQVNDDYYERLTEEKLDRILADLRQTGTCS
                     LKTGPFMWPEPANAVKREA"
     gene            4846..>5860
                     /gene="nuoF"
                     /locus_tag="PKK81_10810"
     CDS             4846..>5860
                     /gene="nuoF"
                     /locus_tag="PKK81_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNV33416.1"
                     /translation="MPKYEPILLKNMLQPGYTGSLTEYERVGGYQAIRKVLGKVSPAD
                     VTAVVMKSGLRGRGGAGFPTGVKWGFLPKDYQGPRYLCCNADESEPGTFKDRQLIERD
                     PHQLLEGMLLACYAIGAASSYIYIRGEFVLGAKILEQAINDARAAGYVGKNIFGSNTT
                     IDIWVHRGAGAYICGEETALLESLEGKRGLPRVKPPFPATHGLYNKPTVVNNVETLAN
                     LPHILNRGAEWFASIGSPPKSTGTRVFCVSGHVARPGNYEVPMGITFRELIYEQAGGM
                     RSDKKLKAFIPGGASAPFLTPDHLDVKLDFESVALAGSMLGSGGVTVMEEGTDMVWAA
                     LRLM"
BASE COUNT         1183 a         1704 c         1731 g         1142 t
ORIGIN      
        1 cttttcgcct tgacttgccg ctgaggcagc ggctaatcta accccctctt ttccctagag
       61 aatccggtag aagtgctgtt tctagcgatg gcgcatgaaa cgggaggcga ctaccgacaa
      121 gggtggtgtc actttgagca ctgggcatcc acaactcctg gcggaaatca gggctgaaat
      181 tgcagccact ggtccgattc cgttcgcccg ttttatggac ctcgccctgt atcatccgaa
      241 gtacggctac tatgttcgcc ccgtcgatga tccgacccag gaacgcatcg gatggtcggg
      301 cgatttttat acgagttccg acgtgcatcc gatcctcggc caggcgttag ccagacaggc
      361 acaacaactc gacacgctcc tcgggcatcc agaccccttc acggtggtgg agatgggagc
      421 gggcaaggga ctgctggcgc gggatttcct gaccgcttgc cggaacgcgc ccgcgaacct
      481 ccgcgaccgt cttcgataca tcttgatcga gcgcagcgcc gcaatgcgca cccagcagca
      541 aaacaatctg gcgccctggg tgggacaggc cggtcgcgtt gcctggctcg accgcctgga
      601 agatctgccg ccgaacagcg tcaccggact ctttttttcg aatgaactcg tggatgccct
      661 tcctgttcac cggctcgcag tgctcgacgg ccggccgcag gaggtctatg tcgaatatcg
      721 cgacgagcga ttcagcgagg tctaccgccc cctctccaat gaactcaccg cctacctgcg
      781 agagggcggg atcaacctgc cggacggcta tcgggctgaa atcaatctcg acgcggtcga
      841 ttggatgacg caggtggcac aggtgatggc gcgcggcgcc gtgctgacga tcgattacgg
      901 ccacaccgcc gaagacctct atggcccgga ccgcaagaac ggcacgttcc tctgctacta
      961 ccaccaaacc acctctgagg atgcgtacga ccgggtgggg gagcaggaca tgaccgccca
     1021 cgtggacttc accgccctgg cgcaaacggg ccaacaggcc ggattggacg tcaccgggtt
     1081 tacgaatcag atgagtttct tgatcggctt gggcgccgaa caattgctgg aatcgctcca
     1141 gccggaatcg ccggagtttt acgcggcgat acacctgctg cggccggacg gcatgggtcg
     1201 cacattcaaa gtattgatac agcacaaggg catggccaag cccgagttgg acggactgaa
     1261 gttccagccg ttcttcggat cgattttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnca tgggtagcga agccgcaccg ctgaactaca tcccgatcct gatttttatc
     1441 gtgatcgcgt ttgcgttcgg cgccgtccag attctcctgg gacgcatcgt tcgtccgagc
     1501 cggccctatc gcgccaaact cgcgccgtac gagagcggca gcccgctctt ctccgatgcc
     1561 cgggtgcagt ttccgatccg gtattacatc attgcgatgc tgttcgtgat cttcgatatc
     1621 gaaatcgtct tcatgtttcc ctgggccgtg gcgttcaaga agttgggact ggtggggctc
     1681 gtcgaaatgg tcgtgtttat cggcatcctg atcgtcgggt tctggtacgc ctggaaaaag
     1741 ggggcgctcg aatgggacta acggccgtaa aacgagagac gtcaaacgtg cacggtgcgg
     1801 acgatgctct gccgtctttc ctttcacgtt tcacctttca cgttccacaa ggtcagatat
     1861 gagttttttg gaacggcagc ttgatgccaa tatcatgacg acgaatctcg acgccctcgt
     1921 ctgctgggcg cgcaaatctt ctttatggcc catgacgttc ggcctggcct gctgcgcgat
     1981 cgagatgatt gcgagcgtct cctcgcgcta cgacatcgac cgattcggcg cgggtgtctt
     2041 ccgcgcatcg cctcgccaat cagacttgat gattgtggcg ggaacggtct gtcgaaagat
     2101 ggcgccggtc attcgccgca tctatgatca gatgccggaa ccgcgttacg tcatctccat
     2161 gggatcctgc gcgacctccg gcaatcacta caacagttac gccgtcgtgc agggcgtgga
     2221 ccagatcgtt ccggtcgatg tgtatattgc cggttgcccg ccgcggcctg aagcgctgtt
     2281 ggacgggttg ttgaaattgc aggaaaagat tcagcgcgaa aagatctttg tgaagtgaga
     2341 gaggaacaga ggagtatcgc ttgtgagcgc gagcttctct tgccatcggc tataggccat
     2401 cggctctttg aggaagatgc aaccgctgct tgaacgactc atgactacct ttcccgacgc
     2461 cgtccgcggc gtggaagtgg ataccgcccg aaacgagatc acggcccgcg tcgccgccgc
     2521 tcgaatcgtc gacgttgcgc ggtggctgca cgacacgccg gaggcctcgt tcgaccatat
     2581 caccgatatc tgttcggtgg actatccgaa tgacccggaa cggtttgagg tggtctatca
     2641 actcctctcg ctcgcgcacc gccgacgcat ccgattaaaa gcccgcgtga ctgaagatgc
     2701 accacagatc gcctccgtga ccggtatctg gaagggcgcc gagttcatgg agcgtgaggt
     2761 ctacgacctg atgggcatca ccttcgtcgg tcaccccgat cttcgacgca ttctcctgcc
     2821 ggaggattac gaagagggcc atccgctgcg taaggatttc ccgaccgaag gtcgtggctg
     2881 gcgcagctcg ttcccgttca ttccgcgcct ggacgaagct cccgaagaac agacggaagg
     2941 ggaagttccg gagcaggaga agcaggcgta cctcgtcgct gagcaccagg gcggcacgcg
     3001 tcgccgtgaa gaactgttgt tgaacatggg tccgcaacac cccagtaccc acggcgtgct
     3061 cagagtggtg ctggaactgg acggtgaacg gatcgtcaag gccacgccgg atctcggcta
     3121 cttgcatcgc ggcgtcgaga agctggcgga aggtctgcac tatatgcagg tcatcccgca
     3181 taccgaccgg ctcgattatg tctgcgcgat gacgaacaac tatgcctacg tccgcgcggt
     3241 ggagaagctg ctcgacatca ccgtgccgga acgggcggaa tacgtccgca ccatcgtcgc
     3301 tgagatgcaa cgcatcatcg gccacctctt ctggctcggc acccaggcgc tcgacatcgg
     3361 ggccatgacc gtcttcttct ggacgtttcg cgaacgcgaa gtgctgctgg acatgttcga
     3421 aaagctctgc ggcgcgcggc tgaccttgaa ctactatcgt atcggcgggg tggacagcga
     3481 tttcaccccg gatctcgtgg tccgcctgaa agcgttcctg cagacgttcc ccgagaaggt
     3541 caacgaatac aaccagctgc tcatgtccaa ccggatctgg gtcggacgga ccaaagacgt
     3601 ggccgtgatt tcggcggaag acgcgatcaa tttcgggctc accgggccgt cgctccgagg
     3661 atcgggtgtc gattacgatg tgcgcaaata cgagccctac ggagtctacg acaaggtcga
     3721 atgggaagtg ccggtcggca aacgcgggga cacgtacgat cgctactggg tacgcatgga
     3781 ggagatgcgg cagagcgcgc ggatcatcgc ccaatgcctc gaccagatgc cggaaggccc
     3841 gatcatggcg gacgtgccgc acgtgattcc cccgcccaag gcgaaggtca tgcgcgacat
     3901 ggagagcctg attcaccact ttattatttt cacgcagggg ttcaagccgc cgaaaggcga
     3961 aacctactgc ggtactgaag cgcccaaagg agaactcggg ttcttcatcg tcagcgacgg
     4021 aagcccgcga ccctaccgct tgaaaattcg cgccccctca ttcattcaca tgggcgcttt
     4081 cgaccacatg gcccgcggct acctgatctc ggacatcatc accatcttcg gaacctacga
     4141 catcgtgatg ggagaatgtg atagatgagg ggcgcgcgca ggaagaagac aacctcacga
     4201 ttcattgtgg tgttggcata gggacacgat agatgctgaa agagacgcac aaggcagaga
     4261 tcgaagacat tctgtcccgc tatccagtga aacggtcggc attgctgcct ctgctgtaca
     4321 tggcgcagcg ggaacagggc tacgtcaccg aagcggcgat gcaggaaatc gccgggatct
     4381 tgaggctgac tccgccgcag gtgtacgaga cggccacgtt ttatacgatg ctgaacttga
     4441 agccggtggg gacattccac ctgcaggtct gcaagtcgct catgtgcgcg ctcgtcggct
     4501 cggacacgat tatcggctgg atcagcgcga aactcggcat caagccgggc gagacgacgc
     4561 cggacaaact ctttaccttg agtatcgtgg aatgtctggc ggcatgcgga accggcccga
     4621 tgatgcaggt caacgacgac tattacgagc ggctgacgga agagaagctg gatcgcattc
     4681 tggccgacct acgacagacc ggcacctgtt cgctgaagac cggccccttc atgtggccgg
     4741 aaccggcgaa tgccgtgaaa cgtgaggcgt aaaacgtgag acgacgcaac actataacgg
     4801 gcctcaacct ttcacctttc acttttttcg tttcacgctg aagctatgcc caagtacgaa
     4861 cctattctgc tgaaaaacat gctgcaaccc ggctatacgg gttcgttgac ggagtatgaa
     4921 cgcgtcggcg gctaccaggc gatccggaag gtgctgggca aggtgagccc tgcggacgtg
     4981 actgcggtgg tcatgaagtc cggcttgcgc gggcgcggcg gcgccggctt cccgaccgga
     5041 gtcaaatggg ggttcctgcc caaggactac cagggtccgc gctacctctg ctgcaacgcc
     5101 gatgaaagcg aaccgggtac cttcaaagat cggcagctca tcgaacgtga cccgcaccaa
     5161 ttgctcgaag gcatgttgct cgcctgctac gccatcggcg cggccagctc ctacatctat
     5221 attcgcgggg aatttgtcct gggggccaag attctcgaac aggccatcaa tgacgcgcgc
     5281 gccgcgggat atgtcggcaa gaacattttc ggctcgaaca ccacgatcga catttgggta
     5341 caccgcggag cgggcgccta tatttgcggc gaagagacgg cgctgttgga atcgctcgaa
     5401 ggcaagcgcg gcctcccgcg tgtcaagccg ccgttccccg ccacgcacgg gctctacaac
     5461 aagccgaccg tggtgaacaa tgtcgaaacc ctggccaatc tgccgcacat cctgaaccgg
     5521 ggcgccgaat ggttcgcctc gatcgggtca ccgccgaaga gcaccggcac acgcgtcttc
     5581 tgcgtcagcg ggcatgtcgc gaggcccggg aattacgaag tcccgatggg tatcaccttt
     5641 cgcgaactga tctacgaaca ggccggcggt atgcggtcgg ataagaaatt gaaggccttc
     5701 attcccggag gcgcctcagc gcccttcctg acgcccgatc acctggacgt caaactggat
     5761 ttcgaatccg tcgcgttggc cgggtccatg ctggggtccg gcggtgtgac ggtcatggaa
     5821 gagggcaccg acatggtgtg ggcggccctg cggctgatgg
//
LOCUS       DAOQRM010000151         5787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_14073_length_5787_cov_14.749477, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000151 DAOQRM010000000
VERSION     DAOQRM010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14073_length_5787_cov_14.749477"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PKK81_10815"
     CDS             <1..1137
                     /locus_tag="PKK81_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV33417.1"
                     /translation="AIKALVESLLPDQLQRHARKSYVYSHDTYTEYPFQVNTHGLPPE
                     VVRECLLGFIATLSNPSSKSPAESRSFKQWIVESLGEGIAKHFMVPFNEKLWQVPLDE
                     LTSDWVSWLVPKPDVKDVVNGALGIKDKAFGYNPSFQYPATGGIKVLPEAFLPSVENL
                     SYDSELVEIETGRRRAVFRSAQGEWTEAYDRIVSTVPLPDLVRRCVDLPASLRELAAS
                     LRWVSVYNVNLAVARERISDKHWIYFPEHRYSFYRAGFPMNFSQSMGQPGCSSLYVEI
                     SHQPTERESDAALIERVRRGLEQAGVLQRSDEVVMSDVKDLYYAYVLFDRYRGRAVQE
                     LLAELERRGISSIGRYGLWEHTSMEDAIAQGQQAAARLRMKAAA"
     gene            1151..2341
                     /locus_tag="PKK81_10820"
     CDS             1151..2341
                     /locus_tag="PKK81_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV33418.1"
                     /translation="MHVVCHVITKLELGGAQEVAMQVVSGLDRRRFRPVLLAGPGGLL
                     TDEAHALEGVEVRTIPSLLREIRPLHDVRALWELVGTFRRLRPKIVHTHSSKAGILGR
                     LAAWLAGVPCILHTIHGYGVTPAQPFWQQKALIALERMVGRITTHWVAVSQADRRQGI
                     EWGLFTASKVSVVRPGIDPTAFAARIESPERDRLRAMLGVAPDQLLVGTVSCLKPQKS
                     PEDFVRVAALVCQRVPAARCVLVGDGALRPKVEALLLEQGLQDRVTLLGWRRDVAALL
                     KAFDVFVLTSQWEGLPCAILEARASRIPIVATRVGGAAEAIVEGIQGTLCPAGDVRAM
                     AERVCQILEDERFRVDLRTGTQELPEEFTIQETLKQYQSLYAYLLHATRPSEDVMKLQ
                     PNRL"
     gene            2363..3481
                     /locus_tag="PKK81_10825"
     CDS             2363..3481
                     /locus_tag="PKK81_10825"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV33419.1"
                     /translation="MNIPLLDLKAQYQSMRSEILAAIEATCDEQGFILGPRVVALEQA
                     VATYTGSAHGVGVASGSDALLLALMALGVKAGDEVVTVPFTFFATAGAISRLGAKPVF
                     IDIRPDDFNMDPALLERAITKRTKAIIPVHLFGQCAEMDAINEIARRHKIGVIEDACQ
                     AIGARQNGRHAGVLGDVACFSFFPSKNLGGFGDAGMVTTNDKALAESIAMLRVHGSRV
                     RYVHEAIGINSRLDALQAAVLLVKLKRLDEWAEGRRRNAARYVQLFTEAKLTDRIALP
                     VVGANNFHVFNQFTIRVHKRDELRSYLKDQGVGSEVYYPLPLHLQNCYRDLGYQKGAF
                     PQSERAAEEVLSLPIYAELSDEQLQYVVQAIASFYRKR"
     gene            4016..>5787
                     /locus_tag="PKK81_10830"
     CDS             4016..>5787
                     /locus_tag="PKK81_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV33420.1"
                     /translation="MTIPASFFLGMSRSSYSRLGVALALIAVIGAIDWWLPLGLTITT
                     LYVVPVLIASRIPHPRLTFWVAALASLVTILDMFDRPLFALTWVSAMNRAFALMVIWV
                     TALLCLRRQRDEAELLRINEDIEQQVQERTADLAAANQELEVLRAEAVSELVAIVKSS
                     DDAIVGMTLNGMIQSWNRGAERVYGYRAEEVLGRPISVLCPSNRLDEVPTMLDRIARG
                     EHVRNVEMVQRRKQGERIDVSLTISPVKDADGCVMGASAIARDVTVKRRIEAALRESE
                     ARFRMMADTAPVMVWMAGPDTHITFINKRWLEFTGRTVQEEIGDNWFTGIHADDLDRC
                     RKSYLQAFKSEQPFFLEYRLRRHDGEYRWIMDTGVPLFDEEGQFGGYIGTCMDLTERK
                     DMEDQLRRMLKEKESLLREVHHRVKNNLQVISSLLNLQSASIKDPVVNQLFRECQVRI
                     TSIALLHETLHRSHDLSRIKMGDYIRTLTGHLFRSYGVDPTLISLELNVDDVEFDIDT
                     GLTCGLIIDELVSNCLKHAFIDDSGGTVHIDLLDHVDGTFTLCVSDNGIGIPKDGVLN
                     NPDSLGLELVTLLAEKLDGSTELR"
BASE COUNT         1082 a         1569 c         1891 g         1245 t
ORIGIN      
        1 gcaatcaagg cgctggtcga gagcctgctc ccggaccaac ttcagcggca tgcccgtaaa
       61 tcctacgtct attcccatga cacctatacc gagtatccgt ttcaggtgaa tacgcatggc
      121 ctcccgccgg aggtggtgcg ggaatgtctc ttggggttca tcgccacgct gtcgaatccg
      181 tcgtccaagt ctccggcgga gagccggtcg ttcaaacaat ggatcgtcga gagcctgggc
      241 gagggtattg cgaaacattt catggtcccc tttaacgaga agctctggca ggtgccgctg
      301 gatgagctca cgtccgactg ggtgtcgtgg ctggtgccca agccggatgt gaaggatgtc
      361 gtcaatggcg ccttgggaat caaggacaag gccttcggct acaatccgtc gtttcagtat
      421 cccgccaccg gcggcatcaa agttctgccg gaagcctttc tcccgtcggt cgagaactta
      481 tcgtacgatt cggaattggt agaaatcgag acgggcaggc ggcgagcggt atttcgcagc
      541 gcacagggcg aatggacgga agcctatgac cggatcgtgt cgacggtccc cctgcctgat
      601 ctggtgcggc gttgtgtgga tctcccggca tcgctgcggg aattggctgc gtcgctgcga
      661 tgggtctcgg tgtataacgt gaatctggcc gtcgcccgtg agcgcatttc cgataagcat
      721 tggatctatt ttcctgagca ccgctattcg ttctatcgcg ccgggtttcc gatgaatttt
      781 tctcagtcca tggggcaacc cgggtgcagt tccctctacg tggaaatctc gcaccagccg
      841 accgagcggg aatccgacgc cgcgctcatc gagcgagtcc ggcgggggtt ggagcaggcg
      901 ggggtgctcc agcggagcga cgaggtggtg atgtcggatg tgaaggatct ctattacgcg
      961 tatgtcttgt tcgaccggta tcggggccgt gcggtgcaag agctgctggc cgagttggag
     1021 cgacgcggga tctcgtcgat cgggcggtac ggattgtggg aacatacgtc gatggaggac
     1081 gcgattgccc aggggcaaca ggcggctgcg cggttacgga tgaaggccgc ggcctgagcg
     1141 gatgaatggc atgcatgtag tctgtcacgt cattacgaaa ctggagttgg gcggcgcgca
     1201 ggaagtggcg atgcaggtcg tgtcgggcct ggaccggaga cgatttcggc cggtgcttct
     1261 tgcagggccg ggtgggctgc tgaccgacga ggcgcatgcg ctggaaggtg tcgaggttcg
     1321 cacgatcccg tcgctccttc gtgagattcg tcccctccat gacgtgcgcg ccctatggga
     1381 actggtcggg acgtttcgac gcctcaggcc aaagattgtg cacacccaca gttcaaaagc
     1441 tggtatccta gggcggttag ccgcctggct cgccggtgtc ccctgcatcc tgcacacgat
     1501 acacgggtac ggcgtgacgc ccgcgcagcc gttctggcaa caaaaagcat tgatcgcact
     1561 ggaacggatg gtcggtcgga ttaccaccca ttgggttgcg gtgtcgcagg ccgaccgccg
     1621 gcaaggcatc gagtggggtc tctttaccgc gtccaaggtc tcggtggtga ggccgggaat
     1681 cgatccgaca gcctttgcgg ctcgtattga atcgccggaa cgtgaccgtc ttcgggccat
     1741 gctgggagtg gcccctgatc agctactggt gggaacggtc tcgtgcttga aaccgcaaaa
     1801 atctcccgag gactttgtcc gcgttgctgc gctggtgtgt cagcgtgttc ccgcagctcg
     1861 ttgtgtgctc gtcggcgacg gagccttgcg gccgaaagtc gaagcgttgt tgctggaaca
     1921 ggggcttcag gatcgtgtga ccctgcttgg ctggcgccgc gatgtggctg cgttgctgaa
     1981 agccttcgat gtgttcgtcc tgacgtctca atgggaaggg ttgccctgtg ccatcctgga
     2041 ggccagagcc agccggattc cgatcgtggc cacgcgagta ggcggcgccg ctgaggcgat
     2101 cgtcgagggc attcaaggga cgctctgtcc ggccggcgac gtgcgggcta tggctgaacg
     2161 ggtctgtcag attttggaag acgagcgctt tcgcgtcgac ctccggacgg gtacgcagga
     2221 actcccggag gagtttacga ttcaagagac gctcaaacag taccagtcgc tctatgccta
     2281 tcttctgcat gccacgcgtc cgagtgaaga tgtcatgaag ttgcagccca atcgactgta
     2341 ggaaggggaa ccgggggata cgatgaacat tccgctgctt gatctcaaag cccaatatca
     2401 atcgatgcgc agtgaaatcc tggcggcgat tgaggccacc tgtgacgagc agggctttat
     2461 tctcggcccg cgggtggtgg cgctggaaca agcggtggcg acctataccg gcagtgctca
     2521 tggcgtcgga gtcgcctccg gcagcgatgc gctgttgctg gcgctgatgg ccttgggggt
     2581 gaaagccggc gatgaagtgg tcacggtgcc ctttaccttc tttgcgacgg cgggggcgat
     2641 ttccaggctt ggggccaagc cggtgttcat cgatattcgc cctgatgatt tcaatatgga
     2701 tccggcgctg ttggagcggg ccattacgaa acggaccaag gcgatcattc ccgtgcacct
     2761 cttcggtcaa tgcgccgaga tggatgcgat caatgagatc gcccgacgac acaagatcgg
     2821 cgtgatcgag gatgcctgcc aggccatcgg tgcccgtcag aatggacggc atgccggtgt
     2881 gttgggcgat gtggcctgct tcagtttttt cccgtcaaag aacctcggag ggttcgggga
     2941 cgccggcatg gtgaccacca acgacaaggc gctggcggag tccatcgcga tgttgcgggt
     3001 tcatgggagc cgggtgcgct atgtgcacga agcgatcggc atcaacagcc gattggatgc
     3061 gttgcaagcg gcggtcttgt tggtgaagct gaaacgtctg gatgagtggg cggaaggccg
     3121 tcgccggaat gctgcgcgct atgtgcagtt gttcacggag gcgaaactga cagaccggat
     3181 cgccttgccc gtcgtggggg ccaacaactt tcacgtcttt aatcaattca cgattcgagt
     3241 tcacaagcgt gacgagttgc ggagttatct gaaagaccag ggcgtgggtt cagaggtcta
     3301 ttatcccctg cccctgcatt tgcaaaattg ttatcgtgat ctggggtatc agaagggcgc
     3361 gttcccgcaa tcggaacgtg cggccgaaga agtgctctcg cttcccatct atgctgaact
     3421 ttccgacgaa cagcttcaat acgtcgtgca ggcgatcgcc tcgttctacc gcaaacgcta
     3481 acattcgcgt tgccgtccgg cgccggtttc cttccgggcc ggacggttct ttttcacatc
     3541 ctgatagccg acagtgtcac ggtcctccgc cggagaagca gtgcctcctg gcgtgtcgcc
     3601 ctgtgactgt cgttgcctgg tgcacgttgt gacgccgccg ccgacggcgc ctcctccggc
     3661 ttgactgacc gcatgtgtgc ctcccttccg gtcatcgaat cgacgacaag cgctcccgta
     3721 gcctggcgtt caagtctgaa caaccatgag gcggtgacgt acaccagggc ccgctgtggg
     3781 gcatgggctt gcgctgaatg gaattcctac gacgccgacg ttgggctcaa tatgagaacc
     3841 ggtcgcagcc tggacaatcc tggcaagcgg caccggtcaa ggcttggccg cctatcatca
     3901 gatcttggtt gccaagtgag gtctgtttcc ccgtgcgatt ggcgggtgga tgtggcgccg
     3961 gaggcatagg gattgctcat atggatgatg agggagacgc cgcgtcgttg tacgtatgac
     4021 aatacctgca tcattctttc tcggcatgtc gcggagcagc tactccaggt tgggagtggc
     4081 cctggcgttg attgcggtga tcggcgcgat tgactggtgg ttgcccctgg gattgaccat
     4141 caccacgctc tacgtagtgc cggttctcat cgcctcccgg atccctcacc cccgtcttac
     4201 gttctgggtg gccgccctcg cgtctctggt gacgattctc gatatgttcg atcggccgct
     4261 ctttgcattg acctgggtca gcgccatgaa tcgggccttt gcgctcatgg tgatttgggt
     4321 gaccgcgctg ttgtgtttgc ggcggcagcg cgacgaggcc gaattgttgc gtatcaatga
     4381 agatatcgag caacaggtgc aggagcgcac ggcggatctg gctgccgcca atcaggaact
     4441 tgaagtgctg cgagcggaag cggtgtctga actggtcgcg atcgtcaaat catcggacga
     4501 cgccattgta ggcatgacgc tgaacggcat gattcaaagt tggaatcgcg gagcggaacg
     4561 ggtctacggg tatcgcgccg aggaggtgct cggtcggccc atctctgtgc tctgtccctc
     4621 gaatcgattg gatgaggtgc cgacgatgct cgaccggatc gcccggggag agcacgtgcg
     4681 caatgtggag atggtgcagc gccggaaaca gggggagcgc atcgatgtgt cgttgacgat
     4741 ctcgccggtg aaagatgcgg atgggtgtgt catgggcgcg tcggctatcg cgcgcgatgt
     4801 cacggtgaag cggcgcatcg aagcggcttt gcgggaaagc gaggcccggt ttcgcatgat
     4861 ggcggacaca gcgccggtga tggtgtggat ggccggaccg gacacgcaca tcaccttcat
     4921 caataaacgc tggctggaat ttaccgggcg cactgtgcag gaagagatcg gcgacaactg
     4981 gtttacaggc attcacgccg acgacctgga tcgctgccgg aagtcgtatc tgcaggcgtt
     5041 caagtcggag cagccgttct ttctggaata ccggctccgg cgtcacgacg gcgagtaccg
     5101 gtggatcatg gatacgggag tgcccttgtt cgacgaggag ggacagttcg gcgggtacat
     5161 cggtacctgc atggacctga ccgagcgcaa ggacatggaa gaccagctac gccggatgtt
     5221 gaaggaaaaa gaaagtctgc tgcgggaagt gcaccatcgg gtcaagaaca acctgcaggt
     5281 catttccagc ctgttgaatt tacaatcggc ctccatcaag gacccggtcg tcaaccagct
     5341 gttccgagaa tgtcaggtgc ggatcacttc gatcgcgctg cttcacgaaa cgttgcaccg
     5401 gtcgcacgat ctttcgcgaa tcaaaatggg cgactatatc cgcaccctga ccggccatct
     5461 gtttcgttca tacggggtcg atccgaccct catttcgctg gagctgaacg tggacgatgt
     5521 cgagttcgat atcgacacgg ggctgacctg cgggttgatc atcgatgagc tggtctccaa
     5581 ttgtttgaag cacgcgttta tcgacgacag cggtggaacg gtgcacatcg atctgctcga
     5641 tcatgtcgac ggcacgttta ccctgtgcgt gagcgacaac ggcatcggga ttcccaagga
     5701 cggcgtgctg aacaatcccg attcactggg gctggaactt gtgacgctgc tggcagagaa
     5761 gctggacggc agcacggaac tccggag
//
LOCUS       DAOQRM010000152         5751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_14229_length_5751_cov_15.054600, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000152 DAOQRM010000000
VERSION     DAOQRM010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14229_length_5751_cov_15.054600"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKK81_10835"
     CDS             complement(<1..242)
                     /locus_tag="PKK81_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNV33421.1"
                     /translation="MGSKIYVGGLPYSATEQQLSDLFAVHGAVESARIITDKFTGQSR
                     GFGFVEMASSDEAQKAISALNGTDMGGRTLTVNEARP"
     gene            946..1566
                     /locus_tag="PKK81_10840"
     CDS             946..1566
                     /locus_tag="PKK81_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNV33422.1"
                     /translation="MSVRSILIHVTEVPMLGTLVRLYRGIQELRNQVIEDWTVNSRDW
                     TMPGFRAIAVHRLGTWLANRRSGVVKSGLLAVYRVLYRYVRNHYGIEIPLTVTIGRRL
                     WLVHQHGIVFHWKSEIGDDCLIRHGATIGAGYGGQKTLHKGPKLGQRVEVGPGAVIFA
                     GVKIGDDAVIGPNAVVMSDVPAGARVFAEAPRVIQLPTAHQAARKN"
     gene            1691..3688
                     /locus_tag="PKK81_10845"
     CDS             1691..3688
                     /locus_tag="PKK81_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HNV33423.1"
                     /translation="MNIARAQCAASSLTPETRGDAFRKKGQVEQAIEAYLEGVAAPPS
                     AALCLKLARCYEQMANYAEACRWALSIVDAGDDFTSWQAGWALFQRNAQKARRPAVRS
                     VKVALLGSYTTTQLCPMLCLAAGKLGIHIDLYESRYGQYQQDILDHKSGLYAFNPNIV
                     VLAVHEGDLHLPEYSQRPEEDVRKEVSRWTGLWQMVTEHSRARIVQHNFALPCEIPTG
                     HLTTRLSGSRYMMTQAVNTRLGEAAANAVSLVDCERLSALIGKQRWCDPRYWNMSKQA
                     VALEALPLLGRHTAAVIAADLGLSRKCLVLDLDNTLWGGVIAEDGLAGIKLGQGPDGE
                     AFVAFQEYLLKLKRKGVILTVCSKNNHADAIQPFEKHPEMRLKLQDIALFLANWESKP
                     DNIRTIAKTLQIGLDALVFVDDNPVEREIVRKFLPEVDVIPLPEDPSYYLRTLSDYLL
                     LETSSLTAEDTERTDQYRARAQIMELEAAAGSIEDFYRSLRMQAIVVPFDASQLPRIA
                     QLIGKTNQFNLTTRRHGMAQLEAFVLDSSYVHLALRLRDRYTDHGLVSVMIARQQGDI
                     LDIDTWLMSCRVIGRTVETTMLEQLCRRAAQLGCTSLRGTYIPTQKNAMAADAYAKHG
                     FERVGNHEGFEVWTYDLAAKGLIANTFVKPVDSWELADGAC"
     gene            3675..5246
                     /locus_tag="PKK81_10850"
     CDS             3675..5246
                     /locus_tag="PKK81_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV33424.1"
                     /translation="MAPAETPSGAPPSTPHVTTVCFTTQDLAQFSAASGDRNPLHLSE
                     EYARATPYGEPVVFGMLGVLAALGHLADRQDRLLQRLSVEFRNPLVVGMPYRLEALET
                     SVERACVKLYDATRLMLKATFTFVPGCEKRRDTHVPEAACFTEAEDRKKEDLPAGTRV
                     TGVYGPAISELVQVITRWRLSEKGATTRQLTAMLWASFVVGMQLPGKRAVFWRLELTL
                     YPEDGPQDTLLSYDVAVQDFDERFDLLHSAGTLSAAGTRCATADMWAFVRQDSPQPSL
                     RRLTDLIPRSDRLKGKVALVIGGSRGLGAAITQALASQGCTVFLNYHQCRAEAEKIRA
                     SLGDTSSLIELAQGDASEIEWCRTLRQRIVERYGGLDLLIGNASPPIRPLSFVPEKLA
                     QFQNFLTQSVSLASMPMSTFLNDLSERSGWNVLVSSAFVKDRPAEWPHYVTAKCAIEG
                     LVQWAAVHSPKVRTLIVRPPKLLTDQTNTTVGRQGAMEVERAAAAIVTRITCPDSSPT
                     VQILETFELNPEASR"
     gene            5397..5633
                     /locus_tag="PKK81_10855"
     CDS             5397..5633
                     /locus_tag="PKK81_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNV33425.1"
                     /translation="MELHDRLEEVFRQVFDNESLTLTNEMTAPDIEGWDSVAHINLMF
                     GIEQAFGVRFKGNELAEMKNIDELKQFLIGKGAS"
BASE COUNT         1286 a         1664 c         1648 g         1153 t
ORIGIN      
        1 ggacgcgctt cattgaccgt caatgtccgg ccacccatgt ccgtcccatt gagagccgaa
       61 atcgccttct gtgcctcgtc tgaagaggcc atttcgacga atccgaaacc tctcgattgg
      121 ccggtaaact tgtccgtgat gatacgggcc gattcaaccg ccccgtgcac cgcgaacaaa
      181 tcgcttaact gctgttcggt tgccgaatag ggcaacccgc caacataaat tttagaaccc
      241 atgtgggtcc tcctcttggg tagtgacatt gtcttgaacg aaggaaagaa agggaaagga
      301 cgggccgaag acgctggaca acggggcgac ttgggtctgg ctttcgatta gattcccggg
      361 caaggcaaca tcgagtcagg cctgaactat ttcaacgcat ctctcaccag gccccagcgg
      421 gagacgcggg atagtaccat gggtcccccc accatacaag ccatgaaata ggcctactaa
      481 ggtgcgggtc tgccgttgtg accaggattg actgtcacat cacaatcgac aaccaggcat
      541 aaaagaacga cgactgaatc tgacacaaag ctcacagcgg gcacagaaac cgaccgcaaa
      601 acaatcgcac accaaaaaac atcacattcc ttgcccgcac cgcggatttt atcttgtcca
      661 gtaatactgg acatatgcta agatacgtcc atgtcttcaa cacggagtat taggcctgaa
      721 gccaggctct tgccgaatgg aactatgccg agcgactccg cccgcgccca ctcgctagca
      781 cagctaccgg atcatgacgc ctagcgccct gctcaggaaa ttctccacaa ctcgcgaagc
      841 cccaccgatc atggctcatg cggttggagc cccagccact tcgccggttg ctcagatctc
      901 gttggactcc tcacctgcga agactcagcg acaagacagc accacatgag cgttcgatcc
      961 atcctcatac acgtgaccga ggtgccgatg ctggggacac tcgtcaggct ctatcgaggc
     1021 atacaggaac tacgaaacca ggtcattgaa gactggaccg tgaacagccg cgactggacg
     1081 atgcctggct ttcgagcgat cgccgtgcat cgcctcggca cctggctcgc gaaccggcgc
     1141 agcggcgttg tcaagtccgg cctgctcgcc gtgtaccgtg tgctgtatcg ctatgtgcgc
     1201 aatcattacg ggattgaaat tccattgacg gtcacaatcg gccggagact ctggcttgtc
     1261 caccaacatg ggatcgtctt tcattggaaa tcagagattg gagatgactg cctgattcgc
     1321 cacggcgcca caatcggggc gggatatggc gggcaaaaga cgttgcacaa gggtccgaaa
     1381 cttggacaac gagtggaagt gggccccggc gcggtgatct ttgccggagt caaaatcggc
     1441 gacgatgcgg tcatcggccc gaatgcggtg gtgatgtcgg atgttccggc cggcgcaaga
     1501 gtgtttgccg aggcgcctcg ggtcatacag ttgccgaccg ctcatcaggc agctcgcaag
     1561 aactaacgac ctacggccag agactgactc cgaaggaacc tcgcgctatt caccgtccag
     1621 ggatcgactc aaccacagac gcctcttatc cggtgagtgt actgcttata gaaatgacag
     1681 gccgtccctc atgaatattg cccgggcaca atgcgccgct tcatctctca cgcctgagac
     1741 acgtggcgac gccttccgca agaagggtca ggtcgaacag gcgatcgagg cctatctgga
     1801 gggcgtggcg gctcccccat cggctgcgct gtgcctcaaa cttgcgcgat gttacgagca
     1861 gatggcgaac tatgcggagg cctgtcgatg ggctctttcc atcgtcgatg caggcgatga
     1921 ctttacgtcc tggcaagcag gctgggcatt gtttcaacgc aatgcacaga aagcccggcg
     1981 accggcggtc aggtcggtca aagtcgcgct cctcgggagc tataccacaa cccaactgtg
     2041 cccgatgttg tgccttgcgg cggggaaact cggcatccac atcgacctgt atgaaagccg
     2101 atacgggcaa taccagcagg acattctcga ccacaagagc gggctctatg cgtttaaccc
     2161 gaatatcgtc gtccttgcgg ttcacgaggg tgacctccat cttcctgaat acagccagcg
     2221 ccctgaagaa gacgttcgca aagaagtgag tcgctggacc ggcctctggc agatggtgac
     2281 tgaacactcc cgcgcccgaa tcgtgcaaca taactttgca ttgccctgcg agatccccac
     2341 aggccatctg accaccaggc tgtccggctc tcgatacatg atgacgcagg ccgtcaatac
     2401 gaggctgggt gaagcggctg cgaacgccgt ctccctggta gattgcgagc gtctttcggc
     2461 gctcattgga aagcagcgat ggtgcgatcc gcgctattgg aatatgtcga aacaagcagt
     2521 cgcactggag gccctgcctc tgctcggaag acatacggcg gctgtgattg ctgcggatct
     2581 cgggctcagc cgtaaatgtc tggtgctgga tttggacaat acgttatggg gcggtgtcat
     2641 tgcggaagac ggattggcgg gcatcaagtt agggcaaggg ccggacggcg aggcgtttgt
     2701 ggcgtttcag gagtatctgc tgaagctcaa acgcaaaggc gtgatcctga cggtctgctc
     2761 gaagaataac cacgccgatg ccatacaacc cttcgaaaaa catccggaga tgcgactgaa
     2821 gctgcaggac atcgccctct tccttgccaa ttgggagtcc aagcccgaca acatccgcac
     2881 catcgccaag acgctgcaaa tcggtttgga tgcgttggtc ttcgtcgatg ataaccctgt
     2941 cgagcgcgag attgtgcgca agttcttacc cgaagtcgat gtcatcccgc tgccggaaga
     3001 cccctcctat tacctcagaa ctctgtcgga ctatctcctg ctcgagacga gctcattgac
     3061 cgccgaagac acggagcgga ccgaccaata tcgggccaga gcgcagatca tggagttgga
     3121 agcggctgcc ggttccattg aagattttta ccgcagcctt cggatgcagg ccatcgtggt
     3181 gccgttcgat gcatcacagt tgccgcgcat tgcccaactc atcggcaaaa ccaaccaatt
     3241 caatctcacc actcgacgtc acggcatggc gcaactggaa gccttcgtcc tggactcgag
     3301 ttatgtccat ttggcgctgc ggttgcgtga tcgctacacc gaccatggac tggtcagcgt
     3361 gatgatcgcg cgacaacagg gagatatcct cgatatcgac acgtggctca tgagctgccg
     3421 ggtcatcggc cggaccgtgg agacgacgat gctggaacag ctctgtcggc gcgccgcgca
     3481 gcttggatgc acctcgcttc gaggcaccta cattccaacg caaaagaatg cgatggcggc
     3541 ggacgcttat gccaagcatg gcttcgagcg agtcgggaac catgaaggat tcgaagtctg
     3601 gacctatgac ctggcggcca aaggcctgat tgccaatacg ttcgtgaaac ctgtcgactc
     3661 ctgggagctc gcggatggcg cctgctgaaa cgccgtccgg ggctcccccc tccacgccac
     3721 atgtgaccac agtgtgtttc acgacgcagg atctcgccca gttcagtgca gccagcgggg
     3781 atagaaaccc cctgcatctc tccgaagaat atgcgcgagc cacaccctac ggcgagcccg
     3841 tggtattcgg catgcttggc gtgctggcgg cactcggcca tctcgccgac cgtcaggatc
     3901 ggctcctgca acgcctctcg gtcgagtttc gaaatccgct cgtcgtcggc atgccttatc
     3961 gtctggaggc gctcgaaacc tcggtagaac gggcgtgcgt caaactctat gacgccacgc
     4021 gcctgatgct gaaggcgacg ttcacatttg ttcctgggtg tgagaagagg cgtgacacgc
     4081 atgttcctga agcggcctgt tttacggaag cagaggatcg gaagaaggag gaccttccgg
     4141 caggtacccg tgtgaccggc gtctacgggc cggcaatcag cgaactggta caggtgatca
     4201 ctcgatggag actctccgaa aaaggcgcca caacgagaca gctcacggcg atgctgtggg
     4261 ccagctttgt cgtcggaatg cagcttcccg gcaagcgcgc cgtcttctgg cgattggaac
     4321 tcaccttgta tccagaagac ggaccacagg acacgctgct ttcctatgat gtggcggttc
     4381 aagacttcga cgaacgattt gatctcttgc acagtgctgg cactctctcg gcagccggca
     4441 cccgttgtgc gacggccgac atgtgggctt ttgtacgaca ggattctccg cagccctccc
     4501 ttcgtcggct taccgatctg atcccgagat cagaccggct gaaggggaag gtcgcgttag
     4561 tcatcggtgg aagccgtgga ctcggtgcgg caatcactca ggcgctggct tcgcagggct
     4621 gcacggtgtt tctgaactac catcaatgca gggcggaggc ggaaaagatt cgagcgagcc
     4681 ttggagacac ttccagcctc attgaattgg ctcaggggga tgcgtcggaa atcgaatggt
     4741 gccgaacgtt acggcagaga attgtcgagc gatacggcgg acttgatctg ctgataggca
     4801 atgcatcgcc gcccattcgc ccgctgtcgt tcgtgcctga gaagctcgcg cagtttcaga
     4861 acttcctcac gcagagtgtc tcgctggcca gtatgcccat gtccacgttt ctcaacgact
     4921 tgtccgaacg ctccggctgg aacgtgcttg tctcttcggc gtttgtgaaa gatcgtcctg
     4981 cggaatggcc ccactatgtc actgcgaaat gtgcgatcga gggcctggtg cagtgggccg
     5041 cagttcactc tccgaaggtc cgtacgctta ttgttagacc accgaagttg ttgacggatc
     5101 aaaccaacac cacggtggga cggcagggag caatggaggt cgaacgcgcc gccgctgcga
     5161 tcgtcacacg gatcacttgc cccgactcat cacccaccgt acagatactc gagacgtttg
     5221 agctgaaccc tgaggcttcg cgatgaccct ccgggaactt gcgcgacgaa gccgatcgac
     5281 ctcgtgccgc ggctgcatga agcggattta cagaagcaaa ggtgacggga agatggcttc
     5341 ccggccacga catgatgatg gacatgtgac gacaccactg agagttgggg gaatgaatgg
     5401 agctgcatga tcgacttgaa gaagtgtttc gccaagtgtt cgacaatgag agcctcacgc
     5461 tgaccaatga gatgacggca cccgatatcg aaggctggga ctccgtggcc catatcaatc
     5521 tgatgttcgg catcgaacag gcgttcggag ttcggttcaa gggcaatgag ttggccgaaa
     5581 tgaagaatat cgacgaactc aagcaattcc tgatcggcaa gggagccagc taacccatac
     5641 cgcagccacg ggtcgatcaa cgaacgaagc aacaggaacc ttcgccgatc tgattgagat
     5701 gagcaacacc gctgtcagcg ttaccggtct ctccaagcgc tatcgcctgg g
//
LOCUS       DAOQRM010000153         5724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_14311_length_5724_cov_19.889398, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000153 DAOQRM010000000
VERSION     DAOQRM010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14311_length_5724_cov_19.889398"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="PKK81_10860"
     CDS             <1..1506
                     /locus_tag="PKK81_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNV33426.1"
                     /translation="TGMEKQAEIDAAAQAFGVDAKTAKRLIKHPFYSYPGGHSFFDLY
                     IDVIDGLHRLGRAHPGEVLCLYTHSSTLRALRIYLDPRPFREAFSEFGEYKEGQDNVV
                     LLTLENGKLSGYSTAVGLIESERVAREAWVTVERERSQRVTLKPQRIKRLLALVSGGD
                     FGGGGAALKELRVTGNRFGLEVFFVRHGFLGLANNWIDPVTEEDTRGMGSHASSPIGS
                     SRFEDFKDEEVQRAAMRSLAPYLADSALVVLGGDGSLRGARAIHETYGVQVVGIPGTI
                     DNNIAGTTSLGFHSAIALANQSIESLKATSAAMGSIFFVEVMGAGSGHLALACAYQAR
                     AEGILVNEHPDPDAYIEEIVLGTLKRTLGVPNKSHIFVVAERTPHRHHREGGVHGLVD
                     YVANTIATWPQRREKSGHYALATATKATILGHTLRGAPPTPEDKMIAQHLAYETVRRL
                     VEQPDTIVGCMVAYHDSNRLETIPLHAVAPKPFDWELFTRMHGTELAPDRV"
     gene            1783..2247
                     /locus_tag="PKK81_10865"
     CDS             1783..2247
                     /locus_tag="PKK81_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNV33427.1"
                     /translation="MMSIRRITRLLLWLCATVALSLGVSFATEPGQTLLDAPTPILTP
                     APDQTPQTQNGSVRAGPRTTTRSDQASIPLTARETLSAIETRHGDPPPGYIGGRTFQN
                     RERVLPRGRYREYDVHPKVPGKNRGAERIVIDQRTGKAYYTADHYRTFVPMN"
     gene            2254..2793
                     /locus_tag="PKK81_10870"
     CDS             2254..2793
                     /locus_tag="PKK81_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNV33428.1"
                     /translation="MSLIALQSIKKPWAHLLVHAEGEALDKILSVPAHFVTKTIAGKK
                     CKTKAGLLDEFSRVFSFPDYFGHNWDALEECLADLDWLPAKGYLVVVTDADQVLTKPD
                     EEDDFETFVEILSEAGEAWSLKESDDATGDGLPFHAVLAVSDRHKRSRHNWFAPPLAM
                     ERKPLKSGPAKGTKKKPPR"
     gene            2830..3237
                     /locus_tag="PKK81_10875"
     CDS             2830..3237
                     /locus_tag="PKK81_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNV33429.1"
                     /translation="MGLLDQLGQAAAGMMGGGDKSPLMQAVVNLLGQNSSVGGLSGLV
                     QAFQKNGLGDIVNSWVSTGKNLPVSAEQIQQGLGGDLLKQLASQAGLSSEAAGSQLAN
                     LLPGLIDKLTPDGKMPDTTLIEQGLNLLRGKLG"
     gene            complement(3286..3528)
                     /locus_tag="PKK81_10880"
     CDS             complement(3286..3528)
                     /locus_tag="PKK81_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33430.1"
                     /translation="MKVLCAWCVRDGKPAFLREKFPLEDPSETHGLCGDHFTSLSASV
                     GKIVTPRVWMLSRMHDLSWGLTRWAQRMMGRFFSLC"
     gene            complement(3770..4000)
                     /locus_tag="PKK81_10885"
     CDS             complement(3770..4000)
                     /locus_tag="PKK81_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33431.1"
                     /translation="MKVICSWCQSEGRPGLVREKAPLADLRETHGICSDHLQQMAVGR
                     DPLIPGYDEGSLPRHESVVIRRSDLAAVACYE"
     gene            4266..5549
                     /gene="hemL"
                     /locus_tag="PKK81_10890"
     CDS             4266..5549
                     /gene="hemL"
                     /locus_tag="PKK81_10890"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNV33432.1"
                     /translation="MKTQRSEQLFADAQLIIPGGVNSPVRAFRSVGGQPRFIARAKGS
                     RLYDVDGNSYIDYVLSWGPMILGHAPATVTKAIQQAAGNGTSYGAPTELEIQLATMIR
                     EALPSMEMVRLVSSGTEAVMSAIRVARAYTKRDGILKFEGCYHGHSDYLLAKAGSGLT
                     TLGIPDCPGVPEDFTKHTLTAPYNDLATTEKLIKKHYRQLACVIVEPIAGNMGVIPPS
                     PEFLYALRKLTDAHGMLLIFDEVISGFRVQYGGAQTLYDIKPDLTILGKIIGGGLPVG
                     AYGGAKDIMKMIAPSGPVYQAGTLSGNPLAVTAGIETLKRLKKPGTYEQLEQRSVALS
                     EGVGNAARKAGVPLTQTRVSSMMCAFFTQGPVTDWASAKKADTKAYAKFFHQMLDAGI
                     YLAPSQFEAAFMSVAHTPKDIDRTISAAQAAFKNL"
BASE COUNT         1227 a         1760 c         1663 g         1074 t
ORIGIN      
        1 accggcatgg aaaagcaggc ggagatcgac gctgccgcgc aggccttcgg cgtcgatgcg
       61 aagaccgcca agcggctcat caaacatccc ttctatagct atccgggcgg gcattcgttc
      121 ttcgacctct atatcgatgt gatcgatggc ctgcatcggc tgggccgtgc ccatcccgga
      181 gaagtgctct gcctctatac ccacagttcg accctgcgcg cactgcgtat ctatctggac
      241 ccgcgtccgt tccgcgaagc gttttccgaa ttcggcgaat acaaggaggg acaggacaat
      301 gtcgtcctcc tcacgctgga gaacggcaaa ctctccggct actccacggc cgtcggcctg
      361 attgaaagtg aacgggtcgc ccgcgaagcc tgggtgacgg tcgagcggga gcggagccag
      421 cgcgtcacac tcaagcctca acgcatcaag cgcctgctgg cactcgtgtc gggcggggat
      481 ttcggcggcg gcggagcggc gttgaaagaa ttgcgcgtga ccggaaaccg cttcggcctg
      541 gaggtctttt tcgtccggca cggtttcctc ggattggcga acaactggat cgatcccgtc
      601 acggaagaag acacacgcgg catgggcagc catgccagca gccccatcgg cagcagccgt
      661 ttcgaagatt tcaaagacga ggaggtccaa cgcgccgcca tgcggtctct tgcgccttat
      721 ctcgcagact cggcgctcgt ggtgctggga ggcgacggca gcctgcgggg cgcccgggcg
      781 attcacgaga cctacggggt gcaggtcgtc ggcattcccg gcaccatcga caacaacatc
      841 gccggcacga cttccctggg gtttcattcg gccatcgcgc tggccaatca atccattgaa
      901 tccctcaagg cgaccagcgc ggccatgggc agcatctttt tcgtggaggt catgggagcg
      961 gggtccggcc atctggcatt agcctgcgcc tatcaggccc gggcggaagg gatcctcgtg
     1021 aatgaacacc ccgaccccga tgcctacatt gaagagatcg tcctgggcac gttgaagcgt
     1081 accctgggtg tacccaacaa aagccacatc tttgtcgtgg cagagcgaac tccccaccgg
     1141 caccaccggg aaggcggtgt ccatgggctg gtcgactatg tggccaacac catcgcaacc
     1201 tggccgcaac gtcgggagaa gtccggccac tatgcattgg ccacggccac gaaggcgacg
     1261 atcctggggc atacgctcag gggcgccccc ccgacaccgg aagataaaat gatcgcgcag
     1321 catctcgcct acgaaaccgt ccgccggctt gtcgaacaac cggacacgat cgtcggctgc
     1381 atggtcgcgt accacgattc caaccggctc gaaacgattc ctctccatgc cgtcgcgccc
     1441 aaaccgttcg actgggagtt atttacccgc atgcacggga cggagttggc ccccgacagg
     1501 gtctagctcg gatgattcat gaatgccgtt gcgaggcgtt cgagactgaa acccgccggg
     1561 gcttctcgca gggcgcacgg cgcgatgaat aaatcgcgcc acgtctgtgc gcgccggcga
     1621 gttggtgagc cggccggttc ttgatatcgc catccatagc agcccatcga ggaggccgaa
     1681 cgagaaaagc cccgccggac gagaggatcg gacggcgtag cacgttgacc cctcccatgt
     1741 gacatgttag ggtcgtcctg ttgatgccac cggttgcgaa tcatgatgtc cattcgacgc
     1801 atcacacggc ttcttctttg gctctgcgcc acagtcgctc tctccctggg cgtctccttc
     1861 gcgacagaac cgggccagac actcctcgat gcgccgaccc cgatcctcac accggctcct
     1921 gatcagacgc cgcagacaca gaacggttcc gtgcgcgccg gaccccggac caccacgcgc
     1981 agcgaccagg catccatacc gctcactgcg cgagagacgc tgagcgctat tgaaacgcgg
     2041 catggcgatc cgccgccggg ctatatcgga ggacgcacct ttcagaatcg tgaacgcgta
     2101 ctgccgcgcg ggcgctatcg cgagtacgat gtgcacccca aggtgccggg gaagaatcga
     2161 ggcgctgaac ggatcgtcat cgaccaacgc accggcaagg cctattacac tgccgaccac
     2221 taccggacat ttgttccgat gaattgagga gacatgtcgc tcattgccct ccaatcgatc
     2281 aagaaaccct gggcgcatct cctggtgcat gccgaaggag aggcgctcga taaaattctc
     2341 tccgtccccg cgcacttcgt caccaaaacc atcgccggca agaaatgtaa aacgaaagcg
     2401 ggcctgctgg atgaattcag ccgggtcttc tcgtttccgg actacttcgg ccacaactgg
     2461 gatgccctcg aagaatgttt ggccgatctg gactggcttc cggccaaggg ctatctcgtg
     2521 gtggtgacgg atgcggatca agtactgacg aaacccgacg aagaagacga ctttgaaacc
     2581 ttcgttgaga tcttgagcga agccggtgaa gcctggagcc tgaaagaatc cgacgacgcg
     2641 acgggagacg gcctgccgtt tcacgccgta ctggccgtct ccgatcgcca taaacggagc
     2701 cgccacaatt ggttcgcgcc gccgttggcc atggaacgca aaccactgaa gtccggaccg
     2761 gccaagggca caaaaaaaaa gccccctcgg tagacaatcg actcaatcca cttacgccag
     2821 gaggttacga tgggacttct cgatcaactc ggacaggcag cagccggcat gatgggcggc
     2881 ggcgataaga gccccctgat gcaggccgta gtgaacctgc tagggcagaa cagttccgtc
     2941 ggggggctga gtggactcgt gcaggcgttt cagaagaacg gcctgggaga tatcgtgaat
     3001 tcctgggtca gcacaggaaa aaacctcccg gtctcggcgg agcagatcca gcagggcctt
     3061 ggcggcgacc tcctgaagca actggcatct caagcgggat tgagctcgga agcagccggg
     3121 agccaactgg ccaatctgct tcccgggctc atcgataagc tgaccccgga cggcaagatg
     3181 ccggacacca cattgattga acagggattg aatctcttgc gaggaaaact cgggtaggac
     3241 tcgattcccg gcactcattt cagggtgccg ggtggccctt ccgcgctagc agagagagaa
     3301 gaaccgcccc atcatccgtt gggcccaccg cgtcagtccc caggagaggt cgtgcatgcg
     3361 tgagagcatc cagactctcg gtgtgacgat tttccccaca ctcgcactga gcgaggtgaa
     3421 atggtcacca cagagcccat gcgtttcact gggatcctcc aacgggaatt tctcccgcag
     3481 aaacgccggc ttcccgtctc gtacacacca ggcacagagc actttcacca cttcactccg
     3541 ttcgttgcga tgcgaccatc cacgttgggg gcgcaagagt cgcaccagcg ggcatcggcc
     3601 aagaagtatt caatgttttc agcaggatcg ttcgcgtgaa ccaatcaggc ttgtccgttg
     3661 aggccgtcaa tagcgtggca ttccggcagt gattgacgtt ttttgtcgtt ctcgttgggg
     3721 aatccaagag cgatgacggt gggacggtcg catccaagta cacgcttgct cattcataac
     3781 aggccacggc ggccagatcg cttcgacgga tgacgacaga ttcatggcgc ggcagcgagc
     3841 cttcatcata tccggggatc agcggatccc ggccgaccgc catttgctgg aggtggtcgg
     3901 agcagattcc gtgggtctcg cgcagatcag ccaacggagc cttttctcgc accaaccccg
     3961 gtcggccttc gctctggcac caggaacaga ttactttcat cgtgttctcc tacgcagtga
     4021 tacccaagac tcagcaagac ttgcgccata tggtcggcgg tctctaactc cttgtttctc
     4081 tgtgctaaca ttgccttata ccgctacgaa tcatcgtggg tgtatctatt cggataatcg
     4141 gctgtatcga atcgtggatg agtagaggca ccggtgccga ctcacagaag actgtccaca
     4201 attgaccgtt tttattttcc cgtggtaggt tggcgcccca actcacggct cgatgaaaga
     4261 ggcccatgaa aacacaacga tcggaacaac tcttcgcaga cgcgcaactc atcattcccg
     4321 gcggggtgaa cagcccggtt cgggcctttc gctcggtggg cgggcaacct cggttcattg
     4381 cacgcgccaa aggctcacgg ctctacgacg tcgacgggaa cagctacatc gactacgtcc
     4441 tgtcctgggg accgatgatt ctcggccatg cgccggcgac cgtcaccaag gccattcaac
     4501 aggccgccgg aaacggtacc agctatggcg cgccaaccga gttggaaatt caactggcca
     4561 cgatgattcg cgaggccctg ccgtctatgg agatggtgcg gctggtgagt tctggaaccg
     4621 aagcggtcat gagtgccatt cgagtcgccc gcgcctacac gaaacgcgac ggcatcctga
     4681 aattcgaagg gtgttatcac ggccacagcg actacctgct ggccaaggca gggtcaggtc
     4741 tcaccacact tggcattccc gactgccccg gtgtgccgga agacttcacc aaacacacat
     4801 taaccgcgcc ctataacgac ctggccacca cggaaaaact gatcaagaag cactaccgtc
     4861 agcttgcctg cgtcattgtc gaaccgatcg ccggaaacat gggtgtgatt ccgccctctc
     4921 cagagttctt gtacgccttg cgcaagctga ccgatgccca cggcatgtta ctgatttttg
     4981 acgaagtcat ctcaggattc cgcgtgcaat acggtggtgc gcagacgctg tacgacatca
     5041 agccggatct gacgatcctg gggaaaatta tcggcggggg acttccggtg ggtgcctacg
     5101 ggggcgcgaa ggacatcatg aagatgattg ccccgtcggg accggtctat caggccggca
     5161 cgttgtcagg gaatccgctg gccgtcacgg ccggcatcga aacgctgaag cgcctcaaga
     5221 aaccaggcac ctacgaacag ctcgaacagc gttcggtggc cctctcggag ggagtgggga
     5281 atgccgcgcg aaaggccggg gttccgctca cgcaaacccg cgtcagttcg atgatgtgtg
     5341 cgttcttcac tcaggggccg gtcacggatt gggcatcagc caagaaagcc gacacgaagg
     5401 cctacgcgaa gttctttcac cagatgctgg acgccggaat ttacctggcc ccgtcacagt
     5461 tcgaggccgc gttcatgtcg gtggcccata cgcccaaaga catcgaccgc acgatcagcg
     5521 ccgctcaagc tgcgttcaag aacctctgac ccggcatgga gcgccgggcg ttgcccggcg
     5581 ctcgtgaagc gtcgttcgcg aatcctgcat ttcgtatcga aaccgatacg aaatgcatcc
     5641 attcagatac agatcgcgat tcattcccat cgaattcacc gcacgacttc aggatgctca
     5701 aaacggtttc ccagcaaggc cgca
//
LOCUS       DAOQRM010000154         5519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_15112_length_5519_cov_13.531662, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000154 DAOQRM010000000
VERSION     DAOQRM010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15112_length_5519_cov_13.531662"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PKK81_10895"
     CDS             <1..704
                     /locus_tag="PKK81_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV33433.1"
                     /translation="FGHVHTTFRPPTFANAVDPTSGTTQDLSAERALSSDIGIRSNLH
                     PGVSAEVALFRTEFSNQIVQQGSQFVNAGKTLMEGIESTVSLDWGEIVRPLQGFVTRG
                     SITLLRPKSLFGSTDGKDLPYAPRMTFYGLVGYYHPTGASIEADAMYVAQQFTDGANT
                     QTENALGTTGAIPSYTIWNLRLNYAPPKAKWAIFAGVRNLSDESFIAQRTTGSFIGIQ
                     PGEIRNFYGGVSWRF"
     gene            797..1564
                     /locus_tag="PKK81_10900"
     CDS             797..1564
                     /locus_tag="PKK81_10900"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HNV33434.1"
                     /translation="MLSILAGPSLAWAHFVWVEADATAPAETGQPLKIYFGEYSEFLR
                     EERGGRLDSIDGVTLRVQDSKQGTTEVALTKQVNHFAGALPSCLPGRNAAVAQQAQAP
                     VQDLRKHDIGIVKPMFYARTYFMCLEEGRINEHERDQAAPMALDMIPLTKGLNLATGR
                     VSHVPGGEIVVKGFFKGQFLPNATILVHAPIGWDKELKTDGEGIVAFTPLWPGRYVLE
                     LIHVEKTPGEFQGKTYEALRHRSTLAIQVRAERSGEK"
     gene            1565..2359
                     /locus_tag="PKK81_10905"
     CDS             1565..2359
                     /locus_tag="PKK81_10905"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNV33435.1"
                     /translation="MSRGGAERIVRRAHRWLGIGALGFLLLSVVTGLLWADAKFLYWD
                     EHYKEKVRPLTGPPVDSATLPLDRALVLAKEAVGGRALVEQATLRTDFGRLFYEVKLR
                     VEGVSTTLLLDAMNGERLSPITSELAPTIAAQYVRSPATVVGVEIERYTPRKKKRAYD
                     AARVRFDDDGATEIVLDRQTGEILEDEGRWRKVHFAVMQLHQLNFFGFEKTLLNIPGV
                     PILLMSLSGLLLWGFHRARARRAAVDGLAAAVSSRVPTAPEETTHA"
     gene            2435..4468
                     /locus_tag="PKK81_10910"
     CDS             2435..4468
                     /locus_tag="PKK81_10910"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV33436.1"
                     /translation="MTIRTIKGRIVLAIVLVGCIPLVIGLVLASMSGMRSLRDVIGGN
                     FQAIAEQAADRLTMLVQSEVQGVRLLASAPLRVRQPVEAANLSYKGEWADSQRLIQER
                     AKEWEKGHDSAAGLLNSELSRFLLETKVRDGDKMVGLLITDRYGALVAASSEPDHYSL
                     SQESWWEALQAGGLDRVYVSGLIPGQEGSFRSPEETIDIAVPILDDHQHAVIGAIKAS
                     YRFDSLFAMIKEIRIGQTGHAMLFDAAGQPLVCPILPRRAHRIPSQLMAMIVSSNPGW
                     GIAEDDGHGATDTVVGYAPVTGLSLPDNSWHVFVRQQPEETYAPIRDQLRNLALIGLV
                     MLCLLWAMGRYVAARIARPIQVLKSGVEAISQGTYDGPLDIKTGDEFEELAVAVHRMA
                     DRLQSSRTELESLNAELVNRVEEKTAEITRQMRKLELSERLATLGKVASGIAHEINNP
                     LGIILNRVECMEADAAQAGVPEEVGRDLVTIRTQAERISRVTRSILTFSRGTVSMLKP
                     LDLNCVARTCVAMAGERVAALAVRIDAQLAPEVSPVMGDRDRLETVLLNLINNAIDAV
                     TGSDSQGIITVSTARMPIDGDEWVRISVSDNGPGVPTEILDRIFDPFFTTKPAGQGTG
                     LGLFLSYGIVSDHRGRIEVRNHAVGAVFDVYLPAVAFGNRVHEGTSWGLQEKS"
     gene            4444..>5519
                     /locus_tag="PKK81_10915"
     CDS             4444..>5519
                     /locus_tag="PKK81_10915"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV33437.1"
                     /translation="MGIAGKILIVDDEQDALENCRRILSRVPYDCLTECDPARALDVI
                     QRERPGLILTDLRMPDLDGIEVLAAAKRFDPSVQVVLLTAHATVQTAVTSMRYGAFDY
                     ITKPFTSADLVSVARRAFGEDGAGPVAGAVGTRELLEPPEQARPGKRPGLSRVIGESP
                     AMHSVFNLIEKVAPTHSNVLVYGESGTGKELVARAIHDASLRADRPFIPVDCVSLPDT
                     LLESELFGHEKGAFTGAHVSKPGLFEVAAGGTVFLDEVSGMSQTLQSRLLRVLQERQM
                     RRVGGIRFLEVDVRVIAASNQDLESACRRGEFREDLYYRLNVIPIVLPPLRTREGDIL
                     LLAREFLRRFMKQQRLGLAFVPEL"
BASE COUNT         1025 a         1541 c         1828 g         1125 t
ORIGIN      
        1 tattcgggca tgtgcacacc acgttccggc cgcccacctt cgcgaatgcg gtcgatccga
       61 cgagcggaac cacgcaggac ctctcggcgg aacgcgcgtt gagttcggat atcggtatcc
      121 gctcgaatct ccaccccggc gtaagtgcgg aagtcgccct gtttcgaacc gaattcagca
      181 accaaatcgt gcagcagggc agtcagttcg tcaacgccgg aaagacactg atggagggca
      241 tcgagagcac cgtcagtctc gattggggcg agatcgtccg cccattacaa ggcttcgtca
      301 ccaggggcag catcacgctc ttgcggccga aatcgttgtt cggatcgacg gacggaaaag
      361 accttcccta tgccccgcgc atgacgttct acggtttggt ggggtattac cacccgaccg
      421 gtgcgtcgat cgaagccgat gccatgtatg tcgctcagca gtttaccgac ggcgcgaata
      481 cccagacgga aaatgccctg ggaacgaccg gcgctatccc ttcctacacg atttggaacc
      541 tccggctcaa ttatgctccg ccgaaggcga agtgggccat tttcgccggc gtacgcaacc
      601 tcagcgacga atcgttcatc gcgcaacgta ccaccggcag cttcatcggc attcagcccg
      661 gagaaatccg gaacttttac ggcggcgtgt catggcgatt ttaagcagca tgcgcagtcc
      721 cttccgggga ttctctatca aaggagtgtt gatgatggac aggagaagat gtgcagtcgg
      781 tctgtggagc gtgattgtgc tgtctatcct tgcggggcct tctttggcct gggcccattt
      841 cgtgtgggtc gaggccgacg ccaccgcgcc ggcggaaacg gggcagccgc tcaagatcta
      901 tttcggcgaa tacagcgaat ttttgcgtga agagcgcggt gggcgtctgg attcgatcga
      961 cggagtcacg ctacgtgtgc aggactccaa gcagggtacg actgaagtgg ctctgaccaa
     1021 acaggtcaat cactttgccg gggcgctgcc gtcttgtctg cccggccgga acgcggcggt
     1081 ggcgcagcag gcgcaggccc cggttcagga cttgcgcaag cacgacatcg gcatcgtcaa
     1141 accgatgttc tatgcgcgga cctatttcat gtgcctggaa gagggccgca tcaacgagca
     1201 tgagcgggat caggccgcgc cgatggccct cgacatgatt cccctgacca agggactgaa
     1261 tctggcgacc gggcgagtca gccacgtgcc gggcggtgaa atcgtcgtca agggtttctt
     1321 caaggggcag ttccttccga atgcgacgat cttggtccat gcgccaattg ggtgggacaa
     1381 ggagttaaaa acggatgggg aagggattgt ggccttcaca ccgctctggc cgggacggta
     1441 tgtcctggaa ttgattcacg tcgaaaaaac gccgggtgaa tttcagggga agacatacga
     1501 ggcgcttcgg catcggagca ccctggcgat tcaggtgcgg gctgagcgct caggggagaa
     1561 gtaaatgagc cgcgggggag ctgagcggat tgtcaggcgg gcgcatcgat ggctcggcat
     1621 aggggcgctt ggttttctcc tgctgtcagt cgtgacgggg ctgctctggg cggatgcgaa
     1681 gtttctctat tgggatgagc actacaagga aaaggtgcga ccgctcacgg ggccgccggt
     1741 cgattccgcg acactgccgc tcgaccgggc gctggtcctg gcgaaagagg cggtgggagg
     1801 tcgcgcgctg gtcgagcagg ccaccttgcg cactgacttt ggccggctct tttatgaggt
     1861 gaagctacgg gtggaagggg tctcgacaac tttgctgttg gatgccatga acggtgaacg
     1921 gctgtcgccg atcacgtcgg agcttgcccc gacgattgcc gcgcagtatg tgcggagccc
     1981 ggcgacggtg gtgggcgtgg agatcgaacg gtacacgccc agaaagaaaa agcgcgccta
     2041 cgatgccgcg cgtgtccggt tcgatgacga tggcgccacg gagattgtgc tggaccgaca
     2101 gaccggcgag attctggaag atgagggccg ctggcgaaaa gttcactttg ccgtgatgca
     2161 attgcaccag ctgaattttt tcggcttcga aaaaactctg ctcaatattc caggcgtgcc
     2221 gattctgctg atgagtctat ccggcctgct cttgtgggga tttcatcgtg ccagggcgcg
     2281 gcgagcggct gtcgacggcc tggcggccgc ggtgtcgtcc cgggtgccga cggcaccgga
     2341 ggaaaccacc cacgcctgac cccatcggtg ccgtgacggg gtgcggtggc gttttctttt
     2401 gccggttgtt tagaatgtca cgctggaggt cggcgtgacg attcgtacca tcaaaggccg
     2461 cattgtgctc gccatcgtgc tggtgggctg tatcccgctg gtgatcggcc tggtgctggc
     2521 gtcgatgtcc ggcatgcgct ccctccgcga tgtgatcggt ggcaattttc aggcgattgc
     2581 cgaacaggcc gccgatcgtc tgaccatgct ggtccagagc gaagtgcagg gggtgcggtt
     2641 gctcgcctcc gcgcccttac gggttcgcca gccggtcgag gctgccaacc tgtcgtacaa
     2701 aggcgagtgg gccgattcgc agcggctgat tcaggaacgc gcgaaggagt gggagaaagg
     2761 gcatgacagc gccgccggtc tgctgaattc agaattgtct cgcttcctgc tggagaccaa
     2821 agtccgcgac ggtgacaaga tggtggggct gctgatcacc gatcgctatg gggcgctggt
     2881 cgcagccagc tcggaaccgg accattattc cctcagccag gaatcctggt gggaggcctt
     2941 gcaggccggt gggctggacc gggtctacgt cagcgggttg attcccggac aggaaggatc
     3001 cttccgctcg cctgaagaaa ccatcgatat cgcggtcccg atcctggacg accaccagca
     3061 tgcagtcatc ggtgccatca aggcgtccta ccggttcgat tccctcttcg cgatgattaa
     3121 agagatccgc atcgggcaga ccggccatgc catgctgttc gatgccgcgg gtcagccgct
     3181 cgtctgtcct atccttcccc ggcgcgcgca tcggattccc agtcaactga tggccatgat
     3241 cgtctcgtcg aatcccggct ggggaattgc cgaggacgac ggccatggtg cgaccgatac
     3301 ggtggtgggg tatgcgccgg tcacggggct gagcctgccg gataactcct ggcacgtctt
     3361 cgtacggcaa caaccggagg aaacctacgc gccgattcgc gaccagttac ggaatctcgc
     3421 gttgatcggt ctggtcatgc tgtgtctgct ctgggccatg gggcggtatg tggcggcacg
     3481 gatcgcccgc ccgatccagg tgttgaaatc gggcgtggag gccatcagcc agggtacgta
     3541 cgatggtccg ctcgacatca aaacagggga tgagtttgag gagcttgccg tggccgtgca
     3601 ccggatggca gaccggttgc aatcctcacg aaccgaactg gaatcgttga atgcggagct
     3661 ggtgaatcgt gtggaggaga agacggcgga gattacccgt caaatgcgga aactggagtt
     3721 gtccgaacgc ctggccacgc tcggcaaagt cgccagcggc atcgcccatg agatcaacaa
     3781 tcccctcggg atcattttga accgggttga atgtatggaa gccgatgcgg cgcaggccgg
     3841 cgtgccggaa gaggtcggac gggatctggt gacgatccgg acgcaggcgg aacggatttc
     3901 acgggtcacg cgcagtatcc tcacgttctc ccgcggcacc gtctcgatgt tgaagcccct
     3961 ggatctcaac tgcgtggcgc gcacctgtgt cgctatggcg ggtgaacggg ttgcggcgct
     4021 ggcagtccgg attgacgcgc aactggcgcc cgaggtatca ccggtgatgg gcgaccgcga
     4081 tcgcctggaa acggtcctgc tcaatctgat caacaatgcc atcgatgcag tcaccggctc
     4141 cgacagtcaa gggattatca cggtgagcac tgcgcggatg ccgatcgacg gagacgagtg
     4201 ggtgcgaatc agcgtgtccg ataacgggcc cggtgtgccg accgagatcc tcgatcgcat
     4261 cttcgatccg ttctttacca ccaaaccggc cggtcaaggg accgggctgg gcctgtttct
     4321 gagttatgga attgtctcgg accatcgtgg ccgcattgag gtgcggaacc acgccgtcgg
     4381 cgccgtcttc gatgtgtatc tgcctgcggt ggcattcgga aatcgggttc atgagggaac
     4441 atcatgggga ttgcaggaaa aatcctgatc gtcgatgacg aacaggatgc gctggaaaat
     4501 tgccgtcgga ttttgagtcg tgtgccgtat gactgtctca ccgagtgtga tcccgcccgt
     4561 gcgctggatg tcattcagcg agagcggccc ggcctgattc ttacggattt gcgcatgccc
     4621 gatctcgacg ggattgaggt cctggcggca gccaaacggt tcgatccctc cgtgcaggtg
     4681 gtgctgttga cggctcacgc gaccgtgcag actgcggtca cgtcgatgcg gtatggcgct
     4741 ttcgattaca ttaccaagcc gtttacgagc gcagacctgg tgtcggttgc ccgtcgtgcg
     4801 ttcggggaag atggcgcggg gcctgtcgcc ggtgccgtcg gtacgcgcga gcttctcgaa
     4861 cctcctgagc aggcccgtcc cgggaagcgg cctggcttgt cacgagtcat cggcgagagc
     4921 cccgcgatgc actcggtgtt caatctgatt gaaaaggtcg cgccgacgca ttccaatgtg
     4981 ctggtctatg gagaaagcgg aacaggaaaa gagctcgtgg cgcgcgccat tcacgatgcc
     5041 agtctgcggg cggaccgtcc cttcattccg gtggattgcg tgtcgctgcc cgataccctg
     5101 ctggaatccg aactgttcgg ccatgaaaag ggggcgttta ccggcgccca tgtctcgaag
     5161 cccggcctct ttgaagtggc ggccggtgga accgtctttc tcgatgaagt cagcggcatg
     5221 agtcaaacct tgcagtcccg tctgttgcgg gtgttgcagg aacggcagat gcgacgggtg
     5281 ggcggcattc ggtttctgga ggtggatgtg cgggtgattg cggcctccaa ccaggatctg
     5341 gaatcggcct gtcgccgggg tgagttccgc gaggatctgt attaccggct gaacgtcatc
     5401 cccattgtgc tgccgccctt gcggactcgc gagggggaca ttcttctcct ggcgcgggag
     5461 tttctgcggc ggttcatgaa acagcagcga ctgggcctgg cgttcgtgcc ggagttgga
//
LOCUS       DAOQRM010000155         5512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_15151_length_5512_cov_15.601063, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000155 DAOQRM010000000
VERSION     DAOQRM010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15151_length_5512_cov_15.601063"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKK81_10920"
     CDS             <1..969
                     /locus_tag="PKK81_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNV33438.1"
                     /translation="TRGVFNLFKYDPGGRQMRFIASLTERIERKVLFDYVDPAFGNGQ
                     YSLNFGGTFFKNATSRFFGLGQATTQAAESNYTAREARAYWRLGLYANEVTQISVGQR
                     VRQVQLQRGAIDLPFSVEQFPTVDGIQGESIIVGHRASFYYDTRDSLVSPTDGVAITA
                     YAELNQNVKNGDHPVYSRYELEVKKLFPSESKRAILVVRADLQATIGSQVPFFEQSSL
                     GGQNNLRGFGLDRYIDKHLIAFSIEERIHILRTKLAGVTADFEVAPFLDTGQVFNSFK
                     DVSFQDYRMTPGLGFRAIVRPNVVGRVDYGYSREGGAVFAGLDFPY"
     gene            970..1998
                     /locus_tag="PKK81_10925"
     CDS             970..1998
                     /locus_tag="PKK81_10925"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HNV33439.1"
                     /translation="MPLSERPGLLLRYTIHCFCLLALLSVLPPAAGAEGFGPFPVRNF
                     QALDQLVLAMPGERATVLRKGDFDVRLEAANTASIARDSEAQAEVAMKFETIRAGLFL
                     RYGLTDRLELGAEIPGYHRYRGFMEGAIIGVERGTTGVAPPRKALQEAGYAFNITNGA
                     RTLFQGSEGATGLGDISFYGKYQILKETSSLPALSVRVGVKAPTGDTGEVFGSGHPDA
                     GIGLALDKKFADNWILYANLNGVFPTGRIAGLGLQPVVSGLVAVEYLWTENFSITAQF
                     DYYSPPFHGTGTRVLDKGVTESVLGVSYRILPGLLWQLYGVENLDFITGSAADFTLST
                     VLTYRFRS"
     gene            2182..4128
                     /gene="dxs"
                     /locus_tag="PKK81_10930"
     CDS             2182..4128
                     /gene="dxs"
                     /locus_tag="PKK81_10930"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNV33440.1"
                     /translation="MSLLKNIHSPADLKRLSPEQFPELCQEIREQILTVVSNVGGHLA
                     SNLGVVELTVALQYLLDTPKDKIVWDTSNQAYTHKLLTGRREQFHTLRQYGGLSGFCK
                     REESVYDTFNAGHAGTGVSAAFGMVEAREQRNEKHKVVCVVGDGAMTAGMTLEGLHHA
                     GGTNKDFIVVLNDNQMSISKNVGAISAYLNRTFTGEFYARMREETGQLLRKIPHIGLE
                     VQKIARRAEELAKGAILPGLLFEELGFQYAGPIDGHNFEHLLPTLENVLKMKGPVLLH
                     VITKKGLGYQAAMDNPVWFHACPPFVRETGVPAKKASRPSYTSMAIDALIKVAHQDKR
                     IVAVTAAMCEGTGLNAFEKVFPDRIYDVGIAEQHAVTFAAGMAAQGMKPVVALYSTFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDFAFLRHVPNMVVAAPKDE
                     NELQHMMKTCVTHDGPASVRYARGVSLGVPMDPEPTALPIGKGELLREGTDVAIVAIG
                     VTVWPAMKAAERLAQEGISAAVVNARFVKPLDTELIIKTAKNVRCLVTVEEGCKMGGF
                     GSAVLEALSEEGITNLRTKMIGLPDWYIEQGPQDLLRERYGLTADGIYNSVKALFGAS
                     VATDDAARLASLVGSLPHGDEQGS"
     gene            4173..5378
                     /gene="ispG"
                     /locus_tag="PKK81_10935"
                     /gene_synonym="gcpE"
     CDS             4173..5378
                     /gene="ispG"
                     /locus_tag="PKK81_10935"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNV33441.1"
                     /translation="MHITRKKTRQIQAGSVKIGGDAPISVQSMCSTDTRDVNATVAQI
                     RQLEEAGCEIIRVAVPDEAAAAALPQIKAAMTVPLIADIHFDHRLALKAAEVVDCVRI
                     NPGNIGAWWKVQEVIKAVNERGIPLRVGVNGGSLERPLLDKYGWPSPEALSESALNAV
                     HALEDEGFTNMKVSLKASDVHHAIDAYYLFSTQSNYPLHIGITEAGTAMTGAVKSAIG
                     LGWLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLGHIKTPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLMRKV
                     PLEELMDTLIHEVELLAKEKEAEAAGVTHETAASSDGQAEPGWELIGHAPDEHSTIVR
                     DIPVLPTKR"
BASE COUNT         1121 a         1470 c         1754 g         1167 t
ORIGIN      
        1 acacgcgggg tcttcaatct gttcaagtac gatccgggtg gacgacagat gcgattcatc
       61 gcctcgttga ccgaacgaat cgaacgcaag gtgttgttcg attatgtcga tccggcattc
      121 ggcaacgggc aatattcact caatttcggc ggcacctttt ttaagaacgc gacctcacgg
      181 ttcttcggcc tcggccaggc gacgacccag gccgccgagt ccaactatac ggccagggaa
      241 gcgcgcgcct actggcggct tggtctctac gcgaatgaag tgacgcagat ttctgtcggg
      301 cagcgggtcc ggcaggtgca gctccagcga ggagcgatcg atctgccctt ttcggtcgaa
      361 caatttccca cggttgacgg tattcaaggc gagtccatca tcgtgggcca tcgcgcctcg
      421 ttttattacg atacccgtga cagcttggtt tccccgaccg acggcgtggc cattaccgcc
      481 tatgccgaac tgaatcaaaa tgtgaaaaac ggcgaccacc cggtctattc gcggtatgaa
      541 ctcgaagtca aaaaattatt ccccagcgag tccaagcgcg ccattctggt ggttcgcgcc
      601 gacctgcagg cgacgatcgg atctcaggtg ccgtttttcg aacagtcttc gctcgggggg
      661 cagaacaatc tgcgcgggtt cggcttggac cggtacatcg acaaacatct gattgccttc
      721 agtatcgaag agcgaattca catcttgagg acgaaactgg ccggggtgac tgcggatttc
      781 gaggtggcgc cgtttctcga cacggggcag gtcttcaact cattcaaaga cgtcagcttt
      841 caagactatc ggatgacgcc ggggttgggg tttcgcgcca tcgttcggcc taatgtcgtc
      901 ggtcgggtcg attacgggta cagtcgcgag gggggagcgg tctttgccgg attagacttt
      961 ccctactaag tgccactgag cgaacggccg gggttactct tgcggtacac gattcattgt
     1021 ttctgcctcc ttgcactcct gagtgtcctg ccgcctgctg ccggagcgga aggcttcggt
     1081 ccgtttccag tcagaaactt tcaagcgctg gatcaattgg tcctggccat gccgggcgag
     1141 cgggcgacgg tgcttcgcaa gggcgatttc gatgtgcggc tggaagcggc gaatacggcc
     1201 agcattgcca gggacagtga agcgcaggcc gaggtggcga tgaagtttga aacgatccgg
     1261 gccggcctgt tcctccggta tggtctcacc gatcgattgg agctcggcgc agagattccc
     1321 ggctatcatc ggtaccgggg cttcatggag ggggcgatca tcggggtgga acggggcacg
     1381 accggcgtgg cgccgccgcg caaggctctg caggaggccg ggtacgcgtt taatattacg
     1441 aatggcgccc gcacgttgtt tcagggatcc gaaggcgcga ctggattggg agacatctcg
     1501 ttctacggca agtaccagat tctcaaagaa acctcgagcc ttccagccct gtcagtccgg
     1561 gtcggggtca aggctccgac cggagacacc ggcgaagtgt tcggcagcgg gcatcctgac
     1621 gccggtatcg gcttggcgct ggataagaag ttcgccgata actggatcct gtacgcgaat
     1681 ctcaacgggg tgtttcccac gggacggatt gccgggttgg gactccagcc ggtggtgagc
     1741 gggttggttg ccgtggaata tctctggacg gagaactttt ccatcacggc acagttcgat
     1801 tattattcac cgccgtttca cgggacaggc acgcgcgtcc tggataaggg cgtgacggag
     1861 tcggtgctgg gggtgagtta tcgaatcctt cccggtctgc tctggcagct gtatggagtg
     1921 gagaatctgg atttcatcac cgggagcgcg gcggatttta ccctctccac cgtcctcacc
     1981 taccggttcc ggtcgtagtc gcgagggcga agtttttggt gcaggggaag gcgaaatctt
     2041 gacacaggca gtgccatatg tgaggatgag ccgattatac gaaactgctt cgctgcttcg
     2101 tcgcgacctt ctctatacgg cttgttccca ctgaatgaat acctgcgccc aattgtcatc
     2161 atttgacaag gagattgtgc aatgtctctg ctgaaaaata tccacagtcc cgctgatttg
     2221 aaacgcctct cccctgagca gtttccggaa ctgtgccagg agatccgtga gcagatcctt
     2281 acggtggttt cgaatgtcgg ggggcacctg gcctccaatc tgggtgtggt ggagctcacg
     2341 gtcgcgctgc aatatctcct ggatacgccg aaagacaaaa tcgtgtggga taccagcaat
     2401 caggcctata cccacaaact gctcaccgga cgacgggagc agttccacac gctccggcag
     2461 tacggcgggt tgagcgggtt ctgtaagcgc gaggagagtg tctatgatac cttcaatgcc
     2521 gggcatgccg gcaccggagt ctccgccgca ttcggcatgg tggaggcgcg cgagcagcgg
     2581 aacgaaaaac acaaagttgt ctgcgtggtg ggtgacggcg ccatgacggc cgggatgacc
     2641 ctcgaagggt tgcaccatgc cggcggcacc aacaaggatt tcatcgtcgt cctgaacgac
     2701 aaccagatgt ccatctcgaa gaatgtcggg gcgatttcgg cctacctgaa ccggaccttc
     2761 accggcgaat tctacgcgcg tatgcgggag gaaaccggcc aactgttgcg gaagattccc
     2821 cacatcggcc ttgaggtgca gaaaatcgcc cgacgggctg aggaattggc caagggggcg
     2881 attctccccg gtctgttgtt tgaggaattg ggcttccagt atgccggacc gatcgatgga
     2941 cataatttcg agcacctgct gccgaccttg gagaacgtgc tgaaaatgaa gggcccggtc
     3001 ctgctgcacg tgattacgaa gaagggcttg ggctatcagg cggccatgga taatccggtc
     3061 tggttccatg cctgtccgcc ctttgtgcgt gaaacgggtg tgccggccaa gaaagcatcg
     3121 cgccccagct atacgagtat ggcgattgat gcgctgatca aagtcgctca tcaggacaag
     3181 cggatcgtgg ctgttaccgc cgccatgtgc gagggcacgg gcctcaatgc gttcgagaag
     3241 gtgttcccgg atcggattta tgacgtgggt atcgccgagc aacatgctgt caccttcgcc
     3301 gccggtatgg ctgcgcaggg catgaagccg gtggtcgcgc tctattccac attcttgcag
     3361 cgggcctatg accaggtggt gcacgatgtg gctactcaga acctgccggt gaccttctgc
     3421 atcgaccggg gcggtctggt ggccgaggat ggtacgacgc accacggcgc gttcgatttt
     3481 gccttcctgc gccatgtgcc gaatatggtg gtggcagcac cgaaggacga aaacgagctg
     3541 caacacatga tgaagacctg tgtgacgcat gacgggcccg cctcggtgcg gtacgcgcgc
     3601 ggcgtgagtc tcggggtgcc gatggatcca gaaccgacgg cgttgccgat cggcaagggc
     3661 gagttgttgc gggaaggcac ggatgtggcg attgtggcga tcggagtcac cgtctggccc
     3721 gccatgaagg cggctgaacg gttggcgcag gaggggatct ccgctgcggt ggtcaacgcc
     3781 cgtttcgtga agcccctgga tacggaattg atcatcaaga cggcgaagaa cgtgcgctgc
     3841 ctggtgactg tcgaagaagg ctgcaagatg ggtggattcg gttccgcggt cttggaagct
     3901 ttgtccgaag aaggtatcac gaacttgaga acgaagatga tcggcctgcc cgattggtac
     3961 atcgagcagg gtccgcaaga cctgcttcgc gaacggtatg gcctgacggc agacggaatt
     4021 tacaacagcg tgaaggcctt gttcggggcc agtgtggcca cggatgatgc cgcccggttg
     4081 gcgtcgctgg tggggagttt gccgcacggc gatgagcagg gcagttagtt gacgtcgggg
     4141 cgcaccaaaa caggacatac gctgagaagc tgatgcatat cacgagaaaa aagacgcggc
     4201 agattcaggc gggatcggtc aagatcggcg gagatgcgcc gatttcagtc cagtcgatgt
     4261 gctcgacgga tacacgcgat gtgaacgcca cggtggctca gatccgtcag ctggaagagg
     4321 ccgggtgtga aatcattcgt gtcgccgtcc ctgatgaggc ggcggcagct gcgcttcctc
     4381 agatcaaagc cgcgatgacg gttccgctga tcgccgacat tcacttcgac catcgcttag
     4441 ccctcaaggc ggcggaagtg gtggattgcg tccgcatcaa tccaggcaac atcggcgctt
     4501 ggtggaaggt gcaggaagtc attaaggccg tcaatgagcg gggcattccc ctgcgcgtgg
     4561 gcgtgaacgg cggctcgctc gaacgtccgt tgttggacaa gtatggttgg ccttctcccg
     4621 aagccctgtc tgagtcggct ttaaatgccg tccatgcctt ggaagacgag ggcttcacca
     4681 atatgaaggt gtcgctcaag gcttccgacg tgcaccatgc catcgatgcc tattatttgt
     4741 tctccaccca gtcgaattat ccgctacata tcggaatcac ggaagcgggc acggcgatga
     4801 cgggtgcggt caagtccgca atcggcctcg gttggctgct ctcgcagggc atcggggata
     4861 cccttcgagt ctcgttggcg gctgatccgg tggaggaagt caaagtcggg ttcgagattc
     4921 tcaagtcgct ggagttgcgc catcgtggca tcaatgtgat tgcctgtccg acctgcggcc
     4981 gggttgagat cgatgtggtg cgcatggcga atgaactgga aaagaagctc ggccatatca
     5041 agacgccgct gaacgtctcc gtgctcggct gtgtcgtgaa cggcatcggg gaaggcaagg
     5101 aagcggatat cggcatcgcc ggcggtgaag gcaaagggat tctcttcaag aagggcaaat
     5161 tgatgcggaa agtgccgctt gaagaactca tggacacgct gatccacgag gtcgagttgc
     5221 tggccaagga aaaagaagcc gaggccgctg gagtgactca cgaaacggca gcatcgtcag
     5281 atgggcaggc ggagcctggg tgggaattga tcggtcatgc gccggatgag cattccacca
     5341 tcgttcgcga tattcctgtt ttgccgacga aacgctgatc agaagcggat ccccctcagt
     5401 tggactatgt tttgaacatt cctccatttc tctgctcttc gcggttgagt tcctgaagct
     5461 cccccttcta taatccgatc cgtggcgccg tacccaagtg gctaagggag ca
//
LOCUS       DAOQRM010000156         5472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_15309_length_5472_cov_21.832380, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000156 DAOQRM010000000
VERSION     DAOQRM010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15309_length_5472_cov_21.832380"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..2921)
                     /locus_tag="PKK81_10940"
     CDS             complement(36..2921)
                     /locus_tag="PKK81_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HNV33442.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRR
                     AHNRFGFALQLCVLRYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHE
                     HLAELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATS
                     TIERLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGS
                     NSSSANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLA
                     ILAVCVSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIG
                     GALVDAQDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRY
                     APRMLRLLDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDAR
                     LWEIAVLFHLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLAD
                     RQARLDMRLRELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPT
                     RITDLLLEVDAATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHT
                     FWELIRIGRWHVEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEA
                     MNLVNAKYGNTPGLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQ
                     FTDTGGFTDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAM
                     IERNWPDILRIAATIAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWI
                     LDAELQRRAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIF
                     WNTMKLGEVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
     gene            3047..3661
                     /locus_tag="PKK81_10945"
     CDS             3047..3661
                     /locus_tag="PKK81_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNV33443.1"
                     /translation="MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRD
                     DRPGLTACLKSLRDGDVLVVWKLDRLGRSLAHLVNTVKELSDRKIGLRVLTGKGAQID
                     TTTASGRMVFGIFATLAEFERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQA
                     AMAQRDTSVSDLCKELGIERVTLYRYVGPKGELRDHGKHVLGLT"
     gene            3727..4530
                     /gene="aph(3'')-Ib"
                     /locus_tag="PKK81_10950"
     CDS             3727..4530
                     /gene="aph(3'')-Ib"
                     /locus_tag="PKK81_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HNV33444.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            4530..5366
                     /locus_tag="PKK81_10955"
     CDS             4530..5366
                     /locus_tag="PKK81_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HNV33445.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT         1084 a         1589 c         1658 g         1141 t
ORIGIN      
        1 aaggggtcgt ttgcgggagg gggcggaatc ctacgctaag gctttggcca gcgatattct
       61 ccggtgagat tgatgtgttc ccatccgagc ggcgaaacat gggccaagag atcgggcgat
      121 agcagctttc catcgcgttt ctggtttgca acgacctcgc cgagcttcat ggtgttccag
      181 aagatgatga tggcggcgag cagattcatg ccggcgatgc ggtaatgctg gccttcggcg
      241 gaacggtcgc ggatttcacc gcggcggtgg aagctgattg cccgcttcag cgcatgatga
      301 gcttcgcctt tgttgagccc gatctgggca cgccgttgga gttcggcatc cagaatccag
      361 tcgatcatga acagggtgcg ctcgacgcga ccgacttccc gcagggctgt cgcgagctcg
      421 ttctgccgcg gataggaggc gagtttccgc agaatctggc ttggcgcgac ggtcccggca
      481 gcaatggtgg cggcgatgcg caggatgtcg ggccaattgc gctcgatcat ggcttggttg
      541 acctttccgc cgatcaacgc tcgcaggtgc gccggggcgg ccgacggatt gaacgcgtag
      601 agccgtttgg atggcaggtc gcggatgcgc ggagcgaacc ggtagccgag aatggcacat
      661 gcggcaaaga cgtgatcggt gaagccgccc gtgtcggtga actgctcgcg gatatggcgt
      721 ccagcatcgt tcatcagcag gccatcgagg atgtaaggcg cttcgcttgc cgttgcagga
      781 atcacctggg ttgcgaacgg cgcatattgg tcggagacgt ggctataggc tttcaggccc
      841 ggggtattgc catatttcgc gttgaccagg ttcatggcct caccttgctc tgtagcgacg
      901 aagaactgtc cgtcgctcga agccgacgtg cccatgcccc agaaccgggc catgggtaac
      961 gctgcctgtg cctcgaccac catggccagc gcccggtcat aggcttcgcc ctcgacatgc
     1021 caccgtccaa tgcggatcaa ttcccagaag gtgtgggtgt ttgtcgcatc cgccattttg
     1081 cgcaagccga ggttgatccc ttccgccaag ataacgttca ttagcccgat ccggtcagcg
     1141 cagggtgctc ctgtgcgcag atgggtgaac gcttcggtga agccggtcgc cgcatccacc
     1201 tccagcagga gatcggtgat gcgcgtgggc gggatctgct tgtagagatc gagcaccaga
     1261 tcttcggcgc ctgtcggcgc ggcggcttcg agtttctcga tatgcagaac gccgttttca
     1321 atcgacccgc ccgggatcgt gcctgcgcga gcggcacggc caagctcgcg caaccgcatg
     1381 tcgaggcgag cttgccggtc tgccagccat tcctccggcc gcaatggcac agcgagacga
     1441 ccgccttccg cgatggattg tgccggaacg agtgcgtgtt tcagatcgcc atagcgccgg
     1501 gacctagtaa gccagacatc tccggagcgg aacgcatcgc gcagatggaa cagcaccgcg
     1561 atctcccata ggcgagcgtc gccagccctc tgggcccgaa ggtggcgatg ccatttcgag
     1621 ctgggccgca agaagctggt catcgcggca tcgttcaaac cggtacgaag ggccgtcacc
     1681 gcttccagaa gcggcagtgc aacgggcgca gctcgcagat cgagcaggcg caacatgcgt
     1741 ggagcgtatc ggcggaagcg gtgataaccg tcgagcacat gattgagcgg atcgtcggcc
     1801 atggtggcgg tcagcctggt tgccattgca acaagggttt ttaagccgtc ccaccctgac
     1861 ccactcgcga tgacatcgcc cagcggctgg ccatcatcct gtgcatcgac cagggcgccc
     1921 ccgatctcgg cgaaggattt cagggtgtca cgcaccaccc ccgcttcgtc tgcgaccttt
     1981 gcatggcaaa tacgctccga agcacggtag agacggccga cgatccggtc gtgggtttcg
     2041 accactgcgt cggccaacat cgcctgccat tccgagacgc aaacagccaa gatcgcaagc
     2101 cgcctgtcct ccgggagatc gcgcatgccg tcggcataat accgttcacc ctgcctgcgc
     2161 agacgagtca cccgatgggc aggaacgccg gcaagcagat cctcggggag atcgatgcgt
     2221 tgcagatatt cgagccggtc gagcagccgg ttggccgacg aagagttcga gccaggctcg
     2281 aactggcgca gccacacaaa acgggtcacc cgatcatcag ccgtctcctc gagcaatgcc
     2341 agcaactgtt ctcggatcga cataggcagc cgactggcga tcctcgtctc gatgcgtcgc
     2401 tcggcatcga cgagagccgc ggcacaaagc cgctcgatcg tggatgtcgc gggaaggaca
     2461 gtgcgggtgc gtcggcactc ggctacgaag cgacgggcga tatcctcgtt cgacaccgcc
     2521 atctcggctt ctcggaacaa ccattccttc agctcgctcg caccacgtcc ggagaaggtg
     2581 cggaagccgt agagcccccg taactcggca agatgctcgt gccgtgtttc ctcgcgggca
     2641 gcatagtcta cgagatcgtc ggcacccagg ccaagctgcg ctccgataaa ttcgatgacc
     2701 tctgcaggga tcagttcgcc tggagccagc acccggccgg gatagcgcag gacacacaat
     2761 tgcagggcga agccgaacct gttgtgagcg cgccgacgca gcctgatatg cccaaggtct
     2821 tcatcactca gcgtatagtg cttgagcaaa tccgtctgtg aagtcggcaa gcgcaacagc
     2881 gcgtctttct gccgatcggt tagagtgacg cgacgcggca tacatgttcc tttttcaaaa
     2941 tctgatagcg ttcaagacgc tttgtttatg aagctggttg agatacattt ccagaggtca
     3001 atgcaatcgt ggccgaagcg ccgcctcaaa ccaacgtttg tgatacatgc tgatcggata
     3061 tgcccgcgtc tccaaagccg atggctcgca gtctctcgac ctgcagcacg acgccttgcg
     3121 cgccgcaggt gtcgaacggg acaatatcta tgatgatctt gcttccggcg gtcgtgatga
     3181 tcgccctggc ttgactgcct gcctcaagtc attgcgtgac ggcgatgtgc tggtggtctg
     3241 gaagctcgat cgcctcggac gatcgcttgc ccatctggtc aacacggtga aggagctgtc
     3301 agaccgcaag atcggcctgc gggttctgac tggaaagggc gctcagatcg acaccacgac
     3361 tgcgtccggt cgcatggtgt tcggaatctt cgccaccttg gccgagttcg agcgggatct
     3421 gatccgagag cgcaccatgg cgggtctcgc ctccgcgaga gcgcgcggtc gcaagggcgg
     3481 acgaaaattc gcgctcacca aagctcaggt gcgtctcgcg caagccgcca tggcccagcg
     3541 cgatacttca gtttccgatc tctgcaagga actcggcatc gagcgcgtca ctctctaccg
     3601 atatgtcggt cccaaaggcg agctcagaga ccatggaaag catgttctcg gacttacgta
     3661 gcaactcgtt tcttttcgca ggttgagcca cctccgcgct tcatcagaaa actgaaggaa
     3721 cctccattga atcgaactaa tatttttttt ggtgaatcgc attctgactg gttgcctgtc
     3781 agaggcggag aatctggtga ttttgttttt cgacgtggtg acgggcatgc cttcgcgaaa
     3841 atcgcacctg cttcccgccg cggtgagctc gctggagagc gtgaccgcct catttggctc
     3901 aaaggtcgag gtgtggcttg ccccgaggtg atcaactggc aggaggaaca ggagggtgca
     3961 tgcttggtga taacggcaat tccgggagta ccggcggctg atctgtctgg agcggatttg
     4021 ctcaaagcgt ggccgtcaat ggggcagcaa cttggcgctg ttcacagcct atcggttgat
     4081 caatgtccgt ttgagcgcag gctgtcgcga atgttcggac gcgccgttga tgtggtgtcc
     4141 cgcaatgccg tcaatcccga cttcttaccg gacgaggaca agagtacgcc gcagctcgat
     4201 cttttggctc gtgtcgaacg agagctaccg gtgcggctcg accaagagcg caccgatatg
     4261 gttgtttgcc atggtgatcc ctgcatgccg aacttcatgg tggaccctaa aactcttcaa
     4321 tgcacgggtc tgatcgacct tgggcggctc ggaacagcag atcgctatgc cgatttggca
     4381 ctcatgattg ctaacgccga agagaactgg gcagcgccag atgaagcaga gcgcgccttc
     4441 gctgtcctat tcaatgtatt ggggatcgaa gcccccgacc gcgaacgcct tgccttctat
     4501 ctgcgattgg accctctgac ttggggttga tgttcatgcc gcctgttttt cctgctcatt
     4561 ggcacgtttc gcaacctgtt ctcattgcgg acaccttttc cagcctcgtt tggaaagttt
     4621 cattgccaga cgggactcct gcaatcgtca agggattgaa acctatagaa gacattgctg
     4681 atgaactgcg cggggccgac tatctggtat ggcgcaatgg gaggggagca gtccggttgc
     4741 tcggtcgtga gaacaatctg atgttgctcg aatatgccgg ggagcgaatg ctctctcaca
     4801 tcgttgccga gcacggcgac taccaggcga ccgaaattgc agcggaacta atggcgaagc
     4861 tgtatgccgc atctgaggaa cccctgcctt ctgcccttct cccgatccgg gatcgctttg
     4921 cagctttgtt tcagcgggcg cgcgatgatc aaaacgcagg ttgtcaaact gactacgtcc
     4981 acgcggcgat tatagccgat caaatgatga gcaatgcctc ggaactgcgt gggctacatg
     5041 gcgatctgca tcatgaaaac atcatgttct ccagtcgcgg ctggctggtg atagatcccg
     5101 tcggtctggt cggtgaagtg ggctttggcg ccgccaatat gttctacgat ccggctgaca
     5161 gagacgacct ttgtctcgat cctagacgca ttgcacagat ggcggacgca ttctctcgtg
     5221 cgctggacgt cgatccgcgt cgcctgctcg accaggcgta cgcttatggg tgcctttccg
     5281 cagcttggaa cgcggatgga gaagaggagc aacgcgatct agctatcgcg gccgcgatca
     5341 agcaggtgcg acagacgtca tactagatat caagcgactt ctcctatccc ctgggaacac
     5401 atcaatctta ccggagaata tcgttggcca aagccttagc gtaggatttc gccctctccc
     5461 gcaaacgacc cc
//
LOCUS       DAOQRM010000157         5389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_15659_length_5389_cov_11.876453, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000157 DAOQRM010000000
VERSION     DAOQRM010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15659_length_5389_cov_11.876453"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKK81_10960"
     CDS             <1..427
                     /locus_tag="PKK81_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV33446.1"
                     /translation="QAVLSQGADVNGVDESGNTALIYAACYGEGEAIEYLLSKGADVN
                     QQAQDGETPLIAASYGCHAKSAEILLAHGADCNMLYQHGWTALATAVVQGCNDSIQPL
                     LAAGSDPNKEVAEGYPPLIVALAKGHTAIVRTLLAGVRM"
     gene            complement(1192..1521)
                     /locus_tag="PKK81_10965"
     CDS             complement(1192..1521)
                     /locus_tag="PKK81_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNV33447.1"
                     /translation="MMHHHAEPGPDKIPVRIDSSLEAIIPGFLANRQRDLTTIESCLK
                     QGDLNTIRMLGHRMKGDGGGYGFDQISVIGDQLERAAMAHNLSALHDQLNGLKDFLAR
                     VEIVYVH"
     gene            complement(1518..>1769)
                     /locus_tag="PKK81_10970"
     CDS             complement(1518..>1769)
                     /locus_tag="PKK81_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV33448.1"
                     /translation="HYDLILMDMQMPVMDGITATRTIRRWEQEQGLPAVQIIALTALA
                     LKEESARIFEAGCNAHMTKPLKRTTLLELLLAYEKTRAQ"
     assembly_gap    1770..1869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1870..3146)
                     /locus_tag="PKK81_10975"
     CDS             complement(<1870..3146)
                     /locus_tag="PKK81_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV33449.1"
                     /translation="MAKRITQTRRTTSTSATRLPRTASRPSTALQREQAARLRAERAL
                     QRVSKELQRRVEELQRAKDAADVANRAKSEFLASMSHEIRTPMNAIIGMADLLWETTL
                     TPEQRKYLRIFRRAGASLLSLINDILDLSKVESGRLDLDSIDFDLSELIDKTSEMLAL
                     RANEKGLELVCHLANDVPCHLIGDPNRLYQILLNLLGNAIKFTEKGSVVMSITNDPEL
                     RTPGAIRFSISDTGIGVPHDKIKAIFDTFTQAHSTIARQYGGTGLGLSISQQLAALMG
                     GRIWAESTLGEGSTFHCVVQLGVQTAPPPSKVSGTMNLAGLRCLVVDDYPTNRLILRE
                     TLFGWGATVVEADSGEQAMEALEVSLKSDAPFTLLLLDCRMPGMDGFEVIERIHADPR
                     LSNLTIIMLASDRWADDIAKTYDLGLGGYLVKP"
     gene            complement(3229..4233)
                     /locus_tag="PKK81_10980"
     CDS             complement(3229..4233)
                     /locus_tag="PKK81_10980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV33450.1"
                     /translation="MGILIVDDSTDDRLLIQSILANAGYEETFGAESAAAAFRLLGLD
                     GPRQMTGRVDLILMDIVMPATNGIEACRQIKAVERYRDIPIIMVTVKTDPVDLQLAFA
                     AGAIDYVAKPISKIELLTRVRCVLRLVHEIERRRAREQELLEVMRQLQEANQMLLRLS
                     CLDGLTGITNRRQFDDFLDQEWRRAARESTPLSLIMLDIDYFKTYNDSHGHQAGDECL
                     RQVAQLISSHVNRPGDLVARYGGDEFVIVLPGTTVDGAAQVADTLRRKVCEAEMKHPN
                     GDPVTISLGFACTVPSRVAAPTDLIRAADQALYQAKQDGRNRAKQAGVLTLVHHTHKD
                     "
     gene            complement(4278..4925)
                     /locus_tag="PKK81_10985"
     CDS             complement(4278..4925)
                     /locus_tag="PKK81_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV33451.1"
                     /translation="MGILIVDDSPDQQLLLKAVLNKAGHQDLLIADSAASAYDHLGIN
                     QPQPNGQAPAIDLILMDFLMPGIDGVAATQRIKSLENLRDIPVIVVTAKTALGDLQAA
                     FAAGAMDFITKPVNSVELLARVNSALMLKKEMDCRKTREMELRRSNAELQQALREVKV
                     LKGLVPICASCKKIRNDQGFWQQLEEYIQQHSEAEFSHGLCTPCIKKHYPGVYPD"
     gene            complement(5094..>5389)
                     /locus_tag="PKK81_10990"
     CDS             complement(5094..>5389)
                     /locus_tag="PKK81_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNV33452.1"
                     /translation="MTTLIKQRKEAAEQFEKGNRQDLATKERQEISIIEGYLPAALSS
                     DELARIVDAAVKESGATALKDMGQVMKAVMARLAGQSVDGKVVSDLVKAALQR"
BASE COUNT          999 a         1459 c         1667 g         1164 t
ORIGIN      
        1 gcaggctgtc ttgagtcagg gcgccgatgt gaatggagtc gacgagagcg ggaataccgc
       61 attgatctat gcagcttgtt acggagaagg agaggcgata gagtatctac ttagcaaggg
      121 ggctgatgtc aaccagcaag cccaagacgg tgagactcca ttgatcgcag catcgtatgg
      181 atgccatgcg aagagtgccg agatacttct ggctcatgga gctgactgca acatgctgta
      241 tcagcatggc tggacagctc tggcgacggc agtcgtgcaa ggctgcaatg acagcatcca
      301 gcccctactt gcagctggtt ccgatcccaa caaagaggtt gcggaaggat atcctccttt
      361 gatcgtcgct cttgctaaag gtcacactgc cattgtgcgg acactccttg ctggagtgcg
      421 gatgtgaatt ataaggggag caaaggcatt actccctcat cgtggcatca agcctcggga
      481 atcgtgaaat ggtgcaagtg ttactcaatt atggagccgc agtaaactca cgagtcattg
      541 ctggacagga caaggctttt aaaaccggtg acacttcgct gaaagttgct cggcggaatt
      601 accattcgga tattgagcaa attctcatag cagcaggtgc tgtggaatag aaatacggct
      661 aggtagcgaa agaggtttcc tttccaacga ttcagaccgt cccgaggctg cgaagtcttc
      721 acagttgtag tcacagtggc tcatggactt ggcgctcaaa tacagcccat agagctaagc
      781 agcaaatgca acgcccgcaa ttttaaacga aatagctttt gccaacgatg ggcgtagcgg
      841 ttgccctcca ccaaacctcg tctgtgcgat ccgaggcgag tagggtagtt cctcccgtcc
      901 tgatcaaacc cggcgcatct ccttgggtga ccccgcatag ccttctttct cgccccagtt
      961 ttcgtgacca atcctcctgc tcgcgctcgg ctgcaacctt cggtctccgt ctctcatgga
     1021 attccgcaca tatcttccac agccctcatt cctcgaaact gccccagtga gcctgcattg
     1081 tcgccggata tgtttggctt gtgtcgtcta caatttagcg ggtagagcag tctgtaagag
     1141 agcaggtcgc gcaaatggag gaaaggggag cgacgggcga gggcactgaa ctcagtggac
     1201 gtacacaatc tcgacgcgag ccaggaagtc cttcaaaccg ttgagctgat cgtgtagcgc
     1261 cgagagattg tgtgccatgg cggcccgctc aagttggtcg ccgatcacgc tgatctgatc
     1321 gaaaccgtac ccgccgccgt cgcctttcat gcgatggcca agcatgcgaa tcgtgttgag
     1381 atcgccctgc ttcaggcagg actcgatcgt ggtgagatcc cgctgccggt tggcgaggaa
     1441 cccggggatg atggcttcaa ggctggaatc gatacggacg gggatcttgt cggggcctgg
     1501 ttcagcatga tggtgcatca ttgcgctcgc gtcttttcat aggccaggag taattccagc
     1561 agcgtggttc gtttcagcgg ctttgtcata tgggcattgc acccggcttc gaagatcctc
     1621 gccgattctt ctttcagagc caaggctgtg agggcaatga tttgaactgc gggaaggccc
     1681 tgttcctgct cccaacgtcg aatggttcgt gtcgcggtga tgccgtccat gaccggcatc
     1741 tgcatatcca tgaggatgag gtcatagtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnna tcggtttgac caggtagccg cccaggccga ggtcgtaggt cttggcgatg
     1921 tcgtcggccc accggtcgga ggcaagcatg atgatcgtga ggttgctcag cctcggatcg
     1981 gcatggatgc gttcgatgac ctcaaacccg tccattccgg gcatccggca atcgagcagg
     2041 agcagggtga acggggcgtc ggacttcagc gagacttcca aggcctccat ggcctgctcg
     2101 ccgctgtcgg cttccaccac ggtcgcaccc cacccgaaca gggtttcgcg caggatcagc
     2161 cgattggtcg ggtaatcgtc cacgaccagg cagcggagtc cggccaggtt catcgtgccc
     2221 gacactttcg atggcggcgg ggctgtttgc acgccaagct ggaccacgca gtggaacgtg
     2281 ctgccttcgc cgagggtgct ttccgcccag atccggccgc ccatgagggc tgccagctgt
     2341 tgcgagatcg acaagcccag ccccgtgccg ccgtactggc gggcaatcgt gctgtgcgct
     2401 tgcgtgaacg tgtcgaagat ggctttgatc ttgtcgtgcg ggacgccgat gcccgtatcg
     2461 ctgatcgaga atcgaatcgc gcccggcgtt cgaagctcgg gatcgttggt gatactcatg
     2521 accaccgagc ccttctccgt gaatttgatc gcgttgccca gcagattgag caggatctgg
     2581 tagagacggt tgggatcgcc gattagatgg caggggacat cgttggcgag gtggcagacg
     2641 agttcgaggc ctttctcgtt ggcacgcaag gccagcatct cactggtttt gtcgatcagc
     2701 tcggacaggt cgaagtcgat cgagtccagg tccaggcggc ctgattcgac tttcgagagg
     2761 tcgaggatgt cattgatcag gctcagcaga ctggctccgg cgcggcgaaa gatacgcaga
     2821 tatttccgtt gttccggagt gagggtcgtt tcccataaga ggtcggccat gccgatgatg
     2881 gcattcatcg gggtgcggat ctcgtggctc atgctggcca ggaactcgct cttggcacgg
     2941 ttggccacat cggcggcatc tttggcccgt tgcagttctt cgacgcggcg ttgcaattcc
     3001 ttggacacgc gctggagcgc ccgctcggca cgcaggcgcg cggcctgctc gcgttgcagc
     3061 gcggtggacg gtcgggaagc ggtgcgggga aggcgtgtgg cagaggttga ggtggttcgt
     3121 ctggtctgcg tgatgcgttt cgccatggcg tggtccggtc gtctcagtcc agcatgaccg
     3181 ctccgtctcg cgagtgggtg acacgatggt cgcgacggcg ggaggtcatc aatctttatg
     3241 cgtgtggtgc acgagcgtca ggactccggc ttgcttggcc cgattgcgcc cgtcctgctt
     3301 ggcctggtac agggcctggt cggccgccct gatgagatcg gtcggcgcgg ccacacggct
     3361 tggcacggtg caggcaaagc cgagactgat ggtgacggga tcgccgttcg gatgtttcat
     3421 ctccgcttca cagactttgc gccggagtgt atcggcgacc tgcgccgcgc cgtctactgt
     3481 ggtgccgggc agcacgatga cgaactcgtc gcctccgtaa cgggcgacca agtcaccggg
     3541 gcgattgacg tggctggaga tgagctgggc cacctggcga agacattcat ccccggcctg
     3601 gtgcccatgg ctgtcgttgt atgtcttgaa gtagtcgata tcgagcatga tcagcgacag
     3661 cggggtcgat tcgcgcgcgg cccggcgcca ttcctggtcc agaaagtcgt cgaactggcg
     3721 tcgattggtg atcccggtga gcccgtcgag gcacgagaga cgcaacagca tctggttggc
     3781 ttcctgcaac tggcgcatca cctccaggag ttcctgctcg cgcgcgcggc gccgttcgat
     3841 ttcatggacg aggcggagca cgcaccggac gcgggtcagg agctcgatct tgctgatggg
     3901 cttggcgaca tagtcgatgg cgccggccgc aaaggccaac tgcagatcga ccggatccgt
     3961 ttttacggtg accatgatga tcgggatgtc gcgatagcgt tcgacggctt tgatctgccg
     4021 gcaggcctcg atcccgttgg tggcaggcat gacgatatcc atcaggatca gatccactcg
     4081 gcccgtcatt tgccgcgggc cgtcgagccc gagcaaccgg aatgccgcgg cggcggactc
     4141 cgccccaaag gtttcttcgt aaccggcatt ggcgaggatg gattgaatca acagacgatc
     4201 gtccgtcgag tcgtcgacga tcaggatacc catggtcagt gtctccctga aagtgtgtcg
     4261 taggacgatg ctagcagcta gtcggggtaa acgccagggt aatgcttttt aatgcagggg
     4321 gtgcagaggc cgtgactgaa ttcggcttcc gaatgttgct ggatatactc ttccaattgt
     4381 tgccagaacc cctgatcgtt ccggattttc ttgcaggagg cacagatcgg caccaggcct
     4441 ttcagcactt ttacttcgcg caacgcctgt tggagttcgg cattgctgcg gcgaagctcc
     4501 atttcacggg ttttgcgaca gtccatttct ttcttgagca tcagcgcgga attcacccgg
     4561 gcgagcagtt ccacactatt gaccggcttc gtgatgaagt ccatcgcgcc ggccgcgaaa
     4621 gccgcctgaa gatcccccag ggcggtcttc gccgtgacga cgatcacggg gatatcccgc
     4681 aggttttcca ggctcttgat acgctgtgtg gcggccaccc cgtcgatgcc cggcataaga
     4741 aagtccatca ggatcaaatc aatggcgggt gcctgcccgt tcggctgggg ctgattgatg
     4801 ccaaggtggt cgtaggctga ggcggccgag tccgcgatga gcaggtcctg atgaccggct
     4861 ttgttcagca cggctttcag cagcagctgt tggtcgggag aatcgtcaac gatcaggatg
     4921 cccatgtgca gcctcgtccg cttggtggcg tgtgagcagg tgcgatgcct gccgatcggg
     4981 catgttgaat gcgagacata cccgggatgg catcggcgtg atgccacccg tcaggagccg
     5041 tatcggcaga atcgaggaag agattaagag tggtggtgcc ggagccggca catttaacgt
     5101 tgaagagcgg ctttgaccag atcgctgacg actttgccgt ctacggattg gccggccaag
     5161 cgggccatga cggctttcat cacctggccc atgtctttga gggccgtagc gccggattcc
     5221 ttcaccgcgg cgtcgacgat ccgggccagt tcatcggagg agagcgcggc ggggagatac
     5281 ccctcgatga tgctgatctc ctgccgttct ttcgtcgcca ggtcctgccg gttgcccttt
     5341 tcgaattgct cggcggcttc cttgcgttgc ttgatgagcg tggtcatga
//
LOCUS       DAOQRM010000158         5380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_15703_length_5380_cov_17.620094, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000158 DAOQRM010000000
VERSION     DAOQRM010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15703_length_5380_cov_17.620094"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /gene="holB"
                     /locus_tag="PKK81_10995"
     CDS             complement(<1..632)
                     /gene="holB"
                     /locus_tag="PKK81_10995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNV33453.1"
                     /translation="MPFADIIGHDSAKTLLRSAILQNRLAHAYLFHGDDRIGKRLLAL
                     RLAQALLCETVPDDPTPDACGACRACRQVDARTHPDFMVIEPDQELANPQIKIELIRD
                     IEHQMIYRPLIGDRKICLIDDADRMTIGAANALLKTLEEPPDHSLFILVSSRPYALPA
                     TIRSRCQALRLTAPAQTQVEAAVILKRELPPADAHFLAVLSDGQLGRALE"
     gene            complement(619..1347)
                     /gene="tmk"
                     /locus_tag="PKK81_11000"
     CDS             complement(619..1347)
                     /gene="tmk"
                     /locus_tag="PKK81_11000"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNV33454.1"
                     /translation="MMTPRSRPPRGLFITLEGTEGCGKSTQAKLLGEHLRNQGYDTVE
                     TREPGGTPLAEKIRSLLLDRADEPVAPETEAFLVLAARRQHVAQVIEPALARGSIVLC
                     DRFIDSTLAYQGYARGLDIATLERLNRLATHALMPDLTLVFDLPVSTGLARRRSAAEL
                     NRLDRESLRFHRSVRTGFLDLAKRHPTRIKVLSARASKETVARAVARVVTPMLGHIRR
                     GVDHNRPAASRPSPQKSQARHAIR"
     gene            1479..2153
                     /locus_tag="PKK81_11005"
     CDS             1479..2153
                     /locus_tag="PKK81_11005"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNV33455.1"
                     /translation="MLKASLKRYFLTGLLIMIPFWGTILILKTLFVSLDGILGDATAQ
                     LVTPGYYVPGLGIVALILLIFVTGLFAANFIGRHVVRQWEGLLNRVPVVRGIYSTIKS
                     MMDILSFAERESYRRVVLIQFPKNGHYCFAFVTGVTKGEMQQLSPDPLVHVYVPTSPN
                     PTSGYFLLVPEREVIAVDITVEEAMKLIVSGGLYTPTPPSGGTSQTSGKTWAPIKQPD
                     AGVQVG"
     assembly_gap    2381..2480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2481..3632
                     /gene="glmU"
                     /locus_tag="PKK81_11010"
     CDS             <2481..3632
                     /gene="glmU"
                     /locus_tag="PKK81_11010"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNV33456.1"
                     /translation="SGYGRVIRRRRDEWLQGAADNAVQSIVEDKDASEAERAVREINV
                     GTYVVDGEFLFPALDKLDPRNAQGEYYLTDIVQMAVQQGRTVSALRLRAIDEGLGINS
                     RVQLAEAEQVIRQRIREQWLEAGVTMRDPASTWIDAEVTIGRDTVLYPNVTLEGRTVI
                     GESVVVHSGTRITDCAIGDRVEILDHCILRESQVEEECHLGPFVHLRPGVIARRKAKV
                     GNFVEMKKTELGEGSKANHLSYLGDATIGSGVNIGAGTITCNYDGYKKFHTVVGDGVF
                     IGSDVQLVAPVTVGQGSIIAAGATVTQDVPKDALVIARVPQVTREGWAARRRALQSGQ
                     VPPPAPSVKRAIPVKAAKAVKRAKTAKAATSATRAVKKKQPAVQRTKRR"
     gene            3642..>5380
                     /gene="glmS"
                     /locus_tag="PKK81_11015"
     CDS             3642..>5380
                     /gene="glmS"
                     /locus_tag="PKK81_11015"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNV33457.1"
                     /translation="MCGIVGYVGNQDAVPILLNGLSKLEYRGYDSAGVAIQRGEKIEI
                     RRSVGKLINLQKSLEQKAIGGMCGIGHTRWATHGKPSEQNAHPHRSESCVLVHNGIIE
                     NYVELKQRLVKDGYKFQSETDTEVVAHLIDTHMKKSKLHLADAVRATAKEIRGSYAIA
                     VISEREPGMLVAARSGCPLVIGRTADASFVGSDVMAMLSHTRDVTFLEEGDVVEVTAG
                     AVTFTDLDGRAVTRKKTKVTWDASAAEKSGYPHFMLKEIHEQPQTILDTIRGRYSYES
                     GEADLPDIGLTPKEFAEVGRIWIVACGTSWHAGLVGKYLLEEMVRTPVQVDIGSEFRY
                     RDPLIEKNDLFITISQSGETADTLAAAREAKQKGARVVSIVNVVGSTLARESDGVLYT
                     HCGPEIGVASTKAFTSQLAALYMLALHLGRVRGVLTVADGKAWLDRLVTLPALVKHVL
                     GREAEILAIAKRYYKKSDFLYLGRGINYPIALEGALKLKEISYIHAEGYAAGEMKHGP
                     IALIDKDMPVVVLAPRDRLYEKTVSNLMEVKARRAPVIAFVAEGERELGKIADAVFTI
                     PDVHPLLSPILFT"
BASE COUNT         1015 a         1444 c         1782 g         1039 t
ORIGIN      
        1 cattccaggg cccgccccaa ttggccgtcg ctgagcaccg ccaggaagtg cgcgtcggcg
       61 ggaggcagtt cgcgcttcag aatgacggcg gcttccacct gggtttgcgc gggtgccgtc
      121 aggcgcaggg cctggcagcg ggagcggatc gtagccggca gcgcataggg tcggctcgac
      181 accagaatga acaggctgtg gtcgggcggc tcttccaacg ttttcagcaa ggcattggcg
      241 gcgccgatcg tcatgcgatc cgcgtcgtcg atcaggcaga tcttccgatc accgatcagc
      301 gggcgataga tcatctggtg ttcgatgtcg cgaatgagct cgatcttgat ttggggattc
      361 gcgagttctt gatccggctc gatcaccatg aagtccggat gggtgcgcgc gtccacctgg
      421 cggcaggccc ggcaggcgcc gcaggcatcc ggagtcggat cgtccgggac ggtttcgcag
      481 aggagcgctt gcgccaggcg aagggcgagg agacgtttgc cgatgcgatc gtcgccatgg
      541 aagaggtagg cgtgcgcaag ccgattctgc aggattgcag accggagcaa ggttttggcg
      601 ctgtcatggc cgatgatatc agcgaatggc atggcgagcc tgtgacttct gaggtgacgg
      661 ccggcttgct gccggcctgt tgtggtctac gccccggcgg atgtgaccga gcatcggcgt
      721 gacgacccgg gcgactgcgc gggccaccgt ctctttcgat gcccttgcgg acaggacctt
      781 gattcgtgtg gggtgacgtt tggccagatc gagaaatccg gtccgaacgc tccggtgaaa
      841 ccggagagac tcgcgatcca gtcggttcaa ctcggcggcg gaacgccgtc gcgccagtcc
      901 cgtcgaaacg gggaggtcga acaccagcgt caggtccggc atgagcgcat gggtggcgag
      961 gcgattgagt cgttcgagcg tggcgatgtc tagtcctcgc gcatagccct gataggcaag
     1021 cgtggagtcg atgaaccggt cgcagagcac gatgctgcca cgcgcgagtg ccggttcgat
     1081 gacctgagcg acatgttgcc ggcgcgcggc gaggaccaga aacgcctcgg tttcgggcgc
     1141 caccggttcg tcggcgcgat ccaggagcag cgaacggatt ttttcagcga gcggagtgcc
     1201 gcccggctcc cgggtttcca ccgtgtcata tccctggttc cggaggtgct cgcccaggag
     1261 tttggcctgg gtcgattttc cgcacccctc ggttccctcg agggtaatga acaggccccg
     1321 gggtggtcgg ctacgtggcg tcatcattcg ggtctgaaaa actacggagc aagagggccg
     1381 gatcctattc caagtgattg aaaatagcaa gaaaaggctt gcgacgattg gtgaaagacc
     1441 agtatcatgc agctctacca agggcccttc agccacacat gttgaaagct tcactcaaac
     1501 gttattttct caccggtttg ctgatcatga tccccttctg ggggaccatc ctgattttga
     1561 aaaccctgtt cgtcagtctg gacggcattc tcggcgatgc tacggctcag ttggtgaccc
     1621 ccggctacta tgtgccgggc ttggggatcg tggcgctgat cctgctcatt tttgtcaccg
     1681 ggctgtttgc cgcgaatttt attggccgtc atgtggtgcg acagtgggag ggattgctca
     1741 atcgcgtgcc ggtcgtgcgc ggcatctatt ccactatcaa gtcgatgatg gacattctgt
     1801 cgtttgcgga gcgggagagt tatcgccgcg tcgtgctgat ccagtttccc aagaacggcc
     1861 actattgttt tgcgttcgtg accggggtga cgaaggggga aatgcagcag ctctcgccgg
     1921 atccgctggt gcacgtctac gtaccgacct cgcccaatcc cacgtccgga tatttcttgc
     1981 tggtgcccga gcgtgaagtg atcgccgtgg atattacggt ggaagaggcc atgaagctga
     2041 ttgtctcagg gggactctac acgccgactc ctccatcggg cggcacgtcg cagaccagcg
     2101 ggaagacgtg ggcgcctatt aagcaaccag acgcaggtgt gcaggtcggt tgatcgcgct
     2161 tgcactcgac ggacatcgcg tcgatttctt atggtgacga ctcaatgact cattcatcgc
     2221 aggacaaggc cgtgccatcc tccattcccg ggcttggtgt catcatcatg gcggcgggac
     2281 tcggcaagcg catgaagtcg gcgctcgcca aggtgctgca tccggtcgcg ggccggccga
     2341 tggtgatgta cgtactcgat atcgcctgcg gcctggcgga nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn tccggctacg gacgggtcat tcgccggcgt cgcgatgaat
     2521 ggctgcaagg cgcggcggac aacgcggtgc agagcattgt ggaagacaag gacgcctctg
     2581 aggccgaacg ggctgtgcgc gagatcaacg tcgggacgta cgtcgtcgac ggcgagtttc
     2641 tctttccggc gctcgacaaa ctcgatcccc ggaatgcgca aggcgagtat tacctcaccg
     2701 atattgtaca gatggcggtc cagcagggac gcacggtgtc ggctctgcgt ctccgcgcga
     2761 tcgacgaggg actcgggatc aatagccgtg tgcaattggc ggaggctgag caggtgattc
     2821 gccagcggat tcgggagcaa tggctggaag ccggtgtcac gatgcgggat cctgcctcga
     2881 cctggatcga tgctgaggtg accatcggcc gtgacacggt gctgtatccg aatgtgacgc
     2941 tcgaaggccg gaccgtgatc ggcgaatccg ttgtcgtgca ttccggcacc cggattactg
     3001 attgcgccat cggcgacagg gtagagattc tggatcactg cattctgcgc gagtcgcagg
     3061 tggaggagga gtgccatctc ggaccgttcg tgcacctgcg accgggtgtg atcgcgcgac
     3121 gcaaggcgaa ggtcggcaat ttcgtcgaga tgaaaaagac ggaactcggt gagggctcta
     3181 aggcgaacca tttgagttat cttggagacg ccacgatcgg gtcgggagtg aatatcggcg
     3241 ccggaacgat cacctgcaat tacgacggct ataagaaatt tcacacggtg gtaggggacg
     3301 gtgtgttcat cggcagcgac gtgcagctcg ttgcgccggt gacggtcggg caaggttcca
     3361 tcatcgcggc cggcgccacc gtgacgcagg atgtgccgaa ggatgcattg gtgattgccc
     3421 gggttcccca ggtgacccgc gagggttggg cggccaggcg gcgcgcgtta cagagcgggc
     3481 aggtcccgcc tccggctccc tcggtgaagc gcgcaatccc tgtgaaggcc gcaaaggctg
     3541 tgaagcgtgc gaagactgca aaggcagcaa catccgcaac gcgtgccgtg aagaagaagc
     3601 aaccggcagt tcaacgtaca aaacggaggt aacggcgacc tatgtgtggc atcgttggct
     3661 acgtgggaaa tcaggacgca gtaccaattt tgttgaacgg gttgtcgaag ctggaatatc
     3721 gcgggtacga ctcggccggc gtggcgattc agcgcgggga gaagatcgag attcgccgca
     3781 gcgtcggcaa gctgatcaat ctgcaaaagt cgctggagca gaaggccatc ggcgggatgt
     3841 gcggcatcgg gcacacgcgt tgggcgacgc acggcaagcc ctccgagcag aacgcccacc
     3901 cgcaccgctc cgagagttgt gtcctggtgc acaacggaat catcgaaaat tacgtcgagc
     3961 tgaagcagcg cctggtcaag gacggctaca agtttcagtc tgaaaccgac acggaagtcg
     4021 tggcgcacct gatcgataca cacatgaaga aaagtaagct gcacctggcg gatgcggtgc
     4081 gcgcgacggc gaaagagatt cgcggaagtt atgccatcgc ggtgatttcg gagcgggagc
     4141 ccggcatgct ggtcgcggcc cggtccggtt gtccgctggt catcggtcgc acggccgatg
     4201 cgtcgttcgt cggctcggac gtgatggcga tgttgtccca taccagagac gtgacgtttc
     4261 tcgaagaagg cgacgtggtc gaagtcacgg ccggagcggt gacgtttacg gatctcgacg
     4321 gtcgggccgt cacgcgcaag aagacgaagg tcacgtggga tgcctctgcc gctgaaaaga
     4381 gcgggtaccc ccacttcatg ctgaaggaaa ttcacgaaca gccgcagacg attctggata
     4441 ccatccgggg ccgctattcc tatgagagcg gcgaggcgga tcttccggat atcggattga
     4501 cgccgaaaga gttcgccgaa gtcgggcgta tttggatcgt ggcctgcggc accagttggc
     4561 atgccggcct cgtggggaag tatctgctcg aagagatggt tcgcacgccg gtgcaggtgg
     4621 atatcgggag cgagttccgg tatcgcgacc cgctcattga aaagaacgac ctgttcatca
     4681 cgatctcgca gtcgggtgaa accgccgata cgctggccgc ggcgcgggaa gcgaagcaaa
     4741 aaggggcgcg tgtcgtttcg atcgtcaatg tggtgggcag caccctggcc cgcgagtccg
     4801 acggggtgct ctacacccat tgcggtcctg aaatcggcgt cgcctccacg aaggccttca
     4861 ccagccagtt ggcggcgctc tacatgctgg ccttgcatct gggccgggtg cggggtgtcc
     4921 tgactgtggc ggacgggaag gcctggctcg atcgcctggt cacgttgccg gcattggtga
     4981 aacatgtgct tggccgggaa gcggagattc tggcgatcgc gaaacgctat tacaagaaat
     5041 cggatttttt gtatctgggg cgcgggatca actatccgat cgcgctggag ggggcgctca
     5101 agctcaagga gatttcgtat atccatgcgg agggttatgc cgcgggagag atgaagcacg
     5161 gaccgatcgc gctcatcgac aaggacatgc cggtggtggt gttggcccct cgggaccggc
     5221 tctatgagaa gaccgtcagt aacctcatgg aggtgaaagc gcgccgggcc cccgtgattg
     5281 cgtttgtggc ggaaggcgag cgcgagttgg gcaagatcgc cgacgcggtc ttcaccattc
     5341 ccgatgtgca tccgttgctg tcgcccattc tctttacgat
//
LOCUS       DAOQRM010000159         5359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_15799_length_5359_cov_17.076357, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000159 DAOQRM010000000
VERSION     DAOQRM010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15799_length_5359_cov_17.076357"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..682
                     /locus_tag="PKK81_11020"
     CDS             98..682
                     /locus_tag="PKK81_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNV33458.1"
                     /translation="MMVLPQTCERALLERFLRAEAMALWAVRAAQTQNLPRHVQTFLQ
                     RHETDEQDHLRQFEGMLGTTSQRAPTLPTVPSQWEMLAVLLFGYEALGLEFATLLATV
                     RPDLADILEDEQVHVGFFEKELRAILAGGESGAQQAREAARTWWKKLPRTVDRYLGDP
                     SLAPYRTELRHHILSVIQERFIALGLLPAGQAGR"
     gene            complement(715..>1797)
                     /locus_tag="PKK81_11025"
     CDS             complement(715..>1797)
                     /locus_tag="PKK81_11025"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNV33459.1"
                     /translation="DGFLRVRGWVDGGYTYNASNPRSNFSGPYNAIDRDIPVLNQLYM
                     IVEKPLTATGSNWGIGGRLDFMYGYDYFLTQSNGVERRENGAQGWNAQGQHYGLAMPQ
                     AYAEIGNQTFSVKVGHFYTIIGYEGVPSINNFFYSKSFAYQFAGPFTHWGGLATWHLN
                     DRITLQGGLVNGWDSLDRTKDSTTGLASIKYTAPENLWSLSFSMVTGNEPSAVATQFE
                     TRTRYSLIFTAHPAERWEYVFHHHYAYQNQGKVDGGTARWYGIDNYLYYALTDQWRAG
                     LRFEWMRDEAGTRVGGNPVRDNPNLGPFAGSFYSLSAGLNYRPHPNVLIRPEVRADWF
                     DGQRSPYNDGLNKNQVLAAINGTLQF"
     assembly_gap    1798..1897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2084..3052)
                     /locus_tag="PKK81_11030"
     CDS             complement(2084..3052)
                     /locus_tag="PKK81_11030"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNV33460.1"
                     /translation="MRDYLDFEKPIRELEEKIEKLASAESPKSGTQDEIRKLRTKLAQ
                     VEHQLYTTLTPWQRTQLARHPQRPTTLDYINELSREFLELHGDRSFGDDRAIVGGFAR
                     FNDRSVMIIGHQKGKTLKERMQRNFGMPNPEGYRKALRLMRLAEKFGRPIITLIDTPG
                     AYPGIGAEERGQAEAIARNLFVMSRLSVPIISVVIGEGGSGGALALGVSDRILMLEHS
                     VYSVISPEGCAAILYDDPSKVPDAAASLKMTAQDLVGLGIVDEVIPEPLGGAHRDPRA
                     MCDRVAKTLANQLYALVELPTDQLIAQRDQKFRKIGVVGGLVPVGA"
     gene            complement(3090..>5359)
                     /gene="dnaE"
                     /locus_tag="PKK81_11035"
     CDS             complement(3090..>5359)
                     /gene="dnaE"
                     /locus_tag="PKK81_11035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNV33461.1"
                     /translation="NPERVSLPDIDMDFCMDRRDEVINYVIHKYGEDHVAQIITFGTM
                     KAKAAIRDVGRVLEMPYAEVDRIAKLVPDDLKMTLDKALEQEPRLKELVDKDVKVKEL
                     MSVAQSLEGLARHASTHAAGIVISDQPLTEHVPLYKGPKDEIVTQYSMGDVEKIGLVK
                     FDFLGLKTLTMIHRAETLVNEVRPGQPPLLVEQLAFDDPETFALLSSGKTTGVFQLES
                     SGMRDLLMGLKPDRFEDIIAIIALYRPGPMDLIPDFIKRKQGKIPIAYEMPELEPILK
                     DTYGVIVYQEQVMAIANKVAGFSLGQADILRRAMGKKKPEEMEKLRVQFLEGAKQKKI
                     SEKKADKLYELIQKFAGYGFNKSHAAAYAVVCYQTAYLKAHYPTEFMAALMTTDMGNA
                     DKIVGYFTECRGLGIPVLPPDVNQSQKNFAVVDKSIRFGLAAIKNVGEGAVEAIIEVR
                     NETGPFRSFFDLFRRVDLRKLNKRMMEGLIKAGAFDSMGGRRSQYLAVLDQAMDEGMS
                     IQKERALGQTSIFGDDTIGMPQQLDEPALPDVPEWSQGELLKYERELTGFYISAHPLA
                     RYEAAIQLFANTTTMQIPDVPDGREVKLCGIITAVKSMLTKKGDRMAYITLEDLHGLV
                     EVIAFPDLYRDHADMIVPERVVRLTGTVDRGDKGTKLRGTKIEPLADLQNTGISRVMI
                     HLHDGPTASTRLPMLRQVFQKYPGASSVSLVMALGGTMEARTSPLPNVKITPSEHFVA
                     DIEEVLGKGAITLVT"
BASE COUNT          934 a         1488 c         1687 g         1150 t
ORIGIN      
        1 tatttccaat ggcccgatgg agcctcgcag gaagtcggac tgacgaaggg tgagtgggcc
       61 ggtccgatcg ccccgccgag tgaggattga cggtgcgatg atggtcctgc ctcagacctg
      121 tgaacgggcg ttgctcgaac ggttcttgcg cgccgaagcc atggcgctct gggcggtgcg
      181 ggccgctcag acgcagaatc ttccccgcca cgtccagacg tttctccagc gccacgagac
      241 cgacgagcag gatcacctgc ggcaattcga agggatgctg ggtaccacgt cgcagcgggc
      301 gccgactctg ccgactgtgc cgtcgcagtg ggaaatgctg gcggtgttgt tgttcgggta
      361 tgaggcgctc ggactggaat tcgcgacgct cctcgcgacg gtgcggccgg acctggcgga
      421 catcctcgaa gacgaacagg tgcatgtcgg attcttcgag aaggaattga gggcgattct
      481 ggcgggcggg gagagcggag ctcaacaggc gcgggaggcg gcacgcacct ggtggaagaa
      541 gctgcctcgc acggtggacc ggtatctcgg tgatccctct ctcgcgccgt accggacgga
      601 acttcgacac cacatcctgt cggtcattca ggagcgattc atcgcgttgg gtctcctgcc
      661 tgccgggcag gcaggccggt aatctccgac ccgcctgctc ggttccacct cgcgctagaa
      721 ctgcagggtg ccgttgatgg cggctaagac ctgattcttg ttcaggccgt cgttataggg
      781 cgagcgctgc ccgtcgaacc aatcggcgcg cacttccgga cggatcaaca cattgggatg
      841 cggccggtag ttgaggccgg cgctgagcga atagaaggat ccggcgaacg gcccgaggtt
      901 cgggttgtcg cgcaccgggt tgccgccgac cctggtgccg gcttcgtcgc gcatccactc
      961 gaagcgcaga ccggcccgcc attgatcggt gagcgcatag tacagatagt tgtcgatgcc
     1021 gtaccagcgc gcggtgccgc cgtcgacctt gccctggttt tgataggcat agtgatggtg
     1081 gaagacgtat tcccatcgct cggccggatg tgccgtgaag atcaggctgt atcgcgtgcg
     1141 ggtttcaaat tgtgtggcca cggcgctggg ttcgttgccc gtgaccatgg agaatgagag
     1201 cgaccagaga ttctccggcg cggtgtactt gatgctggcc agacccgtgg tgctgtcctt
     1261 cgtccggtcc agcgaatccc agccgttcac caggccgcct tgcaacgtga tgcggtcgtt
     1321 caggtgccag gtggccagtc cgccccagtg ggtgaagggg ccggcgaatt ggtaggcaaa
     1381 ggatttggag tagaagaagt tgttgatgga cggcacacct tcgtagccga tgatcgtata
     1441 gaagtggccg accttcaccg agaaggtctg gttcccgatt tcggcatagg cctgcggcat
     1501 cgccagcccg tagtgctgac cctgggcgtt ccagccctgc gcgccgttct cacgccgctc
     1561 gactccgttg ctctgtgtca ggaagtagtc gtagccatac atgaaatcca agcgtccgcc
     1621 gatgccccaa ttgctgcccg tggcggtcag cggtttctcg acaatcatgt acaactggtt
     1681 gaggaccgga atgtcgcggt cgatggcgtt gtagggtccg ctgaagttgc tgcgcgggtt
     1741 gcttgcatta tacgtatacc cgccgtccac ccagccgcgc acgcggagga atccgtcnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttc ttgtcgagac gggacccatt
     1921 cagcgcacgc aggcgggcgg cagtgtaaca agtcgtcggg caatgaaaaa tcaggacgag
     1981 tatgtaggtg gtttcagcgg gtcttgatgg ccagggccgg atgcagggga tagagcaacg
     2041 ttaagtcagg cgtgcaatgg tggagaggaa gaattccagg gggttaggcc cctaccggta
     2101 cgagcccgcc gaccaccccg atcttgcgga atttctgatc gcgctgggcg atgagctggt
     2161 cggtcggcag ttcaaccagg gcatacaact gattggccaa ggtcttggcg acccgatcgc
     2221 acatggcccg ggggtcacga tgcgcgccgc ccaacggttc cggaatcact tcatcgacga
     2281 tgccgagtcc gaccagatcc tgcgcggtca ttttcagcga ggctgcggca tccggaacct
     2341 ttgacgggtc atcgtacaga atcgccgcgc agccttcagg ggaaatgacg gagtagacgg
     2401 aatgttccag catgaggatg cggtcgctca cgcccaacgc cagggcgccg ccgctgccgc
     2461 cttcgccgat gacgacggat atgatgggca ctgacagtcg ggacatgacg aacaggtttc
     2521 tggcgatggc ttcggcctgt ccgcgttctt ccgcgccgat accgggatag gcgccggggg
     2581 tgtcgatcag cgtaatgatc ggacggccga acttttccgc cagccgcatg agccgcaatg
     2641 ccttccggta gccttccgga ttgggcatgc cgaagttccg ctgcatacgc tctttgagcg
     2701 tctttccttt ttggtgcccg ataatcatga ctgagcgatc gttgaagcgg gcgaagccgc
     2761 ccacgattgc gcggtcgtcg ccgaagctgc ggtcgccgtg cagttcgagg aattcgcgag
     2821 agagttcgtt gatatagtcg agggtcgtgg gccgctgagg atggcgggcc agttgggtgc
     2881 gctgccaggg cgtcagggtt gtatagagct ggtgctcgac ctgggcgagt tttgtccgga
     2941 gcttgcggat ttcgtcctgc gtgccggact tgggagactc ggcggaggcc agcttctcga
     3001 ttttttcttc gagttcgcgg atcggttttt caaagtcgag gtagtctctc atactgctga
     3061 acccccttaa gggtgttgcc taggttcgcc taggttacca aagtgatggc gcccttgcct
     3121 agcacttcct caatatcggc gacaaaatgc tcgctgggag tgatcttgac gttggggagg
     3181 ggagaggttc gcgcttccat tgtaccgccg agggccatga ccagagagac gctcgaagca
     3241 ccgggatatt tctggaacac ctgtcggagc atcggcagtc tggtggaggc cgtcggtccg
     3301 tcgtgcaggt gaatcatcac ccgcgaaatc cctgtgttct gcaaatcagc cagcggttcg
     3361 atcttggtcc cgcgcaactt ggtgcccttg tctccgcgat ccacggtgcc cgtcaatcgc
     3421 acgacccgtt ccggcacgat catgtcggca tggtctcgat acaggtcggg gaaggctatc
     3481 acttctacca gcccatgtaa atcctctagt gtgatgtagg ccatgcggtc gccttttttg
     3541 gtgagcatgg atttcaccgc cgtgatgatc ccgcagagtt tgacctcgcg tccgtccggc
     3601 acatcgggga tctgcatggt cgtggtgttg gcgaagagtt ggatggccgc ttcataccgg
     3661 gccagcggat gggcgctgat gtagaagcca gtcaactccc gctcgtattt cagcagctcg
     3721 ccctggctcc attctggcac gtcgggcaag gccggctcat ccagttgctg cggcataccg
     3781 atggtgtcgt cgccaaagat gctggtctgg ccgagcgcgc gctctttctg gatgctcatc
     3841 ccttcgtcca tggcctggtc gagcacggcc aggtattgcg agcgccgtcc gcccatggaa
     3901 tcgaaggccc cggctttgat caacccttcc atcatgcgtt tgttcaactt gcggagatcg
     3961 acccggcgga agaggtcgaa gaaggagcga aacggtccgg tctcgttgcg cacttcaatg
     4021 atggcttcaa ccgcgccttc gccgacattc ttgatcgccg cgagcccgaa gcggatgctc
     4081 ttgtccacga cggcgaaatt cttctggctc tggttcacgt ccggtggcag caccggaatg
     4141 ccgagcccgc gacattcggt gaagtagccg acgatcttgt cggcgttgcc catgtcggtg
     4201 gtcatcagcg ccgccatgaa ctcggtgggg taatgcgcct tgagatacgc cgtctggtaa
     4261 cagaccaccg cataggcggc ggcgtgcgat ttgttgaagc cgtatccggc aaacttctgg
     4321 atgagttcgt agagtttgtc ggccttcttc tcggatattt ttttctgttt cgcgccttcc
     4381 aggaattgga cgcggagctt ctccatctcc tcgggcttct tcttgcccat ggcgcgacgg
     4441 agaatatccg cttgtcccag cgagaatccg gccaccttgt tggcgatggc catgacctgt
     4501 tcctggtaca cgatgacgcc gtacgtgtcc ttcaggatcg gctcgagctc gggcatctca
     4561 taggcgatcg ggatcttgcc ctgtttgcgc ttgatgaagt ccggaatgag gtccatcggg
     4621 ccggggcgat agagcgcgat gatggcgatg atgtcttcga accggtccgg tttcaggccc
     4681 attaagaggt cgcgcatgcc ggaactttcg agctggaaga cgccggtggt ttttccggac
     4741 gacagcagcg caaatgtttc gggatcgtcg aacgccagtt gctcgacgag caagggcggc
     4801 tgaccggggc gcacttcgtt caccaacgtc tctgcgcggt gaatcatggt gagcgttttc
     4861 agtccaagga agtcgaactt caccaggccg attttttcga catcacccat cgaatattgg
     4921 gtgacgattt cgtccttggg gcccttgtaa agcgggacgt gttcggtgag cggctgatcg
     4981 gagatcacga taccggccgc gtgtgtggag gcatggcggg cgaggccttc gagcgactgc
     5041 gccaccgaca tgagttcctt caccttcaca tctttatcga cgagttcctt caggcgcggc
     5101 tcctgttcga gcgccttgtc gagggtcatc tttaaatcat ccgggacgag cttcgcgatt
     5161 cgatccactt cggcgtaggg catttcgagc acgcggccca cgtctcgaat ggcggccttg
     5221 gccttcatcg tcccgaaggt gatgatctgc gcgacatggt cttcgccgta tttatggatg
     5281 acgtagttga tgacttcatc ccggcgatcc atgcagaagt ccatgtcgat gtcggggagg
     5341 gacacacgct caggattca
//
LOCUS       DAOQRM010000160         5293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16114_length_5293_cov_13.352997, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000160 DAOQRM010000000
VERSION     DAOQRM010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16114_length_5293_cov_13.352997"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1225)
                     /locus_tag="PKK81_11040"
     CDS             complement(110..1225)
                     /locus_tag="PKK81_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNV33462.1"
                     /translation="MDTTTQPTTTQPSAIEKVFAFVDERVGLKTLQAKMLNEPVPGGS
                     RWAYVFGSVLLFIFIMQAVTGILLMFYYVPTADHAYASTQYIIHSVDYGWFLLSYHFW
                     GSTAMVVCVFAHMSQVFLWGAYKKPRELIWLVGLALFGIVMGFGFTGYLLPWDQRAYW
                     ATTVGVEIMDKTPLLGDFMARFLKGGATPGQMTLSRFFVIHVMVLPAALMGLAGLHLF
                     LFRKAGPAGPFRGSVEEIKAKTDYFFPRQIWKDIVGMVAVFLCICSLAFWEPIVLLEE
                     ATPDPGDYHPEPEWYFLFLFQLLRLKVFAGEFGQFLGAIALPGAFMALLAALPFIDRS
                     PERNIFKRPIALIGWIVVMASILIFTVAAIINREFLE"
     gene            complement(1226..2164)
                     /locus_tag="PKK81_11045"
     CDS             complement(1226..2164)
                     /locus_tag="PKK81_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNV33463.1"
                     /translation="MQPKLKSKVRCTDGEVGEVRRVIMDPLSHEISHIVVGSGAVDSV
                     ERQVPMGQVQGVAEEVVTLRGTSAEYATLPVFKRDDYVTTHEVEIAHLEERIHVTPGE
                     VLVPFPELERSVKRRTFFANFTHAIGFLIGLPIAFPVLRYLMKPMYSPFDNQWLKIGN
                     SGKIKQDDVGVQFKFKRKIKEAYMPEQEIDKNVWVLKATPKVLETIYQGKDMEFHDAS
                     GKVIWTNKKDVPYIAYSGKCPHLGCGFKWRTHKTLGQVFLCPCHLSIYDAAGKVLDGP
                     APRALDPLPIKVTAAGDITIIDMEFKAGTKAQVRIV"
     gene            complement(2170..2976)
                     /locus_tag="PKK81_11050"
     CDS             complement(2170..2976)
                     /locus_tag="PKK81_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV33464.1"
                     /translation="MGNVIKKSLVGIVIGGILFGATNALGFPFVFQVLFFGYAMLGAV
                     VFIILDLPPLKPISGVKAAGALVVFYAILSVAYIAGGAVWPQFDPEDEKGKIDKILKP
                     KREHYEAGKVEVLLQRAKALDEKAKELTARLQALGAAQAPADQKAGGETAPTATTAAA
                     GGDIAKLGEEQWQLQECYNCHKLNGEGGKKRGPELDNIGNLLSAEEIATKILDPKSYK
                     AEGFEKEYEKGKMPDKYKDLMEPKDVQALAGWLASYKNASVNTPKPIKMK"
     gene            complement(3016..4719)
                     /locus_tag="PKK81_11055"
     CDS             complement(3016..4719)
                     /locus_tag="PKK81_11055"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNV33465.1"
                     /translation="MKIWQRCLLVMFALVAILGGVAYAQAPSAPPVEFPYTGNRTAVW
                     IVAQLHILFAGFILGAPIFVVISEWLGYRKQDPRYDRLAKEVTKVTVILYSMTALTGG
                     LFIFVLLATYPQFTTWLINHFFLIFAVVYPLLFIGETIILYMYFYTWDAWKGEKKARH
                     IALGVLLNLIGSITLFVIDGPTSFMNTPVRAEGVSPAEFLATASLWDKVFNYSWMPLN
                     LHRLVGNVTFGGFIAGLIAAYMFMGSKKEEERAYYDWMGFVGNMIGVGALLFLPFMGY
                     LLSYELCDYDASICPYMMADQLSMFFEMQGAMVGLIFLASNYYIWLSMKRIEGVERVR
                     MTVIAPIVMVLLPIVMTKVLTDYPVPDATSLAFLVPLLLAPVILGRFIPLTVSSSTVI
                     KVGFLMVVVGNAIWMTPHGFVPTGAKLVAELELPSDWNFLALMPAKNSAAFTLIFVTV
                     VNYVIYNRAVSQGTIVWGKIDFASQFVLVFLAFSAIWTMGLMGAVRSLLRKYFHAYNL
                     LPDFTAESFTPTLSYSAWWITGITVTFFAVVSFAIIVTLRPSDSKGHAHEGSPVPAGA
                     K"
     gene            complement(4785..>5293)
                     /locus_tag="PKK81_11060"
     CDS             complement(4785..>5293)
                     /locus_tag="PKK81_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV33466.1"
                     /translation="FMKQAGMKRLALMVGILGFGFGVAACGGGEGGGEGPVVPPPPAP
                     AEYADKHMPAGWWTDPKIIEEGKELYIGGKNPDVNCASCHGKDGKPVKAGARDFRNGD
                     RMKLYSDSVWFWRISEGVPNTKMKPWKSKLSEEDRWKILAFERSFGLAGKGWDANKKD
                     WVPADQVK"
BASE COUNT         1268 a         1587 c         1367 g         1071 t
ORIGIN      
        1 acgccatctt gatcggcagg gcggtccagc aggccggcca gagaatgcta agccctaatt
       61 taaatgacga catcgtctct cgaccagtca tgtgcccaaa cccctccgcc tattcgagaa
      121 attcgcggtt gatgattgcc gccacggtga aaatcagaat gctcgccatg accacaatcc
      181 atccgatcaa ggcgatcggt cgcttgaaga tattgcgttc cgggctgcga tcgatgaacg
      241 gcaatgccgc gagcaacgcc atgaacgcgc ccggcaaggc aatggcgccg aggaactgcc
      301 cgaactctcc ggcaaacacc ttcagacgca gcagttggaa caagaagagg aagtaccatt
      361 ccggttccgg gtggtaatct ccgggatccg gcgtggcctc ttcgaggagc acaatcggct
      421 cccagaacgc gaggctacag atacacagga agaccgcgac catcccgaca atgtctttcc
      481 agatttgacg gggaaagaaa tagtcggtct tggccttaat ttcttccacg ctgccccgga
      541 agggtccggc agggccagcc ttacggaaca ggaacagatg cagtcccgcg agccccatca
      601 aggccgccgg aagaaccatg acgtgaatga cgaagaagcg actcagcgtc atctggcctg
      661 gcgtcgcgcc gcccttcagg aagcgcgcca tgaagtcgcc gagaagcggc gtcttgtcca
      721 tgatctcaac accgaccgtc gtggcccagt aggcccgttg atcccacggc agtagatagc
      781 cggtaaagcc gaatcccatg acaatgccga agagggccaa accgaccaac cagatgagtt
      841 cgcgcggttt cttgtaggcg ccccagagaa agacttggga catatgggcg aacacgcaca
      901 cgaccatcgc cgtggatccc caaaagtgat agctcaacaa gaaccagccg taatccaccg
      961 agtggatgat gtattgggtg ctcgcatagg catggtcggc cgtcggcacg taatagaaca
     1021 tcaacaaaat gccagtaacc gcctgcatga tgaaaataaa gagcaggacg gagccaaata
     1081 catacgccca tcgcgacccg cctgggacgg gctcgttgag cattttggct tgcagagtct
     1141 tcagcccgac gcgttcgtcg acaaaggcga agactttttc aatggctgag ggctgagtgg
     1201 tcgtcggctg cgtagtcgta tccatttaga caatccgaac ctgggccttg gtgcccgcct
     1261 tgaattccat atcgataatg gtgatgtcgc ccgctgcagt aaccttaatc gggagcggat
     1321 ccaacgcacg cggagccggc ccatcaagga cctttccggc tgcgtcatag atgctcaagt
     1381 gacaagggca cagaaagacc tggccgagcg tcttatgcgt tcgccacttg aaaccgcacc
     1441 ccaggtgagg acacttgccg gaatacgcga tgtagggcac gtctttctta ttggtccaga
     1501 tgaccttccc ggatgcgtca tgaaactcca tgtctttacc ctgataaatc gtctccagca
     1561 cctttggagt cgccttcaag acccagacgt ttttgtcgat ttcctgctcc ggcatgtagg
     1621 cttctttaat ttttcttttg aacttgaact gcacgccgac gtcatcctgc ttgatctttc
     1681 cggagttgcc gatcttgagc cattgattgt cgaaggggga atacatcggc ttcatcagat
     1741 agcgcaagac aggaaacgca atcgggagac cgatgagaaa tccgatggca tgggtaaagt
     1801 tcgcaaaaaa ggtccgacgt ttgacgcttc tttcaagctc aggaaaggga accagcactt
     1861 cccctggcgt cacgtgaatg cgctcttcaa gatgggcgat ttccacctcg tgagtcgtca
     1921 cgtagtcgtc acgcttgaag accggaagtg tcgcgtactc tgccgaggtg ccacgaagcg
     1981 tcacgacctc ttcggcgact ccttgcacct gccccatggg gacctggcgc tcgaccgaat
     2041 ccaccgcgcc ggaaccgacc acgatatggc taatctcgtg ggagagcggg tccataatga
     2101 ccctgcgcac ctccccgact tcaccgtccg tacatcgaac tttggatttc agtttcggct
     2161 gcatgatact cacttcatct tgatgggctt cggagtattg accgaagcat tcttgtaact
     2221 ggccaaccaa cccgccagcg cctgcacatc cttaggctcc atcaggtcct tgtacttgtc
     2281 gggcatcttc cctttttcgt actccttctc gaagccttcg gccttataac tcttcgggtc
     2341 gagaatcttt gtcgcgattt cctcagccga caggaggttg ccaatgttgt ctagttccgg
     2401 gccacgcttc ttaccgcctt cgccgttcag cttgtggcag ttgtagcact cctgaagttg
     2461 ccattgctcc tcaccgagct tggcgatatc accacccgca gcggctgtcg tcgccgtcgg
     2521 tgcagtctct ccaccggcct tttgatccgc cggagcctgc gcagcaccca acgcctgcaa
     2581 acgcgccgtc agttctttgg ccttttcatc gagcgccttg gcgcgctgca acagaacctc
     2641 gactttgccg gcctcgtaat gttctcgctt cggcttcaag atcttgtcga tcttgccttt
     2701 ttcgtcttcc gggtcgaact gcggccacac agcgccacct gcaatatagg cgactgatag
     2761 aatggcgtag aacacgacga gggctcccgc agcttttacc ccgctgatcg gcttgagcgg
     2821 agggaggtcg agaataataa agacgacggc gccgagcatg gcatagccaa aaaacagcac
     2881 ctggaacaca aacggaaatc ccagtgcgtt ggtggctccg aatagaattc cgccgatcac
     2941 gattccaact agcgacttct taatcacgtt tcccatgagt accctcgttt atcgcccttc
     3001 gagtattgtg agcgcttact tagcaccggc cggcacaggg ctcccctcgt gcgcatggcc
     3061 cttcgaatcc gaggggcgca atgtcacgat gatggcaaag ctgaccacgg caaaaaaggt
     3121 cacggtaata cctgtaatcc accaggccga gtaagacagc gttggcgtga aggactcggc
     3181 ggtaaaatca ggcagcaggt tgtaggcgtg aaaatacttc cgcaacagcg atcgtacggc
     3241 acccatcagc cccatggtcc aaatggcgct gaaggccagg aagaccaaaa cgaactgtga
     3301 ggcgaaatcg atcttacccc acacaatggt cccctgcgaa accgcacggt tgtagatgac
     3361 atagttcacc accgtcacga agatcaaggt gaacgcggct gagtttttcg ccggcatcag
     3421 tgcaaggaag ttccagtcgg atggcaattc caactccgca accagcttgg ccccagtggg
     3481 cacaaaaccg tgcggcgtca tccagatcgc attccccacg accaccatca agaagccgac
     3541 cttgatgacc gtgctggacg atactgtcag aggtataaat cgccccagaa taacgggcgc
     3601 caacaggagc ggcacgagga acgccagcga tgtcgcatca ggcactggat agtcggtcag
     3661 gaccttggtc atgacgatcg gcagcaacac catcacgatc ggcgcgatca cggtcatacg
     3721 gacccgctcc accccctcga tccgcttcat gctcaaccag atatagtaat tgctggccaa
     3781 gaaaatcagg cccaccatgg cgccctgcat ttcgaagaac atcgagagct ggtcggccat
     3841 catgtacgga caaattgagg cgtcgtagtc gcagagctca taggacagca aataacccat
     3901 aaacggcaag aacaagagcg cgccgacacc gatcatgttg ccgacaaacc ccatccaatc
     3961 gtagtaggcc cgctcttctt ccttcttcga gcccatgaac atgtaggcgg caatcaggcc
     4021 ggcgatgaat ccaccgaacg tcacgttacc gacaagccta tggagattaa gcggcatcca
     4081 gctatagttg aacaccttat cccacaggga agcggtcgcc aggaattccg ccggcgagac
     4141 gccctcggcc ctcacagggg tattcatgaa ggaggtcgga ccgtcgatca cgaacagcgt
     4201 gatcgaaccg atgagattca acagcacgcc cagcgcgata tggcgcgcct tcttctcacc
     4261 cttccacgca tcccacgtat aaaaatacat gtagaggatg atggtctcgc cgataaacaa
     4321 cagcggatac accaccgcaa atatcaggaa gaagtgattg atgagccagg tcgtgaactg
     4381 tggataggtt gcgagcaaca cgaagataaa gagaccgccc gttaatgccg tcatgctgta
     4441 gagaatgacc gtgaccttgg tgacctcttt cgccagccgg tcatagcgtg gatcctgctt
     4501 gcgatacccc aaccattccg agatgacgac gaaaatcggg gcccccagaa tgaatcccgc
     4561 aaagagaatg tggagctgag cgacgatcca taccgccgtg cgattcccgg tataagggaa
     4621 ctccacgggg ggcgcactgg gagcctgggc gtatgccaca cctcccaata tcgccactag
     4681 cgcgaacata accaacaaac agcgctgcca gattttcatg atgtctcgcc gctcctcctc
     4741 attccaaccg attgacccat gactcgaacc gaacccggcg tgagttactt cacctgatca
     4801 gcaggtaccc aatccttctt gttggcatcc cagcccttac ctgccaaacc gaaggaacgt
     4861 tcaaacgcca aaatcttcca acgatcctct tcggacaact tgctcttcca gggcttcatc
     4921 ttggtgttgg gaacgccctc agaaatacgc cagaaccaga ccgagtcaga atacaacttc
     4981 atgcgatcgc cattgcggaa gtcccgggcg cccgccttga ccggcttgcc gtccttacca
     5041 tggcaactgg cgcaattgac gtcggggttc ttgccgccga tgtacaactc cttaccttct
     5101 tcgataattt tcggatccgt ccaccaaccg gcaggcatat gcttgtcggc atactccgcg
     5161 ggagcgggtg gcggcggaac tacggggcct tcgcctcccc cttcaccacc gccgcaggcg
     5221 gcgacgccaa aaccgaaccc aagaatgccg accatcagag ccaatctctt catgcctgct
     5281 tgtttcataa aaa
//
LOCUS       DAOQRM010000161         5291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16125_length_5291_cov_11.463522, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000161 DAOQRM010000000
VERSION     DAOQRM010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16125_length_5291_cov_11.463522"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..1172)
                     /locus_tag="PKK81_11065"
     CDS             complement(309..1172)
                     /locus_tag="PKK81_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV33467.1"
                     /translation="MKVLLAVDGSDHSYEAVRALKYLRRPDELTLLHVVDAPRPAYPM
                     MVPEVAQELYSQLERSMKEDGEQLLTRIHSLLPPHSGPVTKQLASGSPAEMIVATAES
                     RHVDLILMGARGLGPVKERLLGSVSHRILSLAPCAKLILQGSLRDLKQVLLPLEGQSD
                     AEAALRFLRKQPFHEPVNINLLAVLPPTRPPWPVDDSAAEKLETQALRHARDFVDGVA
                     TELRTLGYTTRSASVLGTPVTMILHEAEKMGVDLVMVGSRARQGITRFVLGSVSHAVL
                     HRAPCQVLVFE"
     gene            complement(1169..1843)
                     /locus_tag="PKK81_11070"
     CDS             complement(1169..1843)
                     /locus_tag="PKK81_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNV33468.1"
                     /translation="MEAESIFQVRISHDGITLDGILGLPAHPRGVIAFAHGSGSGRFS
                     PRNQFVARHLETGGFATLLMDLLTPDEADDRRKVFDIDLLADRLLLAERWLQAHRQTA
                     GLRVGYFGASTGAGAALQAAAREPQAVGAIVSRGGRPDLAGPYLSRVTAPTLLLVGGD
                     DGPVIEMNQDALRQLTCRKELVIVPGASHLFEEPGTLEQVAREALRWFQRYLQPDAHA
                     AKESRS"
     gene            complement(1908..2003)
                     /locus_tag="PKK81_11075"
     CDS             complement(1908..2003)
                     /locus_tag="PKK81_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HNV33469.1"
                     /translation="MSRTQWLLFGAVLVGAAVALYLIFFCPTDCH"
     gene            2390..2890
                     /locus_tag="PKK81_11080"
     CDS             2390..2890
                     /locus_tag="PKK81_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV33470.1"
                     /translation="MADRLFTKILVPVDFSPCSEEAFRLALSFAKSYQAEVLLLHVVD
                     TKSLDALNRLGLAPATEAAKQKKQLHHFARLNARQLLARDDAKGVTIRRLLADGCPFE
                     EIARTARVEGVDLVVMGSYGGAIGGVDKIFFGSTAEKVVRTAGCPVLTVPLPTKRAKV
                     KTGKSL"
     gene            2903..3331
                     /locus_tag="PKK81_11085"
     CDS             2903..3331
                     /locus_tag="PKK81_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNV33471.1"
                     /translation="MGVRKVSDLLSGGRVWLAACFVLALCSQGGAQAEQRIEVTIKDS
                     VFVTKQVPLRLGVATVIAIVNQDQVRHDFGSTMFDGIPTRVESGGIVSYGKQIGGLFL
                     DAKKEAVVRFTMERPGRHEFRCSIHPNMKGELLLLNVEAV"
     gene            3335..4270
                     /locus_tag="PKK81_11090"
     CDS             3335..4270
                     /locus_tag="PKK81_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV33472.1"
                     /translation="MTLIKRVLFATDFSACADRAMGYALAMASAWKAELCVMTVLELY
                     PGMDPDYTVNKMYLDHLRAEASRQLAAVEARAKAAGQPVTVRIETGIPSQAVQTVAHT
                     IGADLLVVGTHGRTGLDHVLIGSTAERVVRMAPCPVLAVKADKGSAAVPAMANIKRIV
                     VPIDLSACSLDALEYAVQFAKPLGASITILHAMEPVAYGLDFSLSHAKEWKEQRAYLE
                     KRLTVLVACVQAQGLQVDHVLKPGLPADSIASYVTQQGYDLMIMGTHGRRGISHVLVG
                     SIAGAMLRHAPCPVLTVRQFNFGADYQRVVPLGEA"
     gene            4299..4523
                     /locus_tag="PKK81_11095"
     CDS             4299..4523
                     /locus_tag="PKK81_11095"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNV33473.1"
                     /translation="MKPKVKKPSAPQSKVRAVSSAVTNPIELPEGMWERISQKAFELW
                     RERGCREGHALQDWLDAETAVMEEIHEARE"
     gene            4507..>5291
                     /locus_tag="PKK81_11100"
     CDS             4507..>5291
                     /locus_tag="PKK81_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33474.1"
                     /translation="MKLANERVVGRSVPVTKELDIILMRLDRLSMTPGFAQQRRIAFT
                     RALQVYVELGSQAPLSPLPEEVGLADLLLYADFYPEDGQLTLIEQLRDVITEHIPEEE
                     RVWLDPLKHSYMDLVELLPQEIGKERRNLRSIGNGRVYEVPADSLGSDAESGQVLFTR
                     LVREPGDPESPLAVPAGPVLMLSAEDAKALYETTGEERREMEVVGGSFELGDWPEFAK
                     RFGHLLLRNFARMRLAALVEAVSEIRYRTTAGAPYFYVMALYE"
BASE COUNT         1008 a         1482 c         1703 g         1098 t
ORIGIN      
        1 tgaacgggaa cgagcgtgtc taccaggaga acatcgagtt cgatatcgcg cgatataagc
       61 cctgcctttt caatggacag ccctgctgag tcaggttatt gagaaagacc gccaccggcg
      121 ttctcgatcg cacgcctccc tgcggccttg ccggacggcc tttctgacca gcctggggat
      181 tgtggtgact ttctttattg ttgtttctca cctgaaaacg ttcaagcgat acgttcatca
      241 ttcgtgaagc gtatctcgtt tctcctgatt ggtttcagcc gtcctctgcg cgtgttaccc
      301 tccggcactc attcaaacac caacacctga cagggtgcgc gatgcagcac tgcatgcgag
      361 acgctgccca ggacgaatcg ggtgatgccc tgcctggcgc gcgaacccac catgaccagg
      421 tctaccccca tcttttccgc ctcgtggaga atcatcgtca ccggcgttcc caggacgctt
      481 gcgctacggg tggtataccc gagtgtccgg agttcggtag ccactccatc cacaaagtca
      541 cgggcatgcc tgagggcttg cgtctccagt ttttccgctg ccgagtcgtc aacgggccag
      601 ggcggacggg tcggcgggag gacagccagc agattgatgt tcaccggctc atggaagggt
      661 tgcttccgga ggaaccgcag cgccgcctcg gcgtccgatt gtccttccag cgggagcaag
      721 acctgtttca agtcgcggag cgagccctgt aggatcagtt tggcgcaggg agcgaggctc
      781 agtatacgat gggagacgct tcccaacagt cgttccttca ccggtcccag tcccctggct
      841 cccatgagaa tgaggtccac atggcgtgac tcagccgtcg cgacgatcat ttccgccggg
      901 gatcctgagg ccaattgttt ggtgaccggc ccgctgtgag gcgggagcag tgaatggatc
      961 ctggtcagca actgctcgcc atcctctttc atgctgcgtt ccagttgcga atacagctcc
     1021 tgggcgactt ccggcaccat catcgggtag gccggtcggg gagcatcgac gacatggagc
     1081 agggtcagct catccgggcg gcgcagatat ttcagcgccc gcacggcttc ataggagtga
     1141 tcggatccat cgacggccaa gagcactttc atgatcgaga ctcctttgct gcgtgcgcgt
     1201 ccggctggag ataccgctga aaccagcgga gcgcctcccg ggcgacctgc tccaacgtgc
     1261 ccggttcctc gaataaatga ctggctcccg ggacaatcac cagctctttc cggcaggtca
     1321 gttgcctgag cgcgtcctga ttcatctcga tcacgggacc gtcgtctccg ccgacaagca
     1381 ggagcgtcgg tgccgtgacc cggctcaggt aggggccggc caggtcgggc cgtccgccgc
     1441 gcgacacgat ggcgccgacc gcctggggtt cacgtgcagc tgcttggagc gccgcgccgg
     1501 ctcccgtgct ggcaccgaaa tacccgaccc gtagtccggc tgtctggcgg tgtgcctgta
     1561 accaacgttc tgccagcagc agccggtcgg cgaggaggtc gatgtcgaag actttgcggc
     1621 gatcgtccgc ctcatccggg gtgaggaggt ccatcagcaa tgtcgcgaac cctcccgttt
     1681 ccagatggcg ggcgacgaac tggttgcgcg gactgaatcg tccgctgccg ctaccgtggg
     1741 caaaggcgat gacacccctc gggtgcgcag ggagacccag gattccatcg agcgtgatgc
     1801 cgtcatggct gattctgacc tggaagatgc tctccgcttc catagattga tgtggatcct
     1861 gtcggttcat ggccctcagg tcgcgcaacg tgaaatctcg gcgaccgtta gtgacaatcg
     1921 gtcggacaga agaagatcag atacagtgcg actgctgctc ccaccagcac agctccgaac
     1981 aagagccatt gcgttctcga catggttcag tcctcttgaa cgctgatgga gcaagaggaa
     2041 tgccatccga gcatcggcac aggggtggac ggcaagggga ggtctaagcg cctgtagctg
     2101 atggcacgat aggagaaatc tctgtcagca tgtgctattt gctacaagcc ataagctctt
     2161 tgttgctctg tggcgttccg ctacagaaca ctggtattgg ctgtagcagg acaccccccg
     2221 gagatgaagt tcaagagcct agggcgcatg gccaatggcc ctgatgtctg caaaatcctc
     2281 tgttccataa gccagaggct ctatgcccct cgctcctttc tgcatggcat cggccttgct
     2341 gatcttgcta atactggtgc tagaggtagg agtgatgagt ggaggtgtaa tggccgatcg
     2401 tctgttcacg aaaatcctcg tgccggtgga tttttctccc tgctccgaag aagccttccg
     2461 gcttgcgctg tcgttcgcaa aatcgtatca ggcggaggtg ctcctgctgc atgtcgtcga
     2521 taccaagagt ttagacgcgc tgaatcgact cgggctggcg cctgccactg aagccgcgaa
     2581 acagaaaaag cagctgcatc atttcgcccg cctcaatgcc cggcaactgt tggccaggga
     2641 tgacgcgaag ggggtgacga tccgccggct gctcgcggac ggctgcccat tcgaggaaat
     2701 tgcgcgcacg gctcgggtgg agggggtcga tttggtggtc atgggaagct acgggggcgc
     2761 gatcggcggg gtcgataaga ttttcttcgg cagcacggcg gagaaggttg tccggacggc
     2821 cgggtgtcct gtattgacag tgccgctccc cacgaaacgg gcgaaagtga aaacgggcaa
     2881 gagtttataa aggaggtcac ttatgggtgt gaggaaggtg tcggacttgt tgagcggagg
     2941 acgggtatgg ttggcggctt gtttcgttct ggccctctgt tcgcagggtg gagcgcaggc
     3001 ggagcagcgg atcgaagtga cgatcaagga ctccgtcttt gtcaccaaac aggtgccgct
     3061 ccggctgggg gtggcgacgg tcattgccat cgtcaatcag gatcaggtgc gccacgattt
     3121 cgggtcgacc atgttcgacg gcattccgac gcgcgtcgag tccggcggga tcgtgtcgta
     3181 tggcaagcag atcggcgggt tgtttttgga tgcgaagaag gaagccgtcg ttcgctttac
     3241 catggagcga ccgggccggc atgagttccg ctgttccatt catccgaaca tgaaaggcga
     3301 actgctcctg ttgaacgtgg aggcggtcta agtcatgacc ctgatcaagc gagtgctctt
     3361 tgcgacggat ttttcggcct gtgcggaccg ggccatgggt tatgccttag cgatggcgtc
     3421 ggcctggaag gcggagctct gtgtgatgac ggtcctggag ctctacccgg ggatggatcc
     3481 ggactatacc gtcaacaaga tgtacctgga tcacttgcgc gccgaggcca gccgtcagct
     3541 ggcggcagtg gaggcacgcg ccaaggcggc cggacagccg gtgacggtgc gcattgaaac
     3601 cggcattccg agccaggcgg tccagacggt cgcccacact atcggcgcgg acctgctggt
     3661 ggtcggtaca catgggcgca ccggattaga tcatgttctg atcggcagca cggcggagcg
     3721 ggtcgtccgt atggcgcctt gcccggtcct ggcggtgaag gccgacaagg gcagtgcggc
     3781 tgtccccgcg atggcgaata tcaaacgaat cgtggtgccc atcgatctct cggcctgttc
     3841 gctcgatgca ttggaatatg ccgtccagtt tgccaaaccg ctcggagcgt cgatcaccat
     3901 tctgcatgcc atggagccgg tggcgtatgg attggacttc agtctgagtc atgccaagga
     3961 gtggaaggag cagcgggcct atctggagaa acgcctcacc gtgctggtcg cctgtgtgca
     4021 ggcgcagggt cttcaagtcg atcatgtctt gaaaccggga ctgcccgcgg attcgatcgc
     4081 atcctatgtc acccagcaag ggtacgattt gatgatcatg gggacgcatg gacggcgtgg
     4141 catctcccac gtcctcgtcg gcagcattgc cggcgcgatg ctgcgccacg cgccctgtcc
     4201 tgtgctcacg gtgcgtcagt tcaatttcgg cgcggactat caacgtgtcg tgcctctcgg
     4261 ggaagcataa ccagcaggct gtcgtaaagg agtcccccat gaagcccaaa gtgaaaaagc
     4321 cgtccgctcc gcaatcgaag gttcgagccg tcagctcggc cgtgacgaat ccaatcgagc
     4381 tgccggaagg catgtgggaa cgaatctctc agaaggcctt tgagttgtgg agggagcgag
     4441 ggtgtcggga gggacacgcg cttcaggatt ggctggatgc ggaaacggcc gtcatggagg
     4501 agattcatga agctcgcgaa tgaacgagtc gtcgggcgat cggtgccggt gacgaaggag
     4561 ctggacatca tactgatgcg gctcgatcgg ctgtcgatga cgccgggttt tgcccaacag
     4621 cgccggattg ccttcacgcg cgcgttgcaa gtgtacgttg aattgggtag ccaggcccct
     4681 ctgtcgccgt tgccggaaga agtcgggctg gccgatttgt tgctctatgc agacttctac
     4741 ccggaagacg gccagctgac cctgatcgag cagctgcgtg acgtcatcac tgaacatatt
     4801 cccgaggaag agcgggtctg gctggatccg ctcaaacatt cctatatgga tctcgtcgaa
     4861 ttgctgccac aggagatcgg gaaggaacgg cggaatctac ggtcgatcgg aaacgggcgc
     4921 gtgtacgagg tgccggccga cagcctggga tcggacgcag aaagcggaca ggtgttgttc
     4981 acgaggctcg ttcgggagcc gggtgatcct gaaagcccgc tggctgtgcc ggctgggccg
     5041 gtcttgatgt tgtcggcgga ggacgccaag gcactctacg aaacgaccgg tgaagagcgt
     5101 cgcgagatgg aggtcgtcgg cggctcattc gagctgggcg actggccgga attcgccaaa
     5161 cggttcgggc atctgctgtt gcggaatttt gcacgcatgc gcctggcggc cctggtcgag
     5221 gcggtgagcg aaattcgtta tcggacgact gccggcgccc cttatttcta tgtcatggca
     5281 ttgtatgaac a
//
LOCUS       DAOQRM010000162         5282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16166_length_5282_cov_17.649130, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000162 DAOQRM010000000
VERSION     DAOQRM010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16166_length_5282_cov_17.649130"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..667
                     /locus_tag="PKK81_11105"
     CDS             77..667
                     /locus_tag="PKK81_11105"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HNV33475.1"
                     /translation="MKALTQQDLIPHDEYERRRETYRQAIIELKRRRRIGLGEKITLV
                     FENRETLRFQIQEMIRVERIFDPQKVQEELDVYNALLPATGELSATLLIELTDPDTMK
                     QWLDLFMGLDHGQKVGLRAGGDVVYGEFEGGHSHDTKISAVHFVRFRPTPAMVTAIGD
                     QAARVALTVRHAGYEAEAEVPWVMRQEWLEDLSTVG"
     gene            741..>1089
                     /locus_tag="PKK81_11110"
     CDS             741..>1089
                     /locus_tag="PKK81_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNV33476.1"
                     /translation="MASVLLNKRIEFCASHRYHKPEWDAAKNRATFGPCNNDPGHGHN
                     YMLEVTVAGEVDQRTGMVVNLFDLKRVLLQVLEEFDHKHLNLDLPYFERQIPTSENVA
                     RVLWDKLQAQPDIG"
     assembly_gap    1090..1189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1508..2752
                     /locus_tag="PKK81_11115"
     CDS             1508..2752
                     /locus_tag="PKK81_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33477.1"
                     /translation="MMSIWEFFGQDVALFGQLHSPMLSWLGSVGLIALFLWHAGRLTA
                     AISSVQGCYTRVWPTLTSLTASRKSLQSEWLTVPSLSDVKKPANESGGQSERVDLDDL
                     HTLDKAMRREPRLEQAWLHFRKTFIVERTAWFIEPKVFATRTAAEFFPRDLLNSRLNL
                     AFYHQFPSLITGGGLLLTFLAILIGLSKLHADGSHIVGIQGLINGLAGKFLTSIVGLL
                     CANLFVLLEKSALHRLGTTQQQFVAMVDELFPRRTMEQMLENFTPGAGPSHTPAAVGA
                     VPVDLGDRLVGTLSDRLSPTVTALREAVEVLSRREPGGRFAAPDRLAEELSRVMQQTM
                     AAPIQELNQAIHLLAHSVEELKRDQKMEPGAEPRESEFESTFFDDTLPSELEEEAIPD
                     DSMSGLRWFANWRQGASMKGAA"
     gene            2754..3506
                     /locus_tag="PKK81_11120"
     CDS             2754..3506
                     /locus_tag="PKK81_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV33478.1"
                     /translation="MRTNFSSDSQEQSSTLTMGITDLMTSLAVIFILLFSAYVTKVSE
                     TESQAKVPVPIPAPERKVARTTTDDIKGLLRDHFQRFDLSLDADPTDPNVVRIVVPEA
                     LLNFEFGRGALSSVADRFLADAMPTYAALLCGAMRDQIDSLVIEGHTDDRGSDIYNLK
                     LSQQRSLNVMVKGLEVIQDSAPWAYRCFHEKTSASGRGRQDLVMDESRGLDRDKSRRV
                     VFKIRLRPTAQQTEMKQAMRTIEEPNFSSRLF"
     gene            complement(3503..4195)
                     /locus_tag="PKK81_11125"
     CDS             complement(3503..4195)
                     /locus_tag="PKK81_11125"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNV33479.1"
                     /translation="MVLIPAGEFAMGSDLGQEDEQPVHRVSVKAFYLDVYETTVSRYA
                     EFLASQKPDAPFKWNEATAGAHENKPAVGVNWYDARDYCRWVGKRLPTEAEWEIAARD
                     TEGRIYPWGSAHPTKGHANAGETRWRGYDSLSNVGRFELGKTPEGVYDLAGNLWEWVA
                     DWYDPTYYQFSARDNPKGPSAGPLRALRGGAWNNDSKAIRSSNRAGYAPDARRNDVGF
                     RCAQDGPSPERR"
     gene            complement(4553..4885)
                     /locus_tag="PKK81_11130"
     CDS             complement(4553..4885)
                     /locus_tag="PKK81_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV33480.1"
                     /translation="MTGTVQDVRDENYKEFTESAGAVVAYGLATCEPCKQYDPILEET
                     AAKFPTIKIGKAKMHVPGRCREIKKAHTFETYPTTHFFAQGKLLLTREGVVEPAELAA
                     LISDHLLK"
     gene            complement(4968..>5282)
                     /locus_tag="PKK81_11135"
     CDS             complement(4968..>5282)
                     /locus_tag="PKK81_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNV33481.1"
                     /translation="GENKCLTDDFPKFQSAHAELFGISCDSFFSHKAWADSLDLKHRL
                     LSDFNREVVKKYGLYFEPLNCGKRATVIVDKNGKVAYVKVQEIKVAREDKDILAALAK
                     LS"
BASE COUNT         1006 a         1424 c         1565 g         1187 t
ORIGIN      
        1 cccagggacc cgcgtgcctc ctgagggttg gcctcctgct cgcggcagga gatgtcttct
       61 caccccggac tagattatga aagcactcac acagcaggac ctgattcctc acgatgagta
      121 tgagcggcgg cgcgaaacgt atcgtcaggc tatcatcgag ctcaaacggc gtcggcggat
      181 cggtcttggc gagaagatca cgctggtctt cgagaatcgg gaaactctgc gtttccagat
      241 ccaggaaatg attcgcgtcg agcggatctt cgacccgcaa aaggtccagg aagaactgga
      301 tgtgtataat gcgcttctcc cagccacggg tgaattgagc gcgaccctcc tgatcgaact
      361 cacggatccc gacacgatga agcagtggct cgatttgttt atgggcctgg atcacggcca
      421 gaaggtcggt ctccgggccg ggggggacgt ggtctatggc gaattcgagg gcggccatag
      481 ccatgacacg aagatcagcg cggttcattt cgtccgcttt cgtccgaccc ctgcgatggt
      541 aacggccatc ggtgatcagg ctgcgcgggt ggcgctgaca gttcgtcatg ccgggtatga
      601 ggccgaagcc gaggtgccgt gggtgatgcg acaggaatgg cttgaggatt tgagcacggt
      661 cgggtaatgt tcatggatgt gtgcgcgatg cgcacccaca ataatcgtgc ctgaacgctg
      721 tcaaacgaga atcggttcct atggcctcag ttctcctcaa caaacgcatt gagttctgcg
      781 cgtcccaccg gtaccacaag ccggagtggg atgctgcgaa gaaccgcgcc acgttcggcc
      841 cctgtaataa cgatcctggg catggccata actacatgct ggaagtgacg gtggcgggag
      901 aggtcgatca gcggaccgga atggtcgtga acctctttga tttgaaacgc gtgttgttgc
      961 aggtgttgga ggagttcgat cacaagcacc tcaatctcga cctgccctat tttgagcgcc
     1021 aaattccaac ctccgaaaac gtcgcgcgtg tcctgtggga caagcttcaa gcgcagccgg
     1081 atatcggtan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ttcaactcgt
     1201 caagctcgtc caaactcgcg atctcttcta cgaatacgcc ggctgattcc ggtccgtctc
     1261 ctctcgtctc cctgcaacgt ggctgagcgt tgtgcctgcg agggccagcc agccgacgac
     1321 tacagaagct gcacaaactt ccaccgctcg gcagttcttg ccgggtgttc tgcgtcgagt
     1381 gctcctcgca ctcgcaaatt cactgggttt caagccgttc ttcatcccgg tatctgtggc
     1441 ccgaaagttg tatctatgga tggatgctgg gtcgttctgc tcgggtcgat cgggaggtac
     1501 tgacaccatg atgtccatct gggaattctt cggccaagac gtggcgctct tcgggcagct
     1561 ccatagtccc atgttgagtt ggctcggttc cgtcggattg atcgcgctgt ttctatggca
     1621 tgccgggcgg ctgacggcgg ccatctccag cgttcaggga tgctacaccc gcgtatggcc
     1681 gaccttgacg agtctcactg ccagccgcaa gagtctgcaa tcagagtggc tgacggtccc
     1741 cagcttgagc gatgtgaaga aaccggccaa cgagtccggt ggtcaatcgg agcgggtcga
     1801 tttggatgat cttcacacgc tcgacaaggc catgcgtcgg gagcctcgcc ttgaacaggc
     1861 ctggctgcat tttcgcaaga cctttattgt ggagcgcacc gcctggttca tcgagccgaa
     1921 agtgtttgcg acgcgcacgg cggcagaatt tttccctcgt gatctactga atagccgatt
     1981 gaatctcgcc ttttaccatc agttcccgtc gttgattacg ggaggcggtc tgttgctcac
     2041 gtttcttgcg attctcatcg gcctgagcaa acttcatgcg gatggatcac atatcgtggg
     2101 cattcaaggg ttgatcaacg gattggccgg gaaatttttg acgtcgattg tcgggttgct
     2161 ctgtgccaac ctctttgtgc ttctggagaa gtccgccttg catcggttgg gcaccactca
     2221 gcagcagttt gtcgccatgg tcgatgaact gttcccgcgc agaaccatgg aacagatgtt
     2281 agagaacttc acccccggag cggggccatc acacacgcct gccgcagtgg gtgcggtgcc
     2341 cgttgatctt ggcgatcgcc tcgtgggtac cctcagtgat cggttgagtc ccaccgtgac
     2401 ggcgctgagg gaggccgtag aggtgttgag caggcgggaa ccgggtggcc ggttcgctgc
     2461 accggatcgt ttggcggagg agttatcgcg ggtgatgcag cagacgatgg cggctccgat
     2521 tcaggaattg aaccaggcga ttcacttgct tgcccactcg gtcgaggaac tcaaacgtga
     2581 ccaaaaaatg gagccaggag cggagccgag agagagcgaa tttgaatcga cgttcttcga
     2641 tgacacgctg ccgtcagaat tggaagagga agccatccct gatgacagca tgagcgggtt
     2701 acggtggttt gcgaattggc gtcagggcgc ctcaatgaag ggcgcggcct gatatgcgca
     2761 ccaacttctc gtcggattcg caggaacagt cttcgactct caccatgggc atcacggatt
     2821 tgatgacgtc actcgccgtc atttttatcc tcctgttcag cgcctatgtc accaaagtct
     2881 ccgaaacgga atcacaggcg aaggtgccgg tccccattcc ggcgccggag cggaaggtgg
     2941 ctcggacgac gacggatgat atcaaggggc tgttgcgcga ccattttcaa cgattcgacc
     3001 tttccctgga tgccgatccc accgatccca atgtggtgcg gattgtcgtg ccggaggcgt
     3061 tgctgaactt tgagttcggg agaggcgcac tctcgtcggt ggcggaccga tttcttgcgg
     3121 atgcgatgcc gacctatgcg gcgttgctct gcggtgccat gcgggatcaa atcgattcgc
     3181 tcgtgatcga agggcatacc gatgatcgcg gctcggatat ctacaacttg aagttgagcc
     3241 aacagcgttc gttgaatgtg atggtgaagg ggttggaagt catccaggac tcggcgccct
     3301 gggcctatcg ctgtttccac gaaaagacgt ctgccagcgg gcgaggtcgg caagatctcg
     3361 tcatggatga gtcgcgtggc cttgatcgag ataagagccg acgagtggta tttaaaatcc
     3421 gcctgcggcc tacggctcag cagactgaga tgaagcaagc catgcggacg attgaagagc
     3481 cgaacttcag ctcccgcctg ttctaacgcc gttccgggga cggcccgtct tgcgcgcaac
     3541 gaaatccgac atcattccgt cgcgcatctg gcgcatatcc ggcgcgattt gacgacctga
     3601 tggccttgga atcattgttc caggcgcctc cacgcaatgc ccgaagtggc cccgctgaag
     3661 gtcctttcgg attgtctcgt gcgctgaatt ggtagtaggt cggatcatac cagtcggcga
     3721 cccattccca gagattccct gccaggtcat acaccccctc cggtgttttg cccagttcaa
     3781 agcgtccgac attgctcagg ctgtcgtatc ctcgccatct ggtctcacct gcattcgcat
     3841 gtcctttggt cggatgcgca cttccccacg ggtagatccg tccctcggtg tcgcgagctg
     3901 caatctccca ctccgcttct gtcggcaatc gtttccccac ccatcggcaa tagtcacgcg
     3961 catcgtacca attgacgccg accgccggtt tgttctcgtg agcgccggcg gtggcctcat
     4021 tccacttgaa cggcgcatcc ggtttctggg aggccaggaa ctcggcataa cgagagacgg
     4081 tggtttcgta cacatcaaga taaaaggctt tgaccgagac gcgatggacc ggctgttcat
     4141 cctcctgacc gaggtcgctg cccatggcga attcaccggc aggaatgagc accattggcc
     4201 caccacgatg cgagccgatg aacttggtgc taggctcgac gggggcggag ttggatggat
     4261 tagctacgac tgaatgtgaa gggcgtggtg aaatgtcctt cgcttcggca gctcctgtga
     4321 atgggaggca atagagcagc gcgatgacac aggcgatcgg tacgaatgtc tgcattgttt
     4381 cgtgggtgat cgactgcgag acggtcggcg gttttggtct cacctgcaca ttgtatcccg
     4441 ttcatgcgaa aaagtgtggg ggagggaccg tgtagggtgc gcgtccttag cgacggacaa
     4501 gttgcagcag ggcttcagtg ggggcggtga gggctatcgg agatggaggc tgttacttca
     4561 gcagatggtc ggagattaat gccgccagtt cggccggttc gaccacccct tcacgagtca
     4621 gcagcagttt gccctgcgcg aagaaatgcg tggtggggta ggtctcaaac gtatgcgctt
     4681 tcttgatctc tcggcatcgg cctggcacgt gcattttggc cttgccgatc ttgatcgtgg
     4741 ggaactttgc agcggtttct tccaggatgg gatcgtattg cttgcacggt tcgcaggtgg
     4801 ccaagccgta ggcgacgact gcgccggcac tctcggtaaa ctctttgtaa ttttcgtcgc
     4861 gaacgtcttg gactgtgccg gtcatactct ctcctcggct gtgagggatg accgaggggt
     4921 gggcgtcgtg gtgacctgac cccttccccc tcggtccctc gtggcgctta gctcagtttc
     4981 gcgagggccg cgagaatgtc cttatcttcc cgtgccacct tgatttcctg aactttcacg
     5041 taggcgactt tgccgttctt atcgacgatg accgttgcgc gcttgccgca attcaacggt
     5101 tcaaaataca gtccatactt cttcacgact tcgcgattga agtcagagag caggcgatgc
     5161 ttcaagtcga gggaatccgc ccaggccttg tgggagaaga agctgtcgca gctgatgccg
     5221 aacagttcgg cgtgggcgct ctggaacttc gggaagtcat cggtcaggca cttgttttca
     5281 cc
//
LOCUS       DAOQRM010000163         5260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16278_length_5260_cov_19.208453, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000163 DAOQRM010000000
VERSION     DAOQRM010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16278_length_5260_cov_19.208453"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PKK81_11140"
     CDS             complement(<1..482)
                     /locus_tag="PKK81_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33482.1"
                     /translation="MTVLTNGKQSHVLTNDFPHSLNRRSADRASVLLIFCVIATMFSA
                     LSSVADAAPPEHPFSLEFETGAVWQSRNQIQIPDSNAGTRFALTDIQGHGPEAQRRVE
                     LTWNMNHRHSLRFVYAPLGFSGTGAFGQQVRFAGSTFAPGSPIDSEYKFDSYRLTYRY
                     L"
     gene            complement(466..1032)
                     /locus_tag="PKK81_11145"
     CDS             complement(466..1032)
                     /locus_tag="PKK81_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV33483.1"
                     /translation="MISVMDACPTLHTERLLLRPFKAADADTVYRLAGVKEVAAGTLL
                     PHPYDLEAAAQWIAQQQESFAASNAITFAIVLAEEERLIGSIGMEIAHEHQLARLSYW
                     LGTNYWNQGYCTEAVRAVLDYGFTRLSLHRIYAPHFHNNPASGRVLRKVGMTYEGRMR
                     EHYIRFGQFVDVEIYGMLREEFLNDSAH"
     gene            1218..1706
                     /locus_tag="PKK81_11150"
     CDS             1218..1706
                     /locus_tag="PKK81_11150"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNV33484.1"
                     /translation="MTAFNPLAALRSPRETLDGYIILPRLIDKVRVQARGELPAAYQR
                     NLLHRGGTLDGRFLAFTGLEGEALRAVILSCETDEPVAQWVAQHAARHSADEKQRWAA
                     EVEGYRPTGRVLLYLQSTVPELAAQIDCALVSLLDLIDMDEGRLAVPARGGSADVEER
                     SA"
     gene            1684..2319
                     /locus_tag="PKK81_11155"
     CDS             1684..2319
                     /locus_tag="PKK81_11155"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNV33485.1"
                     /translation="MLRKEAPEWYAPLGDDFGPEQMRGEIAVAQRYKELSKTHIEFIT
                     TQKLFFVGTATAESRVNVSPKGMDSFRVLGPARVAWLNVTGSGNESSAHVQHDPRMTI
                     MFCAFEGQPLVLRLYGRAKVVHRGDSEWQEFFGLFPPLPGARQIFDVTLDLVQTSCGM
                     AVPYLSYLGDRESLSAWAEKKGDEGLRRYREEKNQLSLDGIPTHIVDKEGH"
     gene            2444..2650
                     /locus_tag="PKK81_11160"
     CDS             2444..2650
                     /locus_tag="PKK81_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33486.1"
                     /translation="MDVAQVLIAIVGLGAAVTTYCLMRFPHMRRERFRHYGAFSIAVS
                     VLGALAVLLFCLALLILQNAWRVG"
     gene            2958..>5260
                     /locus_tag="PKK81_11165"
     CDS             2958..>5260
                     /locus_tag="PKK81_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33487.1"
                     /translation="MADYFLRDVETIMGLVRTGHSLQLLPRREAEAQADCLEFPRLSF
                     KPPPHPVSIDRVDWVFAGLLALSVAILCLLVARSMPSFLFQSMDFWFEADTLREISNM
                     SRVHDDHYRTSVHPLFSLITFIPVYIAKHALTTSPLRAVLLITGVVGGLWAGTLYLLF
                     RLLGCRRLDASVFTLLGLCSASALFWLPVPNSYSWGSLSIMLALVLLLFAEQRTFGAT
                     AYVIASALTLSFTVTNWMAGLLVTLVRWPWKQAVQLSVNALCVVVLLWGVQKLIFPTA
                     EFFIGHREEAEFINHPQSGGAANVLSSLVFHTVLAPDVRFMKDDAYTQAKSDSFRLSE
                     RLTFQFSRPGSTGPLGLLAVGLWSALLLNGLWRLVTLDHHLRFRLVLAGLLGFETLLH
                     LLYGEETFVYSLNFLPLLLAVAALGAISPGRRVVVSLAALLALCAGLNNWHQFQEATR
                     SATQFTPQRELMTTMMQQDPDRPWPRSVGHVPLALPGTPEGGTAYHEPGGDFSPHVPS
                     FGVSIWLCDANGLPVVTSQTVPLSDIQQRFAPSTHPNIPAIVTKTPYYQATWSRLDAT
                     HWELRLKNYTNHVPAILIRSVGPAGGPVTALDWDGEQIGINHRWAVKATPAPGAITLG
                     DENTPDWMTAQSSARSWSGESGWGYARMKFASASTRKDDEVRVVLADLQPPVEIQTHY
                     THPSKRARLALPEPRLQASMDAQITHLMMSLVDDETRPGDPTFYYRAWHRQGSYITAA
                     LARAGDPHVSRVLSQFLASHDFAGGSGP"
BASE COUNT          926 a         1537 c         1625 g         1172 t
ORIGIN      
        1 aggtatcgat aggtgaggcg gtaggagtcg aacttgtact cggaatctat cgggctgccg
       61 ggggcaaacg tgctgcctgc gaagcgcact tgctgtccga atgcgccggt cccggaaaat
      121 ccgagcgggg cgtacacgaa gcgaagtgag tgccgatggt tcatgttcca ggtcagctcg
      181 acgcggcgct gggcttccgg accatggcct tggatatcgg tcagcgcgaa cctcgtcccg
      241 gcgttgctgt cgggaatctg aatctgattg cgcgattgcc acactgctcc ggtttcgaat
      301 tccagggaga agggatgctc aggaggcgcg gcgtctgcga cggaggacag agcgctgaac
      361 atcgtggcta tgacacagaa tataaggagg accgatgccc ggtcggcaga gcgtctgttc
      421 aagctgtgcg ggaagtcatt cgtgagcacg tgcgactgct tcccgttagt gagcactgtc
      481 attcagaaat tcctctctca acatcccata gatctccaca tccacaaatt ggccgaagcg
      541 gatgtaatgc tcgcgcatgc ggccttcgta ggtcatcccg acttttcgta agacacgtcc
      601 cgaggccggg ttgttgtgaa agtgcggggc atagatgcgg tggagtgaca ggcgggtgaa
      661 gccgtagtcg agcaccgccc gaaccgcttc ggtgcagtag ccctggttcc agtagttggt
      721 gccaagccag taactcaggc gcgccagttg gtgctcgtgg gcgatttcca tcccgattga
      781 accgatgagt cgttcctctt cagcgaggac gatggcaaat gtgatggcgt tgctcgcggc
      841 gaaactctcc tgttgctgag cgatccattg tgcggccgct tcgaggtcat agggatgggg
      901 gagcaacgtg cctgcggcga cttccttcac ccctgccagg cggtacacgg tatcggcatc
      961 ggcggccttg aatggtcgga gaagcagccg ttcagtatgg agtgtcggac aggcatccat
     1021 cacgctgatc acagcatagt atccagccta aaacaattgc cgagttcggc gtcgaccgcg
     1081 atgcgcgtca tgaacgcctg tcaattgaca gcgcctctgc acccggccag aatcctgatt
     1141 gtcggaacag gcaaaagaaa tgcttcggtc ttgcttccgg aatatcgaca ggtcgaaacg
     1201 gagggaggca ggtggtcatg acggcgttta atccactcgc tgcgctgcgc tctccgcggg
     1261 agacgttgga tgggtacatc atcctcccgc ggttaatcga caaagtgcgc gtgcaggcac
     1321 gaggcgagct tccggcggcg taccagagga atctgctgca tcgcgggggc acgctcgacg
     1381 ggcgcttcct tgcttttacc gggctggaag gggaggcctt gcgggcggtc attctctcct
     1441 gcgagacgga tgagccggtg gcgcaatggg ttgcccaaca tgcggcacga cattccgccg
     1501 acgagaaaca gcggtgggcc gccgaggttg agggttatcg gccgactggc cgtgtccttc
     1561 tttacctgca atcgaccgtg cctgaactgg ccgcccaaat cgattgtgcc ctcgtgagcc
     1621 tgctcgatct catcgatatg gatgaaggcc ggcttgctgt ccccgcacgg ggtggctctg
     1681 cagatgttga ggaaagaagc gcctgagtgg tacgcccctc tgggcgatga tttcggtccc
     1741 gaacagatga gaggggaaat tgcggtggct caacgataca aggaactttc aaagactcat
     1801 atcgaattca tcaccacaca gaagttgttc tttgtcggaa cggccacggc ggagagccgg
     1861 gtaaatgtgt cgccgaaagg catggactcg tttcgggtgc tcggccccgc tcgcgtggca
     1921 tggctcaacg tcaccgggag cggtaacgaa tcctcggccc atgtgcagca tgacccgcgt
     1981 atgacgatca tgttctgcgc ctttgagggc cagccgctgg tcctgcgcct ctatggcagg
     2041 gcgaaggtgg tgcacagggg cgactccgaa tggcaggagt tttttggtct gttcccgccg
     2101 ttgcccggtg cccgtcagat tttcgacgtg actctcgacc tcgtgcaaac gtcctgcggc
     2161 atggcagtgc cctatttgtc gtacctcggc gaccgggagt cgctgagcgc ctgggctgag
     2221 aagaaaggcg acgaagggtt gaggcggtac cgggaagaaa agaatcagct gagtctggac
     2281 ggtattccca ctcacattgt cgacaaagaa gggcactgat cccttctcga tcagcatcat
     2341 acgcatccgc ccggaacgca gtcccgcaca cttttcctcc gtttcttgac gcatttccga
     2401 ttcacgagta gggtttcgtt ggccttctac gtaagtggaa acaatggacg tggcgcaggt
     2461 cttgatcgcg atcgtcgggc tgggagcggc tgtgacgacg tattgcctga tgcgatttcc
     2521 ccacatgcgc cgtgagcggt tcaggcacta cggggcgttt tctattgcgg tgtcggttct
     2581 cggtgcactg gcggtcttgc tgttctgcct ggccctgctc atcctgcaga atgcctggcg
     2641 agtcgggtga cgcgagggac aattcatgcc tctcattctc ggaacgatcg caaatcggcc
     2701 tgttccaaag tgaggcattg tcgcagcggc gcgacagttt cgtgcgcagg atcatgaaaa
     2761 tcccaccatg cgagcaactc ttcgccggcc gaggttgatc tggccgggtc ggtctgctac
     2821 gaatactaga gtgcggcatc tggcaggaag ctcatcgttt ttccccaact gagcgtcctt
     2881 tcctcacgtc gagcccatca gcgggttcga gtcgttccat tacaatcgac cgttccccgc
     2941 gcggctgtgt gagacgcgtg gcggactatt ttttgcggga cgttgaaacc attatgggcc
     3001 ttgtcagaac cggtcattcc cttcagctgc tgccacggcg tgaagctgag gcacaggcgg
     3061 attgccttga gttccccagg ttgtccttca agccgccccc tcacccggtc tccatagacc
     3121 gggtcgattg ggtctttgcc gggttgctcg cactgtccgt ggcgatcctg tgcctgctgg
     3181 tcgcgcgatc catgccctcg ttcctgttcc agtcgatgga tttctggttc gaggccgaca
     3241 cgctgcgcga aatctcgaac atgagccggg tgcacgacga ccactatcgg acgtccgtgc
     3301 atccgttgtt ttcgttgatc accttcatcc cggtctatat tgcgaagcat gcgttgacca
     3361 ccagcccgct tcgcgcggtg ttgctcatca ccggcgtggt gggcggcctg tgggccggga
     3421 cgttgtacct gctgttccgc ttgcttggtt gtcgcagact ggatgcctcg gtcttcacgc
     3481 tgctcggcct ttgtagcgcc tcggccctgt tctggcttcc ggtgcccaac tcctattcct
     3541 ggggatcgct ctcgatcatg ctggccctcg tgctgctgtt gttcgccgag caacggacgt
     3601 tcggagctac ggcctatgtg atcgcgagtg cactgacgct cagctttacc gtgacgaact
     3661 ggatggcggg tctgctggtg acgctggttc gatggccctg gaagcaggcg gtgcaactct
     3721 ccgtcaatgc gttgtgtgtg gtggttttgc tctggggcgt gcagaagctc atctttccca
     3781 cggccgaatt ttttatcggc catcgcgaag aggcggagtt catcaatcat ccccaatccg
     3841 ggggtgccgc caacgtcctg tcgtccctgg tcttccacac ggtgcttgcc ccggatgtcc
     3901 ggttcatgaa agacgatgcg tacacccagg ccaagtccga ttcgtttcgc ctgtccgaac
     3961 gtctgacctt tcaattttcc cgcccgggat cgactggtcc actggggctg ctggccgtcg
     4021 gactctggtc ggcgttgctg ttgaatggat tgtggcggct ggtcaccctc gatcaccatc
     4081 ttcggttccg cctcgtcctg gccggcctgc tggggtttga gactctgctg cacctcctct
     4141 atggagaaga aaccttcgtc tacagtctga actttcttcc gcttctcctc gccgttgccg
     4201 ccttgggggc catcagtccc ggccgtcggg tcgtcgtgtc cctggccgcg ttgctcgcgc
     4261 tctgtgccgg gctgaacaac tggcatcagt ttcaggaggc cacgcgttcg gcgacgcagt
     4321 tcacgccgca gcgtgagctc atgacgacga tgatgcagca ggatccggat cgaccctggc
     4381 cccgctcggt ggggcatgtg ccgctggcgc tgcctggaac tcccgagggc ggcacggcct
     4441 atcacgagcc gggcggggat ttcagtccgc atgttccgag cttcggcgtc tcaatctggc
     4501 tctgcgatgc caacgggctt ccggtcgtca ccagccagac ggtgccgctc agcgatatcc
     4561 agcagcggtt cgcaccctct acgcatccga acattccggc cattgtcacg aagacaccct
     4621 actatcaggc gacctggtcg cgtctggacg ccacgcattg ggagctgcgg cttaaaaact
     4681 atacgaacca tgttcccgcg attctcattc gcagcgtggg tccggccggc ggacccgtaa
     4741 ccgcgttgga ttgggacggg gaacagatcg ggatcaacca tcgttgggcg gtgaaggcga
     4801 cgcctgctcc cggcgcgatt accttggggg atgagaatac gcccgattgg atgaccgcgc
     4861 agtcatcggc caggtcctgg agcggtgagt cgggatgggg gtacgcgcga atgaagtttg
     4921 cgtcggcctc aacgaggaag gacgatgaag ttcgtgtggt gcttgccgat ttgcagccgc
     4981 ccgtcgaaat ccagactcac tacacacatc cctcgaagcg ggctcgtctc gcgctacccg
     5041 agccacgcct gcaggcctcc atggatgcgc aaatcacgca tctcatgatg agtctcgtcg
     5101 acgatgaaac ccgccccggc gatcccacgt tttactaccg tgcctggcat cgacagggtt
     5161 catacattac ggcagcgctg gcgcgggccg gcgatcctca tgtcagccgg gtcctttcac
     5221 aatttctggc ctcgcatgat tttgccggtg gctccggccc
//
LOCUS       DAOQRM010000164         5217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16492_length_5217_cov_13.659047, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000164 DAOQRM010000000
VERSION     DAOQRM010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16492_length_5217_cov_13.659047"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKK81_11170"
     CDS             <1..242
                     /locus_tag="PKK81_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNV33488.1"
                     /translation="PEDLTPRELEILRLIWDGHTNRSLAEQLNISMKTADTHRSNMMK
                     KLRACNTAQLLKAALEGNLLETDPGLRRPGRGGQR"
     gene            complement(326..562)
                     /locus_tag="PKK81_11175"
     CDS             complement(326..562)
                     /locus_tag="PKK81_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33489.1"
                     /translation="MSGQSCLIDGCQARVYAPAGTHAICKDHFLGFLTWRRRRGPQMF
                     RKYAGMTMDERDLIAGEWLQTLGTKDLPHPIPGL"
     gene            815..1069
                     /locus_tag="PKK81_11180"
     CDS             815..1069
                     /locus_tag="PKK81_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33490.1"
                     /translation="MAMQVISVIGALMVLVAYGLIQAGTWRELDAGYLALNILGSLLL
                     GIVAIEDRRVGFVLLEFAWAGIALVGVARAIRARRAARSL"
     gene            1124..1606
                     /locus_tag="PKK81_11185"
     CDS             1124..1606
                     /locus_tag="PKK81_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33491.1"
                     /translation="MAGLLSADEIFEKAKDAAVGATNADEKALQIDYEALQLKFQAAL
                     GDRKVARCHINKFLPEGYEDQGRFNLVLLTAGNVIFDIVIGDSYFRYDVVSVGQLDKV
                     QVIDAMWDNREKRREEPFLSLRLMHAEETHLLLALDDDERKSLLGFASAVSAVRNPEK
                     "
     gene            complement(1786..>2588)
                     /locus_tag="PKK81_11190"
     CDS             complement(1786..>2588)
                     /locus_tag="PKK81_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNV33492.1"
                     /translation="RVYVQADAVWVEDVGEVGEATFQALRKFLLYGTKAKMENCGESW
                     GLLLVSGPKSAEAVAAAFGIEVRALQLLHTLPATIGGQQALILRTEETGEQDFEVLLP
                     ADAVPAAWNQLMTSGAPFGIKPVGAQARELLRIEAGLPKAGPDLNEEIVPPEANLEGK
                     AFSLSKGCYPGQEVVARMDTYGNVRRHLVGLIIQDKTVPPAGSKLFSGDREVGWVSSA
                     VFSPQRNAALAFGFPLRDFSAPDTTLTVEVAGTRHQATVHALPFQKHS"
     assembly_gap    2589..2688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2689..2954)
                     /locus_tag="PKK81_11195"
     CDS             complement(<2689..2954)
                     /locus_tag="PKK81_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HNV33493.1"
                     /translation="MKQSRLHDQHQQLGAIFEDTAGWSMPAHYGDPAAEYAAVRDAVG
                     LSDLSHRGKIRVTGDDRIKWLQSIISNDILPLQPGQGRYSSFLT"
     gene            complement(2975..3388)
                     /locus_tag="PKK81_11200"
     CDS             complement(2975..3388)
                     /locus_tag="PKK81_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNV33494.1"
                     /translation="MNTTILVAVTDIFFYTKVRDALRPQGYTLERIRSQDEVAEKTAS
                     ASPAALILNMNDLAIDAFKALETLQADPALRSLPILAFANHEETDTWRRAKELGVTKI
                     VSRNEFSARTRELVEDIIANSSQQSPVRSELNAEG"
     gene            complement(3410..3925)
                     /locus_tag="PKK81_11205"
     CDS             complement(3410..3925)
                     /locus_tag="PKK81_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV33495.1"
                     /translation="MDVQDFLIQGDGSEEDKREAWQLFQQAYEQQMKGQLEEAVSLYK
                     LSLATHQTAEAYTFLGWTYSFMGKLDEAIDECHKAIACDPHFGNPYNDIGAYLIEKGD
                     LEEAIPWFQKAMQARRYESPAFPHLNLGRVYERQGKWSEAIDCYKQALALNPDYALAK
                     KALGRLISSLN"
     gene            complement(4005..4814)
                     /locus_tag="PKK81_11210"
     CDS             complement(4005..4814)
                     /locus_tag="PKK81_11210"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HNV33496.1"
                     /translation="MGKPKPAPLRERDITRQIAREYYKEFDQLIESDVIIVGAGPSGL
                     ICAHDLGRMGIKTLIVEQSLALGGGFWSGGYLMNKATICAPAHKILKEVGVPCKQIKE
                     CPGMYMVDPPHATGALIAAAYNAGAKIMNLTRVVDLILRREGVLEGVVVNSTTAEMAG
                     HDIIHVDPIALESKIVVDATGHDAVVVNLLHKRGLYQQVPGNGAMWVSRSEEEVMDRT
                     GEVSPNCFVIGLAVAAVFGTPRMGPAFGSMLLSGRYGAELIQKKLKQGKSI"
     gene            complement(4954..>5217)
                     /locus_tag="PKK81_11215"
     CDS             complement(4954..>5217)
                     /locus_tag="PKK81_11215"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNV33497.1"
                     /translation="EFRWEDQFNLSLDPDTARSYHDATLPDNAAKVAHFCSMCGPHFC
                     SMKITQDVRDYAAQKQLEEQQAIQIGMKEKSEEFKKAGSEIYL"
BASE COUNT          978 a         1486 c         1533 g         1120 t
ORIGIN      
        1 ggccggagga tctcacgccg cgtgagttgg aaattcttcg cctgatctgg gatggtcata
       61 ccaatcgaag tctcgcagag cagctgaaca tcagcatgaa gaccgccgat acccatcgct
      121 cgaacatgat gaaaaagttg cgcgcgtgta atactgccca attacttaaa gcggcgctgg
      181 agggaaatct gctagagacc gacccgggcc tgaggcgtcc cggtcgggga ggccaacgct
      241 gagggccgac agccaggaga ccggtttgaa ttgcccgacc ttccgtccgg tgcaggacag
      301 gaaggtgcag gtgaagcggg gagacttaca agccgggaat ggggtggggc aggtctttgg
      361 tgccgagggt ctggagccac tcacccgcga tcagatcgcg ttcgtccatc gtcatgccgg
      421 catatttacg aaacatctgt gggccccgtc gccgcctcca cgtgaggaag ccgagaaagt
      481 gatccttgca gatggcatgg gtgcctgccg gcgcatacac tctcgcctga catccgtcga
      541 ttaaacagct ctgtccgctc atcggtatcc cccctgattg ccccaaagta tgccggagat
      601 cgacctggaa atgcaatgtg gtggcccggc gagcgcagcc tgccagacag gccgacgcac
      661 cgcgccggag tgatgctcaa ccacatcggc accaacccat cagcgtctcc ttgcccgctt
      721 cttcatgggg agtttccgct tgcaatcaca tccagcagcc aggtaccgta ccccgttctc
      781 acgcggcgtc cggcaaggag tgcccgagca taccatggcg atgcaggtca tatcggtgat
      841 cggagctctg atggtgctcg tggcctatgg cctcattcag gccggcacct ggcgtgaatt
      901 ggacgcgggg tatctggcgc tgaacatcct gggctcgttg ctgctgggaa ttgtggccat
      961 cgaagaccga cgggtgggtt ttgttctgct ggaattcgcg tgggccggca ttgcgctggt
     1021 cggggtcgcc cgggcgatcc gcgccaggcg ggctgcgcgt agcctctaaa tcgcccatac
     1081 acaataaaca tcgggcagtc ttgtaacgaa ggagtaggga actatggcag ggttgttgtc
     1141 agcagacgag atctttgaaa aggcgaagga cgcggcggtc ggggcgacca atgccgatga
     1201 aaaggcgctc caaatcgatt acgaagcatt gcaactcaag ttccaggcag ccttgggcga
     1261 tcgcaaagtg gcgcggtgcc atatcaataa gttcctgccc gaagggtacg aagatcaggg
     1321 gcggttcaat ctagtgctgc tcacggccgg caacgtcatt ttcgacatcg tcatcgggga
     1381 ttcctatttc cgctacgatg tggtgtccgt cggtcagctg gataaggtgc aggtcatcga
     1441 tgccatgtgg gataaccgcg aaaagcgccg ggaagaaccc ttcttgagcc tccggctgat
     1501 gcacgccgaa gagacgcatc tgttgttggc attggatgac gacgagcgga agagcctgct
     1561 cggattcgcg tcagccgtgt cggccgtgcg taatcctgaa aagtagaaca cctctgccac
     1621 tcgaaaaagg gtggtcatcg gttcgtgtga ttgaagcccc gcagggtgtt caaaacggcc
     1681 gttcaggagg gccgcagctg gtgaagcact gatgcgtgcg ctcagtggta cgttgaggcg
     1741 ctgaatgacc cgagaacgag gccggcgaac gttttcagca tcctgctacg agtgcttctg
     1801 aaacggtagg gcatggacgg tagcctgatg gcgcgtccct gccacttcga ccgtcagtgt
     1861 ggtgtcaggt gccgaaaagt cgcgtaatgg aaaaccgaat gccagtgcgg cattgcgttg
     1921 cggcgaaaag acggcgctgc tgacccatcc cacctcccga tctccgctga ataacttcga
     1981 gcccgcgggc ggaaccgttt tgtcctggat gatcaatccg accagatgcc ggcgcacgtt
     2041 cccataggta tccatcctgg ccacgacttc ctgtcccggg taacaaccct ttgagaggct
     2101 gaacgccttg ccttccaggt tggcttccgg cgggacaatc tcttcgttga gatccggacc
     2161 ggccttgggc agaccggctt cgatgcgcaa cagttcgcgc gcctgtgcgc cgacgggctt
     2221 gatgccgaac ggtgcgcctg atgtcatgag ctggttccag gcggcgggga ccgcatcggc
     2281 cggcagcaag acttcaaagt cctgttcgcc ggtttcttcg gtacgcagga tcagcgcctg
     2341 ctgcccgccg atggtggcgg gcagggtatg caagagctga agggcgcgga cctcgatacc
     2401 gaatgccgca gcaacggcct ctgctgattt cggtccgctc accagcaaca aaccccagct
     2461 ttctccgcaa ttctccatct tggccttggt gccgtagagc aggaattttc gcaacgcctg
     2521 aaatgtggcc tcgcccacct cgcccacatc ttcgacccac acggcatcgg cttgcacata
     2581 tacccggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ggtcaggaag
     2701 ctggagtagc gcccctggcc gggttggagc gggaggatgt cgttgctgat gatgctctgg
     2761 agccacttga tgcgatcgtc gccggtcacc cggatttttc cgcggtgcga caggtcggac
     2821 agcccgacgg cgtcgcggac ggcggcatat tccgcggcgg gatcgccgta atgggccggc
     2881 atcgaccatc ctgcggtgtc ctcgaaaatg gcgcccagtt gttgatgctg atcgtggagt
     2941 cgggattgtt tcattggatt cttgctctct gctctcagcc ttcagcgttc agctctgagc
     3001 ggaccggtga ttgctgactg ctgttggcta tgatgtcttc aaccagttct ctggttcgtg
     3061 cggagaactc atttctggag acaattttcg tcacgcccag ttccttggcg cggcgccagg
     3121 tgtccgtttc ttcgtgattg gcaaaagcca ggatgggaag cgaccgcaac gcggggtcgg
     3181 cctggagggt ttccaacgcc ttgaaggcat cgatggccag gtcgttcatg ttgagaatca
     3241 gggcggcagg cgaagctgag gcggtttttt cagccacctc atcctggctg cggatccgct
     3301 ccagcgtgta gccctgcggc cgcaaggcat cgcgtacctt ggtgtaaaag aaaatgtcag
     3361 tcacggccac taaaatcgtg gtgttcatcc ggttcctgtc ctatggtccc taattcagtg
     3421 agctgatgag ccgacccaga gccttcttgg ccaaggcgta gtccgggttc agggcgagcg
     3481 cctgtttgta gcagtcaatc gcctcgctcc acttgccctg gcgttcgtag acacggccaa
     3541 gattcaggtg tgggaaagcc gggctctcat accgtctggc ctgcatggct ttttgaaacc
     3601 agggaatcgc ctcctccaga tcaccctttt caatcaggta ggccccgatg tcgttgtagg
     3661 gattcccgaa gtgcggatcg caggcgatgg ccttgtggca ttcgtcgatc gcctcgtcca
     3721 gcttacccat gaagctgtag gtccatccga ggaacgtata ggcttcggca gtctgatgcg
     3781 tcgcgagcga cagtttgtag agactgaccg cttcttccag ctgccccttc atctgctgct
     3841 cgtaggcttg ctgaaagagc tgccaggctt cgcgcttgtc ttcttcgctg ccgtcgcctt
     3901 gtataagaaa gtcctgaaca tccatgtgtg atcacaagag ccaatggcct atggagcata
     3961 gcagttcgat ggtctgttcc ctgctaccag ccgttcgcta tcagctatat gctcttgcct
     4021 tgtttcagtt tcttttgaat taactccgcg ccatagcggc cggagagcag catcgatccg
     4081 aaggccgggc ccattcgtgg tgtgccgaac acggccgcga cagccaaccc gatcacaaag
     4141 caattgggag agacttcccc ggtccggtcc atcacttctt cttccgaccg cgacacccac
     4201 atcgcgccgt tgcccggcac ctgctggtag agcccccgct tatgcaggag attgaccacc
     4261 acggcatcat gtccggtggc atcgaccacg attttactct ccaaggcgat cggatcgaca
     4321 tggataatat cgtggcccgc catttcggcg gtggtgctgt tgacgacgac gccttcaagc
     4381 acgccttcgc ggcgcaggat cagatcgacg acccgcgtca ggttcatgat cttcgcgccg
     4441 gcgttgtagg cggcggcaat cagcgccccg gtggcatgcg gaggatcgac catgtacatg
     4501 ccggggcatt ccttgatctg cttgcaaggc acccccactt ctttgagaat cttatgcgcg
     4561 ggggcgcaga tggtggcttt gttcatcaaa tacccgccgg accagaagcc gccgccgagg
     4621 gccaagctct gctctacgat gagggtcttg atgcccatcc ggccaagatc gtgggcgcaa
     4681 atgaggccgg acggcccggc gccgacgatg atgacgtcgc tctcgataag ctgatcgaac
     4741 tctttatagt actcccgcgc aatctggcgg gtgatatcgc gctcgcgtaa tggcgccggc
     4801 ttcggctttc ccatgctacc ttccctctca gcggctgctg aacatgactc ccggcttcgt
     4861 tctcggctcc atcaaatcct caacgtaccc cggagggtac gcctccggtt tgagttcgcc
     4921 tgtggccttg ccggatagcc atgttgatca gccttataga tagatttctg aaccggcttt
     4981 tttaaattct tctgattttt ccttcatgcc gatctggatg gcctgctgct cttccagctg
     5041 tttctgcgcg gcatagtcgc ggacgtcctg cgtgatcttc atcgaacaga aatgcggtcc
     5101 gcacatgctg cagaaatgcg cgaccttcgc ggcattgtcc ggcagcgtcg catcgtgata
     5161 cgagcgggcg gtgtccgggt ccagcgacag gttgaattga tcttcccagc ggaactc
//
LOCUS       DAOQRM010000165         5180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16680_length_5180_cov_14.352976, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000165 DAOQRM010000000
VERSION     DAOQRM010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16680_length_5180_cov_14.352976"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKK81_11220"
     CDS             <1..591
                     /locus_tag="PKK81_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV33498.1"
                     /translation="NGSGKSTFVKVLTGLYLPQQGEVRLNGEAITPATQDWYRQHFAA
                     VFSDFYLFKKLLGLDPALIESQADGWLKTLRIQHKVTIRDGEYSTINLSQGQRKRLAL
                     VTAMLEDRPFYVFDEWAADQDPQYKEVFYGELLPELRARGKGVIVVTHDDRYFHVGDR
                     VLKLDEGKILEIPNSTASTGSHRATPVFKPVIRSSA"
     gene            623..1387
                     /locus_tag="PKK81_11225"
     CDS             623..1387
                     /locus_tag="PKK81_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNV33499.1"
                     /translation="MSGLLIPSRDSSPSPALSREDVHVWFCRLARHDGRRAALAALLS
                     RDEQTRAARFAFERDRHRFILSHGLLRAILARYVGREATQIEFATGAHGKPALSGQSC
                     AVQDIQFSLSHSGAYAVVAVAAGRAVGVDVEVRRPDVDALKLAERFFAAGEAQQIIQA
                     QGDAQQRLFYRSWTAKEAYLKGRGVGLSLGLDRFEILFDDGSPLAHVRMTDSGALDTN
                     WQVQSLSLADDVSGALAVEGQAWQVRMCDSSTDLFR"
     gene            complement(1384..1872)
                     /gene="tadA"
                     /locus_tag="PKK81_11230"
     CDS             complement(1384..1872)
                     /gene="tadA"
                     /locus_tag="PKK81_11230"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNV33500.1"
                     /translation="MLPLDDLDTRFMQQALTLARSAPLIGEVPIAALLVHEGIVIAQA
                     HNLRETRQDPTAHAEVIVIQDAARHMGSWRLIDTTLYVTLEPCTMCIGAIVLARIARL
                     VFAATDPKAGACGSIMNIPPEPRLNHRVEVVGGVCAEESQTLLQDFFRQLRKDAARRE
                     IT"
     gene            complement(2005..3021)
                     /locus_tag="PKK81_11235"
     CDS             complement(2005..3021)
                     /locus_tag="PKK81_11235"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNV33501.1"
                     /translation="MTHEELEDAVPLYAIGALERSERQAIEAHLLSGCAACHAALKDY
                     QTVASLLPFGLTPATPPNTLKAKIMMAPTPIAGAVEAEQAGPKSSLEPGEWMNHLFPP
                     IAPARSLPFRLAMGFAAAVLIVGGGYLAWSSYTQTSQRSGEIQQLHAAVQQETARVAT
                     LQSELQQREQTIGTLKSEIEQRTTEVAELRDQILQREAELDDAHTQLTQNESSLQRLA
                     RQNEEFAGLFKNPSSKVVSLAGSDMAKSAGAFLLFDPSTKKAWLYAYNLPALPSGKVY
                     QLWAIDDKPVSAGVFGLDAGLKARMLIRNMTEFSRMKKFAVTVEPDGGRPEPTGAIYL
                     IGQI"
     gene            complement(3018..>3649)
                     /locus_tag="PKK81_11240"
     CDS             complement(3018..>3649)
                     /locus_tag="PKK81_11240"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV33502.1"
                     /translation="CARAQADCMEAASRQAASTIDPKLLARVAKGDQQAFGQLYDQSS
                     TLLFTLAYRILNDRDEAAELLQEVYLEVWRKIAKYDVGRGSPIAWLVTLTRSRAIDRL
                     RARASRGQHVVADSFEHPLVSRTPDVSPNPYEAREDTELRQLMAKAILELPLPQQQAI
                     EMAFYQGLTHTEIAAKLNQPLGTVKTRIKLAMAKLRASLQQTLPQGEGV"
     assembly_gap    3650..3749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3750..4687)
                     /locus_tag="PKK81_11245"
     CDS             complement(<3750..4687)
                     /locus_tag="PKK81_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33503.1"
                     /translation="MPLIWCSISGHGYGHAAQVVPVLNALGRLVPDLSAILRTTVPAG
                     FFEPRLDIPWQLKFGQQDIGCIQDGPLKIDVSRTWAAHSVLHSDWQQKIEAEAALIRS
                     AGPACVLSDISHLGIAAGAATGIPTIGLCSLSWDLVLEPFADPARREHATILRQIREA
                     YAKADCFLRVAPALAITAFHPVREIGPIAEPAVPQPAALRKAIGAGQSERVVLVGFGG
                     IALRAFPFEHMEAMDGFRFVVDGAVPDGLTRVLSLVSLGMTFKAVMASVDMILTKPGY
                     GTIVEAVALGIPVVYVRRYNFPDEPPLVDHLHRYGRG"
     gene            complement(4846..5046)
                     /locus_tag="PKK81_11250"
     CDS             complement(4846..5046)
                     /locus_tag="PKK81_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33504.1"
                     /translation="MTLDAARQRIESAIAQYGQHAAMAIELVINEVRSDMGRDAANEL
                     IDEFDLELMYNIAPMESDYSNS"
BASE COUNT          919 a         1495 c         1624 g         1042 t
ORIGIN      
        1 aacggcagcg ggaagtcgac ctttgtgaaa gtgctcaccg gactgtacct tccgcagcag
       61 ggggaggtca ggctgaacgg cgaggcgatc acgcccgcga cccaggactg gtaccggcag
      121 cattttgcgg cggtgttttc cgatttctac ctgttcaaga aactgttggg gctcgatccg
      181 gcactgattg agagtcaggc ggacggctgg ttgaagacac tccggattca acacaaggtc
      241 acgatccgcg acggcgagta ttcgacgatc aacctgtcac agggccaacg gaagcgcctc
      301 gctctcgtga cggccatgct ggaagatcga ccgttttatg tgttcgacga atgggcggcc
      361 gatcaggatc cacagtacaa ggaagttttt tacggtgagc tcttgccgga gttgcgggcg
      421 cgaggaaaag gtgtcatcgt ggtcacccat gacgaccggt atttccatgt gggcgaccgc
      481 gtgctaaaac tggacgaagg caagatcctg gaaattccga actcgactgc ttcaaccggc
      541 tcgcaccgag cgaccccggt gttcaagccg gtgatccgct cctcggcgta acgcagggtc
      601 tgcgcctcgt gtatctcaac ccatgtccgg attgctgatt ccctccagag attcttcgcc
      661 gtcgcccgct ctgagccggg aggatgtgca tgtctggttc tgccgtcttg cccggcatga
      721 cggccgacgc gccgcccttg ccgccctgct ctcgcgtgat gaacagaccc gcgcggcccg
      781 gtttgccttc gagcgggatc gtcaccggtt tatcctgtcg cacggtttgc tgcgcgccat
      841 tctggcccgc tacgtcggca gagaagccac gcagattgag tttgcaaccg gagcccatgg
      901 caagccggct ctgagcggtc agtcctgtgc cgtgcaggat atccaattca gcctttccca
      961 ttcaggggcg tatgccgtgg tggcggtggc agccggccgg gccgtcggcg tggatgtaga
     1021 ggtgcgcagg ccggacgtgg atgcgctcaa gctcgcggag cggttttttg cggcagggga
     1081 agcgcagcag attattcagg cacaggggga cgcgcaacag cgattgttct atcgctcttg
     1141 gacggcgaag gaggcctatc tcaaagggcg tggggtgggg ttgtctctcg ggctggatcg
     1201 attcgagatc ctattcgatg acgggtcgcc gctcgctcat gtgcggatga ccgactccgg
     1261 agccctcgat acgaactggc aggtgcagtc cctctcattg gccgatgacg tctccggggc
     1321 gctcgccgtt gaaggccaag cctggcaggt tcgaatgtgt gattcgagta cagatctgtt
     1381 tcgttaggtg atctcccgcc gggctgcgtc ttttctcagc tgccgaaaga agtcctgtaa
     1441 caacgtctga ctctcttcag cacagactcc tccgacgacc tccacgcggt ggttcagtcg
     1501 cggctcaggg ggaatattca tgatggatcc gcaggcgccg gctttgggat cggtggccgc
     1561 gaagaccagc cgcgctatgc gagcgagcac gatggcgccg atgcacatcg tgcagggttc
     1621 cagcgtgaca tagagggtcg tgtcgatgag tcgccagctc cccatatgcc gggctgcgtc
     1681 ttgaatcacg atcacttccg catgagccgt cgggtcctgc ctggtttccc tgagattgtg
     1741 ggcttgagca atcacgatgc cctcatgtac caataacgcg gcgatcggca cttcaccgat
     1801 cagtggcgcc gaacgcgcga gtgtcagggc ttgttgcatg aagcgggtat cgagatcgtc
     1861 gagggggagc atcgtgtcgt cggatgaatc ctgtcagggg ctgaacgcta cggcagtcga
     1921 tagagcgcct gcatgctagc acgttctgtg tgtggaatgg caccgggaga ggaggcgtga
     1981 gcggaaggtc gagtcaccgg ccggtcagat ctgcccgatc aaataaatcg cgcccgtggg
     2041 ttccgggcgt ccgccgtccg gttccacggt cacggcgaat ttcttcatcc gcgaaaactc
     2101 cgtcatattc ctgatgagca tacgagcttt cagtcccgca tccagtccga agacgcccgc
     2161 gctcaccggt ttgtcgtcga tggcccagag ctggtacacc ttgccgctcg gtaaggccgg
     2221 cagattgtac gcatagagcc aggctttctt ggttgacggg tcgaatagca ggaaggcgcc
     2281 tgccgatttg gccatgtcgg acccggccag cgacacgact tttgacgagg ggttcttgaa
     2341 cagtccggca aattcctcat tctgcctggc caggcgttga agggaggatt cgttctgggt
     2401 cagctgggtg tgcgcatcgt ccaactccgc ttcccgctgc agtatttggt cgcgaagttc
     2461 cgcgacctcc gtggtccgtt gctcgatctc cgacttgagc gtgccgatgg tctgttcacg
     2521 ctgttgcagc tccgactgca gggtggccac acgggcggtt tcctgctgca cggcggcgtg
     2581 cagttgttga atctcgcccg aacgctgaga ggtttgcgtg taggacgacc acgcgagata
     2641 cccgcctccc acaatcagga cggccgcggc aaatcccatt gccagacgga acggcagcga
     2701 gcgggccggc gcgatgggcg ggaacaggtg gttcatccat tcgcccggtt ccaggctcga
     2761 cttcgggccg gcctgttccg cttccaccgc acccgcgatc ggtgtcggcg ccatcatgat
     2821 tttggctttg agcgtattcg ggggtgtcgc cggggtgagc ccgaacggca gcagggacgc
     2881 cacggtttga tagtccttga gagcggcatg gcaggccgcg catcccgata acaggtgggc
     2941 ttcgatggcc tgtcgctccg agcgttcgag ggcgccgatc gcgtaaaggg ggaccgcgtc
     3001 ttccagttcc tcgtgtgtca taccccttca ccctgcggca atgtctgctg caacgatgcg
     3061 cggagcttgg ccattgccag tttgatgcgc gtcttcaccg tccccagcgg ctggttcagc
     3121 ttggcggcaa tctccgtatg agtcaggccc tggtaaaacg ccatctcgat ggcctgttgt
     3181 tgcggcagcg gcaactccag aatggctttc gccatcagct ggcgcagctc ggtatcctcc
     3241 cgggcttcgt acggattcgg gctgacgtcg ggtgtgcgcg aaaccagggg atgttcgaac
     3301 gaatcggcga cgacatgttg cccgcgcgat gctcgtgcac ggagccgatc gatggctcga
     3361 ctgcgggtga gtgtcaccag ccaggcgatc gggcttccac ggccgacatc gtatttggcg
     3421 attttgcgcc atacctccag gtagacctcc tgaagtaact ctgcggcctc atcgcggtca
     3481 ttcaagatgc ggtacgcgag ggtaaagagc agggtgctcg attgatcgta cagttggccg
     3541 aatgcctgtt gatcgccctt ggccacacgg gccaggagtt tggggtcgat cgtcgatgca
     3601 gcttgtcggg aggcagcttc catgcagtcg gcctgggcgc gggcgcaatn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgcgaccata tcgatgcagg tgatcgacca
     3781 ggggcggctc gtcggggaaa ttgtatctcc gcacatagac gacggggatg ccgagcgcca
     3841 cggcttcgac gatcgtgccg taccctggct ttgtgaggat catatctacg gacgccatga
     3901 ccgctttaaa tgtcatgccc agggagacca gcgacaggac gcgagtcagt ccatccggga
     3961 cagcgccatc cacaacgaag cgaaagccgt ccatcgcctc catgtgttcg aagggaaacg
     4021 ctcgcagggc gatgccgccg aacccgacca gcacgacccg ttccgactgc ccggcgccga
     4081 tggccttacg gagcgccgcg ggttgaggga cggccggttc cgcgatgggg ccgatttcgc
     4141 gaacaggatg aaacgcggtg atcgcaagag ccggcgccac acgcaggaag cagtcggctt
     4201 tggcataggc ctcgcggatc tggcgaagaa tcgtcgcatg ttcgcgccgg gcgggatctg
     4261 cgaacggctc caggaccaga tcccaggaca aggagcagag gccgatggtc ggaatgcccg
     4321 tagccgcgcc ggcggcaatg ccgagatggg agatatcgga aaggacacag gcgggtccgg
     4381 cactgcggat gagcgcggct tcagcctcaa ttttctgctg ccaatcgctg tgcagcacag
     4441 agtgggcggc ccaggtgcga gagacatcga tcttcagggg gccgtcctgg atgcagccga
     4501 tatcctgctg cccgaacttg agttgccagg gaatgtcgag gcgaggttcg aagaagccgg
     4561 cggggactgt tgtccgcaaa atggcgctga gatccggcac caatcgtccc aacgcgttga
     4621 gcaccggcac tacttgcgcc gcgtgcccgt agccgtggcc tgagatggaa caccagatca
     4681 gcggcatggc aggctgctga aaaagacccc caacgtcgtt ctcgactcgg aatcctcctc
     4741 aacgtacctc tgaacgtacg tctccgggtc ttccgcctct gcggcctcgt tggatggtct
     4801 ttttgagcac catgcgaggg ctgtgcgtaa catccccatc cgggctcagc tgttcgagta
     4861 gtcggattcc atcggggcga tgttgtacat cagttcgagg tcgaactcgt cgatcaattc
     4921 gttcgcggcg tcccgtccca tgtcggaccg tacttcattg atgaccagtt cgatcgccat
     4981 ggccgcatgc tggccgtatt gtgcgatcgc agattcgatc cgttgtctgg ctgcgtcaag
     5041 agtcatacat cctccaaccg tcctacccga gcgtcctcaa gagttgttgc agttccggca
     5101 ccagatccac accgccgttg gatcgcccgg agacggctgc gtcgatcccg gccttcaaga
     5161 caggtttggc ctcttccttc
//
LOCUS       DAOQRM010000166         5171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16725_length_5171_cov_16.412627, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000166 DAOQRM010000000
VERSION     DAOQRM010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16725_length_5171_cov_16.412627"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..830
                     /locus_tag="PKK81_11255"
     CDS             252..830
                     /locus_tag="PKK81_11255"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV33505.1"
                     /translation="MGGKLAFVATCLILVSGLAKTGLAAELPGIPPEVVADYLHAVIE
                     ADRTFYTIHVVERMQKQGGTPASETWRKDKTTLPLPAQFLREASELATLTGTRVRYRL
                     MSLWPINPQNAPASNSERNSLEAVRLHPERSVSGTVTIGSQSYFQAVYADRAVTQACI
                     ACHNAHPQSPKHDFKIDDVMGGMVIEIPLSKE"
     gene            918..2342
                     /locus_tag="PKK81_11260"
     CDS             918..2342
                     /locus_tag="PKK81_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNV33506.1"
                     /translation="MKFSTYDPGEFYDELFEGIGRPRRGSALLLRKFASLPDGELRKR
                     QQAAERVILNMGMTFGVYGSDEGHEQIFPFDIVPRIVEAPDWTLIDTGLRQRLRALNL
                     FIDDVYHEQKILKDNVIPSELIYSSKGFLKACWGLNPPRGIWCHIAGIDLVRISDGRY
                     YVLEDNMRCPSGVAYVLEARQVMKRTFPELFEAYRVRPVDEYPSRLLETLRSLSDLPD
                     PTIVILTPGSYNSAYYEHSLLAQKMGVELVEASDLVVIDGSVHMRTTKGSQRVDVIYR
                     RINDEYLDPLVFRPDSLLGVPGVMEAYKNGRVAIANAPGTGVADDKAIYAYVPKIINY
                     YLAEEPILPNVPTYVCWERRDRDYVLEHLDQLVVKATNEAGGYGMMIGPQASQQERED
                     CARRIQADPRNYIAQPTLALSRSPTLVEDHIEGRHVDLRPYVLQGKGFYVLPGGMTRV
                     ALRKGSLVVNSSQGGGNKDTWVLS"
     gene            2416..3396
                     /locus_tag="PKK81_11265"
     CDS             2416..3396
                     /locus_tag="PKK81_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNV33507.1"
                     /translation="MLSRVASSIYWVNRYIERAENYARFIEVNLNLSLDLPRGTVEQW
                     EPLVATTGDHEMFVARYGKATKETVLHFLVADAENQNSILSCLLGARENARSIKEIIP
                     SEMWEQVNRFYLMVRDAVTRGMSHHDLHAFLADVKAASHLLWGITDATMSHGEGWHFA
                     RLGRLLERADKTSRILDVKYFILLPTADEVGTPFDIIQWSDLLKSASALEMYYKRYGR
                     IAPDDVAAFLILEPTFPRAIRYCLIKAEDSLHAISGSERGSYQNLAEKRLGRLRAELD
                     FVDIEDCVTRGLHEFVDHFQARLNQVGEAISETFFVPRRVHYTESVTEEQ"
     gene            3411..4163
                     /locus_tag="PKK81_11270"
     CDS             3411..4163
                     /locus_tag="PKK81_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33508.1"
                     /translation="MKVEEGLVGIADTRVTTGAECITAGKVSIHQHGRHSMFLMTSGL
                     RSVRDKAVTYFDEAISESDQTFDKLFKAVNVFAAQIRRVAEEDKSALYNAGLTFNLHA
                     LVGGQLENDLTHKLYLIYPQGNWVEVSEGTPYCIIGESGYGKPLLDRVLRYNSSMDLA
                     LKVGFLAFDATRTSSTSVEYPLDVVLYRHDTFDILEHRFQKEDLAEISSWWQSRIADS
                     VEKLPASWVDRLLEALPKGTRSSPQDHGNSAS"
     gene            4967..>5171
                     /locus_tag="PKK81_11275"
     CDS             4967..>5171
                     /locus_tag="PKK81_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV33509.1"
                     /translation="MTSSQKLLRVTTLLGFALVCATVLWPSHALPENAPVTLPIETVA
                     NYLHAVIEADRDVYTRHVVERLQT"
BASE COUNT         1139 a         1526 c         1445 g         1061 t
ORIGIN      
        1 cccagcccgc agtaatcatc cgctgcaagg ccgcgcgcga gagcttcggc tcgcggtgca
       61 cgaggaatac gtccaaccgt ttgggctgct ccccggcaga gatgacaaat tccgtttgca
      121 taacgctgtt acgctacggc agcgcggcaa ccgaatgcaa tcgtagaagg ccgaccgatt
      181 gcctctcgac gacaatcggc ctatgctgag cgttgcgagg ccggttcgac ttatgagaag
      241 gagggtggac catgggaggg aagctcgcgt tcgtcgcaac gtgcctgatc ctggtgagcg
      301 gactcgcgaa aacaggacta gcagcagagc tgccgggaat cccccccgaa gtagtcgctg
      361 actacctaca tgcagtgatt gaagcggacc gaacgtttta caccatccat gttgtcgagc
      421 gaatgcagaa acagggcggt acacccgcat cggaaacctg gcggaaggac aagaccacgt
      481 tgccgctgcc ggcccaattt ctgcgggagg ccagtgaatt ggcaaccttg accggaacca
      541 gggtgcgcta ccgcctcatg agcttgtggc cgatcaaccc tcagaacgca ccggcgagca
      601 actcggagcg aaacagcctg gaagccgtgc gactccaccc cgaacggtct gtttccggca
      661 ccgtcaccat cggtagtcag tcttatttcc aagccgtcta tgccgaccgc gccgtaacac
      721 aggcctgcat cgcttgccac aatgcgcacc cgcaaagccc gaaacacgat ttcaagatcg
      781 atgacgtcat gggcgggatg gtgatagaaa ttcccctgag caaggagtag gttcggacgg
      841 cagcgtcgat acgccgcggg gaaacggcgc tccctgccga gccgtccgaa gaacgacgga
      901 aggcactgac acctgacatg aagttttcca cctacgaccc aggcgagttc tacgacgaat
      961 tgtttgaagg gatcggccgg ccccggcggg gaagcgcgtt actgctgcgg aagtttgcat
     1021 cgctccccga tggagaactt agaaagcgcc agcaagcagc ggaacgcgtc atcctgaaca
     1081 tgggcatgac gttcggcgtg tacggcagcg acgaggggca cgagcagatc tttcccttcg
     1141 acatcgtacc gcggattgtg gaggcaccgg attggacgct catcgacact ggccttcgcc
     1201 aacgcctgcg cgcactaaac ctatttatcg atgatgtgta tcacgagcaa aagatcctca
     1261 aagataacgt catcccgagc gaactgatct attccagcaa agggtttctg aaagcctgct
     1321 gggggctcaa tccccctcgc ggcatctggt gtcacatcgc cgggatcgac ctggtgcgca
     1381 tcagcgacgg ccgctattat gtccttgagg ataatatgcg ctgcccgtcc ggggtggcgt
     1441 atgtgctgga agccagacag gtgatgaaac gaacttttcc agagctgttt gaggcctatc
     1501 gcgtcagacc ggtcgatgaa tacccaagcc gcctgctgga aacccttcgg tccttgtcgg
     1561 acctccccga tccgacgatc gtgatcctca cgcccggcag ctacaattcc gcctactacg
     1621 aacattcact tctggctcaa aaaatgggtg tggaactggt ggaagccagt gacctggtcg
     1681 tcatcgatgg ctcggtccac atgcgcacca cgaagggctc tcaacgggtg gacgtgatct
     1741 atcggcggat caatgacgaa tatttggacc cgttggtgtt ccgtcccgat tctctgctgg
     1801 gagttcccgg tgtcatggag gcctataaga acggccgcgt ggcgatcgcg aatgcgcccg
     1861 gcaccggcgt cgccgatgat aaggcgatct atgcctacgt ccccaagatc atcaactact
     1921 acttggcaga ggaaccgata ctccccaacg tgcccaccta cgtctgctgg gaacgccggg
     1981 atcgagacta cgtgctggaa catctcgatc agttggtagt caaggcgacg aacgaagccg
     2041 gtgggtacgg catgatgatc ggcccccagg cctctcagca agaacgagaa gactgtgccc
     2101 gtcgcatcca ggccgaccct cgcaactaca tcgctcagcc cacgctcgcg ctctcccgat
     2161 cgccgactct ggtggaagac catatcgagg gacgacatgt cgatcttcgt ccgtacgtct
     2221 tgcaggggaa agggttctat gtgttgccgg gcggcatgac tcgcgtggcc ttgagaaaag
     2281 ggtccttggt cgtcaattcc tcccaaggtg gcggcaacaa agatacgtgg gtgctgtcat
     2341 gagtcttgcg cgcgcacacg cctcctgccg atcggtcggc cggcggctct gcgaacacct
     2401 tggtcggaga ccgcgatgct gagcagagtg gccagttcca tctattgggt gaaccgctac
     2461 atcgaacggg cggaaaacta tgcccggttc atcgaggtca atttgaatct ctctcttgac
     2521 cttcctcgcg gcacggtcga gcagtgggag ccgctggtcg cgacgacggg cgatcatgag
     2581 atgtttgtcg cgcgctatgg aaaagcgacg aaggagacgg tgcttcattt ccttgtggcg
     2641 gatgccgaga accagaattc cattctgtcc tgtctcctgg gcgcgcggga aaacgcacgg
     2701 tcgatcaagg aaatcatccc cagtgaaatg tgggaacagg tcaaccgctt ttacctgatg
     2761 gtcagggacg ccgtcactcg cgggatgtcg caccatgacc tccacgcatt tttggccgac
     2821 gtcaaagccg cgagccatct actgtgggga atcaccgatg ccaccatgtc ccatggagag
     2881 ggttggcact ttgcacggct cggccgcctc ctcgaacgag cggacaaaac ctctcgcatt
     2941 ctcgatgtca aatatttcat cctcttgccg accgccgatg aagtcggcac gccgttcgac
     3001 atcatccaat ggtcggattt attgaaatcc gcgagcgcct tagagatgta ttacaaacgg
     3061 tatggacgca tcgctccgga tgacgtggcc gcctttctca tccttgaacc caccttcccg
     3121 cgggcaatcc gctattgcct cattaaagcg gaggactccc tgcacgcgat tagcggatcc
     3181 gaacggggat cctatcaaaa cttggccgaa aaacgattgg gacgcctgcg cgccgaacta
     3241 gactttgtcg acatcgaaga ctgcgtcaca aggggtctgc atgaatttgt ggaccacttt
     3301 caagcacgac tcaaccaagt aggcgaggct atttctgaaa cgttcttcgt gcctcgccga
     3361 gttcactaca cagagtccgt cacggaggag caatgacgtt ttgcttggga atgaaggtgg
     3421 aggaggggct ggtcgggatc gccgacacgc gggtgacgac aggggcggaa tgcatcaccg
     3481 cgggaaaggt atccatccat caacatggcc gccactcgat gtttctcatg acatcaggat
     3541 tacgatcggt tcgagacaaa gctgtgacct atttcgatga ggccatcagc gagagcgacc
     3601 aaacgttcga caaactgttc aaagcggtca acgtgtttgc cgcacagatc cgccgtgtgg
     3661 ccgaggaaga taaaagcgcc ttatataatg ctggactgac cttcaatctc cacgccctgg
     3721 tgggcgggca actggaaaac gacctcaccc acaaacttta tctgatctac cctcaaggca
     3781 attgggtcga agtcagtgaa ggcaccccct actgtatcat cggcgaatcc ggctacggaa
     3841 agccgctcct cgatcgcgtg ctgaggtata actccagcat ggacctcgcg ctgaaggtcg
     3901 gcttcctcgc cttcgatgct acacggacga gctccaccag cgtcgaatat ccgctcgatg
     3961 tcgtgctgta tcggcacgat acgttcgaca ttctcgaaca ccgctttcaa aaagaggacc
     4021 tagctgagat ttcttcttgg tggcaatcgc gcattgccga ttcagtggaa aagcttcccg
     4081 cctcgtgggt agatcgtttg ctggaggctc tgccgaaagg cactcgctct tcccctcagg
     4141 accacggcaa ttcagcatcg taagtccggc tcgcgattct ccgctagccc ggccaacgac
     4201 cgacaaactg tgtcccgatg acacgtcacg atcaatcgcg ccgtcgacca agctcgatgc
     4261 actggccgcg ccacatatct cttcccagtc caaaggtcga tatcaccggg aaggatcgcc
     4321 actgcgtttc aatcgaagaa tttccgcttc cggctcagcc atctgcagtt tcaatcgctt
     4381 gatcttttct tggagtcgac cgagcacacg gtcgtgatcg agtccccact gaccagcgaa
     4441 tggcgtacgg tttgacgcga cctcgccgct cgcccacgtt gccctgatct cgtgcgcacc
     4501 gaaggtcgta cttcacatca tggcccgagc cattgcgata tatgcgacaa gatgacgggt
     4561 aaagaaatgg acgtatttcc cctagtggcc accgatggtg tcgtgccctc ccataagatg
     4621 attagacagc gtatagatcg aagcgtaata tgatgagtga ccgtatgatc tggtgggcac
     4681 gttggttcgt acgggcaagg agctggggcg atgccttcgg taccttccca cgaccggtcg
     4741 ttttttcagg aggccatcga tagggccggc atcccggctc atcgctccgc cccgtcctcc
     4801 gcgtcgatac ctctcaactc attatcctca ttcacccggc gatcgatccc gttcgtcctc
     4861 tccacttcac caagcactca ccggccagac cgtatggtgc aattcatctg ctcacgtggt
     4921 gcgccggcca gaccggatca cccgtgtgcg caacaaggag gttggtatga catcctctca
     4981 aaaattgctg cgggtcacga ccttgctggg attcgcattg gtctgcgcga ctgtgttgtg
     5041 gcccagtcac gcactgcctg agaatgcacc ggtcaccctg ccgatcgaaa cggtggctaa
     5101 ctatctacac gccgtgattg aagcagaccg ggatgtgtat accagacatg tcgtcgagcg
     5161 cttgcaaaca a
//
LOCUS       DAOQRM010000167         5165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16750_length_5165_cov_13.572016, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000167 DAOQRM010000000
VERSION     DAOQRM010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16750_length_5165_cov_13.572016"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..529
                     /locus_tag="PKK81_11280"
     CDS             164..529
                     /locus_tag="PKK81_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV33510.1"
                     /translation="MATIMVIDDEPSIRGLLREVLERSGHKVVEAKDGREALDLYQKH
                     KADLLIMDLLMPEVDGLEATLQLTREYMDTKIIAMTGAQGDRNFLDIAKLFGAHRTFE
                     KPFDLKEMLNAVEAELAQK"
     gene            704..1102
                     /locus_tag="PKK81_11285"
     CDS             704..1102
                     /locus_tag="PKK81_11285"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNV33511.1"
                     /translation="MLIVLVGILPLHNFVGHAHWQYIRWVPTPDQLSSPAILLDLGID
                     AVANILLFLPFGLLYTLRGLGGKSFSPGHLVLMAFALSFSIEYYQVYCHNRSTSLLDL
                     LDNVLGAYIGMKAGEAYVRRYMARVVEPAA"
     gene            complement(1105..>1342)
                     /locus_tag="PKK81_11290"
     CDS             complement(1105..>1342)
                     /locus_tag="PKK81_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HNV33512.1"
                     /translation="TAQVATGTVAGGAIGSAAGAVGGAILGHPGRGAMVGAASGATAG
                     FLRGLFRRSPPSGAFTNYVDRCLRDRGYDPTGWQ"
     assembly_gap    1343..1442
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1443..2300)
                     /locus_tag="PKK81_11295"
     CDS             complement(1443..2300)
                     /locus_tag="PKK81_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33513.1"
                     /translation="MFKIKKKIEKPKPPILYNVIEDYSEFDAPGNVTACAMTLPEDQA
                     AHAKILAESYDVPLEQMTQLLAEGGVYVYPQVGGLLTRGLFACRIDATGATPKQTWVL
                     DLMQFAEAEQWARLRAVPLIEAATEVFYRTLPEELKTKLAKKNLGLDYRTLDRAVAKG
                     GDIKFIDFRKDWSPHFKRLCIMPDGRLVETGGLEEFAQLHQITVSQAKKLVEEGGTMD
                     VAGGEVLACQIVNGQPAVARFSAKNYTKAKELAASKGLHILDALSEVAYNDSVMMRTL
                     QRKDLGGRI"
     gene            complement(2360..4123)
                     /locus_tag="PKK81_11300"
     CDS             complement(2360..4123)
                     /locus_tag="PKK81_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNV33514.1"
                     /translation="MTPEQPSSAISTELLSTDPVDRVIAYHVRTKHHFNRYARSLGYL
                     DWANQPNPFRRFDGTQLVRLPLLKADEEPRSPSYDAIYQPGSVPVQPLTNRTVSRFFE
                     LALGLSAWKKAGESEWALRNNPSSGNLHPTEGYVLLPSAEGLDLKPGLYHYAPREHGV
                     ELRADCSPAAISRLLAPFPSGAFLFGLTSVHWREAWKYGERAFRYCNHDVGHAIGSAR
                     IAAATLGWRMALLDGADQNQTARVLGTHRVDDFSGVEPEHPDCLAVIWPVEAEARASS
                     SSRENQNLPLFLEEAAVTGVAVGPWHGKANELSREHGVHWDVIDQVAEASWKTSLEHP
                     IVSLAGTPIVPPGTLHASRTTDDAAAIIRQRRSAVSFDGRTSISAATFFHILQRVMPR
                     VERPQLQRPMPWDVLPWDPAIHLMLFVHRVEGLEAGLYMLARDPNKLPLLQQSMNPEL
                     EWTSAPGCPEDLPLFWLLQGNAQRLAAQVSCQQGIAGDSAFSLGMLAEFEGRLRQGGA
                     WWYPRLFWETGLLGQVLYLEAEAAGVRGTGIGCFFDDPVHEVVGIKDLSIQSLYHFTV
                     GGPVDDQRLMTLPPYHHLRHE"
     gene            4511..>5165
                     /locus_tag="PKK81_11305"
     CDS             4511..>5165
                     /locus_tag="PKK81_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33515.1"
                     /translation="MTTLNFATAAWNLLAFIEDVFLKHQLTGEENAIHQAAVLADTFI
                     AGLSYPLYWLSSNCPAGGDPSCHCDNSNYKSAWELSDLGRNYKHFCAAFTYASRGMID
                     LNIEDSRLIPVCDFLEQSEYEAYNRLIRPCLSDVPILAIGELAELIQLSLRVNNNGFS
                     YNLSPKLVKAAKQVIGPELKRRFLLPDHWQFSRYTLGDFRRVFEAITAMALIHWYARY
                     "
BASE COUNT         1093 a         1574 c         1337 g         1061 t
ORIGIN      
        1 ggagagggtc gaatcgatgc ccagctgttt cagactcttt cctggacaga ctttcgtcac
       61 cgcggtaagc ttcctctacg agtcgaattg tctgcaaccg agcccgaata ctgcgaaatc
      121 gttccatcac acgcagaacc ggtgcccatc ctgaggagta gttatggcga ccatcatggt
      181 gatcgacgac gaaccatcca tccgcggcct cctgcgggag gtcctggaac gatcagggca
      241 caaggtcgtc gaggcgaaag atgggcggga ggcgctggat ctctatcaaa aacataaagc
      301 cgacctgctg atcatggacc tgctcatgcc ggaggtcgat ggactggaag ccaccctgca
      361 actgacccgc gaatacatgg acacgaaaat catcgcgatg accggcgcgc aaggggaccg
      421 gaactttctg gatatcgcga aactcttcgg cgcccaccgg accttcgaaa aacccttcga
      481 cttgaaggaa atgctgaacg ccgtggaagc ggaactggct cagaagtaat ccgcactccc
      541 ctccgcgccc ctgcccttcc cccggaagcc gttcacgaag gaaaatctgc tggaaacccc
      601 accgctgctt ctgcaagaat cgaacggtgc agcggacagc cacagccaaa cacccgtgtt
      661 tctaaccccc actcgcgcat ggcgtctctg gtgtgcctgg agcatgctga tcgtgctcgt
      721 gggtatcctc ccgctgcata actttgtcgg ccacgcgcat tggcaataca ttcgctgggt
      781 gccgacaccg gaccagctga gttcgccggc catcctcctg gatctcggca tcgatgccgt
      841 ggccaacatc ctcctcttcc tccccttcgg cctgctttat accctgcgcg gattgggcgg
      901 caaatccttc tctcccggcc accttgtgct gatggccttc gcactgtcct tcagcatcga
      961 gtactaccag gtctattgtc acaaccgcag cacatcgctt ctcgacctgc tggacaatgt
     1021 tctgggtgcg tatatcggga tgaaagcggg ggaagcgtac gtcaggagat atatggcacg
     1081 agtggtggaa ccggccgcat aagattattg ccagcctgtc ggatcatatc cccgatcacg
     1141 cagacaacga tccacatagt tggtaaacgc cccgctcggc ggcgatcgcc tgaacaggcc
     1201 tcgcagaaac ccggccgtcg cgccgctggc tgcgccgacc atggctccac gccccggatg
     1261 tcccaggatc gctccgccca ccgcaccggc ggccgatccg atcgccccgc ccgccaccgt
     1321 gccggtggca acttgcgcgg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnttaaatcc gtccccccag atccttccgc tgcaacgttc gcatcatcac cgaatcgtta
     1501 taggccactt cgctcaaggc atcgagaata tgcagccctt tgctcgccgc caattctttc
     1561 gcttttgtgt agttcttcgc gctgaagcgg gcgactgccg gttgaccgtt cacgatctga
     1621 caggccagca cttctccgcc ggccacatcc atcgtcccac cctcctccac gagtttcttg
     1681 gcctgcgaca ccgtaatctg gtgcagctgc gcaaactcct ccagcccgcc cgtttccacc
     1741 aaacgtccat cgggcatgat gcagagccgc ttgaaatggg gagaccagtc tttgcggaag
     1801 tcgatgaact taatgtcgcc gcccttcgct accgcacggt cgagggtccg gtaatcgagg
     1861 ccgagattct tttttgccag cttggtcttt aattcctcgg gaagcgtccg atagaacacc
     1921 tctgtcgccg cctcgatcaa cggcaccgcg cgcaaccggg cccattgctc cgcctcggca
     1981 aactgcatca ggtcgagcac ccaggtctgt ttcggcgtgg cgcccgtggc atcgatccgg
     2041 caagcaaaga ggccacgcgt cagtaatccg cccacttggg gatatacata gacgccgccc
     2101 tccgccagca gctgcgtcat ctgctccaac ggcacgtcgt aactttcggc cagaattttc
     2161 gcatgcgctg cctggtcttc cggcaacgtc atggcgcagg ccgtcacgtt acccggcgca
     2221 tcgaactccg agtaatcttc gatcacgttg tacaaaatcg gcggcttcgg cttttcaatc
     2281 ttcttcttga tcttaaacat aatgagctcg ttcctcgtat ctcgtcgttc gtctctcgtt
     2341 caaagcaggt cctgcgcaat cactcatgcc ggaggtgatg atagggagga agcgtcatga
     2401 gccgctggtc gtccaccggc ccgccgactg taaagtgata cagcgattga atggacaggt
     2461 ccttgatgcc cacgacctca tgcaccgggt cgtcgaaaaa gcaaccgatg ccggtgcccc
     2521 gcactcccgc tgcctccgct tccagataga gcacctgccc gagcaggccc gtttcccaga
     2581 acaggcgtgg ataccaccaa gccccgccct gccgcaagcg gccttcgaac tccgccagca
     2641 tcccgagcga aaaggcacta tcgccggcaa tgccctgttg gcaactgact tgcgccgcca
     2701 gtcgttgtgc gttgccctgc aacagccaga acaggggaag atcttccggg cagccgggcg
     2761 ccgaggtcca ttccaactca ggattcatcg actgctgcag cagcggcagc ttgttcgggt
     2821 cacgcgccag catatacaga cccgcctcca gcccttcgac gcgatgcacg aacaacatga
     2881 gatgaatcgc cggatcccaa ggcaggacat cccacggcat gggccgctgc aattgcggtc
     2941 gctccacgcg aggcatcacc cgctgcagga tgtgaaagaa tgtggcggct gaaatagagg
     3001 tgcggccatc gaaggagacg gcgctgcggc gctgcctgat gatcgccgct gcgtcgtccg
     3061 tcgttcgtga agcgtgaagc gtcccgggtg ggacaatggg ggtcccagca agcgacacga
     3121 tcgggtgctc caggctggtc ttccaagagg cctctgccac ctgatcgatg acgtcccaat
     3181 gcaccccatg ttcgcgactc agctcattgg ccttcccatg ccacggcccc acagccacac
     3241 cggtgacggc cgcctcctcc agaaacaacg gcaaattttg attctcacgc gatgagctcg
     3301 atgcacgagc ctcggcttca acaggccaga tcaccgcgag acaatctgga tgttccggct
     3361 ccaccccgct gaaatcgtct acgcggtggg ttcccagtac cctcgcggtc tggttctgat
     3421 cggctccatc caacagcgcc atcctccaac cgagtgtcgc cgccgcaata cgcgcggacc
     3481 cgatcgcatg gccgacatcg tggttgcagt agcgaaacgc ccgctcgccg tacttccagg
     3541 cttcccgcca atggactgag gtgagaccga acagaaacgc tcctgacgga aagggagcca
     3601 atagacgcga gatggcggcc ggcgaacagt cagcgcgtaa ctccacccca tgttctctcg
     3661 gtgcgtagtg atagagcccc ggcttcaggt cgagcccctc ggccgacggc agaaggacgt
     3721 agccttccgt cgggtggaga ttgccggacg aaggattgtt ccgcagggcc cattccgact
     3781 ccccggcctt cttccaggct gagagcccga gggccagttc gaaaaaccgt gaaacggtcc
     3841 tgttggtcag cggctgcaca ggaacagacc ccggttgata gatggcgtcg taggacggcg
     3901 accgtggctc ctcgtctgcc ttcagcaatg gcaagcgcac caactgcgtc ccgtcaaatc
     3961 gccgaaaggg attgggctgg tttgcccagt ccagataccc caatgaccgc gcatatcgat
     4021 tgaaatggtg tttggttcga acgtgatagg caatcacgcg atcgaccgga tcggttgaaa
     4081 gcagctcagt ggagatggcg gaagacggtt gttcaggagt catggcggcc agtctaacga
     4141 ggcggaggaa ggaaagtaaa cgcgttggga ctagcttcag ccttggtcaa ctgacagaca
     4201 tatgtgactt atcccgaagc cctcggaatc tatgacgtcc caccatctca ggccccgcaa
     4261 taattagcca ggcacaaact gctcggaatt ttatacagaa atggttaagc tccgccccat
     4321 agagttctat gctcaatggc tatgctgttc cgtctagcag atgactagct cgacgcgtga
     4381 cgcttgcctg cagatactct gaccaagcgc ttgttcactt acgcctatgc cacctcgaaa
     4441 tcctgatcgt ctttccccac aactacgatc catcgaatcg gaaattgata acgcctataa
     4501 agcgaatcct ttgactacgc taaattttgc cacggccgct tggaacttat tagcatttat
     4561 tgaggatgta tttctgaaac accaactaac tggtgaggaa aatgcaattc accaagcagc
     4621 ggtgcttgcc gatacgttta tagctggtct cagttatccc ctctattggt tgagctcaaa
     4681 ctgtccagct ggcggtgatc catcatgcca ttgcgacaat agcaactata aatctgcatg
     4741 ggagctatcg gatcttggaa gaaactataa acacttctgc gctgcgttta cttacgccag
     4801 tcgcggcatg atcgatttga atatcgagga ttcaagatta attcccgtat gtgatttctt
     4861 ggaacaatct gaatatgaag cttacaaccg gttgattagg ccgtgcctct ctgatgtacc
     4921 gatactggca atcggggaac tcgctgaatt gatccagctc tcgttgaggg tcaacaacaa
     4981 cgggttctcc tacaatttaa gccctaaact ggtcaaggct gccaagcaag tgataggacc
     5041 tgaactcaaa aggcgttttc tccttcctga ccattggcag ttttcacgct acaccttagg
     5101 agattttcgc cgagtctttg aggcaatcac ggcaatggcc ctcattcact ggtatgctcg
     5161 atatt
//
LOCUS       DAOQRM010000168         5157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16792_length_5157_cov_14.540964, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000168 DAOQRM010000000
VERSION     DAOQRM010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16792_length_5157_cov_14.540964"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKK81_11310"
     CDS             <1..221
                     /locus_tag="PKK81_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33516.1"
                     /translation="FCILDEIDAPLDEENIGRFTSVLRSLSSTAQFMVITHNKRTMAM
                     ADSLFGVTMEEPGVSTLISVKLGDLQPA"
     gene            444..2621
                     /gene="shc"
                     /locus_tag="PKK81_11315"
     CDS             444..2621
                     /gene="shc"
                     /locus_tag="PKK81_11315"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HNV33517.1"
                     /translation="MRLLKNLFNRLSDSLLVSVPSRIRAARDFASAPVLRLVSNKSAV
                     PASGDSAAKRAPAHSTGQVEALEEALRKSQAWFLARQDASEGYWVAELEADTTLTSEY
                     LMLRRFLDCIDPERERKAVRYLKSAQLPDGGWSIYHGGPAEISASVKAYFALKLSGVS
                     ADEPFMVNARTCILDKGGVVAANVFTKIALALFGQYDWRGVPSMPPEIMLLPKRFYFS
                     IYAISYWSRAVLIPLLIIFAKRPLCHVPPELGIDELYTEPPAEIDYGMVPPFKKDRTW
                     FTARNFFINLDALLKIYDRSPVEWVRQKALKCAENWMLDHMKGSGGLGAIYPAMANSV
                     MALHCLGYKNDDPLMVKAIREIEELEVHDTVQDNGQCVDAMHLQPCHSPIWDTALLIN
                     ALIEAGMPQDHPALQKASTWLLSKQTKTVGDWIISAPGAEPGGWYFQFENELFPDVDD
                     SAVVLMALAKVHLSDEAQQRLAIRRGCRWVTSMQGSDGGWGAYDVDNNRIVFNYIPFA
                     DHRALLDPSTADLAGRCLEMLATLGYDWTHPAVASALTFVKNDQEQDGSWYGRWGVNY
                     IYGTWSVLSGLRAIGEDLSSPYIRRAVSWVESKQNPDGGWGESCLSYGDVSQSGRGDS
                     TPSQTAWALLALMAGGVTDSFSLARGIHYLIRNQRKDGSWEEVRHTGTGFPRVFYLRY
                     HWYCQYFPLWALAMYRNLKTRGTTRADELRLQAYQSGQFRSPR"
     gene            2676..3431
                     /locus_tag="PKK81_11320"
     CDS             2676..3431
                     /locus_tag="PKK81_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33518.1"
                     /translation="MTAIGVFVATRWESAAVCQAFAASEMRVVGGIRCAVARQGQVEW
                     WVIPMGVGPERATTTAGRVLGEQSFAAVWSTGFACALGQAVIGEVLIGTHVTMEGGAE
                     DGRSVPCAAAGADWVRQAVQQQRLPVQAGQFVSVPRVLCRAEDKQEIATRAGGVGLDM
                     ESAALGFVASERKIPFVIVRTASDLVDESLPLDFNLFLRPSGWVKGVAACLAHPTSLI
                     GLNRLRVQSRIAGTQLTAVFRACADKALREGLA"
     gene            3492..4259
                     /locus_tag="PKK81_11325"
     CDS             3492..4259
                     /locus_tag="PKK81_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV33519.1"
                     /translation="MEGIARIGRYTIDLTEQMGRMMLFVLSSFAWLTRPPFRVYQIVK
                     QLNFIGYKSTFVVVLTAVFTGMVLALQGHYTLRKFGSEAVLGSAVALSIIRELGPVLA
                     ALMVTARAGSAMTAEIGIMRITEQIDALDTMAINPLQYLIGPKLVASLIAVPLLVALF
                     DVVGIYGGYVVGVQLLNGNEGAYWSSIESAVEWKDVYGGILKSISFGLLISWVCCYKG
                     FHTRHSAEGLGTATTEAVVLSAVLILVWDYFLTSVLL"
     gene            4335..5096
                     /locus_tag="PKK81_11330"
     CDS             4335..5096
                     /locus_tag="PKK81_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV33520.1"
                     /translation="MIKLVGVEKTLGKQPVLRGVDLTIPTGKLTTIIGRSGEGKSVLL
                     KHIIGLMQPDRGEVWIDGTNIARLKGQALNEVRKKFAMLFQGAALFDSMTVFENVAFP
                     LREKLRLKGEIVTRRVEEKLEQVGLKGMGHKFPAELSGGMRKRAGLARALVMEPEIIL
                     FDEPTTGLDPLMAKAIHDLIVAMQQQFKFTAVMVSHEIPEIFGISDYVAMLKNGRIAE
                     MAPSNEFVKTTDEEIREFIFVAGTVTPKGLPTASL"
BASE COUNT          973 a         1371 c         1656 g         1157 t
ORIGIN      
        1 cgttctgcat cctggacgaa atcgatgcgc cgctcgacga agaaaatatc gggcgtttca
       61 cgagcgtgtt gcgcagcttg tcgtcgaccg ctcagttcat ggtcatcacg cacaataagc
      121 ggacgatggc gatggcggat tcgctgttcg gggtgacgat ggaagagccc ggtgtgtcga
      181 cgttgatttc agtcaagctc ggtgatctcc agccggcgta gtccgagcgg agatgagtac
      241 gtaggggctg ttccggcggg tggtgaaggc aatgccgccg cgcgaggggg gctgcttccc
      301 ttgactgcct agaaacagtt tgttatacag ttctgctaat gattataagt ggtactgtgc
      361 ggtttcatgt gatcgcttcc tgatcgatgg aaccgtctcg actatcaatc ccgccgtctt
      421 tttcagattc acggcatcac gttatgcgac ttctcaaaaa cctgttcaat cgtttgtccg
      481 atagtttgct ggtgtcggtg ccgagccgaa tcagggcggc gcgcgacttt gcctccgctc
      541 cggtgctccg actcgtctcc aataagtctg cggtgccggc ttccggcgac agtgccgcga
      601 agcgagcccc ggcacattcc acaggccagg tggaagcgct ggaagaggcg ctgcggaaaa
      661 gccaggcctg gtttctggcg cgacaagacg cgtccgaagg gtactgggtt gccgagttgg
      721 aagccgatac cacgctgaca tctgagtatc tgatgttgcg ccgcttcctg gactgcatcg
      781 atcccgagcg cgagcgcaaa gcggtccgct acttaaaatc cgctcagttg ccagacggcg
      841 gatggtcgat ttatcacggt ggcccggcgg agatcagcgc ttcggtgaag gcctactttg
      901 cgttgaagct ctccggcgtg tccgccgacg agcccttcat ggtgaatgcc cgcacctgca
      961 tcctggacaa gggtggagtg gtggcggcaa atgtcttcac gaaaatcgcc ctggccctgt
     1021 tcggtcaata tgactggcgt ggtgtcccca gcatgcctcc ggaaatcatg ctgttgccga
     1081 agcgattcta tttcagcatt tacgcgattt cctactggtc tcgtgcggtg ctgattccgc
     1141 tgttgatcat attcgccaaa cggcccttgt gtcatgttcc gccggaactg ggcatcgacg
     1201 aactctatac ggagcccccg gcagagatcg attacggcat ggttcctccg ttcaagaagg
     1261 accggacctg gttcacggcc aggaacttct tcatcaatct cgatgcgctg ctcaagatct
     1321 acgatcgttc gcccgttgag tgggttcgac agaaggcgct caagtgcgct gagaactgga
     1381 tgctcgacca catgaagggc agcggaggcc tcggcgcgat ttatcctgcc atggccaatt
     1441 ccgtgatggc gctgcattgc ctcggctaca aaaacgatga tccccttatg gtgaaggcca
     1501 tccgggagat cgaagaactg gaagtacacg acacggtgca ggacaacggc caatgcgtcg
     1561 atgcgatgca tttgcagccc tgccactccc cgatctggga cacggccttg ctcatcaatg
     1621 ccttgatcga ggccggtatg ccgcaagatc atccggcgct gcagaaggct tcgacctggt
     1681 tattgtccaa gcagactaag accgtcggcg attggattat ttcggctccc ggtgccgaac
     1741 cgggcggatg gtatttccaa ttcgagaacg aactgtttcc cgatgtggac gattccgcgg
     1801 tcgtgttgat ggccctggcg aaggtgcatc tgtccgatga agcccagcaa cgtctggcga
     1861 ttcgtcgcgg ttgccgctgg gtgacctcta tgcagggatc ggatggaggc tggggtgcct
     1921 acgacgtcga taacaatcgg attgttttca actacattcc gttcgccgac catcgggccc
     1981 tgctcgatcc cagcacggct gaccttgccg gacgctgtct ggagatgctc gcgacgctgg
     2041 gatacgactg gacccatcct gccgtggctt ctgcgctcac gtttgtgaaa aacgatcagg
     2101 agcaggacgg cagttggtat ggccgttggg gcgtgaacta catttatggc acctggtcgg
     2161 tcctgtccgg cctgcgagcg atcggtgagg atctctcgtc tccctatatc cgccgggcgg
     2221 tcagttgggt cgagtccaag caaaaccccg atggcggatg gggtgaatcc tgcctctcgt
     2281 atggagatgt ctcgcagagc ggtcgcggtg acagcacacc gtcgcaaact gcctgggcgc
     2341 tgttggcctt gatggcaggc ggagtgacgg actccttcag cctggccagg gggatccatt
     2401 acctcattcg gaatcagcgg aaggatgggt catgggaaga ggtgcgccat accggaactg
     2461 gtttcccgcg cgtgttttac cttcgttatc attggtattg ccaatacttc cccctctggg
     2521 cgctggccat gtaccgcaat ctcaagactc gtggaacgac gagggccgat gaattgcgtc
     2581 tgcaggccta tcaatcagga caattccggt cgccacgctg accgtgggtg ttcatcgagt
     2641 tcagcttttc ctgtcattgt ccctcgggag tcattgtgac cgcgatcgga gtcttcgtgg
     2701 caacccggtg ggagtcggcc gccgtgtgtc aggcgtttgc cgcgagcgag atgcgggtcg
     2761 tcggagggat tcgttgtgcc gttgcgcggc agggtcaggt cgagtggtgg gtcatcccga
     2821 tgggtgtcgg acccgagcgg gccaccacga ccgccggacg ggtgctcggg gaacagtcat
     2881 ttgccgcggt gtggtccacg gggtttgcct gtgcgttggg tcaggccgtt atcggcgagg
     2941 tgttgatcgg aactcacgtg accatggaag gtggcgcgga ggacgggcgg tccgttccct
     3001 gtgctgcggc cggagccgat tgggtgcggc aggccgtgca gcaacagcgc ctcccggtgc
     3061 aggccgggca gttcgtttcg gtgccgcggg ttctctgccg ggcggaggac aagcaggaga
     3121 ttgccactcg agcgggaggg gtcggcctcg acatggagag tgcggcgttg gggtttgtgg
     3181 cttcggagcg caagattcca tttgtcattg ttcgtacggc gtcggatctt gtcgatgaaa
     3241 gccttccgct tgacttcaat ctgtttctca ggccatctgg atgggtgaag ggtgtcgccg
     3301 cttgtctggc tcatccgacg agtctcatcg gactcaatcg acttcgtgtg caaagccgca
     3361 tcgccggaac acaattgacg gcggtattca gagcctgcgc ggataaggcg ttacgcgagg
     3421 gactggcctg accgggccca acgtgtttcg acggctgagt ttcgggtagg tggacgagag
     3481 gagagcgtgg catggaaggc attgctcgta tcggacggta tacgatcgat ctgacagagc
     3541 agatgggacg gatgatgttg ttcgtcctct cctcctttgc ctggctgacg cgtccgccgt
     3601 tccgcgttta ccaaatcgtc aagcagctga atttcatcgg ctacaagtcc acgtttgtcg
     3661 tcgtcctcac tgccgttttt accggcatgg tcctggccct gcaggggcat tacacgctcc
     3721 gaaaattcgg ttctgaagcg gtgctcggct ctgccgtggc gctcagcatc atccgggaat
     3781 tgggtcccgt tctggcggcg ttgatggtga cggcccgggc cggatccgcc atgaccgcgg
     3841 aaatcggcat catgcggatt acggaacaga tcgatgctct ggacacgatg gccatcaatc
     3901 cactgcaata tctgatcggg cctaaactcg tcgccagtct catcgccgtg ccgctgctgg
     3961 tcgcgctctt tgacgtcgtc gggatttatg ggggatacgt cgtcggcgtg caactgttga
     4021 acgggaatga aggcgcctac tggagttcga ttgagtcggc cgtcgagtgg aaggatgtct
     4081 atggaggcat tttgaaatcc atcagcttcg gcctgctgat cagttgggtc tgttgttaca
     4141 aaggctttca caccaggcat agtgccgaag ggttggggac cgcgacgacc gaagcagtgg
     4201 tgctctcggc ggtcttgatt ctggtgtggg attatttttt gacgtcggtg ctgctctaac
     4261 atcgtgaaac gtgaagcgca tgaggagaat gcggctacgg cggaacgggg cagctgccgc
     4321 ccgtacgagg ctccatgatt aaactggtcg gcgtcgaaaa gactttgggc aagcagccgg
     4381 tgttgcgggg cgtggacctg accattccca ctggaaagct gacgacgatc atcgggcgaa
     4441 gcggcgaagg caagagcgtg ctactgaagc atatcatcgg ccttatgcag ccggatcgcg
     4501 gcgaggtctg gatcgacggg acgaacattg cgcgtctcaa ggggcaggcg ctcaatgagg
     4561 tgcgcaagaa gttcgccatg ttgttccagg gtgcggccct gttcgactcg atgacggtat
     4621 ttgaaaatgt cgccttcccc ctacgagaaa agcttcgcct gaagggcgag atcgtcaccc
     4681 ggagagttga ggagaaactg gaacaggtgg gactgaaggg catgggccat aagtttcccg
     4741 ccgaactgag cggaggcatg cgcaaacgcg ccgggttggc gcgcgccctg gtcatggagc
     4801 cggagatcat tctgttcgac gaaccgacga cggggctcga tccgttgatg gcgaaagcca
     4861 ttcatgatct gatcgtggcg atgcagcaac aattcaagtt tacggctgtc atggtcagtc
     4921 acgagatccc cgagattttc gggatttccg actatgtggc gatgctcaag aacggacgga
     4981 tcgccgaaat ggcgccatcg aacgagtttg tgaagacgac ggacgaggag attcgggagt
     5041 ttatttttgt cgccgggacc gtcacgccga aggggttgcc gaccgcatcc ctctgatagg
     5101 agacgttatg gaacgcgcaa agctggaatt gatggtggga atctttgtgc tcgtcgg
//
LOCUS       DAOQRM010000169         5150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16823_length_5150_cov_14.868891, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000169 DAOQRM010000000
VERSION     DAOQRM010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16823_length_5150_cov_14.868891"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1548
                     /gene="bioA"
                     /locus_tag="PKK81_11335"
     CDS             166..1548
                     /gene="bioA"
                     /locus_tag="PKK81_11335"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNV33521.1"
                     /translation="MTRPSKRLPLAAWDHAYLWHPFTQMQEWEQEEPLIIHKGKGSYL
                     IDTEGRKYLDGTSSIWVNIHGHRHPQLDRALTTQLGQIAHSTFLGLSNPPAIRLAREL
                     IRLAPKGLRRVFYSDNGSTAVEVALKMAVQYWQQRQPSVGPKNSFLHLKLAYHGDTVG
                     AVSVGNIELFHARFKALLFPTLEVDPPYCYRCPLRLTYPACRMACIDPLESLLKARHR
                     ELAGVILEPLVQAAAGMLTAPPGYLTRVRELCTKYNVLLIADEVATGFGRTGKMFACQ
                     HEGVTPDLMAISKGMTGGYMPLAATLATEEIYRAFLGRYDEWKTFFHGHSYTGNPLGC
                     AVALANLDIFRREKTLLHVRKQTKLLTRLLRPMTLLSHVGEIRQCGLMVGIELVLDRT
                     TRTPYPLEMRIGHRVAQACRKGGLLMRPLGNVIALVPPLSTSPQELTKMVAILHSAIV
                     DVTEGSASPA"
     gene            1723..2949
                     /locus_tag="PKK81_11340"
     CDS             1723..2949
                     /locus_tag="PKK81_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33522.1"
                     /translation="MTRRLMSWGWLLLVALPLTAQAEEAQQSDLLLQRIQFTDTARES
                     GSSAIPFSIASPVLHASFQTTSFAPPTASTRLKDLVPDSERPQTKGVVAASTFFKGQL
                     TTEGEVANNATNDAGVPSRIDQRDDGTKRMVRMALTGTNGQFRYGINYRSAGKAFFNS
                     PDQTVREMWGEWGMGIAKLRSSSGQTWNNVDLDPTRARIQHTFNRIGLAVAKTAWPEL
                     SLTYTRSALASSFDPSGSIPQRSQSNSMEAALSYTGLTWNARLSSSYILTNDETRGGS
                     QSTAFAQTLVATYRPMNTLTLAPTLSYRTETQSWSGATIQTPMASLSLLYKQSQRLLV
                     SAMGGYTSTSSSDGLIDTNSIVGKGMFALHLAPIYGHPTTISLEAAYTNTTNRTVAGL
                     DTEDLSGLVRILVASL"
     gene            complement(2974..4122)
                     /locus_tag="PKK81_11345"
     CDS             complement(2974..4122)
                     /locus_tag="PKK81_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV33523.1"
                     /translation="MKQLLCQLSLILVGLTLTGCTRPDPPAPSSKTVQPPVVASRQPD
                     IQSMLIETPPSLPALTLSARVTYAEDGFSRISSPLQGPVLDVRVKLGQAVKAGDILMV
                     IDAADIATAYAAYVEEISELGLAERNYELTKDLYDAQAMSLKDLEHAENDLNRERAEF
                     KQAKGRLLSLRVPAAELSKPLAQQQITSRFELRSPLTGTVVERNVTPGQIVGPASDTP
                     LFTVANLDRLQVVADVYEHDLSGIRVGAVAPMTVEAYPGIEFPATISVIGDVVDPATR
                     TIKIRASVSNVDRRLKPEMFARVTIAGHTMRPKIVIPKQAILERSGKQWVVVQHDAGR
                     LEERAINIDSVIGNQVTIREGLTTGERILLTPSTINHLAAGDTSAGGA"
     gene            complement(4482..>5150)
                     /locus_tag="PKK81_11350"
     CDS             complement(4482..>5150)
                     /locus_tag="PKK81_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNV33524.1"
                     /translation="TPHDVADAAYLLKSCYRYFPDGTIHVAVVDPGVGGTRRPLLVSS
                     SRYVFIGPDNGLFTHIYEEEQGVEVRQIENRQYRLDSDGATFDGRDLFAPAAAWLTKG
                     QPIGSFGRLVPNYERLPIAEPAWDKHVMAGHILYIDRFGNLISNLTAYHLKEVRGLTK
                     RPEPYIRIGGITIDGMVRSYAEGSPDTPQALINSNGYVEVFLKEGRAADRLKVERGQR
                     IELC"
BASE COUNT          991 a         1531 c         1539 g         1089 t
ORIGIN      
        1 agactttgca aatcctggct catgttcccc aaaatcggtt cattatagtg accgctccca
       61 aggttgtcaa agaaatcgcc cgccgttata atccgcgcgc ggcgcgggat ttttcgtcgt
      121 cgacgcgatc gtgccgcacg agttgttctg tatcctcctg tcatcatgac tcgaccatcc
      181 aagcgccttc cacttgccgc ctgggaccat gcctatctgt ggcatccgtt tacgcagatg
      241 caggaatggg agcaggagga gcccctgatc atccacaagg gcaaaggttc ctacctcatc
      301 gatacggagg gccgaaagta tctcgacggc acgtcgtcca tctgggtcaa tattcacggt
      361 caccggcatc cgcaattgga tcgcgccctc acgacgcagc ttgggcagat cgcgcactcc
      421 acgtttctcg gcctgtccaa tccgccggcc attcgtctcg cgcgggaatt gatccgtctc
      481 gcgcccaagg gacttcgacg ggtgttttat tccgacaacg gttcgacggc ggtcgaagtg
      541 gcactgaaga tggcggtgca atactggcaa cagcggcagc cgtccgtagg acccaagaac
      601 tcctttcttc atttgaaact ggcctaccac ggggacacgg tcggtgccgt cagcgtgggg
      661 aacatcgagc tctttcatgc gcggtttaaa gccctcctgt ttccgacgct cgaggtggat
      721 ccgccctatt gttatcgttg tccgctgcgc ctgacgtatc ccgcttgtcg catggcctgc
      781 atcgatccgc tggagtcgct gttgaaggcc cgtcatcgcg aactggccgg ggtcattctc
      841 gaaccgctcg tgcaagcggc ggcggggatg ttgaccgcgc cgcccggata cctcacccgc
      901 gtgcgtgagc tgtgcacgaa gtacaacgtg ttgttgattg cggatgaagt ggcgaccggc
      961 ttcggtcgca cgggcaaaat gttcgcctgt cagcatgaag gtgtgacgcc ggatctcatg
     1021 gcgatcagca agggcatgac cggcggatac atgcccttgg cagccaccct ggcgacggag
     1081 gagatttatc gcgccttctt gggacggtat gacgaatgga aaacattctt ccatgggcat
     1141 agttacacgg gcaatccgct gggctgcgcc gtagccctgg ccaacctcga catctttcgt
     1201 cgcgagaaga cgctcctgca cgttcgaaag cagaccaaat tgctcacgcg tctgctgcgt
     1261 ccgatgacct tgctgagtca tgtgggcgag atacggcaat gtggtttgat ggtcggaatc
     1321 gaattggtgc tggatcgcac gacgcgcact ccctatccgc ttgaaatgcg tatcggccat
     1381 cgcgtggccc aggcctgccg gaaaggcggg cttctgatga gacccctcgg caacgtcatc
     1441 gcactggtcc ctcccctctc cacgagccct caggaattga ctaagatggt ggcaattttg
     1501 cacagcgcca tcgttgatgt gaccgaaggc agtgcatctc cggcgtagaa cgtcccgtca
     1561 ttgcggctga ttcccgcctg ctggaacgcg gcgtcacgcg ttcgccgatg aacatccctc
     1621 gacggttttt gtgcaccccc gccggcgccc gggcggctga gaatcgagtg ctagacttga
     1681 tttcatgctg caccacgaca tcctctcgac ggatttttca gcatgacacg ccgtctgatg
     1741 tcgtggggct ggctcctgct tgtggccctc cccttgacgg ctcaggcgga ggaggcccag
     1801 caatcggatc tcctcctgca acggattcag ttcacggata ccgcccgcga gtccggttca
     1861 tcggcgattc cattttcgat cgcgtctccg gtgctgcacg cctcttttca aaccacctcc
     1921 tttgcgccgc cgaccgcgtc gacgcgtttg aaagacctgg ttcccgattc agaacggccg
     1981 cagacgaaag gcgtggtggc cgcgtcgaca ttcttcaagg ggcagctcac gacggaaggc
     2041 gaggtggcca acaatgcgac gaacgatgcg ggtgttccct ctcgcatcga tcagcgggat
     2101 gacgggacca aacggatggt gcgcatggcg ctaacgggca cgaacggtca gtttcgctac
     2161 ggcatcaact atcggtcggc cggcaaggcg ttcttcaatt cacccgatca gacggtgcgt
     2221 gaaatgtggg gcgaatgggg gatggggatt gccaagcttc gaagttcctc cggtcagacc
     2281 tggaataatg tcgacctgga tcctacccgg gcacggattc aacacacctt caatcgaatc
     2341 ggtctcgcgg tggccaagac ggcttggccc gaactcagtc tgacctatac acgaagcgcc
     2401 ctcgcgagca gtttcgaccc cagtgggtct atcccgcaac ggagtcagag caacagtatg
     2461 gaagccgcgc tctcctatac agggctcacg tggaacgcgc ggctctcatc gagctatatc
     2521 ctgacgaacg acgagacgcg agggggctcc caatcgaccg cctttgccca gaccctggtg
     2581 gcgacctatc ggcccatgaa caccctgacc cttgctccca cgctgagcta ccggacagag
     2641 acccagagct ggtcaggcgc gacgattcag acgcccatgg cgtccctgtc gttgctgtac
     2701 aaacagagtc agcggctatt ggtcagtgcg atgggagggt atacgagcac cagttcgagt
     2761 gatggactga tcgatacgaa cagcatcgtg ggcaagggga tgtttgcgtt gcatctagcc
     2821 ccgatttacg gccatcccac gacgatttct ctggaagccg cctataccaa cacgaccaat
     2881 cggactgtgg cagggctcga taccgaggat ctctccgggc tcgtgcgtat tctggtggcc
     2941 tctctgtagc cccctccccc aagtcctgct tcgctacgct ccaccggctg aggtgtcgcc
     3001 agccgcgagg tgattgatgg tcgacggcgt gagcaagatt cgttcgccgg tcgtcagccc
     3061 ctctcgaatc gtcacctgat taccgatcac gctgtcgata ttgatggcgc gttcctcaag
     3121 ccggccggcg tcatgctgca ccaccaccca ttgtttgccg gagcgttcaa ggatcgcctg
     3181 tttcgggatg acaatcttcg ggcgcatggt gtggcctgcg atggtcactc gtgcaaacat
     3241 ttccggtttc aggcgacgat ccacgttcga cacggacgcg cgaattttaa tggtgcgcgt
     3301 ggccggatcg accacatcgc cgatcacgct gatcgtggcg ggaaactcga tgccagggta
     3361 ggcctcgacg gtcatgggcg cgaccgctcc gacacgaatc cccgagaggt catgttcata
     3421 cacatccgcg acgacctgca aacggtcgag attggcgacg gtaaacaacg gcgtgtccga
     3481 agcagggcct acaatttgtc ccggtgtcac attgcgttcg actacggttc ccgtcaacgg
     3541 gctgcgcaat tcgaaccgtg acgtgatctg ttgttgggca agcggtttgc tgagttcggc
     3601 cgccggcacg cgcagagaca ggagccggcc tttcgcctgt ttgaattccg cccgctcccg
     3661 gttcagatcg ttttccgcat gttccagatc cttcagggac atggcttggg cgtcgtagag
     3721 gtccttcgtc aattcgtagt tgcgttcggc cagtcccagc tcggaaatct cctcgacata
     3781 ggcggcgtag gcggtggcga tgtcggccgc atcgatgacc atcaagatgt cgccggcttt
     3841 gacggcttgg ccgagtttta cccgcacatc cagcaccggt ccctggagag gggatgagat
     3901 tctggaaaac ccatcctcgg cataggtcac tctggccgac agggtcaagg ccggaagcga
     3961 gggcggagtt tcgatcagca tcgattgaat atcaggttgg cggctggcga cgaccggcgg
     4021 ttgaacggtt ttcgacgacg gggcgggtgg atcaggccgc gtacagccag tcaacgtcag
     4081 gccgacaaga atgagcgaca gttggcagag cagttgtttc atggcattcc gttttcgctg
     4141 gtcccgaatc ggtcgtcttg tcgcagaagc caagcgcatg acagcggtag acatcggctg
     4201 gtcggcttga gggggcctgc tccgtacaga ttcactcatg attcctcgat gcatgttgct
     4261 gaaagggcag gagcagccgg ctaggacgaa ggggaacgtt gtgatccgaa cggtctgtgg
     4321 gcagatacgc aaggagatca gcatgttccg ggaagaccaa ctcattcgtc ccggcctact
     4381 cgccaactgt gtcattaaaa catcgacgtc ttttgcctgt caatcgaggc gaccgcatgt
     4441 ctggcgggat gtgctgttcc ggtgactggc gagtgacgtg ctcagcagag ttcgatccgc
     4501 tggccgcgtt cgaccttcaa gcggtcggcc gcacggcctt ctttgaggaa cacttccacg
     4561 tacccgttac tgttgatcag ggcctggggg gtatcgggcg aaccctccgc gtaacttcgc
     4621 accatcccgt cgattgtgat cccgccgatg cggatatagg gctcggggcg tttggtcaga
     4681 ccacgcactt ccttgaggtg gtaggccgtg agattggaga tcagattgcc gaaccgatcg
     4741 atgtagagaa tgtgtcccgc catcacatgt ttgtcccatg ccggttccgc aatcgggagg
     4801 cgttcgtagt tcggcacgag tcgcccgaac gagccgatcg gttggccttt ggtcaaccag
     4861 gcggctgccg gcgcgaagag atcgcgccca tcgaacgtcg ccccgtcgga gtccaatcga
     4921 tactgacggt tctcgatctg tcgcacttcg acgccctgtt cttcctcata gatatgggtg
     4981 aacaggccgt tgtccggccc gatgaagaca tagcgcgacg acgacacgag cagcggccgc
     5041 ctggtgccgc cgacgccggg atccacgacg gccacatgga tcgttccgtc cgggaaatag
     5101 cgataacagg acttgagaag gtacgcggca tccgccacat cgtgcggcgt
//
LOCUS       DAOQRM010000170         5115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_16991_length_5115_cov_17.382411, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000170 DAOQRM010000000
VERSION     DAOQRM010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16991_length_5115_cov_17.382411"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKK81_11355"
     CDS             complement(<1..443)
                     /locus_tag="PKK81_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNV33525.1"
                     /translation="MRINKFFTEQGLCSRREADRLIAEGRVTINGQVAKLGDQVSPQD
                     LVARDGVVLQRGNRLVYLKYHKPVGVTTTTEPHVRRNIIAEIGHTARIFPIGRLDKDS
                     SGLILLTNDGDIVNEILRVEHGHEREYRVEVDREFDETFLVRMAQG"
     gene            complement(620..1552)
                     /gene="speB"
                     /locus_tag="PKK81_11360"
     CDS             complement(620..1552)
                     /gene="speB"
                     /locus_tag="PKK81_11360"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNV33526.1"
                     /translation="MALPSGWLGQADNFLGIDEPWCHPDQAGVYVLPAPYEHTSSYIL
                     GSDRGPSAIIEASQQVELYDETLRYEPYREWGGVATAATLNLDGRVDGPAVQAIQDFV
                     QPHVGRGKFLVTLTGEHTGALGAIRAHARNYPGMCVVQIDAHGDLRQAYQDNPYSHAS
                     VMARVVEDGLPLVQIGVRSISPEEIDLIQKTPRIHTFFAASILDPSGPYEGRAARWIP
                     EVLAACTGPVYLTFDCDGLDASLVPALGTPEPGGLGWYDTLALVNALANGPGIVGMDI
                     SEIAPIEGFVAPQFSIARLIYRMLGRIRAGRRVH"
     gene            complement(1564..2139)
                     /locus_tag="PKK81_11365"
     CDS             complement(1564..2139)
                     /locus_tag="PKK81_11365"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNV33527.1"
                     /translation="MVPTHMFLTRGVGVHKEKLAAFEQALRSAGVAYCNLVSVSSILP
                     PNCKILPRKRGEKLLNPGEITFCVMARSETNERNRLISASIGLAIPTDRDTYGYLSEH
                     HAYGETDEESGEYTEDLAAQMLATTQGIEFDPDVAWKEREQVFKMGGKIVRTLNITQS
                     AVGRPNRWTCVIALAVFIPTENIPKSLRDKA"
     gene            complement(2314..3744)
                     /locus_tag="PKK81_11370"
     CDS             complement(2314..3744)
                     /locus_tag="PKK81_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNV33528.1"
                     /translation="MEDGRPFLIGGRWSHTATTAPVRNPYTGETIAHVCQAGPAEAEA
                     AVQSSVEGAVAMRRLSGYARSTLLQNAAHALQSRQEEFARTMMAEAGKPLTDARREVG
                     RAIQTFSIAGEEAKRIGGEVVPLDWSPGMESYWGVTRRFPIGPILGITPFNFPLNLVV
                     HKVAPALAAGNSILIKPAPQTPLTSLLLAELLLEAGVPPGGLNVLPCDNRVAEQLVVD
                     PRFKLLSFTGSAPVGWMLKAKCGKKKVVLELGGNAAVIVEPDADLDYVAQRCVTGGFS
                     YAGQTCISVQRIFVHESIVAAFTENLVARVERLETGDPGNERTVVGPLIDPGAAQRIE
                     GWIGDAVAQGARVLSGGTRVGSVVRPTVLSQVTPAMKVSCQEVFGPLVTITPYREFDD
                     VLKAVNESEYGLQAGVFTSNIGRIFQAFEQLEVGAVLANEIPTFRADHMPYGGVKDSG
                     IGREGLRYAIEDMTEPKLLVMNLRRP"
     gene            complement(3773..4051)
                     /locus_tag="PKK81_11375"
     CDS             complement(3773..4051)
                     /locus_tag="PKK81_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33529.1"
                     /translation="MASKQAFWSVPAKDGEPDLWVCMSCLSEAFCRKVPMPACPTCHG
                     VSTYEAFTLAAVQDWGTEELIAKAVAAQRDAEAISAAAPATPPLESVQ"
     gene            complement(4124..4777)
                     /locus_tag="PKK81_11380"
     CDS             complement(4124..4777)
                     /locus_tag="PKK81_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNV33530.1"
                     /translation="MTPVTAWLLRVGASCFLFLLVAGAALAASSPAIDHRMRGKADAP
                     VTLIEYSDFTCGYCLKFFKETWPKIQARYVDTGKVRFLYKDYPRADQGPGVTAALAAR
                     CAGDQGTYWPMHDRLFAADGRLDVDSYSQHAKAIGLDQSQFRQCLRDAPHMQAIFHDR
                     DEANSWGFHGTPGFVLMRTAQQPTTKNPAIAIPGAFPFEAFEEEIEKLLAPAGAKQK"
     gene            complement(4774..>5115)
                     /locus_tag="PKK81_11385"
     CDS             complement(4774..>5115)
                     /locus_tag="PKK81_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNV33531.1"
                     /translation="EVLGSKRLGIFTIGGGVPRNWAQQVAPYIEISNTRLGLNVQPPR
                     FHYGVRICPEPDYWGGLSGCTYQEGISWGKFVPPAEGGRFAEVLSDATVVWPLLMVGL
                     LERIRAKSVTA"
BASE COUNT         1057 a         1645 c         1432 g          981 t
ORIGIN      
        1 ccctgggcca tccgcacgag aaacgtttcg tcgaattccc gatccacttc cacgcgatat
       61 tcccgctcat gtccgtgctc gacccgcaag atctcattga cgatgtcgcc gtcgttcgtg
      121 agcagaatga gtccggaaga atctttgtcg agccgtccga tcgggaaaat tctggccgta
      181 tggccgattt cggcgatgat gttccgccgc acatgcggtt cggtggtcgt cgtcactccg
      241 accggtttgt gatacttgag atacaccaag cgattgccgc gctgcagcac aacgccgtcg
      301 cgcgcgacca aatcctgtgg cgagacctgg tcgccgagct tggccacctg gccattgatg
      361 gtcacccgcc cttcggcaat gaggcgatcg gcctcccgcc gtgaacacag gccctgctcg
      421 gtgaaaaatt tattgatgcg catggcgggg aatgaactcg cgtgagccgt ccctcatgaa
      481 gcgtctgtcg tatctcgcaa agagcgtatg gacaaaaacc tcgaccgaaa aacacaggca
      541 gagctctctc attgcctgtg ttctaagtca tgagcgatcc attccgctct ccaacgagag
      601 acgcttcaca atgtgagagt caatgcacgc ggcggccggc acgaatccga cccagcatcc
      661 gatagatcaa gcgggcgatg ctgaactggg gcgcgacaaa cccttcgatg ggcgcgatct
      721 cgctgatgtc catgccgacg atgccgggac cgtttgccaa cgcattcacc agcgcgaggg
      781 tgtcgtacca acccaacccg cccggctcgg gtgttcccaa tgccggcacg agcgacgcat
      841 cgagcccgtc gcaatcgaag gtgaggtaca ccggcccggt gcaggcggcc agaacctcgg
      901 gaatccaacg agcggcccgc ccctcatagg gaccggatgg atcgagaatc gaggcggcaa
      961 aaaatgtatg gatccgtggg gtcttctgaa tgagatcgat ttcctccgga ctgatcgatc
     1021 ggacaccgat ttgtaccagg ggcagcccat cctccaccac acgggccatc acactcgcat
     1081 ggctgtatgg attgtcctgg taggcctggc ggagatcgcc atgggcgtcg atctgcacca
     1141 cacacatacc cggataattc cgggcatgcg cccgaatcgc ccctaaggcg ccggtatgtt
     1201 caccggtcag ggtgaccaaa aatttccccc gaccgacatg cggctgcaca aaatcctgaa
     1261 tagcctggac ggccggacca tcgacccgcc catcgaggtt caatgtggct gccgtcgcca
     1321 cgccgcccca ttcgcggtag ggctcgtagc gtagcgtctc atcgtacaac tcgacctgtt
     1381 gggaggcttc gatgatcgcg gaggggcccc gatcggaccc gaggatataa ctggaggtat
     1441 gttcgtaggg agccgggagt acgtacactc cggcctgatc gggatgacac cagggttcgt
     1501 cgatccccag aaagttgtcg gcttgcccaa gccatccgga cgggagcgcc atgccgcccg
     1561 ccgctaggcc ttgtctcgaa gactcttggg gatgttttcc gtggggataa acaccgccaa
     1621 ggcgatgaca cacgtccatc gattcggacg accgaccgcc gattgcgtga tattcagggt
     1681 ccggacgatt tttccaccca tcttgaacac ctgctcacgc tctttccagg ccacatcggg
     1741 atcgaactcg atgccctgcg tggtcgcaag catctgtgcc gccaagtctt ccgtatattc
     1801 tccggactct tcgtccgttt caccgtacgc atggtgctca gagagatagc cgtacgtatc
     1861 ccgatccgtc ggaatcgcca aaccgatcga cgcggaaatc agccggttgc gctcgttggt
     1921 ctccgatctg gccatgacgc aaaacgtgat ttcaccggga ttcaacaatt tctcgccacg
     1981 cttgcgaggg agaattttgc aattgggcgg gagaatcgac gagacactga cgaggttgca
     2041 ataggccacg ccggcgctac gcaaggcctg ctcgaacgca gccagctttt ccttgtgcac
     2101 cccgacgccc ctcgtcaaaa acatgtgtgt tggtaccatg taggtctccc tcttcatccc
     2161 tcgtcgaaat cactccaccc tgagtgttcg aagaattgtc acaagccttg tgccacgcaa
     2221 ttgatcatgc ggcgacacgc gcgaaagttg tatcaagttt gtggggcatc cgcaatacgg
     2281 cggcagggtt tttttgcctc gccccagggc ctttcagggt ctgcggaggt tcatgaccag
     2341 caacttgggc tcggtcatgt cttcgatcgc ataccgaagc ccttcacgtc cgatcccgga
     2401 atctttcacc ccgccgtagg gcatatggtc cgcgcggaac gtcggaattt cgttcgccag
     2461 gacggcgccg acctccagct gctcaaatgc ttgaaaaatt cgcccgatgt tgctggtgaa
     2521 cacgcccgcc tgcaagccgt actccgactc attgacggcc ttcaatacgt catcgaactc
     2581 gcgatagggc gtaatcgtca ccaacggacc gaacacttcc tgacaggaca ccttcatggc
     2641 gggcgtcacc tgggacaaga ccgtcggccg cacgaccgac cccacccgcg ttccgcccga
     2701 caacacccgg gcaccttggg ccacggcgtc cccgatccag ccttcgatgc gctgcgcggc
     2761 ccccggatca atgagcgggc cgacgaccgt gcgttcattg ccgggatctc cggtttccaa
     2821 ccgctccaca cgggccacga ggttctcggt aaaggctgca acgatcgact cgtgcacaaa
     2881 aatccgttgg accgaaatgc acgtctgccc cgcatagctg aatcccccgg tcacacaacg
     2941 ttgcgccaca taatccagat ccgcgtccgg ctcgacgatc acggcagcat tcccgcccaa
     3001 ttcgagcacc acctttttct tgccgcactt ggccttcaac atccagccca ccggcgcgct
     3061 tccggtaaag ctcagcaact tgaaccgggg atcgacgact aattgctccg ccacccggtt
     3121 atcgcagggc aacacgttga gaccacccgg tggaacgccc gcttcgagaa gcagctccgc
     3181 caggaggagc gatgtcaggg gggtctgcgg agccggtttg atgagaatcg agttaccagc
     3241 cgccaaggcc ggagcgacct tatggacgac aagattcagc gggaaattga acggggtaat
     3301 cccgagaatg ggtccgatgg gaaatcgccg cgtcaccccc cagtacgact ccatcccggg
     3361 agaccaatcg agcgggacca cttcaccccc gatgcgcttg gcttcttccc cagcgatcga
     3421 gaacgtctga atggcacggc cgacctcgcg acgcgcatcg gtcagaggct tgccggcctc
     3481 ggccatcatg gtgcgcgcaa actcttcctg cctggactga agggcgtgag cggcgttctg
     3541 aagcagcgtc gagcgcgcat agccggacaa ccggcgcatc gcgaccgcgc cctcgaccga
     3601 cgactgcaca gccgcttccg cttcggccgg gccggcctgg cagacatgag caatcgtctc
     3661 gcctgtgtag ggattgcgta cgggagcagt cgtagcggtg tgagaccacc gaccaccgat
     3721 cagaaatgga cgtccatctt ccaacggact tactccagga gagggagaat cgctactgca
     3781 cggattccag tggtggtgtt gcgggggctg cggcgctgat cgcctccgca tcgcgctgag
     3841 ccgccaccgc cttggcgatt aattcctcgg ttccccaatc ctgaaccgcc gccaaggtaa
     3901 aggcttcata ggtcgacacg ccgtggcacg tcggacaggc cggcatcggc accttccggc
     3961 aaaacgcctc gctcaaacag gacatgcaca cccataaatc gggctctcca tcctttgcag
     4021 gcacagacca aaaggcttgt ttcgacgcca tagtgcagtt tcctctttcc tggctaaacg
     4081 atgagtctcg tcacagtcct ggctttcttc cgcatgagaa acattatttc tgtttcgccc
     4141 ccgccggcgc gagcagcttc tcaatttctt cctcgaaggc ttcaaagggg aacgcgccgg
     4201 gaatcgcaat agccggattc ttggtcgtcg gctgctgggc cgtgcgcatc aggacgaagc
     4261 cgggtgtgcc gtgaaatccc caggaattcg cttcatcccg gtcgtgaaag atcgcctgca
     4321 tatgcggagc atcgcgcaaa cattgccgga attgcgactg gtcgagtccg atcgccttgg
     4381 catgttggga gtaactgtcc acatccaacc gcccgtctgc cgcgaacaat cggtcatgca
     4441 tcggccaata ggttccctga tcgccggcgc aacgagcggc gagcgcagcg gtgacccctg
     4501 gcccctgatc tgcgcgggga taatccttgt agagaaaccg caccttgccg gtgtcgacat
     4561 accgcgcctg aatcttgggc caggtttctt tgaaaaactt gaggcagtac ccgcacgtga
     4621 agtctgaata ctcgatcagc gtcaccggcg catccgcctt gccccgcata cgatgatcga
     4681 tcgccggcga actcgcagcc agagccgctc ccgcgacaag caagaacagg aaacacgacg
     4741 ccccgacccg aagaagccac gccgtcacgg gggtcatgcc gtgacactct tggcgcggat
     4801 tcgctccagt agcccgacca tgagcagcgg ccagaccacg gtggcatcgc tgagcacttc
     4861 ggcgaaacgt cccccttcag ccggcggcac gaacttgccc caggagatgc cttcttgata
     4921 ggtgcacccg ctcaagccac cccaataatc cggctcggga cagattctca cgccataatg
     4981 aaagcgtggc ggttgcacgt tgagtcccaa acgagtgttg ctgatctcga tatacggcgc
     5041 cacctgttgc gcccaattgc gcggcacccc gccgccgatg gtgaaaatgc cgaggcgctt
     5101 cgaccccagg acttc
//
LOCUS       DAOQRM010000171         5084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_17159_length_5084_cov_15.026248, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000171 DAOQRM010000000
VERSION     DAOQRM010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17159_length_5084_cov_15.026248"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            561..878
                     /locus_tag="PKK81_11390"
     CDS             561..878
                     /locus_tag="PKK81_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33532.1"
                     /translation="MMKRSVEMLLVAALVTGGGGLAFAADPMKEKEATPSIGERITKD
                     AVKGTLMKMDGEFYQIRNTDGELVKVHVDKSTKLDKVVEGDMVKAYVTDKGHVTTLQR
                     VEP"
     gene            1036..1662
                     /locus_tag="PKK81_11395"
     CDS             1036..1662
                     /locus_tag="PKK81_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNV33533.1"
                     /translation="MAAAAPKHFSMLREFHLADVLTLGNAACGLAAVFFAMHYMGSGS
                     PAHFFAATTLAPAALLFDWLDGRVARWRHQQSVLGRELDSLADIISFGVAPAALGFAA
                     GLRGGWDWIALTYFVCCGVSRLARYNVTAESLSAGREKVAYFEGTPIPTTALLTGVLA
                     LAAWQERLGEHLYWGVWTVGPGDLHPLSLMFVLSGTLMISKTLHIPKL"
     gene            1795..3138
                     /locus_tag="PKK81_11400"
     CDS             1795..3138
                     /locus_tag="PKK81_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNV33534.1"
                     /translation="MDTALQYDRLQFAVTATFHYLFPQLTMGLALLLVYLRTRDLLSE
                     GDHYHQVARFWTGIFALSFAFGVVTGIPLEFQFGTNWAKFSEYAGGIVGQTLAMEGVF
                     AFFLESSFLGLLLFGESRVSRRTLWLATVLLWLGSWLSGYFILATNAWMQHPVAYHVA
                     ADGRLTVESLVGLLTNPWLIWQFAHNMTAAVVTGAFVVAAVGALYQLAGRHPALARTC
                     LRTGVVAGAFASVLLITPTGHESARQVFEFQPVKGAAFEGLFRTERGADLVLIGQPNM
                     ETMTIDNPLVVPSALSLLAYDDLYAKVKGLDAFPPREWPDHIALLYYAYHIMVGLGTL
                     LLAVMGLALAWLWRGRLFTARWLLWLLLLSAPFPYVATTAGWMTAELGRQPWLVYGLF
                     RTEEGISPLVHSGNALFTLLGFLGIYLGLGLLFVLLFVETLRHGPTEPVVGQVGS"
     gene            3140..4189
                     /gene="cydB"
                     /locus_tag="PKK81_11405"
     CDS             3140..4189
                     /gene="cydB"
                     /locus_tag="PKK81_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNV33535.1"
                     /translation="METIWYALVLFVLTTYVVLDGYDFGVGILSPFVARQESERRLVR
                     ETIGPVWTGNEVWVIAGSALLFLAFPKAFASGFSGFYLALMIMLWLLMGRGLAFELRE
                     HVNHALWRTFWDTIFFLANLLLAFVVGLVVGNLLRGVPLNASGYFFLPLWTDFSPGSM
                     PGILDWFTLLTGLTLVALLTLQGASFLVMKTRGELQARAEALAKSATVLLVLLLVTVV
                     VALPSVQPAFGAHYVAAPIGFVWPLTAVVALLVRHVSAVRQRPFVGFVASSVSVASLL
                     VAAAWGIFPNLLIATTGQANSLTSLNAATDRTGLEAGLWWVPPGLALVILYQVQIHRL
                     FGRPAKPESPAMARH"
     gene            4256..>5084
                     /locus_tag="PKK81_11410"
     CDS             4256..>5084
                     /locus_tag="PKK81_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV33536.1"
                     /translation="MARPLRLSFPGAVYHVTSRGNARQDIVADDRDRSQWLRLLAHVV
                     DRYGWMCHAYCLMDNHYHLLIETPKPNLSLGMRQLNGRYTQAYNLRHEQVGHVFQGRF
                     TAILVEKDAHLLELCRYVVLNPVRAKIVSHPRLWAWSSYRATVGETKAPGWLTTDWIL
                     SQFGQREGPAQERYRTFVAEGRGGPVPWEQLTGQIYLGSEKFVAQHQPNRVIRDIPRR
                     QTQAQRPSLETLFQRKGDLEKTIHQAYRQYGYRLAEIADHLGVHAATVSRRLKRAEQE
                     "
BASE COUNT          840 a         1498 c         1625 g         1121 t
ORIGIN      
        1 tttaccgctc aaggggtcgt tgcggagatc cgcgccaaga tgcgtatcct gtccgaggaa
       61 tatgatgccg aaggactcac gatacgagtg cgatcgaccc ctgagaacct gactgccatc
      121 aaaaagaagc tcgcgcgatg aggcgcggat tccaccccat ttagggtcag gtcttgcaat
      181 caaacaattg atcgagggag gtcagcatga aatcttttcc ggggggcatc ttaggcggcg
      241 atgtcaggcc ccgggcggtt tgagtggaag tacggcccgg agaagatggt tcagcgggct
      301 gatctgtcag tacgggtatc agtcggctgt gattgcggaa cacttgggga acaatcgccg
      361 cccaccgaag ctttcatcag ccacctcgtt tcctctcaac atggaatccc tggttcgaat
      421 ttagtcatcc gcctatccgg cctgcgttcc ccacatgact cacagaggcc cggtggcagg
      481 ggattttccc agtatgtcct ccttctcatg agggttacgg taagagttga caccgactca
      541 gttacaacat gggagggtat atgatgaaac gaagtgttga aatgctgctc gtcgcagccc
      601 tggttaccgg agggggagga ctcgcgtttg cggccgatcc catgaaagag aaggaagcca
      661 cgccgagcat cggtgagcgg atcaccaagg atgccgtcaa gggcaccctc atgaagatgg
      721 acggcgagtt ttatcagatc agaaatacag acggggaact cgtcaaagtg catgtggaca
      781 agagcacgaa gctcgacaag gtggtcgaag gcgatatggt caaggcctat gtcaccgata
      841 aaggccacgt gaccacgttg caacgggttg agccctaatc ccatttcgaa gtagtccggg
      901 gcggtaggct tgcgggcctg ccgcccggct tggtcgggca atgaagacga caagaagttc
      961 tgcttgactg caccccactc gcttcagagt tcagattctg gacattcggc gcaatcccgc
     1021 gtagattttc ctgccgtggc cgctgcagcc ccgaaacatt tttcgatgct ccgcgagttt
     1081 catctggccg acgtgctcac gctgggcaac gccgcctgcg ggctcgcggc cgtgttcttc
     1141 gccatgcact acatgggcag cgggtctccg gcccatttct tcgctgccac gaccctggcg
     1201 ccggcggccc tgctcttcga ttggttggac ggccgggttg ctcgatggcg gcatcagcag
     1261 tcagtgctcg ggcgggagtt ggattcgttg gccgacatca tctccttcgg cgtcgcaccg
     1321 gcggcactcg gattcgccgc cggtctaaga ggagggtggg actggatcgc gctgacgtac
     1381 ttcgtctgct gcggggtgag tcgcctggcc cggtacaacg tgactgccga gagcctctct
     1441 gcgggtaggg agaaggtggc ctatttcgaa ggcaccccca ttcccaccac cgcgctgttg
     1501 accggcgtcc tcgccctggc cgcctggcag gagcgactcg gcgaacatct ctattggggt
     1561 gtctggaccg tcgggcccgg cgacctgcat cccctgtccc tgatgttcgt cctctccggc
     1621 accctgatga tcagcaaaac cctccacatc cccaaactgt agacgtcgca ctatttctat
     1681 ttagggtcag gtcttgcaat caaacacttc atcggccctg gggaagtcac tagcttcccc
     1741 atcaggtctg atcagtcaca atgtcgcggg ccagttcaag ggggtggtgt agtgatggat
     1801 accgcgctgc aatatgaccg gctgcaattc gccgtcacgg ccacgtttca ctacctcttt
     1861 cctcagctca cgatgggact cgccctgctg ctggtgtatc tgcgcacgcg tgacctgctc
     1921 agtgaggggg accattatca ccaggtcgcg cgattctgga ccggcatctt tgcgctgagc
     1981 ttcgcgttcg gtgtcgtcac gggcattccg ctcgaatttc agtttggcac caactgggcc
     2041 aagttctccg aatacgcagg cggcatcgtc gggcagacgc tggcgatgga aggggtgttc
     2101 gccttttttc tggaatcgtc gttcctcggc ctgctcctgt tcggcgaatc gcgcgtcagc
     2161 cgtcgcacgt tgtggttggc gacggtactg ttgtggctcg gctcctggtt gtccggctat
     2221 tttattctgg cgacgaacgc ctggatgcaa catcctgtgg cctatcacgt ggcggccgac
     2281 ggccgtctga cggtcgagag tctggtcggg ctgctcacga atccctggct gatctggcaa
     2341 ttcgcccata acatgacggc ggcggtcgtc accggcgcgt tcgtggtggc tgcggtgggt
     2401 gccctctacc agctggcggg gaggcatccc gcgctggcca ggacctgtct gcgcacgggc
     2461 gttgtggcgg gcgcgttcgc ctcggtgctg ctcatcacac cgacgggaca tgagagcgcg
     2521 cggcaggtgt tcgagttcca gccggtcaag ggcgctgcct tcgagggcct ctttcgcacc
     2581 gagcgcggcg ccgacctcgt gctgatcggc cagcccaaca tggaaaccat gacgatcgat
     2641 aatccgctcg tcgtgccgtc ggcgctgagc ctgctcgcct atgacgatct ttacgcgaag
     2701 gtgaaggggc tggatgcctt tcccccgcgc gagtggccgg accacattgc gctgctgtat
     2761 tacgcgtacc acatcatggt cgggctcggc acgctcctgc tcgcggtgat gggattggcc
     2821 ctggcgtggc tgtggcgtgg ccggctgttc accgcccgct ggttgctctg gctcctgctg
     2881 ttgagcgctc cttttcccta tgtcgcgacg acggccggct ggatgacggc ggagctcggc
     2941 cgccaaccct ggctggtgta cgggctcttt cgcacggagg agggcatctc gccgctggtc
     3001 cattccggca atgcgctctt caccttgctc ggcttcctgg ggatctatct cgggttgggg
     3061 ctgctgttcg tcctcttgtt cgtggaaact ctgcgtcacg ggccgacgga gccggttgtg
     3121 gggcaggtgg ggagctaacg tggaaacgat ctggtatgcc ctggtgctgt tcgtgttgac
     3181 cacctatgtg gtgctcgacg gctatgactt cggcgtcggg atcctgtcgc cattcgtggc
     3241 ccggcaggag agcgaacgcc gcctggtgcg ggagacgatc ggaccagtgt ggaccggcaa
     3301 cgaagtgtgg gtgattgccg gcagcgctct cttgttcctg gcctttccga aggcctttgc
     3361 gtcggggttc agcgggttct acttggcctt gatgatcatg ctgtggctcc tgatgggcag
     3421 ggggttggct ttcgagctgc gggagcatgt caatcacgcg ttgtggcgca cgttctggga
     3481 taccatcttt ttcctggcca atctgctgct ggccttcgtg gtggggctgg tggtggggaa
     3541 tctgctgcgg ggcgttcctt tgaatgcgag cggctatttc ttccttccgc tctggaccga
     3601 cttcagcccc ggttcgatgc ccggcatttt ggattggttc actctgctga cggggctgac
     3661 cctggtcgcc ctgttgacgt tgcagggcgc gagtttcctg gtcatgaaga cgcgcgggga
     3721 gttgcaggca agggcggagg cgctggcgaa gtccgccacc gtcttgctgg tgctgttgct
     3781 cgtgaccgtg gtcgtggcgc tgccctccgt gcagccagcg ttcggcgcgc attatgtggc
     3841 cgctcccatc ggctttgtct ggcccctgac ggcggtggtc gcgctgctgg tccggcacgt
     3901 gagcgctgtc cgtcaacggc cgttcgttgg cttcgtggcg tcgagtgtgt cggtcgcgag
     3961 cctattggtc gcggcggctt gggggatttt ccccaatctg ttgattgcga cgaccggaca
     4021 ggccaacagt ctcacgagcc tcaacgccgc aaccgaccgg accggtctcg aagccggtct
     4081 ctggtgggtg cccccgggcc tcgccctggt catactgtat caagtgcaga ttcatcgcct
     4141 cttcggccgc ccggccaagc ctgaatctcc tgcgatggcg cgccactagt ctagccttcc
     4201 cttttttggg gtcaggtctt gcaattaaac aatctcacgc cgtacactcc cgctcatggc
     4261 acgtccccta cgtctctcat tccccggcgc cgtctaccat gtcacgagtc ggggcaacgc
     4321 ccggcaagac atcgtggctg acgaccgtga ccgctcccaa tggctgcgcc tgctggccca
     4381 tgtcgtcgat cgctatgggt ggatgtgcca tgcctactgt ctgatggaca atcactatca
     4441 tctgttgatc gagacgccga agccgaatct ctcgctcggc atgcggcagt tgaatggccg
     4501 ctacacgcag gcctacaacc tccggcacga gcaggtcggg catgtgtttc aagggcgctt
     4561 cacggcgatt ctggtggaaa aagacgcgca tctgcttgaa ttgtgccggt acgtcgtgct
     4621 gaatccggtc cgtgcgaaga tcgtgtctca tccccggtta tgggcctgga gcagttaccg
     4681 ggcgacggtg ggggagacga aggcgcctgg ctggctgacg acggactgga ttctgagcca
     4741 attcggccaa cgcgagggac cggcgcagga gcggtatcgc acctttgtgg cggaggggcg
     4801 gggtgggcca gttccttggg aacagctcac cggccagatc tatctggggt cggaaaaatt
     4861 cgtggcgcag caccaaccga atcgggtgat ccgcgacatt ccgcgccgac agacgcaagc
     4921 ccagcgcccc tcattggaaa cgctgtttca gcggaagggt gacctggaga agaccatcca
     4981 tcaggcctat cggcagtacg ggtatcgatt ggccgaaatt gcggaccatc tgggtgtaca
     5041 tgccgccacg gtgagccgcc gcttgaagcg ggccgagcag gaaa
//
LOCUS       DAOQRM010000172         5071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_17231_length_5071_cov_15.547249, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000172 DAOQRM010000000
VERSION     DAOQRM010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17231_length_5071_cov_15.547249"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PKK81_11415"
     CDS             <1..1213
                     /locus_tag="PKK81_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNV33537.1"
                     /translation="TTSRYQDFVPPHRLLMGPGPSMVHPRVLQALSQPLLGHLDPVFL
                     TLMNDIQALLRATFHTQNEFTIALSGTGSAGMEAVIANLIEPGDRAIVGVNGVFGTRL
                     ATMIERSGGLPIRIEAPWGATIPIEALEQELARSAPVKALVLVHAETSTGALQPLEDV
                     GALCRAHDALLIVDAVTSLGGIPVEVDTWGIDACYSGTQKCLSCPPGLAPLTVSPRGM
                     GAIRQRRAPCHSWYLDLSLIADYWNDHSRAYHHTAPISMLYGLREALRLVHEEGLPAR
                     AARHQVNSDALMAGLQTLGLNPLPPPGHRLPMLNCVTLPDGIDEAMMRTQLLQQYGIE
                     IGGGLGPLRGRVWRIGLMGESCRQAHVLTLLNALEEIFAGQGRLTQPGRAVQAATETY
                     THSLGSARRGA"
     gene            1210..1812
                     /locus_tag="PKK81_11420"
     CDS             1210..1812
                     /locus_tag="PKK81_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV33538.1"
                     /translation="MSRNGLWIVAAGVAGFVAAITYWIVALAVAESHKAEMVPPERVT
                     SFIHAVIDANRANYTQNVVDKLHNQGVVEALEHWKEEKGLPLPAQFLLESGRLVAQKD
                     MKLSFRLASLTPIYVWNGPNSEFERRGLEVVMKAPEKPFTGFYQQAGVRYFQGIYADR
                     AVSESCVSCHNAHVNSPRRDYKLNDIMGGVIVTIPISEAP"
     gene            1809..3017
                     /locus_tag="PKK81_11425"
     CDS             1809..3017
                     /locus_tag="PKK81_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV33539.1"
                     /translation="MTIAIRHIRILDGLGHRIERGTLLIEGTRIQAVGPEQDVRLPKG
                     AQRIDGRGLTVLPGLIDCHVHFCLGAEADVVAAVEGESSSVTLLKAAELARRTLHAGF
                     TTVRDVGFRDHAVFALKQAIESGLTPGPRILAAGLAICMPGGHARFIGREADGVEAVR
                     AAVLDQLNAGAEVIKVIASGGVLTPGTSPDDAQMTVAELSAAVEVAVAHGRHVAAHAH
                     GATGMKNALRAGVHSIEHATLMDDDAAEMMTQRHVYMVPTLSALATTAACPTGCGIPD
                     SARSKARNMVKQHERSFRAALRRGVPIAMGTDAGTPFNYHGDNAQELERMVALGMTPM
                     EAIMTSTSAAARLLGLIQDIGSIAAGKQADLLVVEGNPLRNIGVLLKRERIAGVMQRG
                     RFVAGPLSRA"
     gene            complement(3043..3681)
                     /locus_tag="PKK81_11430"
     CDS             complement(3043..3681)
                     /locus_tag="PKK81_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNV33540.1"
                     /translation="MPRLDYYRVLGVSRDISDDDIKKAYRKLVFEHHPDRNPHNKQAE
                     EKIREINSAYEVLGDPESRRTYDRLHWGEEARAETVDPSLILEEMEKKLFDEGRKEVF
                     AVLMKMVPRIKSELALIRERTVAHQGYDTFKESIVEARGAEVLDEFVTSDMEQRKQRL
                     LEVAVQMMVSQAVVAKGDEGGIRALRGRLEEMFRKGRINGFATALELLYERR"
     gene            3790..5070
                     /locus_tag="PKK81_11435"
     CDS             3790..5070
                     /locus_tag="PKK81_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV33541.1"
                     /translation="MAWAQGVTRYQWLVLFVAWLGWVFDAMDATIYAIVLHPALHELL
                     QAGAASGAVTDEQIGWYGGMVFSIFLIGWAIGGIGFGMAADYFGRTKTLIITILIYAV
                     FTGLAAFAQEWWHLAIYRFLTALGIGGEWAAGAAIVAETWPEDKRARAAGILQSAWAA
                     GFFLAAGFNLLLSDYGWRLLFLVGILPAFVALLVRWWVKEPERWVQAHEQEAQSVRNS
                     FMHLAELFQPALRRNTWVGSTLAFVAVFGLWGATNWAPTLIRAMPDLQGQDAATLTQK
                     VSYAIMALNAGSLFGYLGFGPLAERFGRLPVFGLMCLGSFIMLPATYFVPHSYAEVLL
                     LLPLLGFFNNGIFSGFPIYLPELYPTRLRATGSGFCFNAGRVLASAAPFVTGWLVTLL
                     GSFNNAASTIALIYLLGLAVLPFATETNGKPLPE"
BASE COUNT          942 a         1644 c         1523 g          962 t
ORIGIN      
        1 tacaacctcc cggtatcagg actttgttcc gccgcatcgc ctgctcatgg gcccgggccc
       61 gagcatggtc catccccggg tgttgcaggc cctgtcacag ccgttgctcg gacacctcga
      121 cccggtcttt ctcactctga tgaacgacat tcaagccttg ctccgcgcca ccttccatac
      181 ccaaaacgag tttacgattg ccttgtccgg aaccggatcg gcaggcatgg aagcggtgat
      241 cgccaatctc atcgaacccg gcgatcgtgc gattgtggga gtcaacggcg tgttcggcac
      301 caggctggcg accatgatcg aacgaagcgg cggcctcccg atccgcatcg aagcgccctg
      361 gggagcgacg attcccatcg aggcgctgga acaggaactg gcgcggtctg ctccggtcaa
      421 agccctggtc ctggtccatg cggaaacgtc aaccggcgcc ctccaacccc ttgaggacgt
      481 gggggcgctg tgccgcgcgc acgatgcatt attgatcgtc gacgccgtca cctccctggg
      541 aggcatcccg gtcgaggtcg atacctgggg aatcgacgcc tgctacagcg gcacgcagaa
      601 gtgcttgagc tgtcccccag gcctggcccc gctcaccgtc agcccgcgag gcatgggcgc
      661 catccggcag cgacgtgctc cctgccacag ctggtacctc gatctgtcgc tcatcgccga
      721 ctattggaac gaccacagcc gtgcctatca ccacaccgcc ccgatctcga tgctctacgg
      781 gcttcgggaa gccctgcggc tggtgcacga agagggactc ccggcgcgtg ccgcgcgaca
      841 tcaggtaaac agtgatgcat tgatggccgg gctccaaact ctgggcctga atcccctgcc
      901 cccgccgggc catcgactcc cgatgctcaa ctgtgtcacg ctacccgacg gaatcgacga
      961 agcgatgatg aggacgcaac tgctgcaaca gtacggcatc gaaatcggcg gcggactggg
     1021 accgttacgg gggcgggtct ggcgcatcgg cctcatgggc gaatcgtgcc gacaggcgca
     1081 cgtgctgacg ctgctcaacg ccctggaaga aatcttcgca ggccagggac ggctgacgca
     1141 gccgggccgc gccgtccagg cagcgacaga aacctatacc cattcactag gatcggcgag
     1201 gaggggtgca tgagtcgaaa cgggttatgg atcgtggcgg caggagtggc cggcttcgtg
     1261 gcagccatca cctactggat cgtcgccctc gcggtcgcag aatcacacaa ggcggagatg
     1321 gtcccccccg agcgtgtgac cagcttcatt catgcggtca tcgacgcgaa tcgagccaac
     1381 tacacacaga acgtcgtgga caagctccat aaccagggcg tcgtcgaggc actcgaacat
     1441 tggaaagaag agaagggcct cccgctcccg gcccaattcc tcctcgaatc cgggcgcctg
     1501 gtggcgcaaa aggatatgaa actcagtttc cgtctggcca gcctcacccc gatatatgtg
     1561 tggaacggac cgaacagcga gttcgaacga cgcgggcttg aggtcgtgat gaaggcaccc
     1621 gagaaaccct tcaccggatt ctaccaacag gcgggcgtcc gctacttcca aggcatctac
     1681 gcagaccggg cggtgtccga aagctgtgtg agctgccaca atgcccacgt aaacagcccg
     1741 cgacgggact acaaactcaa cgacatcatg ggcggcgtca tcgtcaccat tccgatcagt
     1801 gaggcgccat gaccatcgcc atccgccaca tccgcattct ggacggcctc ggtcaccgga
     1861 ttgagcgagg taccctcctc atcgagggca cccgcatcca ggccgtcggg ccggaacagg
     1921 acgttcggct ccccaagggc gcgcaacgaa tcgacggccg cggcctgacc gtccttcccg
     1981 gcctgatcga ttgccacgtt cacttttgtt tgggagcgga ggcggatgtc gtggctgcgg
     2041 tggagggaga atcttcgtcc gtcaccctgc tcaaagccgc cgagctggcg cgccgcactc
     2101 tccacgccgg gttcaccact gtccgggatg tgggattcag ggaccatgcg gtgttcgccc
     2161 tgaaacaggc catcgagtcc ggactgacgc cggggccacg tattcttgcc gccggtctcg
     2221 cgatctgcat gcccggcgga catgcgcgct tcatcggccg cgaagccgac ggagtcgagg
     2281 ccgtccgcgc cgccgtcttg gatcaactca atgccggcgc cgaagtcatc aaagtcattg
     2341 cctccggcgg cgtgctcacc cccggcacct ctccggacga cgcgcagatg accgtggcag
     2401 aactgtccgc tgccgtcgaa gtggccgtcg cccacgggcg acatgtggcc gcccacgccc
     2461 acggcgcaac cgggatgaag aacgcgctcc gcgcaggcgt ccactccatc gaacatgcca
     2521 ccctcatgga cgacgacgcg gcagagatga tgacgcaacg ccacgtctac atggtaccta
     2581 cgctgtcggc cctcgccacc acagcggcct gcccgaccgg ctgcggcatt cccgacagcg
     2641 cccgctccaa agcgaggaac atggtgaagc agcacgagag aagtttccgc gccgccctcc
     2701 gccggggtgt tccgatcgcc atgggcacgg atgccggaac gccgttcaac tatcatgggg
     2761 acaatgctca ggaattggaa cggatggtcg cgttgggcat gacgccgatg gaagcgatca
     2821 tgaccagcac ctccgccgcc gcgcggttac tgggcctgat tcaagacatc ggatccatcg
     2881 cggcgggaaa acaggcggac ctgctggtcg tggaaggcaa tcctctcaga aatatcggag
     2941 tactgctgaa acgagagcgg attgcgggag tgatgcaacg cggtcggttc gtggcgggtc
     3001 cgctctcacg agcgtgaaac gtgacacgaa agacgtgggt cgttaccgtc tttcatacag
     3061 taattccaag gccgtcgcga agccgttgat ccggcccttg cggaacattt cctctaaccg
     3121 gccgcgaagc gcacgaatcc cgccctcgtc tcccttcgcg accaccgcct gcgacaccat
     3181 catctgcacc gcgacctcaa gcaagcgctg tttccgttgc tccatatcgg aagtcacgaa
     3241 ctcatccagg acttccgccc cgcgcgcttc cacgatggac tccttgaagg tgtcataccc
     3301 ctgatgcgcc accgtgcgtt cacgaatgag ggccagttcc gatttgatcc gtggcaccat
     3361 cttcatcagg acggcgaaga cttccttccg cccttcgtca aagagctttt tctccatctc
     3421 ctcaaggatc agggacggat ccaccgtctc ggcccgcgcc tcttctcccc aatgcaggcg
     3481 atcataggtt ctccggctct cgggatcgcc caggacctca taggcggagt tgatctcgcg
     3541 gatcttctcc tctgcctgct tgttgtgggg attgcggtcg ggatggtgct cgaaaacgag
     3601 cttccggtag gcttttttga tgtcgtcgtc ggaaatgtcg cgcgacacac cgaggacacg
     3661 ataataatcc aggcgaggca tacagggcga gactatacca tgccgcttcg ccggcttcac
     3721 cttgacagcg tcgggactcc cccgtagcct ggcgcggagg cagcctgttc gcaggggagg
     3781 gagttcggca tggcgtgggc acaaggcgtc acgaggtatc aatggctggt cctgttcgtc
     3841 gcctggctgg gttgggtatt cgacgccatg gacgcaacga tctacgcgat cgtgctgcat
     3901 cctgccttac atgagcttct acaagccggg gccgccagcg gagccgtcac ggacgaacag
     3961 atcgggtggt acggcggcat ggtgttttct attttcctga tcgggtgggc catcggagga
     4021 atcggcttcg ggatggccgc ggactatttc ggccggacca agacgttgat catcacgatt
     4081 ttgatatatg ccgtcttcac cggcctcgcc gcgtttgctc aagaatggtg gcatctggcc
     4141 atctatcgct ttctgaccgc gctcggcatc ggcggcgaat gggccgccgg cgcggccatt
     4201 gtggccgaga cctggcctga agacaaacgt gcccgggcag ccgggattct acagtccgca
     4261 tgggccgccg gatttttcct ggccgccggc ttcaacctgc tcctgagcga ctacggatgg
     4321 cgactcctct tcctcgtggg catcctgccc gcctttgtgg ccttactcgt ccgttggtgg
     4381 gtgaaagaac cggagcgatg ggtgcaggcc cacgaacagg aggcacaatc cgttcgcaac
     4441 agcttcatgc acctcgccga gttgtttcaa ccggcattac gccgcaacac ctgggtcggc
     4501 tcaaccctgg cgttcgtggc ggtcttcggc ctgtggggcg cgaccaattg ggcccccacc
     4561 ttgatccggg ccatgccgga cctccagggg caggatgccg ccacgctgac acagaaggtc
     4621 agttacgcga tcatggcgct caatgcgggc tcgttgttcg ggtatctggg attcgggccg
     4681 ttggccgaac ggttcggccg cctgcccgta ttcggcttga tgtgcctcgg cagcttcatc
     4741 atgctgccgg cgacctattt tgtcccgcac agttatgcag aagtgttgct gttactcccg
     4801 ctgctggggt tcttcaacaa tggcattttc agcggctttc cgatttactt gcctgagctc
     4861 tatccgacac gactccgtgc aaccggctca ggattttgct tcaacgcagg ccgcgtgctc
     4921 gcctcggccg cacccttcgt caccgggtgg ctcgtcaccc tgctgggatc gttcaacaac
     4981 gcagcgagca ccatcgcgct gatctatctg ttgggattgg ccgtactgcc cttcgcgacc
     5041 gagacgaatg gaaagccgtt accggagtga c
//
LOCUS       DAOQRM010000173         5065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_17255_length_5065_cov_14.075050, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000173 DAOQRM010000000
VERSION     DAOQRM010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17255_length_5065_cov_14.075050"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /gene="pilQ"
                     /locus_tag="PKK81_11440"
     CDS             <1..1140
                     /gene="pilQ"
                     /locus_tag="PKK81_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNV33542.1"
                     /translation="VKSKVTMKLANVPWDQALEMLLKMNALGMIRQGNIVWVDTLQNI
                     AKQQDEEARAKDSKAKAEPIVTRVFYIRNLNATEVQTSLRQNLSPRGTMTVSAASNAL
                     IISDTESKLEVLRQLLDGVDLEVPQVQIEARIVQADTTYTRSLGVQWGIQNVNQLGSS
                     SGTSAFKTGTTGAFGAQVSDFLINLPANPGLPSVPGAGFSIGKTDGAMLDVRLSAGEL
                     LGLTKVIAAPKITTLDKRDAKIAQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSR
                     DPKEIGKQILMKVRATRNAVGARSNPAGPSIDRREATTQVLVRDGETMVIGGVFVDTQ
                     SNNVAGIPYLSRIPVLGWLFKNKTENVSKQELLIFLTPTIVRSTT"
     gene            1413..2570
                     /gene="aroB"
                     /locus_tag="PKK81_11445"
     CDS             1413..2570
                     /gene="aroB"
                     /locus_tag="PKK81_11445"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNV33543.1"
                     /translation="MTRSSSDRPSHSIHVELGARSYAIVIRRDLLREIGEELKRLKCA
                     GKVGVVTDRNLAKHYLTQVTRRLKAAGFTVVPVVLPPGERTKTLRSIGTVMDALVNAR
                     FERSSTLLALGGGVIGDITGFAAAIYQRGIPFVQVPTSLVAQVDSSVGGKTGVDHPKG
                     KNLIGAFNQPRAVFIDPAMLATLPPREWVAGLAEVIKYGVIADAAFFEYLEQNMESIQ
                     KLTDDAVAHIVKRSCEIKAQVVSEDEREADRRRILNFGHTIGHALESLGGYRGFIHGE
                     AVAVGMVYEADLARHLGYCGAEVVARLRALVKAAGLPDRLPKVAFNALWGAMQQDKKV
                     SAGTIFCVVPEQIGAVRIVPLEKATTRAWFQDVHTREAAELKRARPVGRAR"
     gene            2578..3837
                     /locus_tag="PKK81_11450"
     CDS             2578..3837
                     /locus_tag="PKK81_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HNV33544.1"
                     /translation="MATKRTLTQVEQALREKTREVDVLHRISESISNTLDLEAVLRHI
                     VDMVVEVTKADACLLYLLSDNRDELILRASKNPHPKLIGRITIGMGEGITGWVARERT
                     RVVIPNSASDDTRFKFFHNLPEDRYQAFVSVPITTKQEVVGVINVQHKRSRRYRPDEL
                     ALLSTIATQVGGAIENARLYDQMTRKALQLDTLSQVSETVSSNRLIEDVLQLIVTMTA
                     QMMGSKICSIILLDQPTGELRIAATQSLSEQYRRKPNVKIGQSISGRAVQDRRPIIVP
                     DVIKEGTYMYPDMAAKEGLCSLLCVPMLVREKAIGVINTYTSKPHTFTAEDVKLMQAI
                     ANQAAISIEHTTLLEKSFEMQEALQVRKLLDRAKGYLMRSKRLSEEDAFKLIQRQSMD
                     LRKSMREIAEAILLAGEIEERADKGRS"
     gene            4180..4524
                     /locus_tag="PKK81_11455"
     CDS             4180..4524
                     /locus_tag="PKK81_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33545.1"
                     /translation="MTSACATGQYTPLVGADRDEVEQLKENVYRVEYRVSSFTSQEQL
                     DAYLRRRCAELTLREGYDFFHLAQRADVLALSRRTSMTVTMYKGKKPVGADDVYDAKE
                     VLAQPDLLSGSQ"
     gene            complement(4541..>5065)
                     /locus_tag="PKK81_11460"
     CDS             complement(4541..>5065)
                     /locus_tag="PKK81_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33546.1"
                     /translation="AQPDQRVRFTMQDDGLTTQGTLYVYKTTLRVNLSHYRSTSGDGQ
                     TKPASLILSFTPPQALVRADAPQPWMTVEPEQPRIAVSLETLSQLPAPSPVAADHNTT
                     AEAAPTQTSPTAEQSRLQQELQSTKDVVVKQAEELQKLKAELESVRRQLAEKDVAASK
                     PKPKAAPRKPTATP"
BASE COUNT         1022 a         1378 c         1607 g         1058 t
ORIGIN      
        1 gtcaaaagca aagtcacgat gaagctggcg aatgtgccgt gggatcaggc cctcgaaatg
       61 ttgctgaaaa tgaatgcgtt ggggatgatt cgccagggca acatcgtgtg ggtggatacc
      121 ctgcagaata ttgccaagca gcaggatgaa gaagcgcggg caaaggactc gaaggcgaag
      181 gcagagccga tcgtcacccg ggtattctat atccggaatc tcaacgccac ggaagtgcaa
      241 acgtcgttac gccagaactt gagtccgcgc gggaccatga cggtcagcgc cgcgagcaat
      301 gccctcatca tcagcgacac ggagtccaag ctggaagtcc tgcggcagtt gctggacggc
      361 gtcgatctcg aagtaccgca agtgcagatt gaggcccgca tcgttcaagc cgacacgacc
      421 tatacccgct cactcggtgt gcagtggggt atccagaacg tcaaccagct gggaagctcc
      481 agcggaacgt ctgccttcaa gaccggaacc accggggcat tcggcgccca agtctcggac
      541 ttcctgatca acttgccggc taaccccgga ttgccgtcgg tgccgggtgc cggattctcg
      601 atcgggaaaa ccgacggggc gatgttggat gtgcggttgt cggccggtga attgctgggc
      661 ttgaccaaag tgattgcggc gccgaagatc acgacgctgg acaagcggga cgccaagatt
      721 gcccagggtg aatcgatccc attccagacc acgtccttgc aggggaccca gacgaccttc
      781 gtggatgcga acctggagtt gaacgtgacg ccgcaaatta cctctcgcga cccgaaagag
      841 atcggcaagc agattctcat gaaggtgcgg gccacccgga acgccgtcgg cgcccggagc
      901 aacccggccg gtccgagcat cgatcgtcgt gaagcgacga cccaggtgct ggtgcgtgac
      961 ggggaaacca tggtcatcgg cggtgtcttc gtcgatactc agtcgaacaa cgtggcagga
     1021 attccctatc tgtcacgtat cccggtcctg ggctggttgt tcaagaacaa gaccgagaac
     1081 gtctccaagc aagagttgct gatcttcctg acgccgacga tcgtgcgctc gacaacttga
     1141 caggtcccag cgcggcaagt taataatcgc ccccattgaa gatggtacgc tatctcaatg
     1201 ggggcgaatc ctgcggaacg tgtcttctcc ccctatctga tagcgctcct tccgggtgtc
     1261 ttcttcctct agatatcgca ggttacgatc cagtctcctt cgcctcatgg cctatcgggg
     1321 tatctggatt gttcgcgtgg ctcgcttccc gggccatgcc gccatcatgg acttgagact
     1381 cagccctgcg tccacacgct gcgtgatacc tgatgacacg ttcttctagt gataggccgt
     1441 cccactccat tcatgtcgaa ctcggtgcgc gaagttacgc catcgtcatt cgccgcgacc
     1501 ttcttcggga gatcggggag gagctgaagc gtctcaaatg cgcgggcaag gtaggggtcg
     1561 tgaccgatcg caatcttgcc aagcactatc tcacgcaggt gactcgcagg ctgaaggccg
     1621 ccgggtttac ggtcgttccc gtcgtgctgc ctcccgggga gcggacaaaa acccttcgct
     1681 ccatcgggac ggtcatggat gccctcgtga atgccagatt tgagcgttcc tcgacgttgc
     1741 tggccttggg tgggggtgtc atcggcgaca tcacgggatt tgccgccgca atctatcagc
     1801 gtggcatccc gttcgtgcaa gtgccgacga gtctggtggc ccaggtcgat tcaagtgtcg
     1861 gcgggaagac gggtgtcgat caccccaagg ggaaaaattt gatcggggcc ttcaaccagc
     1921 cgcgcgcggt gttcatcgat cctgcgatgc tggcgactct gccgccgcgc gagtgggtgg
     1981 ctggtctggc tgaggtcatc aaatatggtg tgattgcgga cgcggccttc tttgagtatc
     2041 tggaacagaa catggaatcg atccagaagt tgaccgatga cgccgttgcg cacatcgtga
     2101 agcggtcctg tgagatcaag gcacaggtgg tgtcggagga tgagcgtgag gccgaccgac
     2161 gccgcatttt gaacttcggc catacgatcg ggcatgcgtt ggaatccctc ggcgggtatc
     2221 ggggcttcat ccatggggag gccgttgcgg tcgggatggt ttatgaggcc gacctggcca
     2281 ggcacctcgg ctattgtggc gcagaggtgg ttgctcgatt gcgcgcactg gtgaaggccg
     2341 cgggtttgcc ggaccggttg ccgaaggtcg cgttcaatgc gttgtggggt gccatgcagc
     2401 aggacaagaa agtgtctgcc ggaacgattt tttgtgtggt gccggagcag atcggggccg
     2461 tgcgcattgt tccattggag aaggccacga cgcgcgcctg gtttcaggat gtccacacca
     2521 gagaggcagc cgaattgaag cgtgcaaggc ccgtcggacg ggcacgttag gagtggaatg
     2581 gcgactaaac gaacgctgac tcaagtcgag caagccttgc gtgaaaagac gcgtgaggtg
     2641 gatgtcctcc atcgcatcag cgagtcgatc agcaacacgc tcgacttgga ggccgtgctc
     2701 cggcatatcg tggatatggt cgtggaagtg accaaggccg acgcctgctt gctgtatttg
     2761 ttgtcggata acagggacga gctgatcctg cgggcctcca agaatccgca tccgaagctg
     2821 atcgggcgga tcaccatcgg catgggcgag ggcatcaccg gttgggtcgc gcgggagcgg
     2881 acgcgtgtcg tcattcccaa cagcgccagc gatgacacac ggtttaagtt ctttcacaac
     2941 cttccggaag accgctacca ggcgttcgtc tcggttccga ttacgaccaa gcaggaagtc
     3001 gtcggcgtca tcaacgtgca gcacaaacgg tcgcgccggt atcggcccga cgaattggcg
     3061 ctactctcga ccatcgccac gcaggtcgga ggggccattg agaacgcccg cctgtacgat
     3121 caaatgacgc ggaaggcgtt gcagctggat acgctgtccc aggtctcgga gaccgtgtcg
     3181 tcgaatcgat tgatcgagga cgtgctgcag ttgattgtga cgatgacggc tcaaatgatg
     3241 ggatcgaaga tttgctccat cattcttctg gatcagccga ccggtgaact tcggatcgcc
     3301 gccacacaaa gtctgagcga gcagtatcgg cgcaagccca acgtgaagat cgggcaaagc
     3361 atcagcgggc gtgccgttca ggatcgtcgg ccgatcatcg tgccggatgt catcaaagaa
     3421 ggcacctata tgtatccgga catggctgcg aaggagggcc tctgctcgct cctgtgcgtg
     3481 ccgatgctgg tgcgggagaa ggccatcggc gtgatcaata cgtatacgtc caagccgcat
     3541 accttcacgg cggaggatgt caaattgatg caggcgattg ccaatcaggc cgcgatttcc
     3601 attgagcaca ccacgttgct ggagaaatcg tttgaaatgc aggaagcctt gcaggtccgc
     3661 aagttgctcg accgggccaa gggatatctc atgcggtcga agcggctgtc ggaagaagac
     3721 gcgttcaaat tgattcaacg gcagagcatg gatcttcgca aatccatgcg agagatcgca
     3781 gaagccattc tcctggccgg agaaatcgaa gagcgggccg ataagggcag gagttgatcg
     3841 ccactaggcg aactttacga ccagaggagg gtgcaggccc tggcagttgg atcgatcttc
     3901 gcggtgctgg ggttgagccc gagccgttcc ttccagatta cttcacgctt cgtttgtctt
     3961 ctgctcctca ttctgcttca tcggcggtac tctcttggag ggctcagggg gtgagaccga
     4021 cttgtagccc gcctgacggt caagtgccac agtgaactga tttcgctgaa tgtctacaca
     4081 tcaatgaacg caacagtaca tgttcgagac tgtctgggaa taggtgaggt ttggatggcg
     4141 aagagccgac gcgttgccac agcttttctg ctaactctta tgacaagcgc ctgcgccacc
     4201 gggcaataca ctccgttagt cggggccgat cgcgatgagg tggagcagct caaggagaat
     4261 gtctatcgtg tggaataccg cgtcagttcg tttacctcgc aagaacaact ggatgcctac
     4321 ctgcgacggc gttgcgcgga gctgaccttg cgcgagggct acgatttctt ccacctcgcc
     4381 cagcgtgccg atgtgctggc actctcacga cgaacgtcga tgacggtgac gatgtacaag
     4441 ggcaagaagc cggtgggcgc tgatgacgtc tatgacgcga aggaggtctt ggcgcagccg
     4501 gacctccttt ccggcagcca atgaccgctg ccgttcgccg ttagggtgtt gccgtcggct
     4561 tgcggggcgc cgctttcggc ttgggcttgg acgcagcgac gtctttctca gctaactggc
     4621 gccgtaccga ttccaactct gccttgagct tctggagttc ctccgcctgt ttgaccacca
     4681 catccttcgt ggattgcagt tcctgctgca agcggctctg ttcggcagtg ggcgaggttt
     4741 gtgtcggtgc tgcctcggca gttgtgttgt ggtcggctgc gacaggggat ggcgcaggaa
     4801 gctgactcag cgtctcaagg gagacggcga tacgcggctg ttccggctcg acggtcatcc
     4861 agggttgagg ggcatcggct cgcacgaggg cttgtggggg cgtgaaggag agtatgagcg
     4921 acgcgggttt ggtctgtcca tccccggatg ttgatcggta gtgagacaga ttcactcgca
     4981 gcgtggtttt gtacacgtag agtgtgcctt gggtcgtgag accgtcatcc tgcatcgtga
     5041 agcgaacccg ctggtcaggt tgggc
//
LOCUS       DAOQRM010000174         5044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_17357_length_5044_cov_15.565043, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000174 DAOQRM010000000
VERSION     DAOQRM010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17357_length_5044_cov_15.565043"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..608
                     /locus_tag="PKK81_11465"
     CDS             165..608
                     /locus_tag="PKK81_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33547.1"
                     /translation="MAVARTLAFLCMIGISLTLPPRTTAGDQPQPSTTDRVIRDTKEA
                     VEATKQYTLQQKETFQKAVQAELAEMQVKIAELQKKTNAASAEARTEMQKALQELEHK
                     KAEAGKQLEEVNASTSSAWSKLKDNMNAAVEDLKKSYKETVSKLP"
     gene            718..1620
                     /locus_tag="PKK81_11470"
     CDS             718..1620
                     /locus_tag="PKK81_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HNV33548.1"
                     /translation="MPLKFALYPGCAAKGATPELYQSTMAIVGRLGIEVVELAASSCC
                     GAGVIGEADPDVALALNARTFAQAERLGLDIMTICGTCQGVMSAANRRLKNEPGTLER
                     VNTILAQDGISYGGGVQVKHLLWIAVRDIGLTRVGETVHSPFRNFRIAPFYGCYMLRP
                     SWELGFDDPENPSSLEKIIRAVGGEPVAYAGRTKCCGFPIILEKEAIAVAMAGTNMKD
                     AKDHGADFMVTPCPLCHMSLDIYQERAGQAVKTALNLPILHLPQLLGLAMGLPAQDLG
                     LSRHLISVDRIVTRLHTPDTTHSS"
     gene            1638..1958
                     /locus_tag="PKK81_11475"
     CDS             1638..1958
                     /locus_tag="PKK81_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV33549.1"
                     /translation="MKVLTLADFQQFSSEKMKKNNLFQTPRFFCDIYCFEPGQEQKGH
                     IHGEQDKVYIVLEGEGTFQVGSEKQVLRPGQGTMAPAGDEHGVKNHTQQRLKVLVFVA
                     PNQA"
     gene            complement(2029..2790)
                     /locus_tag="PKK81_11480"
     CDS             complement(2029..2790)
                     /locus_tag="PKK81_11480"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV33550.1"
                     /translation="MRTHAFCILAAAVLLVIAQPSMVGAVERAEAEETARLLAKLLQS
                     GRMVIERNQTLIDDPHKGEKGFTPELFEQQLVREFLAKTGIDLTALPAAPVSILIPPL
                     AKELLPALVQASREVVRDAQVVINQRGIGYKNFIPATYGSQASARFSKSAHVRLKQTA
                     IEPRNPKNEPDEYEASVLKWLSARPRAEAYVSELTEEGRMLRVVMPIYYAKDCLACHG
                     EPKGDLDISGYPKEGHKEGDLAGAITVTAPLTIRK"
     gene            complement(2813..3814)
                     /locus_tag="PKK81_11485"
     CDS             complement(2813..3814)
                     /locus_tag="PKK81_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNV33551.1"
                     /translation="MAVLCLCLPGCGLLIDAVEHVWPVSGNKVDIICERERVQVGLAM
                     EPFRPFVFPTIWTDEGARVTGLDVELVRAMTDELSRRCGRAVTPVLHLVRFRDLFRLL
                     NEGHLDWFMSAVAINTPAPARAGVAYSLPYFYGGGISGITTSPAVLERVRANVREQAI
                     HPTPDRLAATSHALDGLTIAVQDETSAYYYAEANLSPHRLILCDSLPAAFEYADSQAE
                     PRVDVILGAQPVLEYMVKRVRKDWSPLTRENGRPLFLTKADYGVVVAEESYHLRWLLN
                     DLLLKLDESGRLREMRTRWLDEEYAFPRRASLEGLSFDVEQMAAHYAQGTCRLKVEP"
     gene            complement(3844..4455)
                     /locus_tag="PKK81_11490"
     CDS             complement(3844..4455)
                     /locus_tag="PKK81_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33552.1"
                     /translation="MAHKWTGLLCGAIAIVGWCLASAASLSAQEAMLEFPIRPFNVDV
                     MLRQQTFAPDDREIGVQLGITALRYGNVEVRTIYQYFSIHSQEFRTDQHSLFLNPRWN
                     NFIDILDFPTHKPISRLLRHILFGPLEDRAVPYVGLLAGGVLPGPGNHAPGHLIGGQL
                     GVRFPVARSLSVDISVQYSQYGVDFRGEAGQAQQWVFLTGVRF"
     gene            complement(4535..4792)
                     /locus_tag="PKK81_11495"
     CDS             complement(4535..4792)
                     /locus_tag="PKK81_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33553.1"
                     /translation="MALFLSGQDCPSFRPSLAAELAQVPGVTRVEPDSVPDHVLIDVV
                     SGQVTSEDLLAAARRRLIPGMRCRIEIMQSCISAGPAPLPR"
BASE COUNT         1108 a         1556 c         1455 g          925 t
ORIGIN      
        1 ctcgccacgt gactcggcaa tttcgcgacg aaccgtcatg aataacgcgg gttaaccgtc
       61 cggcagggga tctgggggca cagaacatgc cggagaatga gtataatgtg accgcgttgg
      121 gccgaatggc tgcatcccgt ctgaaaacaa aaaggagtcc ccgcatggcc gtagctagaa
      181 cgctggcgtt tctgtgcatg atcggcatct ccctcacgct tcctccccgg acgaccgccg
      241 gagaccagcc gcaaccgtct acaaccgaca gggtgatccg ggataccaag gaggccgtcg
      301 aagcgaccaa acagtatacg cttcagcaaa aggagacctt ccagaaggcg gtgcaggcag
      361 aactggccga gatgcaggtc aaaattgcgg aactccaaaa gaagaccaac gccgcctccg
      421 cggaagcccg gactgaaatg cagaaggctc ttcaggagct ggaacacaaa aaagccgagg
      481 caggaaagca actcgaggag gtgaatgcct ccaccagctc agcctggagt aagctgaaag
      541 acaacatgaa cgccgccgtc gaagacctga agaaaagcta taaagagacc gtttcaaaac
      601 ttccctgatt ccacctatgg ctcacgcacc cgagagaatc ccgattccgt ctgttcatcg
      661 aggggtgaaa tacgctatgt tgggacggac agaccggacg aacaggcgac ccactctatg
      721 cccctgaaat ttgccctcta tcccggttgc gcagcgaaag gcgccacccc cgaactctat
      781 caatccacga tggccatcgt cgggcgatta ggcatcgagg tcgtcgagct tgccgcctcc
      841 tcctgttgcg gcgccggcgt gatcggagag gccgatccgg atgtggccct tgcgctgaac
      901 gcccggacct tcgctcaagc ggagcggctc gggctggaca tcatgaccat ctgcggcacc
      961 tgccaggggg tgatgagcgc ggcaaaccgc cgcctgaaga acgaaccggg cacccttgag
     1021 cgggtgaata caattctcgc gcaagacggc atcagctacg gcggcggggt ccaggtgaaa
     1081 catctactct ggatcgcggt gcgggatatc ggactgaccc gcgtgggtga gacggtccac
     1141 tctccatttc gtaattttcg tatcgcccct ttctacggat gttacatgct ccggccctcc
     1201 tgggagttgg gattcgacga tcctgagaat ccgtcatcgc tcgaaaaaat tattcgcgct
     1261 gtcggcgggg aaccggtcgc ctatgcgggc cggaccaagt gctgcggctt tcctatcatt
     1321 ctggagaagg aagcgatcgc ggtggccatg gccggcacca atatgaagga cgcgaaggac
     1381 catggcgcgg acttcatggt gactccctgt ccgctctgcc acatgagtct cgacatttac
     1441 caggagcggg cagggcaggc cgtcaaaacg gcgctgaacc ttcccatcct gcatcttccc
     1501 cagctacttg gcctcgcgat gggtctccct gcacaggact tggggctctc acgccacttg
     1561 atctccgtcg accgtatcgt gacgcgcctg cacacaccgg acacaacaca ttcatcctga
     1621 cccgtggagc ccacgacatg aaggtactca cgctcgcaga ttttcagcag ttcagttccg
     1681 agaaaatgaa gaagaataac ctcttccaga ccccgcgttt tttttgcgac atctattgct
     1741 ttgaaccggg ccaggaacag aagggccaca ttcacggcga gcaggataag gtctacattg
     1801 tgctggaagg ggaagggacg tttcaggtcg gctcagaaaa gcaggtgctg cgaccgggcc
     1861 agggcactat ggccccggcc ggcgatgagc atggagtgaa gaatcacacg caacagcggc
     1921 tgaaagtcct ggtgttcgtc gcgccgaatc aggcctgacc tccggcaacc gccatccggc
     1981 aatcagcgat cgttcctgtt atcagaatcc cgaccgacgc atgccgtctc attttcggat
     2041 cgttagcggg gccgtgactg tgatggcccc ggcaaggtcg ccttccttgt gcccttcttt
     2101 ggggtagccg gagatgtcga gatccccctt cggttcgccg tgacaggcga ggcaatcctt
     2161 ggcgtaatag atcggcatga cgacgcgcaa catacgcccc tcttccgtca gctcactgac
     2221 ataggcttcc gcgcgtggcc ttgcagagag ccatttcagc accgacgcct catattcatc
     2281 cggctcgttt ttcggattgc ggggctcgat ggccgtctgt ttcaggcgca cgtgcgccga
     2341 cttggagaag cgagccgagg cctgactccc atacgtcgcc gggatgaaat tcttatagcc
     2401 gatgccgcgc tggttgatga cgacttgagc atcccgcacc acttcccggc tggcctgcac
     2461 cagcgcaggc aagagttctt tcgccaacgg cgggataagg atggaaaccg gcgcggcggg
     2521 cagcgcggtg agatcgatcc cggtcttggc cagaaactcc cgcacgagtt gttgctcgaa
     2581 tagttccggc gtgaaccctt tctcaccctt gtggggatca tcgatcaacg tctgattccg
     2641 ttcgatcacc atgcggccgg attgcaggag ctttgccaac aaccgcgccg tttcttccgc
     2701 ctccgcccgt tcgaccgcac cgaccatcga cggttgcgcg atcactaaca ggactgcggc
     2761 cgccaggatg cagaacgcgt gagttctcat gaccattctc ctatccgtga tgttacggct
     2821 cgaccttgag gcgacaggtc ccttgcgcgt aatgggccgc catctgttcc acgtcaaacg
     2881 acaacccctc cagagaagcc cggcgtggaa aggcgtactc ctcatcaagc cacctggttc
     2941 gcatctcgcg aagtcgaccc gactcatcga gtttcaacag caggtcgttc aacaaccacc
     3001 gcaggtggta actctcttcg gccaccacaa ctccgtaatc ggctttggtc aaaaacaagg
     3061 ggcggccgtt ttcccgtgtc aacggcgacc agtctttacg aacccgtttg accatatact
     3121 ccagcaccgg ttgcgcaccg agaatcacgt cgacacgtgg ctctgcctgc gagtcggcat
     3181 actcaaacgc ggccgggagc gaatcgcata gaatcaaccg gtgggggctc agattcgcct
     3241 cggcgtaata gtaggcgctg gtttcgtcct gtactgcaat cgtcaatccg tccaacgcat
     3301 ggctggtcgc cgccagccgg tcgggagtgg gatgaatcgc ctgctctcgt acatttgcgc
     3361 ggactcgctc aagaaccgcg ggacttgtcg tgatcccgct gatcccaccg ccgtaaaagt
     3421 agggtaggga ataggccaca ccggcccggg ccggcgctgg tgtattgatc gccacggcgg
     3481 acataaacca atcgagatgc ccttcattca gcagtcggaa cagatccctg aaccgcacca
     3541 ggtgcaagac aggggtcacc gcgcgtccgc accgtcggga taattcatcg gtcatcgccc
     3601 gcaccagttc cacgtcgagc ccggtgaccc gagcgccctc gtcggtccag atcgtcggga
     3661 acacaaacgg acgaaagggc tccatcgcga ggccgacttg cacccgttca cgttcgcaga
     3721 tgatatcgac cttgttgcct gacacgggcc agacgtgctc gaccgcgtcg atcaacagcc
     3781 cgcacccggg aaggcacaga cagaggaccg ccatccatcc cacgcgaact cgcgacgccc
     3841 gcatcaaaac cgaacgcctg tgagaaatac ccattgttgc gcctgtcccg cctcgccgcg
     3901 aaaatctacg ccgtactggc tatattgcac gctgatatcg accgacagac tgcgtgccac
     3961 aggaaatcgc acgcccagct gacctccgat caaatggccc ggcgcgtggt tccccggacc
     4021 gggcagcaca cctccggcca ggagcccgac gtacgggacc gcccgatcct ccaagggacc
     4081 gaacaggata tgacggagca ggcgactgat cggtttgtgt gtgggaaaat ccaggatgtc
     4141 gatgaagttg ttccaacggg gattgagaaa cagggaatgc tggtcggtcc ggaattcctg
     4201 ggaatggatg ctgaagtatt ggtagatggt ccgaacctcc acgttgccgt agcggagcgc
     4261 cgtgatcccg agttgcacgc cgatctcgcg gtcgtcaggg gcgaacgtct gctggcgaag
     4321 catgacatcg acattgaagg gccggatggg aaattccagc atcgcctcct gggccgacag
     4381 agacgcggca gaggccagac accacccaac gattgcgatc gcgccacaca gcagaccggt
     4441 ccatttgtgc gccatgacgc cggtagtctc cgcagagagc ttcggataga ccgcgcctag
     4501 tctgtctgaa cggttcaaaa gtcgcaagcg ctgcttaccg gggcaacgga gcgggacctg
     4561 cggaaataca ggactgcatg atctcaatcc ggcaacgcat accgggaatc aggcgacgac
     4621 gcgcggccgc caagagatct tccgaagtga cctggccgct gaccacatcg atcagcacat
     4681 gatcagggac gctgtccggc tccacgcgcg tgacgccggg aacctgcgcc aactccgcag
     4741 cgagactcgg gcggaaagag ggacagtctt ggccggacaa gaagagcgcc acgcgctcgt
     4801 gcgaggtgtc ggcattgact gggctcgcta ggcatccgac gaacaggagc aagcagcacc
     4861 gcgtcatccc acgaaatggc atcggcatct cccaaaaacc acaagccttg gacaaattga
     4921 ttggtcatga tctttctttc ggactgtccg gccgaccctt tcgagccggc cgggtgaatc
     4981 aatccaacga ccggcgttat ttcgccggta attcccaggg caccatcggc atcaacggcg
     5041 ccgg
//
LOCUS       DAOQRM010000175         5021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_17466_length_5021_cov_14.288764, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000175 DAOQRM010000000
VERSION     DAOQRM010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17466_length_5021_cov_14.288764"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKK81_11500"
     CDS             complement(<1..332)
                     /locus_tag="PKK81_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNV33554.1"
                     /translation="MASQFVHLHLHTQYSLLDGANQIDPLMQQVKSFGQPAVAMTDHG
                     NMFGAVEFYRKAKEAGVKPIIGCEAYMAPGSRLEKNSHLAHNDYYHLILLATNLKGYQ
                     NLIKLVSKA"
     gene            749..3973
                     /locus_tag="PKK81_11505"
     CDS             749..3973
                     /locus_tag="PKK81_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33555.1"
                     /translation="MPGASREAKAPLTQFSGRRIVSMAEQKEEKSKLPLTGIVALLLA
                     VVSSLVIYQVPLKTSRPIDKEAEKAVSIGADRVQARLWQDPFEAVSTHRQKEAAVAKQ
                     PDSDAHHTFFKLLTAVTQAGAPSDFLLLPVFVDGSPYASGVESRLRDRYALVSALGAA
                     GYQPESGESIRYFVRPGNGHLVVPVEAFLGRPKPAEPKQSTQVLVLWLKEQDFSPKPL
                     LSLSELVTEVQQAFKQSTVSYRVVGPRSSGSLGAMVGELTALYGADQSSGGSSAASLA
                     ASRRQLDSLKGVQFYSSWATAADPVLLGELQGPDAARGVEQWFEAGGLRLIRTIGTDA
                     VLAEQLVEELKRRRIDLTIPRGEAGPCKEADSDSAAVSSRHSDGKCRIPHIALISEWD
                     TLYGRSLPRTFVAMARSLAETGPDRAMRGFEYHFNRLRSEQYPDWVHRYSYLAGLDGE
                     LPPKNTEKDSGGAQTKGKIGDGGLRGEQSLAETPAGRSQLDYLRRLVETLKREEASND
                     GEFKAIGVLGSDVYDKLMILQALRSDFPHALFFTTDLDARLTHPSQLQWTRNLVIASH
                     FGLALHPLIQTPIPPFRDSYQTALFYSVLRAVNDIVPGPGPGELQIPATVTFPKTVPP
                     RLYEVGRHGPVDISLDGPYDAGRRPEALPNLHLPRPDLDPDTGELRVLRPQTIALFLS
                     ALSLMALCALLINSELWQWAQSRWVQGAVVGLVLMVCLLLGLLCWAMSDRTAGEPFSL
                     TDGISVWPTTVLRLLAFLLCLLFLGHSWRRLRDNETQLSRRFRFFRPRAPLSIQAGSL
                     VGIHHWRPQPRGDAEAALLWREYRALGSWKERLVRIVPQALCYLGFGALLLVISGFPV
                     MPCRGSACFTISYAVLGFSVLAMTLLMFYVVDATRLCRRLIKIMVGTTIWWSDRLLVR
                     EAAKRGVDQAYVHEWIAVEFIAKRTAVISAMIYYPFVVVFLMAVARHSYFDRWDFPLG
                     LMAIFGVNAAYAFGNGVFLRRSAEQAKRAAVAQLKSRLDGLSGEVVFKKEKREQVERI
                     VALIEDYQEGAFLPFTRHPLFGAIALPTGGTGLVFLLEYLATIF"
     gene            4085..>5021
                     /locus_tag="PKK81_11510"
     CDS             4085..>5021
                     /locus_tag="PKK81_11510"
                     /inference="COORDINATES: protein motif:HMM:NF016100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33556.1"
                     /translation="MTKKSTGTRAQVGKSPASPGRNRTGTDAFMADLNRIMREQVFQS
                     LDDAQAFMTRLMEEGGGRLPLPRAQAPAERAQELIYQAWEARTQRQAVALAREALEIF
                     SDCADAYNVLAEAEARSPQEACTLYQQGVDAGRRSLGKAFFDEHRGYFWGMIETRPYM
                     RARRGLADCLWALGRKRESLTHCEALLELNPDDNQGIRHGLLSRYLTLGNDTGAARLF
                     RDYAHDASAAFLWSRVLLDLRRGDQVAAKEHLVLAMDGNPHVAGFFAGKRKPPARLPE
                     HYSPGDRNEAVLYIANFAEAWLASPDAMDWLTDQLA"
BASE COUNT          911 a         1371 c         1652 g         1087 t
ORIGIN      
        1 gctttgctca caagcttgat gaggttctgg tatcccttga ggtttgtcgc gagcagaatc
       61 aggtggtagt agtcgttgtg cgcgaggtga gaattcttct cgagcctact gcccggcgcc
      121 atgtaggctt cgcacccgat gatgggtttg acccccgctt ccttcgcttt ccggtagaac
      181 tccaccgccc cgaacatgtt gccatgatcg gtcatcgcga cggccggttg cccgaacgac
      241 ttcacctgtt gcatcagggg gtcgatctgg ttggcgccgt cgaggaggct gtattgggta
      301 tggagatgca gatgaacgaa ttgtgatgcc acgggcgaat tgtagtgaga gggaaagttg
      361 aagtcaaacg gaaggagggg ggagtgcggc ctggtagtct cctttgttcg cgcaacgcgc
      421 ggcctcagaa ggccctcgtt ggacgcgcac agtcggatac caccaagcca ccctccctgg
      481 atagaagagg agctggctcg gaaggccctc gcctgggtaa acggcgcggc gcaccggagg
      541 gttgggtggg taagaaggct gcgcgcagct ggaggctacc ctgactaccc tcctgggaaa
      601 taagggaagc gagctggagg gagcattgtg atgatgcgta tccaaggatg cagtgcgggt
      661 cttatcgagt caccaccatt tcctcattct ctccattgac agttttgttg cccaagacta
      721 gcctgtcgct gtttttaacg tgaagaagat gcctggcgcg agccgtgagg caaaggcgcc
      781 gctgacgcaa ttttccggaa gacggatcgt ctcgatggcc gaacagaagg aagagaaatc
      841 caagctgccg ctgacgggca tcgtggcctt gttgttggcg gtggtcagca gcctcgtcat
      901 ctaccaggta ccgctcaaga cgtcgcggcc aatcgacaag gaagcggaga aggctgtgtc
      961 tatcggggcg gatcgggtgc aggcgcgact ctggcaggat cccttcgagg ctgtctcgac
     1021 ccaccgacag aaagaggctg ctgtcgcaaa acagcctgat tcggacgcac accacacctt
     1081 tttcaaactg ctgacggcgg tgactcaagc cggagcacct tccgactttt tattgttgcc
     1141 ggtctttgtg gacggaagtc cctatgccag cggagtcgag tcgaggttgc gggatcgcta
     1201 cgccctcgtg tcggccttag gggcggcggg atatcagccg gagtccggcg aatccatcag
     1261 atattttgtc cgaccgggaa acgggcatct cgtcgtcccc gtcgaggcgt ttctcgggag
     1321 gcccaaaccg gctgagccga agcagtctac gcaggtattg gtgctttggc tgaaggaaca
     1381 ggactttagt ccgaagccgc ttctgtcgct cagtgagctg gtgacggaag ttcaacaggc
     1441 gttcaagcag agtacggtgt cctatcgtgt ggtggggcca cgctcctccg gttcgctggg
     1501 cgccatggtt ggcgaactga cggctttgta cggtgccgat caatcctcgg gcggatcctc
     1561 cgcggcttct cttgctgcct cccggcggca gttggattcg ctgaaagggg ttcaattcta
     1621 ttcatcctgg gcgacggcgg cagatccggt tctgttgggt gaacttcaag ggccggatgc
     1681 ggcacgcggc gtggagcagt ggttcgaggc gggcggactg cgcttgatcc ggaccatcgg
     1741 cacggatgcc gtgctggcgg agcaactggt ggaggagctc aaacgtcgcc ggatcgacct
     1801 gacgattccg cggggggagg ctgggccgtg caaggaggca gactccgatt ctgccgcggt
     1861 gtcatcccgg cactcggacg ggaagtgccg gattcctcat atcgctctga tctcggaatg
     1921 ggacacgctc tacgggaggt ccctgccgcg tacattcgtc gcgatggcac gtagcctggc
     1981 ggagacaggg cctgatcgcg cgatgagagg cttcgagtat cactttaatc gcttgcgcag
     2041 tgaacagtat ccggactggg tgcaccgcta ttcctacctg gccggcctcg acggcgagct
     2101 gccgcccaag aatactgaga aggattccgg tggcgctcag acgaaaggaa aaatcggaga
     2161 cgggggactg cgtggggagc agagtctggc ggagactccg gccggccgca gtcagttgga
     2221 ctatcttcga cggctggttg aaaccctcaa gcgcgaagaa gcgagtaacg acggcgagtt
     2281 caaggccatt ggcgtgttgg gcagcgacgt gtatgacaag ctgatgatcc tccaggcttt
     2341 gcggagcgac tttccccacg ccctgttttt cacgaccgat ctcgatgcac gattgaccca
     2401 tccgagccag ctccagtgga ccaggaatct ggtgatcgcg tcccatttcg gtctggcact
     2461 tcatccgttg attcagacac ccattccgcc gttccgcgac agttatcaga ccgcgttgtt
     2521 ctattcggtg ctgcgggccg tgaatgatat cgtgccgggc ccgggccctg gcgaactgca
     2581 gattccggcg acggtgacgt tcccgaagac ggtgcccccg cggttgtatg aagtcgggcg
     2641 tcatggtccc gtcgatatca gcctcgatgg accttacgat gccggtcggc gaccggaggc
     2701 gcttccgaac ctgcatcttc cgcgtcccga tctggacccg gataccggtg aattgcgcgt
     2761 gctccgcccg cagacgattg cgttgttctt atctgcactc agtctcatgg ccctctgtgc
     2821 gctgttgatc aacagcgagt tgtggcaatg ggcgcaatct cgttgggtcc agggagctgt
     2881 ggtcggcctt gtcttgatgg tgtgcctgtt gctcggcctg ctctgctggg cgatgagcga
     2941 tcgaacggcg ggtgaaccct tctcgctgac cgacgggatc agtgtctggc cgaccacggt
     3001 gctcaggctt ctcgcattcc tgctctgtct cctctttctc ggacattcgt ggagacgcct
     3061 gcgggacaac gaaacgcagc tgagccgccg tttccggttc ttccgtccac gggcgccgct
     3121 ctcgattcag gccggctcac tcgtcggcat tcaccactgg cgtccgcaac cgaggggcga
     3181 tgcggaggcg gctctgctgt ggcgggaata tcgggcgttg gggagctgga aggagcgact
     3241 tgtgcgcatc gtgccgcagg cactgtgtta tctggggttc ggggccctgt tgctggtgat
     3301 ctccggattt cccgtgatgc cttgccgcgg gtcggcctgc tttacgatca gctatgccgt
     3361 actggggttc agtgtcctgg ctatgacgct gttgatgttt tatgtcgtcg atgcgacgcg
     3421 gctctgccgg cgtttgatca aaatcatggt cggcacgact atttggtggt cggaccgcct
     3481 gctcgtccgg gaggctgcga agcggggagt ggaccaggcc tatgtacatg agtggatcgc
     3541 ggtggagttc attgccaagc gcacggcggt catcagcgcc atgatttatt atccgttcgt
     3601 ggtggtgttc ctcatggcgg ttgcccggca cagttatttc gaccggtggg actttccgct
     3661 cggtctcatg gcgatctttg gggtgaacgc cgcctatgcg ttcggcaacg gggtcttcct
     3721 gcgccgctct gctgaacagg ccaagcgcgc ggccgtcgcc caattgaagt cccggctcga
     3781 tggattgtcc ggcgaagtcg tcttcaagaa ggaaaaacgg gagcaggtcg agcgaatagt
     3841 ggcgctgatc gaggactatc aggagggcgc tttccttccg ttcacccgtc atccgttgtt
     3901 cggggcgatt gcgttgccca cgggcggcac cgggcttgta tttctgctgg agtacctggc
     3961 caccattttt tgatatgaac gtctccggcc ggtatgagcg cagagcacag atgccggcga
     4021 cgacgagggc tgaatcgatg ataggtgcga tctccaggag aggcgtgatc gggagcgtgc
     4081 ggtcatgacg aaaaaatcca caggcacacg cgcacaggtg ggaaagtctc ccgcatcgcc
     4141 ggggcggaac cggaccggca cggatgcgtt catggcggat cttaaccgaa tcatgcggga
     4201 acaagtgttt caaagtctgg acgatgcgca ggccttcatg actcgtctga tggaagaggg
     4261 gggtggccgg cttcccctgc ctcgggcgca ggctccggca gaacgggctc aagaattgat
     4321 ctatcaggcc tgggaggcgc ggactcaacg acaagctgtt gcccttgccc gcgaggctct
     4381 cgaaattttc tctgattgtg cggatgccta caatgtgctg gcggaggcag aggcacgatc
     4441 cccgcaggaa gcctgtacct tgtatcagca aggggtcgat gccgggcggc ggagcctggg
     4501 caaagccttt tttgacgagc acaggggata cttctggggc atgatcgaaa ccagaccgta
     4561 catgcgcgcg cgccgggggc tggccgattg tttgtgggct ctaggccgga agcgagaatc
     4621 gctcacgcat tgcgaagcgt tattagagct gaatcctgac gacaaccagg ggattcgcca
     4681 tggattgttg agtcgctatc ttacgctggg gaacgatact ggagcagctc gactctttcg
     4741 tgactatgcg cacgacgcct ctgccgcgtt tctgtggagc cgtgtcttgc tcgaccttcg
     4801 acggggagac caggtcgcag caaaggagca cttggtactg gcgatggacg gcaatccgca
     4861 cgtggctggg ttctttgccg gcaagcggaa accgccggct cgattgccgg aacactatag
     4921 tcccggagac cggaacgaag cggttttgta tattgcgaac tttgccgaag cctggttggc
     4981 gtcgcctgat gcgatggatt ggctgacgga tcagttggcg a
//
LOCUS       DAOQRM010000176         4937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_17890_length_4937_cov_17.832651, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000176 DAOQRM010000000
VERSION     DAOQRM010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17890_length_4937_cov_17.832651"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKK81_11515"
     CDS             <1..585
                     /locus_tag="PKK81_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNV33557.1"
                     /translation="RTHSTAIMTPDLLAALAQVESAGNPVARTYWRWRLTWNPLELYR
                     PASSAVGMFQITDATFQEGKRYCIHDHRVVQEGPWNDAHSCWFNSFYSRVLPSHAIEL
                     TSALLDRAVANAIGSHRRPRPTFQQKQDLAALIHLCGAGAGHAYVARSFRLAPQQRCG
                     DHSAKLYLNRVNELKRKFARLAAGESLLRFVRPQ"
     gene            842..1489
                     /locus_tag="PKK81_11520"
     CDS             842..1489
                     /locus_tag="PKK81_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33558.1"
                     /translation="MIEVDFRQASLPNGDEISGAMLVLDNLDHLPGETIDLLLQQVST
                     ARPRNMILTGGHRLRALLANPSTLPGLTIRLYPLSVLLDGELRRLVGHDMAAPIAKWT
                     GNHPYLSKLFLHYGETALAEGRQQWQPFLRQLIEEVGKGAERRLLNYLIEYGKPVNPT
                     KAGAETGTEDIKAVADRLVYLGAISRWIRNDEATLFAGCRLLNDFVTGGQSDHAD"
     gene            1791..2465
                     /locus_tag="PKK81_11525"
     CDS             1791..2465
                     /locus_tag="PKK81_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV33559.1"
                     /translation="MPVGRLTDLETGQSACIESLQAHYGVCFESRYGRHTSLANYAYL
                     DLLDQAWAAADRPVPTGGLVTDVGCANFWYAQTLHRFFRPAKLTGVDVEGYRLYPTGY
                     SRYDAAAGYVADLPQTSFVTVDYCEMDEQVDVITAWFPFVTPTPVLAWRLPLTVFSPE
                     RLFASIARNLAAGGTLFMVNQGTDEADVAAGYCRQAGLRSQGCWVHPRPLRPRPHPPV
                     ASWWTT"
     gene            complement(2482..2793)
                     /locus_tag="PKK81_11530"
     CDS             complement(2482..2793)
                     /locus_tag="PKK81_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33560.1"
                     /translation="MRSVMSITLIWMGMVAPVLGFQTAAEFPVSWVEHVDLRNHLLTF
                     KTEDGQVRMFRVADSVAMRREWLSKGDLVSIEVDLDDQIVNIVKVDRRSEPNRQVSRR
                     K"
     gene            complement(3055..>4937)
                     /locus_tag="PKK81_11535"
     CDS             complement(3055..>4937)
                     /locus_tag="PKK81_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HNV33561.1"
                     /translation="DTPMGANLIAGGATFRCWAPHAKSVHVVGDFNHQGRNEASLLTR
                     DAQGHWRGFIPGVKDRQRYMFYVVGEGSEGLKRDPYARELESPFPSQCIIRHPDFPWH
                     ECGYVTPRFHEYVIYQLHVGTFFTPNLPQKGGTFLDVARKLPYLAELGVNALQLMPIQ
                     EFQTSFSLGYNGTDYFSPEMDFAVADADLAPYVATVNGLLDAKGLRRYQVKELRGEMN
                     QLKALIDLAHIYGLAVLLDVVYNHAGGDFGDQSLYFFDRQDPAGGQRHSLYFTDVGHA
                     GGLVFDFAKPEVRDFLIQNAKFFLTEYRVDGFRYDQVSVIDHDGAPEGWRFCQDLTST
                     VHAQRPETLHHAEYWDVNPFIVAPRPVGAGFDTTLTDGLRMAIRDVIGNASAPDERPL
                     NMTALARSLWPEGFNESWRFVQGPENHDIVYRGREQRIARLGDPDHPRSWFARSRARV
                     ATGLSFTAPGIPMLFMGQEFLEDKQWADDFVTHRELLLHWAGLDQGDRQMIDHLRFTR
                     ELLHLRRRLPALRGQGFRVAHVHDQNRVLTFHRWVEGEGHDLLVVVHLADFTRVGYRL
                     GFPGEGDWRELFNSDAYENWINAGVAGNSGRVTAGLQPLHGFAYSAAVVLPANSMLIF
                     AR"
BASE COUNT         1002 a         1567 c         1406 g          962 t
ORIGIN      
        1 cgcacacatt ccactgccat catgacgccg gatctactgg ccgcgctggc gcaggtcgaa
       61 agcgcaggca atccggtcgc gcgtacctac tggcgctggc gactaacctg gaatccactg
      121 gaactgtatc gtccggcatc gagcgcggtc ggcatgtttc agatcaccga cgcgacgttt
      181 caggaaggaa agcggtactg cattcatgac caccgtgtcg tccaggaggg cccctggaac
      241 gatgctcact cctgctggtt caattccttt tacagccgcg tcctgccgag ccatgccatc
      301 gagttgacct ccgccctgct cgaccgcgcc gtggcgaacg ccatcggttc gcatcgaagg
      361 cctcgcccca cgttccagca gaagcaggat ctggccgccc tgattcatct ctgcggagcc
      421 ggcgcaggcc acgcgtacgt cgcccgtagc tttcgactgg ccccgcagca acggtgcggc
      481 gatcacagtg cgaagctcta tctcaatcga gtgaatgagt tgaagcggaa atttgcgcgg
      541 ctggccgcag gcgagagcct gctccggttt gtgcgccctc agtaacgagt ctcgaccgag
      601 attgccccga tcgcacgagc gatgagtcgt atcatggtca tgcgtggtaa agtggcctat
      661 gtccgtcgca cccttctttc cacagccggc caccggcgat gcgttcatcg gacgccaagg
      721 gcttgtccga aacctgcgcg gacgcctagc ggacggcgaa tccgtggcgg tgatcggctg
      781 accgaagctc ggcaagacgt cgctggtacg aagctcgctg gatgggctat cccaccaccg
      841 ggtgatcgaa gtggatttcc gccaagcatc actgccaaat ggagatgaga tatccggcgc
      901 aatgctcgtt ctggacaacc tcgaccatct gccaggcgaa acgatcgacc tgctgctcca
      961 gcaagtttcg actgcccgcc cgcggaacat gattctcacc ggcggccacc ggttgagagc
     1021 actgttggcc aacccttcaa cgttacccgg gttgaccatt cgcctctatc cactctctgt
     1081 cctgctcgat ggagaactcc gccggttggt cggccatgat atggcggcac ccatcgcgaa
     1141 gtggacgggc aaccatccgt acttgagcaa actcttcctg cattacggag aaacggctct
     1201 tgctgaaggc cgacagcaat ggcagccgtt ccttcgacag ctgatagagg aggtaggcaa
     1261 gggagcagaa cgacgattat tgaactatct gatcgagtac gggaaacctg tgaaccctac
     1321 gaaggcagga gctgagaccg gcaccgaaga tatcaaggcg gtcgcagacc gccttgtcta
     1381 cctgggagcc atcagccgct ggatcagaaa tgacgaggcc acgctttttg ccggttgtcg
     1441 gctgctcaat gacttcgtga ccggcggcca gtctgaccac gcagattaag cgcatgctgt
     1501 gccgaaccgc gaatgtttac agaacgaccg atgggatgat ggctagccat taccatccgc
     1561 cccccttcag ttcttcattg gaaaagccga catggtgatg aaacattctc cctatgccga
     1621 ccactcctcc agtcactttc catgctccac gcctggttca ggctcctgcc cccagtcctt
     1681 catcgcccaa ccatcgttat tctctcggac aacggtgccg gtcgctctat catgcactgt
     1741 ggttcggcct ctcggaacgg atacgctggt cgagaaagcc ttaccatgaa gtgccggtcg
     1801 gccgtctcac agatctggag accgggcagt cggcttgtat cgaatccctg caggcccact
     1861 atggtgtctg cttcgaatcc cgctacggac gacacacgtc cctggcgaac tacgcctacc
     1921 tcgatctgct ggaccaggcc tgggcggcag cggaccggcc cgttcccacg ggcggtcttg
     1981 tcacggatgt gggctgtgcc aacttctggt atgcgcagac gctgcaccgg tttttccgac
     2041 cggcgaaact gactggcgtg gatgtcgagg gttaccgcct ctatccaacc ggctacagcc
     2101 gctacgacgc tgccgccgga tacgtcgcgg atcttccgca gacctcgttt gtgaccgtcg
     2161 actactgcga gatggacgaa caggtagatg tcatcacggc ttggtttccg tttgtcacgc
     2221 ctacgcccgt tctagcctgg cggctcccgt taacggtgtt ttctccggaa cggctctttg
     2281 ccagcatcgc gcgcaatctt gcggcggggg ggaccctgtt catggtgaat caaggcacag
     2341 atgaagcgga tgtcgctgcc ggatactgcc gacaggcggg gcttcgttca caaggctgtt
     2401 gggttcatcc gcggccattg cgcccccggc cccatcctcc ggtcgcctcc tggtggacaa
     2461 cctagcagaa gctgcatcgc ctcattttcg acgtgacacc tgtcgattgg gttcggatct
     2521 ccgatccact ttcacgatgt tcacgatctg gtcgtcgaga tcgacttcga tgctgacgag
     2581 atccccttta ctcagccact cacgccgcat cgccaccgaa tcggccaccc ggaacatccg
     2641 cacctgaccg tcctcggtct tgaaggtcag taggtgattg cgaagatcca cgtgctcgac
     2701 ccagctcact ggaaactctg cggcggtctg aaatccgaga accggggcga ccattcccat
     2761 ccagatcagc gtgatagaca ttacactacg catgttcatc gtgacctcct ctcgattgca
     2821 ggtatgcggt cgaacatgaa cggcgtgtcg cgcacatcga gactctacgc cccatcgcac
     2881 cggcagtctg tcgctcctgc tgccattttg tagcggagat aagacctcag cctgcggtcg
     2941 tccgctctac gaagaactct tgtagcgcgc aaaggcgacg ggttcgttaa gaacaggccc
     3001 gtaggctcga gaaagagacc acatggagat gcggggaagg gaatccgggg gacactaacg
     3061 cgcaaatatc agcatactat tggcggggag caccaccgcg gccgaatagg caaagccatg
     3121 caggggttgc agaccggcgg tcacgcgccc gctgttgccg gccacgccgg cattgatcca
     3181 gttttcatag gcatcgctgt tgaacagttc gcgccagtca ccctcgcctg ggaatccgag
     3241 tcgatacccg acgcgggtga agtcggccag gtgcaccacc actaacaggt catgcccttc
     3301 gccttccacc cagcgatgaa acgtcaggac ccgattctga tcgtgcacat gcgccacgcg
     3361 aaacccttgt ccacgcagcg ccggcaatcg gcggcgtaaa tgcaacagtt cccgcgtgaa
     3421 ccgcagatgg tcgatcatct gccggtcgcc ctgatcgaga ccggcccaat ggagaagcaa
     3481 ctcgcggtgt gtcacgaagt cgtccgccca ctgcttgtcc tccagaaatt cctgtcccat
     3541 gaacagcatg ggaatcccgg gagcagtgaa gctcaggccg gtcgcgaccc gcgcacggct
     3601 gcgcgcaaac caggatcggg ggtggtccgg atcgccaagc cgggcgatgc gctgttcccg
     3661 tccccggtag acgatgtcgt ggttctccgg cccctgcaca aaccgccagg actcgttgaa
     3721 cccctcgggc cacaaacttc tcgctaacgc cgtcatattc agcggtcgct cgtcgggtgc
     3781 gctcgcgttg ccgatgacat cgcggatcgc catccgcagg ccgtcggtca acgtggtgtc
     3841 gaacccggcc ccgaccggcc gcggcgcgac gatgaacgga ttcacatccc aatattccgc
     3901 atggtgcaac gtctccggcc gctgtgcatg caccgtcgac gtcaggtcct ggcaaaaacg
     3961 ccagccctcg ggcgcgccgt cgtgatcgat cacgctcacc tggtcgtacc ggaatccatc
     4021 gacacggtat tcagtgagaa agaatttggc gttctgaatc aggaagtcgc gaacctcagg
     4081 cttggcaaag tcgaacacaa gaccgccggc atggcctaca tcggtgaagt acagtgagtg
     4141 acgctgaccg ccggccggat cctgccggtc gaagaaatac aggctctgat ccccgaagtc
     4201 tccgcccgca tggttgtaga ccacatcgag cagcaccgcc agaccataga tatgggcgag
     4261 atcgatcaac gccttgagct gattcatttc cccccgcagc tccttcacct gataccggcg
     4321 caatcctttt gcgtccagca gcccgttcac cgtcgcaaca tagggagcca gatcggcatc
     4381 cgccacggcg aaatccattt cgggagaaaa atagtcggtg ccgttgtagc ccaggctgaa
     4441 gctggtctga aactcctgaa tgggcatcaa ttgcagcgcg ttcacgccaa gctcagcgag
     4501 atagggcagc ttacgagcca catcgagaaa ggtgccgcct ttttgcggga gattcggggt
     4561 gaagaacgtc cccacatgga gctgatagat gacgtattcg tgaaaacgcg gcgtgacata
     4621 gccgcactcg tgccagggaa agtccgggtg acgaatgatg cattggctgg ggaagggcga
     4681 ctccaactcg cgggcatagg gatcccgctt gagcccctca ctcccctcgc cgaccacgta
     4741 gaacatgtag cgctggcgat ctttcacccc cgggatgaac ccacgccaat gcccctgcgc
     4801 gtcccgcgtc agcaaactcg cttcgttacg tccctgatgg ttgaaatcgc cgaccacgtg
     4861 gacggacttg gcatgcggag cccagcaacg gaacgtcgcg ccgccggcaa tcagattggc
     4921 ccccatcggg gtatcgg
//
LOCUS       DAOQRM010000177         4930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_17922_length_4930_cov_15.755487, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000177 DAOQRM010000000
VERSION     DAOQRM010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17922_length_4930_cov_15.755487"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /gene="serS"
                     /locus_tag="PKK81_11540"
     CDS             complement(<1..1115)
                     /gene="serS"
                     /locus_tag="PKK81_11540"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNV33562.1"
                     /translation="MYDLRVLRDNLDKLRDQLGARGKDVAWEDLRTLTEDRRTRTIQV
                     EELRHQLKKGSDDIARLKREKQPADEAMAAMKALGETIKGHEEQLRVVEEQLAHIALR
                     IPNLPHASVPDGRDEADNIEVRRWGTPPQLSAPAQSHWDLGEALGILDFDRAVRMAKA
                     RFAVLTGSGARLERALINYMLDMHTTQHGYREVLPPLLVNRTAMTGTGQLPKFEDDLF
                     QLQDEELFLIPTAEVPVTNLHREEILAEESLPIRYTAYTPCFRREAGSYGKDTRGLIR
                     LHQFNKVELVAFAKPDQSYDELERLTSHAEAILQGLGLHYRVITLCKGDMGFSAAKTY
                     DIEVWLPSQQTFREISSCSNFEAFQARRANIRCRAKAG"
     gene            1237..2253
                     /locus_tag="PKK81_11545"
     CDS             1237..2253
                     /locus_tag="PKK81_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNV33563.1"
                     /translation="MAVKLVSNPPNRFEPTQREALEPSSRVEIQLFEDDTHQILSRND
                     SPDLPFRWSVNPYRGCFHACAYCYARPSHEYWGFGAGTDFDSKIVLKRRAADLLKQAF
                     EKRSWDGELIVFSGNTDCYQPLESTLGLTRACLEICAAYRNPVGIITKGALPVRDLDL
                     FRQLQEQAWIRVYFSIAFADDETARQVEPQAPTVSKRFETMRILSEAGIPTGISVAPI
                     IPGLNEEAIPELLSRAKSAGSTSATCSLLRLPGHVETVFLDRMREAFPDRISKIIHRL
                     QEVRGGQLSEGGFFSRHHGNGTYWRCVEQLFDVGRRRAGFVEEDRPVPRTFRRPTAQQ
                     SLFD"
     gene            2264..2644
                     /locus_tag="PKK81_11550"
     CDS             2264..2644
                     /locus_tag="PKK81_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV33564.1"
                     /translation="MSHNPGFLKVVEEARGRVRECTVADVKARLDRAERFHFVDVRED
                     DEYAQDHAAGAIHLGKGVIERDIETVLPNKQEPIVLYCGGGYRSVLAADRIRLMGYTN
                     VISMDGGIKAWRAAGYPIEKGRRP"
     gene            2649..3719
                     /locus_tag="PKK81_11555"
     CDS             2649..3719
                     /locus_tag="PKK81_11555"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV33565.1"
                     /translation="MAFSRRELFNNAGFGLLLLPALMRSPRTILGHLLFDPDGPLVPL
                     KPIPENPYRRNGRSIVAIVYGKDTRRMLPRAVDLLGGLDLLGMRGKRVLLKPNVLNDQ
                     PPPSTTNPLVVAAMADMAKANGAAEVVVADGSGIIRLPTSANLTSTGMRAVAEAAGAR
                     VLALEDEPWVKIEVPQAQTLRQFYFSRPVYESDVVINMPVIKTHRFAEYSCSLKNFVG
                     AVHPRYRPSVRFWTGDWHERIAEINLAVHPALTVADGTTTMIEGGPTAGTPSKTDLVI
                     CSGDRVAVDLVAIALLRSFGTWPKLQGKRIGDQRQIKRAAALGLGVGSGQDIELVTEA
                     VDPAPPAFERLVEHIRRELRSV"
     gene            complement(3723..4280)
                     /locus_tag="PKK81_11560"
     CDS             complement(3723..4280)
                     /locus_tag="PKK81_11560"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNV33566.1"
                     /translation="MGVKWQGAGRRAAVVGVLVAATLGLFSTSPAAEFKMGVIDPQVV
                     LEKSKAGKRALDGLREYVATRQKLLSRDEEELRNTEKQIKEQASKLTDNEKKEKETSF
                     RAKIQDYQKRAQEFNQELQGKQKELVDEYMKKIAVATQAVADKAGYQLVLDKGSEQTV
                     KIVIFNKDTIDLTEQVIKEFDRTNK"
     gene            4482..4886
                     /locus_tag="PKK81_11565"
     CDS             4482..4886
                     /locus_tag="PKK81_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33567.1"
                     /translation="MRPSEQQQTHRLFVHALQHEAISCEHAGCPGPVETVDQSQTRDR
                     VKTFDLRCERCGWHTSLTGQEQLTPPWDNAALELMADEHLMHQQPVCPFDETPVVFIS
                     MPNPRRKAKYRVACFYCGRQIVMDWPPPESKR"
BASE COUNT          929 a         1439 c         1500 g         1062 t
ORIGIN      
        1 ccggcctttg cccggcagcg aatgtttgcc cgccgcgctt ggaacgcttc gaaattgctg
       61 caggaagaaa tttcgcgaaa cgtctgctgc gacggcaacc atacctcaat gtcgtaggtc
      121 ttcgctgcag aaaaccccat gtcccctttg cacagtgtga tcactcgata gtgcaatccc
      181 aacccctgca gaatggcctc ggcgtgcgaa gtcaatctct ccagctcatc ataggattgg
      241 tccggttttg cgaacgcgac gagctcgacc ttgttgaatt ggtggagccg gatgaggccg
      301 cgcgtgtcct tcccgtagga tcccgcttct cgtcgaaagc aaggggtata ggcggtatac
      361 cggatcggta acgattcctc ggccaggatc tcttcccggt gcagattggt gaccggcacc
      421 tccgcggtcg gaatcaggaa aagttcttca tcttgtagtt gaaacagatc gtcctcgaac
      481 ttgggcaatt gcccggtccc ggtcatggcc gtgcgattga ccagcagcgg cggcagcact
      541 tcccggtagc catgctgtgt ggtgtgcata tccagcatgt aattgatgag cgcccgctcg
      601 agtcgggctc ccgatccggt cagtacggca aagcgggctt tggccatgcg caccgcacgg
      661 tcgaaatcca gaatgccgag cgcttcgcca agatcccagt gggattgggc cggagccgag
      721 agctgcggcg gcgttcccca tcggcgcact tcaatattgt cagcctcatc ccggccgtcc
      781 gggacagagg cgtgagggag gttcggaatg cgcagcgcga tatgggccaa ctgttcttcc
      841 acgacacgaa gctgttcctc atggcctttg atcgtctctc ccaaggcctt catcgccgcc
      901 atggcctcat cggccggctg cttctctcgc ttcagccggg cgatgtcgtc cgatcccttt
      961 ttcagctggt gccgtagttc ttcgacctgt atggtgcggg ttcgacgatc ttccgtgagc
     1021 gttctcagat cttcccacgc gacatccttc ccgcgtgccc ccaattggtc acggagcttg
     1081 tccaggttgt cccgcaatac acgaaggtcg tacatagact ctcccctctg aagcggcgag
     1141 aaatctaaca ctggatgtcc gcgtagtcaa tgaaaccggt cgccaagcga ggccggggaa
     1201 aggtaattga caacgggtgg ggagggcggc accatagtgg ccgtgaagct tgtatccaac
     1261 ccacccaatc gctttgaacc cacgcagcgt gaggcgcttg aaccctcgtc gcgtgtggag
     1321 attcagcttt ttgaagacga cacccaccag atactgagcc ggaatgacag cccggacctt
     1381 ccgtttcgat ggagcgtgaa tccctatcgt ggctgtttcc atgcctgcgc ctactgctat
     1441 gccaggccat cccatgaata ctggggattc ggcgcaggca ccgacttcga ctctaagatc
     1501 gtactgaagc gccgtgctgc ggatctcctc aagcaggcgt ttgagaagcg atcctgggac
     1561 ggtgagttga ttgtgttttc cggcaacact gattgttatc aacccctcga gtccaccctt
     1621 ggcctgacca gggcttgcct tgaaatctgt gccgcatacc ggaatcctgt cgggatcatc
     1681 accaagggtg cgcttccggt tcgcgatctt gatctgttcc ggcaactgca ggaacaggct
     1741 tggatccgtg tgtatttcag catcgccttt gccgacgacg agacggcccg tcaggtcgaa
     1801 ccgcaggcgc cgaccgtcag taaacgcttt gagacgatgc gcattctctc agaggccggt
     1861 attcccaccg gcatatctgt ggcgccgatc attccaggct tgaatgaaga ggccatccct
     1921 gagctgttgt cgcgtgccaa gtcggcggga tcgacctcgg cgacctgcag tttgctgcgt
     1981 ttgccggggc atgtagaaac ggtatttctc gatcgcatgc gcgaggcgtt tcccgaccgg
     2041 atctcgaaaa tcatccaccg acttcaagag gtgcgcgggg ggcagctgag cgagggcggg
     2101 ttcttcagcc ggcatcatgg caacgggacc tactggcgtt gtgtcgaaca gttgtttgac
     2161 gtgggccgcc gtcgcgccgg attcgtcgag gaggaccgtc cggttcccag aaccttccgc
     2221 cgaccgacag cgcagcaatc attgttcgat taggaggaga gtcgtgagtc ataacccggg
     2281 gtttttgaag gtcgtggagg aggcgcgggg gcgtgtgcgc gaatgtaccg tcgccgatgt
     2341 gaaggccagg ctggatcgcg cggaacggtt tcattttgtg gacgtccgcg aagatgatga
     2401 atatgcgcag gaccatgcgg cgggggcgat tcacctcggc aagggtgtca tcgagcggga
     2461 cattgaaacg gtgctgccga ataagcagga gccgatcgtg ttgtattgcg ggggcgggta
     2521 ccggtcggtg ctggctgcgg acaggatacg cctcatgggc tacaccaatg tcatctcgat
     2581 ggacggggga atcaaggcct ggcgcgcggc cgggtacccc atcgagaagg gccggcgtcc
     2641 gtaatcccat ggcgttttcc agacgcgaat tgttcaataa cgcggggttc ggtctcctgt
     2701 tgctaccggc cctcatgcgc tcccccagga ccattctcgg ccatctgctg ttcgatccgg
     2761 acggccctct cgtccccctc aaaccaatcc ccgaaaatcc gtatcggcgg aacggccggt
     2821 cgatcgtcgc gatcgtgtac ggcaaggata cccgtcgtat gcttccacgc gccgtcgatc
     2881 tgctgggagg acttgattta ttggggatgc gcgggaaacg cgtgctgctg aagcccaatg
     2941 tgttgaacga ccagccgcct ccttcgacca ctaatccgct ggtggtcgca gccatggcgg
     3001 acatggccaa agcgaacgga gccgccgaag tggtggtggc cgatggatcg ggaatcatcc
     3061 ggcttcccac gtccgccaac ctgacgagta cgggcatgcg agccgtggcg gaagcggccg
     3121 gggcccgcgt cctggcgctg gaagatgaac cctgggtgaa gatcgaggtc cctcaggctc
     3181 agaccctccg gcagttctat ttttcccggc cggtctacga gtccgatgtg gtgatcaata
     3241 tgccggtcat caagacccat cggtttgcag agtattcctg tagcctcaag aattttgtcg
     3301 gggcggttca tccgcggtat cgcccatctg tgaggttctg gaccggtgac tggcacgagc
     3361 gcattgccga gatcaatttg gccgtgcacc ccgcgctgac cgtggccgac gggacgacga
     3421 ccatgatcga aggcggtccg acagccggca ccccgtcgaa aacggatctg gtgatctgca
     3481 gcggtgaccg ggtcgcggta gacctggtgg ccatcgccct gttgcggtcc ttcggtacct
     3541 ggccgaagtt gcagggcaag cgaatcgggg atcagcggca aatcaagcgc gcggcggcgt
     3601 tgggactggg ggtcggatcg gggcaggaca ttgagcttgt tacggaggcg gtggacccgg
     3661 ctccaccagc gtttgaacgg ctggtggagc atattcggcg ggagctgcgt agcgtgtaag
     3721 tattatttgt tcgtacggtc gaattccttg atgacctgct cggtcaaatc gatggtgtct
     3781 ttgttgaaga tcacgatctt cacggtctgc tcactgccct tgtccagcac cagctggtac
     3841 ccagctttat cggcgacggc ctgggtggcg acggcaatct tcttcatata ttcgtcgacg
     3901 agttccttct gcttgccctg cagttcctga ttaaactcct gggcgcgctt ctggtaatcc
     3961 tgaattttgg cgcggaagga ggtttccttc tctttctttt cgttgtcggt cagcttggag
     4021 gcctgctcct tgatctgctt ttcagtatta cgtaactctt cctcatcccg ggacaggagc
     4081 ttctggcgtg tcgccacata ctcccgcaac ccgtccagcg cccgcttacc ggctttgctt
     4141 ttttccagaa ccacttgagg atcgatgacc cccattttaa attccgccgc cggactcgtc
     4201 gagaacaggc cgagggtcgc tgcgacgaga actccgacca ctgccgcgcg ccggcctgct
     4261 ccttgccact tcacacccat ctcgggactc ctttcgccga aaaaaatgtc tgctacaaac
     4321 ctcgggagaa tagccgcccg tcgtgatcgt gtcaaggagg gcgccgtcga agggacaggc
     4381 gaattgacag gagggatgcc ggtggctata agcctggcaa cgcccgccat cgttccgaca
     4441 tccgggtagg tgttcccacc gagcgggtga gaaaggatct catgcgacct tctgagcaac
     4501 agcagacgca tcgactcttc gtgcacgcac tccaacacga agccatcagt tgtgagcatg
     4561 ccggatgtcc gggaccggtc gaaacggtcg accaatccca gacgcgcgat cgggtgaaaa
     4621 ccttcgacct gcgctgtgag cggtgcggct ggcacacgag tctcacgggg caggagcaac
     4681 tcacccctcc ctgggacaac gccgcccttg agttgatggc cgacgaacat ctcatgcatc
     4741 agcagccggt ctgtccgttc gacgagacgc cggttgtgtt catctccatg ccgaatccgc
     4801 gccgcaaggc taaatatcgg gtggcctgtt tttattgcgg caggcagatc gtcatggact
     4861 ggcctccgcc tgagagtaag cggtaacgat tcagggggcc gacgagggtg aagaggcggg
     4921 ggaggcgcgg
//
LOCUS       DAOQRM010000178         4922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_17958_length_4922_cov_16.767619, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000178 DAOQRM010000000
VERSION     DAOQRM010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17958_length_4922_cov_16.767619"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4646)
                     /locus_tag="PKK81_11570"
     CDS             complement(<1..4646)
                     /locus_tag="PKK81_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNV33568.1"
                     /translation="MQANRIEQGTAERTASGGLKNIEAIYPLSPMQEGMLFHTLMNPG
                     TGIYLMQNRYYVEGEMDAALFRKAWEQVIARHSILRTSFVWKSQKRPLQAVHKEIEVP
                     LDVMDWQGKDRAEQMADLDALLQQELEQGFDFGKAPLMHLRLIRLTDRTYQFVHSFHH
                     ILLDEWCISPLLMDFLAHYEACAQGLVCRAEKPRPYRDYIAWLQKQDLDEANRFWRDY
                     LRDFSTPTPLAYDRPPQGLADQNDDAADHCVHLSPDLTARLTTLAQQHRVTPNTFVQG
                     AWAILLAYYSGERDVLFGVTVAGRPTQLLGVESVLGLFINTLPLRVSVSPEQPVLAWL
                     KDLLAENIRLREYEYTPLIQIQRCSEIPRGEALFHSLFVFENAPVDPALCEGRIMFRA
                     EEEQYRVHTNYPMTVMGWPGKELGLKLSYDRRLFDSETVLRMIGHLKRILEGLIMCPD
                     ARIGDLPLLDHEERAALLAHGTGQTEGPRDDRSFATRFEAQVRRNPQAVAVTCGDRSL
                     TYEALNRRANRLADRLRTAGIDPDSIVALLDERGPDLLTMIVGCFKAGAAYLPLDPHH
                     PVARIARLLELSRVAVVVTSHRLKAHLDQALSVMPAASRPLVLTLDDLWSEQGCDDDR
                     PVPSRMDQLAYVIYTSGSTGVPKGAMVTLGGMLNNMESKLTSLSIGPTDVIAQTASQC
                     FDISVWQFLTALLCGARTHIVSDELVREPSRLLGHLDEAGVTIYEAVPAVLQALLDAG
                     DRSESQRRFSHLRWVLPTGEALPPALCRRWFERHPDIPLMNAYGPAECADDVAVHPIL
                     APPPEDAVRVPIGRAIQHLRLYILNRFFAPVPVGVSGELCIGGVGVGRGYVEDRLRTA
                     QAFVPDPFSNEPGARLYRTGDLARYRLDGTIEYVGRMDHQVKVRGFRIELGEIESCLT
                     DQDEVREAAVIVREDRPGERRLAAYVVPQDGQSIDSERLRTALQTQLPEYMVPSIFLT
                     LDALPRTPNGKVDRKALPAPDLDGAGGSTYVAPRSVTEELLAGIWADILGLERVGVRD
                     HFFELGGHSLLATQVVSRIRSAFQIELPLRAAFECPTVADLAATVDRVRAEGNGRQVP
                     ALTAGRREGPAPLSFAQQRLWFLAQLEPESWLYNLSFGLRLRGPLDVDALRRSFEAVA
                     MRHDQLRTRFLRAEGRPVQVTAESPTLPFERQLADPAPGVSWDQYVADLAADEARRPF
                     ALEGSLLWRVKLVQLSEQDHLLLVTLHHAIADGWSLNLLLSDMVAAYGALRADQPVPL
                     MTSTLQYADYAQWQRGWMTGDVLERELEYWKRTLAGAPSMLSLQPDHPRPIVQTFRGA
                     RHTFTVSQELTSTLQTLGRRQGTTLFMTLLAAFQVLLHRYSGEVDLSIGTPIANRTSL
                     ETEELIGFFVNTLVLRTDLSGNPRFVDLLERVRETVLGAQAHQDVPFEQVVDALKPER
                     DLSHAPLFQVMFALQTLGRQAMEIPDLTIEAVEVDPGSAKFDLSLEMTLAPEGLTGFF
                     EFNLDLFEPATIGRMADHFLTLLAGLAAEPQRKLVEFPLLASAERAQVVGAWNRT"
     gene            complement(4649..>4922)
                     /locus_tag="PKK81_11575"
     CDS             complement(4649..>4922)
                     /locus_tag="PKK81_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNV33569.1"
                     /translation="LAQVGRHDNFFELGGDSITSLQVLAKAHREGITLTPRQLFEHPT
                     IASAAAVAVIGAVEAMPPLPDEKQAERIVDVELSDEEMENLLKEIG"
BASE COUNT          936 a         1595 c         1538 g          853 t
ORIGIN      
        1 gtccggttcc acgcccccac cacctgcgcc cgctccgccg acgccagcag gggaaactcg
       61 actagcttcc gttgcggttc cgccgcgaga cctgcaagca gggtgaggaa atggtcggcc
      121 attcggccga tcgtggccgg ttcgaagaga tcgagattga attcgaaaaa gccggtgagt
      181 ccctccgggg ccagcgtcat ctccaacgac aggtcgaact tggcgctccc gggatcgacc
      241 tcgaccgcct cgatcgtgag gtccggaatc tccatcgcct gccgcccgag cgtctgcagg
      301 gcgaacatca cttgaaagag cggcgcatgg ctgagatcac gctcgggttt gagcgcatcg
      361 accacctgtt cgaacggcac gtcctgatgg gcctgagcgc ccagcaccgt ttcccgcacc
      421 cgctcgagga ggtcgacgaa ccgcggattg ccgctcaggt cggtacgcag caccagagtg
      481 ttgacgaaga agccgatcaa ctcctccgtc tcgaggctgg tgcgattcgc aatcggcgtc
      541 ccgatggaga gatccacctc gccgctgtag cgatgcagta gaacctggaa ggccgcaagg
      601 agggtcatga agagggtggt gccctgtcgg cggccgagtg tctgcagcgt ggaggtcagt
      661 tcctgggaga ccgtgaaggt gtgtcgcgcg ccgcggaagg tctggacgat gggacgcgga
      721 tggtcgggtt gcaggctcag catggagggc gcccccgcga gcgtccgttt ccaatattcc
      781 aactcgcgtt ccagcacatc gccggtcatc cacccgcgct gccattgcgc ataatcggcg
      841 tactgcaacg ttgaagtcat caacggaacg ggctgatccg cgcgcaaggc gccataagcc
      901 gcaaccatgt ccgacaacaa cagattcaac gaccagccgt cggcaatggc atggtgcagg
      961 gtgacgagca acagatggtc ctgctcgctc aattgcacca gcttcacacg ccataagagg
     1021 ctgccttcca aggcgaacgg ccgtcgagcc tcatctgcgg ccagatcggc gacgtactgg
     1081 tcccaagaaa ccccgggcgc cggatcagct aactgtcgct cgaatggaag cgttggagac
     1141 tccgctgtca cttgcaccgg gcggccctcg gcacggagaa accgcgtgcg caactggtca
     1201 tgtcgcatcg cgacggcttc gaaactgcga cgcaacgcgt ccacatcgag cggcccccgg
     1261 agtcggagtc cgaacgagag gttgtagagc cagctttccg gttccagctg cgccaggaac
     1321 cagagccgct gttgcgcaaa ggacaacgga gccggcccct ctcgacgccc ggccgtcaag
     1381 gcgggaacct gtctgccgtt tccctccgcg cgaacccgat ccaccgtcgc ggccaggtcg
     1441 gcgacggtcg gacattcgaa ggcggcccgc aacggaagct cgatctgaaa cgcactccgg
     1501 atgcgggaga ccacctgggt ggccaagagg gaatggcccc caagctcgaa gaaatggtcc
     1561 cgcaccccca cccgctccag gccgaggatg tccgcccaga tgccggccag caactcttcc
     1621 gtgacgctcc gaggcgccac gtaggttgat ccgccggcgc catccaggtc gggagcgggc
     1681 aaggccttcc ggtccacctt gccgttcggt gtccgcggca acgcatcgag cgtcaggaag
     1741 atcgacggca ccatgtactc ggggagctgt gtctgaagcg ccgtgcgcaa tcgttcgctg
     1801 tcgatcgact ggccgtcctg cggcacgaca tacgcggcca accgcctctc tcccggacga
     1861 tcttcgcgga cgatgaccgc cgcttcgcgg acctcgtctt gatcggtgag acaggattcg
     1921 atttcaccca gctcgatacg gaatccgcgc accttgacct gatggtccat gcgtcccacg
     1981 tattcgatcg tgccgtcgag acggtagcgg gccaggtcgc cggtgcggta gagcctcgcc
     2041 cccggttcat ttgagaaagg gtccggcacg aacgcctgcg ccgtgcggag ccgatcctcc
     2101 acatatccac gaccgacgcc gactccgccg atgcacagtt cgcccgagac gccaacaggt
     2161 accggcgcaa aaaaccgatt gaggatatag agccgcaggt gctggatggc ccgcccgatc
     2221 gggacccgga cagcatcctc gggcggcggg gccagaatgg gatgaacggc cacatcgtcg
     2281 gcacattccg ccgggccgta ggcattcatc aagggaatgt ccgggtggcg ctcaaaccag
     2341 cggcggcaga gggcgggcgg cagggcctcg cccgtcggca gcacccagcg caggtgtgag
     2401 aacctgcgct gggattcaga ccggtcgcct gcatccagca aggcctgcag gaccgcgggt
     2461 acggcttcat agatggtgac tccggcctcg tccaggtgcc cgagcaaccg tgacggctct
     2521 cgaaccaatt catcggacac gatgtgggtc cgagccccgc agagcagcgc ggtcaggaac
     2581 tgccacacgg aaatgtcgaa acattgcgat gcggtctgcg cgatcacgtc ggtcggcccg
     2641 atcgacaggg aggtcagctt gctctccatg ttgttgagca tgccgcccag cgtgaccatc
     2701 gcccccttcg ggacgccggt cgacccggag gtgtagatga cataggccag ctgatccatc
     2761 cgagagggta ccgggcgatc gtcgtcgcac ccctgctccg accacaggtc gtccaacgtc
     2821 agcaccagcg ggcgggacgc tgccggcatc accgacaacg cttggtcgag gtgggccttc
     2881 agccgatgcg acgtcacgac gacagccacg cggctcaact ccagcagccg cgcgatgcgc
     2941 gcgaccggat ggtgcggatc gaggggcaga taggcggcgc cggccttgaa acaacccacg
     3001 atcatggtca ggaggtccgg accccgctca tccagcaagg ccacgatcga gtccgggtcg
     3061 attccggccg tcctcaggcg atcggccagt cgattggccc ggcggttcaa cgcctcatat
     3121 gtcaacgacc ggtcgccgca ggtcaccgct acagcttgcg ggttccgcct cacctgcgct
     3181 tcgaaacgcg tcgcgaaact gcggtcgtcc cgaggtcctt ccgtctggcc ggtcccatgc
     3241 gccaacagcg ctgcccgttc ctcatgatcc agcaacggca ggtcgccgat gcgggcgtcc
     3301 ggacacatga tgagaccttc caggatgcgc ttgaggtgcc cgatcatgcg gaggaccgtc
     3361 tccgaatcga acaaccggcg gtcgtaggag agcttgagtc ccagctcctt gccaggccag
     3421 cccatgacgg tcatgggata attcgtatgg acccgatact gctcttcctc ggcccggaac
     3481 atgatccgtc cttcgcagag cgccggatcg accggcgcgt tctcgaacac gaacaggctg
     3541 tgaaagaggg cttctccgcg cggaatctcg ctgcagcgtt gaatctgaat gagcggcgta
     3601 tattcgtact cgcgaagccg gatgttctcc gccagcaaat ctttgagcca ggccagcacc
     3661 ggctgctccg gcgataccga gacccgcaga ggcagggtat tgatgaacaa ccccagcacc
     3721 gattcgacac ccaggagttg ggtcggacga ccggccaccg tgaccccgaa caacacgtcc
     3781 cgctctccgc tgtaatatgc gagcaggatc gcccaggccc cctgcacgaa ggtgttgggc
     3841 gtcacgcgat gttgttgtgc cagggttgtg agtctcgctg tcaggtcggg actcaagtga
     3901 acgcaatggt ccgccgcatc gtcgttttga tcggccaatc cttgcggcgg gcggtcgtag
     3961 gcgagcggcg tgggtgtgga gaagtcgcgc aggtagtcgc gccaaaaccg gttcgcttca
     4021 tccagatcct gtttttgcag ccaggcgata tagtcccgat agggccgggg cttctccgcc
     4081 cggcagacga ggccctgagc acaggcctcg tagtgggcca ggaaatccat cagcaagggc
     4141 gaaatgcacc actcgtcgag caggatgtgg tggaaactat gcacgaactg ataggtgcga
     4201 tcggtcaggc ggatcaaccg caggtgcatg agcggcgcct tgccgaagtc gaacccctgt
     4261 tccagttcct gctgcagcag ggcgtcaagg tcggccatct gctccgcgcg gtccttcccc
     4321 tgccagtcca tgacatcgag cggaacctcg atctccttgt gtacggcttg taagggacgc
     4381 ttctgacttt tccagacaaa ggaggtgcgc agaatcgaat gacgtgcgat gacctgttcc
     4441 caggctttcc gaaacagcgc ggcatccatc tccccttcta cgtagtaacg gttctgcatc
     4501 aggtagatgc cggtgccggg attcatcagc gtgtggaaca gcatgccctc ctgcatggga
     4561 gagagcggat aaatcgcctc gatgttcttg agcccgccgc tcgccgtgcg ttcggccgtt
     4621 ccctgctcga tcctgttcgc ctgcatgcct atccaatctc cttcaagagg ttctccatct
     4681 cctcgtcgct caattccacg tccacaatcc gctccgcctg cttttcatcc ggcaacgggg
     4741 gcatggcctc gacggctccg atcactgcga cggcggcggc cgacgcgatg gtcgggtgtt
     4801 cgaagagttg cctcggggtc agcgtgatgc cctcacgatg ggccttcgcg aggacctgca
     4861 aactcgtgat cgaatccccg ccgagttcga agaagttatc gtggcggccg acctgcgcga
     4921 gg
//
LOCUS       DAOQRM010000179         4848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_18349_length_4848_cov_18.756103, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000179 DAOQRM010000000
VERSION     DAOQRM010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18349_length_4848_cov_18.756103"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PKK81_11580"
     CDS             <1..1046
                     /locus_tag="PKK81_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNV33570.1"
                     /translation="TPQPIIDLRSDTVTKPSAAMRDAMARAEVGDDVYGEDPTINRLQ
                     EAGAAMVGKRAALFVPSGTAGNQLCLRAQTEPGQEVIVEGQSHIVRYELGAAAALAGV
                     QLHWVAGRRGLMTAEQVEAAIRPKNPYSIQSALICLENTHNAGGGTVYPLATIRAIHE
                     VASAHRLPMHLDGARLFNAVVASGVSAAEYAQHFDTVTFCLSKGLGAPAGSLIATDDR
                     LLLERIRRFRRMYGGAMRQAGILAAAGLYALEHNIQRLADDHAHARQLAARLQQIPGI
                     SIDPETVDTNILFFDVVGAKWSAKEFVAALKPHGLLLNAVGARSCRAVTHLDVPAEAI
                     EQAAEKIARVLDR"
     gene            1120..1347
                     /locus_tag="PKK81_11585"
     CDS             1120..1347
                     /locus_tag="PKK81_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33571.1"
                     /translation="MPLEVVSFQLISRILDVTDGLGLNREWVEIPLSPESPGQVRTLP
                     NGKLEIIVDADQPFEEWLGTLEQQIRRAQTT"
     gene            1365..2171
                     /locus_tag="PKK81_11590"
     CDS             1365..2171
                     /locus_tag="PKK81_11590"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNV33572.1"
                     /translation="MEPQLATSSPQSTPALPSSEELQAELLDIPEPPDMPPAVTPPGY
                     EDALAGAVKYVRAKRGGDLVGIILVGSGARRAVTAHSDIDLIALVKGPADGQEMIRVG
                     DRLVDIRYGEHKTVAEDLAYSPRLAPLLRKGRILFDHEETAAQLIAKAAQRFRQGPPP
                     AGIHERIRLKAECLHWLGKVEDLRDKPNTAQYLLQLFFEDCVTAFFRTRGLWFTAPVD
                     TLRFLASRDPALGELASQFLSAGTLHDRLTAGRRFADLLFRDIPLPPRVD"
     gene            2328..3221
                     /gene="gnd"
                     /locus_tag="PKK81_11595"
     CDS             2328..3221
                     /gene="gnd"
                     /locus_tag="PKK81_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HNV33573.1"
                     /translation="MELGFVGLGKMGMNMVTRLQQGRHRVVAYDRTPEVVKQAEGKGC
                     IGAASLSDLVAKLAAPRAVWIMVPSGAPTEETIQAIAALLQPGDTIIDGGNTRFHDDT
                     RRAVELKQRGLHYVDVGTSGGIWGLTVGYCLMIGGENEPVRRLTPIFQTLAPEQGWAH
                     MGTHGAGHYVKMVHNGIEYSMMQGYAEGFELMAKSDYRLDLGKIADVWMHGSVVRSWL
                     LELAVGALKQDPKLEQLKGYVQDSGEGRWMIQDAIDKDVPVPTLTAALFTRFRSRQEE
                     SFAEKMLAALRNAFGGHSVRR"
     gene            3249..4793
                     /gene="zwf"
                     /locus_tag="PKK81_11600"
     CDS             3249..4793
                     /gene="zwf"
                     /locus_tag="PKK81_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNV33574.1"
                     /translation="MSSPTTPVDIKPLPEAITPVEACTLVIFGGSGDLARRRLIPAVY
                     NLLLDGLLPDKYAVIGLGRKPMTDAEFRNLVREGVIAHSRQALVEDTWQAFERHLFYI
                     QGENEDAQTYSALRAKAEQIEQTMQLPGNRIFYLSIPPSSFASVCEGLAKSGLATPPA
                     GSAPYSRIIVEKPVGRDLASAQQINEVTGKVFDESQIFRIDHYLGKETVQNLMVVRFA
                     NSIFEPIWNHKYIDHVQITVSEAEGVGTRASYYEEAGALRDMIQNHLLQLLCLVAMEP
                     PYSLDPNVVRNAKMEVLRCLRPIVGKDVEQYTVRAQYAQGTAHGQPVPGYRREKGVSP
                     DSITETYVAVKAFVENWRWSGVPFYLRTGKALPKRASEVAVQFKDIPQILFNANPANP
                     QPANVLTLRIQPDEGLSLRIISRVPGTRAQTHPVEMDFQYSDVFGCPSPEAYERLLLD
                     VMAGDASRFMRRDAVEASWDWVTKILDGWAQQKLRWLPEYSAGTWGPVEGERMIQNDG
                     RAWRIL"
BASE COUNT          994 a         1544 c         1437 g          873 t
ORIGIN      
        1 acacacctca gcccatcatc gatctccgga gcgatacggt cacgaagcca tctgccgcca
       61 tgcgcgacgc catggcacgt gcggaagtcg gtgacgatgt gtacggggaa gacccgacga
      121 ttaaccggct gcaggaagcc ggagctgcga tggtcggcaa acgagccgcg ctcttcgttc
      181 catcaggcac cgccggaaac caactctgcc ttcgcgccca gacggaaccg ggacaggaag
      241 tcattgttga aggccagtct cacatcgtcc gctacgaact gggtgcggcg gcggcactgg
      301 ccggggtgca gctccattgg gtcgccggca ggcgaggact catgacggcg gaacaggtcg
      361 aggccgctat tcgccccaaa aatccataca gtattcagag cgcactcatc tgtctcgaga
      421 atacccacaa tgccggaggc ggcacagtgt acccactcgc gacgatccgg gccatccatg
      481 aagtggcttc cgcccacagg cttcccatgc acctggacgg ggcccggttg ttcaatgcgg
      541 tcgtggcctc gggagtgtcg gcagccgagt atgcgcagca ctttgacacg gtgacctttt
      601 gcctctcgaa gggactcggc gcgccggccg gctcattgat cgcaaccgac gatcgcctgc
      661 tactggagcg aatccgtcgg ttccgcagaa tgtatggagg ggccatgcgc caggccggga
      721 tcctggcggc agcaggactc tatgcattgg aacacaatat tcagcggctc gcggacgatc
      781 atgcccatgc gcgacaactc gcagcgcggc tgcaacagat tcccgggatc tcgatcgatc
      841 cggaaaccgt cgataccaac attctgttct tcgacgtggt cggtgcgaag tggtcggcga
      901 aggaatttgt tgcggcactc aaaccgcacg gcctgttgct caacgccgtc ggtgcccgca
      961 gctgtcgcgc cgtcacgcac ctcgacgtac cggccgaagc gatcgaacag gccgctgaga
     1021 aaatcgcacg tgtactcgat cgctagcccc tcctcacatt gacagtccgc ctgccccctc
     1081 ataaaatgcc tccaccgtaa atacaccaga gggggatgcg tgccgctcga agtcgtctcg
     1141 tttcaactga tcagccggat tctcgatgtg acagacggct tgggactcaa tcgcgagtgg
     1201 gtggaaattc ccctttcgcc ggaatcccca ggccaggtcc gcacgctccc gaatggaaaa
     1261 cttgaaatca tcgtcgatgc cgaccagccg tttgaagaat ggctcggcac cctcgagcaa
     1321 cagatacgac gcgcgcagac cacctgacac gtcgaaggat agacatggaa ccacaactcg
     1381 cgacctcctc ccctcagtcc accccggcgc ttccgtcctc tgaagagctg caggctgagt
     1441 tattggatat tcctgaacca cccgacatgc ccccggccgt gacgccgccc ggctacgagg
     1501 atgccctcgc cggtgccgtc aaatatgtcc gcgcgaaacg cggcggcgac ctggtgggca
     1561 ttattctcgt cggatcgggc gcccgacggg cggtgaccgc gcatagcgat atcgacctga
     1621 tcgccctggt caaaggcccg gccgacggac aggaaatgat tcgcgtggga gaccggctcg
     1681 tggacatccg ctatggcgag cacaagaccg tcgcggagga tctggcctat tctccccgcc
     1741 tcgcgccctt attgcgcaag gggcggatcc tgttcgacca cgaagagact gcggcacaac
     1801 tcatcgccaa ggccgcgcaa cggttccggc aggggccgcc tccggccggc attcatgaac
     1861 gcatccgcct caaggcggag tgtctgcact ggctgggcaa agtcgaggac ctccgcgata
     1921 aacccaacac ggcgcaatat ctcctgcaac tgttctttga agactgcgtg acggccttct
     1981 tccgcacccg gggactctgg ttcacggcac cggtcgatac gctccggttc ctggcctccc
     2041 gcgacccggc actcggggaa ctggccagcc agttcctcag cgcaggcacg ctacatgacc
     2101 gactgacggc cggccgacgc tttgccgatc tcctgtttcg cgacatcccc cttccgcctc
     2161 gcgtggattg acggggcccg tcgaggcgct cgaatgccgc catccgcttg catcggcatc
     2221 cggtcgtgct accatcggta ctccagagtg tatggacggc gactgaccgt ttgagactcg
     2281 cgcagcccca aataccacac accggcaacc accagagagg atgacctatg gaactcggat
     2341 ttgtcggact cggcaagatg ggcatgaaca tggtcacgcg cctgcaacag ggccggcatc
     2401 gcgtcgtggc ttacgatcgc acgcctgagg ttgtgaaaca ggcggagggc aaaggttgta
     2461 tcggcgctgc atcgctgagc gacctcgtgg caaaactcgc cgctcctcgc gccgtctgga
     2521 tcatggtccc gtcgggcgct ccgaccgagg agaccattca agccatcgcc gccctgctcc
     2581 aaccgggcga taccatcatc gacggaggca acacccgttt ccacgacgat acccggcgcg
     2641 ccgtcgaact gaaacagcga ggcctccact atgtcgatgt cggcaccagc ggcggaatct
     2701 gggggctgac ggtcggctat tgcctcatga tcggaggcga gaacgagccg gtccgacgat
     2761 tgacaccgat cttccagacg ttggcgcctg aacagggctg ggctcatatg ggaacacatg
     2821 gcgcgggcca ctacgtgaaa atggtgcaca acggaatcga gtacagcatg atgcagggct
     2881 acgccgaggg cttcgaactc atggcaaaaa gcgactatcg gctcgacctg gggaaaattg
     2941 ccgatgtgtg gatgcacggc agcgtcgtgc gatcctggct gttggagctg gccgtcggcg
     3001 ccctgaaaca ggatccgaag ctcgaacaat tgaaaggcta cgtgcaggat tccggcgagg
     3061 gacggtggat gattcaggat gcgatcgata aggacgtgcc ggtgccgacc ctcaccgcgg
     3121 cgctgttcac ccggttccgc tcgcgacagg aagagtcctt cgcggagaaa atgctggcgg
     3181 ctttgcgcaa cgccttcggc ggacacagcg tccgccgctg aggccttaac ccaacggacc
     3241 accacaccat gtcttctcca acgactcccg tggacatcaa gccactcccc gaagccatca
     3301 cgcccgttga agcctgcacg ctcgtcattt tcggcggttc cggagacctg gcccggcgac
     3361 ggctcatccc ggccgtctac aatctgctcc tcgatggttt gttgccggac aaatatgcgg
     3421 tgatcgggct gggccgcaaa ccgatgactg atgcggagtt tcgcaatctg gtgcgggagg
     3481 gggtcatcgc ccattcacgg caagccctgg tggaagacac ctggcaggca ttcgagcgcc
     3541 acctgtttta tatccaaggg gaaaacgaag acgcgcagac ctacagcgcg ctccgtgcca
     3601 aagctgagca gatcgaacag acgatgcagt tgcccggaaa ccgtattttt tatctgagta
     3661 tccctccgag ctcttttgct tccgtctgtg aggggttagc caagtctggc ctggcgaccc
     3721 cgcccgccgg ctcggcgccc tactcccgga tcatcgttga aaagcccgtc ggtcgagacc
     3781 tcgcttccgc tcaacagatc aacgaagtca ccggcaaggt cttcgacgag tcgcaaattt
     3841 tccgcatcga ccattatctg ggcaaagaga ccgtccagaa cctcatggtc gtccggttcg
     3901 ccaacagcat cttcgagccg atctggaacc acaaatatat cgaccacgtc caaattaccg
     3961 tcagcgaagc ggaaggcgtc gggaccaggg ccagttatta tgaagaggcc ggcgccctgc
     4021 gggacatgat tcaaaatcac ctcctgcaac tgctctgcct cgtcgcgatg gaaccaccct
     4081 actcgctcga tcccaacgtg gtgcgcaatg ccaaaatgga agtcctgcgc tgcctccggc
     4141 ccatcgtcgg caaggatgtc gaacagtata ccgtccgggc gcagtacgcg cagggaaccg
     4201 cgcatggcca gcccgtgccg ggataccgtc gtgagaaggg tgtgagcccc gactccatca
     4261 ccgaaacgta tgtggcggtg aaagccttcg tggaaaactg gcgctggtcc ggcgtgccgt
     4321 tttaccttcg caccggaaaa gccttgccga agcgcgcgag cgaagtcgcc gtccaattca
     4381 aagacattcc gcagattctc ttcaacgcga accctgcgaa tcctcagccg gcgaatgtcc
     4441 tgacgctgcg gatccagccg gatgaaggtc tctccctgcg catcatctca cgagtccccg
     4501 gcacgcgcgc gcagacgcat ccggtggaaa tggacttcca gtacagcgac gtcttcgggt
     4561 gcccgtctcc ggaggcctac gagcgcctgc tgctggatgt catggccggc gacgcctcac
     4621 ggttcatgcg ccgcgatgcc gtggaagcct catgggactg ggtgacgaaa atcctggatg
     4681 gttgggctca gcagaaactc cgctggttgc cggaatattc cgcgggaaca tgggggcctg
     4741 tggaaggcga acgaatgatt caaaacgacg ggcgcgcctg gcgtattctt taacccggat
     4801 tattcatgaa tgccgtcgcg aggcgtttga gaccgacgcc cgccgggg
//
LOCUS       DAOQRM010000180         4750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_18906_length_4750_cov_19.126731, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000180 DAOQRM010000000
VERSION     DAOQRM010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18906_length_4750_cov_19.126731"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="PKK81_11605"
     CDS             complement(<1..1637)
                     /locus_tag="PKK81_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV33575.1"
                     /translation="MAQDHSDNQSNLFQAVIGHLGLLFRLERRVLGLIVSYSIAIGLF
                     SLIVPLTVQELVNTFAFALQPITIVTLAAVMVAALLFVGAFRALQYYAVEVLERRIFA
                     RVAIAMAQQLPHLRYQGFKPRFANFFVETILMQRAVSVLLVDLINVIVGGAVGMTILV
                     FYHPYFLLYNAILLVGFNVVFFLMSHGGLKADMNLSHAKYDTLHWFQEIAYNLLHFKS
                     TDSQALLVKKTDQLLDTYIGIRRTRFGILIRQYLGSLGWQAIAHSGLIATAGWLLGIG
                     QLTLGQFVAAEVVVSGILSSFDGVVKRMGHIYYFLTALTELSFLFSLPKDQDTATLSI
                     PLPDPTVHGIRVTCKDLTFAPAGGPAVFERFNLEVTPGEKIGIYADSTMAKTALARVL
                     GGLESPTSGVIRYNGVDLRHLNLDSVNRFRGFILDSQLSLFEGTLEENIVLGREYIPY
                     SDVRWALRFTELEEEVDALPHGLKTHVRAAGKIFAPTHIVRILVARAILGRPQLLIFE
                     GILHNMHPAMRETILRRLCSKEEPWSVIFVSNDPNLTP"
     gene            complement(1772..3259)
                     /locus_tag="PKK81_11610"
     CDS             complement(1772..3259)
                     /locus_tag="PKK81_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV33576.1"
                     /translation="MKIHAWVLALAACGLCSLPDTGLSADETAKPRTLPPIPLSLNEV
                     HAWIDRSHPLLKGAGTEKTSARGKMLKALGAFEPVLVNDTEIERFIDKGTTKTQSVGF
                     NDTLVEARTPWGFRGSAGVRQSIGDAKIPDLGFGNGQQVILGGFLPLLKGLMINPENA
                     ELQRSELADPRADVKISQTRQDLFLAAATQFWDWVSAAKYVDVQRRALGVAEERLRQV
                     EGRAKAGAVAPLDVVEAGQEVQRRREIAIGAQRAVEQEQYKMSMFLWENQTPTAPQVE
                     RAPDFPPPTAVPTPEIVRADKLQAKTDRPEIREVDIEAKVNNIDLELAKNNLLPSLDL
                     EAAPARAPEKFVLGLGYRFGVELRMPILQRRSRGEVMEAQAQADRLVMVQKYREQQVV
                     VDVDNALSAIERAKERVVAAAESLRMAKTLEEGERFRFSLGATSVLFVNLRERNSVDS
                     EMQLVRAKADYQKALALYQWATGIWARSQPSATPVNYRVGASFSK"
     assembly_gap    3520..3619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3620..4182)
                     /locus_tag="PKK81_11615"
     CDS             complement(<3620..4182)
                     /locus_tag="PKK81_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNV33577.1"
                     /translation="MQRIREGLKSRIRSLVTQRTGLDEMAVDDLATSTQLQSWEAVQI
                     PERLRFSSRLAILLVGFFILIVAFVPWTQTITVTGQLSAYTPFERPQDVEAQITGRIR
                     KWHVFEGVRVKAGDVILELDDYDPNFMAPDLLSLLDQRKKALEDTRKAALSRADQLDK
                     RIKEMQNLVKAAVPSAGARVLEAESKVR"
     gene            4359..>4750
                     /gene="purE"
                     /locus_tag="PKK81_11620"
     CDS             4359..>4750
                     /gene="purE"
                     /locus_tag="PKK81_11620"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNV33578.1"
                     /translation="MSKNNTAVRASVGVLGGSKSDFPILEKAVAMLNELGIPNELLVV
                     SAHRTPDRLFAYAEQAIDRGIQVIIAGAGGAAHLPGMLAAKTSLPVIGVPIPTENLRG
                     LDSLLSIVQMPRGIPVATVAIGGAENAA"
BASE COUNT          916 a         1339 c         1455 g          940 t
ORIGIN      
        1 ggggtcaggt tgggatcgtt cgagacgaaa atgaccgacc acggctcttc cttgctgcag
       61 aggcgccgga gaatcgtctc gcgcatcgcg ggatgcatgt tgtggagaat cccctcgaaa
      121 atgagcagtt gcggccgccc caggatcgcc cgggcgacca ggatgcgcac aatgtgtgtc
      181 ggcgcgaaaa tttttccggc agcccggacg tgggtcttaa ggccgtgcgg cagagcatcg
      241 acctcctctt ccagctcggt aaagcgtaac gcccatcgca catcactgta gggaatatac
      301 tcccgaccga gtacgatgtt ctcttcgagc gtgccttcga acaacgacaa ctgcgaatcc
      361 aggatgaatc ctctgaaccg gttgacggag tccaggttga ggtgccggag gtcgacgccg
      421 ttgtagcgga tcacgccgga ggtgggcgat tccagaccgc ccagtacccg ggccagcgca
      481 gttttagcca tcgtgctgtc cgcataaatg ccgatttttt cgccgggcgt gacttccagg
      541 ttgaagcgct cgaagacggc tgggccgccg gcgggggcaa acgtcagatc tttgcaggtg
      601 acgcgaatgc cgtgcacggt cgggtccggg agcgggatgg agagggtcgc cgtatcctgg
      661 tctttcggga gcgagaataa gaagctcagc tccgtcaacg ccgtcaggaa gtaatagata
      721 tgtcccatcc gtttcacgac cccgtcgaag ctcgacagga tgccgcttac gacgacttcg
      781 gcggcgacaa attgtccgag cgtcaattgc ccgatgccca ggagccagcc ggccgtcgca
      841 atgagaccgc tatgcgcaat ggcctgccat cccagtgagc ccagatactg ccggatcaaa
      901 attccgaatc tggtccgacg gatgccgatg taggtatcca ggagctggtc cgtcttcttg
      961 accagcaggg cctggctgtc ggtcgacttg aaatggagca ggttgtaggc aatttcctga
     1021 aaccagtgca gggtgtcgta cttggcatga gagaggttca tgtcggcctt gaggccgcca
     1081 tggctcatga ggaagaagac cacgttgaat ccgaccagca ggatcgcgtt atacagcagg
     1141 aaatacgggt gataaaacac caggatggtc atgccaactg cgccgccgac gatgacgttg
     1201 atcagatcca ccagcaggac cgagaccgcc cgctgcatga gaatggtttc gacgaagaaa
     1261 tttgcaaagc gcggtttgaa gccctggtag cgcaggtgcg ggagttgctg agccatggca
     1321 atggcgaccc gggcgaaaat gcgacgctcc aacacttcga ccgcgtaata ctgcagggcc
     1381 cggaaggcgc cgacgaagag cagggcagcc accatgacgg cggccagggt gacgatggta
     1441 atgggttgaa gcgcgaacgc gaaggtattg acgagttcct gaaccgtgag gggcacgatc
     1501 agggagaaga gtccgatggc gatcgaatag gaaacgatga gtccgaggac gcgccgttcc
     1561 aggcggaaga ggaggccgag atggccgatg acggcctgga acagattgct ctgattatcg
     1621 gaatggtcct gggccatcgt gttactccag caaaacctga ccgggcccct tgccgtgtct
     1681 cttcgcgagc agtctgaagc cgtggaggtg acacgcctgt gccggaccgc ctcgtcggct
     1741 aaaacgtatc agagcagacc gtaaaatacc actatttgga gaagctcgcc ccgacgcggt
     1801 agttcaccgg tgtcgcggat ggctgactcc gtgcccagat gccggtagcc cattgataca
     1861 gggccagcgc cttctgatag tctgccttcg ctcgcaccag ctgcatttcc gaatcgacgg
     1921 agtttcgttc gcgcaggttc acaaacagga cgctggtagc gcccagactg aaacggaacc
     1981 gctcgccttc ctccagcgtc ttggccatgc ggagcgattc ggccgctgcg acgacccgct
     2041 ccttcgcgcg ttcgatggcc gagagcgcat tgtcgacgtc cacgaccact tgctgttccc
     2101 ggtatttctg caccatcacc aggcgatcgg cctgtgcctg cgcttccatc acttcaccgc
     2161 ggcttctccg ctgcaggatc ggcatgcgca actccacacc aaaccggtag ccgaggccga
     2221 ggacgaattt ctccggcgct cgcgccggcg ccgcttcaag atcgaggctg ggcagcaggt
     2281 tgtttttggc cagttccagg tcgatattgt tgaccttggc ttcgatatcg acttcgcgaa
     2341 tttccggccg atcggttttt gcctgcagtt tgtcggctcg aacgatttca ggggtcggca
     2401 cggcggtcgg gggaggaaaa tccggcgctc gctcgacttg cggcgccgtt ggcgtctggt
     2461 tttcccagag gaacatcgac atcttatatt gctcctgctc tacggctcgt tgtgcgccga
     2521 tggcgatctc gcgccggcgc tggacttcct gccctgcttc aacgacgtcg agcggggcga
     2581 cggcgccggc tttggctctg ccttcaacct gccgcaaccg ttcttccgcg actcccaggg
     2641 cccggcgctg cacgtccacg tactttgccg cagagaccca atcccagaac tgggtggcgg
     2701 ctgcgaggaa caggtcctgc ctggtttggg agattttgac atcggcgcgt ggatcagcca
     2761 attccgaccg ctggagttcg gcattctccg ggttgatcat gagtcctttg aggaggggca
     2821 ggaagccacc gagaatgacc tgttggccgt tgccgaaacc gaggtcggga atcttggcgt
     2881 caccaatgga ctgccgaacg cccgcgctgc cacggaaccc ccagggcgtg cgcgcttcga
     2941 ccagggtatc gttgaagccg acgctttgcg ttttagtggt ccctttatcg ataaatcgtt
     3001 cgatttctgt atcgttcacg aggacgggct cgaaggcgcc cagggctttg agcattttcc
     3061 cccgcgccga agttttttcg gtgccggctc ccttcaggag cggatgggac cgatcgatcc
     3121 aggcgtggac ttcgttcaac gagagaggaa tgggcgggag cgtgcgtggt ttcgccgtct
     3181 cgtccgcaga cagaccggtg tcaggcagcg agcagagccc acaggcggcg agggcaagta
     3241 cccaagcatg gatcttcatg aaacgcactc ctttctgagt ataaacaaga gagaacaagg
     3301 tcgaaggaaa tgagcgcttc ggcctggcgg ccggtctgaa ggggcctatt acttggcccc
     3361 gcgaccggct ttcggcaaga gcgtatcgat caagctcggg ggccgttcct ggtagtcggg
     3421 cggaaacaga ttgaagcggc gccacagctc gtaccagagc gggacacgat tgagaatgac
     3481 ccaccccatg gctttggtgc cctggcggac atgttcttgn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnc gcaccttgct ttccgcctcg aggacgcggg cacctgccga
     3661 agggacggcc gccttcacga ggttttgcat ttctttgatc cgcttatcga gctgatcggc
     3721 tcggctcaac gccgcctttc gggtgtcctc cagtgctttc ttgcgttggt cgagcaggct
     3781 caagagatcc ggggccatga agttcggatc gtagtcatcg agttcgagaa tcacatcgcc
     3841 ggccttgact cgcacgcctt cgaagacgtg ccattttctg atgcggccgg tgatctgtgc
     3901 ttcgacgtcc tgcggccgtt cgaacggtgt gtaggccgaa agttgtccgg tcacggtgat
     3961 ggtttgcgtc cagggtacga atgcgacgat gagtatgaag aagccgacca ggagaatggc
     4021 cagccgggag gagaagcgaa gccgctcggg aatctggacc gcctcccacg actggagctg
     4081 ggtcgatgtc gcaagatcat cgaccgccat ctcatccaga cccgtgcgct gggtgaccag
     4141 ggagcgaatt ctgcttttga gcccttcacg gatccgctgc acgtgtcccc tccagggcac
     4201 aatcagtgac acagaaattc acaacctatc atagtggatt gcggaggact tggtcaattc
     4261 aatgaggggt tccgccgggg tgcggcttga cccttcgacc ggagaacggc taaggttcgc
     4321 cgcatcgttc tttccttcag ccaaaggtgg cctctcacat gtcaaagaac aatacagcgg
     4381 tccgggcttc tgtcggggtg ctgggtggaa gcaagtcgga tttcccgatc ctggaaaagg
     4441 ccgtagccat gctgaacgaa ctcgggattc ccaacgagct cttggtggtc tccgcccacc
     4501 ggactcccga tcgtctcttc gcctacgcgg aacaggcgat cgaccggggc atccaggtta
     4561 tcattgcggg cgccggtggt gccgcccatc tgcctggaat gctggcggcg aagacttcct
     4621 tgccggtgat cggggttccc atccccacag agaatctcag aggattggac tcgctgttgt
     4681 ccatcgtgca gatgcccaga ggcattccgg tggcgacggt cgccatcggg ggcgcggaga
     4741 atgccgccat
//
LOCUS       DAOQRM010000181         4748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_18919_length_4748_cov_16.244407, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000181 DAOQRM010000000
VERSION     DAOQRM010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18919_length_4748_cov_16.244407"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1117
                     /locus_tag="PKK81_11625"
     CDS             122..1117
                     /locus_tag="PKK81_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV33579.1"
                     /translation="MEPASILIVEDEPVVAKDIQLSLQRLGYRVPATATSGEEAIRKA
                     SDMHPDLILMDIVLKGKMDGVETALQIQRKQDVPVIYLTAYADNHTLERAKVTSPAGY
                     MLKPYQANELRTTIELALHRAQHERHMRERLRWIATTMRCIGDGIVTTDRGGRVAYMN
                     PAAESLTGWSQDDAAGMGVTALLGFHEAGPGYESESPVQQAMTQVRIVAIEEALLISK
                     QGGRRTIRGSVAPVADDGGNVLGAVLVFHETASGERGLQASDRQGDTLWKMDAQLGRP
                     QGIINLCSWCKRVPDESGEWYDLATFIAERSAIQFNGGLCPECMDQCFPCDERHK"
     gene            1264..3279
                     /locus_tag="PKK81_11630"
     CDS             1264..3279
                     /locus_tag="PKK81_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV33580.1"
                     /translation="MANSTLTTPDSRPLRIALIGAGRHAQHHARAILRCPGVQLVAVA
                     DPSDAAQAAMRDIVPGIGCFKTPEELFASERLNVVHIITPPATHAPLARMAIKAGCHI
                     YVEKPFTESVEDAQQILDEASAKGLRVCAGHQLLYEPPTRVLTQYLPSIGRVVHVESY
                     FSFRTVRHAPGGRKVLRADHQLLDILPHPVYLLLQVLEQAGEGRTELLSLEVSQAGTV
                     HALVRRGGVTGTLIVTLEGRPVESYLRVVGKNGSLFADYVRSTTQRAIGPGSSGIDKL
                     FAPYRQAWQLLIGTTSAMANRFLKSQRSYPGLAELFTAFYESARTGGPSPLSPESLLE
                     TVRICERVAKALKVGEAKALAAAAPKPVESRGVLVTGGTGFLGKEIVRTLLSRGRPVR
                     VVARREPSPWERIAGAEYVVADVATGAAAQLFKGVDTVIHAAAETAGGWPEHQRNSLD
                     ATEQMVRGADAAGITHFVHVSSLAVLAQGNGRPIGDDHPLEPDSKGSGPYVWGKLESE
                     RLAVQLGKELGLSVKVIRPGALVDYRDFDPPGRLGKRLGNIFVAVGSPGDRLGVVDVG
                     FAGRFLGWMTDAWDNVPSPLNLLDPVSPTKQELLDRLRQANPDLTVVWLPRFVLVPLS
                     WLATLAQKILRPGKPAIDVAKVFSVLPYDTSGIAKLAPQVDHASEKR"
     gene            3520..>4748
                     /locus_tag="PKK81_11635"
     CDS             3520..>4748
                     /locus_tag="PKK81_11635"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV33581.1"
                     /translation="MNIGLNARALTIGLAFLGVVSGCAVDQRWRSAMQEGNHALRSGD
                     YTHAEESFVAARKEAEVLDPQGKRLAETLSQLGEVNRELGRFPRAEALFQEALAIRER
                     VYGPAHAETAASLTDLGELYRLQGLYTQSEALHQRAREIREQVFGADHSKTAESLNNI
                     AVVYQDQRRFADAEPVLQRALGILEKQLGPEHSTTAITRDNLAKMYQAQGQHARAMPL
                     YQRALTIHEKAFGPNHPIVARNLENLADTYRAQNQYPQAEVLYQRAVSIFKKSLGNDH
                     VDTAEAMSRLGQLYELQGLYSQAEPLFQQAIAIREKQQGAESPHVAGELKNLAGLYQS
                     QNKLEQSEDLYKQSLVIYEQSVGYDREAYVKALKSYASLLRRRQRSGEAERLEERAKA
                     VLKRLSLESQSQGKTAA"
BASE COUNT          944 a         1330 c         1530 g          944 t
ORIGIN      
        1 tggaacttgt gacgctgctg gcagagaagc tggacggcag cacggaactc cggagtggag
       61 cgggcacgga atggcagatt cgctttcagc aactgcaata ttcagaaagg gtgtgaacgc
      121 catggaacca gccagcatct tgatcgtgga agatgaaccc gttgtggcga aggacattca
      181 gctgagcctg caacggctgg ggtatcgcgt gccggctacg gcgacctccg gcgaagaggc
      241 gatccgcaag gcgagcgaca tgcatcctga cctgattttg atggatattg tgttgaaggg
      301 gaaaatggac ggagtggaaa ccgccctcca gattcagcga aaacaggatg tgccggtcat
      361 ctacctcacg gcctatgccg acaaccacac gctggagcgc gcgaaggtca cgtctccggc
      421 cggttatatg ctcaagccct atcaagcgaa cgaactccgg accacgattg agctggcgct
      481 gcatcgcgcc cagcatgagc ggcacatgcg cgagcgcttg cggtggattg ccacgaccat
      541 gcgttgcatc ggcgacggca tcgtgacgac cgatcgcggc gggcgagtgg cctatatgaa
      601 tcccgccgcc gagagcctga cggggtggag tcaggacgat gcggcaggca tgggcgtgac
      661 ggcgctcctg ggttttcacg aagccgggcc gggatatgaa tcggagagtc cggtacagca
      721 agcgatgacg caagtgcgca tagtggcgat cgaggaagcc ctgctgatct ccaagcaggg
      781 tggacgtcga accattcgcg gcagcgtcgc gccggtcgcc gacgatggag ggaacgtgct
      841 cggcgccgtg ctggtgtttc acgagaccgc atccggtgaa cggggcctcc aggcctctga
      901 taggcagggg gacacgctct ggaagatgga tgcgcagctc ggacgtccgc agggaattat
      961 caatttgtgt tcctggtgca agcgtgtgcc ggacgagtcc ggcgaatggt atgacctggc
     1021 cacgtttatc gccgagcgat cggcgataca gttcaacggc gggctctgcc cggaatgtat
     1081 ggatcaatgt tttccctgcg acgaaaggca taagtagttc aagatgtgtg tggacctgtt
     1141 cacacgaggt ccctcgcacg ttgcgcccac cgccgcagtc ttcctccctg cgctaatttt
     1201 cgagaaggct tcaagaatga gtgagaatag actatagtag cgctgcacac tcaaacagga
     1261 cttgtggcca actctactct gacaactcca gactcgcgcc cgttacggat cgcgctcatc
     1321 ggtgccggcc gtcatgccca gcaccatgcc cgcgctattt tgcgctgccc cggcgtgcaa
     1381 ctggtggcgg tggccgaccc gtccgacgcg gctcaagcgg cgatgcgcga tattgttcca
     1441 ggcatcggct gtttcaagac gccggaggag ctgtttgcct ccgaacgtct caatgtggtg
     1501 cacatcatta ctccgccggc cactcatgct ccgttggccc gcatggcgat caaggccgga
     1561 tgccacatct atgtagagaa gcccttcacc gagtcggtgg aggacgccca gcagatcctg
     1621 gatgaggcga gcgcaaaagg tcttcgtgtc tgtgctggcc atcagctgtt gtatgaaccg
     1681 cccacccggg tattaaccca gtacttgccg tcaattgggc gcgtcgtgca cgtcgagagc
     1741 tacttttcct tccggaccgt gcgtcatgcg ccgggtggcc gcaaggtgct acgcgccgac
     1801 caccagttac tcgatattct gccgcatccg gtttatctgt tgctccaggt gttggagcaa
     1861 gccggggagg gccgtaccga gctgttgtcc ttggaagtga gtcaggccgg gacggtgcat
     1921 gcgttggtgc gacgcggcgg cgtgaccggc acgttgatcg tgacgctcga agggcgcccg
     1981 gtggagagtt atctgcgggt cgtcgggaag aacgggtcgt tgttcgccga ctatgttcgc
     2041 agtaccactc agcgcgccat cggtcccggg tcgtccggca tcgataagtt attcgcgcct
     2101 taccggcagg cctggcaatt gctgatcggc acgacctccg cgatggcgaa ccgatttctc
     2161 aagagtcagc ggagttatcc cgggctggcc gaactgttta ccgcgttcta tgagtccgcg
     2221 cggacgggcg ggccgtcgcc cttgtcgccg gagagtttac tggagacggt gcgcatctgt
     2281 gagcgcgtgg cgaaggccct gaaggtcggc gaggccaagg ccctggctgc cgccgcgccg
     2341 aagccggtcg agagccgggg cgtgctggtc accggtggaa ccgggttttt gggaaaagaa
     2401 atcgtgcgaa cgctgctgtc gcgaggccgc cctgttcgtg tggtggcgcg acgtgagccg
     2461 tccccctggg agcggattgc cggcgccgaa tatgtggtgg ccgatgtggc cacgggagcc
     2521 gccgcacagc tcttcaaggg ggtggatacc gtcattcatg ctgcggcaga aaccgccggt
     2581 ggctggccgg agcatcagcg taactcgttg gatgccaccg agcagatggt tcgcggggcc
     2641 gatgcggcgg ggattacgca tttcgtccat gtcagcagtc ttgccgtatt ggcgcagggc
     2701 aacggccggc ccatcggcga cgatcatccg ttggagccgg acagcaaggg gtccggtccc
     2761 tatgtttggg gaaagttgga atccgaacgg ttggcggtcc aactcggcaa agaactcggt
     2821 ctatccgtga aagtgattcg tcccggggca ttggtcgact atcgcgattt cgacccgcct
     2881 ggccgccttg gtaagcggtt ggggaatatc ttcgtggctg tcggttcgcc gggcgatcgt
     2941 ctgggggtgg tagatgtcgg atttgccggc cgcttccttg gctggatgac ggacgcgtgg
     3001 gacaacgtgc cgagcccctt gaacctcctc gatcctgttt ccccgaccaa gcaggaactg
     3061 ttggatcgtc tccggcaagc caaccccgat ctcacggtgg tctggctccc gaggtttgtg
     3121 ttagttcccc tctcctggct ggcgacgctc gcgcagaaaa tcctgcgtcc cgggaagccg
     3181 gctatcgatg tcgccaaggt gttcagcgtc ctcccctacg atacctctgg gattgcgaag
     3241 cttgcccctc aggtcgatca cgcctccgag aaacgataac tgtctctccc gcatggtccg
     3301 gttccttatt aatcatcggc atggggccgt ttcatcctct gtggtcgaaa tgtgtgcatt
     3361 aggggaagtg gttgaaaaat atagcgatgc cgaagggatc gaaaaagttg ccctcgtggg
     3421 gttgatttga ggcgtcagag ggggtaagtc ttaggagtat tctacgagca aggccagtcc
     3481 ccaaacggca accgtctcac caccgaagag ttgatcatca tgaacattgg attgaatgcg
     3541 cgtgcgctta ccatcggtct ggcctttctg ggcgtcgtct caggatgcgc ggtagaccag
     3601 cgctggcgtt cagccatgca ggaaggtaat cacgccctgc gttcgggcga ctacacgcat
     3661 gcggaggaat cctttgtggc tgctcgcaag gaagcagagg tgctcgatcc acaagggaaa
     3721 cgtttggccg agacgttgag tcagctgggc gaagtgaatc gtgagttggg gcggtttcca
     3781 cgcgccgagg cactctttca ggaggccctg gcgatcaggg agcgcgtgta tgggccggcc
     3841 catgccgaga cggctgccag cctgaccgac ttgggcgaac tctaccgcct acagggactc
     3901 tacacgcagt ccgaagcgct tcatcaacgg gcacgggaaa tccgcgagca ggtgttcggc
     3961 gcggaccata gcaagacagc cgagagtttg aacaatattg ctgtggtcta tcaggatcaa
     4021 cgacgcttcg ccgacgccga gcctgtcttg cagcgcgcac tcgggattct tgaaaaacaa
     4081 ctcgggccgg aacacagcac gacggccatc acgcgcgaca acctggcgaa gatgtatcag
     4141 gcgcaggggc agcatgctcg cgccatgccg ttgtatcagc gcgccttaac gattcatgaa
     4201 aaggccttcg gtcccaacca tccgatcgtg gccaggaatc ttgagaatct tgcggatacg
     4261 taccgggccc aaaatcaata ccctcaagcc gaagtgctgt atcaacgcgc agtcagcatt
     4321 tttaaaaagt ctctcggcaa cgaccatgtc gataccgccg aggcgatgag tcgcttggga
     4381 caactctatg agttgcaggg actctactcc caggcggagc cgctctttca gcaggcgatt
     4441 gcaatccggg aaaagcagca gggcgcagag agccctcacg ttgcgggtga actcaagaat
     4501 ctggcgggac tctatcaatc gcagaataag ttggaacagt ccgaggatct ctataaacag
     4561 tcgctggtca tctacgaaca gtccgtcggg tatgatcgcg aggcctacgt gaaggccttg
     4621 aagagttatg cgtccctgtt gaggcgtcgg cagcggtccg gcgaagccga gcgtctggag
     4681 gagcgagcca aggcggtctt gaagcgtctc tcactggaga gtcagagcca agggaaaact
     4741 gctgcgga
//
LOCUS       DAOQRM010000182         4695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_19237_length_4695_cov_17.949784, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000182 DAOQRM010000000
VERSION     DAOQRM010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19237_length_4695_cov_17.949784"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKK81_11640"
     CDS             complement(<1..626)
                     /locus_tag="PKK81_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNV33582.1"
                     /translation="MAKRRGWRGSLLMGLMAVAALGFVGTAQAGKAELKGNFQVLSDE
                     KSTHKPGKVQLIEFADFYCPHCHRFDGEGLAILEKEFGNKLEAVMVGYPVIPGKLPTA
                     FDMYEQAKTMGKGAEMKRALFRTIHKDKIGIIDKAIREVLIREVGLDPAAFEEGLASA
                     KPAKAFEDGRKWGDRIKIQQTPTVLLDGNIKVEQIDPENLKLIIHSIL"
     gene            729..1274
                     /locus_tag="PKK81_11645"
     CDS             729..1274
                     /locus_tag="PKK81_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNV33583.1"
                     /translation="MKLLQGIGILWFAAILAACASSGDMHESADGGPHVPPFSQIKAA
                     PDSFKGQTLVLGGQVLAARRMKDGTRIEVLQLPLNDSQQPTLDLMKSQGRFVAVQRDF
                     LDPATIPQGTFLTITGELTGSMTLPLDETDYTYPVIDIKSFRTWIPSQDSTMSRIRPY
                     PYYSPFWHPYWGPYGRFPYYW"
     gene            complement(1384..1863)
                     /locus_tag="PKK81_11650"
     CDS             complement(1384..1863)
                     /locus_tag="PKK81_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33584.1"
                     /translation="MLSRIAVLFMGVLGVLASGTSALALQAGGVEVGDLETGSVVGQP
                     FKELVVDAELYAVEIGNMKAWYPPTTVIDFKVRPGRPLLIKVTNTTATERGFQMIDTV
                     NAASPFVLKAEVRLKPGETKYIGVPTSDLFNQTQGNTLEYRDQLNPKQPGGKLLMIK"
     gene            complement(1880..2224)
                     /locus_tag="PKK81_11655"
     CDS             complement(1880..2224)
                     /locus_tag="PKK81_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33585.1"
                     /translation="MRKLFVAVVQILVLAGGSGVWAADDSLERLLRAQAFNREFEGFD
                     SYYVKIEADQPQPDGSHEVLAVASGKFSDNTKRMKVLFLIVDGHIVGGQVLEGTGLPP
                     CLAQEHRPSSSL"
     gene            complement(2480..2785)
                     /gene="smbP"
                     /locus_tag="PKK81_11660"
     CDS             complement(2480..2785)
                     /gene="smbP"
                     /locus_tag="PKK81_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HNV33586.1"
                     /translation="MMTVLRSGMLAFAVSALVATAPVGYALADGGGHVKETIKHAKEG
                     VEHEKEAIKHLEESVKASNDPHAKEALEHAKEAVKHAEESLAHAEQASSHKGKAKGK"
     gene            complement(3008..3535)
                     /locus_tag="PKK81_11665"
     CDS             complement(3008..3535)
                     /locus_tag="PKK81_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33587.1"
                     /translation="MPVSAAVASPRRAEMAQGAQHRAGLSVAVVRLLAVALDISEGWS
                     PAPPAATLLIPFGYRDWPSVTSTVESPGGRKRLRFYVCPKALLTPDYESFPVGTTFVV
                     ESEPYPEGPGSYRARPSVFAMEKCAGLTMDGAGTRQYESWMYASWDSPAQQAMADRGR
                     CGVCRLPWLTPADVR"
     gene            complement(3567..>4695)
                     /locus_tag="PKK81_11670"
     CDS             complement(3567..>4695)
                     /locus_tag="PKK81_11670"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV33588.1"
                     /translation="RWLLMALVVGMALCGVDGSWANEPDAWKQLFTDGVKAREAAHYA
                     EAEQLLQRALRGAEQVSPDDPRVATAANNLGLLYHDQGRLQEAQSLYTRALAIWEQQL
                     GPEHEDVAAALNNLAEIAHAEGDWGRAEPLYERVLAIERKVLGAAHPDVAISLNNLAE
                     LYRKQGRETAAESMYHLALILMEEARGTDHPDLGPVLNNLAVLYKGRGLYAWAEPFYL
                     RALKMRRLRFGDEHPAVAMSLNNAACLYQAEGLYASAQRYFDEALAIAERTSGPQASL
                     TGTIVGNMAFLADEQGLWTQAQLLYQRALVVQQAQLGLHHPTVGLLVERYARVLDTLG
                     QPVEAGLFAARAASIRARVQPGVVRQGSAMRQGPAVGELTR"
BASE COUNT          919 a         1568 c         1269 g          939 t
ORIGIN      
        1 tccaggatgc tgtgaatgat cagcttcaaa ttctccggat cgatttgctc aaccttgatg
       61 ttgccgtcga gcaggaccgt gggcgtttgt tgaatcttga tgcgatcgcc ccatttccgc
      121 ccgtcttcga aggccttcgc cggcttggcg ctggccaacc cttcttcgaa cgccgccggg
      181 tcgagcccga cttcacgaat caacacttca cggatcgcct tgtcgatgat gccgatcttg
      241 tccttatgga tggtccggaa cagcgcccgc ttcatctcag cgcccttccc catcgtcttg
      301 gcctgttcat acatatcgaa ggcggtcggc aacttgccgg gaatcacggg ataccccacc
      361 atgaccgctt ccagcttgtt gccgaattcc ttttccagaa tcgccagccc ctcgccgtcg
      421 aaccggtggc aatgcgggca gtagaaatcc gcgaattcaa tcaattgcac tttccccggc
      481 ttgtgcgtcg acttctcgtc gctcagcacc tgaaagttgc ccttgagttc cgccttcccc
      541 gcctgcgcgg ttcccacgaa cccgagagcc gccaccgcca tcaggcccat caacagactg
      601 ccccgccatc cacgacgctt cgccatacgc tgcactcctt tttcaaactt ggttcggttc
      661 aacaggctca tcggcattat aaacaatgct cgtccactta tgcgtctgat ataatgccaa
      721 cgatgcgagt gaaacttcta caggggatcg gcattttgtg gttcgccgcg atcctggcgg
      781 cctgcgcgtc gtccggcgac atgcacgagt cggccgacgg cggcccgcac gttccgccgt
      841 tttctcaaat caaggccgca ccggactcgt tcaaagggca gaccctcgtg ctcggcgggc
      901 aagtgctcgc cgcgcgacgc atgaaagacg gcacgcggat cgaagtgctg cagttaccgc
      961 tcaacgactc gcaacaaccg acgctcgatc tgatgaaatc gcaggggcgc tttgtcgcgg
     1021 tccaacggga ctttctggat ccggcaacca ttccccaggg aacgttcctg accatcaccg
     1081 gtgagctcac cggctcgatg accttgccgc tcgacgagac cgactatacc tatcccgtca
     1141 tcgacatcaa atcgttccgc acctggattc catcgcaaga ctcgactatg tcccgtatcc
     1201 gtccctaccc ctattacagt ccgttctggc atccctactg gggaccctac gggcgctttc
     1261 cctactattg gtaaacccgg ccgcctgtcg atcatcgttt cgacaacggt gaagaaacga
     1321 caacgccccg tccatccctg cggatgaacg gggcgtgtgt actcgcacta cagacagggt
     1381 ctgttacttg atcatcagca gtttgccgcc gggctgcttg ggattgagct ggtcgcggta
     1441 ctcaagcgta ttgccctggg tttgattgaa caaatcgctg gtcggcacgc cgatgtactt
     1501 cgtctcaccg ggcttcaacc ggacctcggc ctttaagacg aacggcgacg ctgcattgac
     1561 tgtatcgatc atttgaaaac cacgttccgt cgcggtggta ttcgtgacct tgatcaacag
     1621 cggacgaccc ggccggactt tgaaatcgat gaccgtggtc ggcggatacc acgccttcat
     1681 gttgccgatc tcaacggcat ataattccgc atccacgacc aattccttaa acggctgacc
     1741 gacgacggaa ccggtttcga ggtcaccgac ttcgacaccg ccggcctgca gcgccaacgc
     1801 agacgttccg ctcgccaaca cgcccaacac gcccatgaaa aggaccgcaa tcctgctcaa
     1861 catagaacct cctgtcaggt taaagagatg aggacggacg atgctcctgt gccaggcacg
     1921 gaggaagacc ggtgccttcc aacacctggc caccgacaat gtgaccatcg acgatgagaa
     1981 acagcacttt catacgcttc gtattgtctg aaaattttcc gctggccacc gccagcactt
     2041 catgcgaccc gtcaggctgg ggctgatccg cttcgatctt tacgtagtac gaatcgaatc
     2101 cctcgaattc ccgattgaac gcctgcgccc gtaacaaccg ttccagtgaa tcgtcggcgg
     2161 cccacactcc tgacccgccg gccagcacga ggatctgaac cacggccacg aagagttttc
     2221 gcatggggga cccctcctag ggagtcgagt tatagcccca gcacgctcaa ccgtctacag
     2281 atgcgcgctt acggatgcct accgatgcta caggatcgtg tcgttgaaac gtggcttccg
     2341 cagccgacca gaacttggct gggcttgacc cgtctcacga agccgaacac gtcaactcac
     2401 gcaagcggaa ggacccccgg cacggttcca agggggaacg ccggatggga ctcacctccc
     2461 ccttggaaca gagatcgcgt tacttgccct ttgctttccc tttgtggctc gaagcctgtt
     2521 ccgcatgcgc cagggattcc tccgcatgtt tgacggcttc cttcgcatgc tccagcgctt
     2581 ctttcgcatg cggatcgttg ctggccttga ccgactcttc cagatgcttg atcgcttcct
     2641 tctcgtgctc cacgccttcc ttggcgtgct tgatcgtttc cttcacgtgc ccgccgccgt
     2701 cggccagggc gtatcctacc ggcgcagtcg ccaccagcgc ggacacggcg aacgcgagca
     2761 tgcctgaacg caatacagtc atcataattc ctcctgtgtg ataagtggat gtagcactcg
     2821 attgggcggc attctaccgc atttgtcatg tcgtgttcag taaggaaaga ggaggcccca
     2881 ccgaccgtgc gggcaacgca ggactgggaa aaagatggtg ggagagggaa gcgtatgggc
     2941 gtacgcttcc ctcatcggag ctcactgcgt ggtcagcgca gtccaaccgt cgagccccgt
     3001 ccaccgatca tcgaacgtca gcgggcgtca gccagggcag tcgacagacg ccgcatcggc
     3061 cacgatccgc catggcctgc tgagcgggcg aatcccagct cgcatacatc catgattcgt
     3121 actgcctcgt ccccgcgcca tccatcgtga gaccggcaca cttctccatc gcaaacacgg
     3181 acggcctggc ccgataggat ccggggcctt ccggataggg ctcggactcc acgacgaacg
     3241 tggtccccac gggaaacgat tcatagtccg gcgtgagcag tgccttgggg cacacgtaga
     3301 accggagccg tttccgcccg ccgggcgatt ccaccgtaga ggtcacgctc ggccagtccc
     3361 gatacccgaa cggaatcaag agcgtcgcag cgggaggcgc cggggaccag ccctcgctga
     3421 tatcgagggc gaccgccagc aatcgcacga ctgccacact caaccccgcc ctgtgttgag
     3481 cgccctgagc catctccgcc cgacgcggtg acgccaccgc agcactaacc ggcatggagc
     3541 acctcccttc ctgacgcctt cactccctat cgcgtgagtt ctccgaccgc aggcccctgg
     3601 cgcatggccg aaccctgccg cacgactccc ggctgaaccc gcgcccggat cgatgcggcc
     3661 cgcgcagcga acaatccggc ttcaaccggc tgcccgagag tatcgagcac gcgcgcatac
     3721 cgctccacca acaacccgac ggtgggatga tgcaagccca gttgcgcctg ttgaacgacg
     3781 agcgcgcgct gatacaacaa ctgcgcctgg gtccatagtc cctgctcatc ggcgagaaac
     3841 gccatattgc cgacaatcgt gccggtcaag gaggcctggg gtccggacgt ccgttccgca
     3901 atggccagtg cctcatcgaa atatcgttga gccgacgcgt agagtccttc ggcctggtag
     3961 aggcaggcgg cgttattcaa actcatcgcc accgccggat gttcatcgcc gaatcgcagg
     4021 cggcgcatct tcagcgcgcg caggtagaac ggttccgccc aggcatagag tccgcgcccc
     4081 ttgtacaaga cggcgaggtt gttgagcacg ggccccagat cggggtgatc cgtgccacgg
     4141 gcctcttcca tgagaatcag ggccaggtga tacatcgatt cggccgctgt ctcccgtccc
     4201 tgcttgcggt acaactcggc cagattgttc aggctgatcg cgacatcggg gtgcgccgcg
     4261 cccagcacct tgcgctcgat tgcaagcacc cgttcataca gcggctccgc gcggccccaa
     4321 tcgccttcgg catgggcgat ttccgccagg ttgtttaagg ccgccgcgac atcttcatgt
     4381 tccggtccca attgttgttc ccatatggcg agcgcacggg tgtacagcga ttgcgcctcc
     4441 tggagacggc cctggtcgtg atacagcaat ccgagattgt tggctgccgt ggcaacgcga
     4501 gggtcgtcgg ggctgacctg ctcagccccg cggagagcgc gctgcaagag ttgctcagcc
     4561 tcggcgtagt gcgccgcttc cctggccttc acaccgtcag tgaacaactg cttccaagca
     4621 tccggctcat tagcccagct cccatcaacg ccgcacaaag ccatgccgac caccaatgcc
     4681 atgagtaacc accgc
//
LOCUS       DAOQRM010000183         4650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_19501_length_4650_cov_12.387378, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000183 DAOQRM010000000
VERSION     DAOQRM010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19501_length_4650_cov_12.387378"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..594)
                     /locus_tag="PKK81_11675"
     CDS             complement(7..594)
                     /locus_tag="PKK81_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV33589.1"
                     /translation="MTVHRTARTLVFNKPYDVLPCFTDPEGRQTLGDYIDVSGVYAAG
                     RLDRDSEGLLLLTSDGALAHRITDPEHHLPKVYLVQVERIPDAAAVERLQQGVVVGGR
                     RTKPARVRLLLEAPQIPERPVPIRFRKHVPTAWLELTLREGMNRQVRRMTAAVGHPTL
                     RLVRVAIGPIFLGALLPGEWRELTKAETAEIYGYS"
     gene            complement(724..1341)
                     /locus_tag="PKK81_11680"
     CDS             complement(724..1341)
                     /locus_tag="PKK81_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33590.1"
                     /translation="MTTVTNPMHRQAPTRARFIVLALAGLLSLSGCQSPGTPLPPTLK
                     PSLAWPECPKIGGTYELQGEPLPGTMNFYRNRDNKLTLNAMLGVAVPADLAQEAVRVE
                     LVHEDTLELVNRFDGAITAHQLDLQPEDRVTCDRNQIRIHRTRSEGGVAEEPLRNISD
                     IAQELTLEGDGSLLVKTRIMNQSKSAFFSTPVPHEEYSARFRRLD"
     gene            1637..2503
                     /locus_tag="PKK81_11685"
     CDS             1637..2503
                     /locus_tag="PKK81_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNV33591.1"
                     /translation="MRWEGQRESRNIEDRRGMGAARSGVGLGLGGIVLVLAVSFLTGT
                     NPLTLLNLLNGVQEITAPSEPERPSPKGAPRDSLGKFASVVLADTEDTWRVLLPRLGS
                     TYEDPQMVLFTGAVRSACGTASSAVGPFYCPGDQKVYLDLSFFDEMAQRLGAPGDFAQ
                     AYVIAHEIGHHVQNLLGIAGKVTRLQQRGSTAEHNALSVRMELQADCFAGVWGYHAQH
                     NRNLIEPGDFEEGLRAAAAIGDDRLQKMGQGHVQPESWTHGSSEQRMTWLRRGLQSGD
                     PNNCDTFSGGRT"
     gene            complement(2612..3166)
                     /locus_tag="PKK81_11690"
     CDS             complement(2612..3166)
                     /locus_tag="PKK81_11690"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNV33592.1"
                     /translation="MLLGTRQYLILGLLAVAAGCATSVIPESLEPQVDKTVTFSQVLE
                     APDSYRGKVVVWAGEVLKAKAMKGGTQLEVLQVPLDDEQGPVTDRMESKGRFLALQKE
                     FLDPATMTDGTRVTIVGEITGASVEKIDEADYRFPTLDVKHLHRWDSRQVDDRRVIGP
                     WWNVFGGVGFGGGSRSGGGISIGF"
     gene            3323..4357
                     /locus_tag="PKK81_11695"
     CDS             3323..4357
                     /locus_tag="PKK81_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNV33593.1"
                     /translation="MSRSSLDYVKQIVRGSLGMMAAVSLATGLFTNEAAAIEATMLER
                     AKLATIGVLEDTQDQRTPDKPGKILVRGTGFHLRDGYIVTARHAAEKQDVTTGTVIQK
                     QIHVLTTDLHEIPADLVGDSAFMDVVVYRVAEAHRAKLRATVPFASGDVQPGQEVFTV
                     GYPMGWGPTMSFGHLGNTNTFLQTVDTRLIQADVAACSGNSGGGLFNDKGEVVGIMHA
                     IIQTERDDSTAHCSRMAFAIPAMLAERIVTAALEGKPMAFSKMGIHMVAVKDGTRWRM
                     AVKDVMEPAKSAGIEKHDIIIAVDDTDIQDAAHLKNYLIERTKPGQQVSVKVRRIDTN
                     LTFTVTLAGG"
     gene            complement(4367..>4650)
                     /locus_tag="PKK81_11700"
     CDS             complement(4367..>4650)
                     /locus_tag="PKK81_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HNV33594.1"
                     /translation="DEPTSGLDLKSCFQYLDLVRTQIHKGKTVVLVTHHLHEIPPEID
                     RVIFLKGGKILADGPKLNLLTDDRISTLFDRPIVLAQANGWYQALPGLS"
BASE COUNT          981 a         1463 c         1318 g          888 t
ORIGIN      
        1 cctctctcag gaataaccat aaatctcagc tgtctctgct ttcgtaagtt ctcgccactc
       61 accgggcagg agcgccccca agaaaatcgg gccgatcgca acccgcacca gtcgcaatgt
      121 cggatggcct accgctgccg tcatccgtcg aacctggcga ttcatgcctt cgcgcagggt
      181 gagctccaac catgcggtgg gcacatgctt tcggaatcga atgggcaccg gccgctccgg
      241 gatctgaggc gcttccagca acagacgaac ccgagcaggc ttcgtgcgtc tcccgccgac
      301 caccacccct tgttgcagcc gttccacggc agccgcatcg gggatacgct caacctgtac
      361 aagatagact ttgggaagat ggtgctcggg atcggtaatg cgatgggcta acgccccgtc
      421 cgaagtcagc agcagcaggc cttcgctatc ccgatccaat cgaccggccg catagacgcc
      481 agacacatcg atgtagtcgc ccagggtctg acgcccctcc ggatcggtaa agcaaggcag
      541 cacatcgtag ggcttgttga acaccagcgt gcgggcagtg cggtgaaccg tcatcagaac
      601 gggactctcc gtatcgccgc aatccttcgc ctcatcacag aagtggacgg acagcaccgc
      661 aacatgatga aggagtgcgt gtgacgaatg accagtaacg agggacctat cgcgggaccg
      721 cgactaatcg agtcgccgga agcgcgcgga gtattcctca tgaggaaccg gtgtgctgaa
      781 gaaggcggat ttactctgat tcatgatgcg ggtcttgacg agcagacttc cgtccccttc
      841 cagagtcaat tcctgtgcga tgtcggaaat gttgcggagg ggctcttcgg ctaccccgcc
      901 ttccgagcgg gtccggtgaa ttcggatctg attgcgatcg caggtgaccc gatcctccgg
      961 ctgtagatcc aattgatggg ccgtgatggc gccatcaaac cggttgacca gttccagggt
     1021 gtcctcatgg accaattcca cgcgcacggc ctcctgcgcg agatccgcag gcaccgccac
     1081 gcccagcatg gcattcaagg tgagcttgtt gtcccggttc cggtaaaaat tcatcgtgcc
     1141 ggggagtggc tctccctgaa gctcatacgt tccgccgatc ttgggacatt ccggccacgc
     1201 caggctcggc ttcaaggtcg gagggagcgg cgttcccggt gactgacagc cggagaggct
     1261 aagcagcccc gccaacgcca gaacaatgaa acgcgcccga gttggggctt gtcgatgcat
     1321 cgggttggtc acagtggtca tggaatgagt ctagcgaagc gaccgttggc ctgtctagtc
     1381 cgattatgca ggctcttctg gacaaccaat ccggtgcgtc aacgtgagac aatcggctac
     1441 cgcccagcac ggaggcgcag gctgaggccc tgcactcacg cccactgcca gcaccacaat
     1501 cgccgaacgg cttatgagta ttgcgcgtta gggacacccc aggcttgctc aagtatgaca
     1561 aatgtggttc catcagcgtg ccctcatagt cctcgacgag acgttacgag cctgatattc
     1621 accaaggagt cctgccatgc gatgggaagg tcaacgcgag agccggaaca ttgaagatcg
     1681 tcgagggatg ggtgcggcca ggtccggcgt aggccttgga ttagggggca tcgtcctggt
     1741 cctcgccgtg agcttcctga ccggaaccaa tcccctgacg ctgctcaact tgctcaacgg
     1801 cgtccaggaa atcaccgcgc catccgaacc ggagcgcccg agcccgaaag gtgcaccgcg
     1861 tgacagcctc ggcaaatttg cctccgtggt tctggccgac accgaagaca cctggcgagt
     1921 tctgctccct cgattgggca gtacgtatga agatcctcag atggtcctct tcaccggcgc
     1981 agtacgatcc gcctgcggca ccgcctcgtc cgccgtgggc ccgttctact gtcccggcga
     2041 tcagaaggtc tatctggatc tttccttctt tgacgaaatg gcccagcgac tcggcgcgcc
     2101 cggcgacttt gcccaggcct atgtcatcgc gcacgagatc gggcaccatg tccaaaacct
     2161 gctcggcatt gcggggaaag tcacccgact tcaacaacgt ggctcgacgg cagagcataa
     2221 tgcgctctcc gtgcgcatgg aattgcaggc cgattgtttt gctggcgtat ggggctacca
     2281 tgcccagcac aaccgaaacc tgatcgaacc aggcgatttc gaggaagggc tgcgagctgc
     2341 cgccgcaatc ggtgatgacc ggcttcagaa gatggggcaa ggccacgtac agccggaaag
     2401 ctggacccac gggtcatccg aacaacgcat gacctggttg cggcgcggtc tccagtccgg
     2461 cgatcccaac aactgtgata ccttctccgg cgggcgcacc tagttccgcc acgcttcgtg
     2521 agcaaaacga gttcctgacc ggctatctcc gctcagccgg cgcagccact ggcgacatat
     2581 ttatggggaa tccccaaaaa ccggctggtc atcaaaaccc gatactgatc ccgccgcccg
     2641 accggcttcc gcccccgaaa ccgacgccgc cgaacacgtt ccaccaaggt ccgatcaccc
     2701 ggcggtcatc cacttgcctg gaatcccatc gatgcagatg cttcacatcc agcgtcggaa
     2761 aacgatagtc cgcctcatct attttttcga ccgacgcgcc cgtaatttcg ccgacaatcg
     2821 tcaccctggt gccgtccgtc atcgtggccg gatcgaggaa ctctttctga agcgccagga
     2881 accgcccttt cgactccatg cggtcggtta ccggcccctg ctcgtcatcg agcgggacct
     2941 gcagcacctc cagttgagta ccacccttca tggctttcgc cttgagcact tcaccggccc
     3001 acactacgac cttcccgcga taagaatcgg gggcttccaa gacctgactg aacgtcacgg
     3061 tcttatcgac ttgcggctca agcgattcgg gaatgaccga cgtcgcgcaa ccggcggcca
     3121 cagcgagcag gcctagtatc agatactgtc ttgtgcctag cagcatgagc tcagtatagc
     3181 gcccgtcgca agaggatgcc acggcggaat ctcccctcga cctgacgttc gccatcactg
     3241 acgcacgacc acatttcatc cgctccggcc tctaggctat actgcagctc tgacgacatc
     3301 ctcacgaaag gaacggttcc ctatgtcgcg ttcgtcactc gattacgtca aacagattgt
     3361 gcggggttcg ctcggtatga tggccgctgt gagtctggcc acaggcctct tcacgaacga
     3421 ggcggcagcc atcgaggcca cgatgctgga gcgcgccaaa ctggccacca tcggtgtcct
     3481 ggaggacacg caagaccaac gcacaccgga taaaccgggc aagattctgg tgcgtggaac
     3541 cggctttcat ctccgcgacg gctacattgt caccgcccgc cacgcagcag aaaagcaaga
     3601 tgtcacgacc ggcaccgtca tccaaaaaca gatccacgtc ctgaccaccg acctccatga
     3661 aatccccgcc gatcttgtcg gcgacagcgc gttcatggac gtcgtggtct atcgcgtcgc
     3721 tgaagcccat cgcgccaagt tgcgcgcaac ggtccccttt gcctccggcg acgtgcaacc
     3781 ggggcaagaa gtcttcaccg tgggctaccc catgggctgg ggcccgacta tgtcgttcgg
     3841 acacctgggc aatacgaaca ccttcctcca gaccgtggac accagattga ttcaagccga
     3901 cgtggccgcg tgcagcggca actccggtgg cggcctcttc aacgacaagg gtgaagtggt
     3961 cggcatcatg cacgccatca tccagaccga acgggacgac tcgaccgcgc attgcagccg
     4021 catggccttt gccattcccg cgatgctggc tgaacggatc gtcaccgccg ctctggaagg
     4081 caagcccatg gccttctcga agatgggcat tcacatggtc gcagtgaaag acggcaccag
     4141 gtggcgcatg gcggtgaaag acgtgatgga accggccaag tccgcgggca ttgaaaaaca
     4201 cgacatcatc attgccgtcg atgacaccga tattcaggat gccgcacatt taaagaacta
     4261 tttgatcgaa cgaaccaagc ccgggcaaca agtcagcgtc aaggtccgcc gcatcgatac
     4321 caatctgacc tttacggtaa cgctggcagg tggatgatcc aacctgttac gacaggccag
     4381 gcagggcttg ataccagccg ttggcttggg cgagcacgat gggacggtca aagagggtgc
     4441 tgatgcggtc gtcggtcagc aggtttagtt tcgggccgtc ggccagtatt ttgcccccct
     4501 tcagaaagat cacgcgatcg atctccggtg ggatttcgtg cagatggtgg gtcacaagca
     4561 caacggtctt gcctttatga atctgtgtgc gcacgaggtc caagtactgg aagcaggatt
     4621 tgagatcgag cccgctggtc ggttcatcca
//
LOCUS       DAOQRM010000184         4626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_19663_length_4626_cov_18.206957, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000184 DAOQRM010000000
VERSION     DAOQRM010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19663_length_4626_cov_18.206957"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PKK81_11705"
     CDS             <1..1008
                     /locus_tag="PKK81_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV33595.1"
                     /translation="GLDPREEKDPDAVRRAIHPEDRERVIEEFAQAVAEGQPYETVFR
                     FLHPDGTIRKVLSRRIPIRDHSGEVVMYQGFNVDITALDLMQSRLRRAERLATLGQVA
                     AGIAHEIRNPLVGIGSTTSLLLDDTDPTDARRPDLAVILQETKRLDRIVNQIIDYARP
                     REIVAFAFDMAQLVQEVMKVLDEPLTRKQVTIRLASPDSPYTIQADRDQLKQVLLNVL
                     QNAIEASPSGKTITVTLVQQARGVEPGLEVTVADRGTGISPTHLPHVFEPFFTSGKPR
                     GTGLGLAICRNILEAHGGDIALDSEVGRGTTVRVWAPLRQQPPRMQEEEQHAGHDLRH
                     G"
     gene            980..2371
                     /locus_tag="PKK81_11710"
     CDS             980..2371
                     /locus_tag="PKK81_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV33596.1"
                     /translation="MQATIYVTDDEPAIRSAIVKRLSRRHHTVTGFESGEDVIRAVTQ
                     HAPDLILLDLKMPGMGGVETLRQLRPIAPQTLIIMLTAYGTVEDAVEAMRLGAYDFLI
                     KSVDLSGVDPVVDRAIEFLTLRHRVEFSLEDQNSAYALSNIDARSPAMQLLLGQVRDV
                     AENAKSTVLLQGETGTGKEFLARVIHCNSPRASGPFVAVNCTAIPRELFESELFGHER
                     GAFTGAHQRKRGLLEKAEGGTLFLDEIGDLDPAMQAKLLRVLQERTFRRVGGTEDLSV
                     DFRLMTATNRDLKKDTARGSFREDLFFRLNVVTFELPPLRVRTEDILPLSMQAMLRFG
                     KEFGKEVVDIEPEAQELLQRYSYPGNIRELQNIIERAMILCHDKTLTAGCLPRELREQ
                     APHIAVAMAQGEHPSLRIEMVLGQQTLADIESALIEEVVRLSDHNKTLAAKHLGLTRF
                     ALDRRLKKQMEQD"
     gene            2454..>2672
                     /locus_tag="PKK81_11715"
     CDS             2454..>2672
                     /locus_tag="PKK81_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33597.1"
                     /translation="MTGKHNSWIVPGIVGLTCGLFALDLFMPLGVAIGALYAGIVLLA
                     SFSPNPRLPLLTAACATGLLIVGAVAGPR"
     assembly_gap    2673..2772
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2773..3429
                     /locus_tag="PKK81_11720"
     CDS             <2773..3429
                     /locus_tag="PKK81_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNV33598.1"
                     /translation="LRDLAARLLTVQEEERRRISRDLHDDINQRLAMLVVQAESLESS
                     LPPSAGDCSKELRSIQDRLTELSDDVRHLAYQFHPSILDDLGLTVALQRLVDECATRA
                     NLQGTFEVSPAPPTVPQTVSTCLYRIAQESLANVMKHARANKVIVSLASTADAITLTV
                     QDDGVGFDTQQLANSHRGLGLISMAERLRLVRGTVTIDSIPHQGTRLYIKVPHAEVPV
                     "
     gene            3426..4070
                     /locus_tag="PKK81_11725"
     CDS             3426..4070
                     /locus_tag="PKK81_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV33599.1"
                     /translation="MTTPPRVLLADDHTLVLEGFRRIVEQRCEVVGAVEDGRALLDAA
                     TRLRPDLILLDISMPLLNGVDAARQLKKLLPDVKLIFVTMHADPAYVSEAFKAGASAY
                     LLKRSAARELEQAIDSVMKGQYFVTSLLTKDLVTTLSAGQAGLFAQRQELTPRQREVL
                     QLIAEGRTIKEIASLLNISPKTVEFHKAQIIFHLNLRTTAELTKYALAHGLTSA"
BASE COUNT          908 a         1493 c         1312 g          813 t
ORIGIN      
        1 ggactcgacc cgcgcgaaga gaaggacccc gatgcggtcc ggcgcgcgat ccacccggag
       61 gaccgggagc gggtcatcga ggaattcgcg caggcggtgg cggagggaca gccctatgaa
      121 acggtcttcc ggtttctcca tccggacggc accatcagga aagtcttgag ccggcgcatc
      181 ccgattcgcg atcattccgg agaggtcgtc atgtaccagg ggttcaacgt ggacatcacc
      241 gcgctcgacc tcatgcaatc gcgactgcgg cgggccgagc gactggccac actcgggcag
      301 gtggccgcag gcatcgcgca cgaaatcaga aacccgctcg tcggcatcgg ctcgaccacg
      361 tccctcctgc tcgacgacac cgatccaacc gatgcgcgcc gccccgatct ggccgtcatt
      421 ctgcaggaga ccaaacgcct cgatcggatc gtgaaccaga tcatcgacta tgcccgcccc
      481 cgtgagatcg tggcgttcgc tttcgacatg gcacagctgg tgcaagaggt catgaaggtc
      541 ttggatgagc cgctcacgcg caaacaggtg acgatccgtc tcgcgtcgcc ggactccccc
      601 tacaccattc aggccgatcg cgatcagctg aaacaagtgc tgctcaacgt cctgcagaac
      661 gcgatcgaag ccagcccctc cggcaagacg attaccgtca ccctggttca gcaggcccga
      721 ggggtggaac cgggactgga agtcaccgtc gcagaccggg gaaccgggat cagccccacc
      781 cacctcccgc atgtattcga accgtttttc accagcggca aaccgcgggg gacaggatta
      841 ggacttgcca tctgccgtaa cattctcgag gcacacggcg gagacattgc cctggacagt
      901 gaagtgggac gcggcaccac cgtgcgcgtc tgggccccct tacgacaaca gccaccacga
      961 atgcaggaag aggaacagca tgcaggccac gatttacgtc acggatgacg aaccagccat
     1021 tcgatccgcc atcgtcaaac gcctttcgcg acgccaccat accgtgaccg gcttcgaatc
     1081 gggcgaggat gtgatccggg ccgtcaccca acatgcgccg gacctcatcc tgctggactt
     1141 gaagatgccc ggcatgggag gcgtcgaaac gctccggcag ctccgcccga tcgcgcccca
     1201 gacgctgatc atcatgctga cggcctatgg cacagtggaa gatgcggtcg aagcgatgcg
     1261 gctgggcgcc tacgatttcc tgatcaagtc ggtcgacctc tccggtgtcg accctgtcgt
     1321 cgatcgcgcg atcgaattcc tcaccctgcg ccaccgggtc gaattttcgc ttgaagacca
     1381 aaacagcgcg tacgcgctct ccaatatcga tgcgcgcagt ccggcgatgc agctcctgct
     1441 cggccaggtt cgggatgtgg cggagaacgc caaatccacc gtgctgctcc aaggcgaaac
     1501 gggcaccggc aaggagttcc tcgcgcgggt catccattgc aatagcccgc gcgcgtccgg
     1561 ccccttcgtc gcggtgaatt gcacggccat tccgagggag ctttttgaaa gcgagctgtt
     1621 cggacacgag cggggcgcct tcaccggcgc ccatcagcgc aaacgcgggt tgctggagaa
     1681 ggccgaaggc gggacgttgt ttctggacga aatcggcgac ctggacccgg ccatgcaagc
     1741 gaagctgctc cgcgtgctgc aggaacgcac cttcaggcgc gtgggcggca cggaagacct
     1801 ctccgtagac ttccgcttga tgaccgccac caatcgcgac ctcaagaagg acacggcgcg
     1861 cggcagcttc cgggaagatc tcttctttcg cctgaacgtg gtgaccttcg aactcccgcc
     1921 cctgcgcgtc cgcacggagg atattctccc gctttccatg caggccatgc tgcgattcgg
     1981 caaagagttc ggcaaggagg tcgtcgacat cgaacccgag gcgcaagagc tccttcagcg
     2041 atacagttat ccgggcaata ttcgcgaact ccagaacatc atcgaacgcg ccatgattct
     2101 ctgccacgac aaaaccttga ccgccggctg cctcccgcgc gaattacgcg agcaggcccc
     2161 gcacatcgcc gtggccatgg cgcaaggcga gcacccctcc cttcgcattg agatggtgct
     2221 gggacaacag acgctggccg atatcgagtc ggccctgatc gaggaggtcg tgcgcctctc
     2281 ggatcacaac aaaaccctgg ccgccaaaca tcttggattg acgcggttcg ccctggaccg
     2341 gcgcctcaag aagcaaatgg aacaggactg agcatgcagg cggcagggag tgcattccgg
     2401 ggttgaaccg cgtcactcgg gaacggataa tggacacatg aatcaggcag ccgatgaccg
     2461 gcaagcataa cagctggatt gtcccaggca tcgtcggatt gacctgcggc ctctttgcgc
     2521 tcgatctgtt catgccgctc ggggtcgcca tcggcgcgct ctatgccgga atcgtgctac
     2581 tggcctcatt ctccccgaat ccaagacttc cgctgctcac cgccgcctgc gccaccgggc
     2641 tgctcatcgt cggggccgtg gcggggcctc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnttgcggga cctggcggcc cgcctcctca cggtccagga ggaggaacgc
     2821 cggcgcatct cccgggatct ccatgacgac atcaatcagc ggctggccat gctggtcgtc
     2881 caagccgagt cgctggagag cagcctcccc ccctcggcgg gcgactgcag caaagagctg
     2941 cgctcgatcc aggaccggct caccgagctc tcggacgatg tgcgccatct ggcctatcag
     3001 tttcatccgt ccattctgga cgacctggga ctgaccgtcg ccttgcagcg cctggtcgat
     3061 gagtgtgcga cacgcgcgaa ccttcaaggc acgttcgaag tgtcgccggc gccgccgacc
     3121 gtgccgcaaa ccgtttccac ctgtctctac cgcattgccc aggagagtct ggcgaatgtg
     3181 atgaaacatg cgagggcgaa taaggtgatc gtttccctcg cctcgacggc cgatgccatc
     3241 acgctcaccg tgcaggatga cggagtcggt tttgacacgc agcaactggc gaacagccat
     3301 cgcggtctcg gcctgatcag catggcagag cgcctccgtc tggtccgcgg caccgtcacc
     3361 atcgattcga tccctcacca gggcacacgc ctctacatca aagtaccgca cgcggaggtt
     3421 cccgtatgac cacgccccct cgagtgttgc tggccgacga ccatacactg gtactggaag
     3481 gattccggcg aatcgtggaa caacgttgtg aggtcgtggg ggcggtcgaa gacggccgcg
     3541 cgctcctcga cgcggccacg cggctgcgcc ccgacttgat tctgctggat atctccatgc
     3601 cgttattaaa tggagtcgat gccgcgcgcc aattaaagaa gctgctgccc gacgtgaaac
     3661 tcatcttcgt caccatgcat gcggacccgg cctatgtgag cgaggccttc aaggccgggg
     3721 catcagccta cctgctcaaa cgatccgcgg ccagagaact ggagcaagcc atcgactccg
     3781 tcatgaaggg acagtatttc gtcacctccc tgctgaccaa ggatctggtc acgaccctct
     3841 ccgcagggca ggccggcctg tttgcacaac ggcaggaact gacgccgcgc cagcgggaag
     3901 tgctgcagct cattgccgag ggacgcacca tcaaggaaat cgcctcgctg ctgaatatct
     3961 cgccgaaaac cgtcgagttc cacaaagccc agatcatctt tcatctcaac ctccgcacca
     4021 ccgcagaact gacgaagtac gcgcttgcgc atgggctgac atcggcctaa ccaggactat
     4081 ttatgaatac ctgctccgtc ggccgatcct cccgccaggc ggggccgttc tcgttcgacc
     4141 tcctcgacgg actgcacgta cgcctgcgtc ggtctccagc gcctcgcgac ggcattcatg
     4201 aataatccgg gctcgtcgct caactgaggt cagcctcgat tccagccggc tcggattcat
     4261 cgctgaaatc ggcctcggga catttcacct ccgacctagg agatcgccta gttgtctctc
     4321 ctcttcacgt ttgttaccct ccatgcatat gtttcgccgt tccccatgcc tgtccgtcat
     4381 gctggctcgg tacggtcgaa ggaggtctgg gcgatggact ccgcagccac acaatgccag
     4441 gtttgctggc gagtgcggga taacaggacc gaaggaagct cctgtgcggt atggtcagac
     4501 ttgcagacgt tcatggacaa ccacgatgtg acatcccatg gccccagctt ttccgaaggg
     4561 tattgccccc agtgcaccac cctgtatcat cgactcacgc aagaacatct ctacgacgca
     4621 taacag
//
LOCUS       DAOQRM010000185         4616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_19742_length_4616_cov_16.192721, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000185 DAOQRM010000000
VERSION     DAOQRM010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19742_length_4616_cov_16.192721"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1586)
                     /locus_tag="PKK81_11730"
     CDS             complement(240..1586)
                     /locus_tag="PKK81_11730"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNV33600.1"
                     /translation="MTSSITPSWPKLTGRQEFEFLAELVMSHSTGDHTYLSLRDVHGG
                     TTRFANNQVIQNVNQRRGALSITVAFGRQHGTASTTDFTAGAVRETLKQAECIARISP
                     EDPEYLPPVGPQTYLSLPTSRPETSAAGPARRLDYAREAIGQCKMENLNAAGTVSSST
                     AIVGLAADTGLRAYEERTEARFSLTVQAGEATGWSAAAHRSIDRLHVQERTLAAIIKA
                     KRGADEPRELPPGRYTVILEPAAVAGLLSWMIWMLDAKSFYKGTSPFSRKLNTRILDR
                     RLSLFNQPAHADLLGHGFTSEGLPVIESSWIEAGVLNQLLHDRFTAQEHGIDPLTTLE
                     SPYLSGERPVGTRVDDLIRTTQRGILVTNFWYIRPVNPSDLTLTGMTRDGTFLIENGE
                     ITSAVRNFRFHESPLRAFNQVETYTAPVEAVTSETGKFLVPAMRINGFNFSSVTRF"
     gene            complement(1583..3046)
                     /locus_tag="PKK81_11735"
     CDS             complement(1583..3046)
                     /locus_tag="PKK81_11735"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNV33601.1"
                     /translation="MSGPLWDELAELALSRVRAAGVEYADIRLLDTTTRTISGEDRRI
                     AHIRESTDRGFGIRVLHRGAWGFAASSVISLEEIPRVADLAVEIAKGSASLAITKVHL
                     APEPVHRDRIVTPCRLDPFAVPLEEQTSLLLSTMDVIQRHQGVVRSHASFWAQRDRKL
                     FVSTEGSHLEFSLLAVQGECSATAVQDGRFATRSFNSPHLRMGYELIRDTDFASEGAR
                     IAEQAVEKVRAPVIEAGRYDLVLDPEHLSLTMHESCGHPSELDRALGYEANYAGTSFL
                     TPDKRGTYRYGSSHVNLVADNTEPHTLAATGYDDDGVSCQKWDIVREGLFVGYCTNRE
                     VAPRINEERSRGSNRADSWGSVPMVRIANIGLEPGTATLDELLADVKRGIYIEGHGSY
                     SIDQRRYNFQFGGDAFWLIENGRRTHMVRDVIYHGITPEFWGRCDGVADRSHRRRYGF
                     ITCGKGQPGQSGWMTHAASHARFRRVDVIAGEAKANE"
     gene            3162..3473
                     /locus_tag="PKK81_11740"
     CDS             3162..3473
                     /locus_tag="PKK81_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33602.1"
                     /translation="MFSPEQTPRRRIIELLTGTLLSSHQLAQLLGMPERQVEDHLTHI
                     VKTLSRDPARTFLLEPSECQDCTYVFRDRTRLTRPSRCPRCRSEAITSPRFGIREEDR
                     A"
     gene            3590..3814
                     /locus_tag="PKK81_11745"
     CDS             3590..3814
                     /locus_tag="PKK81_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33603.1"
                     /translation="MNRVAAWIIPSLLLVLPACGPDQAALLLETAQFEERQTNRAHAK
                     ELYEEIVRQYPDSPAAKTARARLTELTDKP"
     gene            complement(4144..>4616)
                     /locus_tag="PKK81_11750"
     CDS             complement(4144..>4616)
                     /locus_tag="PKK81_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNV33604.1"
                     /translation="FWYFQLQAAAIAFFSLPPLVVMQNPHPPFHFWDLIGFLWWAAAV
                     MGEAVADWQLAAFRSKPWNTGRVCRIGLWRYSRHPNYFFEWLHWWSYVWMALAIPTGG
                     WGLTLIGPMVMGVALLKVTGIPWTEAQSMTSRGEEYAEYQRTTNAFFPWFPRRS"
BASE COUNT          962 a         1474 c         1318 g          862 t
ORIGIN      
        1 cgcgcgcaac ggcgaaggtg caaacggatt cacgccgccc ttctcggact tgtccggagc
       61 gaaggtgagg agatgaatcg gtttttcggc caaacgctgc tgcttacgct tcatagggac
      121 accggcatct tatcggtcgg tactcgatca ttcattactc tttccaatag tccgagtcaa
      181 tggccggaac aggttttttg ccgtccggag cccccggatt cctgtgacct cgaccaggct
      241 tagaatcgag tgacgcttga aaaattaaag ccgttgatcc gcatggcagg caccaggaac
      301 ttcccggttt ccgacgtgac cgcttcgacc ggggcggtgt aggtttccac ctggttgaag
      361 gcgcgcaggg ggctctcgtg aaaccggaag ttccggacgg ccgacgtgat ctccccgttt
      421 tcaatgagga aggtgccatc ccgcgtcatg ccggtgagtg tcaggtcgga cggattgacg
      481 gggcggatgt accagaaatt cgtcaccagg atgccccgct gggtcgtgcg gatgaggtcg
      541 tccacccttg tgccgacagg acgttcaccg gataagtagg gagactccag cgtcgtcagt
      601 ggatcgatcc catgttcctg cgcggtaaac cggtcgtgca acaactggtt cagcaccccc
      661 gcctcaatcc aactggattc gatgaccggg aggccctcac tcgtaaatcc gtggccgaga
      721 aggtcggcat gggccggctg attgaacagg gacagtcgac ggtcgaggat ccgggtgttc
      781 agttttctgc tgaacggact cgtccccttg tagaaggatt tcgcatccaa catccagatc
      841 atccagctca gcagcccggc gacagccgcc ggctccagga tgaccgtgta ccggccgggc
      901 ggcaattccc gcggttcgtc cgcccctcgt ttggctttga tgatggccgc cagcgtgcgc
      961 tcctgcacat gcaaacgatc gatggatcga tgggccgccg ccgaccagcc ggtggcctcg
     1021 ccggcctgca ccgtcagact gaaccgcgcc tcggtgcgtt cctcgtaggc ccgcaatccc
     1081 gtgtcagccg ccaaccccac gatggccgtg ctggacgaca ccgttccggc cgcgttcaga
     1141 ttctccatct tgcattggcc gatggcctca cgcgcatagt caagccgccg tgccggtccg
     1201 gctgcgctcg tttcgggacg cgaggtcggc agcgatagat aggtctgtgg cccgacggga
     1261 ggaagatatt cgggatcttc gggagagatg cgggcaatgc actcggcctg cttgagggtt
     1321 tcccgcaccg cgccggcggt aaaatccgtc gtgctggccg tgccatgttg tcgcccgaag
     1381 gccaccgtaa tcgacagggc gccgcgccgc tgattcacat tctgaatgac ctgattgttc
     1441 gcgaagcgag tcgtgccgcc atggacatcg cgcaacgaca gataggtgtg gtcacccgtc
     1501 gagtgactca tcaccagctc agccaggaac tcaaattcct gccggcccgt caacttcggc
     1561 catgatggag tgatcgacga cgtcattcgt tggccttcgc ttctccggca atgacatcca
     1621 cccgcctgaa ccgggcatgg gaggcggcat gggtcatcca gcccgattgg cccggttggc
     1681 ctttgccgca ggtaatgaat ccataccggc gacgatggct gcgatcggcg acgccgtcgc
     1741 atcggcccca aaactcaggg gtaatgccat gatagatcac atcccgcacc atgtgcgtgc
     1801 ggcgaccgtt ctcaatcagc cagaacgcat cgcccccgaa ctgaaaattg tatcgtcgct
     1861 gatcgatact ataggagccg tgaccttcga tgtagatgcc ccgcttcaca tcggccaaga
     1921 gttcatcgag cgtcgccgtg cccggctcca gcccgatgtt cgcaatacgc accatcggca
     1981 cgctccccca actatcggcc cggttcgatc cgcgtgaacg ctcctcgttg atacgcggcg
     2041 ccacctcgcg gttcgtgcag tacccgacga agagtccctc ccgcacgatg tcccacttct
     2101 ggcaactcac tccatcgtcg tcgtaccccg tcgccgccaa cgtgtgcggt tcagtgttgt
     2161 cggccaccag gttcacgtga gacgaaccat accggtaagt gccgcgttta tccggtgtca
     2221 gaaaactcgt accggcgtaa ttcgcctcgt agccgagcgc gcgatccaat tcactgggat
     2281 gtccacagga ctcatgcatc gtcaacgaca gatgctccgg atcgagcacc agatcgtatc
     2341 gcccggcctc gatcaccggc gcccgcacct tctccacagc ctgctcggcg atgcgcgcgc
     2401 cttcgctcgc aaaatcggtg tcgcggatca actcataccc catgcgtaaa tgcgggctgt
     2461 tgaacgaacg cgtggcaaaa cgtccatcct gaaccgctgt ggcactgcac tcaccctgca
     2521 ccgccagcaa gctgaactca agatgggagc cttcggtaga gacaaagagt ttacggtcac
     2581 gttgggccca gaaactggcg tggcttctga cgacgccctg gtgtcgctga atgacatcca
     2641 tcgtgctcag cagcaacgac gtttgttctt ccagaggaac cgcgaaggga tccagtcgac
     2701 agggagtgac gatacgatcg cgatgcacgg gctcaggcgc cagatgtacc ttcgtgatgg
     2761 cgagcgaggc cgatcctttg gcaatttcta cggcgaggtc cgccacacgt ggaatttctt
     2821 cgagggaaat gacggagctg gccgcaaatc cccaggcgcc tcgatgcagc acccgaatgc
     2881 cgaacccacg atcggtgctc tcgcgtatgt gggcaatgcg ccggtcttca ccgctgatcg
     2941 tccgcgtcgt cgtatcgagc agacggatat ccgcatactc gaccccggcc gcgcgcacac
     3001 gagacagcgc caattcggcc aactcgtccc acagcggtcc actcatagca gcatctctcg
     3061 ttgcggaagg aagcccggaa agtcttgcgt agtataacaa gatcgcgggg gccgattcct
     3121 gccttttcat cgacaggccg ttcacctatg atgctcgccc tatgttctcc cccgaacaga
     3181 caccgcgacg gcgaatcatc gagctgctga ccggcacatt gttatcttcc catcaattgg
     3241 ctcagctcct cggcatgccg gaacgccagg tggaggacca tttgacgcac atcgtgaaaa
     3301 ccctgtctcg cgatcccgcg cggacattcc tgcttgaacc gtcggaatgc caggattgca
     3361 cgtacgtctt ccgcgatcga accagactca cccgtcccag ccgctgcccg cgctgccgca
     3421 gcgaggccat tacctccccg cgattcggca tccgcgagga agaccgcgcg taatatgaca
     3481 tgacggaaca ggactgcctt cttgcaaccg ccgatgtctt gagcgagact acgcagaggc
     3541 ctcatctcgt gacgacattg cgcgacatgt cagccgaaag gaccgccgca tgaacagagt
     3601 cgctgcctgg atcatcccga gtctcctcct ggtcctgccc gcctgcggtc ccgaccaggc
     3661 tgcgctgctg ctggagacgg cccagttcga agaacgccag accaatcgag cgcacgcaaa
     3721 agaactctac gaagaaatcg tccgtcagta cccagacagc cccgccgcca aaacggcgcg
     3781 tgcacgactg actgaactca ccgacaaacc gtagcggcag ccatttatcc ccttgtgtag
     3841 ccgcaggatg cgcaaaaagt tcgtccagca aggccgcagc gatcaaagag gcgaatcgta
     3901 ctcttgtggt acggcgagcc tcgacgagaa gcgagaacgc cgctggcgta atttctcggc
     3961 atcctgttaa cccggattat tcatgcatgc cgtcgcgagg cgttcgagac ggaagcccgc
     4021 cgaggcggct cgcacgtaag gccgacgcag gcgtactggc agtccgtcga ggaggccgaa
     4081 cgagaacagc ctcgccgggc gaagggatcg gacaccggag taggtattca tgaatagtac
     4141 gggttaagac cgtcgcggaa accagggaaa gaacgcgttg gtcgttcgct gatactccgc
     4201 atattcctca ccacggctcg tcatgctctg cgcttccgtc cagggaatgc cggtgacctt
     4261 cagcagcgct acgcccatga ccatgggacc gatcagcgtc aggccccagc ctcccgtagg
     4321 aatcgccagc gccatccaca cataactcca ccagtgcagc cattcgaaaa aatagttggg
     4381 atgccgtgaa tagcgccaca gaccgatccg gcacacacga ccggtattcc acggcttgct
     4441 gcgaaacgca gccagctgcc aatcggccac ggcctcaccc atgaccgccg cagcccacca
     4501 gagaaatcca atcaggtccc aaaaatgaaa cggcggatgg gggttctgca tgaccacaag
     4561 cggcgggaga gaaaaaaacg cgatggcggc agcctggagc tggaaatacc agaaca
//
LOCUS       DAOQRM010000186         4556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_20123_length_4556_cov_18.187514, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000186 DAOQRM010000000
VERSION     DAOQRM010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20123_length_4556_cov_18.187514"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /gene="pyrG"
                     /locus_tag="PKK81_11755"
     CDS             complement(<1..167)
                     /gene="pyrG"
                     /locus_tag="PKK81_11755"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF018158.2"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HNV33605.1"
                     /translation="MSKLIFVTGGVVSSLGKGLASASIGNLLESRGLKITFLKLDPYI
                     NVDPGTMNPYQ"
     gene            complement(185..955)
                     /gene="kdsB"
                     /locus_tag="PKK81_11760"
     CDS             complement(185..955)
                     /gene="kdsB"
                     /locus_tag="PKK81_11760"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNV33606.1"
                     /translation="MKRSVTVVIPARYGSSRFPGKPLVELLGKPMIQHVYEQAQACRA
                     VDDVLVATDDERIKTAVQRFGGQVVMMTEPYRTGTDRVAAVADSRTGDCFVDLQGDEI
                     LLHPDLITDLVEPFLASEATMGTLKRQIDSDQDLHNPGVVKVTTDREGYALYFSRAPI
                     PLVRDDPKRAAVPGLHFIHLGLYIYTRETLQRLTALPTGTLEEAEKLEQLRALENGMR
                     IRVWETTHGSLRIDSPEDVPGALKQLHSRATGLGITHS"
     gene            complement(957..2030)
                     /gene="rfaE1"
                     /locus_tag="PKK81_11765"
     CDS             complement(957..2030)
                     /gene="rfaE1"
                     /locus_tag="PKK81_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNV33607.1"
                     /translation="MGTMGKVRKSHPVGRTGQTGTSSGQPGEDTTLDHVALSAYVQRF
                     SQAKVLVIGDLILDHYVWGRVSRISPEAPVPVVHVESESLKLGGAANVFNNILALGGQ
                     ADICGVIGADESGRLLLKELGGRRQGRGGVVIDQSRPTTRKSRVVAHNQQIVRYDVER
                     RTELSALLQRRILRYVESRLKELSCLVVSDYAKGVVTASLMTELTRLAGQRKIPIVVD
                     PKVEHFSYYKGVTVITPNHLEATQAAGVQGEDDKAITKAGTILRQRLGCQTVLVTRGE
                     RGMSVYQAHGDHWHIPTRARQVYDVTGAGDTVVGTLALALSTGASMRDAAVLANQAAG
                     VVVGMIGTATVTAAQLTDALGQN"
     gene            complement(1991..2770)
                     /gene="lepB"
                     /locus_tag="PKK81_11770"
     CDS             complement(1991..2770)
                     /gene="lepB"
                     /locus_tag="PKK81_11770"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNV33608.1"
                     /translation="MSLDPNPRPDDSSSTTGPVTPPAPSEQATHAPTVVAVGQPAHKS
                     ILREYAEAIIIAMLLAFAIRVFVVQAFKIPSGSMIPTLLVGDHILVSKLSYGLQWPTD
                     CKIQPGFPPVTCYSSRTLIPFGSIQRGDIIVFRFPEDEDKDFIKRVIGLPGDTIQVRN
                     KVVHINGTPFDDHAFTQHTDPPVHDGRISPRDNFGPVTVPEDAYFVMGDNRDHSLDSR
                     FWGYVRTEKVRGKAFRIYWSWSGQGSWTEWVRWERLGKAIQ"
     gene            complement(2767..4554)
                     /gene="lepA"
                     /locus_tag="PKK81_11775"
     CDS             complement(2767..4554)
                     /gene="lepA"
                     /locus_tag="PKK81_11775"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNV33609.1"
                     /translation="MRNFSIIAHIDHGKSTLADRLLDATGAVTAREAKEQILDAMDLE
                     RERGITIKAHAVAIRYKAQDGKTYLLHLIDTPGHVDFTYEVSRSLAACEGSLLLVDAT
                     QGVQAQTIANVNLAMGNHHTIIPVINKIDLASADVEGTKQQISDVLTLDASDAMLVSA
                     KEGRGVPEVLEAIVKRIPPPSGDPTRPLKALIFDSWFDNYQGVIVLTRIIDGSVRPGM
                     KIKVMSNDRLFEVTEVGQFTPKRTKGTQLMTGEVGYLCANMKEVADVKIGDTLTEAAH
                     PTEQPFPGYKEVKPLVFCGLYSTDTARYEDLRDALLKLRLNDSSFIYEPETSLALGFG
                     FRCGFLGLLHMEIIQERLEREYGLTLITTAPTVIYRILTTKGDVLELNNPAELPEPSS
                     IASFEEPFILATLIAPERYLGTLLQLCQERRGIQRSIHYLDPTRVVISYELPLNEVIL
                     DFYDKLKSKTQGYASLDYELLGYRESELVRLDILLNGEPVDALSFITHKERAYQRGRQ
                     VAEKMKELIPKQMFEIAIQAAIGNKIIARETIGAIKKNVTAKCYGGDISRKRKLWDKQ
                     KEGKKRMKAVGRVEVPQEAFLAILKVGEE"
BASE COUNT          858 a         1321 c         1382 g          995 t
ORIGIN      
        1 tgctggtacg gattcatggt gcccggatcg acgttgatgt agggatcgag cttgaggaac
       61 gtgatcttca gcccgcggct ttccagcaga ttgccgatgg atgcggaagc cagccccttt
      121 ccgagcgacg atacgacacc gccggtcaca aaaatcagtt tgctcatcgt ggcaccttct
      181 ctcgtcatga gtgagtgatg ccgagtccgg ttgcacggct gtgaagttgt ttcagtgcgc
      241 cgggcacgtc ctcggggcta tcgatgcgca aggaaccgtg ggtggtttcc cacacgcgga
      301 tacgcattcc gttttccaag gcgcgcagtt gttcgagttt ttcggcctct tccaaagtcc
      361 ctgtcggcaa ggcagtcaga cgttgcaatg tttcccgggt atagatgtac aagcccaggt
      421 gaatgaaatg cagtcccggc accgcagccc gcttcggatc gtcccgcacc aacggaatcg
      481 gcgcgcggga aaaatacaag gcatagccct cgcgatcggt agtgactttg accacgcccg
      541 ggttgtggag atcctgatcg gaatcgatct gtcgtttcaa ggtgcccatc gtggcctcgc
      601 tggccagaaa cggctcgacc aggtcggtga tgagatcggg atggagcagg atctcgtctc
      661 cctgcaggtc gacgaaacag tcgccggtgc ggctgtcggc gacggcggcg acgcggtcgg
      721 tgccggtgcg atagggctcc gtcatcatca cgacctgtcc gccgaagcgc tgaaccgccg
      781 ttttgatgcg ctcgtcatcc gtggcgacca gaacgtcatc gaccgcgcga caagcctgtg
      841 cctgctcgta gacatgttgg atcatcggtt tgcccagcaa ctcgaccagc ggtttgcccg
      901 ggaatcgtga agaaccgtac cgggctggaa tcaccaccgt caccgaacgt ttcacttcag
      961 ttctgtccca gggcatccgt gagttgcgcg gccgtaaccg tagcggtacc gatcatgccg
     1021 acgacgaccc cggcggcttg gttggcaagc accgctgcgt cacgcatgct tgcgcccgtc
     1081 gagagcgcca gcgcgagtgt tccgacgacg gtatcaccgg caccggtgac atcatagacc
     1141 tgtcgggcgc gcgtggggat atgccaatgg tcgccatgag cctggtacac actcatcccg
     1201 cgttcacccc gcgtcaccaa aacggtttgg catcccaatc gctgccgaag aatcgtaccg
     1261 gcttttgtaa tcgccttgtc gtcctcccct tgcacgccgg cagcctgggt ggcttcgaga
     1321 tgattggggg tgatgacggt gacacctttg taatagctga aatgttccac cttgggatcg
     1381 accacgatgg gaattttgcg ctgccccgcc agtcgagtca actctgtcat gagcgacgcg
     1441 gtgacgaccc ccttcgcata gtccgagacg accaggcagg agagttcttt caagcgcgat
     1501 tccacatacc ggagaatgcg ccgttgcagc agggccgaga gttccgtgcg gcgttcgacg
     1561 tcatagcgaa cgatctgctg attgtgggcg accacccggg attttctggt cgtcggccgg
     1621 ctctggtcga tgaccacgcc gccgcggccc tgccggcgcc cgcccagttc ttttaagagc
     1681 aaccgcccgc tttcgtcggc accgatcacg ccgcagatat ccgcctgccc gccgagtgcg
     1741 agaatgttgt tgaagacgtt ggctgcgccg cccagcttga gggattccga ttcaacgtgg
     1801 acgaccggca cgggcgcttc cggagaaatc cggctcaccc gtccccagac gtagtggtcg
     1861 aggatcaaat caccgatcac cagcactttg gcttgggaga aacgctggac gtaggcgctg
     1921 agtgcgacgt gatcaagcgt cgtgtcctcg cccggttgcc cgctcgacgt cccggtctgt
     1981 ccggttcgcc ctactggatg gcttttccta acctttccca tcgtacccat tccgtccatg
     2041 atccttggcc gctccacgac cagtaaatgc gaaatgcttt gccgcgcact ttttccgtgc
     2101 ggacatagcc ccagaagcga ctatcgagac tatggtcccg attgtctccc atcacgaaat
     2161 acgcgtcctc cggcactgtc acagggccga aattgtcgcg cggactgatg cgcccgtcat
     2221 gcacgggggg atcagtgtgt tgcgtgaagg catggtcgtc gaacggcgtc ccgttgatgt
     2281 gcacgacttt attccgcacc tgaatggtat cgccgggaag accgatgacg cgtttgataa
     2341 agtccttatc ttcgtcttcg ggaaaccgga atacgatgat atcgccgcgc tggatcgatc
     2401 cgaagggaat gagcgtccgt gaggagtagc aggtgaccgg cggaaagccc ggctgaatct
     2461 tgcagtcggt cggccattgc aggccataag acagtttgct gacgagaata tgatcaccca
     2521 ccagcagcgt gggaatcatc gaaccggagg gaatcttgaa ggcctgcacc acgaagacgc
     2581 gaatggcaaa ggcgagcaac atcgcgatga tgatcgcttc tgcgtattcc cgcaggatcg
     2641 acttgtgggc cggctgaccg acggcgacga ccgtgggggc atgggtcgct tgttccgagg
     2701 gtgcgggggg cgttaccggt cccgtcgttg aggagctgtc gtcggggcgc ggattggggt
     2761 cgagactcat tcctcgccca ctttcaggat ggccaggaac gcttcttggg gcacttccac
     2821 ccgtccgacc gctttcatgc gttttttgcc ttctttctgc ttgtcccaga gttttcgctt
     2881 gcgggagatg tcgccgccgt aacactttgc ggtgacattc tttttaatgg cgccgatcgt
     2941 ttcccgcgcg atgatcttgt tgccgatcgc cgcctggatg gcgatttcga acatctgctt
     3001 gggaatcagt tctttcattt tttccgcgac ctgccgcccg cgctgatacg cccgctcttt
     3061 atgcgtgatg aaggacaatg catcgacggg ctcaccattg agcagaatat cgaggcgcac
     3121 cagttcggat tcccgatacc cgaggagttc atagtcgagc gacgcatagc cctgcgtctt
     3181 cgatttcaac ttgtcgtaga aatcgaggat gacttcgttg agcggcagct cgtagctgat
     3241 cacgacgcgg gtgggatcca ggtagtgaat gcttcgctgg ataccgcgcc gctcctgaca
     3301 caattgcagc aaggtgccga gataccgctc cggtgcgatc agcgtcgcca ggatgaacgg
     3361 ttcttcgaac gaggcgatcg aactgggttc aggaagttcc gccgggttat tgagttcgag
     3421 cacatcgcct ttggtcgtca ggatccggta gatgacggtc ggcgcggtgg tgatgagcgt
     3481 caatccgtat tcgcgttcca accgctcctg aataatctcc atatggagca ggccgaggaa
     3541 gccgcaacgg aagccgaacc cgagggcgag cgaggtttcc ggctcataga tgaacgacga
     3601 gtcgttcaag cgcaacttca agagcgcatc ccgcagatct tcgtaacgag cggtgtcggt
     3661 ggaatagagt ccgcaaaaca ccagcggttt gacttcttta tagccgggaa acggctgctc
     3721 ggtcgggtgt gccgcctcgg tcaacgtgtc accgatcttg acgtcggcca cttccttcat
     3781 gttggcgcag aggtaaccga cctccccggt catgagctgg gtgcccttgg tccgtttcgg
     3841 cgtaaactgt ccgacctcgg tgacttcgaa gagccggtcg ttggacatca ccttgatttt
     3901 catcccgggt cgtacggagc cgtcgatgat gcgggtgagg acgatcacgc cctggtaatt
     3961 gtcgaaccag gaatcgaaaa tcagcgcctt gagtggccga gtcggatcgc cggacggtgg
     4021 tgggatgcgt ttgacgatcg cctccagcac ttccggaaca ccgcgtcctt ctttggcgct
     4081 gaccagcatg gcatcgctgg cgtccagggt gagcacatcg gaaatttgct gtttcgtgcc
     4141 ctcgacgtcg gcgctggcga gatcgatctt attgatgacc ggaataatcg tgtggtggtt
     4201 gcccatggcc aggttgacgt tggcgatggt ctgcgcctgc accccctgcg tcgcgtcgac
     4261 gagcagcagc gagccttcac aggccgcgag gctgcgcgac acttcgtacg tgaaatcgac
     4321 gtgaccgggc gtatcgatca aatgcagtaa atacgtcttc ccgtcctgag ccttgtagcg
     4381 gatggccaca gcgtgcgctt tgatcgtgat gccgcgctca cgctccaggt ccatcgcatc
     4441 gaggatctgc tccttcgctt ctcgcgcagt gactgcgccg gttgcgtcga ggagccggtc
     4501 agcgagggtc gatttaccgt gatcgatatg ggcgatgatc gagaaatttc ggatga
//
LOCUS       DAOQRM010000187         4518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_20363_length_4518_cov_14.163119, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000187 DAOQRM010000000
VERSION     DAOQRM010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20363_length_4518_cov_14.163119"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1377)
                     /gene="argJ"
                     /locus_tag="PKK81_11780"
     CDS             complement(166..1377)
                     /gene="argJ"
                     /locus_tag="PKK81_11780"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNV33610.1"
                     /translation="MKIITGGVTAPIGFRAAGMYCGIKKKQKKQLDLALLVSEQEGPV
                     AGVFTTNQVVAAPVVLDRLHLKKGIGRAILVNSGNANACTGPDGMRVAKEMAQLVATA
                     LGSAPHTIFIGSTGVIGQPLPIDCIKNAVPSLLARVTRRGGNEAAQAIMTTDLKPKQV
                     AVRARLGGRMVTVGGMAKGSGMIHPNMATMLGYLTTDAVIGRSALQTALSQAVDQSFN
                     CITVDGDTSTNDTVLCLANGMAGNQTLKPGSADFRRFCAMVETVCRTLALKICWDGEG
                     VTKVVRVEVTRARSTMAAKQIAQTIATSNLVKTALFGGDANWGRVMAALGRAGVPIDP
                     SRVSLQFGDVPMVRNGQGLGRVAEQKLTKVFRAKEFTIRVDLAQGQAEAHMWTTDLSY
                     EYVRINASYRS"
     gene            complement(1433..2506)
                     /gene="argC"
                     /locus_tag="PKK81_11785"
     CDS             complement(1433..2506)
                     /gene="argC"
                     /locus_tag="PKK81_11785"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNV33611.1"
                     /translation="MTSVRVAVAGGSGYTGAELLRLLSQHPNVQLTAVTSEKSAGTPV
                     STVYPHLQGIVPLTFEALAPEALAERADVLFLALPHTKSMGPVASCMKAGKRVIDLSA
                     DFRLKDPRTYETWYQTTHAHPDLIGSAVYGLPELHRSAIAQARLVASPGCYPTAAILQ
                     LAPLIAHGLIVPDTIVIDAKSGVSGAGRSPALPYHFPEAHESLEPYKIGQHRHIPEIE
                     QELSGLAGTRGQAGNGHASAVTIAFTPHLVPMNRGILSTAYARMKGKLDVVELRALYR
                     DFYKGERFVRLLEGAMPNPRHVRGANYCDLAVHTDTRAGWVVTVSAIDNLIKGAAGQA
                     IQAMNLMLGYPEETGLVAPGIYP"
     gene            complement(2619..3011)
                     /gene="rpsI"
                     /locus_tag="PKK81_11790"
     CDS             complement(2619..3011)
                     /gene="rpsI"
                     /locus_tag="PKK81_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNV33612.1"
                     /translation="MAAITQYATGKRKSAIARAWVTAAAGDIVVNEKPLEKAFPRMTL
                     RNVIQFPFELAGVAGKYSVRATVYGGGPAGQAGALRHAISKALVVMSAAFRSPLKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
     gene            complement(3054..3485)
                     /gene="rplM"
                     /locus_tag="PKK81_11795"
     CDS             complement(3054..3485)
                     /gene="rplM"
                     /locus_tag="PKK81_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNV33613.1"
                     /translation="MTKTYLEKPADVKRKWFLVDADGKTLGRLAAKVATLLRGKHKPT
                     FTPHVDTGDHVVIVNAEKIRLTGNKMEDKTYSYHTGYPGGLKTLTAEHIHKKDPTKLL
                     TRAIEGMLPKNPLGNQMAKKLRVYAGSTHPHHAQQLESLSL"
     gene            complement(3536..4024)
                     /locus_tag="PKK81_11800"
     CDS             complement(3536..4024)
                     /locus_tag="PKK81_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNV33614.1"
                     /translation="MDSSKQQHEPSELVALTVKQVLDDGNTDTRIVVLKNDDASVTLP
                     IWVGSAEGNAIRLAMERVVTPRPMSHDLIRSFADHLGVRIERVVITDVKGSTYYASAA
                     FASKGVHRTLDARPSDAIALALRADCPIYATQDVLNRRTAVHLDAWINKLDTKNIEPQ
                     QA"
     gene            complement(4028..>4518)
                     /locus_tag="PKK81_11805"
     CDS             complement(4028..>4518)
                     /locus_tag="PKK81_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNV33615.1"
                     /translation="FLADIGKDTPAVITQMRVKGLIFDPYNNAYIVVLRDEENSDMLP
                     IWVGKSEASAIGLALESVTAPRPMTHDLMKSFLETFDAKIISVVITDLNENTYFATIH
                     LMYEDSEYTVDSRPSDAIALALRTSAPIFASEKVIKKQSSEELEQWLENLKPEDFGKL
                     DT"
BASE COUNT          861 a         1299 c         1323 g         1035 t
ORIGIN      
        1 ccttattctc aggctgcgca gcgtgcgcat cacgctggtg tggatttaaa taagacgtcc
       61 ctgattttga atgcgaaaat gtgaacgatg ggacggacca ttcactgcac gaaaacgccc
      121 gaacgctagc ataatgccca gtattttgca acctcgcgag gtggctcacg atcggtaact
      181 cgcgttgatc cgcacgtatt cgtaggacaa atcggtcgtc cacatgtgcg cctcggcttg
      241 gccctgtgcg aggtcgacac gaatcgtaaa ctccttcgcg cggaagacct tggtcagctt
      301 ctgctcggcg acgcggccca gtccttgacc gttccggacc atcgggacat ccccgaattg
      361 caggctgacc cggctgggat cgatgggtac ccctgcgcgt ccgagggccg ccatcacacg
      421 cccccagttg gcatcaccgc caaagagcgc ggtcttcacc aggttggagg tggcgatcgt
      481 ttgggcgatt tgtttggctg ccatcgtgga tcgagcgcgt gtgacttcca cccgaacgac
      541 cttagtcacg ccttcgccgt cccagcaaat ttttaaggca agggtgcggc agactgtttc
      601 gaccatggcg cagaaccgtc gaaagtccgc agagcctggc ttgagtgtct gattgcctgc
      661 cattccgttc gcaaggcaaa ggacggtgtc gttcgtgctc gtatcgccgt cgacggtaat
      721 acagttaaac gattgatcca ctgcctgact cagtgctgtc tgaagcgccg accggccgat
      781 gaccgcatcg gttgtgagat agcccagcat ggtggccata ttgggatgga tcatcccgga
      841 acccttggcc attcctccca ccgtcaccat ccgccctccg aggcgggcgc gcaccgccac
      901 ctgtttgggt ttgaggtccg tggtcatgat ggcctgggcc gcttcgtttc cgccgcggcg
      961 cgtgacgcga gcgaggaggg acgggacggc gttcttgatg cagtcgatcg gaagtggctg
     1021 gccgatcacg ccggtcgatc cgatgaagat agtatggggc gcactgccga gggccgtggc
     1081 aaccagctgc gccatctcct tggccactcg cattccgtcg ggtccggtgc aggcgttggc
     1141 gttcccgctg ttgacgagga tggcccggcc gatgcccttc ttcagatgca ggcgatcgag
     1201 cacaactgga gcagccacga cctgattggt ggtgaacacg ccggcgacgg gtccctcctg
     1261 ctcggagacc agcagggcca agtcgagttg tttcttctgc tttttcttga tgccgcaata
     1321 catcccggcg gcccgaaagc cgatcggtgc agtgacgcct ccagtgatga tcttcatagt
     1381 gatcctgtgt ggtgagaaat ggtccgtagc gcggagtcgc ccaggactca aattatggat
     1441 agatacccgg cgccaccagg cccgtctctt ccggatagcc gagcatgaga ttcatagcct
     1501 gaatcgcttg tcctgctgct cccttgatca ggttgtcgat ggccgacacg gtcacgaccc
     1561 agccggctcg cgtatcggtg tggactgcga gatcgcagta gttggcgcct cgcacatgtc
     1621 ggggattcgg catcgcgcct tcgagcagtc gcacgaatcg ttctcccttg tagaagtccc
     1681 gatagagcgc ccgcagttcg acgacatcga gcttaccctt catgcgcgca taggcggtgc
     1741 tcaagatgcc tcgattcatc ggaaccaggt gcggcgtgaa tgcaatggtg acagccgaag
     1801 cgtgtccgtt gccggcctgt ccacgcgttc ctgccagccc gctcagttcc tgttcgattt
     1861 ccgggatgtg tcgatgctgg ccgatcttat agggctccag tgattcgtgt gcttcgggga
     1921 agtggtaggg cagggcagga cttcttcctg cgcccgacac gccagatttt gcatcaatca
     1981 caatggtgtc cggcacgatg aggccatggg cgatcaacgg cgccagttgc agaatcgcgg
     2041 cggtcgggta gcagccgggg gaggcgacaa gtcgggcctg tgcgatggcg gatcgatgca
     2101 gttccggcag gccatagacg gcgcttccga tcaggtcggg atgcgcgtgt gtggtctgat
     2161 accaggtttc gtatgtgcga ggatctttga gtctgaagtc tgcgctcagg tcgatcacgc
     2221 gcttgccggc tttcatgcaa ctcgctaccg ggcccatgga ttttgtatgg ggaagggcga
     2281 ggaagagtac atcggcgcgt tctgccaggg cctcgggcgc cagggcttcg aaggtcagcg
     2341 gcacgatgcc ctgcaaatgg ggatacacgg tggagacggg ggttcctgct gacttctcgg
     2401 aagtcaccgc agtcaattgc acgttggggt gttgcgacag cagccgtagc aactccgctc
     2461 cggtgtatcc gctgccccct gccactgcta ctcgtaccga cgtcatgtca cccctcccgg
     2521 cctatcgcga attgttgctc gatcagttca gcgcacacat aaaaaaaggg aaggctgcgg
     2581 agccttccct tttgacacct tgtcgctcca gtgtttcttt atcgtttcga gtattggaag
     2641 cgctttctgg cgcccttctg gccgtacttt ttgcgctctt tcacgcggga gtcacgggtc
     2701 agaagacctt ccttcttgag cgggcttcgg aatgcagcgc tcatgacgac cagcgccttc
     2761 gagatagcat gccgcaaggc tccggcctgg cccgcaggcc caccaccata gacggtggcc
     2821 cggacggaat acttaccggc cacgccagcc agttcaaacg gaaattgaat gacattccgc
     2881 aaggtcatgc gaggaaacgc tttttccagc ggcttctcgt tgacgacgat atcaccagca
     2941 gcggcggtca cccatgcacg agcgatcgcg ctctttctct tcccagttgc gtactgcgta
     3001 attgctgcca tatcaggcgg cctccttcac agatcgaatc cgaacaataa tgatcacagc
     3061 gaaagtgatt ccagttgctg cgcgtgatgt ggatgggttg aacctgcgta cacgcgcagt
     3121 ttcttggcca tctgattccc aaggggattc ttgggcaaca tgccttcgat ggcgcgcgtc
     3181 aagagcttcg tcgggtcctt cttgtggata tgttcggcgg tgagagtctt gagtccgcct
     3241 ggatatcccg tgtggtacga ataggtcttg tcttccatct tgttgccggt caaccggatc
     3301 ttctccgcat tgacgatgac gacatggtcg ccggtatcca cgtggggggt aaacgtcggt
     3361 ttatgcttgc ctcgaagcaa ggtggccacc ttcgccgcaa ggcgaccgag cgtcttgccg
     3421 tcggcatcca ccaagaacca tttccgtttc acatcagctg gcttctcaag atacgttttt
     3481 gtcatggtct ccatctactc ctgcacgaaa ggattctaat gtgaggtatc aggcctcacg
     3541 cctgttgggg ttcgatattc ttagtgtcga gtttattgat ccacgcatcc aggtgaacgg
     3601 ccgtgcgccg gttgagcaca tcttgtgtgg cataaatcgg gcaatctgcg cgaagtgcga
     3661 gggcgatggc gtcgcttggc ctggcatcca gggtgcgatg cacgcctttc gatgcaaagg
     3721 ccgcgctggc atagtacgta ctgcctttca catccgtaat cacaacccgc tcaatccgca
     3781 caccaagatg atccgcaaag ctccgaatga ggtcatggct cataggacgg ggagtgacca
     3841 cccgttccat ggcaagacga atggcgttac cctcggccga tccaacccag ataggcagtg
     3901 tgacgctcgc gtcgtcattt ttcagcacga cgatgcgggt gtcggtattg ccgtcgtcca
     3961 gcacctgctt gacggtcaga gcgacgagct ccgagggctc atgctgctgc ttcgacgagt
     4021 ccatgaacta ggtatccagt tttccgaaat cttccggctt caaattttca agccactgtt
     4081 caagttcttc ggacgactgt ttcttaataa ccttctcgct ggcaaaaatc ggcgcgctgg
     4141 ttcgcagcgc cagcgcgatg gcatcgctgg ggcgagaatc gaccgtatat tccgagtctt
     4201 cgtacatcag gtgaatcgtg gcgaaatagg tgttctcgtt caaatctgta atcaccacgc
     4261 tgataatttt cgcatcgaac gtttccagga acgacttcat gaggtcgtgg gtcatgggac
     4321 gcggtgcggt gacactctcc agcgcaaggc cgatggcact ggcttctgat tttccgaccc
     4381 agatcgggag catgtccgaa ttctcttcgt cccgcaacac cactatatag gcgttgttgt
     4441 aggggtcgaa gatcagaccc tttacccgca tttgtgtaat cacggcggga gtatccttcc
     4501 cgatgtcggc caaaaacc
//
LOCUS       DAOQRM010000188         4485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_20593_length_4485_cov_12.939503, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000188 DAOQRM010000000
VERSION     DAOQRM010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20593_length_4485_cov_12.939503"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            431..664
                     /locus_tag="PKK81_11810"
     CDS             431..664
                     /locus_tag="PKK81_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33616.1"
                     /translation="MEKPIMNPELPLPEIFKWRVPINWDPVPWWFFERANLPKEALSQ
                     LALIQLENQRAIMQQNMKAIEQSMEMVKKFGGK"
     gene            823..2478
                     /locus_tag="PKK81_11815"
     CDS             823..2478
                     /locus_tag="PKK81_11815"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV33617.1"
                     /translation="MRSLTLLDERGAMPSLQALSEQDATRPVVLIGFQKQGNLGIGYL
                     AATLMRRGHRVKVLDFEDAAEQILATIEAEAPVVVGFSLIFQFYLPRFRALMQYLRNH
                     GVRCHFTIGGHFPSLSHERTLELIPEVDSVVRFEGEVTLLELVESLIHDQDWRRIDGL
                     AYRRDGRFVSNPLRHLLDDLDVLPFPYRQYEPTAILGQRTMPILASRGCSRTCSFCSI
                     HMFYRAAPGRVVRIRKVTEVVREMKDLYQNRGITLFLFQDDDFPLFGPSWRRWALSLV
                     DELYRQDLVGKVIWKISCRADAVEPELFATLRDAGLYLVYMGLESGSDDGLDVLHKEI
                     TVAENLRAVETLKSLGLMWEFGFMLFDPSSTFESIETNVGFLRRIVDDGSVAAVFCKM
                     LPYDGTPIKETLMAQGRFNGDVCNPDYDFLDPRIARCYEAIGRTVGEWVRGSDCVAAM
                     INHAWHEVFVMERLFEPGADLVAYKTALSAVTKFSNNILFSVVEEICDGFREGGGECQ
                     QYSGLGQIREQILATLVQQRNRFVYENQSWLLKGISKQTLVTA"
     assembly_gap    2552..2651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2652..3879
                     /locus_tag="PKK81_11820"
     CDS             <2652..3879
                     /locus_tag="PKK81_11820"
                     /inference="COORDINATES: protein motif:HMM:NF019913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33618.1"
                     /translation="GIGHYRHYRSGSIYWSPASGAHEVHGLIRDRWAKLGWERSFLGY
                     PLTDETTTPDRIGRYNHFQGGSIYWTPATGAHEVHGAIRGRWASLGWERSFLGYPLTD
                     ETTTPDRIGRYNHFQGGSVYWTPATGAHEVHGAIRGKWASLGWERSFLGYPLTDETTT
                     PDGVGRYNHFQGGSVYWTPATGAHEVHGAIRALWASMGWERSFLGYPTSDELSTEDST
                     GRYSEFQHGSIYWSPGTGALACRETVRLHVKCLTAPTRFTINQMISNMRLTYATAQVG
                     LKYVSFEVLNLPALNDIDVGACTMGTVTAEQTQLFANRNNAAAKDVIAYFVRSTQPPF
                     NGCASHPANRPGAVVASGASAWTLAHEIGHVLGLSHVSDNNRLMTGLGTDNITNPPPD
                     IIASEKTTMLASSFTT"
     gene            3895..4353
                     /locus_tag="PKK81_11825"
     CDS             3895..4353
                     /locus_tag="PKK81_11825"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV33619.1"
                     /translation="MPITMQQVLAEIDKDEPNYEALALLGPEALPHLQLIAEADDPLK
                     SSKAVYAASLIGGAGAVEVLRKAADHHDPQVRIAVAQGLQNLAAVAPTDLVMKSLNDH
                     DSGVRKLALMTAGKLNRTEFKQRITAIAQGDPEEHLRKAASVAAKKLEVR"
BASE COUNT          931 a         1131 c         1374 g          949 t
ORIGIN      
        1 cccgattgat ttggatctgt ccgccccatc gactcgaagc ctacaacaac cagcagctca
       61 cctggaatca ccttgacggc ttgcgagagg cacatcgtcg gcgaatccgg aacgtcccaa
      121 tcgaagtcgt cggtgtgaca ccctgccatt aggccttctc ttaaacaacc attttcctga
      181 ttcgtctacg tatcaatttg gatacgtgag tatcgcatcg tatacacgaa ggcctgatcc
      241 ttttccgact gcagaccttc caaatcattg gaagtaggta aatgacatga ccgctgcaat
      301 gtggccgata tcttgcttgt tcctctgctg gtgacgacac atgtcgtaac tcattgtgtg
      361 ctgcgtggcc ggggggacac cgcgatcacg acgatcgcaa tactgagcgt actcaccaaa
      421 ggaggaccga atggaaaagc ccatcatgaa tccggaactg ccattgccgg agatattcaa
      481 gtggcgagtg ccgatcaatt gggatcctgt gccttggtgg ttcttcgaac gtgcaaacct
      541 tccgaaggaa gcgctgagtc agctggcgtt gattcagctg gagaatcagc gggcgatcat
      601 gcagcagaac atgaaagcga ttgagcagtc gatggaaatg gtcaagaagt tcggcggcaa
      661 atagctacgt gcaagagcct cagtggaagg gagtggacgc cacccccggc cctatgccgc
      721 tccttttcag aggatttcgc tgtgcaggaa gcgggttcga catgacaatg atcgggcctg
      781 gtgttgccac gcattgttgc ggccattcct tgggaggagg ctatgagatc actcactctg
      841 ctggacgaga gaggggccat gccctctctg caggccttga gcgaacagga cgcgaccaga
      901 cccgtggtct tgatcggatt tcagaagcag ggcaaccttg gaatcgggta tctggccgca
      961 acgctgatgc gacgagggca tcgtgtgaag gtgctcgatt tcgaagatgc ggcggagcag
     1021 atcttggcga cgattgaagc tgaagccccg gtggtggtcg ggttttcgct catctttcaa
     1081 ttttatctgc ctcgcttccg tgcgttgatg caatacctgc ggaaccatgg cgtccggtgt
     1141 catttcacaa tcggcgggca ctttccaagt ctgagtcacg agcgaacgct tgagttgatt
     1201 ccagaggtgg acagcgtggt tcgattcgaa ggggaggtga ccctgctcga gctcgtggaa
     1261 tcgctcattc atgaccagga ttggcgcagg atcgatggcc tcgcgtatcg tcgggacggg
     1321 cgattcgtgt caaatcccct tcggcatctc ctggacgacc tcgatgtgtt gccctttccc
     1381 taccggcagt atgagccgac ggcgatcctc ggtcagcgaa caatgcccat cctggcgtca
     1441 cgcggctgtt cccggacctg ttccttttgt tccattcata tgttttatcg ggccgctccg
     1501 ggacgggtcg tcaggattcg gaaagtaacc gaagtggtcc gcgaaatgaa ggatctttat
     1561 cagaaccggg ggattaccct gttcctgttc caagatgatg atttccccct cttcggcccg
     1621 tcgtggcgtc ggtgggcgct cagtctggtc gatgaactgt atcgccagga cctggtcgga
     1681 aaagtcatct ggaaaatcag ttgccgggcc gatgcggttg agccggagtt gtttgcgacc
     1741 ttgcgggatg ccgggttgta cctggtgtac atggggctgg agtctggatc ggacgatgga
     1801 ctcgatgtgc ttcacaagga aatcaccgtg gcggaaaatc tgcgggccgt tgagacgctg
     1861 aagtcgctgg gcctcatgtg ggaattcggc ttcatgctgt ttgatccatc cagcacgttc
     1921 gaatcgattg aaacgaatgt ggggtttttg cgccggattg tcgacgatgg gagtgtcgcg
     1981 gcggtgttct gcaaaatgtt gccctatgac ggcacgccta tcaaggagac gctgatggcc
     2041 cagggacggt tcaacggcga cgtgtgtaac ccggactacg attttctcga tccccggatt
     2101 gcgcgttgct acgaggcaat aggacgaaca gtcggcgaat gggtcagggg atcggattgt
     2161 gtggccgcga tgatcaatca tgcgtggcat gaggtctttg tgatggagcg gcttttcgag
     2221 cccggtgcag accttgtcgc atacaaaact gcactgagtg ccgtgaccaa attcagcaac
     2281 aacatattgt tctcagtggt agaagaaatt tgcgacggtt ttcgggaggg cgggggtgag
     2341 tgtcaacagt actcaggcct gggacagatc cgggagcaga ttctggccac cttggtacag
     2401 cagcgaaacc ggtttgttta tgagaaccag tcctggttgt tgaaagggat ctcgaagcaa
     2461 acgcttgtga ctgcctaagt cgacagcatc acctgcgcgg ttcagacggc ccgatggttc
     2521 tgaggaacct cggacaggaa tgcgtcagcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn ncgggatcgg ccattaccgg cattaccgaa gcggctccat ctattggtcg
     2701 ccggcatccg gagctcatga agtccacgga ctcatccgtg acagatgggc gaaattgggg
     2761 tgggaacgga gcttcctggg ttacccgttg acggatgaga cgacgacgcc ggatcgcatc
     2821 ggccggtaca accattttca aggcgggtcg atctattgga cgccggcgac cggtgcccat
     2881 gaggtgcatg gcgccattcg gggtaggtgg gcgagtttgg ggtgggaacg gagcttcctg
     2941 ggctatccat tgacggatga aacgacgacg ccggatcgca tcggccggta caatcatttt
     3001 caaggcgggt cagtctattg gacgcccgcg accggtgccc atgaggtgca tggcgccatt
     3061 cggggtaagt gggcgagttt ggggtgggaa cgcagctttc tgggctaccc gttgacggat
     3121 gagacgacga cgccggatgg ggtcgggcgg tacaatcatt ttcaaggcgg gtcagtctat
     3181 tggacgccgg cgaccggtgc ccatgaggtg catggcgcca ttcgggctct atgggcgagc
     3241 atgggatggg agcgcagttt tctgggatac cccaccagcg atgaattgag tacggaagac
     3301 agtaccgggc gatatagcga attccagcat ggatctattt actggagccc ggggacaggg
     3361 gcgctggcct gccgtgaaac ggtgcgattg cacgtcaagt gtctcacggc gcccactcga
     3421 tttacgatta accagatgat ttccaatatg cgtctgacct atgcgaccgc gcaagtcggc
     3481 ctcaaatatg tgtcgtttga agtgctgaat ctgccggcgc tgaacgatat tgatgtcggt
     3541 gcctgtacga tggggacggt gaccgcggaa caaacacagc tatttgccaa ccggaataac
     3601 gcagcggcga aggatgtgat tgcctacttc gttcgcagca cgcagccgcc gttcaacggc
     3661 tgcgccagtc atccggccaa ccggcccggg gccgtggtcg ccagcggggc cagcgcatgg
     3721 accttagcgc atgaaatcgg ccacgtcttg gggctgtcgc acgtcagtga taacaaccgg
     3781 ctgatgaccg gactgggaac cgacaatatt actaaccctc cgccggacat tattgcctca
     3841 gagaagacga cgatgttggc gagctccttt acgacttagc cgggagacaa gaacatgcca
     3901 atcacaatgc agcaggtatt agctgaaatc gacaaagacg agcccaatta cgaagcgctg
     3961 gctctgctgg ggccggaggc gctgccgcac ttacaactga ttgccgaggc cgacgatccg
     4021 ctgaaatcgt ccaaggccgt ctacgctgcg agcttgatcg gcggagcagg cgcggttgag
     4081 gtattaagga aggccgccga tcaccatgat ccgcaggttc gtattgcggt cgcccaaggg
     4141 ctgcagaatc tggccgcggt tgctcccacc gacttggtca tgaaatcgct caatgaccac
     4201 gattcgggtg tccggaagct ggcgctgatg acggcaggaa agttgaatcg gactgagttc
     4261 aagcagcgga ttacggccat cgcgcagggc gatcctgaag agcatctgcg aaaggctgcg
     4321 tcagtggcgg caaagaagtt ggaggttcgg taggcagacc atcctctcct ccgccctgcg
     4381 tgatgcgggg tgggggagag gcaacaacgc gtatcccaaa gggccttggc caggaggaga
     4441 atctgtcccg ctattgtgcg cggcgcaact ccccgtagag tgggg
//
LOCUS       DAOQRM010000189         4472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_20680_length_4472_cov_15.902196, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000189 DAOQRM010000000
VERSION     DAOQRM010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20680_length_4472_cov_15.902196"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKK81_11830"
     CDS             complement(<1..377)
                     /locus_tag="PKK81_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33620.1"
                     /translation="MAANRANRILSVAGLTLLLTSPAAAQSVSSVRGLGGGVAPLFGL
                     VGPGNLYIDNQGTQGYIYNFGNNFESYNFRNPATGQAWSGALMTLGPQLSVGLIQGAN
                     QAGSPVVFPPVPREAGPLSPVQSG"
     gene            483..2033
                     /locus_tag="PKK81_11835"
     CDS             483..2033
                     /locus_tag="PKK81_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV33621.1"
                     /translation="MTNAILRGCLFIGGAAALLLWTAPDSFGLEVNKPVPTERREAMS
                     LADAVLKALQNNLDISIGRQTRESRLADIVIEQAKFDPTVSLNGQYNRQVSPLNRPIL
                     GFTGANLQEITKFDQNSHSLTADITQNLPTGANYDLNYSPQRSYVSGPNTFLFNPAWT
                     GGLALTVTQPLLKNFGTDINKTFISIAQNNATVEQHVFLDRVLTVIASVEQTFWEMVF
                     ANENLKVAQAALKAAEELLASNRAKAKAGVMSIVDVLQAEAAVASRVEQILVAEKSIR
                     DQEDQLRRLLNPVEEELRQDLRLIPTDPPVTSLEAISLQEAIDIAMERRPEVLQAGKN
                     VESSDLNVKFAKNQLLPTLSVQGTMGLSGLGADYGDATRRNLGGDFYNYGAGLVLSYP
                     IGNRSAYSTYNKRQLESRNAQSSLQSVRQQVIVGVREAVRRVHTDFKRIETTRSARIM
                     AEKQLQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRAVLDYNKSLSNLARNKATTL
                     ERYSLRLD"
     gene            2041..2724
                     /locus_tag="PKK81_11840"
     CDS             2041..2724
                     /locus_tag="PKK81_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33622.1"
                     /translation="MIKPDDASSTHATQAGERGAALVLLPLTATILFYSSPTTLQQHN
                     LFQFLPQACAYAAFIAWSTINGSVARRIGLARPLVPQGLRWGLLTGLTLGPINVLVIL
                     YLVPILGGDYRFLADTPHAKIPLLIMVPWFILLIATMVELNFRGFLLGRLLALGLPAP
                     IAVPASALLFAFDPFLVATFQHLHWIAVWDGLVWGTLWVMLRNLYAPIVAHAVEVIVL
                     YSVMRAILT"
     gene            2815..3834
                     /locus_tag="PKK81_11845"
     CDS             2815..3834
                     /locus_tag="PKK81_11845"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33623.1"
                     /translation="MNPSFSGHLVNDVFGDPGLFVEVRWSKRAFLFDLGHNDALGPTR
                     LLRAGDIFISHTHMDHFIGFDALLRVALGRGKTLRLYGPPGLIANVQGKLHGYTWNLV
                     DGYPLTITVQEFHEQTIRRATFLATDGFQRHDAPEGAPINRQAGHRFSVLADPMFTVE
                     AVALNHRIPSLAYSLQEQFHVNVNKERLHEAGLPVGYWLKEVKQYLWQGKSDDFRFQA
                     TLYHEHHKEEREFVLGEVRERFITISRGQKLAYVVDARYDEENEAKIVELAQGADIFY
                     CEAPYLDQDADKARDRYHLTARQAGVMAKKAGVRELVVFHFSPRYTGLGHRIEAEAQQ
                     AFRGS"
     gene            3847..4185
                     /locus_tag="PKK81_11850"
     CDS             3847..4185
                     /locus_tag="PKK81_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HNV33624.1"
                     /translation="MGEWAKYGLYFLLGGTIVSISTYLGSQGRSFLAAFASTFPAMTG
                     ATFILIYLNGGSEHLVTYAKNLLWFVPPWLVYVGCMIYGVERVGFWLSMAGSMVLYMC
                     CVGLVKLLAR"
     gene            4246..>4472
                     /locus_tag="PKK81_11855"
     CDS             4246..>4472
                     /locus_tag="PKK81_11855"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNV33625.1"
                     /translation="MTKTMSLKQARSRFSSLVDNADRLSERVIVTKNGTPKAVVMGAE
                     EFETWVETLELLSNPQAVKALEQGLKEAKAG"
BASE COUNT          945 a         1431 c         1228 g          868 t
ORIGIN      
        1 cccgactgaa cgggagaaag cggccccgcc tcacgcggga cgggaggaaa gaccacgggc
       61 gatccagctt gattcgcacc ctgaatcagc cccaccgaga gttgcggacc gagcgtcatc
      121 agggcgccgc tccaggcctg cccggtcgcc ggattgcgga agttatacga ttcgaagtta
      181 ttcccgaagt tatatatgta cccttgcgtc ccctggttat cgatatagag gtttcccggc
      241 cccaccagac caaacagcgg cgcgacccct ccccccagac ctcgtacact cgagacggat
      301 tgcgccgctg ccggggaagt cagcagcagc gtcagaccag ccaccgacag gatcctgttc
      361 gcgcgattcg cagccaccga cacgctcctc agcatggaca ctgacttctg tttatggtac
      421 cacgccagca tcattttgaa ttcagtatgg cccaacgcag acctcgcatc aaggagtttg
      481 ttatgacgaa tgcaatcctc cgggggtgcc tgttcatcgg gggcgcagcg gcgctcctcc
      541 tctggacggc acccgattcc ttcggcctgg aagtgaacaa gccggtcccg acggaacgcc
      601 gggaggccat gtcgctcgcc gacgctgtcc tgaaagcctt gcagaacaat ctcgatatca
      661 gtatcggtcg ccaaacgaga gagagccgcc tggccgacat cgtgatcgag caggcgaaat
      721 tcgatccgac cgtcagtttg aacgggcaat acaaccgtca ggtctcaccg ctcaaccggc
      781 cgatcctggg attcaccggg gcaaacctgc aggagatcac caaattcgac cagaactccc
      841 actcgctcac cgccgacatc acacagaatc ttccgaccgg cgccaactac gatttgaact
      901 acagtcccca acgcagctac gtgagcggcc ccaacacctt cctcttcaac ccggcctgga
      961 ccggcggact cgccttgacg gtcacccaac cgttgttgaa gaacttcgga accgacatca
     1021 acaagacgtt catctcgatc gcgcagaaca atgccacggt cgagcaacat gtctttctcg
     1081 accgtgtgct caccgtcatc gccagcgttg aacagacatt ttgggagatg gtgttcgcga
     1141 atgaaaatct caaagtcgcg caagcggcgc tcaaggcggc ggaagaactg ctggccagca
     1201 atcgcgccaa agccaaagcc ggggtcatgt cgatcgtcga tgtgctgcag gccgaagccg
     1261 ccgtcgcctc gcgagtcgag cagatcctcg tcgcggaaaa atcgattcgc gatcaggagg
     1321 accagttgcg ccgcctcctg aaccccgtcg aggaggaact ccggcaggac ttgcgcctga
     1381 tccccaccga tccacctgtc acatcactgg aggcgatcag cttgcaagaa gccatcgata
     1441 tcgccatgga gcgccggccg gaggtgttac aagcagggaa gaatgtggag agcagcgacc
     1501 tgaatgtgaa gttcgccaag aaccaactcc tcccgaccct gtccgttcag ggcacgatgg
     1561 gactgtcggg actcggcgca gactacggcg atgccacgag acggaattta ggcggtgact
     1621 tctacaacta cggcgcgggc ctggtcctca gttaccccat cgggaaccgg tcggcgtaca
     1681 gcacctacaa caaacggcaa ttggaatccc gtaacgccca gtcgtcgctc cagagcgttc
     1741 gccagcaggt gatcgtcggt gtccgggaag cggtccgtcg cgtccacacc gatttcaagc
     1801 gcatcgaaac cacccgctcc gcccgtatca tggccgagaa acaactacag gccgaacagg
     1861 aacggctgaa ggtcggtctc agtacgacgc gcttcgtgct cgactttcag cgcgacctgg
     1921 ccaccgccca aggcaacgag cttcgagcgg tgttggatta caacaagtcg ctgtcgaatc
     1981 tggcccgtaa caaggccacc acccttgagc gatacagtct gcggctcgac taacctgtcc
     2041 atgatcaagc ctgacgacgc gtcctcgact catgcgactc aggccggcga acggggagca
     2101 gccctcgtgc tgctccccct gactgccaca atcctgtttt acagctcacc gaccacactc
     2161 caacaacaca acctctttca gtttctcccc caggcctgtg cctatgccgc cttcattgcc
     2221 tggagcacga tcaatggatc cgtcgcccgt cgaatcgggc tcgctcgccc gcttgtcccg
     2281 cagggcctcc gttggggcct gctcaccggc ctgacactcg gaccgatcaa tgtgctggtc
     2341 atcctctacc tcgttcccat tcttggggga gactacagat ttctcgccga taccccgcac
     2401 gccaaaattc ccctgctgat catggtgccc tggttcatcc tgctcatcgc caccatggtc
     2461 gagttgaact ttcgcggctt tctcctgggg cggctcctcg ccctggggct cccagctccg
     2521 attgccgtgc cggcgagcgc cctgctgttt gcgttcgatc cctttctggt ggcaaccttt
     2581 caacatctgc attggattgc cgtctgggac ggcttggtgt ggggtaccct atgggtcatg
     2641 ctcagaaacc tctacgcgcc gatcgtggcc cacgccgtcg aagtcatcgt cctctacagt
     2701 gtcatgcgag cgatattgac ctgatattgt cctcttccgg caaccagaag aacctaaagt
     2761 aaccgttgca ctcataatgg attcaccgct cctgacaaag taatacagca ctccatgaac
     2821 ccttccttct caggtcacct cgtcaatgat gtcttcggcg atcccggcct gttcgtggaa
     2881 gtgcgctggt ccaaacgcgc tttcctcttc gatctgggcc acaacgatgc gctcgggcca
     2941 acccgcctgc tgcgcgccgg cgacatcttc atctcgcaca cccacatgga ccactttatc
     3001 gggttcgatg cgctgctccg cgtcgccctc gggcgcggca agactctgcg gctctacgga
     3061 ccgcccggcc tcatcgccaa tgttcaagga aaacttcatg gctacacctg gaacctcgtg
     3121 gatgggtatc cgctcaccat caccgtgcag gaattccacg agcagacaat ccggcgcgcc
     3181 accttcctcg caaccgacgg attccagcgc cacgacgctc cggagggagc ccccatcaat
     3241 cggcaagccg gccatcgttt ttccgtgctc gcagatccca tgttcacggt cgaagcggtc
     3301 gcactcaatc accgcattcc ttccctggcc tactcgctgc aggaacagtt tcatgtgaac
     3361 gtcaacaagg agcggctgca cgaagccggg ctaccggtcg gctattggct gaaggaagtg
     3421 aaacagtatc tctggcaggg caagtcggac gactttcggt ttcaggcgac gctctaccat
     3481 gaacatcaca aggaggaacg cgagtttgtc ctgggcgagg tccgtgagcg attcatcacc
     3541 atcagccgcg gccagaaact cgcctacgtg gtggacgcgc gatacgacga agaaaatgaa
     3601 gccaagatcg tggagcttgc gcaaggcgcc gacatctttt actgtgaggc cccctacctg
     3661 gatcaggatg cggacaaggc gagggatcgc taccacctca ccgcgcgcca ggccggagtc
     3721 atggcgaaga aagccggtgt gcgcgagctg gtcgtgttcc acttctcccc gcgctacacg
     3781 ggactaggcc accggatcga ggcggaagcg caacaggcat ttcgaggcag ctgaaggagg
     3841 tcgcggatgg gcgagtgggc gaagtacgga ttgtacttct tgttgggcgg caccattgtc
     3901 agcatctcaa cctatctggg atcacaaggc cggtcgtttc tcgccgcctt cgccagtacc
     3961 tttccggcga tgaccggcgc gacgttcatc ctcatctatc tcaacggcgg cagcgaacac
     4021 ctcgtgacct acgccaagaa cctgctctgg tttgtgccgc cctggctcgt ctacgtcggc
     4081 tgcatgatct acggcgtgga gcgcgtcggc ttctggctct ccatggccgg ctccatggtc
     4141 ctctacatgt gctgcgtggg gctggtaaag ctgctggcgc ggtagggctg ataccttgtc
     4201 ggacgagaca caattgtgta cacgccacat caacgaggca tgacgatgac aaaaaccatg
     4261 tcgctcaagc aggcccggtc cagattctcc tccttggtgg acaatgccga tcgtttgtcc
     4321 gagcgggtaa tcgtgacaaa aaatggaact cctaaagcgg tggtcatggg ggcggaagaa
     4381 tttgaaacct gggttgaaac attggagctg ctctcaaatc cccaagcggt caaagctctt
     4441 gagcaagggc tgaaagaagc gaaggcaggg aa
//
LOCUS       DAOQRM010000190         4456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_20803_length_4456_cov_20.124517, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000190 DAOQRM010000000
VERSION     DAOQRM010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20803_length_4456_cov_20.124517"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PKK81_11860"
     CDS             complement(<1..923)
                     /locus_tag="PKK81_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNV33626.1"
                     /translation="MNRDDSSEQPRSPDEPISLSSDESDEDEWEEERPQFSAWMLPAL
                     LFLLTVFTVLWAGAYQTNTNPLVGPWNFLVDDPGSLWRGVPFAATLLGILVTHELGHY
                     VLSRIHGVPTSLPLFVPGLPHFVGTFGAIIRMRAPLTDRRALFDIGVAGPIAGFVVAV
                     IALVVGLRLSTVVPIQTSYGMHLGEPLLLQFASWVVIGPLAPTADVILHPIGFAAWFG
                     LFITSLNLLPIGQLDGGHVAYALLGDRQRSVAVALVPILMVFGWLGWKGWFLWVGLAG
                     MMGLAHPPVQNPHRELGGIRVLVGWIALAIFV"
     gene            complement(1143..1979)
                     /locus_tag="PKK81_11865"
     CDS             complement(1143..1979)
                     /locus_tag="PKK81_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV33627.1"
                     /translation="MPLEDELSDILKKARNGQQRSMADVARASGLSEVEVSELERGQS
                     PRSREQVQAVARALGLRTEPLTQVVDGWTPEALPSSVPHVETVFGSIGGYEVKGYVVH
                     DRGEAVVVDTAYNASGMLALLAQRKLRLRAICLTHGHSDHAEGIEAILRAYPVPVYLG
                     PDDFDLLSWRPSPQVLHVPQGGETLPVGGLTIRFMPTPGHTPGGVCYRLEQAGAPICF
                     VGDTLFAGSIGRSNPAGLYETHLESVRKQVLTLAPDTRLFPGHGPATTVREELLHNPF
                     YS"
     gene            complement(2013..3461)
                     /locus_tag="PKK81_11870"
     CDS             complement(2013..3461)
                     /locus_tag="PKK81_11870"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV33628.1"
                     /translation="MRTDRGWVSVLAMVFMIVLQATVGLAADPTSNDPRTMRFPTVEF
                     NPPDAERVVLENGMVVYLLEDHELPLVTINVTLRTGSWLDPADKVGLAGMTGAVMRTG
                     GSAGMSPDEVDEELEQLAASMSIGFAKESGSASLDVLKKDLKRGLQIFADLLRRPAFE
                     QGRVELAKLQALEGIRRRQDSPGSIVGREFAKLLYGPTHPSARETSVRSIEAITREDL
                     VAFHQRTVHPNGIILGVTGDFEKADMLALLRAAFGDWAKGNVPAVTIPAVSEPDAKTG
                     LVRFVNKDTSQTHLRVGHLTIKETDPDYVAVAIANDILGGSSFRSRLFNDVRTKRGLA
                     YSVGSGLRASVYDEGVWLMRAETKLSSTQEVVNRFVANMERMRNEPVTDTELEEAKEA
                     YVNSFVFSFTSASSIVGRLMDLEYDGLPKDWLQQIRDRVVKLTKEDIQRAAKAHFNPE
                     RLRILAVGSGEALSKVLASFGEVKEITLVPES"
     gene            complement(3518..>4456)
                     /locus_tag="PKK81_11875"
     CDS             complement(3518..>4456)
                     /locus_tag="PKK81_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV33629.1"
                     /translation="EFYKERGVVMEERRLRTDDSPNGLLYETFTSTAFQAHQYGVPTI
                     GWGSDILSLTPAATEAFFKTYYGPNNATVAIVGDINPKEVIALIEQTFGKIPAAPPVP
                     SLVTEEPPQRGERRVEIEFDAEPALAIGYHKPTIGHPDDFVFDVIDEVLTEGVTSRLY
                     STLVRDKRLAASVLSDTNYPGVRAPNLFVIAATPLAPHTTPEVESAIYEELDRLKAEP
                     ISTKEFERVLNGLDADLVRSLRSNSGLASQLAFYQTVAGTWRYVLNARDRIAAVTPAD
                     VQRVAAQYLTKPNRTVGVLVKKAQDKKMAAAGEVAR"
BASE COUNT          925 a         1478 c         1256 g          797 t
ORIGIN      
        1 acaaagatcg ccagggcaat ccaacccacc agtacacgga ttccccctag ttcacgatgg
       61 ggattctgca ccggtggatg ggccagtccc atcatgccgg caagccccac ccacaagaac
      121 caacccttcc agcccaacca gccgaagacc atgaggatcg gcacgagggc gaccgccacg
      181 ctgcgttgcc gatctcctaa taacgcatag gccacatgtc ccccgtcgag ctgaccgatc
      241 ggaagcaggt tgagcgaggt gataaagagc ccgaaccagg cggcaaaccc aatcgggtga
      301 aggatcacat ccgcggtggg agcgagcggg ccgatcacca cccacgacgc aaactgaagg
      361 aggagcggct cgccgagatg catgccataa ctggtttgaa tcggcaccac cgtcgagagc
      421 cgaaggccca ccaccagcgc gatcaccgcc accacaaacc cggcgattgg ccccgccacg
      481 ccgatatcga acagggcccg gcgatccgtc aaaggtgccc gcatgcggat aatggcaccg
      541 aacgtgccga cgaaatgcgg caaccccggc acgaagagcg gcagcgatgt cggcacgcca
      601 tggatgcgcg acaacacata gtgaccgagc tcatgcgtca ccaggatacc cagcagggtg
      661 gcggcaaatg gaacaccccg ccataacgag ccgggatcat cgacgagaaa attccacggg
      721 ccgacgagtg gattggtgtt ggtttggtag gccccggccc agaggacggt aaagacggta
      781 agcaggaaca ggagcgccgg aagcatccat gcagagaact gcggacgttc ctcctcccat
      841 tcgtcctcgt cgctctcgtc ggacgaaagg gaaatcggtt cgtccggaga ccgcggctgc
      901 tccgacgagt cgtcacggtt catgcccggc ctccagtcct agcaggatgc ggaaaaagac
      961 cgccggcggc gttctcgcat cgcgaagcga ctcaccgtac tgcacgagta cgattcgccc
     1021 cttcgctcgc tgcggccttg ctggacggcc tttttgcgca tcctgcgggt gattctgata
     1081 ccgacacgac gcacgagttg atcataacgt cttgcgcaac aatcgagttg ttccgcaccc
     1141 tgctaggagt agaacgggtt gtggagtaac tcttcacgaa ccgtagtagc cggtccatgt
     1201 cccgggaaaa gccttgtgtc gggagccagc gtcaacacct gcttgcggac cgattccaga
     1261 tgggtctcat agagcccggc tggattggag cggccgatgg agccggcaaa cagcgtatcc
     1321 cccacaaaac agatcggggc accggcctgc tcaagacggt aacagacccc gcccggtgta
     1381 tggcccggtg tgggcatgaa ccggatcgtc agcccgccga ccggcagcgt ctcgcctccc
     1441 tgcggcacat gcaacacctg cggcgatggc cgccagctca agagatcgaa atcgtcaggg
     1501 ccaaggtaga ccggcaccgg gtatgccctg aggattgcct caatgccctc ggcatgatcg
     1561 gagtgaccgt gcgtcagaca gatggcccgc aaccgaagct tccgctgggc caacagcgcc
     1621 aacatcccgc tcgcattgta ggccgtatcg acgacgacgg cttctccgcg atcatgaacg
     1681 acgtagccct tgacctcata cccgccgatc gacccaaaca cggtctccac atgtggcacc
     1741 gaggaaggca aggcctcagg agtccaaccg tccaccacct gtgtcagcgg ttccgttctc
     1801 agtccaagcg cccgggcaac agcctggacc tgttcgcgac tccgtggcga ttgcccccgt
     1861 tccagctcac tcacttcgac ttcagacaag ccactcgcac gcgccacatc agccatcgac
     1921 cgctgctgac cgttgcgagc cttcttcaga atatcgctga gttcgtcttc aagaggcata
     1981 ggagtctccg gcctaagaac ccacgaacgc gtttaacttt ccgggaccag cgtaatttcc
     2041 ttcacctcac cgaaactggc cagcactttc gacagggcct caccggaccc gacggccaga
     2101 atccgcagcc gctcgggatt aaaatgggcc ttcgccgccc gctgaatatc ctctttcgtc
     2161 agcttcacca ccctgtcgcg gatctgctgc agccaatctt tcggcagccc gtcatattca
     2221 agatccatca aacgccccac aatgctcgac gcgctggtga aggaaaacac gaacgagttc
     2281 acataggctt ccttcgcttc ttccagttcg gtgtcggtca ccggctcatt gcgcatccgc
     2341 tccatgttgg ccacgaaacg attcaccact tcctgagtcg aagacagctt ggtttcggcg
     2401 cgcatcagcc agacgccttc atcgtacaca ctggcccgca ggccgctgcc gacggaatag
     2461 gccaagccgc gtttcgtgcg cacgtcgttg aagagccggc tgcggaacga actgccgccc
     2521 aggatgtcgt tggcgatcgc gaccgcgacg tagtcgggat cggtctcctt gatggtcaga
     2581 tgtcccaccc gcaggtgcgt ttgcgacgtg tccttgttca cgaaccgtac gagaccggtc
     2641 ttggcatcgg gctcggacac agcgggaatc gtgacagcgg gtacgttgcc cttggcccaa
     2701 tcgccgaagg cggcacgcaa cagggccagc atgtcggcct tctcgaaatc gccggtcacg
     2761 ccgaggatga tgccgttggg atgaacggtc cgctgatgaa aggccaccag atcttcacgg
     2821 gtaatcgcct cgatcgaacg aaccgacgtt tcacgggcgc tcggatgggt cgggccgtag
     2881 agcagcttgg caaactcccg cccgacgatc gagccgggac tgtcctgccg ccgcctgatg
     2941 ccttccaacg cctgcaattt cgctaactcc acccggccct gctcgaacgc cggccgacgc
     3001 aacagatcgg cgaagatctg cagcccgcgc ttcagatctt tcttcagcac atcgagagag
     3061 gccgagccgg attctttcgc aaatccgatg gacatcgaag cggccaattg ttcgagctct
     3121 tcatcgacct catcgggcga cattcccgcc gaaccgcccg tgcgcatcac cgccccggtc
     3181 ataccggcca ggccaacctt gtccgccggg tccagccagc tgccggttct cagcgtgaca
     3241 ttgatcgtca ccagcgggag ttcgtgatct tcgagcagat agaccaccat gccgttttcc
     3301 agcaccacac gctccgcatc cggcgggtta aattccacgg tcgggaatcg catcgtgcga
     3361 ggatcgttcg acgtcgggtc cgccgccaat cctaccgtcg cctgcaggac gatcatgaag
     3421 accatcgcca ggaccgacac ccatcctctg tctgttctca ttcgacgtcc tccgccatct
     3481 tcacgtttca cgagagacgc ttcacgaatc acgcaggtca tcgggccacc tcacccgcag
     3541 ccgccatttt cttgtcttgc gccttcttga ccaggacacc gaccgtccgg ttcggcttcg
     3601 tcaaatactg ggcagccacc cgctgcacat cggccggggt caccgccgcg atccggtcgc
     3661 gcgcgttcag cacataccgc caggtaccgg ccacagtctg ataaaaggcc aactgcgagg
     3721 cgaggccgct gttcgaacgc agggatcgca ccagatccgc atcgagtcca ttgagcaccc
     3781 gctcgaactc cttggtcgaa atcggctcgg ccttcaaccg gtcgagttcc tcgtaaatcg
     3841 ccgactccac ctccggcgtg gtatgaggag cgagcggagt cgccgcgatc acgaacaggt
     3901 tcggggcgcg gacgccggga taattcgtat cggacaacac cgaagccgcc agccgcttgt
     3961 cacgcacgag tgtgctgtag agccgcgacg tcacgccttc ggtcaacact tcgtcgatca
     4021 cgtcgaatac gaaatcgtcg ggatggccga tggtgggctt gtggtagccg atcgccaacg
     4081 cgggctcggc atcgaattcg atttcgacgc gtcgctcacc ccgttgcgga ggttcctccg
     4141 tcaccaggga tggaacggga ggcgcggccg gaattttccc gaaggtctgc tcgatcaacg
     4201 caatgacttc tttcggattg atgtccccga caatcgccac cgtcgcattg ttcggtccgt
     4261 aataggtctt gaagaacgcc tcggtcgccg ccggcgtgag ggacaggatg tccgatcccc
     4321 agccgatagt cggcacaccg tattgatgcg cctggaacgc cgtggaagtg aacgtttcgt
     4381 agagcaggcc gttcggactg tcgtcggtcc ggagccgccg ctcctccatg accacgcccc
     4441 gctccttata aaattc
//
LOCUS       DAOQRM010000191         4367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_21450_length_4367_cov_19.391002, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000191 DAOQRM010000000
VERSION     DAOQRM010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21450_length_4367_cov_19.391002"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /gene="trpS"
                     /locus_tag="PKK81_11880"
     CDS             complement(<1..881)
                     /gene="trpS"
                     /locus_tag="PKK81_11880"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNV33630.1"
                     /translation="MTTGQKRVLSGMQPSGLLHLGNWLGALENWNALQEQYECFFFVA
                     DWHALSSNYADTSRIKEYVRELLIDWLAAGIDPKRATVFVQSQVPDHAILHLLFSMII
                     PVSWLERNPTYKEKQEEIKERDLSTYGFLGYPVLQAADILLYKPDFVPVGKDQLPHLE
                     LTRELARRFNSLYRPVFPDPQEHLTKFAKVLGTDGRKMSKSYGNAINLSDQEPVVRQK
                     IKTMITDPARVRRHDPGNPDVCPVYDFHKIFSPLPVIEQVNQDCRKAAIGCIDCKKLV
                     ADRVVERLAPMWDARATL"
     gene            complement(1061..3055)
                     /locus_tag="PKK81_11885"
     CDS             complement(1061..3055)
                     /locus_tag="PKK81_11885"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNV33631.1"
                     /translation="MVPRSVPRAPHLRACSVALAVAFLAGLVTLGEAAPTKKNPAPSR
                     TEPSKPPTAAGQSVLDQAKRLIDSEQPEAAAVMLRRFIESGPPPDLLDDAYLLMAAAM
                     FGMKEHAETVRYVNQLLGEFPSSDLADRAKLLLAKTHARAGNLDLALPLLSEVRSLSA
                     DPAIKRDALRLTGEFQAQKKDYLRAIQAWLDEIPLDAGDQAHETEGQIRQLVNEQLDA
                     PALVRVREAYPKSFPGDLASIKLIELHTAAGEDHLVERDLRLFLSRFPNHPYAAKAAD
                     LQAVVRTKFKSHPYSIAAIFPMSGKLAPFGAEVLNGIQLALERSKDGGETPSIGLIVK
                     DTESDRAAFLDELSGVLSDDRPLAVIGPLLSKNLPVMAEMAERTRIPLITPSATAPNL
                     RRFGSYVFSTALTYGHQAKRVADYAIREQQYKRVAILYPDTAYGRDLARLFAQEVRQQ
                     DGELIVSEPYKEGDSDFRAVIGKLKAEDLKKYGVEVQVDNDPAKTGIRQGGKKGKRLL
                     YSPGFDAVFIPGRSLDVGLLAAQLAFFDIAVPLLGANGWNTPDFARVADRTVEGGVFA
                     DGFFAESSSPVVQEFVERYRKRFQATPSLFAAQGYDAARLAVEAIKRGATTGEGVRDF
                     LMMQHDLPTLSGPSGFSPDGTLNRRVFLIQVKQGKFVPLD"
     gene            3219..4169
                     /gene="xerD"
                     /locus_tag="PKK81_11890"
     CDS             3219..4169
                     /gene="xerD"
                     /locus_tag="PKK81_11890"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNV33632.1"
                     /translation="MSRESEDLSAPVPERLPLEPFIERYWDHVRIARGLSRNTLLAYQ
                     RDVATFQRYLRDRQVSDVREMSPPLLAGFLDHLHRSGLASSSRARALAAVRSLFRFLK
                     QEGMIAANPTVSLRSTSRARRLPKTLSPEEVTRLLDLPPRPLPEDHRDRAMVEVLYAA
                     GVRVSELISLRVDQCNLDVGYVGITGKGDKQRVVPIGRPAIEALQAYLLAARPALLKQ
                     RSSRFVFVSRRGTPLTRQAFWKLLRARAHRAGIARIPSPHMLRHSFATHLLQRGADLR
                     SVQAMLGHADIATTQIYTHVDSSQLKKVHTACFPRNRSRR"
BASE COUNT          771 a         1254 c         1416 g          926 t
ORIGIN      
        1 agggtggcgc gggcgtccca catcggtgcc aggcgctcca ccacccgatc ggccaccagc
       61 ttcttgcaat cgatgcaacc gatagccgcc ttgcgacagt cttgattgac ctgctcgatc
      121 accggcagcg gggagaaaat cttgtgaaag tcgtagacgg ggcagacatc cgggttgccg
      181 gggtcgtggc gtcgcacccg cgccggatcg gtgatcatgg ttttgatctt ctgccggacg
      241 acgggttcct gatcggagag gttgatggcg ttgccgtagc tcttgctcat cttgcgcccg
      301 tcggtgccca ggaccttcgc aaacttggtg aggtgttcct gcgggtctgg aaacaccgga
      361 cgataaaggc tgttgaaacg gcgcgcgagt tcgcgagtga gttcgaggtg cggcaactgg
      421 tctttgccga ccggcacgaa atcgggcttg tagaggagga tgtccgcggc ctgcaggacc
      481 gggtagccca gaaagccgta ggtgctcagg tcgcgctcct tgatctcttc ctgcttctct
      541 ttgtaggtcg ggttccgttc cagccaggag accggaatga tcatggaaaa caacagatgg
      601 agaatggcat ggtccggcac ctgggactgg acgaaaacgg tggcgcgctt cgggtcgatc
      661 ccggctgcca gccaatcgat cagcagttcc cgcacatatt ctttgatgcg gctggtgtcg
      721 gcgtagttgg aggataaggc atgccagtcc gccacgaaga agaagcactc gtactgctcc
      781 tgcagcgcgt tccagttctc cagcgcgccc aaccaattgc caagatggag caggccgctc
      841 ggctgcatcc cgctgagtac ccgtttctga cccgtcgtca tgccatcact cctgtgttct
      901 agcaggatgc tgaaagagcc cgccggcctc gtcctcgctt tgcctcggtg cttcgctcgc
      961 tgcggccttg ctggacggcc ctggtgagca tcctgagtcg ctgctcgatc tcgcctgaaa
     1021 tttcaatgga tcgattttct ttccaccggc accgcacccg tcagtccagc gggacgaact
     1081 ttccctgttt gacttgaatc agaaagacgc ggcgattgag tgtgccgtcg ggactgaacc
     1141 cgctcgggcc gctcaaggtc ggcaggtcat gttgcatcat taagaagtcg cggacgcctt
     1201 cgccggtggt cgccccccgc ttgatggctt ccacagccaa tcgtgcggcg tcatagccct
     1261 gggcagcgaa cagcgaaggg gtcgcttgaa accgtttgcg ataccgctcc acaaattcct
     1321 gtaccaccgg actgctgctt tcggcgaaaa atccgtcggc aaagacgcct ccctcgacgg
     1381 ttcgatcggc gacccgcgcg aaatccggag tgttccatcc gttggcccca agcaacggaa
     1441 ccgcgatgtc aaaaaacgcc aattgggctg ccagcagtcc cacatccagc gaacgtccgg
     1501 ggatgaacac ggcgtcgaac cccggtgagt agagcaggcg cttgcctttc ttgcctccct
     1561 gtctgatccc ggttttggcc ggatcgttgt cgacttgcac ttccacgccg tatttcttca
     1621 gatcctcggc tttgagcttg ccgatcacgg cgcgaaagtc gctgtcgcct tccttgtagg
     1681 gctcgctcac gatcagttct ccgtcctgtt gccggacttc ttgcgcgaac aagcgggcca
     1741 ggtcgcggcc gtaggcggta tcgggataga gaatggcgac gcgtttgtac tgctgttccc
     1801 ggatcgcata gtccgccacg cgtttggcct ggtggccata ggtaagcgct gtgctgaaga
     1861 cgtagctgcc gaatcgtcgc aggttcgggg cggtggcgct gggcgtgatc agcggaatgc
     1921 gtgtccgttc ggccatttca gccatcaccg gcaggttctt ggacaggagc gggccgatca
     1981 cggcgagtgg gcgatcgtcg gacaatacgc cggagagttc atccagaaat gcggcacggt
     2041 cagattccgt atccttgacg atcaggccga tcgagggtgt ctcgccgccg tctttggagc
     2101 gctcgagggc cagctggatg ccgttcaata cctcggctcc gaaaggggcc agctttccgg
     2161 acatgggaaa gatggcggcg atggagtagg gatgtgattt gaactttgtc cgcaccaccg
     2221 cctggaggtc ggccgctttt gcggcatagg gatggttggg gaagcggctc aggaagaggc
     2281 gcaaatcccg ttcgaccaga tgatcttcgc cggcggcggt atggagctcg atgagcttga
     2341 tggaggccag atctcccgga aatgatttcg ggtaggcctc gcggacgcgc acgagcgccg
     2401 gagcgtcgag ttgctcattg acgagttgcc ggatctggcc ttcggtttca tgggcctggt
     2461 cgccggcatc cagcggaatc tcatccagcc aggcttgaat ggcccggagg tagtctttct
     2521 tctgcgcctg gaattcgccc gtgagccgca gcgcatcgcg cttgatggcg ggatcggcgg
     2581 agaggctgcg gacctcggag agcaagggga gcgccaggtc gagattgcct gcgcgcgcgt
     2641 gagttttggc gagcagcagt ttggcccgat cggccaggtc agagctggga aattcgccga
     2701 gcagctgatt gacgtagcgg acggtttccg catgttcctt catgccgaac atggccgcgg
     2761 ccatcaacaa gtaggcgtca tcgaggaggt cgggaggggg accgctttcg atgaagcgtc
     2821 gcagcatgac cgctgcggct tccggttgct cggagtcgat caggcgcttg gcttggtcga
     2881 gcacggactg gccggcggca gtcggtggtt tcgatggctc tgtgcgggac ggcgctggat
     2941 tctttttggt cggggctgcc tcaccgagcg tgacgagacc cgcgaggaac gccaccgcga
     3001 gggcgacgct gcacgcgcgg agatgcgggg cgcggggtac tgatcgagga accattgact
     3061 cctgtgcatg gcgaacgctc tcgccgcaag tgaggctact ataggaaaca ggtgcggggg
     3121 tgtcaaggta actcgaggtc gatgcgttgt gttcggaagg aggcttggct atagtcgatg
     3181 cagcctgttc ctcgtcgcat tcagcaaggg tgtttcccat gagccgtgag tcggaggatc
     3241 tgtctgcgcc ggtcccggag cgactcccgc tggagccgtt catcgagcgc tactgggatc
     3301 atgtgcggat cgcgcggggc ttgtcacgca atacgctgct ggcatatcag cgggacgtcg
     3361 cgacgtttca gcgctatctc cgggatcggc aggtctctga tgtgcgcgag atgtcgcctc
     3421 cgctgctggc cggatttctc gatcatctgc atcggtcagg cctcgcgtcg tcgtcccgcg
     3481 cgcgcgcgct tgccgcggtc aggagtctgt tccgctttct gaagcaggag gggatgattg
     3541 ccgccaaccc gaccgtgagt ttgcgcagca cctcgcgcgc ccgacggttg ccgaaaacgc
     3601 tcagtcccga ggaggtgacg cgtcttctgg atctgccgcc ccgtccattg ccggaagatc
     3661 atcgtgatcg cgcgatggtg gaggtgctct atgccgccgg ggtgcgtgtg tccgaattga
     3721 tctcgctgcg ggttgaccag tgcaatctgg atgtcggcta tgtcggtatc accggcaagg
     3781 gcgataaaca gcgtgtcgtg ccgatcggac gtccggcgat tgaggcattg caggcctatc
     3841 tcctcgctgc gcgaccggcg ctgctgaaac agcggtcgtc acgatttgtg ttcgtgagcc
     3901 gtcgcggcac gccgctcacg aggcaggctt tctggaaatt gctccgcgcg cgtgcgcacc
     3961 gggccggcat cgccaggatt ccttcgcctc atatgctgcg acattccttc gccacgcact
     4021 tgttgcaacg gggggccgac ttgcgatccg tgcaggcgat gttggggcat gccgacatcg
     4081 cgacgacgca aatttacacg catgtggatt cgtcacagtt gaaaaaagtc cacactgcgt
     4141 gtttcccgcg caacagatcg cgtcgttgag cccccttcgt gccgtccgtg agtaccaccg
     4201 tgatagaaat ttttgctttc cacgaatcct gcaagaataa tgcatgattc ctggttgaca
     4261 ctgctttgtg gagttgatag catcccgaaa atctgccggg aacgagaaaa atcgggcgga
     4321 tagctcaatt gggagagcgc tgcccttaca aggcagaggt cacaggt
//
LOCUS       DAOQRM010000192         4332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_21734_length_4332_cov_21.169044, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000192 DAOQRM010000000
VERSION     DAOQRM010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21734_length_4332_cov_21.169044"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="PKK81_11895"
     CDS             complement(<1..1406)
                     /locus_tag="PKK81_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV33633.1"
                     /translation="MSETPSPANPVPTQDNAHPILTHERSLANKIAKGSEAGDILDQQ
                     LAMLGIQLVTQLNVLIKTSRIHGRTNAALDKPVDSMLTLVKTLAADHPVVLRLQNDFL
                     FLGDSHLKMSSQQMAVFASIIEALNKWHIGGVSFASTADSKDFREFAYLFVSLDPDKH
                     SLTDLQEAMQAAGIKGIELEDPRSLQLQHLNDAELAGGNTGARTSGSSNGSEGSKEKR
                     KALAKGGYLKASTALGDLVKNTRGGGTVSFKQAKRAIQNIVELMMQDESTLLGLTNLR
                     CHDQYTHNHSVNVSLLAMALGNRAGYPKVELADLGLSALFHDVGKCAISLDVLNKPGE
                     FTQEEWAVMRSHPIEGVFTLVKSRGINNVPARMAAASFEHHMNYDYSGYPKLKVSWTQ
                     TVASRIITIADCYDAMTSSRVYRREPMSPANVLKFMFGKSGQSFDPILLKLFVNCVGI
                     IPIGSLVRLDSGPLAVVI"
     gene            complement(1424..3280)
                     /locus_tag="PKK81_11900"
     CDS             complement(1424..3280)
                     /locus_tag="PKK81_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV33634.1"
                     /translation="MEPTAPLEEGAPLVPFVKAGVDPEVAAIKQLLKLLDKAAKSART
                     YGTKNPVAQRFFQQFYDDLTSHLATHSRLPLLVQRNQLFFKDEVVYQPERDATGDSFA
                     FKMYSDGIRELSFHQGLTQEDLAYFLDALWGTPTDEAGSVEQPADVEEDDDIVTRLWA
                     RNLDTITLVTAEELVRSSGFGPDELELQTQGYMSMSVTSLRELLDRERAAAAPEPAGQ
                     PGTADQPGGGTSGGGTSRNRRFQANVVGYDVSQEEHDALTQEIQRETERDATAYILDI
                     LTAVLASEPSPALLSKLFDVWDSVLDVLIRGGHWTQIETVLTLLQDADAVRPDLSETH
                     KQQLAGLFEGLSRPERLKAIGLHLNHALHAKTEGLLTLLLMMKQDSIPGLCSLLANLD
                     APSHQAVVMEALQTLARDHSDAIVRGLTDKRPTYVRNLLTLISRWNDIRFADCIEKTL
                     RHPESTVRREALRILATLRPSGNGTKLVGLLSDSDETVRLTAMKVLSSGQYSAGFTVW
                     APFVSADDFHDRSPAEKRAVYLALKQTAADEAVPYWQGLLTEWSWTNRKKKEELALLA
                     ADILGKLATPAAVAALEIGQKKGGAAVRQACTSALSVANRQHRQSIPSAANS"
     gene            3432..4103
                     /locus_tag="PKK81_11905"
     CDS             3432..4103
                     /locus_tag="PKK81_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNV33635.1"
                     /translation="MTASTQLPLIVCFGDSLTAGYQTPGPANAHEQETPYGHVLQECL
                     GHRGRVEISGICGEVTGEMVLRFRGTVLDRKPQMVIILGGTNDLGWNADPAEIMRNLV
                     KMYESARAASIVPVPVTVPSIRVDAGADHPDAAAWLAGHIQRRQQLNRLIADYAGRKG
                     LSYFDLFTATADPDSLMLAEPYSNDGLHLTTSGYRLFGRLLYEQLFAPTAAGSLLTPS
                     RPSGP"
     gene            4100..>4332
                     /locus_tag="PKK81_11910"
     CDS             4100..>4332
                     /locus_tag="PKK81_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNV33636.1"
                     /translation="MIRDVTDRDFPREVEQASTPVLVEFWQPGCGHCLALLKQLEQLQ
                     EEVAPVVKIVKMNVQENFQIPAELDISSLPALAL"
BASE COUNT          774 a         1244 c         1366 g          948 t
ORIGIN      
        1 ttgatgacga cggccagagg gccggagtcg agtcggacga gactgccgat cgggatgatc
       61 cccacgcagt tgacgaagag cttgagcagg atcggatcga acgactggcc gctctttccg
      121 aacataaact tcaacacgtt cgcgggggac atgggttccc gtcggtacac ccgggaggat
      181 gtcatcgcgt cgtaacaatc ggcgatcgtg atgatccggc tggcgaccgt ttgagtccag
      241 gaaaccttga gtttggggta tcctgagtag tcgtagttca tatggtgctc gaacgaggcg
      301 gccgccatcc gcgccggcac attgttgatg ccgcgggatt tcacgagggt gaacacccct
      361 tcaatcgggt gcgaccgcat cacggcccat tcttcctgtg tgaattctcc gggtttgttc
      421 aacacgtcca gggagatggc gcatttccct acgtcgtgga acagcgccga cagcccgagg
      481 tcagccagct cgactttcgg ataaccggcc cggttgccca gggccatggc gagcagtgag
      541 acgttgacgg agtgattgtg cgtgtattga tcgtggcagc gcagattggt cagtccgagg
      601 agggtcgatt catcctgcat catcagctcg acgatgttct gaatcgcgcg tttggcctgt
      661 ttgaagctga cggtcccgcc gccgcgcgta ttcttgacca agtcgccgag cgcagtcgat
      721 gctttcagat atccgccttt ggccagggcc ttccgtttct ctttcgaccc ctccgagccg
      781 ttcgatgagc cacttgtacg tgccccggta ttgcctccag ccaactccgc gtcgttcaga
      841 tgctgaagtt gcagcgagcg gggatcttcc agttcgatgc ccttgatgcc cgcggcctgc
      901 atggcctcct ggagatcggt aagggaatgc ttgtccggat ccaggctgac gaagagataa
      961 gcaaactccc gaaaatcctt tgagtcggcc gtcgacgcga acgacacccc gccgatgtgc
     1021 cacttgttca aggcttcgat aatgctcgca aacacggcca tctgttgcga gctcattttc
     1081 aggtgactgt ctcccagaaa caaaaaatcg ttttgcagcc ggaggacgac cggatgatct
     1141 gccgcgaggg ttttcaccag cgtgagcatg gagtccacgg gtttgtcgag cgcggcattc
     1201 gttcgtccat ggatccgcga tgtcttgatg aggacgttca actgggtgac gagctgaatc
     1261 cccagcatgg ccagttgctg gtcgaggatg tccccggctt ctgagccttt ggcgatctta
     1321 ttggccaggg accgctcatg agtgagaatc ggatgggcgt tgtcctgagt cgggaccgga
     1381 ttggcagggg aaggcgtctc gctcacaggt cgtctcctcg caatcaggaa ttggctgccg
     1441 atgggatgga ctgccggtgt tgtcgattgg cgacggagag cgcggaggtg caggcttggc
     1501 ggacggctgc gccgcccttt ttctggccga tttcgagcgc cgcaacggct gcgggtgtcg
     1561 ccagcttgcc gagaatatcc gccgccagca aggccagttc ttctttcttc ttccggttgg
     1621 tccaggacca ctccgtcaac agtccctgcc aatagggcac ggcttcatcg gcggcagtct
     1681 gtttgagggc caggtagacc gcccgctttt cggccggtga tcggtcatga aagtcatcgg
     1741 cggagacgaa cggggcccag acggtgaaac cggcactgta ctgtccgctg gaaagcacct
     1801 tcatggccgt gaggcggacg gtttcatcac tgtcgctcag caggccgact aacttggttc
     1861 cgttgccgga ggggcggagc gtggccagga tccggagcgc ctcacgccgc acggtggatt
     1921 ccgggtgccg gagcgttttc tcgatgcagt cggcaaagcg gatgtcgttc catctggaga
     1981 tgagcgtcag cagatttctc acgtaggtcg gccgtttgtc ggtcaagccg cgcacgatcg
     2041 catcggaatg gtctcgcgcg agcgtctgca gcgcttccat gacgacggct tgatgcgaag
     2101 gcgcatctag gttggcgagc aacgagcaga gtcccgggat cgaatcctgt ttcatcatca
     2161 ggaggagggt cagcaggcct tctgtcttgg cgtggagggc gtgattcagg tgaagtccga
     2221 tcgccttcag ccgttcgggt cggctcaatc cctcgaacag gccggcgagc tgttgcttgt
     2281 gagtctccga cagatcgggg cgcaccgcat cggcatcctg caacagggtc aatacggttt
     2341 cgatctgtgt ccagtgtccg ccgcgaatca gcacatccag cacgctatcc cagacgtcga
     2401 acagtttgct gagcaaggcg ggggagggtt cggacgccag cacggctgtc aggatgtcga
     2461 ggatgtaagc cgtggcgtca cgctcggttt cacgctggat ttcctgcgtg agcgcgtcat
     2521 gctcttcctg agagacgtca tacccgacga cgttggcctg gaatcgccga ttcctgcttg
     2581 tgccgccgcc gctggttccc ccgcccggtt gatcggctgt tcccggttgt cccgctggct
     2641 cgggggccgc tgccgcgcgc tcacggtcga gtagttcgcg cagcgaggtg accgacatgc
     2701 tcatatagcc ctgtgtctgc agttcgagct cgtccggtcc gaaaccggac gaccgaacga
     2761 gttcctctgc ggtgaccagg gtgatggtgt ccagattcct ggcccacagc cgcgtcacga
     2821 tatcgtcgtc ttcttccacg tcggccggct gctctactga tcctgcctcg tcagtgggcg
     2881 tcccccagag cgcgtcgagg aaataagcca gatcctcctg cgtgaggcct tgatggaagc
     2941 tcaattcgcg gattccgtcc gagtacattt tgaacgcgaa gctatcgccg gtcgcatcgc
     3001 gctccggttg atagacgact tcgtctttga agaacagttg attgcgttgt actaggagag
     3061 ggagtcggct gtgggttgcg agatggctcg tcaggtcgtc gtaaaactgc tgaaagaatc
     3121 gctgtgcgac ggggttcttt gtgccgtagg tccgtgccga tttggccgct ttgtcgagca
     3181 gcttgagcag ctgcttgatc gccgctacct ccggatcgac cccggccttc acaaagggga
     3241 cgagcggagc gccttcttcg agcggtgctg tcggttccat atgtagtcag tcgtaccgtg
     3301 aggtctcttt ttcgtcaaga aaatagccgg tccgcgcagg tgttccggaa acctgccggg
     3361 cgtcgagacg cgccggtcga tcgccggacg cacgcattgc gtcgcactgt ccgggacagt
     3421 agaatcgctt cgtgactgca tcaacccaac tgccgctcat tgtttgtttc ggtgatagcc
     3481 tcacggcagg gtatcaaacc cccggcccgg ccaacgctca tgagcaggag actccctatg
     3541 gtcatgtctt gcaggaatgc ctcggccacc gggggcgggt cgagatcagc gggatctgcg
     3601 gcgaagtcac cggcgaaatg gtgctgcggt ttcgcggcac ggtgctcgac cgcaagcctc
     3661 agatggtcat cattctcgga ggaacgaatg atttgggctg gaacgccgat ccggcagaaa
     3721 tcatgcggaa tctcgtgaag atgtatgaat cggcgagggc cgcgtcgatc gttccggttc
     3781 ctgtgaccgt gccgtcgatt cgtgtcgatg cgggagcgga ccatcccgat gccgccgcct
     3841 ggctcgccgg gcatattcaa cgacggcaac agttgaatag gttgatcgcg gactatgccg
     3901 gtcgaaaggg cttgtcctat ttcgatctgt tcaccgcgac ggccgatccg gactcgctca
     3961 tgctagccga accctactcg aacgatggcc tccatctcac gaccagcggc tatcggctgt
     4021 tcggccggtt gctctacgag cagctctttg ctcctacggc ggccggctct ctcctcacac
     4081 cttccaggcc gtctggccca tgatccggga tgtcacggac cgcgacttcc ctcgtgaagt
     4141 ggagcaggcg tcgacgccgg tgctggtgga attctggcaa ccgggctgcg gtcattgcct
     4201 ggccctgttg aagcagttgg agcaattgca ggaagaggtc gcgccggtgg tgaagatcgt
     4261 gaagatgaat gtgcaggaga attttcagat tcccgcggaa ctggacatca gctcactgcc
     4321 ggcactcgcg ct
//
LOCUS       DAOQRM010000193         4332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_21736_length_4332_cov_12.605097, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000193 DAOQRM010000000
VERSION     DAOQRM010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21736_length_4332_cov_12.605097"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKK81_11915"
     CDS             complement(<1..743)
                     /locus_tag="PKK81_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNV33637.1"
                     /translation="MNRRILLALGILGTVGLSLLPFLWFVLTSFKSQGEIEVAPPAWW
                     PSGNLGFYRSALFDHHLFDYVLNSVVVAGATTVLALLFAIPAAYALARLTIPGKQGIL
                     AVLLCVSMFPQMAIAGPVWRLLDTIGGLNHRWGVVLPYVALTLPLAIWILASFFKELP
                     PELEDAARVDGCGPWTTLFRITLPLATPGIFTAAILILIYAWNEFFFALLILTQPEQQ
                     TLPVGIALFQGEFTMPWGELAAASVVATLP"
     gene            complement(740..1651)
                     /locus_tag="PKK81_11920"
     CDS             complement(740..1651)
                     /locus_tag="PKK81_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNV33638.1"
                     /translation="MIRPSPILRHRDSLAAWTMVAPALLVTMVFALYPVLDSLWLSLH
                     NIFIGLPQLGSPFVGLDNYVALLRDPVAQQALVVTLAFVGLSTILELACGLIIALVIH
                     ERFRGRGLVRAAILIPWAIPTVVASQLWRYIFNDQYGFANLLLFGERVTDYVPWLAYP
                     GVAFGIVVLADVWKTSSFAALLILAGLQVIPDDLYDAARVDGATVWQRFGHITLPLLK
                     PALLLALLFRTMDAFRVFDLVFVMTQGGPGDATQVLQFYGYQTLFTEGRIGYGSAVSV
                     VVFLMILALSLTYLRAIGSSLLERRRT"
     gene            complement(1648..3042)
                     /locus_tag="PKK81_11925"
     CDS             complement(1648..3042)
                     /locus_tag="PKK81_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV33639.1"
                     /translation="MRRIVRAFFTCSLLQSGPRHPLRANHQRRAALLLLWLVLFGPPL
                     NDSIAGGPAAPSKGQTTPITLRFVSWKPDHPRVWDDALDEFTKAHPHISIVRELAPHS
                     STAYHDLLTQKLKNRDATVDVFFMDVIWVPEFAEAGWARRLDERFTPAMREEFLPATI
                     EVGRYSDHLYGVPSRIDAGLLYYRSDLLAKYGFSPPTTWDELARQADAIVAGERRNNP
                     TLRGYTAQFKQYEGLVCNLLEFIDGHGGSLLTSDGTHSTLGRPETLAAVQFVRDRVIG
                     RLASRAALTYQEPESLSVFLQGHAVFHRNWPYAWELANNRTRSTIAGQVAVMPLPGFT
                     PGRTAAALGGWLYGISAYSQHPDEAWALIEFLSSQAMQKKFTQEAGIAPSRQALFSDP
                     DLLAAAPQLRNHFAVLRSATARPRSPLYPAISHLLQRYFSRALAIDGLDLAQEAAVTD
                     AHIDRLLALTRIAP"
     gene            3662..4330
                     /locus_tag="PKK81_11930"
     CDS             3662..4330
                     /locus_tag="PKK81_11930"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNV33640.1"
                     /translation="MKRGLRQWFSSPVGVLPRMLVTLAVMLIGSVMMLSEAHALQASP
                     TSLSFQAVQGGPNPSSKTVKVYKSSSRKVSWSGKDNAGWLSLSPTSGTLGSSTQVTVS
                     VNISGLAAGTYSGTVTITTSRGSTVAIPVTLKLSAATSSSTTGTTAALAWNANAESDL
                     AGYKVYSGTSSGQYGASVDVGKATSYQFSNLKLGTTYYFAVTAYDVNGNESLRSSEVS
                     KSIY"
BASE COUNT          934 a         1213 c         1416 g          769 t
ORIGIN      
        1 ggaagggtcg ccaccacgga ggctgcggcc agctctcccc agggcatggt gaattctccc
       61 tggaagaggg cgatgccgac aggaagggtc tgttgctccg gctgggtcag gatcaggagg
      121 gcgaagaaaa actcgttcca ggcatagatc aagatcagaa tcgcggcggt gaaaatcccc
      181 ggggttgcca gcggaagcgt gatccggaac agagttgtcc agggcccgca accatccact
      241 cgtgccgcat cttccaactc cggcggcaat tccttaaaga agctggccag aatccagatc
      301 gccaacggca gggtgagggc cacatagggc agcacgacgc cccaccggtg gttgagcccg
      361 ccgattgtgt cgagcagtcg ccagacgggt ccggcaatgg ccatttgagg aaacatcgag
      421 acgcagagca acacggccag aataccttgt ttgccgggga tggtcagccg ggctaatgca
      481 taagctgccg ggatggcgaa caggagagcc agcacagttg tggcgccggc cacgacgacg
      541 ctgttcagca catagtcgaa gagatgatgg tcgaagaggg ctgagcgata gaatcccagg
      601 ttgcctgagg gccaccaggc gggcggcgcg acttcgatct caccttgcga cttgaacgac
      661 gtgaggacaa accagaggaa cggcagcaga ctgagtccca ccgtaccgag gatgccgagt
      721 gcgagaagaa tccgacgatt catgttcgcc tgcgctccaa gagactcgag ccgatggcgc
      781 gcaggtacgt caacgacagc gccaggatca tcaggaacac gaccacggag acggcagagc
      841 cgtatccgat gcggccttcc gtgaagaggg tttggtagcc atagaactgc aggacctgcg
      901 tggcgtcgcc cgggccgcct tgggtcatca cgaacaccag atcgaagaca cgaaacgcat
      961 ccatggtccg aaataagagc gcgagcaaca gtgccggttt cagcagcggc agggtgatgt
     1021 gcccaaaccg ctgccagacg gtggccccat ccacacgcgc tgcgtcatag agatcgtcgg
     1081 gaatgacctg gaggccggcc aggatcagca gggcggcgaa ggaggaggtt ttccagacgt
     1141 cggccagcac cacgatgccg aaggccacgc ccggataggc cagccagggg acgtagtcgg
     1201 tgacgcgttc gccgaacaac aataagtttg cgaagccata ctggtcgttg aagatatagc
     1261 gccacagttg cgacgcgacg actgtgggaa tggcccaggg aatcaagata gcggcccgca
     1321 cgagaccgcg cccgcgaaat cgctcatgaa tcaccagcgc gatgatcagc ccgcaggcca
     1381 attcgagtat cgtggagagg cccacaaagg caagggtgac gactagtgcc tgttgcgcca
     1441 ccggatcacg aagcagcgcc acataattgt ccagcccgac gaacggactg ccgagctgcg
     1501 gcagcccgat gaaaatattg tgcaggctga gccagagcga atccaacacg gggtacagtg
     1561 cgaagaccat tgttacgagc agcgccggtg ccaccatcgt ccaggccgcc aggctgtcgc
     1621 gatgtcgcag gatcggtgag gggcggatca tggcgcaatc ctcgtgaggg cgagtagccg
     1681 atcgatgtgg gcgtcggtca cggccgcttc ctgcgcgagg tcgaggccgt cgatggcgag
     1741 ggcgcggctg aaataccgtt gcagtagatg cgagatggcg gggtacaggg gggaacgcgg
     1801 gcgagcggtt gcggaccgga ggacggcgaa gtggttacgg agttgcggcg ctgctgccag
     1861 caggtcggga tcggaaaaca gcgcctgacg ggagggcgcg atgccggcct cctgggtgaa
     1921 tttcttttgc atggcctggc tggagagaaa ctcgatgagc gcccaggcct cgtctggatg
     1981 ctgcgagtag gcgctgatgc cgtagagcca gccgccgagt gctgcggccg tgcgccccgg
     2041 agtgaacccg ggaaggggca tcaccgcgac ctgtccggcg atggtcgatc gagtccggtt
     2101 gttggccagt tcccaggcat agggccagtt gcggtgaaag actgcatggc cttgaagaaa
     2161 gacggagagg gattccggtt cttgataggt caacgccgcg cgtgaggcaa gccggccgat
     2221 cacccggtca cgaacgaact gcacggcggc cagcgtttcg ggtctgccca gagtggaatg
     2281 tgttccatcc gacgtcagca ggctcccgcc gtggccgtcg atgaactcca gcaggttaca
     2341 gacgaggcct tcatactgtt tgaattgtgc tgtgtagccg cgcagggtcg gattgttccg
     2401 tcgttccccg gcgacgatgg cgtcagcttg tcgggcgagc tcgtcccagg tcgtcggtgg
     2461 tgagaagccg tacttggcca gcaaatccga gcggtagtag agcagcccgg catcgatgcg
     2521 actcgggacg ccgtagagat ggtcggagta tcgacccacc tcgatggtgg cggggagaaa
     2581 ctcctcacgc atggctggag taaatcgctc gtcgagccgt cgggcccacc cagcttcggc
     2641 aaactccggc acccagatga catccatgaa aaagacatcg accgtggcat cacgattctt
     2701 cagcttctgc gtcaggagat cgtgataggc cgtcgagctg tgcggggcca gttcgcgcac
     2761 gatcgaaata tgaggatgag ctttcgtgaa ttcgtcgagg gcgtcgtccc acacgcgcgg
     2821 gtgatcgggc ttccaggaga cgaaacgcag cgtgatgggg gtcgtctgtc ccttggatgg
     2881 cgctgcaggt ccgccggcga tcgaatcgtt gagcggtgga ccgaagagaa ccaaccagag
     2941 caggagtagg gccgcacgac gttggtgatt ggcacgcaac ggatggcgtg gtccggactg
     3001 aagaagtgag catgtgaaaa aggcgcgcac aatgcgacgc atgacagctc tagcttatac
     3061 acagtccatc cggtctgtgc aaaggcacag ggcctcagct gcagtggaga tgcggcggcc
     3121 tgcctcttac gggcagtgtc tgcaggtcgc attgccctca gatccgaacc gtagacacgg
     3181 cacgggtcgg gcccgtcctg acgcgagacc cttgaacctt tgcgccggca ccgccttgtc
     3241 gacggctcgg cacttgcgga tctcatcatt ttttctcgcg cgacggcgag cacaagcgtc
     3301 tctccagtgc aagtcggctt ctcgtggaac atgtggtcga cggaatgtac caagttcctt
     3361 gagcggcaac acatcgatag gagtcaccac cgcttcctct ttctccgcac cggagacgag
     3421 gggctgcgtg cacattctct acccgtcctg aagtttcaac catacaccgg ccgcccaagc
     3481 cgcggcggag atacacgtaa atcctcacga gatagcacga ttttctcacg cacttcagct
     3541 tggcatgatc actgctctta gagtgtctgg ctggggcagt ggttcgtgtc acgagacgcg
     3601 cggtgagccg ctttgcgatt gtcgaagatg gtattcccta atgtcgcacg tgggaggtca
     3661 tatgaaacga ggtctgcgcc agtggttctc ttcgcctgtg ggtgtcctac ctcggatgct
     3721 ggtgaccctt gccgtgatgc tgatcggaag cgtgatgatg ctttccgaag cccatgcctt
     3781 acaagccagt ccgaccagtt tgagctttca ggccgtgcag ggtggtccca atccatccag
     3841 taagacggtg aaggtctaca agagcagcag tcgaaaggtc agttggagcg ggaaggacaa
     3901 tgccggttgg ctgagtctct ctcctacgtc cggcacgctg ggaagttcca cgcaggtgac
     3961 ggtgtccgtc aatatcagcg ggctggcggc cggaacctat tccggaacgg tgacgatcac
     4021 gaccagcagg ggaagcaccg tggcgattcc cgtgacactt aagctctcag ctgcgacttc
     4081 ttcaagcacc accggcacga cggctgctct ggcgtggaat gccaatgccg aaagcgactt
     4141 agccggatac aaagtctatt ccggaacctc ttccggccag tatggggcat cggttgacgt
     4201 ggggaaggcc acgtcctatc agttttcgaa cctgaaactc ggaacgacct actactttgc
     4261 cgtcacggcg tatgatgtga acgggaatga gagcctccgt tcgagtgaag tgagcaagag
     4321 catctattga cg
//
LOCUS       DAOQRM010000194         4315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_21862_length_4315_cov_13.723709, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000194 DAOQRM010000000
VERSION     DAOQRM010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21862_length_4315_cov_13.723709"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..544
                     /gene="rplU"
                     /locus_tag="PKK81_11935"
     CDS             230..544
                     /gene="rplU"
                     /locus_tag="PKK81_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNV33641.1"
                     /translation="MYAIIETGGKQYRVETGTVLQVESLPGDVGHSVQIDKVKLLHGE
                     GGLVVGQPVVAGATVTAEIIRQGRTRSITVFKKHRRKNYRRTRGHRQGFTQLRVTGIE
                     TK"
     gene            566..829
                     /gene="rpmA"
                     /locus_tag="PKK81_11940"
     CDS             566..829
                     /gene="rpmA"
                     /locus_tag="PKK81_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNV33642.1"
                     /translation="MATNKGGGSTRNGRDSNPQYLGVKAYGGETIKAGSIIVRQRGTK
                     FFPGLNVGLGRDHTLYAYVSGVVKFEGGRGRQKVSVYPVTAKA"
     gene            955..1986
                     /gene="obgE"
                     /locus_tag="PKK81_11945"
     CDS             955..1986
                     /gene="obgE"
                     /locus_tag="PKK81_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNV33643.1"
                     /translation="MSTFVDQVQILVKAGHGGNGACSFRREKFVPRGGPDGGDGGDGG
                     SVVVEATTRLSTLLDLRYQKHYEAEKGETGGGSNCHGRRGADVRIPVPVGTMVFDENT
                     QELLADLTKDGETCVIAKGGQGGRGNTQFATSTNRVPTQFEPGTPGEERLLRLDLKLL
                     ADVGLVGYPNAGKSTLIAAVSAARPKIADYPFTTLTPNLGVVRWTGEQTFVIADIPGL
                     IEGAHEGKGLGFQFLRHIERTSLLLHVIDISEWATEDPVASLEIMRHELTAYDDTLAA
                     RPFAVVGTKLDVKGEGERLERLRKYCQRRKIPFFAISAATREGLDECIRYIGQQVELL
                     RKTPCETNS"
     gene            1968..3089
                     /gene="proB"
                     /locus_tag="PKK81_11950"
     CDS             1968..3089
                     /gene="proB"
                     /locus_tag="PKK81_11950"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNV33644.1"
                     /translation="MRDELLRQAKRVVIKIGSSLIASRETGLSAERIERLAGEIAEVR
                     AQGREVLVVSSGAVVSGIKKLGLREYPKSLPLKQAAAAVGQSLLMWAYEKAFERLTLR
                     VAQVLLTHEDLADRRRFLNARHTLTTLIKFGVIPIINENDTVAVEEIRVGDNDTLAAQ
                     VAHLVDADLLIILSDVDGLFTVDPRKDPTAALIPLIPEITEDIEQRAGMSSTFEGTGG
                     MATKVRAAKKVGEYGVATLILNGTQTRLLPNVLSGQPGGSLFLARERRMNSRKHWIAF
                     TLRPRGHVQLDQGAVEALVRNGKSLLASGILDITGHFDAGDPVTCAGPDGKECAKGLV
                     NFSSEILTRIKGLKTADIQKIPGLQEYEEVIHRDNLVIL"
     gene            3192..3872
                     /gene="nadD"
                     /locus_tag="PKK81_11955"
     CDS             3192..3872
                     /gene="nadD"
                     /locus_tag="PKK81_11955"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNV33645.1"
                     /translation="MRLGLLGGSFNPIHRCHLSIARSARQLLQLDRVLFIPTGDPPHK
                     QPGTLAAASHRHRMVQLAIQDTPEFALTDIEIQRSGKSYSIDTVRAIQQEYGPDTVLF
                     FIIGLDAFLDLPSWKEADTLLKSCHFVVISRPSTSFQAMAAIPLFRDVPQTTLTALDE
                     VRQERADVAITNGQALTFLRLPPCDVSASEIRARLRSGASLANLLPTPVESYILREGL
                     YREDSERL"
     gene            3859..4281
                     /gene="rsfS"
                     /locus_tag="PKK81_11960"
     CDS             3859..4281
                     /gene="rsfS"
                     /locus_tag="PKK81_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNV33646.1"
                     /translation="MSVSESKAKALAVASAILDKKATDVLILHIATLTSVADYLVIGS
                     GESERQARAMADHVSDVLTAQGHRPLSTEGASSAKWVVMDFGDVIVHIFQKDIREHYA
                     LERLWGDAGQVRLPEERAVKAAPPTRTATRPARTRKRV"
BASE COUNT          944 a         1363 c         1226 g          782 t
ORIGIN      
        1 ttttcgctgg tttgtaacat gcgcgggata ataccagcgc ctcgcgacga ccgtcaagac
       61 aaccgcacgt agacatttcg atgcagagtc ttcaggaatt ccgccgctcc cgccctcctt
      121 cacgcagcgg tggctgaagc cagcttcgtt caatgacgca ctaattcttg acaccttctc
      181 cgccgctatg ataaacaccc acgttctgct gacgaggaga aagacgcaca tgtacgctat
      241 tatcgagaca ggcggaaaac agtatcgagt ggaaaccgga accgtactcc aggtggagtc
      301 acttccgggt gatgtcgggc actctgtgca gatcgacaag gtcaagctgc tgcatggcga
      361 gggcggactg gtagtcggcc agccggtggt ggcgggggcg accgttacag cggaaatcat
      421 ccgccagggt cgcaccagat cgatcacggt cttcaagaag caccgtcgca agaattatcg
      481 ccgtaccaga ggccaccggc agggctttac gcagctgcgg gtcactggga tcgaaacgaa
      541 gtaacgactc gaaggggagt ttgccatggc aacaaacaaa ggcggcggtt cgacaaggaa
      601 cggccgtgac agtaacccac aatacctcgg tgtcaaagcc tacggcggag agacgatcaa
      661 ggcaggctcc atcattgtgc gccagcgggg aacgaagttt ttcccaggcc tcaacgtcgg
      721 tttgggccgg gatcacaccc tctatgccta tgtgtccggt gtcgtgaagt tcgaaggtgg
      781 ccgcggccgt cagaaggtca gcgtctatcc cgtcacagcg aaggcctgat ccctcctcag
      841 tccatacctt tgttcctacg gaacaggtgc cttccgttac agcatccccc atgagccggc
      901 gaatacgcta tactgctgtt tctgaataac caggccggct cggggatcgc tgccatgtct
      961 acctttgtcg atcaagtcca aatcctggtg aaggccggtc acggcggcaa cggcgcctgc
     1021 agctttcggc gggaaaagtt cgtccctcgc ggcggccctg acggcggtga cggcggtgac
     1081 ggcggcagcg tcgtggttga agccaccact cgcctcagca ccctgctgga tcttcgttac
     1141 caaaaacatt acgaggccga aaaaggcgag accggaggag gcagcaactg ccatggacgc
     1201 cggggcgccg acgtgcgcat tcccgtcccg gtcggcacca tggtgttcga cgagaacacc
     1261 caggaactgc tggccgatct cacgaaagac ggagaaacct gcgtcattgc caaaggcggt
     1321 cagggaggcc gaggcaacac ccaattcgcc acatcgacca accgggtgcc gacacaattc
     1381 gagccaggca ctcccggcga agagcgattg cttcgactcg atctcaagct cctggccgat
     1441 gtcggattgg tcggctatcc gaacgccgga aaatcgacac tcatcgccgc cgtgtccgcc
     1501 gctcgcccca aaattgccga ttaccccttc accaccctga cgcccaactt gggcgtggta
     1561 cggtggaccg gcgagcagac ctttgtcatc gccgatattc caggattgat cgaaggggca
     1621 catgaaggca aggggctggg atttcagttt ctgcgccaca tcgagcgaac cagcctgcta
     1681 ctccatgtga tcgacatttc agaatgggcc accgaggacc cggtcgcgag cctggagatc
     1741 atgcgccacg aactgaccgc ttacgatgac acattggcgg cgcgcccctt tgccgtcgtg
     1801 ggcacaaaac tcgacgtcaa aggcgagggg gaacggctcg aacggctccg gaagtactgt
     1861 cagcgacgca agattccatt ttttgcgatt tcggcagcca cgcgagaagg actggacgag
     1921 tgcatacgct acataggcca gcaagtggaa ctgcttcgga aaaccccgtg cgagacgaac
     1981 tcctaagaca agccaaacgc gtcgtcatca agatcgggag cagcctgatc gcctcgcgcg
     2041 aaaccggcct gagcgccgag cggatcgagc gccttgccgg cgaaatcgcc gaggtgcggg
     2101 cacaggggcg cgaggtactg gtcgtctcat ccggcgcggt cgtatccggc atcaaaaaac
     2161 tcgggttgcg cgaatacccg aaaagcctgc cgctcaaaca ggctgctgct gccgtcggcc
     2221 aaagcctcct catgtgggcc tacgaaaagg cctttgagcg cctcacgctc cgcgtcgcac
     2281 aggtgcttct cacgcacgaa gacctcgccg atcggcgacg gtttctcaac gcccggcaca
     2341 ccctcaccac cttgatcaag ttcggggtga ttccgatcat caacgaaaac gataccgtcg
     2401 cggtcgagga aatccgcgtg ggggataacg atactctggc ggcgcaagtc gcccacctcg
     2461 tcgatgcgga tttactgatc atcctctcgg acgtcgacgg gctctttacc gtggaccccc
     2521 gcaaggaccc gacggccgcc ttgattccac tgatccccga gattacggaa gacatcgaac
     2581 agcgggccgg catgtcgtcc accttcgaag gcacaggcgg catggccacc aaagtacgcg
     2641 ctgccaagaa ggtgggggaa tacggcgtcg ccaccctgat tctaaatggc acccagacaa
     2701 gactccttcc gaacgtgctt tccgggcaac ccggcggcag cctgtttctc gcccgggaac
     2761 gacgcatgaa cagtcgcaaa cactggattg cgtttaccct gcgcccccgc ggccatgtgc
     2821 agctggacca gggggccgtc gaggcactgg tacgaaacgg caaaagcctc ctggcatcgg
     2881 gcatcctcga tattaccggc catttcgacg ccggcgatcc ggtgacctgc gccggtcctg
     2941 acgggaaaga atgtgctaag ggcctggtca atttctcatc agagatcctg acgcgaatca
     3001 aggggctcaa gacggctgac atccaaaaaa tccccggtct tcaggagtat gaagaggtca
     3061 ttcaccgcga caacctggtg atcctttagc cagccgacga ccacccatac atggctcggg
     3121 cgcgatgtca ccaacacccg atctgaatac cggtccgccg cacacagacg tttgcatgga
     3181 gcccgtacaa tatgcgcctg ggactcttgg ggggcagttt taatccgatc caccggtgcc
     3241 acctctcgat tgcccgatct gcccgacagc tcctgcaact ggatcgcgtc ctgttcatcc
     3301 cgaccggtga tcccccgcac aaacaaccag gcacgctggc cgcagcctcg caccggcacc
     3361 gcatggtcca gctcgccatc caggacactc cagagttcgc tctcacggac attgaaatcc
     3421 aacgttccgg caaatcctat tccatcgaca cggtccgcgc gatccagcag gaatacggcc
     3481 ccgacaccgt cctctttttt attatcggac tggatgcgtt tctggatctg ccctcctgga
     3541 aagaagcgga caccctcctg aagagctgtc acttcgtcgt catttcaaga ccgtccacga
     3601 gctttcaggc gatggccgca atcccgctct tccgggacgt gccgcaaacc accctaaccg
     3661 cgctggacga agtgcgacaa gaacgcgcgg acgtggccat cacaaacggg caggctctga
     3721 cattcctgcg gttgcccccc tgcgacgtgt ccgcctcgga gatcagggca cggctgcgca
     3781 gcggcgcctc tctggcaaat ctgttgccca cccctgtcga atcctatata cttcgcgagg
     3841 ggttatacag ggaggacagt gagcgtctct gaatcaaagg ccaaagctct cgcggtggca
     3901 agcgccatcc tggacaaaaa agccaccgat gttctcatcc tgcatatcgc cacactgacc
     3961 tccgtcgccg actatctggt catcggttcc ggggaatcgg agcgtcaagc gcgggcaatg
     4021 gcggaccacg tgagcgacgt gctcacggcg caaggtcacc ggccgctcag cactgaaggt
     4081 gcatcgtcag ccaagtgggt cgtcatggac tttggtgacg tgatcgtcca cattttccaa
     4141 aaggatattc gcgaacacta cgccctcgaa cgactgtggg gcgacgcggg acaagttcga
     4201 ttgccggaag agcgggccgt gaaagccgct cccccgacac gaaccgccac acgtccggcg
     4261 cgcacccgga aacgggtcta gcatgtttcg gctcctctcc acgatcttcc tcgtc
//
LOCUS       DAOQRM010000195         4307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_21921_length_4307_cov_13.912747, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000195 DAOQRM010000000
VERSION     DAOQRM010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21921_length_4307_cov_13.912747"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1187)
                     /locus_tag="PKK81_11965"
     CDS             complement(84..1187)
                     /locus_tag="PKK81_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV33647.1"
                     /translation="MSLSPLRFAVVGLGHISQVAVLPAFAHAKSSARLTALVSDDPLK
                     RRLLGRQYGIAHTYAYKDYEQCLRSGEIDAVYIALPNSLHREYAVRAARAGIHILCEK
                     PLAVTAADCRKMVRAAERYRVKLMVAYRLHFEACNLQTIALLQSGRLGEPRLFHSVFT
                     QQVRPGDIRLSQRLGGGPLYDIGIYCINAARYLFRDEPTEVTALVGRGHDRRFQKVEE
                     SAGAILRFPHDRLATLTCSFGATDAAMYEIVGTKGRVRVEPAYEYLGSLKASITVNGK
                     TRVRTFKPGDQFAPQLIYFASCVQDNRQPEPNGLEGLLDVQIIEALHRSARQGRSVPL
                     RLSTKQVRPDVRLQMHRPPVVKPKQVRATSPTL"
     gene            complement(1297..1695)
                     /locus_tag="PKK81_11970"
     CDS             complement(1297..1695)
                     /locus_tag="PKK81_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV33648.1"
                     /translation="MANTPTISNTDGAVKLVRDDHRHILALFQLYRATPADSRQSYVE
                     QILQRLSDHFHMEERLTEDLRLHGDEGRILVEQLLVEHEEIKAMIDELQQAENDDDES
                     LDAFFEDMMQTVRAHFIAEERDLFPLLNKG"
     gene            complement(1872..3347)
                     /locus_tag="PKK81_11975"
     CDS             complement(1872..3347)
                     /locus_tag="PKK81_11975"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNV33649.1"
                     /translation="MGRYTQPVLPALCAVLLLGNACSHSIPQSTSKPVPPPITSAVPS
                     FAAMAKEESRAPLSQMPFQVKTDLTPIQTAIRDAIPERITEAGHPLGQEFRWTFVRSS
                     NPQVHIQDGLVAIHAEYKGEIESRGSSRACRLDPVFATLDVTGKLALLQESESVSFGF
                     DPTHLAVRTKPESDARCNMFNVPVTDQAPDLFGLPEIKIALTDAVHADAFAIPLQRLW
                     DDLDGPLSLSMATLNTHACLYGNPREMILGQQKGTTQDTVILGSAKQMPFITYEPTCT
                     EAAPTVALVNSGPLSAVNKPYTMLARVPFSYQQLSHQLQSKLFHQTVVLDSTASDRAV
                     IEQVSAADASGRVLVTVELSGDLKGTIYYWGTPRLDDGGRSLSVPDLQMANESKSAID
                     SIRIGYWQLVDRELNQKLRQAMAVDISAQVDRLKQTLTGTHRSGGVTMDILVTRQMPD
                     QVRSSPQGLTVIILLEGTANATGRVTLEGQRTRALLPRESR"
     gene            complement(3638..3952)
                     /locus_tag="PKK81_11980"
     CDS             complement(3638..3952)
                     /locus_tag="PKK81_11980"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNV33650.1"
                     /translation="MIAHLTRFVFAVTMLSLVGCQSTTGKTAGQTIDDATITASVQSK
                     LSSDRLSNFSRIDVDTERGVVTLNGVVTSTEQKMRVAELTRGVTGVRTVNNNLQIQPQ
                     PQ"
     gene            complement(4023..>4307)
                     /locus_tag="PKK81_11985"
     CDS             complement(4023..>4307)
                     /locus_tag="PKK81_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNV33651.1"
                     /translation="GAFIGAGIALLLAPQSGTELRSTLRDYASKAKDELDEATEQGRA
                     AWDHAVDRGQEYVEGGKQTLRNAGRAAREFVDETVQTAEKTVGEAASRRA"
BASE COUNT          836 a         1224 c         1313 g          934 t
ORIGIN      
        1 gtaacggtct gcgttccttg catgaactgg gcaaagccct agtcgcgccg gtggaggcat
       61 gactccatcc ccgacatgtg ccctcagagg gtgggggaag tcgcacgaac ctgtttgggt
      121 ttgacgaccg gcggtcggtg catctgcagc cggacgtcgg gacgcacctg tttcgtcgat
      181 agtcggagcg ggacgctacg gccttgccgg gcggaacgat gcagggcttc gataatttgc
      241 acatcgagca ggccctcaag cccattcggt tccggttggc gattgtcttg tacgcaggag
      301 gcaaagtaga tgagttgggg ggcgaactga tcccccggct tgaacgtgcg gacgcgtgtc
      361 ttcccgttta cggtgatgga agctttcaag cttcccaggt attcgtaggc cggctccaca
      421 cgcacccggc cttttgtgcc gacgatttca tacatggcgg catcggtcgc gccgaagctg
      481 caggtcaatg tcgccaggcg atcgtgagga aagcgaagga ttgcgccggc cgattcttcg
      541 accttttgga atcggcggtc atggccgcga ccgaccaagg ccgtgacttc cgtcggttca
      601 tcgcggaaga ggtatcgtgc cgcgttgatg caatagatgc cgatatcata cagcggcccc
      661 ccgcccaacc tctggcttaa acggatgtcg ccggggcgaa cctgttgggt gaagactgaa
      721 tgaaagagcc tcggctcacc taagcgcccg gactgcagca gggcaatggt ctgaagatta
      781 caggcctcga aatgcagacg atacgcgacc atcagtttga cgcgataccg ctcggcggcc
      841 cggaccatct tccggcaatc ggcggccgtc acggcaaggg gcttttcaca caggatatgt
      901 atgccggcac gagctgcccg cacggcgtat tcgcgatgga gactgttcgg cagggcaatg
      961 tacacggcat caatctcgcc gcttcgcaaa cactgttcgt agtctttgta ggcataggtg
     1021 tgggcgatcc cgtattgtcg gccgaggagg cgacgcttca gggggtcgtc cgataccagc
     1081 gcggtcagcc gtgcggagga ttttgcatgc gcgaaggcgg gaagcacggc aacctgggaa
     1141 atatgtccaa gaccgacgac cgcaaaacgc aagggtgaaa gactcattca gtgccactcc
     1201 atttctcccc tcccggggaa ggggatgtca tcatccatcg ttcgacccgt caccctgtgt
     1261 agcattccgg atgtgctgct accgatgggc gtggtgctat cccttgttca atagaggaaa
     1321 gagatcgcgc tcctccgcga tgaaatgggc acggacggtc tgcatcatgt cttcaaagaa
     1381 tgcatccaac gattcgtcgt catcgttttc agcctgttgt aactcgtcta tcattgcctt
     1441 gatctcctca tgttccacga ggagttgttc cacgaggata cggccttcgt cgccgtgaag
     1501 ccgaaggtcc tcagtcaacc gctcttccat gtggaaatgg tctgataatc gttgcaggat
     1561 ctgctcgacg tacgattgtc gggagtctgc cggtgtggca cggtacagct ggaaaagggc
     1621 cagaatatga cgatggtcgt cgcgtaccag cttgactgcg ccgtcggtgt tcgaaatcgt
     1681 cggtgtattc gccatggggc tgcctcccaa tgagtgacgc gtggtcgttc gtgagtctca
     1741 acgccaccgt atcagtcaat cgttcgcgtg cgcactgggc aggaccctaa tcatcgacgc
     1801 cacggtgagc ggcgacattc ggagcgcgga gaggaagcgt cgttggacgc ctcctctccc
     1861 tgtggtgcga gtcagcggct ttcccgcggg agcaacgccc tggtcctttg tccctccagt
     1921 gtgacccggc cggtggcgtt ggcggtccct tccagcaaaa tgatgaccgt caggccctgc
     1981 ggcgaagagc ggacctggtc gggcatctgc cgtgtcacaa ggatatccat cgtgacgcct
     2041 cccgaccggt gtgttccagt cagtgtttgc ttcaatcgat cgacttgcgc agagatgtcg
     2101 accgccatag cctgccggag tttctgattg agttcccgat ccaccagttg ccaatagccg
     2161 atacgaatgg agtcgatggc ggatttcgac tcattggcca tttggaggtc cggcacggag
     2221 aggctccgtc ccccgtcatc caggcgtggc gttccccaat agtagatcgt ccctttgaga
     2281 tcgccgctga gttcgaccgt caccaggacg cgcccactgg cgtccgcggc ggagacctgt
     2341 tcaatgacgg ccctgtctga ggccgtggaa tcgagcacca ccgtctggtg gaacagcttg
     2401 ctctgcagtt ggtgcgagag ctgctgataa gaaaatggga ctcgggccag catcgtgtag
     2461 ggcttattaa cagccgacag agggcccgaa ttgacgaggg cgactgtggg agcggcttcc
     2521 gtacaggtcg gctcataagt gatgaagggc atttgttttg ccgagcccag aatcaccgtg
     2581 tcctgagtcg tacctttctg ctgtccgaga atcatttctc tcggattgcc gtagaggcag
     2641 gcatgggtgt tgagtgtcgc catggagagg gacaggggac cgtcgagatc atcccagagc
     2701 cgttgcaatg gaatcgcaaa cgcgtcggca tggacggcgt ccgtcagggc gatcttgatc
     2761 tcaggcagtc cgaacaagtc gggagcctga tcggtcaccg ggacgttgaa catgttgcag
     2821 cgcgcgtcgc tctccggttt ggttctcacc gcgagatgag tggggtcgaa tccgaaagac
     2881 accgattcgc tttcttgcag cagggcaagt ttgcccgtga cgtccagcgt ggcaaaaaca
     2941 ggatccaggc gacaggcccg tgaactgccg cgcgattcga tctctccttt atattcggca
     3001 tgaatcgcga cgaggccatc ctggatatgc acctgtggat tgctgctcct gacaaaggtc
     3061 caacggaatt cttgaccgag cggatgcccc gcctccgtga tgcgttccgg aatggcatct
     3121 cgaatggccg tctgaatcgg cgtcagatcg gtcttaacct ggaagggcat ctgcgagagc
     3181 ggagcccgtg actcctcttt cgccatcgct gcgaaggaag gaaccgcgct ggtgatcgga
     3241 gggggaaccg gtttggatgt tgactgcggg atggaatggc tgcaggcatt accgagcagc
     3301 aggactgcgc agagcgccgg tagaacaggt tgtgtatatc gacccatatt gaacctcctt
     3361 aatccatgcg tgaccgcagc cacccaagaa gcggatgtgg ctaggcctgc gtgcgaggcg
     3421 tacagagggc tggcacttac aatgtgccac gcgttatctg tgcactcccg ctctttcgac
     3481 caccgatgac accacgaact gacgagatcc tgcctggacc ctcacgaggc tcccgtcgat
     3541 tgtgagattg catgcagcct cagaggtgag aggtagagac ctgcctgacg tctccgcgaa
     3601 tccccacatc ctgtacgcgc cagtggcttg cttcttctca ctgtggttgc ggttgaattt
     3661 gcaggttgtt gttcacggtc ctcacgccgg tcactccacg tgtcagttcc gctacccgca
     3721 ttttctgttc ggtggatgtg acgacaccgt tcagagtgac caccccgcgt tccgtatcca
     3781 cgtcgatacg ggaaaaattc gacaagcgat cggaggatag tttggattgc accgaagccg
     3841 tgatggtggc gtcatcgatg gtttgtcccg cggtcttgcc ggtcgtggac tggcagccga
     3901 ccagcgagag catcgtcaca gcaaaaacga aacgtgtcag atgtgcgatc attatgggta
     3961 ctccttttca gaatttcgtc caggtgtata tccgtatgcg ctcagaccat gtcgtacgaa
     4021 tactacgcgc gccgggaggc cgcctcgccc acggttttct cggcggtctg gacggtctca
     4081 tccacgaatt cccgggcagc ccgtccggcg tttcgcagcg tttgctttcc accctcgaca
     4141 tattcctgcc cgcgatccac ggcgtgatcc caagcggccc gaccctgttc ggtagcctcg
     4201 tccagttcat ccttggcctt ggatgcatag tcgcgcaggg tggagcgaag ctccgtgccg
     4261 gactggggcg ccaaaagtag tgcgatcccc gcaccgatga atgcgcc
//
LOCUS       DAOQRM010000196         4299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_21987_length_4299_cov_13.361451, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000196 DAOQRM010000000
VERSION     DAOQRM010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21987_length_4299_cov_13.361451"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKK81_11990"
     CDS             <1..191
                     /locus_tag="PKK81_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33652.1"
                     /translation="METYLAAKELILDYPALRSPQLLHLALALELKPLRLTVVSADKQ
                     LLAACRPAGLHIINPEDD"
     gene            complement(630..1148)
                     /locus_tag="PKK81_11995"
     CDS             complement(630..1148)
                     /locus_tag="PKK81_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33653.1"
                     /translation="MRRTLVACQILAVALIVCIAGNGQAQVMGEEAELDRLRAKAEDA
                     MGNDDAETAAMSMGRAALMAAQLSKRQTEPAPRQTFNATEHLYRSQEHGYRAIALFRR
                     AGGELPASAGVCGSLQLAQLELRHAQEALSGPNDTEGKNTASPRRKAAQQSMEDWSIV
                     LDSIQGEFRCPS"
     gene            complement(1187..2263)
                     /locus_tag="PKK81_12000"
     CDS             complement(1187..2263)
                     /locus_tag="PKK81_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV33654.1"
                     /translation="MRCILSLITLGALVLLGDQSWARRDALTAEQKSQLERIDRVLVD
                     VIVLSDKGSLDAKPLVDVVVTRMKEFDYTTVTDPTQPHDVELKIKCEQRKTWEGTTTM
                     GSDADLPDAPSRIWKGPACQLGYLLNGKKMAWRKEVRTDFDDAQAAATLAKAGDPTAY
                     AMAKLQARLEEYDFPALITAEWGQEARLFRLYDDPKTPIARKVKLVALFGDLFSVKAI
                     PRLLDGLNGADRSIAKASALALGNIGQKDTIPVLIKTMKSGPPDLRAPAAKALGIVGA
                     LHGDFTIVDPLLETLNTEDVAVKTEVAWALGKLPDMRAYEPLFTLQKSLYRVHENDAD
                     PNLVKLKEAVNWSIKQIDTWEHVQ"
     gene            2378..3208
                     /gene="proC"
                     /locus_tag="PKK81_12005"
     CDS             2378..3208
                     /gene="proC"
                     /locus_tag="PKK81_12005"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNV33655.1"
                     /translation="MLTGTRIAFIGGGNMAEALLAGLLRKGVAVAEHLTVSDPDVHRR
                     ELLAERFGVAVHADNRTAAQGAELVVFCVEPQVLDSVLDEVASSLQSRPLVMSVAAGY
                     PISRLQAHLKTATRLVRAMPNTPSTIGEGVTAMSMMPGLSSEDQDRARCLFESVGQVV
                     VVEERLMDAVTGLSGSGPAYVFAMIEALADGGVLMGLPRITAQVLAAQTVAGAARMVL
                     DQGTQPAVLKDRVASPGGTTIAGLSRLEQGRLRATLMSAVTAAAQRSQELGKEESHHL
                     "
     gene            3224..3418
                     /locus_tag="PKK81_12010"
     CDS             3224..3418
                     /locus_tag="PKK81_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33656.1"
                     /translation="MQYWVKVVFADNQELLVKDAIRHTISEDMEVLEVDSAKEVIIVP
                     MKQIKYIACDATVFAQKSKT"
     gene            complement(3415..>4299)
                     /locus_tag="PKK81_12015"
     CDS             complement(3415..>4299)
                     /locus_tag="PKK81_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNV33657.1"
                     /translation="FGTSQLSAISRPRIKAWAAGLLEKGLDFDTALNALLTLSAVLTE
                     AVEDGLITHNPALRSGKLLKRPATIEESELAIFTPEEEGLLLETIRRERPLFFPMALT
                     FFRTGLRAGEVLGLHRGDLNFHSRSIYVQRNWSRGILGTPKNGKARRVDMSQGLAKAL
                     TEWSELQDLEAAAAGQAAPEILFPGNLGGTRREPSYMAENWLRYKLWFPMVAKAGLRR
                     LDMHSVRHTYASRLIANGENLKYVQEQLGHASITITCDTYGHLIPGGNRQAVDKLDSL
                     KIGFGTVTKTVTNASENC"
BASE COUNT          786 a         1262 c         1329 g          922 t
ORIGIN      
        1 cgatggagac ctatctggct gcgaaggagt tgattctgga ctatccggct ctgcgctccc
       61 cgcagctgct acacctggcc ttggcgttgg aactcaagcc gctccggctg acggtggtga
      121 gtgcggataa gcagctcctg gcagcctgtc gtcccgccgg gctgcacatt attaatccgg
      181 aagacgacta gcgcgtagca ggatgcggaa acagcccgcc agcggcgttc tcgcttcgtt
      241 caggccctca acgtaccgca agggtacgcc tcgggccttc actcgctgcg gccttgctgg
      301 acggcctgtt tgcgcatcct gcagacgatc cgtccgtcaa cgtggctagt gagttaatcc
      361 cgaccgcttg cgggtaaaac acgttcttcc gcagcctttc agggcgtgat ggctaggcag
      421 attgctaatg agtagccttt ttcaacggat ggctggcccg gattattcat gaatgccgtc
      481 gcgaggcgtt tgagaccgaa gcccgccggg gcttctcgca agtgaggccg acgcaggcgt
      541 acgtgcagtc cgtcgaggag gccgaacgag aacagccccg ccgggctaga ggttcggacg
      601 ccggagtagg tattcatgaa tagtccgggc taggaggggc agcgaaattc cccttgtatc
      661 gaatccaaca caatcgacca gtcttccata ctctgctgcg ccgccttccg tcgaggggag
      721 gcggtgttct tcccctccgt atcattcggc ccgctgagag cttcctgcgc atggcgcagt
      781 tcgagttgtg ccagttgaag actgccgcag acgccggctg aagcggggag ttccccgccg
      841 gctcgccgaa acagggcgat cgcccgatat ccatgttcct gcgaccggta gaggtgctcc
      901 gtcgcattga aggtctgccg cggggccggc tcggtctgcc gcttggagag ttgtgccgcc
      961 atcaatgccg cgcgtcccat gctcatcgcc gcagtctccg catcgtcatt acccatggca
     1021 tcttccgcct tcgcccggag gcggtcgagt tccgcctctt cccccatcac ctgagcctgc
     1081 ccgttgccgg cgatacagac gatgagcgcc accgccagga tctgacaggc cacgagggtc
     1141 cgccgcatgg ccgacctgcc gaagcagagg aagaaggtgg agcctgctat tgcacgtgtt
     1201 cccaggtgtc gatctgtttg atgctccaat tgacggcttc cttcaatttg accaggttgg
     1261 gatcggcatc gttttcgtgc acgcggtaga gcgatttttg gagcgtgaac aacggttcat
     1321 acgcccgcat gtccggcagt ttcccgagcg cccaggcgac ctcggttttc acggcgacat
     1381 cctcggtatt caacgtttcg agcaggggat ccacgatcgt gaagtctccg tgcaaagcgc
     1441 cgacgatgcc cagggctttt gcggccggcg cgcgcaaatc cggggggccg gatttcatcg
     1501 tcttgatgag caccgggatc gtgtctttct ggccgatatt gcccagcgcc agggccgagg
     1561 ctttggcgat actgcggtcg gctccattga gtccgtcgag gaggcgaggg atcgccttca
     1621 cggagaacag gtcgccgaac agggccacca gcttcacctt gcgcgcgatc ggagtcttgg
     1681 gatcgtcgta gaggcgaaac aggcgcgctt cctgtcccca ttcagcggtg atgagtgcgg
     1741 ggaaatcgta ctcttccagt cgcgcctgta gcttggccat cgcgtaggcc gtgggatcgc
     1801 cggctttcgc gagcgtggcc gcggcttggg cgtcgtcgaa atctgtccgg acctctttgc
     1861 gccaggccat ttttttcccg ttgaggagat agccgagctg acaggccggt cctttccaga
     1921 ttcgtgacgg cgcatcggga aggtcggcat cgctccccat cgtggtggtg ccctcccagg
     1981 tctttcgctg ctcgcatttg atcttcagtt ccacatcgtg cggttgggtc ggatcggtca
     2041 cggtcgtgta gtcgaattct ttcatgcggg tgacgaccac gtcgaccagg ggtttggcgt
     2101 cgaggctgcc cttgtccgag aggacaatca catcgaccaa cacgcgatcg atgcgttcca
     2161 gttgactctt ttgttcggcg gttaaggcgt cgcggcgggc ccaactctgg tcgcccagga
     2221 gtaccagtgc ccccagggtg atgagcgaaa ggatgcatct catgtctgct ccattttgat
     2281 gaaaagacga tgatcacgaa ccctgccgcc actctataca accccttccg gctgttgcaa
     2341 gttgcgcctg tcggagagcc gtccgtataa tcgcgccatg ttgacgggga cacgaatcgc
     2401 gttcatcggc ggcggcaata tggccgaggc gctgctggca ggtctcctgc gcaagggcgt
     2461 ggctgtcgcc gagcacctca ccgtcagcga tcccgatgtc catcgccggg aactgctggc
     2521 agagcgattc ggggtcgcgg tccatgccga caaccggacg gcggcgcaag gtgcggagct
     2581 cgtcgtattc tgtgtcgaac cgcaggtgct cgattctgtt ctggatgagg tggcttcaag
     2641 tctccaatcc cggcccctcg tgatgtccgt tgcagccggg tatccgatct cccgcctgca
     2701 ggctcacctc aagaccgcaa ccagactggt ccgtgccatg ccgaacacgc cctcgaccat
     2761 cggagaaggg gtgacggcga tgagcatgat gccgggtctc tcatccgaag atcaggaccg
     2821 ggcacgatgc ttgttcgaat cggtcggtca ggtggttgtg gtggaggagc ggctgatgga
     2881 tgccgtgaca ggcctgagcg gaagcggacc ggcctacgtg ttcgccatga tcgaagcatt
     2941 ggccgacggc ggcgtgttga tgggactacc tcgaatcacc gcgcaagttc tggcggcgca
     3001 gacagtagcc ggcgcggcgc gtatggtgct ggatcagggg acacaaccgg ccgtgttgaa
     3061 agatcgcgtg gcttcgccgg gcggcaccac gattgcggga ctctcccgcc tggagcaggg
     3121 gcggctgcgg gcgaccttga tgtcagccgt gaccgccgcg gcgcagagat cgcaggaact
     3181 gggaaaagaa gagagccatc acctctaaca aggagcgtgt ctcgtgcaat attgggtgaa
     3241 agtcgtgttt gccgacaatc aggaactact ggtgaaggat gcgattcgtc acacgatcag
     3301 cgaagacatg gaggtgctgg aagtcgattc ggccaaagaa gtgatcatcg tccccatgaa
     3361 acagattaag tacatcgctt gcgatgccac tgtattcgct caaaaatcaa agacttagca
     3421 gttttctgaa gcgttggtca cggttttggt cacggttcca aaaccgattt ttaacgaatc
     3481 cagcttatcg accgcttgtc gattcccgcc tggaatcaga tgcccatagg tgtcacaggt
     3541 gatggtaatc gacgcatggc ccagttgctc ttggacatat ttcaggttct cgccattcgc
     3601 gatgagtcgg cttgcatagg tatgtcggac actatgcata tcgagtcgtc gtagtcccgc
     3661 tttcgccacc atgggaaacc acagtttgta ccgcaaccaa ttctcggcca tatagctcgg
     3721 ctcccgtctg gttccgccaa ggttgcctgg aaagagaatt tcaggtgctg cctgcccagc
     3781 ggcggccgcc tccaggtcct gtagctcgct ccattccgtc agagcctttg cgagaccctg
     3841 cgacatgtcc acccgtcgag ccttgccgtt cttaggtgtg cccagaattc ctcgtgacca
     3901 gttccgttgc acgtagatgg atcggctgtg aaagttcaga tcgccccgat ggagtccaag
     3961 cacctctccg gctcgtaggc cggtgcgaaa gaaggtcagg gccatcggga aaaacagcgg
     4021 gcgttcgcgt cgtattgtct ccagcaacag gccttcctct tctggcgtga agatcgccag
     4081 ttcggattcc tcgatcgtcg ccggccgttt cagcagtttg ccggaacgga gggccgggtt
     4141 gtgagtgatc agcccatctt cgacggcttc ggtcaacacc gcgctgagcg tgagcagcgc
     4201 attgagtgcc gtatcgaaat ccagcccctt ttcaagcagc cctgcggccc atgctttgat
     4261 ccgtggtcgt gagatggcgg agagttgaga ggtgccgaa
//
LOCUS       DAOQRM010000197         4273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_22184_length_4273_cov_14.516358, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000197 DAOQRM010000000
VERSION     DAOQRM010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22184_length_4273_cov_14.516358"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..527)
                     /locus_tag="PKK81_12020"
     CDS             complement(87..527)
                     /locus_tag="PKK81_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33658.1"
                     /translation="MSVDRSLVRLILVIVWLNGVHTLDHMIRGDFHWPLDGQSVGFVL
                     VVAAIYLVIGMGIRLSNRGVVGPLFWAIIGGGGLVFGWLSHFSPFTDQPLHVIYGSYH
                     NATGGALAVLCLMLLMGVVLTATLYSIYLWHHQRHITVSRPVSR"
     gene            complement(524..970)
                     /locus_tag="PKK81_12025"
     CDS             complement(524..970)
                     /locus_tag="PKK81_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNV33659.1"
                     /translation="MTGEGSEEWLARLIRECRTIAVVGLSSNPARPSYRVAAYMQQQG
                     KVVVPVNPRESDLLGERAYPALSAVPGPVDMVNIFRRSEEAGAVVDEAIRIKAKAVWL
                     QEGVIDEAAAERARRAGLQVVMDRCWLKEHMRCVSGDGPDVRPANV"
     gene            complement(967..1590)
                     /gene="msrA"
                     /locus_tag="PKK81_12030"
     CDS             complement(967..1590)
                     /gene="msrA"
                     /locus_tag="PKK81_12030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNV33660.1"
                     /translation="MNIVIRRGMYTWLVLAMVLAGSLASVGQAAGPAGPAKAYFAGGC
                     FWCMEEVFEKVPGVISVTSGYMGGRVEHPSYEQVSAGGTGHAESVEVVYDPAKVSYTA
                     LLDAFWHNVDPVTPNAQFCDHGSQYRAVIFYQGDEEKRLAEESKGAIEQSGRLPGRIV
                     TELTLASTFYPAEDYHQDFYKKNPVRYKFYKYNCGRAQRLEDLWGAP"
     assembly_gap    1850..1949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1950..2447)
                     /locus_tag="PKK81_12035"
     CDS             complement(<1950..2447)
                     /locus_tag="PKK81_12035"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33661.1"
                     /translation="MRYIVGVMGPAKARKKDVDNARVLGELIARREWVVLTGGRDVGV
                     MDAACQGAKKVPGSLTIGVLPSARERVSKYVDLAIITEMGNARNNVNVMSSNVVVVCG
                     LMGAGTVSEVALALKAGKPVILVGATPAEEKFFKKLGGRLIASVDSPEEAIGLMMKQF
                     EQQQPD"
     gene            complement(2450..3271)
                     /locus_tag="PKK81_12040"
     CDS             complement(2450..3271)
                     /locus_tag="PKK81_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV33662.1"
                     /translation="MLKTDYVFHATEEARELERLQMLERIFDPGTQRRMLATGLTTGW
                     HCLEVGAGAGSIVRWLEQRVGPSGKVVAVDTNPRFLRGSGSSTIEILQGDICDMELPP
                     AAFDLVHARYVMIHIADYQTAFERMLRCVKPGGWVVIEEPDFQAARAVAGPDEARAAF
                     GRVSEAIERMFTSLGMDHALGAKLPVLFQRHHLDQVTVEHEGHLSAGGDLIPQMMKLS
                     AEQLQAKYVATGLVTESDMEQYGRLADDPDFWAIYYATVAVTGWWQPQCGGRTQG"
     gene            complement(3329..>4273)
                     /locus_tag="PKK81_12045"
     CDS             complement(3329..>4273)
                     /locus_tag="PKK81_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33663.1"
                     /translation="SPDPQRALDCAKRLPSLMPGAGHLVHMPAHIYMRLGRYREASER
                     NATAAMVDHEYLSRHPLEGNYASGYYAHNLHFLNASLMMEGRSAEALQVARDLLAKVP
                     GDEIAKEPSLEWYAPTVLLTMVRFGQWGELIRHPPPSKSLRLTTGLWHFSRGLAFAAT
                     TRFGSAEGELSNLRKAMKPFARAKTAEGKISRTMLKVAERVLVGEMAARRGQYEAGIQ
                     ALREALQLEASLPYSEPPFWVQPVRHNLGAVLLLAERPAEAEAVYREDLRLNPENGWA
                     LHGLMQSLRAQNKDATQEEARFRAVWAQADVTLTGSRF"
BASE COUNT          846 a         1429 c         1119 g          779 t
ORIGIN      
        1 gtcactattc catggagggg cggaatgctc aagaaaatga cacaaatcag ctcagaggga
       61 aatcgatgga agatctggga acgaggttac cgggaaacag gacgcgacac ggtgatgtga
      121 cgttggtgat gccacaggta gatcgaatag agcgtcgccg tcagcacaac gcccataagc
      181 agcatgagac ataacaccgc gagggcgcca cccgtggcat tgtgatagct cccgtagatc
      241 acgtgcaacg gctggtcggt gaagggactg aagtgggaca accacccaaa gaccaatcct
      301 ccgcctccga tgatggccca gaacaggggc ccgacaaccc ctctgttgga cagccgtatt
      361 cccattccaa tgacgaggta gatggcggcg acgacgagca caaaaccaac cgattgccca
      421 tcgagcggcc agtggaagtc tccccgaatc atgtggtcga gcgtatgcac cccgttcagc
      481 cagacaataa ccaggatcag gcggaccagg gaacgatcca cgctcatacg ttcgccggcc
      541 tgacatccgg gccgtcccca ctgacgcaac gcatatgctc cttcagccag caacgatcca
      601 tcaccacctg cagcccggcc cggcgcgcac gctcagccgc cgcctcgtcg atcacgccct
      661 cctggagcca gaccgccttg gccttgatcc ggatggcttc atcgacgacc gcgcccgcct
      721 cttcagatcg tcggaaaata ttcaccatat cgaccggacc gggcacagcg gagagtgccg
      781 gataagcccg ctcccccaac aggtccgact cgcgcggatt gaccggaacc actaccttgc
      841 cctgctgctg catgtaggcg gccacgcggt atgagggtcg ggccggattc gacgacaatc
      901 ccaccacggc aatcgtccga cattcccgga tcagccgcgc gagccattcc tccgagccct
      961 cacctgtcat ggagctcccc agagatcttc cagtcgctgc gcccgcccgc agttatattt
     1021 gtagaatttg taccggaccg gattcttctt ataaaaatcc tggtgatagt cctcggccgg
     1081 ataaaacgtc gaagccaggg tgagttccgt aacaatcctc cctgggagcc gcccggattg
     1141 ctcgatggcg cctttcgact cctccgccag gcgcttctcc tcgtcgccct ggtaaaagat
     1201 cacggcccgg tattggctgc catggtcgca aaactgtgcg ttcggcgtga ccgggtccac
     1261 attgtgccaa aaggcgtcaa gcaaggcggt gtaactcacc ttggccggat cgtagaccac
     1321 ctccaccgac tcggcatgac ccgttccacc ggcagagacc tgctcatagc tggggtgttc
     1381 gacccgcccg cccatatatc cgctggtcac cgatatgacg cccggcactt tctcgaacac
     1441 ttcctccatg caccagaaac accctccggc aaaatacgct ttcgccggac cggcaggacc
     1501 cgcggcctgc ccgaccgaag ccagtgaacc tgccagcacc atcgccagaa ccagccacgt
     1561 atacatgcct cggcgtatca cgatattcat agaataagtc ctccctgcaa ctgacccgcc
     1621 tcctcgctgt tcatcttacc aaagtacgtg agcgactgcg gtcgcagcgc caaagctata
     1681 cctcttatct acgagcatcg caatcgattg gatgtttctg tatgatgggc aggatgagac
     1741 gactggcttc gcgtgtggga ctggtggcgg tgctgacgct ttccggatgc gcctcctggt
     1801 tcatgaaagg ggaagcgccg gaagtcctcg tgaccaacgt cacgccgctn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tccggttgct gctgttcgaa ctgtttcatc
     1981 atgagcccga tggcttcttc cggactgtcc acggaagcga taagccgccc accgagtttc
     2041 ttgaaaaatt tctcctcggc cggcgtggcc ccgaccagaa tcaccggctt tcccgccttg
     2101 agcgcgagcg ccacctccga cacggtgccg gcccccatca gcccgcagac caccaccaca
     2161 ttgctggaca tgacgttgac gttgttgcgg gcattgccca tttcggtgat gatggccaga
     2221 tcgacatact tggacacgcg ttcacgagcc gaaggcagca ccccgatcgt caggctgccg
     2281 ggaaccttct tggcgccctg acaggctgca tccatcacgc cgacgtcacg accgccggtc
     2341 agcaccaccc actcgcgtcg cgcaatcaat tcgcccagca cgcgagcatt gtcgacatct
     2401 tttttcctgg ccttggccgg ccccatgact cctacaatgt atcgcatgcc tatccttggg
     2461 ttcgcccgcc gcattgcggc tgccaccagc cggtcaccgc caccgtcgcg taataaatgg
     2521 cccagaagtc cggatcgtcg gcaagcctcc catactgctc catatcggac tccgtcacta
     2581 gtcctgtcgc cacatacttc gcttgaagct gctcggcaga caatttcatc atctgcggaa
     2641 tcaggtcgcc gcctgcggat aaatgcccct cgtgttccac cgtcacctgg tccaggtggt
     2701 ggcgctggaa caacacgggc aacttcgcgc ctaaggcatg gtccatcccg agggacgtga
     2761 acatccgctc gatcgcctcg cttactcgcc cgaaggccgc ccgggcctcg tccggtccgg
     2821 ccacggcgcg agccgcttga aaatccggct cttcgatcac gacccaaccg cccggtttga
     2881 cgcaccgcaa catccgctca aacgccgtct gatagtccgc gatatgaatc atcacatacc
     2941 gcgcatgcac cagatcgaac gcggcgggcg gaagctccat gtcacagata tccccttgca
     3001 ggatttcgat ggttgagctg ccgctcccgc gcaagaacct cgggtttgta tcgaccgcga
     3061 ccaccttgcc ggatggcccg acccgctgct ccagccaacg cacgatggag ccggcccctg
     3121 ctcccacttc caggcagtgc cacccggtcg tcagtccggt ggctaacatc cgtcgttgcg
     3181 tgcccggatc gaagatccgc tccagcatct gcaaccgctc taattcacgg gcctcttcag
     3241 tggcatgaaa cacgtaatcc gtcttcagca tctcgtatcc tttgtctggc acagagatgg
     3301 gctccggggc ccgaaccggc gcgggactct aaaatcgaga ccccgtcaat gtcacgtcgg
     3361 cctgcgccca tacggcacga aaccgggctt cttcctgggt tgcatctttg ttctgagccc
     3421 gcaggctctg catcagtccg tgcaacgccc atccattctc ggggttgagg cgcaagtctt
     3481 cccgatacac ggcttcagcc tcggccggac gttccgccag cagcagaacc gctcccaggt
     3541 tgtggcgaac cggctgaacc cagaacggcg gctcgctgta gggcaacgac gcctccaact
     3601 gcagcgcctc gcgcaaggcc tgaattccgg cctcgtattg cccgcgccgc gcggccattt
     3661 ccccgaccag gacccgctcg gccaccttca acatcgtgcg actgatcttg ccttccgccg
     3721 tcttggcgcg agcgaatggc ttcatcgcct tccggagatt cgacaattcc ccctcggcgc
     3781 tcccgaatcg tgtcgtcgcg gcaaaggcca acccgcgcga aaaatgccag agacccgtag
     3841 taagccgcag actcttggac ggcggcggat gcctgatcaa ctccccccac tgaccgaagc
     3901 ggaccatggt cagcagcacg gtcggcgcat accattccag cgacggctcc ttggcaatct
     3961 cgtcgcccgg aaccttcgcc aggagatccc gcgcgacttg cagggcttcc gcgctccggc
     4021 cttccatcat caaggaggca ttcaggaaat ggaggttgtg cgcatagtat cccgatgcgt
     4081 agttgccctc aagggggtga cgagacaaat actcatgatc caccatcgcg gcggtcgcat
     4141 tgcgctccga cgcctcccga taacgcccca gccgcatgta gatatgcgcc ggcatatgca
     4201 ccagatgtcc ggcacccggc atcagcgagg gcaaccgctt cgcacagtcg agcgcgcgct
     4261 gagggtcggg cga
//
LOCUS       DAOQRM010000198         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_22419_length_4245_cov_19.298091, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000198 DAOQRM010000000
VERSION     DAOQRM010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22419_length_4245_cov_19.298091"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..400
                     /gene="ffs"
                     /locus_tag="PKK81_12050"
     ncRNA           301..400
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKK81_12050"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            482..2359
                     /gene="dnaX"
                     /locus_tag="PKK81_12055"
     CDS             482..2359
                     /gene="dnaX"
                     /locus_tag="PKK81_12055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNV33664.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQSHVVQTLMNSIATKRIAHAFLFS
                     GTRGVGKTTVARILAKALNCEQGPTGTPCNTCSNCQEITQGTSVDVVEIDGASNTSVD
                     DVREIRENVKFTPFRGQYRVYIIDEVHMLSNSAFNALLKTLEEPPSHVVFIFATTEIH
                     KIPATILSRCQHYNFRRISKAEIVQRLRHVADQDGLTIEDRSLMALARASEGSMRDGL
                     SLLDQIIAFGGNTIRHEDLEALLGAVPQERVRAMVEAVIQQDSAKALQVIAALLDQGH
                     DVRAYCADLVEYVRNMLVAAVVPSGPELRSLIEATEEDLTQLARDAERFTVEQLQELF
                     RMYAAAEDSLRVSAHPRFVLETAAVRATRLLRTAEVQPASSRLAVQPEKPAADRRIVT
                     QTPAQTQDKATPSPAPVVKTTGVKVSQDNVAKTSAAGGSTPKAPSVAPPREVAAPPAR
                     TSAVAPSAPVVPPASAAPVQQEPKATSAAEASAAAVEVNWEQFQEAVSTNHPNIAPFL
                     EMGRLVKIEGGLITLGFAKQATTARSMLEKEDNLQALAALGESLYGCAVRIRIVEVAE
                     QDPGAAPTMKQIRVAKEQEQRLILTQQAKAHPLVKQALEMFGGELAEVRTTAPAQEVQ
                     E"
     gene            2356..2679
                     /locus_tag="PKK81_12060"
     CDS             2356..2679
                     /locus_tag="PKK81_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNV33665.1"
                     /translation="MKSPFGNMSNILKQAQAMQEQMAKVQEQAATKTVSGTAGGGIVT
                     VTVNGAMDLLSVKIDPEVVKAGDVEMLQDLVVAAGNDALKKSREMMAEEMKAVTGGMK
                     IPGLF"
     gene            2716..3318
                     /gene="recR"
                     /locus_tag="PKK81_12065"
     CDS             2716..3318
                     /gene="recR"
                     /locus_tag="PKK81_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNV33666.1"
                     /translation="MSVDQQGLLAKLVRELVRLPGIGQKSAQRLAFHLLKAEREDAMR
                     LAEAIQAVKDGLSFCRQCRNIAEGELCEFCRDPKRDRSKILVIEEPSTLYAIERAGGY
                     RGLYHVLLGVLSPLDGVGPSDIRAEELLDRVKAGGVEEVIVATNPTIEGEATAIYLTR
                     LLKPHHVRVTRIAYGIPVGMDIEYADEVTLVKSIEGRRDL"
     gene            3459..3866
                     /locus_tag="PKK81_12070"
     CDS             3459..3866
                     /locus_tag="PKK81_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNV33667.1"
                     /translation="MKTPAKKTTVARRKPPKANGVKYPDILADLEGQRAAILAEAGVV
                     LTNPTGLEVFPDVSDQASAEADQHFSFRIRERERKLLKKIDEALGRFATQTYGICEGC
                     EGDIPYKRLKARPVTTLCIECKTSQEEAEKSPR"
     gene            complement(3934..>4245)
                     /locus_tag="PKK81_12075"
     CDS             complement(3934..>4245)
                     /locus_tag="PKK81_12075"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV33668.1"
                     /translation="KAKSELLASLALRPNSPEVVNNLGAVYWKLGEKKNALASFEKAI
                     LMRPNYAIARFNVAEAIADDNPRRAITEYETYLALVEGILEETDRAALAQKRVQALKH
                     Q"
BASE COUNT          886 a         1139 c         1368 g          852 t
ORIGIN      
        1 catgcgtctc ggcaacggga ccgtgggttc gaatcccacc ctctccgcca tcgaacagag
       61 ccctgcaagg ttgagaaggc gtgtgagttg gaattgccat cgcgaacgga cgcggtggga
      121 ttcgaacgag ggggatcgca ggggagatac tcccctgcgt ggggagacac gagggggcgg
      181 gccccctcag tgggagcgcg tgagcgcgac ttcgaatgcc aaccctcatc cgccatcaac
      241 gtttgctcga ccatcgcctc gcgaatcgag gggatgggat agatatcgta actgtaacgg
      301 ggccgggccc tgtgcaacag aaccctgtga accccgccag gtccggaagg aagcaacggt
      361 aagcggttcg ttctgtgtgc cgcaggatca cctggcccca cttcttcgat gacatctcgc
      421 gcggcgcgtc aggcgccgcc agcactgacg agcaccaccg ccccgacacg atcgacgcaa
      481 catggactac caagtttccg caagaaaata ccggccgggg acgtttgacg atgtcatcgg
      541 gcagtcccac gtcgtgcaga cgttgatgaa ctcgatcgcg acgaagcgga ttgcccatgc
      601 gtttctcttc tccggtaccc gcggcgtggg gaaaacgacg gttgccagga ttttggccaa
      661 agcgctgaat tgcgagcagg ggccgacggg aacaccgtgt aatacttgtt cgaattgcca
      721 ggagattacg cagggcacgt cggtggacgt ggtcgagatc gacggggcct cgaacaccag
      781 cgtggacgac gttcgcgaga ttcgcgagaa tgtcaagttt acgccgttcc gtgggcagta
      841 tcgggtctat attatcgacg aagtgcacat gctctccaat tcggcgttca acgctctgct
      901 gaaaacgctt gaagagcccc cctcccatgt ggtgttcatc ttcgcaacga cagaaattca
      961 taagattccc gcgacgatcc tgtcacgctg tcagcactac aattttcgcc ggatctccaa
     1021 agccgagatt gtgcagcggt tgcggcatgt cgccgatcaa gacgggttga ccatcgaaga
     1081 ccgcagcctg atggctctgg cgcgggccag cgaaggcagc atgcgtgacg ggctcagctt
     1141 gctggatcag attattgcgt tcggtggcaa cacgattcgc cacgaagatc tcgaagcgtt
     1201 gctgggcgcg gttccgcagg aacgtgtacg ggccatggtc gaagcggtga tccaacagga
     1261 cagtgccaag gcgttgcagg tcatcgcggc gttgttggat caaggacatg acgtgcgcgc
     1321 ttactgcgcc gatctggtgg aatatgtgcg gaatatgctg gtggcggcgg tggtgccgtc
     1381 agggcctgag ttgcggagtc tcatcgaagc gacagaagaa gatttgacgc agctggctcg
     1441 tgacgccgag cggttcaccg tcgagcaatt acaggaattg tttcgaatgt atgccgctgc
     1501 ggaagacagt ttgcgagtca gtgcccatcc gcggtttgtg ctcgaaacgg cggcggttcg
     1561 tgccacccgg ctattgcgaa ccgctgaagt ccaaccggca tccagccgcc tcgcggttca
     1621 gcctgagaag ccggcagccg accgccggat cgtgacacag acgcctgcgc aaactcagga
     1681 taaggcgaca ccgtctcctg cgcctgtggt caaaactact ggtgtgaaag tcagccagga
     1741 caacgtcgca aagacgtcag ctgctggtgg gagtaccccc aaggccccct ctgtcgcacc
     1801 gccgcgcgaa gtggcggctc cacctgctcg cacgtctgct gttgcccctt cggctcctgt
     1861 ggttcctccg gcttccgctg cgccggttca gcaggaaccc aaggcgacct ctgcggccga
     1921 ggcgtcggct gccgcggtcg aagtgaattg ggagcagttc caagaggcgg tctctacgaa
     1981 tcatcccaat atcgcgccct tccttgaaat gggtcgtctg gtcaagatcg agggtgggtt
     2041 gatcacgttg gggtttgcca agcaggccac gaccgcgcgg tcgatgttgg agaaggaaga
     2101 caacctacaa gccttggcgg cgttgggaga aagtctctac gggtgtgccg tacgaattcg
     2161 aatcgtcgag gtggcggagc aggatccagg ggccgcccct acgatgaaac agatccgggt
     2221 tgcgaaggag caggagcaac gcctgatctt gacgcaacag gcgaaggcgc atccccttgt
     2281 taaacaagcc ctggagatgt tcggcggaga gctggcggaa gttcgcacga cggctccggc
     2341 gcaggaggta caggaatgaa gagtccattc gggaatatgt ccaacatctt gaagcaagcc
     2401 caggccatgc aggaacagat ggccaaggtg caggagcagg ccgcgaccaa gaccgtttcc
     2461 gggactgcgg gtggcgggat tgtgacggtc acggtcaacg gggccatgga cctgctgagt
     2521 gtgaagatcg atccagaggt cgtgaaggcc ggcgatgtcg aaatgctgca ggatttggtc
     2581 gtggcggccg gcaacgatgc gctgaaaaaa tcccgtgaaa tgatggcgga agaaatgaag
     2641 gctgtgacgg gcgggatgaa gatccccggt ttgttttgat ggcggcggcg tgcttcccgg
     2701 atgtgatgat gcgttatgag tgttgatcag cagggtctgt tggcgaagtt agtgcgggag
     2761 ttggtgcgtc tgcccgggat cggtcagaaa agcgcgcaac ggctggcctt tcatctgctc
     2821 aaggctgaac gggaagatgc gatgcgcctc gccgaggcga ttcaggcggt gaaggacgga
     2881 ctgtcgttct gccgacaatg ccggaatatt gccgaggggg aactctgtga gttctgccgg
     2941 gatcccaagc gggatcgcag caagatcctc gtcattgaag agcccagcac gttgtacgcc
     3001 atcgaacggg ccgggggcta tcgaggcctg tatcacgtcc tgctcggagt gttgtctccg
     3061 ttggatggcg tcggaccatc ggacattcgt gccgaggagt tgctggatcg agtgaaagcg
     3121 ggcggagtgg aagaggtcat cgtggcgacg aatcccacga tcgagggtga agcgaccgct
     3181 atctacttga cccggctctt gaagccgcat catgttcggg tgacccgcat cgcctatggc
     3241 attcctgtcg ggatggacat cgagtatgcg gacgaagtga ccctcgtcaa gtcgatcgag
     3301 ggccggaggg atctctgacc gggattttcc ggctcgggca tggtgatgcc tcggcgaagc
     3361 ctcgaagaac cgattgaccc ctttgatttc cggctgctat agtcaaattt ctgtatacgg
     3421 tctgaagaca ataccggcat ctcgattccg agtattcgat gaagactccc gcgaaaaaaa
     3481 ccacggtagc tcggcgaaag ccacccaagg caaacggggt caagtatccc gatatcctgg
     3541 cggaccttga aggccagcgt gcggcgattt tggctgaagc cggcgtggtg ttgacgaatc
     3601 cgaccggttt ggaagtattc cccgatgtga gtgatcaggc ttccgctgag gccgaccagc
     3661 atttctcgtt tcgtattcgg gagcgagagc gaaaactgct caagaaaatc gatgaggcgc
     3721 tggggcgatt tgccacgcaa acctacggca tatgcgaggg ctgtgaaggc gatattccct
     3781 acaagcgtct gaaagcccgc cctgtgacca cgttatgcat tgagtgtaag accagtcagg
     3841 aagaagccga aaagtccccc cgctgatctg tccccacgtt tctcacgttc ggctccatgc
     3901 cgggcgagtg ttcactctgc aatccaaata ggcctactgg tgcttgagtg cttgcacgcg
     3961 tttttgtgcg agcgcggcac ggtccgtttc ttcgagaatg ccttcgacga gagccaggta
     4021 ggtttcgtat tccgtaatgg cccgtcgcgg gttgtcatct gcgatggcct cggccacgtt
     4081 gaagcgggca atggcgtagt tagggcgcat caatatggct ttttcgaagg aggccagggc
     4141 atttttcttc tcgcccagct tccagtacac ggcaccaagg ttgttgacga cttccgggga
     4201 attggggcgt agggctaagg aggcgaggag ttcgctttta gcctt
//
LOCUS       DAOQRM010000199         4185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_22877_length_4185_cov_13.315981, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000199 DAOQRM010000000
VERSION     DAOQRM010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22877_length_4185_cov_13.315981"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..983)
                     /locus_tag="PKK81_12080"
     CDS             complement(132..983)
                     /locus_tag="PKK81_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33669.1"
                     /translation="MMPWTEPALGLEKYGRPLPSMDEPEHEAREEEETLFGGYLLTGA
                     FVKNPTFAARPDNTGLVGMRHMLHLETDLYKQYLTFYTDQNFFSDRTDGWIKLTEWDG
                     TFAFTGVLDRFNWRLQYERDAPLDRRGLKQAYADALVTARFQAIQDSTWWRRTFPNQN
                     LTAYAGAGWLFHNSQYFARPNNTGRALFRYVAHADLDVYKNKVVLYGDMNLFTDREAS
                     NTLNPSELDWIIGIAVRWKDTELAFYREEDRPLDQSGLVQKYYAVQLRFAFDVSKGAL
                     ERMGLKR"
     gene            complement(1049..1447)
                     /locus_tag="PKK81_12085"
     CDS             complement(1049..1447)
                     /locus_tag="PKK81_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33670.1"
                     /translation="MRLPHIRAHSGMLIPVLAGIMLGCHSTPPITEGYPHQRALQGKS
                     KEQVLACAGTPLQEREDGAVTMLRYYKEAPLLEESMVSSKSSRPTVHHGCWATVVIQH
                     QRVDQVQYRFVPSSVDASNDCEEIFANCPE"
     gene            2145..>4185
                     /locus_tag="PKK81_12090"
     CDS             2145..>4185
                     /locus_tag="PKK81_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV33671.1"
                     /translation="MSKHIAARNESVSVIGLLGQESRRQSSVPDRKKTSDAWSLSVAE
                     RRASRRLESRISTLLHFQDHTWNGQTKSLSLGGLSMEFSEDIPAMLNQQILLSVTPGA
                     TRPESVGVVCGIRVSESVLGSGQTKRGVTLAVQFARLSPGDEQVVAMWMSEGRARSKG
                     LHLVAALIAQESEDALIDTESRQPVVAQAVRARLARVPERTRQERRRQQRITVGLSTE
                     VSLRLRAGDRILSGALTTDLTSNGACLCLSAEEDLLGSELELKWVSSTVAQRGSSVST
                     PSVGCSVVGEVVWTRPGEAGAPSGYRQVGGRTVLAGVRFLPVAKEAEDIIEHLLGQQQ
                     GEGVVDRAEGPSVISEFSECVRPSGHRIALCHDRPRTDLSDDAPIVVLAPGYGESKRD
                     YVPLAYYLAGNGFHVVRFDNVNHVGESDGLVTQFRLEDMETDLETVLDYVAAQWPGRS
                     IGLVATSLAGRVALKVAGRVPHLGLLAMINGIMDVQHTLQAVHQEDLIGEHLAGVRKG
                     VVNILGLTIDADRWLEHAVQGGYADLHTTQQDAEHLHAPVVLFHAEQDAWVDPASIEA
                     VGAAMGANLRHSYVVPGALHRLQESPRKARTVYRQIALCCQQELWPTRSMARMVEPSH
                     REIGVQNRAERERSKKRRPVGKSDHVAFWQEYLQNFQTIPNVADFWRLMDHVYRLM"
BASE COUNT          768 a         1145 c         1347 g          925 t
ORIGIN      
        1 ctcttcgctt actgcggcct tgctggacgg cctttttgcg catcctgcgg gttagtgcaa
       61 taccgacagg gcgcatgagt tgatcgtcgt gtattgcgca acaatcgagt tttttcgcag
      121 cctgctaacg cctaccgctt cagtcccatc cgttccagcg cgcctttcga cacatcgaac
      181 gcgaagcgca attggactgc gtaatacttt tggaccaagc cggactggtc cagcggtcgg
      241 tcttcttcgc ggtagaaggc caattccgta tccttccatc ggacggcgat gccgatgatc
      301 caatcgagtt cggaagggtt cagggtgttg ctcgcttcgc ggtcggtgaa gagattcata
      361 tccccgtaca ggacgacctt gttcttgtag acgtccaggt cggcatgggc gacgtaccgg
      421 aacaatgcgc ggcccgtatt gttggggcgg gcgaagtatt ggctgttgtg gaagagccat
      481 cccgcgccgg cgtaggccgt gagattctgg ttcgggaagg tgcgtcgcca ccaggtggag
      541 tcctgaatcg cttgaaaccg ggctgtcacg agcgcatcgg cgtaggcctg tttcaggccg
      601 cgacggtcca gcggggcatc tcgttcgtac tgcagccgcc aattgaagcg atcgagcacg
      661 ccggtgaagg cgaaggtgcc gtcccattcc gtcaacttga tccatccgtc ggtgcggtcc
      721 gagaaaaaat tctgatcggt gtaaaacgtg aggtactgct tatagaggtc cgtttccaag
      781 tgcagcatgt ggcgcatgcc gacgagtccg gtattgtcag gcctggcggc gaacgtcgga
      841 ttctttacaa aggcaccggt taatagatag ccgccgaaca gcgtttcctc ctcctcgcgc
      901 gcttcatgtt ccggttcatc catggagggg agcgggcgcc cgtacttttc cagcccgagc
      961 gccggttccg tccagggcat cattgccgac agcaagccca tgaagaacag cagccagcgc
     1021 gggccaacac tgcggtgtcg ccgggccgtc attccgggca attggcgaag atctcttcgc
     1081 agtcgtttga ggcatcgacc gaactcggca cgaagcgata ctgcacctgg tcgactcgtt
     1141 ggtgctgaat cacgaccgtt gcccagcagc cgtgatgtac ggtaggacgg ctgctcttcg
     1201 aggacaccat ggactcttcg agcagcgggg cctctttgta gtaccggagc atagtaaccg
     1261 ctccatcctc tcgttcctgg aggggtgtgc cggcgcaggc cagcacctgt tctttacttt
     1321 ttccttgcag cgcccgctga tgaggatagc cctcggtgat aggcggggtg ctgtggcagc
     1381 ccagcatgat tccggccagg acggggatca gcatgccgct gtgagcacga atatgaggta
     1441 ggcgcatcgt gagtactcag gctgacctat cggggactgt gagtagcata cctgccgggt
     1501 caaagcaacc aacccgggcc gattccccga tgcctgcctt gtcgtcgaac cagcttgcgc
     1561 cgggccacga tgtcggtcac tgaggagggt cggcattaac cgtcatcggt gagccggtgc
     1621 cccgtggaga aggcaaattc tgccgcgtga aattccttaa tggctcgggt tctgatgcgg
     1681 cgatgagcgc cttgcagggt gtgcgtcagg cctttgcgcg aagtgaggaa tcggcgtcag
     1741 gatcatgacg gtcgtcatcg ttctgacttg ctggggctgc ttccgcgtcg tggcgacccg
     1801 gccaacggtg tctttcttcc ctgtagctat catctaagtt aatttgtgta ggtaactaca
     1861 ttttttttct gcggatgagt tgacaattat gtctaattcc ctagaataag cttcgaacat
     1921 tgggttgtat gacagtcgct gtgtgccaat tctgaggccg tgcgtgtcgt taatcctgcc
     1981 cttgttagat cagccgacat cctcccgcat ttttggaatc gttcgtgcgc ttgcaagagt
     2041 ccagcctcgc ccggagcgag ctctgtgagc gggcgacgac gtccacacgt tattcaattc
     2101 gatgctgtgc aacgcgcagc actcaatacg gcgggaggga atgtatgtcg aagcacatcg
     2161 ctgcgcgcaa tgagagtgtg tctgtgatcg gcctgcttgg ccaggagagc aggcggcaat
     2221 cttcggtgcc cgatcggaag aaaaccagcg atgcctggtc tctctctgtt gcagagcggc
     2281 gggcttcccg acgcctcgag agtcgcatct cgaccctcct tcactttcag gatcatacgt
     2341 ggaacggtca gacgaaaagc ctcagcttag gcgggctctc gatggaattt tccgaggaca
     2401 ttcctgccat gctgaatcag cagattctgc tgagtgtgac tcccggggcc acgcggcccg
     2461 aaagtgttgg ggttgtgtgc gggattcgcg tttccgaaag tgtccttggt tcaggtcaga
     2521 cgaaaagagg ggtgacactt gccgttcagt ttgcgcggtt gagccctggc gatgagcagg
     2581 tagtagccat gtggatgagc gagggccgcg cacggtcgaa ggggctgcat ctcgtcgccg
     2641 cattgatcgc ccaggagagt gaggatgccc ttatcgacac cgagtcccgg cagccggtcg
     2701 tcgctcaggc cgtgcgcgcg cgccttgccc gggtacccga gcgtacacgt caggagcgga
     2761 ggcgtcaaca gcggattacg gtcggattga gcaccgaggt cagtctccgg cttagagccg
     2821 gtgatcgaat tttgtcgggg gcgttgacga cagatttgac gtcaaacggg gcctgtctgt
     2881 gtctgtcggc cgaagaggat ttgctgggtt cagaattgga actgaaatgg gtgtcatcga
     2941 ctgtcgccca gcgaggatct tctgtgtcca cgccgtctgt cggctgctcc gtggtgggtg
     3001 aagtggtatg gacaagaccg ggagaggccg gcgctccttc cggctatcgg caggttgggg
     3061 ggcggacggt gttggcgggg gtgcgctttc tcccggttgc aaaagaggct gaggacatca
     3121 ttgagcacct gctcgggcag cagcagggcg agggcgtcgt cgacagggcg gaagggcctt
     3181 ccgtcatcag tgagttctcg gagtgtgtcc gcccgtccgg tcaccgtatc gcgctctgtc
     3241 acgatcggcc tcgaaccgat ctgtcggacg acgcaccgat tgtggtgttg gctcccgggt
     3301 atggggaaag caagcgcgac tatgtgccgt tggcctacta tcttgccggc aatgggtttc
     3361 atgtcgtgcg cttcgacaat gtgaatcatg tcggagagag cgatgggttg gtcacgcagt
     3421 ttcgcctgga ggatatggag accgatctgg agaccgtgct ggactacgtg gctgctcaat
     3481 ggccgggtcg atccatcggt cttgtggcga ccagcctggc cggtcgcgtc gcgctcaagg
     3541 tcgcggggcg agtccctcac ctggggctgc tggcgatgat caatggaatc atggatgttc
     3601 agcatacgct gcaggccgtc catcaagagg atctgatcgg cgaacatctg gccggtgtgc
     3661 gcaaaggggt ggtcaacatt cttggcctga ccatcgatgc ggatcgttgg ctggagcatg
     3721 ctgtgcaggg aggctatgcc gacctgcaca ccactcagca ggacgccgag cacctccatg
     3781 ctcccgtcgt gctgtttcat gccgagcagg atgcgtgggt cgatccggcc tccatcgagg
     3841 cagtcggcgc ggcgatgggt gcgaatctcc gccactccta tgtggtgccg ggcgcgttgc
     3901 atcgacttca agagagcccc cggaaagccc gcacggtcta tcgccaaatt gccctatgct
     3961 gccagcagga gttgtggccg acacggtcga tggcgcgcat ggtcgaacca tcgcatcgag
     4021 aaatcggcgt gcagaaccgc gcggaacgcg aacgcagcaa gaagcgtcgg ccggtgggca
     4081 agtcggatca tgtggcgttc tggcaggagt acttacagaa tttccagacg atcccgaacg
     4141 tggccgattt ttggcggctc atggatcatg tctatcgcct gatgg
//
LOCUS       DAOQRM010000200         4157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_23111_length_4157_cov_19.127011, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000200 DAOQRM010000000
VERSION     DAOQRM010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23111_length_4157_cov_19.127011"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKK81_12095"
     CDS             <1..279
                     /locus_tag="PKK81_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33672.1"
                     /translation="DSSATTEQSCSFGMANGDPTHQCQVPIPAGCVIARFPGTDKPWT
                     TISKAGRTFCTFDQKGTDWKTRITGQCTRCDSGHCTGQFMVRFECAKP"
     gene            279..1310
                     /gene="queG"
                     /locus_tag="PKK81_12100"
     CDS             279..1310
                     /gene="queG"
                     /locus_tag="PKK81_12100"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNV33673.1"
                     /translation="MSASLTVAIKQAARELGFDAVGISLLPTQTNPAVNLNREASAGD
                     LLDRLQEWLRRGYHATMAWMVRDPERRADPTRVLPGCRSIISVGMNYYTDYRADERQG
                     NGRIARYAWGLDYHKILGDKLAQLAERIGALAPASLNRAYVDTGPVMEKVWAQQAGLG
                     WIGKHSNLVSTDFGSWLLLGEILTTLELEPDEAGTDLCGSCTLCIQACPTGAITEPYV
                     VDAGRCISYLTIELRGTEPPIAEDLRRELGNRIFGCDDCLDICPYNVQARPTTEPGFQ
                     PSPLTGAPSLLALTKMDEQTFATTFKHSPIRRAKYAGLQRNLSWALSNETAPIDRHST
                     TPPPSADPR"
     gene            1404..2789
                     /locus_tag="PKK81_12105"
     CDS             1404..2789
                     /locus_tag="PKK81_12105"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV33674.1"
                     /translation="MHAPTLLIVEDEERMRRLFELVLKPAGYQLLLARSGDEALRVIQ
                     EHESLDMIITDLQLGAVSGMDVLEAARQQVPDVPVLIVTGYGTVKSAVEAMQKGAYDY
                     ISKPVDNEELKIVIARALQVRQLARDNRILRAGLHEQFGFDRIVSVSKEMELIKRLAR
                     EVAQTDATVLITGESGTGKDLLARAIHLVSPRAQGPMVALNCAGIPEHLLESELFGYE
                     KGAFTDAKKSKPGRFQMADRGTLFLDEIGELSLTAQAKLLRVLEQHVVEPLGGVRSVA
                     VDIRVIAATNQELPDLIKAGRFRLDLYYRLNVYQLRMPPLRERPEDIEPILTQFLGQA
                     RRERGSRIKGMTAEALTILKQYPWPGNVRELHNVVEWLTITCKQDEITPEHLPASFKT
                     APPTNEEKPAGTPSLLALGLSVEEVEKTMLQEALRKTGGNVSEASRLLKITRNTLRYR
                     MAKHNLSLPPG"
     gene            2789..>4157
                     /locus_tag="PKK81_12110"
     CDS             2789..>4157
                     /locus_tag="PKK81_12110"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV33675.1"
                     /translation="MRRLRRTGGLQRKFFVALLIVGIVPGMVALWATYRSSTATLKQA
                     IGEGFQEIARSTSIRLATAVDNEIDRAIRLALVPLHVRQPVVLANHQADATADKTQHQ
                     PSPAHPKSNNPPAVDQDTTSYLHEWAGESRHYVRVTIADRQGQVVASTDPRLPPQQAG
                     EVWWREAIQAAPGTSYVSNVTFDPQVNDMVFHVAVPIVDDTRQATIGVVDLLIRRNLL
                     TQMILPIQIGNTGHAMLLDTQGTPLICPVLPPTAHLIPSALMNRLTLDRPLWLVAEND
                     AHGGRDAIVGAAPVRLTHPLTPGSLDGNRWYAFIRQAPEETYAPIYSLLLTVGVIGFG
                     LVIVLSSFGFLVGYRIVTPIVALQREAEGLRLTLALPENGSANRPRTPLLSPGYQTGD
                     EIEDLATSFAAMREVLEENLRTIRSQQHELIRQEKLASVGQLLAALAHDLRNPLGVIR
                     SSAQVV"
BASE COUNT          849 a         1423 c         1174 g          711 t
ORIGIN      
        1 gactcctccg cgacgaccga acaatcctgc agtttcggca tggcgaacgg cgatcccact
       61 caccagtgcc aggtgccgat tcccgccggc tgcgtcatcg cccgcttccc gggaaccgac
      121 aaaccgtgga cgaccatctc caaagccggt cgcacgtttt gcaccttcga tcagaaaggc
      181 acggactgga agacgcgcat taccggccaa tgtactcgct gcgattccgg ccattgcacc
      241 gggcaattca tggtgcgatt cgagtgcgcg aagccgtaat gtccgcgtcc ctcacagtcg
      301 ccatcaaaca agccgcccgc gaactcggct tcgacgccgt cggtatcagc ctccttccta
      361 cgcaaacgaa cccagccgta aatctgaatc gtgaggcctc ggcaggtgac ctgctggacc
      421 ggctccaaga atggctcagg cgcggatacc acgcgaccat ggcctggatg gtccgtgatc
      481 ccgagcggcg agccgatccc acccgagtac tgcccggctg ccgatcgatc atttctgtcg
      541 gcatgaacta ttacacagac tatcgggccg acgaacgcca gggaaacggg cgcattgccc
      601 gctatgcctg gggcctcgac taccacaaga tccttggtga caagttggcc caactcgccg
      661 agcgcatcgg cgcgctggcc ccggccagcc tcaaccgggc ctatgtcgac acggggccgg
      721 tcatggagaa ggtctgggcc cagcaggccg gactcggatg gatcggcaaa cactcgaacc
      781 tcgtctcgac agacttcggc tcctggctgc tcctgggaga aattctgacg accctggagc
      841 tggagccgga tgaggcggga accgatctct gcggaagctg cacgctctgc atccaggcct
      901 gtccgaccgg cgcgatcacc gagccatacg tcgtcgatgc cggacgctgc atctcctacc
      961 tgaccatcga acttcgcggc accgaaccgc cgatcgccga ggacctcagg cgagagctgg
     1021 gcaaccggat tttcggctgc gacgattgcc tggacatctg cccctacaac gtccaggcca
     1081 gaccaaccac cgaacccggc ttccaacctt ccccgctcac cggtgccccg tcgctgctgg
     1141 ctctgacgaa gatggacgag cagaccttcg ccaccacgtt caaacacagt cccatccgcc
     1201 gcgcaaaata cgccggactc caacgcaatc tctcctgggc gctctccaat gaaaccgccc
     1261 ccatcgatcg gcacagcacc acacctccgc cgagcgccga tccccgctaa gccccaccca
     1321 ctgtttctcc ccggccgata gtggtaggct acggtctcat gtcctccctc atgtgaggac
     1381 ccacctcagc cggttgcgag gccatgcacg cacccaccct cctgatcgtc gaagatgaag
     1441 aacgaatgcg ccggctcttc gagctggtgc tgaaacctgc cgggtatcag ctgctcctgg
     1501 cccgctcggg ggacgaagcc ctccgcgtga tccaggaaca cgaatccctc gacatgatca
     1561 tcacggatct ccaattgggc gccgtctcgg gcatggatgt gctggaagca gcccggcaac
     1621 aggtgcccga tgtgccggtc cttatcgtga ccggatacgg caccgtcaaa tcggccgtcg
     1681 aggccatgca gaaaggcgcc tacgactaca tctccaagcc cgtcgacaat gaagagttga
     1741 agatcgtcat tgcccgcgcg ctgcaggtcc gccagctcgc ccgggacaat cgcatcctgc
     1801 gcgcagggtt gcacgaacag ttcggcttcg accgcattgt cagcgtctcc aaagagatgg
     1861 aacttataaa gcggctcgcc cgggaagtgg cgcagaccga cgccacggtg ctcatcacag
     1921 gcgaaagtgg aacggggaag gatctgctcg cgcgcgccat ccacctcgtc agcccacgcg
     1981 cccaaggccc gatggtggct ctgaactgcg ccggcatccc cgagcacctg ttggaatctg
     2041 aactgttcgg gtatgaaaag ggagcgttta ccgacgcgaa gaaatccaaa ccgggacgct
     2101 ttcaaatggc ggatcgaggc acgctgttcc tggacgaaat cggcgaactg agtttgacgg
     2161 cacaggccaa actcctgcgc gtcctggaac agcatgtcgt ggaaccgttg ggcggagtgc
     2221 gcagtgtcgc cgtcgatatt cgggtcatcg ccgcgaccaa ccaggaatta cccgatctca
     2281 tcaaagccgg ccgtttccgc ctcgatctct actatcgcct gaacgtctat caactccgga
     2341 tgccgcccct tcgcgaacgg ccggaggaca tcgagccgat tctgactcag ttcctcggcc
     2401 aggcccgtcg ggaacgcggc agccgcatca agggaatgac cgccgaggcg ctcacgattc
     2461 tcaaacagta tccctggccg ggcaacgtgc gagaactgca caatgtcgtg gagtggctga
     2521 ccatcacctg caaacaggac gagattacgc ccgagcacct gccggcctcg ttcaagaccg
     2581 ctccgccgac aaacgaagag aagccggccg gcacaccgtc gctcctcgcg ctgggactct
     2641 ccgttgagga agtcgaaaag acgatgctcc aggaggcgtt gcgcaaaacc ggggggaacg
     2701 tatccgaagc cagccgtctg ctcaaaatca cacgcaacac gttgcgatat cgaatggcca
     2761 aacacaacct gtcgctgccc ccaggctgat gcgtcgccta cgacgaaccg gcggactcca
     2821 gagaaaattc ttcgtcgcgc tgctcattgt cggcatcgtg cccggcatgg tcgcgctctg
     2881 ggcgacctac cgctccagca ccgccaccct caagcaagcc atcggtgaag ggttccagga
     2941 gatcgcgcgt tccacctcca ttcgactggc gaccgccgtc gacaatgaaa tcgatcgcgc
     3001 catccggctg gcgctcgtcc cgctgcacgt ccgtcagccg gtcgtcctcg ccaaccacca
     3061 ggccgatgcc acagccgaca agacacaaca tcagccatca ccggctcatc cgaaatccaa
     3121 caacccgccc gcggtggacc aggacaccac aagctacctc cacgaatggg cgggcgaatc
     3181 ccggcactat gtgcgtgtca ccattgccga ccgacagggt caggtcgtcg cctcgacaga
     3241 tccgcggcta ccgccgcaac aggcgggcga agtctggtgg cgtgaagcca tacaggccgc
     3301 acccggcaca tcctacgtca gcaacgtgac gttcgatcct caggtgaacg acatggtctt
     3361 ccatgttgcc gtcccgatcg ttgacgacac ccgccaggcc accatcggtg tcgtcgacct
     3421 cctcatccgt cgcaatctgc tgactcaaat gatcctcccc atccagatcg ggaacacggg
     3481 tcatgccatg ctgctcgaca cgcaaggcac accgttgatc tgcccggttc tgccgcccac
     3541 cgcccatttg attccatcgg ccttgatgaa ccgactgacg ttggatcgcc ccctgtggct
     3601 ggtcgcagag aatgatgcac acggcggccg cgatgcgatc gtcggggcgg cgccggtccg
     3661 gctgactcat ccactgacgc cggggagcct cgacggcaac cgatggtatg cgtttatccg
     3721 gcaggcgccc gaggaaacct atgcgccgat ctattccctg ctgctgacgg tcggcgtaat
     3781 cgggttcggg ctggtgatcg tgctctcctc cttcgggttc cttgtcgggt accgcatcgt
     3841 gacaccgatc gtggcgctcc agcgcgaagc ggaaggcctt cgcctcacac tggccttacc
     3901 ggagaacggc agcgcgaacc gaccccgtac cccattgctt tcgccgggct atcagaccgg
     3961 tgatgagatt gaagatctgg ccacgagttt cgccgccatg cgggaagtgc tggaggaaaa
     4021 tctgcggacg atccggtcac agcaacatga gctgatccgg caggaaaagc tggcgtctgt
     4081 gggacaactg ctggccgccc tcgcccatga tctgcggaac ccgctcggcg tcatccggag
     4141 ttccgcacaa gtcgtgc
//
LOCUS       DAOQRM010000201         4140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_23272_length_4140_cov_12.281518, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000201 DAOQRM010000000
VERSION     DAOQRM010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23272_length_4140_cov_12.281518"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKK81_12115"
     CDS             complement(<1..419)
                     /locus_tag="PKK81_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV33676.1"
                     /translation="MASIAQIMNKKPQSIGPTTSIRGAAKKMRDLRVGSLLIKKGKNF
                     VGIVTDTDLVRKGLATSQDVSKLTVEKIMTTPLCTIESNQAVDDAQDMMGDLGVRHLA
                     VTKGGSIVGVVSVRDLLMHYKRYAQSRLADKQVYSEP"
     gene            complement(470..1501)
                     /locus_tag="PKK81_12120"
     CDS             complement(470..1501)
                     /locus_tag="PKK81_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNV33677.1"
                     /translation="MNAVVFHEHGGPGKLQYQEMPMPTIGVDEVLVRVKACALNHLDI
                     WIRQGSPAYPMPLPHILGSDISGVVHQIGSHVEGVAEGERVYVSPGVGCGRCEHCWAG
                     RDNMCRSYGLIGAMCHGGYAEYVKVPARNVFPVPGALSFEQAAAFPLVSVTAWHMLFG
                     LAAVQPGEDVLIMGAGSGVGHMAIQMAKLAGARVLTTVGSDDKIAKAKTLGADEVINH
                     TREQVNQRVRELTHRRGVDVVIEHIGPEVWTQCIDALAKGGRLITCGATTGAEVKLDL
                     RYVYSRQLTIRGSYMGSQSELLKAAQLVGQGRLQPVIDRTFPLSEAKAAQEYLLNRKF
                     FGKIVLSVS"
     gene            complement(1790..>2199)
                     /locus_tag="PKK81_12125"
     CDS             complement(1790..>2199)
                     /locus_tag="PKK81_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HNV33678.1"
                     /translation="PVIIVPVRTLTAVRQIIPDVAAIADLSQQYGANGIMVFSTMTVE
                     QSSSVHTRMFAPLIGIVEDPATGSASGALGAYLVQHGVVDIRPSTEITAEQGYEIDRP
                     SRILIQVDSDDDVIQGVTVGGQAMMVVEGTLTF"
     assembly_gap    2200..2299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2300..2400)
                     /locus_tag="PKK81_12130"
     CDS             complement(<2300..2400)
                     /locus_tag="PKK81_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNV33679.1"
                     /translation="MPGPRRLQFYQADVFTDQPFGGNPVAVFPDADGL"
     gene            complement(2629..3870)
                     /locus_tag="PKK81_12135"
     CDS             complement(2629..3870)
                     /locus_tag="PKK81_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33680.1"
                     /translation="MATALPIFQPTPIFKDRTDYRQVLIKEMEAGKIPLSLGRDCPVK
                     CEFCYELDHSYRETLDPPKTSDDDWKFILNYISKKPTDPTQFWCLGGNEFMEWTDLFL
                     HPKAMEWVEDFLRETDKSIQFFTVGFVHVPKIHQLVAQYPGRINFELSVITLSDYRQR
                     LMPHAPSVKHLMKVLDGPAVSSANFYAFDKDTMSKDAATISKVNRQCVLWMGTLTPVR
                     GLKEETAALMRQGRTFLPEEASHVYDLALPNLQTIHTEAYTTAFLSRRRIVSLFDSLE
                     LEKKDTVVMAGSVHKILTMYRKHRARFLYVPNALLSGDSDCTVLLTFDDIARRLTKEK
                     VVHVPKCIMQSGRGPYTDITGVTLEQFSKKTGVRVKVLHKIDTRFANQQLYRNGSLQN
                     YVEQYLKNPLRGAYEAMPRPV"
     gene            3953..>4140
                     /locus_tag="PKK81_12140"
     CDS             3953..>4140
                     /locus_tag="PKK81_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33681.1"
                     /translation="MTEELLTKALELFTLTQPYTREQLDQKREELLHTWTPRRFANLT
                     NNPKKYMQAYTKAEEMTR"
BASE COUNT          841 a         1283 c         1110 g          806 t
ORIGIN      
        1 ttcggttcag agtacacctg cttatccgcc agtctcgatt gcgcataccg cttgtagtgc
       61 atcaagagat cgcgcaccga caccacgcca acgatcgaac cgcccttggt caccgcaaga
      121 tggcgcacac cgagatcgcc catcatatcc tgcgcgtcat cgaccgcctg attgctctca
      181 atggtgcaga gcggagtggt catgatcttt tccaccgtga gtttcgatac gtcctggctg
      241 gtcgccagac ccttgcggac gagatccgta tcggtcacga tccccacaaa gttcttcccc
      301 ttcttaatca ggagtgaacc gacccgcaag tcacgcatct tcttcgccgc accgcgaatc
      361 gacgtcgtcg gaccaatgct ctggggtttc ttgttcatga tctgcgcaat actcgccatg
      421 tcacatacct ttctgcccgt tgggacaaga gagccactaa tgaaaccggt tatgaaacgc
      481 tcagcacgat cttaccaaaa aattttcgat tcagaagata ttcttgcgcc gccttcgctt
      541 cggagagtgg aaacgtccgg tcgatcacag gctgcaacct gccttggccg accaactgag
      601 cagccttgag caattcactc tgcgacccca tgtacgagcc ccggatcgta agctgccgtg
      661 aatagacata ccggagatcg agcttcacct ccgcgcccgt cgtggcgccg caggtaatca
      721 accggccgcc tttggccaat gcatcgatac actgtgtcca cacctccggg ccgatgtgtt
      781 cgatcactac atccacgccg cggcggtgcg tcaactcgcg cacccgctgg ttcacctgct
      841 cgcgggtatg attgatgacc tcgtcggcgc cgagggtctt ggccttcgca atcttgtcat
      901 cgctcccgac ggtggtcagc acacgggcac cagccagctt ggccatctgg attgccatgt
      961 gccccacccc gctgcccgcc cccatgatca agacgtcttc accgggctgc accgcagcca
     1021 acccgaacaa catatgccaa gcggtcacag acaccagcgg aaacgccgcc gcctgctcga
     1081 aactcaacgc gccgggaacc gggaacacat tccgcgccgg caccttcacg tactcggcat
     1141 atccgccatg gcacatcgcc ccgatcaacc cgtaggaccg gcacatgttg tcgcgaccgg
     1201 cccaacagtg ctcgcagcga ccgcagccga cgccgggcga cacatacacg cgctcgccct
     1261 cggcaacccc ttccacgtgc gagccgatct ggtgcaccac gccggagatg tcggacccga
     1321 ggatatgcgg aagcggcatc ggataggcgg gactgccctg ccggatccag atgtcgagat
     1381 ggttcaacgc gcaggctttg acccgcacga gcacttcatc gacaccgatg gtcggcatcg
     1441 gcatctcttg atactgcagc ttccccggcc cgccatgttc gtggaacacc actgcgttca
     1501 tatcactcct cactcgcgag tcgcggctgt attgcgtttc cgcctcaatg cgctctttcc
     1561 gcggaggata gcgagataca gctttccagc aagatgatca aaactccatc tgacaaggcc
     1621 gcagggagtg cgccgactga ggacgtacat accaaacttg tttgacccgt tcgccaacca
     1681 gatgctcttg gcgaacggat aaaccccttc agcgcgccct cttatactgc taacgtcaca
     1741 aggagacgta cgactgagaa cgcagtgaga tggcgttttc atcatccggc taaaaggtga
     1801 gcgtcccctc caccaccatc atggcctgtc cgccgaccgt cacgccttga atgacatcgt
     1861 cgtctgagtc cacctggatc agaatgcggg aggggcgatc gatctcgtac ccctgctcgg
     1921 cggtaatctc ggtcgatggc cggatgtcca ccacgccatg ctggaccaga taggcaccca
     1981 acgccccgct cgcacttccg gtcgcgggat cttccacgat gccgatcaac ggggcgaaca
     2041 tccgcgtatg cacgctggag gactgttcga cggtcatcgt gctgaacacc atgatgccgt
     2101 tggcgccata ctgctgggac aggtccgcga tggccgcgac gtcgggaatg atctgccgca
     2161 ccgccgtaag cgtccgcacc ggcacgatga tgaccggcan nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnna ggccatcggc atcgggaaac acggcgacgg gattcccgcc
     2341 gaacggctga tccgtgaaca cgtcggcttg atagaactgc aatcgcctgg gaccgggcat
     2401 agattcctca cgaaagatgt tggcggcatg ttgccccgag cgggtatttt ttgcaagagg
     2461 gcgactgagc gaagcaggtg agcgagtagg aatttggcgg ctggtcaaaa cgctatgtgc
     2521 ctaggccgca gggagcatgg cgactgaggc gtacccttgc ggtacatcgc agggagacat
     2581 gcgaccgaga acgacgggag atggcgtttt caccagccgg ctaccctgct aaaccgggcg
     2641 cggcatagcc tcatacgcac ccctcaacgg attcttgagg tattgctcga catagttctg
     2701 aagcgatccg ttgcggtaga gctgctggtt cgcgaagcgg gtatcgatct tgtgcaacac
     2761 cttcacccgc acgccggtct tcttgctgaa ctgctccagc gtcacgccgg tgatgtcggt
     2821 atagggcccc cgccccgact gcatgatgca cttgggcaca tggaccacct tctccttagt
     2881 cagacgtcgc gcaatgtcgt cgaaggtcag gagcaccgtg cagtcggaat cgccgctcag
     2941 caaggcgttt ggcacataga gaaaccgggc gcggtgcttc cggtacatgg tcaggatttt
     3001 gtggacgctg cccgccatca cgaccgtgtc tttcttctcc aactccagcg agtcgaacag
     3061 gctgacgata cgccggcgac tcaagaaggc ggtggtgtac gcctccgtgt ggatcgtttg
     3121 gagattgggc agcgccagat catagacgtg gctggcttcc tccggcagga atgttctccc
     3181 ctgccgcatc agcgccgccg tctcctcctt gagcccgcgc accggagtga gcgtccccat
     3241 ccacagcaca cactgccgat tcaccttgga gatcgtcgcc gcatccttcg acatcgtgtc
     3301 cttgtcgaac gcgtaaaaat tcgccgacga cacggcagga ccgtccagca ccttcatgag
     3361 atgtttcacg gaaggcgcat gcggcatcaa ccgctgccgg tagtcgctca gtgtgatgac
     3421 cgacagttcg aagttgatcc ggccaggata ctgcgccacc agctgatgga tcttcggcac
     3481 atggacgaac ccgaccgtga aaaactgaat gctcttgtcc gtctcgcgga ggaaatcctc
     3541 cacccattcc atcgccttgg gatgcaggaa caggtcggtc cattccatga actcgttgcc
     3601 gcccaggcac cagaattgtg tggggtcggt gggtttcttg ctgatgtagt tgaggatgaa
     3661 cttccagtcg tcgtcgctgg ttttcggcgg gtcgagcgtt tcgcggtagg agtggtcgag
     3721 ttcgtagcag aactcgcatt tgaccgggca gtcccggccc aggctcaggg gaattttccc
     3781 cgcctccatt tctttgatga gcacctgtcg atagtccgtg cgatccttga agatcggggt
     3841 gggctgaaaa atcgggagtg cagtggccat ggtggaacac cttgcctgtt gccgcgaggg
     3901 gcaacgcctg ctagattgac agcgtcatta tcgacttgcc agactccgtt acatgaccga
     3961 ggaactcctc accaaagccc tggaactttt caccctcacg caaccctata cccgcgaaca
     4021 actggatcag aagcgcgagg aacttctaca cacctggacg ccccgccgct tcgccaacct
     4081 gaccaacaat cccaagaaat acatgcaggc ctacacaaaa gccgaagaga tgacccggca
//
LOCUS       DAOQRM010000202         4054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_23968_length_4054_cov_16.014004, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000202 DAOQRM010000000
VERSION     DAOQRM010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23968_length_4054_cov_16.014004"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PKK81_12145"
     CDS             complement(<1..308)
                     /locus_tag="PKK81_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV33682.1"
                     /translation="MSTPDLNLLITLDVLLAEGSVARAATRLRLSPSAMSRALARLRE
                     TTGDPLLVRAGRGLVPTPRAVELRERVSQVVEEGQAVLRPAEKLNLTQLVRTFTLRTS
                     "
     gene            477..1115
                     /gene="tmpT"
                     /locus_tag="PKK81_12150"
     CDS             477..1115
                     /gene="tmpT"
                     /locus_tag="PKK81_12150"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HNV33683.1"
                     /translation="MEPQFWHDRWTKNEIGFHKSDANPLLVKYFTELALPKGSLVFVP
                     LCGKTLDISWLLSNGYRVAGAELSSNAIEQLFAELGVKPTITRNGEIDRYHANDIDIF
                     VGDIFDVTRTMLGPVDAIYDRAALVALPENMRSRYTAHLTAITDHAPQLLVCYEYDQR
                     AAEGPPFSISHEEVRRHYGERYHLTLLTSADVPGGLKGKCSATEHVWLLRRG"
     gene            1133..2584
                     /locus_tag="PKK81_12155"
     CDS             1133..2584
                     /locus_tag="PKK81_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV33684.1"
                     /translation="MPGRDVVQAGTTDLTPSIRWILAGLSLSMLLSSIGTSIANVSLP
                     TLAQAFDASFQQVQWVVIVYLVTITTVIVSVGRLGDMTGRRRLLLVGVFLFTVASVLC
                     GVAPTLWLLIAARAAQGLGAAIMMALTMAFVGETVPKSQVGSAMGLLGTMSAIGTALG
                     PSLGGMLISEFSWRAIFLINLPLGLLIFLLAYRFLPVDRPRQQVDRLGFDHAGTLLLA
                     LTLAAYTLAMTIGRGSFGPRNLALLVATLLGAGLFLFAETRAESPLIRLTMFRNPLLS
                     ASLIMSALVSTVMMATLVVGPFYLSRTLGLHAALIGLVLSVGPLCAALTGVPAGRLAD
                     RLGPQRMTVLGLIGIAAGAFLLSVIPATLGIYGYTAPLIVITVGYALFQTANNTAVMT
                     DTRPEQRGVISGLLNLSRNLGLITGASVMGAVFAAASSTIDITTAPPAAVAIGMQVTF
                     AVAAVLILVALAIAVATYRRELYGWAMAPTAES"
     gene            2823..>4054
                     /gene="pyk"
                     /locus_tag="PKK81_12160"
     CDS             2823..>4054
                     /gene="pyk"
                     /locus_tag="PKK81_12160"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNV33685.1"
                     /translation="MQKTRIICTIGPATESYEMLHKLYEAGMSIARLNMSHGDHESHA
                     KVIQHIKSLNRKVKFPIPILLDTQGPEIRTGDLSNELDLRQGDIVSVTTRGPMNVEES
                     SIHINYADLLEAVNVGDRITVDNGLINFEVLEKHERHMRCRVLDGGLLKSKRHVNLPG
                     VRVNLPSITQKDIKDILFGLERDVDFIALSFVREAGDIQQLKDLMGDKVGKVKIVAKI
                     EDQEGVRNLEAIIKESDGIMVARGDLGVEINLEDLPNVQRTIVRLCAEYGKRVIVATH
                     LLESMIHNPHPTRAEVTDVANAIYEEADAVMLSGETTVGKYPVKCVEFLRRIALKSET
                     IPGLQFAKHLRNAENKQQLAAAAVQLAEGVKAKGIVVITRRGLMADLVANCRPFATNI
                     YAFTNMSQPRRTMMLNRGV"
BASE COUNT          804 a         1226 c         1187 g          837 t
ORIGIN      
        1 tcgctggtcc gcaatgtgaa cgttcggacg agctgtgtga gattgagctt ctccgcaggg
       61 cgcagaaccg cttgtccctc ttccacgacc tgactgaccc gttcacgaag ttcgaccgct
      121 cgtggtgtgg ggacgagacc tcggccggcc ctcaccagga gtggatcacc ggtcgtctca
      181 cgcaatcgcg ccaacgcccg gctcatggcc gacggactca ggcgcagtcg tgtggcggcg
      241 cgagccacac tgccttccgc caataaaaca tccagggtga tcagcaaatt gagatcaggt
      301 gtggacatgc accgaccata ccatagcttg accgtgatgt ggcgtctcac gcacgaatac
      361 agtgcagatg atgcgccttc cgccatgtcg tttggaaggt tatacttccg acagttcgat
      421 tcgggatacg cgggaacaag gagtgcgccg gtcacttaac agagaggtca cggactatgg
      481 agccacaatt ttggcatgac aggtggacaa aaaatgagat cgggtttcac aagagtgacg
      541 ccaacccgct gctggtgaag tatttcaccg agctcgctct gccgaaaggc agcctggttt
      601 ttgtgcccct gtgcgggaag acgctggata tttcgtggct gctgtcgaac ggctaccggg
      661 ttgccggagc cgaattgagc tcaaacgcca ttgagcaact gttcgcggag ctcggggtga
      721 aaccgacgat cacgaggaac ggtgagatag atcgctacca tgcgaacgac atcgacatct
      781 ttgtcggcga tatcttcgat gtgacgagaa cgatgctggg tccggtcgat gcgatctatg
      841 acagggctgc gctggtcgcc cttccggaga acatgcggag tcgctacacc gcgcacctca
      901 cagcgatcac cgatcacgcg ccgcagttac tcgtttgtta tgaatacgat caacgcgcgg
      961 cggaaggccc ccctttctca atcagccacg aggaagtccg tcgacattat ggagagcgat
     1021 accacttaac gctcctcacc agcgcagatg tgcccggtgg actcaaggga aaatgttcgg
     1081 caaccgagca tgtgtggctg ctacgaaggg gctgaggatg atcacgcgat ttatgccagg
     1141 acgagatgtg gtacaagcag gaacgacgga tctgacgcct tcgattcggt ggatactcgc
     1201 cggcctgtcg ttatccatgt tgttgtcctc gatcgggacg agcatcgcca atgtgagctt
     1261 gccgacgttg gcacaggcgt tcgatgcctc gtttcaacag gtccagtggg tcgtcatcgt
     1321 gtatctcgtg accatcacca ccgtgatcgt cagcgtgggt cggctcggcg acatgaccgg
     1381 tcgccgacgg ctcctcctgg tcggtgtctt cctgttcacc gtcgcctcgg tcttgtgcgg
     1441 tgtcgctccc acgctctggc tgctgattgc cgctcgcgcg gcgcagggtc tgggagccgc
     1501 catcatgatg gccctcacta tggcattcgt cggcgagacg gttcccaagt cacaggtcgg
     1561 cagcgccatg ggactgcttg gcacgatgtc tgcgatcggc accgcgctcg gcccatcgct
     1621 cggcggcatg ctgatctccg aattcagctg gcgggcaatc ttcctgatca acctgccgct
     1681 agggctcctg atttttcttc tcgcgtatcg attcctaccc gttgatcgtc cgcgacagca
     1741 agttgatcgg ctcgggttcg accatgcggg tacgctgctg cttgcgctga cgcttgcagc
     1801 ttatacgctc gccatgacga ttggacgcgg cagcttcgga ccgcgcaacc tggcgctgct
     1861 ggtggccacc ctacttggag ccggcctctt cctgtttgcc gagacaaggg ctgaatcacc
     1921 tctgatccga ttgacgatgt tccgcaatcc tctgctgagc gcaagcctta tcatgagcgc
     1981 actcgtctcg acggtgatga tggcgacgct ggtggtcggg ccgttttatc tctcgcgtac
     2041 gctcgggctc cacgcggcac ttatcggact ggtgttgtcg gtgggccctc tttgcgccgc
     2101 gctgaccggt gtgccggccg gtcgccttgc ggaccgcctt ggtccccaac gcatgaccgt
     2161 ccttgggctc atcggaatag ccgccggtgc cttcctcctg tccgtaatac cggcgacgct
     2221 cgggatctat ggctacaccg cccccctcat tgtcatcacc gtcggttatg cactgttcca
     2281 gacagccaac aacactgctg tcatgacgga tactcgtccg gagcagcggg gcgtcatttc
     2341 cggcctgctc aacctgtccc gcaatctcgg gctgatcacc ggggcttccg tcatgggcgc
     2401 tgtgttcgcg gctgcgtcat cgacgatcga catcacaacg gcgcctcctg ctgccgttgc
     2461 catcggtatg caggtcacgt tcgcagtcgc ggcggttctg atcctcgtcg cgctcgccat
     2521 cgccgtggca acgtaccgcc gtgagttgta cgggtgggcg atggctccga ccgcagagtc
     2581 ataggcggac agcaggattc cggcagcacc gcttgccgct cgtcatccgc aagtgcctgt
     2641 ggcacaggtt gcgaaccatg ctgcacgaga catggattgc atccctttgc gccacgatgg
     2701 actcgaccgg cgagaccaag tagaatgacc ccgttcttct cgtcttcccc gcgcgacatc
     2761 gtcatcattt cgtcgggtgc agctcagcct gcaaggatcg tccattaccc caagggagct
     2821 acatgcaaaa gactcgcatc atctgcacga tcggtcccgc cacagaatcc tacgaaatgc
     2881 tgcacaagct ctacgaggcc gggatgagta tcgcccgtct caacatgtcc cacggcgacc
     2941 acgagtctca cgccaaggtc atccagcaca tcaagtcgct caaccggaag gtgaaatttc
     3001 ctattcccat tctcctggac acgcaaggtc cggagatccg caccggcgat ctttccaacg
     3061 agctcgacct gcggcaaggc gacattgtct ccgtcaccac acgcggtccg atgaacgtcg
     3121 aggaaagctc catccacatc aactatgcgg acctgcttga ggcggtcaac gtgggggacc
     3181 ggatcaccgt ggacaacggg ctgatcaatt ttgaggtgct ggagaagcac gaacgccaca
     3241 tgcggtgccg tgtcttagac ggcgggcttc tgaagagcaa acgccacgtg aatctccccg
     3301 gcgtccgtgt caatctcccg tcgattacgc aaaaagatat caaggatatt ctttttggtc
     3361 tggagcggga tgtggacttc atcgccctgt cgtttgtgcg cgaggccgga gatattcagc
     3421 agctgaagga tttgatgggc gacaaggtcg ggaaggtaaa gatcgtcgcg aagatcgagg
     3481 accaggaagg agtccggaac ctggaagcga tcatcaagga atcggacggc attatggtcg
     3541 cgcgcggaga tctcggcgtg gagatcaacc tggaagacct gccgaatgtc cagcgcacca
     3601 tcgtgcgact ctgtgccgag tacggcaaac gcgtgatcgt ggcgacgcat ctcctcgaat
     3661 ccatgatcca caacccgcat ccgacccggg ccgaggtcac cgacgtcgcc aacgccatct
     3721 acgaagaagc cgatgccgtc atgctgtccg gcgaaaccac cgttgggaag tatccggtga
     3781 agtgcgtcga gttcctccgc aggattgcgc tgaaatccga aacgataccc ggattgcagt
     3841 ttgcgaaaca tttgcgtaac gcggagaaca agcaacaact ggcggcggcg gccgtgcagc
     3901 tggccgaggg ggtcaaggcc aaaggcatcg tcgtgatcac cagacgaggg ctcatggccg
     3961 atctcgtcgc aaactgccgc ccgttcgcca ccaacatcta cgcctttacc aacatgagcc
     4021 aaccgcgacg gaccatgatg ctcaatcgcg gcgt
//
LOCUS       DAOQRM010000203         4050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_24002_length_4050_cov_15.943429, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000203 DAOQRM010000000
VERSION     DAOQRM010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24002_length_4050_cov_15.943429"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKK81_12165"
     CDS             <1..495
                     /locus_tag="PKK81_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNV33686.1"
                     /translation="ETVNCVDPLGGSYFVESLTNRLEEGARDYFRTLDRMGGMVAAIE
                     QGFPQREILDASQRYQKEIEHKERPIVGVTDYVEQEARSIPILKIGPEVEQEQVARLS
                     DLRKSRDPFKMAGSLEELQETAACHQNLLPALIDAVKAKATLGEICAALKEVYGTYRE
                     PVVL"
     gene            492..1340
                     /locus_tag="PKK81_12170"
     CDS             492..1340
                     /locus_tag="PKK81_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV33687.1"
                     /translation="MKLGAFDIYAVTDGRFRLDGGAMFGVVPKALWQKCCPADELNRI
                     PLHLNCLLIQAHGKNVLVDTGLGDKEDAKFHSMFAVERMPSLRDSLQRHGLTHDDIHL
                     VINTHLHFDHAGGNTVANGNGLLQSAFPKATYYVQQGEYDDAVAANERTRASYRRNNF
                     SPVAQLNQWEFLRGDTELLPGITTMVTAGHTPFHQSVKVESEGRIAFFLGDLIPTVSH
                     LPLPYIMGYDLDPMQTLDTKRWVLDRAFAEDWLLIFEHDPLIQAGHLRKDTEGRYVLE
                     EVNVWQ"
     gene            1331..1738
                     /locus_tag="PKK81_12175"
     CDS             1331..1738
                     /locus_tag="PKK81_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNV33688.1"
                     /translation="MAIATTPIRVLIGKVGLDGHDRGVKLVVRALRDAGVEIIYTGLH
                     QTPEQIVTTAIQEDVQAIGLSIHSGAHNSLFPRVLQLLKEQGAGDVTLFGGGIIPDDD
                     VPRLKAAGVQMLFRPATPMHDIVDFVKGLRVER"
     gene            1752..3356
                     /locus_tag="PKK81_12180"
     CDS             1752..3356
                     /locus_tag="PKK81_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNV33689.1"
                     /translation="MRVLKSTVNKTSEDFRRAQAQYESLTADLKKHLAIVQAGGPADA
                     VALHKKRGKMTARERIAALLDRGSPWIELSPLAAFGLYDNQVPAAGLITGIGSVSGRF
                     CVIAANDATVKGGTYFPMTIKKHLRAQEIALENRLPAIYLVDSGGIFLPMQAEVFADK
                     DHFGRIFFNQARMSAAGIAQIAVVMGMCTAGGAYVPAMCDENVIVQGTGTIYLAGPPL
                     VKAATGEDVTSEELGGADLHTRLSGVSDHLAQDDHDALDICRSIIATLGRRPPRLRRE
                     KIDEPLYPAEELYGIIPANPRQKWEVREVIARLVDGSRFQEFKAGYGATLVCGFAHWM
                     GHLVGIVANNGVLLSESALKGAHFVQLCAQRRIPLVFLQNITGFMVGKDYETRGIIKD
                     GAKMVQAVATAEVPKFTILIGASHGAGNYAMCGRGYGPRFLFTWPNARTSVMGAQQAA
                     QVLVTVKQQQRAREQGRLSDEEQRRITEATRSQYEQEGSPYFSSARLWDDGILDPVET
                     RKVLGLCLDLAASVPARETHVPVFRM"
     gene            3383..>4050
                     /locus_tag="PKK81_12185"
     CDS             3383..>4050
                     /locus_tag="PKK81_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNV33690.1"
                     /translation="MRRYTTLAMESRGDIRCITLDRPERRNAFDSRMISELTQAFDES
                     AQEPSLRGVLLSSAGPVFCAGADLQWMQSTSPISEAQAQDDAHRLTRMFRAIDECPCP
                     VIARVQGPAFGGGLGLMAVCDIVVAAEDAMFSLSEARLGLIPAVIAPFLLRKAGESFL
                     RRYALTGETFTTSVAHRFGLVHDVVPQSDLDDRITEVIDAIRQLAPRATRATKGLILR
                     IRPLP"
BASE COUNT          848 a         1174 c         1249 g          779 t
ORIGIN      
        1 gaaaccgtca actgcgtcga tccgttgggc ggctcctact ttgtcgaatc gctgacgaac
       61 cgattggaag aaggagcgcg ggactatttc cgaacactgg atcggatggg tggaatggtg
      121 gcggcaatcg aacagggatt tccgcagcgg gaaatcctcg atgcgtcaca acggtatcag
      181 aaagaaattg aacacaagga gcggcccatc gtcggcgtca ccgattatgt ggaacaggaa
      241 gcacgctcca tccctatttt gaagatcgga ccggaagtgg aacaggaaca ggtcgcgcgc
      301 ttgtccgatc tcagaaaatc gcgtgatccc ttcaagatgg ccggctcatt agaggagtta
      361 caggaaacgg ccgcctgcca ccagaacctc ctgcccgctc tgattgatgc cgtgaaggcg
      421 aaagcgacat tgggagagat ctgtgccgca ttgaaggaag tctatggaac ctatcgagag
      481 ccggtggtgt tatgaaactc ggagcctttg acatctatgc agtgaccgac ggccggttcc
      541 ggctggatgg tggcgccatg ttcggggtgg tgccgaaggc gctctggcag aaatgctgcc
      601 cggcggatga gctgaatcgt attccgctcc acttgaactg tctgttgatc caggctcacg
      661 gcaagaacgt cctggtggat actggtctcg gcgataagga ggatgccaag ttccactcca
      721 tgtttgcagt ggagcggatg ccgtccctgc gcgactcgct gcagcgccat ggcctcaccc
      781 acgacgacat tcatctggtc atcaacaccc atctgcactt cgaccatgcc ggaggcaata
      841 cggtcgcgaa cgggaacggc ctgctgcagt cagcctttcc caaggccacc tactatgtgc
      901 aacagggcga gtacgacgat gccgttgcgg cgaatgaacg gaccagggcc agttaccggc
      961 ggaacaattt ctcaccggtc gcgcaactca atcagtggga gttcctccgc ggcgatacgg
     1021 aactgctgcc gggcatcacc acgatggtga cggccggtca tacgcccttt catcagagtg
     1081 tcaaagtcga gtcggaaggc cggattgcct tcttcctggg cgatcttatt ccgacggtct
     1141 cgcatctgcc cctcccctac atcatggggt acgacctcga ccccatgcag acgctcgaca
     1201 ccaagcgctg ggtgctggat cgagcgttcg cggaggactg gttgctgata tttgaacacg
     1261 atcccctgat ccaggccggt catctcagga aggatacgga aggacggtat gtgctggaag
     1321 aggtgaacgt atggcaatag cgacgacacc cattcgagtg ttgattggca aagtgggatt
     1381 ggacggacat gaccgggggg tcaaactcgt cgtccgggcg ttgcgcgatg ccggagtgga
     1441 gatcatttac accgggctcc accagacgcc ggagcagatc gtgaccaccg ccattcagga
     1501 ggacgtgcag gccatcggtc tcagtatcca ttccggcgcc cacaactcgc tctttccccg
     1561 cgtgctgcag ttgctgaaag agcagggcgc gggagacgtc acgctgttcg gcggaggcat
     1621 tatccccgat gacgacgtac cgcgactgaa ggccgccggt gtgcagatgt tatttcgccc
     1681 ggccacgccg atgcatgaca tcgtcgattt cgtgaaggga cttcgagtcg agcgttgaga
     1741 ggatgtccga gatgcgcgtc ctgaagagca cggtgaataa gacatcggaa gactttcgtc
     1801 gtgcccaggc acagtacgaa agcctgacgg ccgatttgaa gaagcacctg gccatcgtgc
     1861 aagcgggcgg accggccgat gcagtggcgc tccacaagaa acgcggaaag atgacggcac
     1921 gcgagcgcat tgccgccctg ctcgatcgag ggtcgccctg gatcgaactc agccccctcg
     1981 ccgcctttgg cttgtatgac aatcaggtcc cggccgcagg actgatcacc ggcatcggct
     2041 ccgtgtcggg acgattctgt gtcattgcgg ccaacgatgc gacggtcaaa gggggaacct
     2101 acttccccat gaccatcaag aaacatttgc gggcgcaaga gatcgccttg gagaaccggc
     2161 tgccggcgat ttatctcgtc gattccggcg gcatctttct acccatgcag gctgaggtct
     2221 ttgccgacaa agaccatttc ggacggatct tcttcaatca ggcgcggatg tccgcagccg
     2281 gtatcgccca gattgccgtg gtcatgggca tgtgcacggc cggcggcgcc tacgtgccgg
     2341 ccatgtgcga cgaaaacgtg atcgtccagg gaaccggcac gatttacctg gcaggaccgc
     2401 cgctggtcaa agccgcaacc ggcgaagacg tcaccagtga agagctcggg ggggccgatc
     2461 tccacacccg cctatccggt gtcagcgatc atctggcgca ggatgaccat gatgcattgg
     2521 acatctgccg atcgatcatt gccacgctcg ggcgacggcc tccgagactt cgtcgagaaa
     2581 aaatcgatga gcccctgtac ccggccgaag aactctacgg aatcattcct gcgaatccgc
     2641 gtcagaaatg ggaggtccgg gaggtgattg cgcggctcgt ggacggcagt cgcttccagg
     2701 aattcaaagc agggtacggt gccacgctgg tctgtggttt cgcccactgg atgggtcacc
     2761 tcgtggggat cgtcgccaac aacggggtgc tgctctcgga gtccgcgctg aaaggcgcgc
     2821 actttgtcca gctctgcgcg caacggcgta ttccactggt gtttctgcaa aacatcaccg
     2881 ggtttatggt cggaaaagat tatgagacga gaggcatcat taaggacggc gccaagatgg
     2941 tgcaggccgt cgcgaccgcc gaggtcccta agttcacgat cctgatcggc gcctcacacg
     3001 gagccggcaa ctatgccatg tgcgggcgtg gctatggccc tcgattcctc ttcacctggc
     3061 ccaacgcacg aacctcggtg atgggcgcgc aacaggccgc gcaggttctt gtcaccgtca
     3121 agcaacagca gcgcgcgcgt gaacagggac ggctgtcgga tgaagaacag cggcgaatca
     3181 cggaggccac ccggtcgcaa tatgagcagg aaggcagtcc ctatttcagc agcgcgcgac
     3241 tctgggacga cggcattctg gatcccgtgg agacgcgaaa agtcctgggc ctctgtctgg
     3301 atctggccgc gtccgttccg gccagagaaa cccatgtgcc ggtgtttcgt atgtgaggat
     3361 cgtggcggct gaggaggtac gaatgcgtcg atatacgacc ctggcgatgg aatcacgcgg
     3421 cgacatccgc tgcatcacgc tcgataggcc tgaacggcgc aacgccttcg acagccggat
     3481 gataagtgaa ctgactcagg cattcgacga atcggctcag gagccgtccc tgagaggagt
     3541 gttgctatcc agcgccggac cggtcttttg cgccggggcc gatctccagt ggatgcaatc
     3601 cacgtcaccg atctcggaag cccaggcaca ggatgacgcc catcgtctta cccgcatgtt
     3661 tcgcgcgatc gacgaatgcc cttgcccggt catcgccagg gtacagggac ctgccttcgg
     3721 cggcgggctc ggattaatgg cggtctgcga tatcgtcgtc gcggcggaag atgcgatgtt
     3781 ttcgctcagc gaggccaggc tcggtttgat tccggcggtc atcgcaccct ttctcctgcg
     3841 caaggcaggc gaatcgtttc ttcgacggta tgcgctgacc ggcgaaacat tcaccacatc
     3901 ggtggcccac cggttcggcc tcgtgcatga cgtcgtgcct cagagcgatc tagacgatcg
     3961 catcacggaa gtcattgacg ccatcaggca gctggctccc cgcgcaacca gggcaacaaa
     4021 agggttgatc ctgaggatac gcccgttgcc
//
LOCUS       DAOQRM010000204         3979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_24678_length_3979_cov_13.036442, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000204 DAOQRM010000000
VERSION     DAOQRM010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24678_length_3979_cov_13.036442"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKK81_12190"
     CDS             complement(<1..818)
                     /locus_tag="PKK81_12190"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV33691.1"
                     /translation="MSLLAWFAPRQETMIGRCATTGMILTILSVLPGCGEQGAEPPKP
                     PSPVEVAPQAAFTHAIHPPPAVRERLRTESATRRAVPELVTAPGEVALDLKKVAKITS
                     RIGGQVEHIHVQLGDRVKPAQPLAAIGSLQLDQLVEEYLVGKAQADVAENSLRRTEKL
                     RTDDIVPERKLIEDKGHYLETKARYQHVREKLLAMGISKAELAGLERGGHEEGHHYTL
                     TSPIAGTVVAQNAVRGQGVTPGAELFEVVDTSRVWVFANLPIEQARKFKEGDLGT"
     gene            complement(815..>1519)
                     /locus_tag="PKK81_12195"
     CDS             complement(815..>1519)
                     /locus_tag="PKK81_12195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV33692.1"
                     /translation="MKAGVEVQKAKKEVARANSTLLVAKARLNTLTAGSLGKEFSIQG
                     DFQSPKPGVNAEALATQAMEQHPAVRRLSKLAEQAEHTLRYEREARVPNISLLGSYHR
                     EAGDESVTAGLSMPLPLWYRRQGEIQSALGAKHRADAERLRQQNELEQAITQHAQEVR
                     TAQDQLQVFETGLLKQAEQTLTVARTSFRHGAASLLDVLDAQRVYRQTQLEYAQVRAD
                     LSIALARLERAVGASL"
     assembly_gap    1520..1619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1620..1876)
                     /locus_tag="PKK81_12200"
     CDS             complement(<1620..1876)
                     /locus_tag="PKK81_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33693.1"
                     /translation="MIARLVCSLVLAMAAAGPLAGVSVAWAEAPRTAAYSLPDILTLA
                     VQHNPTLAGAEGVVKQSQGQQIAAGAYPNPSVSGSAGRGAIR"
     gene            complement(1981..2466)
                     /locus_tag="PKK81_12205"
     CDS             complement(1981..2466)
                     /locus_tag="PKK81_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33694.1"
                     /translation="MRSTHSRFLRLDSLLRAWAVLWLLALPLFHIHPETDPHHGEAGH
                     IHAAAVHTVFSADLEGEFEDHHDAHHHAQDTDSEPVLSAQGPHTWENDHELGFSLLND
                     ATDRKLVKPLLAHLQFVRHNLLPVPEQRDQPAEQDPPILSNTILACDLPTRAPPSSLL
                     S"
     gene            2940..>3979
                     /locus_tag="PKK81_12210"
     CDS             2940..>3979
                     /locus_tag="PKK81_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNV33695.1"
                     /translation="MVSIFPLITIFAPLLGTLLISLFWRDLGDRVARLGIAALWCSAL
                     TSLATLAVVLSGDTIQLALIGAQSGAMTYTVMVDRLAAVMMALISLVSLIIHVYSERY
                     MVGDAGYVRFFALLGGLTCVLLSLVSSGNLFWLFVCWHLVTWLLARLLVCNPDSRAAR
                     DAGQKTFWVQSFGDAAFAVALLVLYGSYGTWDMTDLFARLQTAPAPLTVWPGLQWDCD
                     VPLVVTLLLVLSVMTKSAQFPFHFWLIGTIEAPTPVSALMHAGIVNAGGFLVNRLAPL
                     FGLAPTTLHLMFVIGGITALIGATAMLTQTSVKRRLVYSTMGQMGYMVMECGLGAFAL
                     AVFHLCAHGLFKA"
BASE COUNT          655 a         1137 c         1227 g          860 t
ORIGIN      
        1 gtgcccaggt cgccctcttt gaattttcta gcctgttcga tgggcaggtt ggcaaagacc
       61 cagacacggc tggtatcgac gacttcgaag agttctgccc cgggcgtgac cccctgtccg
      121 cgcaccgcat tctgtgccac caccgtaccg gcgatgggag acgtcagtgt atagtggtgg
      181 ccttcttcat ggccgccccg ttcgaggccg gcgagttcgg ctttcgagat gcccatggcc
      241 agcagttttt cgcggacgtg ctgatagcgt gccttcgtct ccaggtagtg ccccttgtct
      301 tcaatgagct ttcgctccgg cacgatgtcg tccgtgcgca gcttctcggt ccgtcggaga
      361 ctgttttccg ccacgtcggc ctgcgccttc ccgacgaggt attcttccac gagctgatcg
      421 agctgcaggc tgccgatggc ggcgagcggt tgagcgggct tgacccggtc acccaactgc
      481 acgtgaatat gttcgacctg cccgccgatg cgggaggtaa tcttggcgac cttcttcaaa
      541 tcgagtgcga cctcgccggg tgcggtcacc aattcgggaa cggcgcgtcg cgtggcggac
      601 tcggtgcgca accgttcccg gacagccggc ggcgggtgaa tcgcatgggt gaaggcggct
      661 tgcggcgcca cctcgacggg cgagggcggt ttcggcggtt ccgctccctg ctccccgcat
      721 ccggggagaa cagagaggat ggtcaggatc attccggtcg ttgcgcagcg tccgatcatc
      781 gtctcttgcc gaggtgcgaa ccaggcgagg aggctcatag cgaggctccc actgcccgct
      841 ccaaccgggc cagcgcaatg gagagatcgg cccgcacctg cgcatattcc agttgggttt
      901 gccggtacac gcgttgagca tctaatacat ccaacaaact ggcggcgccg tgccgaaaac
      961 tcgtgcgggc gacggtcaag gtctgttcgg cctgtttcaa caagccggtt tcaaatacct
     1021 gcagctgatc ctgcgccgtg cggacttcct gcgcatgttg agtgatggcc tgctccagct
     1081 cgttctgttg ccgcaggcgt tccgcatcgg cgcggtgttt ggcgccgagc gcggattgaa
     1141 tttctccctg ccgccggtac cagaggggga ggggcatact gagtccggcg gtgaccgatt
     1201 cgtcacccgc ctcccggtga taactgccca gcaggctgat attgggcacg cgggcttccc
     1261 gctcataccg cagcgtatgt tccgcctgtt ccgccagctt gctcaggcgg cggaccgccg
     1321 gatgttgttc catggcttgc gtcgccaggg cctccgcatt caccccgggc ttgggcgatt
     1381 gaaaatcgcc ctggatggaa aactcttttc cgagcgaccc ggcggtgagg gtattcaagc
     1441 gggccttggc caccaacagg gtactgttcg cccgggcgac ttccttcttg gccttctgca
     1501 cttccacccc ggctttcatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1621 gaatggcgcc acgcccggcg ctgccggaga cggacggatt cggataggcg cctgccgcaa
     1681 tctgttggcc ctggctttgt ttcaccaccc cctcggcgcc ggccagtgtg gggttgtgtt
     1741 gcaccgccag cgtcagaata tccggcaagg aataggcggc ggtgcgtggc gcttcagccc
     1801 aggctacgga aacgcccgcg agggggccgg cggcagccat tgccaggacc agggaacaga
     1861 caagacgagc aatcatcagc agcctcctcc gtgcgcaatc ggctcgcgat ggtgcgaggc
     1921 acaggcttcg ccctgcgagc gctatgcgat cagaatccag gatgtcagga cggacgacga
     1981 ttagctgagg agtgaggatg gcggcgcgcg cgtaggaaga tcacaagcga ggattgtgtt
     2041 ggagaggatc ggagggtcct gttcggcagg ctgatcacgc tgctccggaa cgggcaggag
     2101 gttatgacgc acaaactgca gatgggccaa cagcggcttc acgagcttgc ggtcggtggc
     2161 atcgttcagc agcgaaaagc ccagctcgtg atcgttctcc caggtatgcg gaccttgcgc
     2221 agaaagaaca ggctccgaat ctgtgtcttg cgcatggtga tgagcatcgt ggtggtcttc
     2281 gaattcaccc tcaaggtcgg ccgaaaagac cgtgtgcacg gcagccgcgt ggatgtgacc
     2341 ggcttcgcca tgatggggat ccgtctcagg atggatatgg aacagcggca aggccaacag
     2401 ccacagtacc gcccaggcgc ggagcaggct gtcgagtcgg aggaatcggc tgtgagtgga
     2461 acgcatgtgc atacgcttct aagtggacgc tatcacaagg agagaaacac atcaagaaac
     2521 gagcagggaa cgaggcgaat tctaaacaaa attcgaacgg gcaacaaagt cgtgtgctac
     2581 cggtgagatc tttccggcaa gttctctttt ttgcgtggta accgtagaaa acgattttca
     2641 tcagtgattg cccgtctgac tgcctctgtc ttctccgcgt ctcagattta gccgtgagac
     2701 gcatacgctt ccggatgctc tcttcctttc gttgacctcg ccaaccaagc tgtgcatata
     2761 tgcacgttcg tgcagtgatg cacgtcggtc gtgaggtcga accggcccct atgttgaatc
     2821 tctacgcgga gaggcttcaa attcgatgga attcccagta ttcgggcgat ccggcggcca
     2881 ggctctgttc ttgctgatga ccccgcacag atcacgcgtt tctcggagag tctgatccca
     2941 tggtctcgat atttcctctg attacgatct tcgcgccgct tctcggcacg ctcctgatca
     3001 gtttgttctg gcgtgatctg ggcgaccggg ttgcgcgtct cggcattgcc gcgctctggt
     3061 gttccgccct gacctcgctt gccacactgg cggtagttct gtcgggagac acgattcagt
     3121 tggccctgat cggcgcgcag tcaggcgcga tgacttatac agtcatggtg gatcgcttgg
     3181 cagcggtgat gatggcgctc atcagtctcg tgagcctgat cattcatgtg tactccgagc
     3241 ggtacatggt gggcgatgcg ggctatgtcc gcttcttcgc gctgctgggg ggactcacgt
     3301 gtgtgttgtt gagcctggtc agcagcggca acctgttctg gctgtttgtc tgctggcacc
     3361 tggtgacctg gttattggcg aggttgctgg tgtgcaaccc cgatagtcgt gcggccaggg
     3421 atgcggggca aaagaccttc tgggtccaga gcttcggcga tgccgcattt gctgtggcgc
     3481 tgctggtcct gtacggatcg tacgggactt gggatatgac cgacctcttt gcccggttgc
     3541 agacagcccc ggcgccgttg acggtctggc cgggtctgca atgggactgc gatgtgcctc
     3601 tcgtcgtgac cctattgttg gttctctcgg tcatgaccaa gtccgcccaa tttccgtttc
     3661 atttctggtt gatcggtacg atcgaagcgc cgaccccggt ttcggccctg atgcacgcgg
     3721 ggatcgtcaa tgccggaggg tttctcgtga accggctggc gcctctgttc ggtctcgcgc
     3781 cgacgacgct ccatttgatg tttgtcatcg gcggcattac ggccctgatc ggcgccacgg
     3841 cgatgctcac gcagaccagc gtgaagcgcc ggctcgtcta ttccaccatg ggtcagatgg
     3901 ggtatatggt catggagtgc ggcttgggtg cctttgccct cgccgtgttc cacctctgcg
     3961 cgcatggcct gttcaaggc
//
LOCUS       DAOQRM010000205         3932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_25111_length_3932_cov_14.511736, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000205 DAOQRM010000000
VERSION     DAOQRM010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25111_length_3932_cov_14.511736"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PKK81_12215"
     CDS             complement(<1..305)
                     /locus_tag="PKK81_12215"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HNV33696.1"
                     /translation="MHNAEGATSDDISSSSARVADSAVHPDSVGPGKAWGICTSVDLH
                     DCIPERIRDAKQIEAYVVQLCELIEMKRYGACQIVNFGEGRVAGYSMVQLIETSLISG
                     "
     gene            459..644
                     /locus_tag="PKK81_12220"
     CDS             459..644
                     /locus_tag="PKK81_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33697.1"
                     /translation="MADTSTPQSNPSAPTAELPERLCTWCKVPMTKRLVAGGQFIHYT
                     CPKCVFQHTTKRAPKTE"
     gene            complement(796..1503)
                     /locus_tag="PKK81_12225"
     CDS             complement(796..1503)
                     /locus_tag="PKK81_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNV33698.1"
                     /translation="MHTFSAEVSRIQDLTHDVRAIELRLLEPSSIAFKAGQFVSFEVP
                     KEGQPRPVTRPYSIASPPGQRDRILLVLNLVQGGPGSSYLFGLREGERASFKGPAGAF
                     YLRDEGVRDLLFVATGTGIAPLRSMILAQLERGESRSVTLFWGLRSQRDLYWQEELTA
                     LAADHPNFSFVTTLSRPDPGWEGASGRVTALIDAQVSSVGNLAVYLCGGSGMIKDVTA
                     RLNAKGLCPIYREKYYD"
     gene            complement(1530..2240)
                     /locus_tag="PKK81_12230"
     CDS             complement(1530..2240)
                     /locus_tag="PKK81_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNV33699.1"
                     /translation="MKILLLLLGGLKLGKIALTGGTMLLSMVVYSFIFGWWYAVGFVL
                     LILFHELGHYAAAKQRNLNVGAPTFIPFVGAWIQLKEQPMDVETEAYVGIAGPVAGTF
                     AAMACYYVAEYTHSQLMLALAYAGFMINLFNLIPLSPLDGGRITAIISPKVWWLGVPI
                     LIGLFVMNHSPMLLLIAIMALPHLMATFRGGPHGLPERYYDVPLSTRVSYGLYYLGLA
                     AFLGIMSYETHLLLPSRN"
     gene            complement(2237..3106)
                     /locus_tag="PKK81_12235"
     CDS             complement(2237..3106)
                     /locus_tag="PKK81_12235"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HNV33700.1"
                     /translation="MAVIVLVLAAWALPAIAEEPPQQALAWQVGPGEAKLGDQAVLKL
                     PKGYRFLGAQETQRLLKQMGNFPSGAELGLITATAENEQWFMVVRYIDAGYVKDDEAA
                     NWDADALMTSIKEGTDEDNKTRQAQGFPPLVIRGWEEKPHYDKAASKVVWAISAQERE
                     TVGVNYNTLALGRQGYLSMNMVGSLEQLPVLKPHVGLLLSNVEFIEGKRYTDFDSTTD
                     KVAAVGLSALIAGAAIKSGLLAKLWAFIIPLVIAGKKLLMLLVIALGGLAAKYFNKKP
                     KPEQAGGGGGLSS"
     gene            complement(3367..>3932)
                     /locus_tag="PKK81_12240"
     CDS             complement(3367..>3932)
                     /locus_tag="PKK81_12240"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNV33701.1"
                     /translation="VLRVVQLIYWNNSDRSTREALLAGAPALAMIGLLVGFYFLNWIP
                     PVPLSMKFGGMYHEVKRAGDHFELSYEKRWFQVWQRSNTTVAADEPIYCFTAVFAPVT
                     LKTTVYHHWYYRSDKSKPFSHADKIPLKIAGGREGGYRAYTFKQGLDVGDWRVDVETE
                     DGRVIGRVPVHVEKRDDASRSLTTIVY"
BASE COUNT          854 a         1259 c         1060 g          759 t
ORIGIN      
        1 ccgctgatca aggaggtctc gatcaactgc accatgctgt agcccgccac gcggccttct
       61 ccgaaattca cgatctggca ggcaccatac cgcttcattt cgatcagttc gcagagctgc
      121 acaacgtagg cttcgatttg tttggcgtcg cgaatgcgtt cggggatgca atcgtggagg
      181 tcaacggagg tgcaaatgcc ccaggctttg ccgggtccga cagagtccgg atggactgcg
      241 gagtcagcaa ccctggctga agatgatgag atgtcgtcgg aggtggcacc ttctgcgttg
      301 tgcatatgcg atacgacctt tctgttggga atggtgggag tgagaacaca tggttgcaat
      361 atactgaacg tttgaaaccc tgtaaatatt tatctccgaa atttctcgtg cttcccgcac
      421 attgacaaac tctcgacccc ttagcgagaa tgccgaccat ggctgatacg tcgacacccc
      481 aatccaatcc ttccgcaccg accgctgaat tgccggaacg tctctgcacc tggtgcaagg
      541 tccccatgac caagcgtctg gtcgcaggcg gccagttcat tcactatacc tgccccaagt
      601 gcgtgtttca gcataccacc aagcgcgccc ccaagactga gtaggtcacc tgcctcggaa
      661 ggggagaggc ctccaggctg atcaaagggg tcgcgaacaa ggccgcagcg agcgaacgac
      721 tgaggcatac tgttccggta tgtcgaagga gtgagcgacg cgagaacgcc gttggcggcc
      781 tctttcatcg gcctgctagt cgtaatactt ctcgcgatag ataggacaca gccctttggc
      841 attgagcctg gccgtcacgt ccttgatcat cccgctcccc ccacacagat agacggcgag
      901 attcccgacg gaggagacct gcgcatcgat caacgccgtc actcgcccgc tcgccccttc
      961 ccaccccggg tccggcctgg acagtgtcgt cacaaaagaa aaattgggat ggtccgccgc
     1021 cagcgccgtc agttcctcct gccaatagag atctcgctga ctgcggagtc cccagaacag
     1081 agtgacggat cgcgactccc cacgctccaa ttgcgccagg atcatgctgc ggagcggagc
     1141 aatcccggtt cccgtcgcga cgaacaacag gtctctcacc ccctcgtccc gcagatagaa
     1201 ggcaccggcc ggccccttga atgaagctcg ctctccttcc cgtaagccga acagataact
     1261 cgaccccggt ccaccctgaa cgagattcag cacgagcaga atccggtccc gctgacccgg
     1321 cggcgaagcg atggaatagg gcctggttac cggtcgaggc tgcccttcct tcggcacttc
     1381 aaatgacacg aactgcccgg ccttaaacgc gatgctggat ggctccagga ggcgcaactc
     1441 gatcgcgcgc acgtcgtgcg tgaggtcctg aatacgactc acttcagcgc tgaacgtgtg
     1501 cacagagacc taactcgtgg tgctgtcggt taattgcggg aagggagcag gagatgcgtc
     1561 tcataactca tgataccaag aaacgccgcc agcccgaggt agtacaggcc gtaactcacc
     1621 ctggttgaga gcggcacatc gtagtaccgt tccggcaggc catgggggcc gccccggaag
     1681 gtggccatca gatggggtaa ggccatgatc gcaatcaata agagcatggg gctgtggttc
     1741 atgacaaaga ggccgataag gatcggcacg cccagccacc agaccttcgg cgagatgatg
     1801 gctgtgatgc gcccgccatc cagcggtgag aggggaatga ggttgaacag attgatcatg
     1861 aacccggcat aggccagggc cagcatcaac tgactgtgcg tatactccgc cacatagtag
     1921 caggccatcg cggcaaaggt tcccgccacc ggaccggcga tgccgacata ggcctcggtt
     1981 tcgacatcca tcggctgttc cttcagctga atccaggccc cgacgaaggg aatgaacgtc
     2041 ggtgcgccga cgttcaggtt gcgctgcttg gcggcagcgt agtgccccag ctcgtggaac
     2101 aggatcaaca gcacgaaccc caccgcatac caccagccga agatgaaact atagaccacc
     2161 atcgacagca gcatggtccc gccggtaagc gcaatcttgc ccagcttgag accaccgagg
     2221 agcaggagga gaattttcac gacgacagcc ctccgccccc gcccgcctgc tctggcttgg
     2281 gtttcttgtt gaaatacttg gcggccaacc cgccgagcgc gatcaccagc agcatcaaca
     2341 acttctttcc tgcgatcacc aggggaataa taaaagccca gagcttggcc agcaggccgg
     2401 acttaatcgc cgcgccggcg atgagcgccg acaggccgac cgcggccacc ttgtcggtcg
     2461 tactatcgaa atcggtatac cgtttcccct cgataaactc cacattggac aacagaaggc
     2521 cgacatgcgg cttcagcacc ggcaactgct ccagggaccc gaccatattc atgctgaggt
     2581 acccctgccg ccccagtgcc agggtgttgt aattcacccc cacggtttcc cgttcctgcg
     2641 ccgagatggc ccagaccacc ttgctcgccg ccttatcgta atgcggcttt tcctcccaac
     2701 cacgaatgac cagcggggga aagccctgcg cctggcgagt cttgttatcc tcgtccgttc
     2761 cctctttgat ggaggtcatc agcgcatcgg cgtcccaatt ggccgcctca tcgtctttca
     2821 cataaccggc atcgatgtac cggaccacca tgaaccactg ctcattttct gctgtcgcgg
     2881 taatcaggcc cagctcggca ccggagggga aattccccat ctgcttgagc aggcgctggg
     2941 tctcctgcgc ccccagaaac cggtacccct tgggcagctt gagaacggcc tgatctccca
     3001 acttggcctc accaggtccg acctgccatg caagcgcctg ctgcggcggc tcctctgcga
     3061 tggcgggcag cgcccaagca gccagtacca gaacaataac cgccatgagc ctcagccgat
     3121 acctgaccat aaccggacct ccctatgccg cctgttcttt atcatggcgt gcggaaaacc
     3181 aagacaaaca catcaccgct gccgagattt ttaagatcac gactcgatca tcttagcgta
     3241 ttcgccgcac atgcccattc aaaacttgtt gaagccgtct gaatattgag cccaagcgtc
     3301 aacctttcct gtttatcgag gagttccagc cccttgatcc ggcgcatgtg cgactccagt
     3361 tgccggtcag tacacaatcg tcgtcaacga ccgcgatgca tcgtcccgtt tctctacgtg
     3421 caccggcacc cggccgatca cacgcccgtc ttcggtttcc acatccaccc gccagtcacc
     3481 gacatccaat ccctgcttaa acgtataggc gcgatacccg ccttcacgcc ccccggcgat
     3541 tttgagggga atcttgtcgg catggctgaa gggcttgctc ttgtcgctgc ggtagtacca
     3601 gtggtgatag accgtggtct tcagcgtcac tggcgcaaac accgcggtga agcaatagat
     3661 cggttcgtcg gcggccacgg tcgtattcga tcgctgccac acctgaaacc accgcttctc
     3721 atacgacagt tcgaaatggt cgccggcgcg cttgacctcg tggtacatcc ccccgaactt
     3781 catcgagagc ggcacgggag gaatccagtt cagaaagtag aacccgacca acaggccgat
     3841 catcgccagc gcgggcgcgc cggccagcag cgcctcgcga gtcgaacgat ctgagttgtt
     3901 ccaatagatc aactgtacga cgcgcaggac ca
//
LOCUS       DAOQRM010000206         3926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_25177_length_3926_cov_15.689486, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000206 DAOQRM010000000
VERSION     DAOQRM010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25177_length_3926_cov_15.689486"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKK81_12245"
     CDS             complement(<1..661)
                     /locus_tag="PKK81_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33702.1"
                     /translation="MKPWIKETFRVTVLTATLAGCGGDQGADMTDATVKVHMGGTALK
                     KSAMAAHFEVVELRKVAEKRISRTVHEYTYRVSVRNNGVSGAANVLAELLAVPAGTTI
                     VDGTVAVGSIGISTTVTPSDVIVLRIDRTLPFDPSNLSWKITSTDVQQLAPVKPAEVV
                     VLPLADIGVPENVDKVTVAGAVTDVLLKDGSLRFSTPGDTGAPQQAEFTLSGPNGSTV
                     LS"
     gene            complement(658..924)
                     /locus_tag="PKK81_12250"
     CDS             complement(658..924)
                     /locus_tag="PKK81_12250"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV33703.1"
                     /translation="MGRLFQGISMALAGGIALDDALGGFSVTFDYLGAGTPGSQLFSI
                     VDPNSFDELDSGARSSVPQPGTLPLMLAGLGALAYWQRRHGGVQ"
     gene            complement(1198..2949)
                     /locus_tag="PKK81_12255"
     CDS             complement(1198..2949)
                     /locus_tag="PKK81_12255"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HNV33704.1"
                     /translation="MSGHDSNVQSENSPDTPPRDVKNLVLCCDGTGNVWKPGPGKTNV
                     VKLFESLAGPRELQLTYYDPGVGTPDGAIGGDQGGGLSLLDILRRVGGLAWGDGVWAN
                     VAEAYEFLIRYYSAGDRIFLFGFSRGAFTARAVSGLIHMFGVLRPGHENLIPTLLRVY
                     RSRASNSRNTIAKSLKDQFSMPDTCVHFIGCWDTVESVGIWQFLGAHITSDAHVKSSF
                     RHVRHAMALDELRWPFKPRAYLPRANGLSNGTSYKQVWFRGAHSDIGGGYTDAGLSHI
                     TLHWMAREANEQGLMLDFDKLENGRQASPFALLHDAIAEMPTWIVAGAFKRELPQDLT
                     LHESVRRRAEYDPFRGFPQNAGQPDATTRKCITLPDGRISPRPVPAEPEATVLLPRRQ
                     PVARWVWPLLALAWIVTAVLFSSASNDSSRLAFDLQLGLDRQSWFELGKALANFEGST
                     RGKISELMRRDTGLIVAYSVALTLSSVLLLRFACHRNYTGVWLGRTSCIAGFSLTLAD
                     LTENWLTLQALEAYRTQACTWNWIPCTVSEPFLSLTVSLASGIKFIALAVVLLTSLCY
                     LAIGIRQRLQRLTSPFN"
     gene            complement(3125..3216)
                     /locus_tag="PKK81_12260"
                     /pseudo
     CDS             complement(3125..3216)
                     /locus_tag="PKK81_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611838.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3658..>3926)
                     /locus_tag="PKK81_12265"
     CDS             complement(3658..>3926)
                     /locus_tag="PKK81_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33705.1"
                     /translation="DGFGATADELRASEVTLTFENGYSVAGDLSTLILSSQFDPNRNL
                     DIITSRRGDPIAANVVPSPSTTSLLGLGLAMLGGFRLLFARSIT"
BASE COUNT          820 a         1062 c         1200 g          844 t
ORIGIN      
        1 gtgacagcac ggttgagccg ttcgggccgc tcagtgtgaa ttccgcctgc tgcggtgcac
       61 cggtatcgcc cggtgtcgag aagcgcaagc tgccgtcttt gagcagtacg tcggtgacgg
      121 caccggccac agtgactttg tcgacatttt ctggcacccc gatgtcggcc agaggtagca
      181 ccaccacttc ggctggtttc acgggagcca gttgctgcac gtcggtggac gtgatcttcc
      241 acgacaggtt ggacggatcg aacggcagtg tacggtcgat gcgcagcacg atcacatcag
      301 atggtgtcac ggtggtactg atgccgatgc ttccaaccgc aacagtgccg tcgactatgg
      361 tcgttccggc tggcacggcc agtaattcgg caagaacatt agccgcgccg gaaacgccat
      421 tattgcggac ggatacacga tacgtatatt catggacggt tctgctgatg cgcttctcgg
      481 ccaccttcct cagctcgacc acttcgaagt gcgcggccat ggcggacttt ttcagcgcgg
      541 tcccgcccat gtgcacttta accgtggcgt cagtcatgtc cgcgccctga tctccgccgc
      601 aaccggccag cgtggcagtc agtaccgtca cgcggaatgt ttcttttatc cacggtttca
      661 ttgcacacct ccatggcgac gttgccagta agccagcgcc cccagaccgg ccagcataag
      721 cggcaaagtg ccgggctgcg gcacgctgga gcgcgcgcca ctgtcgagct catcgaacga
      781 attcggatca acgatggaaa acagctgcga accgggtgtg cctgcgccca ggtagtcgaa
      841 cgtcacggaa aatccgccca gggcatcgtc gagggctatg ccgccggcca gcgccatcga
      901 aatcccctgg aacaaacgtc ccataagaaa tctccatttt taaagcaagg ggcaggagtg
      961 aatgcagaat gcattttttg tttagtctaa aacaactttt gtgcagatca gcctgaatcc
     1021 gtggccgcag cttttgtacc cggaagcctg ttcgcatagc gccacgaaat cgtttcgaat
     1081 tgaagtttga gcccggattg ggcatgagaa agcaccgcct gtacaggcag ctacgggctt
     1141 gcgccggctt tctctttgga tggacgcggg cggcgatgcc gatgatttcc tgggcagtta
     1201 attgaagggg cttgttaagc gttgcagtcg ttgtctgatg cctatggcga gatagcaaag
     1261 gctcgtgagg agcacgactg ctaaagcgat aaatttgatc cccgaggcca aggaaaccgt
     1321 cagggagagg aagggttcgc tgaccgtgca gggaatccag ttccaagtgc aggcttgtgt
     1381 gcgataggct tcgagggcct gcagcgtgag ccagttttcg gtcagatcgg ccaaggtgag
     1441 tgagaaacct gcaatgcagc ttgtccgacc cagccaaact cccgtgtagt tgcggtgaca
     1501 ggcaaaacgc agtagcagga cggaggatag ggtcagagca acggaatagg cgacgatgag
     1561 tcctgtatcc ctgcgcatga gttcggaaat cttgccccgc gttgagcctt cgaaatttgc
     1621 aagagctttg cccagttcga accacgactg cctgtcgagg ccaagctgga ggtcgaaggc
     1681 cagacgactg ctgtcattgc tggcgctgct gaaaagcacg gccgtaacga tccaggctaa
     1741 ggcgagcaaa ggccataccc accgcgcgac tggctgacgt ctgggcaaaa gcactgttgc
     1801 ctcaggttct gcgggaacag gccgaggaga gatgcgtccg tctgggagcg tgatgcattt
     1861 tcgggttgtg gcgtctggct ggcccgcgtt ttggggaaag ccccggaagg ggtcgtattc
     1921 tgcccgccgg cgtaccgatt cgtggagggt cagatcctgg ggcagctccc gtttgaatgc
     1981 gccggcaacg atccacgtag gcatttccgc gatcgcatcg tggagaagag caaagggtga
     2041 agcctgtcgg ccgttctcaa gcttgtcgaa atccagcata agcccctgtt catttgcttc
     2101 gcgggccatc cagtggaggg tgatgtgcga cagcccggcg tccgtgtagc cgcctccgat
     2161 atccgaatgg gcaccccgaa accagacctg tttgtagctg gttccgttgc ttagtccgtt
     2221 tgctctgggc aggtaggcgc gcggcttgaa gggccagcgc aattcgtcga gagccatcgc
     2281 gtggcgcacg tggcggaagc tgcttttcac atgggcatca cttgtgatgt gtgcaccgag
     2341 aaactgccag atgccgacgg attcgaccgt atcccaacat ccgatgaaat gaacgcaggt
     2401 gtcgggcatg gaaaactgat ctttcaggct cttggctatc gtgttacggg aatttgatgc
     2461 tctggagcga taaacgcgga gcagggttgg gattagattc tcgtgtccag gccggagcac
     2521 tccaaacatg tggatcagtc cggacaccgc cctggcggtg aaggcgcccc tggaaaaacc
     2581 gaagaggaat atccggtccc cggcgctgta atacctgatg aggaattcgt aggcttcggc
     2641 aacattggcc cagactccgt cgccccaggc tagtccgcca acgcggcgca ggatatcgag
     2701 aagactcagg cctccgcctt gatctccgcc tattgcgccg tccggcgtgc cgacgccggg
     2761 atcgtagtac gtaagctgga gttccctggg cccggccagg ctttcgaaaa gcttgaccac
     2821 gttggtcttt cccggaccgg gcttccaaac atttcccgtt ccatcgcagc agagaacaag
     2881 attcttgacg tcacgcggcg gagtgtcggg ggagttctcg gattggacgt ttgagtcgtg
     2941 gccgctcata gtctcctcct tcatctggtg tgtgctggtt tggacgctcg atatattgaa
     3001 aataggttct gatgcagctg atgtctatgc aaagggggtg caagaagctg ttcacgatct
     3061 tctgagggcg cgcgaggaaa gcgagatgga tgaactgcaa gctggtgttg agtttgcgct
     3121 ctcagttttt ccagagacgt cggccttttc gatccaggcg aatgatcgct ccgccacccc
     3181 gcgctggggc atgacggcga aagtgtgcaa ctcgctgctc aagcgggcgc gcttgattca
     3241 ctgcgcatga aattcatggg gtgacgtggc ccaaatggga aagtcagcgt gcgcgcccgc
     3301 gttttaattc cttgcgatcg ttcagtattt ccgcgcatct gacttcgtca tgtccggcgg
     3361 cgcgacaggc tgtgtattcg tcctttgatg gtttggatgc gggcgtaaga gtggaatact
     3421 cggcattcaa gcacgcaacg gaatcctgtc ctgattctct gcaaagtctg tactcttccg
     3481 agcagtgctt cagcttgtcc cggtttggat cacggatgca ggggatccag tcagggtcag
     3541 cggttacgcc actattcttg tctacagtcg cgccgggcgt gcccgccccc ttgccgagca
     3601 ctggggtggt cgcgaaggag gctgccgctg cgagaatcaa aaagaatgaa gaagttttca
     3661 tgtgattgat cgggcgaaaa gcaatcggaa gccgccgagc atcgcaagac caagtccaag
     3721 cagtgaagtc gtcgacggcg aaggcacgac atttgctgcg attgggtcgc cacggcggga
     3781 tgtaatgatg tccagattgc gattgggatc aaattgcgat gacaggatca gcgtggacag
     3841 atcaccggca accgaatatc cattttcaaa cgttagcgtg acttcagacg cacgcagctc
     3901 atcagcagtg gcgccgaacc cgtcaa
//
LOCUS       DAOQRM010000207         3921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_25239_length_3921_cov_17.549664, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000207 DAOQRM010000000
VERSION     DAOQRM010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25239_length_3921_cov_17.549664"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PKK81_12270"
     CDS             <1..701
                     /locus_tag="PKK81_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNV33706.1"
                     /translation="YSALVPNERGLERARAAGVDKIAVFTAASNSFTRHNIKATVQES
                     LVRFRPLVAAAKRDGMAVRAYLSTVVFCPYEGRIAPTQVRDVMERLLDLAVDDISLGE
                     TIGKASPTDIRRLLDTVLPHVEPARLSLHFHDTYGMAVANALTAWWDYGITAFDASAG
                     GLGGCPYAPGASGNVATEDLVFALKASGANVPVDELLVAACAQQLGDAVRHPLNSRLS
                     QLHHREKPQPAWRE"
     gene            754..1476
                     /gene="meaB"
                     /locus_tag="PKK81_12275"
     CDS             754..1476
                     /gene="meaB"
                     /locus_tag="PKK81_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNV33707.1"
                     /translation="MARQVLAGGVRAVARVITWIENQEPVGAAVLKRLTPPEKRAMVI
                     GITGYPGTGKSTLIDQLIAAYRRLGKTVGVLAVDISSPLTGGAVLGDRIRMQRHADDA
                     GVYIRSMATRGQSGGLARATRDALQVLEAAAYDVILIETVGVGQSDIEVVSVAGTVIA
                     LVAPGLGDDVQAMKAGLLEVADIVVVNKGDREGADTAVRDLREWCPHVLRTVAVKGEG
                     IPELIAMIAEQQRLQDLAHTPG"
     gene            1506..2909
                     /locus_tag="PKK81_12280"
     CDS             1506..2909
                     /locus_tag="PKK81_12280"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HNV33708.1"
                     /translation="MLADRLASYTQALCYDDLPGDVVHEVKRRILDSAACAFGAWKAT
                     PCRIARHVAQSAKVPGGATIWGTSHKTLPDLAAFANGALVRYLDFNDTYLSKEPAHPS
                     DNLAAILAAGEIAHASGKRVIQAIALAYEIQCRLCDAAALRSRGWDHVTYGSISSALG
                     AAKTLKLTEAQTTQAINLAAVANVALRQTRVGEMSMWKACAFSNAARNGLFAAILAQR
                     GMTGPAPIFEGEKGFMKLVSGPFELSRLGGERAPDDTLASFKILDSYIKHFPVEYHAQ
                     TAVEAALALRAEVIKAEGADAIQRLSEIEIGSYDVAIEIIGRDPEKWHPTTRETADHS
                     FPYCVAVALLDGRVTLHSFDPHRLRDAALHELMKKVRVVPQPEFAGRYPGAMPTRLTL
                     RTTTGTVYMRHVDVPLGHPGNPMSDQDVEDKLRRLASRRIGLAQVEKLIGFVWTLERE
                     KDIATLMPLLRVPHRDA"
     gene            2902..3843
                     /locus_tag="PKK81_12285"
     CDS             2902..3843
                     /locus_tag="PKK81_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNV33709.1"
                     /translation="MRNHSTPSKAQRLRGLLAGRTVAIPGACNALTAMQIERAGFEIV
                     YVSGAAISAAHGMPDIGLISLTDMTREASAIARSVALPTIVDADTGYGPPSVVREAVQ
                     EFERAGLAGMQIEDQEEAKKCGHLSGKRLIPTADMVAKITAAVEAKCDRDFVLVARTD
                     ARTVDGLQAAIQRGVAYANAGADALFPEALLSADEFHTFALEMNRAGVHLPLIANMTE
                     FGKTPYLSVDEFETLGYRGVLFPVSTLRVAALAVEKLLRELRFFGSQRAWLDHMMTRQ
                     ELYSLLRYEHGQNQTGRPYEPGDTRTARNENHGAPPL"
BASE COUNT          774 a         1190 c         1252 g          705 t
ORIGIN      
        1 cctattcggc cctcgttccg aacgaacgcg gcctcgaacg ggcgcgagcg gcaggcgtcg
       61 acaagatcgc cgtcttcacg gctgcctcga acagctttac ccgccataac atcaaagcga
      121 ccgtgcagga gtcactcgtg cgcttcagac ccctcgtggc ggcagcgaaa cgagacggca
      181 tggcggtgag agcctacctg tccacggtcg tgttttgtcc ctatgaaggc cgcattgcac
      241 ccacgcaggt gcgggacgtg atggaacggc tgctcgacct cgcggtcgac gacatctcgt
      301 tgggcgaaac gatcggcaag gcttcgccca cggacatacg gagattgctc gatacggtgc
      361 tgccgcatgt cgagccggct cgcctctcgc ttcatttcca tgacacctat ggcatggccg
      421 tcgccaacgc cctcacggcc tggtgggact atggaatcac ggcgttcgat gcttcggccg
      481 gaggcctcgg cggctgtccc tacgccccag gcgcatccgg aaatgtcgcg acggaagatc
      541 tggtcttcgc gctgaaggcc tccggcgcca acgtgccggt ggatgaactg ctggttgccg
      601 cctgtgcgca acaactcggt gacgccgtgc ggcatccgct gaactcgcgg ctttcgcaac
      661 tccatcatcg tgaaaagcca cagcctgcgt ggcgggaatg acatgaatca cgcgtgcgtc
      721 ggtcgattat cgaaacggga ggatgtcgag accatggcca ggcaagtgct ggccggtggg
      781 gtccgggctg tggcacgcgt gatcacctgg attgagaacc aggagccggt tggagccgcc
      841 gtgttgaaac gcctgacgcc accggagaag cgcgccatgg tgatcggcat cacgggttat
      901 cccggcacgg ggaagagcac gttgatcgat caactcatcg ccgcctaccg ccgcctcggc
      961 aaaacggtcg gcgtcctggc cgtggatatc agcagccctc ttaccggcgg ggcggtgctg
     1021 ggtgaccgga tcaggatgca gcggcacgcg gacgatgcgg gggtctacat acgcagcatg
     1081 gcaacccgcg gtcagtccgg tgggctcgcg cgggcgactc gcgatgcgct gcaagtcctg
     1141 gaagcggccg cgtatgacgt gatcctgatc gaaacggtcg gcgtcggcca gagcgacatc
     1201 gaggtcgtct ctgtggcggg aaccgtcatt gccctggtcg cgccgggatt gggcgacgac
     1261 gtgcaagcca tgaaggcagg gttactggag gtcgcggata tcgtggtcgt caacaaaggg
     1321 gatcgtgaag gcgccgatac tgcagtacgc gatctacgcg agtggtgtcc gcacgtgctg
     1381 cgcaccgtgg ccgtcaaggg agagggcatt ccggagctga tcgccatgat cgccgaacag
     1441 caacggctcc aggatctcgc acacacaccg ggctgacaga acggcttgtt gtcgagagga
     1501 tcatcatgct ggcggatcgt cttgcgagtt atacccaggc cctttgttac gacgacctgc
     1561 ccggcgacgt ggtccatgag gtcaagcgac ggattctcga cagcgccgcc tgtgccttcg
     1621 gcgcctggaa ggcaactccc tgccggatcg cgcgtcacgt ggcacaatcc gccaaagttc
     1681 ccggtggtgc gacaatctgg gggacgtccc acaagaccct gccggacctg gccgccttcg
     1741 ccaacggcgc gttggttcgc tacctcgact tcaacgatac ctatctctca aaagagccgg
     1801 cccatccttc ggacaacctt gccgcaatcc tcgccgccgg cgaaatagcc catgcttcgg
     1861 gcaagcgggt catccaggcc atcgccttgg cctatgaaat ccaatgccgt ctctgcgacg
     1921 ccgccgcgtt acgctcccgt ggatgggacc atgtgaccta tggatcgatc tcctccgcgc
     1981 tcggcgcagc caaaacgctg aaactgacgg aggcgcagac cacgcaggcc atcaacctgg
     2041 ccgccgtggc gaacgtggca ctccggcaaa ccagagtcgg tgaaatgtcc atgtggaaag
     2101 cctgtgcgtt ctcgaatgcc gcacgcaacg gcctattcgc ggccatcctg gcccaacgag
     2161 gcatgacagg ccccgccccc atctttgaag gcgaaaaggg cttcatgaag ctggtgtccg
     2221 gaccattcga gctgtcgaga ctgggtggag agcgggctcc cgacgacacc ctggcttcgt
     2281 tcaagattct cgattcctac atcaaacatt tcccggtgga ataccatgcc cagactgcgg
     2341 tcgaagcggc gctggccctg cgagcggagg tgatcaaggc ggagggggcc gatgcaatcc
     2401 agcgtctgag cgagattgaa atcggcagtt acgatgtcgc gatcgaaatt atcgggcgcg
     2461 atccggagaa gtggcacccg acgacgcgcg aaacggccga tcacagcttc ccctattgtg
     2521 tcgcggtggc gttgctggat ggtcgcgtca cgttgcattc attcgaccct caccggctac
     2581 gagatgctgc gctgcacgag ctcatgaaga aggtgcgtgt cgtcccgcag ccggaattcg
     2641 cgggccgtta cccgggagcg atgccgaccc gcctcacgct gaggacgacg acgggaacgg
     2701 tgtacatgag gcatgtggac gtccccctgg gccatcccgg caatcccatg tcggaccagg
     2761 atgtggagga caaactgcgc cggctcgcat cgagacgaat cggtctcgcg caggtcgaga
     2821 agctcatcgg tttcgtgtgg acattggaac gagaaaaaga catcgccaca ctgatgcctc
     2881 tgttgagggt gccccatcgt gatgcgtaac cactccacgc cgtcaaaagc acaacgcctg
     2941 cgtgggctgc tggccggcag gacagtggcc atccccgggg cctgtaatgc cctgacggcc
     3001 atgcagatcg agcgcgccgg attcgagatc gtgtatgtgt cgggagccgc catctcggcg
     3061 gcccatggca tgcccgatat cggattgatc agcttgacgg acatgacgag ggaagcctcg
     3121 gcgattgccc ggtcggtggc gcttcccacc atcgtcgatg cggacaccgg ctatgggccg
     3181 ccttcggttg tgcgggaggc cgttcaggaa tttgagcggg ccggattggc aggcatgcag
     3241 atcgaagatc aggaagaggc gaaaaaatgc ggccatctgt cagggaagcg actgattcca
     3301 acggctgaca tggttgccaa aatcaccgca gccgtggagg cgaaatgcga tcgggatttc
     3361 gtccttgtgg cgcgcacgga cgcacggacg gtcgatgggc tgcaggctgc cattcagcga
     3421 ggcgttgcct atgccaacgc aggcgccgac gcgctcttcc ccgaggccct gctctccgcg
     3481 gacgaattcc acacctttgc gttggaaatg aaccgggcag gcgtccacct gcccttgatc
     3541 gccaacatga cggagttcgg caagacacct tacttgagcg tggacgaatt cgagacgttg
     3601 ggatatcgcg gcgtgttatt tcccgtcagc acgctccggg tcgcagccct tgctgttgag
     3661 aagctgctgc gagaactcag attcttcggc tctcaacggg cttggctcga tcacatgatg
     3721 acgcgacagg agctctatag tctgcttcga tacgagcatg ggcagaacca gacagggagg
     3781 ccctatgaac caggcgatac aagaacagcc cggaacgaaa accacggagc gccccctcta
     3841 tagccccgga cttgaagggg tgatcgcggg agaatcggcc ctctgtcagg tggatgaagg
     3901 agaggcagga cttcgttatc g
//
LOCUS       DAOQRM010000208         3893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_25499_length_3893_cov_14.809536, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000208 DAOQRM010000000
VERSION     DAOQRM010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25499_length_3893_cov_14.809536"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKK81_12290"
     CDS             <1..714
                     /locus_tag="PKK81_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNV33710.1"
                     /translation="GNYDDVNRLCSEIAGEHGWAFVNINIRPYYAEGSKTLAFETVEQ
                     LGWRTPDQAVIPMASGSLLTKIWKGLHEMHALGLVDSVRTKINGAQAEGCSPIATAFK
                     AGRDFFKPVKPKTIAKSLAIGNPADGYYALKATAESKGAMDAVTDEEVVEGIKLLAQT
                     EGIFAETAGGVTIGVLCKLVKQGLIKKSDVTVAYITGNGLKTQEAVVDAVGRPFRIQP
                     SLVNFQKTFKMGKNSGGDS"
     gene            711..983
                     /locus_tag="PKK81_12295"
     CDS             711..983
                     /locus_tag="PKK81_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNV33711.1"
                     /translation="MIKVRIPTPLRPLTKGQGEVESAAANIVDMIGSLDATYPGLKNR
                     LCDEKGDLRRFVNIYVNEEDIRFLNGKETSLKDGDEVSIVPAIAGG"
     gene            986..1225
                     /locus_tag="PKK81_12300"
     CDS             986..1225
                     /locus_tag="PKK81_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HNV33712.1"
                     /translation="MTSLRFHIRFPENKIKEPIIYQIGHEFKVVTNVRRADVRETTGW
                     MDLELTGETEEIERAIDGIRTKGCVVDPIELNVVE"
     gene            1311..2114
                     /gene="moeB"
                     /locus_tag="PKK81_12305"
     CDS             1311..2114
                     /gene="moeB"
                     /locus_tag="PKK81_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNV33713.1"
                     /translation="MEFTEQQIQRYSRHIILNEVGGKGQVKLSKAKVLLIGAGGLGSP
                     AALYLAAAGIGTIGLVDGDVVDLSNLQRQILHTTATIGVPKVESGRRMLSAINPDITI
                     KTYQLNVDTENILGLVSDYDIVLDGSDNFTTRFLVNDACFFARKTLISASMFRFEGQL
                     TTIKPHAGYPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILGIGE
                     TIADKLVIYDALEMKFRKVSRPKDPRCPLCSATPTIKDLGGDYSVACTI"
     gene            2116..2628
                     /locus_tag="PKK81_12310"
     CDS             2116..2628
                     /locus_tag="PKK81_12310"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNV33714.1"
                     /translation="MPDLSIPQHILDQIVAHARELAPFECCGLLAGTNKTVTHLYRIT
                     NIVAVEGAEKLSTFDDDKVAHLERLSPEERAEIAFVMDMGDFSAAKKDIRKNGLDLQV
                     VYHSHPKDPARPSHTDIKIATDYEEIWERINLPVPAYLLVSLMHSPAADIRTYWIADG
                     KVRPADVHIM"
     gene            2729..3058
                     /locus_tag="PKK81_12315"
     CDS             2729..3058
                     /locus_tag="PKK81_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33715.1"
                     /translation="MGFGLLIVALILCLTGVTNPEHTSAAEKSYYSPIINVDVDNSRI
                     LISTLGAVFWVEVPEEAKAHIEKLPQSGLVDIVVETREGQPPLLKTWKVKSGESTCLH
                     FDGKVCK"
     gene            complement(3161..3370)
                     /locus_tag="PKK81_12320"
     CDS             complement(3161..3370)
                     /locus_tag="PKK81_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33716.1"
                     /translation="MAGSTGRKTVSVSRGLMMLCDVCYEQVFAENLVDQRNFVSDHES
                     LFDTICMGCTDINRPLVDDMLGSSE"
     gene            complement(3416..3610)
                     /locus_tag="PKK81_12325"
     CDS             complement(3416..3610)
                     /locus_tag="PKK81_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33717.1"
                     /translation="MVLIYESDPLDNEDARGHFYHVCRGRIDRTEIRLPEGESIYECA
                     VCGVDLEPEDFWIARQKGSV"
BASE COUNT          914 a         1179 c         1026 g          774 t
ORIGIN      
        1 ggcaactacg acgatgtgaa ccggctctgc agcgaaatcg ccggtgaaca tggctgggcc
       61 ttcgtgaata tcaacattcg tccctactat gccgagggct cgaagaccct tgcgtttgaa
      121 acggtggaac aactcggctg gcgcacgccg gaccaggccg tcattcccat ggcctctggt
      181 tccttattga cgaagatctg gaagggcctg cacgaaatgc atgcgctggg gctggtcgat
      241 tccgtccgca ccaagatcaa cggtgcgcag gctgaaggct gctccccgat cgcgaccgcc
      301 ttcaaggcag gccgggactt tttcaagccg gtcaagccga agaccatcgc caagtccctc
      361 gccatcggca acccggccga cggctactac gccttaaaag ccaccgcaga aagcaaaggc
      421 gcgatggacg cggtgacgga cgaggaagtc gtggaaggca tcaagctgct ggcccagacc
      481 gaaggcattt tcgccgagac cgccggcggc gtgaccatcg gggtcttgtg caagctcgtg
      541 aagcaggggc tgatcaagaa gagcgacgtc acggtcgcct atatcaccgg caacgggctc
      601 aagacccagg aagcggttgt ggacgccgtc ggtcgtccgt tccgcatcca gcccagcctg
      661 gtgaacttcc aaaaaacatt caaaatggga aagaacagtg gtggtgactc atgattaagg
      721 ttcgtattcc aacgcccctg cgtcccctca caaagggtca gggcgaagtt gaatccgcgg
      781 cagccaacat tgtggacatg atcggatcgc tcgacgcgac ctatccgggg ctgaagaacc
      841 ggctctgtga cgaaaagggc gacttgcgcc ggttcgtcaa catctacgtc aacgaagagg
      901 acatccgttt cctgaacggg aaagaaacct ctttgaagga cggcgatgaa gtgtcgatcg
      961 ttcctgcgat tgccggagga tagccatgac cagcctacgc tttcatattc gttttcccga
     1021 gaacaagatc aaggagccga tcatttatca gatcggccat gagttcaagg tcgtgaccaa
     1081 cgtgcgtcgg gccgatgtgc gggagacgac cgggtggatg gatctcgaac tgaccggcga
     1141 gaccgaagaa atcgaacgcg ccatcgacgg catccgcacc aaaggctgcg tcgtcgatcc
     1201 cattgaattg aacgtagtgg aataacaatg ggtgaagcgc acctcgtgaa gtgtatctcg
     1261 ttttagggcc agaccgcttc acgtttcacg aacgacgaga gacgagtcga atggaattta
     1321 ccgaacagca aatacagcgc tatagccggc acatcatcct caacgaggtc ggcggcaagg
     1381 gacaggtcaa actgtccaag gccaaggtcc tcctgatcgg agccggggga ctcggctcgc
     1441 ccgcagccct gtatctggcg gctgccggca tcggcaccat cggcctggtg gacggcgacg
     1501 tggtggatct ctcgaacctc cagcggcaga ttctccatac gactgccacc atcggcgtgc
     1561 ccaaggtgga atccgggaga aggatgctgt cggccatcaa ccccgacatc acgatcaaga
     1621 cctatcagct aaacgtcgat accgagaata ttcttggact cgtgtccgac tatgacatcg
     1681 tgctggacgg ctccgacaat ttcaccacgc gcttcctcgt gaacgatgcc tgtttcttcg
     1741 ccagaaagac actcatctcg gccagcatgt ttcgcttcga gggacagctc acaaccatca
     1801 agccgcatgc cggctaccct tgctaccggt gtctctaccc ggaaccgccg ccggccggat
     1861 tggtcccgaa ctgtcaggaa gccggtgtat tgggggtcct ggccggaacc atgggcatcc
     1921 tgcaagcctc tgaagccatc aaggaaattc tgggcatcgg cgagacaatc gcggacaagc
     1981 tcgtgatcta cgatgcgctg gagatgaagt tccggaaagt gtcgcgcccg aaagacccgc
     2041 gttgccctct ctgcagcgcc acgccgacga tcaaagactt gggcggggac tacagcgtcg
     2101 cctgcaccat ctgatatgcc cgatctatcg attccgcaac acatcctgga tcagatcgtc
     2161 gcgcatgcgc gcgagttggc gccgttcgaa tgctgcggcc tgctcgccgg cacgaacaag
     2221 accgtcacac acctctaccg catcaccaac atcgtggccg tcgaaggcgc ggaaaaactc
     2281 tccacctttg atgacgacaa ggtcgcccat cttgaacggc tgtccccgga agagcgagct
     2341 gagatcgcct tcgtgatgga catgggtgat ttttcagccg ctaagaaaga catccgcaag
     2401 aacggcttgg accttcaagt ggtctaccat tcccatccga aagacccggc gcgcccctcg
     2461 cacaccgaca tcaagatcgc caccgattac gaagaaatct gggaacgcat caatctcccc
     2521 gtccccgcct atctcctcgt gtcactcatg cactcccccg cagcggatat tcgcacctac
     2581 tggatcgccg acggcaaggt tcgccctgcc gacgtgcata tcatgtagtc atcgcgccgc
     2641 tagaacaaaa ccatctcgct ccctctacca atcgaccggc aacgatgcca taattgtgct
     2701 ttgaccttgt catcgaaggg agcattcgat gggatttggc ctgctcattg tcgcactcat
     2761 tctgtgcctg accggggtga cgaaccctga acacacctcc gccgctgaaa agagctacta
     2821 cagcccgatc atcaacgtag acgtcgataa cagccgcatt ctgatctcaa cgctcggcgc
     2881 ggtgttttgg gtggaggtcc cggaggaggc caaagctcac atcgagaagc taccgcaatc
     2941 agggctcgta gatatcgtgg tggaaacacg cgaagggcaa ccgccgctgc ttaaaacttg
     3001 gaaggtcaag tccggcgaat cgacctgcct gcatttcgac ggaaaagtct gcaagtaaag
     3061 ttgagccgtc gtcgctggtg cctgtcggtg gcccaagttc cgacaggcac acatgtagag
     3121 acggtctgtt atgacccgga agtcatgcgg tagcgctacg ctactccgaa ctccccaaca
     3181 tgtcgtcgac taacggtcga ttaatatccg tacaccccat acaaatagta tcgaacaacg
     3241 attcgtgatc ggacacgaag ttgcgctgat cgaccagatt ctcagcaaag acctgttcat
     3301 aacacacgtc acacagcatc atgaggccgc gtgacacgga cactgttttc cggcctgtac
     3361 tccccgccat aacacactcc ctttcgtata tatgctccgg tgcgcgcagc ctgcgctaca
     3421 ccgatccctt ttgcctcgcg atccagaaat cttcaggctc tagatcaaca ccacacaccg
     3481 cgcactcata aatcgactct ccctccggca accggatttc cgtccggtcg atcctgcccc
     3541 gacagacatg ataaaaatgc ccccgggcgt cctcattgtc gagcggatca ctctcgtaaa
     3601 tcagcaccat atcccttact ccctttgatc atcattcatg aagtctgcgc aaccgtaaca
     3661 ctcaatattc aatcgactct tggatattga ccacgtattt cttatacata ttcgtaagga
     3721 tgctcaaaat ggccgtccag caaggccgca gcgacgtgaa tgagcgaggc gtacccttgt
     3781 ggtacgttga gctctttcac gatgcgagaa cgatgctggc ggactttttc agcatcctgt
     3841 taaattttaa actgggcctc atacagcccc gcatacaccc cgccccgttt cat
//
LOCUS       DAOQRM010000209         3877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_25660_length_3877_cov_14.465986, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000209 DAOQRM010000000
VERSION     DAOQRM010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25660_length_3877_cov_14.465986"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..448
                     /gene="sugE"
                     /locus_tag="PKK81_12330"
     CDS             134..448
                     /gene="sugE"
                     /locus_tag="PKK81_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HNV33718.1"
                     /translation="MAWTLLCVAGLFEICWAIGLKYTEGFTRPWPTVGTLVAMAASFG
                     CLAQALKTIPVGTGYAVWTGIGAAGTAVLGMVLFAETASTIKVLSLLCIVLGIIGLRS
                     TS"
     gene            490..1677
                     /locus_tag="PKK81_12335"
     CDS             490..1677
                     /locus_tag="PKK81_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HNV33719.1"
                     /translation="MLPLTTPTQHESPLLHFPNRNTPKPTGWTLGEWLLLLPVGTAAL
                     FFHYGHEWLSDLSSPVRLSAILGWLLLVIILSAFAVLRHAEHLAERLGEPMGTVILTL
                     SVTGIEVMMIAAFMYTGNGNASLARDAMFAVVMIVLNGMVGLSLLLGGLRYHEQTYNL
                     QGANAFLAVIVPLAVLGLVLPSYTISSPGPTFSPHQSTFLIVMSLGLYGVFLAIQNLR
                     HRDYFVAPRSQSKRKMALLHQTEQAPKTPVWRHTLLLVAYLLPLVILSEHVATPIDYT
                     LRLWKAPHALGGVLVAGLVLAPESLGAVRAALANQLQRSVNILLGSVLASISLTIPAV
                     LAIGFFTESTIILGIDTVDTILLVLTFVVSMLTFAVRRTNVLLGAVHLLLFLAYLMLI
                     FEK"
     gene            1867..3288
                     /locus_tag="PKK81_12340"
     CDS             1867..3288
                     /locus_tag="PKK81_12340"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV33720.1"
                     /translation="MSGTKHKVFTILLVEDDAGDVDMFLRTVEHELPRHEDEQVTLVI
                     NATAEGALERLQQQPIDLIIADVRLPGMSGIELLRRVQDLNRRIPVIIVSWVHAIETV
                     IDAMRHGAFDYIVKPYDTMDLSARIHRAMRMSEILLQASPDEPAAPRIPFKNLVGVSS
                     PIRNVMAMIEAIARVPSTTLIIGETGTGKELIAKAIHERSLDSDGPFQVVDCTTFSEG
                     TVESELFGHVRGAFTGAVADRTGLIESGSHGTVFLDEIGDLPANLQAKLLRVLEAGEV
                     RAVGSTQQRKVNVRFIAATNQDLAEKVKRNEFRKDLFFRLNVMVIRVPPLRERTEDIP
                     VLARHFIARYAKEFTKRVEDLHPSAVTELVAYPWPGNVRELRNVMERAVMLAKGDRIG
                     IADVAGMLAVPDERQREAPEEDYLHLPYAKAKEQVLEAFNQRYISTKLTIHQGNVTHA
                     AQDAGLPRSYFHEIMRRYTKDEK"
BASE COUNT          812 a         1178 c         1046 g          841 t
ORIGIN      
        1 atgtccctag tgatatgccg aggttactcg gaagagatga tcgtgtacca tctctagcct
       61 gggagtgttt ccatgttgac gttgatgctc cctctctaag ccatacgtca cgaaggtcga
      121 tgtaaccgaa actatggcgt ggactttact gtgtgtcgcc ggactctttg aaatctgttg
      181 ggccatcggg ttgaagtaca ccgaaggatt tacccggccg tggcccacag tcggaacatt
      241 ggtcgccatg gcagcgagct tcggctgcct ggctcaagcc ttgaaaacaa tccccgtggg
      301 aaccgggtat gcggtatgga ccggtatcgg cgcggcaggc accgctgtcc tcggcatggt
      361 cctctttgca gaaactgcct cgaccatcaa agttctctcg ttgctctgca tcgtactcgg
      421 catcatcgga ctcagaagca cttcctgagc caggcggcag cgatgttcat tggcggattc
      481 acgtagacca tgttgccact cactacaccc actcagcacg agtctccgtt actccacttc
      541 ccgaatcgca acaccccgaa gccgaccggc tggacgcttg gagagtggct cctgctcctg
      601 cctgtcggaa ctgccgccct gttctttcac tatggccatg agtggctgag cgacctctct
      661 agtcccgttc gattgagcgc catcctcggc tggctcctgc tcgtgattat tctctccgcc
      721 tttgccgtcc ttcgtcatgc ggagcacctt gcagagcggc tcggcgaacc gatgggaacc
      781 gtgattctga ccttgtcggt caccgggatc gaagtgatga tgatcgccgc ctttatgtat
      841 acgggcaacg ggaatgcgtc gctggcgcgt gacgccatgt ttgcggtggt catgatcgta
      901 ctgaacggca tggtcggcct ctcgctcctg ctcggcggcc tgcggtatca cgaacagacc
      961 tacaacctac aaggggcgaa cgcgttcctc gcggtcattg tcccgctggc ggtgttgggg
     1021 ctcgtgctgc cgagttatac gatttcctca cccggtccga cattttcccc ccatcaatcc
     1081 acctttctca tcgtgatgtc cttggggttg tatggtgtgt ttctcgcgat tcagaatctg
     1141 cgccaccgcg actacttcgt ggcaccccgg agccagagca agcgaaaaat ggccttgctc
     1201 catcagactg aacaggcccc gaagacacct gtctggcgcc atacactcct cctggtggcc
     1261 tacctgcttc cgctcgtcat tctctcggaa catgtggcga caccgatcga ttacacactt
     1321 cgcctgtgga aggcccccca tgcgttaggc ggtgtgctgg tcgccgggct ggtgctggcc
     1381 cccgaatctc ttggcgcggt ccgggccgct ctggccaatc aattgcagcg ctccgtcaat
     1441 attctccttg gatcggtgct cgcgagtatc agcctcacca ttcctgccgt cctcgctatc
     1501 ggcttcttta ctgaatccac catcattctc gggatcgaca cagtcgatac catcctcctg
     1561 gtcctgacct tcgtggtcag catgctgacc tttgccgtca gacgcaccaa tgtcctgctc
     1621 ggagccgtcc acctgctcct gttcctggcc tacctcatgc tgatttttga gaaatagcgt
     1681 cgggatacgg ccctgcttgg agtgggccac ggagttgatt tgcctcgcct tcgcctccta
     1741 agatacgggc cagaggacag caattcacgc aacgcactca tggccgaccc cgggccagtc
     1801 tgaaccgcac cagatcaaaa aaccattgct gtgagcggac tgtaggagtt tccgagaaag
     1861 acacgcatgt caggcaccaa gcacaaagtc ttcacgatcc tgctggtcga ggacgacgcg
     1921 ggcgacgtgg acatgttctt gcgcacggtc gagcatgaac tgccccgcca tgaggatgag
     1981 caggtcacgc tggtcatcaa cgccacggcc gaaggcgcgc tggagcggct gcaacagcaa
     2041 ccgatcgatc tgatcattgc cgacgtgcgg ctgccgggca tgagcggaat cgaactcctg
     2101 cggcgggtac aggacctgaa ccggcgtatc cccgtcatca tcgtcagctg ggtccatgcg
     2161 atcgagacgg tcattgacgc gatgcgccat ggagcattcg actacatcgt gaagccctac
     2221 gacacgatgg atctgtcggc gcgcattcat cgggccatgc gcatgtcgga aatcctcctg
     2281 caggcctcac cggacgagcc tgccgcgcca cgcattccct tcaagaacct ggtgggtgtg
     2341 agctccccga tcagaaacgt gatggccatg atcgaagcca tcgcccgggt cccctccacc
     2401 acactgatca tcggggaaac cggcacgggt aaggaattga tcgccaaggc gattcacgag
     2461 cgcagtctcg atagcgatgg cccgttccag gttgtcgact gcaccacctt ctcggaaggc
     2521 accgtggaga gtgagttgtt cggccatgtc cgcggcgcat tcaccggcgc agtggcggac
     2581 cgaaccggct tgatcgagtc aggcagccac ggcaccgtct ttctcgacga aatcggcgac
     2641 ctccccgcca atctgcaggc gaaactcctg cgtgtgctgg aagccgggga agtgcgggcg
     2701 gtcggcagca cccagcagcg gaaggtgaac gtccgcttta tcgccgcgac caatcaggac
     2761 ctggctgaga aggtaaagcg gaacgagttc cggaaagacc tgttctttcg cctcaatgtc
     2821 atggtgatcc gcgtcccgcc tctccgtgaa cggacggaag atatccccgt gctcgcccga
     2881 cacttcattg cacgctatgc caaggagttt acgaaacgcg tggaggatct ccatccctcc
     2941 gccgtcacag agctcgtcgc ttacccatgg ccggggaatg tccgggaatt acgaaacgtg
     3001 atggaacggg cggtcatgct cgccaagggc gaccgcatcg gtatcgcgga tgtagccggc
     3061 atgctcgccg tgccagacga gcgacagcgc gaggcccccg aagaagacta tcttcacctg
     3121 ccgtatgcaa aagccaagga acaggtactg gaagccttca accaacggta catctcgacc
     3181 aaactgacga tccatcaggg gaacgtcacc catgccgccc aggacgccgg actgccacgt
     3241 tcctacttcc atgagatcat gcgccgctat acaaaagacg aaaaatagcg gtgtcagcaa
     3301 agcatgacac ccgcccacgc gaaaacaata taagcaattg ttttcattga ataaaccaac
     3361 aataaacctg cgcgtccgtg tttacagaca cagataaatc tttatatttc aacatcttag
     3421 tctgtacatc tcccatagta gtcagcagtt actgacccct gagttttcgt catccattgc
     3481 ccttcacttc cccatcccaa gacgaatcgc aacatactga ttttattaat tttaattcct
     3541 cattaccaag acgaaacgga gccacatttt tggccatctt cggcacgtga attgtaatac
     3601 tcctacaccg cgagcgaatg tccgctcgac aatttcgaat tgcgaggggc gaatgtttcc
     3661 aattcgacac acggaaagga ggtactccag acaagattgc atccacgtga gtgtagtgag
     3721 gttgtacgaa caggtgcatg agggactcat gtgcctcaca aggaggtagc tattatgcag
     3781 gtttccgttt cacggagaca gtttctcaaa atctcggcgg gaaccgtcgc ggccgtggcc
     3841 gtggcggata aagtcctcgc gttgaccgcg ctgcagc
//
LOCUS       DAOQRM010000210         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_26271_length_3814_cov_16.205906, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000210 DAOQRM010000000
VERSION     DAOQRM010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26271_length_3814_cov_16.205906"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="PKK81_12345"
     CDS             <1..1793
                     /locus_tag="PKK81_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV33721.1"
                     /translation="PVHLDRVWGSIFSFVGGRFVTKWPERVPYPVTVSFGTPVPAETP
                     AHELRRLVRELGEAAWQLRKPTRRPLHQAFISTMRRHPFRLAMADATKPHVSSLQALI
                     GAIALARALKTHWQGQQNVGLLLPPTVAGALTTVAATLAGRTCVNLNYTVGKAGLESA
                     IRQAHLGTIVTSRKFIEKAKLELPEGPTILWLEDIGATIGTRDKLTAAALAVLAPLRL
                     LESACGQTERVTMDHLATIIFSSGSTGEPKGVMLSHFSIDANVQAVSQVLPLAEDDRI
                     LGILPLFHSFGYLVFWYVTLNGAATVFHPSPLDVTAIGELCTKHRLTFLVCTPTFLQL
                     YQRRCTPEQFSTLRVVLTGAEKLPLRLCESFQDRFGIGPIEGYGVTECAPVIAVNCPD
                     FRAAGFFQPASRRGTVGQPLPGVSTRIVDPETFAILPPGTAGMLLVKGPNVMNGYLGR
                     EDLTAQAMRGGWYITGDIATLDDDGFLTITDRLSRFSKIGGEMVPHRLVEEALQLASG
                     EELQICAVTGVPDERKGEQLAVLHTLPEERIPQILAKAAAGGLPNLFLPSRTHCIKVE
                     ALPILGTGKLDLRALKRIAMERLGEREGSS"
     gene            1793..2833
                     /locus_tag="PKK81_12350"
     CDS             1793..2833
                     /locus_tag="PKK81_12350"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV33722.1"
                     /translation="MHEERETNDEQRDPPPKATPQGQTLIDCHVHLAALPDGDNGCYI
                     SPKTLKSPLFRFLLWKHGLSPDKPREANHKYLADLLTELKASRHVHKAVLLGMDGTYD
                     HNGRYNQAHTDFLIGNDYVLKTAQAHPTEFLAGVSINPQRRDAIDEVHRCADAGAILV
                     KVLPNAQQFDPADRKYTPFYRALAERKLPFLSHVGYEFSLIGKDQSVGEPDRLRLALE
                     EGATVIAAHACSYGLILYEKFLPTLRDLVTRYPHFYADISALTLPNRMRMLLHLRQYP
                     ELHERLLFGTDYPLSVFHMAAWGRVALGTLRKMIGTKNRFDRQVEVCNGLQLGFRSLG
                     DLLPHSSGMAGH"
     gene            2845..3342
                     /locus_tag="PKK81_12355"
     CDS             2845..3342
                     /locus_tag="PKK81_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV33723.1"
                     /translation="MTRDEILTSLRERILAFATSRVSKDLAEDLTQEVLVLLHEKYAR
                     VTELTELVPLAFQVLRFKMLDAHRKSLRRGEYNQESVDELPLANPGDDPATLLDQKQR
                     VDRLLAAVAQLGDRCRDLLTLKLEGHSFPEIQTRMGQHSINTIYTWDLRCRKQLLSLM
                     GGTWE"
     gene            3397..>3814
                     /locus_tag="PKK81_12360"
     CDS             3397..>3814
                     /locus_tag="PKK81_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33724.1"
                     /translation="MPEHDLEKLLGGFAANTLTPEERSQLYTASLQDQQLFNALADEQ
                     ALKELLSDPAVRRKLLEALKRPNTAEAGGSLPWWAWFKRPAGLAFAGGLAAVIFALAF
                     GTRIYQESLDRAAQSVATEDARQAGPSVRTPPSADSP"
BASE COUNT          761 a         1234 c         1075 g          744 t
ORIGIN      
        1 taccggttca cctggatcgc gtctggggca gcattttcag ttttgtcggg gggcgattcg
       61 tcaccaaatg gccggagcgt gttccttatc cggtcaccgt ttcgttcggg actccggttc
      121 ccgccgagac gccggcgcac gaactccgcc gcctcgtccg cgaactcggg gaagctgcct
      181 ggcagttgcg gaaaccgacc cggcgcccgt tgcaccaggc cttcatcagc accatgcggc
      241 ggcatccctt ccgtctcgca atggccgatg cgacgaaacc ccacgtgtct tcgctccagg
      301 ccttgatcgg ggcgatcgcc ctggcacgcg ccctgaaaac ccactggcag ggccaacaaa
      361 atgtgggcct gctgctgccg ccgaccgtgg caggagcgct gacgacagtc gccgccacac
      421 tcgcgggccg cacctgcgtc aacctcaact acaccgtggg caaagccggg ctggaatcgg
      481 ccatccgcca ggcgcacctg ggcaccatcg tcaccagccg taagtttatc gagaaggcca
      541 aactcgaact cccggaaggc cccaccattc tctggctgga ggacatcggc gccaccatcg
      601 gcacgcggga caaactcacc gccgcagccc tcgctgtact ggctccgctc cgtcttctgg
      661 aatcggcctg cggacagacc gagcgcgtga ccatggatca cctcgccacc atcatcttca
      721 gcagcggcag caccggcgag ccgaaaggcg tgatgttgtc tcacttcagc atcgacgcga
      781 atgtgcaggc cgtctcgcag gtgctccccc ttgccgagga tgatcgaatc ctgggcattc
      841 ttcccctctt ccactcgttc ggctacctcg tgttctggta cgtcacgctc aacggcgctg
      901 cgaccgtgtt tcatccttca ccgctcgatg tgacggcgat cggtgaactc tgtaccaaac
      961 accgcctcac atttctggtt tgcaccccca cttttctgca gctctatcaa cggcgctgca
     1021 cgccggaaca gttcagcacg ctacgagtcg ttctcacggg ggcggagaaa ttgccgcttc
     1081 gcctttgcga atcgttccaa gaccgcttcg gtatcggccc gatcgagggc tatggcgtga
     1141 ccgaatgtgc gccggtcatc gccgtgaact gcccggattt tcgcgccgca ggatttttcc
     1201 aaccggcctc gcggcgtggc accgtcggcc aaccgctgcc cggtgtctcc acccgcatcg
     1261 ttgatccgga gacctttgcg atccttcccc ccggcacggc tggcatgctg ctcgtcaaag
     1321 ggccgaacgt gatgaacgga tatctcggtc gcgaggattt gacggcccag gccatgcgcg
     1381 gcggatggta tattaccggt gacattgcca cgctcgacga cgatgggttt ttgaccatca
     1441 ccgatcggct ctcacgtttt tcgaagatcg gcggtgaaat ggtgccgcat cggctggtgg
     1501 aagaggcgct ccaactagca tcaggcgagg aactacaaat ctgcgccgtc accggcgtac
     1561 cggatgagcg taaaggtgag cagttagctg tattgcatac gcttcctgaa gagaggatcc
     1621 cccagatcct tgccaaggcc gcagccggcg gcctcccaaa tctatttctg ccctcgcgca
     1681 cccactgcat taaagtcgag gcactcccga tcctgggaac gggcaaactg gatctcagag
     1741 ccctcaagcg gatcgcgatg gaacggctgg gggagcgtga aggctcatcg tgatgcacga
     1801 agaacgagag acgaacgacg aacaacgaga tccgcccccg aaggccacgc cgcagggaca
     1861 gacgctgatc gactgccatg tccatctggc cgcgctgccg gacggcgaca acggttgcta
     1921 tatttcaccc aagacgctga agagcccgct gttccggttt ctgctgtgga aacacgggct
     1981 ctcaccggat aagccacgcg aggcgaatca caaatatctt gccgacctcc tgacggagct
     2041 gaaggcctca cgccacgtcc acaaggccgt gctgctcggc atggacggca cctatgacca
     2101 caacgggcgc tacaatcagg cacacaccga ctttctcatc ggcaacgact atgtcctcaa
     2161 gaccgcgcaa gcccatccca ctgaattcct ggcgggcgtc tctatcaatc cgcaacggcg
     2221 ggatgccatc gacgaagttc atcgttgcgc cgacgcgggc gcaatcctgg tcaaagtcct
     2281 gcccaacgcg caacagttcg acccggccga tcggaaatac acccccttct accgcgccct
     2341 ggcggagcga aagctgccgt ttctcagtca tgtcggctac gaattcagcc tcatcggcaa
     2401 ggaccagtcc gtgggcgaac cggaccggtt gcgcctggcg ctggaggaag gcgccaccgt
     2461 catcgcggcc cacgcctgca gctatggctt gattctctac gagaaatttc ttccgacctt
     2521 gcgcgacctc gtgacgcgtt atccgcattt ctatgcggat atttccgccc tcaccttgcc
     2581 gaaccgaatg cggatgcttc tgcacttgcg gcagtatccg gagctacatg aacgcctgct
     2641 cttcggcacc gactatccgt tgtcggtatt tcatatggca gcctggggcc gggttgcact
     2701 cgggacattg cgcaagatga tcggaaccaa gaaccgcttc gaccggcagg tcgaagtctg
     2761 caatggccta cagctcggat tccgctccct cggagaccta cttcctcatt catcaggaat
     2821 ggcgggccac tgagtattct tgccatgacg cgagacgaga tactcaccag cctgcgcgaa
     2881 aggattctgg ccttcgcgac atcacgtgta tcgaaggatc tcgccgaaga tctcacgcag
     2941 gaggtgttgg tcctgttgca tgaaaagtat gcccgcgtca ccgaactgac cgaactggtc
     3001 cccctggcct ttcaggtgct ccggttcaaa atgctggatg cccatcgcaa gtcactgcgc
     3061 cggggcgaat acaatcagga atcggtcgat gagctgccgt tggctaatcc cggtgacgat
     3121 ccggcgacac tattggatca gaagcagcgt gtcgatcgac tgttagccgc cgtggctcag
     3181 ctcggggatc gctgccgcga cctcctcaca ttgaaattgg aaggacacag tttcccggag
     3241 attcaaacgc gcatgggaca gcattcgatc aacaccattt acacctggga tctccgctgc
     3301 cgaaaacagt tgttgagttt gatgggcggc acgtgggaat gatgatgtcg gccctcattt
     3361 cacgtcttac gtttcacgta ttacgcagta acagacatgc ctgaacacga tctcgaaaaa
     3421 ttgctcggtg gattcgcagc gaatacgctg acgccggagg agcgctcgca actctacaca
     3481 gcctccctgc aggatcagca attattcaac gccctggcgg atgaacaggc gctgaaagaa
     3541 ttgctgtccg atcccgccgt gcgtcggaag ctgctggagg ctctgaagcg gcccaacaca
     3601 gccgaagccg gtggatcgct tccctggtgg gcatggttca agcgaccggc cgggctcgcc
     3661 ttcgcgggtg gtctcgccgc agtgatcttc gcgctcgcat tcgggaccag aatctatcag
     3721 gaaagtctcg atcgagccgc ccaatcggtc gcgactgagg acgcccggca agccgggcca
     3781 tcggtccgaa cgcctccatc ggccgactca cccc
//
LOCUS       DAOQRM010000211         3796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_26460_length_3796_cov_17.153970, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000211 DAOQRM010000000
VERSION     DAOQRM010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26460_length_3796_cov_17.153970"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2198)
                     /locus_tag="PKK81_12365"
     CDS             complement(<1..2198)
                     /locus_tag="PKK81_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNV33725.1"
                     /translation="MRVRPRVVVLSLLGLVVVGALLLLYSRELFGVDVIKNFFLQQLE
                     TSLRRKIEVDRIKLVVLPSVRLELSNVGVYGHDDPTHVVFQAKEIDIVLRLLPLLKKQ
                     VVAKRIFLDEPTVTLIRNRSGHWNVLAGLPSAAKDESAYQMFSRLLQIREATIQQGHI
                     TITDEARPDGVRTTKLEAVEMALKVYPGKAQGDLHISASLPAEAAPSSFSLTGTIGLS
                     ESSSSLAAEEPYSVHPAFQFDGEIETTNLRLREAADFFGPRPVPAQLQGGANLQSRIR
                     VAPGVAGYDVVLTEIAANVDQLAITGKANMAGLLTSQPTFSITFASPTIDLKTLFARM
                     PPQWIHPQLPGIVEQRQLGGTVEIVSATLTGATAPSPQLSLTGDFRIEKGTALIGNDR
                     VPTQDLSATISVEPGRIRVGKVTGSYGTLQMTDGKAVVSFLDEGPWMELDISGNMTAA
                     DLVKFLTKTIRADRLTSLLAQSREIEGQTHPTFRLVGPLDKPEGITFAGGEVLAEQVS
                     LVNPSLPQRLTAMHGRILFSQTGGAQFDQVTANVGETQLQFNGMISGGTPSVFQDFVI
                     RAKGSAAQLRQMVSAGTFPDDLLYGMVNAKVQLSGPSGAPHLRGEIGLNDAKLVLPMV
                     GEKPLGSPASLELDADVTRGVGLVISRMELVVPPLRLPLKGHITLGDQFSIDASLASG
                     TVSLSSLPEWIYRSGFEAGNLEISMDVKGTDAEWRNWRTGGWLALTNGLMS"
     gene            2446..3690
                     /locus_tag="PKK81_12370"
     CDS             2446..3690
                     /locus_tag="PKK81_12370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV33726.1"
                     /translation="MGAGRATTQPRWGILGLLFAISAVTYMDRVNISVTARQMMPAYG
                     LTDQDMGYIFSAFVFGYALCQIPGGWLGDRWGARVVLAGALVWWSLFTALTAVAATLP
                     LAAMVGTVGALIVVRFLLGVGEAVALPNFNRAVADWIPPAQRGLGIGIAIGGIGIGAA
                     ITPPLASWVMVNYHWQTVFYLSALIGLVVALLWVLCSREQKAADGVASIHPRVVVPWR
                     QFAASSSLRWLVLSYACLGYVAYIYMSWFYLYLVNVRGIDLLRGGWLAAAPFVAILVF
                     CPLGGWTTDRLVSTLGLTKARMIVGMIGMGLAGGLIAVGAWADSQAVAIASLSLGAGW
                     LYFTVGAYWSVTTDLSKTHAGTLSGVMNMGANVGGVISPSLTPWLADHWGWTASLLVA
                     ALIALCGGVMWMKIDASEGLRE"
BASE COUNT          658 a         1110 c         1254 g          774 t
ORIGIN      
        1 gacatcaggc cgttggtgag cgccagccat ccgcccgtgc gccagtttct ccattcggca
       61 tccgtgccct ttacatccat ggagatttcg agattgcccg cctcgaaccc gctgcggtag
      121 atccattcgg gaaggctcga caacgacacc gtaccggagg cgagggaggc atcgatcgaa
      181 aattgatcgc ccagggtgat gtgtcctttt aacggcaacc gcaacggcgg taccacgagt
      241 tccatgcgag aaatcacgag gccgactccc cgcgtgacat cggcatcgag ctccagggag
      301 gccggcgaac cgagcggctt ctccccgacc atcggcagca cgagtttggc gtcgttcagg
      361 ccgatctctc cgcgcagatg cggcgcacca gacggaccgg ataactggac cttcgcgttg
      421 accatgccat agaggagatc gtcggggaag gttccggcag agaccatctg gcggagctgg
      481 gcggcgctgc ccttggcgcg gatgacaaaa tcctgaaaga cgctgggcgt cccgccgctg
      541 atcatgccgt tgaactgcag ttgcgtctcg cccacgttgg cggtgacttg atcgaattgg
      601 gctccgccgg tttgcgagaa caggatgcgc ccgtgcatgg ccgtgagccg ttgcggcagg
      661 gagggattca ccaggctgac ctgctcggcc agcacttccc cgcctgcgaa tgtgatacct
      721 tccggtttgt ccagcggacc gacgaggcgg aaggtcggat gggtctgtcc ttcgatctca
      781 cgcgattgcg cgagcaatga cgtgagccgg tccgcgcgaa tcgtcttggt cagaaacttc
      841 accagatccg ccgccgtcat gttgccgctg atgtccagct ccatccaggg cccttcatcc
      901 aggaacgaca ccacggcttt tccgtcggtc atctggagcg tgccgtagct ccccgtcacc
      961 tttccgaccc ggatacgtcc cggctccacg gagatggtgg cggacagatc ctgcgtgggc
     1021 acacggtcgt ttccgatcag cgccgtgccc ttctcaatcc ggaagtcgcc tgtgagcgac
     1081 agttgcgggc tgggcgcggt ggcgccggtc agcgtggccg agactatttc gaccgtcccg
     1141 cccagttggc gctgttcgac gatgccgggc aattggggat gaatccattg cggtggcatg
     1201 cgggcgaaca gcgtcttgag atcgatggtg ggcgacgcaa aggtgatgga gaacgtgggt
     1261 tgcgaggtca ggagtcccgc catgttcgcc ttgccggtga tcgccagttg atcgacattg
     1321 gcggcgatct ccgtgagcac cacgtcatac ccggccacgc cgggcgccac gcggatgcgg
     1381 ctctgcagat tggcgccacc ctgcagttga gcgggcacgg ggcgcgggcc gaagaagtcg
     1441 gccgcttcgc gcaggcgcag gttggtggtt tcgatctcgc cgtcgaactg aaacgcggga
     1501 tggacggaat agggttcttc cgccgccagc gacgaggagg actcgctcaa gccgatggtg
     1561 ccggttaagg aaaatgaaga aggggccgct tctgcgggca aagaggcgga gatgtgcaag
     1621 tctccctggg ccttgccagg atagaccttc aatgccattt cgaccgcttc cagcttcgtg
     1681 gtgcggacgc cgtccggacg ggcctcgtcc gtgatcgtga tgtgcccctg ctgaatcgtg
     1741 gcctcacgga tttgcaggag acggctgaac atctgatagg ccgattcgtc tttggccgca
     1801 gaggggagcc cggcgagcac attccaatgg ccggagcgat tgcggatcag cgtgacggtc
     1861 ggctcgtcga gaaagattcg tttggccacg acctgcttct tcagcagcgg caagaggcgc
     1921 aggacgatgt cgatttcttt ggcctggaag acgacgtggg tcgggtcgtc gtgaccatag
     1981 acacccacat tggagagttc gagccggacg ctgggcagga cgaccagctt gatccggtcg
     2041 acctcgatct tgcgccgcag actggtttcg agttgctgga ggaagaaatt cttgatgacg
     2101 tcgacgccga acaactcgcg cgaatagagg agaaggagcg cacccacgac caccaggccg
     2161 agcagcgata acacgacaac gcgcggacga accctcactc cacctccatc aggtaggtca
     2221 aaatctgagt gtactggatg catgagaggg aaatccatac ggcagagagg cgctatcggg
     2281 cgtgaaacgt gaaacgagaa aggtgaaacg tgaggcgtca cacgtgagac gtgaaacggt
     2341 aggagaccgt atctcgttgg ggatcgcaaa tacctttcgt cggctggtgc gtgccggtct
     2401 ataatgcctc gctttctgac gggtgagtaa aaaccggggg tacgcgtggg agcaggcaga
     2461 gcgacgacac aaccgcgatg gggcattctc gggctcctgt tcgcgatcag cgccgtgacc
     2521 tacatggacc gggtcaacat ctccgtcacc gcgcgacaga tgatgcccgc ctatggcctc
     2581 accgaccagg acatgggcta tatcttttcc gcctttgtgt tcggttacgc gctctgccag
     2641 attccaggcg gctggctggg cgatcgctgg ggagcccggg tggtgctggc gggtgcgctg
     2701 gtctggtggt cgttgttcac ggccttgacg gcggtggccg caaccctgcc gttggccgcc
     2761 atggtcggga cggtcggtgc gctgatcgtc gtgcgctttc tcctcggtgt cggagaagcg
     2821 gtggctctgc cgaatttcaa tcgggccgtg gccgattgga ttccgcctgc gcaacgtgga
     2881 ctcggcatcg gcattgccat cggcggcatc ggcatcggcg ccgcgatcac ccctcccctc
     2941 gcctcctggg tcatggtgaa ttaccactgg cagacggtct tctacctctc cgcgttgatc
     3001 ggtctggtgg tggccctgtt atgggtgctc tgctcccgag agcagaaagc ggcggatggc
     3061 gtggcgtcca tacacccgcg cgtagtcgtt ccctggcggc aattcgccgc ttcgtcatcc
     3121 ctgcgctggc tggtgttgag ttacgcctgc ctgggttacg tcgcctacat ttatatgtcc
     3181 tggttttatc tctacctggt gaacgtccgc ggcatcgatc tgttgcgtgg cggctggctt
     3241 gcggctgcac ctttcgtggc gatcctcgtc ttctgcccgc tcggcggctg gaccacggat
     3301 cgcctggtct cgaccctcgg tctgacgaag gctcgaatga tcgtcggcat gatcggcatg
     3361 gggctggccg gtggactgat cgccgtcggc gcctgggccg actcacaggc cgtggccatc
     3421 gcctctctct cgctgggtgc cggctggttg tatttcaccg tcggggccta ctggagcgtg
     3481 acgaccgatc tctctaagac ccatgcaggc accctgtcgg gtgtgatgaa tatgggcgcg
     3541 aacgtaggcg gcgtcatttc tccgagcctt acgccctggc tcgccgacca ctggggttgg
     3601 acggcttcgt tactcgtggc cgcgctcatc gccctctgtg gcggcgtgat gtggatgaag
     3661 atcgatgcga gcgaggggtt gagggagtga ggcggtggcg agggattggt gtgcgcataa
     3721 cgaacgcgga tgcgggacta tttgcgccgt cgctcatcct cgatttagta tgacatgata
     3781 tcgggttccg atagtt
//
LOCUS       DAOQRM010000212         3790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_26524_length_3790_cov_15.376439, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000212 DAOQRM010000000
VERSION     DAOQRM010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26524_length_3790_cov_15.376439"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PKK81_12375"
     CDS             <1..679
                     /locus_tag="PKK81_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33727.1"
                     /translation="FWWFFEYLNRFVQNWHYVGGGELSAWEYLVRATLPFSTVLPAVL
                     GTAEWLSSFPRLSAGLDRFVTFEFKNHTTWGWGLLIGSAVGLFGIGLWPDYLFSLVWV
                     APLLLITSLQLIRDEPTICSGTAQGDWRTLWAAALAALICGWFWEMWNFYSLARWEYA
                     VPFVQRFKLFEMPLLGYAGYLPFGLECLAVADLCLSRKFSGVVAYYGAAEGGTDLAQR
                     KPTVAER"
     gene            complement(616..1560)
                     /locus_tag="PKK81_12380"
     CDS             complement(616..1560)
                     /locus_tag="PKK81_12380"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNV33728.1"
                     /translation="MPARSVLPGMASIPRYVGLAILFLASAWSSPSFPAANRAPSPPP
                     SAELAKHLTSIAKLTTGSPTIRIPEGPFLLGSVRVDDDPYGMGTQFDDTELPQHRVWM
                     DAYEIDRDEISLGEYLSYLFAHKLHPSKDLQHLIWHVITVHSVTDETLAQWPALYVTW
                     KEADSLCRSLGKRLPTEAEWEKAARGPDGNRFPWGDTLPDSTLAMFGQHHVHEIPILA
                     PVSSGEAGKSYYGVHHMAGNVAEWVNDWFGFDYYAYMPERNPPGPTSGRYKSLRGGSW
                     KSRPIMLRTATRSGAPADQRSATVGFRCAKSVPPSAAP"
     gene            complement(1560..2171)
                     /locus_tag="PKK81_12385"
     CDS             complement(1560..2171)
                     /locus_tag="PKK81_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33729.1"
                     /translation="MNSLQDGNKVMQQQIDLLNQELRDAKKQAEHAQAEAKALQAEAK
                     ALGTKLDSQLAESKKLSGEIQRTAAKAAQATDNIRVEEKGSQVEDLPRPLAAVGKAAE
                     EALARNGYRVKVSVKTDQKTIYVTERKVTTPTSLETSGSRNQYVVSLQALPSNVTRLS
                     VKADFERLAQGGRILAVDAEETAEIERRLIAEIGKAVASPSKL"
     gene            2360..3091
                     /locus_tag="PKK81_12390"
     CDS             2360..3091
                     /locus_tag="PKK81_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33730.1"
                     /translation="MSIFIHVPAGLTHHETSGYSVVMAFWCKVVLLVSLALCQPATAL
                     VFAAATPEQPESVPADEYPFYDLAVEAKFLTSRTRLVIIERMTSTRLHPEEPQLPTVA
                     WFAEQGFFDGRLPQDLIRDFVAKAQRPARMDARFTFGVRYRFVSGEGVPETEAAVPVV
                     PAAWPIEENGDEDALETLDRLAFSRVGLTLRGDQALLYVANQRPNATGAGFLFWFVRR
                     QGRWEIYDTDVLWVAQPDQNPSGRR"
     gene            3142..3771
                     /locus_tag="PKK81_12395"
     CDS             3142..3771
                     /locus_tag="PKK81_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV33731.1"
                     /translation="MIRKTFSVPPLGCNCSIIGDPITKQAIVVDPGGAPERILQEVRA
                     MGLTVVSIVHTHAHFDHFLASGAMKQATGAALCLHPDDLPLWDILEMQCRMFGVPYVP
                     APPPDYWLKDEERLAIGELEGIALHTPGHTPGSMCFHFPAAKLVVAGDTLFRGSIGRT
                     DLWGGDFDVIEQSIRERLYTLDEQTAVVTGHGPETEIGRERETNSFVRA"
BASE COUNT          614 a         1039 c         1250 g          887 t
ORIGIN      
        1 cttctggtgg ttcttcgaat atctgaatcg ctttgttcag aactggcact acgtcggtgg
       61 aggagagtta agcgcgtggg agtatctggt gcgcgcgacg ctacccttct ccacggtgct
      121 gcccgcggta cttggtacgg cagaatggtt gtcgtcattt ccgaggttat cggcagggct
      181 ggatcggttt gtgacgttcg aattcaagaa ccatacgacg tgggggtggg ggctcttgat
      241 cggttcggcc gtcgggcttt tcggaatcgg tctgtggcct gactatctct tctcgctggt
      301 gtgggtcgcg ccgctgttgc tgatcacgtc gctgcaattg attcgggatg agccgacgat
      361 ctgcagcggc acggcgcaag gcgattggag aaccctttgg gcagcggcgc ttgcggcgtt
      421 gatctgcggc tggttctggg agatgtggaa cttttacagc ctggcccgct gggaatatgc
      481 ggtgccgttc gtgcaacggt tcaaattgtt tgagatgccg ctgctcgggt atgccggcta
      541 cctgccgttc gggctggaat gtctggctgt ggcggacttg tgcttgtcga gaaagttttc
      601 gggagtcgtg gcctattacg gtgcggctga aggcggcacc gatttggcac aacgaaagcc
      661 cacggtggcg gagcgctgat cggcaggtgc gccgctgcgg gtggcggtcc tgagcatgat
      721 cggccggctt ttccaggacc cgccgcgcag gcttttgtac cggccgctgg tcggtccggg
      781 cggattgcgt tccggcatgt aggcgtaata atcgaagccg aaccagtcgt tgacccattc
      841 ggcgacattc cccgccatgt ggtgaacgcc atagtaactt ttccccgcct cgccgctgct
      901 cacgggcgcc aggatcggga tctcatgcac gtgatgttgc ccgaacatgg ccagtgtact
      961 gtcggggaga gtgtcccccc aggggaagcg atttccgtcc ggcccgcgcg cggctttctc
     1021 ccattccgct tcggtcggca ggcgtttgcc caatgagcgg cagaggctat cggcttcttt
     1081 ccaggtcacg tagagcgccg gccactgggc cagcgtctca tcggtaacgg agtgcactgt
     1141 gatcacgtgc cagatgagat gttggagatc tttcgacggg tggagcttgt gggcgaatag
     1201 ataggacaag tattccccga gactgatttc gtcgcggtcg atttcgtagg cgtccatcca
     1261 gacgcggtgc tgcgggagtt ccgtatcgtc gaactgtgtg cccatcccat agggatcgtc
     1321 atccacccgg acgctgccca gcaggaacgg cccttcggga attcgaatgg taggcgaacc
     1381 ggtcgtgagt ttggcgattg atgtcaggtg tttggccagt tcagcagaag gtggaggact
     1441 gggggctcgg ttggcagcgg ggaaactcgg gctggaccag gctgaggcaa ggaacaggat
     1501 tgcgaggcct acgtagcgtg gaatggaagc cattcccggt aagacggaac gggcaggcat
     1561 tacagtttac tcggcgaggc gacggctttt ccgatttcgg caatcagccg gcgttcgatc
     1621 tccgcggttt cctccgcatc caccgccagg atgcgaccgc cctgcgccag gcgttcaaag
     1681 tcggctttga cgctcaaccg ggtgacgttg gacggcaggg cttggagcga caccacatac
     1741 tgattgcgcg agcctgaggt ttccagcgag gtcggggtgg tgactttgcg ttcggtgacg
     1801 tagatcgttt tctggtcggt cttgacgctg actttcacgc ggtagccgtt gcgcgccagg
     1861 gcttcttcgg cggcttttcc gactgcagcc agcgggcgcg ggaggtcctc gacctgggag
     1921 cccttctctt cgacgcggat attgtccgtg gcctgggccg cttttgcagc cgttcgctga
     1981 atctctcccg acagcttttt cgactcggcc agttgggagt cgagtttggt cccgagcgcc
     2041 ttggcttcgg cctgcagcgc ctttgcctcg gcttgagcat gctccgcctg cttcttggcg
     2101 tcacgcaatt cctggttcag cagatcgatc tgctgctgca tgaccttgtt gccgtcctgc
     2161 agcgaattca tgaccgactc ctgcttcgcc agttgcttgc ggaggctttc gttttcttgg
     2221 cgaaacggcg actcgtccgg tgcgcatccg acacaccacg atagtactgc gaggctgaag
     2281 agagctgcga gcgatcgctg gggaggcttg atcatagttt gggtaagacc atcagaccaa
     2341 taccagagtt tggcccccct tgtccatctt cattcatgtt cccgccggtt tgacccatca
     2401 cgaaacaagc ggctattctg tggtcatggc gttctggtgc aaggtggttt tgctcgtcag
     2461 tctggcgctg tgtcaaccag cgacagcctt ggtgtttgcc gcggcaacgc ccgagcagcc
     2521 ggagtccgtg cctgccgacg agtatccgtt ttatgacctc gccgtcgagg caaagttcct
     2581 gacctctcgt accagactgg tcatcatcga acgcatgacg tccacccgtc tgcatccgga
     2641 ggagccgcag ttgccgacgg tggcctggtt tgccgagcag ggatttttcg atggccggtt
     2701 gcctcaggat ctcattcggg atttcgtggc gaaggctcag cggccggctc gaatggatgc
     2761 ccgttttacc ttcggggtgc gctaccggtt cgtgtccggc gaaggggtgc cggagactga
     2821 agctgctgtc ccggttgttc ccgctgcttg gcctattgag gagaatggtg acgaggatgc
     2881 tctcgagacc ctcgaccggc tggccttctc ccgtgtggga ctgacattgc ggggcgacca
     2941 ggccttgctc tatgtggcga atcagcggcc taatgcgacc ggtgccggat ttttgttctg
     3001 gttcgtgcgc cgacaggggc gttgggagat ttacgatacg gacgtgttgt gggttgcgca
     3061 gccggaccag aatccttcag gccgtcgata gcaggggggc aggtgcgtcg tggtactcgt
     3121 ttggttcact gaggagtgtg catgattcgc aagacctttt ccgttccgcc gctcggttgt
     3181 aactgctcga tcatcggaga tccgatcacg aaacaagcga ttgtggtcga tccgggcggg
     3241 gcgcccgagc gcattctgca ggaagtccgc gcgatggggt tgacggtggt gagcattgtg
     3301 cacacccatg cgcatttcga tcactttctc gcatcggggg cgatgaagca ggcgaccggc
     3361 gccgcgctct gcctgcatcc cgatgatctt cctctgtggg acattctgga gatgcagtgc
     3421 cgcatgttcg gcgtgcctta tgtcccggcg ccaccgccgg actattggtt gaaggatgag
     3481 gagcggttgg cgatcggcga gttggaaggg atcgcgttgc acacgcccgg gcataccccc
     3541 ggttccatgt gttttcactt tcctgctgcg aagctggtgg tggccggtga caccttgttt
     3601 cgcggatcca tcggccgcac cgatctctgg ggcggagatt ttgatgtcat cgaacagtcg
     3661 atccgggaac gactgtatac gctcgacgaa cagactgccg tggtcaccgg gcacgggccc
     3721 gagacggaga ttggccggga gcgggagacc aattcctttg tgcgagccta gcaggatgcg
     3781 gaaaaagtcc
//
LOCUS       DAOQRM010000213         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_26874_length_3755_cov_15.749459, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000213 DAOQRM010000000
VERSION     DAOQRM010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26874_length_3755_cov_15.749459"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKK81_12400"
     CDS             complement(<1..235)
                     /locus_tag="PKK81_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNV33732.1"
                     /translation="MKFFFYADNLNPSQLKRRAPEHKFLFTAYLPDHSIQFCRWSTQW
                     RCGLASVIPSPGEKVWGAVFEITDEDLKILDEFE"
     gene            426..881
                     /locus_tag="PKK81_12405"
     CDS             426..881
                     /locus_tag="PKK81_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33733.1"
                     /translation="MSKVIHYTVLALLLAGGLAYWWMKPPGLNPMLDSQAAEALALVQ
                     THRAAGYPTILQAFNERARIQEARKLGLRLGEWKVIKQDGQRYEVRIYMREQGTTQWF
                     ERDFIWHVDLATKRVNAASLPADGLMPEGPESGRPRAPGGEVPSFPPTM"
     gene            complement(871..1197)
                     /locus_tag="PKK81_12410"
     CDS             complement(871..1197)
                     /locus_tag="PKK81_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNV33734.1"
                     /translation="MDSKPDYITVAQVDQVPPGTCRTVEVEGIFLALCNVDGTFHVVD
                     NTCPHAGGPLGEGCMDGELVECPWHGWRFNVRTGERPENPEIRVACVEVRVQGTNVQV
                     KTPLTL"
     gene            1396..1941
                     /locus_tag="PKK81_12415"
     CDS             1396..1941
                     /locus_tag="PKK81_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNV33735.1"
                     /translation="MEMTLLLNSTYEPLRVLHWQKAITLLWQGKVEVLEVYDRQIHGI
                     SISIKLPSVMRLLKLVKLKDSHRAVKFSRINIFTRDGYCCQYCKHKFRTEELTFDHVV
                     PIAKGGRKTWENIVTACWRCNNRKSGRTPEEANMRLIKRPVKPRWSPTVTITIGIRNT
                     PESWRDYLYWNLELDADPAET"
     gene            complement(2070..2522)
                     /locus_tag="PKK81_12420"
     CDS             complement(2070..2522)
                     /locus_tag="PKK81_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33736.1"
                     /translation="MAGYTGRSLAMAGGIIGIAAVAAYFGIAHIGEPDQGGGSIAGQV
                     QIAPNLADQVKPTDVLFVIVRRPTGPPRPIAAKRIDAPKFPVQFEITSADVMVQGTEL
                     KGMVDVIARLDRDGQAGQAQPGDMEGRYPKNPTLPGGKDVNITIDKVY"
     gene            2697..3350
                     /locus_tag="PKK81_12425"
     CDS             2697..3350
                     /locus_tag="PKK81_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNV33737.1"
                     /translation="MAANPGFQISLDVRGWPVLVIGGDEEAADKVQRLLEAGAKVTVI
                     SPTLHEVLRKLAASAKIIHRGRHFRANDLEAVILVLNTIRDDRELSHSLMRLAREQKF
                     LIWSVDQPDVSNVVMPAVVSSGHVRVAISSSGQAPALSGFMKEDLERILDNEFVAFVD
                     WLGQLREQAKANEPDAEKRRALLREALDGFRLLGKVQYPKVWLDQRAAVGAGEKRGS"
     gene            3400..3708
                     /locus_tag="PKK81_12430"
     CDS             3400..3708
                     /locus_tag="PKK81_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNV33738.1"
                     /translation="MVLLEFSMSPLGKGESVSKYVSRSLDIIDKSGVDYRLNPMGTVL
                     EGEWDEVMAVVKKCYMRMRKDCNRVSCSIKIDYRKGPKGRLSSKVSSVESQLKRKLKV
                     "
BASE COUNT          786 a         1045 c         1130 g          794 t
ORIGIN      
        1 cttcgaattc gtcgaggatc ttgagatctt cgtcggtgat ttcgaagacg gctccccaga
       61 ctttttcccc cggcgacgga atcacgctcg ccaggccgca tcgccattgc gtcgaccagc
      121 ggcagaactg aatgctatgg tcggggaggt acgccgtgaa aagaaacttg tgttccgggg
      181 cgcgacgctt gagttgagac gggttcaaat tgtccgcgta aaagaagaac ttcattgaac
      241 agtgagactc cgattatgtc gcgaaggttg accggtactt gtaccatagc gttgcgcgac
      301 cttgtcaagc gcgggggctc ctgcgcgtcg ccggtttcaa cgcatgcgac gattgacagt
      361 gaattcacac gccacctatg atgacgagct gatcaatcat ccaccccagt ttattgtgac
      421 gacctatgag taaagtgatc cattacacgg tgcttgcgct tctcctggcc ggagggttgg
      481 cctattggtg gatgaaacca cccggcctca acccgatgct cgactcgcag gcggccgagg
      541 ccctcgcgct ggtgcaaacc catcgggccg ccggttatcc gacgattctt caggcgttca
      601 acgagcgggc ccgcattcag gaagcccgca agttgggctt gcgcttggga gagtggaaag
      661 tgatcaagca ggacggtcag cgctatgagg tccggatcta catgcgcgag caggggacga
      721 cccagtggtt cgaacgggat ttcatctggc acgtggatct ggcgacgaag cgcgtcaacg
      781 cggcctcgtt gccggccgac gggttgatgc cggaaggccc ggagagtgga cgaccgcgcg
      841 caccgggcgg cgaagtccct tcgttcccgc ctacaatgta aggggcgttt tcacctgcac
      901 gtttgtgcct tgcaccctca cttccacgca agccacgcgg atctccggat tttcaggccg
      961 ctcaccggta cggacgttga atcgccaccc gtgccagggg cattcgacca gttcaccatc
     1021 catgcatcct tcgccgagcg gtccccctgc gtgcgggcat gtattatcga cgacatgaaa
     1081 ggttccgtcg acattacaga gggcgagaaa aattccctcg acttcaacgg tgcggcaggt
     1141 gccaggagga acctggtcca cctgggcgac ggtgatatag tcgggcttcg aatccataag
     1201 cgtcctctgc aagttacagc ggggtacagg tcgtctcaat attgcgtgac atcgtgagca
     1261 gtattgtcta agcgcttgat ttcattggcc gcatatggca tagattgagc aggcattttt
     1321 cagggaaggg tcacaacagc gccgtcattt gctatcctgt aaatagtgca ctcataggag
     1381 atcgccggag ccactatgga aatgaccttg ttgctcaact ccacctatga accgctccgt
     1441 gtcctgcatt ggcaaaaggc cattactctg ctctggcagg ggaaggtcga agtcctcgaa
     1501 gtctacgatc gccaaatcca cggtatctcg atctcgatca agctcccctc ggtgatgcgc
     1561 ctcctgaagc tggtgaagct caaggacagc catcgcgcgg tgaagttttc ccgcatcaac
     1621 atttttacgc gcgacggcta ctgttgccag tactgcaaac acaagttccg caccgaggaa
     1681 ctgacgttcg accatgtcgt gccgatcgcg aagggtggtc ggaagacctg ggagaacatc
     1741 gtgacggcct gctggcggtg caataaccgg aagagcggcc ggaccccgga agaagccaac
     1801 atgcgtttga tcaagaggcc cgtaaagccg cgctggagtc ccaccgtcac gattaccatc
     1861 gggatccgga acacgccgga aagttggcgg gactatctgt actggaacct cgagctggac
     1921 gcggacccgg ctgaaaccta gccggctgtt gaaacgggtt tttggcattg cactcgcggt
     1981 gctcaccact tcaaggtaca agccaagtgc gcccggcctc tcgctggttg cagccttacc
     2041 gaatcaccgg ttcagacagc ctgccgcagc taatacacct tgtcgatggt aatgttcacg
     2101 tcctttcctc ccggtaacgt cggattcttc ggatagcgtc cctccatgtc acccggttgc
     2161 gcctggccgg cctggccatc tcgatccaga cgcgcaatca catccaccat gcctttcaat
     2221 tcggtgccct ggaccatgac atcggcactc gtaatctcaa actgcaccgg aaatttcggc
     2281 gcgtcgatcc gtttggccgc gatgggtctc ggtggtccgg tcggacgccg cacgatgacg
     2341 aacagcacat cggtcggttt gacctggtcg gccaggttgg gcgcgatctg cacttgcccg
     2401 gcaatcgatc cgccgccttg atccggctcc ccgatgtggg cgatgccgaa ataggccgcg
     2461 acggccgcaa tgccgatgat ccctccggcc atcgccaggg atcgtcctgt atatccagcc
     2521 acaatagact cctttgttga ctcgcggtgc gcgcgacggg ctcgcccgtc atccagcggg
     2581 cgcattatac ccagctcctg ccagaaaggg gaatggcgga gcgggtttac cctgtcaaat
     2641 cggctgttgt atgatacgcg ttcacgtcgt tgtcgatttg ttggaggatg aactcaatgg
     2701 ctgcaaatcc cgggtttcaa atttcactcg atgtgcgtgg gtggccggtc ctggtgatcg
     2761 gtggtgatga ggaagcggca gataaagtgc agcggctcct agaggcaggc gcgaaggtca
     2821 cggtgatcag tcccaccctg catgaagtgc ttcgtaaact ggcggcctcg gcgaaaatca
     2881 ttcatcgtgg gcggcatttc cgcgcgaacg atctggaggc ggtcatcctg gtcttgaaca
     2941 cgattcgcga tgatcgtgaa ctctcccact cactcatgcg attggctcgc gaacagaaat
     3001 ttttgatctg gtccgtcgac cagcccgacg tgtcgaacgt ggtgatgccg gccgtcgtca
     3061 gctccggtca tgttcgtgtg gcgatcagct cgagcgggca ggcgccggcg ctttccggat
     3121 tcatgaagga agatctcgag cgtattttgg acaacgagtt tgtcgcgttc gtcgattggc
     3181 tcgggcagct tcgcgagcag gccaaggcca acgaaccgga cgcggagaaa cgtcgcgcgc
     3241 tgttgcgtga ggcgctggat ggattccgac ttctgggaaa agtgcagtac ccgaaggtgt
     3301 ggctggatca acgggcggcc gttggggcag gggaaaagcg cggatcgtag gaccggtcgt
     3361 tcgttatcgg ccgacagtta tcagcaggag attcccacta tggtgttgct ggaattcagt
     3421 atgtcgcccc tgggcaaggg agagagcgtg agcaaatacg tgtcacgctc gttggatatt
     3481 atcgacaaga gcggtgtgga ctatcggctg aatccgatgg gcacggtact cgaaggggaa
     3541 tgggacgagg tcatggcggt ggtcaagaag tgctacatgc gcatgcgcaa agactgcaat
     3601 cgcgtgtcct gcagcatcaa gatcgactac cgcaagggac cgaaggggcg cttgtcgagc
     3661 aaagtgtcga gtgtggaatc acagctgaaa cgaaagctga aggtctaaca ggctgcggaa
     3721 aaactcgatt gttgcgcagt acgctacaat taact
//
LOCUS       DAOQRM010000214         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_27497_length_3700_cov_15.000823, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000214 DAOQRM010000000
VERSION     DAOQRM010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27497_length_3700_cov_15.000823"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..948
                     /locus_tag="PKK81_12435"
     CDS             64..948
                     /locus_tag="PKK81_12435"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV33739.1"
                     /translation="MLNWFSQSPQSDSVVITGASTGIGAACALALDKLGYRVFAGVRH
                     SADGERLQQQAGPRLMPIRLDVTDAASISAASHTVAAMVGERGLAGLVNNAGIGVAGP
                     IELLPLADWRRQFEVNVFGLIAVTQTFLPLIRTGRGRIINMGSIAGRASMPFMAPYAA
                     SKHALEAITDALRLELQPWGIRVALIAPGAIATPIWKKTRKEVDAWDAAWGQDLKGMY
                     REGFTRIKDAATAAGEQAQPASVVAETVAHALRSRWPKTRYLVGSDAAIRAYLALLLP
                     DRLNDWLLTRIVKLPPRR"
     gene            complement(955..1728)
                     /locus_tag="PKK81_12440"
     CDS             complement(955..1728)
                     /locus_tag="PKK81_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33740.1"
                     /translation="MRLGVLIGAIMLVGWVAGCGSSAKLMSGLSIEELKPVPGILPSA
                     RLIRGTISSFSGDRVEVETGERAPRYLSLEAGGDEARGLQPGGAVEIMVNDRDEIVAY
                     QRPADQPPTKVFRGSLGRSFNPQKERVTIRLDTGRDVGFYARRVAMEKLAALEVGETA
                     DFAVDRAYLLVDVFSLGKPRELALESSSRAPHHRLQGAFVTIGDGRMRLRMKIDDIQS
                     FPVRPLLQPAIEQLAPNELVSVYLDAQGQVIDMVRATRR"
     gene            1920..>2110
                     /locus_tag="PKK81_12445"
     CDS             1920..>2110
                     /locus_tag="PKK81_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33741.1"
                     /translation="MTPPPIETAAVDSFETTLEGGTSQIGLFLILILLTIGGIYVAGE
                     LTSDLSDVRASSVWPFILLG"
     assembly_gap    2111..2210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2211..3392
                     /locus_tag="PKK81_12450"
     CDS             <2211..3392
                     /locus_tag="PKK81_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNV33742.1"
                     /translation="MVFALLIAAILWNLGTWHLGLPSSSSHALIGSVIGVGITNQLMA
                     AGGTNTSGVDWTQALNVGKSLLLSPLIGFSAAAFLLLIAMKAVPVEKLYKPPRGKTPP
                     PLWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTVYALNRTVDISYTPAFV
                     EASSKSEAVFQRYSKATTTPADPRQIVAEYLRTHEVRPDTLPALRALSGIVRTQIASY
                     ATVAQIPTEQTSNVRNDMYLLGESLRWMDKTREPAFARADRDILLHYKSMLDNATKYI
                     PVWVKAAVAIALGLGTMIGWKRIVVTVGEKIGKTHLSYGQGASAELVAMTTIGAADLF
                     GLPVSTTQVLSSGVAGTMAANRSGLQWQTVRHLALAWVLTLPASIVLAGTLFWFFRHF
                     A"
BASE COUNT          692 a         1188 c         1031 g          689 t
ORIGIN      
        1 gagtagggtc gcgccgtaaa ttatgatagg gtacgcgacc ggccggaaag aaccggcctc
       61 gatatgctga attggttttc acaatcgccg caatccgact ccgtcgtcat caccggcgcc
      121 tcgaccggca tcggggccgc gtgtgcgttg gcgctcgaca agctggggta ccgtgtcttc
      181 gcaggcgttc gtcattccgc tgacggagaa cggctccagc aacaagccgg accgcgactc
      241 atgccgattc gcctcgatgt caccgatgcg gcctcgatct cggcagccag tcataccgtg
      301 gcagccatgg ttggagaacg cggcctcgcc ggtctggtga acaatgccgg aatcggcgtc
      361 gcggggccga tcgaactcct gcccctcgcc gactggcggc ggcaattcga agtcaacgtg
      421 ttcggcctca tcgcggtcac gcaaacgttc ctcccattga tccgcaccgg gcgaggacgc
      481 atcatcaata tgggttccat cgccggacgg gcctccatgc cgttcatggc cccctacgcc
      541 gcgtcgaaac atgcgctgga agcgatcacc gatgccttgc gcctggaact ccaaccctgg
      601 ggcatccgcg tcgcactcat cgcgcccggc gccatcgcca cgcccatttg gaaaaagacc
      661 agaaaagaag tcgatgcctg ggacgcagcc tgggggcagg acctcaaggg catgtaccgg
      721 gaaggcttca ctcgcatcaa ggacgcggcc actgcggccg gtgaacaggc acaaccggcg
      781 agcgtcgtgg ccgaaaccgt ggcgcatgcc ttgcgttcga ggtggccgaa aacacgctat
      841 ctcgtcggct cggacgccgc catccgtgcc tacctggcgc tgctcctgcc ggatcgcctg
      901 aatgactggc tgctcacccg gatcgtgaag cttcccccgc gccgctgacg tcgttcaacg
      961 ccgggtcgct ctgaccatat cgatcacctg cccctgcgca tcgagataga ccgataccag
     1021 ttcgttggga gcgagctgtt caatcgcggg ctgcagcaac ggccgaacgg gaaaggactg
     1081 aatgtcatcg atcttcatcc tgaggcgcat ccgtccgtca ccgatcgtca cgaatgcgcc
     1141 ttgcaggcga tggtgcggcg cgcgtgacga agactcaagg gccagttcac ggggcttgcc
     1201 caacgaaaac acatcgacga ggagatacgc tcgatcgacg gcaaaatcgg ccgtctcgcc
     1261 gacctcaaga gccgccagtt tctccatggc gaccctgcgc gcatagaacc ccacatcccg
     1321 ccccgtatcg aggcgaatcg tcactcgttc cttctgaggg ttgaacgatc tccccagcga
     1381 gccgcggaag actttcgtgg gcggctgatc ggcaggtcgc tgataggcca caatctcgtc
     1441 gcgatcgttg accatgatct ccacggcccc acccggctgc agtccacgcg cctcgtcccc
     1501 gccggcttcg agtgacaggt agcgcggagc ccgttccccg gtctcaacct ccacacgatc
     1561 gccggagaaa ctgctgatcg tgcccctgat caacctcgcg ctgggcagga tgcccggcac
     1621 cggcttcaac tcctcgatac tgagcccgga catgagtttt gccgagcttc cgcaaccggc
     1681 aacccagcca accagcatga tcgcaccgat caaaactccc agcctcatgt gcgtcgcgtc
     1741 cattgtccct ccggctttct tccgtcggat gataaaacaa acccctgccc ggaggcgagc
     1801 gtccgtcttc cacagttaac ctgatcttaa tccggctcac agcgaggtgt aacgacatcc
     1861 tgccagactc tcaggccgtt aacggttcgg cagcggctct ccgcagaaag gaaccctgaa
     1921 tgacaccgcc ccccatcgaa accgccgctg tcgattcctt cgagaccact ctcgaaggag
     1981 gaacttcgca aatcggcctc ttcctcatcc tgatcctgct cacgatcggc ggaatctacg
     2041 tcgccggtga attgacctcg gatctctccg atgttcgcgc ctcctcagtc tggccgttta
     2101 tcctgctcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atggtgtttg
     2221 cgctgctgat cgccgccatt ctctggaacc tggggacctg gcatttgggt ctgccttctt
     2281 ccagttcgca tgcactgatc ggctccgtga tcggcgtcgg aatcaccaac cagctgatgg
     2341 cagccggcgg gacgaatacc agcggcgtcg attggacaca ggccctcaac gtgggcaaat
     2401 cccttctgct ttcgccgctc atcggattct ccgccgccgc gttccttctc ctgatcgcca
     2461 tgaaggccgt ccccgtcgag aagttataca aaccgccgcg cggcaaaacg ccgccgcccc
     2521 tgtggatccg gggtctgctc attctcacct gcacgggtgt gagctttgcc cacgggtcca
     2581 acgacggaca aaagggcatg ggtctcatca tgctgatcct catcggaacc gtgccgacgg
     2641 tctatgcgct caatcgcacc gtggacattt catacacacc tgccttcgtg gaagcgtcga
     2701 gtaaatcgga agccgtgttc cagcgctaca gcaaggcgac caccactccg gccgacccgc
     2761 ggcaaatcgt cgcagaatac cttcgcaccc acgaggttcg cccggacacc ttgccggctc
     2821 ttcgcgcatt aagcggcata gtccgcacac agattgcctc ctatgccacc gttgcccaga
     2881 tcccgacgga acagacctcc aacgtgcgaa acgacatgta cctgttgggc gaatccctgc
     2941 gttggatgga caaaacccgt gagcctgcct ttgcgcgagc ggaccgagac atcttgctcc
     3001 actataaatc catgttggat aacgccacca agtacatccc cgtgtgggtc aaagccgccg
     3061 tggccatcgc gctggggctg ggcacgatga tcggctggaa acgcattgtg gttacggtcg
     3121 gagagaagat cgggaaaacg catctgtcgt acggccaggg cgccagcgcg gaattggtcg
     3181 cgatgacgac catcggcgcg gcagatctct tcgggctgcc ggtcagcacg acgcaggtgt
     3241 tgtcctccgg cgtggcggga accatggccg ctaatcggtc gggattacag tggcagaccg
     3301 tccggcatct ggcattggca tgggtcctga ctctccctgc gtccatcgta ctagcaggaa
     3361 ccctattttg gttttttcgc cattttgcat gacccgcgcg gcggtttatg ggccaaacat
     3421 tcggcacagg cactcatcga ggaagccggc gccaaccggc cgttcggtct tcgactgccg
     3481 agggatcgat caggaagcgg gaagcgcctt cagcaatcgt tctgcttccg gccggaatct
     3541 gcgcctccag gcgtagggat aatgtggatg ttcgacctga agcacggcgt cgagatgcgc
     3601 gcgcgcttcg ccggatcggc cctgtttgat caggagtcgg gccaggatga gatgggcatt
     3661 ggtataacgt ccgtccgccg caatcgcgcg ccgcagatac
//
LOCUS       DAOQRM010000215         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_27553_length_3693_cov_14.932380, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000215 DAOQRM010000000
VERSION     DAOQRM010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27553_length_3693_cov_14.932380"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKK81_12455"
     CDS             complement(<1..391)
                     /locus_tag="PKK81_12455"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNV33743.1"
                     /translation="MTLRVSLIVLFSIGGILLLVGYWAGQYHPDLLPHTFSLQTAPPD
                     PSQGQPGVTINLPPVVAPVDDGQRYYYVQVNLALELDRSGTAGLIQARHDAIDRQVME
                     ILHTYAVSDLRATGQLPALRADIRRAIN"
     gene            complement(748..1164)
                     /locus_tag="PKK81_12460"
     CDS             complement(748..1164)
                     /locus_tag="PKK81_12460"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HNV33744.1"
                     /translation="MAVYPLTVFYDGACPICAREMALMKRLDRTQRLTLLDFSLAGFD
                     AAPAGLAVADLSAVIHAQWADGAVITGVEVFRAIWEAVGLGFLSRLSRLPMVAPLMTK
                     AYGWFARNRLWLTGRNNACPGNACAAASPKPLRASQ"
     gene            complement(1183..2100)
                     /locus_tag="PKK81_12465"
     CDS             complement(1183..2100)
                     /locus_tag="PKK81_12465"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNV33745.1"
                     /translation="MNTHRIHRVAKLTGLSRDVIRVWERRFGLLKPTRGANRYRNYSD
                     EDVALLRYLKQQLDAGASIGDLAKLGREELITRLRAEAPRTAVVDNTFDRLLRELLSA
                     LEPFDRVTFEKRLNGAVAVVPFEEALHGILLPLQERVGDLWHSGRISIALEHYVTSQI
                     EQKLYAAMNQLPVAEFGAKVVVACPPGEEHDIAALAVAYRCRVRGCRVYYLGANVPIS
                     SLGRLCREVAPDLAILSLTLVLQEGNAAELIHSLAEELKPVTTVMAGGHGARVMRDQL
                     AQYRIDVLDTFSELDEVLDRFTRRFTIPG"
     gene            2238..2534
                     /locus_tag="PKK81_12470"
     CDS             2238..2534
                     /locus_tag="PKK81_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33746.1"
                     /translation="MATTKTMEGTVPVSEIRRQILTLYQESNCLRCGGLMVRDFCTDL
                     LSGASGLDSSTSRCVQCGDVVDPVIRWNRHLQQAAGVVREMKTSKPALQGQVSV"
     gene            2592..3554
                     /locus_tag="PKK81_12475"
     CDS             2592..3554
                     /locus_tag="PKK81_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNV33747.1"
                     /translation="MRRHIGLQIGIGAVLVAGSMSMAVSAKAAGDHKDMVLIPSGEFT
                     MGSHEHSDEARHQVVLDAYLIDKFEASNTRYKEFMRATGHPAPAYWDDPRLSKPEQPV
                     VGVSWTDANAFCKWDGKRLPTEAEWEKAAKGPEGDNHYPWGHHLDPKKANYGQNVGRT
                     TPVDSYPEGVSGYGVYNMAGNVFEWVDDWYDPKYYRTSNALNPRGAEKGYNFANQGPV
                     KVLRGGSWLAPETSLHTSHRFWNQPENNSYGVGLGFRCAKSASMVSDEAAQAGRDAFI
                     QALVGMGAEKNADALASIEKALAADPDNKEYLATRDLIKKSMKK"
BASE COUNT          794 a         1032 c         1160 g          707 t
ORIGIN      
        1 tgttgatggc gcgtcgaatg tctgctcgca aggccggcag ctgcccggtc gcacgcagat
       61 cgctgacggc ataggtgtga agaatttcca tcacctgccg atcgatcgcg tcgtggcggg
      121 cctggatcaa tccggcggtc cccgagcgat cgagctccaa ggccagattg acctgcacat
      181 aatagtaacg ttggccgtca tccacgggcg cgacgacggg cggcaggttg atggtcacac
      241 ctggttgtcc ctgtgaggga tcggggggag cggtttgcaa tgagaacgta tgcggcagga
      301 ggtccgggtg gtactggccc gcccaatagc ctaccaggag taggataccg ccaatgctga
      361 acagcacgat gaggctgacg cgtaatgtca tgctgaaggg gatgaatgtg acgtgaagcg
      421 gcggtcctgt tcccgatgac actgctgacg cggtctcgca ccatgaggac gttctgaata
      481 accgtagcat ctccttgaaa attgtcagca agggacttcc cgggtagaca ctaggaaatt
      541 gcccaggcct ggtggttcgt gatggttagt gccgttggtt gaggttcgga tggttgcgta
      601 gggcgcctct gtcagacttt tccttcgcac gatgccatgt tcgaccgagt gggaacatgc
      661 ccgggctcgg aatgaaggcc tgatccgggc ctcgtggctt cgccgtgctt tgtcatgact
      721 ggcgcgttgg gaaagagagg acggggacta ttgtgaggct cgcagtggct tgggtgaagc
      781 ggcggcgcaa gcatttcccg gacaggcgtt gttgcggccg gtgagccaga gccggtttct
      841 ggcaaaccat ccataggcct tcgtcataag tggagcaacc atcgggagtc ggctgaggcg
      901 ggacaggaat cccaggccga ccgcctccca aatcgcacgg aagacctcga caccggtgat
      961 gacggcgccg tccgcccact gggcgtgaat caccgcgctc agatccgcga cagccaagcc
     1021 ggccggtgcg gcatcgaagc cggcaagaga aaagtcgagt agggtgaggc gttgtgtcct
     1081 gtccagacgc ttcatcagcg ccatttcgcg ggcgcaaatg gggcaggctc cgtcatagaa
     1141 cacggtgagt ggatagacag ccatccgaac ggtgcctccc tgtcatcccg ggatcgtaaa
     1201 tcgcctggtg aatcgatcca gcacttcgtc caactcgctg aaggtatcca ggacatcgat
     1261 gcggtattgc gcaagctgat cgcgcatcac gcgcgcgcca tggccgccgg ccatgaccgt
     1321 ggtaacgggt ttcaattcct ctgcgagcga gtgaatcaat tccgctgcgt tgccttcctg
     1381 cagcaccagg gtcaacgaca ggatggccag atccggggcg acctccctgc agagcctgcc
     1441 gagggaggag atcggcacgt tggctcctaa gtagtagacc cggcatccac gcacgcggca
     1501 acggtaggcg actgccaatg ccgcaatgtc gtgctcctca ccgggcgggc aggccacgac
     1561 gactttcgcg ccgaattccg ccaccggcaa ttgattcatg gccgcataca gtttttgttc
     1621 aatttggctg gtcacgtaat gttccagggc gatactgatg cgcccgctgt gccacaggtc
     1681 tccgacgcgt tcctgcagcg ggagcagaat tccgtggagt gcctcttcaa acgggacgac
     1741 tgcgaccgcg ccgttcaagc gtttctcgaa tgtgacgcgg tcgaatggtt ccaaggccga
     1801 gagcagttcc cgcaagagtc ggtcgaacgt attatccacc acggcggtgc gcggcgcttc
     1861 cgcgcgcagg cgggtgatca actcctcacg ccccagcttg gcgagatcac cgattgaagc
     1921 tcccgcatcc agttgctgct tcagatagcg cagcaaggcc acatcttcat ccgaataatt
     1981 gcgatagcga ttcgcgcctc gtgtcggttt cagcaggcca aatcggcgct cccacacccg
     2041 aatcacatcg cgactcagac cggtgagctt cgcaacccta tgaattctgt gcgtattcat
     2101 tgttgagccc aagtatatgg ttgtgtccaa tgtttgtcaa atatttattg aaataactat
     2161 tgacacattg gacaaaaaag gttaaacatt ggacatagag gttgcaaaca ttggacatga
     2221 acatgcgagg agtgatcatg gcgaccacaa agacaatgga aggaactgtg ccagtaagtg
     2281 aaatccgaag acagattctg actctttacc aggagtcgaa ctgcctgcgg tgcggcggac
     2341 tgatggttcg tgatttctgt acggacctgc tcagcggggc aagcgggctc gattcctcca
     2401 cttcgcgttg tgtgcaatgc ggtgatgtcg ttgatccggt catccgatgg aaccggcacc
     2461 tgcagcaggc cgccggcgta gtacgggaaa tgaaaacatc gaagccggcc ctgcagggcc
     2521 aggtctctgt ttagcaacaa tcgtgacccc actgggggtt gtgtcagggt tctcaaccaa
     2581 ggaggtttct catgcggagg cacataggtc ttcaaattgg aataggagcg gtgcttgtag
     2641 ctggatccat gtcgatggcg gtgtcggcga aggccgccgg cgaccataag gacatggtcc
     2701 tcatcccgag cggcgagttc accatgggca gtcacgaaca ttccgacgag gcgaggcatc
     2761 aggtagtgct cgatgcctat ctgatcgaca agttcgaagc gtcgaacacg cggtacaagg
     2821 agttcatgcg ggccaccggt catccggcac cggcctattg ggacgatccg cggctcagca
     2881 agcccgagca accggtagtc ggagtgagtt ggacggacgc caacgccttt tgcaagtggg
     2941 acggcaagcg attgcccacc gaggcggaat gggaaaaggc cgccaagggc ccggagggag
     3001 acaaccacta tccgtggggg catcacctcg atccgaagaa ggccaactat ggccagaatg
     3061 tgggacgcac tacgccggtg gactcctatc ctgaaggcgt gagcggttat ggcgtgtaca
     3121 acatggccgg caacgtcttc gagtgggtgg atgactggta cgacccgaaa tattaccgca
     3181 ccagcaacgc gctcaatccc cgtggcgccg agaagggcta caacttcgcc aatcaaggcc
     3241 cggtgaaggt cttgcgcggc gggtcctggc tggctccgga aacctcgctg cacaccagcc
     3301 accggttctg gaatcagccc gaaaacaact cctacggcgt cggactgggt ttccgttgcg
     3361 cgaagtcggc cagcatggtc tccgacgaag ccgctcaagc cgggcgtgat gccttcattc
     3421 aggcgctcgt cggcatggga gcggagaaga acgccgacgc cctcgcctcc atcgagaagg
     3481 cgttggcggc ggatcccgac aacaaagagt atctggccac tcgcgatctc atcaaaaaga
     3541 gcatgaagaa gtagcacata accgtggagg gggcgggtga ccgtcccccg gcccggccct
     3601 caggggcagg aggtgtggca tgaagacaat ggggaacgca gtcggggcag ggctcatcat
     3661 gactgtcgcc atcatgacgg cgacggcaca ggc
//
LOCUS       DAOQRM010000216         3646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_28088_length_3646_cov_16.217488, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000216 DAOQRM010000000
VERSION     DAOQRM010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28088_length_3646_cov_16.217488"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..409
                     /locus_tag="PKK81_12480"
     CDS             215..409
                     /locus_tag="PKK81_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV33748.1"
                     /translation="MNKQLLRVGEAADVLAVSRWTIYRWVEEGRLEGTKIGRGSLRVF
                     RTSLDRLVQNNKTQEMNLTV"
     gene            483..>1582
                     /locus_tag="PKK81_12485"
     CDS             483..>1582
                     /locus_tag="PKK81_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33749.1"
                     /translation="MHRILLCLVFLLAGCAGGTGLSSSSKASQQTGPSGSDSYAFTNG
                     RWFDGKGFQAATWYSVQGRLTRTPPQGKVETVDLSGLFVVPPFGEAHNHNVEGPWNVR
                     AVAERYLKDGVFYVKNPNNVRDLALETRGAVNRPTSIDATFAHAGLTGQGGHPIALYE
                     DVLRLGRYEPAIGTVERGWFENRSYIVLDTDADLETKWPLITSGRPDFLKVYLVHSED
                     EDATGKSGHSPQRSGLHPRLVAQIVAKAHAAGLQVTAHVETAADFRHAIRAGVDEIAH
                     VPGWLVNSAADAESARLTEADARLALERHVRVVTTAVAGSAMPSVTGHHPHGHYEGHA
                     TGPSDHHATAPDESLSQVLKDNIALLHRVGVRV"
     assembly_gap    1583..1682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1683..3470
                     /locus_tag="PKK81_12490"
     CDS             <1683..3470
                     /locus_tag="PKK81_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV33750.1"
                     /translation="YKGANALRYGANSIGGAINFVPRTGYSASTLQMRMLGGSFGMVS
                     GQVSSGKVLQPFKVGNMSATMDYYISVSGNRQDGYQDNSQQARERINANIGLQLGNHQ
                     EIRAYFLQANVAERIPGSLTTGQLFQNRQQAGGQSPPGTPPFFACNQNNQVCNYGRYY
                     NLQRIGIAYHNEFAPNQYFEIIPYFSNQYVDHPIFQTIRQENNNVGGEFRYVNSNSLF
                     GKNNSFVAGFQPRYGNQRQQRFVNINGSIGALTQNYTAKTTYFGMYAEDAFDATKDFT
                     IVLGGRWDYTGRQATVDNFGPAGNPFNPNTPSTPTGTNRPLQHFDAISPKIGFVYRTT
                     PTSQLYFNASRAYEAPLNLELLSSVNANGTPNTGFLNLDAQRAWQLELGHRGTSADKR
                     YTWDVTVYNLEMQKEILASIINNQGTFQNANGTRHTGVEAGGGMVLGKGLFAQGGAGR
                     EDSLQTRVAYTWSRFKFTDDVRGGGAVGPNVLIAKDGNTVAGAPEHSLNVEFRYDHPS
                     GWWIAPNIEWSLSGFYTDYQNTIKNPSYALINLRSGWNVDEHWTLFAEGRNLTDKTYA
                     GAVVVNDQFNRYANAGLGISAFGGVEYKF"
BASE COUNT          754 a          979 c         1133 g          680 t
ORIGIN      
        1 aagttctttg ccgagatcga caatatcgcc gacaagaaat atgcggcctc cggcaacgtg
       61 gtggccgata gcacggccga tgcgaacaag acgttgttct ttgccgggta tggccggtcg
      121 ttttatgccg gggtgacatt gggattgttc taaccatatg gcgcgagcgg tgcccggatg
      181 cggggccgtt cgcgccctta acaaggagat tgccatgaac aagcaactct tgcgggtcgg
      241 tgaggcggcg gatgtgttgg ccgtgagccg ctggacgatc tatcggtggg tggaggaagg
      301 gcggttggag ggcaccaaga tcggccgcgg gagcctcagg gtgtttcgca cgtcgctgga
      361 ccggttggtg caaaacaata agacgcagga aatgaacctg accgtctgat gcgtgtctcg
      421 caaaggctgc acctggcccg tgagaggtcg atgcgggctt gtgtgcgcga gtgagaggac
      481 ctatgcatcg cattctgttg tgtcttgtgt ttctgctggc cggttgcgcg ggagggacgg
      541 gcctatcctc atcgtcgaag gcctcgcagc agaccgggcc gtccgggagc gattcctatg
      601 ccttcaccaa cggccgatgg ttcgacggga aagggtttca ggcggccact tggtattcag
      661 tccagggacg tttgacccgc accccgccgc aggggaaggt cgaaacggtg gacctctccg
      721 gcctgttcgt cgtcccgccg ttcggtgaag cgcacaatca caacgtggaa ggtccatgga
      781 atgttcgggc ggtggcggag cggtatctca aggacggcgt gttctatgtg aagaatccga
      841 acaacgtccg ggacctcgcc ctggaaactc gcggcgcggt caatcgaccg acgagcatcg
      901 atgcgacctt tgcgcacgca gggctgacgg ggcaaggagg ccatcccatc gcgttgtatg
      961 aggatgtgtt gcgcctgggc cggtacgaac cggccatcgg gacggtcgag cgaggctggt
     1021 ttgagaaccg gtcgtacatc gtgctcgaca ccgacgcgga tctcgagacg aaatggccgt
     1081 tgatcacgag tggccgacct gacttcctga aagtctatct cgtgcattcc gaagatgagg
     1141 acgcgaccgg gaagtccgga cattccccgc agcgaagcgg tttgcatcct cgactcgtgg
     1201 cacagattgt cgccaaggcc catgcggcag ggctacaggt gacggcccat gtcgaaacgg
     1261 cggcggactt tcgccatgcg atccgtgcgg gagtcgatga gattgcgcat gtgccgggat
     1321 ggttggtgaa cagtgccgcc gatgcggagt ccgcccgact cacggaagcg gatgcgcggt
     1381 tggccttgga acgtcacgtg cgggtcgtga ccaccgctgt ggcgggaagc gccatgccct
     1441 ccgtgacagg acatcacccg cacgggcact acgaagggca tgccaccggg ccgtccgatc
     1501 atcacgcaac ggcgccggat gaatcattgt cacaggttct caaggacaac atcgcgttgc
     1561 tccatcgcgt gggagtgcgc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nntacaaggg cgcgaacgcc ctgcggtacg gcgcgaacag catcggtggc gcgatcaatt
     1741 tcgtgccgcg caccgggtat agcgcctcca ccctgcaaat gcggatgctc ggcggcagct
     1801 tcggcatggt gagcggccag gtgtccagtg gcaaggtctt gcagcccttc aaggtgggca
     1861 acatgagcgc gacgatggat tactacatca gcgtgtcagg caatcgccag gacggatatc
     1921 aagacaacag tcagcaggcc cgggagcgca tcaatgccaa catcggcctg cagctcggca
     1981 accaccagga gatccgcgcc tactttctgc aggcgaatgt ggcggagcgc attcctggat
     2041 cgttgaccac gggacagctg tttcaaaacc ggcagcaggc gggcgggcag agccctcccg
     2101 gcaccccgcc gttttttgcg tgtaatcaga ataaccaagt ctgtaactac gggcggtact
     2161 ataacttgca gcgtatcgga atcgcgtacc acaacgagtt cgccccgaac caatattttg
     2221 agatcattcc ctacttctcg aaccagtacg tagaccatcc tatctttcag acgatccgac
     2281 aggagaacaa taacgtcggc ggtgagttcc ggtacgtgaa ctccaactcg ttgttcggta
     2341 agaacaactc gttcgtcgcc ggattccagc cgcgatacgg caatcagcgg cagcagcggt
     2401 tcgtgaacat caacggcagt atcggggcgc tgacgcagaa ctatacggcc aagacgacgt
     2461 atttcgggat gtatgcagaa gatgcgtttg atgccaccaa ggatttcacc atcgtgctcg
     2521 gcggccggtg ggattacaca gggcgtcagg cgacggtgga taatttcggg ccggccggga
     2581 atcccttcaa tcccaatacg ccgtccacgc cgaccggcac gaaccggccg ttacagcatt
     2641 tcgatgcaat cagcccgaag atcgggttcg tgtatcgcac aacgccgacc tcgcaattgt
     2701 atttcaatgc cagccgggcc tatgaggcgc cgctcaatct ggagttgctc tcctcggtta
     2761 atgcgaacgg cacgccgaat acaggcttct tgaacctcga tgctcagcgg gcatggcaat
     2821 tggaactcgg tcaccgagga acatcagcgg acaagcgcta cacctgggat gtgaccgtct
     2881 acaacttgga aatgcagaag gaaattctgg cctcgatcat caacaaccaa ggaacgttcc
     2941 aaaatgcgaa cggcacgcgg cataccggcg tggaagccgg tggcgggatg gtcttgggga
     3001 aaggtctgtt cgcgcaaggc ggagccggac gagaggacag cctgcagacg cgtgtggcct
     3061 acacctggtc gcgtttcaaa ttcaccgacg atgtgcgggg cggcggagcg gtgggtccga
     3121 atgtactgat cgccaaggat gggaatacgg tcgccggcgc gccggaacat agcctgaacg
     3181 tggagttccg gtacgaccat ccgtcaggct ggtggatcgc gccgaatatc gaatggtcgc
     3241 tgtccggctt ttatacggac tatcaaaaca ccatcaagaa cccctcctat gcgcttatca
     3301 atctgcgttc tggatggaac gtcgatgaac actggacgct ctttgccgaa ggacgtaatt
     3361 tgaccgataa gacctatgcc ggcgcggtgg tggtgaacga tcagttcaat cggtatgcca
     3421 acgcgggatt gggcatcagc gcgtttggag gagtcgagta caaattctaa ccggctgtgg
     3481 aagcaggctg caggcagcgt gctcgcatgg cacgcagggg cccatcgtcg gatgggctcc
     3541 tgctgcaccg ttcgcgaaag gtggtgaatc agccacgcat cgctcctggc ggggtgatgc
     3601 tgctcgaaaa atgggataga cgatcgtcgt ccgaacatgg gataaa
//
LOCUS       DAOQRM010000217         3624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_28357_length_3624_cov_21.299243, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000217 DAOQRM010000000
VERSION     DAOQRM010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28357_length_3624_cov_21.299243"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PKK81_12495"
     CDS             complement(<1..470)
                     /locus_tag="PKK81_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33751.1"
                     /translation="MPTRRKRPTAASTPPAIIRPIDASRLKTYPLQRRHSKVQVSNFA
                     KVWTKGRSFARFLDSLPDILAVKTLREVAKAIAKAHRNGKPVIVGMGAHVVKVGLGPL
                     LVDLMERGIVTALAMNGAVIIHDFELAFMGHTSEEVDAEIDSGRFGMAEETGRML"
     gene            complement(454..1032)
                     /locus_tag="PKK81_12500"
     CDS             complement(454..1032)
                     /locus_tag="PKK81_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNV33752.1"
                     /translation="MKEFAIKAFDDSAEIKRRFARDHADRIVQVAQLIGGAFQAGHKV
                     LLFGNGGSSTDAAHIAAEFVGRYKRERAPLPAIALATDIAAITCIANDYGFEELFARQ
                     VQAHGQRGDVAIAISTSGNSPNVLKGVEAAKALGLTTIAWTGGTGGKLAGMVDYAFVV
                     PSTLTARIQESHITLGHVLCELIEDHVLANPA"
     gene            complement(1087..2025)
                     /locus_tag="PKK81_12505"
     CDS             complement(1087..2025)
                     /locus_tag="PKK81_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV33753.1"
                     /translation="MPGGLWVVIFLAGLVLLVISKTARVVPQQSAYVVERLGRYSRTL
                     GAGFHILWPFLDSVQYKHSLKETAIDIPEQICITRDNVQVGVDGILYSKVLDPQRASY
                     GISDYRFAITQLAQTALRSEIGKIELDRTFEERTNINSQVVNELDKATEPWGVKVLRY
                     EIKNITPPKDVLAAMEKQMRAEREKRAVILTSEGERDAAINQAEGEKQQVIKASEAKK
                     QQQINEAEGAASAIMAIAGATADGLRKVAESTQIPGGYEAVQLRVAEQYITKFGELAK
                     ASNTLVLPANVSDVGSMLTLAMNMITKRSSPTSPGK"
     gene            complement(2053..2487)
                     /locus_tag="PKK81_12510"
     CDS             complement(2053..2487)
                     /locus_tag="PKK81_12510"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNV33754.1"
                     /translation="MTWWLWAFLGLFLLGSEIVTPGGFYMLFFGIGALVVGALVGLGM
                     IHSEWMSWLLFSVFSVASLVILRPPLRRLMTADRGNVSSVDTMGGETAIVLDDLPPGA
                     TGKAECRGSTWNAHNAGDKPLLKGQRSLVDRVDGITLWIKPE"
     gene            2630..>3624
                     /locus_tag="PKK81_12515"
     CDS             2630..>3624
                     /locus_tag="PKK81_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HNV33755.1"
                     /translation="MPADTPVKALILAFTDAPSLAAYVINRLQPELLCFFVPESAKTL
                     VEEAVQPLVQQMPKRWDWVVTPDPADVIACHQALSRSIHDLFRNWAVQVGEVVVDLTG
                     ATPAMAAALATVSQPWTSRVISLVDAEGREEGEAIAIGGLAKYWLQGNPWDESAVVVR
                     REASDAFNHGSFRAAATLFHTLEARVSGGQKPLYRALGELALGYGLWEQFHYRQAWDK
                     LKTSLKALDMASLWGGPPGLKALLPFIKANNGFLEKLVLDPADVKEGVVLDLLAHAHR
                     RAHVDHDYERAMVALVRAMEACAQRQLFKQYKIKTWDVQPEQLPDAMRDTCRTCYL"
BASE COUNT          641 a         1064 c         1184 g          735 t
ORIGIN      
        1 agcatgcggc cggtttcttc cgccatgccg aatcggccgg agtcgatctc cgcgtccact
       61 tcttcggacg tgtgccccat aaacgcgagt tcgaagtcat ggatgatcac ggctccgttc
      121 atggcgagag cggtgacgat gccgcgttcc atcaggtcca cgaggagcgg gccgagcccg
      181 accttcacga cgtgggcgcc catgccgacg atcaccggct tgccgttgcg atgggctttg
      241 gcaatggcct tcgcgacttc gcgcaacgtc ttgactgcca ggatatcggg gagcgaatcg
      301 agaaaccggg cgaacgagcg acccttggtc cagaccttgg caaagttgga gacctgcacc
      361 ttgctgtgtc ggcgctggag cggataggtc ttcaggcgcg acgcatcgat cggccggatg
      421 atcgcggggg gcgtcgatgc tgcagtgggg cgcttacgcc gggttggcaa gaacgtgatc
      481 ctcaatgagt tcgcagagca catggcccag cgtgatgtga ctttcctgaa tgcgggcggt
      541 cagcgtggaa ggcacgacga aggcgtagtc caccatgccg gccagtttgc cgccggttcc
      601 gccggtccag gcgatcgtgg tgaggcccag ggctttggcc gcctcgaccc ctttcaacac
      661 gttgggggag ttgccgctgg tgctgatcgc aatcgccaca tctccacgct gcccgtgtgc
      721 ctgaacctga cgggcgaaca gctcctcgaa gccgtagtcg ttggcgatgc aggtgatggc
      781 ggcaatatcc gtggcgaggg caatggccgg caacggggca cgttcgcgct tatagcggcc
      841 gacaaactcg gcggcgatgt gcgcggcatc ggtggagctg ccgccgttgc cgaagagcaa
      901 gactttatgt ccggcctgaa acgcgccgcc gatgagctgc gcgacctgca cgattcggtc
      961 ggcatggtcc cgcgcgaagc gccgcttgat ctcggcgctg tcgtcgaagg ccttgatggc
     1021 aaattctttc atcgtccgga attgtaggga agccccgagt cactgtcaaa cgaccgcaga
     1081 cgtcttttac tttcccggag atgtgggcga agaccgtttc gtgatcatgt tcatcgccag
     1141 cgtcagcatc gacccgacat ccgagacgtt ggcggggagc acgagggtgt tgctggcttt
     1201 ggccagttcc ccgaacttgg tgatgtactg ctcggcgacg cgcagctgca cggcttcgta
     1261 gccgcccgga atttgcgtgg attcggcgac tttgcggagt ccgtctgccg tggcgccggc
     1321 gatggccata atcgcggagg ctgcgccctc ggcctcgttg atctgctgct gtttcttcgc
     1381 ctcggaggcc ttgatgacct gctgcttctc gccttccgcc tggttgatgg cggcgtcgcg
     1441 ttcgccctcg gaggtcagaa tcaccgcgcg tttttcccgt tcggcccgca tttgtttttc
     1501 catcgcggcc agcacgtctt tcggcggcgt gatgttcttg atctcgtacc gcagcacctt
     1561 cacgccccag ggttccgtcg ccttgtccag ctcattcaca acctggctgt tgatattggt
     1621 gcgctcttcg aaggtgcggt cgagttcgat tttgccgatc tcgctccgga gggcggtctg
     1681 agccagctgc gtgatcgcaa agcgatagtc gctgatgccg taggaggctc gctgcggatc
     1741 cagcaccttg gaatagagaa tgccgtcgac gccgacctgt acgttgtcgc gcgtgatgca
     1801 gatctgttcg ggaatatcga tggcggtttc tttcagcgag tgtttgtact gcacactgtc
     1861 gaggaacggc cacaggatgt ggaaccccgc gccgagtgtc cgtgagtacc ggcccagccg
     1921 ctcgacgacg taggcgctct gttgcggcac cacacgcgcg gttttcgaga tgacgagcag
     1981 gacgaggcct gcaagaaaaa tgacaaccca gagtccgcct ggcattccgc tatcctccat
     2041 gatggtgcga cctcattcag gtttgatcca cagggtgatg ccgtctacgc gatccacgag
     2101 gctgcgttgt cctttgagca gcggcttgtc gccggcgttg tgcgcattcc aggtgctgcc
     2161 ccggcattcc gccttgcccg tggcgccggg aggaaggtcg tccaggacga tggcggtttc
     2221 gccgcccatg gtatcgaccg atgagacgtt gccccggtcg gccgtcatga gacggcggag
     2281 cgggggacga aggatgacga gggaagcgac cgagaagacc gaaaacagga gccaggacat
     2341 ccattcactg tgaatcatgc ccaggccgac cagcgcgccg acaaccagcg ccccgatacc
     2401 gaagaacaac atgtaaaacc cgccgggtgt gacgatttcg ctgccgagca gaaacagtcc
     2461 cagaaaggcc cagagccacc aggtcatcgc acctcctcag ccgtcacggg cagcggggtt
     2521 gtctcgtgat tcacgcggaa gtctatgcgt ctttcccggg accgtcaaga actgtgagga
     2581 aagcgtcgtt cgttgaagca gccggagtgc agtggtatag tccgcggtca tgcctgcgga
     2641 tactcctgtc aaagcgctca tcctcgcctt caccgacgcg ccgtcgctcg ccgcgtatgt
     2701 gatcaaccgg ctgcagccgg aattgctctg ctttttcgtg ccggaatcgg cgaagacgct
     2761 ggttgaggag gcggtgcaac cgctcgtaca gcagatgccg aagcgctggg actgggtggt
     2821 gacgcccgat ccggccgatg tcattgcctg tcatcaggcc ctgtcacgat ctattcacga
     2881 cctctttcga aattgggccg tgcaggtggg cgaggtggtg gtcgatttga ccggagcgac
     2941 tcccgccatg gccgcggcgc tggcgacggt gagccaaccc tggacgtcgc gagtcatcag
     3001 tctggtcgat gcggagggcc gcgaagaggg tgaggcgatc gcgatcggcg ggttggccaa
     3061 gtattggctg caaggcaatc cctgggatga gtcggcggtc gtggtgcgcc gtgaggccag
     3121 cgacgccttt aatcatggtt cattcagggc cgcagccacc ctgtttcata cgctggaagc
     3181 tcgcgtgagc gggggacaga aacccctgta tcgtgccttg ggcgaattgg cgttggggta
     3241 cgggttgtgg gagcagtttc actaccgcca ggcatgggac aagctgaaga cctcgttgaa
     3301 ggcgctcgat atggcgtcgc tctggggcgg gccgccgggg ttaaaggcgc tcctgccgtt
     3361 catcaaagcc aataacggat ttttggaaaa gctggtactg gatcccgccg acgtgaagga
     3421 aggggtagtg ctcgatctgc tcgcgcatgc ccatcgtcgc gcccacgtgg accatgatta
     3481 tgagcgggcc atggtggcgc tcgtgcgcgc gatggaagcc tgcgcccagc ggcaactgtt
     3541 caaacagtac aagatcaaga cctgggatgt gcagccggag caattgcctg acgcgatgcg
     3601 cgacacctgc cgcacctgtt acct
//
LOCUS       DAOQRM010000218         3596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_28731_length_3596_cov_14.127648, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000218 DAOQRM010000000
VERSION     DAOQRM010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28731_length_3596_cov_14.127648"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1893
                     /locus_tag="PKK81_12520"
     CDS             128..>1893
                     /locus_tag="PKK81_12520"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNV33756.1"
                     /translation="MAVESRTFTDAERMELRSHVELAGEAISSYWPMRTFIHHNPLHG
                     LEDLPFAQAVKRGEQLLGGKGYLPSASYRRYFEQGRIRQEDLTRVLAPLASERRVLFA
                     GREISHLDLLRLSMVHGLSEAAEAYADPSVPVEDRLDQLALWLATAVGQTRTSQADTL
                     IPWETVDLLSHETLSTWCDRTLGTSLVSDINEQMIKWCSAFLDEGEASWTMPDRQHTF
                     YRAWKRLARYDASLRLLGVADAAQKIDALGDRPEEAVLDSLAVMGIPKSAWESYFALH
                     LSAMPGWTGYIKWRSEEQHHPWQARYPVDLVKYLAVRLFYEREFVALHCRQSLDIAGH
                     YDAIRASIEQAPYAHWLRRQLTEGRLPASAAAEAREWRRLHRTAQPDEWNGIGRQVYE
                     QTAGWRASEAVKRDVRRLWALSAAMGVDPEQISETQPTDVSTILNWLDGFPAGQQSQR
                     WLEAFEARHRRDIVGQLRSVATQLRDAGDRVSSGGSSRPLAQMVFCIDIRSEVLRRHL
                     EQLGGYETLGVAGFFGIPVKYQAFGEEHPVTHSPVLLKPKNHIREIPRSYHGAAASRH
                     RLFARLNKAGHELLHDLKENVIT"
     assembly_gap    1894..1993
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1994..3343
                     /locus_tag="PKK81_12525"
     CDS             <1994..3343
                     /locus_tag="PKK81_12525"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNV33757.1"
                     /translation="GSAITPALIETIRLGALENQDEQSRGEVANALGIAPSEADALHE
                     RLRRELALTPRGMSTRMQRITLHGFSVAEQAYGVEAALRLMGFTSGFSRLVVMCSHGS
                     TSDNNPYESALDCGACGGNSGLPNARAFASMANNPAVRKVLESRGVKIPGDTHFVAAL
                     HDTTRNDVRIVDLEDVPPTHRKDLVRLLEDLKEAGTQAALERGLALERPGAKPTRRDP
                     QQRASRRSVDWAQVRPEWGLSKNNLLIIGRRELTRPLDLQGRSFLHSYDYRQDSSGKL
                     LETIMTAPLIVAQWINMEHYFSTVDNEVYGSGSKVYHNIVGRVGVMSGATSDLRLGLP
                     AQTVLDGPVPYHEPMRLLTVIEAPRALVESVIAKHPGLERLFQNEWVSLVVCEPNEAA
                     FYHYDIMQGWRVVSEESELRQELEGSSGRRPDADESGAQPGSGGPVREGEEPAASRP"
BASE COUNT          648 a          977 c         1193 g          678 t
ORIGIN      
        1 tgcgcgtccg ggcttacgtg atgtttctca acggcctcta tgttgaggat ctggttcgca
       61 tgatcggtcg ggtcgccttt cgccggtagt ccggctgagc gcggtcaggc tgaaacggag
      121 tggtggcatg gcggttgaat cacgcacctt cacagatgcc gagcggatgg agttgcgctc
      181 ccatgtggag ttggccgggg aggcgatttc gtcctactgg cccatgcgca cgtttattca
      241 ccacaaccct ctgcacgggc tggaagatct tccgttcgct caggcggtga aacgcgggga
      301 acagctgctc gggggaaaag gctatctgcc cagcgcctcg tatcgtcgct atttcgaaca
      361 gggccgcatt cgtcaggagg acctcacccg ggtgcttgcc cccttggcca gcgagcggcg
      421 ggttctgttc gcggggcgtg aaatttccca tctggacctg ttgcggctct ccatggtgca
      481 cgggctcagt gaggcggctg aggcgtatgc ggatccttcc gtgccggttg aggacaggct
      541 ggatcagctg gccttgtggc ttgcgactgc ggtggggcag acgagaacca gccaggcgga
      601 tacgctgatt ccctgggaga cggtcgattt gttgtcccac gaaactctct caacctggtg
      661 cgatcgcacg ttggggacat cgctcgtgtc ggatatcaac gagcagatga tcaaatggtg
      721 cagcgcattt ctggatgaag gggaagcctc ctggacgatg ccggatcggc aacatacctt
      781 ttatcgcgcg tggaagcggt tggcccgtta tgacgccagc ctgcggttat tgggtgtggc
      841 ggacgcggcg cagaagatcg atgccttggg tgatcggccg gaagaggccg tgctggatag
      901 tctggccgtg atgggcatcc cgaagtcggc atgggagtcc tatttcgcct tgcatctgtc
      961 cgccatgccc ggatggaccg gctatatcaa gtggcggtcg gaggagcagc atcatccctg
     1021 gcaggcccgc tatccggtcg atctggtgaa atacctggcg gttcggttgt tttacgaacg
     1081 cgaattcgtg gcgctgcact gtcgacagtc gctggacatc gccgggcatt acgatgcgat
     1141 ccgtgcgtcc atcgaacagg ctccctatgc ccactggctc cggcgtcaat tgacggaggg
     1201 acggttgccc gcctcagccg ccgcagaggc gcgggagtgg cgtcgtcttc atcggactgc
     1261 ccagccggat gagtggaatg gcatcgggag acaggtctat gaacagaccg ccgggtggcg
     1321 ggcttctgaa gccgtgaagc gagatgtccg gcggttgtgg gccctgtcgg ctgcgatggg
     1381 tgtcgatccg gaacagatca gcgaaacgca gcctaccgat gtgtcgacga tactgaactg
     1441 gttggatgga ttccccgccg gacagcagag ccagcgttgg ctggaagcgt ttgaagcacg
     1501 tcaccgccgg gacatcgtcg ggcaattgcg cagcgtcgcg acgcagctgc gggacgccgg
     1561 cgatcgggtc tcgtcgggtg gatcgtcacg tcccctggcg cagatggtgt tttgtatcga
     1621 tattcggtcc gaggtgttgc ggcggcatct cgaacagctc ggaggatacg agacgttggg
     1681 ggtggcggga tttttcggca ttcccgtcaa gtatcaggcg ttcggcgaag agcacccggt
     1741 cacgcattcg cccgtgctgt tgaaacccaa aaaccacatc agggagatcc cgcggagtta
     1801 ccatggggcg gcggcgagcc ggcaccggct gtttgcgcga ctcaacaagg ccggtcatga
     1861 gctcctccac gatctcaagg aaaacgtcat tacnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnggttccg cgatcacgcc ggccttgatc gagacgatcc gcctcggggc
     2041 gctggagaat caggacgagc aaagcagggg agaggttgcg aacgcgctcg gaattgctcc
     2101 ctccgaagcg gatgcgctgc atgaacggtt gcgccgggag ttggccctca caccgcgggg
     2161 catgtcgacc cgcatgcagc gcattaccct gcatggattt tctgtcgcgg aacaggccta
     2221 cggcgtcgaa gcggcgctcc gtctcatggg attcacgtcc ggattttcac ggctggtggt
     2281 gatgtgttcc cacgggagta cgtcggacaa caatccgtac gaatccgccc tggattgcgg
     2341 cgcctgcggc ggcaacagcg gcctcccgaa cgcgcgggcc ttcgcgtcga tggccaacaa
     2401 tccggccgtt cgaaaggtgc tggagtcgcg gggggtcaag atccccggcg atacgcattt
     2461 tgtcgcggcg ctgcacgata cgaccaggaa cgatgtccgc atcgtagatt tggaggatgt
     2521 gccgccgacg caccggaagg acctcgtgcg gttgttggag gatctgaagg aggcgggaac
     2581 gcaggcggcg ttggaacgtg gtctggcgtt ggagagacct ggggcgaaac cgacccgccg
     2641 cgacccgcag caacgggcga gccggcgcag tgttgattgg gcccaggtcc gccctgagtg
     2701 gggcctgtcg aagaacaacc tcctcatcat cgggcgtcgt gaactcacgc ggccgctgga
     2761 tctccagggg cggtcatttc tgcattccta cgactatcgc caggattcct cgggcaaact
     2821 cctggagacg atcatgaccg ctcccctcat cgtggcccaa tggatcaaca tggagcatta
     2881 tttctccacc gtcgacaacg aggtgtacgg cagcgggagc aaggtgtacc acaatatcgt
     2941 cggacgagtg ggtgtgatga gcggagcgac cagcgacctt cgcctcggcc tgccggctca
     3001 gaccgtcctg gacggtccgg tcccctacca tgagccgatg cgcttgttga cggtgattga
     3061 ggcgccgcgc gccctcgtcg agtcggtgat tgcgaaacat cccggactcg aacggctgtt
     3121 ccagaacgag tgggtgtcgc tggtggtgtg tgagccgaac gaagcggcgt tttaccatta
     3181 cgacattatg cagggctggc gagtggtgtc ggaagaatcg gaactgcgcc aggaattgga
     3241 ggggagttcc ggtcggcgtc ccgatgcaga tgagtcggga gcgcagccgg gatccggcgg
     3301 gccggtgcgg gagggcgagg agccggctgc ctcgcgcccc tgacgcaaga cagggcggaa
     3361 tgagcgtaaa tagtctttgg catcgagggg cctgaaagac aggaggaggg gggcgaaagt
     3421 atgctgacgt tgcatccgat gaaagaaatc cgcatcgtgg tcgagggtga gcacctcaag
     3481 ttagtgacga acctgctcga tcgagtcggc gccaccggct atacgatcat caacaacgtc
     3541 tccggcaagg gacatcacgg gttccacgaa ggccacctgc tgttcgacga caccag
//
LOCUS       DAOQRM010000219         3590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_28811_length_3590_cov_15.848091, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000219 DAOQRM010000000
VERSION     DAOQRM010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28811_length_3590_cov_15.848091"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..779)
                     /locus_tag="PKK81_12530"
     CDS             complement(183..779)
                     /locus_tag="PKK81_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV33758.1"
                     /translation="MKGTGFFTGLVVGIVATVLLSQWTMSAASKEAGTAPCLPAEMVA
                     DYVHSVIQADREFYTTDIVERMQLRGIVFAAENWRETSRLPLPAQFLLESGRLVAQQR
                     NGIRFRLISDWAINKTNRPATDFERAGLAEILINPDRPHTGVTTEGGRSVFQALYPDK
                     ALSQTCVSCHNAHPNSPKKDFKPHDVMGGILLTLPLPH"
     gene            1022..1456
                     /locus_tag="PKK81_12535"
     CDS             1022..1456
                     /locus_tag="PKK81_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HNV33759.1"
                     /translation="MTGKFDPESHEWARHEHVVVFDGVCNWCNAWVNFLIDHDPHETF
                     KFGTLQSDRGQQILHTLQLSTEDFSTFLLLEQARVYTKSTAALRIVRHLPGCWPLFYL
                     GILIPRPLRDALYDYVARHRYRWMGKTDACRVPSPNQRRRFV"
     gene            1601..2059
                     /locus_tag="PKK81_12540"
     CDS             1601..2059
                     /locus_tag="PKK81_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV33760.1"
                     /translation="MNHLFTASIFVAIAMALSVPSPAVAQNFPPSVTQKIQAAQKQVK
                     TIGLEEYRKVVESPGEALIIDVREPQEFAAGHVPGAVNIPRGLVEFRIWKQVGFPANP
                     DTNRPVYLQCQNGNRASLAAQSLAELGFTNTTAVVMSFDEWQQAGHPLVK"
     gene            complement(2258..>3590)
                     /locus_tag="PKK81_12545"
     CDS             complement(2258..>3590)
                     /locus_tag="PKK81_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33761.1"
                     /translation="GGALSVRLASIIGLLTAGLLLCAPWSLAGTIRVDLGVEQGPMNH
                     RANGYLVSIEPTDPPMELILPLKPTSFRGNTGYVLSNYDRLKQAGVTEFQLTLGLVFE
                     HRALQIFDINQIGLDGNYEPWLAHVDHVIDQVLQRQLSVIWDIYNEPDLAAVPLNDSE
                     RLKEGWRLAYQRIKQRIPGAQIVGPSVSRYRLLKPFLEWSDSQGVFPDVVAYHEYADP
                     SDAIRYVNDLRDYLKAHGLARPISVNEILGQESWTSAGYTAGVIAAYERADILSAMRS
                     CWPDPQDMSRSYVENTCDNPTLDGLLYVDRKQKRPGWHVYKTYAEMEGVRLYTMTDSP
                     KLHALAALDKAAGTLRLLLGKYEDDSPGDTQVVLKNIGRLFSSQHSGTVHLCAEQIPD
                     VGSGPLEAPVVTLDRALSVENEELSFTLPQFYHSDAYRVVLSLSEPCRASH"
BASE COUNT          767 a         1101 c          987 g          735 t
ORIGIN      
        1 aaattgctcg atgagttgcg tgaggtacgc attcgtgtgc gtaaattgcg cgtggacgat
       61 gtccggcata gagtctctcc ttaagatatc ctcagcaggc tgccggctcg ttccgacgca
      121 ctgccggccc actcctcccc cgttcgcacg accggagagg cggcctgtcc tcgctgcgcg
      181 ggctagtggg gaagcggaag ggtcagcagg attcccccca tcacgtcatg cggcttgaaa
      241 tctttctttg gactgttcgg gtgcgcgttg tggcaactca cacaggtctg ggacagggcc
      301 ttgtccgggt acagagcctg gaagacggat ctgccccctt cggtcgtcac accggtatga
      361 gggcgatcgg gattgatcag gatctcggcc agaccggccc gctcgaagtc ggtggccgga
      421 cgattcgtct tattaatcgc ccaatcgctg atgagccgga atcgaattcc attgcgttgc
      481 tgcgcgacga gccgacccga ttcgagcaaa aactgagcag gaaggggaag cctggaggtc
      541 tctctccagt tttctgcggc aaagacgatt ccccgcaact gcatccgctc cacgatgtcg
      601 gtggtgtaga actcccgatc ggcctggatc acgctatgca cataatccgc gaccatctcg
      661 gccggcaaac agggggccgt ccccgcctcc ttgctcgcgg cactcatcgt ccactgactc
      721 aacagaacgg tggccacgat gccgactacg aggccggtga aaaatcctgt acccttcatg
      781 ctgcacccac ctttcttgtc cgcgcgtggt tgcctcgctc cccaaccgca gccataaacc
      841 cgcccggcca gaacttgcgg gtcgcctccc gcaaaagcat acgccgacac agcagagaaa
      901 tcgagattat ggaccggcct gtttccgaac cggtacaatg ccggtggtga tatcgtctga
      961 acaatgtgat tccaatccgt ggagagaaga atcagcagtc gcttcagcgg gaatgcagcc
     1021 aatgacaggc aaatttgatc cggaatccca tgagtgggcc aggcacgagc acgtcgttgt
     1081 attcgatggt gtgtgcaatt ggtgcaatgc ctgggtgaac tttttgatcg atcacgatcc
     1141 tcacgagacg ttcaagttcg gcaccctaca atccgaccgg ggacaacaga ttttgcatac
     1201 actgcagctt tcgaccgaag acttttcgac cttcctcctg ctggagcagg cccgcgtcta
     1261 caccaaatcc acggccgcgc tgagaattgt gcggcacctc cccggctgct ggcccctatt
     1321 ctatctcggc atcctgatcc cacgccccct gcgtgatgcg ctgtacgatt atgttgcgcg
     1381 tcatcggtac cggtggatgg gcaaaaccga tgcatgccgg gtcccctccc cgaaccagcg
     1441 cagacggttc gtgtagtgcc tgcgggcaga gagtttcgcc gcacgcaacg cacgttcgtg
     1501 ccttgagcca ttcacgggaa cggcgtagac tccgctttgt aacgatcgcg ccggcatcga
     1561 ggccgtgccc attctcatac ctgatgaaga ggaagaacct atgaaccact tgttcacggc
     1621 atccatattc gtcgccatcg ctatggcgct gtctgttcca tcaccggcgg tcgcacaaaa
     1681 ctttcctccg tctgtcacac aaaaaattca agcggcgcag aagcaggtca aaacgatcgg
     1741 cctggaggag tatcgaaaag tcgtggagag tccgggcgag gcgttaatta tagatgtgcg
     1801 cgaaccgcag gagttcgcgg ccggacatgt gccgggcgcg gtcaacattc cccgcggact
     1861 cgtcgaattt cgaatctgga agcaggtcgg gtttccagcc aacccggaca cgaacagacc
     1921 tgtctacctg caatgccaaa acggcaaccg cgcgtccctc gcggcccagt cacttgcgga
     1981 gttggggttc accaatacca cggccgtcgt gatgagcttc gatgagtggc aacaggccgg
     2041 ccatcccctg gtgaagtaag ccatcccctc gcgcgcgatg agcgcttcgt gacaccactc
     2101 acggctccgc cgacacatgc cctcttggtg tgtttattca ggcaggaatt attcccctct
     2161 gacatccggc aagtaatatc aaatgcgttg catccgttgt gaacgggcac tgcatcaatc
     2221 catcgccaac agccggtaag taggtcggcc gttccgctca atgcgaagcg cggcacggtt
     2281 cggagaggct caggacgacc cgataggcat cggaatgata gaactgaggc agtgtgaacg
     2341 agagctcctc attctccact gagagagcac gatcgagggt gacaacaggc gcttcgaggg
     2401 gaccggatcc tacatctggt atttgctcag cgcagagatg caccgtcccg ctgtgctgcg
     2461 aggaaaacag acgcccgata ttcttcagca cgacttgtgt gtcgccaggc gaatcgtcct
     2521 cgtattttcc caacaagagt cgtaacgtgc cggcggcctt atccagcgcg gcgagggcat
     2581 ggagcttggg actatccgtc atcgtgtaca gccggacccc ctccatctcc gcataggtct
     2641 tatagacgtg ccaccccggc cgtttctgct tccgatcgac atacaacaat ccgtccagcg
     2701 tagggttatc acaggtattt tccacgtagc tccggctcat gtcctgagga tcaggccaac
     2761 aggagcgcat cgcgctgagg atgtccgccc gctcataggc ggcaatcacc cccgctgtat
     2821 aaccggcact cgtccaggac tcctgcccca ggatctcgtt cactgaaatc ggccgcgcca
     2881 gaccgtgagc cttcaaatag tcgcgcagat cattcacgta cctgatggca tccgatggat
     2941 ccgcatattc atgataggcc acgacgtcgg gaaagacccc ctgcgaatca ctccactcca
     3001 gaaagggctt gagtaaccgg tatcgactca cgctcggccc gacgatctgc gcgccgggaa
     3061 tccgctgctt gatgcgctga taggccagcc gccagccttc tttgagtcgt tcgctgtcat
     3121 tgagaggaac cgctgcaaga tcgggttcat tgtaaatatc ccagatcacc gacagttgtc
     3181 gctgcagcac ttgatcgatc acatgatcca catgcgccaa ccagggctca tagttcccgt
     3241 cgagaccgat ctgattgata tcaaaaatct gcagggcgcg atgctcgaag accagtccga
     3301 gggtcagctg aaactccgtc accccggcct gtttgagccg gtcatagtta ctcaacacat
     3361 atccggtgtt tccgcgaaaa ctggtgggct tcaacggaag aatcagctcc atcggcgggt
     3421 ctgtcggttc gatgctgacc agatacccgt tagctcgatg gttcatcggg ccctgctcga
     3481 cccccagatc gaccctgata gtgccggcca gactccaggg cgcgcacaac aacagtccag
     3541 ctgtcagcag gccaatgatc gaggcaagtc gcacagacag ggctcctccc
//
LOCUS       DAOQRM010000220         3575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_28985_length_3575_cov_13.508239, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000220 DAOQRM010000000
VERSION     DAOQRM010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28985_length_3575_cov_13.508239"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..>1753)
                     /locus_tag="PKK81_12550"
     CDS             complement(391..>1753)
                     /locus_tag="PKK81_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HNV33762.1"
                     /translation="QYGNGVVDVTTRQQVQLRHLTIAHVPSVFSKLEEAGLTSLQTGM
                     DTVRNIMTCPVAGLNPNELLDGTDIVRAINHEVLGNPAYTNLPRKCNIAVTGCPDNCL
                     HTETQDIALVPAYHDLGHDKCYGYNVLVGGKLGSGGYRIAGSLDMFVNPAEAFEVCRT
                     LLHIYRDHGPRENRTQARLAFLVEAWGEARLRHEVERLVGRTLPTAGVDARGAAERDH
                     IGIFRQKQRGMNYVGLKVLVGRVKAADLRSIAALAVRYGNGEVRLSPAQAFVIPHISD
                     RLVGELAEEPLVKQFAYNPSALYKGLVSCVGSDYCNLAVIETKSRAVETARVLERKLG
                     DTLKPITLHWSGCPAGCGNHLVADIGLLGKKAKVGGKVVDAVDVFVGGRSGPDPKLAT
                     KIMEDVPCDRLPAVLEMIMPYHTREKMHRVRGKAVPKGTGASKSTASGNETTGLTPQP
                     LPA"
     assembly_gap    1754..1853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1854..2043)
                     /locus_tag="PKK81_12555"
     CDS             complement(<1854..2043)
                     /locus_tag="PKK81_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33763.1"
                     /translation="MSTNAETAVLTVLEHQAPQTFEQLMALSGLSASQVLLVVDQLSR
                     AGAVVLRKSGPDYSVALGG"
     gene            complement(2095..2535)
                     /gene="cynS"
                     /locus_tag="PKK81_12560"
     CDS             complement(2095..2535)
                     /gene="cynS"
                     /locus_tag="PKK81_12560"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: protein motif:HMM:TIGR00673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HNV33764.1"
                     /translation="MEKAAVRKAIKAQRLNKKVTIAEVAKTVGKNPTFVAAALNGNHR
                     LSPEEAGKVGKLLDLDKEQISSLSAFPVRADFPNATDPFKYRLLEVIGVYGDSMREMA
                     NEMFGDGIMSAIDFTLDMEKVTGSQGEARCKITLNGKWLEYKTF"
     gene            complement(2589..3419)
                     /locus_tag="PKK81_12565"
     CDS             complement(2589..3419)
                     /locus_tag="PKK81_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNV33765.1"
                     /translation="MHTADHERIAGVAARKWSFFERSPGGYLILSALAGIYLGFGIAL
                     IFSVGGPLAAAGSPVVKLVMGVSFGIALTLVIFAGSELFTGNNLIGTIGGLTRSLTWA
                     QVMQLNAWSWLGNLAGSLGLAWLIVQSGVFAKGPTTELIEKVASVKMSLPAWELFVRG
                     ILCNWLICLAVWMTGRTSNDTAKILLIFWCLFAFIGTGFEHSIANQSLLGMALFLPHD
                     AAVSWGAFWYNQLFVVLGNLVGGGLFVGGLYWMVSPYRVTEATAAPVAQPVVSAVAGS
                     "
BASE COUNT          696 a         1155 c          900 g          724 t
ORIGIN      
        1 gtctgctcat cgaatccgtc cagcgaggcg gtggccttac gtcgggataa gaatggctgg
       61 atcgccacca acgtcgcttg aatctgctct atgtgccggc tgagaagatc cgccggttcc
      121 tgactcgatt tcactctctt ggccatgctg cctccttgtt gagggcaaga gaataacgca
      181 actatggcat cggctcaagc cttcccggca agatgctgaa aatgtccgcc cgctttgttc
      241 tcgcgtcgct caaagcctca acgtacctca aagggtacgc ctcgccttct cgcatcgctg
      301 cggccgcgcg ggacagacat tttgagcatc ctgctgggag cactccctac tgccatctcc
      361 gtgcttacgt tctgcagctt ccaaaactgt ctaggcagga agcggttggg gtgtgagccc
      421 cgtcgtctcg ttgccggatg ccgtcgactt tgaggctccc gtgccttttg gcacggcctt
      481 cccgcgcaca cgatgcattt tttcgcgcgt gtggtagggc atgatcattt ccaacaccgc
      541 tggaagccga tcgcagggca catcctccat gattttggtg gcgagcttgg gatcggggcc
      601 cgagcgcccg cccacgaaca catccacggc atcgacgacc ttgccgccca ccttcgcctt
      661 cttgcccaag agtccgatat ccgccacgag atggtttcca cacccggccg gacaaccgga
      721 ccaatgcaag gtgatcggct tgagggtatc gcccagcttc cgctccagca cccgcgccgt
      781 ttcgacggcc cggcttttgg tttcaatgac ggccagattg cagtagtcgc ttcccacaca
      841 actgaccagt cccttgtaga gggcggacgg gttgtaggcg aattgcttga ccagcggctc
      901 ttccgccagt tcacccacca gccggtcgct gatatgaggg atcacgaagg cttgcgccgg
      961 cgagagacgt acttctccat tgccgtaccg aaccgccaac gcagcgatgc tccggagatc
     1021 agccgccttc acccgaccga ccagcacctt cagcccgaca tagttcatgc cccgttgttt
     1081 ttgacggaag atgccgatgt ggtcgcgctc cgccgctccc ctcgcatcca cccccgccgt
     1141 gggcagggtt ctcccgacca gccgttcgac ttcgtgccgc aaccgagctt cgccccaggc
     1201 ctccacgaga aatgcgaggc gcgcttgggt gcgattctca cgcggtccat gatcgcgata
     1261 gatgtgcagc agcgtccggc aaacctcgaa ggcttccgcc ggattcacga acatgtccag
     1321 ggagccggca atgcgatagc cgccggaccc caacttgccc ccgaccagca cgttgtaccc
     1381 gtaacacttg tcatgcccca ggtcgtgata ggccgggacc aacgcgatgt cttgcgtctc
     1441 cgtgtggagg caattgtccg gacaaccggt gaccgcaata ttgcacttgc gcggcagatt
     1501 cgtataggcc ggattaccca gcacttcgtg attgatcgcg cgcacgatgt ccgtcccgtc
     1561 gagcaactcg ttgggattca ggccggccac gggacaggtc atgatgttcc gtaccgtgtc
     1621 cattccggtt tgcagtgatg tcaggcccgc ctcctccaac ttgctaaaca ccgacggcac
     1681 atgggcaatc gtcagatgcc ggagttggac ttgttgcctg gtggtaacat cgaccacccc
     1741 gttgccgtat tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgcctcc
     1861 caatgcgacg gaatagtccg gtccggattt cctcaatacc accgctcctg cccgactcaa
     1921 ctgatccacg accagcaaca cctgtgacgc gctcaaaccg gagagggcca tcagttgctc
     1981 gaatgtctga ggagcctgat gctccaggac tgtcagtacg gcagtttctg cattcgtgct
     2041 catgatgtcc tcctttcggt tgatcgcgat ccacgcatca gggaccgatt cctcttaaaa
     2101 cgttttgtac tcaagccact tgccgttcag cgtgatcttg caccgggcct cgccctgact
     2161 gccggtgacc ttttccatat ccagcgtaaa gtcgatggcg ctcatgatcc cgtcgccgaa
     2221 catttcattc gccatctccc gcatcgaatc gccgtagaca ccgatgactt ccaacaggcg
     2281 atacttaaac ggatccgtcg cattcgggaa atcggcccgg acgggaaacg cgctcaggga
     2341 gctgatttgt tctttatcca aatccagcag cttccccact ttgcccgctt cctccggtga
     2401 caggcggtga ttcccgttta acgctgcggc gacgaacgtc ggattcttcc cgaccgtctt
     2461 cgccacctct gcaatcgtga ccttcttgtt caaccgttgt gccttgatcg ccttgcgtac
     2521 tgctgctttt tccattgtga tctcccttta gtgattcgcc cgtgaataca ctcccgcacc
     2581 gcgcttcatt acgatcctgc caccgccgac accaccggtt gagctaccgg ggccgccgtc
     2641 gcttccgtta cgcgataggg gctcaccatc caatagagcc ccccgacaaa caacccgccg
     2701 cccaccaggt tccccagcac gacgaacagc tggttgtacc aaaaggcccc ccaactcaca
     2761 gccgcgtcat gcggcagaaa caacgccatg cccaacaacg attgattggc gatgctatgc
     2821 tcgaacccgg tgccgatgaa ggcgaacagg caccagaaga tcaggagaat cttcgccgta
     2881 tcgttgctcg ttcgccccgt catccagacc gccagacaga tcagccaatt gcagagaatg
     2941 ccccgcacga acagctccca ggcaggcagc gacatcttta ccgaggccac tttctcgatc
     3001 agttccgtcg tcggcccttt ggcgaacacg cccgattgga caatgagcca ggccaacccc
     3061 aacgagcccg ccagattgcc gagccaggac caggcattga gttgcatgac ttgcgcccag
     3121 gtgagacttc tcgtcaggcc accgatggtg ccgatgaggt tattgccggt gaacaactca
     3181 gacccggcaa agatcacgag cgtcaatgca atcccgaacg acacccccat cacgagtttg
     3241 accaccggag acccggccgc ggccaacggt ccccccacac tgaagatcaa cgcaattcca
     3301 aacccgagat agatcccggc caaggccgag aggatgagat atccgcccgg tgaccgttcg
     3361 aaaaacgacc acttccttgc tgccacccct gcgatccgct cgtgatccgc tgtatgcatg
     3421 aggctctcct ttcggtggtc actgtgtccg cacaactcaa aaaaaaacgt cctcatcccg
     3481 cattcgggaa gaggacgtcg ttgtcctgct tggcaaggtc gctacgacct cgcccggtgc
     3541 tgtgtgccga cttcagcgtc gacagcaata aaaaa
//
LOCUS       DAOQRM010000221         3476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_30227_length_3476_cov_15.878690, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000221 DAOQRM010000000
VERSION     DAOQRM010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30227_length_3476_cov_15.878690"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PKK81_12570"
     CDS             <1..1069
                     /locus_tag="PKK81_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNV33766.1"
                     /translation="GAILRVPCWPWGFFYLRAVRIVSSRRFAAACIGVVASVLLACLA
                     FAFGETQEDHTVMKRRQQGILTGMPFMANITPRTFIDDAGRKLYVAKAPTRVVSLAPS
                     ITEMLFALGLDEQIVGVTEFCDFPAAAKAKSKVGYANPNLESLVALRPELIIAPREFH
                     RANVLAKLEELKIPVLLLEATSVENIFSHIHTLGRIFDRSTEAHAMTAAMRSRMAEIS
                     SRTEHLPRIRVLYVINSQPLITVGPGSYIHQMIGLAGGTNIASGAATPYPRLTMETVL
                     KEDPEVLIFPMGSVETVPRREQQEWRRWTTLTAVQQNRLREVSANALNRPGPRVMEGL
                     EELVRVIHPEAFPPESVPVQP"
     gene            1156..2226
                     /locus_tag="PKK81_12575"
     CDS             1156..2226
                     /locus_tag="PKK81_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNV33767.1"
                     /translation="MTGHERPFQGAILTPVRWCVIMGSLSVVALILAVVCLQLGTQYI
                     GLGKLISVLSSALLDQPTDNDVLKTTSVILLQVRLPRVFLGFLVGVCLASVGVALQAL
                     LRNPLADPYVLGVSSGAALGVAVAVLFGIGTTVLAFSLLPVCGFLGGLVALLVVYRMA
                     ATYDRLPIHSVLLAGVILNAIFSALIMFITSIMEPNRSFGMMAWLMGSLTAPAYPALA
                     ALSVYLLAGLFLLFKQVRVLNILALGEEPARSLGIDTERAKRVIFLVSALLTGAVVSV
                     SGMIGFIGMIIPHAVRLVIGADHRLLLPASALVGGIFLMVADTMARTFFVPSEVPVGI
                     ITALAGGPFFVYLLVWRKDRLV"
     gene            2223..3119
                     /locus_tag="PKK81_12580"
     CDS             2223..3119
                     /locus_tag="PKK81_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV33768.1"
                     /translation="MRLPVDPMCTADVAAGVYSDRHHAYDVQGASFCYGKLSGRESRW
                     VLSDVSLHVEPGEILGIVGPNGSGKTSLVKLLAKLAVPQRGTLSLFGRNLADLSREET
                     ARTVAFVPQESPPAFSFTVAETVLMGRYPHRRQTRWSLGFGWEDREDCAAAAQAMATM
                     DIGHLASRAVTDLSGGERQRTMIARALAQTPRVLLLDEPTAFLDLQHQLEICSVLRRL
                     TDEQGLTVVIVSHDLNLASQYCDRIVMLKEGSMYSMGTPAEVLSVEALRAVYGCEVLI
                     DPHPESGLPRITLPRQAVPFRG"
     regulatory      3183..3445
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          596 a         1004 c         1107 g          769 t
ORIGIN      
        1 gggtgcaatc ctcagggttc catgttggcc ctggggattt ttttatttgc gcgccgttcg
       61 aatcgtctcc agtcgccggt tcgcagccgc atgcatcggc gtggtcgcgt ctgtgctgct
      121 ggcctgcctt gccttcgcat tcggagagac ccaggaagat cacacggtga tgaaacgtcg
      181 tcagcaaggc atccttacgg gcatgccgtt catggccaac attaccccgc gcaccttcat
      241 cgacgatgcg ggcagaaaac tctatgtcgc caaggcgccc acacgggtcg tgtcattggc
      301 gccgagcatc acggagatgt tgtttgcgtt ggggctggac gaacagatcg tcggggtcac
      361 ggagttttgc gatttcccgg cagcagccaa ggctaaatcc aaggtcgggt acgccaaccc
      421 caatcttgaa tccctcgtgg ccctccggcc ggaattgatc attgcgccgc gggagttcca
      481 ccgcgcgaat gtgttggcca agctggagga attgaagatt ccggtgctcc tgctggaagc
      541 cacttcggtg gagaacattt tttcgcacat tcacacgctg ggacgcattt tcgatcgttc
      601 gacggaagcc catgccatga ccgcggccat gcggagtcga atggccgaga tcagcagtcg
      661 aaccgagcac ctgccgcgca tccgcgtgct gtatgtgatc aacagccagc ccttgatcac
      721 ggttggtccc ggcagttaca tccaccagat gatcggcctg gccgggggaa ccaatatcgc
      781 ctccggagct gcaacaccct atccacggct gaccatggaa acggtcctga aagaagatcc
      841 cgaagtgctg atcttcccaa tggggtcggt tgaaacggtg cctcgacgcg aacagcagga
      901 gtggcggcgc tggacgaccc tgaccgctgt gcagcagaac cggttgcgcg aggtttcggc
      961 caatgcgctc aatcgtcccg ggcctcgtgt gatggaaggg ttggaagaac tcgtgagggt
     1021 gattcacccc gaggcctttc ctcccgaatc ggtaccggtc cagccatgat gccttcggtc
     1081 ggccactcca cgtcacctgc tcccgcattg gcctcgcccc cgttgccttc cacgagccgt
     1141 cacagttccg ctccaatgac cggacatgaa cggccgtttc agggggccat cctgactccg
     1201 gtgcgttggt gcgtcatcat gggcagtttg tcggtcgtcg cgctgatcct ggctgtcgtc
     1261 tgtctgcaac tcgggacaca gtacatcgga ctcgggaaac ttatcagcgt gctgtcgtcc
     1321 gcgctgctcg accagcctac cgacaacgac gtgctcaaga cgaccagcgt catcctactg
     1381 caggtgcgtc tgccgcgagt ctttctggga tttctggtgg gagtctgcct ggcatcggtc
     1441 ggggtggcgc tgcaagccct gctcagaaat ccactggccg atccctatgt gctcggggtg
     1501 tccagtggag ccgcgctggg tgtggctgtc gcggtgctgt tcgggatcgg gactacggtt
     1561 ctcgccttct cgctcctgcc ggtctgcggg tttttaggcg gactggtggc cctgctggtg
     1621 gtctatcgga tggcggccac ctacgaccgg cttccgattc attccgtgct gctggccggt
     1681 gtgatcctga acgcaatttt ctcggcgctg atcatgttca tcacctcgat tatggaaccg
     1741 aatcgttcct tcggcatgat ggcctggctc atgggatcgc tcacggcccc ggcctatccc
     1801 gcgttggctg cgctctcagt ctatctgttg gccggtctct tcctgctttt caagcaggtg
     1861 cgggtcctga acatcctggc cttgggtgaa gagccggcac ggtcgttggg aatcgatacc
     1921 gaacgcgcca aacgggtgat ctttctcgtg tcggcgctgc tcaccggcgc cgtggtgtcc
     1981 gtcagcggca tgatcggatt catcgggatg atcattcctc acgcggtgcg cctggtgatc
     2041 ggggcggatc atcgattgct cctgccggct tcagcgctcg tgggcggcat atttttgatg
     2101 gtggccgata ccatggccag gacattcttc gtgccgtcgg aagtcccggt cggaatcatc
     2161 acggccctcg cgggcggccc gttctttgtc tacctgctgg tctggcgaaa ggatcggctc
     2221 gtatgaggct tcctgtagac ccgatgtgta cggctgatgt tgcggctggt gtctactcgg
     2281 accggcacca tgcctacgat gtgcaggggg cctcgttctg ctatggcaag ttgtctgggc
     2341 gggaaagccg gtgggtcctc agcgatgtga gcctgcacgt cgagccaggc gaaatactcg
     2401 gtatcgtcgg gcccaatgga tcgggcaaga cgtcactcgt gaaactcctg gccaagcttg
     2461 ccgtgcctca acggggaacc ctctcgctct tcggaagaaa cctggctgac ctttcacgag
     2521 aggagacggc gcggaccgtg gcgttcgtcc cgcaggaaag tccgccggct ttctccttta
     2581 cggtggcgga aacggtgttg atgggacggt atccgcatcg ccgacagacc aggtggagtc
     2641 tcgggttcgg ctgggaagat cgggaggatt gtgcggcggc cgctcaggcc atggccacga
     2701 tggacatcgg ccatctggct tcgcgtgcgg tgaccgacct gtccggcggt gaacgtcaac
     2761 gtacgatgat cgcgcgggcc ttggcccaaa cgccccgggt cctcttgctc gatgagccga
     2821 ccgcctttct cgatcttcag catcaactcg aaatttgctc ggtgctgcgt cgtttgaccg
     2881 acgagcaggg cctgaccgtc gtcatcgtgt cgcacgattt gaacctggcc agccagtatt
     2941 gcgaccggat cgtcatgctg aaggaagggt cgatgtattc gatgggcacg ccggctgagg
     3001 tgttgtcggt ggaggcattg cgggcggtct atggctgtga ggtcttgatc gatccgcatc
     3061 cggagtcggg actgcccagg attaccttgc cgaggcaggc tgtgccgttc aggggatgat
     3121 cacgtgttgc gggatgggtt cgtgcccggc ccacccgtgt gaagagaagg ggcgcgtcat
     3181 cattggagag cggagtgtca ggaagacgcc gtcagcgtcg gggaagatgg tgcaaaacca
     3241 tcacggtgcc gccactgtaa gcggggagtg actctgcatt gagccactgg cgagtggcgt
     3301 ggttcgtgaa tcgtatctcg tgaagcgcag gaggttctct ccgaccgttt cacgaacggc
     3361 gttttacgtt tcacgctcag ctgggaaggc gcagagaaac gatgagccgc aagtcaggag
     3421 acccaactga cgggtttgta actgaaccct tcgagcgaaa gggagtgtca gtatgt
//
LOCUS       DAOQRM010000222         3458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_30478_length_3458_cov_19.223038, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000222 DAOQRM010000000
VERSION     DAOQRM010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30478_length_3458_cov_19.223038"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PKK81_12585"
     CDS             <1..872
                     /locus_tag="PKK81_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNV33769.1"
                     /translation="FVHADIGDRQAVHAALQTHRIEGIINCAAETHVDRSILDPGAFA
                     RTDVVGTGILLEEARQAGVQRFLQVSTDEVYGSVEQGSSTEGDRLEPRSPYSASKAGG
                     DLLVLSYWTTYRFPVVVTRGSNTYGPNQYPEKFIPLFATNAIDGEPLPLYGDGRQCRD
                     WLSVYDHAAGIQHVFEQGEPGTVYNVGGGNERENITVAEQIVATLGKSRSLIRFVQDR
                     PGHDRRYSIDCRRLRALGWSPQVPFEEGLKQTVEWYRTHESWWRTIKSGEFKDYYQQQ
                     YAKRLQQGTACGL"
     gene            860..1711
                     /gene="rfbD"
                     /locus_tag="PKK81_12590"
     CDS             860..1711
                     /gene="rfbD"
                     /locus_tag="PKK81_12590"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNV33770.1"
                     /translation="MRIVITGAQGQLGTDLRQVLQGHQLTLLDLPTFDLTHQDCGRVI
                     VEAAPEVVIHAGAHTDVDGAERNPALALAVNAEGTERVARAAALVGARLIYISTDYVF
                     DGRGTRPYVETDPTNPVSAYGESKRAGEQRALACCENTLVVRTAWLYGLHGRNFVKTM
                     LQLASERPCLKVVADQRGCPTFSGDLAGMIGKLLAHPAQGVLHVTNAGNCTWHEFATE
                     IVRLSGRPVPVEAITTADMPRPAKRPAYSVLSADRLHHLGFTMPSWQDGLQRFMKALP
                     AVSSAVS"
     gene            2242..>3458
                     /locus_tag="PKK81_12595"
     CDS             2242..>3458
                     /locus_tag="PKK81_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNV33771.1"
                     /translation="MTSPPADSALRSVHTDARSVSGHALFWAFQGYVMAAVACSSFFP
                     MLFRYQEHAVIVLVVLSLGMCAIERVSPWIKNPLDLPLWLFMIWVLCTVPFATDSAYS
                     FAEWKKFVAQAGVFYWSLLVLDRCRRENLPQQILWALALTGAALAVYSLVDFVDRGGT
                     WRDRYIRANAFGSDYNWLSTYMVMTIPVVGSLIVLGRLVWFRAAQALALALMGGAQLF
                     SYTRGGWLGHAAQGIAFAAMVGGRRLALWVLGLFAMAGAGLLVASQAGFQRDTVAAST
                     VDTRLTVWAIGLRQVATHPVVGIGYGNNSFVKKFPEYSSEAQAQVSEGTRIIPAMHNT
                     FLMVALGSGLPALLSFVWIFVALLRRLIPIPWRAAWNDRWSVLAAGIGLAVIGFAVRN
                     LFDYMFMGSLAHI"
BASE COUNT          578 a          953 c         1129 g          798 t
ORIGIN      
        1 cctttgtgca tgccgacatc ggtgatcggc aggcggtaca tgccgcgttg cagacgcacc
       61 gcattgaggg catcatcaat tgcgcggcgg aaacgcacgt cgatcggtcg attctcgatc
      121 ccggggcctt tgcccgcacg gatgtcgtgg gtaccggtat tctgttggag gaagcgcgtc
      181 aggccggggt gcaacgattt ctccaggtca gcaccgacga ggtctacggc agtgtcgaac
      241 agggcagttc cacggaggga gatcggctgg aaccgcggag tccctattcg gcgagcaagg
      301 ccggaggaga tctgctggtg ctgagttatt ggaccacgta ccggtttccg gtcgtggtca
      361 cgcgcggcag caatacttac ggccccaacc agtatccgga aaaattcatt ccgctgtttg
      421 cgaccaacgc gatcgatggg gagccgcttc cgttgtacgg tgacggcagg caatgccggg
      481 actggttgtc ggtctacgac catgcggcag ggattcagca tgtgttcgaa cagggggagc
      541 cggggaccgt ctacaatgtc gggggcggca atgagcggga gaatatcacg gtggccgaac
      601 agatcgtggc gacgctgggg aagtcccgct cgctgattcg ttttgtgcag gaccgcccgg
      661 ggcacgatcg tcgctattcg atcgattgtc gccgcctgcg cgccttgggt tggtcgccgc
      721 aagtgccctt cgaggagggg ctgaagcaga ccgtcgagtg gtatcgcacg catgagagct
      781 ggtggcggac gatcaagtcc ggagagttca aagactatta tcaacagcag tatgcgaagc
      841 ggttgcagca ggggacggcg tgcggattgt aatcaccggg gcgcagggac agttgggcac
      901 ggatctccgg caggtcttgc aggggcatca actgacgttg ctcgatctcc cgacgttcga
      961 cctgacgcat caggactgcg gccgtgtgat tgtcgaagcc gcgcccgagg tcgtgattca
     1021 cgccggagcg catacggatg tggatggggc agaacggaat ccggccctgg cgctggccgt
     1081 gaatgccgag gggaccgagc gagtggcccg tgcggccgct ctggtcggag cgaggctcat
     1141 ctatatttcc actgattatg tgttcgacgg ccggggcacg cgtccctatg tcgaaaccga
     1201 tccgaccaat cccgtcagcg cctatggcga gtcgaaacgg gcgggtgagc agcgggcctt
     1261 ggcctgttgt gagaataccc tggtggtgcg taccgcctgg ctctatggcc tgcacggccg
     1321 gaatttcgtc aagaccatgt tgcagctggc ttccgaacga ccctgtttga aagttgtagc
     1381 cgatcagcgg ggttgcccca ctttttccgg agatcttgcc ggcatgatcg gaaagctcct
     1441 ggcgcatccg gcgcagggtg tattgcacgt gacaaatgcc gggaactgta cctggcatga
     1501 attcgcgacg gagattgtcc gcttgtccgg gcgccccgtg ccggttgagg cgatcacgac
     1561 ggccgacatg ccgagaccgg cgaagcgacc ggcctattcg gtcttatcgg cagaccgtct
     1621 ccatcatctc gggttcacca tgccatcctg gcaggatgga ttgcagcgat tcatgaaggc
     1681 cctgccggca gtgtcctctg cagtttctta gaagtctcgc ttcgtattct cgtttgtatc
     1741 gggattcatc cctctggtcg gacgggtcgt gaagctcgct cattccgctc gccgcattcg
     1801 gttcctcgtt cccacagggc gcttagtcag ggcgcatcgc gatccacggt gcattatttt
     1861 gcgatacctc ctacgctgag tgtgtcagta agacacattt cttgtgcaac ccccgcgatt
     1921 tctctatact gccgtcctgc agagccctcg tagcgtttct cgtgacgatg cccggtgaac
     1981 gtagccggtg agttcgagcc tgtgtgcgcg cctgctccgt cagaccggta aacgggtgat
     2041 ccacttcacg catgaatacc gacatgccga tccgatccca ccgggggccg agagcggggt
     2101 gttcttgtgc atgaggggcg attgtgcctt ctgcgcgtcg ttcgttgcgg tgtatgagcg
     2161 tccggcctga gagggtgaag tgtatggatg tagttcgttg tgcctctttc tgtgacacgc
     2221 gactctgatg gaacgaatgt tgtgacgtcg ccgcccgccg attccgctct ccggtccgtt
     2281 cacacggacg cgcgatccgt ttccggccat gcactcttct gggcattcca agggtatgtg
     2341 atggctgcgg tcgcctgttc gagttttttc ccgatgctgt tccgctatca ggaacatgcc
     2401 gtcatcgtgc tcgtcgtact ttcgctaggg atgtgcgcga tcgagcgggt gagtccctgg
     2461 atcaaaaatc cgctggatct tcccctgtgg ctcttcatga tctgggtgct ctgcaccgtt
     2521 cccttcgcga ccgactccgc atacagtttt gccgaatgga aaaaattcgt agcgcaggcc
     2581 ggggtctttt attggtcctt gctcgtgctg gatcgctgtc gacgggagaa cttgccgcag
     2641 cagattctct gggcgctggc actgaccgga gcggcgcttg ccgtctattc gttggttgat
     2701 tttgtcgaca ggggcggcac gtggagagac cggtatatcc gggccaatgc gttcgggtcg
     2761 gattataact ggctcagtac ttacatggtg atgacgattc ccgtggtcgg cagcctcatc
     2821 gtcctgggtc gcctggtctg gtttcgggcg gcacaggcct tggcgcttgc gttgatggga
     2881 ggcgcgcagc ttttttccta tacacgtggc ggatggcttg gtcatgcggc tcaaggcatc
     2941 gcatttgcgg cgatggtggg tggccggcgt ctggctttgt gggtgctggg actcttcgcg
     3001 atggcgggag cggggctgct ggtcgcgtcg caggccggat ttcagcgcga tacggtggcc
     3061 gccagcactg tggacaccag gttgacggtc tgggccatcg gattgcggca ggtggcgacg
     3121 caccccgtgg tcgggattgg atacgggaat aattcgttcg tgaaaaaatt ccctgaatac
     3181 tcgtcggagg cacaggcaca ggtgtctgaa gggactcgca tcattccggc catgcacaac
     3241 acttttctca tggttgcgct ggggagcggc cttcccgcgt tactcagctt tgtctggatc
     3301 tttgtcgctc tcctgcgccg gctcattccg attccctggc gggctgcatg gaacgatagg
     3361 tggtcggtgc tggctgcggg tatcgggctg gccgtcatcg gcttcgccgt acgcaatctc
     3421 ttcgactata tgtttatggg cagcctggcg cacatatt
//
LOCUS       DAOQRM010000223         3456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_30500_length_3456_cov_13.371949, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000223 DAOQRM010000000
VERSION     DAOQRM010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30500_length_3456_cov_13.371949"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..>3456
                     /locus_tag="PKK81_12600"
     CDS             221..>3456
                     /locus_tag="PKK81_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV33772.1"
                     /translation="MLSAPPQSPAPLGVSGRLAALFVGSKLTPLIIIASLLLGLFAIV
                     LTPREEEPQIVVPMADVWLSFPGASAKVVEEQLTKPIERKLSEIKTVEYVYSISRPGG
                     ALIIVRFYVGEPMEQSLVDLYDKLMSNQDLLPPGAQPFQVKPRDVNDVPIVTLTLSSE
                     RYTDFQLRQLGDQVLEDAKKVPGTAAGFVVGGRGRELRVQIDPSRLKAYGLTPLRVAG
                     VIQGENLALPTGRFESRNESFLVETGRFIRSKEDLEGVVVGVNEQRPVYLRQVAEVTD
                     GPTEATSYVWHGEGPGGAHETLHVKGETNTPASHLLPFTSHENQAVTVAIAKQAGTNA
                     VTIAQGVIRKVEELKGTRIPDEVRVTVTRDYGETADEKANELLWHLLIAVVAVVVFLG
                     VTLGLRPALVVSIAIPLTLALTLFISMLIGYSINRVTLFALIFSIGILVDDAIVVVEN
                     TYRHLTLRIRPHREASLFAVDEVGNPTILATLTVIAALLPMAFVSGLMGPYMRPIPVN
                     ASIAMFVSLLVAFIVIPWFCQTCYRPGVHMAGVDHESFEGSRSYRLYQRLLAPVLSRP
                     VVAYLVLAVIALLLVGSVALFYTRAVVVKMLPFDNKSELQLVIDMPEGTTLEETARVT
                     KALTQYVRTIPETRDYQAYVGTASPFNFNGLVRHYYLRDQPYEADIQINLVAKEQRKT
                     QSHDIAQRIRSAVQDIGRPFGANVKVLEVPPGPPVQSVLVAEIYGPDYERQRAIAREV
                     RGLFETTAGVVDVDDYLEADQVKYVFSVDRAKAALAGIPSEEIVRTLRLALEGADVGL
                     VHIPKENRPVQVRLRLPVIERTGLEHLGEISLRTTAGHMVPLSELIRVEQTVRDTAIY
                     HKNQKPVVYVIADVGGVGPDKGESPVYGVMGIGKKLEQLVLPEGYAMTQQYAVQPWSE
                     QRFAMKWDGEWHITYETFRDMGIAFAVALLLIYLLIVAQFQSFLTPVVIMAPIPLTLI
                     GILPGHWLTGSYFTATSMIGFIALAGIIVRNSILLVDFIQHQEAEGVSLLEAVIRAGA
                     IRTRPILLTAAALMVGAFVIILDPIFQGLAVSLLFGVGASTVLTLVVIP"
BASE COUNT          631 a          988 c         1105 g          732 t
ORIGIN      
        1 ggggttgaag tcctctccgg cgtggacgcc ggtgagcgag tcatcgtgga gggggccaag
       61 ggcagggacg gcgcgttggt acaggatatg acggcagtgg catcgccgcc tcagggatcg
      121 tgactcgccc aaagagccta tagctcatgg cgtatggcag gatcgaaaac aggctgatct
      181 gtcgaatggc gtcaaacccc atccatacgc tataagccat atgctatcag ctcccccaca
      241 gtccccagct ccccttggag taagcggccg cctcgcggcg ctcttcgtcg gcagcaaact
      301 caccccattg atcatcattg ccagcctcct gctcgggctg tttgccattg tcctgacccc
      361 gcgcgaagaa gagccgcaga ttgtcgtgcc tatggccgac gtctggctct catttcccgg
      421 ggcctccgcg aaggtggtcg aagagcagct caccaagccc attgagcgca agctgtccga
      481 aatcaagacc gtcgaatatg tctactccat ctcccgtccg ggcggggcgt tgatcatcgt
      541 ccgcttttat gtcggcgagc cgatggaaca gagtctggtg gatctctacg acaaattgat
      601 gtcgaaccag gacctgcttc cgcccggcgc tcagccgttt caagtcaaac cgagggatgt
      661 gaacgatgtg ccgatcgtca ccctgaccct gtccagtgaa cggtatacgg atttccagct
      721 acggcaactg ggcgatcagg tcttggaaga tgcgaagaag gtccccggca ctgcagctgg
      781 cttcgtggtg ggcggtcgcg gccgcgagtt gcgtgtgcag atcgatccat cgcgcctgaa
      841 ggcctatggc ctgacgccgt tgcgggtcgc cggcgtgatt cagggcgaaa atctcgcgtt
      901 gcccaccggc cggttcgaaa gccggaatga aagttttctg gtggagaccg gccggttcat
      961 ccgctcgaaa gaggacctgg agggagtggt cgtcggcgtc aacgaacaac ggccggtgta
     1021 ccttcgccag gtggccgagg tgaccgatgg cccgaccgaa gcgaccagtt atgtatggca
     1081 tggcgaggga cccggcggtg cgcatgagac gttacacgtg aaaggtgaaa cgaatactcc
     1141 cgcgtctcac cttctacctt tcacgtctca cgaaaatcag gctgtgaccg tggcgattgc
     1201 taaacaggcc ggcaccaacg ccgtgacgat tgcgcagggc gtcattcgaa aagtcgagga
     1261 actcaaagga acccggattc ccgacgaggt gcgggtgacg gtgacgcggg attacggcga
     1321 gaccgccgat gaaaaagcca acgagctgct gtggcatctc ctgatcgccg tggtcgccgt
     1381 cgtggtgttt ctcggagtga cactggggct gcgccccgca ctggtggtct caatcgccat
     1441 tccactcacg ctcgccctga ccctgttcat ttccatgctc atcggctaca gcatcaaccg
     1501 cgtgacgctg tttgcgttga tcttttccat cggcattctc gtggatgacg ccatcgtggt
     1561 ggtggagaac acctaccggc atctcacgtt gcggattcgc ccgcaccgcg aagcctcgct
     1621 gtttgccgtc gacgaggtcg gcaacccgac gattctggcc accctgacgg tgattgccgc
     1681 gctcctgccc atggcgtttg tctcgggctt gatggggccc tacatgcggc ccatcccggt
     1741 gaatgcgtcg attgccatgt tcgtgtccct gctggtcgcg ttcatcgtca ttccctggtt
     1801 ctgccagacc tgttaccggc ccggtgtgca catggccggg gtcgatcacg aatcgttcga
     1861 gggcagccgc agttatcgcc tgtaccagcg gctcctggct ccggtgttgt cccgtccggt
     1921 cgtggcctac ctggtgttgg cggtcattgc gctcttgctg gtcgggtcgg ttgcgctgtt
     1981 ctatacccgg gcggtggtgg tgaagatgct accgttcgac aacaagagcg agctgcagct
     2041 ggtgatcgat atgccggagg gcacgacgct tgaggaaaca gcccgggtga cgaaggcttt
     2101 gacgcagtac gtgcgaacga ttccggagac ccgcgattac caggcctatg tcggcacggc
     2161 ctcgccgttc aatttcaacg gactggttcg gcactactat ctgcgcgacc aaccgtacga
     2221 ggccgacatt caaatcaatc tggtcgcgaa ggagcagcgg aagacgcaaa gtcatgacat
     2281 cgcgcaacgg attcgttccg cggtgcagga catcgggcgg cccttcggag cgaatgtgaa
     2341 agtgctggag gtgccacccg gcccgccggt gcaatctgtg ctggtggcgg agatctatgg
     2401 gcccgactac gagcggcaga gggcgattgc gcgagaggtc cgcggactgt tcgagacgac
     2461 ggccggcgtc gtcgatgtcg atgactacct cgaagccgac caggtgaagt atgtgttcag
     2521 cgtggatcgc gcgaaggcgg cgctggccgg gattccgtcg gaggaaatcg tgcggacgtt
     2581 acgactggca ttggaaggcg ccgatgtggg gctggtgcac attcccaaag agaatcggcc
     2641 ggtgcaggtg cgactgcgcc tgccggtcat cgaacggacc ggtttggagc atctcgggga
     2701 gatttcgttg cgaaccactg ccggccacat ggtgccgttg tctgagctga ttcgcgtgga
     2761 acagaccgtg cgggatacag ccatctatca caagaaccaa aagccggtgg tctatgtcat
     2821 tgcggatgtg ggaggcgtgg gcccggacaa aggcgagagt ccggtctatg gtgtgatggg
     2881 catcgggaag aagctggagc agctggtgtt gcctgaaggg tatgcgatga cccagcagta
     2941 tgccgtgcag ccctggtcgg aacagcggtt tgcgatgaag tgggacggcg aatggcacat
     3001 cacctatgaa acatttcgag atatggggat cgcctttgcg gtggcgctgc tgttgatcta
     3061 tttgctgatc gtggcgcagt tccaatcgtt tctcacgccg gtggtgatca tggcgcccat
     3121 tccgctcacg ctcatcggca ttcttcccgg tcactggctc accggctcct atttcacggc
     3181 cacgtcgatg atcgggttta tcgccctcgc cggtatcatc gtgcgcaatt cgattctgct
     3241 ggtcgatttc attcaacatc aggaggcgga gggggtctcg ctgttggagg ccgtgattcg
     3301 cgcgggcgcg atccggacgc gtcccattct gttgacggcc gcggcattga tggtcggggc
     3361 gttcgtgatt attctcgacc cgattttcca aggactggcc gtctcgctgt tgttcggcgt
     3421 cggcgcctcg acggttctga cgctggtcgt cattcc
//
LOCUS       DAOQRM010000224         3443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_30692_length_3443_cov_18.240260, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000224 DAOQRM010000000
VERSION     DAOQRM010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30692_length_3443_cov_18.240260"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1060)
                     /locus_tag="PKK81_12605"
     CDS             complement(185..1060)
                     /locus_tag="PKK81_12605"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNV33773.1"
                     /translation="MMNWGPELAIILGIIEGLTEFLPVSSTGHLILVGHALGFTGDIA
                     SNVEISIQLGSILAIIAYERTKLASLASHAFREQRDFRSLVAARGNASWATLIQQSIQ
                     AQPNLWFVIGLGLAFLPAALVGFLAHKSIKAYLFSPTTVAISLIVGGIIILVVERMRD
                     RVRVKELLQVTPRLAIWVGIAQCASLIPGMSRSGSTIVGGLLAGLDRRVATEYSFFLA
                     LPTMIIATIYQMLKSQAAFSQDDYVALGIGLVVSFAVAWAVIAAFLTYVQRHSLSVFA
                     YYRMVLGVIVLLVVR"
     gene            1315..2043
                     /locus_tag="PKK81_12610"
     CDS             1315..2043
                     /locus_tag="PKK81_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33774.1"
                     /translation="MRHVWMAGLALMSAMAVSGCVSDKKYHEALADADTVRGDLERAR
                     AQKNALEQQVKTLKDLNAKFGSESQIARDELQRIQHGRDKERDTIDVRTKELEDKVKQ
                     LSAQNRNIKQEYDEARRHNEQLKSLVARYQKEMKERSRSLSSSAPAELTAPLSGGASA
                     AKSASSPTPFGGPVEAAAPPVSLLNINKAPSTDLVLTLGVSQDVADRIVSNRPYRVKG
                     ELVAKNVVPKDIFDDIKDRITVSP"
     gene            2233..>3443
                     /locus_tag="PKK81_12615"
     CDS             2233..>3443
                     /locus_tag="PKK81_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV33775.1"
                     /translation="MRRIGVIVGVLALGLAIAGYVFFNGERKAVVRYRTVPIERGTIV
                     SLVTATGTINPITTIQVGSQVSGMIESLHADFNSKVTANQVVARIDPFPYQARRDQAV
                     ASLANAKAAFDKARIDLAQRRRELDRAKSLIGQQFISQNEVDVALTASEGAVAQLKVT
                     EAAVKQAEAMLQAAELDLKYTVIRSPVDGVVISRLVEVGQRIAASFTIPMLFLIAEDV
                     TKMQVDTNVSEADIGGIAEGKAASFTVDAYPGEQFQGRVRQVRNAPINIQNVVTYDVV
                     VEFENPAFRLKPGMTANVSIVVAKKENILKVPNSALRFTPPKTVRDEQASGRPTKGEG
                     GGRMSAGGTSDAATRQLAIWKQGADEALERVPVEMGISDGAYAEISSASINEGDQVII
                     GIDSPRGDRKSG"
BASE COUNT          730 a          919 c         1137 g          657 t
ORIGIN      
        1 atgaccgcgt tcggttcacc gattcccacc agatattgct tggcgaggac gagggccttc
       61 tcctgacttg tggtcatcac gtcgaagtgg agtttgccgt tctttccttt gacccaggtg
      121 tcgtagacgt ggacgctgtc catttggttt gccatggtca ctcggctcct tggtatgagt
      181 gtagttagcg gacgaccagc agcacgatga cgcccaacac catacggtag taggcgaaga
      241 cgctcaggct gtggcgttgc acataggtga gaaaggcggc gatgacggcc caggccacgg
      301 cgaacgagac caccaggccg atgccgagcg cgacgtaatc gtcctggctg aatgcggctt
      361 gggatttcag catctggtag atggtggcga tgatcatcgt cggcagtgcc aggaagaagg
      421 aatattccgt ggcgacgcgg cggtcgaggc cggctagcaa tccgccgaca atcgtggagc
      481 cggaacggga catgccggga atcagggagg cgcattgagc aatcccgacc cagatggcca
      541 agcggggggt gacttggagc agttccttca cgcgcacgcg gtcccgcatg cgttcgacca
      601 ccaggatgat gatgccgccc acgatcagcg aaatcgccac ggtcgtcggc gagaaaagat
      661 aggccttgat cgacttatgg gcgaggaatc ccacgagtgc tgcgggtaaa aaggctagtc
      721 ctaggccgat gacgaaccag agattgggct gggcttgaat ggactgttga atgagagttg
      781 cccacgaggc attccctcgg gcggccacga gcgaccggaa gtcacgctgc tcacggaatg
      841 cgtgcgaggc cagcgaggcc agcttggtgc gttcgtaggc gatgatggcg agaatggatc
      901 ccagctggat tgagatctcg acgttcgagg cgatgtctcc ggtgaatcca agcgcgtgcc
      961 cgacgagaat gagatggcct gtcgaggaga cgggaagaaa ctccgtcagt ccttcgatga
     1021 tgccgagaat gatcgcgagc tccgggcccc agttcatcat aatagtctga ttaggatggt
     1081 ctgtgagtgt gctggtggtt ggttacgggg ttttgctgaa cggtgattgc atattcccag
     1141 actgctccct gccagtcaag cgtcgtcgac atttgcggag tcgccgcgga ctgcgagcga
     1201 ctcaccggca ggcgcttcgc aatcggcaaa cttgttgaca aagttgagat gatggcatac
     1261 acacaacaag agagaagagg tccgatcggt ccagatcgaa caggggagga tcgcatgagg
     1321 catgtgtgga tggctgggct tgcgctcatg agcgcgatgg ccgtcagcgg gtgcgtaagt
     1381 gacaagaaat atcatgaggc gcttgccgat gccgacaccg ttcggggaga cctggagcgg
     1441 gcgcgggcgc agaagaatgc gctggagcaa caggtcaaga cgctgaagga tctcaacgcc
     1501 aagttcgggt cggaaagcca gattgcccgg gatgaactgc agcggatcca gcatggtcgg
     1561 gacaaggagc gcgataccat tgacgtccgg accaaggaat tggaagacaa ggtcaagcag
     1621 ctgagcgcgc agaatcgaaa catcaagcag gagtatgacg aagctcgccg acacaatgag
     1681 caactgaagt cgttggtcgc gcggtatcaa aaagaaatga aggagcgttc gcgatccctg
     1741 agctcgtccg cccccgcgga attgactgcg cccttgtccg gcggtgcgtc agcagcgaag
     1801 tcggcgtcat ctcccactcc tttcggcgga cctgtcgagg ctgcggctcc gccggtgtcc
     1861 ttgctgaata tcaacaaagc gccgtcgacc gatttggttc tgacgctggg tgtcagccag
     1921 gatgtggccg accggatcgt gagtaatcgc ccgtaccgcg tgaagggtga gctggtggcc
     1981 aagaatgtgg ttccgaagga catcttcgac gatatcaagg accgcatcac ggtcagtccc
     2041 tagtcgtgtc atgaacgtcc gctcatagcg gacagatcgg ttcggaaggc cgtttgccgt
     2101 cacaggcaaa cggccttcgt gttttctgcg ccggcgctgt tctgccaagc caggccgagt
     2161 gtcgcggtac tgcatcgcaa aatcgtttcc tgctagaatg accgcgttgt tcgagggaga
     2221 aggggcctgt ccatgcgtcg gatcggagtc atcgtcggag tccttgcgct gggccttgcc
     2281 atcgccggct atgtgttctt caacggcgag cgaaaggccg ttgtgcgcta tcgcacggtt
     2341 cctatcgaac ggggcaccat cgtctcgctg gtcacggcga cgggaacgat caatcccatc
     2401 accacgattc aggtcggcag tcaggtgtcg ggcatgatcg agagcctcca tgccgatttc
     2461 aattccaagg tcacggcgaa tcaggtggtg gcccgtatcg atccgttccc ttaccaggcc
     2521 agacgcgatc aggcggttgc gagcctggcg aacgccaagg cggcgttcga taaggcccgg
     2581 atcgatctgg cccaacggcg ccgtgaactg gaccgggcca agtcgctgat cggccagcaa
     2641 ttcatctcgc agaacgaagt ggacgtcgcg ttgaccgcct cagaaggcgc ggtggcgcag
     2701 ctcaaagtca cggaggctgc cgtcaagcag gcggaagcga tgttgcaggc ggccgagttg
     2761 gatttgaagt acacggtgat ccgttcaccg gtcgacggcg tggtgatctc gcgcctcgtt
     2821 gaggtcgggc agcggatcgc agccagcttt accatcccta tgctcttttt gatcgccgag
     2881 gatgtcacga agatgcaggt cgataccaac gtcagcgaag ccgacatcgg cgggatcgcc
     2941 gagggaaaag cggcctcgtt cacggtggac gcctatcctg gggaacagtt tcagggtcgg
     3001 gtgcggcagg tgagaaatgc gccgatcaac atccagaatg tggtgaccta cgatgttgtg
     3061 gtcgaattcg agaacccggc gtttcgcctg aagccgggca tgacggcgaa cgtctccatt
     3121 gtcgtggcga agaaagagaa cattctcaaa gtgccgaatt cggccctgcg ctttaccccg
     3181 cccaagacgg tgcgcgatga gcaggcttcg ggcagaccca ccaagggtga gggcggcggc
     3241 cgtatgtccg caggcgggac atccgacgcg gcgacccgtc agttggccat ttggaagcag
     3301 ggggccgacg aggccttgga gcgagtccct gtcgagatgg gcatttcaga cggggcttac
     3361 gctgaaatca gttcggcttc gatcaatgag ggggaccagg tcatcatcgg gatcgactct
     3421 ccgcgtgggg atcgcaagag cgg
//
LOCUS       DAOQRM010000225         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_30921_length_3425_cov_16.849258, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000225 DAOQRM010000000
VERSION     DAOQRM010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30921_length_3425_cov_16.849258"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKK81_12620"
     CDS             complement(<1..230)
                     /locus_tag="PKK81_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV33776.1"
                     /translation="MEPKVNYILVGAFVVLLGATILGAIFWLGKTDYRGIYDRYYVYT
                     RESVAGLSVDSTVKYRGVDVGRVKQVILNPENA"
     gene            complement(241..1074)
                     /locus_tag="PKK81_12625"
     CDS             complement(241..1074)
                     /locus_tag="PKK81_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV33777.1"
                     /translation="MRMHELPPTLATAAQIGVPVIEVSHVSTRFGTAVVHRDVSLTVV
                     RGEVFAIAGGNGCGKSTLLREIIGLLAPTTGTIRLLGTDSRQLTHSNGSPLHRRFGVM
                     FQHGALFSSMTLAENVAVPLREHTPLSAEAIRDIVALKIALVGLPPDSAIKFPSELSG
                     GMRRRAALARAIVMDPELLFLDEPTAGLDPMIAAGFDDLVLELKRLLGLTVVMVTHDL
                     DSLWRIADRVAVLGEGQVLGLGTMDALARSDDPVIRSYFHGPRGRAAHLNHSGTGLHR
                     D"
     gene            complement(1219..2340)
                     /locus_tag="PKK81_12630"
     CDS             complement(1219..2340)
                     /locus_tag="PKK81_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HNV33778.1"
                     /translation="MANQQQPAGVTWLEGRTLRCRGAWTMPDVAPVERALRGMVWPAR
                     SALVFEASGIEAFDTGGAVVLYRALMEARRQGCEVSIEGLRPDYAQLMNLVSSNWGAI
                     VAEVASRSNGTERFRTSIRNRVAQGARALEFLGESATALVRLVASPGRMRWRSCLNSL
                     RLDGVNALPITGLLTFLIGVVIAYQGAEQLRKFGTNIFIVDLVGISLLREIAPLIAAI
                     LIAGRSGSAYAAQIGTMKVTEELDALQTFGLSPIELLVLPRVLALVVALPLLTAYADV
                     LGVFGGMLIASNQLNVSFTAFLSRFEEAVALRHMLIGLGKAPFFAVIIALVGCYQGFQ
                     IRGGVDDVGRHTTISVVQSIFLVIIFDAICSILLNWWDL"
     gene            complement(2379..>3425)
                     /locus_tag="PKK81_12635"
     CDS             complement(2379..>3425)
                     /locus_tag="PKK81_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33779.1"
                     /translation="AGQIVQGDYFAFGPHVEISGIVNGDLYAAGGEVLIDGVVNGDVI
                     VAGAKVILSGTVAQDARIAGAQVTVSGTIGRNATLGGADVHLTETAKVRDNLIAGGGH
                     VQLAGSVGRDARVGAWKATLSNQIERDVIVAAGSVRLTSKATVGGRLRYWGEAAPSID
                     EEATVRGAITQRPLPEGWSIERARQGLVGIRLMAAFIGFVSTLILGLVLLRVYPVFAR
                     RVTATIRERPTAALGWGTVALVATPVVALSFVVTLFALPIGLLLFALYGATVYLARVY
                     AITYVGQILIRRQEDSSSLAWPFVAGLVLYSVLSLIPVVGGLLTLLTVLFGLGALLVS
                     KKELIATLREQNQV"
BASE COUNT          767 a         1203 c          903 g          552 t
ORIGIN      
        1 gcattctcag ggttgaggat gacttgtttc acacgcccga catccacccc gcgatacttc
       61 acggtcgaat cgacgctgag tccggcgacg gactcacgcg tatagacata ataccgatcg
      121 tagatcccgc gataatcggt tttgcccaac cagaaaatcg caccgaggat cgtggcgccc
      181 agcaggacga cgaacgcgcc gaccaggata tagttcacct tcggttccat ccgccacctc
      241 tcaatcccga tgcagtcccg ttccggaatg attcagatgg gccgcacgcc ctcggggacc
      301 gtggaaatag ctccggataa cgggatcgtc cgaacgggcc agcgcgtcca tcgttcccaa
      361 cccgagcacc tgcccttcac ccagcaccgc cacccgatcc gcaatgcgcc acaacgaatc
      421 cagatcgtgc gtcaccatca caacggtcag acccaacaat cgtttcaact ccagaaccag
      481 atcgtcgaat ccggcggcaa tcatcggatc gagtccggcc gtcggttcgt cgagaaacag
      541 cagctccgga tccatgacga tcgcacgggc cagcgccgcg cgtctcctca tcccgccgct
      601 gagctcactc gggaatttga tcgcgctgtc cggaggcaat ccgaccaggg cgattttgag
      661 cgccacgata tcccgaatcg cttcagcgct caacggcgta tgctcacgaa gcggcaccgc
      721 cacgttttcc gccagggtca tggaactgaa cagggcgcca tgctggaaca tcacgccgaa
      781 ccgccgatgc agcgggcttc cgttcgaatg tgtcagctga cgactgtctg tgcccaacag
      841 acgaatcgtc cccgtcgtcg gagccagcaa tccgatgatt tctcgcagca acgtcgattt
      901 cccgcaaccg ttgccgcccg caatggcgaa cacctcgcca cgcacgaccg tgagactcac
      961 atcccgatgc acgaccgccg tgccgaaccg cgtcgagaca tggctgactt cgatcacggg
     1021 gacgccaatc tgcgctgctg ttgcgagcgt gggaggcaac tcgtgcatac gcatcctcaa
     1081 accttcagct ccacgggatg ttcaaaatgg tttcctgcga ggccgcagca agcaaagagc
     1141 cgaggcgtac ccttgtggta cgttgaggct ctgcgcgacg cgagaacgaa gcagggagcc
     1201 attttgaaca tcctgtagct ataggtccca ccagttgagc aagatgctgc aaatcgcatc
     1261 gaagatgatg acgagaaaaa tactttgcac cacactgatc gtcgtatgcc ggcccacatc
     1321 atccacccca ccgcgaatct ggaacccctg ataacagccg accaacgcga tgatgacagc
     1381 aaagaagggc gccttgccga gaccgatgag catatgtcgt agcgccacgg cctcctcaaa
     1441 gcgcgaaaga aaggcggtga aactcacgtt gagctgattc gacgcgatca acatgccgcc
     1501 gaagacaccc agcacatcgg cataggcggt caggagcggc agcgccacga ccaacgcgag
     1561 gacccgcggc aacaccaaca gctcgatcgg cgacaggccg aaggtttgca gcgcatccag
     1621 ctcctcggtc accttcatcg tgccgatctg cgccgcatag gccgacccgg accgcccggc
     1681 gatcagaatc gccgcaatca acggggcgat ttcccgcaac agcgagatac ccaccagatc
     1741 cacgataaaa atattcgtcc cgaacttccg aagctgctcg gcgccctggt acgcaatcac
     1801 cacgccgatg agaaacgtca gcagcccggt gatcggcaac gcattgaccc cgtccagccg
     1861 gaggctgttc aggcaactgc gccaacgcat gcgaccgggc gacgccacca aacgaaccag
     1921 cgccgtcgca ctctccccga gaaactccag cgcccgggca ccctgggcga cacgatttct
     1981 gatcgacgtc cgaaaccgct cagtcccgtt cgaccgcgag gcgacctcag ccacaatcgc
     2041 gccccaattg gacgacacca ggttcatcag ctgcgcatag tccggccgaa ggccttcgat
     2101 cgagacctcg cagccctggc gtctggcttc catcaaggcg cgatacaaca cgaccgcacc
     2161 gccggtgtcg aacgcctcga tcccgctcgc ctcaaagacc agcgcacttc gagcaggcca
     2221 gaccattcct ctgagcgcac gttcgaccgg cgccacatcg ggcatggtcc aggctccccg
     2281 acaacggagg gtccggccct ccagccacgt cacaccggcc ggctgctgct ggttagccat
     2341 tagttgaccg cgcttcatcc gccgtttcga ctcgcgcctt acacctgatt ctgttcgcgc
     2401 aacgtcgcaa tgagttcttt cttactgacc agcagcgcac ccagcccgaa cagcaccgtc
     2461 aacaacgtca acagccctcc gaccaccgga ataagcgaca gcaccgaata caggaccagc
     2521 cccgccacga agggccaggc aagcgacgac gaatcctcct gccggcgaat caggatttgt
     2581 cccacatagg tgatggcata gactctcgcc agatagaccg tcgcgccata caaggcgaac
     2641 agcaacaggc cgatcggaag tgcgaacaat gtgacgacga aactcagcgc caccacgggc
     2701 gtggcaacca gagccaccgt gccccaaccg agagccgctg tcggacgttc acggatcgtc
     2761 gcggtgaccc gcctggcaaa caccgggtag acccgtaaga gcaccagtcc caggatcaac
     2821 gtcgagacga atccgatgaa tgcagccatg agccggatgc cgacgagccc ctggcgcgcc
     2881 cgttcgatgc tccacccttc gggcaacggc cgttgtgtaa tcgcgccccg aacggtcgcc
     2941 tcttcgtcga ttgagggggc cgcttcaccc cagtacctca gcctgccccc gaccgtggcc
     3001 ttcgaggtga gacgcaccga ccccgccgcc acgatcacat ctcgctcaat ctggttcgag
     3061 agcgtcgctt tccaggcacc gaccctcgca tcccgaccga cagacccggc aagttgcaca
     3121 tggccaccgc cggcgatgag gttgtcccgc acctttgccg tctcggtcag atgcacatcc
     3181 gcgccaccga gggtggcgtt cctcccgatg gttccactca ccgtcacctg cgcaccggca
     3241 attctggcat cctgagccac ggtgccggac agaatcactt tggctcctgc cacgatcaca
     3301 tcgccgttga ccactccatc gatcaacacc tcaccaccgg cggcatagag atcgccgttg
     3361 acgatgccgg aaatttccac atgcgggccg aaggcgaaat agtccccctg cacaatctgc
     3421 cccgc
//
LOCUS       DAOQRM010000226         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_31063_length_3415_cov_15.193750, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000226 DAOQRM010000000
VERSION     DAOQRM010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31063_length_3415_cov_15.193750"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            788..910
                     /locus_tag="PKK81_12640"
     CDS             788..910
                     /locus_tag="PKK81_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33780.1"
                     /translation="MSPLCDEVVDQPFLFASGQIEDIGEETLFYLGHRPGHDLH"
     gene            complement(1114..3252)
                     /gene="ppk1"
                     /locus_tag="PKK81_12645"
     CDS             complement(1114..3252)
                     /gene="ppk1"
                     /locus_tag="PKK81_12645"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNV33781.1"
                     /translation="MSSPQTPSETQPHASTPKARAHRPATSDLSRPEWYLNRELSLLE
                     FNRRVLDLAKDQKVPLLERLKFLCIVSSNLDEFFEVRVAGLKEQVTHGVEQRGADGLT
                     PSELLARIAALTHQLVHEQYVVLNQSLIPQLADQRIRFLKRADWMPSHIRWMRRFFSR
                     ELLPLLSPVGLDPAHPFPKLLNKSLNFLVTLEGTDAFGRPNGTAIVQVPRSLPRVIHL
                     PVRNAAWPHDFAFLSSVIHAFVHQLFPGMHVTGCYQFRVTRNSNLFVDEEDVDDLRRA
                     LEGQLPERRFGDEVRLEVADNCPPDLVYFLREQFHLDARDVYQCHGPVNLHRLMAVPD
                     LVDRPDLKFQPFTPGIPTTPVPSEDWFDAIRQGDILLHHPYQSFAPVTEFLRQAATDP
                     HVLTIKQTLYRTGADSAIVQSLVDAARGGKEVTVVIELRARFDEEANIELAHDLEEAG
                     AHVVYGVVGHKTHAKMSLVLRREGRMLRSYTHLGTGNYHARNARLYTDFGLLTCDAAI
                     GRDVRTVFQQLTSLGRPGRLKHLLQSPFTLHATLLKWIGRETDRAKQGRPAHIIAKMN
                     ALLEPQIIRALYKASQAGVKIDLIVRGPCALRPGIAGLSEHIRVRSIIGRFLEHSRIF
                     YFLNDGDDRVFLSSADWMDRNFFRRIEVAFPVLDKTLRQRVIDEGLRPYLEDNTHAWI
                     LHRDGTYRRQTPGRRAARSSQQWLMNKLVT"
BASE COUNT          666 a          927 c         1117 g          705 t
ORIGIN      
        1 gctgttctcg ttcggcctcc tcgacggact gcaagtacgc ctgcgtcggc ctcacgtgcg
       61 agaagccccg cgggacttcg gtctcgaacg cctcgcgacg gcatgcatga atagtccggg
      121 ctagtcgcga gggcaaccgc tcgtctgaat atcggcttgt acctgttccg gtatacgctg
      181 gatcgttgcg tgccgtcagg tagcctgcta gaggcatcgt gccgggggta ggaagcgtca
      241 ggcctccatg ccgaggcttc tgagctggtt cgtgccgatt gtgccgcgga cactggaggt
      301 tgcggattgc atgcggcaat cgccacggaa tctcgcgtgg ccaatcaccg ctcccgggtc
      361 attccaatct ttcttttcct gattgtttct cccttacaac gatggtgtga tcgggctggg
      421 ctgtaggcgg ccgtgggatc ggccccctat gagcgcggtt tcgggtcaaa atggtgggct
      481 gttccgctcc ggctcagaag aggggaacag cccgggagag aggtgcggag aggtggacgc
      541 agctcaactc ccaatcggta gccggctttc aggatcggta aaccgcttct gcggaggtgt
      601 cgggcctcca tgccgcgcgt cgtaccgaag cccgttcttc agacaattgc ttgcatgtga
      661 aacagagcgt cgcatcaggt tgtgcgcgta gtcgcggcag agggatgtcc gctcggcagc
      721 gatggcagat gccgtacgaa gtgtcgagca tgcggctgag ggcgcgctca atgtgcctgg
      781 gtgtgacatg agtccgttgt gtgatgaggt cgtcgatcag ccgttcctgt tcgcaagcgg
      841 ccagatcgag gatatcggtg aggagacctt gttctacttg gggcacaggc cgggacacga
      901 cctgcattaa gagcatgcgg cggcttcgaa gcaggtgtgt gcgtaacgag gcgaatgctt
      961 ggtggtgtgg atggagttga atgccctgcg cgacgctcat ggtactcccc tcctcgttgt
     1021 cgagcccgca gcatagaggg ctgacgtgac aaccggataa gcaaccggtt aaggaggggt
     1081 taataatgac gagtggttgc gcctaccagg aggttaggtg acgagcttgt tcatgagcca
     1141 ttgctgggac gaacgagcgg ctctgcgtcc cggtgtttga cgccggtagg tgccatcccg
     1201 atggagaatc caggcgtgcg tattatcttc gaggtagggg cggaggccct cgtcgatcac
     1261 gcgttgccgc aatgttttat ccagcacagg aaacgccact tcgatgcgtc gaaagaaatt
     1321 gcgatccatc caatcggcgc tggagagaaa gacccggtcg tcgccgtcgt tcagaaaata
     1381 gaagatccgg ctatgttcga gaaaccgtcc gatgatcgaa cgtacgcgga tatgttccga
     1441 gaggcccgcg atgccgggtc gtaaggcgca gggacctcgg acgatcaagt cgattttcac
     1501 cccggcctga gaggccttgt agagggcccg aatgatttga ggctccagga gcgcattcat
     1561 cttcgcgatg atgtgggctg gtcggccctg cttagccctg tcggtttcac ggccgatcca
     1621 tttcagcaat gtggcatgca gggtaaaggg cgattggaga agatgtttga gccgtcccgg
     1681 ccgtcccagg gaggtgagtt gctgaaacac cgtgcggacg tcccgtccga ttgccgcatc
     1741 gcaggtgagc aatccaaaat cggtataaag ccgtgcattg cgggcgtgat agttgccggt
     1801 tcctagatgc gtatagctcc gcagcatccg gccttcccgc cggagtacga gactcatttt
     1861 ggcgtgggtt ttatgtccga ccacgccgta caccacatgg gcgccggcct cttccagatc
     1921 atgcgccagc tcgatgttcg cctcttcgtc gaaacgggcc cgtagttcga tcaccacggt
     1981 cacttctttt ccgcctcggg ctgcgtccac cagggattgc acgatggcgg aatccgcccc
     2041 tgtgcgatac agggtctgct tgatggtgag gacgtgcggg tccgtggccg cctggcggag
     2101 aaactccgtc accggcgcaa acgattgata gggatgatgc agcagaatgt cgccctgccg
     2161 gatggcatcg aaccagtctt cgctcggcac aggcgtcgtg ggaatgccgg gggtgaaagg
     2221 ctgaaatttt agatcgggcc gatccaccag gtcgggcacg gccatcagtc gatgcagatt
     2281 gaccggtccg tggcattgat acacgtcacg cgcatccaga tgaaactgtt cccgcaggaa
     2341 atagacgagg tcgggcgggc aattgtccgc cacttcaagc cgcacttcgt cgccgaatcg
     2401 ccgttcggga agctgacctt cgagcgcccg ccggaggtcg tccacgtctt cttcgtccac
     2461 gaaaagattg ctgttccgtg tgacgcggaa ttgataacaa ccggtgacgt gcatgccggg
     2521 aaagagctgg tgcacgaacg catggatcac cgacgacaag aacgcaaaat cgtggggcca
     2581 ggcggcgttg cgcaccggca ggtgaatcac gcgcgggagc gaccgcggga cctgcacgat
     2641 cgcggttccg ttggggcggc cgaaggcatc ggtcccttcg agcgtcacca gaaagttgag
     2701 actcttgttg agcagtttgg gaaaaggatg cgcgggatcc aggccaaccg gactcaggag
     2761 cgggagcagt tcgcgcgaaa aaaaccggcg catccaccga atgtgcgacg gcatccaatc
     2821 ggcgcgtttg agaaaacgga tacgttggtc ggccagctga gggatgagtg attgattcag
     2881 gacgacgtat tgctcatgaa ccagctggtg cgtcagcgcg gcgatgcggg ccaggagttc
     2941 cgacggggtc agtccgtcgg cgccccgttg ttcgactcca tgggtcacct gttccttgag
     3001 tcctgccacg cggacttcga agaactcgtc gagattggag ctcacgatgc agagaaactt
     3061 caatcgttcc agcagtggaa ccttctgatc tttcgccaga tcgaggacgc gccggttgaa
     3121 ttccaggagg ctgagctcgc ggttcaggta ccattccggc cgggagaggt cactcgtggc
     3181 cggccggtga gccctcgcct tcggcgtcga tgcgtgcggc tgagtttctg aaggtgtctg
     3241 cggcgatgac ataaccagtg ggtcggcagt gtatctcagc cgtgcgaaac ggcggatcaa
     3301 gaaacggtta aatttttcac catgctgccc ggactatcca ggtggcaacc ggtgtcacag
     3361 ggcgcatccg atgcgagcgg tggagaccac gaagttgtcg aagtatcgct gttga
//
LOCUS       DAOQRM010000227         3354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_31922_length_3354_cov_14.691422, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000227 DAOQRM010000000
VERSION     DAOQRM010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31922_length_3354_cov_14.691422"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PKK81_12650"
     CDS             <1..647
                     /locus_tag="PKK81_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNV33782.1"
                     /translation="CDISGSDLAEFQKIKRTLEYAPHQTVFYEGHLCLGLYLLCSGKV
                     KLTRSSTRGRRQIVRILGPGELIEKHVFGESPFHEVTCETLEPSQVCVIEKERYLAVI
                     HRNPQLAIKLIQLLSNEVGVNMDHLDQFTFKTARERLASLLLELGDRFGKKNDDHVRV
                     GLTLKREEVAEMAGITVETAIRLLGVFRDEGVLTIDGRTITLLNPDRLSRIAER"
     gene            970..1518
                     /locus_tag="PKK81_12655"
     CDS             970..1518
                     /locus_tag="PKK81_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV33783.1"
                     /translation="MVSQGSRRTSLTVDPLALLKREHRMILDRLAMVETAMSSRSSGS
                     GTVKGTNRETLRELLEFFTGPVDVHFKREEMLVGDLRRILGRKQEEQEQFQSFLDEHR
                     ALKAAAAAVMRQLASKRTDAQDAAASKAFGGLRTLTGELHALIRRYRGQIACEERLLF
                     ALAEMRLTAERRRRISRRMLQV"
     gene            1757..2458
                     /locus_tag="PKK81_12660"
     CDS             1757..2458
                     /locus_tag="PKK81_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33784.1"
                     /translation="MMRVRRVVRNIGMWGAGLAIAAVFGVACVQPASAEEGTGNMVFF
                     KGGFMNLNQDRSGQIFTDTAGIAGANGGNAGWYAGAGLDLVMSKDVWGGMDKTWVVGE
                     IGLQFNSINSNRVNNAAGSIATRTLTDTLATDPQKVQLTMVTIDVAPKIKFMEGSAFR
                     PWVIPIGLDIHVISPPSNRTQYLDLGVQFGGGFEYAVWKAFKVGVDARYHLTARLTNT
                     NNDYFQVGPYIGISF"
     gene            2806..3279
                     /locus_tag="PKK81_12665"
     CDS             2806..3279
                     /locus_tag="PKK81_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNV33785.1"
                     /translation="MFALVWIHLLAAMVWIGGMVFLSVVLVPVLKRDGAFAQYALLFR
                     TIAYRFRAVVWGAMGILVATGLTLAAGRSIPLMEPSRWPTIFAAKIGLVTLLFTLTLL
                     HDLVVGPRVRRSLGIPEAERSARDRVLLRYSVLVPRISLLVALLVLLLAVVLART"
BASE COUNT          673 a          924 c         1048 g          709 t
ORIGIN      
        1 tgtgcgatat ttcaggaagc gatctcgccg aatttcaaaa aattaaacgc acgctcgagt
       61 acgcgcctca ccagaccgtc ttctacgaag gtcatctgtg cttgggtctc tacctgttat
      121 gttcgggtaa ggtcaagctc actcgctctt cgacgcgagg ccggcggcag atcgtccgca
      181 tcttgggccc tggtgagttg atcgagaaac acgtcttcgg tgagagcccc tttcatgaag
      241 tgacgtgcga gacattggag ccctctcaag tctgcgtcat cgagaaggaa cgctacctcg
      301 ctgtcatcca cagaaatcct caactggcga tcaagctgat ccagctcctc agtaacgaag
      361 tcggggtcaa tatggatcac cttgatcaat tcacgttcaa aactgcccgc gagcgattag
      421 ctagcctgct gctggaactc ggcgaccgat tcggcaaaaa aaatgacgat catgtccggg
      481 tgggactcac gctcaaacgg gaagaagtgg ccgagatggc cggtattacc gttgaaaccg
      541 ccatccgcct gctgggcgtc tttcgcgacg agggcgtact caccatcgat ggaagaacca
      601 tcaccttgct gaatccagac cgcctctcca gaattgccga gcgctgaact aaacccgtcg
      661 accgtaattc gtgaagcgtc gttcgtgaag tccgacaggc ggaaagcgac gttcacgcct
      721 cgagggttca catcccgcac cgcttgagtc cttctccctt tcgtcgttct cgtacgcgcc
      781 actcatctcg atgttcgccg catctctgca gacataggtc caatggccta tccgaaacct
      841 tgagataaat catgtatcag actggccgat gccagtgtgt gctcgcctac ctgttcggta
      901 cagtgcgcac ggcatgacgc tgtcgcattg ctcgcatgtt ggagcttccg tccatgggat
      961 cccgaaacga tggtgtctca aggtagcagg cgaacgagtc tcaccgtcga tccgttggcg
     1021 ctgctcaaac gggagcaccg aatgattctg gaccgactgg ccatggtcga aacggcgatg
     1081 agttctcgct catccgggag cggtacggtc aagggaacga accgggaaac actacgagaa
     1141 ctgctcgaat tctttaccgg ccccgtggac gtgcatttca aacgggaaga aatgctggtg
     1201 ggcgatctcc ggcgaatcct gggccggaag caggaggagc aggagcagtt ccagagcttc
     1261 ttggacgaac accgggcgtt aaaagccgcc gcggccgcag tgatgagaca gctggcgagc
     1321 aagcggaccg atgctcaaga cgcagcggcg tcaaaagcat tcggcggatt gcggacgctg
     1381 accggggagc ttcacgcgct gattcgtcgc taccgtggac agatcgcctg cgaggagcgg
     1441 ctgctgtttg ccttggccga gatgcggttg accgcagaac gaagacggcg aatcagccgg
     1501 cgcatgttgc aagtgtgaag tcgcgcatga gcggcaacgg gctcgggact gatgaggcgc
     1561 gcgactcgcg gtgtgagacc ggcacgagtg tcgccgatgc gtcggctcga agtggggatg
     1621 ggacgaggaa tgtctacgat gttcagcaga gtatgccaca tgcttgcgct gctcctgcct
     1681 caggccggtg aggacaaggc ggggagccaa cccgggcgcc agtcaggcgc ttcggtgata
     1741 catcaaggag gggactatga tgcgggttcg gagagtcgtg cggaacattg gaatgtgggg
     1801 tgcggggctt gcgatcgccg cggtgttcgg tgttgcttgt gtgcagccgg catcggctga
     1861 ggaaggcacc ggcaacatgg tgttcttcaa aggcgggttc atgaatttga accaggaccg
     1921 gagcgggcag atttttacgg atacggccgg gatcgcgggt gcgaacggag gcaatgccgg
     1981 gtggtatgcc ggcgcggggt tggatctggt gatgtcgaag gatgtctggg gcggaatgga
     2041 caagacgtgg gtggtcggtg aaatcggcct gcaattcaac agcatcaatt caaaccgtgt
     2101 gaacaatgcc gccggaagta ttgccacccg gacgttgacc gacaccctgg ccaccgaccc
     2161 gcagaaggtg cagctcacga tggtgacgat cgacgtggca ccgaagatca aattcatgga
     2221 gggcagcgca ttcaggccct gggtcattcc gatcggtctg gacatccatg tgatcagccc
     2281 tccgtcgaat cggacacagt atctcgatct cggggtgcag ttcggcggtg gattcgaata
     2341 tgcggtgtgg aaggccttta aggtcggggt cgacgcccgg tatcacctga cggcgcgact
     2401 gaccaacacc aacaacgatt acttccaggt cgggccgtat atcgggattt cgttctagga
     2461 ggaaataaag gcggtcagga agacgccctg tttcacaatc gcgacttcgg ctgtaggcga
     2521 aaaagtacga cgctgccatg ccggtcggta ccgcacggtc cgaccggcgt ggcggccttc
     2581 ctcaacgctc gactcatgag ctcgggtctt ggtgatgatc gccgtatatc ttctggacga
     2641 cggttccttg ccagcctcga aaagaacaca ctgcctcgct cccatgtcgg taccttcatg
     2701 agccgaatct gcgtcgtcct cctcacctct aagacgccct cccttgccgc tccacgctct
     2761 cctcggtcat agtgcgctta gtgatgcgag agaaaggaag gtcttgtgtt tgcactggtc
     2821 tggattcact tgctggcggc catggtatgg atcgggggga tggtattcct atccgtcgtg
     2881 ctggtgccgg tgctgaagcg ggatggggca tttgctcagt atgccctgct gttccgaacg
     2941 attgcctatc ggttcagagc tgtggtctgg ggggcgatgg ggatcctcgt ggccaccgga
     3001 ctcacgctgg ctgcaggtcg atcgattccg ttgatggaac catcccgctg gcccacgatt
     3061 tttgccgcca agatcggatt ggtaacgctg ctattcaccc tgacgctgct gcacgatctc
     3121 gtggtcgggc cgcgtgtgcg acggagcttg ggaatccctg aggcagagcg atccgcgaga
     3181 gatcgggtgc tcctgcgcta ctcggtgctc gtcccgcgta tttcgttact ggtcgcgctg
     3241 ctggtgctgt tgctcgccgt cgtcctggcc cgcacctaac ccgcattatt catgagcgtt
     3301 tctgctgcaa tcgcggattc gcagctgcga ctagcctgtc ggcgggcggg ctcg
//
LOCUS       DAOQRM010000228         3327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_32322_length_3327_cov_19.278117, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000228 DAOQRM010000000
VERSION     DAOQRM010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32322_length_3327_cov_19.278117"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKK81_12670"
     CDS             <1..690
                     /locus_tag="PKK81_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNV33786.1"
                     /translation="GEHPHRGFETVTIMYHGKVAHRDSTGSGGVIGPGDVQWMTAASG
                     VVHEELHEKEFAKQGGLLEGIQLWVNLPKAFKMTAPRYQTLVDAEIPAVELDGGVGRV
                     RVIAGEFRAVKGPAKTFSPVHLYDLRLKAGHRTELNLPEGYNSSLFVLHGEVVMNGSQ
                     TVQEVEIALFGQRGERVVVEAKQDATILVLSGEPIAEPIARYGPFVMNTRDEIIQAVH
                     DYQAGKMGHLS"
     gene            687..1328
                     /locus_tag="PKK81_12675"
     CDS             687..1328
                     /locus_tag="PKK81_12675"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNV33787.1"
                     /translation="MTGQALTVDVYSDVVCPWCYIGKRRLEQALESVQVQATARVFWR
                     PFQLNPTMPKAGMDRRVYLEAKFGGPGEMKAIQDRVAAVGTSVGIEFVFDRIARTPNT
                     FDAHRLIWFAQQQGRQDEVVEELFHGYFTEGLNIGQPEILVSLAVRAGLDGEAVVRLL
                     HAQEGVDAVRQEEAHGHQLGIRGVPYFVLNGKAALSGAQPVETFVSAIKQVTG"
     gene            1341..1778
                     /locus_tag="PKK81_12680"
     CDS             1341..1778
                     /locus_tag="PKK81_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV33788.1"
                     /translation="MPVQVYGCNHIVIEVTDAKKAVKFYSDVFGLKMLRGGEGAAWCK
                     LGEHQFMAIFEVEQLQPDRVKHFGLMVRDAEQIKEVRRKLTRKYKLRMEPHFRCDFRD
                     PWGNRIQVGDLSDESLVWLLPYQEVQKAGITFSKPSRVRQGRE"
     gene            1783..2241
                     /locus_tag="PKK81_12685"
     CDS             1783..2241
                     /locus_tag="PKK81_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNV33789.1"
                     /translation="MPDLEKVRTLAKDLTKTYPRSPREMLGGYVIGARCADKCRAFLL
                     GINGDYNYWPCSLAGLLFSFTGITPEQFKEVVATGANDEELAAWLKAQSKVQDQQAII
                     QWNNKMRDLRLSEMAPQVQAYMEDYIPKYVPSHRPVYVYFDVYDLEEKRL"
     gene            2279..>3327
                     /locus_tag="PKK81_12690"
     CDS             2279..>3327
                     /locus_tag="PKK81_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNV33790.1"
                     /translation="MPDATWTEIGSVDELKQRPLQQIVCGQTTLALSYKDGRFAAISG
                     VCNHIGGPLGEGRLDGDYVVCPWHYWKFHHRTGRGEPGYEGDQVATYAVKVEDGRVFA
                     DLTPVTKRQKLPKPSHPLARPIVRADGPIRVLGIATTAMTADQPRFSASDALLEEALA
                     YAREHLQLDTQLIKLRDLSFRACEGFYSKSAEACTWPCSITQMDPTDQMDRVYEAVVH
                     WADVILVSTPIRWGGASSLYYKMVERMNCIQNQETIANRHLLKNKVAAFIIMGGQDNV
                     QGVAGQLMTFFAEVGCQFPQFPFIAHSRGWSAEDMERNNSEVQNSRELREGAQSLVAR
                     AAEMARLMVEGQLAM"
BASE COUNT          694 a          885 c         1104 g          644 t
ORIGIN      
        1 ggcgagcatc cccatcgagg ctttgagacg gtcaccatca tgtatcacgg caaggtggcg
       61 catcgtgatt cgacaggcag cggcggcgtt atcggccccg gtgacgtgca gtggatgacg
      121 gcggcctcgg gtgtcgtgca cgaagagttg catgagaagg aattcgcgaa gcagggcggc
      181 ctgttggagg ggattcagct gtgggtcaat ctgccgaagg cgttcaagat gaccgcgccg
      241 cgatatcaga cgctggtcga tgcggagatt cctgcggtcg agctcgatgg aggagtcggc
      301 cgggtacgtg tcatcgccgg agagtttcgc gcggtgaagg gtccggcaaa gaccttcagt
      361 ccggtccacc tctacgacct gcggttgaag gcgggccacc ggactgaact caacttgccc
      421 gagggatata attcgtccct gttcgtgctg cacggagagg tcgtaatgaa cgggtcgcag
      481 acggtacagg aagtggaaat cgcgctgttc ggtcagcgag gagagcgggt cgttgtggag
      541 gcgaagcagg atgcaaccat cctcgtgctg agcggcgaac cgattgccga gccgatcgcc
      601 cggtacggtc cgttcgtgat gaacacgcgc gacgaaatta ttcaggccgt gcatgactac
      661 caggcgggaa agatggggca tttgtcatga ccggtcaggc gctcacggtc gatgtgtatt
      721 cggacgtggt gtgcccctgg tgttacatcg gcaaacggcg gctggaacag gcgctggaat
      781 cggtccaggt gcaggcgacg gcgcgcgtat tctggcgccc gtttcagctc aatccgacca
      841 tgcccaaggc cggcatggat cgccgggtgt atctcgaagc gaagttcggc gggcccggcg
      901 aaatgaaggc gatccaggat cgcgtggcag cagtgggaac gagtgtgggc atcgagttcg
      961 tgttcgaccg gattgcgcga acaccgaaca cgttcgatgc ccatcgactg atctggttcg
     1021 ctcagcaaca gggccgtcag gatgaggtgg ttgaagaact gttccacggg tacttcacgg
     1081 agggactgaa tatcgggcaa cctgagatac tggtctccct cgccgtccgg gccggattgg
     1141 atggagaagc agtcgtccgt ctgctgcacg ctcaggaggg cgtcgatgcc gtgcggcagg
     1201 aggaggccca cggccatcag ctgggcatca gaggggttcc ctatttcgtg ctgaatggaa
     1261 aggctgcgct ctccggtgcc cagccggtgg agacgttcgt ctctgcgatc aaacaggtta
     1321 cggggtaagg aggtcctgcg atgccggttc aagtgtatgg atgcaaccac atcgtcatag
     1381 aagtcaccga cgccaagaag gccgtgaagt tttattcaga cgtgttcgga ctcaagatgt
     1441 tgcgcggtgg agaaggcgcg gcctggtgca aactgggcga acatcaattc atggcgattt
     1501 ttgaagtcga gcaactgcag ccggatcgcg tgaagcattt cgggttgatg gtgcgtgatg
     1561 cggagcagat caaagaagtc cggcgcaagc tcaccaggaa gtacaagctg aggatggagc
     1621 cgcatttccg ctgcgatttc cgtgacccct gggggaaccg cattcaggtc ggtgatctat
     1681 cggacgagtc cctggtctgg ctcctcccct atcaggaggt ccagaaggcg ggaatcacat
     1741 tctccaagcc ctcgcgtgtt cgacaaggaa gggagtgaca gaatgcctga tctagagaag
     1801 gtccgaacgc ttgcgaaaga tctcaccaag acctatcctc gcagcccgcg ggaaatgctg
     1861 ggaggttatg tcatcggcgc gcgttgcgcg gataaatgcc gggcgtttct cctgggcatc
     1921 aacggggact ataactattg gccctgctcg ttggccggcc tgttgttctc gttcacgggc
     1981 attacgccgg agcaattcaa ggaggtcgta gccaccgggg cgaacgatga ggaactggcc
     2041 gcctggctca aggctcagag caaggtacag gaccagcagg caatcattca gtggaacaac
     2101 aagatgcggg acctgcgtct ttccgagatg gccccgcagg tgcaagccta tatggaagac
     2161 tacatcccga aatatgtgcc gagtcaccgc cccgtctacg tgtatttcga tgtgtacgat
     2221 ctggaagaga agcgcttgtg acaaacggat gtgtggctga cgcctgaagg aactcgacat
     2281 gccggatgcg acatggactg agatcggttc ggtcgatgaa ctcaagcagc gccccctcca
     2341 gcagatcgtc tgcggccaga caacgctcgc attgagttat aaggacggcc ggttcgcggc
     2401 gatttcaggg gtctgcaatc atatcggcgg gccgctgggt gagggacggc tcgacggcga
     2461 ctacgtcgtc tgtccctggc attactggaa gtttcaccat cgcaccggcc ggggcgagcc
     2521 tggatatgaa ggtgatcagg ttgcgaccta tgccgtgaag gtagaggatg ggcgcgtctt
     2581 tgccgatctg actccggtca cgaagagaca gaagctcccg aaaccatcgc acccgctggc
     2641 gcgcccgatc gttcgagccg acggtcccat tcgtgtactg ggcatcgcca cgacggcgat
     2701 gacggcggac cagccgcgct tcagcgcctc cgatgccctg ctggaggagg cactcgcgta
     2761 tgcgcgcgaa cacctgcagc tcgacacgca actgatcaag ctacgggacc tctcgttccg
     2821 ggcctgtgaa gggttttatt ccaagtcggc ggaagcctgc acctggccct gttcgattac
     2881 gcagatggat ccgaccgatc aaatggaccg ggtgtacgaa gccgtggtgc attgggcgga
     2941 tgtgattctg gtatccaccc cgatccgatg gggcggagcc agcagtctct attacaaaat
     3001 ggtcgaacgg atgaactgca ttcagaatca ggagacgatc gccaaccggc acctgctcaa
     3061 aaataaagtc gccgcgttca tcatcatggg tggacaggac aatgtccagg gtgtcgccgg
     3121 tcaactgatg accttctttg cggaagtcgg ttgccagttc ccgcagtttc cgtttatcgc
     3181 ccattcgcgc ggatggagcg cagaggacat ggagcggaac aacagcgagg tgcagaacag
     3241 ccgggagttg cgtgaggggg ctcagtcgct cgtcgcccgc gcggcggaga tggcgcggct
     3301 catggtggag gggcagctgg cgatgca
//
LOCUS       DAOQRM010000229         3316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_32467_length_3316_cov_14.833793, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000229 DAOQRM010000000
VERSION     DAOQRM010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32467_length_3316_cov_14.833793"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKK81_12695"
     CDS             <1..239
                     /locus_tag="PKK81_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33791.1"
                     /translation="FLLCSDGLTAGVSADTILRVVQESSDPQTVSDRLIALSNAAGGT
                     DNTTVIVAMLRHRKPGMWHRMCTQLFAAPTTESS"
     gene            249..980
                     /locus_tag="PKK81_12700"
     CDS             249..980
                     /locus_tag="PKK81_12700"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNV33792.1"
                     /translation="MSQHHPASPTLLVKAPQGGTRELEVSRTPFTIGRKHDNDLCLED
                     LAVSAHHARIVQVQQVLFLEDLTSTNGTFVNEQKIDRRQLRDADSIRLGTHRLIFREA
                     HPITAETAGTGEVLITDDTMVVSRPMGGERPPTEQKIGVVEILSGKSGQSQYCLTKHV
                     SLIGAQDDAVITLTGWFAPKTAAVISRRGEGYVVSQTESGKRILVNGRHIQGEHALRN
                     GDVVEVAGLTMRFLLRDPKGSLVAG"
     assembly_gap    1295..1394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1661..2674)
                     /locus_tag="PKK81_12705"
     CDS             complement(1661..2674)
                     /locus_tag="PKK81_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33793.1"
                     /translation="MLSWSRPDPLTRDLIHLINARRHDHGDTDAAIDTLQAFIEQGRE
                     QDLLTVLAALDEEMAEWMFDLVAEASCAVIMDGPDDDKGAYALMAALELPLQANLGQP
                     LGLKIDPVATADLLHRHLQIPAGVVVRVEPRLLTDATLEGLTLQTLNEHLVSIADSQR
                     TQAIASRLYPPVENTAFLFFQLIGMPDPGLPDSLEEPERRALLEGLVAQCAELALAPD
                     TLEVPVYASYALFDAQNAVRLHRLADETAAVWRDLDPMVRARTIAHLHTQCGNGVYMP
                     VWTDLFDPELPEDHGPVWTSPDVWVFTADLPIEWPGEMLTNRLLAEGCTRFENHIVEA
                     PEN"
     gene            complement(2837..3250)
                     /locus_tag="PKK81_12710"
     CDS             complement(2837..3250)
                     /locus_tag="PKK81_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNV33794.1"
                     /translation="MKSYREELWFETKTRRAYVNITPQVDAVVQKSGVREGMVLVNAM
                     HITASVYINDDEAGLLRDYDEFLERLAPHEATYRHNDTGEDNGDAHIKRQLMGREVVV
                     AITDGKLDFGPWEQIFYGEFDGRRRKRVLVKVIGE"
BASE COUNT          649 a         1037 c          942 g          588 t
ORIGIN      
        1 tgttcctcct gtgctccgac ggcctgaccg ccggcgtgtc ggccgacacg atcctccgcg
       61 tcgtgcagga gtcttccgat ccgcagaccg tctccgaccg tctcatcgcc ctgtcgaatg
      121 ccgccggggg caccgacaat acgaccgtca tcgtggcgat gctcaggcac cgcaagccgg
      181 gcatgtggca tcgcatgtgc acacagttgt tcgccgcacc gaccaccgaa tcatcctaag
      241 gagcgaccat gtcccagcac cacccagcat cacccacgct cctcgtgaag gcgccgcagg
      301 gcggcacacg agaactcgag gtctcgcgca cgcccttcac gatcgggcgg aagcacgaca
      361 acgatctctg cctggaagac ctcgctgtct ccgcccacca tgcgcggatc gtccaggtgc
      421 agcaagtcct gtttctcgaa gatctgacca gcaccaacgg cacattcgtg aacgagcaga
      481 aaatcgaccg gcgacaactg cgcgacgcag acagcattcg tctcggcacg caccggttga
      541 tttttcggga ggcacacccg atcacagcgg aaacggccgg cactggcgag gtcctgatca
      601 ccgacgacac gatggtggtg tcgcgtccga tgggcggcga gcgcccgccg acggagcaga
      661 aaatcggcgt ggtggaaatt ctctcgggaa agagcgggca atcacagtat tgcctgacga
      721 aacacgtgtc gctgatcggc gcacaggacg acgccgtcat cacattgacc gggtggtttg
      781 cgccgaaaac ggccgcagtg atcagtcggc gcggcgaggg atacgtggtg agccagaccg
      841 agagcgggaa acgtatcctc gtgaacggcc gtcacattca gggggaacac gcgcttcgca
      901 acggagatgt ggtggaagtg gccggcctca ccatgcgatt tttgctgcgc gacccgaagg
      961 ggagtctcgt cgcgggttag cccgcattat tcacgagagt ccgtcacgaa accacggaga
     1021 cgccttgcga gacgggcgcg tggagcctct cggggcgagc ccgcccgctt gggtgcgagc
     1081 atctcgcagc caagcttgtc acagcggcgt gtcggtggtt gcagtaggag tcatcgtgaa
     1141 taatgcgggc tagttgagca agcgaccccg tttcggcagg agttgaaaac actggtccga
     1201 gacttcctgg gcatgagatt ccagacactg caccaacgcc ccgccgccta gcggcacctc
     1261 ccgacagaac tgccttcggt ccgcctcaca ggctnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnggcggg aaggaaaccg gtgtcggttc gggggccagg tcgctctgcg
     1441 gcagcgtata gagcaccagg ccccagaccg cgcccaacac cacacaactg agtactgtca
     1501 cgatcgcgat ggaaccgagt cgggatgggc cagccaagga gctcctacgg ggtatgagat
     1561 cgatcctgcc tgatcagcag gatctgggaa gaggcttccc attgcccgtt cgacgcgggc
     1621 gacgatgcgg cacacatgga attgtccggc aaccgccgct tcagttctca ggcgcttcga
     1681 cgatgtggtt ctcgaaacgg gtgcaacctt ccgccagcaa tcgattggtc agcatttccc
     1741 ccggccattc gatcgggagg tcggccgtga acacccatac gtcgggcgac gtccacacag
     1801 gaccgtggtc ctccggcaac tccggatcga agagatcggt ccagaccggc atatacacgc
     1861 cgttgccaca ttgcgtgtgc agatgggcga tcgtgcgcgc ccgcaccatg ggatccaaat
     1921 cccgccagac tgcggcggtt tcgtcggcca accggtgcag tcgcaccgca ttctgcgcgt
     1981 cgaacagcgc ataagaggcg tagaccggca cctccagggt gtccggggcc agcgccagct
     2041 cggcgcattg tgcgacgaga ccttccagca gcgcccggcg ctccggttcc tccagggaat
     2101 cgggcaagcc cggatcgggc atgccgatca attgaaagaa cagaaacgcg gtgttttcga
     2161 ccgggggata gagacgcgac gcaatcgcct gagtgcgctg agaatccgcg atgctcacga
     2221 gatgttcgtt caacgtctgg agggttaacc cttcgagggt cgcgtcggtc aggagacggg
     2281 gttccacccg cacgaccacg ccggccggaa tctgcaaatg tcgatgcagc agatccgccg
     2341 tcgcgacggg gtcgatcttc aatccaagcg gctgccccaa gttggcttgg agcggcaact
     2401 ccaacgcggc catcaaggca taggcgccct tgtcgtcatc cggcccatcc atgatgacgg
     2461 cgcacgaggc ctccgccacc agatcaaaca tccactccgc catctcctca tcgagcgccg
     2521 ccaagacggt cagcagatcc tgctcacgtc cctgctcgat aaaggcttgc agggtatcga
     2581 ttgcggcatc cgtgtcccca tggtcatggc gacgggcgtt gatcaaatga atcaaatccc
     2641 tcgtcagcgg atccggtcgt gaccaactca acatggctgg cctcccatgt cgatacgaag
     2701 tcctgctgaa tggtatctgt tcatgctgtt atccatgttg ccaaactttc cagagcgtgg
     2761 caagcgcgaa tgcggttctt cgtcgacctc agcagcacac catccgtggc agcctcgcca
     2821 cagaacgttc gacgcatcat tctccgatga ccttgaccag cacacgctta cgccgccgcc
     2881 catcaaactc tccgtaaaat atttgttccc acggcccgaa gtccagcttc ccatccgtaa
     2941 tggcgaccac cacctcccgc cccatgagct gccgcttgat gtgcgcatcg ccgttgtctt
     3001 caccggtgtc gttgtggcgg tacgtcgcct cgtgcggcgc gagacgctca agaaactcat
     3061 catagtcgcg cagcagccct gcctcgtcat cgttgatata cacgctggcg gtaatgtgca
     3121 tggcattcac cagcaccatc ccctcccgca ccccgctttt ttgcaccacg gcatcgacct
     3181 gcggcgtgat attcacatac gcgcgcctgg ttttcgtttc aaaccagagt tcttctcgat
     3241 aggacttcat cggtcttcac attcagggtg gtgcgcgatt ctgcaacgag gatgttggaa
     3301 cttgcaagcc cttgcg
//
LOCUS       DAOQRM010000230         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_32630_length_3306_cov_18.111658, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000230 DAOQRM010000000
VERSION     DAOQRM010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32630_length_3306_cov_18.111658"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1061
                     /locus_tag="PKK81_12715"
     CDS             243..1061
                     /locus_tag="PKK81_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33795.1"
                     /translation="MTHSIKTVDDLRWFLARTAGFRGGHITDVHLSKRRIFDEASGRE
                     VMAGSMATVVVRYHTQGILRVAKLTMLGVTDLSLFEQDGSDCSSLGAIQVEMSEGKLR
                     FWFDPDGNLYVVCEEATLEEVSLPASESDMGGEIAQWTFQADSGEAPTVEWLLARLDQ
                     AGVPCIWKAATRRQGRHSLMCWEGELAMAADMAAGAAAALRVEAYGPLDGAGFGLRLQ
                     TRDAAGRLGGRLLSLVTDLVTQRYRGTCLVGQTFLSHEEWVKWNSFRPTHQASG"
     gene            complement(1083..1775)
                     /locus_tag="PKK81_12720"
     CDS             complement(1083..1775)
                     /locus_tag="PKK81_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV33796.1"
                     /translation="MDIAKVERVYTSYSGVYDHIFGKIFHESREACVRNLRIRPEEKI
                     LEVGVGTGIALEYYPKNCEIIGIDLSSGMLAKARQRQSHYHLDHVRLMLMDAGKMDFA
                     DDSFDTVMAAYVVTAVPDYRKVVNEMIRVCKPGGRIIMLNHFSNGNKLIAAVEKVISP
                     LCKHIGFRTDLSLNTVLEGTNLHVARKEKVNPMKFWHLVECVNQKGAKNGSVNGHGSV
                     NGNGNGRHGHHS"
     gene            2030..2650
                     /locus_tag="PKK81_12725"
     CDS             2030..2650
                     /locus_tag="PKK81_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33797.1"
                     /translation="MILAGAFAIVLFVGLIVGDWMYFARLSSDASRYGYGIGRVQDHF
                     AALTVPQLLRTFDQNGFLSLPHGVARVFPEANRMIIRPTHRLFSLRFRTAWPLKGSIE
                     WAPESEGLKMVCTKRAPWSSSILTFLWFSLVGFGTLGFVITYLLQGGLDSLGSVLLGI
                     GVTGVGMIVVAFGLLTITVAYRIENTRLMQVYTELRDVALKSEGAA"
     gene            complement(2785..3141)
                     /locus_tag="PKK81_12730"
     CDS             complement(2785..3141)
                     /locus_tag="PKK81_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33798.1"
                     /translation="MGTQKDHRQGTGSVESLRDEFREHLHIFYSTLKLAAPYDTVEKA
                     LGALTARLKTLSPEELQQISTDSSLRWTCFRQAFVESGLSAKHRGIIAGLARSGAALD
                     LPPEFEPLLNLYRPTS"
BASE COUNT          594 a          876 c         1045 g          791 t
ORIGIN      
        1 aattgttgga tcgtctcgat ctgctgcgcc tcgacaatca gcgtctccag gaacgtgtgg
       61 atgagttgga gcgtcagtta gccgagttca agcagcaaca ggctcttttc tagcgatcgc
      121 tcgtcgtcgt ctccctcctt gtgttctctg cttcacgcgc tcatcgacgg tcccggcatg
      181 ataaggtgga aagagtgcgg cgctcggata acgccacctt ggggaaaggc cgtgggatat
      241 tcatgaccca ttcgatcaag acggtggatg atctgcgttg gtttctggcg cgcacggcgg
      301 ggtttcgcgg cgggcacatt accgatgtgc atctgtccaa gcgccggatc ttcgacgaag
      361 cctcaggtcg cgaggtgatg gctgggagta tggcgacggt ggtcgtgcgg tatcacacgc
      421 agggcattct acgggtcgcc aaacttacca tgctgggcgt caccgatctt tccctctttg
      481 agcaggacgg cagcgactgc tcttctctgg gggcgattca ggtggagatg agcgagggga
      541 aattacgctt ctggttcgat cccgacggga atctttatgt agtctgcgaa gaagcgacgc
      601 tggaagaagt ctcgttgccg gccagtgaat cggacatggg aggcgagatt gcgcaatgga
      661 cgtttcaggc cgattccggt gaggccccca cagtggaatg gctgcttgcc cggctggatc
      721 aggcgggtgt gccctgtatc tggaaggcag cgacgcgtcg gcaaggtcgg cacagtctga
      781 tgtgttggga gggagagtta gcgatggctg ccgacatggc ggccggtgct gcagcggcgc
      841 tccgcgtgga ggcgtatggg cccctagatg gagccggatt cggactccgc ctgcagacac
      901 gtgacgctgc cggtcgcttg ggtggtcgac ttctgagcct ggtgaccgac ctggtcacgc
      961 aacggtatcg cgggacgtgc cttgtagggc agacgtttct ctcacacgag gaatgggtga
     1021 agtggaactc attcagaccc acacatcagg ccagcggatg aggtggagac taaatcagcc
     1081 tgttagctgt gatgtccgtg ccttccgttt ccgttcccat tcacgctccc gtgtccattc
     1141 acgctgccgt tcttggcacc cttctggttg acacattcca ccaagtgcca aaacttcatc
     1201 gggttgactt tttctttacg ggcgacgtgg aggttggtcc cttcgagcac ggtgttcaac
     1261 gagaggtcgg ttcggaagcc gatatgcttg cagaggggag aaatcacttt ttccacggca
     1321 gcaatcagtt tgttgccgtt gctgaagtgg ttcagcatga tgatgcgtcc gcctggcttg
     1381 cagacgcgaa tcatttcgtt cacgactttg cggtaatccg gtacggcagt cacgacatag
     1441 gcggccatca cggtatcgaa gctgtcgtcg gcgaagtcca tttttccggc gtccatcaac
     1501 atcagtcgca catggtcaag atgatagtgc gactggcgct gtctggcctt cgccagcatc
     1561 cctgaggaga gatcgatgcc gatgatttcg caattcttcg ggtagtactc cagggcgatt
     1621 ccggttccca cgccgacttc caaaattttc tcttctggtc ggattctaag attgcgcacg
     1681 cacgcttcgc gcgattcgtg gaagattttt ccgaaaatat gatcgtagac gccggaataa
     1741 ctggtgtaca ctcgctcgac tttggcgata tccatcgtcc ctccgcaaaa tcacgcctct
     1801 ccgcccgcac actctgcagt gcagaagcga cggcgatgag aagtgtggcg tggaaaaatc
     1861 tatgaaggcg ggactgtaat ccggatgact cgtcctcacc cgccggacaa gaacgggctt
     1921 aatgtagcca aagcactcat agggagtcaa ctggttcctc gggtttttga gtgctcctgc
     1981 atctcgtccg ggcttgattc tccgtaaagc cctgtgggaa gtttccgcta tgattttggc
     2041 cggtgcgttt gccatcgtgt tgtttgtcgg gttgatcgtc ggtgactgga tgtactttgc
     2101 ccgtctctcg tccgatgcca gccggtacgg gtatgggatc gggcgggtgc aggatcactt
     2161 tgctgcgctg accgttcctc aactgctgcg cacgttcgac cagaacggtt ttctgagtct
     2221 gccccacggc gtcgcgcggg tctttccgga ggcaaaccgg atgatcatca ggccgaccca
     2281 tcgtcttttc tccctgcggt tccgcacggc ctggccgttg aagggctcca tcgaatgggc
     2341 gccggaatcg gagggactga agatggtgtg cacgaagcgg gcgccctggt cgtcttcgat
     2401 tctcacgttt ctgtggttca gccttgtggg gttcgggacg ctcgggttcg tgattaccta
     2461 tttgctccaa ggcgggctcg attcactggg gagcgtactg ttgggcatcg gcgtcaccgg
     2521 tgtgggaatg atcgtggtcg ccttcggcct gttgacgatt acggtggcat atcgcatcga
     2581 aaatacgaga ctgatgcagg tctatacgga gctgcgtgac gtggcgttga agagtgaagg
     2641 ggcggcctag cccgcattat tcatgacggt tcgtcgcgag attgcggagt cgcgatgcga
     2701 gacgagcggc gtgcccgccc gccgataggc ctgtcgcagc tgcgaatccg cgattgcagc
     2761 agaagcgctc atgaataatg cgggctagct ggtcggacga tagaggttga gcaggggctc
     2821 gaattcaggc ggcaggtcca gtgccgctcc ggatcgtgcc aggccggcga tgatcccgcg
     2881 atgtttcgcg ctcaggccgg attcgacgaa ggcctgccgg aagcaggtcc atcgcaagga
     2941 cgagtccgtg gatatctgtt ggagttcttc tggcgacaac gttttgagtc tcgcggtcag
     3001 ggcccccagg gctttctcga ccgtgtcgta gggcgcggcc agcttgagcg tgctgtagaa
     3061 tatgtggaga tgctcccgga attcgtcccg gagcgattcc accgaacctg ttccctgccg
     3121 gtggtccttc tgtgtgccca tgtcggtctc ctgtgcggtg ccctgttcaa aggaagctta
     3181 cgggaggaac gacgtgcggc gcaacgcgtt ttcgtgcgag gcattcattt ttcttatgta
     3241 tggaggctgt aatgcgttat cgatccttgg ttgtcctcat cggcgtgctt gtgaccggat
     3301 tgaccg
//
LOCUS       DAOQRM010000231         3269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_33201_length_3269_cov_16.589297, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000231 DAOQRM010000000
VERSION     DAOQRM010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33201_length_3269_cov_16.589297"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /gene="thiI"
                     /locus_tag="PKK81_12735"
     CDS             <1..1179
                     /gene="thiI"
                     /locus_tag="PKK81_12735"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNV33799.1"
                     /translation="MRCAIVHYHELALKGRNRDFFEQRLVRNLRLALKDLKIRRIESL
                     QGRIRVTIPDEVTPDLVTERLRRTCGVSNFLLTESVPLDLTAPDLTTIKQVAERQLSE
                     QTFNSFRVTAKRADKRLPLTSMDVEREVGGYLHEATGKAVKLKQPDLTLYIELLTKEA
                     HVAARKIQGPGGMPVGTSGKVACLISGGIDSPVAAYRMMKRGCKAVFVHFSGRPLVSR
                     DSEEKVQELVQLLTAYQYHSQLYIVPFGEIQREIVANAPAAFRVVLYRRIMVRIAEEL
                     ARRHGCWALVTGDSLGQVASQTPENLSVVEAAAELPLLRPLIGMDKIEITEQAQQIGS
                     FTTSIEPDQDCCKLFVPLHPSTRTKPDDILRIERGLEISTFVKQGVEKAELSTFTFPS
                     "
     gene            complement(1163..1747)
                     /locus_tag="PKK81_12740"
     CDS             complement(1163..1747)
                     /locus_tag="PKK81_12740"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNV33800.1"
                     /translation="MVRRVWLVSGCLLALVTVPVGSATAGEVGSAHLLRALVVQPDPD
                     GGVRATATLLFPGGPAVLQSILSEYRKWPELFETRMRVAQVEEQDGRVLTDIYISHAL
                     LPGEQRLLCESQALPGGGLITHLKGGDFTRYHREWRLQPAGDGTQTRAEFDLLVEVKT
                     LVPDWLVAVAMERELNTHFRLVRERALDRITKER"
     gene            complement(2038..2799)
                     /locus_tag="PKK81_12745"
     CDS             complement(2038..2799)
                     /locus_tag="PKK81_12745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV33801.1"
                     /translation="MAQAKILIVDDEVVVAEDIRRQLRALGYLVVGVVASGDEAVRLA
                     GEHRPDLVLMDIKLKGPMDGIDAARIIQTQYGAPVIYLTAFSDEETLERARETLPLAY
                     LIKPFVRSDLRAALELALFRQRVSRIAEQRGRWLDAVVQSMEDAVVTVDQQGRVTMLN
                     PAAEGLTGWSQPDALGKAVQEVMVVLDPESRRSVLHPAVQMLRKGTSADLGGRPFLLV
                     SRNGHEHRISDSAAMIRDERGDPSGVVLVFRPASA"
BASE COUNT          706 a         1080 c          887 g          596 t
ORIGIN      
        1 atgcgttgcg ccattgtcca ctaccacgaa ctcgccctca aaggccgcaa ccgcgacttc
       61 ttcgagcaac ggctcgtccg caatctcaga ctggcgctga aagatctgaa gatccggcgg
      121 atcgaatcgc tgcaggggcg catccgggtg acgattcccg atgaggtaac gcccgacctg
      181 gtgactgaac gactccgccg cacctgcgga gtttcaaact ttctgctgac cgaatccgtg
      241 ccgttggatc tcacggcgcc ggacctcacg accatcaagc aagtcgccga gcggcaactc
      301 tccgaacaga cattcaactc gttccgggtg acggccaaac gcgcggataa acgcctgcct
      361 ctgacctcca tggacgtcga acgcgaagtc ggcggctatc tccacgaagc cacgggaaaa
      421 gccgtgaagc tgaaacagcc cgacctgact ctctacatcg agctcctcac caaggaagcc
      481 catgtcgcgg caagaaagat ccaaggcccg ggcggcatgc cggtgggtac cagcggaaaa
      541 gtggcctgct tgatttccgg cggcatcgac tcccccgtgg cggcctaccg gatgatgaaa
      601 cgtggctgca aggcagtgtt cgtgcatttc tccggacgcc cgctggtaag tcgtgattcc
      661 gaagagaagg tccaggaact ggtccagctg ctgacggcct atcaatacca ttcgcagctc
      721 tacattgtgc ccttcggcga gattcaacgt gagatcgtcg cgaacgcccc ggcggcgttt
      781 cgtgtcgtct tgtatcgacg gatcatggtg cgcatcgccg aagagctcgc gcggcgtcat
      841 ggctgttggg cgctggtcac cggcgatagc ctcggccagg tcgcatcgca aacacctgag
      901 aatctgtcgg tggtggaagc agccgcggaa ctcccgcttc tgcgcccgtt gattggcatg
      961 gacaagatcg aaattaccga gcaggcgcaa cagatcggga gcttcacaac gtcgatcgag
     1021 ccggatcaag actgctgcaa actcttcgtg ccgttacacc cgagcaccag aaccaaaccc
     1081 gacgatatcc tgcgcatcga acggggcctg gagatcagca cgttcgtcaa gcagggcgtc
     1141 gaaaaggccg agctgtcgac gttcaccttt ccttcgtaat ccgatccagg gctcgctccc
     1201 gaaccagtcg aaagtgggta ttgagctccc gctccatggc gacggcgacc agccaatcgg
     1261 ggacaagcgt cttcacctcg accaacaagt cgaattccgc ccgcgtttgc gttccgtcgc
     1321 cggccggttg cagcctccac tcccgatggt accgtgtgaa atctcccccc ttgaggtgcg
     1381 tgatgagccc cccgcccggc aacgcctgcg attcacagag cagccgttgt tcgccgggta
     1441 acaacgcatg tgaaatatag atgtcggtga ggacccgccc gtcctgctct tcaacctgcg
     1501 ccacccgcat ccgggtttca aatagttcag gccatttgcg gtactcggac aggatcgact
     1561 ggagcaccgc tgggccgccg ggaaacagca gggtggccgt tgcccgaaca ccgccgtcag
     1621 gatcgggctg caccacgagg gctcgtagca gatgggctga acctacctct cctgctgtcg
     1681 cgctcccgac cggcacggtc accagagcga gcaagcaacc ggataccaac caaacccgtc
     1741 tcaccacgaa tcgtagcttg tgcatcacag tataacggtg gggttcgtga gccggacgaa
     1801 attgcgctcc tcagacggac gtcgcacccg gaaaattgca gaaccgacac tcatgcgttc
     1861 ctccacacct tgaaaccgtc tcaatacccg ggcgaccgga accgctcgcc ggccttcagc
     1921 gctactcaag aagtatactc attgaatgcg cccagtgcga gcgcaacgac gggacaaata
     1981 caattatgcc tgctgagcgg atgatcacac ggttgaaaac ggcgggaacc tgaacggtta
     2041 cgcagaggca gggcgaaaca ccagtacgac tcctgacggg tctccccgct catcacggat
     2101 catggccgcg ctgtcgctga tccgatgctc gtgcccgttg cgactcacga gaagaaacgg
     2161 gcgcccgcca agatcggcag acgttccctt acgcaacatc tgtacggccg gatgcagcac
     2221 ggacctccga ctctccggat ccaacaccac cattacttcc tgaactgcct tccccagggc
     2281 atcgggctgc gaccagccgg tcaatccctc agccgccgga ttgagcatgg tcacccgtcc
     2341 ctgctggtcg accgtcacaa cggcatcttc catcgactgg acgaccgcat ccagccaccg
     2401 cccgcgttgc tcggcaatac gcgacacacg ctgccggaag agcgcgagtt ccagcgcggc
     2461 gcgcaaatcg ctcctgacaa acggcttaat caaataggcc aacggcaggg tttccctcgc
     2521 gcgctccagt gtctcctcat ccgaaaaggc cgtcaggtag atcaccggtg cgccatactg
     2581 ggtttggatg atccgggccg cgtcgatccc atccatcggc cccttcagct taatatccat
     2641 gagaactaaa tccggccgat gctccccggc cagccgtacg gcctcatccc cggatgccac
     2701 cacacccacg accagatacc cgagtgcgcg cagctgacgt ctgatgtctt cagccacgac
     2761 cacttcgtca tcgacgatca gaatttttgc ctgcgccata gtacaacccg atgaaacgag
     2821 atcggaaaat tggtatcgcc gctcacacca cacaccgctc gagcgcacta cacacgtatc
     2881 cgcacagcgc tcgatacccc accgcaacgc aactaacatg ccacctggcc ttccctgtac
     2941 tggcatcatg ttatgccggg cgggattctt gcccccatac accgacggtg gtttttgaac
     3001 agtccttgga cagaatcggc agaaaacaag gcttgcgtgg accaggcgct ccgggaaaga
     3061 ttcgctgcgg aaatgaccgc tgtgatacga tgccgctctt taacggagtt gatccaacat
     3121 gtcttggctg attcgtccga tcctccccct actgctcggc tgtgtcctcg catggagcct
     3181 tattcccgac gaggggcagg cccaagcgcc gcctgactcc tccgcgacga ccgaacaatc
     3241 ctgcagtttc ggcatggcga acggcgatc
//
LOCUS       DAOQRM010000232         3236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_33727_length_3236_cov_20.541339, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000232 DAOQRM010000000
VERSION     DAOQRM010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33727_length_3236_cov_20.541339"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1723
                     /gene="pyk"
                     /locus_tag="PKK81_12750"
     CDS             272..1723
                     /gene="pyk"
                     /locus_tag="PKK81_12750"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNV33802.1"
                     /translation="MRKAKIVCTIGPASDGAVVLDQLIRSGMDAARLNFSHGTHDSHG
                     RAIKTIRDTADRHGVAIAIIQDLQGPRIRVGEIDGVLELIAGRRVRLRTMSLRSGGQI
                     GARTVIDAGTVQDIPVTYQALTRDIRPGAKILIDDGLVELTADRIVDGAVECTVVAGG
                     RVTSHKGMNLPGTVVSAPTLTEKDREDLRFGVAQGVDYIALSFVRGAQDIMAAKELIA
                     ECGGDVPVIAKIERQEAVTDLEAILVHADGVMVARGDLGVELGPEAVPVLQKRIIATA
                     NRQRRLVITATQMLESMTQHLRPTRAEASDVANAVFDGTDAVMLSAETAVGHYPVEVV
                     QVMDRIIRAAEVETGPCFVRRSQGEQGQDSIPEAISLSAYSAAATIGASAIVAFSERG
                     TTARLVSKQRPVAPIIGLTPFETVRRQMALYWGVLPHTVPQIPTTDERVNEAERRLKA
                     EGLARSGQRIVILSGTRIGQPGGTNLMKLHEVA"
     gene            1728..2291
                     /locus_tag="PKK81_12755"
     CDS             1728..2291
                     /locus_tag="PKK81_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33803.1"
                     /translation="MNSPRRSVLITFIAAVLLLPASVTPGLAQTNAVSHSPAKVVEKY
                     FALDNKGVRLDAGSFEAVAGLVDWKEEPAWGKVIVINGFSVPDDFRQWEIVNRLEVLV
                     PVEFHVLGVMYLDTAGFVPEPGTEQVRVRVKVQGARWKIMEPILPPHVGQKRMLNVVR
                     QAMLEEKDGVRHESLAALQAELRKARE"
     gene            2288..2938
                     /locus_tag="PKK81_12760"
     CDS             2288..2938
                     /locus_tag="PKK81_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNV33804.1"
                     /translation="MTKKAEIDLLIFDLDGTLIESKWDIADSVNLTLRDLGVPERPQE
                     EIFGFVGDGVKKLLRLAVGEGNKDLYDEALRVFRGHYLEHCLDRTSFYPGIDQVLTHF
                     SAKPKAVATNKAIEYTHVILKGLGPQHFVYVVGGDNGFGLKPEPGMLVHVMEQLGVEK
                     DRTVLVGDSTNDINGGHNAGIRVCAVGYGMGNRQKMAACEPDWFIERPEELMELFI"
     gene            2935..>3236
                     /locus_tag="PKK81_12765"
     CDS             2935..>3236
                     /locus_tag="PKK81_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNV33805.1"
                     /translation="MMSITGARTMGALILAGVLAAAVPGQAGSPSLADRVIEHKLANG
                     MTVLMVERHQAPIVSVNMTFGVGGVNEQVGQTGLAHLYEHMAFKGTRTVGTRDYER"
BASE COUNT          640 a          853 c         1110 g          633 t
ORIGIN      
        1 cagcaagcct tgcatcgtga caaagtccaa ctgatcggtt gactggctgt ccgaagcgct
       61 catgcgtagc ctcgtgtgct ggttcgtaag taaattttaa tgagactgag cgtggttcat
      121 caccgaaaag tatatggatg ttcttttgcg gcatggtgac gggtttgtta gaatgacccg
      181 ttgccgaccg aatcacggat gcatcgagcc tcaccgttca cgtacaagat ggtgtgccgg
      241 gccaatcttg tcaaggcaga agaacagcgc catgcgaaaa gcgaaaatcg tctgcaccat
      301 cggtccggcg agcgatggcg ccgtcgtgct cgaccagctc attcgaagcg gcatggatgc
      361 ggcccgtttg aatttttccc acggcacgca cgactcgcat gggcgggcga tcaagacgat
      421 tcgcgacacg gccgatcgtc acggggtggc gattgcgatc attcaggatc tgcagggacc
      481 gcgcattcgc gtcggcgaaa tcgacggcgt gctggagctc atcgccggtc ggcgagttcg
      541 gctgaggacg atgtccttac gatccggcgg gcagatcggt gccaggacgg tgatcgacgc
      601 gggtacggtg caggatatcc ctgtcaccta tcaggcgctg accagggaca ttcggcccgg
      661 ggccaaaata ctgatcgacg atggactggt ggaattgacg gcggatcgaa tcgtcgatgg
      721 tgcggtagag tgtaccgtgg tggcaggcgg acgtgtgacg tctcataagg ggatgaatct
      781 gcccggcacg gtcgtgagcg ctccgaccct gacggagaaa gaccgggaag atttgcgttt
      841 cggcgtggcg cagggcgtgg actatatcgc gttatccttt gtccgtggag cgcaggacat
      901 catggccgcc aaggaattga ttgccgaatg cggcggcgac gtgccggtga tcgccaagat
      961 cgagcggcag gaggccgtca cggacctgga ggccattctc gttcacgccg acggggtcat
     1021 ggtggcgcgc ggagatttgg gcgttgagct cggcccggaa gccgtgccgg tgctgcagaa
     1081 gcgcatcatc gcgacggcga atcgccaacg acggctggtg atcaccgcga cgcagatgct
     1141 cgagtccatg acacaacatc tgcggccgac cagggcggaa gcgtcggatg tggccaatgc
     1201 cgtgttcgat ggcaccgatg ccgtgatgct ctccgcggaa acggccgtcg gccattaccc
     1261 ggtggaagtc gtgcaggtga tggatcggat catacgggcg gccgaagtcg aaacggggcc
     1321 ctgttttgtc cgtcgctcgc agggcgagca gggacaggat tctattccgg aggccatctc
     1381 gctctcggcc tactcggctg ccgcgacgat cggtgcgagt gcgatcgtcg ccttcagtga
     1441 acggggaacg acggcccggc tggtctccaa gcaacgtccg gttgcgccga ttatcgggtt
     1501 gacgccgttc gagacggtcc gccggcagat ggcgttgtat tggggggtgc ttccccacac
     1561 cgtgccacag attccgacga ccgatgagcg ggtcaacgag gctgagcggc gcttgaaggc
     1621 cgagggtttg gcacggagcg gacaacgcat cgtcattttg tccggcacca gaatcgggca
     1681 accgggcggg acgaatctaa tgaaattgca tgaggtggct tagtcgcatg aacagtccac
     1741 gacgcagcgt gctcatcacg ttcatagcgg cggttcttct gttgccggcc tctgtgactc
     1801 cgggcctggc tcaaaccaat gcggtgtcgc attctccggc gaaggtggtg gagaaatact
     1861 ttgccctcga caacaagggg gtgcggctcg atgccggttc cttcgaggcg gtggcgggcc
     1921 tcgtggattg gaaggaagag ccggcctggg gcaaagtcat cgtcatcaac ggcttttcgg
     1981 tgccggatga ttttcgccag tgggagattg tgaacaggtt ggaggtattg gtgccggtgg
     2041 agtttcatgt gctgggcgtc atgtacttgg atacggccgg gttcgtgccg gagcctggca
     2101 cggagcaggt gcgtgtccgc gtgaaggtgc agggggcacg atggaaaatc atggagccga
     2161 ttctcccgcc ccatgtcggc cagaagcgga tgctgaatgt ggtccggcag gccatgctgg
     2221 aagagaagga cggagtgcgc catgagtcgt tggcggcctt gcaggccgag ttgcgaaagg
     2281 cacgagaatg acgaagaagg ctgaaatcga tttgctgatt ttcgacctcg acggcacgtt
     2341 gatcgagtcg aaatgggata tcgccgacag cgtcaatttg acgttgcgcg atctgggtgt
     2401 gccggagcgc ccgcaggagg aaattttcgg attcgtcggc gacggcgtga agaagctctt
     2461 gcggctggcg gtcggcgaag gcaacaagga cttgtacgat gaagcgctcc gggtctttcg
     2521 cggacattat ctggaacatt gcctggatcg cacctcgttt tatccgggga tcgaccaggt
     2581 cctcacccat ttctccgcca agcccaaggc cgtggcgacc aataaggcga tcgaatatac
     2641 ccacgtgatt ttgaagggcc tcggcccgca acactttgtc tatgtggtcg gcggcgacaa
     2701 cgggttcggt ctcaagcccg agcccggcat gctggtgcat gtgatggaac aactcggagt
     2761 cgagaaggac cgtacggtgc tggtcggcga cagcaccaac gacatcaacg gcggtcacaa
     2821 tgcaggcatc cgggtctgtg cggtggggta tggcatgggc aaccgtcaga agatggccgc
     2881 ctgtgagccc gattggttta ttgaacgacc ggaggaactg atggagcttt tcatatgatg
     2941 agcatcacag gggcgagaac aatgggtgcg cttatcctgg ccggagtgct tgcggcagcc
     3001 gtgccgggtc aggccgggtc cccgagcctg gccgaccggg tcatcgagca caagcttgcc
     3061 aacgggatga ccgtcttgat ggtagaacgg caccaggccc cgatcgtcag cgtcaacatg
     3121 acgttcggag tcggcggggt gaatgagcag gtcggccaga ccggcctggc gcatctgtac
     3181 gaacatatgg cgttcaaggg cacccggacg gtcggcacgc gggattacga gcggga
//
LOCUS       DAOQRM010000233         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_33922_length_3224_cov_15.599558, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000233 DAOQRM010000000
VERSION     DAOQRM010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33922_length_3224_cov_15.599558"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..406)
                     /locus_tag="PKK81_12770"
     CDS             complement(272..406)
                     /locus_tag="PKK81_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33806.1"
                     /translation="MSAHKSFSELSLKNHQYKKLALASAILFVAVIAILVLELIGAKH
                     "
     gene            complement(447..773)
                     /locus_tag="PKK81_12775"
     CDS             complement(447..773)
                     /locus_tag="PKK81_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33807.1"
                     /translation="MKATQTELEHLEMASTTILSQANDRLRVLSHSLQEAGQFERGAL
                     VRALHAEIRHTLPLITLRLNELGRQPERPDAPPHAQQVAESFYILDRALRQLRALASD
                     VRNYNA"
     gene            complement(926..1576)
                     /locus_tag="PKK81_12780"
     CDS             complement(926..1576)
                     /locus_tag="PKK81_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV33808.1"
                     /translation="MNNIRVLLVEDHTLVRAGFRVLLEKIEGILVIGEVSDGRSATKL
                     AKELDPDVVLMDIAMAGMNGLEATRRIRQESPRTKILMLSMYTNEEYLKEALRAGAAG
                     YLLKDADRNELELAIKAVWRGETYLTPSMTKFAVDAYCQQGEAQTGPLARLTGRQREI
                     LQLIAEGCSTKQIAQRLDLSVKTVETHRAQLMERLDIHDVPGLVRLAIRTGLVQSD"
     gene            complement(1569..3104)
                     /locus_tag="PKK81_12785"
     CDS             complement(1569..3104)
                     /locus_tag="PKK81_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV33809.1"
                     /translation="MGEGHRNRRQTLAILSTSPTGIITSFNEASERLLGYSAEEVVGA
                     LPLAALHDPSELQQRAAACTSKPETRATDIFRIIVANAEPGHTTEHDWTYLHRNGRPL
                     PVHLSISAFPDGSGHIAGYILVLQEIREEIRSNDVLQHHAKLLDLANDAILVRDLERD
                     TIDYWNDGAVRLYGWTSKEAAGAYIHDFLKTTFPQPLADIKKTFLAKGYWAGELLHTT
                     RNGQRITVSSRWTLLKTSDGAASASLELNTDITEQKRAQEALKAAHEELEHRVAERTA
                     ALSDANSRLRILSRRLMEIQELERRAIARDLHDEIGQALTAIKMNLRELGEQRDGSTA
                     AADGKPLSDSLQILDQVLRHIRNLALDLRPSMLDELGLVPALRWYIGRQAERAGWKVQ
                     FLADEVMTRPSPEVEISCFRLTQEALTNVARHAKATVVEVRLEMGRRQLDLIIRDNGI
                     GFDSNLIRTGARAGTSVGLSGMEERVRLAGGHMVLQSAPSTGTEIRATFPIGPESAET
                     EHE"
BASE COUNT          612 a          902 c          949 g          761 t
ORIGIN      
        1 ttttcatgct ctcaacggat agatggtcat tgctcaaaag gtgcacggcc tgtatcacca
       61 tttctccgaa gcagaggctg atagtcacaa gtacaagaac gacggtgccg gaggcgagga
      121 tggtggcagg tcgaatggat gacactggtg cttcgtcact gacggatggc ccgcagagag
      181 gggtatctga cggacccttg gcgttctggc gtgcccaatt gcgtttcatc gggaataccc
      241 gtccatgtcg aggcgtgctc gtgcggagcg atcagtgttt ggccccgatt agttcgagga
      301 cgagaatggc aatgacagca acaaagagaa tggcagatgc cagggccagt tttttgtatt
      361 ggtggttctt caggctcagt tcactgaagc ttttatgcgc agacatgagt cgtgttcctc
      421 ggtagcgtct tttgaaagcg agcggatcat gcgttgtagt tacgcacatc cgacgccaag
      481 gcacggagct gccggagcgc ccggtcgagg atgtagaagc tctcggcaac ctgttgtgca
      541 tgcggcggag catccggacg ctcaggctgt ctcccaagtt cgttgaggcg cagtgtgatc
      601 aacggaagag tgtgtcgaat ctcagcgtgt aaagcgcgga caagtgcacc ccgttcaaat
      661 tgcccggcct cttgcaggct gtgagagaga actcgtaagc gatcgttggc ctgagacagt
      721 atcgtcgttg acgccatctc aaggtgctcc agttcggtct gtgtagcctt catcgattcc
      781 gagtccattg cggctctgct atccctgttg gtcacgtgct agccgcgggt agggtgccac
      841 aatggaccat acccgcggaa tcagggatac cctggaaatc gatccaatta cacctgagga
      901 tggggggact tcggcgttgt gggggctagt ccgactggac gagaccggtg cgaatggcta
      961 gccggactag tcccggcaca tcgtggatat cgagccgctc catgagctgc gcgcggtggg
     1021 tttcgacggt tttcacactc agatcaagac gttgtgcgat ctgcttggtc gagcatccct
     1081 ctgcgatcag ttggaggatt tcacgctggc gaccggtcag ccgtgccaga gggcctgtct
     1141 gcgcttcgcc ttgttgacaa taggcatcga cggcaaactt ggtcatgctc ggtgtcaggt
     1201 aggtctcgcc ccgccatacg gctttgatgg ctaattccag ttcattgcga tccgcgtctt
     1261 tgagcaaata accggcggcg cctgcgcgaa gcgcctcttt gagatactcc tcgttcgtat
     1321 acatagagag catcaaaatc ttggtccttg gagactcctg tcgaatgcgg cgggtcgctt
     1381 cgagcccgtt catgcccgcc atggcaatat ccatgagcac gacatcgggg tcgagctctt
     1441 tcgcgagctt cgtggcgctt cgaccgtcgc tgacctctcc gatcacgagg atcccctcga
     1501 ttttttccag caacacccgg aatcctgcgc gcaccagggt atgatcttcg acaaggagta
     1561 ctcgtatgtt attcatgttc tgtctcggcc gactccggac ctatcggaaa tgtcgcgcga
     1621 atttcagttc cggtcgacgg ggctgattgg agcaccatgt gtcctcctgc gaggcgcacc
     1681 cgttcttcca tcccggagag ccccacactc gtccccgcac gagcgcccgt gcggatgagg
     1741 ttcgagtcaa acccgatccc gttgtcgcgg ataatgagat cgagttgccg gcgacccatt
     1801 tcaagacgaa cttccacgac tgttgctttc gcatgccgcg cgacattcgt caaggcctcc
     1861 tgggtgagcc gaaaacagga gatttccact tcgggagagg ggcgcgtcat cacctcatcg
     1921 gccagaaact gaaccttcca tccggcacgt tcggcctgtc ttccgatata ccatctcagc
     1981 gctgggacta ggcccagttc atcaagcatg gagggtctca agtccaacgc caggttgcgt
     2041 atatgccgga gcacttgatc gaggatttgc agactgtcgg aaagcggttt gccgtccgca
     2101 gcggcggtcg aaccatcacg ctgctcgcca agctcacgca ggttcatttt gatggccgtc
     2161 aacgcctgtc cgatctcatc gtgcaaatcc cgggcgatcg cgcgacgttc caactcttgg
     2221 atttccatca gccgtctgga gaggatgcgt aagcggctat tggcgtcgct caacgccgcc
     2281 gtgcgttccg cgacacgatg ctccaattct tcatgtgcgg ctttcagcgc ttcttgcgcc
     2341 cgtttttgct cggtaatgtc cgtattcagc tcgagacttg cgctggctgc gccatctgag
     2401 gttttgagca acgtccaccg gctggagacg gtgatcctct ggccgtttcg agtcgtgtgc
     2461 aggagttccc cggcccaata gcccttcgct aaaaaggtct ttttgatgtc cgccaatggc
     2521 tgcggaaacg tggttttcag gaaatcgtgg atataggcgc ccgcggcttc cttgctggtc
     2581 cagccataga ggcgcaccgc cccgtcattc caatagtcga tcgtatcgcg ctccaagtcg
     2641 cggactagaa tcgcatcgtt ggcgaggtcg agcagttttg cgtgatgttg gagcacgtcg
     2701 ttcgaccgta tctcttcgcg gatttcttga agcaccagga tataccccgc gatatggcct
     2761 gatccgtccg ggaatgcgct gatcgagagg tgaaccggaa ggggccggcc gttgcggtgc
     2821 agataggtcc aatcatgctc tgtcgtatgg ccgggttcgg cgttcgcgac gatgattcga
     2881 aatatgtccg tcgccctggt ctccggtttg gatgtgcacg cggcagcacg ttgctgcaat
     2941 tcgcttggat catgtaacgc cgcgagggga agcgccccca cgacctcttc cgcggaatac
     3001 cctaagaggc gctcagaggc ctcgttgaag ctcgtaatga ttccggtcgg ggaggttgag
     3061 agtatggcaa gcgtctgccg ccggttgcga tgtccctcgc ccatctcgcc tccgttcctt
     3121 ccgcttccat ttcgggagga atatggtggt tgccagtcaa cgggagcctc ttaggctagc
     3181 attagatttc ttccaattgc tatcgctgtc agatgattgt cggc
//
LOCUS       DAOQRM010000234         3221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_33984_length_3221_cov_17.684460, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000234 DAOQRM010000000
VERSION     DAOQRM010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33984_length_3221_cov_17.684460"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKK81_12790"
     CDS             <1..376
                     /locus_tag="PKK81_12790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV33810.1"
                     /translation="QPSGRERREFYRITVLLPICIRHETDDTVGECTEKSVNLSGGGV
                     GVTVTESYTPGEVLSLTLLLPEQVPFTSSIEVLRLDPLPVPGGAYRLHARFVRMTTQN
                     RELLIRYIVRFQRDHLQEHYSV"
     gene            520..1527
                     /locus_tag="PKK81_12795"
     CDS             520..1527
                     /locus_tag="PKK81_12795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV33811.1"
                     /translation="MRGLKWFLGLLVALATIGALYQIVGMVLDRKQHPPIGRLIDIGG
                     HRLHLYCIGQGSPTVVLEAAAPGWSLYWSTVQPEVAQVTRVCAYDRAGLGWSERGPLP
                     RTGRRLARELHQLLSRGGIPGPYILVGHSLGGFVTRLYREEHPLEVVGMVLVDAGHES
                     EMRQAEFRSFANAGKSMLPVIRAMTMLGIPRLMASYDQLPPLLTGQEEKVPTEIRPML
                     RAGWLRTGYFSTLTDESDALIETLEQVRHTGSLGDLPLVVVTATGPLWWPDMPGQVNP
                     AKFRKMWLDLQQNLTTLSSNSRQVFADQSSHFVQFDQPTLISETIRQMVIATRLASRT
                     K"
     gene            complement(1577..>3221)
                     /locus_tag="PKK81_12800"
     CDS             complement(1577..>3221)
                     /locus_tag="PKK81_12800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNV33812.1"
                     /translation="ASHIPLPPDDGHDARPNEFDSVAAGASPAAGSVVERLDSASSVV
                     DGVVAGFLFLFVFGSGLIHIESFPPLWFDEGWTVCVARTWVELGHYGCLLRGEPAPPS
                     LAAHFPVVASVAASFTLFGVGVWQTRLVGLLYTLGAFLLLYALARRLYGRSIAIAALA
                     LLLLVPLKWSIHPLYIGRQVLGEMPLLCFLLAGYVCFLRSTHRPLWQVATIGCWALAW
                     MTKAQVAPFLVASIAGTMVVMSLRGDWSVVGRLAVAMIGSWGGCRLLLYAKDWLLAGH
                     IMPHPPVDGMTEAIALVFVPSIRLETIRYLFVSWPEYPLGLAYAAWRVGGTSGSVAKV
                     SVEQTVQTMLLLLAGSWLAWFAFLSAGEPRYALPGLFLAAPFTAALFHDFTRGFDVAF
                     LTRTLTGLLRSRHVTRQGIKAVVVVMLLVMMGWVAVQERYAFRAREDDRDLFAVTHYL
                     NTATPPTALIETYDSELFLFLHRPYTYAPPRALVEIIRREQGEAKAVTYDPLESRPDY
                     LVVGEYGRWAGFYKPLIAQNRVRLVTSIGRYQIYEPVRR"
BASE COUNT          699 a         1026 c          933 g          563 t
ORIGIN      
        1 acagccgtcc gggagagaac gccgcgaatt ttatcgcatc accgtgcttc tgcccatctg
       61 cattcggcac gagaccgacg acacggtagg tgagtgcact gagaaatcgg tcaacctcag
      121 tggcggcggt gtcggcgtga cggtcactga gagttacaca cccggtgaag tcctttccct
      181 cacactgctg ctccccgagc aagttccctt cacatcttct atcgaggtac tgcggcttga
      241 tccccttccg gtcccaggcg gtgcgtaccg cctccacgcc cgcttcgtca ggatgaccac
      301 ccagaatcgg gagctgctga tcagatacat cgtgcgtttt cagcgcgacc acctgcagga
      361 acactattcc gtctgaagcg cagagaaggc ttggcggctt ggcagccccc gcacacgagg
      421 gcagagatgt tcattgggag tgccgctcgc ccggtcttgg gcgacacttc gactcgtccc
      481 ggtttgcttc ttccatccat gacaggtgat catgtcccca tgcgtggact caaatggttt
      541 ctcggcctgt tggttgcgct ggcgacaatc ggtgcgctgt atcagatcgt cgggatggtg
      601 ctcgatcgga aacagcatcc gcccatcggc cggcttatcg acatcggcgg tcatcgactc
      661 catctctact gcatagggca gggcagtccg acggtcgtgc tggaagcagc cgctcccggc
      721 tggtcgctct actggagcac ggtgcaaccg gaggtggcac aagtcacgcg cgtctgcgcc
      781 tacgatcgcg ctggcctcgg ctggagtgag cgtggcccgt tgccccgcac cggccggcgg
      841 ctggcgcgag agcttcatca gttgctgagc cgcgggggca tccccggccc ctacattctc
      901 gtgggccatt cgctcggcgg cttcgtcacc cggctctacc gcgaagaaca tcccctggag
      961 gtcgtcggta tggtgctggt cgatgcggga catgaatcgg aaatgcgcca ggccgaattt
     1021 cgatctttcg ccaacgccgg caaatcgatg ctccccgtca ttcgcgccat gacgatgcta
     1081 ggcattccac gcctgatggc ctcgtatgac caactgccac cgctgttgac cggacaggaa
     1141 gagaaggtac ccacggagat tcgaccaatg ctacgtgcag gttggctgcg gaccggctat
     1201 ttctcaacgt tgactgatga aagcgatgcg ctgattgaaa ccctggaaca ggtgcgccac
     1261 accggttcac tcggcgatct tccgttggtc gtcgtcaccg caaccggccc cctgtggtgg
     1321 ccggacatgc ccggccaggt gaaccctgcc aagttcagaa aaatgtggct ggatctacag
     1381 cagaatttaa caacactctc ctccaacagt cgccaggtgt ttgccgacca gagcagtcat
     1441 ttcgttcaat tcgatcagcc gacgctcatc agcgagacca ttcgtcagat ggtcattgcc
     1501 acccgcctgg catcacgtac caaatagaca tgaacctatg cacgcgcgcg aggaggaaat
     1561 ttttgcggcg tgatagttac cggcgcacgg gctcgtagat ctggtagcgc ccgatgctgg
     1621 ttacgagtcg cacccggttt tgcgcgatca acggcttgta aaatccggcc catcggccgt
     1681 actccccgac cacgagatag tccggcctgc tttccaacgg atcgtacgtg acggccttcg
     1741 cctctccctg ttcgcgccgg ataatctcaa ccagggcgcg gggcggggca taggtatagg
     1801 gtcgatgcag gaatagaaac agttcgctgt cataggtctc gatcaatgcc gtcggcggcg
     1861 tagccgtatt caggtagtgc gtcaccgcaa acaggtcacg atcgtcctca cgcgcgcgaa
     1921 acgcatagcg ctcctgcacg gcaacccacc ccatcatcac caggagcatg acgacgacga
     1981 ccgccttgat tccctgcctc gtcacatgcc tgcttcgcag gaggccggtc agagtcctcg
     2041 tcaggaacgc cacatcgaat cctctggtga aatcgtgaaa gagcgctgcc gtgaagggcg
     2101 ccgcaagaaa caacccgggc agggcatagc gcggttcacc ggcgctgaga aacgcaaacc
     2161 aggccaacca actccctgcc agcagcagca gcatggtctg caccgtctgt tcgacagaaa
     2221 ccttggcaac ggaaccggaa gttccaccta cacgccaggc cgcataggca agcccgagcg
     2281 gatactccgg ccacgacacg aacaggtatc ggatcgtctc aagacgaatg gacggcacaa
     2341 acaccaacgc aatcgcttct gtcattccgt ctacgggcgg atgcggcatg atgtggcctg
     2401 ccaacagcca atccttggca tacagaagaa gccggcaccc accccatgaa ccgatcatgg
     2461 cgacggccag ccggccgaca accgaccagt ccccgcgaag gctcatgacc accatggtcc
     2521 cggcaatgga ggcgacgaga aacggcgcga cctgcgcctt ggtcatccac gccagcgccc
     2581 agcatccaat cgtagcgacc tgccagagcg gacggtgcgt gctccggaga aagcagacat
     2641 acccggccag gaggaagcac aagagtggca tctcgcccag cacttgtcga ccaatataca
     2701 ggggatggat cgaccacttc agaggcacaa ggagcagcag cgccaaggcg gcaatcgcga
     2761 tagatcgacc gtagaggcga cgggccaacg catagagcag caggaaggcg ccgagggtat
     2821 agagcaatcc caccagtctc gtctgccaga caccgacgcc gaacagtgtg aaacttgccg
     2881 ccaccgaggc caccaccgga aaatgcgccg ccaatgaggg cggcgccggc tcaccgcgta
     2941 gcaggcaccc gtaatgcccc agctcaaccc aggtgcgcgc gacacagact gtccagcctt
     3001 cgtcgaacca cagcggaggg aaactctcga tatgtatcag gccgctgccg aacacgaaca
     3061 agaataagaa tccggcaact actccgtcca caaccgatga agcggagtcg agccgttcga
     3121 ccacggagcc ggccgcaggt gaggcaccgg ccgccacgga gtcgaactcg ttagggcgtg
     3181 catcatgacc gtcgtcaggc ggtaacggaa tgtgagaggc g
//
LOCUS       DAOQRM010000235         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_34340_length_3196_cov_19.072588, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000235 DAOQRM010000000
VERSION     DAOQRM010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34340_length_3196_cov_19.072588"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..1294
                     /locus_tag="PKK81_12805"
     CDS             389..1294
                     /locus_tag="PKK81_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV33813.1"
                     /translation="MSRGIIFGVGLFALTLLAFLCIPRHLPVTSSATGRSAFSAHIEN
                     GQLTLSGTVASEEAKGAAVARAQDLAKSLRLRVTDNLNIIEDGQAAGWEAALPALVTQ
                     AVTLQHHQATLSLSGQTVTVKGAVPSADAKTKLLHEVASLLGTSIHVQDQVTVASSGS
                     ASQANLTPASQPSAAPHASRAQVQAGLDEILRGEHIAFESNSAVLTSKGRAVVDKLVP
                     ALKRAPDAVIEIGGHTDSYGDPDYNLQLSRTRAEAVRQYLVEHQVPNRLTAVGYGSTR
                     PLSQDRTRAASKKNRRIEFRVKEER"
     gene            1299..2549
                     /locus_tag="PKK81_12810"
     CDS             1299..2549
                     /locus_tag="PKK81_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33814.1"
                     /translation="MGAMILQMVGCLLVAATIGLMMGWLLRSFATSEKRQQLSEIATR
                     LRGREHELDTLNHELKVRTSAVQMLEGKMITSEAALKDLTADLGTKVEQLTALQTEVR
                     EKGVRLHAAERERDTLRREVEEAEARIKAQSTGFAEMQAQMENAEDVLIARDQEIATL
                     KTWVEQLAPKDAEIARLRTRTEELEPFLEKSRRLEREQEQDRARASAILQAKEQQLAQ
                     TQARLNAIELDLANLRTQSQEDLARFGETLQERDAEIQRLRTTVEELDMFRGEVEKKD
                     LALREAEERRVMDVSEREEEIGALRKRLVEYRVAQRFSAQAKSTGATESGSTGSATTK
                     RSAGKGHPAQKDDLKQIHGIGPVMERVLNRMGMFTFRQIAEWKEQDVEHMASELNTFP
                     DRIRHDNWIDGAKEQHFLKYGEEL"
     gene            complement(2651..>3196)
                     /locus_tag="PKK81_12815"
     CDS             complement(2651..>3196)
                     /locus_tag="PKK81_12815"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNV33815.1"
                     /translation="PFSRFDALSIALVLMAALAWTVLPLASATGWIFLAAGTVNLVRL
                     LRWYGWLTWREPLVLSLHVGYGWLALSMLVLGCALLGIGLAKEDAVHALTTGAVGSMT
                     LAVMTRASLGHTGRPKHAGPATVFIYGLVILGAMLRVFGPGTGLPTNLVLGSAGAAWS
                     GAYLLFALVYGPYLVRPSLDE"
BASE COUNT          772 a         1018 c          941 g          465 t
ORIGIN      
        1 agaaacactg atcagtgccc gcgcaatgct ggccatgatg aactccttca actattgata
       61 tcgtgtgggt gggaaaccat gagctagacg aaaagcgccg tgacaatacc agatggctca
      121 cggccactgc aagccggaca cacctgaact cgaccccgaa cggcacggct gcgcgacgcg
      181 aaatcacccg gcagacgccg gcctctgctg aagaatccag gctagcatga gtatcggcga
      241 tgtgataccc ttccgccgat agagatgcgc caacatgagg tgacgcatca gcttaatcaa
      301 gtaacactcg aagtcttgaa acgacaccag cccacggcga ctcgatgccg cagcggggcc
      361 tcgtgcctga gacgataaag gcttgcgcat gtcgcgcgga attattttcg gcgtaggact
      421 cttcgccctc acattattgg cgttcctctg tatccctcga cacctgccgg tgacgtcgtc
      481 ggcgaccggt cgctccgcgt tcagcgccca cattgaaaac ggccagctga cactgtccgg
      541 cacggtagcc agcgaggagg ccaaaggcgc cgcggtggcg cgcgcacaag atctcgccaa
      601 gagcctcagg ctgcgtgtca ccgacaatct gaacatcatc gaagacggcc aggccgctgg
      661 atgggaagcc gccctccccg cgctcgtcac acaggcggtc acactccagc accatcaggc
      721 caccctctcc ctctccggcc agacggtgac ggtaaaaggc gcagtcccaa gcgcggacgc
      781 aaaaacaaaa ctgctccatg aagtggcttc actgctcgga acctctatac acgtgcaaga
      841 ccaggtgacc gtcgcgtcgt caggctccgc gagtcaggcc aaccttactc ccgccagcca
      901 gccgtccgcc gcaccgcatg cctcccgagc ccaagtccaa gccgggctgg atgagatatt
      961 gcggggcgaa catatcgcat tcgaaagtaa cagcgcggtc ctgacttcga agggacgagc
     1021 cgtggtcgac aaactggtgc cggcgctcaa gcgggcaccc gatgcagtca tcgaaatcgg
     1081 cggccacacc gactcctacg gcgatcccga ctacaacctc caactgagcc gcaccagagc
     1141 cgaggcggtc cggcaatacc tcgtggaaca tcaggtaccg aatcggctga ccgccgtcgg
     1201 ttatggatcg acgcgccccc tgagccagga ccggacgcgc gccgcgtcga agaaaaaccg
     1261 gcgcatcgaa tttcgtgtga aagaggaacg gtgaacctat gggagccatg attctgcaaa
     1321 tggtggggtg cttgctggtg gcagcgacca tcggactgat gatgggctgg ctgctccgca
     1381 gctttgccac ctctgaaaaa cgtcagcagc tgtccgaaat cgccacccga ctccgcgggc
     1441 gcgaacatga actggatacc ctcaaccatg agttgaaggt gcgcacctct gccgtccaga
     1501 tgctggaagg caagatgatc acctcggaag cggcgctcaa agacctgacc gccgacctcg
     1561 ggacaaaagt cgaacaactc acggccttgc agacggaggt tcgtgaaaag ggtgtgcgcc
     1621 tgcatgccgc cgaacgtgaa cgggataccc tccgacgcga ggtcgaagaa gccgaagcca
     1681 ggatcaaggc acaatcgacg ggatttgccg aaatgcaggc gcagatggag aatgccgaag
     1741 acgtgttgat tgcccgcgac caggagattg ctaccttaaa aacctgggtg gagcaactgg
     1801 cgccgaaaga tgccgagatc gcccggctgc gcacccgtac ggaagagttg gaaccgttcc
     1861 tggaaaaaag ccgccgattg gagcgcgaac aggagcagga tcgcgcccgc gcgtcagcca
     1921 tactacaggc caaagaacag caactcgcac agacccaggc acgcctcaac gcaatcgaac
     1981 tggacctcgc caacctgcga acgcagagcc aggaagacct cgcgcgattc ggagagacat
     2041 tgcaggaacg ggacgcggag attcagcgtc tgcggacaac ggtcgaagag ttggacatgt
     2101 tccggggcga ggtggagaaa aaagaccttg cgctccgcga ggccgaagag cgtcgtgtga
     2161 tggatgtgag cgagcgggaa gaagagattg gagcactccg caagcgactc gtcgagtatc
     2221 gtgtagccca gcggttcagc gcgcaagcta agtcgacggg agccaccgag tcgggctcaa
     2281 ccggtagcgc caccacaaaa cggagcgcag gaaaaggtca cccggcccaa aaagacgatc
     2341 tcaaacagat ccacgggatc ggcccggtga tggagcgcgt cctcaatcgc atggggatgt
     2401 tcacgttccg gcagatcgcc gagtggaagg aacaagacgt cgaacacatg gcatcggaac
     2461 tcaacacgtt ccccgatcgc atccgacacg acaattggat cgacggcgcc aaggagcagc
     2521 atttcctgaa gtacggcgag gaactctaac cggcagcacg gtcctccctc cgggttcccg
     2581 accacctgca ccgcgtcaac cacctcaaca cgttgccgat cggcaggccc ccggcacctc
     2641 ccccctcccc tcactcatcc aggctgggtc ggacaagata cggcccatag accagagcaa
     2701 acagcagata ggccccgctc cacgcggctc cggcggagcc aagcaccaga ttcgtcggaa
     2761 ggccggtgcc tggtccgaag acacgcaaca tcgcgccgag gatcaccaac ccatagatga
     2821 agaccgttgc gggaccggca tgtttgggcc gccccgtgtg gccgagactt gcacgcgtca
     2881 tcaccgccag ggtcatcgat ccgacggcgc cggtggtcaa tgcatgcacc gcatcttctt
     2941 tcgcgagccc gattcccagc agagcgcacc cgagaaccag catcgacaag gcgagccaac
     3001 cgtaccccac atgcagactc agcacgagcg gttcccgcca tgtgagccag ccgtaccagc
     3061 gcaagagacg aacgagattc acggtcccgg cagcgagaaa gatccagccc gtcgccgacg
     3121 ccaacggcag caccgtccag gccagcgcgg ccatgaggac cagcgcgatc gagagtgcgt
     3181 cgaagcggga aaacgg
//
LOCUS       DAOQRM010000236         3188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_34455_length_3188_cov_15.407597, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000236 DAOQRM010000000
VERSION     DAOQRM010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34455_length_3188_cov_15.407597"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKK81_12820"
     CDS             <1..181
                     /locus_tag="PKK81_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV33816.1"
                     /translation="RAQYPSLPVIVLTGYHDEQLASSLRKQGVVECLEKPAEREQILT
                     SVAQAIAGRRISFYP"
     gene            324..992
                     /locus_tag="PKK81_12825"
     CDS             324..992
                     /locus_tag="PKK81_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33817.1"
                     /translation="MCHFIVRSSPHIPLRHLVAASLVGLLFIGLAPSPLNAARGGGKT
                     EVLPVQKAAPAAVIIEGLGNEAELRRQMRAKNGVAELGASTAQVMFSQAPTGTFGFIV
                     PKLLGLALVTQSPDLAVARVAPAENAYEIHKLADGSGMLVGFMGAELMTQVAPSQRPK
                     NVRIALYSNPSEKAPHIVAVPLVKLVADRMPIRLDAKNPNGPVLLDMDLQGSSTRAST
                     QGGQ"
     gene            complement(1067..1405)
                     /locus_tag="PKK81_12830"
     CDS             complement(1067..1405)
                     /locus_tag="PKK81_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33818.1"
                     /translation="MKRLVLSACALTIGATLALNSWCAAENTTTINILSPRNGEKVSE
                     TFDIKYEILNAPAGNHAHVYLDGAYQKGFKGTFTSVAKGEHTITVKIADHDHKDAGTA
                     TDSITVKVGE"
     gene            complement(1416..2045)
                     /locus_tag="PKK81_12835"
     CDS             complement(1416..2045)
                     /locus_tag="PKK81_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNV33819.1"
                     /translation="MFKRSTPGQIRLGRHNLAGDGQADLVHHGGVDKAVCAYPSEHWL
                     YWRGILPPHRLTGGAFGENFTLEGLTEADVCIGDVFSAGTAVVQISQPRQPCWKLARR
                     WQIKDLAVQMERTGFTGWYVRVIQEGTVEPGTPLQLMERPCPEWTVATANRIMHHERN
                     NLAAAEHLSLCPLLSASWQQTLRQRCHNQAGPDPQHRRFGTTHSSSSSF"
     gene            2279..3175
                     /gene="cynR"
                     /locus_tag="PKK81_12840"
     CDS             2279..3175
                     /gene="cynR"
                     /locus_tag="PKK81_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HNV33820.1"
                     /translation="MEFRHLRYFLAVADALHFTKAAERLPVSQPALSAQIKQLEQEVG
                     VPLFDRVGRSVQLTRAGSIFREHARRALREMELAQVAIAEEEGLQRGTLTVGVVQTVN
                     AYLIPEIVGRFSTLHPLVSLKLDELSGPDIEAGVKSGSLEVAIGFVPVVSDRIESQPL
                     FEEDFVLIASPRHRFAGRRHLSLSALAQESLILLPNMFCTRRLLDGCFEQAGVQPKII
                     VEMNSIEGILATVRRSNLATVLPRLSLGLARNQTLRAITLKNPTPRRGIGLLWKKGGY
                     RSGAAKALTDQVRAVVAEHWRS"
BASE COUNT          563 a          925 c         1010 g          690 t
ORIGIN      
        1 ccgcgcccaa tatccttccc tcccggtcat cgtgttgacg gggtatcacg atgagcagtt
       61 ggccagttcg ctacgcaagc aaggtgtggt ggagtgtttg gagaaaccgg cggaacggga
      121 gcagattctc acgtctgtgg cgcaggctat agccggacga cggatcagtt tttatccgtg
      181 aggtgcggtc tggagaagga gcgaaccccg tcgcgtctcg ccaggtctgt tcggttcacg
      241 tccttcctgt ttgattttcc accggcagct tcttagaatc ctccgtgcgc acacacatcg
      301 cttgatcagg aggaccagca ttcatgtgtc acttcattgt ccgatcgtct cctcacatcc
      361 cgctccgtca cctcgtggct gcaagcctgg tcggcctgct gttcatcggt ctggcccctt
      421 cccccctcaa tgccgctcgt ggtggaggca agaccgaggt gttgccggtg caaaaagccg
      481 cgccggcggc ggtgatcatt gaggggctcg gtaatgaagc ggagttgcga cgccagatgc
      541 gcgccaagaa cggggtggct gaactcgggg cttcgaccgc gcaagtgatg ttttcgcaag
      601 cgccgactgg gacgtttgga ttcattgtgc cgaagctcct aggcctggcc ttagtgacgc
      661 agagcccgga tctcgccgtc gctcgggtgg cgcccgcgga aaatgcctac gagattcaca
      721 agcttgccga tgggagtgga atgttggtcg gattcatggg cgcggaactg atgactcagg
      781 tggcgccgag ccaacgcccg aagaatgtcc gtatcgcgct ctattccaat ccgtctgaaa
      841 aggcaccaca tatcgtggct gtgccgctgg tcaagctggt ggcagaccgg atgccgatcc
      901 ggctcgacgc gaagaatccg aatggtcctg tcttgctgga tatggatcta cagggcagct
      961 cgacgcgtgc atcgacccaa ggtgggcagt agacaggtag gctattggcg gggaggcggc
     1021 atcgttgcgg cctccccgat cgcttttctc gtgacgcacc ccggtcctac tctcccacct
     1081 tcacggtaat gctatctgta gcggttccgg cgtccttatg atcgtggtcg gcgatcttca
     1141 ccgtaatcgt atgctccccc ttggccacgc tggtaaacgt gcccttgaag cccttctggt
     1201 acgcgccgtc gaggtagacg tgcgcgtgat tgcctgccgg cgcgttcagg atttcatact
     1261 tgatgtcgaa ggtctccgag accttctcgc cattgcgcgg ggagaggata ttgatggtag
     1321 tcgtattttc agcggcacac caggaattca gggcgagggt ggcgccgatg gtcagtgcac
     1381 aggcggagag aaccaatcgc ttcatggaaa cctcctcaga atgatgatga cgaagaatga
     1441 gtcgtgccga atcgtcgatg ctgcgggtca ggccctgcct gattgtggca tcgctgccgc
     1501 aaggtctgtt gccagcttgc ggaaagtaac gggcagaggc tcaggtgttc ggccgcggcc
     1561 agattgttcc gttcatggtg catgatccga ttggcggtcg cgaccgtcca ttctgggcag
     1621 gggcgttcca tgagctgcag gggcgtgccc ggttcgaccg tgccttcctg gatgacccgg
     1681 acataccagc ctgtgaagcc ggtgcgttcc atttgtaccg ccaaatcttt gatctgccag
     1741 cgacgggcga gtttccagca gggctgtctg ggttgagaaa tctgcacgac ggccgtaccc
     1801 gctgaaaaga catcgccgat gcagacatcg gcttccgtga gcccctccag cgtgaaattc
     1861 tcgccgaatg cgccgccggt gagacgatgg ggcggcagga tcccgcgcca atacaaccag
     1921 tgctccgacg gataggcaca gaccgccttg tcgacgcctc cgtgatggac gagatccgcc
     1981 tgtccatctc ctgccagatt gtgccggcca agtcggatct ggcccggggt agagcgcttg
     2041 aacatcccgg ttgtccaggg cgttgagtcc ggatcgtccg cacagtcgct gtgctcactg
     2101 gacgggagcc ccacctggat cgatcgtatg agcccgcttg tgttctgcac agccccgtcc
     2161 cttccgccac gagttttccg cttttcccgg ccgcagtcaa cctagtgccc gggacgagaa
     2221 ggcgtcaaag cgtttgttct gattagtatc atcacttcca tttatgataa aacgcggcat
     2281 ggaatttcgc catctccggt attttctcgc cgtggccgat gcgctgcatt tcaccaaggc
     2341 cgccgaaagg ctgccggttt cccagccggc gttgtccgcg cagatcaagc agctggagca
     2401 ggaagtgggg gtgccgctgt tcgatcgcgt ggggcgatcg gttcaattga cgcgggccgg
     2461 atcgattttt cgcgagcatg cccggcgggc cttgcgggag atggaactgg cgcaagtggc
     2521 gattgccgag gaagaggggc tccagcgggg gaccctcacg gtcggtgtcg ttcaaaccgt
     2581 gaacgcctat ctgattcccg aaatcgtcgg ccgcttctcg accctgcatc cgctggtgtc
     2641 gctcaagctc gacgaactgt ccggtccgga catcgaggcc ggcgtcaaga gcgggtcgct
     2701 ggaagtcgct atcgggttcg tgccggtggt gtcggaccgc atcgagagcc agccgctgtt
     2761 tgaagaagac tttgtgttga tcgcctcccc caggcaccgg tttgccgggc gccggcacct
     2821 gtccctgtcg gccctggcgc aggagtcgtt gattctgctg ccgaatatgt tctgcacccg
     2881 acgcttgttg gatgggtgtt tcgagcaggc cggtgtccag cccaagatta tcgtggaaat
     2941 gaattccatc gaggggattc tggcgaccgt tcgcaggagc aacctcgcca cggtcttgcc
     3001 gcgtttgtct ctcgggctgg cgcgtaacca gacgctccga gccattacgc tgaagaaccc
     3061 cacacctcgt cgaggcatcg ggctcttatg gaaaaaggga ggctacagga gcggggcggc
     3121 caaggcgctg accgatcagg tgcgggccgt tgtggctgaa cattggcgtt cgtgagcgcg
     3181 ttcgccgg
//
LOCUS       DAOQRM010000237         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_34543_length_3183_cov_15.213875, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000237 DAOQRM010000000
VERSION     DAOQRM010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34543_length_3183_cov_15.213875"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..2918)
                     /locus_tag="PKK81_12845"
     CDS             complement(144..2918)
                     /locus_tag="PKK81_12845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV33821.1"
                     /translation="MSVAVRWFHAVRSPLYSGMTERQWYACCAVILVVTAAIDAYTPL
                     GIAAGVGYLLAVVVAGMVCEVRFPWRVAAVSIGLTVLGFFWSPPGGVVWIGATNRVGL
                     AMAILLTAWIVWQWNMAVRGRSHDLAVRLQSILTHSQTSIFLKDLQGRYLEVSEQFLS
                     LTGFSAEMVIGKADRDIFPPELVAIYREHDAQVIKAGAAMNFEETVPLGDGLHLNIVC
                     RFPLRDGQGSIVAIGGIATDITARLQAEAARSEAQERFDLVVAATQTGIWDWDLHTNR
                     MYYCPLWKASLGYQEHEISNSPTEWESRLHPDDKARACALVDDYLSGRIGAYELEHRL
                     RHKDGSYRWIQTCALLQRDADGRPRRMTGSHVDITERKRAEDALTQSESTLRSFFNSG
                     AMMMGIVELLDGDILHVSDNRCVASFFGTTPELMQGRLASELGAPPDILALWHRSYEE
                     SERTKQPVRFEYAHPQKGSGVELRLSATVCWIGIGPSGRSRYAYVVDDVTEARCLAEA
                     LGQTAERYHGVVTALAEGVVLHDEQGRIIACNPSAERILGLTADQLMGRTPVDPGWQA
                     IHEDGSHFAPDQRPPMVTLRTGRPCSNVIMGLSRPTGEQRWISINTQAILRAGDARPY
                     AVVGSFHDITERRRAEQALLEVQSQLEQRVRERTARIQQLESQRSQAEKLAALGHLAA
                     DIAHEVNNPLAGITNAFHLVKQGIGSEHRHYRFVDLIDREIQRLTAIVKKMYTLYQGL
                     PATERQATDIAELLQDVATLLEHKMAVKRLRLHTEVDTPLQVVNVSRSDLFQVLLNLV
                     QNAIDASPPDSEVVLNVSEREGLLRWSVTDCGTGIAPDVLPRMFEPFFTTKPGAGWQG
                     LGLGLSVSRGLVQAMGGRIEVQTELQRGSTFTVVYPLEPPDMSGERAAGVNADKEQKD
                     DDYATTHSHC"
BASE COUNT          669 a         1016 c          861 g          637 t
ORIGIN      
        1 ccggcatccg gacatcggcc agcagcaagt cgtagccgtt ttccagcagg ctttcggcct
       61 cagccgcatc tcgggcagca ctacacgcgt atcctgcctc agcgagaatt tccgccgtag
      121 attcccgaaa cgtctcttcg tcatcagcaa tgagaatgcg ttgtggcata atcgtcatct
      181 ttctgctcct tgtctgcatt cactcccgcc gctcgctccc ccgacatatc cggcggttcc
      241 aagggataca ccaccgtgaa cgtcgaccca cgttgtaatt cagtctggac ttcgatccgc
      301 ccccccatgg cttgcaccag cccacgcgac acagataacc cgagtccgag tccctgccaa
      361 ccggcaccag gcttcgtcgt aaaaaatggc tcgaacatac ggggaagtac gtccggagct
      421 atccccgtcc cgcaatcggt cacgctccat cgcagcagcc cctcgcgctc ggacacgttc
      481 agcaccacct cactgtcggg gggagaggca tcgatggcgt tctgcacgag attgagcaac
      541 acctgaaaga gatcggagcg cgacacgtta accacctgaa ggggtgtatc cacctctgtg
      601 tgtaaccgga gtcgtttcac ggccatctta tgctccagca acgtggccac gtcctgaagc
      661 aattcggcta tgtcggtggc ctgtcgctcg gtcgccggga ggccttgata cagggtatac
      721 atctttttca cgatcgcggt aaggcgttga atttcgcgat cgatgagatc cacgaatcga
      781 tagtgtcgat gctcgctccc gattccctgc ttcaccaggt gaaacgcatt ggtaatccct
      841 gccaggggat tgttgacttc atgggcgata tcagccgcga ggtggcccag cgccgcgagt
      901 ttttctgcct gagaccgctg ggattcaagt tgctgaatcc tcgccgttcg ctcacgcacg
      961 cgttgctcca gctgactctg cacctccagc aaggcctgct ccgcccgacg gcgctcggta
     1021 atgtcatgaa aagagccgac gacggcgtaa ggtcgcgcgt caccggctct caggatcgcc
     1081 tgcgtattga ttgagatcca tcgctgttcc ccggtcggtc gagagagacc cataatcaca
     1141 ttcgagcacg ggcgaccggt ccgaagcgtg accatcggcg ggcgttgatc aggagcaaag
     1201 tggctcccat cctcatgaat cgcctgccat cccggatcga ccggcgtccg ccccatcagc
     1261 tggtcggccg tgagcccaag aatccgctcc gcgctcggat tacaggcgat aatcctccct
     1321 tgctcatcat gcaggacgac accttctgcc agcgcagtca ccaccccgtg gtaccgctcg
     1381 gccgtctggc ccagcgcttc tgccaagcat cgtgcttccg tgacatcgtc caccacgtaa
     1441 gcataacgcg accgcccgct tggtcctatt ccaatccagc agacggtggc ggaaaggcgc
     1501 agttcgaccc cgctgccttt ttgcgggtgg gcatattcga atcgcaccgg ttgtttcgtc
     1561 cgttcgctct cttcgtacga tcggtgccaa agagcgagga tgtccggtgg ggcgccaagc
     1621 tctgacgcga gtcgcccctg catcaattcc ggagttgtgc cgaagaagct cgccacacag
     1681 cggttgtctg agacatgcag aatatcgccg tcgagcagct ccacaatccc catcatcatc
     1741 gcgccgctat tgaagaaact tctcaatgtg gactcactct gggtcaacgc gtcttcggct
     1801 cgcttccgtt cggtaatatc cacgtgcgag ccggtcatcc ggcggggccg tccgtcggca
     1861 tctcgttgca acagagcaca ggtctgaatc caacggtagc tgccgtcttt gtgacgcagg
     1921 cggtgttcga gttcgtatgc gccgatgcgc ccggacaaat agtcgtccac aagcgcacat
     1981 gcacgagctt tgtcatcggg atgcaatcgg gactcccact ccgtcggtga gttggaaatc
     2041 tcgtgctcct gatatcccag gctcgccttc cacagcgggc agtaatacat gcggttggtg
     2101 tgcaggtccc agtcccaaat tccagtctgt gtcgcggcca caacgagatc gaaacgttcc
     2161 tgagcctcgc ttcgagcggc ctctgcctgc aatcgggcgg tgatatccgt ggcaatcccg
     2221 ccgatggcca caatcgatcc ctgcccgtca cgcaacggaa acctgcaaac gatatttaag
     2281 tgcaggccat cgcccagcgg cacggtctct tcaaaattca tggccgcacc ggccttgatg
     2341 acctgggcat catgttcccg atagatagcg accaattcag gggggaaaat gtcccgatca
     2401 gctttcccga tcaccatctc ggccgagaag ccggtcagcg acagaaactg ttcactcacc
     2461 tccaggtatc gcccctggag gtctttgagg aagatacttg tctggctatg tgtgaggatg
     2521 gactgcaacc gaacagcgag atcgtgggag cgaccgcgaa ccgccatgtt ccattgccag
     2581 acgatccagg cggtcaggag gatggccatg gcgaggccaa cgcgattcgt cgccccgatc
     2641 catacgacac cacccggtgg agaccaaaag aatcccagga cggtgagtcc gatcgaaaca
     2701 gccgccacgc gccaaggaaa tcgtacctcg cataccattc cggccacgac cacggcgagc
     2761 agatacccca ccccggccgc aatccccaat ggcgtgtacg catcaatggc cgccgtgacc
     2821 acgaggatga cagcacagca ggcataccat tgcctttcgg tcatgcctga atacagcggt
     2881 gaccggacgg cgtgaaacca gcgcacggct acactcatca cagaacgact ccgtacgcta
     2941 ctcaggcgta acgacaccgg atcatggggc gggtaatagc tgcagcttcg tggtcgagat
     3001 cccgacggca gcgacgatcc tacctgccgg ccctacagcg atcctctccc acgacaagaa
     3061 ccatcctcca tgaagccgca agaagcttta tatgtgagat ggctgtactg tctggaaaga
     3121 atgttcccga caaggttgag ccaacgaata accgggagaa gtgtggtgtc cccaacggga
     3181 ttt
//
LOCUS       DAOQRM010000238         3081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_36366_length_3081_cov_14.694316, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000238 DAOQRM010000000
VERSION     DAOQRM010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36366_length_3081_cov_14.694316"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PKK81_12850"
     CDS             <1..1047
                     /locus_tag="PKK81_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV33822.1"
                     /translation="LTTEEIKSAGIIVQPVTRSEFRTIRDFPGTVEPNEHALAEITTL
                     VRGRVIDVYADLGREVKGGTLLALLYSSELGMAQSAYLKATAKLNVAERAFRRAELLL
                     KEKVIGVAELQRREGEMLSLRAELREARDRLLILGLTDEDLRNLDRNHTIRSHVPVVA
                     PFDGRIIARNLTKGEVVETTEKLFVVADLTDVWVTAVIPEKDIPYIRPDQTGTGQSVE
                     VHVAAYPGQAFQGRITYVGDVLDPATRTMRLRLELPNPERKLKPAMYATVRVYSEPEA
                     NALLIPESAVQRDRDRQFVFVEREPAIFEARDVKLGSSNGREIKVLDGLLEGESIVTN
                     GAFVLKSELLGEQM"
     gene            1052..>3081
                     /locus_tag="PKK81_12855"
     CDS             1052..>3081
                     /locus_tag="PKK81_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNV33823.1"
                     /translation="MLSSLLAFALRQRVLVVVMACILTAGGIYAFRTIAIDAFPDVTT
                     VLVQVVTKVPGMSPAEVERFVTFPMELQLMGAPGLTDIRSFSKVGLSMITVVFKDDID
                     IYLARQVVLERILEIQELLPPGSSSQLVPNTTGLGEVYQFYLEGRHDNDKDFVMSDSD
                     LMERRTIEDWVIRPLLKGLPDVVDVNSLGGFVKQYQVMVEPGLLRKYGLALHDVFDAV
                     AKNNANAGGNILEKDDEKYVIRGVGLIKTLEDIDHIVVKQVGGTPVYVRDVAEVRIGH
                     AVRHGAAVLNGKHEVITGIVLMLRGGNARDVVQSIKDKISEIHHKRLLPDGLRIIPFY
                     DRIELITAALDTVYKALLEGIVLVVVILFLFLGNVRSALIVTATLIVTPLVTFIIMDQ
                     VGLTANLMSLGGLVIAIGMMVDGSVVVVENVYRHLAEQRDDRLSKTEVILHAVTEVGQ
                     PVIFGILIIILVFLPILTLQGMEGKMFQPLANTIIIALLVSLVLSLTLSPVLCAMALK
                     RGTEEDTFLLRWAKWAYLPTLRWAMGHRIAVLSIALGLLGASLSLFPFLGGEFIPILN
                     EGSISPQTIRYPSIALDRSIEIEKEMQRAVMEFPEVRMVVSKIGRSEMGNDPQEPNAS
                     DPTVTLRPMDEWTTAKTKAGLDDAIRKRIEKVPGANYLLSQPIQQRVDELLSG"
BASE COUNT          654 a          909 c          877 g          641 t
ORIGIN      
        1 ttgaccaccg aggaaattaa atcggcgggc atcatcgtcc aacccgttac acgctcagag
       61 tttcgcacga tccgtgactt ccccggcacc gtcgaaccga atgaacatgc cttagccgaa
      121 atcaccacgc tcgtccgcgg gcgggtcatc gacgtctacg ccgacctcgg cagagaagtg
      181 aagggaggga ccctgctggc tctgctctat agcagcgaac tgggtatggc gcaatcggct
      241 tatctgaaag ctacggccaa gctcaacgtc gccgagcgcg ccttccgccg ggcggaattg
      301 ctcttgaaag aaaaggtcat cggcgtggcc gagttgcaac gacgcgaagg agaaatgctc
      361 agccttcggg ccgagctgcg agaggcccgc gaccgcctct tgatcctggg tctcacggat
      421 gaagacctgc gcaacttaga tcgcaatcac acgatccgct cacacgttcc ggtggtggct
      481 cccttcgacg gccgcatcat cgctcgtaac ttaaccaaag gcgaggtcgt ggaaaccacg
      541 gaaaaactgt tcgtggttgc cgacctcacg gatgtctggg tgacggcagt cattcccgag
      601 aaagatatcc cctacatccg gcccgatcag acaggaacag gtcaatcggt ggaggtccat
      661 gtggccgcct accccggaca agccttccaa gggcgcatca cctatgtcgg agacgtgctc
      721 gaccccgcga cccggacgat gcgtctccgg ctggaattgc cgaatcccga gcgtaagctc
      781 aaacccgcca tgtatgccac cgtgcgcgtc tattccgaac cggaggcgaa cgcgcttctc
      841 attcccgagt ccgctgttca acgcgaccgt gaccggcaat tcgtctttgt cgaacgcgag
      901 cccgccatat tcgaggcgcg cgatgtgaaa ttaggcagtt cgaacggccg tgagattaag
      961 gtgctggacg gactgctgga gggagaatcc atcgtgacga acggcgcctt cgtgctaaaa
     1021 tccgaactgc tcggggaaca gatgtaaccc gatgctgtcc tcccttcttg ccttcgctct
     1081 ccgtcaacgt gttctggtcg tcgtcatggc gtgcatcctg accgccgggg ggatctatgc
     1141 cttccgcacc atcgccatcg acgccttccc ggacgtcacc actgtgctgg tccaggtcgt
     1201 cacgaaagtg cccggcatgt ctccggccga agtcgaacgt ttcgtcacct tccccatgga
     1261 actccagctc atgggcgcgc cggggctgac ggatattcgc tcgttttcaa aggtcggcct
     1321 gtcgatgatc accgtcgtct tcaaggacga tatcgatatt tatttggccc ggcaggtcgt
     1381 gctcgaacga atactggaaa tccaggagct gctgccgcct ggctcctcat cccaattggt
     1441 tccgaacacc accggattgg gggaagtcta tcaattctat cttgagggac ggcacgacaa
     1501 cgacaaagac ttcgtgatga gcgattcgga cctcatggag cggcggacga ttgaggactg
     1561 ggtcattcga ccgctgctca aagggctacc cgacgtggtg gatgtgaatt cgctgggagg
     1621 gttcgtcaaa cagtaccagg tgatggtcga gccgggtctg cttcgtaaat acggtctggc
     1681 gcttcacgat gtgttcgatg ccgtggcgaa gaacaacgcg aatgccggcg ggaacatcct
     1741 ggagaaagac gacgaaaaat atgtgattcg cggtgtcggg ctgatcaaga cgctcgaaga
     1801 catcgaccac atcgtcgtca aacaggtggg tggtaccccc gtctacgttc gggatgtggc
     1861 ggaggtccgg atcgggcacg ccgtccggca cggcgccgcc gtgctgaatg gaaaacacga
     1921 agtgatcacc ggcatcgtgc tgatgctgcg tggcggcaat gcccgggacg tggtgcaatc
     1981 catcaaggac aaaatctcag agattcatca caaacgcctg ctgcctgacg gattgcgcat
     2041 cattccattt tacgaccgga tcgaactgat tacggcagcg ctggataccg tctacaaagc
     2101 gctgcttgag ggcattgtgc tcgtggtcgt cattctcttt ctgtttcttg gcaacgtacg
     2161 aagcgccctc atcgtgaccg ccacactgat cgtgacgccg ctggtcacct tcatcatcat
     2221 ggaccaggtt ggactgacgg ccaatctcat gtcactcggc ggactggtca tcgctatcgg
     2281 catgatggtc gatggctcgg tcgtggtggt agagaatgtg tatcggcacc tcgccgaaca
     2341 gcgagacgac cgcctcagta aaaccgaggt cattttacat gccgtcaccg aagtcggaca
     2401 accggtgatt ttcggcatcc tgatcattat ccttgtcttc cttccgattc tgacgctgca
     2461 gggcatggaa ggaaaaatgt ttcagccgct cgccaatacg atcatcatcg cgttactggt
     2521 gtccttggtc ttgtcgctga cactctcccc tgttctctgc gcgatggcac tgaagcgagg
     2581 aacggaagag gacacgttcc tgctccgctg ggccaagtgg gcctatctcc cgacattgcg
     2641 ctgggcgatg gggcatcgca tcgccgtgct gtccatcgca ctcggcctcc tgggcgccag
     2701 cctgtccctc tttccctttc tcggaggaga atttattccc atcctgaacg agggatcgat
     2761 ttcaccccag accattcgct atccgagcat cgcgctggat cgctccatcg agattgaaaa
     2821 agagatgcaa cgcgccgtga tggagtttcc cgaagtgcgc atggtggtgt ccaagatcgg
     2881 ccgctcggag atgggaaacg atcctcagga acccaatgcc agtgacccca ccgtgactct
     2941 ccgtccgatg gacgaatgga ctacggccaa aaccaaagcc ggactcgacg atgccatccg
     3001 caaacgcatt gaaaaagtgc cgggagccaa ttatctgctg agccaaccga tccaacaacg
     3061 cgtggatgag ctgctgtccg g
//
LOCUS       DAOQRM010000239         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_37444_length_3027_cov_20.168910, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000239 DAOQRM010000000
VERSION     DAOQRM010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37444_length_3027_cov_20.168910"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKK81_12860"
     CDS             <1..417
                     /locus_tag="PKK81_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33824.1"
                     /translation="AQEQPAVEELILIAPAFNMMDLRAAQISNERREQWQATGSMPWD
                     DEPMHREAPIPWHWVEDSQALWSRRLTMPRRVKTTILHGLQDTVIKPDGSWTFVQHLL
                     SQDPEFPIDVLFKTGDHRLSSPDHLETFRQLVSRSR"
     gene            428..595
                     /locus_tag="PKK81_12865"
     CDS             428..595
                     /locus_tag="PKK81_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33825.1"
                     /translation="MLVLIHKECGGPALDESPVGEVCAIPVSQFPFTCFTCLEDILDE
                     SEVRLTEDLGI"
     gene            695..1501
                     /locus_tag="PKK81_12870"
     CDS             695..1501
                     /locus_tag="PKK81_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HNV33826.1"
                     /translation="MSSTQQHNSGPDSDGPEVPEPSHAERAKTLVYLQQTGGLSTISR
                     KQPGWPFGSVMPYGLDDQGQPLFLISTMAMHTQNLLGDPRASLLVTPPESRTDPLGAA
                     RVTLMGSVTRVPKEESAPVRERYLARHANAAYWVDFNDFGFFRMAIADIYFVGGFGSM
                     GWVVPSDYTAAAVDPLADQASGLIREMNEQQTATLLLLARVYGKLEAQQVTMTALDRL
                     GFHLRIKTAERMQGGRVAFTNPVRTAAEVRAGLADMAARAKAGAELVHSL"
     gene            1559..>3027
                     /gene="ettA"
                     /locus_tag="PKK81_12875"
     CDS             1559..>3027
                     /gene="ettA"
                     /locus_tag="PKK81_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNV33827.1"
                     /translation="MATNDKQVIFSLVGVGKVYPPKKQVLRDIYLGFYYGAKIGVLGL
                     NGSGKSSLLKIIAGTDPNYVGEITRSKGYSVGLLEQEPQLDLNKTVKEVVEEGKKELV
                     ALLHEYEAVSNSMGEAGPDEMEKLIDKQAQLQEKIEAANGWELESELEIAMDALRCPP
                     ADQKISVLSGGEKRRVALCRLMIQEPDILLLDEPTNHLDAESVQWLEQHLQQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGSGIPFQGNYTAWLEQKQDRLEKEEKAESKRKKTLEH
                     ELEWIRMSPKARQSKGKARLNRYEELVNQKQDQLAADLEIYIPPGPRLGDLVVEAKGI
                     SKAFGDNVLYENVEFSLPKGGIVGVVGPNGAGKTTMFRMIIGKEKPDTGTIRIGETVK
                     LGYVDQDRSLDPNKTVYEIISEGQDTIMLGKAEVNARGYCARFNFAGTDQQKKVKDLS
                     GGERNRVHLARMLKEGANLIILDEPTNDLDVNTLRALEEG"
BASE COUNT          666 a          876 c          946 g          539 t
ORIGIN      
        1 gcgcaggagc aaccggcggt ggaagaattg attctcatcg ccccggcgtt caatatgatg
       61 gatctgcgcg cggctcagat ttccaatgag cggcgtgagc agtggcaggc caccggctcc
      121 atgccatggg acgacgagcc catgcatcga gaggcgccga tcccctggca ctgggtcgaa
      181 gacagccagg ccctctggag ccgtcgcctc accatgccgc gtcgcgtcaa gacgaccatt
      241 ctgcacgggc tgcaggatac agtgatcaag cccgacggca gctggacctt cgtgcagcac
      301 ctgctgtcac aggatcctga atttcccatc gacgtcttat tcaaaaccgg tgatcaccgt
      361 ctgagcagcc ccgaccattt ggagacgttc aggcagttgg tgagccgatc gcgatagagg
      421 ggcgcctatg ctggtcttga tccataaaga gtgcggtggc ccggcgctgg acgaatcgcc
      481 ggtcggcgaa gtgtgcgcga ttccggtgag tcagttcccg ttcacctgct tcacctgcct
      541 ggaagacatc ctcgatgaat ccgaagtgcg actcaccgaa gatctcggca tctgacggac
      601 gttgtgccgg tcccgttccc cccgcgctca gtggaaattc cggaagcatc caggtaaggt
      661 gtcgtgcagc ttattgcaca gaaaagaggc atccttgtca tcaacgcagc aacacaatag
      721 cgggccggat tccgacggtc ctgaggtccc tgaaccgtcc catgccgagc gggcaaagac
      781 tctggtctat ctacagcaga cgggcggcct ttcgaccatc tcgcgtaaac aaccaggctg
      841 gcccttcggg tccgtcatgc cctatggcct cgacgaccag gggcaaccgt tgtttctcat
      901 cagcaccatg gcgatgcaca cgcagaacct cctgggcgat ccacgcgcca gtctgctggt
      961 gacgccgccg gaaagtagaa ccgatcctct gggtgccgcc agggtgacgt tgatgggatc
     1021 ggtgacgcgt gtgccgaagg aggagagtgc gccggttcgc gaacgctacc tggcccggca
     1081 tgctaacgcc gcctattggg tggacttcaa cgacttcggc ttctttcgca tggcgatcgc
     1141 agatatctat ttcgtcggcg ggtttggctc gatgggctgg gtcgttccgt ccgactatac
     1201 ggccgctgcg gtggatccac tagccgatca ggcatctggg ctgatccgtg aaatgaatga
     1261 acagcagaca gccacgctgt tgctcctggc ccgggtgtac ggcaagctgg aggcgcagca
     1321 ggtgaccatg accgcgctgg atcgtctggg ttttcatctg cgcatcaaga ccgccgagcg
     1381 tatgcagggg ggacgggtcg cgttcaccaa tccggtccgc acagccgcag aggtcagggc
     1441 cggcctggcc gacatggccg cccgggcgaa ggccggggcc gaactcgtgc actcattgta
     1501 agcggccgcg caacgtattc ttctcaacac agtaccacac catcagatag gggaacgcat
     1561 ggcgacgaac gataagcagg tgattttttc tctggtcggt gtcggcaagg tctatccgcc
     1621 gaagaagcag gtcctgcgcg atatctacct gggcttttac tacggggcca agatcggcgt
     1681 gctggggttg aacggatccg gaaaaagctc gttgctcaaa atcattgccg ggaccgatcc
     1741 caactacgtc ggtgagatca ctcgctcgaa gggctacagc gtcggactgc tcgaacagga
     1801 accgcaactc gacctcaaca agacggtcaa agaggtcgtc gaggaaggaa agaaggagct
     1861 ggtggcgctg ctgcacgagt acgaagcggt cagcaacagt atgggcgagg ccggccccga
     1921 tgagatggaa aagctcatcg acaagcaggc gcagctgcag gaaaagatcg aggcagcgaa
     1981 cggctgggag ctggaaagcg aactcgaaat cgccatggat gcgctgcgct gcccgcctgc
     2041 cgatcagaag atcagtgtgt tgtccggcgg cgaaaaacgc cgcgtggccc tctgccgcct
     2101 catgatccag gagccggaca ttctgctgct cgacgagccg accaatcacc tcgatgcgga
     2161 atccgtgcaa tggctggagc agcacctgca acagtacaag ggcacggtca tcgcggtgac
     2221 gcacgaccgc tacttcctcg acaacgtggc ggggtggatt ctggaactcg accgcggctc
     2281 cggcattccc ttccaaggca actacacggc gtggctcgaa cagaagcagg accggttaga
     2341 gaaggaagag aaggccgaat ccaagcgcaa gaaaacgctc gaacacgagt tggaatggat
     2401 tcgcatgtcg ccgaaggccc ggcagtcgaa gggcaaggcg cgtctcaacc ggtatgagga
     2461 attggtcaat cagaagcagg atcagctggc cgccgatctg gaaatctata ttccgccggg
     2521 acctcggctc ggggatctgg tggttgaggc caagggcatc agcaaggcct tcggcgacaa
     2581 cgtcctctat gaaaatgtcg agttcagcct gccgaagggc ggcatcgtcg gcgtcgtcgg
     2641 tcccaacggt gcgggcaaga ccaccatgtt ccgcatgatt atcggcaagg agaagccgga
     2701 cacgggcacc attcgcatcg gcgaaacggt caagctcggt tacgtggatc aggatcggtc
     2761 gctcgatccc aacaagacgg tgtacgaaat catttccgag ggacaagaca cgatcatgct
     2821 gggcaaggcc gaagtgaatg cccgcggcta ctgtgcccgc ttcaatttcg ccggcaccga
     2881 tcagcagaag aaggtcaagg atctgtcagg cggcgagcgg aaccgcgtgc atctcgcgcg
     2941 catgttgaaa gaaggggcca acctcatcat cctcgacgaa ccgaccaacg acctggatgt
     3001 gaacacgttg cgcgcgttgg aagaggg
//
LOCUS       DAOQRM010000240         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_37579_length_3019_cov_15.727733, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000240 DAOQRM010000000
VERSION     DAOQRM010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37579_length_3019_cov_15.727733"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1513
                     /locus_tag="PKK81_12880"
     CDS             <1..>1513
                     /locus_tag="PKK81_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNV33828.1"
                     /translation="SELHWTSFAEFFRSRLYDPRLVTRNFLTYCDDDRNLRRTYSYAE
                     FGTIVERMAEMFHTKFGLTRGDRIATVLFNHDLTALIYFAAWTLGVAVVPINVEETPE
                     KKRYILEHSEASVALCWQDVYEELKALQSMLPALREVVALGDGGILDGKETRGKSRKN
                     SHAVGARFAPGTSRLDDPALIIYTSGTTGPPKGVILTVANLLIDADAIADRHGFGLTD
                     RLMCVLPVHHVNGIVVTLMTPFYCKGSVVLNRKFKTGSFWRRLHEEQVTSVSVVPTLL
                     EFLLDADEDLAAYRLDHFGGVICGAGPLLKDTALRFEERFGFPIRHGYGLSETTCYSC
                     FLPNDLTGEEHRHWLTDFDFPSIGTALRHNEMAILDESGKAVAEQARGEICIRGRTVC
                     AGYFKRDDANEVAFQWGWFRSGDEGFYIADDRGRPFFFISGRLKELIVRGGVNISPLE
                     IDDVLKGHPAVQFAMALPFENRYYGEEIAAYIVPKDPESPPTEADILSHCRGR"
     assembly_gap    1514..1613
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1614..2981
                     /locus_tag="PKK81_12885"
     CDS             <1614..2981
                     /locus_tag="PKK81_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNV33829.1"
                     /translation="LDSSGTHFLVNGEKLWCTNGPIARYLTLVARVPALRVECEGKVE
                     WIPAPQGQRADDHVHTAFILDMATPGVLVRQRCQFEGCRGIENAHIALKNVQVPIEQV
                     IGDIGKGLKYALTILNVGRGISIPAICLGMAKQAWQPTLDRANSRVTFQKPLAERQTQ
                     QIRIGDMAGHLFAMEALALLVWRLADQHRYDIRIEAAVAKIFCSEHTIRFIRDAQTIF
                     GGMGYETADSKHARGEAAFGIEQLVRDAEMYRIGEGATDILRPFVVREGLSPHLDRAK
                     RFYADGLSILEQARQAMTLMRFYLPWYLRQWRKRPLPDRREITHPQVRPAALYVERTS
                     RRLARAIFYALLRFQASFKDEQRLQNKIESVGEDLLAMLATVLYAERQTRLDGHTSVW
                     ELAEAFSAAAKQRIEQRLREFRHHRDTTAATTGRQALKGYYPTLSEGIIRRRLEDYRR
                     NQHMT"
BASE COUNT          593 a          891 c          862 g          573 t
ORIGIN      
        1 cctcggaact ccactggacc tcgttcgccg agttcttccg atcgcgcctg tacgacccgc
       61 gactcgtcac caggaatttc ctgacctatt gcgacgacga ccggaacctg cgccgaacct
      121 acagctatgc ggaattcggc acgatcgtcg aacggatggc ggagatgttt catacgaagt
      181 tcggcctgac ccgcggcgac cggatcgcca ccgtgctgtt caaccatgat ctgacggcgc
      241 tgatctactt cgcagcctgg accttgggag tcgccgtcgt gccgatcaat gtcgaagaaa
      301 cacccgagaa gaagcggtat atcctcgaac attcggaggc ctcggtcgcc ctctgctggc
      361 aggatgtcta cgaggaactc aaagctcttc agtccatgct gccggccttg cgcgaagtgg
      421 tcgccctggg ggacggtggc atccttgacg ggaaggagac caggggaaag agccgaaaaa
      481 acagccacgc cgttggagcc cgctttgccc cgggcacctc ccgactcgac gatcctgcct
      541 tgatcattta cacatccggg acaaccgggc cgccgaaagg cgtcatcttg acggtggcca
      601 atcttctgat cgatgcggat gccatcgccg accggcatgg attcggtctc accgatcgtt
      661 tgatgtgcgt cctgcccgtc caccatgtta acggcatcgt cgtcactctg atgactccgt
      721 tttattgtaa gggaagcgtc gtcttgaacc ggaagttcaa gaccggcagc ttctggcgac
      781 gactccacga agagcaggtg accagtgtca gcgtggtccc cacattgctt gagtttcttc
      841 tggatgccga cgaagacctc gccgcctatc gcctggatca cttcggcggc gtgatctgcg
      901 gcgccggacc gctgctgaag gacacggcct tgcgcttcga agagcgtttt ggattcccca
      961 tccgccatgg atacggtctg tctgaaacga cctgctattc ctgtttcctc ccgaacgacc
     1021 tcacagggga agaacatcgt cactggctca cagattttga tttcccctcg atcggaacgg
     1081 ccctgcgtca caacgagatg gccattctcg acgagtccgg gaaggcggtt gctgagcagg
     1141 cacgcgggga gatctgcatc cgcgggcgca cagtctgcgc cggctatttc aaacgggacg
     1201 acgccaacga agtcgccttc caatggggtt ggttccgttc aggcgacgaa ggcttttaca
     1261 tcgccgacga ccggggacgt ccgttctttt tcatttcagg ccggttgaaa gaactcatcg
     1321 tccgcggcgg cgtcaatatt tccccgttgg aaatcgacga cgtgttgaag ggacacccgg
     1381 ccgtgcagtt cgccatggcc ctcccctttg aaaatcgcta ctacggcgaa gagatcgccg
     1441 cctatatcgt gccgaaagac ccggagtcac cgccgacgga ggccgacatc ctctcccatt
     1501 gccgcggacg gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctggatt
     1621 cctccggcac ccatttcctg gtcaacggcg aaaaactctg gtgtacgaat gggccgattg
     1681 cccggtacct cacgctcgtc gctcgcgtgc cggctcttcg ggtggagtgc gagggaaagg
     1741 tcgaatggat tccggcaccg caggggcagc gggcggacga tcacgttcac acggccttta
     1801 ttttggacat ggcgacacca ggtgtgctgg tgcgccaacg ctgtcagttt gaaggctgtc
     1861 gcgggattga gaacgcccat atcgccttga agaatgtgca ggtaccgatc gaacaggtga
     1921 tcggcgacat cggcaaggga ttgaagtatg ccctgacgat tctgaacgtg gggcggggca
     1981 tcagcattcc cgccatctgc ctgggcatgg ccaaacaggc ctggcaaccg accttggatc
     2041 gagccaatag cagggtcacg tttcagaagc cgttggcaga gcggcagacg cagcagatcc
     2101 ggatcggaga catggccggc cacctgttcg ccatggaggc gctggcgctg ctcgtctggc
     2161 gcctggccga tcagcaccgc tacgacattc gcatcgaagc cgcggtcgcc aagattttct
     2221 gttcggaaca cacgatccgg ttcatccgtg acgcgcagac gattttcgga gggatggggt
     2281 acgaaaccgc cgactccaag cacgcgcgcg gggaagccgc cttcggcatc gaacaactcg
     2341 ttcgcgatgc ggagatgtat cgaatcggcg aaggcgccac cgatatcttg cgccccttcg
     2401 tggtccgcga aggcttgagc cctcatctgg atcgtgccaa gcgcttctat gcagatggtc
     2461 tttccatact cgaacaagcc cggcaggcga tgacgctcat gcgcttctac ctgccctggt
     2521 acctccggca atggcgaaaa cggcctctgc cggaccgtcg agaaatcacc cacccccagg
     2581 tacggccggc ggccctctat gtggaacgaa ccagccggcg gctggcgcga gcaattttct
     2641 acgccttgct gcgattccag gcctcattta aggacgagca gcgtctgcag aacaaaatcg
     2701 aatccgtcgg tgaagatctc cttgccatgc tcgccacggt gctctatgcc gaacgacaga
     2761 ccaggctcga cggacatacc agcgtgtggg aattggccga ggcgttctct gctgcggcga
     2821 aacagcgaat tgaacaacgg ttgagagagt tccggcatca ccgggacacc actgccgcga
     2881 ccaccgggcg gcaagccctc aaagggtact atcccacgct gtcggagggg atcattcgcc
     2941 ggcgcttaga agactatcgt cgcaaccagc acatgacgtg agcgaagagc acgcatcgcc
     3001 cggtgagttc gagagacgt
//
LOCUS       DAOQRM010000241         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_38699_length_2961_cov_19.358224, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000241 DAOQRM010000000
VERSION     DAOQRM010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38699_length_2961_cov_19.358224"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PKK81_12890"
     CDS             <1..720
                     /locus_tag="PKK81_12890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV33830.1"
                     /translation="GEMSAGMAHEIRNPLGALAGAMQMLRQDVGSDDTSQRLMDIAIR
                     EARRLDNIITEFLQYARPPALNLAEHDLNKVLADTLDLIQHEARSRTGITIVSRMAPN
                     QLMAQVDQDQLKQVFWNLATNAFDAMSAGGTLTIATGVRRVEVGGRRSDVIEIAFQDN
                     GEGIPKQNFDKIFLPFFTTKKEGSGLGLAQVHRIVELHEGWIKVESEVGHGARFVVCL
                     PQSAETGVRLRHEGREPWKRF"
     gene            705..2084
                     /locus_tag="PKK81_12895"
     CDS             705..2084
                     /locus_tag="PKK81_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV33831.1"
                     /translation="MEKILVVDDEQGLRDVLSIMLKRAGYAVTVASDGDEAIAQVQKE
                     IFDLVITDLKMPRVGGLEVLKAVKAASPDTVVLMITAFASADSAVEAMKHGAYDYLTK
                     PFQVDEVQLIIRNAIERRRLSTENMLLKRELASQSSFSRIIGQSEAMQKVYDVIKKVA
                     DSKSNVLIGGESGTGKELVARAIHFNSARASMPFVTVNCSAVPETLLESELFGHMKGS
                     FTGAISNKAGLFEVANGGTIFLDEIGDTTPAIQVKLLRVIQEREFRRVGGTQDVKVDV
                     RIVAATNRDLEKAVAEGAFREDLYYRLDVIPIKLPPLRMRTGDIPLLSQHFLEKFAKE
                     SGKPVPTMSQEAMRVLLAHEWRGNVRELENVVERVVAFTTGSSVTDADIRGWLHKPVS
                     NQQALPSELPEDGLDLEGLINTIEKDLLLKALERSQWVKKRAARLLRLNTRSFRYRLE
                     KYEIKGGRD"
     gene            2105..>2961
                     /gene="ispE"
                     /locus_tag="PKK81_12900"
     CDS             2105..>2961
                     /gene="ispE"
                     /locus_tag="PKK81_12900"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNV33832.1"
                     /translation="MNSTSLRVLTPAKVNLILRVLERRPDGFHAIWSLMHTVGLQDEL
                     TLTVQPGTTSRIALRCDHVALAADQSNLVYRAAQLVLARIDRPVDLSISLTKRIPLGA
                     GLGGGSSDAAATILGLTRLLGLNWPIEQMAELGQQLGSDVPFFFMGPAACVTGRGEQV
                     RSMQMTGTRWIVLVNPGFPVETKWAYQQLSATRVGVRPLSPALQQLGSRAAVDWSEII
                     PLVENDFEAPVFSQHPVLAQIKSQLLSQGAEVALLSGSGATMFGVFPGQADAERAASV
                     FALDPKMKA"
BASE COUNT          605 a          797 c          965 g          594 t
ORIGIN      
        1 ggtgaaatgt cggcgggcat ggcgcatgaa atccgcaatc cgctcggcgc gcttgcgggg
       61 gcgatgcaga tgctgcggca ggatgtcggg tcagacgaca ccagtcagcg cttgatggat
      121 atcgcgattc gggaagccag gcggctcgac aatatcatca ccgagtttct gcaatatgcc
      181 cgcccccccg cgttgaattt ggccgagcac gatttgaaca aagtccttgc cgatacattg
      241 gatctgatcc aacatgaagc gcggtctcga accggcatca ccatcgtctc ccgcatggcg
      301 ccgaaccaac tcatggcaca ggtcgatcaa gaccagctga agcaggtctt ctggaattta
      361 gccaccaacg ccttcgatgc gatgtccgcc ggagggacgc tgacgatcgc caccggagtg
      421 cggcgggtgg aggtcggtgg ccgccgatcc gacgtcatcg aaatcgcatt ccaggataac
      481 ggggaaggca ttcccaaaca gaatttcgac aagatctttt tgccgttttt tacgacgaaa
      541 aaagaaggct ccgggttggg gctggcccag gttcatcgca ttgtggagtt gcacgagggg
      601 tggatcaagg tcgagagcga ggtcgggcac ggggcacggt tcgtcgtgtg tctcccgcag
      661 tccgcagaga cgggtgtccg gctccggcat gaaggaaggg aaccgtggaa aagattttag
      721 tcgtcgatga tgagcagggc ttgcgcgatg tgttgagcat catgctcaag cgcgccggat
      781 atgccgtgac cgtggcctcg gacggggatg aagcgatcgc gcaagtgcag aaggaaattt
      841 tcgatctcgt gatcaccgac ctcaagatgc ccagagtggg cgggctcgag gtgctgaagg
      901 ccgtcaaggc tgcgtctccg gacaccgtgg tgctgatgat cacggccttt gcgtccgccg
      961 actcggcggt cgaggcgatg aagcacggcg cctacgacta tctcaccaag ccgttccagg
     1021 tcgatgaagt gcaactgatt atccgcaacg cgatcgagcg gcgccgatta tcgacggaga
     1081 atatgctcct gaagcgggag ctggccagcc agtcgtcgtt ctctcggatc atcggtcaga
     1141 gcgaggcgat gcagaaggtc tatgacgtca tcaagaaggt ggccgattcg aaaagcaatg
     1201 tgttgatcgg cggggaaagc gggaccggca aagagttggt cgctcgggcg atccacttca
     1261 acagtgcgcg cgcctccatg ccgttcgtga cggtgaactg cagcgcggtg ccggaaactc
     1321 tgcttgaaag tgaattgttc ggccacatga agggttcgtt caccggggcg atctccaata
     1381 aagccggtct gttcgaggtg gcgaacggcg ggacgatctt cctcgatgag atcggcgata
     1441 cgacaccggc catccaggtg aagctgctgc gggtgattca ggagcgtgag ttccggcggg
     1501 tgggcggaac ccaggatgtg aaggtggatg tccgcatcgt cgcggccacg aaccgtgatt
     1561 tggagaaagc cgtcgcagag ggcgccttcc gggaagatct gtactaccgg ctggatgtaa
     1621 tcccgatcaa gcttcccccg ttgcgcatgc ggaccggcga cattccgttg ttgagccaac
     1681 atttcctgga gaaatttgcc aaggaaagtg gtaaaccagt gccgacgatg agccaggagg
     1741 ccatgcgcgt gctgttggcg catgagtggc gaggcaacgt tcgggaattg gagaacgtcg
     1801 tcgaacgtgt cgtggccttc acaacgggat cgtcggtgac ggatgcggat attcgcggat
     1861 ggctccacaa gccggtgagc aatcagcagg ccctgccgtc tgagttgcct gaagatggcc
     1921 tggacctgga agggctcatc aacacgatcg agaaggatct gctgttgaaa gcgctcgaac
     1981 ggagtcagtg ggtcaagaag cgagccgcgc ggttgttgcg gttgaatacc cggtcgttcc
     2041 ggtaccgtct cgaaaaatac gaaatcaaag gaggccggga ttagcgccgc ccagtaatct
     2101 gcgagtgaac tctaccagcc tgcgtgttct gacccctgcg aaagtgaatc tgatcctccg
     2161 cgtgcttgaa cggcgtcctg acggctttca cgccatctgg tcgctgatgc acacggtcgg
     2221 actccaagac gaactgactc ttaccgtcca gcccggcacg acctcccgta tcgccttgcg
     2281 ctgcgaccat gtcgcactgg cggccgatca gagcaatctt gtctatcgag ccgcgcagct
     2341 cgtgttggcg cgaatcgatc gacccgtcga tctgtcgatc agcctgacca aacgcatccc
     2401 gctgggggcc ggcttggggg gcggcagcag cgacgcggca gcgaccatcc tcggtctcac
     2461 ccgcctgttg ggattgaact ggccgattga gcagatggcg gagttgggac aacagctggg
     2521 aagtgacgtg ccgttctttt ttatggggcc cgccgcctgc gtcaccggtc gaggtgaaca
     2581 ggtgcgatca atgcagatga cgggcactcg ctggatcgtg ctggtgaatc ccggctttcc
     2641 tgtcgaaaca aagtgggcct atcagcagct gtctgccacc agggtggggg tgcgtcctct
     2701 ctctccggca ttgcagcaac tgggcagccg tgctgcagtg gattggtccg agatcattcc
     2761 cctggtagag aatgattttg aggcgccggt tttttcgcag catccggtat tggcccagat
     2821 taagagccaa ttgctgagtc agggagctga agtggccctg ttgtccggga gcggtgctac
     2881 gatgttcggg gtatttcccg ggcaggccga tgcggagcgg gctgcctctg tgtttgccct
     2941 tgacccgaag atgaaggcct a
//
LOCUS       DAOQRM010000242         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_39723_length_2911_cov_16.849790, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000242 DAOQRM010000000
VERSION     DAOQRM010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39723_length_2911_cov_16.849790"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PKK81_12905"
     CDS             complement(<1..754)
                     /locus_tag="PKK81_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNV33833.1"
                     /translation="MKSCVYEGTVRHRRDAPARHSFHYSIFMMYLDLAELPDLFHERW
                     FWSPATPNLAWFRQSDHLGDPEKPLDQNVRDLVESKTGRRPAGPIRLLTHLRYFGYGF
                     NPVSFYFCFDPADRLVETVVAEVTNTPWGERHCYVLGASLDEGIGRRHRYRFGKQFHV
                     SPFMGMDVDYDWRFGTPGAALSVHMENFRGGERFFDATMVLRREEMTGRSLARVLLRY
                     PLMTTQVIAAIHWQALRLWLKRCPVIPHPDRSR"
     gene            complement(751..>1468)
                     /locus_tag="PKK81_12910"
     CDS             complement(751..>1468)
                     /locus_tag="PKK81_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV33834.1"
                     /translation="RYLVQFFKNHGMLSVDARPTWRVVTGGSRQYVECLVRPFRDRIR
                     LQSPVESVTRYADRVEVRGCDPRGIHRTESFDGVLLACHSDQALALLADPSPLEKEIL
                     GAIRYQPNEAVLHIDRSLLPRRRLAWAAWNYHLVPNPPDHALVTYHMNRLQGLSAPYD
                     FCVTLNHSAAIDPATILQRITYHHPVFSPEAIAAQQRHGEINGRRRTWYCGAYWGFGF
                     HEDGVKSAMTACQALTAGHA"
     assembly_gap    1469..1568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1585..2355)
                     /locus_tag="PKK81_12915"
     CDS             complement(1585..2355)
                     /locus_tag="PKK81_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV33835.1"
                     /translation="MSRTSLNARRVWVTGASTGIGRAVTLELVRRGATVFASARNESA
                     LNELVRDCGGDRVTALPFDVTDRAANQRAVNDMHRRAGGLDIAVLNAGTCEYVDVAAF
                     DSALFERTIRTNFLSMVYGIEAALPLLRQSPAPQLVGMSSTVAYRGLPRSEAYGASKA
                     AIKNLFESLQVDLAPEGISVSVVCPGFVRTPLTDRNDFPMPFRIEAEDAARRIVDGIE
                     ARKPEIHFPRRFSLLFKCLALLPSRWYIGLCARMVKQS"
     gene            complement(2359..2775)
                     /locus_tag="PKK81_12920"
     CDS             complement(2359..2775)
                     /locus_tag="PKK81_12920"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNV33836.1"
                     /translation="MQPVVERFKQIYSGLSVRSLGLLNELYSDDVEFQDPFHRIAGLP
                     ALRQYLAALYAQVEACSFRFEDDVRQGNCAVLTWTMQLNHPRLNAGAAVTVPGSTLIR
                     FRDKVFYHRDYFDAGAMVYEQLPLIGMLIRTIKGRV"
BASE COUNT          581 a          903 c          839 g          488 t
ORIGIN      
        1 gcctggacct gtcgggatgg ggaatgacgg gacaacgttt caaccacagg cgcaacgcct
       61 gccaatggat cgccgcaatc acctgcgtcg tcatcagcgg gtaccgcagc aacacacggg
      121 ccagggacct accggtcatc tcctcccggc gcaggaccat cgtggcatcg aagaaccgtt
      181 ctccaccgcg gaaattctcc atgtgcacgg aaagagcggc accgggcgtg ccgaaccgcc
      241 aatcgtagtc cacatccatg cccatgaacg gagagacgtg aaattgcttg ccgaagcgat
      301 accggtgtcg acgaccgatc ccctcatcca gggaagcacc gagcacataa caatgccgtt
      361 cgccccacgg tgtgttcgtc acttcagcca caacggtctc aaccagccga tcggcggggt
      421 cgaaacaaaa ataaaagctc accgggttga agccgtaccc gaagtaccgc aggtgcgtga
      481 gcagccggat cggaccggcc ggccggcgac cggtcttcga ttcgacgaga tcacgcacat
      541 tctgatccag aggcttctcc ggatcgccca gatggtcgct ctgcctgaac caggccaggt
      601 tgggggtcgc gggagaccag aaccagcgtt catggaagag gtccggtaat tcggccagat
      661 ccagatacat catgaagatc gagtagtgga acgaatggcg ggccggcgca tcgcgtcgat
      721 gcctgaccgt tccttcatac acgcagctct tcatgcatgc cccgccgtga gcgcctgaca
      781 ggccgtcatg gcgctcttca cgccgtcttc atggaaaccg aagccccagt aggccccgca
      841 ataccacgtc cggcgacgtc cgttgatctc cccatgccgt tgctgcgcgg cgatcgcttc
      901 gggggaaaag accggatggt gatacgtgat gcgctggagg atcgtcgccg gatcgatcgc
      961 cgcgctgtga ttcaacgtca cgcagaaatc gtacggcgcg ctcagcccct ggagtcgatt
     1021 catgtgatag gtcaccaagg catggtccgg cgggttcggc accagatgat agttccaggc
     1081 cgcccaggcc aggcggcgtc tcggaagaag cgatcggtct atgtgcaaca cggcttcatt
     1141 cggttgataa cggatcgccc cgagaatttc cttctccagg ggagacgggt ccgctaacag
     1201 cgccaacgcc tggtcgctat ggcaggcgag aaggactccg tcgaatgact cggtccggtg
     1261 gatcccgcga gggtcgcatc cccgcacctc gacacggtcc gcgtaacgag tgaccgattc
     1321 caccggagac tgaagccgga tgcggtcacg gaagggccgc accaggcact cgacatattg
     1381 ccgggagccg ccggtcacca ccctccaggt cggacgcgca tcgaccgaca acatgccgtg
     1441 gttcttgaaa aactgaacga ggtaccgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnncc gacaatggcg agcttcatga ctgcttcacc atcctggcgc agaggccgat
     1621 gtaccagcgg ctcgggagca gggccaggca cttgaagagc aggctgaatc gccttgggaa
     1681 atgaatctcc ggcttgcggg cctcgatccc gtcgacgatg cgtcgggccg cgtcctcggc
     1741 ttcgatcctg aacggcatcg ggaaatcgtt ccggtcggtc aacggtgtcc gtacgaaccc
     1801 cggacacacg accgacacgc tgataccttc gggcgcgaga tcaacttgca gtgattcgaa
     1861 gaggttcttg atcgccgcct tcgacgcccc gtaggcttcg gagcgaggca acccgcgata
     1921 ggccaccgta ctgctcatcc ccacgagctg cggcgccggt gactgccgca gcagcgggag
     1981 cgccgcttca atcccataaa ccatgctcag gaagttggtg cggatcgtcc gctcgaacag
     2041 cgcactgtcg aatgccgcca catccacgta ctcacaggtg ccggcgttga gaacggcaat
     2101 gtccaggccc cctgcacggc gatgcatgtc gttcaccgcg cgctgattgg cggcccggtc
     2161 ggtcacatcg aagggaagcg ccgtgacacg atcgcctccg cagtcacgga cgagctcgtt
     2221 gagcgccgat tcgttccgcg ccgaggcaaa gacggtggcc ccgcgacgaa cgagttccag
     2281 cgtgaccgcc cggccgatac cggtcgaggc gccggtcacc cacacccgtc gagcattgag
     2341 actggttcgg ctcatgggtc acactcggcc tttgatcgtc cggatgagca tgccgatcag
     2401 cgggagctgc tcgtacacca tcgcaccggc atcgaagtag tcgcggtggt agaacacctt
     2461 gtcccgaaaa cgaatgagcg ttgaaccggg aaccgtcacc gccgcgccgg cattgagcct
     2521 cgggtgattc agctgcatgg tccaggtcaa tacggcacaa ttgccttgcc ggacatcgtc
     2581 ctcgaaccgg aatgaacagg cctccacctg cgcgtagagc gcagccaaat attggcgcag
     2641 ggcggggagt ccggcgatcc ggtgaaaggg gtcttgaaac tcgacgtcgt cgctgtacaa
     2701 ctcgttcagc agtcccaacg agcgaaccga cagtccgctg tagatctgtt tgaatcgttc
     2761 gacaaccggt tgcacgacag cggactcctc tcctgctctt ctacgaagga acctgaggga
     2821 cggattcttt cagcgccccc catcatcacc ggcgctagcc cccctcccgc cggtgcatga
     2881 gcccctcttt caacgcttca ttgatcggat c
//
LOCUS       DAOQRM010000243         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_41626_length_2828_cov_14.477822, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000243 DAOQRM010000000
VERSION     DAOQRM010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41626_length_2828_cov_14.477822"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /gene="pyrH"
                     /locus_tag="PKK81_12925"
     CDS             <1..225
                     /gene="pyrH"
                     /locus_tag="PKK81_12925"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNV33837.1"
                     /translation="YDSDPVKNPQAKKYAEIPFLSILTQNLKVMDSTAISLCMDNRLP
                     LIVFNLKEKGNFKRVVQGEPIGTLVTVESR"
     gene            237..800
                     /gene="frr"
                     /locus_tag="PKK81_12930"
     CDS             237..800
                     /gene="frr"
                     /locus_tag="PKK81_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNV33838.1"
                     /translation="MSTAQEVKQRVTEKMDHAIEHLKRDLAGLRTGRASVALVDGIKV
                     DYYGTMTPLKQVANVATPEARLITIQPWEQNLIREIEKAIQTSDLGLTPSNDGKLIRI
                     PLPPLTEERRKELIKVCKKHGEEVKVQIRGFRRDGNEELKKLQKDTKLTEDELRKSEA
                     EIQKLTDQYVQKIDDVMKKKEGEILEV"
     gene            827..1990
                     /gene="alr"
                     /locus_tag="PKK81_12935"
     CDS             827..1990
                     /gene="alr"
                     /locus_tag="PKK81_12935"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV33839.1"
                     /translation="MYTPSQFPPTSVSVDLNALAQNLAHVRRLAPRSEVLAIVKANAY
                     GHGALELTRTLQQLAVHRFGVATVDEGIALRQAGIHDAILVMGPTVPAQFPDLIAHRL
                     TPVLYRSDMVQVFAATISPNAAPYSVHVKIETGMGRLGVLPQEVPDLVAMPAFRATLR
                     LEGLMTHLADADNTETTHTEKQLAEFQQTLDTLRQRGISCPLTHAANSAGIIKYPASL
                     YSLVRPGIMLYGYHTLSNAAAAPELKPILTWKATIAHLHNIQPGDSVSYNRTFVASRR
                     SRIAVLPMGYADGYNRLLSNRGVVLIGGRRVPVVGRVCMDMTMVDVTDVPGVQVGHEA
                     ILIGQQGQERITAADLAAWQQTIPYEVLCAIGQRVPRHYLPLLSADKTSSPKK"
     gene            2092..>2828
                     /locus_tag="PKK81_12940"
     CDS             2092..>2828
                     /locus_tag="PKK81_12940"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNV33840.1"
                     /translation="MGGRRRFDRNSLVCFSAAILSLCATAPAAAAGTGTARHQQMEAS
                     SMTIHVPIPMTALAEQYASIHQSLAAHRHTHRDWVTLGGGTGFLKYRIWPGEQSSTTT
                     EGRLLSQSSFPFGVEYAKQIKGTVTKIADCGQQDATTGTGRLSVTLATTFSQARAYTL
                     LPISSVTAVEANRPCLLSEQGVDTAPLMAQVYRSELQGMLPTVDRKAATLVTLKPAVT
                     HIWNDLQEPLLLDEAEGLWLQLNPEAIG"
BASE COUNT          659 a          887 c          767 g          515 t
ORIGIN      
        1 tacgacagcg acccggtcaa gaatccacaa gccaagaagt atgcagagat tccgttcttg
       61 tcgattctca cccagaacct caaggtgatg gactcgacgg ccatcagctt gtgcatggac
      121 aaccggttgc cattgatcgt cttcaatttg aaagagaagg gaaactttaa acgtgtcgtc
      181 cagggcgaac ctatcgggac cctggtcacc gtagaaagtc gctaagggag aggctgatgt
      241 cgacggcgca agaagtgaaa caacgcgtaa ccgaaaagat ggaccacgcg atcgaacatt
      301 tgaaacgcga cctggccgga ctgcgtaccg gtcgcgcatc cgtggcactg gtggacggca
      361 ttaaagtcga ctactacgga accatgacac cgctgaagca ggttgccaac gtcgccaccc
      421 ccgaagcccg gctcattacc atccagccct gggaacagaa cctcatcaga gaaatcgaga
      481 aggcgatcca aacctcggac ctcggcttga caccctccaa tgatggcaaa ctcatccgca
      541 tcccacttcc accgctcact gaagagcgcc ggaaagaact tattaaggtc tgcaaaaaac
      601 acggcgaaga agtgaaggtg cagattcgcg gcttccgccg ggatggcaac gaagaactca
      661 agaagctgca gaaagacacg aagctgaccg aagatgaact gcgcaagtcg gaagccgaaa
      721 ttcaaaaact caccgatcaa tacgtgcaga aaatcgacga cgtgatgaag aaaaaagaag
      781 gcgagatcct ggaagtctag cgccctattc tgacgccaca agctgcgtgt acacgccgtc
      841 tcagttcccc ccaacctccg tctcagtcga tctgaatgct ctcgcgcaga atctcgctca
      901 cgtgcgtcgt cttgcgccgc gttccgaggt actggctatc gtcaaggcga atgcctatgg
      961 ccatggcgcc ctcgaactca cacgcacgct gcaacaacta gccgtccatc ggttcggtgt
     1021 tgctacagtg gatgagggca tcgcgcttcg ccaggcgggc attcatgatg ccattttggt
     1081 catgggcccg acggtaccgg cacaatttcc agacctgatc gcgcatcgtc tgactcccgt
     1141 gctgtaccgg tccgatatgg tccaagtttt tgccgcaacc atttcgccca atgccgcgcc
     1201 ctactccgtg catgtcaaaa tcgagaccgg catgggtcgc ttgggcgtcc tccctcaaga
     1261 ggtgcccgac ctcgtcgcca tgccggcctt tcgcgccacg ctacggctcg aaggcctcat
     1321 gactcatctg gctgatgcgg acaataccga gaccacacac acggagaaac aactcgcgga
     1381 gtttcagcag acactcgaca cgttgcgcca acgtgggatc tcctgtcccc tgacacacgc
     1441 cgccaacagc gccgggatca tcaagtatcc cgcaagcctc tattccctcg tgcgacccgg
     1501 aatcatgctg tatggctacc ataccctgtc taacgcagcc gccgcgcctg aattgaaacc
     1561 gatcctcacc tggaaagcga ccatcgcaca cctgcacaac atccagccgg gcgacagcgt
     1621 cagctacaac cggaccttcg tcgcctcgcg gcgatcgcgg atcgcggtcc tcccgatggg
     1681 ctatgcggac ggctacaatc gcctcctgtc gaaccgaggc gtggtcttga tcggcggacg
     1741 aagggtgccg gtggtcgggc gtgtctgcat ggacatgacg atggtcgatg tgacagacgt
     1801 gcccggcgtg caagtcggcc atgaagccat cctcatcgga cagcagggac aagagcgcat
     1861 caccgcagcc gatctcgccg cctggcagca gaccattccc tatgaagtgc tctgcgccat
     1921 cggccagcgt gtgcctcgcc actatctccc gctgctatca gccgacaaaa cgtcctcacc
     1981 gaagaagtga cggcttgcct tccgtttttg cttcccccat agtcgaccga tatgttcaaa
     2041 gtaagttcgg gtggcggcca ccctctgtgc caccagcatg aggataagac gatgggcgga
     2101 cgacgccggt tcgatcgaaa tagcctggtt tgcttctctg cggccattct gagtctctgc
     2161 gccacagcac ccgcggcagc ggcaggaacc ggcacggccc ggcaccagca gatggaagcc
     2221 tccagcatga cgattcatgt gccgattccc atgaccgccc tggccgagca gtatgcgagc
     2281 attcaccagt cactcgccgc acatcgacac acacaccgcg actgggtaac gctgggcgga
     2341 ggtaccggat ttttgaagta ccggatctgg ccgggagagc agagttcgac gacgaccgaa
     2401 ggtcgcctgc tgtcccaatc cagctttcca ttcggcgtgg aatacgcgaa gcaaatcaaa
     2461 ggcacggtga cgaaaatcgc cgactgcggc caacaggatg ccacaaccgg cacaggccgg
     2521 ttatcggtga cgctggcaac caccttcagc caggcccgcg cctataccct attgcccatc
     2581 agtagcgtta ccgcggtgga agcgaaccga ccctgcctgc tgtccgaaca aggcgtcgat
     2641 acggcacccc tgatggcgca ggtctatcga agtgagttgc aagggatgct gccgacggtt
     2701 gatcgtaagg ctgccaccct cgtgacgctg aagcccgccg tcactcacat ctggaacgat
     2761 ctgcaggaac ccttgctgtt ggatgaagcg gaaggcctct ggctccaact gaatcctgaa
     2821 gccatcgg
//
LOCUS       DAOQRM010000244         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_42120_length_2806_cov_10.818248, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000244 DAOQRM010000000
VERSION     DAOQRM010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42120_length_2806_cov_10.818248"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PKK81_12945"
     CDS             complement(<1..880)
                     /locus_tag="PKK81_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNV33841.1"
                     /translation="MTPQQYAAAVDTTSEVLCLACAGSGKSRTLAYRIARLLAQNEPP
                     EGIVAFTFTEKAAESIKRRVSQALSTAGLDPTMMGAMYIGTIHSYCQHVLGDMDATYR
                     QYDVLDENRLKLYLISRYYQLGLQAFRPRARNNGYFDTIKQASDAWKTANDELLDFNA
                     VTAEDRDIGDLLSRIRDGLRTDQYIDFSLMIRNVVDGIRANAASVENAIGSLRHLMVD
                     EYQDVNPCQEELIRLLHQRSQTLFVVGDDDQSIYAWRGADVSNILGFQRRYAGCSVHT
                     LSQNFRSTEPIVQASDA"
     gene            complement(937..1971)
                     /locus_tag="PKK81_12950"
     CDS             complement(937..1971)
                     /locus_tag="PKK81_12950"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNV33842.1"
                     /translation="MDGKPTAVDLFCGCGGLTVGLKRAGFRVMGALDIDPLSIKTYKA
                     NHRDVTLWETDIRELDPQELLDTLGLKKGGLDLLAGCPPCQGFSTMRTLNGALRVDDP
                     RNDLLMEFERFVDALRPRAVMLENVPGLADDKRFETFLRKLKKLGYLGAHRVLNAAEY
                     GVPQRRRRLIYLAGLGMVIPFAEKNGRKKTVKEAIGRLPKAGQSGDPVHDMPENRTPK
                     VLEIIRLIPKDGGSRRDLPEGFQLECHKRCSGFKDVYGRMAWKDVAPTITSGCFNPSK
                     GRFLHPEENRAITMREAALLQGFPRWYKFPITDNKSAVALMIGNALPPPFIEAHGRSI
                     KRVLQGTAGT"
     gene            complement(2181..>2806)
                     /locus_tag="PKK81_12955"
     CDS             complement(2181..>2806)
                     /locus_tag="PKK81_12955"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNV33843.1"
                     /translation="IDLADDTERSVGVPEQGGAGVISQDPLPPGTVYTAAVDSEAKVA
                     LFRLEVTLTAGTGKLRTPTGLEKSLKESLNRAFSYLQNVKEKLGLTQMLAQKDIYAEA
                     VDLSGGRIDCPCGVAFFAAIISAVRNRHVQAGTVILGDLTIQGNIKGPASITEPLQLA
                     LESGALRILIPVSNKTQFAGLPEDVIERLDVVFYGDVDRAVLKTLEM"
BASE COUNT          555 a          841 c          775 g          635 t
ORIGIN      
        1 aggcgtccga agcctggacg atgggctccg tgctgcggaa attttgggag agggtatgca
       61 cgctgcaacc ggcatagcgt cgttgaaaac ctaagatgtt gctgacatcg gccccccgcc
      121 atgcatagat cgactggtcg tcgtctccaa ccacgaaaag cgtctgcgag cgctgatgca
      181 gcagtcggat cagctcctcc tggcaggggt tgacgtcctg atattcatct accatgaggt
      241 ggcgcaaact cccaatggcg ttttccacgc tagctgcatt ggcgcggatc ccgtcaacca
      301 catttcggat catcaaggag aaatcgatat attgatccgt cctcagtccg tccctgatac
      361 gagaaagcag gtcaccgatg tctcgatctt cggcggtgac cgcgttgaag tccaacaatt
      421 cgtcgttggc cgtcttccac gcgtccgatg cctgtttgat agtgtcgaaa tagccgttgt
      481 tacgagctcg cggcctgaag gcctgaagcc ccaactggta gtaacgtgaa atcaggtaga
      541 gcttcagtcg attctcgtcg agcacgtcgt actgtcggta cgtggcgtcc atgtcaccca
      601 gcacgtgctg gcagtaggag tggatggttc cgatatacat cgcacccatc atcgtggggt
      661 cgagtcctgc ggtcgaaagg gcttgagaaa ctcggcgttt gatggactca gccgccttct
      721 cggtaaacgt gaaggccacg atcccttcgg gcggctcatt ctgagcgagg agcctggcga
      781 tccggtaggc caatgtcctc gacttgcccg agccggcgca tgccagacac aatacttcgc
      841 ttgtggtatc caccgccgct gcgtattgct gcggcgtcaa ctcgttcctc aagacctggt
      901 cgatactcat gtgaatttgg tccctctcct gaatggttac gtcccggcgg tgccctgcag
      961 gactcgtttg atgcttctgc cgtgtgcctc gatgaacggc ggcggcagtg cgttgccgat
     1021 catcaaagcc acggcggact tgttgtctgt gataggaaac ttgtaccaac gggggaaccc
     1081 ttggagaagc gccgcctcgc gcatggtgat cgcacgattc tcctccggat gaagaaatct
     1141 ccccttggaa gggttgaagc acccgctggt gatcgtcgga gccacatcct tccaagccat
     1201 gcgaccgtaa acatctttga aaccgctgca tcgcttgtgg cactcaagtt ggaatccctc
     1261 aggcagatct ctgcggcttc cgccgtcttt cgggataagc cggatgatct ccaggacttt
     1321 gggagttcga ttctcgggca tgtcatgcac ggggtccccg ctttgtcccg ctttcggcag
     1381 gcggccgatg gcttccttga ccgttttttt tctgccgttc ttttcggcga acgggatgac
     1441 catgcccaac cccgccagat agatcaacct gcgtctccgc tgcggcacgc cgtactccgc
     1501 cgcattcaga acgcggtggg cgccgagata gccgagtttt ttcaacttcc ggagaaaagt
     1561 ctcgaaccgc ttgtcgtccg ccagccccgg gacgttttcc aacatcacgg cacgcgggcg
     1621 gagagcatcc acaaaccgct cgaactccat cagaaggtcg tttcgagggt catcgaccct
     1681 taacgctccg ttaagagtcc gcatcgtcga aaatccctgg cacggcggac acccagccaa
     1741 gagatcgagt ccacctttct tcaaccccag cgtgtcgagc agttcctgcg gatcaagttc
     1801 tctgatgtcc gtttcccata atgtgacatc gcgatggttc gctttgtagg tcttgatgga
     1861 cagcggatcg atgtccagcg cccccatgac ccggaatccg gctctcttca accctacggt
     1921 caatccaccg cacccacaga aaagatcaac ggccgtgggc ttgccatcca tgccacggga
     1981 cggtcctcgt tttatccttg aatcactcct cattgtaagc acctcctgtt atctaaatac
     2041 gtgcttccta caagcacttt caggaggtta cttaccgacg gaccgactga accgccggct
     2101 gaatccgtgg aacatgagcg gcttatccca gagggtttgc atgtaatttt ccgagtatcc
     2161 atcgctccca gccgcatccg ttacatctcc agtgtcttga gtacggcccg gtcgacgtca
     2221 ccgtaaaaca ccacgtcgag gcgctcgatc acatcttccg gcagtccggc gaactgagtc
     2281 ttattggaaa cagggatcag gatacgcaaa gccccggatt cgagtgccag ttgcaggggt
     2341 tcggtgattg aagccgggcc cttgatattc ccttggatgg tcaggtcgcc gagaatcacc
     2401 gtaccggcct gcacatgacg attgcgcacc gcggagataa tggcggcgaa aaacgccacg
     2461 ccgcaggggc agtcgatgcg gccgccggag aggtccacgg cttcggcata gatatccttc
     2521 tgcgccagca tctgggtaag cccgagtttt tccttgacgt tctgcaggta gctgaatgct
     2581 ctgttcagcg attctttcag cgatttttca agtcccgtcg gggtccggag cttgcccgtg
     2641 cccgccgtca gggtcacctc gagcctgaac aaggcgactt tggcttcgga atccaccgca
     2701 gccgtgtaga ctgttccggg aggcagcggg tcctgcgaaa taactccggc gcccccttgc
     2761 tcgggcacgc ccacggaccg ctcggtgtcg tccgcaagat cgatgt
//
LOCUS       DAOQRM010000245         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_43359_length_2753_cov_17.057079, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000245 DAOQRM010000000
VERSION     DAOQRM010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43359_length_2753_cov_17.057079"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /gene="thiC"
                     /locus_tag="PKK81_12960"
     CDS             complement(<1..1585)
                     /gene="thiC"
                     /locus_tag="PKK81_12960"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNV33844.1"
                     /translation="MSEAHTQNGSSNGHGSKPSTARLTTTPFAASRKVHVDGALPGVR
                     VPMREISLTPTQSANGQGPTPNQPITIYDTSGPYTDPNVTIDVRAGLPPLRRAWIESR
                     RDTEELSQVTSQYGRQRAADPKLADLRFTHIRKPLRAKAGHNVSQMHYAKKGIITPEM
                     EFIAIRENQSRARAKELLASTNGHGGGVLQHPGQAWGARIPNIITPEFVRDEVARGRA
                     IIPNNINHPETEPMIIGRNFLVKINSNIGNSAVASSIEEEVEKMIWSIRWGADTVMDL
                     STGKNIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTAKRMTGIVSRGGSIHAKWCLSHHQENFAYTHFEEICEIMKAY
                     DVAFSLGDGLRPGSIADANDEAQFAELETLGELTKIAWNHDVQVMIEGPGHVPMHMIQ
                     ENMEKQLKECQEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHL
                     GLPTKEDVKVGVVTYKIAAH"
     regulatory      complement(1677..1780)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2357..2656
                     /locus_tag="PKK81_12965"
     CDS             2357..2656
                     /locus_tag="PKK81_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33845.1"
                     /translation="MLLHIALQEGFHHDEVRVSVDAREVWGSADLTTRRQTGYAAAVE
                     VDVAHTPVVVEVVLSKRGVAQTKSVSVDRPTYLGVSLTTEGNLAWKTSYEPFGYL"
BASE COUNT          531 a          766 c          853 g          603 t
ORIGIN      
        1 catgggcggc gatcttgtac gtcacgacac cgaccttcac gtcttccttg gtcggcagac
       61 cgaggtgttc tttcggcgtg acgtaacaga gcattgcgca accataccag ccgatcatcg
      121 cggcaccgat gccggaggta atgtggtcat aaccgggagc aatgtccgtc gtgagcggcc
      181 ccagcgtgta gaacggcgcc tcctggcact ccttcagctg cttctccatg ttctcttgaa
      241 tcatatgcat cggcacatgg ccgggacctt cgatcatcac ctggacatca tggttccacg
      301 cgatcttggt gagttcgccc aaggtctcca attcggcaaa ttgcgcctcg tcgttggcat
      361 cggcaatcga accgggccgt aagccatcgc ccaggctgaa ggccacatcg taggccttca
      421 tgatttcgca gatctcttcg aagtgcgtat aagcgaagtt ttcctggtga tgcgagaggc
      481 accatttggc atggatcgag ccgccgcgcg agacgatgcc ggtcatgcgt ttcgccgtca
      541 tcggcacgta acgcagcagg acaccggcgt ggattgtgaa gtagtccaca ccctgttcgg
      601 cctgctcgat cagcgtatcc cggaaaattt cccaggtcag gtcttcggcc ttgccgttga
      661 ccttttcgag cgcctgatag atcggcacgg taccgatcgg tacgggcgag ttgcggatga
      721 tccactcgcg tgtctcgtga atgttcttgc cggtcgagag atccatcacc gtgtcggcgc
      781 cccaacggat cgaccagatc atcttttcga cttcttcctc gatcgaggag gcgaccgcgg
      841 aattgccgat gttggaattg atcttcacga ggaagttccg gccgatgatc atcggctcgg
      901 tttccgggtg gttgatgttg ttcgggataa tcgcgcgtcc gcgggccact tcgtcgcgca
      961 cgaattcggg ggtgatgatg tttggaatac gcgcgcccca ggcttggccg ggatgctgca
     1021 acacgccgcc gccatggccg ttggtcgagg ccaggagttc tttggccctt gcgcgggact
     1081 gattctcccg gatcgcgatg aattccattt ccggcgtgat gatgcctttc ttggcatagt
     1141 gcatctggct gacgttgtgt ccggctttgg cgcggagcgg cttgcgaata tgcgtaaacc
     1201 ggaggtcggc caatttcggg tccgcggctc gctgacgccc gtactgggat gtgacctgcg
     1261 aaagttcttc cgtatcccgg cgactctcga tccaggcccg gcgcaagggc ggcaggccgg
     1321 cgcgcacatc aatcgtcacg ttcggatcgg tataaggacc ggaggtgtcg taaatcgtga
     1381 tcggctggtt gggcgtaggg ccctggccgt tggctgactg cgtgggggtc aggctgattt
     1441 cgcgcatcgg gacccggacc cctgggagcg cgccgtcaac atgcaccttg cgcgaagccg
     1501 caaaaggcgt cgtagtgagg cgtgcggtcg agggcttgga gccatgaccg ttgctggaac
     1561 cgttctgtgt atgggcctcg ctcatagcgg acatcctttc gtcggaggtg agcgtctgac
     1621 agccgaaata aaaaaagccg catccagcga gggtgcggct taaagcgatc acgaagccag
     1681 tcaaccggtc gcttccctac gctggtatta cccaggtcag gttgtgaggg tcgcccgagc
     1741 aagtcggact ctcagccgga tggcacccct agctgtcgaa gatgatcgta ccgacctgcc
     1801 ggagcgaagt caatcttccg gaggacctag tggtcaaggg gcttctcggg cagggaattc
     1861 cagttcgggc gagtaatgcg gagtgcctag gcaagtggcc tgaataatgc ggcgggagat
     1921 tcgtcgcgga atgttcaatg cacaatgggt catgctcaag ggatggttga acgccgggtg
     1981 cattcagggt gagaagttgc acgcttgcgc atcatcggag agtaccaatt gcaagccaga
     2041 gcgaagaggg gccgcgttgc gatgaccgtt tgtatcgcat cagataccgc cctcgtatcg
     2101 tatcggatac gagaacgccc gctggttcct cccaagattc ccgtgaagtc tgcgtcgtgc
     2161 ttccgtgccc gccgccatcc tgctggtcgc cgcaaacatt tcgcgaggtt ccaaatactt
     2221 gtttttatct cctgaccgct gcgtgaccag acgcctcacc cggtataccg tttgcttttg
     2281 acagggagaa cagcagtgta aggggacctc tcccccagtc aatcttgatc actccatagc
     2341 tcggacgagg tggctcgtgt tgctgcacat cgctctacaa gagggctttc atcatgacga
     2401 ggtgcgtgtg tcggtggatg cccgggaggt gtggggcagt gccgatctca cgacgcgtcg
     2461 acaaaccgga tatgcggctg ctgtggaagt cgacgtcgca cataccccgg tcgtcgtgga
     2521 ggtcgtgctg agcaagcggg gggtggctca aaccaaatcg gtttcggtgg atcgtccgac
     2581 ctatctcggt gtgtcactca caactgaagg gaacctggcc tggaaaacct cgtacgaacc
     2641 cttcgggtac ctgtgacgag acgtaccgaa acagcatgac gcctgaaggt cgcggtctat
     2701 cacaacagga ggtagcgtat gaaagagcaa gaccaaatcg atcaggagta tcg
//
LOCUS       DAOQRM010000246         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_43565_length_2744_cov_18.325028, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000246 DAOQRM010000000
VERSION     DAOQRM010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43565_length_2744_cov_18.325028"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PKK81_12970"
     CDS             complement(<1..1094)
                     /locus_tag="PKK81_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV33846.1"
                     /translation="MESDHSKQDPRPTTRPAPVVLAPVPVVQGQGHAQVPVRVGAGPL
                     PGRKRRIPLLLAAVGLLAILALGLWHWWSNDPVAGHYKTLPVDRGPITSLVTATGAVN
                     PVISVQVGSQVSGKIAKIYADFNSIVREGQVLASIDQKPYQAKVSQAKAALKSAKATL
                     AKARNMLAQRTLELNRMAALREQQFVAQSDLDLSRTNYRDAEAQVDLSQAQVDQAKAT
                     LSSAELDLGYTTIISPVNGTVVSRNVEEGQTVVASFQTPTLFVIAQDLTRMQVIANVS
                     ESDIGGVSEGTSADFRVDAYPREFFHGVVTQVRNAPVSIQNVVTYDVIISVENPELKL
                     KPGMTANITIVTARNEDALRAPNAALRFRMP"
     gene            1356..2735
                     /locus_tag="PKK81_12975"
     CDS             1356..2735
                     /locus_tag="PKK81_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV33847.1"
                     /translation="MTEEWGAVLVVDDDADMRSLLCDVLQGRGHQCTGVGSGDDALQR
                     LSEEDYAVVLTDLRMKGMLGTELLAEVKHRHPDIGVILMTAFGSVETAVEAIRHGASD
                     YLTKPVKTEELVRVVERAIREAALRREVSRLRKEVHKEYSFHQILGKSKPMQAVFDLI
                     RRVADSPTNVLITGESGTGKELVAKAIHYNSDRRDAPFVPVNCAAIPEQLLESELFGH
                     MRGSFTDAKMDKRGLFEEAQKGTLFLDEISELPLMLQAKLLRAIQEREIRRVGATKSI
                     PVDVRIIAATNLNLSEEVKNKRFREDFYYRLNVIELRLPPLRERREDIPILVDAFLKK
                     CADSRGKQVKGLSESALAMLADYAWPGNVRELENVIERAVTLSHGDMIGADDLPVQVQ
                     GSRGDRRILDEAAERTLPLHEVEKEYIVKILEKTGGNKYQAAHVLGIDRKTLYRKLAE
                     IEGKPHSDA"
BASE COUNT          577 a          739 c          904 g          524 t
ORIGIN      
        1 ggcatacgaa atcgtaaggc cgcgttgggg gcgcggagcg catcttcgtt gcgcgccgtc
       61 acgatggtga tgttggccgt catgccgggt ttgagcttca attcggggtt ttcgacggag
      121 atgatcacgt cgtaggtgac cacattctgg atgctgaccg gagcgttccg gacctgcgtc
      181 acgaccccgt gaaaaaactc acgcgggtac gcatccacac gaaagtcggc ggaggtgccc
      241 tcgctgacgc cgccgatatc cgattcgctg acattggcga tgacctgcat gcgggtgagg
      301 tcctgggcga tcacgaagag ggtcggggtt tgaaagctgg cgacgaccgt ctgcccttcc
      361 tccacgttac gggagacgac ggtgccgttg accggcgaaa taatggtggt gtaacccaaa
      421 tccaattccg cagacgacag ggtggccttg gcttggtcga cctgtgcctg agacagatcc
      481 acctgtgctt cggcatcgcg atagttggtt ctcgacagat ccagatccga ctgggcgacg
      541 aattgttgct cccgcaacgc agccatgcga ttgagctcca gtgtcctctg cgcgagcatg
      601 ttgcgggcct tggccaatgt ggctttggcg cttttgagtg cggctttggc ctggctgacc
      661 ttggcttgat agggtttctg gtcgatggag gcgaggacct gaccttcccg tacgatggag
      721 ttaaagtcgg cgtagatttt cgcgatcttt ccggagacct gactgccgac ctgcacggag
      781 atgacggggt tcacggctcc cgtggccgtg accagtgagg tgatcgggcc gcgatcgacg
      841 gggagggtct tatagtgacc ggcaaccggg tcattggacc accaatgcca caatccgaga
      901 gccaggatcg ccagaagccc caccgccgcg agtagaagcg gtatccgtcg ctttctgcca
      961 ggaagggggc cggcgccgac tctgacaggc acctgagcat ggccctgacc ttgcactacc
     1021 ggaacgggag ccagcacgac cggtgccgga cgggtggtcg gccgcggatc ctgtttcgag
     1081 tgatctgact ccatagacga cctcccgtct cgccagggaa gacgcaagag aagtgccgtt
     1141 gcgggaaagg ttcgcaactc gatgaagtgg cgtgctcacc tcacgggctt gacggctcga
     1201 agattggtct gtgagtgatg gtggggcatt tcgcgacagc aggcggcaat gacctgcggc
     1261 aggacgcccc ttgaccgccc gggagtaagc cgattaggct gtgcgcgcac tcgctggaga
     1321 gccggtccga tgaacggagc acgtgaggga gctgtatgac tgaggaatgg ggcgcggtcc
     1381 tggtggtcga cgatgatgcc gacatgcgca gcctgctgtg cgatgtcttg cagggtcgcg
     1441 ggcatcaatg cacgggtgtc ggcagtggtg acgacgcgct tcagcggctg agtgaggaag
     1501 actatgccgt ggtcctgacc gaccttcgca tgaaaggcat gttgggcacg gagttgttgg
     1561 cggaggtgaa acaccggcat cccgatatcg gcgtgatcct gatgacggcc ttcggatctg
     1621 tggagacggc ggtcgaggcg atccggcacg gtgccagcga ctatctgacc aaaccggtga
     1681 aaaccgagga actggtgcga gtggtcgagc gcgcgattcg ggaagcggcg ctccggcgcg
     1741 aagtcagccg gctacggaaa gaggtgcaca aggagtacag ctttcatcag attctcggca
     1801 agagcaaacc gatgcaggcg gtcttcgatc tgattcggcg cgtggccgac agtcccacca
     1861 acgtgttgat cacgggagag agcggaacgg gaaaagaact ggtcgccaag gccattcatt
     1921 acaacagcga ccggcgggat gcgccgtttg tcccggtgaa ctgcgccgcg atcccggaac
     1981 aattgctgga aagtgaactc ttcgggcaca tgcgcggctc gtttacagat gccaaaatgg
     2041 acaagcgcgg actctttgaa gaggcacaaa aaggcacgtt gtttctggat gaaatcagcg
     2101 aacttccgct catgctccag gcaaaactcc tgcgcgcaat tcaggaacgg gaaatccgtc
     2161 gcgtgggggc gaccaaatct attcccgtgg acgtgcggat catcgccgcc acaaacttga
     2221 acctttccga agaagtgaag aataagcgat tccgcgaaga tttttactat cgcctcaatg
     2281 tgatcgagtt gcgccttccc ccgctacggg aacgccggga ggatattccg attctggtgg
     2341 atgccttcct gaagaagtgc gccgacagca ggggcaagca ggtcaagggc ctcagcgaat
     2401 ccgcccttgc catgctggcc gactatgcct ggccggggaa tgtgcgggag ttggagaacg
     2461 tcattgagcg ggctgtcacg ctgagccatg gggacatgat cggcgcggac gatttgccgg
     2521 tgcaggtgca aggctccaga ggcgatcgcc ggattctcga tgaagccgcg gaacgcaccc
     2581 tgccactgca cgaagtggaa aaagagtaca tcgtgaaaat tttggaaaag acgggcggca
     2641 acaagtatca ggccgcgcac gtcctgggta tcgatcggaa gaccctgtat cggaagcttg
     2701 ccgaaatcga aggcaaacca cactcagacg cgtagattca ccgt
//
LOCUS       DAOQRM010000247         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_47378_length_2598_cov_15.457334, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000247 DAOQRM010000000
VERSION     DAOQRM010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47378_length_2598_cov_15.457334"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKK81_12980"
     CDS             <1..443
                     /locus_tag="PKK81_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33848.1"
                     /translation="NMGQDLYNAFPADYGGKATKGLPVLAGVQNLRDVTYAVSLGAGN
                     PGVEAWYVFGKDKYKFELGGGCTGVIAPGLYPLLRSGQINGLIGGLRGAAEYETLIGQ
                     KGKAVAGMDAQSATHLAIIVLVAICNIFYFTLRRQQRRHDGLGS"
     gene            448..>1197
                     /locus_tag="PKK81_12985"
     CDS             448..>1197
                     /locus_tag="PKK81_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33849.1"
                     /translation="MDLSFDVILGAWIATGLTLFILSFLYEDNPLFKLAEHLYVGVSL
                     GYTIVKTYDTVVMTLILRPILDKGEWSLLIPVAIGMLMLTRYVPKAAWLSRYAFAFIV
                     GIGAGLAIPRTISSFILKQIEDTVRPLLAVAPGGGITFDYSLLNPASHVNAIIILIGV
                     VSVLFYFFFSVEHSGPGKAVARAGILFLMISFGAAFGYTVMARMSLLIGRLTDLIEFS
                     DASYGRPTLWLVLLTVATLIVLSRRGSAHPPK"
     assembly_gap    1198..1297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1433..>2598
                     /gene="amrB"
                     /locus_tag="PKK81_12990"
     CDS             1433..>2598
                     /gene="amrB"
                     /locus_tag="PKK81_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNV33850.1"
                     /translation="MESTPTIAAKDPAQYPLLRNLQFSPIKEGEEQYIVLWDPTGLSK
                     ERLVLPLNYFFIVQHLDGEHSVQDIGAIYLKRFGEFLMPNKVEQLLADLDTKLFLEGT
                     RTEQAKQQALTAYRQAPARLPQFSGRSYEADAAKLRAQINGFFTSKEGPEVKASEHAG
                     KLIKGLVAPTYELKHAGPIYAWAYKELQESQQPDLYVLIGTAYSGLEQPVAMTDKDFE
                     TPLGLVKVERTVTDRLREQIPSAFTDELAHHNEHALEFQLPFLQESLGKDRPFTIVPI
                     LTSFSADSLRDPQIREQVETFLQALKDVLVATGKNYCVVVGAELAHIGMRYGDSAPPT
                     DFSFHRCMQTDLEMLKHVENRDPEEFAKFIQKENDQRRISGFSPIYSLLRLIQA"
BASE COUNT          500 a          799 c          687 g          512 t
ORIGIN      
        1 tgaacatggg gcaggacctc tacaacgcct tcccggcgga ctatggcggc aaggcaacca
       61 agggcctgcc ggtgctggcc ggagtgcaga acctgcgcga tgtgacctat gcagtgagtt
      121 tgggcgcagg caaccccggg gtcgaagcct ggtacgtctt cggcaaagac aagtacaagt
      181 tcgaactcgg cggcggctgt accggcgtca tcgcgcccgg cctctatccg ctgctcagaa
      241 gcggccagat caacgggttg atcggcggtt tgcgcggagc ggcggaatac gaaaccctga
      301 tcgggcagaa ggggaaagcg gtcgccggca tggatgcgca atcggccacg cacctcgcca
      361 ttatcgtgct cgtcgccatc tgtaacatct tctactttac gttgcggcgg cagcagcgcc
      421 ggcacgacgg gttaggatcg tagccgcatg gatctttcct tcgacgtcat actcggcgcc
      481 tggatcgcca cagggttgac cctcttcatc ctgtcgttcc tgtacgaaga caatccgctg
      541 ttcaaactcg ccgaacatct ctacgtcggc gtgtcgctcg gctatacgat cgtcaaaacc
      601 tacgacacgg tggtcatgac cctcatcctg cgcccgatcc tcgacaaggg cgaatggtcg
      661 ctgctgatcc ccgtcgccat cggcatgctc atgctgaccc ggtatgtgcc caaagccgcc
      721 tggctctcgc gctatgcctt cgcctttatc gtgggcatcg gcgccggtct cgccatcccc
      781 cgcaccattt cgtcgtttat tctgaagcag atcgaagaca cggttcgccc gctcctggct
      841 gtggccccgg gcggcggcat cacgttcgac tattcgctgt tgaatcccgc gagccatgtg
      901 aacgccatca tcattttgat cggagtggtg tccgtcctgt tctatttttt cttctcggta
      961 gaacattcag gaccgggcaa ggctgtcgcg cgcgccggca tcctctttct gatgatctcc
     1021 ttcggcgcag ccttcgggta taccgttatg gcccgcatgt cgctcctgat cggccgcctg
     1081 acggacctga tcgaattctc cgacgcctcc tacggacgcc ccacactctg gctcgtcctc
     1141 ttgaccgtcg ccaccctcat cgtgctgagc cggcgcggca gcgcccatcc gccgaaannn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt cgttcgcgat tcgtgccaag
     1321 acacgcatct tctttcctca cgagagacgt ttcacctttc acgttttacc tttcacgacg
     1381 ggcttgccca ccttgcgcct gagaagttgc ctccgctaca atgccgctca ttatggaatc
     1441 aacgccgacc atcgccgcca aagatcccgc acaatacccg ctgttgcgta acctacaatt
     1501 ctcgcccatc aaggaagggg aggagcagta catcgtcctc tgggacccga ccgggttgag
     1561 caaggaacga ctggtcctgc cgttgaatta tttcttcatc gtccagcatc tcgacgggga
     1621 gcacagcgtc caggacatcg gcgcgatcta cctgaagcgg ttcggcgagt tcctgatgcc
     1681 gaataaggtc gagcagctac tggccgatct ggatacgaag ttattcctgg aagggacgcg
     1741 caccgaacag gccaagcaac aggcgttgac cgcctatcgc caagcgcccg cccgattgcc
     1801 gcagttttca ggccgcagtt acgaagccga cgccgccaaa ctgcgcgccc agatcaacgg
     1861 cttcttcacc tccaaggaag gaccggaggt caaggcatcc gaacatgcag gcaagctcat
     1921 caaggggctc gtcgcaccga cctacgaact gaaacacgcc ggaccgatct atgcctgggc
     1981 ctataaggaa ttgcaggaat cccagcaacc ggatctgtac gtgctcatcg gcaccgccta
     2041 ctcggggctc gaacaaccag tggcgatgac ggacaaggat ttcgagacac cattggggct
     2101 cgtgaaggtc gaacgcacgg tcaccgatcg cctgcgcgag cagattcctt cagcctttac
     2161 cgacgaattg gcacatcaca atgaacatgc attggaattt cagctgccgt tcctgcaaga
     2221 gagtctcggc aaggaccggc cgtttacgat tgttccgatc ctcacgtcgt tttcggcgga
     2281 cagcctgcgc gatccgcaaa tacgggagca ggtcgagacg tttcttcagg ccctgaaaga
     2341 cgtcctcgtg gccaccggta agaactactg tgtggttgtg ggcgcggaac tggcccacat
     2401 cggcatgcga tacggcgact cggcgccgcc gacggatttc tccttccacc gctgcatgca
     2461 gaccgacctc gaaatgctca aacacgtcga gaaccgcgac cccgaagagt tcgcgaagtt
     2521 catccagaaa gagaacgacc agcgccgcat ttccggcttc tcgccgatct actcgctgct
     2581 gcgtttgatt caagcgga
//
LOCUS       DAOQRM010000248         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_47703_length_2587_cov_13.340442, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000248 DAOQRM010000000
VERSION     DAOQRM010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47703_length_2587_cov_13.340442"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..669)
                     /locus_tag="PKK81_12995"
     CDS             complement(25..669)
                     /locus_tag="PKK81_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV33851.1"
                     /translation="MSKPRVLLADDHSLVLEGFRRILDDQCDLVGMVEDGRALLEAAQ
                     RVQPDIVILDVSMPLLNGIDAATQLKKSHPSIKVIFVTMHADADYVRSAFEAGASGYL
                     LKRSAVDELEQAIRAVWAGHTYITPLIAKGLLDVLMTIGPGQSQQKKTLTFRQREVLQ
                     LLAEGRTVKDIAIRLKISTRTVEFHKAQIMEQLNLRTTAELIKYALTHGIVAPS"
     gene            complement(662..1828)
                     /locus_tag="PKK81_13000"
     CDS             complement(662..1828)
                     /locus_tag="PKK81_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNV33852.1"
                     /translation="MTQPSHRLLLLTIVILGIGLYSLDLYLPLGIGNGVLYGGLVVLS
                     LASPDRKIPLLVAGACSVLAISDVFLGVRFPNVPLWMGISNRLFSLVAIWFPVVYAFQ
                     RRKIEEALRDAHGALEVRVEERTHELALVNQALLGEIVERMETERSLRESERSLKVSQ
                     QELQQSREELRALAGRLLTAQEGERRRIARDLHDDVNQRLAMLAMDLRRIEKGDQTDP
                     AAVASMVRSITGRLTAVSDDVRQMAYRFHPSILDDLGLTKAVRRLVDDFSASTGIQAV
                     YVHHDPVSPVPTDLATCVYRIAQESLNNVARHARASEVEVELICDEGRVTLSIRDNGV
                     GFDPLQVSQMRGRLGVLSMKERVRLVRGTLDVTTAPGRGTHIEVDVPLAGNAHV"
     gene            complement(2043..2555)
                     /locus_tag="PKK81_13005"
     CDS             complement(2043..2555)
                     /locus_tag="PKK81_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HNV33853.1"
                     /translation="MNKGLLIALTGVLMGVQAGVAHAVNPDNGPGCGLGKLAWADYKT
                     PKNIAPQVMMATTNGTFGSQTFGISFGTSGCTNDGKVMAGQQTNVFVAGAFESLSEDM
                     ARGGGEHLATLATLMGVPSEQQSTFFALAQQQYGALVGAGELSSVAVVKALQEAMKEQ
                     PVLAQASGIR"
BASE COUNT          537 a          848 c          698 g          504 t
ORIGIN      
        1 ctctcgtgga taccgaaggg agcgttagga aggggctaca atcccatgcg tgagcgcata
       61 cttaatcaac tccgccgtgg tacggaggtt gagttgttcc atgatctgcg ccttgtgaaa
      121 ctcaacggtt cgggtcgaaa tcttgaggcg aatcgcaata tcctttaccg ttcggccctc
      181 agccagcagc tgcaatactt cacgctgacg gaacgtcagt gtttttttct gctgtgactg
      241 ccccgggccg atcgtcatca gcacgtcgag cagccccttc gcgatcaacg gggtgatgta
      301 ggtatgcccg gcccacaccg ctcgaatcgc ctgctccagt tcatcgaccg ccgatcgttt
      361 caggagatag cctgaggccc cggcctcaaa cgccgaccgg acatagtccg catcggcatg
      421 catggtgaca aagatcacct tgatggacgg atgcgatttt tttagctgtg tagccgcatc
      481 gatgccgttc aggagcggca tcgacacatc gagaatcaca atgtcgggct ggacccgttg
      541 tgccgcctca agaagcgccc gtccgtcctc gaccatcccc accaggtcgc attgatcgtc
      601 gagaatgcgg cgaaaccctt cgagcaccag cgaatgatcg tccgccagca acacacgcgg
      661 tttagacatg cgcattccct gccagcggca catccacctc aatgtgcgtc ccgcgcccgg
      721 gcgccgtcgt cacatccaaa gttccccgca ccaaacgcac ccgctctttc atgctgagca
      781 cacccaatcg accccgcatc tgtgacacct gcagcggatc aaacccgacg ccgttgtcgc
      841 gaatagaaag agtaaccctc ccttcatcgc aaatcaattc gacctcgacc tcggaggccc
      901 tggcgtgacg cgccacgtta ttgaggcttt cctgtgcgat ccggtacaca caagtagcca
      961 ggtcggtagg gacagggctc acggggtcat gatggacata caccgcctga atcccggtgc
     1021 tggcggaaaa atcgtccacc aaccggcgca cggctttcgt cagtccaagg tcgtccagga
     1081 tcgaaggatg aaatcggtag gccatctggc ggacatcgtc cgacaccgcc gtcagccggc
     1141 cggtgatcga tcggaccatg ctcgcgacgg ccgcagggtc tgtttggtca cccttttcaa
     1201 tccggcgcag gtccatcgcc aacatcgcca atcgctggtt cacgtcatca tgcagatccc
     1261 gcgcgatccg gcggcgttcc ccctcttgag ccgtcaacag tcgaccggcc agcgccctca
     1321 gttcttcgcg actctgctgc agctcctgct gactcacctt cagcgagcgc tcgctctccc
     1381 tgagtgaccg ctccgtctcc attcgttcga caatctcccc gagcagcgcc tgattcacca
     1441 gcgccaactc gtgcgtgcgt tcctccacgc gaacctccag tgctccatgc gcgtcacgaa
     1501 gcgcttcttc aatttttctg cgctggaagg cgtagacgac gggaaaccag atcgccacca
     1561 gactaaatag ccggttgctg attcccatcc agagggggac attggggaac ctcaccccga
     1621 gaaaaacatc cgagatggcc aggacggagc aggcgccggc cacgagcagg ggaatcttgc
     1681 gatccggaga tgccagcgac agcaccacca ggcctccgta cagaaccccg ttgccgatgc
     1741 ctaatggaag atagagatca aggctgtaga ggccgattcc gaggatgact atcgtcagaa
     1801 gcagcagtct gtgggatggc tgggtcatcg gaggacaatt atcctcctgt caggctgagc
     1861 gcttcaagcc ggtgcgtctc gccggaggtg ggaaggagga gccccaaggc gcactccgcc
     1921 tcccgaagac gtgagccgct tgccggggcg cggctggcag cggaggcaga gcacgcccat
     1981 ccaccgccag acgcacacat cccggcgacc ttctccaaga gaaactgaag agggagaccc
     2041 gctcagcgga tgcctgatgc ctgcgcgagt accggctgtt ctttcatcgc ctcttgcagc
     2101 gccttgacga cggcgactga agaaagttca ccggccccaa caagcgcacc atactgctgc
     2161 tgagccagtg caaaaaacgt ggactgttgt tcggacggca cgcccatgag tgtcgccagg
     2221 gtcgccagat gttctccgcc ccctctggcc atatcctcag agagggattc gaacgcgccg
     2281 gcgacaaaca cgttcgtctg ctgcccggcc atcaccttcc cgtcattggt acagccggat
     2341 gtaccaaaac tgatcccgaa cgtttgactc ccgaacgtac cgttcgttgt cgccatcatg
     2401 acttgagggg caatgttctt cggcgtcttg taatccgccc aggccaattt acccaatccg
     2461 catccgggtc cgttgtccgg attgacagcg tgggccacgc cggcctgaac acccatcaac
     2521 acccctgtca acgcgattaa caatcctttg ttcatgccat ctcctttctg tacttgcgcg
     2581 tcaccgc
//
LOCUS       DAOQRM010000249         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_51440_length_2468_cov_16.987567, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000249 DAOQRM010000000
VERSION     DAOQRM010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51440_length_2468_cov_16.987567"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1676)
                     /locus_tag="PKK81_13010"
     CDS             complement(132..1676)
                     /locus_tag="PKK81_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNV33854.1"
                     /translation="MASKFIALPKSVSEKSFAAAIAEFRRIVGQDSVLVTAEQLAPYI
                     KTMMPVPDADHTPSAALLATTVEQIQKIVGICNTYKVPIWMISTGRNFGYGSAAPAER
                     GQVVLDLKRMNRILEVDPDLCFALVEAGVTYKQLYDYIQEHKYPLWLSCPAPSAIAGP
                     TGNTLDRGVGYTPYGEHFLFACGMEVVLANGEVLRTGMGSLPKSNTWQVFKWGYGPYL
                     DGIFTQSNYGIVTKFGFWLMPAPPAFKPFVIQYENEDDIVEIVETIRPLRISGVIPNA
                     IVIAHALYEAPTKAKRSDYVTGPGAITDEAVRQIMKDHKLGVWNVYAALYGTQEQIDV
                     NWKIVTDAFSKSGKAKILTEEEAGDDPAFQYRAALMRGDMTLKEFSLYNWRGGGGSMW
                     FAPVSQARGSETLKQMAMTKRILGKYGLDYSGEFIVGMRDMHHIVDVLYDKTDPAMTK
                     AAYQCFDELLTEFSNEGYGTYRVNTAFMDKVAHTYGPVQRHVHKTLKKALDPNGILAP
                     GKSGIR"
     gene            complement(1695..>2468)
                     /locus_tag="PKK81_13015"
     CDS             complement(1695..>2468)
                     /locus_tag="PKK81_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33855.1"
                     /translation="AKRCLLFLGDNYVDERFANGVRAACQEVGYDGLETRQVDGGLLS
                     DPGKLVSLLEQTKGARWVAVMDDASAAVFQELGRTAGGRLLSVGSHASAKDDACPLRH
                     TWLAASSAQGAGGVLATRLIGAGESFSITESFLDGSSAASKLTNWSASGFSSYRSAGS
                     DAIHLHCSGLSLLEGCAQLGLAGVEGWTPIPSHVSQREVVSRQSPQWVESVGYAVAAS
                     ALGIQSIPESCSSRAFVHRAFNPHSLPPTQRFVSFVMDI"
BASE COUNT          501 a          753 c          710 g          504 t
ORIGIN      
        1 ggaacggccc gccctttttg cggaccaccg ccgctgtgat ctctcgggtc tgtatcctgg
       61 gtatgttggt catctgttac tccttcatgc gcgatcgtca tcgcatcgac tgaacagaac
      121 gagtcgccgg gctatcgaat gccggatttg cccggcgcaa gaatcccatt gggatcgagc
      181 gccttcttga gcgtcttatg gacgtggcgt tgcaccggac cataggtgtg cgcgacctta
      241 tccatgaagg ccgtgttgac ccggtaggtg ccgtaacctt catttgaaaa ctctgtcagc
      301 aactcatcga aacactggta ggcggccttc gtcatggccg gatccgtttt gtcatagagc
      361 acatccacga tgtggtgcat gtcccgcatg ccgacgatga actcgccgga ataatccagt
      421 ccgtacttcc ccagaatacg tttggtcatg gccatctgct tgagcgtctc gctcccgcgc
      481 gcctgcgaga ccggtgcgaa ccacatggag ccgccgccgc cgcgccagtt gtagagcgag
      541 aattccttga gggtcatgtc gccgcgcatc aacgccgcgc gatactggaa ggccggatcg
      601 tcgcccgcct cttcctccgt gaggattttc gccttgccgg atttgctgaa cgcgtccgtc
      661 acgatcttcc aattcacgtc gatctgttcc tgcgtgccgt acagcgccgc gtagacgttc
      721 cagactccca gcttgtggtc cttcatgatc tgccgcaccg cttcgtcagt gatcgccccg
      781 ggcccggtca catagtcgct tcgcttggcc ttcgtcggcg cttcatagag cgcatgggcg
      841 atcacgatcg cattggggat gacgccgctg atgcgaagcg gcctgatggt ctcgacgatt
      901 tccacgatat cgtcttcgtt ctcgtactga atgacgaacg gcttgaaggc cggaggggcg
      961 ggcatcagcc agaatccgaa cttagtgacg atgccataat tggattgggt gaagatcccg
     1021 tcgaggtacg gaccgtaacc ccacttgaag acctgccagg tattggactt cggcaacgag
     1081 cccatcccgg tgcgcagcac ctcaccattg gccagaacca cctccatacc gcaggcaaag
     1141 aggaaatgtt ctccgtaggg cgtatacccg acgccgcgat ccaacgtatt gccggtcggt
     1201 ccggcaatgg cggaaggggc gggacaggag agccacagcg ggtacttatg ttcctggatg
     1261 taatcgtaga gctgtttgta ggtgacgccg gcctcgacga gggcgaaaca gagatccgga
     1321 tcgacttcaa ggatgcggtt catccgtttc agatcgagca ccacctggcc ccgctcggcc
     1381 ggtgcggcag aaccataacc gaagttccga ccggtcgaga tcatccagat cggtactttg
     1441 tatgtattgc aaatgccgac gatcttctgg atttgctcaa ccgtcgtagc caggagcgcg
     1501 gcggacggag tatggtccgc atcgggaacc ggcatcatgg tcttgatgta aggggcgagc
     1561 tgttccgccg tcacgaggac ggagtcttga cctactatac gacggaattc cgcgatcgcc
     1621 gccgcaaaag acttctcgga gacgcttttc ggtaacgcga taaatttgct ggccatgctg
     1681 ttcctcctgt ccgcctaaat atccatcacg aatgagacga atcgttgtgt gggtggcaac
     1741 gaatggggat tgaatgcccg atggacgaac gcgcggcttg agcaggactc cggaatcgac
     1801 tggatcccga gagccgacgc cgccacggca taacccacgg attccaccca ctgtggagac
     1861 tgccgcgaga ccacctcgcg ttgggagacg tgcgatggga tgggcgtcca tccctcaact
     1921 ccggccaggc ccaactgtgc acatccttcc aagagcgaca gtcccgaaca gtggagatga
     1981 atcgcgtcgg acccggcaga ccgataggag gaaaatcccg atgcagacca attggtcagc
     2041 ttgctcgcgg ccgatgatcc gtcgaggaag ctttctgtaa tcgaaaaact ttcacccgca
     2101 ccgatcagtc gtgtagccag gacaccaccc gcgccctgag cagatgaggc cgcaagccac
     2161 gtatgtcgaa gcggacaggc gtcatccttc gcagaggcat gagaaccgac cgacagcagc
     2221 cggcctccgg ctgtccgacc gagttcctga aagaccgccg cactcgcatc gtccatgacg
     2281 gcgacccagc gcgcgccttt cgtctgttcg agcaatgaca ccagcttgcc gggatcggac
     2341 aggagcccgc cgtctacctg tcgcgtctcc agcccgtcgt atcccacttc ctgacaggcc
     2401 gcgcggacac cgttcgcaaa ccgttcgtct acgtagttgt ctcccaggaa gagcagacac
     2461 cgcttggc
//
LOCUS       DAOQRM010000250         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_52745_length_2429_cov_16.689975, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000250 DAOQRM010000000
VERSION     DAOQRM010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52745_length_2429_cov_16.689975"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..122
                     /locus_tag="PKK81_13020"
     CDS             9..122
                     /locus_tag="PKK81_13020"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33856.1"
                     /translation="MDRHIPVDVRISGCPPRPAEILAVLGALRKNKADSDQ"
     gene            782..871
                     /gene="kdpF"
                     /locus_tag="PKK81_13025"
     CDS             782..871
                     /gene="kdpF"
                     /locus_tag="PKK81_13025"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HNV33857.1"
                     /translation="MNAMYALGAVLSLGLLAYLMIALLKAEWF"
     gene            871..>2429
                     /gene="kdpA"
                     /locus_tag="PKK81_13030"
     CDS             871..>2429
                     /gene="kdpA"
                     /locus_tag="PKK81_13030"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNV33858.1"
                     /translation="MTVNGLAQIGLFFFVLVALVKPLGWYMARVYTGQACGMDRVMGP
                     FERLIYRVCGVRATEEMDWKTYAVAMLLFNVTGLVALYVLQRLQGYLPLNPTGFGAVA
                     PDLAFNTASSFITNTNWQAYAGESMLSYLTQMLGLTVQNFVSAATGMAILVALIRGLT
                     AQTAATIGNFWVDLTRSTLYILLPLSAVLALLLVSQGTVQTFGSSHHTTLLQSVTYEK
                     PIVDAAGQPVLDEKGAARTESTAGTEQTLAVGPVASQVAIKHLGTNGGGFFNANAAHP
                     YENPTPLTDFMLILAETVIAAALTYTFGTMVGDTRQGWAILAAMLSVLALFVLGAYWA
                     ESAGNPRLTMLGVEQTAGSAQSGGNMEGKEVRFGVARSALFATVTTATSTGAVNAMHD
                     SFTPLGGLVPLFMMQFGEVIVGGVGSGLYGMLIFAIIAVFIAGLMVGRTPEYLGKKIE
                     TYEMKMASLLILIMPIIVLGFTAVAVVTESGTSSILNPGAHGFSEILYAYTSQGNNNG
                     SAFGGLNANTP"
BASE COUNT          464 a          677 c          719 g          569 t
ORIGIN      
        1 cggggccggt ggatcggcat atccccgtcg atgtgcgtat ctccggttgc cccccacgcc
       61 cggccgagat cctggcggtg ctgggagcgt tacgtaaaaa taaggcggat tcagatcagt
      121 agcgcccgga gacagtcacg cctctcccgt tcatccaaca aatcttttcg acctgctcaa
      181 tagacggtca cacgttcgcc tacatccctt cttcagcgcc gccctcgcga gttgatgtgt
      241 ttcgactctt ccgtcatctg aagcgatccg ccccaattgc gtgaacagct gtccgggaag
      301 atcttttcag aaaatccatt ttcttagcct ttttacaccg tttttacttt ctgcgcgtta
      361 ccttgagtgt ggagtggaag agaagaggcg tgaagagatg tggccggttg gcgaccgctt
      421 ggataggtgt ttcgggcgaa gcgcgccaca ctaagtttta cgtcccggta aggagcatgt
      481 acttggacct catctacctg acgatcgccg cggtattttt tctgttatcc ggctggctca
      541 tctcggcgtt ggaccgtctc tagcggccta cgggaaaact ttgttgtcac acgtgaaatt
      601 tattgctccg gaggggcagt ctaatagaac agcatcaccg caggatgctc aaaaaggctg
      661 tccagcaagg ccccagcgaa tgaaggtccg aggcgtaccc ttggggtacg ttgagggtct
      721 gaatgatgcg agaacgaagc tggcgggctt tctcggaatc ctgctctagg ggggtgtggt
      781 catgaatgcg atgtacgcgc tcggtgcagt gttgtcactg gggttgttgg cgtacctcat
      841 gatcgcgctg ctgaaggcgg agtggttctg atgactgtca acgggttggc tcaaatcgga
      901 ctcttctttt tcgtcctggt agcgttggtc aagccgctgg gttggtacat ggcacgcgtc
      961 tatacgggcc aagcctgcgg aatggaccgg gtgatgggac ccttcgaacg cctgatttat
     1021 cgtgtctgcg gcgtgcgtgc aaccgaggag atggattgga agacctatgc cgtggccatg
     1081 ttgttgttca atgtcacggg gttggtcgcg ctctatgtgt tgcagcgact gcagggatat
     1141 ctgccgctga acccgactgg tttcggggcg gtggctcccg acctcgcctt caacaccgcc
     1201 tcgagcttca tcacgaatac gaactggcag gcctatgccg gtgagtcgat gctcagttat
     1261 ctcacgcaaa tgctcggcct gacagtccaa aacttcgtct ctgccgccac gggcatggcc
     1321 atcctggtcg ccctcattcg cgggttaacc gctcaaacgg cagccaccat cggcaatttc
     1381 tgggtcgatc tgacccgcag cacgctgtac atcttactac cgctgtcagc cgttctcgcg
     1441 ctgcttctcg tctcgcaagg gacggtccag accttcgggt cttctcatca caccacgctt
     1501 cttcagtccg tgacctatga gaagccgata gtcgatgccg cagggcagcc ggtgttggac
     1561 gagaagggcg ctgccaggac cgaatcaaca gcagggactg aacaaacctt ggccgtcggt
     1621 ccggttgcgt cacaagtggc catcaagcac ctcggaacaa acggtggcgg attttttaat
     1681 gccaatgccg ctcatcccta tgaaaacccg acgccgctga ccgatttcat gctgatcctg
     1741 gctgaaaccg tgattgccgc tgctctgacc tacacgttcg gaacaatggt cggcgacacc
     1801 aggcagggct gggcgatcct cgctgcgatg ctgagcgtgc tggccctctt cgttttgggg
     1861 gcctactggg cggagtccgc aggaaatcca cgcctgacta tgttgggcgt cgagcagacc
     1921 gccgggagtg cgcaatccgg cggcaacatg gaagggaagg aagttcgttt cggggtcgcc
     1981 cgttcggcgc tctttgccac cgtgacgacg gccacatcca ccggagcggt gaatgccatg
     2041 cacgattcat tcacgccact cggcgggttg gtgccgctgt tcatgatgca attcggcgag
     2101 gtgattgtgg gcggggtcgg ctcgggcctc tacgggatgt tgatcttcgc gatcatcgcg
     2161 gtgttcattg ccggcctgat ggtaggccgg accccggaat atctggggaa aaagatcgaa
     2221 acgtatgaaa tgaagatggc atctctgttg attctcatca tgcccatcat cgtcctcggg
     2281 ttcacggcag tggcagtcgt gacggagtcc ggcacgtcgt ccattctcaa tcccggcgcg
     2341 catggtttca gcgaaattct ctatgcctat acgtctcagg gtaacaacaa cggcagcgcc
     2401 ttcgggggat tgaacgccaa tacgccgtt
//
LOCUS       DAOQRM010000251         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_52861_length_2426_cov_14.018558, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000251 DAOQRM010000000
VERSION     DAOQRM010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52861_length_2426_cov_14.018558"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PKK81_13035"
     CDS             complement(<1..1121)
                     /locus_tag="PKK81_13035"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNV33859.1"
                     /translation="MLITPEPAARSSYRLSKSRYLSGLQCHKRLYLEIHAPALATKPD
                     AATQAILDMGTDLGELARRRFPGGRLVTAGYRQSQEALAQTAELLQDSTVSAIFEGAF
                     QFDQVLIRVDVLERVGVNESGQPTWRLVEVKSSTKLKPIHVEDLTIQSYVLEGAGLVL
                     DDICLMHVNTQYVFDGVELDLGQLFSLRSLNETIRPRLPDVPSRLTSMHAALSEMSAP
                     PIAPDEHCHSPYECPFWAHCTQEKPPRWIYHLPGSSKTVIQLRELGVETIDEIPDHVT
                     LTPVQRRVRDNREWIGEGLRSALEKIVYPVHHLDFETFMPAVPKFGDTRPYQVIPTQW
                     SNHIEHPEGRLDHAEYLCRDGRDPREELAVTLLDSLGGEG"
     gene            1217..>2426
                     /locus_tag="PKK81_13040"
     CDS             1217..>2426
                     /locus_tag="PKK81_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNV33860.1"
                     /translation="MANGVAQIRRSVMTLALPVTVSSLLQRTEGIVAVFLVGGLGAIP
                     IAAVGLGQLLAFIATTLVSGLSVGTNVIIAQQWGARRYEEAGQASRHFLGLSIFVSFA
                     LALLGLSANGLIMQLLGAQPEVIGLALPYSNLIFLVIPFTVLLAVLSSILQGTGDTKT
                     PMYAMIMVNVLHIAIAYPLIYGQWGLPAFGVKGAAVAVGIAEATGSVYLLLRCRTILK
                     PSKRLRWDMLRTIWQVGAPVSGERIVQQAGILLYTKIVLIYGTVSYAAHQVGLSIESL
                     SFLPGYGFAIAAATMVGQSIGAGKYTRAKLENWEANRLATFIMSAMGIVFFFFPYALL
                     RAFTSDEAVIELGTTFLKIVALLQVPLALTMVLAGSLRGAGDTRFIMIATMIGMWGVR
                     IPIAFVAGYWL"
BASE COUNT          469 a          755 c          702 g          500 t
ORIGIN      
        1 ccttccccac ccaacgaatc cagcaacgtg acggccaact cttcacgcgg atcacgcccg
       61 tcgcggcaga gatactcggc gtggtcgagc ctcccctcag ggtgttcgat gtggttggac
      121 cattgagtgg gaatcacctg atagggccta gtatccccga atttcggcac ggccggcatg
      181 aaggtctcga aatccaaatg atgcacggga tacacgatct tctccagcgc cgaccgcaag
      241 ccttccccga tccattcccg attgtccctc acccgtcgtt gtaccggcgt caacgtcacg
      301 tgatcgggaa tttcgtcgat ggtctccacc cccaactcgc gcagctgaat gaccgtcttg
      361 ctgctcccgg gcagatgata aatccagcga ggcggcttct cctgcgtgca atgcgcccag
      421 aagggacatt cgtaggggct gtgacaatgt tcatccgggg caatgggcgg cgccgacatc
      481 tcgctcaacg cggcgtgcat cgacgtcaga cgtgagggca catccggcaa ccgtgggcgt
      541 atcgtttcat ttaaggagcg gagactaaac aattggccaa ggtcgagttc cacgccgtcg
      601 aacacatact gggtgttcac atgcatgagg cagatgtcgt cgagaaccaa gccggcccct
      661 tcgaggacgt aactctggat cgtcaaatcc tccacatgga tcggcttcaa cttggtggag
      721 gatttcactt ccaccagccg ccaggtgggt tgccctgact cgttcacccc gacacgctcc
      781 aggacatcga cacggatcaa gacctggtcg aactgaaatg cgccttcgaa gatggccgac
      841 accgtcgaat cctggagaag ctccgccgtc tgcgccaggg cctcctgaga ttggcggtac
      901 ccggccgtga cgaggcgacc gccgggaaat cgccgacggg cgagttcccc gagatccgtg
      961 cccatgtcga gaattgcttg ggtggccgca tccggcttcg tggccagcgc cggagcatga
     1021 atttccaggt acagacgctt atggcactgc aggcccgaca gatatcgcga ttttgacagc
     1081 cgataggagg aacgagcggc aggctcgggc gtgatcaaca tggataggtc caggctaccg
     1141 aagccgtgcg cctcttgtcc agtcccagga gaaggacatc ctcgtccgca accgagttct
     1201 ggtaggatga cgccccatgg caaacggcgt cgcacaaatc agacgatcgg tcatgaccct
     1261 cgcgctcccg gtgacggtga gcagcctgct gcagcgaacc gaaggcatcg tcgcggtctt
     1321 tctcgtcgga ggattgggcg caatccccat tgccgctgtg gggttgggcc aactgctcgc
     1381 gtttatcgca acgacactcg tgtcgggtct ctcggtcgga accaacgtca tcatcgctca
     1441 acagtggggc gctcgccggt atgaagaagc aggccaggcc tcccggcatt ttctaggcct
     1501 gtcgatattc gtgtccttcg ccctggccct gctcggcctt tcggcgaacg gactcatcat
     1561 gcagctgctc ggagcgcagc cggaggtcat cggcctcgca ctcccctact caaacctgat
     1621 ctttctggtc atccccttca ccgtgcttct ggcagtgctc tcgtcgattc tccaggggac
     1681 cggggacacc aagacaccga tgtacgccat gatcatggtc aatgtgctcc acatcgccat
     1741 tgcctatccg ctgatctatg gccaatgggg attgccggcg ttcggcgtga agggcgcggc
     1801 cgtcgccgtt gggattgcgg aggcgaccgg atcagtctat ctgttgttgc gctgccggac
     1861 gatcctcaaa ccgtcgaagc ggctgagatg ggatatgctc cgcaccatct ggcaagtggg
     1921 tgctccggtc tccggagaac ggatcgtgca acaggccgga attctgctct acaccaaaat
     1981 cgtattgatc tacggcacgg tctcctacgc cgcgcatcaa gtcggactct caattgaatc
     2041 gctctcgttc ctgcctggct acggctttgc catcgccgcc gccaccatgg tcggacaaag
     2101 tatcggcgcc ggcaaataca ccagggccaa actcgaaaac tgggaagcga accggctggc
     2161 gacgttcatc atgtccgcca tgggtatcgt ctttttcttt tttccctatg ccctgctccg
     2221 tgccttcacc agcgacgaag ccgtgatcga gctaggaacg actttcttga agatcgtcgc
     2281 actgctgcag gtgccgttgg ccctgaccat ggtgctcgcc ggctcactgc gcggcgcggg
     2341 cgatacgcga ttcatcatga tcgcgacgat gatcggcatg tggggcgtgc gtatccccat
     2401 cgcgttcgtg gcaggctact ggctga
//
LOCUS       DAOQRM010000252         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_54607_length_2377_cov_11.438846, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000252 DAOQRM010000000
VERSION     DAOQRM010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54607_length_2377_cov_11.438846"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PKK81_13045"
     CDS             <1..696
                     /locus_tag="PKK81_13045"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNV33861.1"
                     /translation="IYLNTFQIVQWQHLICTPTRSYEMKVAIIGTGNMASGLAHTLAG
                     AKHEVIIGSRDPAKAAALAEKVGQGAQGGGIAAAAKLAEVVILAIPFGAAADAIKDAG
                     DLAGKVLVDISNPISADYKTLVIGHTTSAAEEIQALAPEAIVVKAFNTIFAQLLPAEA
                     RQGKTLQVFVASDDEAAKGQVSGLAQSVGFEAVNAGSLSNSRFIEPIGEMNIHFGYFL
                     GKGPVVAPAWVQV"
     gene            681..1757
                     /locus_tag="PKK81_13050"
     CDS             681..1757
                     /locus_tag="PKK81_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV33862.1"
                     /translation="MGAGINHASGCNGQFKAGHGIPARIIMRSLIRSALFALPLLALH
                     GSVSAAPAIEVLGKDYTFPHKLGGLPAKLSDFQDLQINTFTTGDGVKLSYWEAGSGKP
                     LVFIPGWSANGAEYINVLYLLSKHYHVYVLDPRNQGLSQHVDYGSRITRFSADLKEFS
                     EHLGLETADYCGWSMGAAVLWGYIDLFGTKGIRKLVFVDEPVSIYAHTDWSEQERLDA
                     GGMTSSVERMVAAFTTGAPTNQLVVDMKVVERALAKDSPYYENSTAFANAFVKDDPKY
                     TAKVLFDHATNDWRDVIRHKINVPTAIFSGEYSNNLPSQRWMASVIPNAKLHAYTRPE
                     QGDHFLMFKNPFKFTEDLRAFLEH"
     gene            1835..2017
                     /locus_tag="PKK81_13055"
     CDS             1835..2017
                     /locus_tag="PKK81_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HNV33863.1"
                     /translation="MPVVRVTWFEGKETATKQAVAAEITESIVRNTGTDPKYIYVIFE
                     DVAPSDWAGEGKLFGG"
     gene            complement(2125..>2377)
                     /locus_tag="PKK81_13060"
     CDS             complement(2125..>2377)
                     /locus_tag="PKK81_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33864.1"
                     /translation="SWPSTSPNSVEKALTMWTSGASPSAEPRNHQDLPEVVAGPVARL
                     TRIFKLTEFLHQTDSRSTHFRRPKDASRRVFERVHKMAA"
BASE COUNT          523 a          703 c          668 g          483 t
ORIGIN      
        1 atttatctca atacctttca aatcgtacag tggcaacacc tgatctgcac acccacaagg
       61 agttacgaaa tgaaagttgc catcatcggt acgggaaaca tggccagcgg tctggcacat
      121 acgctggctg gcgccaagca tgaagtcatc atcggtagcc gtgatccggc taaagctgca
      181 gcgctcgctg agaaagtcgg gcagggggca caaggtggcg gtattgccgc agccgccaaa
      241 ctggccgaag tcgtgatcct ggctattcct ttcggcgccg ccgccgatgc catcaaggat
      301 gcgggtgatc tggccggcaa ggttctggtc gatatcagca acccgatcag tgccgactac
      361 aagacgctgg tgatcggtca caccacttcg gctgccgagg aaatccaggc cctggcgccc
      421 gaagccatcg tcgtgaaggc gttcaacacc atctttgccc aactgctccc ggccgaggcc
      481 cgccaaggca agactctcca agtgtttgtc gccagtgatg acgaggctgc caaaggccag
      541 gtatctgggc tggctcaatc ggtcggcttc gaagcggtca atgccggctc cctgagcaat
      601 agccgcttca tcgagccgat cggtgaaatg aacattcact ttggctactt cctcggcaaa
      661 ggcccggtcg tcgcaccggc ttgggtgcag gtataaatca cgcatctgga tgcaatggcc
      721 agttcaaagc tggccacggc atcccggcaa ggatcatcat gagaagcctc atccgttctg
      781 cgctgtttgc gctgccattg ctcgccttgc acggttcggt cagtgcagcc ccggccatcg
      841 aggtgctggg caaggactac acgttccccc acaagctggg tggtttgccg gccaagctat
      901 cggacttcca ggatttgcag atcaacacat tcaccaccgg tgacggggta aaactgagct
      961 actgggaggc aggttccggc aaacccttgg tgttcattcc cggttggtcg gccaacggcg
     1021 cggaatacat caacgtactc tatctgctca gcaagcacta tcacgtctat gtgctcgatc
     1081 cccgcaacca gggactttcg cagcatgtcg attacggcag ccgcatcacg cgcttctcgg
     1141 ccgacctcaa ggaattctcg gaacacctgg gcttggagac tgccgactac tgcggctggt
     1201 cgatgggtgc tgccgttctc tggggttata tcgacctctt cggcacgaaa ggcattcgca
     1261 agctggtctt cgtcgatgaa ccggtctcga tctatgccca taccgactgg agcgagcagg
     1321 agcggctcga cgccggcggc atgacctcct cggtcgagcg gatggtggcg gccttcacga
     1381 ccggcgctcc gaccaaccaa ctggtcgtcg atatgaaggt cgtcgagcgt gcgctggcca
     1441 aggattcgcc gtactacgag aattcgaccg ccttcgcgaa cgcgtttgtc aaagacgatc
     1501 cgaaatacac cgccaaggtg cttttcgacc acgccacgaa cgactggcgc gatgtgattc
     1561 gccacaagat caacgtgccg acagcgatct tctccggtga atacagcaac aacctaccca
     1621 gtcagcgctg gatggcttcg gtcattccca atgcgaagtt gcatgcctac acccggcccg
     1681 aacaaggcga ccacttcctc atgttcaaga acccgttcaa gttcacggaa gatctgcgtg
     1741 cctttcttga gcattaagaa aatcgctacc cgccaacgca tcaggcgggc ggcacagtct
     1801 gcaaacatcc atttttcaac gtaaggagat tcttatgccc gtcgtacgcg ttacctggtt
     1861 tgaaggtaag gaaaccgcca ccaagcaagc tgtcgctgcc gaaatcacgg aaagcattgt
     1921 tcgcaatacc ggaacggacc cgaagtacat ctacgttatt ttcgaagacg ttgcgccttc
     1981 tgattgggcc ggggaaggga aactgttcgg cggttgaagc cgaacagttt caggtggaat
     2041 cgggcggtgc gatcccaggg ccatcgcact cgaatgccat aaacctcccg aaatccgcca
     2101 gaaaaatcgt tcatgctcaa caggttaggc cgccatcttg tgaaccctct caaaaacgcg
     2161 tctactcgcg tcttttgggc gacggaaatg cgtggagcgg gagtcagtct gatggagaaa
     2221 ctcagtgagc ttgaagatcc gcgtcagccg agcaacggga cccgccacga cttccgggag
     2281 atcctggtga ttgcgcggtt cggccgacgg tgacgcccca ctggtccaca tggtcagcgc
     2341 cttctcgacc gaattggggc tggtgctcgg ccaggag
//
LOCUS       DAOQRM010000253         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_57342_length_2304_cov_13.157848, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000253 DAOQRM010000000
VERSION     DAOQRM010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57342_length_2304_cov_13.157848"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..266
                     /locus_tag="PKK81_13065"
     CDS             102..266
                     /locus_tag="PKK81_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33865.1"
                     /translation="MNQRFGITVKFLISADSPEEAEEIIESACEKMATSIHEGDVSYE
                     GIEDIEEEDE"
     gene            391..1782
                     /locus_tag="PKK81_13070"
     CDS             391..1782
                     /locus_tag="PKK81_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HNV33866.1"
                     /translation="MTDHVEPSGTDSRPPGFLNRIHQSNKQYLPQWLGVPAHIHHVAY
                     RMANPPLERPNSRREFQQLLQALEISEGAIEDRFGYGFKVAANGDRLVVVWEAHTEYY
                     SYQVWHVVRDATTPLDFGPITFPGYMMPLSPLGIRVNALDLLFLPQELPLEQELPARL
                     PGAMVYGSRILGDDIVAVTSFTPDEFDRERYLICSTSEQALRQQVAKIVDTIVAIENY
                     YHLVMLPMKAFSRAVDQIHDYEQRHLYQRAVLIEQLGGTTPPTMQKWLTVLTQDLLQV
                     SRLAESMRYRLSASVPYDRIVQSNLAALQERPYPPLRQISEYVSWKITGVTEGYQQLL
                     RRIDAMEKDFEATVAVLRTHIELRLQEQNIQLQDQNMKLLISVDSTTRAQAILQRTVE
                     SLSVIVITYYLTGLGSYVLKACYEMGWLKNANVATAIFVPIAFATSFTLMTVGRKIIV
                     KRMADSAKDTAGH"
     gene            complement(1854..2216)
                     /locus_tag="PKK81_13075"
     CDS             complement(1854..2216)
                     /locus_tag="PKK81_13075"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV33867.1"
                     /translation="MDMDLRSSTRVAVTYPVRLSGDSMIGQGTLINLSGPGCAVETTL
                     PVQPGDYLELHVMAPDQARPLTVGLAKVRWATEKKAGIEFIRVRRDEQSRLQRLIGQV
                     LEESTMEANTNGRELTAA"
BASE COUNT          454 a          652 c          699 g          499 t
ORIGIN      
        1 ttggggtgag acagaagggt gacaatactc gggagcttat ggtattgagc cacagcaggt
       61 gacagacttt ctgcccccta attcgccgaa ggagtacgat tgtgaaccag cgctttggaa
      121 ttaccgtgaa attcttgatt tcagccgatt cacccgaaga ggctgaagag atcattgagt
      181 cggcctgtga aaagatggcg acatccattc atgaagggga tgtgagctac gaaggcatcg
      241 aagacattga agaggaagat gagtagtgac gagagacgtt tctgctgccg cagcggcatg
      301 gctgcggtac ccgcttcatt ctgaagcagg cctgctgggg ttggatttaa gacgcgcctt
      361 cctctggttt tattcggagc gccgggtgtt atgacggatc acgtggagcc ttccggaaca
      421 gattcccgcc cgccgggatt tttgaaccgg atccatcaat ccaataaaca gtatctcccg
      481 cagtggctcg gtgttccggc ccatattcac catgtggcgt atcgcatggc caatccgccg
      541 ctggaacgcc ccaatagccg ccgggagttc cagcagctgc tgcaggccct ggagatttcc
      601 gaaggggcca tcgaggaccg gttcggctac gggtttaaag tcgcggccaa cggtgaccgt
      661 ctggtcgttg tgtgggaagc ccacacggag tattacagtt accaggtgtg gcatgtcgtc
      721 cgtgacgcga ccaccccgct cgatttcggg ccgatcacgt ttcccggcta catgatgccg
      781 ctaagcccgc tcggcatccg cgtgaatgcg ctcgacctgt tatttttgcc gcaagagctg
      841 ccgctggagc aggaacttcc ggcgcggctg cctggggcca tggtctatgg cagccgtatc
      901 ctgggcgacg acatcgtggc ggtcacgagt tttacgccgg acgagttcga ccgggaacgg
      961 tatctgatct gttcgacctc cgagcaagcg cttcggcaac aggtcgccaa aatcgtggat
     1021 acgatcgttg ccatcgagaa ctattaccac ctcgtgatgt tgccgatgaa ggcgttttca
     1081 cgcgccgtcg atcaaatcca tgactacgag cagcgacatc tctatcagcg cgccgtcttg
     1141 atcgagcagc tcggcggaac gaccccgccg accatgcaga aatggctgac ggtcctgacc
     1201 caggacctct tgcaggtgag ccggctggcg gagtcgatgc ggtacaggct ctccgcctcg
     1261 gtgccgtacg accgtatcgt gcagagcaac ctcgcggcgt tgcaggagcg accctatcca
     1321 ccgctccggc agatctcaga gtatgtgagc tggaaaatca ccggcgtgac cgagggctac
     1381 cagcagttgc tccggcgtat cgatgccatg gagaaggatt ttgaagcgac ggtggccgtg
     1441 cttcggacgc atatcgaact gcggctgcag gagcagaata tccaattgca ggatcaaaac
     1501 atgaagttgc tgatcagtgt ggactccacc acgcgggccc aggcaatcct gcagcgcacg
     1561 gtcgagagtc tgtccgtgat cgtgattacc tactacctga cgggcttggg aagttacgtg
     1621 ctgaaggcct gttacgagat gggctggctg aagaatgcga atgtggcgac ggcgatcttc
     1681 gttccgatcg cgtttgcgac gtccttcacc ctcatgaccg tgggccggaa aattatcgtc
     1741 aagcggatgg ccgattccgc caaggatacg gcagggcact gaactctacc ccccgcatct
     1801 cccgacgtct taccctatcg cgctcttcgc tgagtcgcgc cgatgtcttc tagttaggca
     1861 gcggtcagtt cgcgcccgtt cgtgttggct tccatggtcg actcttcgag cacctggccg
     1921 atcaggcgtt gcaggcggct ctgctcatcc cgacgcaccc ggataaattc gattccggct
     1981 tttttctcgg tggcccagcg cacttttgcg aggcccaccg tgagcggtcg cgcctggtcc
     2041 ggcgccatca catggagttc gaggtagtcg cccggttgga cgggcagcgt ggtttcaacc
     2101 gcgcagccgg gtccggagag gttgatgagg gttccctggc cgatcatcga gtcgcccgac
     2161 aggcgaacag ggtaggtcac tgcgacccga gtggatgaac gaagatccat gtccatgata
     2221 aggcctcctt gccttggccc tcgttcctga gggcgtatgg accttttcgg cagggttttt
     2281 cctgaacttt agcgggactg ctcc
//
LOCUS       DAOQRM010000254         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_57519_length_2299_cov_13.114973, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000254 DAOQRM010000000
VERSION     DAOQRM010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57519_length_2299_cov_13.114973"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKK81_13080"
     CDS             complement(<1..626)
                     /locus_tag="PKK81_13080"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNV33868.1"
                     /translation="MLHRLFAGKGLIIALAVGFIAVESIVAASFLSLLHFKTSNNWAT
                     KSEQVLLELERMMSAVAGAETSQRGYIITGSDEYLAPYREAVVTLEDQIRRIGGLTRD
                     NSIQQDRVAYLATQVEQRSEEMDQAIVTRRSKGLLYAKSAVAVNQQNRTMDTIQDISA
                     QIRKEETRVLERHRADSEAWAFTTGSFAAVFFLLNAVVFTLCGIVIKMA"
     gene            982..2229
                     /locus_tag="PKK81_13085"
     CDS             982..2229
                     /locus_tag="PKK81_13085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNV33869.1"
                     /translation="MARVVIIGASIGGLPAAYEARELLAKKHKVTVISNVESFHFVPS
                     NPWVAVGWRTRKDISFALGPVLEKKGVEYIHAMAERIEPEQNRVITSKGEVPYDYLII
                     ATGPKLNFGAVPGLGPSGYTQSVCNVDHAEQAWGTYQAFLKDPGPIVVGAAQGASCFG
                     PAYEMAFILDADLRKKKLRKKVPIYFVTPEPYIGHMGLAGVGASRRLMEDEFADHSMK
                     PICNTAIKEVQPGKLLLDGGQEIPFRYSMIIPPFAGVDAVAGTPGLCNPKGFVNIDAY
                     QANPKYKNIYSVGVCVAIPPVEQTPIPTGAPKTGYMIESMVSAAVHNIKADIEDDPKR
                     ETATWNAICLADMGDTGVAFVALPQMAPRNVTWAKKGKWVHLAKIALEKYFLRKMKKG
                     VSEPYYEKVILKAMGIAKLEGPG"
BASE COUNT          487 a          630 c          708 g          474 t
ORIGIN      
        1 gccattttga tcacgatgcc gcagagagtg aacaccaccg cattcaggag gaagaacacg
       61 gcggcgaacg agccggtcgt aaaggcccag gcttcagagt ctgcccggtg gcgctccagg
      121 accctggttt cctccttgcg gatttgcgcg ctgatatcct ggatggtatc catcgtccgg
      181 ttctgttgat tcaccgccac ggccgacttg gcatagagga gccccttgct gcgacgtgtc
      241 acgatggcct ggtccatttc ctccgatcgc tgttcgacct gtgtggcaag ataggccacc
      301 cggtcctgct ggatcgaatt gtccctcgtc agccctccga tccgccggat ctggtcctca
      361 agcgtgacga ctgcctcgcg atagggtgca aggtactcgt ccgagccggt aatgatgtat
      421 ccgcgttggc tggtttcagc ccccgccacg gcactcatca tccgttccaa ctcaagcagc
      481 acctgctcac ttttcgtggc ccaattgttg gaagtcttga agtgcagcag gctcaggaaa
      541 ctcgctgcga cgatggattc aaccgctata aatcccacgg caagggcgat gataaggcct
      601 ttgccggcaa aaagacgatg gagcatgggg gaccctcctc gataatacgt ccatagtagg
      661 agtaccggcc cgtcagcggc tatgtcaacc cgacaaatcc ccctaggtat cgtaagatct
      721 gtcgtagtgt tcgtgcgaga cacagtggaa tgagggatca ctaccccgag cccagtcttg
      781 gagcggggta taaaatttcc ccgcagccgg tttatctcta ctccggctga atccaaatga
      841 cctgtgatgg ctcttaacaa gaggagtggg aacggaccgt gcatagtccc aggtttggag
      901 cagaacgggt gactcgcggg agggccggga tcaggcagcg aggcaaggac ggtaggaatc
      961 tactttgagg ggaggtggat catggcaagg gtcgtcatca tcggtgcgtc catcggcggg
     1021 ttgccggcag cctatgaagc ccgggaattg ctggcgaaga aacacaaagt gacggtgatc
     1081 tccaacgtgg agtcgttcca cttcgtcccc tccaatccct gggtggcggt cggatggcga
     1141 acgaggaaag acatcagttt tgccctgggc cctgtgttgg agaaaaaagg cgtggaatat
     1201 attcatgcca tggcggagcg gattgagcct gaacagaatc gtgtcatcac ctcgaagggc
     1261 gaagtcccct atgactacct gattatcgcc accgggccga agctgaactt cggcgcggtg
     1321 cccggactcg ggccgagcgg gtatacgcaa tcggtctgca atgtggacca tgccgagcag
     1381 gcctggggca cctaccaagc gttcctgaaa gaccccggtc ccatcgtggt aggggcggcg
     1441 caaggcgcct cctgtttcgg accggcctac gaaatggcgt tcattctgga tgccgatctc
     1501 cgcaagaaga aactccggaa gaaggtgccg atctattttg tgacgccgga accctatatc
     1561 ggacatatgg ggctcgcagg tgtgggcgcc tcccgccgtt tgatggaaga tgagtttgcc
     1621 gatcattcga tgaagcccat ctgtaatacg gcgatcaaag aagtgcagcc gggcaaactc
     1681 ttgctcgacg gagggcagga gatcccgttc cgctactcga tgatcattcc gccgttcgcc
     1741 ggcgtggatg cggtggcagg gacgccgggg ctctgcaacc ccaaagggtt tgtgaatatc
     1801 gatgcctatc aagccaatcc caagtataag aatatctatt cggtcggcgt ctgtgtggcc
     1861 attccgccgg tggagcagac tccgattccg accggtgcgc ccaagaccgg gtacatgatc
     1921 gagtcgatgg tgtcggcggc ggtgcacaac atcaaggccg atatcgagga tgacccgaag
     1981 cgcgagaccg cgacttggaa tgcgatctgt ctcgccgaca tgggggacac gggcgtggcc
     2041 tttgtggcgt tgccgcaaat ggcgccgcgg aatgtcacct gggcgaagaa gggtaagtgg
     2101 gtgcaccttg ccaaaattgc gctggagaag tatttcttgc gcaagatgaa aaagggcgtc
     2161 agcgagccct actacgagaa ggtgattttg aaggccatgg ggattgccaa gttggaaggc
     2221 ccgggctaac ccggactatt catgaatgcc tgctccgtcg tccgattctc tcgcccggcg
     2281 gggctgttct cgttcggcc
//
LOCUS       DAOQRM010000255         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_58408_length_2277_cov_19.116562, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000255 DAOQRM010000000
VERSION     DAOQRM010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58408_length_2277_cov_19.116562"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..732
                     /locus_tag="PKK81_13090"
     CDS             103..732
                     /locus_tag="PKK81_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33870.1"
                     /translation="MASPSQDSSLTPAELTLVADGELFRAKERIILKVRDLLTELQAG
                     LQEDLGRTTLLVPPGFDPSKMQVVKGERLEECPYQYLDYPKHFLGDDKFTFRSLCWWG
                     HHLVFAIIVEGGQVKQCKKNFFDRFHQLAGQGLELSLAPTLWEWKRGEGYTLPITHDR
                     KAQLAAVLSGRAWFKIARFVPLDQPAMSEGRLSEVGRETFRSLLPLLTP"
     gene            902..1552
                     /locus_tag="PKK81_13095"
     CDS             902..1552
                     /locus_tag="PKK81_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33871.1"
                     /translation="MIAQGCRMVGQCCVAVGLALLLGCAGTGEMVSLNLQTQQPFAQS
                     SQTEPLKIVIEPFEDLRTDRSKIGQRTHLGGGVTNFNVNGGAPGVTIAEALAETLRQR
                     GWNRRGWDARVVQAGVGVSGADIVIGGEIRDFSTNAKSRVFNTKLTGESRLVIKARNL
                     ADDSSTVRNIQGEQTKLVFWFTSEDVETLMSGLLQDGIERFITDTKIEGRTLKSAK"
     gene            1598..>2277
                     /locus_tag="PKK81_13100"
     CDS             1598..>2277
                     /locus_tag="PKK81_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33872.1"
                     /translation="MRERRWETSGRLTFREVLAVGERLATLGLKPAHPAKDVICYVED
                     WAVDSPDAFDQLDHWATEDVTLLHLREAWQGDFFLLAGGYHTVYQRFQDVGTYCSISH
                     PWRTKPGLRFHHPTCMFWLGFRHNHAFIRVRLHTQEVVTPGETREDARRVEWIDERRA
                     IFLDAIGTLDLPVETSVEKGGLVLRPADPATPFFCSWPDAFGPCQFEYNSLDAYEFLV
                     PASRLAAT"
BASE COUNT          419 a          643 c          736 g          479 t
ORIGIN      
        1 tgctgaaaaa gtccgctggc atctcgcgca acagccgtga aacgtgaaat gtaaaacgtg
       61 aaacgtacct tgtcggaccc gacccgtctc ggatcacgaa cgatggcttc tccgtcacaa
      121 gactcctcgc tgacacctgc tgaacttacc ttggttgcgg acggggagct gtttcgtgcc
      181 aaggaacgta tcattctcaa ggtgcgcgat ctgctcaccg aacttcaggc tgggttacag
      241 gaagacttgg gccggaccac gctgctggtg ccgcccgggt tcgatccatc caaaatgcag
      301 gttgtgaagg gcgagcggct cgaagagtgc ccctatcaat acctggatta ccccaaacat
      361 ttcctcgggg acgacaagtt cacctttcgc tcgctctgct ggtggggcca ccatctggtc
      421 tttgccataa ttgtggaagg ggggcaggtc aaacagtgca agaagaactt ctttgatcga
      481 ttccaccagc tggccggtca ggggctggag ctgtccctcg ctcccacgct ctgggaatgg
      541 aagcggggcg aaggctacac cctgccgatc acccatgacc gcaaggctca attggcggcc
      601 gtcttgtccg gtcgggcctg gttcaagatt gcccggttcg taccgttgga tcagcccgct
      661 atgtccgaag ggcgactgtc ggaggtgggc cgtgagactt tccggagcct cctgccgctc
      721 ctgacccctt gatcggccct gcattcgact tgttccggcc gtgcgtgttc tccgtcatcg
      781 gcattcagcg agccgcgccg acgcgtttct tccaggcctc cttcccgttt gtcatccggc
      841 tgcgagttgg gtagactgtg cgcgtctttg gggaacgtca cttactgaca gggaggcatg
      901 tgtgattgca cagggatgtc gcatggtggg tcagtgttgt gtcgcagtgg gcctggcgtt
      961 gctgctgggc tgtgccggta cgggagaaat ggtctcgctg aatcttcaga cgcaacagcc
     1021 gttcgcgcag agcagccaga ctgagccgtt gaagattgtg atcgaaccgt ttgaagattt
     1081 gcgaaccgac cggtcgaaga tcggccaacg tacccatttg ggcggtgggg tcacgaattt
     1141 caacgtcaac ggaggcgcgc cgggagtgac gattgccgag gcgctggccg aaacgctgcg
     1201 ccagcgggga tggaaccgcc gcggttggga cgcgcgcgtc gtacaggcgg gcgtcggagt
     1261 gtcaggcgcg gatatcgtga tcgggggaga aatccgtgac ttctcgacga acgcgaagag
     1321 ccgggtcttc aataccaaat tgaccggcga gagccggttg gtcatcaagg ccaggaatct
     1381 cgcggacgac agcagcaccg tgcgcaacat tcagggcgaa cagaccaagc tggtgttctg
     1441 gttcaccagc gaagatgtcg aaaccctcat gtccggcctc ctgcaagacg ggattgagcg
     1501 gtttatcacc gataccaaaa tcgagggccg cacgctgaag tcagccaaat gaagcggtcc
     1561 ggtttcggcc gtcgcgtggt gagtgcggag ggtggttgtg cgtgaacggc ggtgggagac
     1621 ctcgggccga ttgacctttc gcgaggtgct ggcggtcggc gagcggctgg cgactctggg
     1681 actgaagccc gcacatcccg ccaaagatgt gatttgctac gtggaggatt gggcggtcga
     1741 ttctcccgac gcgttcgatc aactcgatca ctgggcgacg gaggacgtca cgcttctgca
     1801 tctccgcgag gcgtggcaag gcgatttttt tctactcgca ggcggctacc acacggttta
     1861 ccagcggttt caggacgtcg gtacctactg ctccatcagc catccctggc gcacgaaacc
     1921 cgggctgcgg tttcatcatc ccacgtgcat gttctggctg ggcttccggc ataaccatgc
     1981 ctttatccgc gtgcgcctgc atacccagga ggtggtgacg cccggggaaa cgcgtgagga
     2041 cgcgcggcgg gtggaatgga tcgatgaacg gcgggcgatc tttctcgacg ctatcgggac
     2101 gttggacctg ccggtggaaa cttccgtgga gaagggcggc ctggtgttga ggccggcgga
     2161 tccggcgacg ccgtttttct gttcctggcc ggatgccttc ggcccctgtc agttcgaata
     2221 caactccttg gatgcctacg agtttctcgt gccggcgagc cggttggcgg ccacctt
//
LOCUS       DAOQRM010000256         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_59376_length_2254_cov_13.697135, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000256 DAOQRM010000000
VERSION     DAOQRM010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59376_length_2254_cov_13.697135"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="PKK81_13105"
     CDS             complement(<1..2020)
                     /locus_tag="PKK81_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33873.1"
                     /translation="MSRTTTISSNSPLRPAEPRHVLETEAAQFLAGLPVLPFSHDDIT
                     AGSESELQAAVCGISEDVDLARTIQSSNYLHNIRERTAAGESPRIVIRQLQEFLASPD
                     ATVWENSWVRFPRRLLNRTADQLFEGDLAADKRHTKGPLRTDAHRYLLMEQGEEWVRL
                     PISYLLKLTLAQVIEEIERDSPLLAIEGRRLLNHFLNDNSSPETFSFYVSPMDRAQGM
                     GTGIARETAVRFLFTHLLALYANRQFGLQESGQQVLVYASPHPPLRQRRLNGLVSDAF
                     YRELFMNPCLSGWERGEEKSQYMGLCHEVLSRSQLNAVVRLKDAGIITRNLVVLPSTS
                     NISLANNGTHLSLGSRILTQALQSGGKAFSASHEKVVGDLVIKIVEHFLPLFAGTYSA
                     APYRMNFRDFHPETALGFLPHELDFTHLRMLWRRWKKKAGLSVFGRSITPVGPLWVDR
                     ALSALFHLRGDFLPDFRLIDYLVSLLSTDQSPALNGIQGNDLRLKRDLGQLGVFDERM
                     SLYLFYKLRAESVMGFSGFEGRQHSLFWNHLDDFADAASLQALVTALAFRYIATGAVT
                     HDDIPDDPFTESERRQCIFASAIGVGTVNVREAGPNRFLARILSRTQKTRVSRRYAGH
                     LRVRVDDYRLALLATLEEDGGELIEAFQLSGHLGRLRQRLLEPESHSTA"
BASE COUNT          496 a          693 c          652 g          413 t
ORIGIN      
        1 cggcggtact gtggctctcc ggttcgagca gtcgttgacg caggcgaccc agatgtccag
       61 aaagctgaaa tgcctcgatc aattcgcccc cgtcttcttc aagcgtcgcc agaagcgcca
      121 gtcggtaatc atcgacgcgg acccgtaggt gccctgcata ccggcggctg actctggttt
      181 tctgcgtcct gctcaggatg cgcgccagga accggttcgg tcccgcttcg cgcacattca
      241 ccgtcccgac gccgatggcc gacgcgaaga tgcattgccg tcgttcgctt tcggtgaacg
      301 gatcgtctgg aatatcgtcg tgcgtcaccg cgccggttgc gatatagcga aaggccagcg
      361 ccgtcaccag cgcctgcagg cttgccgcat cggcaaaatc gtccaggtgg ttccagaaca
      421 gactgtgctg ccgcccctcg aaaccggaga atcccatcac ggattccgcg cgcaatttgt
      481 aaaacaggta gagcgacatg cgttcatcga agaccccgag ttgccccaga tcgcgtttga
      541 gccggagatc attcccctgg atcccgttga gagccggact ctgatcagtc gacaacagag
      601 acacgaggta gtcgatcaac ctgaaatcgg gcagaaagtc gccgcgcaaa tgaaagagcg
      661 cgctgagggc acgatccacc cacaagggac cgaccggtgt aatggagcgc ccgaacaccg
      721 agagaccggc cttcttcttc caccggcgcc acaacatgcg caaatgggta aagtccaatt
      781 catgcggcag gaaacccaag gccgtttccg gatggaaatc acgaaagttc atgcggtagg
      841 gcgccgcgct ataggtgccg gcaaacagcg gcagaaaatg ctccacgatc ttgatcacca
      901 agtcgcctac gaccttttca tgagaagccg aaaacgcctt cccgccggat tgcagcgcct
      961 gcgtcagaat gcgactcccc aaactgaggt gcgtgccgtt gttggccagg ctgatattcg
     1021 aggtggacgg cagcacgacg aggttgcgcg taatgatgcc cgcatccttc aaccggacga
     1081 cggcattcag ttggctgcgc gacagcacct catgacagag ccccatgtac tgagacttct
     1141 cctcgcctcg ctcccacccc gacaagcaag ggttcatgaa caattcgcgg tagaaggcat
     1201 cggacaccaa tccgttgagg cggcgctgcc gcaacggcgg atggggtgac gcatagacca
     1261 acacctgctg gccgctctcc tgcaaaccga attgtcgatt ggcatacaac gccagcagat
     1321 gcgtgaacag aaaccggacc gctgtttcac gcgcgatacc ggttcccatc ccctgcgccc
     1381 gatccatcgg agacacgtag aaagagaagg tttccggcga actgttgtcg ttcaggaagt
     1441 gattcagcag ccgtcggccc tcaatcgcca gcagcggcga atcccgctcg atttcttcga
     1501 tcacctgggc cagcgtgagc ttcaacaggt aactgatcgg aaggcggacc cactcctcac
     1561 cctgctccat cagcagatac cgatgggcat cggtccgaag tggccccttg gtatggcgct
     1621 tgtcggctgc gagatcgccc tcgaagagtt gatcggcggt tcgattcagg agccgccggg
     1681 gaaaccgcac ccacgaattt tcccaaacgg tcgcatccgg cgaggcgagg aattcctgca
     1741 actgcctgat gacgatcctc ggactctcgc cggccgccgt ccgttcgcgg atattgtgca
     1801 gataattgga actctgaatc gtgcgcgcca gatcgacatc ttcggagatg ccgcaaaccg
     1861 ccgcctgcaa ctcactctcg gaacccgccg tgatgtcatc gtgggagaac ggtagaacgg
     1921 ggagaccggc aaggaattgc gcggcctccg tttcaagcac atggcggggc tcggccgggc
     1981 gcagagggga attcgaggag atcgtcgtcg ttcggctcat gctcaacctc ccgatgatca
     2041 cccggagaaa tctacgactg atacggatcg agcctaccgc cggtctgtta cgagcgcctc
     2101 atccccatag aaatgttatg taacattaca gcagcggtga ggcccttcgt tagcccggac
     2161 tactcatgaa tgccgtcgcg aggcgtgcga taccgaagcc cgccgggcga gaggatcgga
     2221 cgacgcagca ggcattcatg aatagtccgg ggga
//
LOCUS       DAOQRM010000257         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_59377_length_2254_cov_13.340155, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000257 DAOQRM010000000
VERSION     DAOQRM010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59377_length_2254_cov_13.340155"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>2254
                     /locus_tag="PKK81_13110"
     CDS             21..>2254
                     /locus_tag="PKK81_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33874.1"
                     /translation="MKEQDQIDQEYRVEAAEIARPGIEPLIPILPWPPRVRVSGLYAA
                     SRIVTPPIPAPGPLPIQPVPLAQPMPGIAPTAAGPWQIEELLPWGFLREELRLDVDGR
                     YPQMMASGTLYGVLAKRVHWIARLTASGPNAWTGSIWFKDGDVASFPYTMVEITTTAT
                     LLSSQRKAVVRFSGGGTSVRTRTLAFKSPYFRQVEFEFDCATGVTATTHIGTHDHPNR
                     PATLPGETLTIEEVYRRVGFDVRKSGSDSIVPIGDAGTNARWSDNEMHDAMQVYWSRF
                     AGKAQWSLWVFFARLHEQGTSLGGIMFDDIGPNHRQGTAIFNESFISTAPTGDANPVA
                     WAKRMRFWTACHEMGHAFNLAHSWQKQHPPDWGTPWIPLANEPEARSFMNYPYNVSGG
                     QTAFFSDFAYRFSDNELVFMRHAPERFVQMGNADWFDHHGFEQASASPEPALKLNLRV
                     DRAQATYQFLEPVVLELKLTNIGSRPLVLEKELLSMTDHMTVIVKKRDKPARQYLPFA
                     RYCHDMQAQVVMPGEFVTDSLFISVGRNGWDIAEPGYYTIQIALHMETEDVVSEALTI
                     RVAPPRGYDEEFIAQDFFSDDVGRILNFDGSAILRRGNDTLREVSDRFGDRAVAYHAR
                     VALASPLAKDYKVLDMGDRMGEMASAKEAGGRLRRAAPRIEEARQLYTSALLEKKDQA
                     IATLGRTDYEYYLGRFRESLMEHGAVLKKEPRDVKRDAKREKNGDIEHTMRVIKETLS
                     SLKD"
BASE COUNT          458 a          652 c          704 g          440 t
ORIGIN      
        1 atcacaacag gaggtagcgt atgaaagagc aagaccaaat cgatcaggag tatcgtgtcg
       61 aagccgccga aatagcccgt ccgggcatag agcccctcat accgatcctg ccctggccgc
      121 cacgtgtccg cgtgagcggg ttgtacgccg catcccggat cgtgacgccc cccatcccgg
      181 cgcccggccc cctgccgatt cagcccgttc cactcgctca gccgatgccg ggcatcgcgc
      241 cgacagcagc cggtccgtgg caaattgagg aactgctccc ctggggcttt cttcgcgaag
      301 agctgcgcct cgatgtcgac gggcgctatc cgcagatgat ggccagcgga acgctctatg
      361 gcgtgttggc caaacgtgtg cactggattg cccgtctcac cgcatccggg ccgaatgcct
      421 ggaccggctc aatctggttc aaggatggcg atgtcgccag cttcccctac acgatggtcg
      481 agatcacgac gacggcaacc ttgctctcca gccagcgcaa ggccgttgtc cggttctcag
      541 gcgggggcac gtcggttcgg acgagaacgt tggcgttcaa atctccgtat ttcagacagg
      601 tggaattcga gttcgactgc gccaccggcg tcaccgcgac cacgcacatc ggcacccatg
      661 accatccgaa tcgtccggcc acattgccgg gcgagacgct caccatcgag gaggtctacc
      721 gaagggtcgg gttcgatgtg cggaagtccg gtagcgacag catcgtccct atcggcgacg
      781 cggggacgaa cgcgcgttgg agcgacaacg aaatgcatga tgcgatgcaa gtgtattggt
      841 cgcgatttgc cggcaaggcg cagtggtccc tgtgggtgtt ctttgcccgg ttgcacgagc
      901 agggcacaag cctgggcggc atcatgttcg acgatatcgg gccgaatcat cgccagggga
      961 cagcgatttt caacgagtcg ttcatctcta cggcgccgac cggtgatgct aatccggtcg
     1021 cgtgggccaa gcggatgcgg ttctggacgg cgtgccatga aatgggacac gccttcaatc
     1081 tggcgcattc gtggcagaag caacacccgc cggactgggg gaccccgtgg attcctttgg
     1141 cgaacgaacc ggaagcccgc agcttcatga attatccgta caacgtgtcc ggcggacaga
     1201 cggcgttttt ttccgacttc gcctatcggt tcagcgacaa cgagttggta ttcatgcgcc
     1261 atgcgccgga gcggtttgtg cagatgggga atgccgattg gttcgatcac catggattcg
     1321 agcaggcaag tgcttcgccg gagcccgcgt tgaagctcaa ccttcgcgtc gatcgggccc
     1381 aagccaccta tcaattcctt gagccggttg tgcttgagct caagttgacc aacatcggtt
     1441 cgcgtccact ggtgctggaa aaggagttgc tgtccatgac ggatcacatg accgtcatcg
     1501 tcaagaagcg ggacaagccg gcccggcagt acctgccctt tgcgcgctat tgccacgaca
     1561 tgcaggctca ggtggtgatg cccggggaat ttgtgaccga ttcgctcttt atctctgtcg
     1621 ggcggaacgg ctgggatatc gccgagccgg gatattacac gatccagatc gcgctccata
     1681 tggagacgga ggatgtcgtc tctgaggccc tgaccatccg tgtcgctcct ccacggggct
     1741 acgacgaaga atttattgcg caggacttct tctctgatga cgtcggacgc atcctcaatt
     1801 tcgacggcag cgccatactc cgtcgaggga acgatacgct cagggaggtg agtgaccggt
     1861 tcggcgatcg cgccgtggcc taccatgcgc gtgtggccct ggcgtcgcca ttggcaaagg
     1921 attacaaggt gctcgacatg ggcgatcgga tgggggagat ggcgtctgca aaggaggccg
     1981 gaggccgtct ccgtcgcgcc gccccccgta ttgaggaggc acggcagctc tatacgagcg
     2041 ctctgcttga gaagaaagat caggcgatcg cgaccttggg acggaccgac tatgaatatt
     2101 atctgggccg gttcagggag tcattgatgg agcatggggc ggtgttgaag aaagagccgc
     2161 gggacgtcaa acgagatgcc aaacgcgaaa agaatggtga tatcgagcac acgatgagag
     2221 tgattaagga gacactctcc agcctcaaag atca
//
LOCUS       DAOQRM010000258         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_62075_length_2191_cov_12.574438, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000258 DAOQRM010000000
VERSION     DAOQRM010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62075_length_2191_cov_12.574438"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PKK81_13115"
     CDS             complement(<1..650)
                     /locus_tag="PKK81_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33875.1"
                     /translation="MASQDETDRTDTANQAGLSPQAQARSAQGDGSHGLPPPQLLSDA
                     ENKQFRLKLTIFWSVVIAAIVAAFATGYPSWENYIVQEAAKRQLRAYIDVRPRGISTI
                     EEGLVPRVHDTFHNIGRTPAYDNGSFSRILVMDYPLTRRLVNDECRHVTPDPKAGKWF
                     IGTPKRPGIARESPLTAAEVDAIKGGKSAIYFHGRVCYRDIFNEPHRTDFCLYWKW"
     gene            922..2190
                     /locus_tag="PKK81_13120"
     CDS             922..2190
                     /locus_tag="PKK81_13120"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HNV33876.1"
                     /translation="MSATEQTLPTKAEDYVRMRALATGLLLFMALLFVVALFQQETHP
                     MFAWLRAFAEAAMVGALADWYAVTALFRHPLGLPIPHTAIISGNTERIASNIGSLTQR
                     KLVTPEGIARLVGSWRIPEELIEALLAPERRRALTYECAQILVRALNASEDATMQRML
                     RDIATKVMRGVSVAPLAGQMLAGFLGSHQRDRLLNDILSAVLEYVDANRDSLGRTVAE
                     KLPWSRVLSFVKLDYPVSHKVLDSVYDTLCTMRDDPHDPMRRKAIERLHELSQWLMHS
                     GEALRREASLKEKLLAYDTLLQFLDDSWHRLKQWMLDDLSQDPSDIRTYLDAALADIG
                     RTLQKDAALRAMLHDGVQGLVEALATRHSDKIGELVANTVREWSPAQMVETIEREVGK
                     DLQYIRINGTIVGGLVGLLLHALALLIGGR"
BASE COUNT          426 a          617 c          686 g          462 t
ORIGIN      
        1 tcccatttcc aatagaggca aaagtcggtg cggtgcggct cgttaaaaat gtcccggtaa
       61 cagacccggc cgtgaaaata gattgcggat tttccgccct tgatggcatc gacctccgca
      121 gccgtgaggg ggctttcccg cgcgattccc gggcgcttgg gcgttccgat aaaccacttc
      181 cccgccttcg gatccggcgt gacgtggcga cactcgtcgt tgacgagccg acgcgtcaga
      241 gggtaatcca tcacaagaat tcttgaaaac gacccgttgt cgtatgcggg agtcctgccg
      301 atattgtgga aggtgtcgtg gacgcgaggc accaggcctt cctcgatcgt actgattccc
      361 cggggccgca catcgatgta ggcgcgcaat tgtcgtttcg ccgcctcttg aacgatatag
      421 ttttcccaac tgggataacc ggtcgcaaag gccgcaacaa tcgccgcgat caccacgctc
      481 caaaaaatcg tcagtttcag ccggaattgc ttgttttcgg cgtcggagag taactggggt
      541 ggtggcaggc cgtgacttcc gtctccctga gccgaacggg cttgggcttg cggagataga
      601 ccggcctggt ttgcggtatc ggtccggtct gtttcgtctt gactcgccat ggagtactcc
      661 ccccttgtgg ttcgtaccag gaaggctcgt cacacagctc atacgcggag cgcctatgtc
      721 tccactatag gaaagtggaa ggattaaggc aacaggaaga aggtcaaggc ctggaggtct
      781 cccggttgcg caagtctgaa ggatcaacta gcctggatta ttcatgaatg ccgtcccgag
      841 gcgctcccgg cgcgcgcgtg tgagtgtgtg agtacgggtg aaactgttcg cctgctccag
      901 gtgtgagaga ggagaagagc tgtgagtgct acagagcaga ccctgccgac gaaggctgag
      961 gactatgttc gcatgcgggc cttggcaacc gggctcctgt tgttcatggc gctgctgttt
     1021 gtcgtggcct tgtttcaaca agagacgcat cccatgtttg catggctccg agccttcgct
     1081 gaggccgcga tggtgggcgc gcttgccgac tggtatgcgg tgacggcgct gttcagacac
     1141 cccctcggct tgccgattcc gcacacggcc atcatctccg ggaataccga gcgcattgcc
     1201 tctaatatcg gcagtctcac ccagcggaag ctggtcacgc cggaggggat cgcccgattg
     1261 gtcggttcct ggcgcatccc tgaagaactg atcgaggcgc tcctcgcccc cgaacgcagg
     1321 cgtgcgctga catacgaatg cgcgcagata ctcgttcgag cgctgaacgc gtcggaagat
     1381 gcgacgatgc agaggatgct gcgcgacatt gccaccaagg tcatgcgggg cgtgagcgtc
     1441 gccccccttg ccggtcagat gctggccggc tttctcggga gccaccaacg ggatcgcctg
     1501 ctgaacgaca tcctgtctgc tgttctcgag tatgtggacg ctaacagaga ctcgttgggc
     1561 aggacggtgg ctgaaaagct cccttggtcg cgggtgctca gtttcgtgaa gttggattac
     1621 cccgtctccc acaaagtcct ggactcggtt tacgacaccc tctgcacgat gcgggacgat
     1681 ccgcacgatc cgatgaggcg gaaggcgatt gagcggctcc acgaattgtc tcagtggctc
     1741 atgcattccg gcgaagcgtt gaggcgagag gcgtccctca aagaaaagct gctcgcatac
     1801 gacacgttgt tgcagttcct tgacgattca tggcatcggc tcaagcaatg gatgctcgat
     1861 gatttgagcc aggatccctc cgacattcgg acctatctcg atgccgccct ggccgatatc
     1921 gggcgcacgt tgcagaaaga tgccgcattg cgcgccatgt tgcatgatgg ggtgcagggt
     1981 ctggtcgagg cactcgccac caggcacagc gacaagatcg gggagttggt ggccaacacg
     2041 gtcagggaat ggtcgccggc tcagatggtg gagaccatcg agcgggaagt cggcaaagac
     2101 ctgcagtaca ttcgcatcaa cgggacgatc gtcggaggcc tggtgggatt gctcctgcat
     2161 gccctggccc tgttgatcgg ggggcggtaa a
//
LOCUS       DAOQRM010000259         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_62156_length_2189_cov_20.647610, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000259 DAOQRM010000000
VERSION     DAOQRM010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62156_length_2189_cov_20.647610"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="PKK81_13125"
     CDS             complement(<1..1631)
                     /locus_tag="PKK81_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNV33877.1"
                     /translation="MMPGLRSSAGALFAFLLPIVPILFPLVVIFLLATSVSAEDPPTP
                     YLTQLLQQARQARLADAREWHLLLHYRANLFGGYTSEQDDPGFFLAPEGKTDPQSELD
                     ATLRQFFSPELVGRSKQPAQCAFVARYAWLGEQLRFDPIQLPPMPCERFERWIEEFHA
                     RSVTLVFPSAFMNNPASMFGHTFLRIDQEGQTAQTRLLAYTINFAADVPPDEGLAYPV
                     RGIFGGYRGQFSTVPYYLKVQEYRDIENRDIWEYRLNLDDRQIRRLLMHAWELGNASF
                     DYFFFKENCSYHLLSLLEYADPSLHFIERFRFWTIPADTVRALAAEPSLVQEIAFRPS
                     RVTLIRRKREHLTDSEHRLVKRLVGDASAAQSQAVRSLPLSRQAFVLDVASDYVRYKA
                     ERDEAQAATARDHNRQILTARSLLRIPSEDLSIAPFAMQPELGHKTSRASLGAGWRND
                     DSYEEVGVRMAYHDLLDPEPGYTPDAQIEVGSISVRHYNRADQTRIERATLLNVLSLS
                     PIDSLFHAPSWKLNIGMQTISHRECQLCSNWNFNGG"
     gene            complement(1631..2140)
                     /locus_tag="PKK81_13130"
     CDS             complement(1631..2140)
                     /locus_tag="PKK81_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HNV33878.1"
                     /translation="MLRQLIVALGVVAVASQAGLAMAANPDTGPGCGLGKLAWADYKN
                     QKNIAPQVMMATTNGTFGSGTFGISSGTSGCTNDGQVMADQKTTMFALLNFENLTQEM
                     AQGKGEHLASLASLMGIPDEQHPAFFALTQERYTALVQDGETSSVALVKTLNDAVSKS
                     PVLAQAVSR"
BASE COUNT          492 a          664 c          611 g          422 t
ORIGIN      
        1 ccgccgttga aattccagtt actgcacaac tggcattcgc gatggctgat tgtctgcatg
       61 ccaatattta atttccacga cggcgcatga aacagactat cgatcggcga aagcgacagc
      121 acgttcagca gggtggcccg ttcgattctt gtctggtccg ctcgattgta atggcgcaca
      181 ctgatcgagc ccacttcgat ctgcgcatcc ggggtatagc caggttccgg atccagcaga
      241 tcgtgatacg ccatccgcac accaacctct tcgtagctgt cgtcattgcg ccaacctgcc
      301 cccagacttg cgcgagatgt cttgtgcccc agctccggct gcatggcgaa gggagcaatc
      361 gagagatcct ctgacggaat tcgcaagaga ctccgtgcgg tcaggatctg ccggttgtga
      421 tctcgagccg tcgccgcctg cgcctcgtcg cgctcagcct tgtaccgcac atagtcggac
      481 gcgacatcca gcacgaacgc ctggcgggag agcggcagac tccgaacagc ctgcgattga
      541 gcggccgacg catcgcccac caatcgcttc accaatctat gctccgagtc cgtcaagtgt
      601 tctcgtttac gacgaatgag cgtcacacgg gacgggcgaa aagcaatctc ttgtacgagg
      661 ctcggctctg cggcaagcgc tcgaaccgtg tcggccggaa tagtccagaa acggaatcgt
      721 tcgatgaagt gcagcgaggg atccgcatac tccagcaacg acaagaggtg gtacgaacaa
      781 ttttccttga agaaaaaata gtcaaacgac gcgttcccaa gttcccaggc gtgcatcagg
      841 agccggcgga tctgccgatc gtcaagattc agtcgatact cccagatatc gcgattttcg
      901 atgtcccgat attcctgcac cttcaggtaa taggggacgg tggagaactg cccccggtag
      961 ccgccgaaaa tgccgcgcac cggataggcg agaccttcat ccggagggac gtcggccgca
     1021 aagttgatcg tataggccag caaacgagtc tgagccgttt gtccttcttg atcgatgcgc
     1081 aagaaggtgt ggccgaacat ggaagccgga ttgttcatga aggccgacgg gaacacgagc
     1141 gtgacggatc gagcatgaaa ctcctcgatc catcgttcga accgttcgca cggcatgggt
     1201 gggagctgaa tcgggtcgaa acgcagttgc tcccccagcc aggcataccg cgcgacaaat
     1261 gcgcactgag ccggttgctt ggagcgaccg accagctccg gcgaaaaaaa ctgtctcagc
     1321 gtcgcatcga gttccgactg gggatcggtt ttcccctccg gcgcgagaaa gaacccgggg
     1381 tcgtcctgct cactcgtgta accgccgaac aaattggcac gatagtgcaa caggagatgc
     1441 cattcgcgcg catccgccaa tctcgcctgg cgggcctgtt gaaggagttg cgtcaaataa
     1501 ggcgtgggcg gatcttccgc cgagacagaa gtcgcgagca aaaaaatgac taccaaagga
     1561 aacaggatgg gcacgatggg aagcagaaag gcgaacaggg ctccggccga tgaccggagc
     1621 cctggcatca ttagcggctg acggcttgag cgaggacagg gctcttcgac accgcgtcat
     1681 tcagcgtctt caccaacgcc accgacgagg tttcaccgtc ctgcacgagc gccgtgtagc
     1741 gttcctgggt taacgcgaaa aacgccggat gctgttcatc cggaatcccc atgagcgacg
     1801 ccagggaggc caaatgctca cccttgccct gcgccatctc ctgcgtcaaa ttctcaaagt
     1861 tcaggagtgc aaacatggtg gtcttctgat ccgccataac ctgcccgtcg ttcgtgcacc
     1921 cggacgttcc ggaactaatc ccgaacgtgc cgctgccgaa ggtgccgttg gtcgtggcca
     1981 tcatgacctg cggcgcaatg ttcttctgat tcttatagtc cgcccacgcc agcttcccga
     2041 gaccacaacc cggtccggta tcaggattcg ctgccatggc caagccggcc tgggacgcca
     2101 ccgccaccac acccaacgca acgatcaatt gtctcagcat aagtcctcct cctttacaca
     2161 agtgaatgac tccgcccgat ggtccccat
//
LOCUS       DAOQRM010000260         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_62381_length_2184_cov_17.340535, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000260 DAOQRM010000000
VERSION     DAOQRM010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62381_length_2184_cov_17.340535"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1335
                     /locus_tag="PKK81_13135"
     CDS             343..1335
                     /locus_tag="PKK81_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33879.1"
                     /translation="MFHESCFVLRAFFRHAAGSLYHIVIVAMSAGIALLLPAGAGRFL
                     SFWSHVEHDKFSLIAVEMTAAVLLMGCFNYVHRSLRDRALAEAALGAGLVSFFPRRGP
                     QARRRIQALKDEQGTGRSVMVIGSSGAGTLVDQVGDLSSVLDKCLEAKILLVNPFSQD
                     ARARMQALAHPACTYPAFREEVRQSIALLKRLKAIGKVVKLKLYADAPLVKLVILGDY
                     LWLQHYHADLDIQNMPEYVLQRNRKEHGLYTFYAHYFMQRWESPELPEYDLDTDELVY
                     RSRTGQEIRRESVEIGAAPVAAHSAETARVDTREAFQLLHAPGPYLPARGYDVE"
     gene            1491..>2184
                     /locus_tag="PKK81_13140"
     CDS             1491..>2184
                     /locus_tag="PKK81_13140"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNV33880.1"
                     /translation="MGNGVERRAVSPMTTFFSYGFRPFFLGASLFAGVAIPVWILILA
                     GTGNPVLLSAYRDWHVHEMLFGFLPAVIAGFLLTAVPNWTDRPAIQGRELMLLFTLWL
                     AGRAAMAIPLVVPIFAAIVDAAFLVALVGLLWRELAAGNSWNHAPVAVAISLYAAANV
                     TFHVLTVRGAETDLALRLALALDLFLLTFIGGRLTPNFTREFLAGQGKKERPAPFSRF
                     DALSIALVLMAAL"
BASE COUNT          342 a          626 c          688 g          528 t
ORIGIN      
        1 gaagcctggt tggcgtcgcc tgatgcgatg gattggctga cggatcagtt ggcgaactga
       61 gcggagcgac tggtgtcgaa tcaccgtgtg gatacgcctc tctcagcatt cccgtccttg
      121 cgtcgcgtca tcagacctgg tacagtgcag gtgttcgcgt aagaccttct ttagccgggg
      181 gcggcagccc ttgaatcttt gaagtcttac gcgattgttg tttgtgcccg cagatgtgtc
      241 gtctccgctg agcctaccgc cgaaggttgt tcctccgtcg ttcgcactgc cagttcgtca
      301 tcgtcgtggg aatctttctt tacctgttct agaggtgcat cgttgtttca cgaatcctgt
      361 ttcgttctcc gggcattctt ccgccatgcg gcaggcagcc tgtatcacat cgtcatcgtc
      421 gccatgagcg caggcattgc gctactcctg ccggccggag ccggccggtt cctgtcgttc
      481 tggtcgcatg tcgaacatga caaattttca ctgattgccg tcgagatgac cgccgccgtt
      541 ctgttgatgg gctgcttcaa ctatgtccac cgcagtctgc gggatcgggc gcttgccgaa
      601 gctgcgcttg gcgccggcct cgtctctttc tttccgcgac gcgggccaca ggcgcgccgg
      661 cgtattcaag cgttgaaaga tgagcagggt accggtcgtt ccgtgatggt gatcggatcc
      721 agcggagccg ggaccctggt ggatcaagtc ggtgatctgt cgtcggtcct cgacaaatgc
      781 ctggaggcca agatccttct ggtgaatccg ttcagtcagg atgcgcgcgc ccgcatgcag
      841 gcattggccc atcctgcttg cacgtacccg gccttccgtg aggaggttcg gcagagcatc
      901 gcgttgctca agcgtctgaa ggcgatcggc aaggtcgtga agctgaagct ctatgccgat
      961 gctccgctgg tcaaactggt gatcctcggt gactatctgt ggctgcagca ttaccatgcc
     1021 gatctggata ttcagaatat gccggagtat gtcttgcagc ggaaccgcaa ggagcacggt
     1081 ttgtatacgt tctacgccca ctacttcatg cagcgatggg agagccccga gttgcctgaa
     1141 tacgatctgg ataccgacga actggtgtac cgcagtcgaa ccggtcagga gatccggcgg
     1201 gagtcggtgg agatcggggc cgctccggtt gccgcacatt ccgcggaaac tgcgcgggtc
     1261 gatacgcgcg aggcgtttca attgctgcac gcgccgggtc cgtacctgcc ggctcgcggg
     1321 tacgacgtcg agtagggcag ttgttccttc attcctcacc acctgttcgg aacatcggtt
     1381 gttccctgcc gagacatcgc ggtcgctgtg gtattactat ggcgctatgg cggtcacgtc
     1441 gatgcatcga tgtatcggcc cggatagacg agggggagga gtgagggacc atgggtaatg
     1501 gagtggaacg acgcgcagtc tcgccgatga cgacgttctt ttcatacgga ttccggccgt
     1561 ttttcctggg ggcttcgctg ttcgccggtg tggcgattcc cgtctggatt ctgatcctgg
     1621 ctggaaccgg gaatccggtc ctgctctccg cctatcgcga ttggcatgtg cacgagatgc
     1681 tcttcggttt tctccctgcg gtgattgccg gtttcctcct cacggcggtg cccaactgga
     1741 cggatcgtcc ggcgatccag ggccgcgagc tgatgctgct tttcaccctg tggctggccg
     1801 gacgtgcggc catggcgatt cccctggtgg taccgatttt cgccgcgatt gtggatgcgg
     1861 cgtttctcgt ggcattggtg ggtctgttat ggcgggaact tgccgccggg aacagttgga
     1921 accatgcgcc ggtggccgtg gcgatcagtc tgtacgccgc cgcgaatgtg accttccatg
     1981 tcctcacagt gaggggtgcg gagacggacc ttgcgctgcg gctcgcgctt gccctggacc
     2041 tctttctgct gaccttcatc ggcggacggc tcacgccgaa ctttacgcga gaatttctgg
     2101 cgggtcaggg gaagaaagaa cgacctgcgc cgttttcccg cttcgacgca ctctcgatcg
     2161 cgctggtcct catggccgcg ctgg
//
LOCUS       DAOQRM010000261         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_62803_length_2174_cov_20.124587, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000261 DAOQRM010000000
VERSION     DAOQRM010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62803_length_2174_cov_20.124587"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKK81_13145"
     CDS             complement(<1..437)
                     /locus_tag="PKK81_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNV33881.1"
                     /translation="MIRTFQGITPTVPQSCFIEETAVVIGDVVMGEECSAWFHAVIRG
                     DVNYIRIGHRTNVQDLCMLHVTHDTHPLIIGDDVTIGHNVVLHGCTIHNRVLVGMGAI
                     IMDGAVIGEDSVVGAGALITEDTVVPPKSLILGSPAKVKRPVT"
     gene            522..1976
                     /gene="rimO"
                     /locus_tag="PKK81_13150"
     CDS             522..1976
                     /gene="rimO"
                     /locus_tag="PKK81_13150"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNV33882.1"
                     /translation="MASSLITPSRAKRNKPTIGFVNLGCSKNQVDSEVMLGTLVAGGF
                     QLTGDARAAEVVIINTCGFIEEAKQESINSIIEHGRLKKSGSCRVLIAAGCLAQRYQG
                     ELLKELPELDGVVGTGEFGRIAEICRSLLAPKARQQRLWIGQPPYLYDAETPRIRLGT
                     PHSAYLKIAEGCNRNCAFCAIPIMRGKQRSRPIESIVAEAGRLVQEGVKELNLISQDT
                     INYGVDLGLKQGLTALLRSLVTVKDVRWIRPFYLYPQQVTDELLDLYAGEARITKYLD
                     MPLQHISDGMLKRMHRLGDRKHVTKLVERIRAKIPGVFFRTAFIVGFPGETDAMFEEL
                     KQFILDMEFDRVAVFLYSDEEGTSAVDLDRKVDQAVMEERRNELLALQESISEAKNRD
                     YLGRTMEVLVDGISEESDRLLECRHEGLAPEIDGVVFCDRSAAKPGEFVSVTVTDVAG
                     YDLIAQPVGRADAPTAAPAVPTLLMPKKSGAGYR"
BASE COUNT          449 a          602 c          664 g          459 t
ORIGIN      
        1 tcggtgacgg ggcgttttac tttggccggt gagcccagga tgagactctt gggcggaacg
       61 acggtgtctt cggtgatcaa tgctccggcg ccgaccaccg agtcttcccc gatgaccgct
      121 ccgtccatga tgatggcgcc cattccgacc agcacacgat tgtgaatggt gcagccatgc
      181 aggaccacgt tgtgcccgat ggtgacgtcg tcgccgatga tgagcggatg cgtgtcgtgt
      241 gtgacatgca acatgcagag gtcctgcacg ttcgtgcggt gcccgatgcg aatgtagttc
      301 acatcgccgc gaatgacggc atgaaaccag gcgctgcact cttcgcccat gacgacgtcg
      361 ccgatgacca cggcggtctc ttcaataaag caggactgcg ggacggtcgg ggtgatgccc
      421 tgaaatgtcc ggatcatgcg ctcaatttag gatagctgca gacggaatag caagagagct
      481 cgtttgacag agttttccgg cgtctcgtag actgccaaat tatggcctcg tcattgatta
      541 ccccatcacg ggcgaaacgc aacaaaccca cgatcggctt cgtgaacctc ggttgttcaa
      601 aaaaccaggt ggactccgaa gtcatgctcg gcacattggt cgccggaggc tttcaactca
      661 cgggcgacgc gcgcgccgcg gaagtcgtta tcatcaatac ctgtggcttc atcgaggagg
      721 cgaagcagga gtcgatcaac agcatcatcg aacatggacg gttgaagaag tccggatcct
      781 gccgcgtcct gatcgccgcc gggtgtctgg cgcagcggta ccagggcgaa ctgttgaaag
      841 aattgcctga gctggacggc gtcgtgggaa cgggcgagtt cgggcgcatc gcggagatct
      901 gtcgcagttt gctggcgccg aaagctcgtc agcagcgact ctggattggg caaccgcctt
      961 atttatacga tgccgagact ccacgcattc gcctcggaac tccccacagc gcctatttga
     1021 aaattgccga aggctgtaac cggaactgcg cgttctgtgc gattcccatc atgcggggca
     1081 agcagcgcag ccgcccgatt gaatcgattg tggcagaggc cggacggttg gttcaagaag
     1141 gggtgaagga actcaacctg atttcccagg acacgatcaa ttacggagtg gatctcggac
     1201 tcaaacaggg gctcacggcg ctgcttcgct cactggtgac ggtgaaggat gtgcgctgga
     1261 tccggccctt ctatctgtac ccgcaacagg tgacggatga gttgctggat ctctatgccg
     1321 gagaggcgcg tatcaccaag tacctggaca tgccgttgca acatatcagc gacggcatgc
     1381 tgaagcggat gcaccggttg ggggaccgca aacacgtcac caagctggtc gaacgtattc
     1441 gagccaagat tcccggcgtc tttttcagga cggcgtttat cgtcggattt cccggtgaga
     1501 ccgacgccat gttcgaggag ctcaagcaat tcatcctcga tatggaattc gatcgcgtgg
     1561 cggtgtttct ctattctgat gaagaaggca cgtcggccgt cgatctcgac cggaaagtcg
     1621 accaggcggt gatggaagaa cggcgcaatg aactcctggc tctgcaggag tcgatttcag
     1681 aagccaagaa tcgtgactac ctgggtcgca cgatggaggt cctggtggat gggatctctg
     1741 aagaatcgga ccgtctgctt gaatgcagac acgaagggtt ggcgcctgag attgacgggg
     1801 tggtcttctg tgatcgcagc gcggcgaagc cgggtgagtt tgtttcggtc accgttaccg
     1861 atgtggccgg atatgatctc attgcccagc ccgttgggcg ggccgacgcg ccgactgctg
     1921 cgcccgctgt tccgactctc ctcatgccca aaaaatcagg ggcaggctac cgataagcct
     1981 gcgctcacca gtagcctgcc tgctctatac ggccgctctt agatcttggc gctgaggtac
     2041 agggacgcgc ctctccgatt gaccagcacc agcaccgttt gccccttctt gaggtctgat
     2101 gcggcccgat cgaaatcctt catcgaggtg accggtttgc ggttgatctc ccggatcacg
     2161 tcgcccggca tcaa
//
LOCUS       DAOQRM010000262         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_63303_length_2163_cov_16.760911, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000262 DAOQRM010000000
VERSION     DAOQRM010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63303_length_2163_cov_16.760911"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKK81_13155"
     CDS             <1..287
                     /locus_tag="PKK81_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNV33883.1"
                     /translation="AKSMTKSQIADHLAGKAGITKKTAVQFLDDFAALAYREAKNAFV
                     VPGIGKLVLANRKARMGRNPQTGEPIKIPAKRVVKFRVAKMAKDSILGKK"
     gene            complement(403..675)
                     /locus_tag="PKK81_13160"
     CDS             complement(403..675)
                     /locus_tag="PKK81_13160"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNV33884.1"
                     /translation="MITITLMGQLQTTDGERDLACELPTPVTVRQVIQRQGRGLRHML
                     QLLREKKVLVTINKRIASEDSLVQDGDAVRFVGHDGAGGSGLAPSF"
     gene            810..1181
                     /locus_tag="PKK81_13165"
     CDS             810..1181
                     /locus_tag="PKK81_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33885.1"
                     /translation="MPDLQFALVVSALCTSGLETLNVPEELRRRVFDACWALVSTDPP
                     PTNPRERVLDLRFGTELTLDAIVATIRQLFAAAGISMLTWDHAPSDPTRPSSPAAEPL
                     IDRLQKLYPDPPPTADPSDRN"
     gene            1311..1868
                     /gene="rfbC"
                     /locus_tag="PKK81_13170"
     CDS             1311..1868
                     /gene="rfbC"
                     /locus_tag="PKK81_13170"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNV33886.1"
                     /translation="MQITPTPLKGLFQIEPDVFGDARGKFVEIFRESRYDAAGIDKPF
                     LQDNFSWSVRGTLRGLHYQLSRPQGKLVTVVKGTVYDVAVDIRHGSPTFGQWYGVELS
                     DSNMRQLYIPPGFAHGFCVLSEDAGFLYKCTDVYSPPDERGILWNDPALAIAWPVKTP
                     LLSAKDQAYKCLADMMTELPRYESR"
BASE COUNT          415 a          615 c          622 g          511 t
ORIGIN      
        1 tggccaagtc gatgacgaaa tcgcagattg ctgaccatct tgctggaaag gcggggatta
       61 ccaaaaagac ggctgtccag ttcctggacg attttgccgc tctcgcctat cgtgaagcga
      121 agaatgcctt cgtagtaccg ggaatcggaa agttggtgct ggccaatcgg aaggcccgca
      181 tgggtcggaa cccgcaaacg ggtgagccga tcaagattcc ggccaagcgg gtggtgaagt
      241 tccgagtcgc gaagatggcc aaagattcga tcctcggaaa gaagtagtca gcctcgttcc
      301 tctattagac tgaagggccg gtgctccgtc attgagagca ccggcctttg tgtttttgca
      361 gcggccatag gtagcggcgt gccagtgagt acgtggcgga tactaaaaag acggggcgag
      421 tccgcttcca cctgcgccgt catgtcccac gaagcgcacc gcatcgccgt cttgcaccag
      481 agaatcttcg ctggcgatgc gtttgttgat ggtgaccagg acttttttct cccgcaacag
      541 ttggagcatg tgacgaagcc cgcgtccttg ccgctgaata acctgccgaa ctgtcacagg
      601 ggtggggagt tcacatgcga ggtcccgctc tccatccgtg gtttgaagtt gtcccatcag
      661 cgtaatcgta atcatgcgac ctgtgtcctc ccagtcgatc tgccgtagtg tttcgttgtg
      721 ccgtgcagcc gagcggccca aattgactcc cccagccggc tgcgactaat attatcacca
      781 tgcaatccct gaatatccag cgaccaggaa tgccggacct ccagtttgcc ttggtggtat
      841 cggccttgtg cacgtccggc ctggaaaccc tgaacgtccc ggaggaactt cggcggagag
      901 tgttcgacgc ttgttgggcg ctcgtcagca ccgatccccc accgaccaac cctcgggagc
      961 gtgtgctgga cttgcgattc ggcacggagc tcacgctgga cgccatcgtg gccaccattc
     1021 gccagctctt cgcagcagcg gggatttcca tgctgacctg ggatcatgct cccagcgatc
     1081 caacccgacc gagtagtcct gctgcagagc cgttgattga tcgattgcag aagctctacc
     1141 ctgatccgcc tccgactgct gatccctcag accgaaacta gctgccattc ttggttctct
     1201 attggcttcg ttcattcaac atcaggttgg ggaagtggag ggtcgctttc cgcctgagat
     1261 cgaacgaggt atgatgccat ccgttctccc cctgcgattc aggaggtgtc atgcagatta
     1321 cgcccacgcc gcttaagggc ctgtttcaga tcgagcctga tgtgttcggc gatgcccggg
     1381 gaaaattcgt cgagatcttt cgcgaatcac gctacgacgc ggccggaatc gacaaacctt
     1441 ttctccagga caacttttcc tggtccgtac gtggcacgtt gcgcggactc cactatcagc
     1501 tatctcggcc gcaagggaaa ttggtcacgg tcgtcaaggg aacggtttac gatgtggcgg
     1561 tcgatattcg gcacgggtcg ccgacgttcg ggcagtggta tggagtcgag ctatccgata
     1621 gcaacatgcg gcagttgtat attcctcccg gttttgccca tggcttttgc gtcctcagcg
     1681 aggacgcagg gtttctgtat aagtgtacgg atgtctattc cccgccggat gagcggggca
     1741 tcctatggaa tgatccggct ctcgccattg cctggcctgt aaagaccccg ctgctgtcgg
     1801 cgaaggatca agcgtataag tgcctggccg acatgatgac ggaattgcct cgttatgaaa
     1861 gccgctagtc ctcggccgtt tggtcggccg attccatggc cacacaactc tttttcttag
     1921 gcgggtctcc ggcaacgtgt tgcccgtgtc ttctcttccc cttcctttcg ctcgtcgtcc
     1981 ggaatcatgg actcaggtac tttgtggcct tccttccgat gcctgagacc gagccagtct
     2041 tgccgcgcga tgggcctgca tgctatgtag gaatcattcc tgtttagtga gcgagatcat
     2101 gaagttgaat tcgcaggaga ttgaggcccg gtttcgacgg cacgcggtgc gtgtgactcg
     2161 cca
//
LOCUS       DAOQRM010000263         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_63949_length_2150_cov_20.289260, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000263 DAOQRM010000000
VERSION     DAOQRM010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63949_length_2150_cov_20.289260"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..668)
                     /gene="tsf"
                     /locus_tag="PKK81_13175"
     CDS             complement(72..668)
                     /gene="tsf"
                     /locus_tag="PKK81_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNV33887.1"
                     /translation="MASLSELVKELREKTGAGILDCQKALTENGNSIDKAIDYLRQKG
                     LAAAQKKAGRETNQGLIHAYIHAGGKIGVLIEVNCETDFVARNEQFKAFVNDLALQVA
                     AASPSYVRREEIAEDVVAKERSIYEGQAKELGKPPAAWPKIIEGKLEKFYQENCLLEQ
                     AFIKDPSVVIKDLLAQQIAKIGENMNVRRFTRFQLGQA"
     gene            complement(720..1493)
                     /gene="rpsB"
                     /locus_tag="PKK81_13180"
     CDS             complement(720..1493)
                     /gene="rpsB"
                     /locus_tag="PKK81_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNV33888.1"
                     /translation="MGVVAIKELLEAGVHFGHQTNRWNPKMKRFLFGERNGVYIIDLQ
                     QTVERMEQAYAFARDTVAAGDSVLFVGTKRQAAEILEEEAKRANQFYINQRWLGGMLT
                     NFQTIRRSIDKMKKMEATLADPTHQGHTKKELGQMQKEVVKLQKNLSGIRNMRALPGA
                     VFILDTRIEHIAILEASRLEIPVIAIVDSNCDPDHIQYPIPGNDDAIRSIKLIISRIA
                     DACLEGAHLRTQREETEFASAPAGGGAKPSANLAGVAAS"
     gene            complement(1722..>2150)
                     /locus_tag="PKK81_13185"
     CDS             complement(1722..>2150)
                     /locus_tag="PKK81_13185"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNV33889.1"
                     /translation="VCRTLALKICWDGEGVTKVVRVEVAGAKSTKAAKQIAQTVATSN
                     LVKTALFGGDANWGRVMAALGRAGVPIDPSRISLQFGGVPMVRKGQGLGRAAERQLTK
                     VFKAKEFTIRIDLAQGKAGAHMWTTDLSYEYVRINASYRS"
BASE COUNT          391 a          633 c          606 g          520 t
ORIGIN      
        1 gccatagccc tgctcaccgg ccagcatttc tccgctgact ttcagcagga tgcggcggta
       61 gtgggcagaa ctcatgcttg gcctagttgg aatcgtgtga accggcggac attcatgttc
      121 tcgccgattt tggcgatttg ttgcgccagc agatccttga tgacgacgga cggatccttg
      181 atgaaggcct gttcgagcaa gcaattttcc tggtagaact tctcgagctt gccctcgatg
      241 attttcggcc aggcggccgg tggcttgccg agttccttgg cttgcccttc gtagatgctg
      301 cgctctttcg cgacgacatc ttcggcaatc tcttcccgtc ggacgtagga cgggctggcg
      361 gccgccacct gcagcgccag atcgttcaca aacgctttga actgctcatt ccgcgccacg
      421 aagtcggttt cacaattgac ttcgatgagc acaccgatct ttccaccggc atggatatag
      481 gcatggatca agccctggtt ggtctcacga ccggcctttt tctgggccgc cgccaagccc
      541 ttctgccgga gataatcgat cgctttgtcg atgctgtttc cgttctcagt cagggccttc
      601 tggcaatcca gaatgccggc ccctgttttt tcacgtaact ctttgacgag ctcactcaaa
      661 cttgccatgg gttcctcagt tggtcgaata gaacaatgcc atcacgcgag acgcggccgt
      721 caggaggcgg ccacgccggc caaatttgcc gacggtttag cgccgccacc ggctggtgcc
      781 gatgcaaact ctgtttcttc tcgctgggta cggaggtgcg ctccttcaag gcacgcgtcc
      841 gcgatccggg aaataatcag tttgatggaa cggatggcgt catcgttgcc cgggatgggg
      901 tattgaatgt gatcgggatc gcagttcgaa tcgacgatgg caatgaccgg aatttccagc
      961 cggctggcct cgaggatcgc aatgtgctcg atgcgggtat ccagaataaa caccgcgccg
     1021 ggcagggcgc gcatgttgcg gatgcccgac aggttcttct gcagcttgac gacttccttc
     1081 tgcatctgtc cgagctcttt cttcgtatgg ccctgatgcg tcggatcagc gagggtcgct
     1141 tccatttttt tcattttgtc gatgctacgg cggatggtct ggaagttggt cagcatgccg
     1201 cccaaccagc gctggttgat gtagaactgg ttggcccgct tggcctcttc ctctaaaatc
     1261 tccgccgctt ggcgcttggt accgacgaac aagacggagt caccggccgc cacggtgtcg
     1321 cgcgcgaagg cataggcttg ttccatgcgt tcgacggtct gttgaaggtc gataatgtag
     1381 acgccgtttc gttccccgaa caggaatcgc ttcatcttgg ggttccaacg attcgtctgg
     1441 tgtccgaagt ggacgcccgc ttccagtaat tccttaatcg caactactcc cataccttca
     1501 cctccctccg aagcctgcag agcgcctggc gaacgccaac gacgaggggg atgtccccct
     1561 tattctcagg ctgcgcagcg tgcgcatcac gctggtgtgg atttaaataa gatgtccctg
     1621 attttggatg ccgcggggtg aacgatggga ctaaccgttc attgcgcgaa acgcccgaac
     1681 gctagcatag tggctgcgat tttgcaaccg cgcgacgcca cttatgatcg ataactcgcg
     1741 ttgatccgca cgtattcgta agacaaatcc gtggtccaca tgtgcgctcc ggccttacct
     1801 tgggccagat caatgcgaat ggtgaactcc ttggctttga agaccttggt cagttgccgt
     1861 tcggccgcgc gtccaaggcc ttgtcccttg cgcaccatcg gcacgcctcc aaattgaagg
     1921 ctgatgcggg aaggatcaat cggcactccg gcacgtccga gtgccgccat gacccgaccc
     1981 caattggcgt cgccgccgaa gagcgcggtt ttcaccagat tggacgtcgc gaccgtttgg
     2041 gcgatctgtt tggccgcctt cgtggacttc gctcctgcga cttctactcg tacgactttg
     2101 gtgacgccct cgccgtccca gcagattttc agagccaggg tgcggcacac
//
LOCUS       DAOQRM010000264         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_64869_length_2132_cov_20.006740, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000264 DAOQRM010000000
VERSION     DAOQRM010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64869_length_2132_cov_20.006740"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PKK81_13190"
     CDS             <1..267
                     /locus_tag="PKK81_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33890.1"
                     /translation="DAWFAWLRPLSQLMVKIDELSEEEKIEDRTEMTLAIDSVRTMLT
                     PSEEGNGFGRHYYDALQREPDVVLAHAAVRALLRTSSPGKESIT"
     gene            264..710
                     /locus_tag="PKK81_13195"
     CDS             264..710
                     /locus_tag="PKK81_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV33891.1"
                     /translation="MKAHYLGHVVFYVKDLGRSLTFYRDLLGFQEVGRIFNGAAAALT
                     SGRTHHELLLIQVGDAPGPPSGRRRGLYHIGIKVGDSLDELRAAKRELESAGVSIDGM
                     SDHTVSQSLYLRDPDGNEVELYVDADEALWKNNPSAVLAPIKPLSL"
     gene            1157..1393
                     /locus_tag="PKK81_13200"
     CDS             1157..1393
                     /locus_tag="PKK81_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNV33892.1"
                     /translation="MEEPVIAAKQPAVLELEAGTYYWCRCGRSKKQPFCDGSHKGTSF
                     TPMEFTTTEKKTVALCQCKHTKNPPFCDGTHKSL"
     gene            1439..>2132
                     /locus_tag="PKK81_13205"
     CDS             1439..>2132
                     /locus_tag="PKK81_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV33893.1"
                     /translation="MGEAASENGYSITLTERRLVAEGTMAFYFDKPTQFAFTPGQFVD
                     LTLPQPSETDAAGNTRAFSIASAPQESTLMVATRLRDTAFKRELQRMPLGSTVRMEGP
                     FGKLVLHADQTRPAVFLAGGIGITPFRSMAVQAAMQRSPHPMVLFYSNRRPEDAPFLD
                     ELQALQDKNPHYRFVGTMTEPTKSSRPWQGETGYLNAALLSKHLVNMEKPIYYVVGPP
                     GMVGALRTMLKGA"
BASE COUNT          426 a          605 c          690 g          411 t
ORIGIN      
        1 gacgcgtggt tcgcctggtt gcgcccgctg tctcaattga tggtgaagat cgatgagctg
       61 tccgaggaag agaagatcga ggaccggacg gagatgaccc tggcaatcga ctcggtgcgg
      121 accatgttga cgccgtccga agaagggaat ggattcggcc ggcattatta cgacgcgttg
      181 cagcgcgagc cggatgtggt gctggcgcat gcggcagtca gggcgctgct ccggacatcg
      241 tcgcccggga aggagtcgat cacatgaaag cgcactacct cggccatgtg gtgttctacg
      301 tgaaagatct tggacggtcg ctgacatttt atcgcgatct gcttggattc caggaagtgg
      361 ggagaatttt caatggcgct gcggcggcgc tcacatccgg ccgcacgcac catgagctgc
      421 tgctgattca agtgggcgat gcgccgggtc ctccatccgg gcgacgtcga ggcctctatc
      481 atatcggcat caaagtcggc gacagcctcg atgagctgag ggcggcgaag cgggagttgg
      541 aatccgccgg cgtctccatc gacgggatga gcgaccacac ggtgagtcag agtttgtact
      601 tgcgggatcc ggacggcaat gaggtcgagc tgtacgtgga cgcggacgaa gccttgtgga
      661 aaaacaatcc gtcggccgtc cttgcgccca tcaagccgct gtcgctctag caggatgcgg
      721 aaaaagaccg ccagcggcgt tctcgcatcg cttcgcggct caccgtaccg cacgagtacg
      781 attcgcctct tcgctcgctg cggccttgct ggacggccat tttgcgcatc ctgcgggtga
      841 ttctgatacc gaaacggcgc acgagttgat cataacgtct tgcgcaacaa tcgagtgttt
      901 ccgcagcttg ctaggcctgg ccaagacctg ccgggctgga tcacctgtgt ccctttctgc
      961 ggcaggacgc aggtggaagc tggcgcattt ctcctcaacg gtgccgatac tcgttgtgcc
     1021 ggcaagtggc agagcgagcc cttcgcctgc tggagatggc aggagatttg cctctgatgc
     1081 gtgtaagtga tcggagcccg gcggggcaag aatgtgtgcg gaagtgaaat cattcggtta
     1141 aacaggaggg cagagcatgg aagaaccagt cattgcagcg aagcagccgg ccgtgttgga
     1201 gttggaagca ggcacgtact attggtgccg gtgcgggcgc tcgaagaagc aaccgttttg
     1261 tgatgggtct cacaagggaa ccagtttcac gcccatggag ttcacgacaa ccgaaaagaa
     1321 aaccgtggcc ttgtgccagt gtaagcacac caagaatcct ccgttttgcg acgggacgca
     1381 caaatcgctc tgagttcccg gttgaccggc gtgcagcgtg tgacacaaga ggtgaatcat
     1441 gggtgaggcg gcgagcgaga acgggtattc gatcacgctg acagagcggc ggctggtggc
     1501 ggaagggacg atggcgttct acttcgacaa gccgacgcag tttgccttca cgccgggtca
     1561 gtttgtcgac ctgacgctgc cccagccctc tgagaccgat gcggcgggca acaccagggc
     1621 cttttccatc gcgagcgcac cgcaggaatc gaccctgatg gtggccaccc ggctccgcga
     1681 taccgccttc aagcgcgaac tgcagcgtat gccgctcggc tcaaccgtcc ggatggaagg
     1741 gccgttcgga aaattggtgc tgcatgccga ccagacccgc cccgccgtgt ttctggccgg
     1801 cgggatcggg atcacaccct tccgcagcat ggcggtgcag gcggcgatgc aacggtcgcc
     1861 gcaccccatg gtgctcttct actccaaccg gcgaccggag gatgcgccgt tcctggatga
     1921 gctgcaagcc ttgcaggaca agaatcctca ctatcgcttt gtgggcacca tgaccgaacc
     1981 aacgaaatct tcccgtccct ggcagggcga gacggggtat ctcaacgcgg ctctgctctc
     2041 caaacatctc gtgaacatgg agaagccgat ctactacgtg gtcggcccgc cgggtatggt
     2101 cggcgcgttg cgcacgatgt tgaaaggcgc cg
//
LOCUS       DAOQRM010000265         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_68470_length_2064_cov_21.542061, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000265 DAOQRM010000000
VERSION     DAOQRM010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68470_length_2064_cov_21.542061"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKK81_13210"
     CDS             complement(<1..374)
                     /locus_tag="PKK81_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33894.1"
                     /translation="MHRNVISRSLLWQWTAFFISLCLALPSWADEVHLQNGDRLTGTI
                     LKMEERVLTLQTDYGGEVKIDWGKVESLKSDGTLKILVPGESHDALRDFLYGAQALRE
                     TRELGPESPVPLADITAINLEPL"
     gene            complement(526..1770)
                     /locus_tag="PKK81_13215"
     CDS             complement(526..1770)
                     /locus_tag="PKK81_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33895.1"
                     /translation="MVEPLLMNDQRRSDPVRGTIHVGWLRSLVGLLAALSLAGCLSPP
                     TLNRAVLAYDEAITDAISKQLLINIARAHHHEPIHFTGVANVAATFDFRISAGATPAL
                     TGEHGRTLVPLFGGSIAENPTISITPIEGEEFTKRILAPFQESKLTLLLRQGVDIDLL
                     LRLMAKELRLKHKGEEVAYRNSPSDKDGYDMFRKVVLHLSAIQDANHLYAEALTFERT
                     WTIPAESVTAEGFATLEQQYLITYQSETRTYTLRKPVSGRILITNYDPATLPPAERVR
                     LHETADQRPVNDVSFDIRAGHFGGEWPLQGDFRLRSFNAMLNFLGHAADEDREYAVEK
                     DARTPPVAENPVHTMDLLILDHTPDEPDLAVKSHGRYYAINAAGPQARWNREAFKLLS
                     QLFQMTVTDVPRTGVPSITIAK"
     gene            complement(1880..>2064)
                     /locus_tag="PKK81_13220"
     CDS             complement(1880..>2064)
                     /locus_tag="PKK81_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33896.1"
                     /translation="QRFHLEAWDASIIRKLQTLESIYGKMTDRATTRRMEVLEWIIIV
                     LIAISIAISFLPFTSH"
BASE COUNT          386 a          544 c          681 g          453 t
ORIGIN      
        1 agcggttcga gattaatggc ggtaatatcg gccaagggca cgggactctc gggacccagt
       61 tctctggtct cacgcagggc ctgtgcgccg tacagaaaat cgcgaagtgc atcatgtgat
      121 tcgcccggca ccagaatctt cagtgtgccg tcggacttca ggctctcgac tttgccccaa
      181 tcgattttca cctcaccccc gtagtcggtc tgcagggtga ggacgcgctc ttccattttg
      241 agaatcgtgc cggtcagtcg atctccattc tgcaggtgta cttcgtcggc ccaggacggc
      301 agggcgagac acaggctgat gaagaatgct gtccactgcc acagcaggga gcgactgatg
      361 acattgcggt gcatggatgt gaatcaggga cggacgggta cgtcactcgt ggcgagagtc
      421 gggcggcccg cgttctgtcg atggtccaac cctactggag cgccatcgga gttgcaagag
      481 agcgggaaca aaaaaaccgc gcacgggtga tccgccgact gaggcctatt tggcgatggt
      541 gatgctcggc acgccggttc gcggcacgtc ggtgacggtc atctgaaaca gttgggagag
      601 caatttgaac gcttcacggt tccagcgcgc ctggggtccc gcggcattga tcgcataata
      661 gcggccatgg gatttcacgg ccaggtctgg ttcgtccggc gtgtgatcga ggatgagcag
      721 gtccatcgtg tgcacaggat tttcggccac ggggggcgta cgcgcatcct tttccacggc
      781 atactcgcgg tcttcgtccg cggcgtgccc caaaaaattc aacatcgcgt taaagctgcg
      841 cagcctgaaa tcgccctgca acggccactc tcctccgaaa tggcccgctc gaatgtcgaa
      901 cgagacatcg ttgacggggc gctggtctgc ggtttcgtgc aatcggaccc gttctgcggg
      961 cggtagcgtg gcgggatcat aattcgtaat gagaatgcgt ccggagaccg gcttgcgcag
     1021 ggtgtaggtg cgtgtctcgc tctggtacgt gatgaggtat tgctgctcca gcgtagcaaa
     1081 gccttcggcg gtcacggact cggccgggat ggtccaggtg cgctcgaacg tcaaggcttc
     1141 cgcatagagg tgattggcat cttgaatggc cgacaagtgc aggacgactt tgcggaacat
     1201 gtcatagccg tctttgtcgg agggactgtt gcggtaggcg acttcttcgc ccttgtgttt
     1261 cagacggagt tctttggcca tgagccgcag gaggagatcg atgtcgactc cttgccggag
     1321 cagcagggtg agtttgcttt cctggaacgg cgccaggatg cgcttagtga attcttctcc
     1381 ctcgatcggc gtaatgctga tggtgggatt ttcggcgatt gatcctccga ataagggaac
     1441 caatgtccgg ccgtgttctc cggtgagggc cggtgtggcg cccgcgctga tacggaaatc
     1501 gaaggtggcc gccacgttgg cgacgccggt gaagtgaatc ggctcatgat ggtgtgcccg
     1561 cgcgatattg atgaggagct gtttggagat cgcgtcggtg atggcctcgt cataggcgag
     1621 gacggcgcgg ttgagtgtcg gcggcgacag gcagcctgcg aggctgagag cggccagcag
     1681 gccgaccagg ctgcggagcc acccgacgtg aatcgtgccg cgcactggat cagatcggcg
     1741 ttggtcgttc atcagcaggg gttctaccat atctcttcac tggagcctag cgggctgcgg
     1801 aaaaactcga ttgctacgcg atgcgagaac gccgctggcg gactgtttcc gcatcctgct
     1861 atggcctgag cccctgcgct tagtggctgg tgaacgggag aaaggagatg gcaatcgaaa
     1921 ttgcgatgag cacgatgatg atccattcga gcacttccat ccgtcgagtc gtggcgcggt
     1981 cggtcatttt tccgtaaatg ctttcgagtg tctggagttt tctgatgatg ctggcgtccc
     2041 aggcttccag gtgaaagcgt tggg
//
LOCUS       DAOQRM010000266         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_76414_length_1928_cov_17.765083, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000266 DAOQRM010000000
VERSION     DAOQRM010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76414_length_1928_cov_17.765083"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..856)
                     /gene="dapF"
                     /locus_tag="PKK81_13225"
     CDS             complement(47..856)
                     /gene="dapF"
                     /locus_tag="PKK81_13225"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNV33897.1"
                     /translation="MHGLGNDFVVVDGIRQQVALTPEQLRYLADRHFGVGCDQILLVE
                     AATQAGVDFRYRIFNADGGEVGQCGNGARCFVHFVHEQGLTDKREIRVETQGGIIVPR
                     LEGNGNVTVDMGNPRFSPDEIPFVHEDDVAIYSLDVADETLEISVVSLGNPHAVQVVA
                     SVDSAPVSEHGPLIESHERFPERVNAGFMQVVDRHTIKLRVYERGSGETLACGTGACA
                     AAVTGIRRGLLDSPVRVMTRGGDLNIAWGGPGRPVLMSGPAVTVFTGEIEL"
     gene            complement(938..1444)
                     /locus_tag="PKK81_13230"
     CDS             complement(938..1444)
                     /locus_tag="PKK81_13230"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV33898.1"
                     /translation="MTDSLNIQIRPVTPPDVPAISALAREIWQAAYPGIITQDQIDFM
                     LEQRYGHERLYDDLEDAYKWLDQAFLDGRRVGFAFSEIYRDEFKLDKLYIHPDVQRQG
                     IGGQLIEHVAGRARKMGYPCIILAVNKRNTKAIGSYQKYGFVVRESIVDDIGRGFVMD
                     DFVMEKKL"
     gene            complement(1441..>1928)
                     /locus_tag="PKK81_13235"
     CDS             complement(1441..>1928)
                     /locus_tag="PKK81_13235"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV33899.1"
                     /translation="IGKACSVYSNQSNPVFNKLLYDGRMRFNEVLLLSRQEGMRRIVK
                     ALKDGYPLFYLPDQDFGRKESIFVPFFGVPAATIPTLSRLVRLTGARVVPCIVRQVRD
                     GYEAEVMPPWENYPGESIEADTAFVNRFIETQVLRMPEQYFWVHKRFKTRPPGQQGYY
                     K"
BASE COUNT          401 a          583 c          557 g          387 t
ORIGIN      
        1 ggattgttct tcaggtaatc cgcaacatct tcgggaaagg gcgcactcat agttcaattt
       61 ctccagtgaa aacagtgacc gccgggccgc tcatcagcac cggtcgaccg gggccgcccc
      121 aggcgatgtt gagatcgccg ccgcgcgtca tcacgcgcac cggcgagtcg agcagaccgc
      181 gccggatgcc cgtcaccgca gcggcacagg cgccggtgcc gcaggccagc gtttcgcccg
      241 aaccacgttc atagacccgc aacttgatgg tgtggcgatc gacaacctgc atgaatcccg
      301 cattgactcg ctccggaaag cgctcgtggc tttcgatcag cgggccatgc tcgctcaccg
      361 gtgcgctatc gacgctggcg acgacctgaa cggcatgcgg attgcccagc gaaacgacgc
      421 tgatttccaa ggtttcatca gcgacgtcaa gggaatagat cgccacatcg tcttcgtgga
      481 caaaggggat ctcgtcgggt gaaaagcggg gatttcccat gtcgaccgtg acattgccgt
      541 ttccttcaag gcgcgggacg ataatgccgc cttgcgtctc aacccggatc tcgcgcttgt
      601 ccgtcagccc ctgctcatgc acgaagtgca cgaagcagcg cgctccattg ccgcactgcc
      661 ctacttcgcc accgtcggcg ttgaagatgc ggtaacggaa gtcaacgccc gcctgtgtcg
      721 ctgcctcgac caacaggatc tggtcgcatc cgaccccgaa atgccggtca gcaaggtagc
      781 gcagttgttc gggtgtcaga gcgacctgct ggcggatgcc gtcgacgaca acgaaatcgt
      841 tgccgagacc gtgcatcttg gagaatctga gcttcactgg ccgctccggg attgatgttg
      901 gaaaaggata gcagagacag ggcattgcct caaattgcta aagttttttc tccatcacga
      961 agtcgtccat gacgaagccg cgaccaatgt cgtcaacaat ggactcacgg acgacgaagc
     1021 cgtacttctg gtaggagccg atggccttgg tgttccgctt gttgactgca agaatgatgc
     1081 agggatagcc catcttgcgg gcccgcccgg ccacgtgctc gatcagctgg ccaccgatcc
     1141 cctggcgctg aacgtcggga tgaatgtaca acttgtcgag cttgaactcg tcccgataaa
     1201 tctcgctgaa ggcaaagccg acccggcgac cgtccagaaa ggcctgatcc agccacttgt
     1261 aggcatcttc cagatcgtcg tagagccgtt cgtggccata gcgctgttcg agcatgaagt
     1321 cgatctggtc ctgggtgatg atccccggat aagccgcctg ccagatttct cgggccagtg
     1381 ccgagatcgc cggcacgtcc ggtggtgtta cgggacggat ctgaatgttc aggctgtctg
     1441 tcatttatag tacccctgtt gtcctggtgg ccgggtcttg aaacgcttgt gcacccagaa
     1501 atactgctcc ggcatgcgca gtacctgggt ttcgatgaag cggttgacga atgcggtatc
     1561 ggcctcgatg ctttctccgg ggtagttctc ccacggcggc atgacttccg cctcataccc
     1621 gtcgcgcacc tggcgcacga tgcatggaac cacacgggcg ccggtcaggc gcaccagccg
     1681 cgagagggtt ggaatcgtag ctgccgggac gccgaaaaag ggcacgaaga tggattcctt
     1741 gcgaccgaaa tcctgatcag gcagatagaa gagcggatag ccatccttca acgccttgac
     1801 aatcctccgc atcccctcct gcctggacaa gagcaacacc tcgttgaaac gcatccggcc
     1861 atcatagagc aacttgttga acaccgggtt cgactgattg gaataaacgc tgcacgcctt
     1921 gccgatca
//
LOCUS       DAOQRM010000267         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_82496_length_1838_cov_17.357824, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000267 DAOQRM010000000
VERSION     DAOQRM010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82496_length_1838_cov_17.357824"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKK81_13240"
     CDS             complement(<1..194)
                     /locus_tag="PKK81_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNV33900.1"
                     /translation="MTADAAHRLACEGTALLWRGDFQNARQLLNAMARRADRHPPKPG
                     TSPAESFHFHRQTQSRRARIL"
     gene            complement(416..>1838)
                     /locus_tag="PKK81_13245"
     CDS             complement(416..>1838)
                     /locus_tag="PKK81_13245"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV33901.1"
                     /translation="RETAVESLEEDLESARAGMSHSASREAESTRLAADLQLKIADAQ
                     AQEEATRQRMNELERQLQSLRAATISSVPLGNVEQETLRRECEQVGIVTGAATILTMF
                     RDLKKGARSTVTVLITGEPGTGKELFARAVHRLSPRAPKPFIAVNMAAISPELFESEL
                     FGHVRGSFTGALMDRKGYFELAHHGTIFLDEIGDLRLDHQSKLLRVLQDRTFYRVGAT
                     TPTTVDVRIVAATNKDLQRGVSEGWFREDLYFRLKGLVLHLPPLRDRPDDIPPLAEAF
                     LREAANQPQHANIRLSEEALATLQQQAWRGNVRELRQCLEQAIALSDGPIITTADLRL
                     SGQPSPTVVVTGAEPLLPDPAGDIAVLNQLRQHRFDMQATAKALGWDRSTVTQRLKGL
                     CFQALVESGRDQTKAASALAGDPSLLRAVELKLMDYYGHLMETIEPFTTAEDALLDCK
                     RRFKNLPERHFKSVEVLVRQHFG"
BASE COUNT          318 a          523 c          608 g          389 t
ORIGIN      
        1 aggatgcggg cgcggcgaga ctgggtctgt cgatgaaaat ggaaggactc tgccggcgag
       61 gtgccaggtt tggggggatg gcggtcggca cgccgcgcca tggcgttgag gagctgccgg
      121 gcattttgga aatcgccccg ccagagcagc gcggttccct cgcaggccag gcggtgcgca
      181 gcgtcggcgg tcatactgtc gtccgcaata gttacacgcg cgggcggtgg agctccccat
      241 tccgagcgcc agcgcgcgga gcagggctgc cctgactcgg tccaaagcac gaagggtgtc
      301 ttgctcatga aaggaccttg cctgcggtgc ttcgtatgtc tgaacgaatt ttaaacaagg
      361 tgttgttcat aaatgtggtt ctggtccggg atgagattcg gtgagtcgag gtattctacc
      421 cgaaatgttg ccggaccagg acttcgacgg atttgaaatg ccgttcgggg aggttcttga
      481 agcgacgctt acaatcgagc agggcgtcct ccgcggtggt gaagggctcg atcgtttcca
      541 tcaagtggcc gtaatagtcc atcagtttca gttcgaccgc gcgtagtaac gaggggtctc
      601 ccgccagggc cgaagctgct tttgtctgat ctcttcccga ttccacgagt gcctgaaaac
      661 agaggccctt cagccgttgt gtgacggtgc tgcgatccca ccccagggcc ttggctgtcg
      721 cctgcatatc gaaccggtgt tggcgcagct ggttcagcac ggcgatgtct ccggccggat
      781 cggggaggag cggttcggct cccgtcacga cgacggtggg cgaaggttgc cccgagaggc
      841 ggaggtcggc tgttgtgatg atcggtccgt cggagagcgc gatcgcctgt tcgagacatt
      901 gacgcagctc tcgcacgtta cctctccatg cctgctgctg aagcgtcgcc agcgcttctt
      961 ccgagagcct aatgttcgcg tgctgcggct ggttcgcggc ttcccggaga aaggcttccg
     1021 cgaggggagg aatatcgtcg ggccggtccc gcaacggcgg gaggtgcagg acgaggccct
     1081 tgagccgaaa atacaggtcc tcgcggaacc agccttccga cacgccacgc tgaagatctt
     1141 tgttggtcgc ggccacgata cggacatcga ccgtggtagg agtcgtcgct ccgactcgat
     1201 aaaaggtccg gtcctgcagc actcgcaaca gtttgctttg atggtcgagt cgaaggtcgc
     1261 cgatttcgtc cagaaagatg gtgccatgat gcgccaattc aaaatagcct ttgcgatcca
     1321 tcagcgcgcc ggtgaagctg ccgcgcacgt ggccgaagag ttcactctcg aagagttccg
     1381 gggagatcgc cgccatgttc acggcgatga acggcttcgg tgcccggggg ctcagacggt
     1441 gcacggcacg cgcaaacaac tctttgccgg ttcccggctc gccggtgatc aggaccgtga
     1501 cggtggaacg ggcgcctttc ttgagatcac gaaacatggt caggatcgta gcggctccgg
     1561 tgacgatgcc cacttgctcg cattcccggc ggagtgtttc ctgttctaca tttcccaggg
     1621 gcactgaact gatggttgcg gcccgcaggc tctgaagctg ccgttcgagc tcgttcatgc
     1681 gctgccgcgt cgcctcctct tgcgcctggg catcggcgat tttgagctga agatcggcgg
     1741 cgagtctcgt cgattcggct tctctcgagg ctgagtggga catgccggcg cgagccgatt
     1801 cgaggtcttc ctccagggat tccactgcgg tctccctg
//
LOCUS       DAOQRM010000268         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_91572_length_1725_cov_10.789222, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000268 DAOQRM010000000
VERSION     DAOQRM010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91572_length_1725_cov_10.789222"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1725)
                     /locus_tag="PKK81_13250"
     CDS             complement(<1..>1725)
                     /locus_tag="PKK81_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV33902.1"
                     /translation="LVAGALIGPHGLHLIPDVSQVQVLAEIGIVLLLFTIGMEFSSAH
                     FAAARRILMVAAPVQTGGVLVLALLGALAVGLSYQQGIFWGFLLSLSSTAIVLKALSE
                     QGESDSFHGRATVAILIFQDLAVVPMMLVTPILGTPSGSAMGTVLMTLVKAAIVVGLI
                     VAAAWYLVPRLLRHIVRSRSRELFLLSIIVLCLGIAWLTSLGGLSLALGAFIAGLVIS
                     ESEYSHQALAEVLPFRDSFNSLFFVSIGVLMDIRVVLEHPFIVLGLLLAVIVGKLVTG
                     SGAMVAAGAPPRSSVLVGVALAQVGEFSFILAQQGQDAGLFTGDQYQLFLAVSVLTMV
                     VTPFLMQWSPDLGRRIEAMQRVRGWMPTRTAAHVEQMEGSQIRIKDHVIIVGYGLNGR
                     NLARVLSETEIPHVALDLDGETVRREARHGVPIYYGDGSNANVLRHMKIDDAKVLVVA
                     LSDPFTARRTVKVAKGLNSKLHIVVRTRYLRELEELHQLGADDVVPEEFETSIEIFAL
                     VLRTYSLPQEFVARKAEQIRREGYALLRRSEMPELAHHLRGGTLTDVEVETCRIDDEA
                     PAVGKSLT"
BASE COUNT          404 a          591 c          451 g          279 t
ORIGIN      
        1 ccgtcagcga ctttcccacc gccggagcct cgtcatcgat ccggcaggtt tccacctcga
       61 catcggtcaa cgtgcctccg cggagatgat gagccagctc cggcatctcg ctccgccgca
      121 acagcgcata cccttcgcgc ctgatttgtt ccgcttttcg tgccacaaat tcctgcggca
      181 aactataggt gcgcagcacc agggcaaaga tttcaatcga ggtctcgaac tcttccggca
      241 ccacatcgtc cgcacccaac tgatgcaact cttccaactc tctcaaatat cgcgtccgca
      301 cgacgatgtg gagcttcgaa ttcaatcctt tggccacctt caccgttctc cgggccgtga
      361 acggatcgga caaggccacg accagcactt tggcgtcgtc gatcttcata tgccgaagca
      421 cgttggcatt cgagccgtca ccgtagtaga tcggcacgcc atgacgcgcc tcgcggcgca
      481 ccgtttcgcc gtccaggtcg agggccacat gcgggatttc ggtctcgctt aacacgcggg
      541 cgaggtttcg accgttgagc ccatagccca caataatgac gtggtctttg atccggatct
      601 gggaaccttc catctgctcg acatgcgcgg cggtgcgggt cggcatccag ccccggacgc
      661 gctgcatcgc ttcgatccgc cgccccagat cgggcgacca ttgcatcaaa aacggcgtca
      721 ccaccatggt cagcaccgac accgccagaa ataactgata ctgatccccg gtgaacaaac
      781 cggcatcctg cccctgttgc gccaggataa aactgaattc gccgacctgc gcgagcgcca
      841 ccccgaccaa caccgacgac ctaggcggag ccccggccgc caccatcgcc ccactcccgg
      901 tgaccagttt cccgacaatc acggccaaca acaggcccag cacaatgaac ggatgctcca
      961 gcaccacccg tatgtccatc aagacgccga tcgacacgaa aaacaggcta ttgaaactgt
     1021 cacggaacgg caggacttcg gccaatgcct gatggctgta ttccgactca gaaatgacca
     1081 gcccggcgat aaacgcaccc agggccaacg agagtccgcc cagcgacgtc aaccaggcaa
     1141 tcccgaggca gagcacgatg atcgagagca gaaacagctc acggcttcga ctccgcacga
     1201 tatggcgcag gagccgcggc acaagatacc aggctgccgc aacgatcaac ccgaccacaa
     1261 tcgccgcctt gaccaaggtc atcagtaccg tccccatcgc actcccgctc ggagtgccca
     1321 ggatcggcgt gacgagcatc atcggaacga cggcaaggtc ctggaagatc agaatcgcaa
     1381 cggtggcgcg accgtgaaac gaatcactct caccctgttc cgaaagggct ttcagcacga
     1441 tggccgtgct gcttaatgag aggagaaagc cccagaaaat cccttgttga tacgagaggc
     1501 ccaccgccaa agccccgagc aacgccagga caaggacgcc cccggtttga accggcgcgg
     1561 caaccatgag aatgcgccgg gcagccgcaa agtgtgcgga ggaaaattcc atcccgatcg
     1621 taaagagcag cagcacgatc ccgatctcag ccaggacctg cacctgcgac acatcgggaa
     1681 tcagatggag cccgtgcggc ccgatcaggg ctccggccac cagga
//
LOCUS       DAOQRM010000269         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_1192
            NODE_102920_length_1609_cov_25.109395, whole genome shotgun
            sequence.
ACCESSION   DAOQRM010000269 DAOQRM010000000
VERSION     DAOQRM010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852535
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:19:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,629
            CDSs (with protein)               :: 2,629
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102920_length_1609_cov_25.109395"
                     /isolate="AMR_MDS_1192"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1590)
                     /gene="purH"
                     /locus_tag="PKK81_13255"
     CDS             complement(37..1590)
                     /gene="purH"
                     /locus_tag="PKK81_13255"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNV33903.1"
                     /translation="MASIARALISVSDKTGVVEMAKGLAALGAEVLSTGGTAKALREA
                     GVAVTDVAAYTGSPEILDGRVKTLHPKIHGGLLGRRRVPDHVKQMQQHGIGNIDVVVV
                     NLYPFESTIAKPNCPFEEAIENIDIGGPSMLRSAAKNHEDVLVLVDPADYGRVLEALK
                     SGSVTPALRRELAMKVFQHTARYDSLIAGYLEKQVQGSEVKFPAILSLQFERVETLRY
                     GENPHQQGAFYREMNAKEPAVSRGKILHGKAMSYNNFLDSNSALELVKEFEQTAVAII
                     KHNNPCGCALGATPVDAYVKARATDPVSAFGGVIAFNRPVDLAAAKEITSTFVEVVIA
                     PGFAEDALAELKRKKDIRLLDVGPLSKATAEGYDLKKLVGGLIVQDRDLGMIKDIKAL
                     AVPTSRKPTEEEYAACAFAWVVCKHVKSNAIIYGRPGEIVGIGAGQMSRVDSVKLAVM
                     KAQSPVKGCVMASDAFFPFRDGIDAAAEAGITCVIQPGGSIRDPEVTKAVDEHGMAMI
                     LTGMRHFRH"
BASE COUNT          316 a          525 c          445 g          323 t
ORIGIN      
        1 gccgagaacg aagttggagg cccgtttcaa cgcccctcaa tggcggaagt ggcgcatgcc
       61 cgtgagtatc atcgccatcc cgtgttcgtc cacagccttc gtcacctcag ggtcgcggat
      121 cgaaccgccc ggctgaatca cacaggtaat gccggcttcc gctgccgcgt cgataccgtc
      181 acggaacgga aaaaaggcat cggatgccat cacgcagccc ttcacaggcg actgggcttt
      241 catgacggcc agtttcaccg agtccacccg gctcatctgc ccggctccga ttccgacgat
      301 ttcgcccgga cgcccgtaga tgatggcgtt cgacttcaca tgtttacaga ctacccaggc
      361 aaacgcgcag gcggcatatt cttcctcggt cggcttgcgc gaggtcggaa ccgccagagc
      421 cttgatgtcc ttgatcatgc ccaaatcgcg atcctgcacg atcaatccgc cgaccagctt
      481 cttgagatcg tacccctccg cagtcgcctt gctcagcggt cccacgtcca agagacggat
      541 gtccttcttg cgcttgagtt cagccagcgc gtcctcggcg aagcccggcg cgatgacgac
      601 ctcgacgaag gttgaggtga tttccttggc cgcagccaaa tccaccggac gattgaaggc
      661 aatgacaccg ccgaacgccg acacggggtc ggtcgcgcgc gccttcacat aggcgtccac
      721 cggtgtcgca ccgagtgcgc agccgcaggg gttattgtgc ttgatgatgg ccaccgcggt
      781 ctgctcgaac tccttcacga gttcgagcgc cgagttggag tcgaggaaat tattgtagga
      841 catggccttg ccgtgcagaa ttttcccgcg cgacacagcc ggttccttgg cattcatttc
      901 acgatagaac gccccctgct gatggggatt ttctccgtag cgcagcgtct ccacacgttc
      961 aaattgcagc gagaggatgg cggggaactt cacctcactc ccctgcacct gcttttcgag
     1021 atacccggca atcagactgt cataccgggc ggtatgctga aacaccttca tggccaactc
     1081 gcggcgcaac gcgggggtca cggaaccgga cttcagcgcc tccagcaccc ggccgtagtc
     1141 ggcgggatcg accaggacca gcacgtcttc atgattcttc gcggcggatc gcaacatcga
     1201 cggaccgccg atgtcgatat tttcaatggc ctcttcgaac ggacaattgg gcttcgcgat
     1261 cgtcgattca aagggataga gattgaccac caccacatcg atgttgccga tgccatgttg
     1321 ctgcatctgc ttcacatgat cgggcacgcg acggcggccg aggagtccgc catgaatttt
     1381 cgggtgcaac gttttcacgc gcccgtccag gatctcaggc gaccccgtat aggcggccac
     1441 gtcggtgaca gccacgcctg cctcacgcaa ggccttcgcg gttccaccgg tggacaacac
     1501 ttcggcgccc agggccgcca gccccttcgc catctcgacg actccggtct tgtcagaaac
     1561 actgatcagt gcccgcgcaa tgctggccat gatgaactcc ttcaactat
//
